LOCUS       DAOOSJ010000001        48176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_571_length_48176_cov_5.708319, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000001 DAOOSJ010000000
VERSION     DAOOSJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 48176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48176
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_48176_cov_5.708319"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2514
                     /gene="secA"
                     /locus_tag="PLG14_00005"
     CDS             <1..2514
                     /gene="secA"
                     /locus_tag="PLG14_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOX47536.1"
                     /translation="GERPFDTQIMGGIVLHQGRIVEMKTGEGKTLCSVGAAYLNALSG
                     DGVHVVTVNDYLAERDSQWMGQVFRFLGVSVGCVLSKMDNEARVAAYRADITYGTNNE
                     FGFDYLRDNMTWSPEGRVQRGHNYCVVDEIDSILIDEARTPLIISGPADDDTFKVNEV
                     GKLASQLVEVKKDPETGEYPKEEEGEVLEGDYKVDEKSKRISFTSDGMTKMEGLLQKR
                     GLIKGSLFDEGNFEFVHYFTQAARAQILFQKDVDYVVQEGLVQIVDEFTGRILHGRRY
                     SDGLHEAIEAKERIKIARRNRTLATITFQNYFRMYKKISGMTGTADTEAPEFQKIYNL
                     DVSVIPTNRPVARADEDDLVFLNEAEKFEAICDEIAEAHKKGQPMLVGTVSIEKSEHL
                     SSLLTRRGVRHEVLNAKNHAREALIIAEAGAKGSVTIATNMAGRGTDIKLGGNPEFRA
                     RRRAGSQASDEEYKAALALEYEAWRKDYEEVRSLGGLYVLGTERHESRRIDNQLRGRS
                     GRQGDPGRSAFFLSLDDDLMRLFGGENLKGLMSKIGMKPGEPIYHPLLNRTIENAQKK
                     VEERNFEIRKHLLEYDDVLSEQRKFIYEQRDAILKETDLLSRVRDSASDMLDSFLEAF
                     SDAQRKDQGRALSELQTALKDAFNVQLPFRADELSGKAREGLKASISELLERDLSGKL
                     EYAGPENLNHFIRFQYLQAIDQKWLDHLEQMEALREAVYLRSYAQKNPLLEYKLEGFD
                     IFNEMIEEIRRSIASRVYLVRIQTAEERRARPVSERAGNAVHEDAASFAGAGSGAKSA
                     VAEAARPAGATVVRGTEKVGRNDPCPCGSGKKYKHCHGQ"
     gene            2519..4228
                     /locus_tag="PLG14_00010"
     CDS             2519..4228
                     /locus_tag="PLG14_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOX47537.1"
                     /translation="MSLNHREIDLVLAELDLEGAQLQRVVQPSYDSVVLGFYKSGTES
                     ELLISIAHGACRIHTLSSRPPKPQRPLRFMECLRSRLRGGRLESIVQLGDERILRLDF
                     LVVRKEGAEEEGERSYRPIGAYARDLAHGSARVAIHGATDGAAQAAAQGAARDEAGPA
                     ESDGGEAAPEGGTARRYRLYARLWSGAGNLILCEEDGTIVDVLARRPKRGEISGECCA
                     IEEELAAARVASGAGDKGAARGAEGAAGASGPARPARTFEPRDFPPAPRREDGGSYNE
                     RIEAFYASQGGELSRERLLEAARERFSKRERAIGARLAELEARAAEYRGGERFRELGD
                     ILMANQSAAPEGRFLPCEDFYRGGMVDIPLDPRKSVVENAQVFYERFRKAGSGLSELE
                     SEIASAKASLAEAKAELERLESLKEPLLIARALQKGGTAREPKKRPYPGLSLERDGWT
                     ILVGRSAKENDELLRRHVRGSDLWLHARDYSGSYVFVKARAGKSFPLEIMLDAGNLAI
                     YYSKGRANGGGELYYTQAKYLRRAKDGPKGLVIPTQEKNLRVTLDEKRLRELKELIGD
                     EGD"
     gene            complement(4225..5499)
                     /locus_tag="PLG14_00015"
     CDS             complement(4225..5499)
                     /locus_tag="PLG14_00015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX47538.1"
                     /translation="MRRIKGRRMAFEDPAERRAYLILAMSFNAALVLCLAGLFAGMAL
                     RNEELVRETVLARARSLFEEIVLTRKWNSLRGGVYVLKGPGVESNPWLADPDIAAADG
                     RVLTKRNPALMTREISELAENVASFRFRITSLRPVNPSNAPDPFEKAALEGFERGGSE
                     TWAREARPDGAWFRYMGVLKADASCLACHAAQGYKAGDVRGGISVSFPVSAIDREKRR
                     TYLAIAGTGLLVTVLMLGVFHLLMRGFRLKLEKARSELREAAVTDALTGLHNRKYVLA
                     RFDEAVAEAKRSGAPFSCAIIDADNFKRINDELGHQMGDAALVSLAAVMKETLRPYDV
                     VGRYGGEEFLILFPSSPLGAALAACERLRLAVESGLEARTPGLGRRLTISAGLASLGG
                     GVDSTLTILERADSALYRAKAAGKNRCETERR"
     gene            complement(5511..8021)
                     /locus_tag="PLG14_00020"
     CDS             complement(5511..8021)
                     /locus_tag="PLG14_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOX47539.1"
                     /translation="MFKGLTHRAQRVLTTLAQEEAKRSRSDQLQPEHVLLAILREGEG
                     LGFKALRSLKIDPLELRDELERSLERKRAGFVLGDLPLSRRSRSLLETAAEEANMSSS
                     EYIGTEHFVVAAAREQGAAFERFLSRYGVYYEQLRAAVRLLREPGQGAEERADARRPP
                     EERKAPAPAPRGQAPARTPLLDEHARDLTALAREGRLDPVVGREREIGRVVRILARRT
                     KNNPVLVGEPGVGKTAIVEGLALRFARGDAPDELSRKRLLALDIASVVAGTKYRGEFE
                     ERLKRIMKEIALAGNVILFIDELHTVIGAGGAEGSIDASNMLKPALSRGEIQCIGATT
                     LDEYRKYFEKDAALERRFQAVLVEEPSQSETLEMLEGIKSRYEEYHGVAYSRGAVEAA
                     VDLSKRYLADRFLPDKAIDLLDEAGALKKVTNPSRPPELSEIEEEIRRLTEEKLALVS
                     TQNYEKAAETRDRVRGLRARLEALKAEWESGGRGGPAPVTEDDLRLVLSEATGIPLGR
                     LAENEAERLLRLEEALHGRVVGQEEAIAAVSSSVRRSRAGVSDGKRPLGSFIFLGPTG
                     VGKTLVAKCLAEILFGSEEALIRIDMSDFMERHNAARLVGSPPGYVGYEEGGMLTEKV
                     RRRPYSVILFDEIEKAHPEVFNILLQLLEEGELRDNLGHAVSFRNAVVIMTSNAGARE
                     ISSGGLGFRPEDRLLSYAEVRSSSLSELKRRFNPEFVNRVDDIVVFKPLSRDEVRKVL
                     ELLLGELSRRVALKGIVLEVSPSAKDWLAEKGYEPAYGARPMRRLLQSEIEDRLAEEI
                     LAGRLGPGSVAKVELREGAIRVRPKPDPKREPALKGRG"
     gene            complement(8033..9865)
                     /locus_tag="PLG14_00025"
     CDS             complement(8033..9865)
                     /locus_tag="PLG14_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47540.1"
                     /translation="MICAICGEREAALFIRRSRGDSSEDLALCEECAKRRGLSAGNGR
                     IELRIEELLAPEAEAGTGLPAACPSCGLGVEELRREGRLGCPSCVATFRAELGRILRS
                     SAKPPFFRGVAPAAGAESAVRAAPAHKPAPEPGPEARTGELAEARLSEPALEASLEAA
                     LAAEAYELAASIRDSLAKLNAAGAAPGSANPPTAAAAAPSATAAPPAGAGPAVPSAAE
                     GPAAAGGAFPAAAAEPFALIRGAPGFVLSGGEGSDADVVLWTKARVARNLSGLAFPGA
                     GSESAPSRKIAAPVAAALEDWRLLSLASAGEAARRALVETGFVSRAYALDPGALLAYS
                     GREPFYCLFDEIDHLRLVARLPGLSPQAALGLALSRAAAFEAAAGARGFEPAFDEEFG
                     YLSARTADLGSGVALTATLHLPALAVAGLAERAFRPVLAAGFGLRGFYGAETGSAGDL
                     YEVSTERAYGLSPAELAEGLEAVLRPLVKAERRARAELAGPRREDLLDLAGRALGLLR
                     YCRRLPAAEAQAHVSALRLALLAGVAESGVAEGGVAEAGVAQAVAAGGSAPAATPEAL
                     AGLLVSLGPGCLALRGASALADAAEAERQRARLAERAVAALVLS"
     gene            9943..10923
                     /locus_tag="PLG14_00030"
     CDS             9943..10923
                     /locus_tag="PLG14_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX47541.1"
                     /translation="MTRRYAFRASALALLAFGAAFFLPAQEAGKGQAVRLGALKGPTG
                     IGMIHLFETPPELPGGGRLEPVAVPNAEVMTAKAVSGEYDFAVLPLNMAAKLYSAGIP
                     LSLAAVVGDGMVSFLTSDPAVSSLADLKGKEIFVAGQGATPDYLLRRLLKETGLDPEK
                     DLRLAYSLPYPEMAAALAAGKISTAVLPEPFATLALQANPALKSPLDLGALWTKATGQ
                     KSYPMSALVVSRKLAAERPEAVRAVLAAVKSSIEAVKADPASAGLLVEKHELGLKAPV
                     AAKAIPRSAYVFVPAPGARPAVEALLRVFLETSPASIGGKLPDGGFYAAF"
     gene            10994..11662
                     /locus_tag="PLG14_00035"
     CDS             10994..11662
                     /locus_tag="PLG14_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX47542.1"
                     /translation="MGKELILPPPERVFAEALVLYPSPRFLAALFGTFLRGLAAFGVS
                     VLAGAAAGLAAGLSPLVEAALAPLLTLIRATPVLALILVALLWFPSGAVPVFAAFLMA
                     FPVMATSAAEGARAADPRLLEMARLFRVPRASVFRKIRLPAAAPHLLAGAKSALGLSW
                     KVVVAGEVLSQPLRALGTGMQDARVLLETPRVLAWAAATVALCGLTEWAFGHAAKRAL
                     RHVL"
     gene            11652..12371
                     /locus_tag="PLG14_00040"
     CDS             11652..12371
                     /locus_tag="PLG14_00040"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX47543.1"
                     /translation="MSYELRAARKSYDGQPVLDGLDLSIGKGEILVVLGPSGCGKTTL
                     LNVLAGLSPLDSGLRLGFEAARFSYAFQEPRLLPWLTARENAAFALSGALPREEALSR
                     VDALLEAAGLSAAAQRRPGELSGGMRQRLSLVRAFAYPAEILLLDEAFESVDLKTKIE
                     LMDAFLGLWKRERPTAVCVTHDVEEAAYLADRAIVLSPRPARVVDDFAIEAPRELRSL
                     GSEATMEAEARLYRLVLGAGR"
     gene            complement(12430..14379)
                     /locus_tag="PLG14_00045"
     CDS             complement(12430..14379)
                     /locus_tag="PLG14_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX47544.1"
                     /translation="MASLPESPAIAVVEDEYIVALDIKNFLERSGYRVSGLFPSGDEL
                     LARMDELGPDLVLMDIKIRGSLDGVETAKIVHDRWKTPVILLTAFADDETIARAKLTQ
                     PFAYILKPFEERELRTSIEIALYRAGMERRLRDSEERFRRLFQEGISGNFLADAQDRV
                     IEANAAFRRLIGLEADAPLPRLREIFHDSASWESFKAGLARSRRVEAAELALRKPGGG
                     ELIVLANAALLTDGDGNAAGIQGEFSDLTDRRVLEERLGQSQKMEAVGRLAGGVAHDF
                     NNILTAVLGYANLLSESAEKYPELKDDVEGIRKAAAKAAALTRQLLAFSRQQPVAPKN
                     LALNALVGDMERMLRRLLTEEISLSFDLGEGLPEIFADPTHVEQVVVNLVVNAKDAMP
                     GGGRLLVSTRLESLAVPRAVGLETLAPGRYAALSVRDSGGGIAPEILPRIFEPFFTTK
                     ARDKGTGLGLSTVYGIAKQAGGAVGVSSASGEGSTFTVYFPETRRPQAGAAGGPASSS
                     EDQREALARRPGTILFVDDDEEIRLLAGKFLGRRGHRVLVAANAGEAILLAESGGSPI
                     ELLVTDLVMPIMDGYRLARRLAGAIPGLKVIFISGHPERATDKAADGASGKAAAGSPP
                     GRFLPKPFTEQELSEAVDEALAEPS"
     gene            complement(14380..14784)
                     /locus_tag="PLG14_00050"
     CDS             complement(14380..14784)
                     /locus_tag="PLG14_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX47545.1"
                     /translation="MKILVVEDDFGSRRMMQKLLAPYGDVDVVVDGEEAVQAFRVAWD
                     EYKPYDLVFMDIMMPKMDGHEALKRIRELERELGVKPAEEAKIVMTSVLEDPRNVIEA
                     YYQGGATSYLVKPIDRERLREEIAKLGFAPRE"
     gene            complement(14806..16896)
                     /locus_tag="PLG14_00055"
     CDS             complement(14806..16896)
                     /locus_tag="PLG14_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX47546.1"
                     /translation="MRKGGILIVEDENIVALDMRMRLEALGYGVRAVVDSGPAALEAM
                     TGPEPPDLVLMDIKLKGAADGIETARAARELVDLPIIFVTAFTDEKTLERAKLASPYG
                     YIVKPFHERELRIAIELALYKFQYELSIRKAKEFAEETSRLKGEFLANVSHELKTPLN
                     SVIGFTELALDKAADEEQRDYLSSALAGAKTLSALIDSILDFASLESGRLSAELEAFS
                     LAGLLDGIVDVLAVGAHSKGLEATFRLDPSLPAFLVGDPGRLKQALHNLADNALKFTE
                     RGGVRLEVGRLPEGAAAGRPDEFRMGASSGAAFPKPPPGGLLLGFALEDSGIGMPAER
                     IGEAFGRFTQLDGSRTRSAGGTGLGLAIVARSVELLGGDVAVRSAPGKGSRFELILPF
                     GLPAGSAEPGGDGKASADGAGGRPLSGLRIGLLGFEGEARRDLEELLSSLGAAALPGQ
                     AGAEGPALDCLVADELALAGAAVKGGAGALDGARPLPLVVARRFGGSGPAKRREGPVL
                     PLPVRARHLVSALLELGVGDRDLCHAWLDGHAEAPLGSSKAATRGPARSAARGASAAV
                     PVAPAAPAVAAPAARGAPLAKGAAPSAARGASVAAGGTPVAAVQGELAEGAELGNLAA
                     RLEASLESLDFAAAERVSKEYRELFVARGAESCERLAFSALLLSRKGDGEGLRGVIER
                     ARRLGAAAVPRVDS"
     gene            complement(16893..17414)
                     /locus_tag="PLG14_00060"
     CDS             complement(16893..17414)
                     /locus_tag="PLG14_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX47547.1"
                     /translation="MNGEKSGRYLSFELGGESYALEVERVEVVLEPMPITRVPKAPAH
                     LRGVINYRGTVIPVADLRSRFGGLAQELAEAEGKALAAAAASEAEPEGKGIVVLHIDY
                     GGDDLTIGVLADAVREVVDIDPSRIDRAPRFGGRGQEGLISGIAEKDGRFIVILDVDA
                     AFADAAKASGEAR"
     gene            complement(17431..17799)
                     /locus_tag="PLG14_00065"
     CDS             complement(17431..17799)
                     /locus_tag="PLG14_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX47548.1"
                     /translation="MAKKILVVDDSAAIRQSISFILKQEGYDTVEAQDGLDALNVLGT
                     LDAVDLIITDVNMPNLDGIGFIRKARELPKFKFTPILVLTTESQGSKMNEGKEAGATG
                     WIVKPFNADKLLGIVKKVAE"
     gene            complement(17859..18968)
                     /locus_tag="PLG14_00070"
     CDS             complement(17859..18968)
                     /locus_tag="PLG14_00070"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOX47549.1"
                     /translation="MKQTIKVMLVDDSAVVRQTLREVLESDPQIEVVNASADPIFAQK
                     HLETMRPDVIITDVEMPRMDGLTFLRSIMQTAPLPVVVCSSKTEEGSENAIKALEYGA
                     VEIIQKPKLGTKQFIEESKVRICDAVKAAYLSRNKLSSLVAPARPEAGRQRSLDREVS
                     PKLTADVMLPKPEAGRVFVETTEKVVVVGASTGGTEALREFLELFPEDAPGIVIVQHM
                     PEHFTAAFAMRLDGLCRINVKEAEDNDTVIRGRALIAPGNRHTLLKRSGARYYVEVKE
                     GPLVCRHRPSVDVLFRSAARYAAKNAIGVIMTGMGDDGARGMKELHDAGAQTIAQDEA
                     SCVVFGMPNEAIKLGGVDVILPLGGIAQEVQRRAR"
     gene            complement(18990..19853)
                     /locus_tag="PLG14_00075"
     CDS             complement(18990..19853)
                     /locus_tag="PLG14_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOX47550.1"
                     /translation="MSEPGSYRAVPGVAPPSSLGDGDFRRLAGFIESELGIRMPDTKR
                     VMLESRLQKRIRHFGMSGFTEYVDFVFSPQGRESELINMIDAVTTNKTDFFREADHFE
                     YLVESILPAAAARGGTMSFWSAGCSTGEEPYTLAMVLEEFREANPRFSYRILASDLST
                     QVLDKAKAAVYEEEKAAPIPMSFKKKYLLRSKDPASGLVRIKPELRAKLSFARINFMD
                     ERYPIDGLFDVVFCRNVIIYFERAVQELILGRICRYVRPGGWLILGHSETLTGMDLPL
                     RSVAPTIYSRA"
     gene            complement(19850..22117)
                     /locus_tag="PLG14_00080"
     CDS             complement(19850..22117)
                     /locus_tag="PLG14_00080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOX47551.1"
                     /translation="MIDKFKAAFREESYELLSQLEDTLLELESQPLNGELINAAFRAI
                     HTVKGSSAMFGFETISKFTHEVESVLDQCRNGTLPITKDFIGLTLKARDHLRRLLEFD
                     EPPPAEVSGDGEGILLRYREYAATDAGRKPQAAAAEAAAQAAPAAPQSSAPSAPAASA
                     APRAASQPQAAAAQAPAQAQAPAAEPAAGAAEAPAAPSGLVTYRVFWRPKPDIFMDGT
                     KALNLVEELSSLGSAAVYPHTEAIVSLDALDPEKCVCYWDVLLTTAKSENDLRDVFIF
                     VEDSSELRIERVEEAPLDEEGRSKRLGEILVDRGIVSADSLREALGSQKRLGEVLVEK
                     HIATKPEIESALVEQEHLKKVQERGEAGMGSVRVASDKLDALVDLVGELVTLQARLSR
                     TSLDLQDGPLSSISEQFERLIAQLRDNTMSIRMLPIGSTFNKFRRVVRDLSLELGKEA
                     ELLTEGAETELDKTVIEKLGDPLVHIIRNSLDHGIERPDVRRAAGKPEKGSILLSARH
                     SGAYVLIQVTDDGKGLDKDAIYGKAVERGILSPGQEIADEDLYLLIFAPGFSTAKVVS
                     AVSGRGVGMDVVKREIDSLGGSVSLQTEKGKGTTVTLKIPLTLAIIEGLLVRIEGEHY
                     VIPLSSVDGCIEIRREELAAYGDRRILSYRDELVPFLPLREAFETPGEEPEIEQIVIA
                     AAQDAKIGFVVDQVVGDYQTVIKPLGRMFKDVEGVSGATILGDGTVALILDVNRLSYA
                     VQRESARKAAKGSER"
     gene            complement(22133..22462)
                     /locus_tag="PLG14_00085"
     CDS             complement(22133..22462)
                     /locus_tag="PLG14_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX47552.1"
                     /translation="MPTPKKAAPKTLALSGAYTVDRAAGLKSELQALLAGQEPAFVSL
                     SELEELDLACLQVLYAARRSAKASGREFHFAGSVKPRVAKRLVACGFLRGFVERAEDF
                     EAGLLDF"
     gene            complement(22572..24611)
                     /locus_tag="PLG14_00090"
     CDS             complement(22572..24611)
                     /locus_tag="PLG14_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOX47553.1"
                     /translation="MRPSAARLRLALRAPAAILLAVLLGSCASPGPAPKGESPALSYE
                     RLKLEEIRAFLKDDPAAAAEAALYFLARPEALADGVAPGGRESLVLELKAIVSEAGSG
                     FAARHEAALAAKDPRGALSALRSLKALGAFPASASLLRPEAAALAGAPAAREAELLVA
                     EAEAFYGKGLSTPALLLYLESLDARGLGAAEAAGSAMSAAGAAGKAGAAGSAGDGAAT
                     GAPLAADAAPKEELLLWAGRALEARNRAVLSRLGRELAARGLGLPEKALALLSSRDSM
                     ADMRKGVVTIRVDRGIKIEQGLGSPDRMLGSGFYIDGGGYVLTNYHVIESEVDPKFEG
                     YSRLSVRPSDAPEARIPAKVVGWDRLLDLALIKVEARPEYVFPVGAGAAPGPGDRIYA
                     IGSPLGLENTVTAGIVSASGRRLLQTGEAMQVDAALNPGNSGGPLVDESGALAGVVFA
                     GMPQFQGLNFAIPAAWAARVLPALFRGSEVERAWLGLSLADLGSASPNSEGGKALEIS
                     YRHPSAPPGLEAGSRLLSVDGASFGKIVEAQAYLLSRSPGELALVKTGGADPKGEASS
                     ALRALRKRPFSPLEAAASLDRRDLLFPALFGMSVRPLPGSFLEPEAYSVAKIYPGSIA
                     DESGLSENDPFALRRFIVDKELRAAVIQIHVKKRKAGFLESIIQIPAPLDIPDFI"
     gene            complement(24608..26692)
                     /locus_tag="PLG14_00095"
     CDS             complement(24608..26692)
                     /locus_tag="PLG14_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47554.1"
                     /translation="MRAAGGPGTLPRLLAGLAAALLLGTAVPGLAATPAASPYAEAAA
                     GLAAKGGWAEAEELAAEGLSYDGADSDLLYLWALAALKSQDDAAASLAALEAALLSGR
                     FSMFEAGAARLLRAELLVRTRRFEKALAALDELERAPAAASASFPSRASDALRLRALA
                     LYGLGRRPEAVAALQAAARRHPDDPRFARLFFERGEAGAAGSGGPAGAAPSGASKAEA
                     DLAAAFLGRLGDRGGKPGLSSLDPELLLLSVPWMAGAEARRDAVAAYRAAGGRGLRST
                     ALALEYGLLSEEAATAEAFAGSEPLSPGFLERLEALVGTEKGRAALSAALRRYSGPFA
                     ADRDGDGQREETALYRDGRLASFRLDADQDGRPDRLVSFRDGLPAEALLAGKDYSLRL
                     AYGAYPAVESLRFEALPPDPGVPGRGLPPKDPSRIREYSFAPEAFAFPLLSFLPFPSP
                     LDDGLYLPLPLDPPEPTERAAASAAYLLRVLGPGEEIRVSLDGGTPLRLDRLRDGRLY
                     SSLPFERGLPLVESLDADGDGRFETERGRDAPLPPGARPFLDAEGAYEEGAVSWTRVD
                     ADGDGLFEYREELRFPYRKEWDLDSDGLADAAQYALAGGGLVREFSSRLDGEFDERLE
                     LDASGSIRSFLRNGARPALVPDKNPRLRWIGSKPFDLGSNLPPEEGVYRYMKQRYRLV
                     YAGPEAFAELLP"
     gene            complement(26695..28572)
                     /gene="ftsH"
                     /locus_tag="PLG14_00100"
     CDS             complement(26695..28572)
                     /gene="ftsH"
                     /locus_tag="PLG14_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOX47555.1"
                     /translation="MDFDDQNPQPPRKSEGARPEGPNRGSRAALAVFLSLGVLFSALF
                     FANDRSSAPEIPYSAFLAHLDQGEVEAVKILDSSEIQLSLRGKAGAEALRRTTIPYED
                     PELLKALREKDVKVSGGFKGVSPWQLLAELLPWLFGFFILWMMLRQVQGNNKAFSFGK
                     SRAKRYVEPAKKITFDDVAGQKEAKYELEEVVDYLKNPQKFVKMGARIPKGVLLVGMP
                     GTGKTLLAKAVAGEANVPFFHMSGSDFVEMFVGVGASRVRDLFDQGRRSAPCIIFIDE
                     LDAVGRTRGAGYGGGHDEREQTLNQMLVEMDGFDTKDGVIILAATNRPDVLDPALLRP
                     GRFDRQVVVAMPDVAEREAILGIHMKKIPVDASVDSRLVARSTPGTSGADLANLVNEA
                     ALFAIRKDRTTVLMEDFEEARDKILMGAARKSLVITDAEKRATAYHEAGHALTLYYLE
                     HSDPLHKVTVVPRGRALGLALALPEKDAYSHTKSWLEDYMVFAFGGYAAEKIVYGETT
                     TGTAQDLRQATDFARKMVCEWGMAEAMGPVSYGQEDEPIFIGKEIARHKDYSEDTAMR
                     IDGSVRDFLEKAAGRAVSILTEHKDQLERLAEELIVKETLLDEDIRNLFGFPPRKAQE
                     A"
     gene            complement(28575..30059)
                     /gene="tilS"
                     /locus_tag="PLG14_00105"
     CDS             complement(28575..30059)
                     /gene="tilS"
                     /locus_tag="PLG14_00105"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOX47556.1"
                     /translation="MQASLDPKAFEARVEGFLSSRGLSKAEPALVALSGGPDSTALLA
                     ALAALGAGALRAVHVDHGLRPRAELDAERDFVRGLCRGLGVPLTIARLRPGAVLERAR
                     ADGEGVEAAARRFRYAAFRAILAREGLSRLYLAHNRDDQLETILMRFLAGSGPGGLRG
                     MPEAAGPILRPLLSTPKSEILAYLAARGLGHSQDSTNDGDAYLRNRVRRGLVPVLDER
                     FPGWGRGLLRGAELAALDEEALAAWAVRVGFAPAPAASSSGAGPGGAATPRAPGSSAA
                     GRLEADAASFFGAPEAVRRRALLQAMSLLGPARPGRRGERQPEGRRLPARLARAALLA
                     ASSGGRYRGAGIELRLEEGRLILSRSLDFPKRRGYFVVIERPGPGGAEIHAGSVTVAA
                     RWSEGPDAAGLDEGAFTFPLVARSRRPGDALSLRRGRKPLDELLSEWGLPAEARGSLT
                     VVEDRDGIVAVLGSAWGGRDRFRHCGGGLGNGRKLSIAVKGALA"
     gene            complement(30151..30840)
                     /locus_tag="PLG14_00110"
     CDS             complement(30151..30840)
                     /locus_tag="PLG14_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOX47557.1"
                     /translation="MNHPVLNAALREGTTKGELRGLRVNGKIPAVIYGGGLAATQVTL
                     DALEFKKATAGISESTIITINIGKDKKEAFVKERQRDALTTEIIHVDFLEVLQDRSLH
                     AKVPVHLVGTPVGVTDGGILENPAHEIEVECFPRDLPEKIDVDVSGLHANHSIHVRDI
                     PAPKGVKILTKGDLVVALVKFAKVEVEAAPAAEAAAPAAGAAPAAGAAPAAGAAPAAG
                     AAPAAKKEEKK"
     gene            complement(30890..30961)
                     /locus_tag="PLG14_00115"
     tRNA            complement(30890..30961)
                     /locus_tag="PLG14_00115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30927..30929),aa:Gln,seq:ttg)
     gene            complement(30991..31275)
                     /gene="spoVG"
                     /locus_tag="PLG14_00120"
     CDS             complement(30991..31275)
                     /gene="spoVG"
                     /locus_tag="PLG14_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOX47558.1"
                     /translation="MEITDIRIRRVTTESKLKAYVTVTFDDCFVVHNVKIIEGKTGIF
                     IAMPSRKTRSGEYKDIAHPITPEFRNRLQERVLKTFESGPHPEATPADFD"
     gene            complement(31320..32216)
                     /gene="ispE"
                     /locus_tag="PLG14_00125"
     CDS             complement(31320..32216)
                     /gene="ispE"
                     /locus_tag="PLG14_00125"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOX47559.1"
                     /translation="MEKTVLVRAPAKVNLHLRVYGRRADGFHGLRSVFQAISLADEIV
                     VGSLTSPDRIEIDGTFDCPPEATTVHKAVRLYREATGSSRGVRIRVGKGIPAGAGMGG
                     GSSDGAAVLKALDALFGDADGRLGEETLLSLGARIGSDVPFFLRGAAAVVEGRGEVVS
                     PFPGREDFCLVVAFPGFPVSTQAAFALLDRERPDDSGEADPEASELLAAYRREPSSWP
                     FANSFEPYVAGAHPAIASVSASLRRSGSPFVRMSGSGSTVFGVFPDPESAGFAAEALK
                     AGLPGGSLVALTLPLACVPSLE"
     gene            32421..32738
                     /locus_tag="PLG14_00130"
     CDS             32421..32738
                     /locus_tag="PLG14_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47560.1"
                     /translation="MVPTYGETAMDPALEPALAPFQGIAPAYPVARFDDGFDEYEDDD
                     FEEDEDFDDLDDEDLDDEDEDFDDDDDFDDDSGDGFEDEDEGDEEEEMDYEDDVDYDD
                     FDE"
     gene            complement(32923..33294)
                     /gene="rsfS"
                     /locus_tag="PLG14_00135"
     CDS             complement(32923..33294)
                     /gene="rsfS"
                     /locus_tag="PLG14_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOX47561.1"
                     /translation="MPNSRESAFSIARTLAEHKGGEVLVLDLAAESGWTDFFVIATAM
                     SSTHLRGLARYADEWASQNKVQRLNTPKIQDDEEWVLLDLGDVVVHLMTERARTFYEL
                     EKLWFKAASTSVEAPAAPGPA"
     gene            complement(33284..34459)
                     /locus_tag="PLG14_00140"
     CDS             complement(33284..34459)
                     /locus_tag="PLG14_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOX47562.1"
                     /translation="MKPVTLDKSSVLLFVILAFLAGTLVFLGLSLRSDAAEEAVKEDR
                     ILNVLFVLELEGKPVATELCLMYPSTGKGALLDVPGETGLILKSLNRVDRIDAVYDPR
                     RPKTFVDEVSNLLDAEVPYWIVLDEKSLSSLVDLLEGVELFIPQPLASAGPPRVLLPA
                     GALVLDGDKLVQYASYRDAEEGEGEAAARRQKLLQSLLRRIGEKASWLERPEAFQAFN
                     RLLRTNLSADLSRRFAAELGKLDADRLVLQRVTGVFRQVDGKRLLFPHYDGELVKDIV
                     KQTLNALSSSGADAVADKIFTVEILNGTPGKGLAKRTAEIFQSFGYDVVSVGNADRED
                     YGSTLVIDRFSNPEAAKNVADVIRCSSVSAQAGMPGETVADFTVILGKDFNGRYCAK"
     gene            complement(34456..35037)
                     /gene="yqeK"
                     /locus_tag="PLG14_00145"
     CDS             complement(34456..35037)
                     /gene="yqeK"
                     /locus_tag="PLG14_00145"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOX47563.1"
                     /translation="MEPARLAALLEERLDAELSPARAAHSRGVAALAASLCARAGLDP
                     ERGRAAGLAHDLCKELSPERQAALAAASGYALESGFLSEKILHGPAAAELLREDYGVE
                     DAELLASVAWHTLGRAGMGLLETFVYCADKIEPGRRDVDPAFRARALSLPPEEMLAAV
                     VENTMAYLSSRGRAVAPETLNLYNTLRDSVQQT"
     gene            complement(35024..35599)
                     /gene="nadD"
                     /locus_tag="PLG14_00150"
     CDS             complement(35024..35599)
                     /gene="nadD"
                     /locus_tag="PLG14_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOX47564.1"
                     /translation="MRLLLLGGSFNPVHWGHLVLAEELREEFSYDLVLLVPAARPPHK
                     ELPSGGASPEERLEMLALAVAGNPCLAVDGCELERPGASYTIDTVRGLKARYPLEGKP
                     GLALGDDLAPGFKDWREPEALAREADLVVARRGGGAFDLPYPCRLASNRLIPLSSSEL
                     RERAARGRSLRYLVPEAVREYIYAKGLYGAR"
     gene            complement(35596..36795)
                     /gene="obgE"
                     /locus_tag="PLG14_00155"
     CDS             complement(35596..36795)
                     /gene="obgE"
                     /locus_tag="PLG14_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOX47565.1"
                     /translation="MIKFADEASITVSSGKGGDGCVAFRREKYIPMGGPAGGDGGRGG
                     DVVFEVRRNLRTLAHLRFQQHFKAGNGQPGMGKNMHGRKGDDVVIPVPPGTLIKNAET
                     GEVLKDFGTETAVDGGGRLGGAKSAARAGEGEGRWLFLKGGNGGWGNTHFKSSVNQAP
                     RQFQPGKPAETVNLRVELQLIADIGFVGFPNAGKSSLLDFFTNARPKIAPYPFTTKIP
                     NLGMLTVHERDIVLADIPGIIEGASDGAGLGIRFLKHISRTAALAFLVDLSDENWREA
                     FGILMDELSSFSPELAKKRRLLVATKLDLPDAAERFDEFKAAFPEESVYGISVFSGAG
                     LPELKETFLRLVLENEAEAEARAAEASRLFAEGGPGFGEATGGAAGALDEEGADADLF
                     GEAGEGE"
     gene            complement(36931..37200)
                     /gene="rpmA"
                     /locus_tag="PLG14_00160"
     CDS             complement(36931..37200)
                     /gene="rpmA"
                     /locus_tag="PLG14_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOX47566.1"
                     /translation="MPHKGVNGRDSNPQHLGVKAYGNQVVTAGSIIVRQRGTKIHPGL
                     NVGCGKDDTLFALVDGRVFFKEFKGKKYAGVEVLAETASDAPAKA"
     gene            complement(37202..37543)
                     /locus_tag="PLG14_00165"
     CDS             complement(37202..37543)
                     /locus_tag="PLG14_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOX47567.1"
                     /translation="MIAAKAVLDAEGRLVRFEAEGHSGRGPKGRDIVCAAFSVLARSA
                     YEALSALPGAELEGSAREPGSLSFRVRSLGAGAEGKAAGIADFLLAGISGLEREFPGE
                     VGLTIERYWRE"
     gene            complement(37603..37911)
                     /gene="rplU"
                     /locus_tag="PLG14_00170"
     CDS             complement(37603..37911)
                     /gene="rplU"
                     /locus_tag="PLG14_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOX47568.1"
                     /translation="MYAVVEIAGKQYRAEKGATLRVDRLEAEKGSALSLDKVLLLSGD
                     TVKVGSPYVAGAAVKAVVEDEVKGDKIVVFKYKPKKDYRRTQGHRQSYTLLKVEEIVG
                     "
     gene            complement(37984..39747)
                     /gene="argS"
                     /locus_tag="PLG14_00175"
     CDS             complement(37984..39747)
                     /gene="argS"
                     /locus_tag="PLG14_00175"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOX47569.1"
                     /translation="MVEAKTAWKAIVARALAAYIAEKGSPAEPPSPESVVLERPPKPE
                     LGDFGFPMFPYAKLLRAGPPMLAQALAAIVAKDPEAQALGTAVAEGPYLNVRLSRAPE
                     AARILAEAGQASWGATLSLSGRKVMVEFSSPNTNKPLHLGHLRNNVLGESLSRVLSAA
                     GAEVRKINHVNDRGVHICKSMLAYMRLGGGRSPEDEGLKSDHFVGKYYVLFNELKAKD
                     PKVEEEAQELLLKWESGDPETRELWKKMNGWAVGGIKATYARTGISFDLFDFESETYE
                     LGRDEVLRGLAAGVFYKEEDGSVWVNLEDIGLDRKVLLRKDGTSLYITQDIGTAISRH
                     ESWPYDRLVYVVGNEQQYHFKVLFEVLRRLGFGWASDLHHLSYGMVNLPSGRMKTREG
                     TVVDADDLVDELSALAAKEIEAKGREGAVGDAAQVGERIALGALHYYLLQTAPTKDML
                     YDPEASLSFTGDTGPYIQYMGARASSIIRKHEAGEGNAAKGSPEAGRLSGDADWALLK
                     LVAAYPEALDQAAEAMDPSVVAAYLHELAVGFSAWYRDNQVLNCPDAGLAASRLELVR
                     AVRRTLEAGCRLVCVPFLETM"
     gene            complement(39912..40823)
                     /locus_tag="PLG14_00180"
     CDS             complement(39912..40823)
                     /locus_tag="PLG14_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47570.1"
                     /translation="MNDQLDAFKPLLYGLADASAFLFTTVPGLLLLGGLVLAAAVASV
                     AKALSERRLLAAAAGRNLRAGEAAGIALSRLAAFAAELFTRLPLLAGLAVVLVLASAA
                     AGSLKGLDEYVANRERIAELQAAVRNLDRRQRVADVAILESGDGRFTAEFSFYEGGRA
                     RPREASQRVELTGRELYVDAVVCNFAYSEITEGRAVNLALPYRAFSEAVPAAEGEALD
                     LLDSGGVPLMYRLPPEEIYGLEPDRYAERLGEIAALLRDEKAARGAGVVRSLYGAALH
                     RPVRRGDRFTIWAEQTGGLTIKDASAF"
     gene            complement(40826..41530)
                     /locus_tag="PLG14_00185"
     CDS             complement(40826..41530)
                     /locus_tag="PLG14_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47571.1"
                     /translation="MKKPLRLLAWLLALVLLAALGAWLVLGLGTARQREKIAALESLE
                     ARLTAERVPLRFMVLSRSSDEISARVKLYDLDGKELAALERSFRGGELFFDFVVAPLG
                     EDWLAFPRRVFTELTPAQEGVSLLGYYEREGFPGVFGSGSMGKAEREAVKELYREALR
                     AAGGLEPGEEGQAGGTPGGGPAGGRRTGGSEAARLAERSFGSAVHEVSTLSRFDVGVV
                     YKIVCRAKGGIEIMED"
     gene            complement(41527..43440)
                     /gene="ligA"
                     /locus_tag="PLG14_00190"
     CDS             complement(41527..43440)
                     /gene="ligA"
                     /locus_tag="PLG14_00190"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOX47572.1"
                     /translation="MPKKKDRIAELAGLVKRHQDLYYNGQPEISDAEFDALWDELADL
                     DPDNPVLGAVGADSADGWPKAAHLIPMGSQEKASNPEDFLAWCAKVGHPEYLVQHKLD
                     GASLELQYEEGALVRAVTRGDGAVGDDITPNARRMRGVVERLPEAFTGGVRGEVLMFR
                     AVHDAKYADKANCRNAANGLMKRKDGSGSGDLDVICYDAAGTVPSGGDLFGSERASPF
                     ADEVEKLAWLSRMGFETVPLVRCGSGEEVVEHRARVMAERPSLPYDIDGLVVKAREID
                     EADLARARPVRQIAFKFSLEEAVTSLLSVEWSESGASYTPIALVEPVRLAGTTVQRAN
                     LCNTNIISGLGLRVGSRVVITKRGEIIPKIESLVENPPGTKAIEVPSACACGSALVDE
                     GTRLYCPNPECPKKALHRLEKWLSVLDVRDFGSVILGKLHAAGRVREIADLYSLTAEE
                     LALYDRMGETLARKILRNLAAKAELRLSEFVAGFDIEGIGETMAEKAAKAGFDSLEKL
                     RAATVEELDEVEGFAEITARALVEGLAALRPEMDRLLATGAVRVAAPASGGPLAGKSF
                     CFTGELKAMKRPEAEALVKSLGGEAKSSVTKGLSYLVTNDPASGSDKNRKAQAYGVEI
                     IGEEAFLALAGRA"
     gene            43733..44956
                     /locus_tag="PLG14_00195"
     CDS             43733..44956
                     /locus_tag="PLG14_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47573.1"
                     /translation="MNTSPAPDRRMLLLLPSAFLTSLGIGVINLGMLFVVKDEYRASP
                     SAVGWFAALWAITYFLGCVALRPLHQRIGARLSMAAMNLGTALLLLAHLFTRNLASAF
                     AVYGLYGFITALFWPRLMGWLSSGLEGQALSKATSAFSFSWSSGGIVAPYLAGLLAEK
                     GRFLPVYVSIGVFAATGLFIALSSRLVATPPFAPTPLAPAAKGSGGQTGAEAEGGARA
                     EAATTADRSTPLRFPAWIGVFLLYALLSVFFNIFPLFAKDELGLSESRIGLILLIRAV
                     STTAGFWILGRVETWHFKKAYIVAPIAIALLIDLAFIGVRSPLLFGVLLAVAGFFHAM
                     AYNNSIFYGVSGALDRDKRMTIHEALLTAGQILGSLAGGMLYQRFSWGSIFLFLAFVF
                     VAGMAGQLRYLRRRS"
     gene            44956..46728
                     /locus_tag="PLG14_00200"
     CDS             44956..46728
                     /locus_tag="PLG14_00200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOX47574.1"
                     /translation="MARAGSPAARPSARLGLLALSLLASAFLFLSSCSPRERLLRLET
                     ASEEAAVLKGHLADHPLPKGWRLVGPAAAGDGAAAASGAAAGVAADAVLSLAWVRVAH
                     SEAGDEAGSRGVSAAGRPSLGRRYLAAAAGLAEPVYSLGSLEAEARGLEPLEGIRLPR
                     RALAVEGLWPGEEGYPYEEELILSIGPAAEPAAERTAAAAQTAAAERTAATAPSAAVA
                     PAAGPAAAPKAAYKVPRELGRWLSRLAAQAESPTAGGSPLPLDTAPRGPATPGRGRAE
                     RPFTIGAVGDLAIADSDAPRLSGGPSGLASLFGSALPALSRPDLLVGNLEGVVSPLGE
                     PNPRKRFRFRFPPETPRALASAGFDLFLFANNHGFDYGSEAFTDSLAALEAAGTPFVG
                     AGRSLAEAARPWRAALPAAPGRGLAFIGFARYPDERYGFTAEEAAAGPSKAGVLSDES
                     EALRAIGEAAAAGDFVVVLAHGGEEYRGVPSRPTRELYRRFVDAGADLVLGSHPHVLQ
                     GAEAYSGGLIAYSLGNFLFTADKEPPEALPSAMLEFLVEGGEIRGVRLEPVLALEEGT
                     RLDPDRTGAEARFAALCAGLAASP"
     gene            complement(46877..>48176)
                     /locus_tag="PLG14_00205"
     CDS             complement(46877..>48176)
                     /locus_tag="PLG14_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX47575.1"
                     /translation="SAAPADVALLLKPAEPPLPEKAETASVVEPTTQKAPAAKTLAPE
                     VPEPALAKAEPAAPAAAPVVPQATVAAAPAAEAKAPLEPAPALPVATPPSAAPTARAE
                     PSVLPEAIVRVDARPETAASPELALPDPEERGAAALVGAPEEGPAVLGLEAAALPPGD
                     GALAALDEPELRPDELPEESLARLENPPASPLDFALAEGEIDMAEREKAEALALEKPA
                     ALESSLQAELAEAEVPSEAEAEPLDVAEASEEALGPLPAGEASEVALAEPEPSAEDEA
                     EVEVVAAAPTAPAAEPEAEVDLALSLEPTASRPPVQPAAPVQPAAPAQVATVPAKPAP
                     VAASVPSGPLTPPAGLPLLARLAKGSFYIQIGVYGTNEALHAAVKGFKGDYPLALEEA
                     ASGGARYRLYVGPLSRDEGGVVLSRVKAMGFKDAFIKQGT"
BASE COUNT         6896 a        16584 c        17541 g         7155 t
ORIGIN      
        1 ggcgagcggc ccttcgacac ccagatcatg ggcggcatcg tcctccacca gggcaggatc
       61 gtcgagatga agaccggcga gggcaagacc ctctgctccg tcggcgcggc ctacctgaac
      121 gccctctccg gcgacggcgt ccacgtcgtc acggtcaacg actacctcgc cgagcgcgac
      181 tcgcagtgga tgggccaggt cttccgcttc ctgggcgtct ccgtcggctg cgtcctgtcc
      241 aagatggaca acgaggcccg cgtcgccgcc taccgcgccg acatcaccta cggcaccaac
      301 aacgagttcg gcttcgacta cctccgcgac aacatgacct ggagccccga gggcagggtc
      361 cagcgcggcc acaactactg cgtcgtcgac gagatcgact cgatcctcat cgacgaggcg
      421 cgcacgcccc tcatcatctc cggcccggcc gacgacgaca ctttcaaggt aaacgaggtc
      481 ggcaagctcg cctcccagct cgtcgaggtc aagaaggatc ccgaaaccgg cgagtacccg
      541 aaggaggagg agggagaagt cctggagggg gactacaagg tcgacgagaa gtcgaagcgg
      601 atctccttca cctccgacgg catgaccaag atggagggcc tcctccagaa gcgcggcctc
      661 atcaagggct ccctcttcga cgagggcaac ttcgagttcg tccattactt cacccaggcg
      721 gcccgcgccc agatcctctt ccagaaggac gtcgactacg tcgtccagga gggcctggtc
      781 cagatcgtcg acgagttcac gggccgcatc ctccacggcc gccgctactc cgacggcctc
      841 cacgaggcca tcgaggcgaa ggagaggatc aagatcgcga ggcggaaccg gaccctcgcg
      901 acgatcacct tccagaacta cttcaggatg tacaaaaaaa tatccggcat gacgggcacg
      961 gccgacaccg aggcgccgga attccagaaa atatacaacc tcgacgtctc cgtcatcccg
     1021 acgaaccgcc ccgtggcccg cgccgacgag gacgacctcg tcttcctgaa cgaggcggag
     1081 aagttcgagg ccatctgcga cgagatagcg gaggcccaca agaagggcca gcccatgctc
     1141 gtgggcacgg tctccatcga gaagtccgag cacctctcct ccctcctgac caggaggggc
     1201 gtccgccacg aggtcctcaa cgccaagaac cacgcccggg aggccctcat catcgcggag
     1261 gccggagcca agggctccgt caccatcgcg acgaacatgg ccggccgcgg cacggacatc
     1321 aagctcggcg gcaaccccga attccgcgcc cgccgccgcg ccggcagcca ggcctcagac
     1381 gaggagtaca aggccgccct ggccctcgag tacgaggcct ggaggaagga ctacgaggag
     1441 gtgcgctccc tgggcggcct ctacgtcctg ggcacggagc gccacgagtc gcggcgcatc
     1501 gacaaccagc tccgcggccg ctcgggccgc cagggcgacc cgggccgctc ggccttcttc
     1561 ctctcgctgg acgacgacct catgcgcctc ttcggcggcg agaacctgaa aggcctcatg
     1621 tcgaagatcg gcatgaagcc cggcgagccg atctaccatc ccctcctgaa ccgcacgatc
     1681 gagaacgccc agaagaaggt cgaggagcgc aacttcgaga taaggaagca cctcctcgag
     1741 tacgacgacg tcctctccga gcagcgcaag ttcatctacg agcagcgcga cgccatcctc
     1801 aaggagaccg acctcctctc gcgcgtcagg gactccgcct ccgacatgct cgacagcttc
     1861 ctggaggcct tctccgacgc gcagaggaag gaccagggca gggccctctc cgagctccag
     1921 acggccctga aggacgcctt caacgtccag ctccccttcc gcgccgacga gctttcggga
     1981 aaggccaggg aaggcctcaa ggcctcgatc tccgagctcc tggagcgcga cctgtccggg
     2041 aagctcgagt acgcgggccc cgagaacctg aaccacttca tccgcttcca gtacctccag
     2101 gccatcgacc agaagtggct ggaccacctg gagcagatgg aggccctgcg cgaggccgtg
     2161 tacctgcgct cctacgcgca gaagaacccc ctcctcgaat acaagctcga gggcttcgac
     2221 atcttcaacg agatgatcga ggagatccgg cgctccatcg cgtcgcgggt ctacctggtc
     2281 cgcatccaga ccgcggagga gcgcagggcc cgccccgtct ccgagcgcgc cggcaacgcc
     2341 gtccacgagg acgccgccag cttcgccggc gcgggctccg gcgcgaagag cgccgtggcc
     2401 gaggccgcgc ggcccgcggg cgcgacggtc gtgcgcggaa ccgagaaggt cggccgcaac
     2461 gatccttgcc cctgcggctc gggcaagaag tacaagcact gccacggcca gtgaggccat
     2521 gtcgctcaac catcgggaaa tcgacctggt cctcgcagag ctggacctgg agggagccca
     2581 gctccagcgc gtggtccagc cctcctacga ctcggtggtc ctgggcttct acaagtcggg
     2641 gacggagagc gagctcctca tctccatagc ccacggcgcc tgcaggatcc acaccctgtc
     2701 ctcgcgcccg cccaagcccc agcggcccct gcgcttcatg gagtgcctgc gctccaggct
     2761 ccgcggcggc cgcctcgagt ccatagtcca gctgggcgac gagcgcatcc tacgcctgga
     2821 cttcctggtc gtccgcaagg agggcgccga ggaggaaggc gagcgcagct accggcccat
     2881 cggggcctac gcgcgcgacc tcgcgcacgg ctcggcgcgc gtcgcgattc acggcgcgac
     2941 ggatggcgcg gcgcaggcag cggcgcaagg cgccgcgcgg gacgaggcag gcccggccga
     3001 atccgacggc ggagaagcgg ctcccgaagg cggcacggcg cggcgctacc gcctctacgc
     3061 ccggctctgg agcggagcgg gcaacctcat cctctgcgag gaggacggga ccatcgtcga
     3121 cgtgctcgcg cggcggccca agcgcggcga gatctcaggc gagtgttgcg cgatcgaaga
     3181 ggagctcgcc gcggccaggg tcgcgtccgg ggccggggac aaaggcgcgg ccaggggggc
     3241 cgagggcgcg gccggagcct cggggccggc gcggccggcg cggaccttcg agccccggga
     3301 cttcccgccc gcgccccgcc gcgaggacgg cgggagctac aacgagcgga tcgaggcctt
     3361 ctacgcctcc cagggcggcg agctctcccg cgagcggctc ctcgaggccg cgagggagcg
     3421 cttttccaag cgggagcggg ccatcggcgc gcggctcgcg gagctcgagg cccgggctgc
     3481 cgagtaccgc ggcggcgagc gcttccgcga actcggcgac atcctcatgg cgaaccagtc
     3541 cgcggcgccc gagggccgat tcctgccctg cgaggacttc taccgcggcg gcatggtcga
     3601 catccccctc gatccgcgga agagcgtcgt ggagaacgcc caggtcttct acgagcgctt
     3661 caggaaggcc ggctcagggc tctcggagct cgagtcggag atagcctccg cgaaggcctc
     3721 cctcgccgag gccaaggccg agctcgagag gctcgagtcc ctcaaggagc ctctcctcat
     3781 cgcccgcgcc ctgcagaagg gcggcacggc ccgcgagccg aaaaagcggc cctaccccgg
     3841 cctctccctg gagcgcgacg gctggaccat cctggtcggg cgctccgcga aggagaacga
     3901 cgagctcctc cgccgccacg tccgcggctc ggacctctgg ctccacgccc gcgactactc
     3961 aggctcctat gtcttcgtca aggcgcgggc gggcaagagc ttccccctcg agatcatgct
     4021 cgacgcgggc aacctcgcga tctactactc caagggcagg gccaacggcg gcggcgagct
     4081 ctactacacc caggccaagt acctgaggcg cgccaaggac ggccccaagg gcctcgtgat
     4141 cccgacccag gagaagaacc tccgcgtgac cctggacgag aagcggctgc gcgagctcaa
     4201 ggagctcatc ggcgacgagg gcgactagcg cctctccgtc tcgcagcggt tcttgccggc
     4261 ggccttggcc cggtagagcg ccgagtccgc gcgctccagg atcgtgaggg tcgagtcgac
     4321 ccctccgccc agggaggcga ggccggcgct gatggtcagc cttcgtccga ggccgggcgt
     4381 cctcgcctcc agtcccgact cgacggccag cctgaggcgc tcgcaggcgg ccagggccgc
     4441 gccgaggggc gaggatggga agaggatgag gaattcctcg ccgccgtagc gccccacgac
     4501 gtcgtagggc cgcagggtct ccttcatgac ggccgcgagc gagaccaggg ccgcgtcccc
     4561 catctggtgg ccgagctcgt cgttgatccg cttgaagttg tccgcgtcga tgatcgcgca
     4621 ggagaagggc gcgcccgagc gcttggcctc cgcgaccgcc tcgtcgaagc gggccaggac
     4681 gtacttccgg ttgtggagcc ctgtgagggc gtcggtcacc gccgcctctc gcagctccga
     4741 gcgcgccttc tccagcttca gccggaagcc ccgcatgagg aggtggaaga cgccgagcat
     4801 gaggacggtc acgagaaggc cggtccccgc gatggccagg taggtgcggc gcttctcccg
     4861 gtcgatggcg gagaccggga aggacacgga gatgccgccg cgcacgtccc cggccttgta
     4921 gccctgggcg gcgtggcagg cgaggcagga cgcgtcggcc ttgaggacgc ccatgtagcg
     4981 gaaccaggcg ccgtccgggc gcgcctccct ggcccaggtc tccgaccctc cccgctcaaa
     5041 gccctccagg gcggccttct cgaagggatc gggggcgttc gaggggttca cgggccttag
     5101 gctcgttatg cgaaagcgga agctggccac gttctccgcg agctcggaga tctcccgggt
     5161 catgagggcg ggattccgct tggtgaggac ccggccgtcc gcggcggcta tgtcgggatc
     5221 tgcgagccag gggttggact cgacgccggg acctttcagg acatagacgc ctccgcgcag
     5281 ggagttccac ttcctggtga ggactatctc ctcgaagagg gacctggcgc gggccaggac
     5341 ggtctcgcgc accagctcct cgttcctgag ggccatgccc gcgaagaggc cggccaggca
     5401 gagcacgagg gccgcgttga aggacatcgc aaggatgagg taggcccggc gctcggccgg
     5461 gtcctcgaag gccatgcgcc ggcccttgat gcgcctcatg cggccgccgg ctagccgcgg
     5521 cccttgaggg ccggctcgcg cttcgggtcg ggcttgggcc gcacgcggat cgcgccctcg
     5581 cggagctcga ccttggccac ggagccgggc ccgagtcggc ccgcgaggat ctcctccgcg
     5641 agtcggtcct ctatctccga ctggaggagg cggcgcatgg gccgcgcgcc gtaggcgggc
     5701 tcgtagccct tctccgcgag ccagtccttg gccgaggggc tcacctcgag gacgatgccc
     5761 ttcagggcca cgcgccggga gagttccccg aggaggagct cgaggacctt gcgcacctcg
     5821 tcccgggaga ggggcttgaa gaccacgatg tcgtccacgc ggttcacgaa ctcgggattg
     5881 aagcggcgct tgagctcgga gagggaggag gagcgaacct cggcgtagga gaggagcctg
     5941 tcctcaggcc tgaagccgag gccgcccgag gagatctccc gcgcgcccgc gttgctcgtc
     6001 atgatgacga cggcgttccg gaaggagacg gcgtggccca ggttgtccct gagctcgccc
     6061 tcctccagga gctggaggag gatgttaaag acctcggggt gggccttctc gatctcgtcg
     6121 aagaggatga ccgagtaggg cctgcgccgc accttctcgg tgagcatgcc gccctcctcg
     6181 tagccgacgt agcccggcgg cgagcccacg aggcgggccg cgttgtggcg ctccatgaag
     6241 tcggacatgt cgatgcggat cagggcctcc tcggagccga agaggatctc cgcgaggcac
     6301 ttcgcgacca gggtcttccc gacgcccgtt ggcccgagga agatgaagga gcccaggggc
     6361 cgcttcccgt cggagacgcc ggcccgcgag cggcgcacgg aggaggagac ggccgcgatg
     6421 gcctcctcct gtcccacgac gcggccgtgg agggcctcct cgagcctgag gaggcgctcg
     6481 gcctcgttct ccgcgagccg ccccaggggg atgcccgtcg cctccgagag gacgaggcgg
     6541 aggtcgtcct cggtgacggg cgcggggccg ccgcggccgc ccgactccca ctcggccttg
     6601 agggcctcga ggcgcgcgcg gaggccgcgg acgcggtcgc gggtctccgc ggccttctcg
     6661 tagttctgcg tggagaccag ggcgagcttc tcctcggtga ggcggcggat ctcctcctcg
     6721 atctccgaaa gctcgggagg cctcgagggg ttggtcacct tcttgagcgc ccccgcctcg
     6781 tccaggaggt cgatcgcctt gtcggggagg aagcggtcgg cgaggtagcg cttggacagg
     6841 tccacggcgg cctccacggc gccccgcgag taggcgactc cgtggtactc ctcgtagcgg
     6901 gacttgattc cctccagcat ctcgagggtc tccgactggc tcggctcctc cacgaggacg
     6961 gcctggaagc ggcgctccag ggcggcgtcc ttctcgaagt acttgcggta ctcgtcgagc
     7021 gtggtcgcgc cgatgcactg gatctcgccc cgggagaggg cgggcttgag catgttggag
     7081 gcgtcgatgg agccctccgc cccgcccgcg ccgatgacgg tgtggagctc gtcgatgaag
     7141 aggatgacgt tgcccgcgag ggctatctcc ttcatgatgc gcttcaggcg ctcctcgaac
     7201 tcgccgcggt acttcgtgcc cgcgacgacg gaggcgatgt ccagggcgag gaggcgcttg
     7261 cgcgagagct cgtccggcgc gtcgccgcgg gcgaagcgca gggcgaggcc ctcgacgatg
     7321 gccgtcttgc cgacccccgg ctcgccgacg aggacggggt tgttcttggt ccggcgcgcg
     7381 aggatccgca cgacgcggcc gatctcgcgc tcgcggccga cgacggggtc gagccggccc
     7441 tcgcgggcca gggcggtcag gtcgcgggcg tgctcgtcga ggaggggcgt cctcgcgggc
     7501 gcctggcccc gcggcgccgg ggccggggcc ttgcgctcct cggggggacg gcgcgcgtcg
     7561 gcccgctcct ccgcgccctg gcccggctcg cggaggaggc ggacggcggc gcggagctgc
     7621 tcgtagtaga cgccgtagcg ggagaggaag cgctcgaagg cggcgccctg ctcccgggcc
     7681 gcggcgacga cgaagtgctc ggtcccgatg tactcggagg aggacatgtt cgcctcctcc
     7741 gccgccgtct cgaggaggct ccgggagcgg cgcgagagcg ggaggtcgcc gaggacgaag
     7801 ccggcgcgct tgcgctccag ggagcgctcg agctcgtcgc ggagctcgag gggatcgatc
     7861 ttgaggctgc ggagggcctt gaagcccaag ccctcgccct ccctgaggat cgccaggagg
     7921 acgtgctcgg gctggagctg gtccgagcgg ctccgcttgg cctcctcctg ggcgagggtc
     7981 gtgaggacgc gctgggcgcg gtgcgttagc cccttgaaca tcgatctcct ccctagctca
     8041 atactaatgc tgcgactgcc ctctcggcca gtcgcgcccg ctggcgctcg gcctccgcgg
     8101 cgtcggcgag ggccgaggcc ccgcggagcg cgaggcagcc cggccccagg gagacgagga
     8161 ggccggcgag ggcctccggc gtcgccgccg gcgcagagcc cccggccgcg acggcttgcg
     8221 cgacgccggc ctcggcgacg ccgccctcgg cgacgccgct ctcggcgacg cccgccagga
     8281 gggcgagccg caaggccgag acgtgggcct gggcctcggc ggcggggagc cgccggcagt
     8341 agcggaggag gccgagggcc cgtcccgcga ggtcgaggag gtcctcgcgc cgcgggcccg
     8401 cgagttcggc cctagctcgc cgctccgcct tgacgagggg cctaaggacg gcttctagac
     8461 cttccgcgag ctccgccggg gagaggccgt aggccctctc ggtcgagacc tcgtagaggt
     8521 ctcccgccga gcccgtctcg gcgccataga agcccctcaa gccgaagccg gcagcgagga
     8581 cgggccggaa ggcccgctcg gcgaggccgg cgacggcgag ggccgggagg tggagggtcg
     8641 ccgtgagcgc tacgcccgag cccaggtcgg ccgtcctggc ggaaagatag ccgaactcct
     8701 cgtcgaaggc tggctcgaag cccctcgccc cggccgcggc ctcgaaggcg gcggcccggc
     8761 tcagggccag gcccagcgcg gcctggggcg agaggccggg aaggcgggcg acgaggcgga
     8821 gatggtcgat ctcgtcgaaa aggcagtaga agggttcccg tcccgaatag gccaggaggg
     8881 cgccgggatc gagggcgtag gccctggaga cgaagcccgt ctccacgagg gcgcgccgcg
     8941 ccgcctctcc ggccgaggcc agggagagga ggcgccaatc ctccagggcc gcggcgactg
     9001 gggctgcgat cttgcgcgag ggggccgatt ccgagcccgc gccgggaaag gccaggcctg
     9061 agaggttcct cgcgacgcgg gccttggtcc agaggacgac gtcggcgtcg gagccctccc
     9121 cgcccgagag gacgaagccg ggagcgccgc ggatcagggc gaagggctcg gccgcggcgg
     9181 cgggaaaagc tccgcccgcg gcggcagggc cttcggcggc gctcgggacg gcagggcctg
     9241 cgccggctgg cggagcggcc gtcgcggagg gtgcggccgc cgcggcggtc ggcgggttgg
     9301 cggagccagg ggcggctcct gcggcattca gcttcgcgag gctgtcgcgg atcgaggccg
     9361 cgagttcgta cgcttccgcg gcgagggcag cctccaaaga ggcctcgagg gcgggctccg
     9421 agagccgggc ctcggcaagc tcgcccgtcc tggcttccgg cccgggctca ggcgccggct
     9481 tgtgcgccgg cgcggctcgg accgcggact cggcgccggc cgcgggagcc actccgcgga
     9541 agaagggcgg cttggcggaa gagcggagga tgcgtccgag ctcggcgcgg aaggtggcga
     9601 cgcaggaggg gcagccgaga cggccctcgc gcctgagctc ctcgacgccc aggccgcagg
     9661 agggacaggc cgcgggcagg ccggtccccg cctccgcctc cggcgcgagc agctcctcga
     9721 tgcgaagctc gatccggccg ttgcccgccg agaggcccct ccgcttcgcg cattcctcgc
     9781 agagggccag gtcctcgctc gagtcgccgc ggctccggcg tatgaacagg gcggcttcgc
     9841 gctcgccgca gatggcgcag atcatgtcag ctgactatag tcgaaggcgc ctgaaaagta
     9901 tagattcttc aataaagagc cacgttcaaa ggagtcattc ccatgacgag acgatatgcc
     9961 ttccgcgcct ccgcactggc cctcctggcc ttcggcgccg cctttttcct ccccgcccag
    10021 gaggccggca agggccaggc ggtccgcctt ggcgccctga aggggcccac gggcatcggc
    10081 atgatccatc tcttcgagac gcctcccgag ctcccgggcg gcggaaggct cgaacccgtc
    10141 gccgtcccca acgccgaggt catgacggcc aaggcggtct ccggcgagta cgacttcgcc
    10201 gtccttcccc tcaacatggc ggccaagctc tactcggccg gcattccctt gagcctcgcc
    10261 gccgtcgtgg gcgacgggat ggtctccttc ttgacctcgg acccggccgt ctcctccctc
    10321 gccgacctca agggcaagga aatcttcgtg gccggccagg gcgcgacccc ggactacctc
    10381 cttcgccgcc tcttgaaaga gaccggcctc gatcccgaaa aggacctgcg gctcgcctac
    10441 tccctgccct acccggagat ggccgcggcc ttggcggccg gcaagataag caccgccgtc
    10501 ctccccgagc ccttcgccac cctcgccctc caggccaacc cggccctcaa gagccccctg
    10561 gacctgggtg cgctctggac caaggccacc ggccagaaga gctacccgat gtcggccctg
    10621 gtcgtgagcc gcaagctcgc cgccgagagg ccggaggccg tccgcgccgt cctcgctgcg
    10681 gtgaagtcct ccatcgaggc ggtcaaggcc gatcccgcca gcgccggcct cctcgtggag
    10741 aagcacgaac tcggcctcaa ggctcccgtc gccgcgaagg ccatcccgag gagcgcctat
    10801 gtcttcgtgc cggcgcccgg cgcccgcccc gcggtggagg ccctcctcag ggtcttcctg
    10861 gagacctccc ccgcctccat cggcggcaag ctccccgacg ggggcttcta cgccgccttc
    10921 taggcgccgc gcggcgatag gcgccgcctc gagcgtcggg atactcctcg cctggaagct
    10981 cctttccctc gccgtgggca aggagctcat ccttccgccg cccgagcggg tcttcgccga
    11041 ggccctggtc ctctacccga gcccccgctt cctggcggcc ctcttcggga ccttcctgcg
    11101 gggcctcgcg gccttcggcg tctccgtcct ggcgggagcg gccgccggcc tcgcggccgg
    11161 cctgtccccg ctggtcgagg ccgccctggc ccccctcctc acgctgatcc gggcgacccc
    11221 cgtcctggcc ctcatcctgg tcgccctcct ctggttcccc tcgggcgccg ttccggtctt
    11281 cgccgccttc ctcatggcct tcccggtgat ggcgacgagc gccgcggaag gggcccgagc
    11341 ggccgatccc cgcctcctgg agatggcccg gctcttccgc gtgccgcggg cctcggtctt
    11401 caggaagatc cgcctgcccg ccgccgcccc ccacctcctg gccggcgcga agagcgccct
    11461 cggcctctcc tggaaggtgg tcgtcgccgg cgaagtcctc tcgcagcccc tgcgcgccct
    11521 gggcacgggc atgcaggacg cccgcgtcct cctggagacg cctcgggtgc tggcctgggc
    11581 ggccgcaacg gtcgccctct gcggcctcac cgaatgggcc ttcggccacg ccgcgaagag
    11641 ggcgctccgc catgtcctat gagctccgcg cggcgcgcaa gtcctacgac ggccagcccg
    11701 tcctggacgg cctggacctg tcgatcggga agggcgagat cctcgtcgtc ctggggccct
    11761 cgggctgcgg aaagacaact ctcctcaatg tcctcgcggg cctttcgccc ctggactcgg
    11821 gcctgaggct cggcttcgag gccgcccgct tctcctacgc cttccaggaa ccccgcctcc
    11881 tgccctggct cacggctcgg gagaacgccg ccttcgccct ctcaggcgcc ctgccgcgtg
    11941 aggaggccct gtcccgcgtc gacgccctgc tcgaggcggc gggcctgtcg gcggccgcgc
    12001 agaggcgccc cggcgagcta tcgggcggca tgcgccagcg gctatccctg gtgcgggcct
    12061 tcgcctatcc cgcggagatc ctcctcctgg acgaggcctt cgagtccgtc gacctgaaga
    12121 cgaagatcga gctcatggac gccttcctgg ggctatggaa gagggaaagg ccgacggccg
    12181 tctgcgtcac ccacgacgtg gaggaggcgg cctacctggc cgacagggcc atcgtcctct
    12241 cgcctcggcc cgcccgggtc gtggacgact tcgccatcga ggcgccgcgg gagctccgct
    12301 ccctgggctc ggaagcgacg atggaggccg aggcgaggct ctaccgcctg gtcctgggcg
    12361 cgggccgcta gcccggctct atcgcctggg cgggccgcta gcccggctct atcgcctggg
    12421 cgcgggccgc tagctcggct cggccagggc ctcgtcgacg gcttccgaga gctcctgctc
    12481 cgtgaagggc ttcgggagga agcggcccgg ggggctcccc gccgcggcct tccccgaggc
    12541 cccgtcagcg gccttgtccg tggcgcgctc ggggtggccg gagatgaaga tcaccttgag
    12601 tccgggtatc gcgcccgcga gtcgcctcgc cagcctgtag ccgtccatga tcggcatcac
    12661 gaggtcggtt acgaggagct cgatcgggct gccgccgctc tccgcgagga ggatggcctc
    12721 gcccgcgttc gcggcgacga ggacgcgatg gccgcgcctt ccgaggaact tgccggccag
    12781 gaggcggatc tcctcgtcgt cgtcgacgaa gaggatcgtg cccgggcggc gggccagggc
    12841 ctcgcgctgg tcctcgctgg acgaggccgg gccgccggcc gctcccgcct ggggccgccg
    12901 cgtctcggga aagtagacgg tgaaggtcga gccctcgccc gaggccgagc tcacgccgac
    12961 ggctcctccc gcctgcttcg cgatgccgta gacggtggag aggccgagcc ccgtgccctt
    13021 gtcgcgggcc ttcgtcgtga agaagggctc gaagatccgc ggcaggatct ccggcgcgat
    13081 gccgccgcct gagtccctga ccgagagggc cgcgtagcgg ccgggcgcca gggtctcgag
    13141 gccgaccgcc ctcgggacgg cgagcgactc caggcgcgtg gagacgagga ggcggccgcc
    13201 gccgggcatc gcgtccttcg cgttgacgac gaggttgacg acgacctgct cgacgtgggt
    13261 cgggtcggcg aagatctcgg gcaggccctc gcccaggtcg aaggagaggg agatctcctc
    13321 cgtgaggagg cgcctgagca tccgctccat gtcgcccacg agggcgttga gcgcgaggtt
    13381 cttgggcgcg acgggctgct ggcgcgagaa ggcgagcagc tgccgcgtga gcgccgccgc
    13441 cttcgcggcg gccttcctga tgccctccac gtcgtccttg agctcggggt acttctccgc
    13501 gctctccgag aggaggttcg cgtagccgag gacggccgtg aggatgttgt tgaagtcgtg
    13561 ggcgacgccg ccggccagcc gtccgaccgc ctccatcttc tgcgactggc cgagcctctc
    13621 ctccaggacg cggcggtcgg tcaggtcgga gaactcgccc tggatcccgg cggcgttgcc
    13681 gtcgccgtcc gtgaggaggg ccgcgttcgc gagcacgatg agctcgccgc cgcccggctt
    13741 cctgagcgcg agctcggccg cctccacgcg gcgcgaccgc gcgaggcccg ccttgaagga
    13801 ttcccaggag gccgagtcgt ggaagatctc cctaaggcgg ggcagcggcg cgtccgcctc
    13861 gagcccgatg aggcggcgga aggccgcgtt cgcctcgatg acgcggtcct gcgcgtccgc
    13921 gaggaagttg ccggagattc cctcctggaa gaggcggcgg aagcgctcct cgctgtcgcg
    13981 gaggcgccgc tccatccccg cccggtagag ggctatctcg atggaggtgc ggagctcccg
    14041 ctcctcgaag ggcttgagga tgtaggcgaa gggctgggtg agcttggcgc gggctatcgt
    14101 ctcgtcgtcg gcgaaggccg tgaggaggat gaccggcgtc ttccagcggt cgtggacgat
    14161 cttcgcggtc tccacgccgt ccaggctgcc gcggatcttg atgtccatga ggacgaggtc
    14221 gggaccgagc tcgtccatgc gcgccaggag ctcgtcgccc gaggggaaga gccccgagac
    14281 ccggtagccg gagcgctcga ggaagttctt gatgtcgagg gcgacgatgt actcgtcctc
    14341 tacgacggcg atcgcgggag actccggcaa tgaggccacc tactccctcg gggcgaagcc
    14401 cagcttggct atctcttccc tgagcctctc gcggtcgatg ggcttcacga ggtagctcgt
    14461 cgcgccgccc tggtagtagg cctcgatgac gttgcgcgga tcctccagga cgctcgtcat
    14521 cacgatcttc gcctcctccg cgggcttcac cccgagctcg cgctcgagct cgcggatgcg
    14581 cttgagcgcc tcgtggccgt ccatcttggg catcatgatg tccatgaaga cgaggtcgta
    14641 gggcttgtac tcgtcccagg ccacgcggaa ggcctgcacc gcctcctcgc cgtccacgac
    14701 gacgtccacg tcgccgtagg gggccaggag cttctgcatc atcctccgcg agccgaaatc
    14761 gtcctcaacg accagtattt tcacggcggg cccctccttc gagcctcatg agtccacgcg
    14821 cggaacggcg gccgcaccca ggcggcgcgc cctctctatc acgccccgga ggccctcgcc
    14881 gtctcccttg cgggagagga ggagggcgga gaaggcgagg cgctcgcagg attccgctcc
    14941 gcgggcgacg aaaagctccc tgtattcctt ggagacgcgc tcggcggcgg cgaagtcgag
    15001 gctctccagg ctcgcctcta ggcgcgcggc caggttcccg agctcggcgc cctcggcgag
    15061 ctcgccctgg acggcggcca cgggcgttcc gccggcggcc acggacgcgc cgcgggccgc
    15121 tgaaggcgcg gcgcctttcg ccagaggcgc gccgcgggcg gccggcgcgg cgaccgcggg
    15181 ggcggcagga gcgacaggaa cggcggctga cgcgccccgg gccgcgctcc tggccggtcc
    15241 tcgagttgcg gctttcgacg atcccagggg cgcttcggcg tggccgtcga gccaggcgtg
    15301 gcagagatcg cggtccccga ctccgagctc caggagggcc gagacaaggt ggcgggcgcg
    15361 gacaggaagg ggcaggacgg gaccctcgcg gcgcttcgcg ggaccggagc cgccgaagcg
    15421 cctcgcgacg accagcggca ggggcctggc accgtccaag gcgcccgccc cgcccttcac
    15481 ggcggccccg gccagggcga gttcgtccgc caccaggcag tcgagggccg gcccttccgc
    15541 gccggcttgt ccgggcaagg cagccgcgcc cagggacgag aggagctcct cgagatcgcg
    15601 cctcgcctct ccttcgaagc cgaggaggcc gatccgcagg ccggagaggg gccgccctcc
    15661 cgcgccgtcc gcggaggcct ttccgtcgcc gcccggctcc gcgctccccg cggggaggcc
    15721 gaaggggagg atgagctcga agcggctgcc cttcccggga gcgctcctca cggcgacgtc
    15781 gcccccgagg agctcgacgc tcctcgcgac tatggccagt cccagccccg tgccgcccgc
    15841 cgagcgggtc ctggatccgt cgagctgggt gaagcgcccg aaggcctcgc cgatccgctc
    15901 cgcgggcatg cctatccccg aatcctccag ggcaaagccc aggaggaggc cgcccggagg
    15961 gggcttgggg aaggccgccc cggacgaggc tcccatgcgg aactcgtccg ggcggcccgc
    16021 cgccgctccc tccggaaggc ggccgacctc gaggcgcacc ccgccccgct cggtgaactt
    16081 gagggcgttg tccgcgaggt tgtggagggc ctgcttgagc cgccccgggt cgcccacgag
    16141 gaaggccggc agggagggat ccaggcggaa ggtcgcctcg aggcccttcg aatgcgcgcc
    16201 cacggccagg acgtcgacga tgccgtccag gaggccggcc agggagaagg cctcgagctc
    16261 ggcggagagg cggcccgact ccaggctcgc gaagtcgagg atggagtcga tgagggctga
    16321 gagcgtcttc gcgccggcga gggccgagga gaggtagtcc cgctgctcct cgtccgcggc
    16381 cttgtccagg gcgagctccg tgaagccgat gacgctgttc aggggcgtct tgagctcgtg
    16441 gctcacgttg gcgaggaact cccccttgag cctgctcgtc tcctccgcga attccttggc
    16501 cttgcggatc gagagctcgt actggaattt gtagagggcg agctcgatcg cgatgcggag
    16561 ctcccgctcg tggaagggct tcacgatgta gccgtagggg ctcgcgagct tggcccgctc
    16621 cagggtcttc tcgtcggtga aggcggtgac gaagatgatg gggaggtcga cgagctcccg
    16681 cgcggccctg gccgtctcta tgccgtccgc cgcccccttc agcttgatgt ccatgaggac
    16741 gaggtcgggc ggctccggcc ccgtcatggc ctccagcgcc gccggccccg agtcgacgac
    16801 ggccctgacc ccgtagccaa gggcctccag gcgcatccgc atgtcgaggg cgacgatgtt
    16861 ctcgtcctcg acgatgagga tgcccccctt cctcacctgg cctctccgct cgccttggcg
    16921 gcgtcggcga aggccgcgtc cacgtccagg atgacgatga agcggccgtc cttctcggct
    16981 atgccggaga tgaggccctc ctggccgcgc ccgccgaagc ggggggcccg gtcgatgcgg
    17041 gagggatcga tgtccacgac ctcgcgcacc gcgtccgcga gcacgccgat ggtcaggtcg
    17101 tccccgccgt agtcgatgtg gagcacgacg atgcccttgc cctcgggctc ggcctcggaa
    17161 gcggcggccg cggccaacgc cttgccctcg gcctcggcga gctcctgggc caggccgccg
    17221 aagcggctcc gcaggtcggc cacggggatg accgtgccgc ggtagttgat gacgccgcgg
    17281 aggtgggcgg gggccttggg caccctcgtt atcggcatcg gctccaggac gacctcgacg
    17341 cgctcgacct cgagggcgta ggactccccg ccgagctcga aggaaaggta gcggccgctc
    17401 ttctcgccgt tcactcgcgt cctcccgtcc tcactccgcg accttcttga cgatgcccag
    17461 gagcttgtcg gcgttgaagg gcttcacgat ccagccggtg gcccccgcct ccttgccctc
    17521 gttcatcttc gagccctggg actccgtcgt gaggacgagg atcggggtga acttgaactt
    17581 gggcagctcc ctggccttgc ggatgaagcc gatcccgtcc aggttgggca tgttgacgtc
    17641 ggtgatgatg aggtccaccg cgtcgagggt cccgagcacg ttcaaggcgt cgaggccgtc
    17701 ctgcgcctcc accgtgtcgt agccttcctg cttcaatatg aacgagatgc tctgcctgat
    17761 cgcggccgag tcgtctacga cgaggatctt cttggccatc ttgcttctcc ttgtatccgc
    17821 tggccgcgtc cctggcggca ggcttggctg tcgtatacct agcgcgcgcg gcgctggact
    17881 tcctgggcga tgcccccgag gggcaggatc acgtccactc cgccgagctt gatggcctcg
    17941 ttgggcatgc cgaagacgac gcaggaggcc tcgtcctggg cgatcgtctg cgcgcccgcg
    18001 tcgtggagct ccttcatgcc cctggcgccg tcgtcgccca tgccggtcat gatgacgcct
    18061 atcgcgttct tcgcggcgta gcgcgccgcc gagcggaaca ggacgtcgac gctgggccgg
    18121 tggcggcaga cgagcgggcc ttccttcacc tcgacataat agcgcgcgcc ggacctcttg
    18181 aggagggtgt ggcggttgcc cggggcgatg agggcgcggc cgcggatcac ggtgtcgttg
    18241 tcctcggcct ccttgacgtt gatgcggcac agcccgtcca ggcgcatggc gaaggcggcg
    18301 gtgaagtgct cgggcatgtg ctggacgatg acgatgcccg gcgcgtcctc ggggaagagc
    18361 tccaggaatt cccgcagggc ctcggtgccg ccggtggagg cgccgacgac gacgaccttc
    18421 tccgtggtct ccacgaagac gcgccccgcc tcgggcttgg gcagcatgac gtcggcggtg
    18481 agcttggggg agacctcgcg gtccaggctg cgctgccggc cggcctcggg gcgggcgggg
    18541 gcgacgaggc tcgagagctt gttccgcgag aggtaggccg ccttcaccgc gtcgcagatc
    18601 ctcaccttcg attcctcgat gaactgcttc gtgccgagct tgggcttctg gatgatctcc
    18661 acggcgccgt actccagggc cttgatcgcg ttctccgagc cctcctcggt cttggaggag
    18721 cagacgacga cgggcagcgg cgccgtctgc atgatcgacc tgaggaaggt gagcccgtcc
    18781 atccgcggca tctcgacgtc ggtgatgatc acgtcgggcc gcatggtctc caggtgcttc
    18841 tgggcgaaga tggggtcggc ggaggcgttg acgacctcga tctgggggtc cgactcgagg
    18901 acctccctca gggtctgcct caccacggcc gagtcgtcga cgagcatcac cttgatagtc
    18961 tgcttcattg ggcctccgct gttcccgccc taggcgcgcg agtagatggt cggcgcgacg
    19021 ctccggaggg gcaggtccat gcccgtcagc gtctccgaat gcccgaggat gagccatccg
    19081 ccggggcgca cgtacctgca gatccgcccc aggatgagct cctggacggc ccgctcgaag
    19141 tagatgatga cgttgcggca gaagaccacg tcgaagagcc cgtctatcgg gtagcgctcg
    19201 tccatgaagt tgatgcgcgc gaagctcagc ttggccctga gctcgggctt gatcctgacg
    19261 aggcccgagg ccgggtcctt gctccggagg aggtacttct tcttgaagct catggggatg
    19321 ggggccgcct tctcctcttc gtagacggcc gccttggcct tgtcgaggac ctgggtggac
    19381 aggtcgctcg cgaggatcct gtaggagaag cgggggttcg cctcgcggaa ctcctccagg
    19441 accatcgcca gggtgtaggg ctcctcgccc gtcgagcagc cggccgacca gaaggacatg
    19501 gtcccgccgc gggcggcggc cgcgggcagg atggactcca ccaggtactc gaagtggtcg
    19561 gcctcccgga agaagtcggt cttgttggtc gtgacggcgt cgatcatgtt gatgagctcg
    19621 gactcgcggc cctggggcga gaagacgaag tccacgtact cggtgaagcc ggacatcccg
    19681 aagtggcgga tgcgcttctg cagcctggac tccagcatca cgcgcttggt gtccggcatg
    19741 cgtatgccga gctcgctctc gatgaagccc gcgaggcgcc ggaaatcccc gtcgccgagc
    19801 gacgagggag gcgccactcc cgggacggcg cggtagctcc ccggctcgct catcgctccg
    19861 atcccttcgc ggcctttcgc gcgctctccc gctggacggc gtaggacagc cggttcacgt
    19921 ccaggatcag ggccaccgtg ccgtcgccca ggatggtggc gccggagacg ccctccacgt
    19981 ccttgaacat ccgccccagg ggcttgatga cggtctggta gtcgccgacc acctggtcga
    20041 cgacgaagcc gatcttcgcg tcctgggccg cggcgatgac gatctgctcg atctccggct
    20101 cctcgcccgg ggtctcgaag gcctcgcgga gcgggaggaa gggcacgagc tcgtcgcggt
    20161 aggagaggat cctccggtcg ccgtaggccg cgagctcctc gcgccgtatc tcgatgcagc
    20221 cgtctaccga ggagagcggg atgacgtagt gctcgccctc gatgcggacg aggaggccct
    20281 cgatgatcgc gagggtgagg ggtatcttga gggtgacggt cgtccccttc cccttctccg
    20341 tctggaggct caccgagccg cccagggagt cgatctcccg cttcacgacg tccatgccga
    20401 cgccgcggcc cgagaccgcg ctcacgacct tcgcggtgga gaagccgggc gcgaagatga
    20461 ggaggtagag gtcctcgtcg gcgatctcct ggccggggga caggatgccc ctttccacgg
    20521 ccttcccgta gatcgcgtcc ttgtcgaggc ccttgccgtc gtcggtcacc tggatgagga
    20581 cgtaggcgcc cgagtgcctg gcggagagga ggatgctgcc cttctcgggc ttgcccgcgg
    20641 ccctgcgcac gtccggccgc tcgatgccgt ggtccaggga gttgcggatg atgtggacga
    20701 gggggtcgcc gagcttctcg atgacagtct tgtcgagctc ggtctcagcg ccctcggtga
    20761 ggagctcggc ctccttgccg agctccaggg agaggtcgcg gacgacgcgc cggaacttgt
    20821 tgaaggtgct cccgatgggg agcatccgga tcgacatcgt gttgtcgcgg agctgggcga
    20881 tgagccgctc gaactgctcc gagatggagg agaggggccc gtcctggagg tcgagggagg
    20941 tgcgggagag ccgggcctgg agggtgacga gctcgccgac caggtccacg agggcgtcca
    21001 gcttgtccga cgcgaccctg accgagccca tgccggcctc gccgcgctcc tgcaccttct
    21061 tgaggtgctc ctgctccacg agggccgact cgatctcggg cttcgtggcg atgtgcttct
    21121 cgacgaggac ctcgcccagg cgcttctgcg agcccagggc ctcgcggagg gagtccgcgc
    21181 tgacgatccc gcggtccacc aggatctcgc cgagccgctt ggagcgcccc tcctcgtcca
    21241 ggggggcctc ctccaccctc tcgatcctga gctcggagct gtcctccacg aagatgaaga
    21301 cgtcgcgcag gtcgttctcg ctcttcgccg tggtgaggag gacatcccag tagcagacgc
    21361 atttctcggg atcgagggcg tcgagggaga ctatcgcctc ggtgtgcggg tagaccgcgg
    21421 cgctgccgag ggaggaaagc tcctccacga ggttgagcgc cttcgtgccg tccatgaaga
    21481 tgtcgggctt gggccgccag aagacgcggt aggtgacaag gcccgagggg gcggccggcg
    21541 cctccgccgc cccggcggca ggctcggccg cgggcgcctg ggcttgggcg ggagcttggg
    21601 ccgcggcggc ctggggctgg gaggcggccc ggggagcggc ggaggccgcg ggggcggacg
    21661 gcgcagagga ctggggagcg gctggggccg cctgcgccgc ggcctcggcc gcggccgcct
    21721 ggggcttacg gcccgcgtcc gtcgccgcgt actcgcggta gcgcagcagg atcccctcgc
    21781 cgtcgccgga gacctcggcg ggaggcggct cgtcgaattc gaggaggcgc cgcaggtggt
    21841 cgcgggcctt gagggtgagg ccgatgaagt ccttcgtgat ggggagggtc ccgttcctgc
    21901 actggtccag gactgactcc acctcgtgcg tgaacttcga gatggtctcg aagccgaaca
    21961 tcgcggagga gcccttcacg gtgtggatgg cccggaaggc cgcgttgatg agctcgccgt
    22021 tgagcggctg cgactcgagc tcgagcaggg tgtcctcgag ctgggagagg agctcgtagg
    22081 actcctcccg gaaggcggcc ttgaatttgt cgatcatcgg agctccctca actcagaaat
    22141 ccaagagccc ggcctcgaag tcctcggccc gctcgacgaa accccgcagg aagccgcagg
    22201 ccacgagacg cttggcgacg cggggcttca cggaccccgc gaaatggaat tccctgcccg
    22261 aggccttggc gctcctgcgc gccgcgtaga ggacctggag gcaggcgagg tcgagctcct
    22321 cgagctcgga gaggctcacg aaggcgggct cctggccggc caagagggcc tggagctcgc
    22381 tcttgagccc ggcggcgcgg tcgacggtgt aggcgccgct cagggcgaga gtcttgggcg
    22441 ctgctttctt cggtgtcggc atagggctcc cttgagacta ctatagactg tagcccgtaa
    22501 atttcaagcc ttaagggagc tttggccggg gccggccagt gccgaaaggc ccgccaagga
    22561 gggaaaaccc gctagatgaa gtcgggtatg tccagggggg cgggaatctg gatgatgctc
    22621 tccaggaagc cggccttgcg cttcttcacg tggatctgga tcaccgcggc gcggagctcc
    22681 ttgtcgacga tgaagcggcg cagggcgaag gggtcgttct cggagaggcc gctctcgtcg
    22741 gctatggagc cggggtagat cttcgccacg ctgtaggcct cgggctcgag gaaggagccc
    22801 ggcaggggcc gcacgctcat cccgaagagg gccgggaaga ggaggtcgcg tcggtcgagg
    22861 gaggcggcgg cctccagggg cgagaagggc ctcttcctca gggcgcgcag ggccgaggag
    22921 gcctcccctt tcgggtcggc gccccccgtc ttgaccaggg cgagctcccc cggcgagcgg
    22981 gagaggaggt aggcctgggc ctcgacgatc tttccgaagg aagcgccgtc caccgacagg
    23041 aggcgggagc ccgcctcgag gccgggcggc gccgagggat ggcgatagct gatctcgagg
    23101 gccttgccgc cctcgctatt gggcgaggcc gagcccaggt cggcgaggga gaggccgagc
    23161 caggcccgct ccacctcgct gcccctgaag agggcgggga ggaccctggc ggcccaggcc
    23221 gcggggatgg cgaagttgag gccctggaac tggggcatgc ccgcgaagac cacgccggcc
    23281 agggcgcccg actcgtcgac gagggggccg cccgagttgc cggggttgag ggccgcgtcc
    23341 acctgcatgg cctcgcccgt ctggaggagg cgccggccgg aggccgacac gatgcccgcc
    23401 gtcacggtgt tctcgaggcc gaggggcgag ccgatcgcgt agatccggtc gcccggcccc
    23461 ggcgcggcgc cggcgccgac cgggaagacg tactccggcc tcgcctcgac cttgatgagg
    23521 gccaggtcga ggaggcggtc ccagcccacg accttggccg gtatccgcgc ctcgggggcg
    23581 tcggagggcc tcacgctcag gcgggagtag ccctcgaact tgggatcgac ctcgctctct
    23641 atgacgtggt agttcgtgag gacgtagccg ccgccgtcga tgtagaagcc cgagccgagc
    23701 atgcggtcgg gactcccgag gccctgctcg atcttgatgc cgcggtccac gcggatcgtg
    23761 acgacgcctt tgcgcatgtc cgccatcgag tcgcggctcg aaaggagggc aagggccttc
    23821 tccggcaggc cgaggcccct cgcggcgagt tcgcggccca ggcgggagag gacggccctg
    23881 ttcctggcct cgagggcacg gccggcccag aggaggagct cctccttcgg cgccgcgtcg
    23941 gcggcaagcg gcgcgccggt cgcggctcca tcgccggccg agccggcggc gcctgctttg
    24001 cccgccgcgc cggcggcgga catcgctgag ccggcggcct ccgccgctcc aaggccgcgg
    24061 gcgtccaggg actcgaggta gaggaggagg gccggcgtgg agaggccctt gccgtagaag
    24121 gcttcggcct cggcgacgag gagctcggct tcccgcgcgg cgggagcgcc ggccagcgcg
    24181 gccgcctccg gcctgagaag ggaagccgag gcggggaagg cgcccaaggc cttgaggctc
    24241 cgcaaggcgg agagggcgcc gcggggatcc ttggcggcga gggcagcctc gtggcgggcg
    24301 gcgaagcccg agccggcctc ggagacgatg gccttgagct caaggacgag ggactcgcgg
    24361 ccgcctgggg caacgccgtc ggcgagggct tcgggacgcg cgaggaagta gagggcggcc
    24421 tcggccgcgg ccgcaggatc gtctttgagg aaggcgcgga tctcctcgag cttgagcctc
    24481 tcgtagctca aggcggggct ttcgcccttg ggggccgggc cggggctggc gcaggagccg
    24541 aggaggaccg cgaggaggat ggcggccggg gcgcggaggg cgaggcggag cctcgcggcg
    24601 gaaggcctca aggcaggagc tccgcgaagg cttcggggcc cgcgtagacc aggcgatagc
    24661 gctgcttcat ataccggtaa accccttcct cgggcggcag gttacttccg aggtcgaagg
    24721 gcttcgaacc tatccacctg agccggggat tcttgtcggg aacgagggcg ggcctggcgc
    24781 cgttccgcag gaaggacctg atcgagcccg aggcgtcgag ctcgagcctc tcgtcgaact
    24841 cgccgtccag gcggctcgag aactcgcgga ccaggccgcc accggccagg gcgtattgcg
    24901 cggcgtcggc caggccgtcc gagtccaggt cccattcctt gcggtagggg aagcggagct
    24961 cctcgcggta ctcgaagagg ccgtccccgt ccgcgtcgac cctggtccag gagacggcgc
    25021 cctcttcgta ggcgccttcc gcgtccagga aggggcgggc gccgggcggc aggggggcgt
    25081 cgcggcccct ctcggtctcg aagcgcccgt cgccgtcggc gtcgaggctc tccacgaggg
    25141 ggaggccccg ctcgaaggga aggctggagt acagccgccc gtcgcgcagg cggtcgagcc
    25201 tgaggggcgt gccgccgtcc aggctgacgc ggatctcctc cccggggccc aggactctga
    25261 gaagataggc ggcggaggcc gccgccctct ccgtgggctc cggcggatcg agaggcaggg
    25321 gcagatagag gccgtcgtcg agggggctcg ggaagggcag gaaggaaagg agaggaaacg
    25381 cgaaagcctc cggcgcgaag gagtattccc ggatccgcga ggggtctttc gggggaagtc
    25441 cgcgcccggg gacgccggga tcgggcggca gggcctcgaa gcggaggctc tcgacggcgg
    25501 gataggcgcc gtaggcgagg cgcaaggaat agtccttgcc cgcgagaaga gcctcggcgg
    25561 gaagcccgtc gcggaaggag acgagccggt cgggccggcc gtcctggtcc gcgtcgagcc
    25621 tgaaggaggc gaggcgcccg tcgcggtaga gggccgtctc ctcgcgttgt ccgtcgccgt
    25681 cccggtcggc ggcgaagggg cccgaatagc ggcgcagggc cgctgagagg gcggccctcc
    25741 ccttctccgt gccgacgagg gcctcgaggc gctccaggaa gccgggactc aggggctcgc
    25801 tccccgcgaa ggcctcagcg gtcgcggcct cctcggagag aaggccgtac tcgagggcga
    25861 gggccgtgga gcgcaggccc ctgccgccgg ccgcgcgata ggcggcgacg gcgtcgcgcc
    25921 tggcctccgc gccggccatc caggggacgg agaggaggag gagttcggga tcgagggagg
    25981 agaggccggg cttgccgccc ctgtccccga gcctgcccag gaaggccgcg gccaggtccg
    26041 cctcggcctt cgatgcgccg gaaggggccg cgcccgcggg gccgcccgag ccggcagctc
    26101 cggcctcgcc gcgctcgaag aacagcctcg cgaagcgggg atcgtcggga tggcgccgcg
    26161 cggccgcctg aagggcggcg acggcctcgg gccggcggcc caggccgtag agcgcgaggg
    26221 cgcggaggcg cagggcgtcg gaggcacgcg aggggaagga ggccgaggcg gctgcgggcg
    26281 cgcgctcgag ctcgtcgagg gccgcgaggg ccttctcgaa gcgcctcgtc ctcacgagga
    26341 gctcggcgcg gaggaggcgg gcggcgcccg cctcgaacat ggaaaagcgg ccggagagaa
    26401 gggcggcctc cagggccgcc agggaggcgg ccgcgtcgtc ctgggacttg agggccgcca
    26461 gggcccacag atagaggagg tccgagtcgg cgccgtcgta ggagaggccc tcggcggcga
    26521 gctcctcggc ctcggcccag ccgcccttcg ccgctaggcc ggccgcggcc tccgcgtaag
    26581 gcgaggccgc gggggttgcg gccaggcccg ggacggccgt cccgaggagg agcgcggccg
    26641 cgaggccggc gaggaggcga ggaagggtcc cggggccgcc cgccgccctc acggctaggc
    26701 ctcctgcgcc ttgcggggcg ggaagccgaa gaggttcctg atgtcctcgt ccaggagggt
    26761 ctccttgacg atgagctcct cggcgaggcg ctcgagctgg tccttgtgct cggtgaggat
    26821 ggagacagcc cggcccgcgg ccttctccag gaagtcgcgg acggagccgt cgatcctcat
    26881 cgccgtgtcc tcggagtagt ccttgtggcg ggctatctcc ttcccgatga agatgggctc
    26941 gtcctcctgg ccgtaggaga cggggcccat ggcctccgcc atgccccatt cgcagaccat
    27001 cttcctggcg aagtccgtcg cctggcgcag gtcttgggcc gtgcccgtcg tcgtctcgcc
    27061 gtagacgatc ttctcggcgg cgtagccgcc gaaggcgaag accatgtagt cctcgagcca
    27121 gctcttggtg tgcgagtagg cgtccttctc gggcagggcc agggcgaggc ccagggcccg
    27181 gcccctgggc accacggtga ccttgtgcag ggggtcggag tgctcgaggt agtagagggt
    27241 gagggcgtgg ccggcctcgt ggtaggccgt ggcgcgcttc tcggcgtcgg tgatgacgag
    27301 gctcttgcgg gcggcgccca tgaggatctt gtcgcgggcc tcctcgaagt cctccatgag
    27361 gaccgtcgtg cggtccttcc tgatggcgaa gagcgcggcc tcgttgacga ggttcgctag
    27421 gtcggcgccg ctcgtgccgg gcgtggagcg cgcgacgagg cgcgagtcga cggaggcgtc
    27481 gacggggatc ttcttcatgt ggatgcccag gatggcctcg cgctcggcga cgtcgggcat
    27541 cgcgacgacg acctgccggt cgaagcggcc ggggcggagg agggcggggt ccaggacgtc
    27601 gggcctgttc gtcgccgcca ggatgatgac gccgtccttc gtgtcgaagc cgtccatctc
    27661 gacgagcatc tggttcaggg tctgctcgcg ctcgtcgtgg ccgccgccgt agcccgcgcc
    27721 gcgggtgcgg ccgacggcgt cgagctcgtc gatgaagatg atgcagggcg cgctgcggcg
    27781 gccctggtcg aagaggtcgc ggacgcggct cgcgccgacg ccgacgaaca tctccacgaa
    27841 gtcggagccc gacatgtgga agaagggcac gttggcctcg ccggccacgg ccttggcgag
    27901 gagggtcttg ccggtgcccg gcatgccgac gaggaggacg cccttgggga tgcgcgcgcc
    27961 catcttcacg aacttctggg ggttcttgag gtagtcgacc acctcctcga gctcgtactt
    28021 cgcctctttc tgtccggcga cgtcgtcgaa ggtgatcttc ttggcgggct cgacgtagcg
    28081 cttggcccgg ctcttgccga agctgaaggc cttgttgttg ccctggacct ggcggagcat
    28141 catccagagg atgaagaagc cgaagagcca gggaaggagc tcggcgagga gctgccaggg
    28201 cgagacgccc ttgaagccgc cggagacctt cacgtccttc tcgcgcaggg cctttaggag
    28261 ctcgggatcc tcgtagggga tggtcgtgcg gcgtagggcc tcggcccccg ccttgccgcg
    28321 gagggagagc tggatctccg agctgtcgag gatcttcacc gcctccacct cgccctggtc
    28381 caggtgggcg aggaaggccg agtagggtat ctccggggcg ctggagcggt cgttcgcgaa
    28441 gaagagcgcg gagaagagca cgcccaggga gaggaaaacc gcgagggcgg cgcggctgcc
    28501 gcggttgggg ccttcgggcc gcgcgccctc gctcttgcgc ggaggctggg ggttctgatc
    28561 gtcgaagtcc atcgttaagc caaggctcct ttcaccgcga tagaaagctt gcgcccgttt
    28621 ccaaggccgc cgccgcaatg acggaacctg tccctgccgc cccaggcgga gcccaggacg
    28681 gcgacgatgc cgtcccggtc ctcgacgacg gtcagggagc cgcgggcctc cgcggggagg
    28741 ccccattcgg acaggagctc gtcgaggggc ttcctgccgc ggcgaaggga gagagcgtcg
    28801 ccgggccggc ggctgcgcgc cacgaggggg aaggtgaagg ccccctcgtc gaggcccgcc
    28861 gcatccggtc cttccgacca acgcgcggcg accgtgacgg atcccgcgtg gatctccgcg
    28921 ccgccgggcc cgggccgttc gatgactaca aaatacccgc gccgtttggg aaaatcaagt
    28981 gagcgggaga ggatgaggcg gccttcctcc agcctgagct cgattccggc gccccggtag
    29041 cgtccgcccg acgaggccgc gaggagggcg gcccgcgcga ggcgcgcggg gaggcggcgc
    29101 ccctcgggct ggcgctctcc cctcctccct ggccgcgcgg ggccgaggag ggacatcgcc
    29161 tgaaggaggg cgcgccgcct cacggcctcg ggcgcgccga agaaggaggc cgcgtcggcc
    29221 tcgagccggc cggcggccga cgagccgggc gcgcgcggag tcgcggctcc gccaggacct
    29281 gcgcccgacg aggacgcggc cggcgcgggg gcgaagccca cgcggacggc ccaggcggcc
    29341 agggcctcct cgtcgagggc ggctagctcc gcgccccgca agaggccccg tccccagccg
    29401 gggaagcgct cgtccaggac gggcacgagg cccctcctca cgcggttgcg gaggtatgcg
    29461 tccccgtcgt tggtcgagtc ctgggagtgg cccaggcctc gggccgcgag gtaggcgagg
    29521 atctccgact tgggcgtcga aaggaggggc ctgaggatgg gccccgccgc ctccggcatg
    29581 ccgcggagtc cgcccggccc agagcccgcg aggaagcgca tgaggatggt ctccagttgg
    29641 tcgtcgcggt tgtgggcgag atagaggcga gacaggccct cgcgggcgag gatggcgcgg
    29701 aaggccgcgt agcggaagcg gcgcgcagcc gcctccacgc cctcgccatc ggcccgcgcg
    29761 cgctcgagga cggcgcccgg cctgaggcgc gcgatcgtaa ggggaacgcc gagcccgcgg
    29821 cagagcccgc ggacgaagtc ccgctccgcg tcgagctcgg ccctgggcct gaggccgtgg
    29881 tcgacatgga cggcgcgcag ggctcccgcc ccgagggcgg cgagggcggc gaggagggcg
    29941 gtcgagtcgg ggccgcccga gagggcgacc agggccggct cggccttgga gaggccgcgg
    30001 gaggagagga agccctccac gcgggcttcg aaggccttag gatcgaggct ggcctgcacg
    30061 acgcctcctc gcggaaaaag aaaaagagag aggatcgctc ccctctcttc cccggcgcgc
    30121 tccgggagga cagcctcccg gccgctggcc ttacttcttc tcttccttct tcgcggcggg
    30181 cgcggcgccg gcggcgggag cggctccggc ggcaggcgcg gctccggcgg cgggagcggc
    30241 tccggcggcg ggagcggcgg cctcggcggc gggagcggcc tcgacctcga ccttggcgaa
    30301 cttgacgagg gctacgacga ggtcgccctt cgtgaggatc ttgacgccct tgggcgccgg
    30361 gatgtcgcgg acgtggatcg agtggttggc gtggaggccg gagacgtcga cgtcgatctt
    30421 ctcggggagg tccctcggga agcactcgac ctcgatctcg tgggccgggt tctcgaggat
    30481 gccgccgtcg gtgacgccga cgggcgtgcc gacgaggtgc acggggacct tcgcgtggag
    30541 gctgcggtcc tggaggacct cgaggaagtc gacgtggatg atctccgtcg tgagggcgtc
    30601 gcgctggcgc tccttcacga aggcttcctt cttgtccttc ccgatattga tggtgatgat
    30661 cgtggactcg gagatgccgg ccgtggcctt cttgaactcg agggcgtcga gggtgacctg
    30721 ggtggccgcg aggccgccgc cgtagatcac ggcggggatc ttgccgttga cgcggaggcc
    30781 gcggagctcg cccttcgtcg tcccctcgcg gagggcggcg ttcagaacgg gatgattcat
    30841 gatgaagact ccttactatc gaaactgtcg aagcgtaact atatcgatgc tgggacggca
    30901 ggattcgaac ctgcggatac cggaaccaaa atccggaggc ttaccgcttg ccgacgtccc
    30961 aatgagcgac gcgagaatcc gcccccctag tcagtcgaaa tccgcgggcg tcgcctccgg
    31021 gtggggtccg ctctcgaagg tcttgagcac gcgctcctgg agacggttgc ggaactccgg
    31081 ggtgatggga tgggcgatgt ccttgtactc cccgctgcgc gtcttccggc tcggcatcgc
    31141 gatgaagatc ccggtcttcc cttcgatgat cttcacgttg tggacgacga agcagtcgtc
    31201 gaaggtcacc gtcacgtagg ccttcagctt gctctcggtc gtgacccttc ggatgcggat
    31261 gtccgttatc tccatgctgc gactctcctt gcggggcgcc tgcggatgcg atcctaattc
    31321 tactccaggg aaggaacgca cgcaagcggg agggtcagcg cgaccaggga gcctccgggg
    31381 agaccggcct tgagcgcctc ggcggcgaag ccggcggact cgggatccgg gaagactccg
    31441 aagaccgtcg atcccgagcc gctcatgcgg acgaaggggg agccggagcg cctcaaggaa
    31501 gcggagaccg aggcgatcgc gggatgggcg ccggcaacgt agggctcgaa ggagttggcg
    31561 aagggccagg acgagggttc gcggcggtag gcggcgagga gctcggaggc ctctgggtcg
    31621 gcctcgcctg agtcgtcggg gcgctcgcgg tccaggaggg cgaaggcggc ctgggtgctc
    31681 accgggaagc cggggaaggc gacgaccagg cagaagtcct cgcggcccgg aaaggggctc
    31741 acgacctccc cccggccttc cacgaccgcg gcggcgccgc gcaggaagaa ggggacgtcg
    31801 ctcccgatcc tggctccgag cgaaaggagg gtctcctcgc caaggcggcc gtcggcgtcg
    31861 ccgaagaggg cgtccagggc tttgagtacg gcggccccgt cgctcgagcc tccgcccatg
    31921 cctgctccgg ccggtatgcc cttccctacc cgaatcctga ctcctcggct ggatcccgtg
    31981 gcctcgcggt agaggcggac cgccttgtgc acggtcgtcg cctccggcgg acagtcgaag
    32041 gttccgtcga tttcgattct atcgggcgat gtcaaagaac cgacgacaat ctcgtcggcg
    32101 agggagatgg cttggaatac gctccgcagg ccgtggaaac cgtccgccct gcgcccgtag
    32161 acgcggaggt ggagattgac cttggcggga gcccggacca ggacagtctt ctccatcgcg
    32221 cggctattat accgatacga cggggattgt caaaccctcg aataaaggag cgatcgtagt
    32281 tgacagaacg gcgtcggctc ttttagattt tcagctggcg ccggccctcc ccccctccac
    32341 gcgaagtcgg ggaccaccgg cccgatgcgt cggcggcctt gagtcggaaa gaggcgccaa
    32401 ttcacaggga ggaatacgat atggtcccta cctacggaga aaccgcgatg gatccggccc
    32461 tggagccggc gctcgcgccc ttccagggta tcgcccccgc ctatccggtc gcgcgcttcg
    32521 acgacggctt cgacgagtac gaggacgacg acttcgaaga ggacgaggac ttcgacgacc
    32581 tcgacgacga ggacctcgac gacgaggatg aggatttcga cgacgacgac gacttcgacg
    32641 acgattccgg cgacggattc gaggacgagg acgaaggcga cgaagaagag gagatggatt
    32701 acgaggacga cgtcgactac gacgacttcg acgagtgatc tagcctgagg aaaggctccc
    32761 gacgcgaagg ggccgcctcc ggacgccgcc tgccatggcc gcgccggagg cggcccctca
    32821 aatttctccc gggcgttccg cttcaggcgg cagcgccagg cggcctaagc agggcagccc
    32881 cgcggcggcg gaggccgcgc gcctggccgc cccgggcctg agctacgcgg gccccggcgc
    32941 cgccggagcc tcgacgctcg tggaggcggc cttgaaccag agcttctcga gctcgtagaa
    33001 cgtgcgggcg cgctcggtca tgagatggac gaccacgtcg cccaggtcca ggaggaccca
    33061 ttcctcgtcg tcctggatct tgggcgtgtt gagcctctgc accttgttct gcgaggccca
    33121 ttcgtccgcg tagcgggcga ggcccctcaa gtgggtcgag ctcatcgccg tcgcgatgac
    33181 gaaaaagtcg gtccagcccg actccgcggc caagtccagg accaggacct ccccgccctt
    33241 gtgttcggcg agcgtccggg cgatcgagaa ggccgattcg cggctatttg gcacagtacc
    33301 tcccattgaa gtctttccct agaatgacgg tgaaatcggc caccgtctcg ccgggcatgc
    33361 ccgcttgcgc ggagacgctg gagcagcgga tcacgtccgc gacgttcttg gccgcctcgg
    33421 ggttcgagaa gcggtcgatg acgagggtcg atccgtagtc ctcccggtcg gcgttgccca
    33481 ccgagaccac gtcgtagccg aagctctgga agatctccgc agtccgcttc gcgaggccct
    33541 tgcccggggt gccgttgagg atctccacgg tgaagatctt gtcggccacg gcgtcggcgc
    33601 ccgagctcga gagggcgttc agcgtctgct tcacgatgtc cttgacgagc tcgccgtcgt
    33661 agtgcgggaa gaggagccgc ttgccgtcca cctgcctgaa gacgcccgtg acccgctgga
    33721 gcacgaggcg gtcggcgtcg agcttcccga gctccgcggc gaagcggcgc gagaggtcgg
    33781 ccgagaggtt cgtgcggagg aggcggttga aggcctggaa ggcctcgggc cgctcgagcc
    33841 aggaggcctt ctctccgatc cggcggagga gggactggag gagcttctgg cggcgcgcgg
    33901 ccgcctcgcc ctcgccctcc tccgcgtcgc ggtaggacgc gtactggacg agcttgtcgc
    33961 cgtccaggac cagggctccc gccgggagga ggacgcgggg cggtcccgcg ctcgcgaggg
    34021 gctgggggat gaagagctcg acgccttcca ggaggtcgac gagggaggag aggctcttct
    34081 cgtcgaggac gatccagtac gggacctcgg cgtcgaggag gttcgagacc tcgtcgacga
    34141 aggtcttggg gcggcgcggg tcgtagaccg cgtcgatgcg gtccaccctg ttgagggact
    34201 tgaggatgag gcccgtctcg ccggggacgt cgaggagggc gcccttcccc gtcgaggggt
    34261 acatgaggca gagctcggtc gccacgggct tcccctcgag ctcgaggacg aagagcacgt
    34321 tgagtatccg gtcttccttg accgcctcct ccgccgcgtc ggagcgcagg gagaggccca
    34381 ggaagacgag ggtccccgcc aggaaggcga ggatcacgaa gaggaggacg gagctcttgt
    34441 ccaaggtcac tggcttcatg tctgctgcac cgaatcgcga agggtattgt agagattcag
    34501 ggtctcgggc gcaacggccc gcccccgcga ggataagtag gccatcgtgt tctccaccac
    34561 ggccgccagc atctcctccg gcggcaggga aagggcgcgg gcgcggaagg cgggatcgac
    34621 gtcgcgcctc ccgggctcga tcttgtccgc gcagtagacg aaggtctcga ggaggcccat
    34681 gcccgcccgc ccgagggtgt gccaggcgac ggaggcgagg agctccgcgt cctcgacgcc
    34741 gtagtcctcc ctgaggagct cggccgccgc gggaccgtgg aggatcttct ccgagaggaa
    34801 gcccgactcg agggcatagc ccgaggccgc ggccagggcc gcctggcgct ccggcgaaag
    34861 ctccttgcag aggtcgtggg cgaggccggc cgcccggccc cgctccggat cgaggccggc
    34921 acgggcgcag agggaggcag ccagcgccgc gaccccccgc gaatgcgccg cgcgcgcggg
    34981 cgacagctcg gcgtcgagcc tctcctcgag gagggccgcc agcctagcgg gctccataga
    35041 ggcccttcgc gtagatatac tcccggaccg cctcggggac gaggtagcgg aggctcctcc
    35101 cccgggcggc gcgctccctg agctccgagc tcgagagggg gatgaggcgg ttggaggcga
    35161 ggcggcaagg atagggaagg tcgaaggctc cgccgcccct gcgcgcgacg acgaggtcgg
    35221 cctccctggc gagggcctcg ggctcgcgcc agtccttgaa gccgggcgcg aggtcgtcgc
    35281 ccagggcgag gccgggcttt ccctccaggg ggtagcgcgc cttgaggccg cgcaccgtgt
    35341 cgatggtgta ggaggctcct ggcctttcga gctcgcagcc gtccacggcc agacaggggt
    35401 tgccggcgac ggccagggcc agcatctcga gccgctcctc ggggctcgcg ccgcctgagg
    35461 gtagctcctt gtgcgggggc ctggccgccg gcacgaggag gacaaggtcg taggagaact
    35521 cctcgcggag ctcctccgcg aggacgaggt ggccccagtg gacgggattg aagctcccgc
    35581 ccaggaggag gagcctcatt ccccttcccc tgcctcgccg aagaggtcgg cgtccgcccc
    35641 ctcctcgtcc agggcaccgg cggccccgcc cgtggcttcg ccgaagccgg gcccgccctc
    35701 ggcgaagagg cgggaggcct cggcggcgcg ggcctccgcc tccgcctcgt tctcgaggac
    35761 gagtcggagg aaggtctcct tgagctcggg caggcccgcg ccggagaaga cggagatgcc
    35821 gtagacgctc tcttcgggga aggcggcctt gaattcgtcg aagcgctccg ctgcatcggg
    35881 gaggtcgagc ttcgtggcga cgaggaggcg ccgcttcttc gcgagctccg gcgagaagga
    35941 ggagagctcg tccatgagga tgccgaaggc ctcgcgccag ttctcgtcgg agaggtccac
    36001 gaggaaggcg agggccgcgg tgcgggagat gtgcttgagg aagcggatgc cgaggcccgc
    36061 gccgtcgctc gcgccctcga tgatgccggg gatatcggcg aggacgatgt cccgctcgtg
    36121 gacggtgagc atgccgaggt tggggatctt cgtcgtgaag ggatagggcg cgatcttcgg
    36181 cctggcgttc gtgaagaagt cgaggaggga ggacttcccc gcgttcggga agccgacgaa
    36241 gccgatgtcg gcgatgagct ggagctcgac gcgcaggttg acggtctcag cgggcttgcc
    36301 gggctggaac tgcctgggcg cctggttcac gctcgacttg aagtgggtgt tgccccagcc
    36361 gccgttgccg cccttgagga agagccagcg cccttcgccc tcgcccgccc tggcggcgga
    36421 cttggccccg cccagccgcc ctcccccgtc gacggcggtc tccgtgccga agtccttgag
    36481 gacctcgccc gtctccgcgt tcttgatgag ggtgccggga ggcacgggga tgaccacgtc
    36541 gtcgcccttc ctgccgtgca tgttcttgcc catgcccggc tggccgttcc cggccttgaa
    36601 gtgctgctgg aagcggaggt gcgcgagggt gcggaggttg cggcggacct cgaagaccac
    36661 gtcgccgccc cggccgccgt cgccgccggc cgggccgccc atggggatgt acttctctcg
    36721 gcggaaggcg acgcagccgt cgcctccctt gccggaagag acggtgatgc tggcctcgtc
    36781 ggcgaacttg atcaaggtga cctgcccgat cgcggcggcc tacagcggcc ggccgcggaa
    36841 aatgcgtgac gaaacgaaaa ggccgcctcc gggcgaacgc gccgaggcgg ccttcaagga
    36901 aacgcgaaag gaatcgcctc gggggattcc ctaggccttc gcgggagcgt cggaggccgt
    36961 ctcggcgagg acctcgacgc cggcgtactt cttgcctttg aattccttga agaagaccct
    37021 gccgtccacg agggcgaaga gggtgtcgtc cttgccgcag cccacgttga ggccggggtg
    37081 gatcttggtg ccgcgctggc ggacgatgat ggaaccggcc gtcacgacct ggttgccgta
    37141 ggccttgacg ccgagatgct gcgggttgga atcgcggccg tttacgccct tgtgtggcat
    37201 atcattccct ccaataacgt tctatcgtaa gaccgacctc gccgggaaat tcccgttcaa
    37261 ggcctgaaat tccagcgaga aggaagtcgg cgatgccggc ggccttccct tcggcgcccg
    37321 ccccgaggct ccgcacgcgg aagctcaagg agccaggctc ccgggccgag ccttccagct
    37381 cggcgcccgg cagggcggag agggcctcgt aggcgctccg ggccaggacc gagaaggcgg
    37441 cgcagactat gtcgcgcccc ttaggtcccc tgcccgagtg gccctccgcc tcgaagcgga
    37501 cgagccgccc ctcggcgtcg agcacggctt tcgccgctat caccgcctac cgtcccttca
    37561 aagcctgtcc ccgagacgcc ggaggcgccc cgagggcggc catcagccga cgatctcctc
    37621 gaccttgagg agggtgtagg actggcggtg gccctgggtc ctccggtagt ccttcttcgg
    37681 cttgtacttg aagacgacga tcttatcgcc cttgacctcg tcctcgacga cggccttgac
    37741 ggcggcgccg gcgacatagg gggatccgac cttgacggtg tccccggaga ggaggaggac
    37801 cttgtcgagg gagagggcgg agcccttctc cgcctcgagg cggtcgaccc tgagggtggc
    37861 gcccttctcg gcgcgatact gctttccggc gatttccacg actgcgtaca taaagctaac
    37921 ctcgatgaat tcgatacttg ggcgacaact tatacaccgc ggggggccat gctgtcaatc
    37981 gactcacatg gtctccagga aggggacgca gaccaggcgg caaccggctt ccagggtccg
    38041 cctcacggcc ctgacgagct ccaggcggct ggccgccagg cccgcgtcgg ggcagttgag
    38101 gacctggttg tcccggtacc aggccgagaa gccgacggcg agctcgtgga ggtaggccgc
    38161 gacgacggag ggatccatgg cctcggcggc ctggtccagg gcttccggat aggccgcgac
    38221 gagcttcagg agggcccagt cggcgtcccc ggagagcctt ccggcctcgg gagagccctt
    38281 ggcggcgttc ccctcccccg cctcgtgctt gcggatgatg gaggaggcgc gggcgcccat
    38341 gtactggatg taggggcccg tgtcccccgt gaaggagagg gaggcctcgg ggtcgtagag
    38401 catgtccttg gtcggggcgg tctggaggag gtagtagtgg agggccccga gggctatcct
    38461 ctcgcccacc tgcgcggcgt cgccgacggc gccctcgcgg cccttggcct cgatctcctt
    38521 cgcggcgagg gcggagagtt cgtccacgag gtcgtcggcg tcgacgacgg tgccctcgcg
    38581 ggtcttcatc ctgccagagg gaaggttcac catcccgtag gagaggtggt ggaggtcgct
    38641 cgcccagccg aagccgaggc gcctcaggac ctcgaagagg accttgaagt ggtactgctg
    38701 ctcgttgccg acgacgtaga ccaggcggtc gtagggccag ctctcgtggc gggagatggc
    38761 ggtgccgatg tcctgggtga tgtagaggct cgtcccgtcc ttgcgcagga ggaccttccg
    38821 gtccaggcct atgtcctcca ggttcaccca gacggagccg tcctcttcct tgtagaagac
    38881 gcccgcggcc agaccgcgca ggacctcgtc gcggccgagc tcgtaggtct cgctctcgaa
    38941 gtcgaaaagg tcgaaggaga tgccggtgcg ggcgtaggtg gccttgatgc cgccgacggc
    39001 ccagccgttc atcttcttcc agagctccct cgtctcgggg tcgcccgatt cccatttcag
    39061 gaggagctcc tgggcctcct cctcgacctt gggatccttc gccttgagct cgttgaagag
    39121 gacgtagtac ttgccgacga aatggtcgct cttgaggccc tcgtcctcgg gactgcggcc
    39181 gccgcccagg cgcatgtagg cgagcatgga cttgcagatg tggacgccgc ggtcgttgac
    39241 gtggttgatc ttccgcacct cggcgccggc ggcggagagg acgcgggaga gggattcgcc
    39301 gaggacgttg ttccgcaggt ggcccaggtg gaggggcttg ttggtgttcg ggctcgagaa
    39361 ctcgaccatc accttgcggc cggagaggct caaggtcgcg ccccaggagg cctggccggc
    39421 ctcggcgagg atgcgggcgg cctcgggggc gcgcgaaagg cgcacgttga ggtaggggcc
    39481 ctcggccacg gccgtcccca gggcctgcgc ctcgggatcc ttcgcgacga tcgcggccag
    39541 ggcctgggcg agcatgggcg ggcccgcgcg gaggagcttc gcgtagggga acatggggaa
    39601 gccgaagtcg ccgagctcgg gcttgggcgg gcgctcgagg acgacggact cgggggaggg
    39661 aggctcggcg ggcgagccct tttccgctat ataggccgcg agggcgcggg cgacgatggc
    39721 tttccaggcg gtcttggctt cgaccatggg aggtatcgct ccttcggggt gtcgtgtcgg
    39781 ggccgagcat agcccgggcc gggcccgagg cgcaaggccg cggaccggcc gggagagccc
    39841 gccggacggt ccgcgccgtc cgcggccgcg agaagcggcc gcgaagtcgc ggcgcctagg
    39901 gctgccccgt cctagaaggc cgaggcgtcc ttgatcgtga ggccgcccgt ctgctccgcc
    39961 cagatcgtga agcggtcgcc gcggcggacg ggccggtgga gggcggctcc gtagaggctc
    40021 cgcacgacgc ccgcgcccct cgcggccttc tcgtccctga ggagggcggc gatctcgccg
    40081 agccgctcgg cgtagcgatc gggctcgagg ccgtagatct cctcgggcgg caggcggtac
    40141 atgaggggca cgccgcccga gtcgaggagg tcgagggcct cgccttccgc cgccggcacg
    40201 gcctcgctga aggcccggta cgggagggcc aggttgaccg cgcgcccctc ggtgatctcg
    40261 gaataggcga agttgcagac gacggcgtcc acgtagagct cgcgccccgt gagctccacg
    40321 cgctgggagg cctcgcgggg ccgggcgcgg ccgccctcgt agaaggagaa ctccgcggtg
    40381 aagcgcccat cgccggactc gaggatcgcg acgtcggcca cgcgctggcg ccggtcgagg
    40441 ttccgcacgg ccgcctgcag ctcggctatc cgttcgcggt tcgcgacgta ctcgtcgagg
    40501 cccttgaggg agccggcggc ggccgaggcg aggacgagga cgacggcgag gcccgcgagg
    40561 agggggagtc tcgtgaagag ctcggctgcg aaggcggcga gcctagagag ggctatgccg
    40621 gccgcctccc ccgcgcgcag gttcctgccg gcagcggccg cgaggagacg gcgctccgag
    40681 agggccttcg ccacggaggc gacggcggcc gcgaggacga ggcccccgag gaggaggagg
    40741 cccgggaccg tcgtgaagag gaaggccgag gcgtcggcga ggccatagag caggggcttg
    40801 aaggcgtcca gctggtcgtt cacgttcaat cctccatgat ctcgatgccg cccttggcgc
    40861 ggcagacgat cttgtagact acgcccacgt cgaagcggga gagggtggag acctcgtgga
    40921 cggccgagcc gaagctccgc tcggcgagcc gcgcggcctc ggagccgccc gtccgccgtc
    40981 cgcctgccgg cccgccgccc ggcgtcccgc cggcctggcc ctcctctccg ggctcgaggc
    41041 ctcccgccgc gcggagggcc tcccggtaga gctccttcac cgcctcgcgc tcggccttgc
    41101 ccatggaccc ggagccgaag acgccgggaa agccctcgcg ctcgtagtag ccgaggaggg
    41161 agacgccctc ctgggccggc gtgagctcgg tgaagacccg ccgcgggaag gcgagccagt
    41221 cctcgcccag gggcgcgacg acgaagtcga agaagagttc gccgcccctg aagctccgct
    41281 ctaaggcggc cagctccttg ccgtccaggt cgtagagctt gaccctggcg gagatctcgt
    41341 cggaggagcg cgagaggacc atgaagcgca gcgggacgcg ctctgccgtg agccgcgcct
    41401 ccagggactc gagggcggct atcttttcgc gctggcgggc cgtccccagg ccgaggacga
    41461 gccaggcgcc gagggccgcg aggaggacga gggcgaggag ccaggcgagg aggcgaagcg
    41521 gcttcttcat gcgcgccccg ccagggcgag gaaggcctcc tcgccgatga tctccacgcc
    41581 ataggcctgg gccttccggt tcttgtccga gcccgaggcg gggtcgttcg tgacgaggta
    41641 ggagagcccc ttcgtgactg aggatttcgc ctcgccgccc agggacttca ccagggcctc
    41701 ggcctcgggc cgcttcatcg ccttgagctc gccagtgaag cagaagctct tcccggccag
    41761 ggggcctccc gaggccggcg cggcgacgcg gacggcgccg gtcgcgagga gcctgtccat
    41821 ctcgggccgc agggccgcga gcccctccac gagggccctc gcggtgatct ctgcgaagcc
    41881 ctccacctcg tcgagctcct cgaccgtcgc ggccctgagc ttctccaggc tgtcgaagcc
    41941 ggccttcgcc gccttctcgg ccatcgtctc gcctatgccc tcgatgtcga agccggcgac
    42001 gaactcggag agccggagct cggccttcgc ggccaggttc cgcaggatct tccgggcgag
    42061 ggtctcgccc atgcggtcgt agagggcgag ctcctcggcc gtgagcgagt agaggtcggc
    42121 tatctcgcgg accctgcccg cggcgtggag cttcccgagg atcaccgagc cgaagtcgcg
    42181 cacgtccagg acggagagcc atttctccag gcggtgcagg gccttcttgg ggcattcggg
    42241 gttggggcag tagagcctgg tgccctcgtc cacgagggcg gagccgcagg cgcaagccga
    42301 ggggacctcg atggccttgg ttcccggcgg gttctccacg agggactcga tcttcgggat
    42361 gatctccccg cgcttcgtga tgacgacgcg gctcccgacg cggaggccca ggccgctgat
    42421 gatgttcgtg ttgcagaggt tggcccgctg caccgtcgtc ccggccaggc gcaccggctc
    42481 gaccagggct atgggcgtgt agctcgcgcc cgactcgctc cattccacgg agaggaggct
    42541 cgtcaccgcc tcctccaggc tgaacttgaa ggctatctgg cggacgggcc tggcccgcgc
    42601 caggtcggcc tcgtcgatct cccgggcctt cacgacgagg ccgtcgatgt cgtaggggag
    42661 cgaagggcgc tccgccatca cgcgggcgcg gtgctccacc acctcctcgc cgcttccgca
    42721 gcggacgagg gggacggtct cgaagcccat gcgggagagc caggcgagct tctcgacctc
    42781 gtcggcgaag gggctggccc gctccgagcc gaagaggtcg ccgcccgagg ggacggtccc
    42841 ggccgcgtcg tagcagatga cgtccaggtc gcccgagccc gagccgtcct tgcgcttcat
    42901 gaggccgttg gccgcgttgc ggcagttggc cttgtcggcg tatttcgcgt catggacggc
    42961 tcggaacatg aggacctcgc cgcggacgcc tcccgtgaag gcctccggga gccgctcgac
    43021 gacgccgcgc atcctcctcg cgttgggcgt gatgtcgtcg cccaccgccc cgtcgcccct
    43081 cgtcaccgcg cggaccagag cgccctcctc gtactggagc tcgaggctcg cgccgtccag
    43141 cttgtgctgg acgaggtact cgggatggcc gaccttggcg caccaggcca ggaagtcctc
    43201 ggggttggag gccttctcct ggctgcccat ggggatgagg tgggccgcct tgggccagcc
    43261 gtcggccgag tccgcgccca ccgccccgag gacggggttg tccgggtcca ggtcggccag
    43321 ctcgtcccaa agggcgtcga attcggcgtc ggagatctcc ggctggccgt tgtagtagag
    43381 gtcctggtgg cgctttacga gccccgcgag ctcggcgatg cggtccttct tcttgggcat
    43441 ggctggtctc cgcggccatt gtaggcggag gcgaggcgcc gtgtcaaagg cgccggcccc
    43501 gccaaggccg cgccggccag cgccgagcag cgcggagcgc gcgggctcga ttgacccgcg
    43561 aagccccttt cggctagtct cgagccgaag atccgccgac cgatcgatcc gcgaccgccg
    43621 cggacggcgc gggtctcccg cccgccgcgc cggtcctcgc ggcgcccgaa cccgaagccc
    43681 cgcgaaggcc cgggatcggc ccttccccgc gcaaaggctt gaacaagctc ccatgaacac
    43741 ctcacccgcc cccgaccgac gcatgctcct cctcctgccg agcgccttcc tcacctccct
    43801 gggcatcggc gtcatcaacc taggcatgct cttcgtcgtc aaggacgaat accgggcgag
    43861 cccatcggcc gtaggctggt tcgccgccct ctgggcgatc acctatttcc tcggctgcgt
    43921 cgccctccgc cccctccacc agaggatagg agcccgcctc tcgatggcgg cgatgaacct
    43981 gggcaccgcc ctcctcctcc tcgcccacct cttcacgagg aacctcgcct cggccttcgc
    44041 cgtctacggc ctctacggct tcatcaccgc ccttttctgg cccaggctca tgggctggct
    44101 ctcctccgga ctcgagggcc aggcgctgag caaggcgacg agcgccttca gcttctcctg
    44161 gagctcggga ggcatcgtcg cgccctacct cgcggggctc ctcgccgaga aggggcgctt
    44221 cctccccgtc tacgtctcca tcggcgtctt cgcggcgacc ggccttttca tcgccctctc
    44281 gagccgcctc gtcgccaccc ctcccttcgc gcctactccc ctagcgccgg ccgcgaaggg
    44341 ctccggaggc cagaccggcg cggaggccga gggcggggcc cgggccgaag ccgccaccac
    44401 ggccgaccgg tcgacgcccc tccgcttccc cgcctggatc ggggtcttcc tcctctacgc
    44461 cctcctttcg gtcttcttca acatcttccc gcttttcgcg aaggacgagc tcgggctctc
    44521 cgagtcgagg ataggcctca tcctcctcat ccgcgccgtc tccacgacgg cgggcttctg
    44581 gatcctgggc cgcgtggaga cctggcactt caagaaggcc tacatcgtcg cgcccatcgc
    44641 gatagccctc ctcatcgacc tcgccttcat cggcgtgcgc tcgcccctcc tcttcggcgt
    44701 cctcctcgcc gtcgccggct tcttccacgc gatggcctac aacaactcga tcttctacgg
    44761 cgtctccggc gccctggacc gggacaagcg gatgaccatc cacgaggccc tcctcacggc
    44821 cggacagatc ctcggctccc tggccggcgg catgctctac cagcgcttct cctggggctc
    44881 gatcttcctc ttcctggcct tcgtcttcgt cgcgggcatg gccggccagc tccgttacct
    44941 ccggaggagg agctgatggc ccgggcgggt tctcccgcgg cgcggccatc cgcccgcctc
    45001 ggactcctcg ccctttccct cctggcctcg gccttcctct tcctctcgtc ctgctcgccc
    45061 cgcgagcgcc tgctccgtct tgagaccgcg tccgaagaag ccgccgtcct aaagggccac
    45121 ctggccgacc atcccctacc caagggctgg cgccttgtcg ggcccgcggc cgcgggagac
    45181 ggggcggccg cggcaagcgg agcggccgcg ggagtcgcgg ccgacgccgt cctgagcctc
    45241 gcctgggtcc gggtcgctca ttcggaagcc ggcgacgaag ccggatcgcg cggggtctcg
    45301 gcggcggggc ggcccagcct gggccgccgc tacctcgccg ccgcggccgg cctcgccgag
    45361 cccgtctact ccctgggcag cctcgaggcg gaggcccggg ggctcgagcc cctcgaaggc
    45421 atccgcctcc cccgacgggc cctggcggtc gaggggctct ggcccgggga agagggctat
    45481 ccctacgagg aggagctcat cctttccatc gggcctgctg ccgagccggc tgccgagcgg
    45541 acggcggcgg ccgcgcagac ggcggctgcc gagcggacgg cggctaccgc gccgtctgcc
    45601 gctgtcgcgc ccgcggccgg gccggcggcc gcgcccaagg ccgcgtacaa ggtcccgcgg
    45661 gagctcggcc gctggctctc gcggctcgcc gcccaagcgg aaagcccgac ggccggcggc
    45721 tcgcccctgc ccctggacac cgctccgcgc ggccccgcca ctcctggccg gggccgggcc
    45781 gagaggccct tcaccatcgg cgcggtcggc gatctcgcga tagccgactc cgacgcgccg
    45841 aggctgtcgg gcgggccctc gggcctggcg agcctcttcg gctcggccct ccccgccctc
    45901 tcccggcccg acctcctcgt cgggaacctg gagggcgtcg tctcccccct gggagagccc
    45961 aatccgcgca agcgcttccg cttccgtttc cctcccgaga cgccccgcgc cctcgcctcc
    46021 gccggcttcg acctcttcct cttcgccaac aaccacggtt tcgactatgg ctcggaggcc
    46081 ttcacggaca gcctggccgc cctggaggcg gcaggcacgc ccttcgtcgg cgccggacga
    46141 agcctggccg aggccgcccg cccctggcgc gccgccctcc ccgccgcgcc cggccgcggc
    46201 ctcgccttca tcggcttcgc gaggtacccg gacgagcgct acggcttcac cgcggaggag
    46261 gccgcggccg gcccttccaa ggcgggcgtc ctctccgacg aatccgaggc cctgcgcgcg
    46321 atcggcgagg ctgcggccgc gggcgatttc gtcgtggtcc tggcccatgg cggggaggag
    46381 taccgcggag tcccctcgcg tccgacgcgg gagctttacc gccgcttcgt cgacgcgggc
    46441 gcagacctgg tcctgggctc gcatccccac gtcctgcagg gcgcggaggc ctactcgggc
    46501 ggcctcatcg cctattccct cgggaacttc ctcttcaccg ccgacaagga accccccgag
    46561 gccttgccga gcgcgatgct cgaattcctc gtcgagggag gcgagatcag gggggtgagg
    46621 ctcgagcccg tcctcgccct ggaggagggc acccgcctcg accccgaccg cacaggcgcg
    46681 gaggcccgct tcgctgccct ctgcgccggc ctcgccgcct cgccctagcc tcgccgcctc
    46741 ccctagcctc gccgcctccc ctagcctcgc cgggccgcgc cggtgccgga aacaaaaagg
    46801 ccgccttggg gctttccccc ttggcggcct tcgtcgtggc ctggacaccc ttcttgggag
    46861 gccgggcaga gaggtctcag gtgccctgtt tgatgaaggc gtccttgaag cccatggcct
    46921 tcaccctaga caggaccacg ccgccttcgt cgcgggacag gggcccgacg tagaggcggt
    46981 agcgggcgcc gcccgaggcc gcctcctcta gcgcgagggg gtagtcgccc ttgaagccct
    47041 tgaccgcggc atggagggcc tcgttcgtcc cgtagacgcc gatctggatg tagaaggagc
    47101 ccttggccag gcgcgcgagc aagggcaggc ccgcgggcgg ggtgaggggg ccggagggga
    47161 cagaggcggc gaccggagcc ggcttggccg gaaccgtggc gacctgagcg ggcgccgccg
    47221 gctggacggg cgccgcgggc tggacgggcg gcctcgaggc ggtcggctct aggctgagcg
    47281 ccagatccac ctcggcttcg ggctcggcgg caggcgcggt aggcgctgcg gccacgacct
    47341 cgacctcggc ctcgtcctcg gcggagggct caggctcggc gagggcgacc tcggaagcct
    47401 ccccggcggg cagagggccc agggcttctt ccgaagcctc ggcgacatcg aggggctcag
    47461 cctcggcctc tgaaggaacc tctgcctcgg cgagttccgc ctggagcgag gactcgaggg
    47521 cggccggctt ttcgagggcc agggcctcgg ccttctccct ttccgccatg tcgatctcgc
    47581 cctccgcgag ggcgaagtcg aggggggagg cgggaggatt ctcgagacgg gcgaggctct
    47641 cctcgggcag ctcgtcgggg cggagctcgg gctcgtccag ggcggccagg gcgccgtcgc
    47701 ccgggggaag ggccgcggcc tcgagaccga ggacggcggg gccctcctcg ggagcgccca
    47761 cgagcgcggc ggcgccgcgc tcctcgggat cgggaagggc gagttccggc gaggcggcgg
    47821 tctcgggcct ggcgtcgacc ctgacgatgg cctcgggcag gaccgagggc tcggcgcggg
    47881 ccgtgggggc ggcggagggc ggcgtggcga cgggcaacgc cggggccggc tcgaggggag
    47941 ccttggcttc ggcggccggg gccgcggcga cggtcgcctg agggacgaca ggagcggcgg
    48001 cgggagcggc gggctccgcc ttggcgaggg ccggctcggg cacctcgggc gcgagggtct
    48061 tggccgcggg cgccttctgc gtggtcggct ccacgacgct tgccgtctcc gccttctcag
    48121 gcaggggcgg ctcggcgggc ttgaggagta gggcgacgtc tgccggagcg gcgctt
//
LOCUS       DAOOSJ010000002        32863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_984_length_32863_cov_7.896946, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000002 DAOOSJ010000000
VERSION     DAOOSJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 32863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32863
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_984_length_32863_cov_7.896946"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1322)
                     /locus_tag="PLG14_00210"
     CDS             complement(105..1322)
                     /locus_tag="PLG14_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47576.1"
                     /translation="MRGIYLFLRRVSGLIGLLFGGRRAREALKELKKLDTATYASRIV
                     SDGVLMNDIPSPSDGEVLRMSFIKDRLGEFGISNVFSDERGNTLALFPAFGTKRDYVL
                     LVAEVGDANYSPLGNAVRLSRTRASGQGLGERSFGAAALLVFAEFAQATGFHLDKNLL
                     VLFTLSSSVDEREEAFRHFLDGWGDRVSCGILVRGAGLGLVETRHVGSYRLSVGVQSE
                     DKAILSPGAAASAASILGTIAAQVGGLTWDEKQTAMVNIARMEAGVGYGHWATQGELD
                     MEIVAEDEKMLETIKSVVAGCIEKTASGTKAKIDTLVRFKRSVGDPKLNAPLVAELKS
                     ALAEVGAKPRTGIVSEKVSLLNERGIPAIAVGLTTGTASPEGDALDLEPIASGFRQLL
                     LVVEGSAAAAGRD"
     gene            1576..2241
                     /locus_tag="PLG14_00215"
     CDS             1576..2241
                     /locus_tag="PLG14_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47577.1"
                     /translation="MRAFVDFVAATWSGAPAIARGFAVLLAGWLGALVTKAVVRGLLA
                     LLRFDRFADKVGLGAFLEKGKVAHRPAKLLSVLAYWLAMVIAFIMASRTLDIAAVNRV
                     SDGMVEALPALFAGIFVTIIGIVIVTFLGNVIETIARNAAIGDARAVARIFRYIGFAA
                     IVILASDQLGLGKGLLSTFLVIGFAAVSLAFAIAFGLGSVDLARELMQNLLKSLNERS
                     RKE"
     gene            2391..4382
                     /locus_tag="PLG14_00220"
     CDS             2391..4382
                     /locus_tag="PLG14_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOX47578.1"
                     /translation="MSTVELLAPAGNPEALDAAIGEGADAVYLGLRSFNARMRSSNFA
                     FNQFEAAVEVCRKNQKKVYVTVNTVFEQREADRMYQLLQYLERVGPDGLIVQDLGVVK
                     MAKDFFPSLKLHASTQMNIGSARGANFLSRQGFKRVVLSRELSLDEIRKVSETTNLET
                     EVFVHGALCVSASGLCLFSSYLGGKSANRGLCAQACRRLYSTEAGSGYYFSPDDLQLI
                     DRVPDLVEAGVDSFKIEGRMKSAEYVGAVVAAYRHMLDNWRIDRERALAKALSLLQAD
                     FARRKTSFNISGELDLDYLKPDQPGGTGIPLGKIRDIRSYEDGRWALMESAGAEGLGE
                     GDSLRVHHADDSGRFTARLKGVKEGPKGTLLRFDGDYRQGDEVYLVQTKTMGKRWKPV
                     LPKDIGRFHKFPSRDLAPKPDYPKLGKAELEVLPDGLYALVGRVADLHVLLAARPEKA
                     MLVFDRKNAEGLRRHEKEIPFKRDSLVLWLDPYLPEADSDWLSGELDYWIGQGQRFFV
                     ANNPAHLGLLRGRTDPKGRPVFTIAGPWLYVFNRYAAAFLLEEGARCVVPPYEIGKQN
                     FMKVAETVPSKAWMPVVFSYPPLFRIRTDLAKKYDFRYFSDRERQAYELVTGRDYSTV
                     IPQSPFSIVDRIPFLKRRASASSSSTSPSSSYRSRSTSR"
     gene            4702..5550
                     /locus_tag="PLG14_00225"
     CDS             4702..5550
                     /locus_tag="PLG14_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOX47579.1"
                     /translation="MSESAGKAIDLLFYLARTEGEVSLARLSRDTGMNKATALRYLSV
                     LESRGVAERRGGGWSLGLSLYELGTRVPVRSLVVERVRPHLERLARETGETANLACLA
                     GGSAVYLDRAEADRSLRMRSAPGDRLPLYCTGVGKAILSILPEDRARAILGSGLLPKI
                     NDKTVIDPEEIMREASKARELGYGLDREEYETGLTCMAVPIGLPEADFYGAMSVSGPT
                     ARMRDPAVRDKMIDALRRAAALAMSALRRDSEAADRIPLPLAGGGPTVEYAFRPSSGF
                     GADASE"
     gene            complement(5668..6045)
                     /locus_tag="PLG14_00230"
     CDS             complement(5668..6045)
                     /locus_tag="PLG14_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOX47580.1"
                     /translation="MNSLNSILLEGNLVRDPESKTLPTGSQVCNFAVASNRFYKQNEE
                     LEKEVSFFDVEAWSRLAQACAENLKKGRGVRVVGRLKQDRWTDPEGKSRSKVKIVAEH
                     VEFKPVFRKEGAEAEEKEAEPAF"
     gene            6220..7059
                     /locus_tag="PLG14_00235"
     CDS             6220..7059
                     /locus_tag="PLG14_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOX47581.1"
                     /translation="MSDQETNEARSRGRKEDFILRFEELILSGRFKAGERLPPERELA
                     ESLGASRPVVHAALGELAARGLVRIESRRGVFVADWRREGSVEMLLSLMSYGGGELSP
                     RLFDSVLEMRIHFETETARLAALRRSPEELEALSRVVARERLLDSREPRDVTALDYEF
                     HLAVALASGNDIYPLLMNSFRRIYTRILERFYGDPGVVPEVFRLHRELESAIASREEA
                     RAAAVMREILVYGERNLRRILVGEAGTAGGPAGGGAVGSGSAEGGGAAGGPAGSGAAG
                     GRA"
     gene            7056..8822
                     /locus_tag="PLG14_00240"
     CDS             7056..8822
                     /locus_tag="PLG14_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47582.1"
                     /translation="MSLSKLKAGVAKRAGSGAGALGRLDVEVREDRGSVVLEGRVPTW
                     ELKMELGWYAAGFGYKGVVNDVEVPDGKGGLLGDERLEAPPPAAGGDRPLEGAAFDAV
                     VVGGGVVGCAIARELARWRISVLLLEKEEDVAVHASSRNDGMIHDGFAARPGTKKAAY
                     NVRGNRLWEPLARELGIAFERPGSLIVYRSAASRAAYPLMAARAAENGVDGWEYWSRK
                     RLLAEEPNAADDQHGGFFLPSAGIVSPYRATVALAESAVLNGVRVALGACALGFGMED
                     GRIASVRTNLGDVRAGVVVNAAGGWADAVAGFAGDRFFSLHQRRGVDMILDQGAGAFL
                     NHIMSRPALAVANRRTKGGGLVKTIEGNVLVGPTASEAPGRESYPTTVAELRELERHF
                     KLNKRLSPSMAITYFAGVRACTYEEDFVVERSARVRNLVHAAGIQSPGLASAPAIAED
                     VARLAVEALSETRRVVPNPSFDPVRKASPELRRLPLEERAALIARNPSYGRIVCRCEE
                     VSEGEIRDALRSPLPVGSLDSVKRRARPGMGRCHGGFCTPRVMEIVADELGMDLCEVT
                     KKGPGSRVALGETKAGREGGAR"
     gene            8819..10222
                     /locus_tag="PLG14_00245"
     CDS             8819..10222
                     /locus_tag="PLG14_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47583.1"
                     /translation="MIERERRDVVVLGGGPAGLAAAVAAAEAGADVLVVEREERLGGI
                     LKQCVHDGFGLVRFGEQLAGPEYARRYVDLAEARGVESRLETFVHSVRREGPGGAAGG
                     GGPAGAGRATDQGSAAGSGGAAGPGPLVLTCVSQDRGVFEIEAPSLVLATGCRERTDR
                     QVFIHGERPAGILTAGLAQYLVNVEGLLPGKRAVVLGSGDIGLIMARRLTLEGMEVEG
                     VYELKGEPSGLSRNVRQCLEDFGIPLHLSTTVTEVHGKRRVEAVTVASVDARGQAMAG
                     TERRVACDTLVLSVGLIPEDETALSLGLDLDPSTRGPRVDQAGASREEGVFVCGNALH
                     VNDLADYVSESGAAAGRAAAERALGGAASRPERRLAPVKAGPGFLYAVPQYIDLSRPG
                     PAVLYFRSNATLREGARLVARALGPGGEETGLLLKKSYAALRPPEMERIEIDVASIPE
                     GTASVAFELERKESLRG"
     gene            10215..10739
                     /locus_tag="PLG14_00250"
     CDS             10215..10739
                     /locus_tag="PLG14_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOX47584.1"
                     /translation="MAEQRRFVCVACPRGCALEVDVAEAPAGGREALAVRGNACKRGE
                     DYGRAEVVDPRRSLTSTVRTEGTRRRRLPVRTSGTVPLGRLVEAARSLDSVLARPPVK
                     CGQVLARDLLGLGVDLVATDDLEEEGAVPAAAAKPGAGVAKPGAAAAKPGAAAVKPAA
                     GAQPSAGAPRRGRS"
     gene            10741..12306
                     /locus_tag="PLG14_00255"
     CDS             10741..12306
                     /locus_tag="PLG14_00255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOX47585.1"
                     /translation="MEELLLSIDAGTQSLRALVFDREGRLLVKKKIEFEPYVPGTPGR
                     AEQDPEVYWRALQAACRDLKARRPELMAAVAGVGITSQRATMVCLDEAGVPLRPAILW
                     LDVRKARPPYRPGLGMRAALGLVGMNEAVMLTQEEGKCNWIMQNEPGTWEKTHKYVEV
                     SGFLTHRLTGSFADSTASLIGHLPFDYKRQAWCKPGALNARLFPVPREKLPDPVAPGE
                     VVARITPEASAATGIPAGIPLVAAGSDKGCETIGMGVVDPTKASLSFGTTATIQTTSP
                     RYFEPLRFMPAYPAPIPGRFNPEVEIFRGYWMITWFKHQFAYEEVREAEARGIVPEAM
                     LDELLRRTPAGGHGLVMQPYWTPMFKMPSAKGAIIGFGDVHDRAHVYRAIIEGLAYGL
                     KDGLRAMERAGRSKVTELAVSGGASQSDEICRITADVFNLPLFRGATYETSGLGAAMV
                     VAAGLGIHPSVEAAVRAMARRDRVFAPDPARAELYDKLYSRVYKKMYGALAPLYEEIR
                     DITNYPERLGRAD"
     gene            12323..14053
                     /locus_tag="PLG14_00260"
     CDS             12323..14053
                     /locus_tag="PLG14_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOX47586.1"
                     /translation="MAEREFSPAWFEGEPPAKSFRSILKWGDPRAFKHPNRRLYRLMK
                     ETFGLDDEYFRHPFETGMETVPESRPCALEARHVEALRSIVGAENVFTDVYRRLGVAY
                     GKTMLDAMRLRKGIIENIPDAVLHPRSRADLRAVVDYCAANGIPVYVYGGGSSVTRGV
                     EAVKGGVTLDMRVHLNKVLAFDEKNHTITVEAGMSGPELERQLRNAAALHGAKRAYTC
                     GHLPQSFEYSAVGGWVTTRGSGQNSSYYGNAADMVIAQDYVCPACDIRTGEFPAAANG
                     PDVDEIMMGSEGAFGVLYSATLRFRMYMPENTRRYSYIFPSWEEAKEAAREIMQGEFG
                     HPSVFRLSDPEESDVALKLYGVEGTVLDTLMRLRGFRKGERCLMLGTADGEAGFSRHV
                     ARMVGRVARRHGAMSTTGLVQRHWEEGRFRDPYMREDLQDYGIMMDTLECSVNWSNLE
                     TVHTGVREFCHSRPDTICMTHMSHFYPQGCNLYFIFIAKIDRIDEYLAYQSGILDSIQ
                     KHGATMSHHHGIGKAFAPWLEGDVGPEAISLFRAIKRHLDPKGIMNPGGTIALDLPDE
                     KRRILRKPGR"
     gene            complement(14196..14873)
                     /locus_tag="PLG14_00265"
     CDS             complement(14196..14873)
                     /locus_tag="PLG14_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX47587.1"
                     /translation="MRPASRVLTPLIRSLLGLLCRVDSKELAKVPAKGPLIVVINHVN
                     FLDGPLLYSYLASRRLVGVAKRETWDNPLLGFLADAWETIPLDREGADLGAMRRIMAA
                     LAAGRVVALAPEGTRSGHGRLQRGHGGVAQIALRSGAPVLPVAITGCERFWPNLRRLR
                     RTRVAFRVGESFRLKEPQGGATRRDRLDMTEELMNRLALLLPPRQRGAYPEPENSPGR
                     FLEFTGA"
     gene            complement(14870..16801)
                     /locus_tag="PLG14_00270"
     CDS             complement(14870..16801)
                     /locus_tag="PLG14_00270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOX47588.1"
                     /translation="MSETPRELLKRVFGYDNFRPNQERIIEAALAGRDVLAVMPTGGG
                     KSLCYQIPALASEGLALVVSPLISLMRDQVAFLRELGAPAIFLNSSLSPEEWRAGAAS
                     LRAGGARLLYAAPETILSPRGSELLSSLRVKLVAVDEAHCISEWGHDFRPEYRLLGSL
                     REAFPGAPFLALTATATPRVRADIARALALRDPLEIVASFDRPELFLEARPRGPNGGY
                     DEIASFAAGFPEESGIVYCFSRARAEEVARRLSERGIPALPYHAGLPDEERARNQDAF
                     IDDEVRVVAATTAFGMGIDKPDVRFVVHADLPKSLEQYYQEIGRAGRDGLPARCLLLY
                     GYGDAQKMRALLSGKEGDEALAAEAALSAMLRYAEKPGCRRLALLSHFGEAYAGPPIA
                     RPDAAAGCGACDACVPGAAGAGAAPETEVTTEAYKFLSCVKRTGERFGAGHVADVLVG
                     SRNERVLGLGHAELSTYGIGKEWTRAQWMDLSRQLARKGYLDRDEEFGVLRLTPLAYA
                     AFKDRSPILGTVPPRGRKDAPSGARAAAGAAGAGLASALASGPGADALAASLRALRRR
                     LAEEAGVPPYVVFADRTLYDLVSRRPSSLPGLLEVFGMGEVKAGRYGAAILAAVAAAE
                     GAPGGGSRTAVAAAEEDDE"
     gene            17076..17717
                     /locus_tag="PLG14_00275"
     CDS             17076..17717
                     /locus_tag="PLG14_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX47589.1"
                     /translation="MLEFIVKGGLVLWIIMTLSAVGVAIIVERLVYFRKIRVDEEKLF
                     LRIKASLEKGHFDEALAICDQNVSPLSGLMRVGIEHREYPETVQKEVLKDAAAQEVPR
                     LEKNVSALGTIAHIAPLLGLLGTVTGTMKAFGVLGRFGSVADPSVLARGVSEALITTV
                     GGIVVAVPAVIFYNYLVTKVNLILVQMENQVNALVLMINASRLKAASREGGKA"
     gene            17714..18160
                     /locus_tag="PLG14_00280"
     CDS             17714..18160
                     /locus_tag="PLG14_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX47590.1"
                     /translation="MRLERRLKPTINIDMTPLIDVVYQLVIFFMITSVFKMAPGIALE
                     LPGAATASSVAVTELRIVAVSEDEIYVGKERTSLAGLETLVRRAVEGTEPKDRRAVLE
                     GDKAMPYQLMVSILDALRENGVDGVGLQTSPGGGAGAKAAPAKASP"
     gene            18157..19131
                     /locus_tag="PLG14_00285"
     CDS             18157..19131
                     /locus_tag="PLG14_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47591.1"
                     /translation="MRREDWLRAQDRKRRVLAWAATAAAYLLALGLVLLVEGFRVEEV
                     SDYAGPVRVSLGSPEGNAARGLLESPPELSEPPAPAPAEPTPPEPAAAAPTSPATPAP
                     AAPAQPKAPAAAKPAPAAAKPAPAATASAAAAPSAPAAPTSPPPPEVARGTEAGNDYE
                     TSFGGDSGLIRRSLKVPIYRYLPPPFYLDQKYVDSIAGLPDAVNPMPEEARRRLLITY
                     YAKSGDVWKQKLPVPLDVRERLWVVLEEAGFDPRRADYKLNNPKPITLSFTMVPIKNQ
                     MPELRDLRIIESSGFPDLDEAVMYGFQKAGFSSDATKAVTGRFVYRFN"
     gene            complement(19124..20473)
                     /locus_tag="PLG14_00290"
     CDS             complement(19124..20473)
                     /locus_tag="PLG14_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX47592.1"
                     /translation="MAHWAEDSVFYHVYPLGYCGAPGRNDFSSAPIPRLERLTERLGR
                     VAELGADALYLGPVFESSAHGYDTVDFRLVDRRLGDSSTLARLSDGLHRRGMRLVLDA
                     VFNHVGRGHPWFRDVAERGRSSRFAAWFRGLDFSRPSPLGDPFSYEGWNGCLDLARLD
                     TGNPEVRAELVAASLSWIDDYGVDGLRLDAADLVDLEFQRELAAACRARRPDFWLMGE
                     VVHGDYRRWAAPGLLDSTTNYECWKGLWSSLKDGNYFEIAYALKRQFGEGGIYRGLHL
                     YAFADNHDVDRAASLLGDEALLFPLYCLLFTMPGPPSLYYGSERGAAGRKAGGSDAPL
                     RPSVEALEAGPEVPGLEAAVRRLASLRKAVPALRRGGYRELLVAPRQFAFERSCEAGP
                     AVVALNAEKRAVELEVPVPFPAAFLRDALDGGERFPVRNGKVRLTLHPTWARVLVPS"
     gene            complement(20644..21222)
                     /locus_tag="PLG14_00295"
     CDS             complement(20644..21222)
                     /locus_tag="PLG14_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX47593.1"
                     /translation="MEETGGNRELILAEAARLFSERGYEAVGVAEIAEASGVTKPTLY
                     HYFGSKRGLLDAAIEERGGPLLAAVRAAARYGRDVPKGLEEIALAFSRFALADPVFAR
                     MRLAMSFAPPGSEAGEAAASLNEALFEAVRSFFAAAEKDHGNMRGRSRAFAGTFIGTI
                     DSYAGFHLAGRSGLDERTIREAVRQFMYGIFS"
     gene            complement(21368..22123)
                     /locus_tag="PLG14_00300"
     CDS             complement(21368..22123)
                     /locus_tag="PLG14_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX47594.1"
                     /translation="MIDARLKDIDGGRVLDLATGSGAFAARLAGSLASYEEIVAVDSA
                     PRALEAARKNLAATRAARVEEADAAALPYADASFGLVGLSNSLHHFPDPARVLSEALR
                     VLAPGGRLVVLEMHRDSGEGPAMTHVLMHHWWAEVDSAGGVHHAETYGRAALRGLVEA
                     SAGAGGFAESSWDEIVEEEGDPLDPELLAEIEKIVETYSGKIEALGEGAGGARVPPEA
                     RAAMKARGLELLARARSVGFRSAPSLFFVGRKA"
     gene            22858..25455
                     /locus_tag="PLG14_00305"
     CDS             22858..25455
                     /locus_tag="PLG14_00305"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX47595.1"
                     /translation="MGSLAGSCPEAYAEFGPPRLRAGLVRRERLFGLLDLGAGRRLVV
                     VKSPGGFGKTTLLADWLQARRRRYSWLGVEEGPLEGRELLALVAASLRSSLGEAGKAL
                     PEEGEGAGLVRAIAAAVGALDEAFSLVIDDCHLIGDGRAGLLLLRLAEALPPNASLVL
                     SGRGEPSFPLARLRSRGELLEIGAAELRFTAAETRDFLRGPLGDSYSDDEAALLGAKT
                     EGWVAALQMAVLGLRGRADRRAFLESFGGAHRDVFDYFAEETFAALDPGLLAFLLPCS
                     VLETLSAPLCRELTGREDSAALVERLVDEGLFLLPLDDERRRYAFHGLFREALLRRLE
                     SDLPEALPKLRLAAAGWLAANGQPLEAMRLRLLAGDAEGAAALAEGRALSALESGRVE
                     DLRSAIAVLPREAVESRPWLAVAAAWAAVYAGRLSDAAAFADAAEVRASAQSRIIGHV
                     EAIRAYRANLEGDGEEAKLRALRAFELLPASDSMARAHAGKSLGFGFTLLHDYAAASR
                     ALEGAIEEAGSRGGHHIRVLAANDLAYLAMTQGRFSEAEEICRREIAAGSGGGREAPV
                     LGCLHSLLSNICLSRGEIPAALEQARLGLEMGLAWGNMDRLVFSYVMYATNLAAAGDR
                     PAARAALERLRALPDLTPRHAAVILMTAAEIDPAGPDAARMERMAPLKDENFNLSFQA
                     VRARALLAKRRAAECLAVVLPSLELARRWNLRPVQLDALVNAALAQRSLGRREEARAA
                     AAEARALAEELGDLLRIPARGPAIRSLLEEEGLLSGSFGARLAAAFDLFVERPAGEAD
                     FRAGEPLSPREREILSLLVQGRTAEEIASLLFVAPSTVRSHVKSLYAKLGVHRRIEAA
                     NKARELGIL"
     gene            25649..28216
                     /locus_tag="PLG14_00310"
     CDS             25649..28216
                     /locus_tag="PLG14_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX47596.1"
                     /translation="MKRFAVSVLVVALAAAGLSAQAKGPIVDKILLDAKTQEDIALKD
                     VASGRSDLFTYDVSAATFKALPDDIRNRLDPYAITGASYNTLFTNPFPNKAPYQAKRD
                     GKVTLNPLAIREVRFALNYLISRKQIIDEILSGGGLPMYTPVIPGQPNSSRYGLVASK
                     LGFTAGGNEKKAIADIGSALEKAAALPENKGRLVRGDRYWTFDGEPLLVKFLIRVDDP
                     SVRLPMGRYIASQIEKAGIKVERLEWDRTKCRETYDQNDPADLGWNLYTEGWGGGQTL
                     AYWDQSIAQMYAPWMGDMPGGNRAGAWSYEHPELDKLTSDSVNGRVKDEADYYAKCLK
                     ATELGLKEAVRLYVVATTNYTCASKDRFNNRMVYGLGDGLNNFSYYTADVKPDKDGQK
                     VLRISGFSSRGDLFNSAWDPIGPDGFSDTYSGTVARQLSDNEYNANPVTGINIPMMAS
                     WSKVKTDIAVSADGSLQGRLAIPAEAVFYNALTQKWDSSVVYVDRKGDGSEYGYAKRS
                     ENPAFSKAFSTATFAFKPGKWHHGRAMGIVDYRYAIARQVELCVQRGADDKVYEESYA
                     ASWNPNLVRFKGYVFNKDGTLTVYGDANYPMDQDQLATLLCPQLMIEASNYGDVLPWE
                     IHEGLKYLVAEGSASKTLYRFNSDSNFTEVDLISQRCVADLKAKLQELKARKHVPVSI
                     AAWVTPEEAAKAYDQAIAFIDKHGHAMMSNGGFILDRYDAANKTAILVANRDPAYPFE
                     KGHFAKALASSYARIESVKVPAYKKGADLVVSATVSEVAFPSNGAKPAAKLTAKVTLV
                     ADKDYVFAAKLAKAGTIEAAIPAKVLGTLKAGSYTLVVEAALGNEAPTVETANVLVF"
     gene            28332..29372
                     /locus_tag="PLG14_00315"
     CDS             28332..29372
                     /locus_tag="PLG14_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX47597.1"
                     /translation="MYGKYVLKRAGYGILMYVIMIFVYSTLFNSVADRTMSSLIDEQV
                     AQEMTRYKSLDEAQRAGLREERIQAKRRQYHLDEPLASRIFWRGVDTITFNFRNSTII
                     KASDGDRRVLKIVGEALPRTIVLFTTEAALVTVVGVMMGLGLARKPNGPLDRFNSGIT
                     MITNGLPAWWLGMLMIMVFSYALPVFPSGGIRSNPPPEGLMGVLDYLWHLALPLVTLT
                     LLGLWSTAYITRNIVLSNLQEDYIMAARARGVPERLVLFSHTLRSSMPAIMTLAILGL
                     FGSIAGNIIVEGIFGWPGIGNLYFVAVQQNDVPVLMATLAIETLINMGGFVLLDIVYG
                     LLDPRIKTGGRA"
     gene            29369..30772
                     /locus_tag="PLG14_00320"
     CDS             29369..30772
                     /locus_tag="PLG14_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX47598.1"
                     /translation="MNLRDSLEGLGAFWKEFSKNKTGLVGLGLLAVFVLLGLFGTLVV
                     PFPGAIDHWRDIGFWQDNPQAAPPVWTNWFAKRKGVVSSVLDRAQAEESTLDSGARVS
                     SLTFDYDFGYDLPPRDVVVRFEGSGLVPVTITLERPDGLVAELYRAQLELSEGDVQRV
                     SLARNCSQEVVEFLRASDEALAASLDADLVKPVSLLFSRLSPGMAESPEALKGAYRLK
                     VNAFLFSEDARLAKASAVVSGHVSGIFGTDMAKRDVFTGLVIGIRWALVIGLLTSIVT
                     VMAGVLLGVTAAYMGGATDWLLSRLYELIFLMPVLPFLIVVSAIFKPTIWTLILIICL
                     FFWAGPFKPVYSMALQIKEETFVEASRALGSRSWRIILRHIIPILLPYSFASMALAIP
                     SVIVYEASVSLLGLGDSSIVTWGQLLNDALGQGAVINQLWWWVVPPGLMIALMGMSFA
                     FLGTALDKILHPKLKTR"
     gene            30777..31802
                     /locus_tag="PLG14_00325"
     CDS             30777..31802
                     /locus_tag="PLG14_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX47599.1"
                     /translation="MSDNKDKNAKEPVLEVKDLRLHYFTSKGVVKALDGVSFRLDPGE
                     ALGLVGESGCGKTTTGVALLNMPSPPGRIVSGSIKVGGVEIVGMEEQELRRTVRWSRI
                     SMVFQGAMNCLTPVYTIRDLMLETWRVHDDAERPDLPKVEALMRKYLALVGLPEHVLG
                     RYPHELSGGMKQRVVIAMALFLEPQVIVMDEPTTALDVIVQAQILNLIKDLKAERGIS
                     YIFITHDLAVEAEVADRIMVMYAGKAAEIGTNEQIFGEAGPAHPYTRKLLASTPRIKS
                     RVSELAFIPGSPPDLVDPPPGCRFAPRCEFAKPKCHEAEPPFGEIESGHFAACWKAME
                     DPEYERN"
     gene            31789..32817
                     /locus_tag="PLG14_00330"
     CDS             31789..32817
                     /locus_tag="PLG14_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX47600.1"
                     /translation="MSAIETGAAPAAAAPAADASAVLELQDLRREFRVHRGKSVVRAV
                     DGVSLKVARGEILGLVGESGCGKTTIGKLVMKLIAPTGGRILRKGEDCTDLDRAATEA
                     YRRRVQMIFQDPYASMNPHYKIRDVLEEPLLIHRVGGSRAERAALAEEVMAKVKMTPA
                     AEFLDRHPHMLSGGQRQRIATARTLILKPELIVADEPVSMIDLSTRAEILHLLKTLQG
                     DMELSFLYITHDISTARYFTDRIAVMYLGRIVEIGAQDEVIDHPRHPYTRALIEAVCE
                     PVPGRVGVKRALNVTGEIPSATAVPSGCRFHTRCCYAKEECASLPEPELQDSGGGHLC
                     ACRRWKEI"
BASE COUNT         4973 a        12021 c        11112 g         4757 t
ORIGIN      
        1 ctgcttgagc tcgacgaggc gcttcttgtc cttgaaggcg ctgttgtggt aggagcgcga
       61 gaggatcgac ggccggtcgt tcacggcttt cgtctcggcc acggctagtc cctccccgcg
      121 gcggcggctg agccctctac gacgaggagg agctgcctga agcccgaggc gatgggctcg
      181 aggtccagcg cgtccccctc gggagaggcc gtccccgtgg tcaggccgac ggcgatcgcc
      241 gggatgccgc gctcgttgag cagggagacc ttctccgaga cgatgccggt cctgggcttg
      301 gccccgacct ccgcgagcgc cgacttgagc tcggccacga gcggcgcgtt gagcttcgga
      361 tccccgaccg accgcttgaa cctgaccagg gtgtcgatct tggccttcgt gccgctcgcg
      421 gtcttctcga tgcagcccgc caccacgctc ttgatcgtct ccagcatctt ctcgtcctcc
      481 gcgacgatct ccatgtcaag ctcgccttgc gtcgcccaat ggccgtagcc cacgcccgcc
      541 tccatgcggg cgatgttcac catcgcggtc tgtttctcgt cccaggtgag gccgccgacc
      601 tgcgcggcta tcgttcccag gatggaggcc gcggaggccg cggcgcccgg cgagaggatc
      661 gccttgtcct cgctctgcac gcccaccgag agccggtagg agcccacgtg cctggtctcg
      721 acgaggccga ggccggcgcc gcggaccagg atgccgcagg agacccggtc gccccagccg
      781 tccaggaagt ggcggaaggc ctcctcgcgc tcgtcgaccg acgaggagag ggtgaagagg
      841 acgaggaggt tcttgtccag gtggaatccg gtcgcctgcg cgaattcggc gaagacgaga
      901 agggcggccg cgccgaagct ccgctccccg agcccctggc ccgaggcccg cgtccgggag
      961 agccgcacgg cgttgcctag gggcgagtag ttggcgtcgc cgacctccgc gacgaggagg
     1021 acgtagtccc gcttcgtgcc gaaggcgggg aagagggcca gggtgttgcc ccgctcgtcg
     1081 gagaagacgt tcgagatgcc gaactcgcca agccggtcct tgatgaagct catgcggagc
     1141 acctcgccgt cgctgggcga ggggatgtcg ttcatgagca ccccgtccga gacgatcctg
     1201 ctcgcgtagg tcgcggtgtc gagcttcttg agctctttca aggcctcccg cgcccggcgc
     1261 ccgccgaaaa gaaggccgat gagtccggag accctgcgca ggaacaggta gattcccctc
     1321 atgccgtttc actctcccgc cgcgaagatt ggttttcctc ccgctccggc cctccgccgg
     1381 cgccttcggc cttgcccggg gtcgatccgg gcgccgcccg gttcaagtat agccgaccct
     1441 ccgcccttgg gcgagggccg ggctggcggt ccggccccgt cgatggtagc atgggagtac
     1501 ggcggccgag ggccgcggcg cgaaggccga ggcaggcggc gccgcgctcc gtcgcgccgc
     1561 ggaaaggagg ctccgatgcg ggcattcgtc gatttcgtgg cggccacctg gtcgggcgcg
     1621 ccggccatag cgcgtggctt cgcggtcctt ttggccggat ggctcggcgc cctcgtaacg
     1681 aaggccgtcg tgcggggcct cctcgccctc ctgcgcttcg accgcttcgc cgacaaggtc
     1741 ggcctgggcg ccttcctgga gaaggggaag gtggcgcaca ggcccgccaa gctcctgagc
     1801 gtcctcgcgt attggctggc catggtcatc gccttcatca tggcctcccg caccctggac
     1861 atcgcggcgg tgaaccgcgt ctccgacggc atggtggagg ccctgcccgc cctcttcgcg
     1921 ggcatcttcg tcaccatcat cgggatcgtg atcgtcacct tcctgggcaa cgtgatcgag
     1981 acgatagcgc ggaacgcggc catcggcgac gcccgcgccg tggcgaggat cttccgctac
     2041 atcggcttcg ccgccatcgt catcctcgcc tcggaccagc tcggcctggg caaggggctc
     2101 ctctccacct tcctcgtcat cggcttcgcc gccgtttccc tggccttcgc gatcgccttc
     2161 ggcctcggct cggtggattt ggcccgggag ctgatgcaga atctcctgaa aagcctcaac
     2221 gagcggtcgc gcaaggagtg agcccgcggg cgcgtcccgc cctgtaatgg gcgccggcct
     2281 gtcttggagc gcttgccggg tcttgcgccc gagtcggcgc gaaaaggccg gaagacgatg
     2341 cggttttccg catacttaca agaacgctca aaatttgata gtattgaaac atgtccactg
     2401 tcgaactgct cgcccccgcg ggcaaccccg aagcccttga cgccgccata ggcgagggcg
     2461 ccgacgccgt ctacctcggc ctccgcagct tcaacgcgcg gatgcgctcc tccaatttcg
     2521 ccttcaacca gttcgaggcg gccgtcgagg tctgccggaa gaaccagaag aaggtctacg
     2581 tcacggtcaa cacggtcttc gagcagcgcg aggccgaccg gatgtaccag ctcctccagt
     2641 acctggagcg ggtaggcccc gacggcctca tcgtccagga cctgggcgtg gtcaagatgg
     2701 cgaaggactt cttcccctcc ctgaagctcc acgcctccac ccagatgaac atcggctcgg
     2761 cccgaggcgc gaacttcctc tcccgccagg gcttcaagcg cgtcgtcctc tcccgcgagc
     2821 tctccctgga cgagatccgc aaggtctcgg agacgacgaa cctggagacc gaggtcttcg
     2881 tccacggagc cctctgcgtg agcgcctccg gcctctgcct cttctcgagc tacctgggcg
     2941 gcaagagcgc caaccgcgga ctctgcgccc aggcctgccg ccgcctctac tcgacggagg
     3001 ccggctccgg ctactacttc tcccccgacg atcttcagct gatagaccgg gtccccgacc
     3061 tcgtggaggc cggcgtcgac tccttcaaga tcgagggccg gatgaagagc gccgagtacg
     3121 tcggcgccgt cgtcgcggcc taccgccaca tgctcgacaa ctggcgcatc gaccgcgagc
     3181 gcgccctcgc gaaggccctc tccctcctcc aggccgactt cgcccgcagg aagacgagct
     3241 tcaacatctc gggcgagctc gacctcgact acctcaagcc cgaccagccg ggcggcacgg
     3301 gcatccccct cgggaagatc cgcgacatcc ggagctacga ggacgggcgc tgggccctca
     3361 tggagagcgc cggggccgag ggcctcgggg agggcgactc cctgcgcgtc caccacgccg
     3421 acgactcggg ccgcttcacc gcgcgcctga agggcgtgaa ggaaggcccc aaggggaccc
     3481 tcctgcgctt cgacggggac taccgccagg gcgacgaggt ctacctcgtc cagacgaaga
     3541 ccatgggcaa gcgctggaag cccgtcctcc cgaaggacat cggccgcttc cacaagttcc
     3601 cgagccgcga cctggccccc aagcccgact acccgaagct cggcaaggcc gagctcgagg
     3661 tcctgcccga cggcctctac gccctggtcg gccgcgtagc cgacctccac gtcctcctcg
     3721 cggccaggcc cgagaaggcc atgctggtct tcgaccggaa gaacgcggag ggcctccggc
     3781 gccacgagaa ggagatcccc ttcaagcgcg actccctcgt cctctggctc gacccctacc
     3841 tccccgaggc cgactcggac tggctctcag gcgagcttga ctactggatc ggccagggcc
     3901 agcgcttctt cgtcgccaac aaccccgccc acctcggcct gctccggggc cggaccgatc
     3961 ccaagggcag gcccgtcttc acgatcgcgg ggccctggct ctacgtattc aaccgctacg
     4021 ccgcggcctt cctcctggag gagggggcgc gctgcgtcgt cccgccctac gagatcggca
     4081 agcagaattt catgaaggtc gcggagaccg tgccctccaa ggcctggatg ccggtcgttt
     4141 tctcctatcc gcccctcttc cggatacgga ccgacctggc gaagaagtac gacttccgct
     4201 acttctccga ccgcgagcgc caggcctacg agctcgtcac gggccgcgac tactcgacgg
     4261 tcatccccca gtcgcccttc tccatcgtcg accgcatccc cttcctcaaa aggagggcat
     4321 cggcaagttc atcatcgact tctcccagct cgagctaccg aagccgctct acaagcaggt
     4381 gatgaaggcc gcggaggagg gcagggtcct ccccgagacc ggccgcttca actggaagga
     4441 cggcttctgg cagccgcccg aggctccttc cgtcgactga gcgcggccgc cgggtcgccg
     4501 ggcgcctcgt gtatcgcggc cgcctggcgg ctccttgggc ggcggccgcc attgttccga
     4561 cgccggccgg cggttcagcc cccgatagcg catttcgact ttcacgcttg acggcttcgg
     4621 actcgggtgc taacctagct ccaatagttt gttcggcaaa acatcgtttc gtccgtcaaa
     4681 ccgactagga gcatcgccac aatgagcgaa tcagccggaa aagccataga ccttctcttc
     4741 tatctcgcgc ggaccgaggg cgaggtcagc ctcgcgcggc tttcgcggga cacgggcatg
     4801 aacaaggcga cggcgctccg ctacctttcc gtcctcgagt ccaggggcgt cgccgagcgg
     4861 cggggcggcg gctggtccct cggcctctcc ctctacgagc tgggcacccg cgtgcccgtc
     4921 cgctccctgg tcgtggagcg cgtgcggccc cacctggagc gcctggcccg cgagaccggc
     4981 gagaccgcga acctggcctg cctggccgga ggctccgccg tctacctgga cagggccgag
     5041 gccgaccgct ccctgcgcat gcgctccgcc cccggcgacc gcctgcccct ctactgcacc
     5101 ggcgtcggca aggccatcct ctcgatcctc ccggaggacc gcgcccgcgc catcctgggc
     5161 tcgggcctcc ttcccaagat caacgacaag accgtcatag accccgagga gatcatgcgg
     5221 gaggcctcga aggcccgcga gctcggctac ggcctggacc gcgaggagta cgagacgggc
     5281 ctcacctgca tggccgtgcc cataggcctc cccgaggcgg acttctacgg cgcgatgagc
     5341 gtctccgggc cgacggcccg catgcgcgat cccgccgtca gggacaagat gatcgacgcg
     5401 ctcaggcgcg cggccgccct cgcgatgtcg gccctgaggc gcgactcgga ggccgccgac
     5461 cgcatccccc tgcccctggc gggcggcgga ccgaccgtgg agtacgcctt ccggccctcc
     5521 tcgggcttcg gggccgacgc ctcggaatag ggccaggccg gccgggtcgg cgcgaaggct
     5581 tccgcccaca cgtcaaaaag ggggatgggc aggcggaatc cctgcgcgcc gtcccggccg
     5641 gcttcctctt ttccgcggcg cctggcttca gaacgccggc tcggcttcct tctcctccgc
     5701 ctccgcccct tccttcctga acaccggctt gaactccacg tgctcggcga cgatcttcac
     5761 cttcgagcgc gacttgccct cgggatccgt ccagcggtcc tgcttcagcc ggcccacgac
     5821 ccgcacgccc cggcccttct tgaggttttc cgcgcaggcc tgcgccagcc gcgaccaggc
     5881 ctccacgtcg aagaaggaca cctccttctc gagctcctcg ttctgcttgt agaagcggtt
     5941 cgaggcgacg gcgaagttgc agacctggct gcccgtcgga agggtcttgc tctcggggtc
     6001 gcgtacgagg ttgccctcga gcagtatgga attgagagag ttcatcttgc ctccttggct
     6061 ggggcgccgg aacctgcccg ggcgtttccg cgccggcgcc ccgcatagct actacgcccg
     6121 gccggaacga aagcgattga aagtctcttc ttgtgcttga cgggagacgg actcggatct
     6181 atgctctggt aataccacag agaaggctgg atataccaag tgtcggatca agagacgaac
     6241 gaagcgcgaa gccgcgggcg caaggaagac ttcattctcc gcttcgagga gctcatcctc
     6301 tcgggccgct tcaaggccgg cgagcgcctt ccccccgagc gcgagctcgc ggagagcctc
     6361 ggcgcctccc ggcccgtcgt ccacgccgcc ctgggcgagc tcgccgcgcg gggcctggtc
     6421 cggatagaga gcaggcgggg cgtcttcgtg gccgactggc gccgcgaggg ctccgtggag
     6481 atgctcctct ccctcatgag ctacggggga ggggagctct cgccccgcct cttcgactcg
     6541 gtgctggaga tgcgcatcca tttcgagacc gagacggccc gcctggccgc cctccgtcgc
     6601 agtcccgagg agctcgaggc cctctcccgc gtcgtcgcgc gcgagcgcct cctcgactcc
     6661 cgcgagcccc gcgacgtgac ggccctcgac tacgagttcc acctcgccgt cgccctggcc
     6721 tcgggcaacg acatctaccc cctcctcatg aactccttca ggcgcatcta cacgcggatc
     6781 ctggagcgct tctacggtga cccgggcgtc gtgcccgagg tcttccgcct gcaccgcgag
     6841 ctagaaagcg ccatcgcctc ccgcgaggag gcgagggccg cggccgtgat gcgggagatc
     6901 ctggtctacg gcgagcgcaa cctgcgcagg atcctggtgg gggaggccgg caccgccggc
     6961 gggccggctg gaggcggtgc ggtcggaagc ggctcggccg agggcggcgg cgccgcgggc
     7021 gggccggcgg gaagcggcgc ggccggaggc cgggcgtgag cctctcgaaa ctcaaggccg
     7081 gcgtcgcgaa gcgcgcgggg agcggggccg gtgccctggg ccgcctggac gtcgaggtcc
     7141 gcgaggaccg cggctcagtc gtcctcgagg gcagggtccc gacctgggag ctcaagatgg
     7201 agctcggctg gtacgccgcc ggcttcggct acaagggcgt cgtgaacgac gtcgaggtgc
     7261 ccgacgggaa gggcggcctc ctgggcgacg agcgcctgga ggcgccgccg ccggccgcgg
     7321 gcggggatag gcccctcgag ggggcggcat tcgacgccgt cgtcgtgggc ggcggcgtcg
     7381 tgggatgcgc gatcgcgcgc gagctcgcgc gttggcggat ctcggtcctc ctcctggaga
     7441 aggaggagga cgtcgcggtc cacgcctcga gccggaacga cggcatgatc cacgacggct
     7501 tcgcggccag gcccggcacg aagaaggccg cctacaacgt gcgcgggaac cgactctggg
     7561 agcccctggc gcgggagctc ggcatagcct tcgagaggcc cggatccctc atcgtctaca
     7621 ggagcgcggc ctcccgggcc gcctatcccc tgatggccgc ccgcgccgcg gagaacggcg
     7681 tggacggctg ggagtactgg tccaggaagc gcctcctcgc ggaggagccg aacgcggccg
     7741 acgaccagca cggcggcttc ttcctcccga gcgcgggcat cgtctcgccc taccgcgcca
     7801 cggtggccct ggcggagagc gccgtcctga acggcgtccg cgtcgccctg ggcgcctgcg
     7861 ccctcggctt cggcatggag gacggccgca tagcgagcgt gaggaccaac ctgggcgacg
     7921 tgagggcggg cgtcgtcgtg aacgccgccg gcggctgggc cgacgcggtc gcgggcttcg
     7981 cgggcgaccg cttcttcagc ctccaccagc gccggggcgt ggacatgatc ctggaccagg
     8041 gcgccggggc tttcctcaac cacatcatgt cgaggccggc cctggcggtc gccaaccgca
     8101 ggaccaaggg aggcggcctc gtgaagacca tcgagggcaa cgtcctcgtc gggccgaccg
     8161 cgtcggaggc cccgggccgc gagtcctacc cgacgacggt cgcggagctc cgcgagctcg
     8221 agaggcactt caagctcaac aagcggctct cgccctccat ggccatcacc tacttcgcgg
     8281 gggtccgcgc ctgcacctac gaggaggact tcgtggtgga gcgctccgcg cgcgtgcgga
     8341 acctcgtgca cgccgcgggc atccagtcgc caggcctcgc ctcggccccg gccatcgcgg
     8401 aggacgtggc ccgcctcgcg gtggaggccc tgtcggagac gaggagggtc gtcccgaatc
     8461 cctccttcga tcccgtccgc aaggcgagcc ccgagctcag gcgccttccc ctggaggagc
     8521 gggccgccct catcgcgcgc aatccctcct acggccgcat cgtctgccgc tgcgaggagg
     8581 tctccgaggg cgagatccga gacgcgctgc gctcgccgct ccccgtgggg agcctggact
     8641 cggtgaagcg ccgcgccagg ccgggcatgg gaaggtgcca cggcggcttc tgcacgcccc
     8701 gcgtgatgga gatcgtcgcc gacgagctcg gcatggacct ctgcgaggtc acgaaaaagg
     8761 ggccgggttc ccgtgtcgcg ttgggcgaaa ccaaggccgg ccgggaagga ggagcccgat
     8821 gatcgagagg gagaggcggg acgtggtcgt cctgggcggg gggccggccg gcctcgcggc
     8881 ggccgtcgcg gccgcggagg cgggggccga cgttctcgtc gtcgagcgcg aggagcggct
     8941 gggcggtatc ctcaagcagt gcgtccacga cggcttcggc ctcgtccgct tcggggagca
     9001 gctcgcgggg cccgagtacg cgaggcgcta cgtcgacctg gcggaggcca ggggcgtcga
     9061 gtcccgcctc gagaccttcg tccactcggt ccgccgcgag ggcccgggcg gcgccgcggg
     9121 cggcggcggc ccggccggcg caggccgcgc aacagaccaa ggaagcgccg ccggctccgg
     9181 cggcgccgcc ggcccgggcc ccctcgtcct gacctgcgtg agccaggacc ggggcgtctt
     9241 cgagatcgag gcgccctccc tcgtcctggc cacgggatgc cgcgagcgga cggacagaca
     9301 ggtcttcatc cacggggaga ggcccgccgg catcctgacc gcgggcctcg cccagtacct
     9361 cgtcaacgtc gagggcctcc tgccagggaa gcgggccgtc gtcctcggct ccggggacat
     9421 cggcctcatc atggcccgca ggctcaccct ggagggcatg gaggtcgagg gggtctacga
     9481 gctcaagggc gagccctctg gcctgtcgcg caacgtccgg cagtgcctgg aggacttcgg
     9541 catacccctc catctgtcga ccacggtcac cgaggtccac gggaagcggc gggtagaggc
     9601 ggtcacggtg gcctccgtcg acgcgcgggg ccaggcgatg gccggcaccg agcggcgggt
     9661 cgcctgcgac accctcgtcc tctccgtggg cctcatcccc gaggacgaga cggccctgtc
     9721 cctcggcctg gacctggacc cctcgaccag ggggccgcgc gtcgaccagg ccggcgcgag
     9781 ccgcgaggag ggcgtcttcg tctgcggcaa cgccctccac gtgaacgacc tcgcggacta
     9841 cgtctccgag tcgggcgcgg cggccggcag ggccgcggcc gagcgcgcgt tgggcggcgc
     9901 cgcgagccgc ccggagaggc ggctcgcgcc ggtgaaggcg ggccccggct tcctctacgc
     9961 ggtcccccag tacatagatc tttcgaggcc gggcccggcc gtcctatatt tccgctccaa
    10021 cgcgaccctg cgcgaggggg ccaggctcgt cgcgcgcgcc ctcggccccg gcggagagga
    10081 gacgggcctc ctcctcaaga agagctacgc cgccctccgc ccccccgaga tggagcggat
    10141 cgagatagac gtcgcctcca ttcccgaagg gacggcctcc gtcgccttcg agctcgagag
    10201 gaaggagtcg ctccgtggct gaacagagga gattcgtctg cgtggcctgc ccgaggggct
    10261 gcgccctcga ggtcgatgtc gcggaggcgc cggcgggggg ccgcgaggcc ctcgcggtca
    10321 ggggcaacgc ctgcaagcgg ggcgaggact acggccgcgc cgaggtggtc gatccccgcc
    10381 gaagcctcac gagcaccgtg aggaccgagg gcacgaggag gcggcgtctc cccgtcagga
    10441 cctcggggac cgtcccgctc gggcggctcg tcgaggccgc ccgatccctc gactccgtct
    10501 tggcgaggcc gcccgtgaag tgcggccagg tcctggctcg ggacctcctg ggcctcgggg
    10561 tggacctggt cgcgaccgac gacctcgagg aggagggcgc cgtgcccgcg gccgccgcca
    10621 agcccggcgc gggcgtagcg aagcccggcg cggccgcagc gaagcccggc gcggccgccg
    10681 tcaagcccgc cgcgggcgct cagccctcag ccggcgcgcc ccggcgcgga aggagctgac
    10741 atggaagagc tgctgctgtc gatcgacgcg ggcacccaga gcctcagggc cctcgtcttc
    10801 gaccgcgagg gaagactcct cgtcaagaag aagatcgagt tcgagccgta tgtccccggg
    10861 acgcccggcc gggccgagca ggatcccgag gtctactggc gggccctcca ggccgcctgc
    10921 cgcgacctca aggcgaggag gccggagctc atggccgcgg tcgcgggcgt cggcatcacc
    10981 tcccagcggg ccacgatggt ctgcctggac gaggcgggcg tccccttgag gcccgccatc
    11041 ctctggctcg acgtgcgcaa ggccaggccg ccctatcgtc ccggcctcgg gatgcgggcg
    11101 gccctcggcc tcgtgggcat gaacgaggcg gtcatgctga cccaggaaga gggcaagtgc
    11161 aactggatca tgcagaacga gcccgggacc tgggagaaga cccacaagta cgtcgaggtc
    11221 tcgggcttcc tcacgcacag gctcaccggc agcttcgccg actcgaccgc ctccctcatc
    11281 ggccacctcc ccttcgacta caagcggcaa gcctggtgca agccgggagc gctcaacgcg
    11341 aggctcttcc ccgtcccgag ggagaagctg cccgatcccg tggcgccggg cgaggtcgtc
    11401 gcccgcatca cccccgaggc ctcagccgcg accggcatcc ccgccgggat ccccctcgtg
    11461 gcagcgggca gcgacaaggg ctgcgagacc atcggcatgg gcgtcgtcga cccgaccaag
    11521 gcctcgctct ccttcggcac gacggcgacc atccagacga cgagcccccg ctacttcgag
    11581 cccctgcgct tcatgcccgc ctacccggcc cccatccccg gccgcttcaa ccccgaggtg
    11641 gagatcttcc gcggctactg gatgatcacc tggttcaagc accagttcgc ctacgaggag
    11701 gtccgcgagg ccgaggcccg cggcatcgtc cccgaggcga tgctcgacga gctcctccgg
    11761 aggacgccgg ccggcggcca cggcctggtg atgcagccct actggacccc gatgttcaag
    11821 atgccctccg cgaagggcgc catcatcggc ttcggcgacg tccacgaccg cgcccacgtc
    11881 taccgcgcca tcatcgaggg cctggcctac ggcctcaagg acggcctccg cgcgatggag
    11941 agggcgggcc gctcgaaggt cacggagctc gccgtctcgg gcggcgcgag ccaaagcgac
    12001 gagatctgcc gcatcacggc cgacgtcttc aacctgcccc tcttccgcgg ggcgacctac
    12061 gagacctccg gcctcggagc cgcgatggtc gtcgccgcgg gcctgggcat ccacccctcc
    12121 gtggaggcgg cggtcagggc catggcgagg cgggaccggg tcttcgcccc cgatcccgcg
    12181 cgggcggagc tctacgacaa gctctactcc agggtctaca agaagatgta cggcgccctg
    12241 gcgccgctct acgaagagat acgcgacatc acgaactacc ccgagcggct cggccgcgcg
    12301 gactagacga gaggagcgac cgatggccga gagagaattc agccccgcgt ggttcgaggg
    12361 cgagccgccc gccaagagct tccgctcgat cctcaagtgg ggggatccga gggccttcaa
    12421 gcacccgaac aggcgcctgt acaggctcat gaaggagacc ttcggcctgg acgacgagta
    12481 cttccgccat cccttcgaga cggggatgga gacggtgccg gaatccaggc cctgcgccct
    12541 cgaggcgcgc cacgtcgagg ccctgcgctc gatcgtcggg gcggagaacg tcttcaccga
    12601 cgtctacagg cgcctgggcg tcgcctacgg caagacgatg ctcgacgcca tgcggctccg
    12661 caagggaatc atcgagaaca tcccggacgc ggtcctccat ccgaggagcc gcgccgacct
    12721 ccgggccgtc gtcgactact gcgccgcgaa cgggatcccc gtctacgtct acggcggcgg
    12781 ctcctccgtc acccgcggcg tggaggccgt gaagggcggc gtgaccctgg acatgcgcgt
    12841 ccacctcaac aaggtcctgg ccttcgacga gaagaaccac acgatcaccg tggaggccgg
    12901 catgtcgggc cccgagctcg agcgccagct caggaacgcc gccgccctcc acggcgcgaa
    12961 gcgggcctac acctgcggcc acctgccgca gagcttcgag tactcggccg tcggcgggtg
    13021 ggtcacgacc cgcggctcgg gccagaactc gagctactac ggcaacgccg ccgacatggt
    13081 gatcgcccag gactacgtct gcccggcctg cgacatccgc acgggcgagt tccccgccgc
    13141 ggccaacggc cccgacgtcg acgagatcat gatgggctcg gaaggcgcct tcggcgtcct
    13201 ttactccgcg accctgcgct tccgcatgta catgcctgag aacacccgcc gctactccta
    13261 catcttcccg agctgggagg aggccaagga ggccgcgagg gagatcatgc agggcgagtt
    13321 cggccatccc tccgtcttcc gtttgtcgga tcccgaggag tcggacgtcg ccctcaagct
    13381 ctacggcgtc gagggcacgg tcctggacac gctgatgcgg ctccgcggct tcaggaaggg
    13441 cgagcgctgc ctcatgctcg gcacggccga cggcgaggcg ggcttctccc gccacgtcgc
    13501 ccgcatggtg gggcgcgtcg cccgccgcca cggcgcgatg tccaccacgg gcctcgtcca
    13561 gcgccattgg gaggaggggc gcttccgcga cccctacatg cgcgaggacc tccaggacta
    13621 cggcatcatg atggacaccc tggagtgctc ggtgaactgg tcgaacctgg agaccgtgca
    13681 cacgggcgtg cgcgagttct gccactcgag gccggacacg atctgcatga cccacatgtc
    13741 gcacttctac ccgcagggct gcaacctgta cttcatcttc atcgcgaaga tcgaccggat
    13801 cgacgagtac ctggcctacc agtcgggcat cctcgacagc atccagaagc acggggccac
    13861 gatgagccac caccacggca tcggcaaggc cttcgcgccc tggctcgagg gcgacgtcgg
    13921 ccccgaggcc atctccctct tccgcgccat caagcggcac ctggacccga agggcatcat
    13981 gaatcccggc ggcaccatcg ccctcgacct gccggacgag aagcggcgga tactcaggaa
    14041 gccggggcgc taaagagggg ctccgcgcga ggcggcgccg cggctgagtc tcgccgggcg
    14101 ccggccataa ggcccctccc gggcggccgc ggcgcgatcg cgaccggtca gcgtgtcgtc
    14161 gtgcgcgccg gacgggccgg gcgacgcgcg gccgcctagg ctcccgtgaa ctcgaggaag
    14221 cggccgggcg aattttccgg ttcgggatag gctccccgct gtcggggcgg gaggaggagg
    14281 gcgaggcggt tcatgagctc ttcagtcatg tcgagcctgt cccgcctggt cgcgcctcct
    14341 tgcggctcct tgagcctgaa gctctctccg acgcggaagg ccaccctcgt ccgccggagg
    14401 cgcctcaggt tgggccagaa gcgctcgcag cccgtgatgg ccacggggag gacgggcgcg
    14461 ccgctccgga gcgcgatctg cgccacgccg ccgtgcccgc gctggaggcg gccgtggccg
    14521 ctcctcgtgc cttcgggggc cagggccacg acgcggcccg cggccagcgc ggccatgatg
    14581 cggcgcatcg cgcccaggtc ggctccctcc ctgtccaggg gaatcgtctc ccaggcgtcg
    14641 gccaggaatc cgaggagggg attgtcccag gtctcccgct tcgcgacgcc gacgaggcgg
    14701 cgggaggcga ggtagctgta gaggaggggg ccgtccagga agttcacgtg gttgatgacg
    14761 acgatgaggg ggcccttggc cgggaccttc gcgagctcct tcgagtcgac cctgcacagg
    14821 agtccgagga ggctccggat gaggggagtg aggacgcgcg aggccggcct cactcgtcgt
    14881 cctcttccgc ggcggcgacc gcggtccgag agccgccgcc cggggcgcct tcggccgccg
    14941 cgacggccgc gaggatcgcg gcgccgtacc gccccgcctt gacttcgccc atgccgaaga
    15001 cctcgaggag cccgggcagg ctcgacggcc ggcgcgagac gaggtcgtag agggtccggt
    15061 cggcgaagac gacgtagggc ggcaccccgg cctcctcggc caggcgcctg cggagggctc
    15121 ggagggaggc ggcgagggca tctgctcccg gccccgaggc cagcgccgag gcgaggccgg
    15181 ctcccgccgc tcccgcggcc gcgcgtgcgc ccgaaggcgc gtccttcctg ccgcggggcg
    15241 gcaccgttcc caggatgggc gatcggtcct tgaaggcggc gtaggcgagg ggcgtcaggc
    15301 gcagtacgcc gaactcctcg tcgcggtcca ggtagccctt gcgcgcgagc tggcgcgaga
    15361 ggtccatcca ctgcgccctc gtccattcct tgccgatgcc gtaggtggag agctccgcgt
    15421 ggccgaggcc caggacccgc tcgttccgcg agcccacgag gacgtcggcc acatggccgg
    15481 cgccgaagcg ctcgcccgtg cgcttcacgc aggagaggaa cttgtaggcc tcagtcgtca
    15541 cctcggtctc cggagcggcg ccggcgccgg ccgcgccggg gacgcaggcg tcgcaggctc
    15601 cgcagcccgc ggcggcgtcc gggcgcgcga tcggcggtcc cgcgtaggcc tcgccgaagt
    15661 gggagaggag ggctaggcgc cggcagccgg gcttctcggc gtagcggagc atcgcggaga
    15721 gggccgcctc ggccgccagg gcctcgtcgc cctccttgcc cgaaaggagg gcccgcatct
    15781 tctgggcgtc gccgtagccg tagaggagga ggcacctcgc gggtagcccg tcgcggcccg
    15841 cccggccgat ctcctggtag tactgctcca ggctcttcgg gaggtcggcg tggacgacga
    15901 agcgcacgtc gggcttgtcg atgcccatgc cgaaggccgt cgtcgcggcg acgacccgca
    15961 cctcgtcgtc gatgaaggcg tcctggttcc gcgcgcgctc ctcgtcggga aggccggcgt
    16021 ggtagggaag ggcgggaatg ccccgctcgg agagccgccg cgcgacctcc tcggccctgg
    16081 cccgcgagaa gcagtacacg atgcccgatt cctccggaaa gcccgcggcg aaggaggcga
    16141 tctcgtcgta gccgccgttc gggccgcggg gcctcgcctc caggaagagc tcgggcctgt
    16201 cgaagctcgc gacgatctcc agggggtctc ggagcgcgag ggccctcgcg atgtcggccc
    16261 tgacgcgggg cgtcgcggtg gcggtcaggg ccaggaaggg cgcgccgggg aaggcctccc
    16321 ggagggagcc gaggaggcgg tactcggggc ggaagtcatg gccccactcc gagatgcagt
    16381 gggcctcgtc gacggcgacg agcttgacgc gcagggagga gaggagctcg ctcccgcggg
    16441 gcgagaggat ggtctcgggc gccgcgtaga ggagccgcgc cccgccagcc cgcagggagg
    16501 cggcgccggc ccgccactcc tcgggcgaga ggctcgagtt gaggaagatc gccggggctc
    16561 cgagctcgcg gaggaaggcc acctggtcgc gcatgaggga gatgaggggg gacacgacga
    16621 gggcgaggcc ctcggaggcg agggcgggga tctggtagca gagggacttg ccgccgcccg
    16681 tgggcatcac cgcgaggacg tcgcggcccg cgagcgcggc ctcgatgatc cgctcctggt
    16741 tggggcggaa gttgtcgtag ccgaagacgc gcttcaggag ctcgcggggg gtctcgctca
    16801 tgggcctgag tatagaccgg gaaggccgcc cgggccaacg ggccggggcg ccggcctgcc
    16861 cggcacgccc ggcggtcgcg cgcggaggcg cggccccgcg cggccccgcg cggccccggc
    16921 cgcgaccgcc ggcgcgggct ccgcgggacg catggcggcg ccggccccga ggggcgcggg
    16981 acggccacga tccgccggcg gggctgtcgc gccgcgggtt ccgggctata cttcaaggcg
    17041 acgcgaaaag cccatatccg actcaaggag tagtgatgct cgaattcata gtcaagggag
    17101 gcctcgtcct ctggatcatc atgaccctgt ccgccgtggg agtcgccatc atcgtcgagc
    17161 gcctcgtcta tttccgcaag atccgcgtgg acgaggagaa gctcttcctg cgcatcaagg
    17221 cctcgctcga gaagggccac ttcgacgagg ccctcgcgat ctgcgaccag aacgtctcgc
    17281 cgctctccgg cctcatgcgc gtcgggatcg agcataggga gtaccccgag accgtccaga
    17341 aggaggtcct caaggacgcg gccgcgcagg aggtcccgcg cctggagaag aacgtctccg
    17401 cgctgggcac catagcgcat atcgcgcccc tcctgggcct cctcgggacg gtgacgggca
    17461 cgatgaaggc cttcggcgtc ctggggcgct tcggctccgt cgccgacccc tcggtcctcg
    17521 cgcgcggcgt ctccgaggcc ctcatcacca ccgtcggcgg catcgtcgtc gcggtgcccg
    17581 cggtcatctt ctacaactac ctggtcacga aggtgaacct catcctcgtg cagatggaga
    17641 accaggtgaa cgccctcgtc ctcatgatca acgcgtccag gctcaaggcc gcgtcccggg
    17701 agggagggaa ggcatgaggc tcgagcgcag gctgaagccg acgatcaaca tcgacatgac
    17761 gcccctcatc gacgtcgtct accagctcgt catcttcttc atgatcacga gcgtcttcaa
    17821 gatggccccc ggcatcgcgc tggagcttcc cggcgcggcc accgcgagct cggtcgcggt
    17881 gaccgagctc aggatcgtcg ccgtctccga ggacgagatc tacgtcggca aggagaggac
    17941 gagcctcgcg ggcctcgaga ccctcgtgcg gcgcgcggtc gagggcaccg aaccgaagga
    18001 caggcgggcc gtcctggagg gcgacaaggc catgccctac cagctcatgg tgtcgatcct
    18061 ggacgcgctc agggagaacg gcgtcgacgg ggtcggcctc cagaccagtc cgggcggcgg
    18121 ggcgggggcg aaggcggcgc ccgcgaaggc ctcgccgtga ggcgggagga ctggctcagg
    18181 gcccaggaca ggaagcgccg ggtcctcgcc tgggccgcca ccgccgcggc ctatctcctc
    18241 gcgctcggcc ttgtcctcct cgtcgagggc ttccgcgtcg aggaggtctc cgactacgcg
    18301 ggacccgtgc gcgtgagcct cgggagcccc gagggcaacg ccgcgcgcgg ccttctcgag
    18361 tccccgcccg agctgtctga gccgccggcg cccgcgcccg ccgagccgac gcctccggaa
    18421 cccgccgccg cggcgccgac ctctcccgcg acccccgcgc ccgccgcccc ggcgcagccc
    18481 aaggcgcctg cggccgcgaa gcccgcgccc gcggcggcca agccggcccc ggcggccacc
    18541 gcatccgcgg ccgcggcccc gtcggccccc gccgctccga cttcgccgcc tccgcccgag
    18601 gtcgcgcgcg gcacggaggc tggcaacgac tacgagacga gcttcggcgg cgactcgggc
    18661 ttgattagac ggagcttgaa ggtcccgata tatcgatact taccgccacc gttttatctt
    18721 gatcagaaat atgttgattc gatcgctggc ctgcctgacg cagtaaatcc aatgccagaa
    18781 gaagcgaggc ggcgactctt gatcacgtat tatgcgaaat caggagatgt atggaaacaa
    18841 aaactcccag tccctctcga tgtacgagag agactttggg tagttctcga ggaggcgggc
    18901 tttgatcctc ggcgagctga ctataaactc aacaatccca aacctatcac actctctttc
    18961 acgatggtgc caatcaaaaa ccagatgcct gaactacgcg atctccgcat catcgagtct
    19021 tcgggttttc ctgatctaga cgaagcagtc atgtatggtt ttcagaaggc tggtttctct
    19081 tcggacgcaa caaaagccgt cacgggccgc ttcgtctacc gcttcaacta gggacgagga
    19141 cgcgggccca ggtcgggtgg agagtcagtc ggaccttgcc gttccgcacg gggaagcgct
    19201 cccctccgtc caaggcgtcg cggaggaagg ccgcggggaa gggcaccgga acctcgagct
    19261 ccaccgcgcg cttctccgcg ttgagcgcca cgacggccgg gccggcctcg caggagcgct
    19321 cgaaggcgaa ctggcgcggc gcgacgagga gctcgcggta gccgccgcgc ctcaaggccg
    19381 ggacggcctt cctgagcgag gcgagccgcc ggacggcggc ctcgagcccg gggacctccg
    19441 gccccgcctc gagcgcctcg acggaaggcc gcagcggcgc gtccgagccg cccgcctttc
    19501 gccccgccgc cccgcgctcg gagccgtagt agagcgaggg cgggcccggc atcgtgaaga
    19561 ggaggcagta gagggggaag aggagggcct cgtcgcccag gagggaggcc gcccggtcca
    19621 cgtcgtggtt gtccgcgaag gcgtagagat gaaggccgcg gtagatcccg ccctcgccga
    19681 actgccgctt gagggcgtag gcgatctcga agtaattccc gtccttgagg ctcgaccaga
    19741 ggcccttcca gcattcgtag ttcgtcgtcg agtcgaggag gcccggcgcg gcccagcgac
    19801 ggtagtcgcc gtggaccacc tcccccatga gccagaagtc cgggcgccgc gcccggcagg
    19861 cggcggcgag ctcccgctgg aactcgaggt cgaccaagtc ggccgcgtcg agcctgaggc
    19921 cgtcgacgcc gtagtcgtcg atccacgaga ggctcgccgc cacgagctcg gcccggacct
    19981 cgggattgcc ggtatcgagg cgcgcgaggt cgaggcagcc gttccagccc tcgtaggaga
    20041 agggatcgcc cagggggctc ggccgcgaga agtcgaggcc cctgaaccag gccgcgaagc
    20101 gcgaggagcg gccgcgctcc gccacgtcgc ggaaccaggg gtgcccgcgg cccacgtggt
    20161 tgaagaccgc gtcgaggacg aggcgcatcc cgcgccggtg tagcccgtcc gagaggcggg
    20221 cgagcgtcga cgagtccccc aggcgccggt cgaccaggcg gaagtcgacc gtgtcgtagc
    20281 cgtgcgcgct cgactcgaag acgggtccca ggtagagggc gtcggctccc agctccgcga
    20341 cgcgcccgag gcgctccgtg aggcgctcga ggcgcgggat cggcgcggag gagaagtcgt
    20401 tcctcccggg cgcgccgcag tagcccaggg gatagacatg atagaagacc gagtcttcgg
    20461 cccagtgcgc catgatgcct ccatggaatg tcatttacta cctaccggta ggtatattct
    20521 gaacgagcac agtgacggcg agcgggcggt cctgccgcgg gcggtcctac cgcgcaggag
    20581 aagccgcgcc gcgcaggcga agcctggcgg ctcgggctgg gcccggccgc cggccgcgcc
    20641 ttgctaggag aagatcccgt acatgaactg gcggacagcc tcacggatcg tccgctcgtc
    20701 caggccgctc ctccccgcga gatggaagcc cgcgtagctg tcgatggtgc cgatgaaggt
    20761 cccggcgaag gccctggaac ggccgcgcat gttcccgtgg tccttctccg ccgcggcgaa
    20821 gaaggagcgc acggcctcga agagggcctc gttgagcgac gcggccgcct cgcccgcctc
    20881 gctgcccggg ggggcgaagc tcatcgcgag gcgcatgcgc gcgaagacgg gatcggcgag
    20941 ggcgaagcgg gagaaggcga gggcgatctc ctcgagcccc ttggggacgt cgcgcccgta
    21001 gcgcgcggcc gcgcggaccg cggcgaggag ggggccgccc cgctcctcga tcgccgcgtc
    21061 caggagaccc cgcttggagc cgaagtagtg gtacagggtc ggcttggtca cgccggaggc
    21121 ttccgctatc tcggcgaccc cgacggcctc gtagccgcgc tccgagaaga gcctcgcggc
    21181 ctcggccagg atgagctcgc ggttcccgcc cgtttcttcc atgcggccac tataccgaac
    21241 ggtatagcgc tggtcaaggc ccgccgcgcc aagcccctcg gttcaacgcc gcgccgcgcg
    21301 actccgcgcg tcggcaagcg gcgcggcgca gcgcgactcc gcgctatatg gcgccgcgcc
    21361 cggccgccta ggccttcctg ccgacgaaga agagggaggg ggcggagcgg aaaccgaccg
    21421 agcgggcgcg ggccaggagc tcaaggcccc gggccttcat cgccgcgcgg gcctcggggg
    21481 ggacgcgggc gccgccagcg ccttcgccca gggcctcgat cttgcccgag taggtctcga
    21541 ctatcttctc gatctcggcg aggagctcgg ggtcgagggg atcgccctcc tcctccacga
    21601 tctcgtccca gctggactcc gcgaagcccc cggctccggc ggaggcctcg acgaggccgc
    21661 gcagggccgc cctgccgtag gtctcagcgt ggtggacgcc gcccgcgctg tcgacctcgg
    21721 cccaccagtg gtgcatgagg acgtgggtca tggccgggcc ttcgcctgag tcgcggtgca
    21781 tctccaggac gacgaggcgg ccgccggggg cgaggacgcg aagggcctcg gagaggacgc
    21841 gggccggatc ggggaagtgg tggagggagt tggagaggcc gacgaggccg aagctcgcgt
    21901 cggcgtaggg gagggcggcc gcgtcggcct cttccaccct ggcggcccgc gtggcggcca
    21961 ggttcttccg cgcggcctcc aaggcacggg gggccgagtc gacggcgacg atctcctcgt
    22021 aggaggcgag gctccccgcg agcctggccg cgaaggcccc cgagcccgtc gccaggtcga
    22081 ggaccctgcc cccgtcgatg tccttgagcc tcgcgtcgat gatgtctatg gcttgcctcc
    22141 cgccgcggct tgcggcgcgg cgaggacagg atagccgcgc gaggtccggc gcgccatccc
    22201 ccgaacgagg ggccgggaga cggcggccag gacttgaggc gggcgtctcg gccgcggacg
    22261 gcgcggtcgc gcagcgccag ggcgccgacc cggccgcgga cgggcttccc cgcggtcgcc
    22321 cggaggcgcc cgcccggccg cggattcacc cgaatagggg agagtctcag gccgcggctt
    22381 tccgctagag tggcgcccgg acgggaaggt cgcctctcga ggctcgaatc ccggccgaaa
    22441 ggatgcgtcg gtatgtcgtc gctcggcatg tgcgtcgagc tccgggccgt agaggcgcgg
    22501 agcgatctcc tcggtccgtc ctatcccgcg gcctctccgc gcggggaaga cgccgcccgc
    22561 cggacgcgcc ggcgcggttc cggactcaag ggcctggccc tgctcctctt caggcgctga
    22621 agaacccggc ccctggccgg ccgtctcgcg gaagcttccg cctggcgcct gcctgacggg
    22681 caagccctcc gcccgacctt tgcctccaag gcgggcgcgc gcccatgaag gccggccggc
    22741 ccgatagtcg tgtcggccaa cttcccgcgt ctccccgagc tcctcaaagc cggcttcccg
    22801 tgctaccatg tcggcttgcg cgcctctccc cgcgaggagg ggctgaagga gcgacacatg
    22861 ggatccctgg ccggaagctg cccagaagcc tacgccgaat tcgggccgcc gcgcctccgc
    22921 gcgggcctgg tcaggcgcga gaggctcttc ggactgctcg atctgggggc cggccgccgc
    22981 ctcgtcgtcg tcaagtcgcc ggggggcttc gggaagacga cccttctcgc ggactggctc
    23041 caggccaggc gccgccgcta ctcctggctc ggcgtcgagg aggggccgct ggaggggcgg
    23101 gagctcctcg cgctggtcgc cgcctccctg cggagctccc tcggcgaggc ggggaaggcc
    23161 ctgcccgagg agggggaggg cgcgggcctc gtccgcgcga tagccgccgc cgtcggggcc
    23221 ctggacgagg ccttcagcct ggtcatcgac gactgccacc tcatcggcga cgggcgggcg
    23281 ggcctcctcc tcctccgcct cgcggaggcg ctgccgccca acgcctccct cgtcctttcg
    23341 ggcaggggcg agccttcctt ccccctggcc aggctccggt cccggggcga gctcctcgag
    23401 atcggcgccg ccgagctccg cttcaccgcc gccgagaccc gcgacttcct ccgcggcccc
    23461 ctcggggact cctactcgga cgacgaggcc gccctcctgg gcgcgaagac ggagggctgg
    23521 gtggccgccc tccagatggc cgtcctgggc ctgcgcggcc gggccgaccg gcgggccttc
    23581 ctcgagtcct tcggcggcgc gcaccgcgac gtcttcgact acttcgcgga ggagaccttc
    23641 gcggccctcg atcccggact cctggccttc ctcctgccct gctccgtcct cgagaccctc
    23701 tccgctccgc tgtgccggga gctcacgggc agggaggact ccgccgccct ggtcgagcgc
    23761 ctcgtggacg aggggctctt cctcctcccc ctggacgacg agcggcgccg ctacgccttc
    23821 cacggcctgt tccgggaggc cctcctccgc cgcctggagt cggacctgcc ggaagccctg
    23881 ccgaagctca ggctggcggc cgccggctgg ctggccgcga acggccagcc gctcgaggcc
    23941 atgcgcctgc gcctcctcgc gggcgacgcg gaaggggcgg ctgccctggc ggaggggcgg
    24001 gccctctcgg ccctggagag cggccgggtc gaggacctgc gctccgccat agccgtcctt
    24061 ccccgggagg ccgtcgagtc gcggccctgg ctcgccgtcg ccgccgcctg ggcggccgtc
    24121 tacgcggggc gcctttcgga cgcggccgcc ttcgccgacg cggcggaggt ccgcgcctcc
    24181 gcccagtccc ggatcatcgg ccatgtggag gccatcaggg cctaccgggc caacctggaa
    24241 ggggacggcg aggaggcgaa gctccgcgcc ctccgcgcgt tcgagcttct gcccgcgagc
    24301 gacagcatgg cccgggccca cgccgggaag agcctcggct tcggcttcac cctcctccac
    24361 gactacgccg cggcctcccg ggccctcgag ggggcgatcg aggaggcggg ctcccgcggc
    24421 ggccaccaca tccgggtcct cgcggcgaac gacctggcct acctcgccat gacgcagggc
    24481 cgcttctccg aggccgagga gatctgccgg cgcgagatcg cggcgggctc gggcggcggc
    24541 cgcgaggctc ccgtcctcgg ctgcctgcat agcctgcttt cgaacatctg cctctcgcgc
    24601 ggagagatcc ccgccgccct cgagcaggcg aggctaggcc tggagatggg ccttgcctgg
    24661 ggcaacatgg accgcctcgt cttctcctac gtcatgtacg cgacgaacct cgcggcggcg
    24721 ggggacaggc ccgccgcgag ggcggccctg gagcggctca gggccctccc cgacctcacg
    24781 ccgaggcacg cggcggtgat cctcatgacc gcggcggaga tcgatcccgc cggccccgac
    24841 gcggcgcgca tggagcgcat ggccccgctc aaggacgaga acttcaacct ctccttccag
    24901 gcggtccgcg cccgcgccct cctcgcgaag cgccgggcgg ccgagtgcct ggccgtcgtc
    24961 ctgccctccc tggagctcgc ccggcgctgg aacctcaggc ccgtccagct cgacgccctg
    25021 gtcaacgccg ccctggccca gcggagcctg ggacgccggg aggaggcccg cgccgccgcc
    25081 gccgaggcca gggccctggc ggaggagctg ggcgacctcc tgcgcatccc cgcccgcggc
    25141 cccgccatcc gctcccttct ggaggaggag ggccttctct ccggcagctt cggcgcccgc
    25201 ctcgccgcgg ccttcgacct cttcgtggaa aggcccgcgg gggaggccga cttccgcgcc
    25261 ggcgagccgc tctcgccccg ggagcgggag atcctctccc tcctcgtcca gggccgcacg
    25321 gcggaggaga tagcctccct cctcttcgtc gccccgagca cagtgcgctc ccatgtgaaa
    25381 agcctctacg ccaagctcgg cgtccatcgc cgcatcgagg cggccaacaa ggcgcgcgag
    25441 ctcggaattc tttagggtcc ggcctcccgg gggcctccct gggtctcagg ggttcaggaa
    25501 tgcttcccga aatgcgggaa aaccacccat gattccccat ctttgaggat gccgcccgcc
    25561 cttgaccgac tccacactcc tccttcggac gggaaggccc cttcgggcgc cgcccggtcc
    25621 gcaatccggc atgtggagga acgaaagcat gaaacgattc gcggtatccg tcctcgtcgt
    25681 cgcgctcgcg gcggcggggc tgtccgccca ggccaagggt ccgatcgtcg acaagatcct
    25741 gctcgacgcg aagacccagg aggacatcgc cctcaaggac gtcgcctccg gcaggagcga
    25801 cctcttcacc tacgacgtca gcgcggcgac cttcaaggcc ctgcccgacg acatcaggaa
    25861 caggctcgac ccctacgcca tcaccggcgc ctcctacaac accctcttca cgaacccctt
    25921 cccgaacaag gccccctacc aggccaagcg cgacggcaag gtcaccctga acccgctcgc
    25981 catccgcgag gtccgcttcg ccctcaacta cctcatcagc cggaagcaga tcatcgacga
    26041 gatcctctcc ggcggcggcc tcccgatgta cacgcccgtc atccccggcc agcccaactc
    26101 ctcccgctac ggcctcgtcg cctccaagct cggcttcacc gccggcggca acgagaagaa
    26161 ggcgatcgcc gacataggct cggcgctgga gaaggccgcg gccctcccgg agaacaaggg
    26221 caggctcgtc cggggcgacc gctactggac cttcgacggc gagcccctcc tcgtcaagtt
    26281 cctcatccgc gtcgacgacc cctccgtccg cctcccgatg ggccgctaca tcgcctccca
    26341 gatcgagaag gccggcatca aggtcgagcg cctggagtgg gaccgcacga agtgccgcga
    26401 gacctacgac cagaacgatc ccgccgacct gggctggaac ctctacacag agggctgggg
    26461 cggcggccag accctggcct actgggacca gagcatcgcc cagatgtacg ccccctggat
    26521 gggcgacatg cccggcggca acagggcggg cgcctggagc tacgagcacc ccgagctcga
    26581 caagctgacc tccgactcgg tcaacggccg cgtgaaggac gaggcggact actacgccaa
    26641 gtgcctcaag gccaccgagc tcggcctcaa ggaagcggtg cgcctctacg tcgtagcgac
    26701 gacgaactac acctgcgcca gcaaggaccg cttcaacaac cgcatggtct acggcctggg
    26761 cgacggcctc aacaacttct cctactacac ggccgacgtg aagccggaca aggacggcca
    26821 gaaggtcttg aggatctccg gcttctcctc ccgcggcgac ctcttcaact ccgcctggga
    26881 ccccatcggc cccgacggct tctccgacac ctactccggc acggtcgcga ggcagctctc
    26941 cgacaacgag tacaacgcga accccgtcac cggcatcaac atcccgatga tggcctcctg
    27001 gtccaaggtg aagaccgaca tcgcggtgtc cgccgacggc tccctccagg gcaggctcgc
    27061 gatccccgcc gaggccgtct tctacaacgc cctgacccag aagtgggact cctccgtcgt
    27121 ctacgtcgac aggaagggcg acggctccga gtacggctac gcgaagcgct ccgagaatcc
    27181 cgccttctcc aaggccttct ccaccgcgac cttcgccttc aagcccggca agtggcacca
    27241 cggccgcgcg atgggcatcg tcgactaccg ctacgcgatc gcccgccagg tcgagctctg
    27301 cgtccagcgc ggcgcggacg acaaggtcta cgaggagagc tacgccgcct cctggaaccc
    27361 gaacctcgtc aggttcaagg gctatgtctt caacaaggac ggcaccctca ccgtctacgg
    27421 cgacgcgaac tacccgatgg accaggacca gctcgccacc ctgctctgcc cccagctcat
    27481 gatcgaggcc tccaactacg gcgacgtcct cccctgggag atccacgagg gcctcaagta
    27541 cctcgtcgcc gagggctccg cctccaagac cctctaccgc ttcaactccg actccaactt
    27601 caccgaggtc gacctcatca gccagcgctg cgtggccgac ctcaaggcca agctccagga
    27661 gctcaaggcc cgcaagcacg tccccgtctc catcgccgcc tgggtgacgc ccgaggaagc
    27721 cgccaaggcc tacgaccagg ccatcgcctt catcgacaag cacggccacg ccatgatgtc
    27781 gaacggcggc ttcatcctgg accgctacga cgccgcgaac aagaccgcga tcctcgtcgc
    27841 gaaccgcgat cccgcctatc ccttcgagaa gggccatttc gccaaggccc tcgcctcctc
    27901 ctacgcccgc atcgagtcgg tcaaggtccc ggcctacaag aagggcgccg acctcgtggt
    27961 ctccgccacg gtgagcgagg tcgccttccc ctccaacggc gcgaagcccg ccgccaagct
    28021 cacggccaag gtgaccctcg tcgcggacaa ggactacgtc ttcgccgcca agctcgccaa
    28081 ggccggcacg atcgaggccg cgatccccgc gaaggtcctg gggacgctca aggccggcag
    28141 ctacaccctc gtcgtcgagg cggccctcgg caacgaggcg ccgacggtcg agaccgccaa
    28201 cgtcctcgtc ttctaaaagc atcaaggact cgagcgccct cccccgcctc cggcggggga
    28261 gggcctcctt gaaggccgaa ggcgcgagcc ttccgccttc ggggaggaaa atccagcacg
    28321 caggacaagc catgtacgga aaatacgtcc taaagcgggc gggctacggg atcctgatgt
    28381 acgtcatcat gatcttcgta tactcgacgc tcttcaacag cgtcgccgac cgcacgatga
    28441 gctccctcat cgacgagcag gtcgcgcagg agatgacccg ctacaagagc ctggacgagg
    28501 cccagcgggc cggcctccgc gaggagcgca tccaggcgaa gcgccgccag taccacctgg
    28561 acgagcccct cgcgagccgg atcttctggc gcggcgtcga cacgatcacc ttcaacttcc
    28621 gcaactcgac catcatcaag gcctccgacg gggaccgccg cgtcctcaag atcgtcggcg
    28681 aggccctgcc caggaccatc gtcctcttca cgacggaggc ggccctcgtc accgtcgtcg
    28741 gcgtcatgat ggggctcggc ctcgcccgga agccgaacgg ccccctggac cgcttcaact
    28801 cggggatcac gatgatcacg aacggcctgc cggcctggtg gctcggcatg ctcatgatca
    28861 tggtcttctc ctacgccctg ccggtcttcc cctcgggagg gatccgctcc aacccgccgc
    28921 cagagggcct catgggcgtc ctcgactacc tctggcacct cgcgctgccc ctcgtcaccc
    28981 tcaccctcct gggcctctgg agcaccgcct atatcacccg caacatcgtc ctctcgaacc
    29041 tgcaggagga ctacatcatg gcggcccgcg cccgcggcgt gcccgagcgc ctcgtcctct
    29101 tcagccacac gctcaggagc tcgatgcccg ccatcatgac cctcgcgatc ctgggcctct
    29161 tcggctcgat cgcgggcaac atcatcgtcg aaggcatctt cggctggccc ggcatcggca
    29221 acctctactt cgtcgccgtc cagcagaacg acgtacccgt cctcatggcc accctcgcga
    29281 tcgagaccct catcaacatg ggcggcttcg tgctgctcga catcgtctac gggctcctgg
    29341 atccgcgcat caagactgga ggcagggcat gaacctccgc gacagcctgg aaggcctcgg
    29401 agccttctgg aaggaattca gcaagaacaa gacgggcctc gtcgggctcg gcctgctcgc
    29461 cgtcttcgtc ctcctcggcc tcttcgggac cctcgtcgtc cccttccccg gcgccatcga
    29521 ccactggcgg gacatcggct tctggcagga caatccccag gccgccccgc ccgtctggac
    29581 gaactggttc gcgaagagga agggcgtcgt gtcctccgtc ctcgaccgcg cccaggcgga
    29641 ggagtcgacg ctcgactcgg gcgcgcgcgt ctcctccctc accttcgact acgacttcgg
    29701 ctacgacctg cccccgcgcg acgtcgtcgt ccgcttcgag ggctcgggcc tcgtccccgt
    29761 gaccatcacc ctggagcgtc ccgacggcct ggtcgccgag ctctaccgcg cccagctcga
    29821 gctctccgag ggcgacgtcc agcgggtctc cctggcccgc aactgctccc aggaggtcgt
    29881 cgagttcctc cgcgcgagcg acgaggctct cgcggcctcc ctggacgccg acctcgtgaa
    29941 gccggtgagc ctcctcttct cgcgcctctc ccccggcatg gcggagagcc cggaggccct
    30001 caagggcgcc tacaggctca aggtcaacgc cttcctcttc tccgaggacg cccgactcgc
    30061 gaaggcctcc gcggtcgtgt cgggccacgt ctccgggatc ttcggcacgg acatggccaa
    30121 gcgcgacgtc ttcaccggcc tcgtgatcgg catccgctgg gccctcgtca tcggcctcct
    30181 cacctccatc gtcacggtga tggccggcgt gctcctgggc gtgaccgccg cctacatggg
    30241 cggcgcgacc gactggctcc tctcccggct ctacgagctc atcttcctca tgccggtcct
    30301 gcccttcctc atcgtcgtct ccgccatctt caagccgacg atctggaccc tcatcctcat
    30361 catctgcctc ttcttctggg ccggcccctt caagccggtc tactcgatgg cgctgcagat
    30421 caaggaggag accttcgtcg aggcctcccg cgccctgggc tcgcggagct ggcgcatcat
    30481 cctcaggcac atcatcccga tcctccttcc ctacagcttc gcctccatgg cgctcgcgat
    30541 cccgagcgtc atcgtctacg aggcgagcgt gagcctcctc ggcctgggcg actcctccat
    30601 cgtcacctgg ggccagctcc tcaacgacgc cctgggccag ggcgccgtca tcaaccagct
    30661 ctggtggtgg gtcgtgccgc ccgggctcat gatcgccctc atgggcatgt ccttcgcctt
    30721 cctcgggacg gcgctggaca agatcctcca tcccaagctg aagaccaggt gaccccatga
    30781 gcgacaacaa ggacaagaac gcgaaggagc ccgtgctcga ggtcaaggac ctcaggctcc
    30841 actatttcac ctcgaagggc gtcgtgaagg cgctcgacgg cgtgagcttc cgcctggatc
    30901 ccggcgaggc cctgggcctc gtcggcgagt cgggctgcgg caagacgacg acgggcgtgg
    30961 ccctcctcaa catgccctcg cccccggggc gcatcgtctc cggctccatc aaggtgggcg
    31021 gcgtcgagat cgtcgggatg gaggagcagg agctccgcag gaccgtgcgc tggtccagga
    31081 tctccatggt cttccagggc gcgatgaact gcctcacccc ggtgtacacg atccgcgacc
    31141 tcatgctcga gacctggcgc gtccacgacg acgccgagcg ccccgacctc cccaaggtgg
    31201 aggccctcat gcggaagtac ctggccctcg tgggcctgcc cgagcacgtc ctggggcgct
    31261 acccccacga gctctccggc ggcatgaagc agcgcgtcgt catcgcgatg gccctcttcc
    31321 tggagcccca ggtcatcgtg atggacgagc cgacgacggc cctggacgtc atcgtccagg
    31381 cgcagatcct caacctcatc aaggacctga aggccgagcg gggcatctct tacatcttca
    31441 tcacccacga cctcgcggtg gaggcggagg tggcggaccg gatcatggtg atgtacgccg
    31501 gcaaggccgc ggagatcggc acgaacgagc agatcttcgg cgaggccggg ccggcccatc
    31561 cctacacgag gaagctcctc gcctccacgc cgcggatcaa gtcgcgcgtg agcgagctcg
    31621 ccttcatacc gggctccccg cccgacctcg tcgacccgcc gccgggctgc cgcttcgcgc
    31681 ccaggtgcga gttcgcgaag cccaagtgcc atgaggcgga gccgcccttc ggcgagatcg
    31741 agagcgggca cttcgcggcc tgctggaaag cgatggagga tcccgaatat gagcgcaatt
    31801 gagaccggcg cggcccccgc cgccgcggcg cccgccgccg acgcctcggc cgtcctcgag
    31861 cttcaagacc tgcggaggga gttccgcgtc caccggggca agtccgtcgt gcgcgccgtc
    31921 gacggcgtga gcctcaaggt cgcccggggc gagatactcg gcctcgtcgg cgagtccggc
    31981 tgcgggaaga ccacgatcgg caagctcgtg atgaagctca tcgcgccgac gggcggcagg
    32041 atcctccgca agggagagga ctgcacggac ctggaccggg ccgcgacaga ggcctaccgc
    32101 cgccgcgtcc agatgatctt ccaggatccc tacgcctcca tgaatccgca ctacaagatc
    32161 agggacgtcc tggaggagcc cctcctcatc caccgcgtcg gcgggagccg ggcggagcgc
    32221 gcggccctcg cggaggaggt gatggccaag gtcaagatga cgcccgccgc cgagttcctg
    32281 gaccgccacc cccacatgct ctccggcggc cagcgccagc ggatcgcgac ggcgcgcacc
    32341 ctcatcctca agcccgagct catcgtcgcc gacgagcccg tctcgatgat cgacctctcc
    32401 acgcgggcgg agatcctcca cctcctcaag accctccagg gcgacatgga gctctccttc
    32461 ctctacatca cccacgacat ctcgacggcg cgctacttca ccgaccgcat cgccgtgatg
    32521 tacctggggc gcatcgtgga gatcggcgcc caggacgagg tgatcgacca tccccgccat
    32581 ccctacacga gggccctcat cgaggccgtc tgcgagcccg tccccgggcg cgtgggcgtg
    32641 aagcgcgcgc tcaacgtgac gggggagatc ccctccgcga ccgccgtgcc ctcgggctgc
    32701 cgcttccaca cgaggtgctg ctacgcgaag gaggagtgcg cctccctgcc cgagcccgag
    32761 ctccaggatt ccggaggcgg ccacctctgc gcctgcaggc ggtggaagga gatatagcat
    32821 gactcgcggc gggattctcg atagccggcc aggacgggtc ctg
//
LOCUS       DAOOSJ010000003        32145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1010_length_32145_cov_6.575506, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000003 DAOOSJ010000000
VERSION     DAOOSJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 32145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32145
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_32145_cov_6.575506"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PLG14_00335"
     CDS             <1..147
                     /locus_tag="PLG14_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HOX47601.1"
                     /translation="TSIQEQTLTFVPKIAAIMLVLALLGGWMLGHLAQYTRGLFAMIP
                     QMAK"
     gene            156..932
                     /gene="fliR"
                     /locus_tag="PLG14_00340"
     CDS             156..932
                     /gene="fliR"
                     /locus_tag="PLG14_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOX47602.1"
                     /translation="MGWPFGDWNLFLLIAVRVIAMIEVAPLLSTTSVPQVAKIGLAGF
                     AAAAVFPSVKAAGYPIPSDGAAYVLLLGGEAMIGVIMGFFLSILYSAFTTAGQFFSLQ
                     IGFGAMETFDPLAEIEIPVMGQFLNLIAMFIFVATEGFQKLFLVGVQGSFKAMRAVDL
                     VARREDLIQFLVRSTGALFQQALILSIPVLGTLFIITVALGLLSKAAPQMNLMTEGFP
                     ISLMVTFFIMLAALPLMMEAFSRIIESGFSDLARLVGGAL"
     gene            1082..2194
                     /gene="flhB"
                     /locus_tag="PLG14_00345"
     CDS             1082..2194
                     /gene="flhB"
                     /locus_tag="PLG14_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOX47603.1"
                     /translation="MDLQWFAAEDEGRTEEPSEYKIRKAREEGRVAKSQELVGALGLL
                     LPAVTLVLLAPYLGRTIREMVVFYFSKVSEADIAGGGGPLAAAFFSYFARLTLPLALV
                     AVVSGVASNLLQTGFLFTLKPITPDFSKIVPRFGQYFKRTLFSMEGLYNFAKTIFKVA
                     LIGVLAYSTVRSELDRLMALFAAPFWLSVKLVVSLAMRLVIEVAVILLALSIPDYIFQ
                     RRQYMESLKMTKEEAKEERKMQEGDPLVKSRMRDRMRELLSRNMAANVPKADVVIANP
                     THYAIALEWERERMAAPTVTAKGADEVALRIRRIAEEAGVPVVENRPLARALYAEVEI
                     GDSIPEKYYQAIAAVLAHVYAANGDAEELRREAAGA"
     gene            2206..4296
                     /gene="flhA"
                     /locus_tag="PLG14_00350"
     CDS             2206..4296
                     /gene="flhA"
                     /locus_tag="PLG14_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOX47604.1"
                     /translation="MADTQRVLRDTLLQNPSDIAVAVATISVILMIIIPVPAVLLDTL
                     LALNLTVAILIILIVLYTRKATEFSIFPTMLLVTTVFGLALNISSTRLILSKGANFDA
                     KLIRAFASFVVGTGGAEGLVIGFVIFVVIIAVQAIVITKGSTRIAEVAARFTLDGLPG
                     KQMAIEAEYNSGSISEEEAIKRKADVQREVDFYGQMDGASKFISGNVKVGIFISVINL
                     LGGFIIGMVLHGESFQVAIATYTALTIGDGLLAQLPALLISTATGIIVTRANSEGTFG
                     REVTAQFAANAKIYFVASAVLALLAILPGFPWYVLLPMAGLLAFVGFRLARKETDAAE
                     KARTEAASGGKKAEAPAELSPVVPLDPISLELGYGLIPLVDKDKGADLLERITRIRKE
                     SALDMGLVVPRIRIIDNMRLEPSEYNFKIKGVPVGRGVIRIGYYLAINPGGASDDVPG
                     EKTKDPTFGLPALWIAEENRDRAERAGYTVVDAPSIIATHLTEIIKRHASEILGRQEV
                     QAIMDALKKDYPAVVEDAQKRITLGETQKVLQGLLREQVSIRNMVAILETLADYAGVT
                     KDPGFLVEKARQALGRQICLQYADQDRSLRVLTIDPSLEQRIIESRVDAPMGAISALE
                     PATQRQWIKALTRAVAAVQAKGWFPVILCSEAARALVKSSTEREIPDLVVLSVPEIVA
                     EAKVEAVGEIRLEG"
     gene            4304..5587
                     /gene="flhF"
                     /locus_tag="PLG14_00355"
     CDS             4304..5587
                     /gene="flhF"
                     /locus_tag="PLG14_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOX47605.1"
                     /translation="MQYFTEQAPTHREALDRVRAKYGEQAQVLSHRTVRTGGLLGLFA
                     REAIEVTGYIRPASAQAARPQAKADLEEEKRKILERARAEQAKSDEALERVLDEIRDL
                     KTRIDDRGRASPEAEDHEAIRRADELLSQNDFTESFRRELVASLRRDFTLEGLEDWPE
                     VEAALLERIGDCVEVWREPPARSSRGPRVLALVGPTGVGKTTTIAKLAAIYAVGIGGE
                     KPKSVRMITIDNYRIGARQQIETYGNIMGVPVSCVETAEDLKKTLALHSDADLVLVDT
                     IGKSPRDAVRLAEMQRLVAACGPQAEVHLAVTATMKTSDMAETLRQFEPFGYRAVVLT
                     KLDETNRVGNAISVLAERRKALSYVTTGQRVPQDIERAAVPALLTRLEGFRIDRARLD
                     ARYGEEPGSRRAEQARQGRAAESGEDGGPERAERR"
     gene            5590..6495
                     /locus_tag="PLG14_00360"
     CDS             5590..6495
                     /locus_tag="PLG14_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOX47606.1"
                     /translation="MEDQAQALREIMGTKAAPAKAPAQAAPGAGPAGAGKKTRIIAVA
                     SGKGGVGKTNLSVNLAIAYAQLGKRVIVMDADLGLANVNVMLNMIPKYNLYHVIRKQK
                     TMREIILDTEYGIRIVAGASGFSKIANLTEEERQNFISELYTLSEADIVIIDTSAGVS
                     NNVLSFVAAADDAIIVTTPEPTAITDAYGIIKIIATEIENLNIGLKLVVNRVKSVTEG
                     RRVAERIINISGQFLNLKVEYLGCIYDDPVVPTGVVRQRPFLVAEPKSKAAISVQHLV
                     SRIEKTDFADDRGIGRFIKKLFGKD"
     gene            6582..7307
                     /locus_tag="PLG14_00365"
     CDS             6582..7307
                     /locus_tag="PLG14_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47607.1"
                     /translation="MDSDLRASLIASVAAAALSALVGAVAGVPFLAILLRAGAGGLAF
                     GALVYGAIALLRRFTPGLIDEGASGEAVGPAEAEEGPEPGSSVNIVLPAEGPGFDGRG
                     EEAFGADHGAEGGPAADGGLEELPAAPRPAAGASEDPGGYEFAAGSLVDAEDAEPVGA
                     RAAAGASPGPAESFDELDVLPDLESLSDSFEAAATRSMTRGADDDEGARRPRPTSSGG
                     DRNSDPAVLAQAVRTLLKRDEKG"
     gene            7314..8102
                     /gene="whiG"
                     /locus_tag="PLG14_00370"
     CDS             7314..8102
                     /gene="whiG"
                     /locus_tag="PLG14_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HOX47608.1"
                     /translation="MAENPHARLSEEELWPLYKRTRDPQIRDAFIRQYAPLVKYVAGK
                     VAAGMPNTVEFDDLVGFGVFGLLDAIEKFDPDKNVKFKTYAITRIRGAIFDELRSIDW
                     VPRSVRQKTKEIEEAVVSLESKLGRPASDTEIAGALGLSEDELLKTMLKISGTSVLSL
                     NDVWYSGDDSDKISIGESIESPSSLNPDASVERDEVKRVIVQAISELPEKEKKVLVLY
                     YYEDLTLKEIGQVLEVTESRVSQLHTKAILRLRAKLTNVRKGIL"
     gene            8106..10085
                     /locus_tag="PLG14_00375"
     CDS             8106..10085
                     /locus_tag="PLG14_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOX47609.1"
                     /translation="MARKLGLEEVASLMRRYLEEDEGKKSLVALGASLEEALTDAAVQ
                     LSVPVKLLEYEVVERGGSGVLGLGRKPWKIKAYVAAAKKEVEAPAPAEEDFSVFESMP
                     EKKDKDGECFVRLSPDGVLLKVTPPLGRGRRASERQAYDRIEARGARNYDEGLVKEAL
                     RQASGEYVKVGEFISNPANDALLVVDVTDQEMKAYVQLTPPGPGGCDLSKDSILSFLR
                     NNRVVHGVSEDALRDLEDKPRYREPVLVAEGSRPANGRDASVQFNFETDKSKHRLKEA
                     SDGRVNWKELGLIQNVVAGQPLARRLPPELGQAGRTVTGKTIPARNGKDAPPPLGRNV
                     HLADDGVTIIADMNGQVTFIGGKINVEEILTIQGDVSLKTGNVMFLGTVIVLGNVEDG
                     FSVKASGNVEVRGTVGKCEIIAEGDVVVHQGVAAKSGGIVQAGKNVWSKFIENAFVEA
                     GENLIVSDGIINTTAVANKKIICQGKRAAIVGGRYRACEEINAKTLGSPVGGAETLLE
                     VGYDPKSKEKLDQLQAQVGQLTRQLETLDKNIATLNAIKKQKKELPEEKEAVLQDYLA
                     RRDELMTELRAVAKEAESVQSYLNGLKTRGRISASGRVYPGVKICIKDVKEDIKTEQK
                     GITFFLENQMIRTTKYEEPEDEFLKRGPPDAYKAD"
     gene            10102..10398
                     /locus_tag="PLG14_00380"
     CDS             10102..10398
                     /locus_tag="PLG14_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47610.1"
                     /translation="MQMGQVGREKAAEKEGAILQASMKGAAEQKRADEAKEAVRRPTD
                     PEGGTPAVRDRKERPQGGREGRREGEAPAEEAEAKEEEEVVRDPSLGNKIDLSG"
     gene            10405..11154
                     /locus_tag="PLG14_00385"
     CDS             10405..11154
                     /locus_tag="PLG14_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47611.1"
                     /translation="MTSFLALLALDILGLAGLWLYVRARLRRALELEGLLGEIRKEVR
                     GLNIEINETADRNISLLEDRMDSLRSLLDEADRRMGVVRKELSARAAEREVYSHLGRP
                     RPRAGEEAQGGEGGYAGFAPAAPRPEPRPEPRPEPRAAGRPEPRPESRPEPRAAEGED
                     AELGLPEVAGAEEPIRLELSREPRRPPEIRRASESVIPPRPLRERAIELHRKGFSPDI
                     IAARLGATVAEIELLVSLEESLDERRGSAED"
     gene            11158..12972
                     /locus_tag="PLG14_00390"
     CDS             11158..12972
                     /locus_tag="PLG14_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOX47612.1"
                     /translation="MGPVIGIDFGTTNSLCAWLDGDRPAIIPNARGDRSTPSVAASTA
                     KGEILVGESAKNQALVNPDNTVAGVKRLLASGGLLSMGGKTWRPEEIAALILSSLKAD
                     AERHLGREVSQAVVAAPANFSERERRALLEAGRLAGLEVLRILNEPTAAALARAWAAG
                     SGSAGSAPRGVAAPSKAAPGGPARSSSAGQAGKAAAGAATESPGPQTILVYDFGGGTF
                     DVTVLRQDGPDCVVLSSRGDGRLGGADLDRELYRRAAEAFAAEYGLEVGADRRIAQAL
                     SEQAEKAKIELSERLEASMAIPFALATGADGQPRVVHPVFAVSREEFEAVALPYVERS
                     LALAERALKDAGLGAKDVDLLVLSGGSSRIPLVRRLLKERLGLEPAGGVDPEESVALG
                     AAVQASLLEGSERLRLRDVVSRTYGVEIDGGRFVPLIRKNSPVPASRSRVFTTVSEGQ
                     DSVEIHVLQGDAERAGDNLSLGRFLLSGLRFAKAGVPRIKVDFGIDESDILHVEAVDL
                     DTGAAQAISIADLDRGAGAEPPEALAEKARLLAGRLAELKDGLSLESGLEAELEEAAL
                     RAEGARRDAPEGELRLLKAELEGLVGELLARRGELARR"
     gene            12996..13607
                     /locus_tag="PLG14_00395"
     CDS             12996..13607
                     /locus_tag="PLG14_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOX47613.1"
                     /translation="MSEERLLAVLGLGPGAEEEEIRTAYRRLARRWHPDSSRDPGTAG
                     QFARVARAYKVLSARPPRSPAPMGGAAAKYRRVIEAGEDLFALGQVLASDPEAGAREA
                     AARRLGLSGRTAAYVFLRRALYDRSEEVAAAAVRSVALLGARQAEGEVAALYSRATPG
                     LKRLVLETALATREPLFKAAIRAASKDEDPALRVLAARAAAGE"
     gene            complement(13736..14617)
                     /gene="sdaAA"
                     /locus_tag="PLG14_00400"
     CDS             complement(13736..14617)
                     /gene="sdaAA"
                     /locus_tag="PLG14_00400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOX47614.1"
                     /translation="MNWKFSTALELLDRASEAGLSLPAYLLARDSFHSGKSEEELRAE
                     LARRIRVSREALDRGIREPQRSFSGMTDGAAAKLAHGEAWPVDDELFRRALVYALSIN
                     EVNACGGRIVAFPTAGSCGIAPGVLWAFRDARAPELGPDSPRFQDAFVVASAVGILIA
                     LRATLAGAAGGCQAECGAAAAMAAAGLAHLLGEDAEGAFDAAALSLKNSLGLACDPVA
                     GLVEVPCAKRNAFMAANALTAVCLRRAGIRSVIPFDEVVGAMKAIGDAMPASIRESAE
                     GGLAVTATGLLFREKIK"
     gene            complement(14630..15313)
                     /gene="sdaAB"
                     /locus_tag="PLG14_00405"
     CDS             complement(14630..15313)
                     /gene="sdaAB"
                     /locus_tag="PLG14_00405"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOX47615.1"
                     /translation="MADRNVSLLDIFGPVMIGPSSSHTAGVARIAFLARKIFPYPLDG
                     ARVSFYGSLARTYKGHGSDKAAVAGLLGILPEDERLGYALELAAREGLAVEIVPVYDG
                     PERYHPNTAVVELWSGDRRIRIRGASIGGGEIRLQSIGGFEADLACSLDAILVLHRDE
                     VGVIAIVSHILAANRFNIAGVASHRKDKGDEALLVVEVDGVVPASIVAQIRELPAVRE
                     VVYVPSIRS"
     gene            complement(15370..16755)
                     /locus_tag="PLG14_00410"
     CDS             complement(15370..16755)
                     /locus_tag="PLG14_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX47616.1"
                     /translation="MRATILVVDDEKNIREGLAEALSLDGHAVRTAADGAEALKAVEE
                     SLVDLVITDLRMPALSGSELLKTLASRYPGLPVIVLTGHGTIEDAVEAMRYGAFDFLT
                     KPVNLDHLSMLAKRALERGELSRKNRELEAEIEAQRRSSSIIGSSQEMKRVFELVRRV
                     APTKASVLVTGESGVGKELVADAIHNLSPRRDGPFVKVHCAALAESLLESELFGHEKG
                     SFTGAQARKRGRFELADKGSLFLDEIGEINQNVQIKILRVLQERRFERVGGEETVETD
                     VRIVAATNRDLKKEIAEGRFREDLYYRLNVVNIHVPPLRERRDDIPLLAMAFLKEFAG
                     ENGKSVEGFDPKARSALYAYGWPGNVRELRNCVESAVVMARGGLVGLDDLPPTLRSSG
                     EDRDIRVPAGSSLAEAEKILIRETLAAQNGNKSRSAEILGIGRKTLYQKIEEYGIEAP
                     GAAEKAPGPEA"
     gene            complement(16756..17946)
                     /locus_tag="PLG14_00415"
     CDS             complement(16756..17946)
                     /locus_tag="PLG14_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX47617.1"
                     /translation="MRRFVERALAKAPRMNEEQLRGLLQALAEENGRLEAALDSMLDG
                     IVVCDRDHLPTIYNKSAERMLGLAGGEPSERPLWLSVADRELAEFLRDTLEGEESVLD
                     REFELDAPGGRTRLVAASATTLVARGRLEGTLLHLEDITDKRRREAQLRRAESLASLT
                     TLAAGVAHEIKNPLGSISIHIQLIKKALKSGKPLVPETMDRHLGVVTEEIERLNKIVV
                     DFLFAVRPMDVELREADPRVLVREVADFLRYEADAAGVEIETSLEEEVPRVLLDKRYL
                     KQALLNLAKNALAAMPDGGRLGLSVEEKDGEVLIAVEDSGTGIPEEYLPKIFEPYFTT
                     KEAGTGLGLTITFKIVKEHDGAIDVRSRPGQGSTFTIALPVPQKERHLLPWKDAEGAA
                     PRRD"
     gene            complement(17943..19343)
                     /locus_tag="PLG14_00420"
     CDS             complement(17943..19343)
                     /locus_tag="PLG14_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47618.1"
                     /translation="MFENLLGQDEAAGRLKADLEAASLPPSLLFAGPPASGKQSAALE
                     LARVLSCERGPLGLPPGPAPWNCPCPSCSRHRVLAHPDLLLLGPRSFPEEIQAGLGLL
                     ERAPGRASAFFFVRAARKLGKRFDAALYEGEESRLSKALPLLREIEERLDAVAPERAE
                     AGSLAPGAVEAARAAAQAARKLEGLVPDAPPVFQVRSAETWARLAPNGRKKTIVVENA
                     DRMAEAARNALLKILEEPPQSVEFILLSARPSALIATVLSRVRPYVFKERSPAEARLV
                     LDRVFKLGQGAPAAAEAAAPGAAGGAQAGAAAGGPGGSAPPAPTGVAAYLASLRAFPP
                     EAARARAGDFLAAALALRASRGPLEGGLAACAAGLSAQGEAPGPAEALQGLLEATKDF
                     GQKDEAYEGSFDAFLAALAGRFGELLREPGLDAAGLLLVEGWTALAREARSRRATYNL
                     SPSLLAESLLYAMGDR"
     gene            complement(19352..19912)
                     /locus_tag="PLG14_00425"
     CDS             complement(19352..19912)
                     /locus_tag="PLG14_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOX47619.1"
                     /translation="MNALDWILLAILLLLGLRCLVRGFVQELLSVAAYAVGLGAALYL
                     YKPAGAFLAGRFAGLPLPAVIAFAALFLIGFLLTRLLGKLLKEGLEAANLAWADKVLG
                     FVIGLAEGFVVVAVILLVLKVQPLFDVKPLLSDSVFAKALLPILEPGVAKAIGGAANG
                     ALPQKAAPKVPEALKKLAPPAPASKP"
     gene            complement(19909..21030)
                     /gene="murG"
                     /locus_tag="PLG14_00430"
     CDS             complement(19909..21030)
                     /gene="murG"
                     /locus_tag="PLG14_00430"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOX47620.1"
                     /translation="MGDDRVIAFTGGGTGGHIYPGLAVIERLKGRFGGRIVWIGSAKE
                     SDRRVVEAAGVEYYAVPSGKLRRSFSLENAADGFRVLAGYLASRRLLSELRPVLLFSK
                     GGYVSVPPCAAAAALGIPVFTHESDLTPGLATRLNARRAERVLVSYEKTVGLLSPELR
                     GRAVLVGNPVRASIRSGDAGRGRAFVGAPDGVPVILFLGGSQGARQVNELVAAALPEL
                     AKLAYVVHQTGEAQAGSGAGAECAAASKGRYRPYAYIHGEMADVVAAADLVVGRAGAG
                     TLWECSALGKPMILVPLAGSGTRGDQVDNARLFEAAGAARVLLGADATPARLVEEARS
                     FLADPARLARAGEAARALAGSDAAGKAADLILERIGALS"
     gene            complement(21035..22657)
                     /gene="pgi"
                     /locus_tag="PLG14_00435"
     CDS             complement(21035..22657)
                     /gene="pgi"
                     /locus_tag="PLG14_00435"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526422.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOX47621.1"
                     /translation="MAEFRNLDACEAYKALLAYREPPRLETLLDAARVRAAEVPMGGG
                     LSYNWAACPVDAPRLALLERLAAEQELVAKFRLIASGEVMNPGEKRMVLHHLARGELS
                     GPVVKDGRDYRAFYEGERRKIADFAKAVHDGSVRGSSGKVFTTVVQVGIGGSDLGPRA
                     LYLALERWAKAVGRFKMAARFISNVDPDDADSVLSQIDPAASLFILVSKSGTTQETLA
                     NETLVRARLQALGLDPARHIVAVTSETSPLARNPAYAASFYMDDYIGGRYSSTSAVGG
                     AVLSLALGPETFAELLAGAHEEDLLALEPALGKNPALLDAMIGLYERNVLGLPATAVL
                     PYAEPLSRFPAHLQQLDMESNGKRVNRSGEPVAYATGPLVFGEPGTNGQHSFYQLLHQ
                     GTDIVPLQFIGFRENQVSDDVSSEGSTSRAKLNANLAAQILAFAAGKADPNGNKHFPG
                     QRPSSLIHGRRLDARALGALLAHFENKVAFQGLAWNLNSFDQEGVQLGKVLAKKVLAG
                     VSPEAAGTEGALGAYAGLLGLGAAGAGAGGKA"
     gene            22829..24040
                     /locus_tag="PLG14_00440"
     CDS             22829..24040
                     /locus_tag="PLG14_00440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX47622.1"
                     /translation="MAARIERVEKEFILGAAAEAGTLARIQASGRTVDCRLVQAGKES
                     LRFSPAEEDSALFKAWDRVAVRFDFRGQTVAFSAPVRKAARGLLELAWPEAMYRGLSR
                     RFARVAAPRDFCADLLLPDLDLRLGCPECREYAEVERPAAKEGLDADTLASLVAAFKE
                     KAAFHASESRVVMWKDGRGPADAAEDLVANLGRILYIPSTLSGLPLADPYPEGRVITR
                     AMLEDYESPASLAGDSPLGEKLAALSREGLAAALWCPILYYRYAVGVVYLGNDGASRK
                     PFDLRAVDFAWEFSRLLAWLLKRHGYFGAAEAEPQARRGAVVDASPSGALIEISGRAP
                     RLKQGSAVELLLSLGDKSYSCRGRVARRWEDKGSSFYGLAFEGLAEEARAELERGLYG
                     VLSPEAPGGRS"
     gene            24042..24263
                     /locus_tag="PLG14_00445"
     CDS             24042..24263
                     /locus_tag="PLG14_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47623.1"
                     /translation="MAAFLSLALSVLKDWRVIAATVVILLAWSLFRYVGLVYRKVRNY
                     GALPKSARGRKGGKAPAPAPAVEEEGEGA"
     gene            24420..25727
                     /locus_tag="PLG14_00450"
     CDS             24420..25727
                     /locus_tag="PLG14_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX47624.1"
                     /translation="MANKTPMNGYGSLAKRINLFPQGAPPGELLFRILSVLFSEKEAA
                     LLALLPLRPFTAAAAARLWKLPEAGATALLEDLASRGMILDMETEGGRIFTMPPPMAG
                     FFEFSMMRVGGRYDQRFLAELYHQYLNVEEDFVRELFAGGETQLGRVFVGEKALAERP
                     AAAGAVSVLDHERASEVARTATHRGVGTCYCRHKMSRLGKACGAPMEICLTFNGVADS
                     LVRHGVARGIDEAECLDLLSEAEERGLVQFGENVRESVSFICNCCGCCCEALLAAKRF
                     ALLKPVATTAYLPAVDLAACSGCGRCVEACPVESMALVSASDPRSPRRKKARVDESTC
                     LGCGVCVRACPGGALSLAFRGARLVTPVNSAHRAVLMAIERGKLQNLVFDNQALASHR
                     AMAAVLGAILRLPPAKRLMASRQMKSVYLERLLSRKEFVRREG"
     gene            complement(25724..27247)
                     /locus_tag="PLG14_00455"
     CDS             complement(25724..27247)
                     /locus_tag="PLG14_00455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOX47625.1"
                     /translation="MEEEGEDPVARLARERFGVPWLYPLQRLAVANVLDAAESEEGET
                     GRQLVLLPTGAGKSLCFQLPALVLPRPTIVVYPLLALMEDQRRSLARLGIPYALFRGG
                     QEESERRAAMADLESGRAKLAITNPESLGSSGLLDFLASLKPSHLAVDEAHCVSEWGE
                     TFRPAYLELGEIAERLAPRALSAFTATASPSVVEALERLLFRGEGYRLVSGDPDRPNI
                     SYSVARTLSRNRSLERLCRGLPKPLIVFVSSRAGAEILAELLRARLGSEDIRFYHAGL
                     EREEKREVEAWFYGSATGILCATSAYGMGVDKRDIRSVVHYEAPASVEAYLQEAGRAG
                     RDGLPSRAVLVAGRDDGARLGREADPGRRARRAALLAYAAGEGGCRRESLLRLLGAEP
                     RGPCSGCDRCEGSAEEGFEGGAELAAFARLNSRRFSEKEALDLLLGTRGLEPPLAAGS
                     GSMEGWRREDAASALRAAAELGLIRLVGKGPWKGKLAGPRRGPRQGVGPSSRRVPAS"
     gene            27406..28281
                     /locus_tag="PLG14_00460"
     CDS             27406..28281
                     /locus_tag="PLG14_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47626.1"
                     /translation="MVDAHLEGEDWDEPHEAMAFESSYGGVIETLKELVAGLRAAEAG
                     EGPRPPHEELAARSRALLVRAPALVNVMLNYKICAEHGLPLHPTVYYELAEARRYRLA
                     YPIAEIERANRLFRDSIDLARAAYGLAGDFEARAEAFRSRLPPEVQDFVYTGTRDKYT
                     WRASEPAKVRALARVVREGFNPGLVVAAAHGSIMPGLLLAEYLDRPLYFLRFSMFKRK
                     DEAPILSIADEAWLAERSEERALLFDEDVAKGTTLAIFAERLKHLFRESRTACVIRHA
                     GSGLRPDFAARTWWD"
     gene            complement(28374..29045)
                     /locus_tag="PLG14_00465"
     CDS             complement(28374..29045)
                     /locus_tag="PLG14_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47627.1"
                     /translation="MALFELFDTLDKGIKKDLLSGREKDRALARLAKTRARYEALGEI
                     DETGGVGAGLVERGRKLLSASPLKAEQAEKFFRHANAAWYDLSGRLGAYTWYVRCFFA
                     ATIGFFVLSPQFFGPMLALIFLVPTFLGLRGLKTRSVSGFTLSAMNFPVALLASTTAS
                     RFFVPAAFGNLGQAAAELAAKYPPLPVGAASALVVVFALLSVVTIVASVAGAVVGFLN
                     KDMFV"
     gene            complement(29222..30796)
                     /locus_tag="PLG14_00470"
     CDS             complement(29222..30796)
                     /locus_tag="PLG14_00470"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOX47628.1"
                     /translation="MSETKRENWGSKLGLILAMAGNAVGLGNFWRFPYQAAKNGGGAF
                     MVPYFIALIFIGIPLMFVEWNLGKTGGKYGHGTLGPMVYLQARDKLKPKSALGVGALA
                     GGLAFAVVILVNSYYNQIIGWTLGYTWLSLTGGYKDAAVSTVDVFLNYIGNPVNMIFW
                     VIALAALGFAVMRGIQKGIEAWARVMMPLIYVFGVILIVKALTLGSPVNPDWSPIKGL
                     EFVWTPDFKSLNFGSALAATGQIFFTLSLGMGIISNYASYLKADDDIVLSGFTTIALN
                     EFAEVILGGTIAIPIAFAFLGPDGIGQSVGLSFISLPNVFRNMAGGQFFGGFWFLLLF
                     FAGFTSAIAMYNYLVALLEEDMGIKRSTGALIVFALYIIVGLPVGIEPTLIRGSTAYL
                     DAVDFWVGNVFLVVLGLIEIVVTGWILGKKGLDQINKGSYWKLPKWYYTLFIQVAAPV
                     LIIGFLVGYVVYLAGGGAVLGNSLKNIMSASPWTMAGQIVVLAVLVLGVIQTNLSIRK
                     KYPEEIAKNTVLVVKE"
     gene            31365..32009
                     /locus_tag="PLG14_00475"
     CDS             31365..32009
                     /locus_tag="PLG14_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOX47629.1"
                     /translation="MAKIKVSYAPSVRRLPSYLHIIRQSQRAGDYYISGTVIAQELNL
                     EPIQVRKDLAITGIIGKPKKGYPVEALVAAIEHYLGWDQSHDAVLVGAGNLGSALLGY
                     QEFQLHGLHFVAAFDTDPAKIGQHIHGVPILSMDTIEQGIKSLGAEIAVLAVPSPNAQ
                     ATGETLVRAGITSIWNFTNTKLKVPDEIIVQREDLSSGYALLSVMMQNRKGRLV"
     gene            32006..>32145
                     /locus_tag="PLG14_00480"
     CDS             32006..>32145
                     /locus_tag="PLG14_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA (guanine-N2)-methyltransferase"
                     /protein_id="HOX47630.1"
                     /translation="MTDKDARQAEYFANRLAKNEKNLRRWARREGAGAVRLYDRDIPE
                     VPL"
BASE COUNT         4923 a        11275 c        11373 g         4574 t
ORIGIN      
        1 acctcgatcc aggagcagac cctcaccttc gtcccgaaga tagccgccat catgctcgtc
       61 ctggccctcc tcgggggctg gatgctcggg cacctggccc agtacacgcg ggggctcttc
      121 gcgatgatcc cccagatggc gaagtgagga cctaggtggg ctggcccttc ggcgactgga
      181 acctcttcct cctcatcgcg gtccgcgtga tcgcgatgat cgaggtcgcg cccctcctgt
      241 cgacgacgag cgtgccccag gtcgcgaaga taggcctggc cggcttcgcg gcggccgcgg
      301 tcttcccctc cgtgaaggcg gcgggctacc ccatcccctc cgacggcgcg gcctacgtcc
      361 tcctcctggg cggcgaggcg atgatcggcg tcatcatggg cttcttcctc tcgatcctct
      421 actcggcctt caccacggcc ggacagttct tctccctcca gatcggcttc ggcgcgatgg
      481 agaccttcga tcccctggct gagatcgaga tcccggtcat gggccagttc ctcaacctca
      541 tcgcgatgtt catcttcgtc gcgaccgagg gcttccagaa actcttcctg gtcggcgtcc
      601 agggctcctt caaggccatg cgcgcagtcg acctcgtggc caggagggag gacctgatcc
      661 agtttttggt gcgctccacg ggggccctct tccagcaggc cctcatcctc tccatccctg
      721 tcctcggcac cctcttcatc atcacggtcg ccctaggcct cctatccaag gccgcccccc
      781 agatgaacct catgacggag ggcttcccca tcagcctcat ggtgaccttc ttcatcatgc
      841 tggccgccct tcccctgatg atggaggcct tctcccgcat catcgagtcc ggattctccg
      901 acctcgcccg actggtcggg ggcgccctgt gatcctcctc ttcccggcgt cgcggggcgc
      961 gggcccggcc gccggcgcgg cccctgccgc ctcggacctc cgctccaagg ccgcggacct
     1021 cggagccgcc gatctccgcg ccgccggccg ccgctccggc gccgcgcgga gcccggcttc
     1081 gatcgacctg caatggttcg cggccgagga cgaggggcgg acggaggagc cctccgagta
     1141 caagatcagg aaggcccgcg aggaaggccg cgtcgcgaag agccaggagc tcgtcggggc
     1201 cctgggcctc ctcctccccg ccgtcaccct cgtcctcctc gccccctacc tgggcaggac
     1261 gatccgggag atggtcgtct tctacttctc gaaggtctcg gaggccgaca tcgcgggcgg
     1321 gggcggcccc ctggccgcgg ccttcttctc ctatttcgcc cgcctcaccc tgcccctggc
     1381 cctcgtggcc gtcgtctcgg gcgtggcctc caacctgctg cagacgggct tcctcttcac
     1441 gctgaagccc atcacccccg acttctccaa gatcgtgccg cgcttcgggc agtacttcaa
     1501 gcggaccctc ttctccatgg aggggctcta caacttcgcg aagacgatct tcaaggtcgc
     1561 gctgatcggc gtcctcgcct actcgacggt gaggagcgag ctggaccgcc tcatggccct
     1621 cttcgcggcc cccttctggc tctccgtcaa gctcgtcgtg tcgctggcca tgcggctcgt
     1681 catcgaggtg gccgtcatcc tcctggccct gtcgataccc gactacatct tccagcgccg
     1741 ccagtacatg gaatccctca agatgacgaa ggaagaggcc aaggaagagc gcaagatgca
     1801 ggagggcgac cccctcgtga agtcgaggat gcgcgaccgg atgcgggagc tcctctcccg
     1861 caacatggcg gccaacgttc ccaaggcgga cgtcgtcatc gccaacccga cccactacgc
     1921 gatagccctg gagtgggagc gcgagaggat ggcggcgccc accgtcaccg cgaagggcgc
     1981 cgacgaggtg gccctgcgga tccgccgcat cgccgaggag gcgggggtcc ccgtcgtgga
     2041 gaaccgcccc ctcgcccggg cgctctacgc cgaggtcgag atcggggact ccatccccga
     2101 gaagtactac caggcgatcg ccgcggtgct ggcgcacgtg tacgccgcca acggcgacgc
     2161 cgaggagctc cgccgcgagg cggcgggagc ataggagcat agctgatggc cgatacgcag
     2221 cgcgtgctca gggacacgct tctacagaat ccctccgaca tcgcggtggc cgtcgccacg
     2281 atctcggtga tcctcatgat catcatcccg gtcccggcgg tgctcctcga cacgctcctg
     2341 gccctgaacc tgacggtcgc gatcctcatc atcctcatcg tcctctacac caggaaggcc
     2401 accgagttct cgatcttccc gaccatgctc ctcgtgacga cggtgttcgg cctggccctc
     2461 aacatctcgt ccaccaggct catcctctcc aagggcgcca acttcgacgc caagctcatc
     2521 cgggccttcg cgagcttcgt cgtcggcacg ggcggcgccg agggcctcgt catcggcttc
     2581 gtgatcttcg tcgtcatcat cgcggtgcag gccatcgtca tcacgaaggg ctccacgcgc
     2641 atcgcggagg tcgcggcccg cttcaccctg gacggcttgc cgggcaagca gatggccatc
     2701 gaggcggagt acaactcggg ctccatcagc gaggaggagg ccatcaagcg gaaggccgac
     2761 gtccagcgcg aggtcgactt ctacggccag atggacggcg cctcgaaatt catctcgggc
     2821 aacgtcaagg tcggcatctt catctccgtc atcaacctcc ttggcggctt catcatcggc
     2881 atggtcctgc acggcgagag cttccaggtc gccatcgcga cctacaccgc cctcaccatc
     2941 ggcgacggcc tcctcgccca gctcccggcc ctcctcatct ccacggccac cggcatcatc
     3001 gtgacgcgcg ccaactcgga gggcaccttc gggcgcgagg tgacggccca gttcgccgcg
     3061 aacgcgaaga tctacttcgt ggcctccgcc gtcctagccc tcctcgcgat cctccccggc
     3121 ttcccctggt acgtcctcct cccgatggcc gggctcctgg ccttcgtggg cttccgcctg
     3181 gcgcggaagg agacggacgc tgcggagaag gcgaggacgg aggcggcctc cggcggcaag
     3241 aaggccgagg cgcccgccga gctctcgccc gtcgtccccc tggacccgat cagcctggag
     3301 ctcggctacg gcctcatccc cctggtcgac aaggacaagg gcgcggacct cctggagcgg
     3361 ataacgcgca tcaggaagga gagcgccctg gacatgggcc tcgtcgtgcc gcgcatcagg
     3421 atcatcgaca acatgcgcct ggagccctcg gagtacaact tcaagatcaa gggggtgccg
     3481 gtcggccgcg gcgtcatccg catcggctac tacctcgcga tcaacccggg cggggcctcc
     3541 gacgacgtcc ccggcgagaa gaccaaggac ccgaccttcg gcctgccggc gctctggata
     3601 gcggaggaga accgcgacag ggccgagcgc gcgggctaca cggtcgtcga cgcccccagc
     3661 atcatcgcga cccacctcac cgagatcatc aagcggcacg cctccgagat cctcgggcgc
     3721 caggaggtcc aggcgatcat ggacgccctc aagaaggact acccggccgt cgtggaggac
     3781 gcgcagaagc gcatcaccct gggcgagacc cagaaggtcc tccagggcct cctccgggag
     3841 caggtgtcga tccgcaacat ggtcgccatc ctggagaccc tggcggacta cgcgggcgtc
     3901 acgaaggatc ccggcttcct cgtggagaag gcaaggcagg cgctcggaag gcagatctgc
     3961 ctccagtacg ccgaccagga ccgctccctg cgggtgctga cgatagaccc ctcgctggag
     4021 cagcgcatca tcgagagccg ggtcgacgcc ccgatgggcg cgatctcggc cctggagccc
     4081 gcgacgcagc ggcagtggat caaggcgctc acgagggccg tggccgcggt gcaggccaag
     4141 ggctggttcc ccgtcatcct ctgctcggag gcggcgaggg ccctcgtgaa gtcgagcacg
     4201 gagcgcgaga tccccgatct cgtcgtcctc tccgtgcccg agatagtcgc cgaggcgaag
     4261 gtcgaggcgg tgggcgaaat caggctggaa ggatagagag gatatgcagt atttcaccga
     4321 gcaggccccg acgcaccggg aggccctgga ccgggtcagg gccaagtacg gggagcaggc
     4381 gcaggtcctc tcccaccgca ccgtcaggac gggcggcctc ctcgggctct tcgcccggga
     4441 ggcgatcgag gtgacgggct acatcaggcc cgcctccgcc caggcggctc ggccccaggc
     4501 caaggccgac ctcgaggagg agaagcgcaa gatcctcgag cgcgcccgcg cggagcaggc
     4561 caagtcggac gaagcgctgg agcgcgtcct ggacgagatc cgcgacctca agacccgcat
     4621 cgacgaccgc ggcagggcct cccccgaggc ggaggaccac gaggcgatcc gccgggccga
     4681 cgagctcctc tcccagaacg acttcaccga gtccttccgg cgcgagctcg tcgcctcgct
     4741 gcgccgcgac ttcaccctgg agggcctgga ggactggccc gaggtggagg ccgcgctcct
     4801 ggagcggata ggcgactgcg tcgaggtctg gcgggagccg ccggcccgct cctcccgggg
     4861 gcccagggtc ctcgccctgg tgggcccgac gggggtgggc aagacgacga cgatcgcgaa
     4921 gctcgcagcc atctacgccg tcggcatcgg cggggagaag cccaagtcgg tgcggatgat
     4981 caccatcgac aactaccgca tcggcgcccg gcagcagatc gagacctacg gcaacatcat
     5041 gggcgtgccc gtctcctgcg tggagacggc ggaggacctg aagaagaccc tggccctcca
     5101 ctcggacgcc gacctcgtgc tcgtggacac gatcgggaag agcccgcggg acgccgtgcg
     5161 cctggccgag atgcagcgcc tcgtcgcggc ctgcggcccc caggcggagg tgcacctcgc
     5221 ggtgacggcg acgatgaaga cgagcgacat ggccgagacc ctgaggcagt tcgagccctt
     5281 cggctaccgg gccgtcgtcc tcacgaagct ggacgagacg aaccgggtgg gcaacgcgat
     5341 ctcggtcctg gccgagcgcc ggaaggccct ctcctacgtg acgaccggcc agcgcgtgcc
     5401 ccaggacatc gagcgcgcgg ccgtgcccgc cctcctcacg aggctggagg gcttcaggat
     5461 cgaccgggcc cggctcgacg ccaggtacgg cgaggagccg ggctcgcggc gcgccgagca
     5521 ggcccgccag ggccgcgcgg ccgagagcgg cgaagacggc ggccccgagc gggcggaacg
     5581 gagatgagca tggaagacca ggcacaggcc cttcgcgaga tcatgggcac caaggccgcc
     5641 cccgcgaagg cccccgcgca ggcggccccg ggcgccggcc cggcgggagc gggaaagaag
     5701 acgaggatca tcgccgtcgc gagcggcaag ggcggggtgg ggaagaccaa cctctccgtg
     5761 aacctggcga tagcctacgc ccagctaggg aagcgggtca tcgtgatgga cgcggacctc
     5821 gggctcgcga acgtcaacgt catgctcaac atgatcccga agtacaacct ctaccacgtg
     5881 atccgcaagc agaagacgat gcgcgagatc atcctggaca ccgagtacgg catcaggatc
     5941 gtcgccggcg cctccggctt ctccaagatc gccaacctga cggaggagga gcgccagaac
     6001 ttcatcagcg agctctacac cctctccgag gcggacatcg tcatcatcga cacgagcgcc
     6061 ggcgtctcca acaacgtcct ctccttcgtg gccgccgccg acgacgccat catcgtgacg
     6121 accccggagc cgacggccat caccgacgcc tacggcatca tcaagatcat cgcgaccgag
     6181 atcgagaacc tcaacatcgg cctcaagctg gtcgtgaacc gggtgaagtc ggtcacggag
     6241 gggcggcgcg tcgcggagcg catcatcaac atctcgggcc agttcctgaa cctcaaggtg
     6301 gagtacctgg gctgcatcta cgacgatccc gtcgtgccga ccggcgtcgt ccgccaacgt
     6361 cccttcctgg tcgccgagcc caagtccaag gccgcgatct cggtccagca cctcgtgagc
     6421 cgcatagaga agacggattt cgccgacgac cgcgggatcg ggcgcttcat caagaagctt
     6481 ttcggcaagg actaggggcg ccctgggcgc tccggaaagg tcgaacttga cgagtggtcg
     6541 gatttcggcc tactcttggg ggaatggggg gagggcctcg ggtggacagt gacctgcgcg
     6601 catcgctcat cgcctccgtc gccgcggccg cgctttcggc cctggtcggc gcggtcgccg
     6661 gcgtgccctt cctcgcgatc ctcctccgcg ccggcgccgg gggcctggcc ttcggcgccc
     6721 tggtctacgg cgcgatcgcc ctcctgcgcc gcttcacgcc cggcctcatc gacgaaggcg
     6781 cctccggcga ggccgtcggc ccggccgagg ctgaggaggg tccggagccc ggctcgtccg
     6841 tcaacatcgt cctccccgcg gaagggcccg gcttcgacgg gcgcggcgag gaggccttcg
     6901 gggcggacca tggcgccgaa gggggccccg cggccgacgg cggcctcgag gagctccccg
     6961 ccgccccccg cccggcggcc ggcgcgtccg aggacccggg cggctacgaa ttcgcggcgg
     7021 ggagcctggt cgacgccgag gacgccgagc ccgtcggcgc cagggcggcg gcgggggcct
     7081 cgccggggcc cgcggagtcc ttcgacgagc tcgacgtgct gcccgacctc gagtccctgt
     7141 ccgactcctt cgaggcggcc gccacgcgga gcatgacgag gggcgcggac gacgacgagg
     7201 gcgcccggcg cccccgcccg acgagctcgg gcggggaccg caattcggat ccggccgtcc
     7261 tcgcgcaggc ggtcaggaca ttgcttaaac gtgacgagaa agggtgagcg aagatggccg
     7321 agaacccgca cgcgcggctg agcgaagaag agctgtggcc cctctacaag cggacgaggg
     7381 atccgcagat ccgcgacgcc ttcatcaggc agtacgcgcc cctcgtcaag tacgtggcgg
     7441 ggaaggtggc ggcgggcatg ccgaacaccg tcgaattcga cgacctggtc ggcttcggcg
     7501 tcttcggcct cctggacgcg atcgagaagt tcgaccccga caagaacgtg aagttcaaga
     7561 cctacgcgat cacccgcatc cgcggcgcca tcttcgacga gctcaggtcc atcgactggg
     7621 tcccccgctc ggtcaggcag aagaccaagg agatcgagga ggccgtcgtc agcctcgagt
     7681 ccaagctcgg ccgcccggcc tcggacacgg agatcgccgg cgccctcggc ctctcggagg
     7741 acgagctcct caagaccatg ctcaagatct cagggacctc cgtcctctcc ctgaacgacg
     7801 tctggtactc gggcgacgac tccgacaaga tctccatcgg cgagagcatc gagtccccct
     7861 ccagcctcaa ccccgacgcc tccgtggagc gcgacgaggt gaagcgggtc atcgtgcagg
     7921 ccatcagcga gctgcccgag aaggagaaga aggtcctcgt cctctactac tacgaggatc
     7981 tcacgctcaa ggagatcggc caggtcctcg aggtcaccga gtcccgcgtg agccagctcc
     8041 acaccaaggc catcctcagg ctccgcgcca agctcaccaa cgtccggaag ggcatcctct
     8101 aggccatggc ccggaagctc ggcctggaag aggtcgcctc cctcatgcgg cgctacctcg
     8161 aggaggacga gggcaagaag agcctcgtcg ccctgggcgc gagcctggag gaggcgctga
     8221 ccgacgcggc cgtccagctc tccgtccccg tgaagctcct cgagtacgag gtggtcgagc
     8281 gcggcggctc cggcgtcctg ggcctgggcc gcaagccctg gaagatcaag gcctacgtcg
     8341 ccgcggccaa gaaggaagtg gaggcgcccg cgcccgccga ggaggacttc tccgtcttcg
     8401 agtcgatgcc ggagaagaag gacaaggacg gcgagtgctt cgtccgcctg tcgcccgacg
     8461 gcgtcctcct caaggtgacc ccgcccctcg ggcggggccg gcgcgcctcg gagcggcagg
     8521 cctacgaccg gatcgaggcc cgcggggcca ggaactacga cgagggcctc gtgaaggagg
     8581 ccctgcgcca ggcctcgggc gagtacgtca aggtcggcga gttcatctcc aaccccgcca
     8641 acgacgccct cctcgtcgtc gacgtgaccg accaggagat gaaggcctac gtccagctca
     8701 ccccgccggg gcccggaggc tgcgacctct ccaaggactc gatcctctcc ttcctccgga
     8761 acaaccgcgt cgtccacggc gtgtccgagg acgccctgcg ggacctggag gacaagccgc
     8821 gctaccgcga gcccgtcctc gtcgccgagg gctcgaggcc cgcgaacggc cgcgacgcct
     8881 cggtccagtt caacttcgag accgacaagt ccaagcaccg gctcaaggag gcctccgacg
     8941 gccgcgtcaa ctggaaggag ctcggcctca tccagaacgt cgtggccgga cagcccctcg
     9001 cccgccgact cccgccggag ctcggccagg ccggccgcac ggtgacgggg aagaccatcc
     9061 ccgccaggaa cggcaaggac gccccgccgc ccctggggag gaacgtccac ctcgcggacg
     9121 acggcgtcac catcatcgcc gacatgaacg gccaggtcac cttcatcggc ggcaagatca
     9181 acgtggagga gatcctcacg atccagggcg acgtctccct gaagacgggc aacgtcatgt
     9241 tcctgggcac cgtcatagtc ctcggcaacg tggaggacgg cttctcggtc aaggcctccg
     9301 gcaacgtcga ggtgcggggc accgtcggca agtgcgagat catcgccgag ggcgacgtgg
     9361 tcgtccacca gggcgtggcg gccaagtcgg gcggcatagt ccaggcgggg aagaacgtct
     9421 ggtccaagtt catagagaac gccttcgtgg aggccggcga gaacctcatc gtctccgacg
     9481 gcatcatcaa cacgacggcg gtggcgaaca agaagatcat ctgccagggc aagcgcgcgg
     9541 ccatcgtcgg cggccgctac cgcgcctgcg aggagatcaa cgccaagacc ctcggctcgc
     9601 ccgtcggcgg ggcggagacc ctcctcgagg tcggctacga ccccaagagc aaggagaagc
     9661 tggaccagct ccaggcccag gtgggccagc tcacccgcca gctcgagacc ctggacaaga
     9721 acatcgcgac cctcaacgcg atcaagaagc agaagaagga gctccccgag gagaaggagg
     9781 ccgtcctcca ggactacctg gcgcgccggg acgagctcat gacggagcta cgcgccgtcg
     9841 cgaaggaggc ggagtccgtg cagtcctacc tgaacgggct caagacccgc gggcgcatca
     9901 gcgcctccgg ccgcgtctac cccggggtga agatctgcat caaggacgtc aaggaggaca
     9961 tcaagaccga gcagaagggc atcaccttct tcctcgagaa ccagatgatc cggacgacca
    10021 agtacgagga gcccgaggac gagttcctca agcgggggcc tcccgatgcc tataaggccg
    10081 attgacctcc agtccctctt catgcagatg ggccaggtcg gccgcgagaa ggcggccgag
    10141 aaggaaggcg ccatcctcca ggcctcgatg aagggcgcgg ccgagcagaa gcgcgcggac
    10201 gaggccaagg aggcggtgcg ccgcccgacc gaccccgaag gcgggacgcc ggccgtccgc
    10261 gaccgcaagg agaggcccca ggggggccgc gagggccgcc gcgagggcga ggctccggcc
    10321 gaggaggccg aggccaagga agaggaggag gtcgtccgcg accccagcct cgggaacaag
    10381 atagacttga gcggttaggg agccatgacc agttttctcg cccttctagc tttggacatc
    10441 ctcggcctcg ccggcctctg gctctacgtc agggccaggc tgcgccgcgc cctcgagctc
    10501 gaggggctcc tcggcgagat ccgcaaggaa gtcaggggcc tcaacatcga gatcaacgag
    10561 acggccgaca ggaacatcag cctcctcgag gaccgcatgg acagcctgcg ctccctcctg
    10621 gacgaggccg acaggcgcat gggcgtcgtc aggaaggagc tctccgcccg cgcggccgag
    10681 cgggaggtct acagccacct gggaaggcct cgcccccgcg cgggtgagga agcccagggc
    10741 ggcgagggcg gctacgccgg cttcgccccg gccgcgccgc gccccgagcc gaggccggag
    10801 ccacggcccg agccgcgcgc cgccgggcgt cccgagccgc ggccggagtc acggcccgag
    10861 ccgcgcgccg ccgagggaga agacgcggag ctgggcctgc ccgaagtcgc cggggccgag
    10921 gagcccatcc gcctcgaact ctcgcgcgag ccccggcgcc cgcccgagat ccgacgggcc
    10981 agcgagagcg tcatcccccc gaggcccctg cgcgagcggg ccatcgagct ccaccgcaag
    11041 ggcttctccc ccgacatcat cgcggccagg ctcggggcca ccgtggccga gatcgagctc
    11101 ctcgtctccc tggaggaatc cctcgacgag cgccgcggat cggccgagga ttaaggcatg
    11161 ggacccgtca tcggcatcga cttcggcacg accaattccc tctgcgcctg gctggacggg
    11221 gacaggcccg cgatcatccc caacgcccgg ggcgaccgct ccacgcccag cgtcgcggcc
    11281 tccaccgcga agggcgagat cctggtcggc gagtccgcga agaaccaggc cctggtcaat
    11341 cccgacaaca cggtcgcggg cgtgaagcgc ctcctcgcct ccggcggcct cctctccatg
    11401 gggggtaaga cctggcggcc cgaggagatc gccgccctca tcctctcctc gctcaaggcc
    11461 gacgccgagc gccacctcgg gcgcgaggtc tcccaggcgg tggtcgccgc cccggccaat
    11521 ttctcggagc gggagcgccg ggccctcctg gaggcgggcc gcctggccgg cctcgaggtc
    11581 ctgcgcatcc tgaacgagcc cacggccgcc gccctggccc gggcctgggc ggccggctcg
    11641 ggctcggccg gttcggcccc gcggggcgtg gcggcgccgt ccaaggccgc ccctggcggc
    11701 ccggccagga gttcctcggc cggccaggcc gggaaggccg ccgcgggcgc cgctaccgaa
    11761 agccccggcc cgcagaccat cctcgtctac gacttcggcg gcggcacctt cgacgtcacc
    11821 gtcctcaggc aggacgggcc cgactgcgtc gtcctctcct cccgcggcga cggccgcctg
    11881 ggcggcgcgg acctggacag ggagctctac cgcagggcgg ccgaggcctt cgccgcggaa
    11941 tacggcctgg aggtcggcgc ggaccgccgc atcgcccagg cgctctcgga gcaggcggag
    12001 aaggcgaaga tcgagctctc cgagcgcctc gaggcctcca tggccatccc cttcgccctc
    12061 gcgacggggg cggacggaca gccccgcgtc gtccatccgg tcttcgccgt cagccgggag
    12121 gagttcgagg ccgtggccct gccctatgtc gagcggagcc tcgcccttgc cgagcgggcc
    12181 ctcaaggacg ccggcctcgg cgcgaaggac gtcgacctcc tcgtcctctc ggggggctcg
    12241 agccgcatcc ccctggtccg ccgccttctc aaggagcgcc tcggcctcga gccggccggc
    12301 ggcgtcgatc ccgaggagtc ggtcgccctg ggagccgccg tccaggcctc cctcctcgag
    12361 ggctcggagc ggctccgcct ccgcgacgtc gtgtcgcgga cctacggtgt ggagatcgac
    12421 ggcggccgct tcgtccccct catccgcaag aacagcccgg tgcccgcctc gcgcagccgc
    12481 gtcttcacga cggtctccga gggccaggac tcggtggaga tccacgtcct ccagggcgac
    12541 gccgagcgcg ccggggacaa cctctccctc gggcgcttcc tcctctcggg gctccgcttc
    12601 gcgaaggcgg gcgtgccccg catcaaggtg gacttcggca tcgacgagtc ggacatcctc
    12661 cacgtcgagg cggtcgacct cgacacggga gcggcccagg cgatcagcat cgccgacctg
    12721 gaccgcggcg ccggggccga gccgccggag gccctcgcgg agaaggcccg cctcctcgcg
    12781 ggcaggctcg ccgagctcaa ggacggcctc tccctcgagt cgggcctgga ggcggagctc
    12841 gaggaggccg ccctccgcgc cgagggcgcg cggcgcgacg cccccgaggg cgagctcagg
    12901 ctgctcaagg ccgagctcga gggcctggtc ggcgagctcc tcgcccggcg cggcgagctg
    12961 gccaggcgat gagccagggg gcttccgcgg agcgcgtgag cgaggagcgc ctcctcgccg
    13021 tcctcggcct cggccccggg gccgaggagg aggagatcag gacggcctat cgccgcctcg
    13081 cgcggcgctg gcatcccgac agctcccgcg accccggcac cgccggccag ttcgcccgcg
    13141 tcgcgcgggc ctacaaggtc ctctccgccc ggccgccgcg ctcgcccgcc ccgatgggcg
    13201 gcgcggccgc gaagtaccgc cgcgtgatcg aggccggcga ggatctcttc gccctcggcc
    13261 aggtcctcgc ctccgacccc gaggccggcg cccgcgaggc cgcggcccgc cgcctgggcc
    13321 tctcggggcg gacggccgcc tacgtgttcc tgaggcgggc cctctacgac cggtcggagg
    13381 aggtggccgc ggcggccgtg cgctccgtgg ccctcctggg cgcccgccag gcggagggcg
    13441 aggtggcggc cctctacagc cgagccacgc cgggcctgaa gcgcctcgtc ctcgagacgg
    13501 ccctcgcgac ccgcgagccc ctcttcaagg cggcgatccg ggcggcctcg aaggacgagg
    13561 acccggccct gcgcgtcctg gccgcccgcg cggcggcggg cgagtagccg ccgcaggcgg
    13621 gcgagtagcc gccgcaggcg ggcgcctgcc ccgccggaca ggcccggagg ccgctcccgg
    13681 ccgcggcgcg cccgcgggcg cgcgcgagcc aagtcgcccg ctcgcgcggc gccccttact
    13741 tgatcttctc cctgaacaga agccccgtgg ccgtgacggc caggccgcct tcggcgctct
    13801 ccctgatgga ggcgggcatc gcgtcgccga tggccttcat cgcgccgacc acctcgtcga
    13861 agggtatcac gctccggatg cccgcgcggc gcaggcagac ggcggtgagc gcgttggccg
    13921 ccatgaaggc gttgcgcttc gcgcagggca cctcgacgag gcccgcgacg gggtcgcagg
    13981 ccaggcccag gctgttcttg agggagaggg cggccgcgtc gaaggctccc tccgcgtcct
    14041 cgcccaggag atgggcaagg ccggccgcgg ccatcgcggc ggcggcgccg cactcggcct
    14101 ggcagccgcc ggccgcgccc gcgagcgtgg cgcgcagggc gatgaggatg ccgacggcgg
    14161 aggcgacgac gaaggcgtcc tggaagcgcg gggagtcggg gccgagctcg ggggcgcggg
    14221 cgtcgcggaa ggcccagagg acgccgggcg cgatgccgca ggagcccgcc gtcgggaaag
    14281 cgacgatccg gccgccgcag gcgttcacct cgttgatgga gagggcgtag acgagggccc
    14341 ggcggaagag ctcgtcgtcg acgggccagg cctcgccgtg tgccagcttg gccgccgcgc
    14401 cgtccgtcat cccggagaag gacctctgcg gctcgcggat gccgcggtcc agcgcctccc
    14461 tcgagacgcg gatccggcgc gcgagctcgg cgcgaagctc ctcctcggac ttgcccgagt
    14521 ggaaggagtc gcgcgcgagg aggtaggcgg gcagggagag gccggcctcg gaggcgcgat
    14581 cgaggagctc gagggcggtg gagaatttcc agttcacggc tctgtcctcc taggacctga
    14641 tcgagggcac gtagacgacc tcgcgcacgg cgggaagctc gcggatctgg gcgacgatgc
    14701 tcgcgggcac cacgccgtcc acctcgacga cgaggagggc ctcgtcgccc ttgtccttgc
    14761 ggtgggaggc gacgcccgcg atgttgaacc tgttcgcggc caggatgtgc gacacgatgg
    14821 cgatgacccc cacctcgtcg cggtgcagga ccaggatcgc gtccagggag caggccaggt
    14881 cggcctcgaa gccgccgatc gactggaggc ggatctcgcc tccgccgatg gaggcgccgc
    14941 ggatgcggat gcggcggtcg ccgctccaga gctcgaccac cgcggtgttg gggtggtagc
    15001 gctcggggcc gtcgtagacg gggacgatct ccacggccag gccctcgcgc gcggcgagct
    15061 ccagggcgta gcccaggcgc tcgtcctccg ggaggatgcc caggaggccc gcgacggccg
    15121 ccttgtcgga gccgtggccc ttgtaggtcc gcgccaggga gccgtagaag ctcaccctgg
    15181 cgccgtcgag ggggtagggg aagatcttcc tggccaggaa ggcgatccgc gccacccccg
    15241 ccgtgtgcga gctcgagggg cctatcatca cgggcccgaa gatgtcgagg agcgagacgt
    15301 tccggtcggc catgggggaa gcatagcgcg gcccgggctg gcccatcaag gctcgcggcg
    15361 ccccggcgcc taggcctccg gtccaggcgc cttctcggcc gcgccgggcg cctcgatgcc
    15421 gtactcctcg atcttctggt agagggtctt cctgccgatg cccagtatct cggcgctgcg
    15481 gctcttgttg ccgttctgcg ccgccagggt ctcgcggatg aggatcttct cggcctcggc
    15541 tagggaggag cccgcgggca cgcggatgtc gcggtcctcg ccggaggagc gcagggtcgg
    15601 cggcaggtcg tccaggccca cgaggccgcc gcgggccatg accacggcgc tctccacgca
    15661 gttcctgagc tcccgcacgt tgcccggcca gccgtaggcg tagagcgccg agcgggcctt
    15721 cgggtcgaag ccctccacgg acttgccgtt ctcgcccgcg aactccttga ggaaggccat
    15781 cgcgaggagg gggatgtcgt cgcgccgctc ccggaggggc ggcacgtgga tgttgacgac
    15841 gttgaggcgg tagtagaggt cctcgcggaa gcggccctcg gcgatctcct tcttgaggtc
    15901 gcggttcgtc gcggcgacga tgcggacgtc ggtctccacc gtctcctcgc cgcccacgcg
    15961 ctcgaacctg cgctcctgga ggacacggag gatcttgatc tggacgttct ggttgatttc
    16021 gcctatctcg tccaggaaga ggctgccctt gtccgcgagc tcgaagcggc cgcgcttgcg
    16081 ggcctgggcc cccgtgaagg agcccttctc gtggccgaag agctcgctct ccaggaggct
    16141 ctccgcgagg gcggcgcagt ggaccttcac gaaggggccg tcgcgcctgg gcgagaggtt
    16201 gtggatcgcg tcggcgacga gctccttgcc gacgcccgac tcgcccgtga cgaggacgct
    16261 cgccttcgtc ggcgcgacgc ggcgcacgag ctcgaagacc cgcttcatct cctgcgagga
    16321 gccgatgatc gagctcgacc tccgctgcgc ctcgatctcc gcctcgagct cgcggttctt
    16381 gcgggagagc tcgccccgct ccagggcccg cttcgcgagc atggagaggt ggtcaaggtt
    16441 cacgggcttc gtgaggaagt cgaaggcgcc gtagcgcatc gcctccaccg cgtcctcgat
    16501 cgtgccgtgg ccggtgagga cgatgacggg caggcccggg tagcggctcg ccagggtctt
    16561 gaggagctcg ctcccggaga gggccggcat cctgaggtcc gtgatcacga ggtcgacgag
    16621 gctctcctcg acggccttga gggcctcggc gccgtcggcc gccgtcctca cggcgtgccc
    16681 gtccagggag agggcctccg ccagcccctc gcgtatgttc ttctcgtcgt cgacgacgag
    16741 gatggtcgct cgcacctagt ccctcctcgg ggcggcgccc tccgcgtcct tccagggaag
    16801 gaggtgccgc tccttctgcg gcaccgggag ggcgatggtg aaggtcgagc cctggccggg
    16861 cctcgagcgc acgtcgatgg ccccgtcgtg ctccttgact atcttgaagg tgatggtgag
    16921 cccgaggccc gtgccggcct ccttcgtcgt gaagtagggc tcgaagatct tgggcaggta
    16981 ctcctccggg atgcccgtgc ccgagtcctc cacggcgatc agcacctccc cgtccttctc
    17041 ctccacggag aggccgagcc tgccgccgtc gggcatcgcg gccagcgcgt tcttggcgag
    17101 gttgaggagg gcctgcttca ggtagcgctt gtccaggagg acccgcggga cctcctcctc
    17161 caggctcgtc tctatctcca ccccggccgc gtcggcctcg tagcgcagga agtccgcgac
    17221 ctcccgcacc aggacgcggg ggtcggcctc gcggagctcg acgtccatcg gccgcaccgc
    17281 gaagaggaag tccacgacga tcttgttgag gcgctcgatc tcctcggtga cgacgcccag
    17341 gtgcctgtcc atggtctcgg ggacgagggg cttccccgac ttcagggcct tcttgatgag
    17401 ctggatgtgg atggagatcg agcccagggg attcttgatc tcgtgggcga cgccggcggc
    17461 cagggtcgtg agcgaggcga ggctctccgc gcgccggagc tgggcctcgc ggcggcgctt
    17521 gtccgtgatg tcctccaggt ggaggagggt gccctccagg cgcccccgcg ccacgagggt
    17581 cgtcgcgctc gcggcgacga ggcgggtgcg gccgccgggc gcgtcgagct cgaactcgcg
    17641 gtccaggacg ctctcctcgc cctccagggt gtccctgagg aactccgcga gctcgcggtc
    17701 ggcgacggag agccagaggg gccgctccga gggctcgccg ccggcgaggc cgagcattcg
    17761 ctcggccgac ttgttgtaga tcgtcgggag gtggtcgcgg tcgcagacga cgatgccgtc
    17821 gagcatcgag tccagggccg cctccagccg gccgttctcc tcggccaggg cctggaggag
    17881 gccgcggagc tgctcctcgt tcatcctggg cgccttcgcg agggcccgct cgacgaagcg
    17941 cctcatcggt cgcccatcgc gtagaggagg ctctccgcga ggagggaggg ggagaggttg
    18001 taggtcgccc gcctggacct cgcctcgcgg gccagggccg tccagccctc gacgaggagg
    18061 aggcccgccg cgtccaggcc gggctcgcgg agcagctcgc cgaagcgccc cgcgagggcc
    18121 gcgaggaagg cgtcgaagga gccttcgtag gcctcgtcct tctggccgaa gtccttcgtc
    18181 gcctcgagga ggccctggag ggcctcggcg gggccgggcg cctcgccctg ggcggagagg
    18241 cccgcggcgc aggctgccag gccgccctcc agggggccgc gagaggcgcg cagggccagg
    18301 gcggcggcga ggaagtcgcc cgccctggcg cgtgcggcct cgggcgggaa ggcgcggagg
    18361 gaggcgagat aggcggcgac gccggtcggc gcggggggcg cggagccgcc tgggccgccc
    18421 gccgcggcgc ccgcctgcgc gccacccgcg gcgcccgggg ctgcggcctc ggcggccgcg
    18481 ggcgcgccct ggccgagctt gaagacgcgg tccaggacaa ggcgcgcctc ggccgggctc
    18541 ctctccttga agacgtaggg ccgcacccgc gagaggacgg tcgcgatgag ggcgctcggc
    18601 cgcgcggaga ggaggatgaa ctccacgctc tggggcggct cctcgaggat cttgaggagg
    18661 gcgttccgcg cggcctcggc catgcggtcg gcgttctcca cgacgatggt cttcttgcgg
    18721 ccgttgggcg cgaggcgggc ccaggtctcc gcggagcgga cctggaagac gggcggggcg
    18781 tcgggcacga ggccctcgag cttgcgcgcc gcctgggcgg cagcgcgcgc ggcctccacg
    18841 gcgccggggg ccagggagcc ggcctccgcc cgctccggag ccacggcgtc cagcctctcc
    18901 tcgatctcgc ggaggagggg gagggccttg gagagcctcg actcctcgcc ctcgtagagg
    18961 gccgcgtcga agcgcttgcc gagcttgcgc gccgcgcgga cgaagaagaa ggccgaggcg
    19021 cggccggggg cgcgctcaag gaggccgagg cccgcctgga tctcctcggg gaagctccgc
    19081 ggcccgagga ggaggaggtc ggggtgggcc aggacgcggt ggcgagagca ggaggggcag
    19141 gggcagttcc agggcgcggg gccgggaggg aggccgaggg ggcccctctc gcaggagagg
    19201 acccgcgcga gctccagggc cgcgctctgc ttgcccgagg ccggagggcc cgcgaagagg
    19261 agggagggcg ggagggaggc ggcctcgagg tcggccttca gtcggcccgc cgcctcgtcc
    19321 tggccgagga ggttctcgaa cacggctcgc cctagggctt cgaggccggg gccggaggcg
    19381 cgagcttctt cagggcctcg gggaccttgg gggcggcctt ctgcggcagg gcgccgttcg
    19441 ccgcgccccc gatggccttg gcgactcccg gctccaggat ggggaggagg gccttcgcga
    19501 agaccgagtc ggagaggagg ggcttcacgt cgaagagggg ctggaccttg aggacgagga
    19561 ggatgaccgc gacgacgacg aagccctcgg ccaggccgat gacgaagccc aggaccttgt
    19621 ccgcccaggc caggttcgcg gcctcgaggc cctccttgag gagcttcccg aggaggcggg
    19681 tgaggaggaa gcctatgagg aagagggccg cgaaggcgat gacggcgggg agggggagtc
    19741 cggcgaagcg gccggccagg aaggcccccg cgggcttgta gagatagagg gcggcgccca
    19801 ggcccacggc gtaggcggcg acggagagca gctcctggac gaagccccgc acgaggcagc
    19861 ggaggccgag gagaaggagg atggcgagga ggatccagtc cagcgcgttc atgagagtgc
    19921 tcctatccgc tcgagtatga gatcggccgc cttcccggcg gcgtcgcttc ccgcgagggc
    19981 cctggccgcc tcgccggccc gcgcgaggcg ggcgggatcg gccaggaagg acctggcttc
    20041 ttcgacgagg cgggccggcg tcgcgtccgc gcccaggagg accctcgcgg cgccggccgc
    20101 ctcgaagagg cgggcgttgt ccacctggtc gcccctcgtg cccgagcctg cgagggggac
    20161 gaggatcatg ggcttgccga gggccgagca ttcccagagg gtgccggccc cggcccggcc
    20221 cacgaccagg tcggcggcgg cgacgacgtc ggccatctcg ccgtggatgt aggcgtaggg
    20281 ccggtagcgg cccttggacg cggccgcgca ctccgcgccc gcgcccgagc ccgcctgggc
    20341 ctcgcccgtc tggtggacca cgtaggctaa cttcgcgagc tcggggaggg ccgcggcgac
    20401 gagctcgttg acctgcctcg cgccctggct cccgcccagg aagaggatca ccggaacgcc
    20461 gtcgggcgcg ccgacgaagg cccggccccg gcctgcgtcg cccgagcgga tggaggcgcg
    20521 gaccgggttc ccgaccagga cggcccggcc gcggagctcg ggggagagga ggccgacggt
    20581 cttctcgtag ctcacgagga cgcgctcggc gcgccgcgcg ttgagccgcg tcgcgaggcc
    20641 gggcgtgagg tcggactcgt gggtgaagac gggtatgccc agggcggccg cggcagcgca
    20701 aggcggcacg ctcacgtagc cgcccttgga gaagaggagg acaggcctga gctcggagag
    20761 taggcgcctc gaggcgaggt agcccgcgag gacgcggaag ccgtcggccg cgttctccag
    20821 cgagaaggag cggcggagct tgcccgaggg cacggcgtag tactcgacgc cggccgcctc
    20881 gacgacgcgg cggtccgact ccttggccga gccgatccag acgatcctgc cgccgaagcg
    20941 ccccttgagc cgctcgatca cggccagtcc cgggtagatg tggcctcccg tcccgccgcc
    21001 cgtgaaggcg atgaccctgt cgtcgcccat cgcgtcaggc cttcccgccg gcgcccgcgc
    21061 cggcggcgcc gaggccgagg agcccggcgt aggcgcccag ggcgccctcg gtcccggcgg
    21121 cctcggggga gacgcccgcg aggaccttct tcgcgaggac cttgccgagc tggacgccct
    21181 cctggtcgaa gctgttgagg ttccaggcca ggccctggaa ggcgaccttg ttctcgaagt
    21241 gggcaaggag ggcgcccagg gccctcgcgt ccagcctccg gccgtggatg aggctggagg
    21301 ggcgctggcc ggggaagtgc ttgttgccgt tcggatcggc cttgcccgcg gcgaaggcga
    21361 ggatctgcgc cgcgaggttg gcgttgagct tcgcgcggct cgtcgagccc tcggaggaca
    21421 cgtcgtcgga gacctggttc tcgcggaagc cgatgaactg gagcggcacg atgtccgtgc
    21481 cctggtggag gagctggtag aaggagtgtt ggccgttcgt gcccggctcc ccgaagacga
    21541 gggggcccgt cgcgtaggcc acgggctcgc ccgagcggtt cacgcgcttg ccgttcgact
    21601 ccatgtcgag ctgctgcagg tgggcgggga agcgcgacag gggctcggcg taggggagga
    21661 cggccgtcgc gggcaggccg aggacgttcc tctcgtagag gccgatcatc gcgtcgagga
    21721 gggcgggatt cttcccgagc gccggctcca gggccaggag gtcctcctcg tgggctcccg
    21781 cgaggagctc ggcgaaggtc tcggggccca gggccaggga gaggacggcg ccgccgacgg
    21841 cgctcgtgga ggagtagcgc ccgccgatgt agtcgtccat gtagaaggag gccgcgtagg
    21901 ctgggttcct cgcgaggggg ctcgtctcgc tcgtcacggc gacgatgtgc cgggcggggt
    21961 cgaggccgag ggcctggagc cgcgcgcgca cgagggtctc gttggccagg gtctcctggg
    22021 tcgtgcccga cttggacacg aggatgaaga ggctcgccgc gggatcgatc tgggagagga
    22081 ctgagtccgc gtcgtcggga tcgacgttgg agatgaagcg cgcggccatc ttgaagcggc
    22141 cgacggcctt ggcccagcgc tccagggcca ggtagagggc gcgggggccc aggtccgagc
    22201 cgccgatgcc gacctggacg acggtcgtga agaccttgcc gctcgagccg cggacgctgc
    22261 cgtcgtgcac cgccttcgcg aaatcggcta tcttcctccg ctcgccctcg tagaaggcgc
    22321 ggtagtcgcg gccgtccttc acgacggggc ccgagagctc gcccctggcc aggtggtgga
    22381 ggaccatccg cttctcgccc gggttcatca cctcgcccga ggcgatgagg cggaacttcg
    22441 cgacgagctc ctgctcggcg gcgaggcgct cgaggagggc caggcggggc gcgtcgacgg
    22501 ggcaggccgc ccagttgtag gagaggccgc cgcccatggg gacctcggcc gccctgactc
    22561 gggcggcgtc gaggagggtc tcgagcctcg gcggctcgcg gtaggccaag agggccttgt
    22621 aggcctcgca agcgtcgaga ttcctgaatt cggccatcgc agggctcctt cgcggggact
    22681 ttggtccggc cccgacgccc gaacgccggg gcgtgtcgtt ccgaaacagg ggccgtatcc
    22741 tcaatcgcca ttctatagcc caggcaggcc ttcatcaaga gacgccgcgc gccgaaaatg
    22801 atgctatact ccgctcctgg aggcgccctt ggccgctcgc atcgagagag tcgaaaagga
    22861 attcatactc ggcgccgccg cggaggcggg caccctcgcg aggatccagg cctcgggcag
    22921 gaccgtcgac tgccgcctcg tccaggccgg caaggaatcc ctccgcttct ctcccgcgga
    22981 ggaggactcc gccctcttca aggcctggga cagggtggct gtccgcttcg acttccgcgg
    23041 ccagacggtg gccttctcag cccccgtccg caaggccgcc cgcggcctcc tcgagctcgc
    23101 ctggcccgag gcgatgtacc gcggcctctc ccgccgcttc gcccgcgtcg ccgccccgcg
    23161 cgacttctgc gccgacctcc tcctccccga cctggacctg cgcctgggct gccccgagtg
    23221 ccgcgaatac gccgaggtgg agaggcccgc ggccaaggag ggcctggacg ccgacaccct
    23281 cgcctccctc gtcgcggcct tcaaggagaa ggccgccttc cacgcctcgg agagccgggt
    23341 cgtcatgtgg aaggacggcc gcgggccggc cgacgcggcg gaggacctgg tcgcgaacct
    23401 gggccggatc ctctacatcc cctcgaccct gagcggcctc cccctggccg atccctatcc
    23461 cgagggacgg gtcatcaccc gcgcgatgct cgaggactac gagagccccg cctccctggc
    23521 cggagactcg cccctgggcg agaagctcgc cgccctgtcc cgcgaggggc tggccgccgc
    23581 cctctggtgc cccatcctct actaccgcta cgccgtgggc gtcgtctacc tcggaaacga
    23641 cggcgcctcg cggaagcctt tcgacctcag ggccgtggac ttcgcctggg agttctcccg
    23701 cctcctcgcc tggctcctca agcgccacgg ctacttcggc gccgccgagg ccgagccgca
    23761 ggccaggcgc ggcgccgtcg tcgacgcgag cccctcgggc gccctcatcg agatctcggg
    23821 ccgcgcgccg cgcctcaagc agggctcggc ggtcgagctc ctcctctccc tcggggacaa
    23881 gagctactcc tgccgcggcc gggtcgcccg gcgctgggag gacaagggga gctccttcta
    23941 cggcctcgcc ttcgagggcc tggcggagga ggccagggcc gagctggagc gcggcctcta
    24001 cggcgtcctc agccccgaag ccccgggcgg gaggtcctag cgtggccgcc ttcctctccc
    24061 tcgccctctc ggtcttgaag gactggcgcg tcatcgcggc cacggtcgtg atcctcctcg
    24121 cctggtccct tttccgctac gtgggcctcg tctaccggaa ggtgaggaac tacggcgccc
    24181 tccccaagag cgcgaggggc aggaagggcg gcaaggcccc cgctcccgct cccgccgtgg
    24241 aagaggaggg cgagggcgcc tagccgccct ccgtggcaag cctccggcgc cggccccgcc
    24301 cgagggcgct tctgataggg cgcctcaaag agggccgcct ctccgagggc cgcccgcgcc
    24361 gagggccgcc cgcgccgcgc tcgcgcgcgg accccgcccg tctgcgctat gattggggca
    24421 tggccaacaa gacgcccatg aacggctacg gctccctcgc gaagcggatc aatctcttcc
    24481 cccaaggcgc gccccccggc gagctcctgt tccggatcct ctccgtcctt ttcagcgaga
    24541 aggaggcggc cctcctggcc ctcctcccct tgcggccctt cacggccgcg gcggcggcgc
    24601 ggctgtggaa gctccccgag gccggggcga cggcccttct cgaggacctg gcttcgcggg
    24661 ggatgatcct ggacatggag acggagggcg ggcggatctt cacgatgccg ccgccgatgg
    24721 cgggcttctt cgaattctcc atgatgcgcg tcggcggccg ctatgaccag cgcttcctcg
    24781 ccgagctcta ccaccagtac ctcaacgtgg aggaggactt cgtccgggag ctcttcgcgg
    24841 gcggggagac ccagctgggc cgggtcttcg tgggcgagaa ggccttggcg gaacggcctg
    24901 cggcggccgg cgccgtcagc gtcctcgacc acgaacgcgc gagcgaggtc gcgaggacgg
    24961 ccacccatcg cggggtcggt acctgctatt gccggcacaa gatgtcccgc ctcgggaagg
    25021 cctgcggcgc gcccatggag atctgcctca ccttcaacgg cgtggccgat tccctggttc
    25081 ggcacggcgt cgcccgcggc atcgacgagg cggagtgcct cgacctcctc tccgaggcgg
    25141 aggagcgggg cctcgtgcag ttcggcgaga acgtccgcga gtccgtgtcc ttcatctgca
    25201 actgctgcgg ctgctgctgc gaggccctcc tcgcggccaa gcgcttcgcc ctcctcaagc
    25261 ccgtcgccac gacagcctac cttcccgcgg tcgacctggc ggcctgctcg ggctgcggga
    25321 ggtgcgtgga ggcctgcccc gtggagtcga tggccctcgt ctccgcctcg gacccgcgct
    25381 cgccgcggcg gaagaaggcc cgcgtcgacg agtccacctg cctgggctgc ggcgtctgcg
    25441 tccgcgcctg ccccgggggc gccttgagcc tcgccttccg gggcgcgagg ctcgtcaccc
    25501 ccgtcaattc cgcgcatcgc gccgtcctca tggccataga gaggggcaag ctccagaacc
    25561 tcgtattcga caaccaggcc ctcgcgagcc accgggccat ggcggctgtc ctcggggcca
    25621 tcctcaggct tccccccgcg aagcggctca tggcgagcag gcagatgaag tccgtctacc
    25681 tggagcgcct cctctcgagg aaggagttcg tcaggaggga gggctaggag gccggcacgc
    25741 gacgcgagga cgggccgacg ccctggcgcg gcccccgcct cggccccgcg agcttcccct
    25801 tccaggggcc tttcccgacg aggcgaataa ggccgagctc cgccgcggcg cgcagggccg
    25861 aggccgcgtc ctccctcctc cagccttcca tcgagccgga gcccgcggcc aggggcggct
    25921 cgaggccgcg cgtcccgagg aggaggtcca gggcctcctt ctcggagaag cggcgcgagt
    25981 tcagcctggc gaaggcggcg agctccgctc cgccctcgaa gccctcctcc gcggatccct
    26041 cgcagcggtc gcagcccgag cagggtcccc gcggctcggc cccgagaagg cggagcaggg
    26101 actcgcggcg gcagccgccc tctcccgccg cgtaggcgag gagggccgcc cgcctcgccc
    26161 tccttcccgg atcggcctcc cgcccgagcc tggctccgtc gtccctgccc gcgacgagga
    26221 cggcgcgcga ggggaggccg tccctcccgg cgcggccagc ctcctgaaga taggcctcga
    26281 ccgacgcggg cgcctcgtag tggacgaccg agcggatgtc gcgcttgtcg acgcccatgc
    26341 cgtaggccga agtagcgcag aggatgcccg tcgcgctgcc gtagaaccag gcctcgacct
    26401 cgcgcttctc ctcccgctcg aggccggcgt ggtagaagcg gatgtcttcg cttccgagcc
    26461 tggctcgcag gagttcggcc aggatctccg ccccggctcg ggaggagacg aagacgatga
    26521 ggggcttggg aaggccgcgg cagaggcgct ccaggctcct gttccgcgag agggtcctcg
    26581 ccacggagta ggagatgttc ggcctgtcgg ggtcgcctga gaccaggcgg tatccctcgc
    26641 cgcggaagag gagccgctcc agggcctcca cgacggaggg gctcgcggtc gcggtgaagg
    26701 ccgacagggc ccggggcgcg agccgctcgg cgatctcgcc gagctcgagg taggccggcc
    26761 tgaaggtctc gccccactcg gagacgcagt gcgcctcgtc cacggccagg tggctcggct
    26821 tcagggaggc gaggaagtcc aagaggccgc tcgacccgag gctctcgggg ttcgtgatcg
    26881 cgagcttggc ccggccgctt tcgaggtcgg ccatcgcggc gcgccgctcg ctctcttcct
    26941 ggccgccgcg gaagagggcg taggggatgc cgagccgcgc aaggctcctc cgctggtcct
    27001 ccatgagggc caggagggga tagacgacga tcgtggggcg ggggaggacg agggcgggga
    27061 gctggaagca gagcgacttg cccgctcccg tcgggaggag gacgagctgg cgccccgtct
    27121 ccccttcctc ggattccgcc gcgtccagga cgttcgcgac ggccaggcgc tggagggggt
    27181 agagccaagg gacgccgaag cgctcgcggg ccagcctcgc gacgggatcc tcgccttcct
    27241 cctccatgaa gagcatacgc gcgcggcctg gcccggcgct tcaaggcgag ggggccgcgg
    27301 cccggagctg cggcggctgg ggccggcccg ccgcgcctgg acgggcgctc tcgccaggcg
    27361 cggcgcggct tccctcgccc cgcccgggcg cgctagaatc gaggcgtggt agacgcgcat
    27421 cttgaggggg aggactggga cgagccccac gaggccatgg ccttcgaatc gtcctacggc
    27481 ggcgtcatcg agacgctcaa ggagctcgta gcgggcctgc gcgcggcgga ggccggggaa
    27541 gggccgcgcc cgccccacga ggagctcgcg gcccgctcgc gggccctcct agtccgcgcg
    27601 ccggccctcg tcaacgtcat gctcaactac aagatctgcg ccgagcacgg ccttcccctc
    27661 catcccaccg tctactacga gctcgcggag gcgcggcgct accgcctggc ctacccgata
    27721 gccgagatcg agcgggcgaa ccgcctcttc cgggactcca tcgacctggc ccgcgcggcc
    27781 tacggcctgg ccggggactt cgaggcccgc gccgaggcct tccgctcccg cctcccgccg
    27841 gaggtccagg acttcgtcta cacgggcacc agggacaagt acacctggcg ggcctcggag
    27901 cccgcgaagg tccgcgccct cgcgcgcgtc gtccgcgagg gcttcaatcc ggggctcgtc
    27961 gtcgcggcgg cccacggctc gatcatgccg ggcctcctcc tcgcggagta cctggatcgg
    28021 ccgctctact tcctgcgctt ctccatgttc aagcggaagg acgaggcgcc catcctctcg
    28081 atcgccgacg aggcctggct ggccgagcgg tcggaggagc gcgccctcct ctttgacgag
    28141 gacgtcgcga agggcacgac ccttgcgatc ttcgcggagc gcctgaagca tctcttccgc
    28201 gagtccagga cggcctgcgt catcaggcac gcgggctcgg gcctgcggcc cgacttcgcc
    28261 gcgcgcacct ggtgggatta ggcggcgccg gagcgtagcc ggcgcccgcg gcaagttatg
    28321 cgcgcgcggg cgcgaaggag gggggcggga cccggggtcc cgcccgcgtc tctttagacg
    28381 aacatgtcct tgttgaggaa gccgaccacg gcgcccgcga ccgaggcgac gatcgtcacg
    28441 acgctcagga gggcgaagac gaccaccagg gcggaggcgg cgccgacggg caggggcggg
    28501 tacttggccg ccagctccgc cgcggcctgg cccaggttcc cgaaggcggc cggcacgaag
    28561 aagcgcgagg ccgtggtgga ggcgaggagg gcgacgggga agttcatcgc gctcagggtg
    28621 aagcccgaca cggagcgggt cttgaggccc cgcaggccga ggaaggtggg cacgaggaag
    28681 atgagggcca gcatcgggcc gaagaactgg ggggagagga cgaagaagcc gatcgtcgcc
    28741 gcgaagaagc agcggacgta ccacgtgtag gcgcccaagc gtccggagag gtcgtaccag
    28801 gccgcgttcg cgtggcggaa gaatttctcg gcctgttcgg ccttgagggg ggaggcggag
    28861 aggagcttcc tgccccgctc cacgaggccg gcgccgacgc cgcccgtctc gtcgatctcg
    28921 cccagggcct cgtacctggc ccgcgtcttg gcgagccggg cgagggccct gtctttctcc
    28981 ctccccgaga ggaggtcctt cttgatgccc ttgtccaggg tgtcgaacag ctcgaagagt
    29041 gccatggtcc ttcctagaga aagcgacgcg gccggcgcgc agggcgccga ccgcgtctca
    29101 agatggttgt tctacctgct cacttcgagt gcttgagaag cgagcggaga ccgacgacgc
    29161 tggcgccgat gatgaggatc caggcgccga gcatgaagat gagcgcggaa ctattcatgg
    29221 cctactcctt caccacgagg acggtgttct tggcgatctc ctccgggtac ttcttgcgga
    29281 tggagaggtt cgtctggatg acgccgagga ccaggaccgc gaggaccacg atctggccgg
    29341 ccatggtcca gggcgaggcg ctcatgatgt tcttgaggct gttgcccagg accgcgccgc
    29401 cgcccgcgag gtagaccacg tagccgacga ggaagccgat gatgaggacg ggcgcggcga
    29461 cctggatgaa gagggtgtag taccacttcg ggagcttcca gtaggagccc ttgttgatct
    29521 ggtccagtcc cttcttgccg aggatccagc ccgtcacgac gatctcgatg aggccgagga
    29581 cgacgaggaa gacgttgccg acccagaagt cgacggcgtc caggtaggcg gtgctgcccc
    29641 tgatgagggt gggctcgatg ccgacgggga ggccgactat gatgtagagg gcgaagacga
    29701 tgagggcacc cgtggagcgc ttgatgccca tgtcctcctc gaggagggcc acgaggtagt
    29761 tgtacatggc gatggcgcta gtgaagcccg cgaagaagag gaggaggaac cagaagccgc
    29821 cgaagaactg gccgccggcc atgttcctga agacgttcgg gagggagatg aaggagagtc
    29881 ccacggactg gccgatgccg tcggggccga ggaaggcgaa ggcgatgggg atggcgatcg
    29941 tgccgcccag gatgacctcg gcgaattcgt tgagggcgat cgtcgtgaag ccggagagga
    30001 cgatgtcgtc gtcggccttc aggtaggagg cgtagttgga gatgatgccc atgcccaggg
    30061 agagggtgaa gaagatctgt cccgtcgccg caagggcgga gccgaagttc aggctcttga
    30121 agtccggggt ccagacgaac tcgagtccct tgatcggcga ccagtcggga ttgacggggc
    30181 tgccgagggt gagggccttg acgatgagga tgacgccgaa gacgtagatg agcggcatca
    30241 tgacccgcgc ccaggcctcg atgcccttct ggatgccgcg catgacggcg aagccgaggg
    30301 ccgcgagggc gatgacccag aagatcatgt tgacggggtt cccgatgtag ttcaggaaca
    30361 cgtccaccgt ggagacggcc gcgtccttgt agcctcccgt aagggagagc caggtgtagc
    30421 cgagggtcca gccgatgatc tggttgtagt aggagttgac caggatgacg accgcgaagg
    30481 cgaggccgcc ggcgagggcg ccgacgccca gggccgactt gggcttcagc ttgtcgcggg
    30541 cctggaggta gaccatgggg cccagggtcc cgtggccgta cttgccgccg gtcttgccca
    30601 ggttccactc gacgaacatg aggggaatcc cgatgaagat gagggcgata aaatacggca
    30661 ccatgaaggc gccgccgccg ttcttcgccg cctggtaggg gaaacgccag aagtttccga
    30721 gtcctacggc gttgccggcc atggcgagaa tgagtcccag cttggaaccc cagttttcac
    30781 gcttggtttc cgacattttg cctccttgtc gaaaaggtac ggtattcctc ccaggccgcg
    30841 taaaactacg cggaagggca ggatgggcga ttatacccgc tccgtggagc cgaaacgggg
    30901 aaaaaactga agtatcattg gctagcccga ctataggact taaccgatat gtagcggtcg
    30961 ggagaatcgt gatggtttga ctaagattta tgggaaatta aagattttag accgggaaca
    31021 gatgggtgat attcctaatt tatgcatacg aggaattcct gaaaatcccg gcattttgct
    31081 gtcgccgcat cgatgtcgca ttttgcgaga attagggctt gcgtcgcgga ggctcggatt
    31141 gtatatttat tccagagttc gtcatgactc tacgctgtgc gtaagctccg gggttttaac
    31201 ctcctttttc cccggagctt ttttttcccc cccccttcgt tccgccttcc cctcccgcgc
    31261 cgaccatacc gcgccagtat ccaaagttgc ctctagatta gcaaaagctt atgttgactt
    31321 ttttatcgac ttcgttgtag actgaatatc taatcggagt gcaaatggcc aagatcaaag
    31381 tctcctacgc cccttccgtg cgccgccttc cctcctatct ccatataatc aggcagtccc
    31441 agcgcgcggg ggactactac atctccggaa ccgtcatcgc ccaggagctc aacctcgagc
    31501 ccatccaggt caggaaggac ctcgcgatca cgggcatcat cggcaagccc aagaaaggct
    31561 acccggtgga ggccctggtc gccgcgatcg agcactacct gggctgggac cagtcgcacg
    31621 acgctgtcct ggtcggagcc ggcaacctcg gctcggccct cctcggctac caggagttcc
    31681 agctgcacgg cctccacttc gtcgcggcct tcgacacgga tcccgcgaag ataggccagc
    31741 acatccacgg cgtgcccatc ctctccatgg acaccatcga gcaaggcatc aagagcctcg
    31801 gggcggagat cgccgtcctg gccgtgccct cgcccaacgc ccaggcgacc ggcgagaccc
    31861 tggtccgcgc cggtatcacc tcgatctgga acttcacgaa caccaagctc aaggtgcccg
    31921 acgagatcat cgtccagcgc gaggacctct cctcgggcta cgccctcctt tccgtgatga
    31981 tgcagaaccg caagggccgc ctcgtctgac ggacaaggac gcgaggcagg ccgagtactt
    32041 cgccaaccgc ctggccaaga acgagaagaa cctcaggcgc tgggcgaggc gcgagggggc
    32101 gggcgccgtc cgcctctacg accgcgacat ccccgaggtg cccct
//
LOCUS       DAOOSJ010000004        31765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1027_length_31765_cov_6.109398, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000004 DAOOSJ010000000
VERSION     DAOOSJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 31765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31765
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1027_length_31765_cov_6.109398"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLG14_00485"
     CDS             <1..912
                     /locus_tag="PLG14_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOX47631.1"
                     /translation="AAAAAYDALAEEGKRLSAAAEPQAASGAAGGSAMAARGGGSSST
                     PPAGPEAGTAAAKRRLPVSYRPPLEPPKGGAKGRLVLVIDDVGYSLSELEPFLALPFP
                     LSLAVLPGLPHSEEAARRIRAAGKELLLHQPMEASGGQDPGPRAIYLSTPPEEAARLL
                     EENLDSLPGAKGVNNHMGSAVTREERLMEPVLEVVKRRGIYYLDSLTASGTATSGVAS
                     RERIRYWERDVFLDNSPDRVSIVRYIEEGKKKAEKGGPAVMIGHVWSADLAQTLADLY
                     PELVAEGFSFSTISKIMIEEADENTGD"
     gene            893..1906
                     /gene="tsaD"
                     /locus_tag="PLG14_00490"
     CDS             893..1906
                     /gene="tsaD"
                     /locus_tag="PLG14_00490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOX47632.1"
                     /translation="MRILGIESSCDECAAAVVEDGRRVLSDVVATQIPFHARFQGVVP
                     EIASRKHTEWVYGVVEKALSDAGTAPAELDGIAVTAQPGLVGSLLVGIQFAKGMAYSL
                     GKPLVGVNHILAHLYAPQLESPVEYPFLGLLVSGGHTIICKAEDFDRIEVLGTTIDDA
                     AGEAFDKVSKYYGFGYPGGAVIDALAEKGDDTAFAFPKPSLHKGEHRYDVSYSGLKTA
                     VIHQLEQYRREGAPSNAENIAASFRKAAVDILLSRLFRAVEDTGLKTVVAGGGVAANA
                     YLRRALAARSDLDARFPPLKLCGDNGAMIAGLGCRLLARGDRSGFDLNAAARVPLFRK
                     PQR"
     gene            1997..2617
                     /locus_tag="PLG14_00495"
     CDS             1997..2617
                     /locus_tag="PLG14_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47633.1"
                     /translation="MGKTPVIAAVLALAAILKAAASPAPVQAPPYYLEFLAGPTLGLD
                     RVLRGGSAALGAGFAPPCLGGLGLGARAEAAYDAGLRAFIGRAELLLALGPGLALGLG
                     AELPLGELSLREPRSGCGIGLEPAALPSRFSLEALVAEGKARGGGKPRAGLSAELAWS
                     AYRALGVEPEGEGALPSLAEALSAESGFAAGLSCALRLRLAWGPAF"
     gene            2846..>3915
                     /locus_tag="PLG14_00500"
     CDS             2846..>3915
                     /locus_tag="PLG14_00500"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOX47634.1"
                     /translation="MSDSFSSSLELAPALRSAAAVACRDSLKLRPGEKVLIVTNPLRD
                     VLEISAALFDAAREAGASPSLLVQPVKAQMDYAEESVIAAFEAQPDVVMSISAEKLGK
                     DREGIRTPYEWDGTSYDHIFHYQLRGAKTIRAFWSPNVTREMFARTVPLDYAELGERC
                     RRVAAVLAGAEEVRVTNPNGTDLRLGLRGRKPLRDDGDYSRPGSGGNLPAGEVFVSPE
                     LGTARGLVVYDGSIADQAGDILIREPIRCSVEGGFVVDIAGGSEAAALRRAVDTAAAN
                     ARRMAADGLLSPAKAEAYARNARSVGELGIGLNPLASVAGNMLEDEKAFRTCHFAIGS
                     NYDEDAPALIHLDGLVSRPTIVA"
     assembly_gap    3916..4015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4534..5694
                     /gene="rsgA"
                     /locus_tag="PLG14_00505"
     CDS             4534..5694
                     /gene="rsgA"
                     /locus_tag="PLG14_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOX47635.1"
                     /translation="MFHIHVEFDDNEGAPRRDALRDFGFDESFLRDYLAEAELGDAPG
                     RVVEVQRGRCTAVTRGRDGSLSEVEAVVSGRFLHEAESAAELPAVGDWVVLRPSEGGA
                     AQLRSVLPRRSAFARRAAGVAEDRAEAQVLAANVDTAFIVAAAGRDWNPRRVERYVTL
                     AYEAGVEPVLVVTKADLAEDAEGLAAEAAALSPGLASALVCAPEGRGLECLKAWLRPG
                     KTVVLLGSSGAGKSTLLNALAGRELADTGSVRADDERGRHTTTHRELYRLDSGALVID
                     NPGLREVGIYAGEESLQAAFADIEALAAECRFRDCAHGSEPGCAVRAALESGELDKGR
                     YSGWLKLGREIAHMNAQPGSAEQRARDARWKAINKSMRGYTKERRSIQGRAR"
     gene            complement(5854..6945)
                     /locus_tag="PLG14_00510"
     CDS             complement(5854..6945)
                     /locus_tag="PLG14_00510"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOX47636.1"
                     /translation="MPEKVAVVGAGAWGTAIAKVLAELGHEVCLWCREPALAEAVRTR
                     RENAAYLPGVLLPEGLGAETDLLAAAEGRDVIVLAVPSPYLMGVVRKLLPAPSLIEGE
                     SLVVVVTKGFIPGPRGPRLILDALEERLPGRYRDQLVYLSGPSHAEEVSRGRVTGLAA
                     ASASGRNAIRTRELFRGSRVLVYPSLDAVGVQACAATKNVVAIAFGMLDAFASGAASG
                     AAAGAAAGIFGDNTESLLLAAGLNEMQLLGRALGASHPETFTSLAGVGDLDVTCRSVH
                     GRNRRFGRDIVEKGILSRFSSIEDLVASIGEVGYLPEGAVAAGQVRALAEEKRLDLPI
                     MDGVYRILNRELAALEFLESYLGSLGAPL"
     gene            complement(6951..8843)
                     /locus_tag="PLG14_00515"
     CDS             complement(6951..8843)
                     /locus_tag="PLG14_00515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOX47637.1"
                     /translation="MAGSEKLPRWDLAPVYPGFGSPEHAAAKERLTRLSAGLLAHLGA
                     APAAPAAPSGGAASGPAERAAFADWLAKALELEGEAGSLYETLSSYAYASYSTSTRDP
                     KAMAELNASEELGLPLKRASVLLRNALAARRSEVESLAAGPGADPRIAPLAFHLREEL
                     FWQSKQMSPELEDLAEDLSRSGGEAWSRLQETLFSNASVLWDAKTGERKTVVELRNLA
                     YDADRAVREKAYRLELEALKAVEIPMAAALNGVKGETLTLNRRRGWEGAVDKSIEQSR
                     MTRATLDALIGAMEESLPTWRRYLKAKARALGLGSLAFHDLFAPVAPAAKDDPAKDGA
                     AKPSGEPAYTFDAALDYIAEKFSAFDPAMGDFARKARAERWIDAEPREGKVGGAYCID
                     FPDAKAARVLCNFDGTFSSLTTVAHELGHAWHHECVKSKPYALTQYPMTLAETASIFA
                     ETVVWNEAARAAPASERLGLLETHLQDGCQVICDILSRYYFESAVFERRGKAELPPEE
                     LCSLMLEAQRRTYGEGLDGERLHPYMWAAKGHYYRPDLSFYNFPYAFGLLFGLGLYAR
                     GAKEGPAFASAYRALLAGTGSATAVEVTRSAGFDIESLGFWRSGLSVFFAEAEEYERL
                     VAEAGR"
     gene            complement(8830..9279)
                     /gene="ndk"
                     /locus_tag="PLG14_00520"
     CDS             complement(8830..9279)
                     /gene="ndk"
                     /locus_tag="PLG14_00520"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOX47638.1"
                     /translation="MALERTFTMLKPGVLSRRIAGEIISRFERKGFDIVAAKLVRLPR
                     PLAEAHYAEHRDKPFFGELVDYITSGPVVAMVLEGDGAVSMLRKLCGSTKAEEAAPGT
                     IRGDYAMHTGLNIIHASDSAASAGREIGLFFRPEEIIDWKDGNDGWI"
     gene            9471..10511
                     /locus_tag="PLG14_00525"
     CDS             9471..10511
                     /locus_tag="PLG14_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47639.1"
                     /translation="MNSEFHYYAVHFLCLRSGFAPEEARRIARSSQYVDDASVAYVVE
                     DGRAPYRCLVTQNYVFWDEATLREVYLPFHFLPGDPERARRERRDGRAHELAASPDSP
                     FSKELLVEALRSGDPYRLGIALHAYADGWAHQHFSGRIEEANALDPSSPLPPAGHLQA
                     LRSPDEALGRWHDPRLFPEFAEVNNRRRFLEAAKKIYRYLRVSRRLPFDDEELVLGEL
                     EGIWARKAGDPGQAAGAAPSAAQARTAARARLDEFVIQFDMEPYDKREWLREAGIAVP
                     GGDEEPFVGYDKLRWLGSRLKSGGAAPELRVGSGGRFEDSELRRWDEAARAHLRAAKS
                     LMKAAGIAEAQG"
     gene            10508..11479
                     /locus_tag="PLG14_00530"
     CDS             10508..11479
                     /locus_tag="PLG14_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47640.1"
                     /translation="MRPLKLARAAPASVASAAPAALAAARAAARASAAALAAVILLGG
                     CAARIRYETPVDPLAVLAGDAFLYARIRGPSLALLAPALLPEEEARAIAPLIARSREL
                     GLALTPPGEPGERPGLEGLLVGDYPFRSASFALSGDGAWKREGPGYANAAAGVRVALP
                     GPTVVLAASGPIEPLVARLKAPGPEPIPARLAPLAGREVVLYAPLPFDRLAESLLGER
                     LDIPARGLILAAERAGAVPGAPEGAGSEAAYDLALAFLMEDEDSARIYKGAARLAWYA
                     LVRSLFPEEAEAILSRRIEAEGDLVAAAGIRVRASSLAGALSRLGGR"
     gene            complement(11606..13270)
                     /locus_tag="PLG14_00535"
     CDS             complement(11606..13270)
                     /locus_tag="PLG14_00535"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX47641.1"
                     /translation="MKQSRRIAPFILLSFLALCAALPAGGEAQAAASSSAAPSAQAAP
                     APTATPAARIEPAGAVDAPAASTAPAAASAVPSGRWDGWLDFGQGKAPFSLRLLPGGG
                     GLLDLPGQRLYGYPLASIRVEGASVAFLVGGGEGALLFTGSLAAAAQAAAPARSTAPA
                     PEAAAAPAYGAAAAAPAGSEGTRAAGDAVAEAAPDGRPRISGRLSGQGAEGLFELALS
                     PPEAAYGAALEVAVRGGVLRGSLLLPEGEGPFPLVVLVAGAGETDRDGNNYNVPGRND
                     GLRALALALRGEGVASYRYDKRGAGESYALVASEAELSFDDYVADCAAVAAALGADGR
                     FSRLVLAGHTEGGLVAAAAAARLGKSVPGLALLCPSGKSAAESVEEALAEAPEELRPE
                     AEAIMAALRRGETYPEPSPYFADFFRPSFQPYLASWFRYDLPKELSIYAGQLLLVQGN
                     RDFQVSLAEFSVLAALEPDAAAVVLPGMNHVLKEVGPDLEENYRSFGDPGFPLAEGLG
                     ALLAAFARGEAPDEGLLRVDGGVLPSLPGAGEAGAEGQGAGEEGGE"
     gene            complement(13422..15821)
                     /gene="pbpC"
                     /locus_tag="PLG14_00540"
     CDS             complement(13422..15821)
                     /gene="pbpC"
                     /locus_tag="PLG14_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOX47642.1"
                     /translation="MSVAALRGGAARSSPGRLLATLPPAAAALALAALFLWGPPRPLA
                     PGSLSTALFDREGGLLGASVSAEGYWQFPPGVRLPDKFVRALLAYEDRRFLSHPGFDA
                     RSILRAALQNARAGRVVSGGSTLSMQAARITLPPGERRMGRKLAELALALRYELLLGK
                     RGILELYAARAPFGGNVVGLEAASFRYFGRPPEGLSWAEAAALAVLPNAPSAAHPGKN
                     RGLLLEKRNRLLRLLAARGELEEEDLGLALAEPLPPEPFAIPSLAPHLLDRARAEAAA
                     AGLAGARVESTIDPGVQERAAALVERRAARLAASGVHGAACVVARVDTGEVLAYVGNA
                     GLGADEDRGQRVDVVRSPRSSGSLLKPFLYAAALDSGEITPKALLADIPTRVGSYGPE
                     NNTGSYSGAVPAEEALARSLNVPFVRLLRSFGVERFRRLLLGTGMTTLTRPAEDYGLT
                     LILGGAEITLWEAAGRYAALARTARGLEAPGGGQYFDLAYTREAASRRAARPDPFSPG
                     AAWLTLEALLEVGRPEDEASWREFASARKVAWKTGTSFGFRDAWAVGLTSEYVVAVWA
                     GNASGEGRPELRGSTAAAPLLFDLVAALPRSPWIPRPSSALEYATLCADSGLPAGPDC
                     ARTERVLLPAGAAAKADSACPYCRLVQLSADGAYRVTAEGERLDLIRTERRFVLPPAM
                     EWYYRRAHLDYRPLPPWKPGTPAPAGGGGGLALLEPGEGASLYVPIELSGRPGAAVFS
                     AVHRDSRALVFWHLDGEYLGSTRGTHRMEARPGPGWHLLSLVDEEGREAERRFEVLNE
                     R"
     gene            complement(15818..21571)
                     /locus_tag="PLG14_00545"
     CDS             complement(15818..21571)
                     /locus_tag="PLG14_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOX47643.1"
                     /translation="MRNFRSFAAILAITVLAASCSGGLGKAKAPDPARVEAHTAGLVS
                     RESPVRVVLLGASGVEGASPAANPFRLEPPVKGRAVWEDERTLAFKPDRPLEPGRLYK
                     VRFDLGLLVGRAGGASGAVGAAGAEAGTGAKAAKGGTEDYFSFSFRTVEQRLEVAFEP
                     LRAGADGSLVLEGSLSLAEGAGAAEVERVLAAAGPALPGGLQVAWSHDSARLHRFAVK
                     GIARGPEPAELKVSWKGRALGAPSGGSRDFRVPPAGSFELLGARAAEGGRVELSFSEP
                     LDRRQDFRGLVSASGVESLRFDPEGGRLSLYATAWPASARIKVEPGLRAQGGATIAVA
                     AAAEVSFAWEKPELRFLSKSAILPTSQGLTLPVETMNLSALIIEAVRVQGDNMLQFLQ
                     VNDLDSSCDVRRVGEVVWRKTVDLGWKEDWKNRWVRQGLDLSPLLAAHKDGMFQIRIT
                     FRKDHIRYVCPTEHDFRDLEFPGDEIVDRGDDEYSLWDYVQEWGDDGYGQFYKYKNDP
                     CHPAYYLPAYDHDITQKRNVLVSDLGATARLETDGAWTLAASDLRTAAPVQGAAVSLY
                     NFQRRLLSEGRTGADGMVRLLPGKEPSFAVVALGSQRSYLKLDPGSRLAVSHFDVGGE
                     KADDGVKGFIYGERGVWRPGDPIHLTFVLYDRSGSVPAGLPVLYELEDPRGRVVRSGT
                     STGAVNGFYSIETSTNSDSPTGPYLARVRVGGRSFTKAIRVETVMPNRLKVKLELGGA
                     PYLAADSAPMRLESAWLTGAPAGALRADVSATFQAAGTSFTSFKDYDFDDPTREVSGE
                     RAVLFEGELGPDGKTSFPVDLSPEGLAPGRLRATLLTRVFEPSGVFSSETAQFDYHPY
                     GRYAGLKLPKGDQARGMLLTDQEHRVELALVDRDGKPVPSGTVEVSLYQLEWRWWWEK
                     GDESLAETANSIYERPLKRERVRVEGGRASFGFKIAYPNWGRFLIRVEDVSEPRSGRS
                     AKPLHSAGKVFYVDWPGYAGKGRGEGGGAASMLSLEAGKEKYSVGEKVQVSFPSNAEG
                     RALVAIERAGRLVRQEWVRAKGDRTVYEFQATPDMAPNVYIHVSFLQPHLQTANDLPI
                     RLYGVVPVMVEDPATRLSPLVLAPSEIEPNSEVSFSVREASGRPMTCTVAVVDEGLLG
                     ITRFRAPNPWDEFYKKEASALASYDLYQYVAGAFSGKLETLLAVGGGDEGLGGGQRKV
                     SRFPPVVQFFPPFEVKAGETVRKSFRMGPYVGAVRFMVVAGSGSIGSPSGAVGAAKAG
                     PAAPAAGAAYGAFEKEVPVRSKLMAQLTAPRLLSPGEEAAVPATVFSFLGKKRVRVSL
                     AVSGAAALAGPGEQYLDYAADGDLAVPFRVKAGAKPGAARLVLRAEAEGFSAEQAIDL
                     EVRPLGIPVTAVVEAGVEAGKEWSAEIAYPGAPGTNKASLELSRLPTLNLGERLAWLI
                     QYPHGCSEQTTSKAFPQLYLPESVALPPERLEEVRRNVSLAIAKLGDYQTPRGGFTFW
                     PGEGADQDWLSAYVAHFLVAARRAGYEVRDGVLDPALAYLAQGARLWNSRAEWSKSVQ
                     AYRLYVLALAGKPEPASMNRFREYKPFPAAAHYRLAAAYALSGQREAADSLLRAAPAS
                     FEVYEGLDEYTYGSPFRERAAVLEALNALGDLERGLPVYRQIALELNEPGRWLSTQEI
                     GAALSAGLPYASRASKGEAPELLVTAGDGTQRRVRLDRASARIDLEPGEAVSGAYSVK
                     NLSKTPVFAKVVARGVPAAGQESAVSNGLSISVGYFDMDENPIDPNALPLGQDLVIEA
                     VVRNRTTLKLRNLALTQVLPAGWEIANYRVGEELPRPREEGEEEERRKEAPPLYEYQD
                     IRDDRVLTYFTLEGRESKTFRIYANKTYDGSFYLPSSSVESMYDARYQAVEPGRWLSE
                     PPRSGIDAKRRERNMRP"
     gene            complement(21722..22366)
                     /locus_tag="PLG14_00550"
     CDS             complement(21722..22366)
                     /locus_tag="PLG14_00550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOX47644.1"
                     /translation="MKLIFLGPPGAGKGTLADLAVQERKLPHISTGDLFRAAVKDGTE
                     LGLKVKGILASGGLVPDDLTIALVRERLAAPDAKAGWILDGFPRTIPQAEALEAFAPA
                     DKVVNFEVDDAVVVARLSTRRVCRACGKIYNTRTMPPKKEGACDACGGEVYTRDDDKE
                     ASIRTRLENYRKQTAPLIAWYEAKGKLLTIDGVGEPKLVLTRFDAALGSASGRA"
     gene            complement(22547..23572)
                     /locus_tag="PLG14_00555"
     CDS             complement(22547..23572)
                     /locus_tag="PLG14_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47645.1"
                     /translation="MAGRREACEAEGRRFATREAGRVSCRLRRIFAGLDARPYLGDDA
                     RREFRFYAELFEEALLRIDRKVAEGRLKPLAMPRRPEAFRPPSPLARVGVFIGSFDPF
                     QMTHLAAALRFLASDRCGSDIVLVAPEGGENPSKPRRTDYGFRYELLRLQLAGILSPF
                     VVPLDLGEGEDTIGVVERLLGRCRPGRLELTHLLGSDALPLALRYLPEDLAAWSAAAA
                     RAGVELDLGAFVILRGAEGEEAVPGGAAGLAAAEEAYRSRGLRFAVDERRLSTPSSSD
                     FRARGALTIVLPTDAVLGRLELLFRYAMNRPWMEGVPAAKDGTAADAGTAAESRRAEG
                     PPPEYEI"
     assembly_gap    23613..23712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23713..24658)
                     /locus_tag="PLG14_00560"
     CDS             complement(<23713..24658)
                     /locus_tag="PLG14_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47646.1"
                     /translation="MESPKPDSKGGPVPGRLVSEAAGRIERRLRRVFAGLEAVGYLGE
                     AERAEYRYYEGLIGEALGRIDRKILSGGLRPLDLRDFPQCVRYPGYSARVAVFAGSFD
                     PFQMTHLATALRFLASEDCESDAVFVVPEGASNPLKPRKTEYRFRYEIARLQLEGIFS
                     PLVVPLDLGEGADTIGIVRRLIALHPGASLRLTHLLGSDSLPTAIRLLPEDLAAWRSE
                     AERCRVDLELGLFVVPRGEDEPPEEAARAARALGVRFAADTSRVGAPSSTDFRSRGAL
                     TIAFPSPALLSRLELLFRYGMNRPWMEEARRKGGEDPEY"
     gene            24907..25425
                     /locus_tag="PLG14_00565"
     CDS             24907..25425
                     /locus_tag="PLG14_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOX47647.1"
                     /translation="MPSIAKANREVPLLEDLIETYKGKPGGLLAILEAAQEAERLRYL
                     PEEALRRVAEGTGTPLAQVYSVATFYSFFNLKPQGLHSVVVCRGTACHTRGSLELLEA
                     ALERLGIQGFREDEENSATTADGFASVRTVACFGQCALAPVVMVDGAIKSRMTVGKLA
                     ALLDRLRKGGEA"
     gene            25422..28664
                     /locus_tag="PLG14_00570"
     CDS             25422..28664
                     /locus_tag="PLG14_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47648.1"
                     /translation="MSADKKKTAEGPTDVGALRLLGLERIRPARPFLSVGMGTCGLGN
                     GAAEVFAALEAAVAERGLDAAVRRVGCFGFCAAEPLVMAYRPGRPLLLFTEAKASRAA
                     KLAAGVADDEAFDRLAKLAAAKIESWDFRTSALEFGAAYPFLPRWDELPFFKGQEKIV
                     LRDAGLIDPESIEEYLAAGGYSGLVKAITTMRPETVIEEVRKSKLRGRGGAGFPTHRK
                     WELARAAPGDLKYVVCNADEGDPGAYMNRNEIESDPHALVEGMAIGAYAMGAAEGLVY
                     VRAEYPLAVERLELAIKAARRAGLLGRFILGTKFSFDIEVATGAGAFVCGEETALIAS
                     AEGKAGRPSPRPPFPAQKGLYGRPTNINNVETWCNVPVIVARGGDWFAGFGTEASAGT
                     KVFSLVGKVRNSGLVELPLGTPLEAMVYGMGGGAGPRKRVKAVQSGGPSGGCVPAWLF
                     KTPIDYEELAKIGAIMGSGGMVVMDQDNCMVDVARYFVGFTAGESCGKCAPCREGLSQ
                     MRRILESVARGEAAEEDLATLEELALVVRDSALCGLGQTAANPVLTTLSHFRGEYLRH
                     IREKRCEAGVCESLYLALCENSCPLHMNIPGYLQLLKENRIEEAFELTLRENPLPGTI
                     GRICHFHCRMRCRREQLDEPVSQGEIHRYLADTMYKMGRERAVYNRLVKEKLPPTGKT
                     VAVVGAGPAGLAAAFWLARLGHEVTVFDAASEAGGIPRWGIPAYRLPKEVLRKEVAFI
                     RRLGVRFVFNTRIGPDLSWERLAAMSDAVLVAAGATEDISLGVPGEGLEGAVPGYRFL
                     EEINSGRRKSVAAELVVVGGGNSAVDAARSALRMGSRVTLAYRRSRLDMPANAEELEG
                     ALEEGVELLCMAQPLEILGDAEGKVRAVKVARMKAGPVDSTGRPSPVPTGESCELPCG
                     AVVVAAGERVGVPGLEVLGVERERDGRIKADPITFRTANPKVWAAGDAVTGPATAAEA
                     MGQAKAASVAIDEALAGAGRFERLFRRFDYSMAAPKEPSSAKGTKTRKLPVADRKGSF
                     VEISLGYTGEEARIEAERCLRCDVREGARTPLGFAGPACAPKDGAGRLRR"
     gene            28661..30472
                     /locus_tag="PLG14_00575"
     CDS             28661..30472
                     /locus_tag="PLG14_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOX47649.1"
                     /translation="MSGETVSLFVDGRKTVVPAGTGLVEACAAAGARVPTLCYLEGVS
                     ANASCGVCVVEVEGAKSLVRACTAKAAEGMKVRTASERVLRARRTVVELLLANHPADC
                     LVCSRNGACELQALAEDLGPRKLRFQATKRAHAPDLSSAGLARDDSKCILCGRCVAVC
                     QEVQTVSAIQFAGRGIRTRVSAFMDRGLAGSPCVQCGQCSVVCPTGAIAERDETEAVE
                     RAVAKEGLVVAVQTAPAIRASLGEALGMEPGSLVTGKMVAALRRLGFDKVFDTQFTAD
                     LTIMEEGSELIERLTKGGVLPMITSCSPGWINFIEAFFPALLPHLSSCKSPQQMFGAV
                     AKTFFAEKAGIAPESLRVASIMPCTAKKGETRRPGMDAAWRWWRGKGRELSAFPDVDW
                     ALTTRELARMIRRAGIDMASLPEEDFDDPLGRSTGAATIFGTTGGVMEAALRTVHELV
                     NGAPLPGLAFAPARGLEGIKEASLVLGGKELKVAVAHGLKNARALLEGIAAGTSPYAF
                     IEVMTCPGGCVGGGGQPHAFAASRGATARGPVAQGVAAKGLSARNLAMYREDERLGLR
                     KSHENPAVAALYADFLGKPLGHLSHELLHTEYARREF"
     gene            complement(30563..>31765)
                     /locus_tag="PLG14_00580"
     CDS             complement(30563..>31765)
                     /locus_tag="PLG14_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX47650.1"
                     /translation="FNKSHAAAYSVVAYRTAYLKANFPAEFMAANLTNEIANTDKLTE
                     YIGEARSMGLELLPPDVNRSDAYFGVSEGKIVYGFLGIKGVGDGLARAIVEERAKGGP
                     FCGFVDFVERMGSGGMNKKTLECLALSGCFDSFGRPRQELVLNVERAADYAAKKEEAG
                     RYGQVSLFESCAEEEFPPFVLEPAEEYPRSELLRLEKELLGFYFSGHPMDEYRKTWER
                     CSEADLSRPERSSPERLYTIVAMLKEFREILTKNGKKMAFGAVEDYRGSIEIVVFSDV
                     LERFRERFVVDKVLCLKGKIDLTRSAPSFKVEEFADPADLKDKSWREVHVRLAPGIAS
                     EDELYDLRDALFEAPGACAVFFHVPAPDGDSKEVLVRANPQISCSAGEEVLERLKEVR
                     GVVEAWRD"
BASE COUNT         4343 a        11275 c        11748 g         4199 t
ORIGIN      
        1 gccgcggccg ccgcctacga cgccctcgcc gaggagggca agcggctttc cgccgccgcc
       61 gagccccaag ccgccagcgg tgccgcgggc ggctccgcga tggccgcgcg gggcggcggc
      121 tcttcatcca cgccccccgc cgggcccgaa gcaggaaccg ccgcggcgaa gcggcgactg
      181 ccggtctcct accggccgcc cctcgaaccg cccaagggcg gcgcgaaggg caggctcgtc
      241 ctcgtgatcg acgacgtcgg ctacagcctc tccgagctcg agcccttcct cgccctcccc
      301 tttcccttga gcctcgccgt cctgccgggg cttccccatt cggaagaggc cgcgaggcgg
      361 atcagggccg ccggcaagga gctcctcctc caccagccca tggaggcctc ggggggccag
      421 gatcccggcc cgcgggcaat ctacctctcg accccgcccg aggaggccgc ccgcctcctg
      481 gaggagaacc tggactccct gcccggggcg aagggcgtca acaaccacat gggctcggcc
      541 gtgacccggg aggagaggct catggagccg gtcctggagg ttgtcaaaag gcggggaatc
      601 tactatcttg actccttgac cgcatccggc accgcgactt ccggcgtcgc atctcgggag
      661 cgaatccgat actgggagcg cgacgtgttc ctcgacaaca gccccgaccg agtctcgatc
      721 gtccgctata tcgaagaagg caagaagaag gccgagaagg gcgggcccgc cgtcatgatc
      781 ggccacgtct ggtcggccga cctggcgcag accctcgccg acctctatcc cgagctcgtc
      841 gccgagggct tctccttctc caccatatcc aagatcatga tcgaagaggc cgatgagaat
      901 actggggatt gagtcctcct gcgacgagtg cgcggccgcc gtcgtcgagg acgggcgccg
      961 cgtcctctcc gacgtcgtcg ccacgcagat acccttccat gcccgcttcc agggcgtcgt
     1021 gcccgagata gcgagccgca agcacaccga gtgggtctac ggcgtggtcg agaaggccct
     1081 gtccgacgcc ggcaccgccc ccgcggagct cgacgggatc gccgtcaccg cccagccggg
     1141 cctcgtgggc tcccttctcg tcggcatcca gttcgccaag ggcatggcct actccctggg
     1201 gaagcccctc gtgggcgtca accacatcct ggcccatctc tacgcccccc agctcgagtc
     1261 ccccgtcgag tatcccttcc tcggcctcct ggtctcgggc ggccacacga tcatctgcaa
     1321 ggcggaggac ttcgaccgga tcgaggtcct gggcacgacg atcgacgacg ccgccggcga
     1381 ggccttcgac aaggtctcca agtactacgg cttcggctat ccgggcggcg ccgtcatcga
     1441 cgccctggcg gagaagggcg acgacacggc cttcgccttt cccaagccct ccctccacaa
     1501 gggggagcac cgctacgacg tctcctactc gggcctcaag accgctgtca tccaccagct
     1561 ggagcagtac cgccgcgaag gcgcgccgtc caacgccgag aacatcgccg cctccttccg
     1621 caaggccgcc gtggacatcc tcctctcgcg gctcttccgc gcggtggagg acacgggcct
     1681 caagaccgtc gtcgccggcg gcggagtggc cgcgaacgcc tatctccgcc gcgccctcgc
     1741 ggcgaggagc gacctggacg cgcgcttccc tcccctcaag ctctgcggcg acaacggcgc
     1801 gatgatcgcg ggactgggct gccgcctcct cgcgcgcggc gaccgctcgg gcttcgacct
     1861 caacgcggcc gcccgcgtgc cccttttccg caagccccag cgctgagttc ggcggctcgc
     1921 ccggcggcca gtcccgtacc ggcctccctc aagcgggccc ggcctttccg cgcgtagccc
     1981 ccttagggag gccgccatgg gaaagacccc cgtcatcgcc gccgttctcg ccctcgccgc
     2041 gatcctgaag gccgccgcct cgccggcgcc cgtccaagcc ccgccctact acctggagtt
     2101 cctcgccggc ccgacgctcg gcctggaccg cgtcctccgc ggcggctcgg cagccctggg
     2161 cgcgggcttc gcgccgccct gcctgggcgg cctcggcctg ggcgcgaggg ctgaggcggc
     2221 ctacgacgcc ggccttcggg ccttcatcgg cagggctgaa ctcctcctcg ccctgggccc
     2281 gggcctggcg ctgggcctgg gcgcggagct ccccctcggc gagctctccc tgcgcgagcc
     2341 gaggagcggc tgcggcatcg gcctcgagcc cgcggccctg ccctcccgct tctccctcga
     2401 ggccctcgtc gcggagggca aggcgagggg cgggggcaag ccgcgggcgg gcctctccgc
     2461 agagctcgcc tggtcggcct accgggccct cggcgtcgag cccgagggcg agggcgccct
     2521 gccctccctc gccgaggcgc tctccgccga gtcgggcttc gccgcgggac tgtcctgcgc
     2581 cctccgcctg cgcctggcct ggggcccggc cttctaggcg cgcacaatgg agtccaggcg
     2641 gctgccaagg cggcttcggg cccgcagcct ggcgccgcct cgccaccgct accgccacgc
     2701 tgcctagccg ccgcctcgcc accgctaccg ctaccgccac gccgccttgc cgccgcctcg
     2761 ccgcctggtc gcctcgccgc ttcggcgaac gcccgccttg acaaggcccc gtccgaaggc
     2821 gtatcccggt cggatgaaga gccccatgag cgattccttc tcctcctccc tcgagctcgc
     2881 gcccgccctc cgttccgcgg cggccgtcgc ctgccgcgac tcgctcaagc tcaggccggg
     2941 agagaaggtc ctcatcgtca cgaatccctt gcgggacgtg ctcgagatct ccgcggccct
     3001 cttcgacgcc gcccgcgagg cgggcgcctc gccgagcctc ctcgtccagc ccgtgaaggc
     3061 ccagatggac tacgcggagg agtccgtcat cgcggccttc gaggcccagc ccgacgtcgt
     3121 gatgtcgatc tccgcggaga agctcgggaa ggaccgcgag ggcatacgga cgccctacga
     3181 atgggacggc acgagctatg accacatctt ccactaccag ctccgcggcg cgaagaccat
     3241 ccgcgccttc tggtcgccga acgtcacccg ggagatgttc gcccggaccg tccccctcga
     3301 ctacgccgag ctcggggagc gctgccgccg cgtcgcggcc gtcctcgcgg gcgccgagga
     3361 ggtccgcgtg acgaacccga acggcacgga cctgcggctc ggcctccggg gcaggaagcc
     3421 gcttcgcgac gacggcgact actcccgccc gggctcgggc ggcaacctcc ccgcgggcga
     3481 ggtcttcgtc tcgccggagc tgggcacggc gaggggcctc gtcgtctacg acggctcgat
     3541 agccgaccag gcaggggaca tcctcatccg cgagcccatc cgctgctcgg tcgaaggcgg
     3601 cttcgtcgtc gacatcgcgg gagggtccga ggcggcggcc ctgcgccggg ccgtggacac
     3661 ggccgcggcc aacgcccggc gcatggcggc cgacggcctc ctttcccccg ccaaggccga
     3721 ggcctacgcg cggaacgccc ggagcgtcgg cgagctcggg atcggcctca accccctcgc
     3781 ctccgtcgcc ggcaacatgc tggaggacga gaaggccttc aggacctgcc atttcgccat
     3841 aggctcgaac tacgacgagg acgcccccgc cctcatccac ctggacggcc tcgtctcccg
     3901 gccgaccatc gtcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggat
     4021 tcgctagagt actaggcgaa gaaggccatg ggggccgtct tcagcaggcc gtcctggcct
     4081 gccggacccg ccggtccgaa tctcgatcct ccaggaggac gccatgtatc agctagcagc
     4141 cgttgccgcc atcggactcc gggacgatcg ggatagatag ggtcctctct cttcgccttc
     4201 cctacaatcc agacaactcc cgccagtccg aaaggcaatg acgttcggcg cgccgccctg
     4261 cggtgccccg cccgcggttt tcccgcgtcc gggcacgaag ggcagcgggc tcgaggcgcc
     4321 acggcttcgt agccttccag cccatgggcg aaggccgcgg agccgccgtc gggcccgaag
     4381 cgaggccgaa tcccagggtt cccggccagg agcgcggcga cgcgccctca aggccggacg
     4441 ccctcgcggc gcggaagccc gccgtccgtc ggacggcggg gccgcggccc gcggggcgcc
     4501 ttggacccgc gaagtaaaca acggtacggt atcatgttcc atatccatgt agaattcgac
     4561 gacaacgagg gcgctcccag gcgcgacgcc ctgcgcgact tcggcttcga cgagagcttc
     4621 ctccgcgact acctcgcgga ggccgagctc ggcgacgcgc cgggccgcgt ggtcgaggtc
     4681 cagaggggcc gctgcaccgc ggtgacaagg ggccgggacg gctccctttc cgaggtcgag
     4741 gccgtcgtct ccggccgctt tctccacgag gccgagtcgg ccgccgagct ccccgcggtg
     4801 ggcgactggg tggtccttcg cccttccgag ggcggggccg cccagctccg ctccgtcctc
     4861 ccccgcagga gcgccttcgc gcgccgcgcg gccggcgtag cggaggacag ggccgaggcc
     4921 caggtcctcg cggccaacgt cgacacggcc ttcatcgtcg cggcggcggg cagggactgg
     4981 aatccccgcc gcgtggagcg ctacgtcacc ctcgcctacg aggcgggggt tgagcccgtc
     5041 ctcgtcgtga ccaaggccga cctggccgag gacgccgaag gcctcgccgc ggaggcggcc
     5101 gcactgagcc ccggcctcgc ctcggccctc gtctgcgccc ccgaaggaag gggcctcgaa
     5161 tgcctcaagg cctggctccg gccggggaag accgtcgtcc tcctcggctc ctcgggcgcg
     5221 ggcaagtcca ccctcctcaa cgccctggcc gggcgcgagc tcgccgacac gggctccgtc
     5281 cgcgcggacg acgagcgggg caggcacacg accacgcacc gggagctgta caggctcgac
     5341 tcgggcgccc tcgtcatcga caatcccggc ctccgcgagg tcgggatcta cgcgggcgag
     5401 gagtccctcc aggcggcctt cgccgacatc gaggccctgg ccgcggaatg ccgcttccgc
     5461 gactgcgccc acggctcgga gcccggctgc gcggtgcgcg ccgccctgga gtccggggag
     5521 ctcgacaagg ggcgctactc aggctggctc aagctcggcc gcgagatcgc ccacatgaac
     5581 gcccagccgg ggagcgccga gcagcgcgcc cgcgacgccc gctggaaggc catcaacaag
     5641 tcgatgcgcg gctacacgaa ggagcggcgc tccatccagg gccgcgcgcg ctgaccggtc
     5701 gggcggccgc ccgaccggct tggcgcccgc cgccgggaac gcggcgcggc ccgaggctgg
     5761 ccgcccggcc aggcggcgcc gacggcgagg acgatcgtcg cggctgagcg ggcggcccga
     5821 ggcggcgcgt gcgctccgcg gcggagcgga cggctagagc ggcgcgccga gggagcccag
     5881 gtaggactcg aggaactcga gggccgcgag ctcgcggttg aggatgcggt agaccccgtc
     5941 catgatggga aggtcgagcc gcttctcctc cgcgagggcc ctgacctggc cggccgcgac
     6001 cgcgccctcg ggcaggtagc cgacctcgcc tatactcgcg accaggtcct ctatggagga
     6061 gaagcgcgag aggatcccct tctccacgat gtcgcggccg aagcggcggt tcctgccgtg
     6121 caccgagcgg caggtgacgt ccaggtcgcc gacgcccgcc agggaggtga aggtctcggg
     6181 atggctcgcg cccagggcgc gcccgaggag ctgcatctcg ttgaggccgg cggcgaggag
     6241 gagggactcc gtgttgtcgc cgaagatgcc cgccgccgcc cccgcggccg cgccggaggc
     6301 ggcgcccgag gcgaaggcgt ccagcatgcc gaaggctatc gccacgacgt tcttggtcgc
     6361 ggcgcaggcc tggacgccca cggcgtccag ggaggggtag acgaggaccc ggctcccgcg
     6421 gaagagctcg cgggtcctga tcgcgttgcg gccgctcgcg ctggccgcgg ccaggcccgt
     6481 gacgcggccc cgcgagacct cctccgcgtg gctcggcccg gagaggtaga cgagctggtc
     6541 gcggtagcgg ccgggcagcc gctcctccag cgcgtcgagg atgagcctgg gcccccgggg
     6601 cccggggatg aagcccttcg tgacgacgac gacgaggctc tccccttcga tcaggctcgg
     6661 cgcggggagg agcttccgga cgacgcccat gaggtagggc gagggcaccg cgaggacgat
     6721 gacgtcccgc ccctcggcgg cggcgaggag gtccgtctcc gcgcccaggc cctcggggag
     6781 gaggacgccc gggaggtagg ccgcgttctc gcgcctcgtc cggaccgcct ccgccagggc
     6841 gggctcacgg caccagaggc agacctcgtg gccgagctcc gcgaggacct tcgcgatggc
     6901 cgtgccccag gctccggccc cgactaccgc gaccttctcc ggcatggcgc ctacctcccc
     6961 gcttccgcga cgagccgctc gtactcctcc gcctcggcga agaagacgga gaggccgctc
     7021 cgccagaagc cgagggactc gatgtcgaag cccgcggacc tcgtcacctc cacggccgtc
     7081 gcggagcccg tcccggccag gagggcgcga taggcggaag cgaaggccgg cccctccttc
     7141 gcgcctcgcg cgtagagccc gaggccgaag aggaggccga aggcgtaggg gaagttgtag
     7201 aaggagaggt cgggccggta gtagtggccc ttggcggccc acatgtaggg atggaggcgc
     7261 tccccgtcca ggccctcgcc gtaggtgcgg cgctgcgcct cgagcatgag ggagcagagc
     7321 tcctccggcg gcagctcggc cttcccgcgg cgctcgaaga cggcgctctc gaaatagtag
     7381 cgcgagagga tgtcgcagat cacctggcag ccgtcctgga ggtgcgtctc caggaggccc
     7441 aggcgctcgg aggccggggc ggccctggcg gcctcgttcc agacgacggt ctccgcgaag
     7501 atggaggccg tctccgccag ggtcatgggg tactgcgtga gggcgtaggg cttcgacttg
     7561 acgcactcgt ggtgccaggc gtggccgagc tcgtgcgcca ccgtcgtgag ggaggagaag
     7621 gtgccgtcga agttgcacag gaccctcgcc gccttggcgt cggggaagtc gatgcagtag
     7681 gccccgccga ccttgccctc gcggggctcg gcgtcgatcc agcgctcggc gcgggccttc
     7741 ctcgcgaaat cgcccatcgc gggatcgaag gcggagaact tctcggcgat gtagtccagg
     7801 gcggcgtcga aggtgtaggc gggctccccc gagggcttcg ccgcgccgtc cttcgccgga
     7861 tcgtccttcg ccgccggggc gacgggggcg aagaggtcgt ggaaggccag ggagccgagg
     7921 cccagggccc gggccttcgc cttgaggtag cggcgccagg tcgggaggga ctcctccatc
     7981 gcgccgatga gggcgtcgag ggtcgccctc gtcatgcgcg actgctcgat ggacttgtcg
     8041 acggcgcctt cccagcccct gcgccggttc agggtgaggg tctcgccctt cacgccgttg
     8101 agcgcggcgg ccatcgggat ctcgacggcc ttcagggcct cgagctccag gcggtaggcc
     8161 ttctcgcgca cggcgcggtc cgcgtcgtag gccaggttgc ggagctccac gaccgtcttg
     8221 cgctcgcccg tcttcgcgtc ccagaggacg gaggcgttgg agaagagggt ctcctggagc
     8281 cggctccagg cctcgccgcc cgaccgggag aggtcctccg ccaggtcctc gagctcgggg
     8341 gacatctgct tcgactgcca gaagagctcc tcgcgcaggt ggaaggcgag gggcgcgatc
     8401 cgggggtcgg cgccgggccc ggcggccagg gactcgacct cggaccgacg ggcggccagg
     8461 gcgttcctga ggaggacgga ggcgcgcttc aggggaaggc cgagctcctc ggaggcgttg
     8521 agctcggcca tggccttcgg gtcgcgtgtc gaggtcgagt agctcgcgta ggcgtaggag
     8581 gagagggtct cgtagaggga gcccgcctcg ccctcgagct cgagggcctt ggcgagccag
     8641 tcggcgaagg ccgcgcgctc cgcggggccg gaagccgcgc cgcccgaggg ggccgcgggg
     8701 gccgcggggg ccgcgcccag gtgggcaagg aggccggcgg agaggcgcgt taggcgctcc
     8761 ttggcggcgg cgtgctcggg ggagccgaag ccggggtaga ccggcgccag gtcccagcgg
     8821 ggaagcttct cagatccagc catcgttgcc gtccttccag tcgatgatct cctcgggcct
     8881 gaagaagagc ccgatctcgc gcccggcgct ggcggccgag tccgaggcgt ggatgatgtt
     8941 gagtccggtg tgcatggcgt agtcgccgcg gatcgtgccg ggcgcggcct cctccgcctt
     9001 cgtcgagccg cagagcttcc gcagcatgga gacggccccg tcgccctcca gcaccatcgc
     9061 gaccacgggc ccgctcgtga tgtagtccac gagctcgccg aagaagggct tgtccctgtg
     9121 ctcggcgtag tgggcctcgg cgagggggcg cggcaggcgg acgagcttgg ccgcgacgat
     9181 gtcgaagccc ttgcgctcga agcgcgagat gatctcgccc gcgatcctcc gggaaagaac
     9241 gccgggcttc agcatcgtga aggtccgctc gagcgccatg gccgcctcct cttcgtcgtc
     9301 cgtcctcggc gccaatccta gcacgggagg ggcggacgcg ccagcctcgc ggcccggccg
     9361 cggctctccc cgagccccaa gcctcgagtc ccgggcccag cgcggcggcc cgggcaaggc
     9421 cgcgccggtc cttgcggcgc gccgcgcgcg gagccatact tccccgcgag atgaactcgg
     9481 aattccatta ctacgccgtc catttcctct gcctccgctc gggcttcgcg cccgaggagg
     9541 cgaggaggat cgcgcgctcc tcccagtacg tcgacgacgc ctcggtcgcc tacgtcgtcg
     9601 aggacggccg ggcgccctac cgctgcctcg tgacccagaa ctacgtcttc tgggacgagg
     9661 cgaccctgcg ggaggtctac ctgcccttcc acttcctgcc cggcgatccc gagcgcgcga
     9721 ggcgggagag gcgcgacggc cgtgcccacg agctcgcggc gagccccgac tcgcccttct
     9781 ccaaggagct cctcgtcgag gccctgagga gcggcgaccc ctatcgcctg gggatagccc
     9841 tccacgccta cgccgacggc tgggcccacc agcacttctc gggcaggatc gaggaggcga
     9901 acgccctcga cccctcctcg cccctccctc ccgcgggcca cctccaggcc ctgcgctccc
     9961 ccgacgaagc gctcggccgc tggcacgacc cccgcctctt tcccgagttc gccgaggtga
    10021 acaaccgccg gcgcttcctc gaggcggcga agaagatcta ccgctacctc cgcgtctcgc
    10081 ggcgcctccc cttcgacgac gaggagctgg tcctgggcga gctcgagggg atctgggccc
    10141 gcaaggccgg agaccccggc caggcggcgg gcgcggcgcc gagcgccgcc caggccagga
    10201 cggcggccag ggcgaggctc gacgagttcg tcatccagtt cgacatggag ccctacgaca
    10261 agcgggaatg gctgcgcgag gcggggatag ccgttcccgg cggcgacgag gagcccttcg
    10321 tcggctacga caagctgcgc tggctcggct cgcgcctcaa gtcgggcggc gccgcgcccg
    10381 agctccgcgt aggttcgggc ggccgcttcg aggactcgga gctcaggcgc tgggacgagg
    10441 ccgcccgggc ccacctccgc gcggccaaga gtctcatgaa ggcggctgga atcgcggagg
    10501 cccaaggatg aggccgctga aactcgcccg cgccgctccc gcttccgtcg cctccgccgc
    10561 gccagccgcc ctcgcggcgg cccgcgcggc cgcgcgcgcg tcggccgctg ccctcgcggc
    10621 cgtcatcctc ctcgggggct gcgcggcgcg gatccgctac gagacgcccg tcgatcccct
    10681 ggccgtcctc gccggggacg ccttcctcta cgcgaggatc cgcgggccct cgctcgccct
    10741 cctcgctccc gccctcctcc cggaggagga ggcaagggcc atcgcccccc tcattgcgcg
    10801 aagccgcgag ctcggcctcg cgctcacgcc gcccggcgag cccggggaaa ggccgggcct
    10861 cgagggcctc ctggtcggcg actacccctt ccgctcggcc tctttcgccc tctcgggcga
    10921 cggagcctgg aagcgcgagg gcccgggcta cgccaacgcg gcggccggcg tcagggtcgc
    10981 ccttccgggc ccgaccgtcg tcctcgccgc ctcgggcccc atcgagcccc tcgtggctcg
    11041 gctcaaggcg ccgggccccg agcccatccc cgcgcgcctt gcccccctcg caggacgaga
    11101 ggtcgtcctc tacgcgcccc tccccttcga tcgcctcgcg gagagcctcc tgggcgagcg
    11161 cctcgacatc cccgcgcgcg gcctcatcct cgccgccgag cgggcgggcg cggtcccggg
    11221 cgcgcccgaa ggcgcgggca gcgaggcggc ctacgacctg gccctggcct tcctcatgga
    11281 ggacgaggac tcggcgcgga tctacaaggg agccgcccgg ctggcctggt acgccctcgt
    11341 gcgctcgctc ttccccgagg aggcggaggc catcctctcc cggcgcatcg aggccgaggg
    11401 cgacctcgtg gctgcggcgg gcatcagggt cagggcctcg agcctggccg gcgccctctc
    11461 gaggctcggc ggccgctgac aacggcgggg ccgccgagga ggtcgcccct cgcgccggcc
    11521 gcgccgcggc tggttggctc cccgaggccc gcggcgcgct cctgagaggt ccggcgcggc
    11581 cgccgctgac cgcggccttg cggagctact cgccgccctc ttccccggcg ccctgcccct
    11641 cggccccggc ctccccggcg ccgggaaggg acggaaggac gccgccgtcg acgcggagga
    11701 ggccttcgtc gggcgcctcg ccgcgcgcga aggccgcgag gagggcgccg aggccctccg
    11761 cgaggggaaa gcccgggtcg ccgaaggaac ggtagttctc ctccaggtcg ggcccgacct
    11821 ccttgaggac gtggttcatc cccggaagga cgacggcggc cgcgtcgggc tcaagcgcgg
    11881 cgaggacgga gaactccgcg aggctcacct ggaagtcgcg gttcccctgc acgaggagaa
    11941 gttgtccggc atagatcgag agctccttcg gaaggtcata gcggaaccag gaggccaggt
    12001 agggctggaa actcggcctg aagaagtccg cgaagtaggg cgagggctcg ggataggtct
    12061 cgccgcggcg cagggccgcc atgatcgcct cggcctcggg cctgagctcc tcgggcgcct
    12121 cggccagggc ctcctcgacg ctctccgccg cgctcttccc cgagggacag aggagggcga
    12181 ggccggggac ggacttgccc aggcgggcgg cggccgccgc ggcgacgagg ccgccctcgg
    12241 tgtggccggc gaggacgagg cgggagaagc gcccgtccgc tcccagggcc gccgcgacgg
    12301 cggcgcagtc ggccacgtag tcgtcgaagg aaagctcggc ctcgctcgcg acgagggcgt
    12361 agctctctcc cgcgccccgc ttgtcgtagc ggtagctcgc gacgccctct ccgcgcaggg
    12421 cgagggcgag ggccctgagc ccgtcgttgc ggccgggcac gttgtagttg ttgccgtcgc
    12481 ggtcggtctc gccggccccg gccacgagga cgacgagggg gaaggggccc tctccctccg
    12541 ggaggaggag gctgccgcgg aggacgcccc cgcgcacggc gacctcgagc gccgcgccgt
    12601 aggccgcctc cggcggcgag agggcgagct cgaagaggcc ttcggcgccc tgtcccgaaa
    12661 ggcgccccga gatcctgggc cgcccgtcgg gcgcggcctc ggcgacggca tctcccgcgg
    12721 cgcgcgtccc ctcggagccc gccggcgccg ctgcggcggc tccgtacgcc ggggccgcgg
    12781 cggcttccgg ggccggcgct gtcgaccggg ccggcgcggc ggcctgagcc gcggcggcga
    12841 ggctccccgt gaagaggaga gcgccctcgc ccccgccgac gaggaaggcg acggaggccc
    12901 cctcgacgcg gatcgaggcc aggggatagc cgtagaggcg ctggccgggc aggtcgagga
    12961 ggccgccgcc gccgggaagc agccgcaagg agaagggtgc cttgccctga ccgaaatcga
    13021 gccagccgtc ccagcggccg gaagggacgg cggacgccgc ggccggtgct gtcgaggctg
    13081 ccggggcgtc gacggcgccc gcgggctcga tccgcgcggc cggtgtcgcg gtcggcgcgg
    13141 gcgcggcctg cgccgatggc gcggcggatg aggaggcggc ggcctgcgcc tccccgccgg
    13201 ccggaagggc ggcacagagg gccaggaagg agaggaggat gaaaggcgcg atgcggcggc
    13261 tctgcttcat gcgcgcattc tagcgcgggg cggaggcccg cgcctacgag gcccgcgccc
    13321 ccggactccg ttccggcgga gaccacgtcg cgggggccga gacgcgcgcc ggatacgtcc
    13381 gcgccgcgga ggcccgagcc caaggtcgcg cgcgggcgag gctagcgttc gttcaggacc
    13441 tcgaagcggc gctcggcctc gcggccctcc tcgtcgacga gggagaggag atgccagccg
    13501 gggcccggcc gcgcctccat gcggtgggtc ccgcgcgtcg agcccaggta ctccccgtcc
    13561 aggtgccaga agacgagggc gcgggagtcg cggtgcaccg cggagaagac ggcggctccg
    13621 ggccggcccg agagctctat cggcacgtag agcgaggcgc cctccccggg ctcgaggagg
    13681 gcgaggccgc cgccgccccc agcgggggcc ggcgtccccg gcttccaggg cgggaggggg
    13741 cggtagtcca ggtgggcgcg gcggtagtac cactccatcg cgggcggaag gacgaagcgg
    13801 cgctcggtcc ggatgaggtc gagccgctcg ccctcggcgg tgacgcgata ggcgccgtcg
    13861 gcggagagct ggacgaggcg gcagtagggg caggccgagt cggccttcgc ggcggcgccc
    13921 gcggggagga ggaccctctc ggtccgcgcg cagtcggggc cggcggggag ccccgagtcg
    13981 gcgcagaggg tcgcgtactc gagggcggag gagggcctcg ggatccaggg gctcctcggg
    14041 agggcggcga cgaggtcgaa gaggagggga gccgcggccg tggagccccg gagctcgggc
    14101 ctgccctccc cgctcgcgtt gcccgcccag accgcgacga cgtactccga ggtgaggccg
    14161 acggcccagg cgtcgcggaa gccgaagctc gtgcccgtct tccaggccac cttccgggcg
    14221 gaggcgaact ccctccagga ggcctcgtcc tcgggacgcc ccacctcgag gagggcctcc
    14281 agggtgagcc aggccgcgcc cggggagaag ggatcgggcc tcgcggcgcg gcgcgaggcc
    14341 gcctcccgcg tgtaggcgag gtcgaagtac tgccccccgc cgggcgcctc gaggccgcgc
    14401 gcggtccgcg ccagggcggc gtagcggccc gcggcttccc agagggtgat ctcagccccg
    14461 cccaggatca gcgtgaggcc gtagtcctcc gcgggccgcg tcagggtcgt catgcccgtg
    14521 cccaggagga gtcgccggaa gcgctcgacg ccgaaggagc ggaggaggcg gacgaagggc
    14581 acgttgagcg acctggccag ggcctcctcc gcggggacgg cgccggagta gctccccgtg
    14641 ttgttctccg ggccgtagga cccgacccgg gtcggtatgt ccgcgaggag ggccttgggc
    14701 gtgatctccc cggagtcgag ggccgccgcg tagaggaagg gcttgaggag gcttccggag
    14761 ctgcgcggcg agcggaccac gtcgacgcgc tggccccggt cctcgtccgc gcccaggccc
    14821 gcgttgccga cgtaggcgag gacctctccc gtgtcgaccc gcgccacgac gcaggccgcg
    14881 ccgtggacgc ccgaggccgc gagccgggcg gcgcggcgct ccacgagggc ggcggcccgc
    14941 tcctggacgc cgggatcgat ggtggactcc acgcgggcgc cggccagtcc cgccgccgcc
    15001 gcctcggccc tggccctgtc caggaggtgg ggggcgaggc tcgggatcgc gaagggctcg
    15061 ggcggcaggg gctcggccag ggcgaggccc aggtcctcct cctcgagctc gccccgggcg
    15121 gcgaggagcc tgaggaggcg gttccgcttc tccaggagga ggccgcggtt ctttcccgga
    15181 tgggccgcgg agggcgcgtt cggaaggacg gccagggcgg cggcttccgc ccaggacagg
    15241 ccctcgggcg gccgcccgaa gtagcggaag ctcgccgcct ccaggcccac gacgttgccc
    15301 ccgaagggcg cgcgggcggc gtagagctcc aggatgccgc gcttcccgag gaggagctcg
    15361 tagcgcaggg ccagggcgag ctcggcgagc ttccggccca tcctgcgctc gcccggggga
    15421 agcgttatgc gcgcggcttg catggagagc gtcgagccgc ccgacacgac ccggcccgct
    15481 cgggcgttct gcagggccgc gcggaggatg gacctcgcgt cgaagcccgg gtgggagagg
    15541 aagcgcctgt cctcgtaggc gaggagggcg cggacgaact tgtccgggag gcggacgccc
    15601 ggcgggaact gccagtagcc ctccgccgag accgaggccc cgaggaggcc cccctcgcgg
    15661 tcgaagaggg ccgtggagag ggaaccgggg gcgaggggcc gcggcggccc ccagaggaac
    15721 aaggccgcga gggcgagggc ggcggcggcc ggcggcaggg tcgcgaggag ccgcccgggc
    15781 gagctccgcg cggcccctcc ccggagcgcc gcgacgctca cggccgcatg ttccgttccc
    15841 gccgcttcgc gtctatcccc gagcgtggcg gctcggagag ccagcggccg ggctcgaccg
    15901 cctggtagcg ggcgtcgtac atcgactcga ccgacgagga ggggaggtag aagctcccgt
    15961 cgtaggtctt gttcgcgtag atcctgaagg tcttggactc gcggccctcc agcgtgaagt
    16021 aggtgaggac ccggtcgtcg cggatgtcct ggtactcgta gaggggcggc gcctccttcc
    16081 tccgctcctc ctcctcgccc tcctccctcg gccgcgggag ttcttctccg acgcggtagt
    16141 tggcgatctc ccagcccgcg gggaggactt gcgtgagggc gaggttcctg agcttgaggg
    16201 tggtcctgtt ccgcacgacg gcctcgatga cgaggtcctg gccgaggggc agggcgttcg
    16261 ggtctatcgg gttctcgtcc atgtcgaagt agcccacgga gatggagagg ccgttcgaga
    16321 cggcgctctc ctggccggcg gcggggacgc cccgggcgac gaccttcgcg aatacgggcg
    16381 tcttggagag gttcttcacc gagtaggcgc cggagacggc ctcgcccggc tcgaggtcga
    16441 tgcgggcgga ggcccggtca agcctgacgc gcctctgcgt cccgtcgccc gcggtcacga
    16501 ggagctcggg cgcctcgccc ttggaggcgc gggaggcgta gggaaggccg gccgagaggg
    16561 cggcgccgat ctcctgggtg gagagccagc gccccggctc gttgagctcc agggcgatct
    16621 ggcggtagac cgggaggccg cgctccaggt cgccgagcgc gttgagcgcc tccaggacgg
    16681 cggcgcgctc gcggaagggc gagccgtagg tgtactcgtc caggccctcg tagacctcga
    16741 aggaggccgg cgcggcgcgg aggagggagt cggccgcctc gcgctggccc gagagggcgt
    16801 aggcggcggc gagcctgtag tgggccgcgg cggggaaggg cttgtactcg cggaagcggt
    16861 tcatggaggc gggctcgggc ttccccgcca gggcgaggac atagagcctg taggcctgga
    16921 cagacttcga ccactccgcc cgggagttcc agagcctggc gccctgggcc aggtaggcca
    16981 gggccgggtc caggacgccg tcgcgcacct cgtagccggc ccttcgggcc gcgaccagga
    17041 agtgggcgac gtaggcggag agccagtcct ggtcggcgcc ctcgccgggc cagaaggtga
    17101 agccgcccct cggcgtctgg tagtcgccga gcttcgcgat ggcgagggag acgttgcgcc
    17161 gcacctcctc gagcctctcg ggcgggaggg cgacggactc cggcaggtag agctggggga
    17221 aggccttgga ggtcgtctgc tcggagcagc cgtgggggta ctggatgagc caagccaggc
    17281 gctcgcccag gttgagggtc gggaggcgcg agagctcgag gctcgccttg ttcgtgccgg
    17341 gggcgccggg ataggcgatc tcggcgctcc actccttgcc ggcctcgacg ccggcctcga
    17401 cgacggcggt gacggggatg ccaaggggcc gcacctcgag gtcgatcgcc tgctcggccg
    17461 agaagccctc ggcctcggcc ctgaggacga ggcgggcggc gcccggcttg gcgccggcct
    17521 tcaccctgaa ggggaccgcg aggtcgccgt cggccgcgta gtccaggtac tgctcgcctg
    17581 gaccggccag ggcggcggcg ccggagacgg cgaggctcac gcggacgcgc ttcttcccga
    17641 ggaaggagaa gaccgtcgcg ggcacggccg cctcctcgcc gggggaaagg aggcgggggg
    17701 cggtgagctg ggccatgagc ttggagcgga cgggcacctc tttctcgaag gcgccgtagg
    17761 cggcgccggc ggcgggagcc gcgggcccgg ccttggccgc gccgacggcg cccgaaggag
    17821 agcctatgga gccggagccc gcgaccacca tgaagcggac ggctccgacg tagggcccca
    17881 tgcggaagct cttcctgacg gtctcgcccg ccttcacctc gaagggcggg aagaactgga
    17941 cgacgggcgg gaagcggctc accttgcgct ggccgccgcc caggccctcg tcgccgccgc
    18001 ccaccgcgag gagggtctcg agcttgccgg agaaggcgcc ggccacgtac tggtagaggt
    18061 cgtagctcgc gagggccgag gcctccttct tgtagaactc gtcccagggg ttcggcgccc
    18121 ggaagcgcgt gatgccgagg aggccctcgt cgaccacggc gacggtgcag gtcatgggcc
    18181 tgccggaggc ctcccggacg gagaaggaga cctcggagtt gggctcgatc tcggagggcg
    18241 cgaggacgag gggggagagg cgggtcgcgg gatcctcgac catcacgggc acgacgccgt
    18301 agaggcggat cggcaggtcg ttcgccgtct gcagatgggg ctggaggaag ctcacatgga
    18361 tgtagacgtt gggcgccatg tcgggggtgg cctggaactc gtagaccgtg cggtcgccct
    18421 tggcgcgcac ccactcctgc ctgacgaggc ggccggcccg ctcgatcgcg accagggccc
    18481 ggccttccgc gttcgagggg aaggagacct ggaccttctc gccgacggag tacttctcct
    18541 tgcccgcctc gaggctcagc atggaggcgg cgccgccgcc ctcgccccgg cccttcccgg
    18601 cgtagccggg ccagtcgacg tagaagacct tgccggcgga gtggaggggc ttcgcggacc
    18661 gccccgacct gggctcggag acgtcctcca cgcggatgag gaagcggccc cagttcggat
    18721 aggcgatctt gaagccgaag gaggcccggc cgccctcgac ccgcaccctc tcgcgcttca
    18781 gggggcgctc gtagatggag ttggccgtct ccgccaggct ctcgtcgccc ttctcccacc
    18841 accagcgcca ttcgagctgg tagagggaga cctcgacggt gcccgagggg acgggcttcc
    18901 cgtcgcggtc gaccagggcg agctcgacgc ggtgctcctg gtcggtgagg agcatgccgc
    18961 gggcctggtc gcccttgggg agcttcaggc cggcgtagcg cccgtagggg tggtagtcga
    19021 actgggcggt ctcgctggag aagacgccgg agggctcgaa gacgcgggtg aggagggtgg
    19081 cgcggagccg cccgggcgcg aggccttcgg gcgagaggtc gacggggaag ctcgtcttgc
    19141 cgtccgggcc gagctcgccc tcgaagagga cggcgcgctc gccggagacc tcccgggtcg
    19201 ggtcgtcgaa gtcgtagtcc ttgaaggagg tgaaggaggt gcccgccgcc tggaaggtgg
    19261 ccgacacgtc ggcgcgcagg gccccggcgg gggcgcccgt gagccaggcc gactcgaggc
    19321 gcatcggcgc ggagtccgcg gccaggtagg gagcgcctcc gagctcgagc ttgaccttga
    19381 ggcggttcgg catcacggtc tcgaccctga tggccttcgt gaagctgcgg ccgccgaccc
    19441 tgacgcgggc caggtagggg cccgtgggcg agtcggaatt cgtggaagtc tctatcgagt
    19501 agaagccgtt caccgcgccc gtggaggtcc cggagcgcac gacgcggccg cgcgggtcct
    19561 cgagctcgta caacacgggc aggcccgcgg gcaccgagcc cgagcggtcg tagaggacga
    19621 aggtgaggtg gatggggtcg cccggcctcc agacgccgcg ctcgccgtag atgaagccct
    19681 tcacgccgtc gtcggccttc tcgccgccga cgtcgaagtg gctcacggcc aggcgcgagc
    19741 ccgggtcgag cttcaggtag ctccgctggc tcccgagggc gaccaccgcg aaggagggct
    19801 ccttccccgg gaggaggcgg accatgccgt cggcgccggt cctgccctcc gaaaggaggc
    19861 ggcgctggaa gttgtagagg gagaccgcgg cgccctggac gggggcggcc gtccggaggt
    19921 cgctcgcggc gagggtccag gcgccgtcgg tctcgagccg cgccgtcgcg cccaggtcgg
    19981 agaccaggac gttgcgcttc tgcgtgatgt cgtggtcgta ggcgggaagg tagtaggccg
    20041 ggtggcaggg gtcgttcttg tacttgtaga actggccgta gccgtcgtcg ccccactcct
    20101 ggacgtagtc ccagaggctg tactcgtcgt cgccgcggtc gacgatctcg tcgccgggga
    20161 actcgaggtc cctgaagtcg tgctccgtcg ggcagacgta gcggatgtgg tccttgcgga
    20221 aggttatgcg gatctggaac atgccgtcct tgtgggcggc caggaggggc gagaggtcga
    20281 ggccctggcg gacccagcgg ttcttccagt cctccttcca gcccaggtcc acggtcttcc
    20341 tccagaccac ctcgccgacg cgccgcacgt cgcaggagga gtcgaggtcg ttgacctgga
    20401 ggaactggag catgttgtcg ccctggaccc gcaccgcctc gatgatcagg gcggagaggt
    20461 tcatcgtctc cacgggcagg gtgaggccct ggctcgtcgg gaggatggcg ctcttggaga
    20521 ggaagcggag ctcgggcttc tcccaggcga aggagacctc cgccgcggcg gcgacggcga
    20581 tggtcgcgcc gccctgggcc ctgaggccgg gctcgacctt gatccgcgcg gaggcgggcc
    20641 aggcggtggc gtagagggag aggcggccgc cctcggggtc gaagcgcagg gactcgacgc
    20701 cggaggcgga gacgaggccg cggaagtcct ggcgccggtc caggggctcg ctgaaggaga
    20761 gctcgacgcg gccgccctcg gccgccctcg cccccaggag ctcgaaggag cccgcgggcg
    20821 ggacgcggaa gtcccgggag ccgccggagg gagcgcccag ggccctgccc ttccagctca
    20881 ccttgagctc ggcgggctcg ggcccgcggg ctatgccttt cacggcgaag cggtggaggc
    20941 gggccgagtc gtggctccag gccacctgga ggccgccggg cagggcgggg ccggccgccg
    21001 cgaggacgcg ctcgacctcg gcggcgccgg ccccctcggc gagggagagg ctcccctcca
    21061 ggacgaggct cccgtcggcg ccggcgcgga ggggctcgaa ggcgacctcg aggcgctgct
    21121 cgacggtcct gaagctgaag ctgaagtagt cctcggtccc gcccttggcg gccttggcgc
    21181 ctgttccggc ctcggcgccc gcggcgccga cggccccgga agcgccgccg gccctgccta
    21241 cgaggaggcc gaggtcgaag cgcaccttgt agagccgccc gggctcgagg ggcctgtcgg
    21301 gcttgaaggc gagggtccgc tcgtcctccc agacggccct gcccttgacg gggggctcga
    21361 ggcggaaggg attggccgcg ggcgaggccc cctcgacgcc ggaggcgccc aggaggacga
    21421 cgcggacggg gctctcgcgg gacacgaggc ccgcggtgtg cgcctccacc ctcgcggggt
    21481 cgggcgcctt ggccttgccg aggcctcccg agcaggacgc ggcgaggacg gtgatcgcga
    21541 ggatcgcggc gaaagagcgg aagttgcgca tggagcctcc gtggcgggtt gcagggcttc
    21601 cctgaaccat accgcgcgcg gaggcctcca tcaaggcggg gcgcgccccg ttccgggcgg
    21661 accgtcccgg gtgggcccgc ccgggacggg acaggcgccg cccggacggg accgggcggg
    21721 atcaggcccg gccggaagcc gaacccaggg ccgcgtcgaa gcgcgtgagg acgagcttcg
    21781 gctcgccgac cccgtcgatg gtgaggagct tccccttcgc ctcgtaccag gcgatgaggg
    21841 gggcggtctg cttccggtag ttctccaggc gcgtccggat cgaggcttcc ttgtcgtcgt
    21901 cgcgggtgta gacctcgccg ccgcaggcgt cgcaggcgcc ctccttcttc gggggcatgg
    21961 tcctggtgtt gtagatcttc ccgcaggcgc ggcagacgcg ccgcgtcgag aggcgggcga
    22021 cgacgacggc gtcgtcgacc tcgaagttga cgaccttgtc ggcgggggcg aaggcctcga
    22081 gggcctcggc ctgggggatc gtgcggggga agccgtcgag gatccagccg gccttggcgt
    22141 cgggggccgc gagcctctcc ctgacgaggg cgatcgtcag gtcgtccggg acgagtcctc
    22201 ccgaggcgag gatgcccttc accttgaggc cgagctcggt cccgtccttg accgcggcgc
    22261 ggaagaggtc gcccgtcgag atgtgcggca gcttgcgttc ctggacggcg agatcggcga
    22321 gggtgccctt gcccgctccg ggggggccga ggaaaatcag tttcatggct gtctccttcg
    22381 cacaacatag cctatggcca ccggaaggta aacgggtcgc cgcggcttgg ccgcggtcgc
    22441 gcggcgcccc ttggccgcgg tcgtgcggcg ccccttggcc gcggtcgcgc ggcgcccctt
    22501 ggccgcggcg acgcggcggc gcgtgactgc gcggcagagg ggcgccctag atctcgtact
    22561 cgggaggcgg gccctctgcc cgcctggact ccgcggccgt ccccgcgtcg gccgccgtcc
    22621 cgtccttcgc ggccgggact ccctccatcc agggcctgtt catggcgtag cggaagagga
    22681 gctcgagccg ccccaggacg gcgtcggtcg ggaggacgat ggtgagggcc ccgcgggcgc
    22741 ggaaatcgct ggacgagggc gtggagaggc ggcgctcgtc gacggcgaag cggagcccgc
    22801 gggagcggta ggcctcctcc gcggccgcga ggcccgcggc cccgccgggg acggcctcct
    22861 cgccttcggc gccccggagg atgacgaagg ccccgaggtc gagctcgacg ccggcgcggg
    22921 cggcggcggc gctccaggcc gcgagatcct cgggaaggta gcgcagggcg aggggcaggg
    22981 cgtcggagcc gaggaggtgg gtgagctcga gccgtccggg ccggcagcgc ccgaggaggc
    23041 gctcgacgac gcctatcgtg tcctcgccct cgcccaggtc gaggggaacg acgaagggcg
    23101 agaggatccc cgcgagctgg agcctgagga gctcgtagcg gaagccgtag tcggtgcgcc
    23161 ggggcttgct cgggttctcc ccgccctccg gcgcgacgag gacgatgtcg gagccgcagc
    23221 ggtccgaggc gaggaagcgc agggccgcgg cgagatgggt catctggaag ggatcgaagc
    23281 tcccgatgaa gaccccgacg cgggccaggg gcgagggcgg gcggaaggcc tcgggccgcc
    23341 gcggcatggc caggggcttg aggcgccctt ccgcgacctt gcggtcgatg cggaggaggg
    23401 cctcctcgaa gagctcggcg tagaagcgga actcccggcg ggcgtcgtcg cccagatagg
    23461 gacgggcgtc gaggccggcg aagatcctgc ggaggcggca ggacaccctc cccgcctcgc
    23521 gcgtcgcgaa acggcgccct tcggcctcgc aggcctcgcg tcttccagcc acgcctcaag
    23581 tatagcggaa cctcgatgta cggcaaggcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnagtactcg ggatcctccc cgcccttccg ccgcgcctcc tccatccagg
    23761 gccggttcat gccgtagcgg aagaggagct ccaggcggga gagaagggcc ggcgagggga
    23821 aggctatcgt cagggcgcct cgcgagcgga agtccgtgct ggagggggcg ccgacgcgcg
    23881 aggtgtcagc ggcgaaacgg acgccgagcg cccgggccgc gcgcgcggcc tcctccggcg
    23941 gctcgtcctc accccgcggc acgacgaaga ggccgagctc caggtcgacg cggcagcgtt
    24001 cggcctcgga gcgccaggcg gcgaggtcct cgggcaggag gcggatcgcc gtcggcaggg
    24061 agtccgagcc gaggagatgg gtgagcctga gcgaggcgcc gggatggagg gcgatgaggc
    24121 gccgcacgat cccgatggtg tcggccccct cgcccaggtc cagggggacg acgagggggg
    24181 agaagatccc ctcgagctgg agccgcgcga tctcgtagcg gaagcggtac tcggtcttgc
    24241 ggggcttcaa ggggttggac gccccctccg gcacgacgaa gaccgcgtcc gactcgcagt
    24301 cttcggaggc gaggaagcgc agggccgtcg ccaggtgggt catctggaag gggtcgaagg
    24361 agcccgcgaa gaccgcgacg cgggccgagt agccgggata gcggacgcac tgcggaaagt
    24421 cgcggaggtc gaggggcctg aggcctcccg agaggatctt gcggtcgatg cggcccaagg
    24481 cctccccgat gagcccctcg tagtagcggt actcggccct ctcggcctcg cccaggtagc
    24541 cgaccgcctc caggcccgcg aagacgcgcc tgagcctgcg ctcgatccgg cctgcggctt
    24601 ccgaaacgag gcggcccggg acgggtcctc ccttggagtc cggcttcggg ctctccatgc
    24661 gtttgaagta tagggaatcg cctcgtccag gcaagcggaa ggccgggtga attttatggg
    24721 tatattatta tccatatatt ccaggctttt tctctcccta cctggtcggg tgaaaaaatc
    24781 ttcttttctc gatacttatt tcttgtattt taaaatccat gccttagact ccagggagaa
    24841 acagttcgga gcaccgccgc agtacagctt gtttcgcacc ggttcgctca aaggcgagga
    24901 ggcgtcatgc catcgatcgc gaaggcgaat agggaggtcc ccctgctcga ggacctcatc
    24961 gaaacctaca aaggcaagcc cggaggcctg ctcgccatac tggaggcggc ccaggaggcc
    25021 gagcggctgc gctacctccc ggaggaggcc ctgcggcggg tcgcggaagg caccggcacc
    25081 cctctcgccc aggtctactc cgtcgcgacc ttctactcct tcttcaacct gaagccccag
    25141 ggactccact ccgtcgtggt ctgccgcggc acggcctgcc acacgcgggg ctctctcgag
    25201 ctcctggagg cggccctcga gcgcctgggc atccagggct tccgcgagga cgaggagaac
    25261 tcggcgacga cggccgacgg cttcgcctcc gtgcggaccg tcgcctgctt cggccaatgc
    25321 gccctcgcgc ccgtcgtcat ggtggacggg gccatcaagt ccaggatgac ggtcgggaag
    25381 ctggccgccc tcctggaccg gctcaggaag ggaggcgagg catgagcgcg gacaagaaga
    25441 agacggcgga aggcccgacg gacgtcggcg ccctcaggct cctcggcctg gagaggatcc
    25501 gccccgccag gcccttcctc tccgtgggca tgggcacctg cggcctcggg aacggcgccg
    25561 ccgaggtctt cgccgctctg gaggcggccg tcgccgagcg gggcctcgac gccgcggtcc
    25621 gccgcgtcgg ctgcttcggc ttctgcgccg ccgagcccct cgtcatggcc tacaggcccg
    25681 gccgccccct cctcctcttc acggaggcca aggcctcgcg ggcggccaag ctcgcggcgg
    25741 gcgtcgccga cgacgaggcc ttcgaccgcc tcgcgaagct cgcggcggcg aagatcgagt
    25801 cctgggactt ccggacctcg gccctggaat tcggagccgc ctatcccttc ctcccgcgct
    25861 gggacgagct gcccttcttc aaggggcagg agaagatcgt cctgcgcgac gcgggactca
    25921 tcgacccgga gagcatcgag gagtacctgg ccgccggcgg ctactccggc ctcgtgaagg
    25981 cgatcacgac gatgcgcccc gagacggtca tcgaggaggt caggaagtcg aagctccgcg
    26041 gccgcggcgg cgcgggcttc ccgacgcacc gcaaatggga gctcgcgcgc gcggcgccgg
    26101 gcgacctcaa gtacgtggtc tgcaacgcgg acgagggcga cccgggcgcc tacatgaacc
    26161 gcaacgagat cgagtcggac ccccacgccc tcgtcgaggg catggccatc ggcgcctacg
    26221 cgatgggcgc cgccgagggc ctcgtctacg tgcgcgccga gtaccccctg gccgtcgagc
    26281 gcctggagct cgccatcaag gcggcgaggc gtgccggcct cctcggccgc ttcatcctcg
    26341 gcaccaagtt ctccttcgac atcgaggtgg cgacgggggc cggcgccttc gtctgcgggg
    26401 aggagaccgc cctcatcgcg agcgcggaag gcaaggccgg gcggccctct ccccggccgc
    26461 ccttccccgc gcagaagggc ctctacggcc gccccacgaa catcaacaac gtggagacct
    26521 ggtgcaacgt ccccgtcatc gtcgcgcggg gcggcgactg gttcgcgggc ttcggcacgg
    26581 aggcgagcgc cggcacgaag gtcttctccc tggtcggcaa ggtgcgcaac tccggcctcg
    26641 tcgagctgcc cctcggcacg cccctggagg cgatggtcta cgggatgggc ggcggcgcgg
    26701 gcccgcggaa gcgggtgaag gccgtgcagt cgggaggccc ctcggggggc tgcgtgcccg
    26761 cgtggctctt caagacgccc atcgactacg aggagctcgc gaagataggc gccatcatgg
    26821 gctcgggcgg catggtcgtc atggaccagg acaactgcat ggtcgacgtg gcccgctact
    26881 tcgtcggctt caccgcgggc gagtcctgcg gcaagtgcgc gccctgccgc gagggccttt
    26941 cccagatgcg gcgcatcctc gagtccgtcg cccggggcga ggccgccgag gaggacctcg
    27001 cgaccctgga ggagctggcc ctcgtcgtca gggactcggc cctctgcggc ctggggcaga
    27061 cggcggccaa cccggtgctc acgacgctct cccacttccg cggcgagtac ctgcgccaca
    27121 tcagggagaa gcgctgcgag gccggcgtct gcgagagcct ctacctcgcc ctctgcgaga
    27181 actcctgccc cctccacatg aacatcccgg gctacctcca gctcctcaag gagaaccgga
    27241 tcgaggaggc cttcgagctc accctgcgcg agaacccgct gcccggcacg atcggccgca
    27301 tctgccactt ccactgcagg atgcgctgcc gcagggagca gctggacgag cccgtctccc
    27361 agggcgagat ccaccgctac ctcgcggaca cgatgtacaa gatgggccgc gagcgcgcgg
    27421 tctacaaccg cctcgtcaag gagaagctgc cgcctacggg caagaccgtc gcggtggtcg
    27481 gcgccgggcc cgcgggcctc gcggcggcct tctggctggc ccgcctgggc cacgaggtga
    27541 cggtcttcga cgcggcctcc gaggcgggag gcatcccgcg ctggggcatc cccgcctacc
    27601 gcctcccgaa ggaggtcctg cgcaaggagg tcgccttcat caggcgcctg ggcgtgcgct
    27661 tcgtcttcaa cacccgcatc ggccccgacc tctcctggga gaggctggcc gcgatgagcg
    27721 acgccgtcct cgtcgcggcg ggcgccacgg aggacatctc cctgggcgtg ccgggcgagg
    27781 ggctggaggg cgccgtcccc ggctaccgct tcctggagga gatcaactcc gggaggcgca
    27841 agtccgtcgc ggcggagctc gtcgtcgtgg gcggcggcaa ctcggccgtc gacgcggcca
    27901 ggtcggccct gcgcatgggc tcccgcgtga ccctggccta ccggcgctcg cgcctggaca
    27961 tgcccgcgaa cgcggaggag ctggaaggag cgctggagga gggcgtcgag ctcctctgca
    28021 tggcccagcc cctggagatc ctgggcgacg ccgaggggaa ggtccgcgcg gtcaaggtcg
    28081 cgcggatgaa ggccgggccc gtggactcca caggaaggcc ctcgcccgtg cccacgggcg
    28141 agtcctgcga gctcccctgc ggagccgtcg tcgtcgccgc gggcgagcgg gtcggcgtcc
    28201 ccggcctgga agtcctgggc gtcgagcggg agcgggacgg ccggatcaag gccgacccga
    28261 tcaccttcag gacggccaac cccaaggtct gggcggccgg cgacgcggtg acggggcccg
    28321 ccaccgcggc ggaggcgatg ggccaggcca aggcggcctc cgtcgccatc gacgaggccc
    28381 tggccggagc cgggcgcttc gaacgcctct tcaggaggtt cgactactcg atggccgccc
    28441 ccaaggagcc ctcctccgcc aagggcacga agacgcggaa gctccccgtc gcggacagga
    28501 agggaagctt cgtcgagatc tccctcggct acacggggga ggaggcgagg atcgaggccg
    28561 agcgctgcct gcgctgcgac gtgcgggaag gcgcccggac gccgctcggc ttcgccgggc
    28621 cggcttgcgc gcccaaggac ggcgcgggga ggttgaggcg atgagcggcg agaccgtttc
    28681 cctgttcgtc gacggccgga agaccgtcgt cccggccggc accggcctgg tcgaggcctg
    28741 cgccgcggcc ggcgcccgcg tgccgacact ctgctacctg gaaggcgttt cagccaacgc
    28801 gtcctgcggc gtctgcgtcg tcgaggtcga gggggccaag tccctcgtcc gcgcctgcac
    28861 ggccaaggcc gcggagggca tgaaggtgag gaccgcgagc gagcgcgtcc tgcgcgccag
    28921 gcgcaccgtc gtggagctcc tcctcgcgaa ccatcccgcg gactgcctgg tctgctcccg
    28981 gaacggggcc tgcgagctgc aggccctcgc cgaggacctc gggccccgga agctccgctt
    29041 ccaggccacg aagcgggccc acgcgccgga cctgagctcg gccggcctcg cgcgggacga
    29101 ctccaagtgc atcctctgcg gccgctgcgt cgcggtctgc caggaggtcc agaccgtctc
    29161 cgccatccag ttcgcggggc gcggcatccg cacccgcgtg tcggccttca tggaccgcgg
    29221 cctcgcgggg agcccctgcg tccagtgcgg ccagtgctcc gtcgtctgcc cgacgggcgc
    29281 gatagccgag cgcgacgaga ccgaggccgt ggagagggcc gtcgcgaagg aaggcctggt
    29341 cgtcgccgtc cagaccgcgc ccgcgatccg ggcctccctc ggcgaggccc tgggcatgga
    29401 gcccgggagc ctcgtcacgg gcaagatggt ggccgccctc aggcggctcg gcttcgacaa
    29461 ggtcttcgac acccaattca ccgccgacct caccatcatg gaggagggga gcgagctcat
    29521 cgagcggctg acgaagggcg gggtcctccc gatgatcacg agctgctcgc ccggctggat
    29581 caacttcata gaggcctttt tcccggccct cctcccccac ctctcgagct gcaagagccc
    29641 ccagcagatg ttcggcgccg tggcgaagac cttcttcgcg gagaaggcgg gcatcgcgcc
    29701 cgagagcctc cgcgtcgcct cgatcatgcc ctgcaccgcg aagaagggcg agacccgaag
    29761 gcccggcatg gacgcggcct ggcgctggtg gcgcggcaag ggccgggagc tctccgcctt
    29821 ccccgacgtc gactgggccc tcacgacccg cgagctcgcg cggatgatcc gccgcgcggg
    29881 catcgacatg gcctccctgc ccgaggagga cttcgacgac ccgctggggc ggagcacggg
    29941 cgccgcgacg atcttcggca cgacgggcgg cgtcatggag gccgccctca ggacggtcca
    30001 cgagctcgtg aacggcgcgc ccctccccgg cctggccttc gcgcccgcgc ggggcctcga
    30061 ggggatcaag gaggcctccc tcgtcctcgg agggaaggag ctcaaggtgg cggtggccca
    30121 cggcctcaag aacgcgcggg ccctcctgga gggcatcgcg gcggggacga gcccctacgc
    30181 cttcatcgag gtgatgacct gccccggcgg ctgcgtcggc ggcggcggcc agccccacgc
    30241 cttcgccgcg tcccgcggcg ccacggcgcg cggcccggtc gcgcagggcg tcgccgcgaa
    30301 gggcctttcc gcgaggaacc tcgcgatgta ccgcgaggac gagcgcctcg ggctcaggaa
    30361 gtcccacgag aacccggccg tcgcggccct ctacgcggac ttcctgggga agcccctcgg
    30421 ccacctctcc cacgagctgc tccacacgga gtacgcaagg agggagttct aggccggcgc
    30481 gcccccggcg ctcgcgctag ggcgcgggcc ggcgctcggc ttccggcgta gggcccgggc
    30541 gcgcgattcc cgcgcgcccg ggctagtccc gccaagcctc gacgacgccg cgcacttcct
    30601 tgagccgctc caggacctcc tcgcccgcgg agcagctgat ctgcgggttc gcgcgcacga
    30661 ggacctcctt cgagtccccg tcaggcgcgg ggacgtggaa gaagacggcg caggcgccgg
    30721 gcgcctcgaa gagggcgtcg cggaggtcgt agagctcgtc ctcgctcgcg atgcccggcg
    30781 ccaggcgcac gtgcacctcg cgccaggact tgtccttgag gtcggccggg tcggcgaact
    30841 cctcgacctt gaagctcggc gccgagcgcg tgaggtcgat cttccccttc aggcagagga
    30901 ccttgtcgac gacgaatcgc tcgcggaagc gctccaggac gtcggagaag acgacgatct
    30961 cgatggagcc gcggtagtcc tccacggcgc cgaaggccat cttcttgccg ttcttcgtga
    31021 ggatctcgcg gaactccttg agcatcgcga cgatggtgta gagcctctcg ggggaggagc
    31081 gctcgggacg cgagaggtcg gcctccgagc agcgctccca ggtcttccgg tactcgtcca
    31141 tcggatggcc cgagaagtag aacccgagga gctccttctc caggcggagg agctccgagc
    31201 gggggtactc ctccgcgggc tccaggacga agggcgggaa ctcctcctcg gcgcaggact
    31261 cgaagaggct cacctggccg tagcgccccg cctcctcctt cttcgccgcg tagtccgcgg
    31321 cccgctccac gttgaggacg agctcctgcc gcggccggcc gaaggagtcg aagcagcccg
    31381 agagggccag gcactcgagg gtcttcttgt tcatcccgcc cgagcccatg cgctccacga
    31441 agtccacgaa gccgcagaag ggcccgccct tcgcgcgctc ctcgacgatc gcgcgcgcga
    31501 ggccgtcgcc cacgcccttg atgccgagga agccgtagac gatcttcccc tcggagacgc
    31561 cgaagtaggc gtcggagcgg ttgacgtcgg ggggcaggag ctcgaggccc atcgaccgcg
    31621 cctcgccgat gtactcggtg agcttgtcgg tgttggcgat ctcgttcgtg aggttggccg
    31681 ccatgaactc cgcggggaag ttggccttga ggtaggcggt gcggtaggcg acgacggagt
    31741 aggcggcggc gtgggacttg ttgaa
//
LOCUS       DAOOSJ010000005        29867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1119_length_29867_cov_6.738461, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000005 DAOOSJ010000000
VERSION     DAOOSJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 29867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29867
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1119_length_29867_cov_6.738461"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLG14_00585"
     CDS             complement(<1..791)
                     /locus_tag="PLG14_00585"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX47651.1"
                     /translation="MERTDQIAAIEEAVDYIQANLDGDLTVEAIADRACFSRHYFNRL
                     FSSVTGEPVHAFVRRLRLERAAFRLVKSPGMSLTEIALEARYSPSNFAVAFRERFGMS
                     ASAYRAAPPPPPGGAYEEALARIAALRGPGAEAALAGLDAKVEVIELPELRLYRRRYR
                     GPYSELARAWEEFCSEVDRTFPDLRERDYIGISADDPLVTEGALLRYDLCVALPPGVR
                     GRAVRVPAARYACYRFDGPVSGLIHAFNDLIAIWMPRRGFRPGPGP"
     gene            complement(827..1456)
                     /locus_tag="PLG14_00590"
     CDS             complement(827..1456)
                     /locus_tag="PLG14_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47652.1"
                     /translation="MSLNRPELTDQALGILRNPSTFDWTFIPFLAFVTYVYFEEIRRR
                     NWKAVQAGLALYAVHWAYEIGNALVRHFSGHALWTVPGGTSFLLLVGVGVELSCMFAV
                     AGVIFSKLLPEDPKAKLLGVNNRVLIAVANAAFFSVFEIFLARTPAFHWVYGWWGALP
                     VFVTTYIPFFLASNFAYDWPAAKARRLVGGLSALDGLALVVFAGVLGWI"
     gene            complement(1694..2584)
                     /locus_tag="PLG14_00595"
     CDS             complement(1694..2584)
                     /locus_tag="PLG14_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47653.1"
                     /translation="MIRVGTKTLGLLVAGTIFGGVALSAGLGLWKTQGSKEPAAIRSG
                     EFAGLPSPSDIRGSYTWADVGKAFGIPPASLVRAFGAGSEAEKANSLEAKYGEVALPP
                     GTEIGTDSVRLFVSLYTGLPHEPEADTILPMTAIEILRAEAKGDRALVEAAALRAYAP
                     GAAASAGTAPAAAQAAQPAAPAPAAPAPAAPAPAAAPAAPAGTGAAAAPAASGTAPKT
                     GAGTGTAAATESHEPVAGSIVGKTTFGELKDWGFDMAAVEALLGGLGKSGESVRDYCS
                     AKGLSFSEMKTKLQELAPKK"
     gene            complement(2581..3489)
                     /locus_tag="PLG14_00600"
     CDS             complement(2581..3489)
                     /locus_tag="PLG14_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX47654.1"
                     /translation="MPAKIKAPRPKAQRWRRGIQIFFFVLVALVATTSALKEKGVVLP
                     LAGSASLHAICPFGGVVSFWQLATAGTLVKKVHESSVVLAVLGLLLALLFGPVICGWI
                     CPFGSFQEWLGKLGKRIFGKRYNTMVPRGLDRILRFLRYAVLVWVSYMTVVTGKLFFQ
                     DYDPYFALFNFWTGEVAVAGFVALGATVLLSLAVERPFCKYACPYGALLGIFNLFRIF
                     GIKRKASSCISCHACTKACPMNIEVESLTAVRDHQCITCLKCTSEAACPVPSTVELAS
                     GALEPAGAKKPVAEPSAKAAAEGGSL"
     gene            complement(3548..4084)
                     /locus_tag="PLG14_00605"
     CDS             complement(3548..4084)
                     /locus_tag="PLG14_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47655.1"
                     /translation="MNVMRNHPSRLAFVLAAALVASPSFGQAAGSAAWGAKAAAADPG
                     ASVESMLRYAIEDEYLARAEYVAIMRRFGEARPFSNIKASEDSHIAWLEAEYGARGLP
                     VPKDEGAAHAFVPASLKEAYAAGVQAELDNIAMYDSFLRSPALSGAADAALRALFSRL
                     KAASENHLAAFRNGLSKY"
     gene            complement(4646..5035)
                     /locus_tag="PLG14_00610"
     CDS             complement(4646..5035)
                     /locus_tag="PLG14_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOX47656.1"
                     /translation="MTRSRAAALAVLEAAASPLRASDVLDAIGGACDQATVYRSLHFL
                     EEGGWAESFVLHCSEHGTERYFVSAGKPHRHFFHCESCHRFIDLGACRLGGLVAELER
                     EYGLEVKSHTLYFSGLCAECRAKGGGA"
     gene            complement(5032..5934)
                     /locus_tag="PLG14_00615"
     CDS             complement(5032..5934)
                     /locus_tag="PLG14_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOX47657.1"
                     /translation="MNGFLGALLDPSLPFIRNALLAGLLSSVLFGVLGSVVTVKRIAG
                     LAGAVSHAVLGGVGLALFLSARGLVPGLPPIAGALAFALLAALLIALVSLKAKQREDT
                     VINAIWAIGMSLGVLFIAKTPGYIDPMSYLFGNILLVSGRDLVLLAAMDVVAVAGAFL
                     FYPQLEASAFDEEFAKTRGLRTDLVFLAVLAAVAVAVVLLQTFVGIVMVIAMLTLPAG
                     TAGFGAKNLAGMMAGATLLSVLFSVGGLAASWSLDLPAGATVVVLAGAAFLLVSGGRV
                     ALLALRRARAERERRGMTAGDSGR"
     gene            complement(5931..6713)
                     /locus_tag="PLG14_00620"
     CDS             complement(5931..6713)
                     /locus_tag="PLG14_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX47658.1"
                     /translation="MPRDLAVRFDSVRFSYGSIPVLEDASFHIHEGEFAALLGPNGSG
                     KTTLLKLLLGLARPDSGRILVFGRPAEEARKAIGYVPQAVVYDPAFPISVAEVVRMGT
                     LRGSRGRYRPEDAEAALRAMAAAGVAELASRPYSALSGGQRRRVLVARALAAEPRLLV
                     LDEPTSNMDAESEALLYASLGALKGRATILIVTHDTGFVSALTDSVLCVGERSGKRGS
                     VVRHPSVPAEGVLAPETYGGAALRVLHDAELPDDGCCREAGR"
     gene            complement(6715..7656)
                     /locus_tag="PLG14_00625"
     CDS             complement(6715..7656)
                     /locus_tag="PLG14_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOX47659.1"
                     /translation="MQFRRLALAASSILFAAGLGFAAGPGVAGSAPAAKKLVVAVSVL
                     PQAYFVERVGGDRVRAFALVGPGQSPHSYEPSPRQMAELGQASLWLTVGIEFEHGLEP
                     KVARLYPALRIRDNAAGVVFRRLEEHHHEEGEQHEEGEEESGGHDEGEDGRDPHAWLG
                     RQGSKAMAAEIRDALSLLDPSGAAAYARNYEALARDIDAVFAGLVAKLGSLKGRPVFV
                     YHPAFGYFLDEFGIEQVAVETGGKEPTQKGLSALVARAKAEKAAAVFVQAQFPTAAAR
                     SLASSIGAAVVPIDDLAPDWLSNLARMGEALLKAARP"
     gene            complement(7805..9271)
                     /locus_tag="PLG14_00630"
     CDS             complement(7805..9271)
                     /locus_tag="PLG14_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX47660.1"
                     /translation="MTIFLLASLLCLSLPRPGLGAQEASFRYAATGRYKLVERSDFSK
                     YVDGAYLGHVYRETRALLDGEPEAGGVRYRGGFLVFEETLRDAALVSRRVEEEIPADF
                     LVASDGGLELLRDEGYPSLRGFPAFPAGPVAPGESWQAEGVRVADPENSGRPTRIPIL
                     AEYVYRGLSSYKGREAHSISAKFATRYRSGQDRQGDPELRSATGTHDATILLDAETGA
                     PLLIRDSLDETFVYASGRSVRLKGFLLVFYDATLALDYGAATASLRRALSGGGPGPAT
                     AASAGAGVAPSGGAAAAAGPGSASGTAGSDGASDGAAAAAGPASDVKPGAAPAGGEGG
                     PGAGTVSGPASSGAGPGAFLVEGGPELDLGAGVSAVKAPEGLLLTVKDLRFVADGDAV
                     LPEERWRLDAIADALKGIPGRSFLVGGHTASVGRPADEMALSIRRAKRVVDELAARGI
                     PAGRLLYRGYGATKPLVPNDSEANRARNRRVEILVLED"
     gene            complement(9292..9759)
                     /locus_tag="PLG14_00635"
     CDS             complement(9292..9759)
                     /locus_tag="PLG14_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOX47661.1"
                     /translation="MRIAVITVSDRASAGVYADRSGPAVVETLKSALPGAEIEAEIVP
                     DGEESVLAALSRHPEADWILTTGGTGPAPRDRTPEATRAYCDRSMPGIADYLRSRSLE
                     ETPNAVFSRGEAGMRGLQYVVNFPGSEKAARFCAAALAPLMEHGRKMARGEGH"
     gene            complement(9728..10225)
                     /locus_tag="PLG14_00640"
     CDS             complement(9728..10225)
                     /locus_tag="PLG14_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOX47662.1"
                     /translation="MGALPREGGFEIVSLNVSERTGTRKRPVASFTLAEGKGVAGDAH
                     AGLAEDRQVSLLAAEEIEAASAVLKAGGPSCAKAGLDGLKPGDFAENVTTRGLALHEL
                     PIGTRIEMGEAVLEVSKIGKECHAACEIRRLVGDCVMPRKGIFMRVLKGGEVRVADRG
                     HYRIG"
     gene            complement(10225..10680)
                     /gene="moaC"
                     /locus_tag="PLG14_00645"
     CDS             complement(10225..10680)
                     /gene="moaC"
                     /locus_tag="PLG14_00645"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOX47663.1"
                     /translation="MGSELTHVDGAGKASMVDVSGKGRVRRTARAAGRIDLAPGTVAL
                     VRANALAKGDVLAVARVAGITAAKRTSELIPLCHNVEIEHVEVRLDIDDGGISIEAKS
                     VCTDKTGIEMEALTAVSVAALTVYDMCKAVDKNMRIGGIRLLEKTKEAS"
     gene            complement(10683..11480)
                     /locus_tag="PLG14_00650"
     CDS             complement(10683..11480)
                     /locus_tag="PLG14_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX47664.1"
                     /translation="MLDSYGREIGYLRISVTDRCNLRCVYCMPEEGVRLLRHEDILSF
                     ERITLVAAAAAGLGFGKIRLTGGEPLARKGIERLVSMLAPLPGVRKLAMTTNGTLLAP
                     LAGELKARGLSSVNVSLDTLDPVRYRALTRGGELSEALAGVEAARTAGLPVKLNVVAL
                     EDSTEEDFGELRAFAASAGAEIQFIARYRLDAEKRDGGDYDRPPKCGSCDRLRLLADG
                     TLRPCLHGNVSVPVDFSDIEGSLRRAVFAKPERGHVCVGLEVGQIGG"
     gene            complement(11458..12855)
                     /locus_tag="PLG14_00655"
     CDS             complement(11458..12855)
                     /locus_tag="PLG14_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOX47665.1"
                     /translation="MPQEPIHPDEALRLVLEAAPPPELETVALSEALGRALPRAVLSA
                     IDHPPFDKSAMDGFAYARAGSGAAAAGCACCGGAPVASSVGAGGEGPAAGAGAAAAGG
                     AFHVVELVAAGAAPTRALAPGECARIMTGAPLPSGADSVQRIEWTESAGKDAAGADFV
                     RFTRPESVSNVIRRGENLKAGGELLGPRLLAPQDLGILASGGVAELQVARRPLVGVVS
                     TGDELAAPGSPLGPASIYDSNGPQLAAQARAAGCEARFYGAFPDEPGALAAVLGRALA
                     ECDVVLVSGGVSMGDFDYVPRSLEALGVRRVFHNLAMRPGKPTYFGTLGAKAAFGLPG
                     NPVSTFVNFEVLVRPHLERRMGLEYRPRVVRARLAAPLSRRGSDRVEFLPARLERAPL
                     GSGAADAAGAGGRESASGRESAREGACELARPLGYHGSSMLNVLAEADALLRMEIGVD
                     RIEEGAWVDARLLRP"
     gene            complement(12866..13603)
                     /locus_tag="PLG14_00660"
     CDS             complement(12866..13603)
                     /locus_tag="PLG14_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOX47666.1"
                     /translation="MSGAPLFLARDLELRYGPEPRRSQGLARHLRQRGPREPGAGAVG
                     LRRWAAFAAQRLEVAEGECLALLGPNGSGKTTLLKALGGLLKPAAGSLLFLGEEASSS
                     RSLRRRGVYLHQAPYILSGSVSYNLYYGARARGLPRGAADDGVREALSLLGLSGFERR
                     GHRELSGGEAQRVALARAFAAGADVLLLDEPTASADAASARLVLEALRARRAAGATIV
                     FSTHDPKLAEALATRTLLLEDGRAALS"
     gene            complement(13600..14274)
                     /locus_tag="PLG14_00665"
     CDS             complement(13600..14274)
                     /locus_tag="PLG14_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX47667.1"
                     /translation="MREAFLLLVSGDAETWGIVGRSLRFSFFSTLFALVPGIPAGVAL
                     GTRAFRGRRSLVALLNALMALPTVVIGLLVYSLISRSGPLGFLDLMYSPGGVVLGQAF
                     LAFPVVASLTQAGLSKLDPRFRETILSLGAGPLRRLASTLFEARAVLVAAVVTAFGRV
                     TGEVGVSMMLGGNIRFSTRTMTTAIALDAAKGEFERAVSLGLVLLAIALGVNLCLHAL
                     SPREAS"
     gene            complement(14284..15102)
                     /locus_tag="PLG14_00670"
     CDS             complement(14284..15102)
                     /locus_tag="PLG14_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX47668.1"
                     /translation="MKRLSALVLALALSGLAFAQAPAAVPANPRLKLATTTSTEQSGL
                     LAYMLPVFEKESGYKVEVIAVGTGASLKIGEKGDCDVVLVHARALEDAFMAAGFGADR
                     KDVMYNDFIVLGPASDPAGLAKAKSATEALSLIAKSGASFVSRGDKSGTHVKELEVWK
                     AAGVSPVGAAWYKEAGQGMSQVIVMADQMQGYTIADRATWLAMKDKSKLRILSQGDPV
                     LFNPYGVITVNPAKWPTANYAGAKALMEFMTGPKGRELISAYKISGEQCFYLYK"
     regulatory      complement(15093..15261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     assembly_gap    15265..15364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15551..15982)
                     /locus_tag="PLG14_00675"
     CDS             complement(15551..15982)
                     /locus_tag="PLG14_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOX47669.1"
                     /translation="MLKETRTTRLVKSQDLNHHGTLFAGRMAEWFTESCFLAAARFVG
                     RPEDLVCAMIHGLRFTKPARPGDAIELVARVARCGSKSLTIGAEVFVNDEAEPTIRGF
                     ATFVTVDAEGRPYAHGLSLPPEWTAANRGLCEEAGRLAASR"
     gene            16158..16790
                     /gene="msrA"
                     /locus_tag="PLG14_00680"
     CDS             16158..16790
                     /gene="msrA"
                     /locus_tag="PLG14_00680"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOX47670.1"
                     /translation="MVKNLAMAMAAAIALLACAAGEPGSGGAAAPRGKETDAMQAEGK
                     LEYAILGGGCFWCLEAAYELLPGVVSVENGYAGGSRRNPSYEQVSSGATGHAEVVRIV
                     YDPAKVSYEELLDLFWKIHDPTTEDRQGADIGSQYRSVIFYAGEEQRLAAEASMRETQ
                     RRLVDPIVTELLPVPDFWIAEDYHQDYFRRNPEAAYCRIVIAPKLKKAGF"
     gene            16969..17607
                     /locus_tag="PLG14_00685"
     CDS             16969..17607
                     /locus_tag="PLG14_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX47671.1"
                     /translation="MAVGVEGDKGTAERILEAAGELLASGGYEALSMRKIAAMVGLSQ
                     AAIYRHFKDKDELVGRLVASGYAELVALVESLEADGGPPDQVLERNIEAYVGWALGRP
                     TVFKALLLREIGPAAEAVNSLSEGIARRRRTFALLAARLRRGMDEGVFEPADPELTAQ
                     AVWTAMFGLASRLVLEKDLPPDRAALLTARLSRIVIQGLRPLGGEPNNKEGA"
     gene            17604..18092
                     /locus_tag="PLG14_00690"
     CDS             17604..18092
                     /locus_tag="PLG14_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOX47672.1"
                     /translation="MKALVIYDTKHGSSAEAASAVARGIGPGAEARRLSEAAGSLEPY
                     DLVVVGGPVYFGKWSESAGEWLAARAPELEGKRLAVFALGSMPELSAERIRATLPPAL
                     RARLAAAVDFGGRIPAEGLSFIERTLTRLIAKANAKSGAATRELDLAEAEAFGASLAA
                     GK"
     gene            18104..18574
                     /locus_tag="PLG14_00695"
     CDS             18104..18574
                     /locus_tag="PLG14_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOX47673.1"
                     /translation="MTDMNKRGQRVEGREAAQARAAKAPALRAPVLRAAFAALALALL
                     SSCATPSIPLAVTPPLSALKDGRYEGSYDAKMVKATVAVLVKDGKIAEVTILSHDSSP
                     IGKKGEAVAARVVAAQSLEVEAVAGATGSSRAILKAIENALAGAGPDAASGATE"
     gene            18571..19002
                     /locus_tag="PLG14_00700"
     CDS             18571..19002
                     /locus_tag="PLG14_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOX47674.1"
                     /translation="MSRASRVVLAVLCALVLLLALGFVAVFFSFGPGRDSGVRPLGPA
                     ALADGSYRGSGSAGAPFGAIAAFRKVEVELEVRDGRIESLRYLKPETEGPAAEVFAEL
                     SRRVLERQGPDIDAVSGGTWTSRALRKAVESALERGGAKPR"
     gene            complement(19171..19605)
                     /locus_tag="PLG14_00705"
     CDS             complement(19171..19605)
                     /locus_tag="PLG14_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOX47675.1"
                     /translation="MMIRKFSKDFFEPGPLAFLGYSSKSPAYSAEIKEALEGGGFAVH
                     AVNKRPSAKLEAPVHSSLGELPPETRQAFVLLAGGRALEAVEELGKLGYKKVLFRSRG
                     LAGQAALERAAALGMEVAAGCPLMAYGKGLHRFHGFLAGVRG"
     gene            complement(19661..20689)
                     /locus_tag="PLG14_00710"
     CDS             complement(19661..20689)
                     /locus_tag="PLG14_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX47676.1"
                     /translation="MKLRVKGQAALYLAATILALVSLCVKLASRYYSPLFVSASRFLL
                     GAVFCVVTLSLGFPGTKPKRIGAVILRGFFGSLAMITSYAAVSLTGPGRATLLANTYP
                     LFVAFFGALLFGEKVGARTFASALVCAGGAFLVVRDGSGAALSGDLLALASAVFSGFA
                     VNFVRRASAAGENPFVLYLSPCLFGLPLLAFAPGPGPLIHSGAAGLLLLFAVGFGGFA
                     AQAILSYGYRTVPASRGSVVFYWETALTVLLGLLFGGERLNLRFALGLAAIVGGLWLN
                     RPSAAQASGGAAQAAQPGDDAGAQGSATQAAQPGDDAGAQGSAAQAGSASAEVGAGEP
                     LAPPDGKR"
     gene            20832..21359
                     /locus_tag="PLG14_00715"
     CDS             20832..21359
                     /locus_tag="PLG14_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47677.1"
                     /translation="MGDVTRRMLKLGAFAALFSLFHFAYDWWPSAITAVFSGVDESVF
                     QHMKTGYFAWLAASALESLAARPPRGLRLGFAAVRLWGAFLVVYLFVSLWYLGPALVG
                     PMPSDAAELALAFSSVALGGLAALGLEAQLGKAPLSRGLELGAMAATLLALYLFARFT
                     ATRPWIDLFELPPPH"
     gene            21588..22061
                     /locus_tag="PLG14_00720"
     CDS             21588..22061
                     /locus_tag="PLG14_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47678.1"
                     /translation="MSDTVIVYVTRDGYSRALAEELGRLVGAPAAEIGDEVKRRGFFG
                     YMRSGFQAAARKATPIRDPGVELSRAKRVVLVQPVWASAVCPPLRTWLRAHRPELAGK
                     RIGLVASEKGSPPEKLKLAYETEFGDLAAFACLRESEAGAAKSAALAAFARELGA"
     gene            complement(22171..22890)
                     /locus_tag="PLG14_00725"
     CDS             complement(22171..22890)
                     /locus_tag="PLG14_00725"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOX47679.1"
                     /translation="MPYCSRCGVEVDEGVPACPLCRAPIQRLDEGEARPGLKPWPEHV
                     VPDELPPLSAKERLKFAVEIAAVSMGIALAMLLLIDLLANRRVTWSAYPATGLVFGWL
                     CLAVPFFLGRRPWLVLAALGPAAFLLLFLLDAIDGSIEWFPSYGLPIAAIALAAVASV
                     ALASALAKKRGLNVVGFAFLGAAFVCTGIDLTVGLNRGRGLAMRWSVPVDFALVPIAA
                     FLIYLHYRVTKKVDMRKAFHL"
     gene            complement(22913..24232)
                     /locus_tag="PLG14_00730"
     CDS             complement(22913..24232)
                     /locus_tag="PLG14_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47680.1"
                     /translation="MATEKTEAPRGADQRRRRHWFKLDNAAILFPTFASGTISTLFRL
                     SMTLDEPIRLSTLEAALERVHPCFPYFNVELHAGAFWYYFETISRPPRVMADSKYPCM
                     NLRLRDRGRYLFRVRAYDRRVAVEFSHILTDGTGASIFLKSLVAEYLRLRGALVAEDP
                     TVFRPGRVPEDEEFEDAFKRYCDPSLPWPEKEKVAFHIPARLERRGVYHVVGASMPLA
                     PLLALARERRVSLTEFLAAVQVAALQDLWEALPERSRRRKPALLKLMVPINLRKPFPS
                     RTMRNFSLFALPGVDRRLGHFEFEEIVQEVHHSMRLQGTPREVARQITRNVTSTRNAF
                     VRAMPLFLKILGGRALYKRFGELLYSSCLTNLGALELPEGMRERVTRADFVSAPAPLT
                     GTNCAMLSLGDRVYLSFARLARESELERLFFTRLRRMGIPVKLESND"
     assembly_gap    24482..24581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24582..25957)
                     /locus_tag="PLG14_00735"
     CDS             complement(<24582..25957)
                     /locus_tag="PLG14_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47681.1"
                     /translation="MRARSSMAALAALLLVLAAAAPALAEAPALDLAASGWLAASAYA
                     RPNAEEPFAAAGLRGGLALDGYAFDAVGLRLSLEAYTNLAGLAYAVDWSLARPDPLSL
                     AATGNGRLMAALDLKEAWLEFSSGALDARLGKQVVAWGLADGNNPTDNVNARHVGTRF
                     VSTLDEQKLGALAANLVYNLPGNLGTVQGLFLPVSVPNDMSSIAQTITAGPTTIVIED
                     DEAPGLAIVNFEGGLRALFYLGSVSASASWLSYLNRFPDYEVTVVGGPAPLITLSPFH
                     GRVNQFGLDAAWLPGGFDLRTEWALSLTGDEDGTDLGLANSSLSGVVQASKSFFDSRL
                     TASLAWAPRIVFDHHEASDYASPGDQMLAAELAKYNGQAYAFEQVGSLRLAGKLLGET
                     LQPEALFLASLEARDFLATASVAYNVADGVNLKGGLGIYGSFREEGDPEREWGTFSNS
                     RTIDNDYL"
     gene            complement(25957..26751)
                     /locus_tag="PLG14_00740"
     CDS             complement(25957..26751)
                     /locus_tag="PLG14_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX47682.1"
                     /translation="MKRTLMAAAFAAALAAVLPAQDLESLLRRNETQPRPPATEGEMK
                     MVITNKAGQSRVREIKAYTKTEADGTDRQLLVFLAPADVRDTRFLTVDYEAGGKEDEQ
                     YIYIPALRKVRSIGTSGGDSKTGAFLGSDFSFADIGTLDRADFAVRALGEDTIEGRRY
                     LKVEYASKGPATLKKYGYGKVVRWIDAENATSRQSEYYDAGGKLVKRLVVRGQRLVDG
                     KYWQFESMEMSNLETGGKTEWVFVKSVNLPSIDDGYFTLRFLERGR"
     gene            complement(26798..29719)
                     /locus_tag="PLG14_00745"
     CDS             complement(26798..29719)
                     /locus_tag="PLG14_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOX47683.1"
                     /translation="MRISFKGLSRFIASRPKTVIALVLAVTLGLGFGLAGMRVDSDMT
                     DDIPDTVPEKAFYDEVGKIFPSDDFLIVALTDSSGAFSPGMLRQVREWSGALEAVEGV
                     KGVISISSAGLIKGTEEGLVIEEAMPVLPETAEEIAAFRAKIEASDMTKSLVGKDGKS
                     TAILVTMKEGVDAEALPRVRITLPKGASADEAFLAALSGVSAAAKEGEAPSPAVARVY
                     LPGSEADRAAAKARYGESLGPDKKGRTTVLAALAPGLPLEPAASSVAELAAAKGWKAK
                     AAAAPFSGYERIMATIEGLPSYVDGKVYVSGAKAVSTIVGKLLIKDLALLFPVVIAVI
                     ALILFLSFRTARGVFMPLGNVVLSVIWAMGLMGLIGQPITMATMILPIILIAVGTAYT
                     IHVINRFYEDLVAIEDKEKAVESTVTHVALPVFLAGATTVIGFGSLAFSSLAALRMFG
                     VLSALGILFALVLSLTLTPALLRLMPKPKARTVAAHDDSRLASALAASGRFVAKKPVL
                     VLAASLALVAGFGAFAPRVSFETNTLNSFKAGSEIRRASEYLNENFTGITVMTVIVKT
                     DEEGAILDPAVLRAMDGLQKELESLRSVGKKVVGPEDPRYGEGKELVGGSQSIVTFVK
                     GINKALNEDDPAFDKVPDDVTPVPVATEVYALKGGVLEERDSETRELLAAYGPADGLR
                     VEGGFAFVAAGGAERKVDLATGRAVELVPGRDYAGQLVFQYENSGKPENIEGFIDNPR
                     KTARVNVFLKTASSGLIGQVQARARSYIAAEFPAGTRADITGLSNLTLAILRLLVSSQ
                     VSSVLASLVVCFLFISLISRSFVEGLFSIVPLTASLIINFGVMAAFGIPIDISTATIA
                     SIGIGIGIDYTLHFLERLKLSLCGMELEAAIEETMRTTGKGIFVNALAVAGGFAALLF
                     SQLRGNVFMGLLMALIMMTSSVFAVTLLPALLVLVRPKFVGKKALASRCGDERADGPS
                     L"
BASE COUNT         4458 a         9991 c        11174 g         4044 t
ORIGIN      
        1 ggccccgggc cgggcctgaa gccgcgccgc ggcatccaga tcgcgatgag gtcgttgaag
       61 gcgtggatga gcccggacac gggcccgtcg aacctatagc acgcataacg cgcggcaggc
      121 acgcggaccg cgcggccgcg gacgccgggc ggaagggcca cgcagaggtc gtagcggagg
      181 agggcccctt ccgtgacgag cgggtcgtcg gcggagatgc cgatgtagtc gcgctcgcgc
      241 aggtccggga aggtccggtc gacctcggag cagaattcct cccaggctcg ggcgagctcc
      301 gaataggggc cgcggtagcg ccttcggtag aggcggagct cgggcagctc tatgacctcg
      361 accttcgcgt cgaggccggc gagcgccgcc tcggcgcccg gcccgcgcag ggccgcgatg
      421 cgggccagcg cctcctcgta ggccccgccg ggcggcggcg gcggcgcggc gcggtacgcg
      481 ctggcgctca tcccgaagcg ctccctgaag gcgaccgcga agttggatgg gctgtagcgc
      541 gcctcgagcg cgatctcggt gaggctcatt cccgggctct tcacgagccg gaaggccgcc
      601 cgctcgagcc tgaggcgccg gacgaaggcg tggacgggct cgccggtgac cgaggagaag
      661 agcctgttga agtaatgccg cgagaagcag gcccgatcgg cgatcgcctc gacggtgaga
      721 tccccgtcga ggttcgcctg gatgtagtcg acggcctcct cgatcgccgc gatctggtcg
      781 gtccgttcca tcgcctaact ccgccgccgc gataacgcgc cgcggatcaa atccagccca
      841 ggacgccggc gaagacgacg agggccaggc cgtcgagcgc ggagaggccg ccgacgaggc
      901 gcctcgcctt cgcggccggc cagtcgtagg cgaagttaga agccaggaag aagggaatgt
      961 aggtggtgac gaagacgggc agcgcgcccc accagccgta gacccagtgg aaggccggcg
     1021 tcctcgccag gaagatctcg aagaccgaga agaaggccgc gttcgcgacc gcgatgagga
     1081 cgcggttgtt cactcccagg agcttcgcct tcgggtcttc ggggaggagc ttcgagaaga
     1141 tgacgcccgc gacggcgaac atgcaggaga gctccacgcc taccccgacg aggaggagga
     1201 agctcgtgcc tccgggcacg gtccagaggg cgtgcccgga gaaatgccgg acaagcgcgt
     1261 tgccgatctc gtaggcccaa tgcacggcgt agagggccag gccggcctgc accgccttcc
     1321 agttcctgcg cctgatctcc tcgaagtaga cgtaggtcac gaaggccagg aaggggatga
     1381 aggtccagtc gaaggtggaa gggttccgca ggatgccgag cgcctggtcg gtgagctcgg
     1441 gacggttcag gctcatgtcg ccccctgttc aggagaggat cgccgtccaa ggagcgacgc
     1501 gcgccccgga cgacgttgtg ctcccgatgc tacacccgcc aaggcgcggg cgcaacgagg
     1561 atcccgcggc cggacctggg ccgggccaat cgcggcccgg ccccccatcc gcgagggctg
     1621 cccgcggccg ggtcccgcgc ggccgggcca atcgcggccc ggccctcccc ggtcggagat
     1681 tcttgagccc gcctcacttc ttcggggcga gctcctgcag tttcgtcttc atctcggaga
     1741 aggagagtcc cttcgccgag cagtagtcgc gcaccgactc gccggacttc ccgaggccgc
     1801 ccaacagggc ctcgaccgcc gccatgtcga agccccagtc cttgagctcg ccgaaggtcg
     1861 tcttgccgac gatgctgccc gcgacgggct catgggactc cgtggccgcg gccgtgcccg
     1921 tgccggcgcc tgtcttgggc gcggttccgg acgcggcggg agcggctgcg gcccccgtcc
     1981 ccgcgggagc ggccggcgcc gcggccgggg cgggggcggc aggagccggc gcggcaggcg
     2041 cgggcgcggc gggctgcgcc gcctgcgctg ccgccggagc cgtgcccgcg gaagccgccg
     2101 ctccgggcgc gtaggccctc agggccgccg cctcgacgag ggcgcggtcc cccttcgcct
     2161 cggcgcgcag gatctcgatc gcggtcatgg gcaggatcgt gtccgcctcg ggctcgtggg
     2221 gaaggcccgt gtacagggac acgaagagcc gcaccgagtc ggtgccgatc tcggtgcccg
     2281 gaggcagggc gacttcgccg tatttcgcct ccagggagtt ggccttctcc gcctccgagc
     2341 cggcgccgaa ggcgcgcacg aggctcgccg ggggaatgcc gaaggccttg cccacgtcgg
     2401 cccaggtgta ggagccgcgg atgtcggagg ggctcgggag gcccgcgaac tcgcccgacc
     2461 tgatggcggc gggctccttg gagccctggg tcttccagag gccgaggcct gcggagaggg
     2521 cgacgccgcc gaagatcgtg cccgcgacga ggagtcccag ggtcttggtg ccgacgcgga
     2581 tcatagcgag cctccttcgg cggcggcctt cgcggagggt tccgctaccg gcttcttggc
     2641 gcccgcgggc tccagggcgc ccgacgcgag ctccacggtg gaaggcacgg ggcaggccgc
     2701 ctcgctcgtg catttgagac aggttatgca ctggtggtcg cgcacggccg tcagggactc
     2761 gacctcgatg ttcatggggc aggccttcgt gcaggcgtgg caggagatgc agctggaggc
     2821 cttgcgcttg atcccgaaga tcctgaagag gttgaagatc ccgaggaggg cgccgtaggg
     2881 gcaggcgtac ttgcagaagg gccgctccac cgcgagggag aggaggaccg tggcccccag
     2941 ggcgacgaag ccggccacgg ccacctcgcc cgtccagaag ttgaagaggg cgaagtaggg
     3001 gtcgtagtcc tggaagaaga gcttgcccgt gacgaccgtc atgtagctca cccagacgag
     3061 gacggcgtag cggaggaagc gcaggatccg gtccaggccc ctcgggacca tcgtattgta
     3121 gcgcttcccg aaaatccgct tcccgagctt gccgagccat tcctggaagg agccgaaggg
     3181 gcagatccag ccgcagatca cgggcccgaa aaggagggcg aggaggaggc cgaggacggc
     3241 gaggacgacg gaggattcgt ggaccttctt cacgagggtg ccggccgtcg cgagttgcca
     3301 gaaggagact acgccgccga aggggcatat cgcgtggagc gaggcggagc ccgcgagggg
     3361 caggaccacg cccttctcct tgagggcgct ggtcgtcgcg acgagggcga cgaggacgaa
     3421 gaagaagatc tggattcccc ggcgccatcg ttgcgccttg ggcctcggag ccttgatctt
     3481 tgcgggcatg gttcctcctt gacgacttaa cgttttgggc ctggcgcctc cgccggcccc
     3541 gcgggtctta gtacttcgag agaccgttcc tgaaggcggc caggtggttc tccgaggcgg
     3601 ccttgagccg cgagaagagc gcccgcaagg cggcgtctgc cgcgccggag agcgcggggc
     3661 tccgcaggaa ggaatcgtac atcgcgatgt tgtccagctc cgcctggacg cccgcggcgt
     3721 acgcttcctt gagcgaggcg gggacgaagg cgtgggcggc gccctcgtcc ttggggacgg
     3781 gaaggcccct ggccccgtac tcggcctcga gccaggctat gtgggaatcc tcggaggcct
     3841 tgatgttcga gaagggacgg gcttctccga atcgtctcat gatggccacg tactccgcgc
     3901 gggccaggta ctcgtcctcg atggcgtagc gcagcatcga ctccaccgag gcgccgggat
     3961 cggcggccgc ggccttggcg ccccaggcag ctgagcccgc cgcctgcccg aagctcgggg
     4021 aggcgacgag ggcggcggcc aagacaaagg cgagtcggga aggatggttt cgcatgacgt
     4081 tcatatcgtt gcctcgtttg aagcgggatg acatacatat agcaatatat atgccaaata
     4141 ctgcaactgc gcgctacatt gcgcgctttt tctacgagtc cggcgcctcc gcaggccgtg
     4201 ccaagccgtc gccacaaacc atctctttac tacaaggcaa tttgcgtatg ccgatccagg
     4261 cttggccttg atgccgcccg tcgcccttcg cccgactccg gcctcaaggc caccccgccg
     4321 cgcgcctact gaagcataag cgaagcgagg ccccttgcca aacgcaggca agggaaagag
     4381 aagcgaggag ctctgtgggc attttttgcc cagccgcggg aaagtactgc ccgcggccgc
     4441 gcggcctgtc tcggcacgac gtggtcggcc attgcgccgc ccggcctgca gctctcgacg
     4501 gcgctgccag ccgcgacccc acggagctcc ggcgcgatcc ggagcggcgg cgatgccgcg
     4561 gcgcggtgcg gcgcggttgt cggccgcggc accgcgcggc ggcgcacgct agagagtccc
     4621 gccgaatgcg gcgagaggct aggcgctagg cgccgccgcc cttggcgcgg cactcggcgc
     4681 agaggccgga gaagtagagc gtgtggctct tcacctcgag gccgtactcg cgctcgagct
     4741 cggcgacgag gcccccgagc ctgcaggctc cgaggtcgat gaagcggtgg caggactcgc
     4801 agtggaagaa gtggcggtgc ggcttcccgg cggagacgaa gtagcgctcg gtgccgtgct
     4861 ccgagcagtg gaggacgaaa gactcggccc agccgccctc ctccaggaag tggagggagc
     4921 ggtacaccgt cgcctggtcg caggccccgc cgatggcgtc gaggacgtcg gaggcgcgca
     4981 gcggcgaggc ggccgcctcc aggacggcga gggccgcagc cctggagcgg gtcatcggcc
     5041 tgagtccccg gcagtcatcc cgcggcgctc ccgctcggcc ctcgcgcggc gcagggccag
     5101 gagggccacc ctgccgcccg agacgaggag gaaggccgcg ccggcgagga cgacgacggt
     5161 ggcgccggcg ggcaggtcca gggaccagga ggccgcgagg ccgccgaccg agaagaggac
     5221 ggagaggagg gtcgccccgg ccatcatgcc cgccaggttc ttcgccccga agcccgccgt
     5281 gccggcgggc agggtgagca tcgcgatcac catcacgatg ccgacgaagg tctggaggag
     5341 gactacggcc acggcgaccg ccgcgaggac ggcgaggaag acgaggtccg tgcgcaggcc
     5401 cctcgtcttg gcgaattcct cgtcgaaggc ggaggcctcg agctggggat agaagaggaa
     5461 ggctcccgcg acggcgacga cgtccatggc cgcgagaagg acgaggtccc gcccggagac
     5521 gaggaggatg ttgccgaaga ggtagctcat cgggtcgatg tagccgggcg tcttcgcgat
     5581 gaagaggacg cccaggctca tcccgatggc ccagatcgcg ttgatgaccg tgtcctcccg
     5641 ctgcttggcc ttgagggaca cgagggcgat gaggagggcc gcgaggaggg cgaaggccag
     5701 ggctcccgcg atgggcggca agccgggcac taggccgcgg gcggagagga agagggccag
     5761 gcccacgccg cccaggaccg cgtgggacac ggcccccgcg aggcccgcga tgcgcttcac
     5821 cgtgacgacg gagccgagga cgccgaagag gactgaggag aggaggcccg cgagcagggc
     5881 gttcctgatg aagggcaggg aggggtcgag gagggcgccg agaaagccgt tcatcgccca
     5941 gcctcccggc agcagccgtc gtcggggagc tcggcgtcgt ggaggacgcg gagggcggcg
     6001 ccgccgtagg tctcgggggc caggacgccc tccgcgggga cggaggggtg gcggacgacc
     6061 gagccccgtt tccccgagcg ctcgccgacg cagaggaccg agtctgtgag ggcggagacg
     6121 aagcccgtgt cgtgggtgac gatgaggatg gtcgccctgc ccttgagggc gcccaggctc
     6181 gcgtagagga gggcctcgct ctcggcgtcc atgttggatg tgggctcgtc caggacgagg
     6241 agccggggct cggccgcgag ggcgcgggcg acgaggacgc ggcggcgctg gccgccggag
     6301 agggccgagt agggccggga cgcgagctcg gcgacgcccg ccgccgccat cgccctcagg
     6361 gcggcctccg cgtcctcggg gcggtagcgg ccccgcgagc cgcgcagggt gcccatgcgc
     6421 acgacctcgg cgacggagat cgggaaggcg gggtcgtaga ccacggcctg gggcacgtag
     6481 ccgatggcct tcctggcctc ctccgccggg cggccgaaga ccaggatcct gcctgagtcc
     6541 ggcctggcga ggccgaggag gagcttgagg agggtcgtct tccccgagcc gttgggcccg
     6601 aggagggcgg cgaactcccc ctcgtggatg tggaagctcg cgtcctccag gacggggatg
     6661 ctcccgtagg agaagcgcac cgagtcgaag cgcacggcca ggtcgcgcgg catcctaggg
     6721 cctcgccgcc ttgagcaggg cctcgcccat gcgagcgagg ttcgaaagcc agtcgggggc
     6781 caggtcgtcg atcggcacga cggccgcgcc gatggaggag gcgaggctcc gcgcggcggc
     6841 cgtcgggaac tgggcctgga cgaagacggc ggcggccttc tccgccttgg cccgggcgac
     6901 gagggcggag aggcccttct gcgtcggctc cttgccgccg gtctccacgg cgacctgctc
     6961 gatgccgaac tcgtccagga agtagccgaa ggccggatgg tagacgaaga ccggtcggcc
     7021 cttgagcgag cccagcttgg ccacgagccc ggcgaagacg gcgtcgatgt cccgtgcgag
     7081 ggcctcgtag ttccgcgcgt aggccgcggc gcccgaggga tcgaggaggg agagggcgtc
     7141 gcggatctcc gccgccatcg ccttggagcc ctgccgccca agccaggcgt ggggatcgcg
     7201 cccgtcctcg ccctcgtcgt ggccgccgct ttcctcctcg ccctcctcgt gctgctcgcc
     7261 ctcttcgtgg tggtgctcct cgagccggcg gaagaccacg ccggcggcgt tgtccctgat
     7321 ccgcagggcg ggatagagcc tcgccacctt gggttcgagg ccatgctcga actcgatgcc
     7381 gacggtgagc cagagggaag cctggccgag ctcggccatc tgccgcggcg agggctcgta
     7441 ggagtggggg ctctggccgg gccccacgag ggcgaaggcg cggacgcggt ccccgccgac
     7501 gcgctcgacg aagtaggcct gggggaggac gctgaccgcg acgacgagct tcttggccgc
     7561 gggggccgag ccggcgacgc ccggaccggc cgcgaagcca aggccggctg cgaagaggat
     7621 ggaggacgcg gcgagcgcga gacggcggaa ctgcatgaag actctcctcg cccgcggaaa
     7681 gcgtgggagc gctcgccgtg gcgttttgcg aatgattcgc attcaatata cggggaacgc
     7741 caagacaatg caagcgacac gcgcgccggt cggggtttta atgaggccgc gcgacgatcc
     7801 gcggtcagtc ctcgaggacg agtatctcga cgcgccggtt cctggcccgg ttcgcctcgc
     7861 tgtcgttcgg gacgaggggc ttcgtcgcgc cgtagccgcg gtagaggagg cgccccgcgg
     7921 ggatgccgcg cgccgcgagc tcgtcgacca cgcgcttcgc gcgccggatc gagagggcca
     7981 tctcatccgc cgggcgcccg acggaggccg tgtgtccgcc cacgaggaag ctccggccgg
     8041 gaatgccctt gagggcgtcg gctatcgcgt cgagtctcca gcgctcctcg gggaggacgg
     8101 cgtcgccgtc ggcgacgaag cggaggtcct tcacggtgag gaggaggccc tcaggcgcct
     8161 tgacggcgct cacgcccgcc cccaggtcga gctcgggccc gccctcgacg aggaaggctc
     8221 ccggccccgc tccgctcgag gccgggcccg agaccgtgcc ggcgcccggg ccgccctcgc
     8281 cgccggccgg agccgcgcca ggcttgacgt ccgaggcggg gcctgccgcg gcagcggcgc
     8341 cgtcggaggc gccgtccgac cccgcggtgc cagaggccga gccggggccc gccgcggccg
     8401 cggcgccgcc cgaaggcgcc accccggcgc cagcggaagc cgcggtcgcc ggacctggcc
     8461 cgccgcctga gagggcgcgc ctgaggctcg ccgtcgcggc gccgtagtcg agagccaggg
     8521 tcgcgtcgta gaagacgagg aggaagccct tgagcctgac gctccggccc gaggcgtaga
     8581 cgaaggtctc gtcgaggctg tcgcggatga ggaggggggc gccggtctcc gcgtcgagga
     8641 ggatggtcgc gtcgtgggtc ccggtcgcgg agcggagctc gggatcgccc tgcctgtcct
     8701 ggccgcttct gtagcgcgtc gcgaacttcg cgctgatgga atgcgcctcc cggcccttgt
     8761 aggaggacag gccgcggtag acgtactcgg cgaggatggg gatgcgcgtc ggcctgcccg
     8821 agttctcggg atcggccacc ctgacgccct cggcctgcca gctctccccg ggggctacgg
     8881 ggccggcggg gaaagcggga aagccgcgga ggctcggata gccctcgtcg cggaggagct
     8941 cgaggccgcc gtcggaggcg acgaggaagt cggctgggat ctcctcctcc acgcggcgcg
     9001 agaccagggc cgcgtcgcgg agggtctcct cgaagacgag gaagccgccg cggtagcgga
     9061 cgccgccggc ctcgggctcg ccgtccagga gggccctggt ctcgcggtag acgtggccca
     9121 ggtaggcccc gtcgacgtac ttcgagaagt ccgagcgctc gacgagcttg tagcggcccg
     9181 tcgcggcgta gcggaaggag gcctcctggg cgccgagtcc cgggcgggga agggagaggc
     9241 agaggaggga ggcgaggagg aagatcgtca ctctcgccct cggccccggc atcagtgccc
     9301 ctcgcccctc gccatcttgc gcccgtgctc catgagggga gcgagggcgg cggcgcagaa
     9361 gcgcgcggcc ttctccgagc cggggaagtt cacgacgtac tggaggccgc gcatgccggc
     9421 ctcgccgcgg gagaagaccg cgttcggcgt ctcctccagg gagcgggagc ggaggtagtc
     9481 ggcgatgccc ggcatggagc ggtcgcagta ggccctggtc gcctcgggcg tgcggtcgcg
     9541 gggcgcgggc cccgtccctc ccgtcgtgag gatccagtcc gcctcggggt ggcgggagag
     9601 ggcggcgagg acggactcct cgccgtcggg cacgatctcc gcctcgatct ccgcgccggg
     9661 cagggcggac ttgagggtct cgacgacggc gggccccgag cggtccgcgt agacgccggc
     9721 ggaggcccta tccgatacgg taatgaccgc gatccgcaac ccgcacctcc ccgcccttca
     9781 ggacccgcat gaagatgccc ttcctcggca tcacgcagtc gcccacgagc ctgcggatct
     9841 cgcaggcggc gtggcactcc ttcccgatct tcgatacctc gaggacagcc tcgcccatct
     9901 cgattctcgt ccctatcggg agctcgtgca aggccaggcc gcgggtcgtg acgttctccg
     9961 cgaagtcgcc cggcttgagg ccgtccaggc ccgccttcgc gcaagagggg ccgcccgcct
    10021 tgaggacggc gctcgcggcc tcgatctcct ccgccgcgag gagggagacc tgccggtcct
    10081 ccgcgaggcc cgcgtgcgcg tcgcccgcga cgcccttccc ctcggcgagc gtgaaggagg
    10141 cgacggggcg cttgcgcgtg cccgtcctct ctgaaacgtt gagcgatacg atttcgaaac
    10201 cgccttctct cggcagcgcg cccatcagga tgcctccttc gtcttctcga ggaggcggat
    10261 cccgccgatc ctcatgttct tgtcgacggc cttgcacatg tcgtagaccg tcagggccgc
    10321 gacggagacc gccgtcaggg cctccatctc gatgcccgtc ttgtccgtgc agacggactt
    10381 ggcctcgatc gagatcccgc cgtcgtcgat gtccagcctt acctcgacgt gctcgatctc
    10441 cacgttgtgg cagagcggga tgagctcgct cgtccgcttc gcggccgtga tgcccgcgac
    10501 gcgggccacc gcgaggacgt cgcccttggc cagtgcgttc gccctcacca gggccaccgt
    10561 gcccggcgcc aggtcgatcc tgcctgcggc cctggccgtg cgcctcaccc tgccctttcc
    10621 cgagacgtcg accatgctgg ccttgccggc cccgtcgacg tgcgtaagct cgcttcccat
    10681 cctcatcccc ctatctggcc gacttccagg cccacgcaca cgtgcccgcg ctcgggcttc
    10741 gcgaagaccg ccctccggag gctcccctcg atgtccgaga agtccaccgg caccgagacg
    10801 ttcccgtgca ggcagggccg cagggtcccg tcggcgagga gcctgaggcg gtcgcagctc
    10861 ccgcacttgg gcggcctgtc gtagtcgccc ccgtcgcgct tctccgcgtc caggcggtag
    10921 cgcgcgatga actggatctc cgcgcccgcc gaggccgcga aggccctgag ctccccgaag
    10981 tcctcctccg tcgagtcctc cagggccacg acgttgagct tcaccggaag gcccgcggtc
    11041 ctggccgcct cgacgccggc cagggcctcg gagagctcgc cgccccgcgt caaggcccgg
    11101 tagcggaccg ggtcgagggt gtccagggag acgttgaccg acgagaggcc gcgcgccttg
    11161 agctcccccg ccagcggggc gagcagggtg ccgttcgtcg tcatcgcgag cttccggacg
    11221 cccgggaggg gcgcgagcat ggagacgagc ctctcgatgc ccttgcgggc gaggggctct
    11281 ccccccgtga ggcggatctt gccgaagccg aggcccgcgg ccgcggcggc gaccagggtt
    11341 atgcgctcga aggagaggat gtcctcgtgg cggaggagcc tcaccccctc ctcgggcatg
    11401 cagtagacgc agcggaggtt gcagcggtcc gtgaccgaga tccgcaggta gcctatctca
    11461 cggccgtagg agtcgagcat cgacccacgc tccttcctcg atccggtcca cgcctatctc
    11521 catcctgagg agagcgtcgg cctcggcgag cacgttgagc atcgacgagc cgtggtagcc
    11581 caggggcctc gcgagctcgc aagctccctc gcgcgcggac tcgcggccgg acgcggactc
    11641 ccgccctccg gcgccggccg cgtcggccgc gcccgagccc aggggagcgc gctccaggcg
    11701 ggcgggcagg aactccacgc ggtcggagcc gcggcgcgag aggggcgcgg ccaggcgggc
    11761 gcgtacgacg cgcggacggt actccaggcc catgcgccgc tccagatggg gccggaccag
    11821 gacctcgaaa ttcacgaagg tggagacggg attgccgggg aggccgaagg cggccttggc
    11881 gccgagggtc ccgaagtagg tgggctttcc ggggcgcatc gccaggttgt ggaagacccg
    11941 cctgacgccc agggcctcga ggctccgcgg cacgtagtcg aagtcgccca tggagacgcc
    12001 gccggagacg aggacgacgt cgcactcggc cagggcccgc ccgaggacgg ccgcgagggc
    12061 gcccggctcg tcggggaagg cgccgtagaa gcgcgcctcg cagcccgccg cccgggcctg
    12121 ggccgcgagc tgggggccgt tggagtcgta gatggaggcg gggcccaggg gcgagcccgg
    12181 cgcggcgagc tcgtccccgg tggagacgac gccgacgagg gggcggcggg cgacctggag
    12241 ctccgcgacg ccgcccgagg cgaggatgcc caggtcctgg ggcgcgagga ggcggggccc
    12301 gaggagctcg ccgccggcct tgaggttctc gccgcggcgg atgacgttgg agacggactc
    12361 gggcctcgtg aagcggacga agtcggcccc ggcggcgtcc ttccccgcgg actccgtcca
    12421 ctcgatccgc tggaccgagt cggccccgga gggaaggggc gcgcccgtca tgatgcgcgc
    12481 gcactcgccc ggggccaggg cgcgcgtcgg cgcggcgccc gcggcgacga gttcgacgac
    12541 gtggaaggcg ccgcccgccg cggccgcccc ggctcccgcc gccgggcctt cgccgcccgc
    12601 gccgaccgag gaggccacgg gcgcgccgcc gcagcaggcg cagccggccg cggccgcccc
    12661 ggagccggcc cgcgcgtagg cgaagccgtc catcgcggac ttgtcgaagg gcggatggtc
    12721 gatcgcggag aggaccgcgc gcggcagggc gcggcccagg gcctcggaca gcgcgacggt
    12781 ctcgagctcg ggcggcggcg ccgcctcgag gacgaggcgc agcgcctcgt cggggtggat
    12841 gggctcctgc ggcatcgccg cctccttatg aaagcgccgc gcggccgtcc tcgaggagga
    12901 gggtcctcgt ggccagggcc tcggcgagct tggggtcgtg ggtcgagaag actatcgtgg
    12961 cgccggcggc gcggcgggcg cgcagggcct cgaggacgag gcgggcgctc gcggcgtccg
    13021 cgctcgccgt cggctcgtcc aggaggagga cgtcggcccc ggccgcgaag gcgcgggcca
    13081 gggcgacgcg ctgggcctcg ccgccggaaa gctcgcggtg gccgcgccgc tcgaagcccg
    13141 agaggccgag gagggagagg gcctcgcgga ccccgtcgtc cgccgccccg cgggggaggc
    13201 cccgggcgcg ggccccgtag tagaggttgt acgacaccga gcctgagagg atgtagggcg
    13261 cctggtggag gtagacgccg cgcctccgca gggagcggga ggaggaggcc tcctccccca
    13321 ggaaaagaag gctgcccgcg gcgggcttga ggaggccgcc cagggccttg aggagggtcg
    13381 tcttccccga gccgttgggg ccgaggaggg cgaggcactc gccctccgcc acctcgaggc
    13441 gctgggccgc gaaggcggcc cagcggcgaa ggccgactgc gcctgcgcca ggctcgcgcg
    13501 ggccgcgctg ccggaggtgg cgggcgaggc cctgggagcg gcgcggctcg gggccgtagc
    13561 ggagctcgag gtcccgcgcc aggaagaggg gggcgccgct cacgaggctt cccggggcga
    13621 gagggcgtgg aggcagaggt tcacgcccag ggctatggcg aggaggacga ggccgaggga
    13681 gacggcccgt tcgaactcgc ccttcgccgc gtccagggcg atggccgtcg tcatcgtcct
    13741 cgtggagaag cggatgttgc cgccgagcat catggagacc ccgacctcgc cggtgacgcg
    13801 gccgaaggcg gtgacgacgg cggcgacgag gacggccctc gcctcgaaga gggttgaggc
    13861 gaggcggcgc agggggccgg cgcccaggct cagaatggtc tcgcggaagc gggggtccag
    13921 cttggagagg cccgcctgcg tgagggaggc gacgacgggg aaggccagga aggcctggcc
    13981 caggacgacg ccgcccgggg agtacatgag gtccaggaag cccagggggc ccgacctcga
    14041 gatgagggag tagacgagga ggccgatgac gaccgtcggg agggccatga gggcgttgag
    14101 gagggcgacg aggctccgcc gcccccggaa ggccctggtc ccgagggcca cccccgcggg
    14161 gatgccggga accagggcga agagggtcga gaagaaggag aagcggaggc tccttccgac
    14221 aatgccccag gtttcggcgt cgcccgagac gaggaggaga aaggcttccc tcaatcccgt
    14281 cccctactta tagaggtaga agcactgctc gcccgagatc ttgtaggcgg agatgagctc
    14341 cctgcccttc ggccccgtca tgaactccat gagggccttg gcgccggcgt agttggcggt
    14401 cggccacttg gcgggattga cggtgatgac gccgtagggg ttgaagagga cggggtcgcc
    14461 ttgcgagagg atcctgagct tggacttgtc cttcatcgcg agccaggtgg cgcggtcggc
    14521 gatcgtgtag ccctgcatct ggtcggccat cacgatgacc tgggacatgc cctggccggc
    14581 ctccttgtac caggcggcgc cgacgggact gacgccggcg gccttccaga cctcgagctc
    14641 cttcacgtgg gtgccggact tgtcgccgcg ggagacgaag gaggccccgg acttggcgat
    14701 cagggaaagg gcctccgtcg cggacttggc cttggcgagg ccggcggggt cggaggcggg
    14761 cccgaggacg atgaagtcgt tgtacatgac gtccttgcgg tcggcgccga agccggcggc
    14821 catgaaggcg tcctccaggg cgcgggcgtg gaccaggacg acgtcgcagt cgcccttctc
    14881 gccgatcttg agggaggcgc cggttcccac cgcgatgacc tcgaccttgt agccggactc
    14941 cttctcgaag acggggagca tgtaggcgag gaggccggac tgctcggtgc tcgtggtcgt
    15001 ggcgagcttg aggcggggat tcgcggggac cgcggcgggg gcctgggcga aggcgagccc
    15061 ggacagggct agcgcgagga cgagggcaga gaggcgcttc atggggtgct cctttccgaa
    15121 tgcgggaggc cggaggcggt ttcccgcccg gccctcggcc ggcctccctt cgggaatggt
    15181 ccggcctcga tcgccgcgcc ttgtgatgag gcttaaggga gcctttccga gggcgcaggc
    15241 gattcggagt ggctgtagag taacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnggctta agggagcctt tccgagggcg caggcgattc ggagtggctg tagagtaacc
    15421 atggatagta aatctgtcaa tctctctaat tccagcgatt gtcgggaggg cggccgcagg
    15481 ccgatgccgg cctttccagt ccctgaccgc ggaggagcat ggcggcgggg caggccttgc
    15541 gaggctttcc ctagcggctg gcggcgagcc tcccggcctc ctcgcagagc ccgcggttgg
    15601 ccgcggtcca ttcgggcggc agcgagaggc cgtgggcgta gggccgcccc tcggcgtcga
    15661 cggtgacgaa ggtcgcgaag ccccggatcg tcggctcagc ctcgtcgttg acaaagacct
    15721 cggcgccgat ggtgaggctc ttcgatccgc agcgcgccac ccgggccacc agctcgatcg
    15781 cgtcgccggg ccgcgcgggc ttcgtgaagc ggagcccgtg gatcatcgcg cagaccaggt
    15841 cctcaggccg gccgacgaag cgggccgcgg cgaggaagca gctctcggtg aaccactcgg
    15901 ccatgcggcc cgcgaacagc gtgccgtggt ggttgaggtc ctggctcttc acgagtctcg
    15961 tcgtccgggt ttccttgagc atgactcctc cttcgagcgg ggcggcgcgg ggcggcgcat
    16021 ggcggcgcat ggcggcgcat ggcggcgagg atccgctccg cggcccgccg ccaggagcat
    16081 gcgcgcggga ggcccgaggg tcaagaagga caggcccggc gttcgcctga ggcggcgaaa
    16141 atcagtatgt tcgacccatg gtgaagaacc tggcgatggc gatggccgcg gcgatcgcgc
    16201 tcctggcctg cgcggccggc gagcccggaa gcggaggcgc cgcggccccg cggggaaagg
    16261 agaccgatgc catgcaggct gaaggcaagc tcgaatacgc gatactcggt ggcggctgct
    16321 tctggtgcct cgaggcggcc tacgagctcc tccccggggt cgtctccgtg gagaacggct
    16381 acgcgggcgg ctcccgcagg aacccgagct acgagcaggt ctcctccggc gcgacgggcc
    16441 acgcggaggt cgtgcggatc gtctacgacc ccgccaaggt gagctacgag gagctcctcg
    16501 accttttctg gaagatccac gaccccacga cggaagatcg ccagggcgcc gacatcggca
    16561 gccagtaccg ctcggtgatc ttctacgcgg gcgaggagca gcgactggcc gcggaagcgt
    16621 cgatgcggga gacccagcgg cgcctcgtcg accccatcgt cacggagctc cttcccgtcc
    16681 ccgatttctg gatagccgag gactaccacc aggactactt caggcggaac ccggaggcgg
    16741 cctactgccg catcgtcatc gcccccaagc tcaagaaggc cggcttctag gcttccttgc
    16801 ctcaaggccc cgcgccgagc ccgcctcatg gccggcgcgg gcctgtcttc cggccgcccc
    16861 ggctcccccc cctccggccc ccctcgccgc ccttgcccgc ctccctcttg tcattcccga
    16921 gcgattcgct gtacgttaac actgttcaca tcttgaggag cggaggaaat ggcggtgggg
    16981 gtcgagggcg acaagggcac ggcggagcgc atactcgagg cggcggggga actcctcgcc
    17041 tccggcggct acgaagccct ctccatgcgc aagatcgccg cgatggtggg cctctcccag
    17101 gccgccatct accgccactt caaggacaag gacgagctcg tcggccgcct cgtcgcctcg
    17161 ggctacgccg agctcgtcgc gctggtggag tccctcgagg cggacggcgg cccgcccgac
    17221 caggtcctcg agcgcaacat cgaggcctac gtgggctggg ccctcggccg gccgaccgtc
    17281 ttcaaggccc tcctcctccg cgagatcggt ccggcggccg aggccgtgaa ctccctctcc
    17341 gaggggatag cccgcaggcg caggaccttc gccctcctcg ccgcccgcct caggcggggc
    17401 atggacgagg gcgtcttcga gccggccgat cccgagctca cggcccaggc ggtctggacc
    17461 gccatgttcg gcctcgcctc gcgcctggtc ttggagaagg accttccgcc cgaccgggcc
    17521 gccctcctca cggcccgcct ttcccgcatc gtcatccaag gcctccgccc tttgggggga
    17581 gagcccaaca acaaggaggg agcatgaagg ctctcgtcat ctacgacacg aagcacggat
    17641 cgagcgcgga agccgcgtcg gccgtcgccc gcggcatcgg ccccggcgcc gaggcgagga
    17701 ggctctccga ggccgccggc agcctcgagc cctacgacct cgtcgtcgtc gggggacccg
    17761 tctatttcgg caagtggtcg gagtcggccg gcgagtggct cgccgcccgc gcccccgagc
    17821 tcgaagggaa gcggctcgcc gtcttcgccc tgggctccat gcccgagctc agcgccgagc
    17881 ggatccgcgc gaccctgcct ccggccctca gggccaggct cgcggccgcg gtcgacttcg
    17941 gcggtcgtat cccggcggaa gggctctcct tcatcgagcg gaccctgacc cgcctcatcg
    18001 cgaaggccaa cgcgaagtcc ggagcggcga cccgcgagct cgacctcgcg gaggccgagg
    18061 ccttcggcgc ctcgctcgcg gccggaaagt gagggaggcg gacatgactg acatgaacaa
    18121 gcgcggacag cgcgtcgaag gccgcgaggc cgcccaggcg cgggccgcca aggccccggc
    18181 gctccgggcc ccggtactcc gggcggcctt cgcggccctc gccctggccc tcctctcctc
    18241 ctgcgcgacg ccgagcatcc ccctggccgt caccccgccg ctttcggccc tgaaggacgg
    18301 ccgctacgag gggagctacg acgccaagat ggtcaaggcg accgtcgccg tcctcgtcaa
    18361 ggacgggaaa atcgcggaag tgacgatcct ctcccacgac tcgagcccca tcgggaagaa
    18421 gggcgaggcc gtcgcggccc gcgtcgtcgc cgcgcagagc ctcgaggtcg aggccgtggc
    18481 gggcgcgacg ggctccagca gggccatcct caaggccatc gagaacgccc tcgcgggcgc
    18541 gggcccggac gcggcctcgg gggccaccga atgagccgcg cctcccgcgt cgtcctcgcc
    18601 gtcctttgcg ccctggtcct cctcctcgcc ctgggcttcg tggcggtctt cttctccttc
    18661 ggccccgggc gcgactcggg cgtccgtcca ttgggccctg cggccctggc cgacgggagc
    18721 taccgcggct cgggctcggc cggcgccccc ttcggcgcga tagccgcctt ccgcaaggtc
    18781 gaggtcgagc tcgaggtgcg ggacggccgg atcgagtccc tgcgctacct caagcccgag
    18841 accgaggggc ccgccgcgga ggtcttcgcc gagctctcga ggcgcgtcct tgagcgccag
    18901 ggacccgaca tcgacgccgt ctcgggcggc acctggactt cgcgggccct ccgcaaggcc
    18961 gtggagagcg ccctggagcg gggcggcgcg aagccccgct agccgatggc cctcgtaggc
    19021 ggggcgctcc gcgaggcgcc cttggccgcg cggcgccgcc ccggcggcgc aaggccccga
    19081 taggcgccgc gaggcgcggc cgagacgagg cgcggaaaaa cgggaagggc ggccgatcgg
    19141 ccgcccttct tcgttccgca aggccctcgc ctagcccctg acgccggcca ggaagccgtg
    19201 gaagcggtgg aggcccttgc cgtaggccat gaggggacag cccgcggcga cctccatgcc
    19261 cagggccgcg gcccgctcca gcgccgcctg ccccgcgagg ccgcggctcc ggaacaggac
    19321 ctttttgtag ccgagcttgc cgagctcctc caccgcctcc agggcccgcc cgcccgcgag
    19381 gaggacgaag gcctgcctcg tctccggggg gagctcgccg aggctggagt ggacgggcgc
    19441 ctcgagcttc gcggaggggc gcttgttcac ggcgtggacc gcgaagccgc cgccctccag
    19501 ggcctccttg atctccgcgg agtaggcggg gctcttcgac gaatagccga ggaaggccaa
    19561 ggggcctggc tcgaagaaat ccttggaaaa cttgcggatc atcatgggac gctccagggt
    19621 cttggggccc gctctcaggc tggcccggcc gcctacaagc ctagcgcttc ccgtcaggcg
    19681 gcgccagcgg ttccccggcg ccgacctccg ccgacgcgga gcctgcctgc gcggcgctcc
    19741 cctgcgcgcc ggcgtcgtcg ccgggttgcg cggcctgcgt ggcgctcccc tgcgcgcccg
    19801 cgtcgtcgcc gggctgcgcg gcctgcgcgg cgccgcccga ggcctgggcg gccgaggggc
    19861 ggttgagcca gagcccgccc acgatggccg cgagtcccag ggcgaagcgg aggttgaggc
    19921 gctcgccgcc gaagaggagg ccgaggagga ccgtgagggc cgtctcccag tagaagacga
    19981 cggagcctcg gctcgcgggc accgtccggt agccgtagga gaggatggcc tgggcggcga
    20041 agcctccgaa gcccaccgcg aagaggagga ggaggccggc cgctccgctg tggatgaggg
    20101 ggccggggcc aggcgcgaag gcgaggaggg ggaggccgaa gaggcagggc gagaggtaga
    20161 gcacgaaggg attctcgccg gcggccgagg ctctgcgcac aaagttgacc gcgaagcccg
    20221 agaagaccgc gctggcgagg gccaggaggt cgcccgagag ggcggcgccg ctcccgtcgc
    20281 ggacgacgag gaaggcgccg ccggcgcaga cgagcgcgct cgcgaaggtc ctagccccca
    20341 ccttctcgcc gaagaggagg gcgccgaaga aggcgacgaa gaggggatag gtgttggcga
    20401 ggagagtcgc gcgccccggc ccggtcaggg agacggcggc gtagctcgtg atcatcgcga
    20461 gggagccgaa gaagccgcgg aggatgacgg cgccgatgcg cttgggctta gtcccgggga
    20521 agcccaggga aagggtgacg acgcagaaga ccgcgcccag gaggaagcgc gaggcggaga
    20581 cgaagagggg cgagtagtag cgggaggcga gcttcacaca gagcgagacg agggcgagga
    20641 tcgtcgccgc gaggtagagg gccgcctggc ctttgacgcg caacttcaag aatgcctcct
    20701 cttcggggcg gggaatccta gctccccagg ccccgggccg gcaagggggc ccggacgggc
    20761 ggcttggagc ggaccgcccc cccgcgggcg gcgcgcttgc ctccgagagc gacaagcgcg
    20821 agaattgcgg catgggcgac gtgactagaa gaatgctcaa gctcggggcc ttcgccgccc
    20881 tcttctcgct cttccacttc gcctacgact ggtggccctc ggccatcacc gcagtcttct
    20941 cgggcgtgga cgagtccgtc ttccagcaca tgaagaccgg ctacttcgcc tggctcgccg
    21001 cctcggccct cgagtccctc gccgcgcggc cgccccgggg cctgcgccta ggcttcgcgg
    21061 ccgtccgcct ctggggcgcc ttcctcgtcg tctacctctt cgtctccctc tggtacctcg
    21121 gtccggccct cgtcggcccc atgccgagcg acgcggccga gctcgccctc gccttctcct
    21181 ccgtcgcctt gggcggcctc gcggccctcg gcctcgaggc ccagctcggg aaggcccccc
    21241 tctcccgcgg cctcgagctc ggcgccatgg ccgccaccct cctcgccctc tatctctttg
    21301 cccgcttcac cgcgaccagg ccctggatcg atctcttcga gctcccgccg ccgcattgag
    21361 cgccgggcct cccgaagccc tccttcgccc atcccgccgc gcaggcgcgg ggacggcgcg
    21421 gggatgtcgc gcgcggcgcg gggaaggcgc ccgcggccgg ctttgccgca tcggccgcga
    21481 ggaggtagat ttatagaagg ccgcgccgcg ccggaccgag aaggcgccgt cccgctgccc
    21541 gccgccgacg acgccccgcg gcgatctctc gcgtccaagg agtccccatg agcgacaccg
    21601 tcatcgtcta cgtcacccgc gacggctact cccgcgccct tgcggaggag cttggccgcc
    21661 tggtcggcgc gcccgccgcc gagatcggcg acgaggtcaa gaggaggggc ttcttcggct
    21721 atatgcgcag cggcttccag gcggcggcgc ggaaggccac gcccatccgc gacccgggcg
    21781 tggagctttc gagggcgaag cgcgtcgtcc tcgtccagcc cgtctgggcc tccgccgtct
    21841 gtccgcccct caggacctgg ctcagggccc accggcccga gctcgcgggg aagcgcatag
    21901 gcctggtcgc gagcgaaaag ggaagcccgc ctgagaagct caagctcgcc tacgagaccg
    21961 agttcggcga cctcgccgcc ttcgcctgcc tccgcgagag cgaggccggc gcggcgaaga
    22021 gcgcggccct ggccgccttc gcgagggagc tcggggcctg agtccggagg cgggccgagg
    22081 aagaaagcct gactaccgac gaacaagggc tgcccggctt gggcggccct cctttttagt
    22141 gactcgaaac gaaaggttcc ttcccccaga ctagagatgg aaggccttgc gcatgtccac
    22201 cttcttcgtg acgcggtagt gcaggtagat gaggaaggcg gcgatcggga ccagtgcgaa
    22261 gtccacgggc acggaccagc gcatcgcaag gccgcggccg cggttcaggc cgaccgtgag
    22321 gtcgatcccc gtgcagacga aggcggcgcc caggaaggcg aagcccacga cgttgaggcc
    22381 ccgcttcttc gccagggccg aggcgagggc gaccgaggcg acggcggcca gcgcgatggc
    22441 cgctatgggg aggccgtagg aggggaacca ctcgatgctc ccgtcgatgg cgtccaggag
    22501 gaagaggagg aggaaggcgg ccggccccag cgccgcgagg acgagccagg ggcggcggcc
    22561 gaggaagaag ggcaccgcga ggcagagcca gccgaagacg aggcccgtcg ccgggtaggc
    22621 cgaccaggtg acccgccggt tcgcgaggag gtcgatgagg aggagcatcg cgagggcgat
    22681 gcccatggag acggccgcga tctcgaccgc gaacttgagg cgctccttcg ccgagagggg
    22741 cgggagctcg tcggggacga cgtgctcggg ccagggcttg aggccgggac gcgcctcccc
    22801 ctcgtcgagc cgctggatgg gcgcgcggca gagcgggcag gcggggaccc cctcgtccac
    22861 ctcgacgccg catctcgagc aatagggcat gggagcctcc ttccctttcc ggtcagtcgt
    22921 tcgactcgag cttcacgggg atccccatcc tgcgcaggcg ggtgaagaag agccgctcca
    22981 gctcgctctc gcgcgccagg cgcgcgaagg agaggtagac cctgtccccc agggacagca
    23041 tcgcgcagtt ggtcccggtg aggggggccg gcgcggagac gaaatcggcg cgcgtcacgc
    23101 gctcccgcat cccctcgggc agctccaagg cccccagatt cgtgaggcag gaggagtaga
    23161 ggagctcgcc gaaacgcttg tagagggccc ggccgcccag gatcttgagg aagaggggca
    23221 tcgcgcgcac gaaggcgttc cgggtgctcg tgacgttccg cgtaatctgc cttgccacct
    23281 cgcgcggcgt cccctggagg cgcatggagt ggtgcacctc ctggacgatc tcctcgaact
    23341 cgaagtgccc gagcctccgg tcgacgccgg gcagggcgaa gagggagaag ttccgcatcg
    23401 tcctggaggg gaagggcttc cgcaggttga tgggcaccat gagcttgagg agggcgggct
    23461 tgcgtcggcg cgaccgctcg ggcagggcct cccagaggtc ctggagggcc gccacctgga
    23521 ccgcggccag gaactcggtg aggctgacgc gccgctcgcg cgcgagggcg aggaggggcg
    23581 cgaggggcat ggaggcgccc acgacgtggt agacgccgcg gcgctccagg cgcgcgggga
    23641 tgtggaaggc gaccttctcc ttctccggcc agggcaggga ggggtcgcag tatcgcttga
    23701 aggcgtcctc gaactcctcg tcctccggga cgcggccggg cctgaagacc gtcgggtcct
    23761 ccgcgaccag ggcgccgcgg agccgcaggt actcggcgac gaggctcttg aggaagatcg
    23821 aggcgccggt gccgtccgtg aggatgtggc tgaactcgac ggcgacgcgc cggtcgtagg
    23881 cccgcacccg gaagaggtag cggccgcggt cgcggaggcg gaggttcatg caggggtact
    23941 tggagtcggc catgacgcgg ggcggccggg agatcgtctc gaagtagtac cagaaggcgc
    24001 ctgcgtgcag ctcgacgttg aagtagggga agcagggatg gacccgctcc agggccgcct
    24061 ccagggtcga gaggcggatc ggctcgtcca gggtcatgga gaggcggaag agggtggaga
    24121 tcgtgccgga ggcgaaggtg ggaaagagga tggcggcgtt gtcgagcttg aaccagtgcc
    24181 tccgcctgcg ctggtccgct ccgcgcgggg cctccgtctt ttccgtcgcc atgcctacaa
    24241 ctatggcccc gcggccgcgg aggtcaaggg gggccgcgtg ggcgggccac cgcgcgcgat
    24301 ggccgcgcgg gccggggcgc cgggcgtctc gggccgccgg ccggcgcctg gggccgggcc
    24361 gcgagccggg cgagcacgaa agccccgccc ggctcccgcg gctagagact caggcggagt
    24421 tccagataga ggtagtcgtt gtcgatggtc cgcgagttcg agaaggtccc ccactcgcgc
    24481 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntagaggtag tcgttgtcga
    24601 tggtccgcga gttcgagaag gtcccccact cgcgctcggg atccccttcc tcgcggaagg
    24661 atccgtagat ccccaagccg cccttgaggt tcacgccgtc ggcgacgttg taggcgacgg
    24721 aggccgtggc gaggaagtcc cgcgcctcga ggctcgcgag gaagagggcc tcgggctgga
    24781 gggtctcgcc gaggagcttc ccggcgagcc tgaggctccc gacctgctcg aaggcgtagg
    24841 cctggccgtt gtacttcgcg agttcggcgg cgagcatctg gtcgcccggg ctcgcgtaat
    24901 cgctcgcttc atgatggtcg aagacgatcc gtggcgccca ggcgaggctc gcggtgaggc
    24961 ggctgtcgaa gaaggacttc gaggcctgaa cgacgcccga gagcgaagag ttggcgaggc
    25021 caaggtcagt cccatcctcg tccccggtga gagagagggc ccactcggtc cttaagtcga
    25081 agccgccggg cagccaggcg gcgtcgaggc cgaactggtt cacgcggccg tggaagggcg
    25141 agagggtgat aagcggagca ggtccgccga cgaccgtcac ctcgtagtcg gggaagcggt
    25201 tcaggtagga gagccagctc gccgaggccg agacgctgcc caggtagaag agggcgcgga
    25261 ggcccccctc gaagttcacg atggcgagcc cgggcgcctc gtcgtcctcg atgacgatcg
    25321 tcgtcggccc ggccgtgatc gtctgcgcga tcgagctcat gtcgttcggc acggagaccg
    25381 gcaggaagag tccctgcacc gtgccgaggt tccccggcag gttgtagacc aggttggccg
    25441 cgagggcgcc cagcttctgc tcgtcgagcg tcgagacgaa gcgggtgccg acgtgccggg
    25501 cgttcacgtt gtccgtgggg ttgttcccat cggcgaggcc ccaggcgacg acctgcttcc
    25561 cgaggcgggc gtccagggcg cctgaggaga actcgagcca ggcctccttc aggtccaggg
    25621 cggccatgag ccggccgttc cccgtcgcgg cgagggaaag gggatcgggc cgggccaggg
    25681 accagtccac cgcgtacgcg aggcccgcga ggttcgtgta ggcctccagg gagagccgca
    25741 ggccgacggc gtcaaaggcg tagccgtcca gggcgaggcc gccccggagg ccggccgcgg
    25801 cgaagggctc ctcggcgttc ggccgcgcgt aggcggaggc ggcgagccag cccgaggccg
    25861 cgaggtccag ggcgggagcc tcggcgaggg ccggcgcggc cgcggcgagg acgagaagga
    25921 gggcggcgag ggccgccatc gaagaccgcg cgcgcatcag cggcccctct ccaggaagcg
    25981 gagcgtgaag tagccgtcgt cgatggaggg gagattgacg ctcttcacga agacccactc
    26041 ggtcttgccg cccgtctcca ggttgctcat ctccatgctc tcgaactgcc agtacttccc
    26101 gtccacgagg cgctggcccc gcacgacaag gcgcttcacg agcttgccgc ccgcgtcgta
    26161 gtactcggac tgccggctcg tcgcgttctc cgcgtcgatc cagcgcacga ccttgccgta
    26221 gccgtacttc ttgagggtcg cgggtccctt ggaggcgtac tcgaccttga ggtacctgcg
    26281 cccctcgatc gtgtcctcgc ccagggcccg caccgcgaag tcggcgcggt ccagggtccc
    26341 gatgtcggcg aagctgaagt cggagcccag gaaggcgccc gtcttcgagt cgccgccgga
    26401 ggtgccgatg ctccgcacct tgcggagggc ggggatgtag atgtactgct cgtcctcctt
    26461 cccgccggcc tcgtagtcga ccgtgaggaa gcgggtgtcg cggacgtcgg cgggcgccag
    26521 gaagacgagg agctgccggt cggtgccgtc ggcctcggtc ttcgtgtagg ccttgatctc
    26581 gcggacgcgg gactggcccg ccttgttcgt gatcaccatc ttcatctccc cttccgtggc
    26641 gggggggcgg ggctgggtct cgttccgcct gaggagggac tcgaggtcct gggccggcag
    26701 gacagcggcg agggcggcgg cgaaagcggc ggccatgagc gttcgtttca tgttgactcc
    26761 ttcttcgcgg ggcccctcgg gccccgcgcg tggttctcta gagcgagggg ccgtcggcgc
    26821 gctcgtcccc gcatcgcgag gccagggctt tcttgccgac gaacttgggc cggacgagga
    26881 cgaggagcgc ggggaggagg gtcacagcga agaccgagct cgtcatcatg atgagggcca
    26941 tgaggaggcc catgaagaca ttgccccgaa gctggctgaa gaggagggcc gcgaagccgc
    27001 ccgcgaccgc gagggcgttc acgaagatgc ccttccccgt cgtgcgcatc gtctcctcga
    27061 tggcggcctc cagctccatg ccgcagagcg aaagcttgag gcgctccagg aagtgcaggg
    27121 tgtagtcgat gccgatgccg atgccgattg aggcgatggt ggccgtggag atgtcgatgg
    27181 ggatgccgaa ggccgccatg acgccgaagt tgatgatcag ggaggcggtg agcggcacga
    27241 tggagaagag gccctccacg aagctgcggg agatgaggga gatgaagagg aagcagacga
    27301 cgagggaggc gaggacggag ctcacctgcg aggagacgag gaggcggagg atcgcgaggg
    27361 tcaggttcga gaggcccgtg atgtcggccc tcgtccccgc ggggaattcc gcggcgatgt
    27421 aggagcgggc cctggcctgg acctggccga tgagacccga gctcgcggtc ttgaggaaga
    27481 cgttcacgcg ggccgtcttc cgcgggttgt cgatgaagcc ctcgatgttc tcgggcttgc
    27541 ccgagttctc gtactggaag acgagctggc ccgcgtagtc cctgccgggg acgagctcca
    27601 cggcgcggcc cgtcgccagg tcgaccttgc gctcggcgcc gcccgccgcc acgaaggcga
    27661 agccgccctc gacgcggagg ccgtcggcgg ggccgtaggc cgcgaggagc tccctcgtct
    27721 cggagtcgcg ctcctcgagg acgccgccct tcagggcgta gacctcggtc gccacgggga
    27781 ccggcgtcac gtcgtccggg accttgtcga aggcggggtc gtcctcgttc agggccttgt
    27841 tgatcccctt cacgaaggtg acgatcgact gcgagccgcc gacgagctcc ttcccctcgc
    27901 cgtagcgcgg atcctcgggc ccgaccactt tcttgccgac ggagcggagg gactcgagct
    27961 ccttctggag gccgtccatc gcgcggagga cggccgggtc caggatggcg ccctcctcgt
    28021 cggtcttgac gatgacggtc atcacggtga tgccggtgaa gttctcgttc aggtactcgg
    28081 aggcccgcct gatctcgctg ccggccttga aggagttcag ggtgttggtc tcgaaggaga
    28141 cgcgcggcgc gaaggcgccg aagccggcca ccagggccag ggaggccgcg agcacgagga
    28201 cgggcttctt cgcgacgaag cgccccgaag cggccaaggc ggaggccagc cgcgaatcgt
    28261 cgtgggcggc caccgtcctc gccttgggct tgggcatcag cctgaggagg gccggcgtga
    28321 gggtgaggct cagaaccagg gcaaagagga tgccgagggc cgagaggacg ccgaacatcc
    28381 tgagcgcggc gaggctggag aaggccaggg agccgaagcc gatcaccgtg gtcgcgccgg
    28441 caaggaagac gggcagggcg acgtgggtga ccgtcgactc caccgccttc tccttgtcct
    28501 ctatcgcgac caggtcctcg tagaagcggt tgatgacgtg gatcgtgtag gcggtgccca
    28561 ccgcgatgag gatgatcggg aggatcatcg tcgccatcgt gatgggctgg ccgatgaggc
    28621 ccatgaggcc catggcccag atgaccgaga ggacgacgtt gcccaggggc atgaagacgc
    28681 cgcgggccgt cctgaagctc aggaagagga tgagggcgat gacggcgatg acgacgggaa
    28741 agaggagagc caggtccttg atgaggagct tcccgacgat ggtggagacg gccttggcgc
    28801 ccgagacgta gaccttcccg tcgacgtagg agggcaggcc ctcgatggtg gccatgatcc
    28861 gctcgtagcc cgagaagggc gccgcggcgg ccttggcctt ccagcccttc gccgccgcga
    28921 gctccgcgac ggaagaggcg gccggctcca ggggcaggcc cggcgcgagg gccgcgagga
    28981 cggtcgtccg gcccttcttg tcagggccga ggctttcgcc gtagcgggcc ttggccgccg
    29041 cgcggtcggc ctcggagccg ggcagataga cccgcgcgac cgcgggagag ggcgcctcgc
    29101 cctccttcgc ggcggcggag acgccggaga gggcggcgag gaaggcctcg tcggccgagg
    29161 cgcccttggg cagcgttatg cgcacccgcg gcagggcctc cgcgtcgacg ccctccttca
    29221 tcgtgacgag gatggcggtg gactttccgt ccttcccgac cagggacttc gtcatgtcgc
    29281 tcgcctcgat cttggctcgg aaggcggcga tctcctcggc agtctccggc aggacgggca
    29341 tggcctcctc gatgacgagg ccctcctccg tgcccttgat gaggccggcc gagctgatcg
    29401 aaatgacgcc cttgacgccc tccacggcct ccagggcgcc gctccactcg cggacctgcc
    29461 tgagcatgcc cggcgagaag gcgccggagg agtcggtcag ggcgacgatg aggaagtcgt
    29521 ccgaggggaa gatcttcccg acctcgtcgt agaaggcctt ctccggcacg gtgtccggga
    29581 tgtcgtcggt catgtcggag tcgacccgca tgcccgcgag gccgaagccg aggcccaggg
    29641 tgaccgccag aaccagggcg atgacggtct tgggccgcga ggcgatgaag cgcgagaggc
    29701 ccttgaagct gattctcata gggatattcc tttgacgatg atctcgtcca ggctggcctt
    29761 caggctgtcg cagcctgcgc aggccagcgc gggcatgtcg agatggatga tgacgcccga
    29821 cagcatcgcg aagacgatgc cgccgagcac cttggggtcc tcgttcc
//
LOCUS       DAOOSJ010000006        25913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1320_length_25913_cov_6.149586, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000006 DAOOSJ010000000
VERSION     DAOOSJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 25913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25913
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_25913_cov_6.149586"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLG14_00750"
     CDS             <1..846
                     /locus_tag="PLG14_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47684.1"
                     /translation="AKPTLGGLVGGLLGWGFGLVLLCSFLANSGYFERFVVSYAGSLD
                     SGAAAAGKAAPRPAPRPAPPPLPALEEATIAGQVLRTIRAGGRERYEVPALGLSTHNK
                     AEAVSAIRLPRFVEATGLTDPGLLGRYLEADLNGSGRLSFDELEVFQRKTYRDFRYES
                     NLLALRPDEFLAAGGGDCEDFALYTAGLLRFWGWEPYLGSLGPASGGVGHAVCLSYEE
                     GSFPGGFTYFEVDGWTAEDGSSLKAGRYVPIDYDHVGSLSDAVRSGWKLRSIYLPEEA
                     WGLEM"
     gene            1089..1613
                     /locus_tag="PLG14_00755"
     CDS             1089..1613
                     /locus_tag="PLG14_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47685.1"
                     /translation="MNPRLKKLLPFLALLVGLLPVLGCATIMKGSDQKIAFQSDPTGA
                     SLSVFDEDGMLVAEGTTPITIPLKKGGGYFQAAKYRVVFEAPGRATKEVWLSGDLEGG
                     WYIAGNLLVGGFLGWLVVDPLTGAMWSLRPSNLTARLDKSVSATEDGGLRVVLADQLP
                     PELLAQATPLKPQS"
     gene            1727..2101
                     /locus_tag="PLG14_00760"
     CDS             1727..2101
                     /locus_tag="PLG14_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47686.1"
                     /translation="MEHKIRSRSLAAFVLIVAAVLSLGSCGSILMERDASVRFQNLTD
                     FDLYYGVKFGEAEYIGYVASGFSTGYLDTEAGTYSLQARDASGNWITISSGSMSVDAG
                     HSYTITGTGTGGSYYWILTQDS"
     assembly_gap    2310..2409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2410..3930)
                     /locus_tag="PLG14_00765"
     CDS             complement(<2410..3930)
                     /locus_tag="PLG14_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX47687.1"
                     /translation="MSGETPKTLLLVEDEAVVAMGEKAALEKRGYRVLLARSGEEAVE
                     AAKAEPGPDLVLMDIDLGKGLSGPEAAERILAGRHLPILYLSSHAEPEVVERAERVGS
                     YGFVAKSSPIAILDASIRMAFRLFEANEAVAQSEARLARAERVAKIGNWELRLAERRM
                     LASAGARAIYGVEEEELVLAEAQAFPLPEYRPALDRALADLVAGTAPYELDFKIRRRR
                     DGAVLDIHSLAEYDAGRRVVFGVIADVTEQARTREALREKSAELDLYFTSALDLLCIA
                     DTGGRFLRLNPEWEKVLGYGLAELEGRSFLELVHPEDLSATLAALAKLRDQEEILSFE
                     NRYRRKDGSYRWIEWRSRPLGETIYAVARDITDRKLAAQEVQALLAEKDLILKEVHHR
                     VKNSLNTASSLLSLQAEAVEDPEAKAALMDAQGRLQSMGTLYDRLYRSAEFQGISAKE
                     YLPALVDAIVANLPRRRAIEVESRIEDFSLDVKRAQALGIIVNELLTNVLKYAFEGR"
     gene            complement(3982..6615)
                     /locus_tag="PLG14_00770"
     CDS             complement(3982..6615)
                     /locus_tag="PLG14_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX47688.1"
                     /translation="MAAGRKTILLVEDEAIVAMAEKAALEKYGYAVVAVSSGEAAVEA
                     ARTSPGIELVLMDIDLGAGLDGTQAARAILAERELPILFLSSHTEREVVEKTERISSY
                     GYVVKDSSIAVLDASIKMAFKLFEANARLEASEARYRHSFDNAALGVCHVGLDGRFQE
                     VNLAFRRMTGYSEEELAGRAFADITLPEDRAVGADVFRRMVRGEMGSATFEKRYVRKD
                     GSVFWASVSTSLVRTGTLPDRFVTQVMDITGRVEAGERLKRSEAKYRNLYESIRDAVV
                     STDLAGNFTDANKVFLDLLGYSLEELRSLNFHELTPPEAREAERAAISRQLEEKGYSA
                     PYLKEYIRKDGSRVPAEISVFLVKDEAGVPTGLWGIVRDVTGRRQAEDALRKSKETAE
                     RLLGVAAEIIISEDLEGKVLLLNDSGHRILGYGPGELVGRNFFDLCVPEDARAEVRGY
                     FRRLGDEASDEILVHENDVLAKSGERKTILWRNAVIRDEAGKAVGLFSSGEDVTARRA
                     AERRLRERSEAYEAANEELRERERALLRQNEVFASLLRCIPIGVFMVEAPSGKPILAN
                     EAAAELLGRGVLPDVTKDSLAEVYASYKAGTSEPYPLAEMPVFLGMSGVTAHVDDMEV
                     RRPDGTKTLLEVFGTPVADEGGRVWASLVSFLDITEKKAAEARIRALLTEKELLLREV
                     HHRIKNNMATMGSLLSLQAGNTSDPFARAALADAEKRLRSMGLLYDRLYRSPSLVALS
                     AREYLEPLVDEILEKLMPGDKAVSVEKSIEDFTLDADRLQPLGIIVNELLSNALKHAF
                     KGRSRGRIAVSAWSRDGRFTLRVEDDGVGLPPSVDFDSAPGFGLRLVKGLAEQLGGTA
                     RIERGGGTRFVLEFGERGPAG"
     gene            complement(6771..7928)
                     /locus_tag="PLG14_00775"
     CDS             complement(6771..7928)
                     /locus_tag="PLG14_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOX47689.1"
                     /translation="MSRAASGRYVDISVTGDRAQAFIPDPLPPRLVPSADLRRLLDEA
                     LHALGGLSAAAPFFPDRDQLLYLYVRKEAVLSSQIEGTQSSLSDLLLFEHDAAPGAPL
                     DDVEEVSSYVAALNHGLERLRGGMPVSKRLLREIHGILLSTGRGSDKAPGEFRRSQNW
                     VGGSSPSRASHVPPPPDRVEGCMDDLERYLHSPEAKTEPLLAAAYAHVQFETIHPFLD
                     GNGRVGRLLIALVLTDAGLLSEPILYLSLYFKAHREEYYRLLDGVRSRADWESWLGFF
                     AVAARDTARQAVETARRVALLAQADRDRLAGLGRGMPKALAVFERFRVRLVRTIAQLS
                     GETGLAPNTVAKALADLEGLGIVRELTGKKRDRVYAYARLLEVMSEGTEPL"
     gene            complement(8051..8653)
                     /locus_tag="PLG14_00780"
     CDS             complement(8051..8653)
                     /locus_tag="PLG14_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX47690.1"
                     /translation="MIHFRYSSVECYLLPASGGRGLLAYDAGWPCSLHDYARAMKTTG
                     FRLGEIRWAMVSHFHLDHAGLVRDFQDAGIECLLFENQGGGIDEMERLIVRGHKDYRP
                     ILKDRFRRLRLGESRAFLAELGIRGEALATPGHSDDSVSLVLDDGDALVGDLPPASQV
                     MPDDEASLSSWRRIRELGGLQAWPSHAPPFRLDETLEGDG"
     gene            complement(8650..9522)
                     /locus_tag="PLG14_00785"
     CDS             complement(8650..9522)
                     /locus_tag="PLG14_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOX47691.1"
                     /translation="MSGASKIGSAKIGTCSWNYESWVGLIYSEPSRTAAGYLPEYSKR
                     FRTAEVDSWFYRLPERRDVLDYLRQVDEGFTFTVKVPEDISLTHLRNRGGGVLVPNPS
                     FLSPELFGRYLERIEPMLPRIDAVMLEFEYLNRDKMRSVDEFMKALDAFAAKAPKGVP
                     LAVETRNKNYLVEEYFRFLKERGLIHVFSEKIYLPHVYELYAKFGSLVEGTTVIRLLG
                     GDRAEIEKAAGGRWDRIVDEKPDKGRIAEMSLDLKYRGGKVVVNINNHYEGSAPITAR
                     FFEEAFGEASGAGG"
     gene            9679..10194
                     /locus_tag="PLG14_00790"
     CDS             9679..10194
                     /locus_tag="PLG14_00790"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOX47692.1"
                     /translation="MKEVILALANYNRGANLNLVETLEKAGTEVVTRETGTWYKTVLG
                     TIQHCFWYEALWLRRYKALGDYPALKAAILDEDLEARKAKVGDDFGKLVPLMKEVDSL
                     FVSFVEQASDEDLRKPIRFKNFKGEDQERIVWQTVFHVFNHFTHHRGEISGALDRMGV
                     GNDFAGFFRYL"
     gene            10207..10740
                     /locus_tag="PLG14_00795"
     CDS             10207..10740
                     /locus_tag="PLG14_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47693.1"
                     /translation="MLEPPTPAAMLSAFKEALSRQLKAALHTLGRAMETCPDAEWQQE
                     HGDAPFSQVVFHALFYADFYLGRDTHPFKAQPFHKAHAATFADYEELEDRKPVRLYEK
                     AFCREYLEHCLAKVDAAVGAETLESLAGESGIDFRRCGRMELYIYGERHIQHHAAQLG
                     LRVQQATGQELKWYSGH"
     assembly_gap    10973..11072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11484..11557)
                     /locus_tag="PLG14_00800"
     tRNA            complement(11484..11557)
                     /locus_tag="PLG14_00800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11521..11523),aa:Pro,seq:ggg)
     gene            complement(11626..12249)
                     /locus_tag="PLG14_00805"
     CDS             complement(11626..12249)
                     /locus_tag="PLG14_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX47694.1"
                     /translation="MDSESPSPNRRQLQARETKDRIYEAAWQLIGEEGYEEVSVERIC
                     ARAGIAKGSFYHHFRNKADLIVEGYSICDRYFEDEVSGRLKAAEGRERLVEFAVFQAR
                     YASELGVDLIRQVYKAQIENATRFFISPERGLPSVLRRIVAEEQAKGLLASDVSAEYV
                     TEFVLRFTRGLIYDWCLRNGDYDLPAAMEEASRRLLSVFSGPGKPSD"
     gene            12400..14670
                     /locus_tag="PLG14_00810"
     CDS             12400..14670
                     /locus_tag="PLG14_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47695.1"
                     /translation="MSDNQQRIRDLTGRFGTWYEDPAWLAKGRMTKELYPYDRMFSPI
                     RVNKLSIKNRLVMAPMGNISMAEETGRPNAKMIEYFAARARGGVGLITSGLVPVSHGI
                     DPSVTELGELSYFPRIDKSRTVFSGWRDVAERCRAYGARFFIQLTPGLGRVGSPECLV
                     NKLKLPVSASFNPNFYMPALPCRPLTDAQARRIVKNAGQAAADAKAATIDGVYLHGHE
                     GYLLEQMTNPAFNRRKLGAFRDWKAFGLELVREIRRRTGPDYPIMYRIDLSLALNETY
                     GKRMDSVRSLRKFKKERTVEETLEYMEALVREGVDLFDVDLGCYDNWWLPHPAGPMAP
                     GCFLEVARIAKERFAERGVRSNAGLPVPVVAVGKLGYPDLAEAALRDGSCDMVMLGRP
                     LLADPEWPNKAFAGEVEAICPCIGDQEGCINEFVEGGHPQCAVNPRTGFEELYDGREL
                     APAAKARKVAVVGAGPAGVTAALVAARRGHEVVLYDARKEIGGMLVPGSRPAIKYDIA
                     NYLSHLKAELAKAEASGRLRLELGKAPDAAALKAGGYDVILASTGSRQTRPPVPGIES
                     PNVVHAVDLLRDPALLGAAKSAVVVGGGVVGCETAYWLAHEKGVKTAVVEMLPEIMKG
                     VCTANRGWLIHYLEERGVPLLNCARLAEVRPGEAVVRRNVSKTVPDPLVTWTPLLPEN
                     IENPFAKKLKVEEVEERLPADLVVLAAGARPDDTLYRALVEARAAPEVRSLGDAFHLG
                     RVFEATKAAYAVATSL"
     assembly_gap    14679..14778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14828..16024)
                     /locus_tag="PLG14_00815"
     CDS             complement(14828..16024)
                     /locus_tag="PLG14_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47696.1"
                     /translation="MNSTRNVRLLIAVSFLQGFVFYGPVATLYRRAYGLALDELFLIE
                     SVSWILMLLLEAPWGWFADRFGYRRTLVAGNLLFAASKLVFAGASGFGGFLAERLVLS
                     VAVAAISGCDSALLYRSSREGEAERNFGLQSAAATAGIVLASLLSPLLYGVSLRWTAY
                     ATILPYSAAAALSFLLSDEGAPPPRERRFVLSSLKRLLSDRRLLLLLASGALAAEVSR
                     SLVVFLGQLQYERSGIPRAWFGLIFAALQLVPVVAGAAGALSRRFDRRAVILSCLATE
                     LLSVLVLLLAPARAAVSLLALAGVSASAALFAPYFQAVQNELVGTGERATALSLNAMV
                     VEGVAAAANLGVGRAAEASLSRGYLLCALLLAAALALSPLYLRLARPRAGKAAAGADG
                     RAGPRG"
     gene            complement(16073..16516)
                     /locus_tag="PLG14_00820"
     CDS             complement(16073..16516)
                     /locus_tag="PLG14_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX47697.1"
                     /translation="MKIDRAILMVDDEMIILLSLRQELRAKLGPAYRYEIARDADEGL
                     KAIEELAADGVSVVLVISDWLMPGMRGDEFLANVRAGHPEVKTIMVSGHADEDQLERL
                     RDSGALDAFFRKPWDPARLARECGRLLASPEAGVPPGAPPSVQPI"
     gene            complement(16513..18927)
                     /locus_tag="PLG14_00825"
     CDS             complement(16513..18927)
                     /locus_tag="PLG14_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX47698.1"
                     /translation="MTPVPAAPILGSATDRDFFELAPVALCLSDAGGRILAVNRRFAE
                     ELGYALEDLPTVESWFRAAYPDPAYRAETAAAWSAAVAGASGTGGFVEPGEFRVRRKD
                     GRDRVFQISASVSGGRILSSFIDVSDRVRARGEALQELLASGRLFLGDFGEAVRAVTE
                     ACSRYVGAGRVSVWRYSADYGEIECVDFYDRASGRHRSGERLPTAGFPEYVSRHRRGL
                     SVVAREVRGDPVASALPSGYLSAKDIRSLLDAPVWMGEAVRGILSFEAVGESRDWTAE
                     DERLAETMATVLSLGFEIAERKRAESGLAGREAALRAVLEAAPYPIVIQRGGPGVILA
                     ANRAYLESVGADRLEDILGKPVSEVSRAVDPRETAAMQAEIARSGKIDGMPYRSRRAD
                     GSVASSIVSVRRLEFEGGSAYVSAVVDVTELARARDELEALNAELERKVAARTDELVR
                     ANEGLAAANEGLARAMEELKAAQAQALASEKLAALGRISAGLAHELNTPLAAIAAAAR
                     TVRASLGPGFEELAASVSRLGPEGLALLGEARGLGDEESPEVDPQAERRAKRAARAEL
                     SAAGLADEEAEALGDELVELGLGRAVPRLSPFLAGPGGEALLAALRAVVGGRRAGRVA
                     EVSVEQAARVLSALRAYVRGGPGESPRPVGLAGAVGAMAELFKARARRGVRLRFDFAA
                     CEAVLGRPEELDRIWFNLLSNALHAVGDEGSVEVSSAIEGGLAVVSFADSGPGIPEGL
                     RGRIFEPFFSTKAPGEGTGLGLDIARRLARANGGDISFESRPGRTVFRVRLPVARNYG
                     PEASAR"
     gene            19035..20318
                     /locus_tag="PLG14_00830"
     CDS             19035..20318
                     /locus_tag="PLG14_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47699.1"
                     /translation="MDGGAGGTSFGAKYFPALGKRNFRIFWLGQCVSLVGTWMQNVGQ
                     SWLVLELTGSPAKLGLVSALQFLPMMLFSLVAGPFVDRFPKRKTLVATQGALLVLALA
                     LATLTATGLVRYWMVLVLAFLLGMVNLVDVPTRQSFVIELAGRESLVNAVSLNSAAFN
                     LARIFGPAAAGLLMEAIGIAPCFYLNALSFVAVIAALLAIDAPPLVGESRTGSVREVL
                     ASVREGLSYVRGKREIALPLVLMALISTFVINYNIYVPTFARGPLGRGASGYGFLMTA
                     MGAGSLAAALLLATRSGAAAAKGKGTAPGAARLFAGAIGMGACLALCGLQRSYALTAA
                     LLALTGFCTIVFTASANAGVQLASDDAHRGRVMSVYSLVFGGVTPVGSLYAGSLTDAA
                     GPAAAMAASGAVGLAASLAIGLAARRRRDARRTGP"
     gene            20539..21567
                     /locus_tag="PLG14_00835"
     CDS             20539..21567
                     /locus_tag="PLG14_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47700.1"
                     /translation="MKHRMLVLAAVAALAAASLAFAAPPAQAKAVPVYPGAAADPAAA
                     DYAKELSNGEGDPDLVSEEVIAYATAAAPEEVFAWYRQKLAAKPMRDITQAEYESGST
                     PPMYYLEPYEEEDFRDGTTGDRKVVVKYEGAWIREQLKAKRKPLEGAYLSSGSFSWML
                     REKDGRARGIVVTVLDASFLPYIGEGPGAGPATLGKKNYAQKTYVFVYSQLRKSEGEA
                     GAEREERRDAEGEADMEAARAYFAANPPSAKELGLPLYPGAAFDAENSAGMSMDEGMK
                     AWLFTTADSLDKVVAFYEKASGRKAERLGQARRIVLKGSGLMPEHFLALEPGAAGGTA
                     ISFFKTEE"
     assembly_gap    21649..21748
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21749..22312)
                     /locus_tag="PLG14_00840"
     CDS             complement(<21749..22312)
                     /locus_tag="PLG14_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HOX47701.1"
                     /translation="MSRTSAGGRSPRPAGARASAREAGGAGDARAAGQVRGARRGAGP
                     EASAYDPAIAAFLEEVYEACAAGPRLRRDPLALVVPFDDPRDREVAGLFASTLAFGSV
                     DLILRAVRAALEPLGERPASALAAMGESGLREAWGGFQYRYCFPKDMIALARGARRAL
                     MEAGSLEDFFLRGDPGGGDVAAAAGSFA"
     gene            complement(22309..>22912)
                     /locus_tag="PLG14_00845"
     CDS             complement(22309..>22912)
                     /locus_tag="PLG14_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX47702.1"
                     /translation="GAAALSPLEESLRAAARAEAARLLLLDAAASYDWALAASYLPRA
                     KAAPGFDDRALSGLVRRRSGDWAGALEEAAAWYKERPESEAAAEAYARALLGTKAEKA
                     ASDLAARLLAGKGSPAFRSSLFWLQAQLAKSDDAALSALRSALVENPDNSEALAAMCD
                     IYLRRRDFQKARFYLKQALALSPGDPELKRRSRELEASAP"
     assembly_gap    22913..23012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23013..24247)
                     /locus_tag="PLG14_00850"
     CDS             complement(<23013..24247)
                     /locus_tag="PLG14_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX47703.1"
                     /translation="MRRGRGAERPAALVPLLLSILLSCLSCAGAPAAPAAPASSPQPG
                     SEPGAAGGGTALKGAAPQALSRALEERRAVEAAVVFGSPSSIAKAFSLIAEATSLKEE
                     EAFSLAWMGARIALLVYPESADSFVPADLRGQAELPASPLARSLADAAAGRVPALLPE
                     AEGTPLGELVPALAAFRSDSRETARRCLEALDRFERLGAPSVLPGLVRGLDAERRKDF
                     ARALDYYAGVRSAAPDAWPASLGAARAYLALGRPAAAEELLRQVDPAAADSPPIRRVR
                     ARALYDLGRLSEAEPLVLRILLEDPQDSAFVLVRAHLLARSRSFQQAQPLLDAYGTVD
                     PSNRRYLALRAEVAEGLRGRDEALKWARRGLAAYPDDPELLAVAARLLFAVPSTLRAD
                     LVEAARSEGRELARRAEGQA"
     gene            complement(24247..24801)
                     /locus_tag="PLG14_00855"
     CDS             complement(24247..24801)
                     /locus_tag="PLG14_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47704.1"
                     /translation="MSPSRSRNDDLLFIGALAAVIAGVGFLLRTTGIAPEAERFWPLM
                     VLAAGGLLLYLAIVRRFSDPFFFAGVLFSLSGLLMIAGVFAGWTFERAWPLLMAAAGL
                     SWLAAGLRRFRRLRPQGAVPALIFVFLGFFFCLFSFDLVRVGFRQFISEWWPTVLIAG
                     GAALFAAYGASGRKRGRKGRPGRP"
     gene            24872..>25913
                     /locus_tag="PLG14_00860"
     CDS             24872..>25913
                     /locus_tag="PLG14_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX47705.1"
                     /translation="MAKPRSGAPARPRSAERALLAAAFAAAALLGSACSSPRAAYLSG
                     GRQDRAELAELFGLLDSQSADQVERFAAVRQVSSILLRQKEYGRLTALLTSIAAERPQ
                     DPYAAWYLFAAAYAYDQRGSAPIAALYYDRIVKNYPDLEVDGKSIHYECLLRLIETEE
                     APERRIEYYKDLIARFPGRVDMGRILFLLGKEYERVGDWELAVKTYAKFLPYFGASIP
                     GYPDAFQYARNVIEFYNSPKDWAYEDLQTLVGNIKAALQAGDPYRLRRYRAKVNFFAM
                     SWHQDETDVNSQVLFDFSEFMSGGRVYSAEAMDASSGPREAYLKTWGWSERISTWYLY
                     FRKIYFPADPEIH"
BASE COUNT         3820 a         9085 c         8928 g         3580 t
ORIGIN      
        1 gcgaagccta ccctgggcgg gctcgtaggc gggctcctgg gctggggctt cggcctcgtc
       61 ctcctctgct ccttccttgc gaactccggg tacttcgagc gcttcgtcgt ctcctatgcg
      121 gggagcctgg actctggcgc ggccgcggcc ggcaaggcgg ccccccgccc ggcgccccgc
      181 cccgcgccgc cgcccctgcc cgccctggag gaggccacga tagccggcca ggtcctgcgc
      241 accatccgcg caggcgggcg cgagcgctac gaggtgccgg ccctgggcct ctcgacccac
      301 aacaaggcgg aggccgtctc cgccatcagg ctgccgcgct tcgtggaggc gacggggctc
      361 acggatcccg gcctcctcgg gcgctacctg gaggccgacc tgaacggctc gggccgcctc
      421 tccttcgacg agctcgaggt cttccagcgg aagacctacc gggacttccg ctacgagtcg
      481 aacctcctcg ccctccgccc cgacgagttc ctcgcggccg gcggcggcga ctgcgaggac
      541 ttcgccctct acacggcggg cctcctccgc ttctggggct gggagcccta cctcggctcc
      601 ctgggccccg cctcgggcgg cgtgggccac gcggtctgcc tctcctacga ggagggctcc
      661 ttcccgggcg gcttcaccta cttcgaggtc gacggctgga cggcggagga cgggagctcc
      721 ctcaaggcgg gacgctacgt ccccatcgac tacgaccatg tcgggagcct ctccgacgcc
      781 gtcaggtcgg gctggaagct ccgctccatc tacctcccgg aggaggcctg gggcctcgag
      841 atgtaacgga agttccgtaa ccggggaacg gaggttacgc gcttttagtg ccgcgcgccg
      901 cgagggcgcg gcgggacgag gcctctcgcc cgatgcggcc ggggcgggcc ggggcgggct
      961 ctggcgaggc gccggctcca acgggagccg tgccaggccg tcaaggcgcg ccccggaggg
     1021 agaccgcgcc ggacttggca cggtgcttgc tttacaggat gtacgaaacc atttccaaag
     1081 gagtcgccat gaaccctcgc ctcaagaagc tcctcccctt cctcgccctc ctcgtcggcc
     1141 tcctccccgt cctgggctgc gccacgatca tgaagggctc cgaccagaag atcgccttcc
     1201 agagcgaccc cacgggcgcc tcgctctcgg tcttcgacga ggacgggatg ctggtcgcgg
     1261 agggcacgac gcccatcacg atccccctca agaagggcgg cggctacttc caggccgcca
     1321 agtaccgcgt cgtcttcgag gcgcccggcc gcgccacgaa ggaggtctgg ctctccggag
     1381 acctggaggg cggctggtac atcgcgggca acctcctcgt gggcggcttc ctgggctggc
     1441 tcgtcgtcga tcccctcacg ggcgcgatgt ggagcctgcg gccctccaac ctcacggccc
     1501 ggctcgacaa gtccgtctcc gcgacggagg acggcggcct ccgcgtcgtc ctggccgacc
     1561 agctgccgcc cgagctcctg gcccaggcga cgcccctcaa gccgcagtcc tgaaggccgg
     1621 ccaggcccaa ggctgatgaa aaatgatgta agaataatca caaaaccatg taaaaaagtc
     1681 gccttttcgt ccgacaacca ggcgcgtcat gcgcgaagga gcaacgatgg aacacaagat
     1741 ccgctcgagg agcctcgccg ctttcgtact gatcgtcgcc gcggtcctct cgctcggctc
     1801 ctgcggctcg atcctcatgg agcgcgacgc cagcgtgcgc ttccagaatt tgacggactt
     1861 cgacctctac tacggcgtca agttcggcga ggccgaatac atcggctacg tcgcctcggg
     1921 cttctcgacc ggctatctcg acaccgaggc ggggacctac tcgctccagg ccagggacgc
     1981 gagcggcaac tggatcacga tcagctcggg ctcgatgagc gtcgacgccg gccatagcta
     2041 cacgatcacc ggcacgggca cgggaggctc ctattactgg atcctcaccc aggactcgtg
     2101 acggcgggcg cgggggcgcc ccctccccct tactagaacc ccgcgccgcc ccgccgcacg
     2161 agcttccgcc ccaggctcat cgcctccagc tccgcctcga cctggaggat gaaggagact
     2221 ccaggatcct cgaagaggtg gcgcaggggc tttccgtcga tctcgcgctc gatggtcacg
     2281 ccgactgcgt agcggccgtc ggggcgcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnng cggccctcga aggcgtactt gaggacgttg gtgaggagct cgttgacgat
     2461 gatgcccagg gcctgggcgc gcttcacgtc cagggagaag tcctcgatcc gcgactcgac
     2521 ctcgatcgcc cggcgccggg gcaggttggc gactatcgcg tccaccaggg cgggcaggta
     2581 ctccttcgcg gagatgccct ggaactcggc ggagcggtag aggcggtcgt agagggtgcc
     2641 catgctctgc aggcggccct gggcgtccat gagggcggcc ttggcctccg ggtcctcgac
     2701 cgcctccgcc tggagggaga ggagggagct cgccgtgttc aggctgttct tgacgcggtg
     2761 gtggacctcc ttgaggatga ggtccttctc cgcaaggagg gcctgcacct cctgggccgc
     2821 gagcttgcgg tccgtgatgt cgcgggccac ggcgtagatg gtctccccca gcgggcgcga
     2881 gcgccattcg atccagcggt aggagccgtc cttgcggcgg tagcggttct cgaaggagag
     2941 gatctcctcc tggtccctga gcttcgcgag cgcggcgagg gtggcggaaa ggtcctcggg
     3001 gtggacgagc tcgaggaagg agcggccctc gagctcggcg agcccgtagc ccaggacctt
     3061 ctcccactcg gggttgagcc tgaggaagcg gccgcccgtg tcggcgatgc acagaaggtc
     3121 cagggccgag gtgaagtaga ggtcgagctc cgcgctcttc tcccgcaggg cctccctggt
     3181 cctggcctgc tcggtgacgt ccgcgatgac gccgaagacg acgcgcctgc ctgcgtcgta
     3241 ctccgcgagg gagtggatgt ccaggacggc gccgtcgcgc ctgcgccgga tcttgaagtc
     3301 gagctcgtag ggggccgtgc ccgccacgag gtcggccagg gcccggtcca gggccggccg
     3361 gtattcgggg agggggaagg cttgggcctc ggccaggacg agctcctcct cctcgacgcc
     3421 gtagatggcc cgcgcgccgg cggaggcgag catccgccgc tccgccaggc ggagctccca
     3481 gttgccgatc ttcgccaccc gctccgcgcg ggccaggcgc gcctcgctct gggcgacggc
     3541 ctcgttcgcc tcgaagagcc tgaaggccat ccggatggag gcgtccagga tcgcgatggg
     3601 cgagctcttg gccacgaagc cgtaggagcc gacccgctcg gcccgctcca cgacctcggg
     3661 ctcggcgtgg ctggagaggt agaggatggg aaggtggcgg cccgcgagga tgcgctcggc
     3721 ggcctcgggg ccggagaggc ccttccccag gtcgatgtcc atgaggacga ggtcggggcc
     3781 cggctcggcc ttcgcggcct ccaccgcctc ctcgcccgag cgcgccagga ggacgcggta
     3841 gccgcgcttc tccagggccg ccttttcgcc catcgcgacg acggcttcgt cttccacgag
     3901 gaggagggtc ttgggggtct ctccgctcat ggtttgacta tcggcgccaa gtgcttcgcg
     3961 cgcaagccct ttcccgggcc cctagcccgc cgggccccgc tccccgaact cgaggacgaa
     4021 cctggtcccc ccgccgcgct cgatccgcgc cgtgccgccc agctgctcgg ccaggccctt
     4081 cacgagcctg agcccgaagc ccggcgcgct gtcgaagtcg accgaggggg gaaggccgac
     4141 gccgtcgtcc tccacccgca gggtgaagcg gccgtcgcgg ctccaggcgg agacggcgat
     4201 gcggccccgg ctcctgccct tgaaggcgtg cttcaaggcg ttggagagga gctcgttgac
     4261 gatgatcccg aggggctgga gccggtccgc gtccagggtg aagtcctcga tgctcttctc
     4321 gacactgacg gccttgtccc cgggcatcag cttctcgagg atctcgtcga cgaggggctc
     4381 gaggtactcc ctggccgaca gggcgacgag gctcggggag cggtagagcc ggtcgtagag
     4441 gaggcccatg ctccgcagcc gtttttccgc gtccgccagg gccgccctcg cgaaggggtc
     4501 cgaggtgttg cccgcctgca gggagaggag ggagcccatc gtggccatgt tgtttttgat
     4561 gcggtggtgg acctcccgga ggaggagctc cttctccgtg aggagggccc tgatcctcgc
     4621 ctccgcggcc ttcttctccg tgatgtccaa gaagctcacg aggctggccc agaccctccc
     4681 gccctcgtcg gcgacgggcg tgccgaagac ctcgaggagg gtcttggtcc cgtcgggcct
     4741 ccggacctcc atgtcgtcca cgtgggcggt gacgccgctc atgcccagga agacgggcat
     4801 ctccgcgagg gggtagggct ccgaggtgcc ggccttgtag gaggcgtaga cctccgccag
     4861 gctgtccttg gtgacgtcgg gaaggacgcc gcggcccagg agctccgcgg cggcctcgtt
     4921 cgccaggatg ggcttcccgg aaggggcctc caccatgaag acgccgatgg ggatgcagcg
     4981 caggagggag gcgaagacct cgttctggcg caggagggcg cgctcccgct cccgcagctc
     5041 ctcgttcgcc gcctcgtagg cctcgctcct ctcgcggagc cggcgctcgg ccgcgcggcg
     5101 ggcggtgacg tcctcgcccg agctgaagag gccgacggcc ttcccggcct cgtccctgat
     5161 gacggcgttc cgccacagga tggtcttccg ctccccggac ttcgcgagga catcgttctc
     5221 gtggaccagg atctcgtcgg aggcctcgtc ccccaggcgc cggaagtagc cgcggacctc
     5281 ggcccgcgcg tcctcaggca cgcagaggtc gaaaaaattc ctcccgacga gctcgcctgg
     5341 cccgtagccc aggatccggt ggccggagtc gttgagaagg aggaccttcc cctccaggtc
     5401 ctcggagatg atgatctccg cggccacgcc caggagccgc tcggccgtct ccttgctctt
     5461 gcgcagggcg tcctccgcct gcctgcgtcc cgtgacgtcg cggacgatgc cccacaggcc
     5521 ggtcgggacg cccgcctcgt ccttgacgag gaagacggag atctctgccg gcaccctgga
     5581 gccgtccttc cggatgtact ccttcaggta gggggcgctg tagcccttct cctcgagctg
     5641 gcgcgagatg gccgcgcgct cggcctcgcg ggcctccggc ggcgtgagct cgtggaagtt
     5701 cagcgagcgc agctcctcga gcgagtagcc gaggaggtcc aggaagacct tgttcgcgtc
     5761 cgtgaagttg cccgcaaggt ccgtggagac gacggcgtcc cggatcgact cgtagaggtt
     5821 ccggtacttc gcctcgctcc gcttgagccg ctcgcccgcc tccacgcgcc cggtgatgtc
     5881 catgacctgg gtgacgaagc ggtcgggaag ggtccccgtc ctcacgaggg aggtggagac
     5941 cgaggcccag aagacgctcc cgtccttgcg gacgtagcgc ttctcgaagg tggccgagcc
     6001 catctccccc cggaccatgc gcctgaagac gtccgcgccg acggcccggt cctccggcag
     6061 ggtgatgtcg gcgaaggcgc ggcccgcgag ctcctcctcc gaatagccgg tcatccgccg
     6121 gaaggccagg ttgacctcct ggaagcggcc gtccaggccc acgtggcaga cgcccagggc
     6181 ggcgttgtcg aaggaatggc ggtagcgggc ctcgctggcc tccagcctcg cgttggcctc
     6241 gaagagcttg aaggccatct tgatggaggc gtcgagcacg gcgatggagg agtccttgac
     6301 gacgtagccg taggagctga tccgctccgt cttctccacg acctcccgct cggtgtggga
     6361 ggagaggaag aggatgggca gctcccgctc cgcgaggatg gccctggccg cctgggtgcc
     6421 gtccaggccg gctcccaggt cgatgtccat gaggacgagc tcgatcccgg gactggtcct
     6481 ggcggcctcc accgcggcct cgccggagga gacggcgacg acggcgtagc cgtacttctc
     6541 cagggcggcc ttctccgcca tggcgacgat ggcttcgtct tccacgagga ggatggtctt
     6601 ccgccctgcc gccatgcccg ccttccgtcc ggccgccttc cgcggcccgc tgtccgtcag
     6661 tagtagtata ggcctgagac cggatttcag ccatcgagtt caggcgcggg gaaggccgcg
     6721 gccgcccccg cgcgccgcgc gccgccttcg gccacggctt gggttcgcgt ctacagcggc
     6781 tcggtgcctt ccgacatcac ctcaaggagc cgcgcgtagg cgtagacgcg gtctcgcttc
     6841 ttcccggtga gttcccggac gatgccgagg ccctcgaggt ccgccagggc cttcgcgacg
     6901 gtgttggggg caagcccggt ctccccgctg agctgcgcta tggtcctgac caggcggacg
     6961 cggaagcgct cgaacacggc aagggccttc ggcatgcccc ttccgagccc cgcgagccgg
     7021 tcgcggtcgg cctgcgcgag gagcgcgacg cgtcgcgcgg tctctacggc ctggcgggcc
     7081 gtgtcccgcg ccgcgacggc gaagaacccg agccaggatt cccagtccgc gcgggagcgc
     7141 acgccgtcca ggaggcggta atactcctcg cggtgcgcct tgaagtagag cgagaggtag
     7201 aggatcggct cggagaggag gcccgcgtcg gtcaggacga gggcgatgag cagccgcccc
     7261 accctgccgt ttccgtccag gaagggatgg atggtctcga actggacatg ggcataggcc
     7321 gccgcgagga gcggctccgt cttcgcctcc ggagaatgaa ggtagcgctc caggtcgtcc
     7381 atgcagccct cgacccggtc cggcggcggc ggaacgtggg atgcgcgcga gggcgaggag
     7441 cccccaaccc agttttggct ccgcctgaac tcgccgggcg ccttgtcgga gccgcgtccc
     7501 gtcgacagga ggatgccgtg gatctcgcgc aggaggcgct tggagacggg catgccgccc
     7561 ctgagccgct ccaggccatg gttgagcgcg gcgacgtagc ttgagacttc ctcgacgtcg
     7621 tccaggggcg cgcccggcgc ggcatcgtgc tcgaagagaa ggaggtcgga aaggctcgac
     7681 tgggtgccct cgatctgcga ggaaaggacc gcttctttcc gcacatagag gtagaggagc
     7741 tggtcccggt cggggaaaaa aggcgcggcg gcggaaagcc cgcccaaggc gtggagagct
     7801 tcgtcgagga ggcgccgcaa gtccgcggaa ggaaccagcc tgggcggcaa gggatcgggg
     7861 atgaaggcct gggccctgtc gcccgtcacc gagatgtcga cgtaacgccc gcttgccgct
     7921 cgagacacgc catgctcctc gcctgcgggg aatatcccgc ttgactattt caatatagta
     7981 gcatttttgc attagcgcca ctcgctattt caggaatcgc catatccgca aggatacggg
     8041 agcgccggtt tcagccatcg ccctcgagcg tctcgtcgag cctgaagggc ggcgcgtggc
     8101 tcggccaggc ctggaggccg ccaagctccc tgatccgccg ccagctcgaa aggctcgcct
     8161 cgtcgtcggg catgacctgg gaggccgggg gcaggtcccc gaccagggcg tcgccgtcgt
     8221 ccaggaccag ggagacgctg tcgtccgagt gacccggtgt cgccagagcc tcgccccgga
     8281 tcccgagctc cgcgaggaag gcgcgggact ccccgaggcg cagccgcctg aaccggtcct
     8341 tcaggatggg gcggtagtcc ttgtgcccgc gcacgatgag ccgctccatc tcgtcgatcc
     8401 cccctccttg gttctcgaag aggaggcact cgatcccggc gtcctgaaag tcccgcacga
     8461 gtcccgcgtg gtccaggtgg aaatgcgaga ccatcgccca ccggatctcc cccaggcgga
     8521 agccggtcgt cttcatggcc ctggcgtagt cgtggaggga gcagggccag cccgcgtcgt
     8581 aggcgaggag gccccggccg cccgacgcgg ggaggaggta gcactcgacg ctcgagtagc
     8641 ggaagtggat catccgcccg ctcccgaggc ttccccgaag gcctcttcga agaacctcgc
     8701 ggtgatcggc gccgagccct cgtagtggtt gttgatgttg acgacgacct tgccaccccg
     8761 gtacttgagg tccagggaca tctccgcgat ccgccccttg tcgggcttct cgtccacgat
     8821 cctgtcccag cgccccccgg cggccttctc gatctccgcg cggtcgccgc ccaggagcct
     8881 gatcaccgtc gtcccctcca cgagggagcc gaacttggcg tacagctcgt agacgtgggg
     8941 caggtagatc ttctcggaga agacgtggat gaggccccgc tccttgagga agcggaagta
     9001 ctcctccacg aggtagttct tgttgcgcgt ctccacggcg agggggacgc ccttgggcgc
     9061 cttcgcggcg aaggcgtcca gggccttcat gaactcgtcc accgacctca tcttgtcccg
     9121 gttcaggtac tcgaactcca gcatgacggc gtcgatccgc ggcagcatgg gctcgatccg
     9181 ctcgaggtag cggccgaaga gctcgggcga gaggaaggag gggttgggga cgaggacccc
     9241 gccaccccgg ttccgcaggt gcgtgaggga gatgtcctcc ggaaccttca cggtgaaggt
     9301 gaagccctcg tccacctgtc tcaggtagtc gaggacgtcg cggcgctcgg gcaggcggta
     9361 gaaccagctg tcgacctccg ccgtgcggaa gcgcttcgag tactcgggca ggtagcccgc
     9421 ggcggtcctg ctcggctccg agtagatgag gcccacccag gactcgtagt tccaggagca
     9481 ggtgccgatc ttcgccgagc cgatcttcga cgcgccgctc atcgccgccc cctctcgggg
     9541 ctccgcgccg ggcggtccgc gaaacgctcc ctctcaggcg acaccggccg ggccagggtt
     9601 ttctcgtcca tttccgcgct ccttcccctc cacccagagt attattgatc cagaggccct
     9661 tgagaaggag cggttgcgat gaaagaagtg atcctcgccc ttgcgaacta caaccgaggc
     9721 gcgaacttga acctggtcga gacgcttgag aaggccggga ccgaggtggt caccagggag
     9781 accgggacct ggtacaagac ggtcctcggg accatccagc actgcttctg gtacgaagcc
     9841 ctgtggctca ggcgctacaa ggcgctgggc gactatccgg ccctgaaggc cgccatcctc
     9901 gacgaggacc tggaggcccg gaaggcgaag gtcggcgacg acttcgggaa gctcgtcccc
     9961 ctcatgaagg aggtggactc gctcttcgtc tccttcgtgg agcaggcttc cgacgaggac
    10021 ttgcgcaagc ccatcaggtt caagaacttc aagggcgagg accaggagcg gatcgtctgg
    10081 caaacggtct tccacgtctt caaccacttc acccaccacc ggggcgagat ctccggcgcc
    10141 ctggaccgga tgggcgtcgg caacgacttc gcaggcttct tccggtatct ctaggaggcg
    10201 acacccatgc tcgagccgcc gacgcccgcc gccatgcttt ccgcgttcaa ggaggccctc
    10261 tcccgccagc tcaaggccgc cctccacacc ctgggccgcg ccatggagac ctgccccgac
    10321 gccgaatggc agcaggagca cggcgacgcg cccttctccc aggtcgtctt ccacgcgctc
    10381 ttctacgccg acttctacct cggcagggac acccatccct tcaaggccca gcccttccac
    10441 aaggcccatg cggcgacctt cgccgactac gaggagctgg aggacaggaa gcccgtccgg
    10501 ctctacgaga aggccttttg ccgcgagtac ctggaacact gcctggccaa ggtcgacgcg
    10561 gcggtcggcg ccgagaccct ggagtcgctc gcgggcgagt cgggcatcga cttccggcgc
    10621 tgcggccgca tggagctcta catctacggc gagcgccaca tccagcacca cgccgcccag
    10681 ctgggcctcc gggtgcagca ggccaccgga caggagctga agtggtacag cggccactga
    10741 cgggcctcta tccccccggg ttgcccgaaa acggaaccgc tccgttcccg ggcgccgcat
    10801 aaagaaagaa ggtcgccacc ttcgtgacga ccttccgttt tcgcctcgcg atgcgcggcc
    10861 ccaggcgagc aacccggagg gaacacgtcg ccgaaggcga cgacgtccaa taaatttcct
    10921 ctatcccccc gggttgcccg aaaacggaac cgctccgttc ccgggcgccg cannnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgacgac cttccgtttt cgcctcgcga
    11101 tgcgcggccc caggcgagca acccggatag atgccaccgc gccgccaggc gcggacgaca
    11161 atttccttcc ttgcatctcc cgggttgccc gaaaacggaa ccgctccgtt cctgggcgcc
    11221 atataaagaa agaaggtcgc caccttcgtg acgaccttcc gttttcgggc aacccggatt
    11281 cgccttccgc tcggcgccgt cctggcgcct cgctccaggc cggggttctc gctttcggcg
    11341 ccatcgtggc gcctcacccg cggcgccctc gaagtctcgg gcgccgcggg ccgctcgaac
    11401 ccttcgaatc cgggttgccc gacaacccgc ctcgccgcag cgctttaaaa cgaaaaaggc
    11461 cggctcgtgt gagccgacct tagtcgggca acccggattc gaaccgggga ccccgagtcc
    11521 cccagactcg tacgctaacc atctgcgcta ttgcccgttt gctgccttgc ggagcaaaaa
    11581 aaccatacca tgtggcccaa ggacgggtca atagcttcgc ttcttttagt cggagggctt
    11641 cccggggccc gagaagacgg aaaggaggcg gcgggaggcc tcctccatgg ccgcggggag
    11701 gtcgtagtcg ccgttccgga ggcaccagtc gtagatgagg ccgcgggtga agcgcaggac
    11761 gaattccgtg acgtactccg cggagacgtc ggaggccagg aggcccttcg cctgctcctc
    11821 ggcgacgatc cggcgcagga cggagggaag gccgcgctcg ggggagatga agaagcgggt
    11881 cgcgttctcg atctgggcct tgtagacctg cctgatgagg tcaacgccga gctccgaggc
    11941 gtagcgggcc tggaagacgg cgaactcgac gaggcgctcg cggccttccg ccgccttgag
    12001 gcggcccgag acctcgtcct cgaagtagcg gtcgcatatg gaataaccct cgacgatgag
    12061 gtcggccttg ttgcggaagt ggtggtagaa ggagcccttg gctatgcccg cgcgggcgca
    12121 gatgcgctcc acggagacct cctcgtagcc ttcctcgccg atgagctgcc aggcggcctc
    12181 gtagatgcgg tccttggtct ccctggcctg gagctggcgg cggttcgggc tcgggctctc
    12241 gctgtccatg gcggccagga taccctcgaa cagggaaaaa atcagcttcg ccttgcgcgc
    12301 cgcccggccc cgtgctacct tgagccgatt tcgccgaccg cggtcggcaa aaacatatcg
    12361 attggcggac tccaccgcgc ggagcccgaa ggagcggcga tgtcggacaa ccagcaaagg
    12421 atcagggacc tgaccgggcg cttcggcacc tggtacgagg atcccgcatg gctcgccaag
    12481 ggccgcatga cgaaggagct ctacccctac gaccggatgt tctccccgat ccgggtgaac
    12541 aagctctcca tcaagaaccg cctcgtgatg gcgcccatgg gcaacatcag catggcggag
    12601 gagacgggcc ggcccaacgc gaagatgatc gagtacttcg cggcgcgggc ccgcggcggc
    12661 gtcggcctca tcacctcggg cctcgtcccg gtgagccacg ggatcgaccc gagcgtcacc
    12721 gagctcgggg agctctccta cttcccccgc atcgacaaga gccgcacggt gttctcaggc
    12781 tggcgcgacg tcgccgagcg ctgccgggcc tacggggccc gcttcttcat ccagctcacg
    12841 ccgggcctgg gccgcgtcgg ctccccggag tgcctggtca acaagctgaa gcttcccgtc
    12901 tccgcctcct tcaaccccaa cttctacatg cccgccctgc cctgccgccc cctcacggac
    12961 gcccaggccc ggcgcatcgt caagaacgcg ggccaggccg ccgcggacgc gaaggcggcg
    13021 accatcgacg gcgtctacct ccacggccac gagggctacc tcctggagca gatgacgaac
    13081 cccgccttca accgccgcaa gctcggcgcc ttccgcgact ggaaggcctt cggcctggag
    13141 ctcgtgcggg agatccggcg ccggaccggc cccgactacc cgatcatgta ccgcatcgac
    13201 ctctcgctcg ccctcaacga gacctacggg aagcggatgg acagcgtgag gagcctgcgc
    13261 aagttcaaga aggagcggac ggtcgaggag accctcgagt acatggaggc cctggtccgc
    13321 gagggcgtcg acctcttcga cgtggacctg ggctgctacg acaactggtg gctcccccac
    13381 cccgcgggcc ccatggcccc cggctgcttc ctggaggtgg cgcgcatcgc gaaggagcgc
    13441 ttcgccgagc gcggcgtcag gtccaacgcc ggcctccccg tgcccgtcgt cgccgtcggc
    13501 aagctcggct atcccgacct ggcggaggcc gcgctccgcg acggctcctg cgacatggtg
    13561 atgctagggc ggcccctcct cgccgacccc gagtggccga acaaggcctt cgccggagag
    13621 gtggaggcga tctgcccctg catcggcgac caggagggct gcatcaacga gttcgtggag
    13681 ggcggccacc cgcagtgcgc cgtcaacccg cgcaccggct tcgaggagct ctacgacggc
    13741 cgcgagctcg cccccgcggc gaaggcccgc aaggtggccg tcgtcggcgc ggggcccgcg
    13801 ggcgtcacgg cggccctggt cgcggcgcgc cgcggccacg aggtcgtcct ctacgacgcg
    13861 cggaaggaga tcggcggcat gctcgtcccg ggctccaggc ccgcgatcaa gtacgacatc
    13921 gcgaactacc tctcgcacct caaggcggaa ctcgccaagg ccgaggcctc gggccgcctc
    13981 aggctcgagc tcgggaaggc gcccgacgcc gccgccctca aggccggcgg ctatgacgtc
    14041 atcctcgcct ccacgggctc ccggcagacg cggccccccg tccccggcat cgagtccccc
    14101 aacgtcgtcc acgcggtcga cctcctccgc gaccctgccc tcctgggcgc cgcgaagagc
    14161 gcggtcgtcg tgggcggcgg cgtcgtcggc tgcgagaccg cctactggct cgcccacgag
    14221 aagggcgtga agaccgcggt cgtggagatg ctgcccgaga tcatgaaggg cgtctgcacg
    14281 gcgaaccgcg gctggctcat ccactacctg gaggagcggg gcgtgccgct cctcaattgc
    14341 gcgcgcctcg cggaggtccg ccccggcgag gccgtggtgc ggcgcaacgt ctcgaagacg
    14401 gtgcccgacc ccctcgtcac ctggacgccc ctcctgccgg agaacatcga gaaccccttc
    14461 gcgaagaagc tcaaggtgga ggaggtcgag gagcgccttc ccgccgacct cgtggtcctc
    14521 gcggcgggcg cccggcccga cgacaccctc taccgcgccc tggtcgaggc ccgggccgcc
    14581 ccggaggtcc ggagcctggg cgacgccttc cacctgggac gcgtcttcga ggcgaccaag
    14641 gcggcctacg cggtcgccac ctccctgtag cccgcggcnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnncc cgaaggcccc gcgacgagcg caagcgcgcc cgtcgcgggg
    14821 ccttccctta tccgcgcggc ccggcccttc cgtccgcccc ggccgccgcc ttccccgccc
    14881 gcggcctcgc gaggcgcagg tagaggggcg agagggccag ggccgcggcg aggaggaggg
    14941 cgcaaaggag gtagccgcgc gaaagcgagg cctccgccgc gcggccgacg ccgaggttgg
    15001 cggcggccgc gacgccctcc acgaccatcg cgttgagcga cagggcggta gccctctcgc
    15061 ccgtccccac gagctcgttc tggacggcct ggaagtaggg cgcgaagagg gcggccgagg
    15121 ccgagacgcc ggccagggcc aggagggaga cggccgcgcg cgcgggggct aggaggagga
    15181 cgaggacgga gaggagctcc gtcgcgaggc aggagaggat gacggcccgg cggtcgaagc
    15241 gccgcgagag ggctcccgcc gcgcccgcga cgacaggcac gagctggagg gccgcgaaga
    15301 tgaggccgaa ccaggccctc gggatgccgc tccgctcgta ctgaagctgg cccaggaaga
    15361 cgacgaggga gcgcgagacc tccgcggcca gggcccccga ggcgaggagg agaaggaggc
    15421 gccgatcgga aagaagccgc ttcagggagg agaggacgaa gcggcgctcc cgaggcggcg
    15481 gcgctccctc gtccgaaagg aggaaggaga gggcggccgc ggccgagtag ggcaggatcg
    15541 tcgcgtaggc cgtccagcgc agcgagacgc cgtagaggag gggcgagagg agggaggcca
    15601 ggacgatgcc cgccgtcgcc gccgcggact gcaggccgaa gttccgctcg gcctcgccct
    15661 cccgcgagga gcggtagagg agggccgagt cgcagccgga gatcgcggcc acggcgacgg
    15721 agaggacgag gcgctcggcg aggaagccgc cgaagccgct cgcgccggcg aagacgagct
    15781 tggaggcggc gaagaggagg ttgccggcga cgagggttcg gcggtagccg aagcggtccg
    15841 cgaaccagcc ccagggcgcc tccaggagga gcatgaggat ccaggagacg gactcgatga
    15901 ggaagagctc gtccagggcc aggccgtagg cccggcggta gagggtggcg acgggcccgt
    15961 agaagacgaa gccctgcagg aacgagacgg ctatgagcag cctgacgttg cgtgtcgagt
    16021 tcatgggatg cctttccagt cgtatggtgt gggggcgacg ggaaggggct cgctagatcg
    16081 gctggacgct cggaggcgcg ccgggcggaa cgccggcctc gggcgaggcg aggaggcggc
    16141 cgcactcgcg cgccaggcgg gcggggtccc agggcttgcg gaagaaggcg tccagggcgc
    16201 ccgagtcgcg aaggcgctcg agctggtcct cgtccgcgtg gccggagacc atgatcgtct
    16261 tgacctcggg atgcccggcg cggacgttcg ccaggaactc gtccccgcgc atgccgggca
    16321 tgagccagtc cgagatgacg aggacgacgc tcacgccgtc ggcggcgagc tcctcgatgg
    16381 ccttgaggcc ctcgtccgcg tcgcgcgcga tctcgtatcg gtaggcgggc ccgagcttgg
    16441 cccggagctc ctggcggagg ctgaggagga tgatcatctc gtcgtcgacc atgagtatgg
    16501 ctctatcgat cttcatcgcg cgctcgcctc gggcccgtag ttcctagcca cgggcagacg
    16561 cacgcggaac accgtgcggc ccggtcggct ctcgaatgat atatcgccgc cgttggcccg
    16621 cgcaagcctc ctcgcgatgt ccagcccgag tcccgtgcct tcgccggggg ccttcgtgct
    16681 gaagaagggc tcgaagatcc ggccgcgcag gccctcggga atgcccggtc ccgagtcggc
    16741 gaaggacacc accgccaggc caccctcgat ggccgaggag acctcgaccg aaccctcgtc
    16801 gccgacggcg tggagcgcgt tcgaaaggag gttgaaccag atcctgtcga gctcctccgg
    16861 ccttcccagg acggcctcgc aggccgcgaa gtcgaagcgg aggcggacgc cccgcctcgc
    16921 cctggccttg aacagctcgg ccatcgcgcc caccgccccg gccaggccga cgggccgcgg
    16981 actctcgccg ggacctcctc gcacgtaggc cctgagcgcc gagaggaccc ttgcggcctg
    17041 ctccacggag acctccgcga cgcgcccggc cctgcggccg ccgacgacgg cgcgcagggc
    17101 ggcgaggagg gcctccccgc cggggccggc caggaagggg gaaaggcgcg ggacggcgcg
    17161 cccgaggccg agctcgacga gctcgtcgcc cagggcctcg gcttcctcgt cggccaagcc
    17221 cgcggccgag agctccgcgc gggctgcccg cttcgcccgc cgctcggcct ggggatcgac
    17281 ctcggggctt tcctcgtccc cgagcccgcg cgcctccccg aggagggcga ggccctccgg
    17341 cccgagccgc gagacggagg cggccagctc ctcgaagccg ggccccaggg aggcccggac
    17401 cgtcctcgct gcggcggcta tcgccgcgag cggcgtgttg agctcgtggg ccaggccggc
    17461 cgatatgcgt ccgagggcgg cgagcttctc ggaggcgagg gcctgggcct gggcggcctt
    17521 gagctcctcc atcgcccggg ccaggccctc gttggccgcg gccaggccct cgttcgcgcg
    17581 gacgagctca tccgtccgag ccgccacctt gcgctcgagc tcagcgttga gcgcctccag
    17641 ctcgtcgcgc gcccgcgcga gctccgtgac gtcgactacc gcggagacgt aggcggagcc
    17701 gccttcgaac tcgaggcgcc tgaccgacac gatgctgctc gcgaccgagc cgtcggctcg
    17761 gcgggagcgg tagggcatcc cgtcgatctt cccggaccgc gcgatctcgg cctgcatggc
    17821 ggccgtctcg cggggatcga cggcccggct cacctcggag acaggcttcc ccaggatgtc
    17881 ctccagccga tccgcgccca cggactcgag gtaggcccgg ttcgccgcca ggatcacgcc
    17941 gggcccgccc cgctggatga ctatcgggta gggggccgcc tccaggacgg cccgcagggc
    18001 cgcctcgcgc ccggccagcc cggactcggc gcgcttccgc tcggcgatct cgaagcccag
    18061 ggagaggacc gtcgccatgg tctcggcgag gcgctcgtcc tccgcggtcc agtcgcggct
    18121 ctcgccgacc gcctcgaagc tcaggatgcc ccgcacggcc tcgcccatcc agacgggggc
    18181 gtccaggagg gagcggatgt ccttggccga gaggtagcct gagggcaggg ccgaggcgac
    18241 gggatccccg cgcacctccc gcgccaccac ggagagtcct cggcgatggc gcgagacgta
    18301 ctcgggaaag cccgcggtcg ggagcctctc gcccgacctg tgccgccccg aggcgcggtc
    18361 gtagaaatcg acgcactcga tctcgccgta gtccgccgag tagcgccaca cgctgacccg
    18421 gcccgcgccg acgtagcgcg agcaggcctc cgtcaccgcg cgcactgcct ccccgaaatc
    18481 gcccaggaag aggcggccgc tcgcaaggag ctcctggagg gcctcgcccc ttgcgcggac
    18541 gcggtccgag acgtcgatga agctcgagag gatccggccc cccgagacgg aggcggagat
    18601 ctggaagacc cggtcgcggc cgtccttgcg gcggaccctg aattccccgg gctcgacgaa
    18661 gccccccgtc cccgaagctc cggcgaccgc ggcggaccag gcggcggcgg tctccgcgcg
    18721 gtaggccgga tcggggtagg ccgcgcggaa ccagctttcc acggtcggca ggtcctcgag
    18781 ggcgtagccg agctcctcgg cgaagcggcg gttcacggcg aggatcctcc ccccggcgtc
    18841 cgagaggcag agggccacgg gggcgagctc gaagaaatcc ctgtcggtcg cgctaccaag
    18901 tatgggagcg gccggaaccg gggtcaaatc cggcgggcgg ccggccgacg aggccgcggc
    18961 gccgccgggc aggcagggcc ggccctagcc gcgcgcgggg gcgggggcta gaatggcgcg
    19021 gacggaggct cttcgtggac ggcggcgcgg gcgggacatc tttcggcgcg aagtatttcc
    19081 cggccctcgg gaagcgcaac ttcaggatct tctggctcgg gcagtgcgtc tccctggtcg
    19141 gtacctggat gcagaacgtg gggcagtcct ggctcgtcct cgagctcacg ggctctcccg
    19201 cgaagctcgg cctggtctcc gccctgcagt tcctcccgat gatgctcttc tccctcgtcg
    19261 cgggaccctt cgtggaccgc ttccccaagc gcaagaccct cgtagccacc cagggggccc
    19321 tcctcgtcct ggcgctggcc ctcgccaccc tcacggccac gggcctcgtc cgctactgga
    19381 tggtcctcgt cctggccttc ctcctgggca tggtgaacct cgtggacgtg ccgacgcggc
    19441 agtccttcgt gatcgagctc gcgggcaggg agagcctcgt gaacgcggtg tcgctcaact
    19501 cggcagcctt caacctggcg cgcatcttcg gccccgccgc cgccggactc ctcatggagg
    19561 cgatcggcat cgcgccctgc ttctacctga acgccctctc cttcgtcgcg gtgatcgccg
    19621 ccctcctcgc gatcgacgcg ccgcccctgg tcggcgagtc ccggacgggc agcgtccggg
    19681 aggtcctggc gagcgtccgg gaagggctct cgtacgtcag gggaaagcgg gagatcgccc
    19741 tgccactcgt cctcatggcc ctgatctcca ccttcgtgat caactacaac atctacgtgc
    19801 ccaccttcgc ccgcggcccc ctgggacgcg gagcctcggg ctacggcttc ctcatgacgg
    19861 cgatgggcgc tgggtcactg gccgccgccc tcctcctcgc gacgaggagc ggcgcggccg
    19921 ccgcgaaggg caagggcacg gcgccgggcg ccgcgcggct cttcgccgga gccatcggca
    19981 tgggcgcctg cctggccctc tgtggcctgc agcggagcta cgccctgacg gccgccctcc
    20041 tggccctcac gggcttctgc acgatcgtct tcaccgcctc cgcgaacgcc ggcgtccagc
    20101 tcgcctccga cgacgcccac cgcggccgcg tcatgagcgt ctacagcctg gtcttcggcg
    20161 gcgtcacgcc cgtcgggtcc ctgtacgcgg gctccctcac cgacgcggcc ggcccggccg
    20221 ccgcgatggc cgcgagcgga gccgtcggcc tcgccgcgtc gctcgccatc gggctcgcgg
    20281 cccgccggcg ccgcgacgcc cgccggaccg gtccctgagc cgcccgggcg ccgccctctg
    20341 agccgcccgg gcgccgcccg aagctctcac cgccgagccg ccccaagcgc cgcccgctta
    20401 gccgccctga ccgccgcccg ccgatccggc ccccgacaga cgccaacaag gtcgcccatc
    20461 gcgccggatc gcccatcgcg cttgaattcc ggccaagacg cgctatcctg tccgtagcta
    20521 aacaagcacg gaggaaccat gaaacacagg atgctcgtcc tcgcagccgt ggcggccctc
    20581 gcggccgcct ccctcgcctt cgcggcgccg cccgcccagg ccaaggccgt gccggtctat
    20641 cccggcgccg ccgccgatcc ggccgccgcg gactacgcga aggagctctc caacggcgaa
    20701 ggcgaccccg atctcgtctc cgaggaggtc atcgcctacg ccaccgccgc cgccccggag
    20761 gaggtcttcg cctggtaccg gcagaagctc gccgccaagc cgatgcgcga catcacccag
    20821 gccgagtacg agagcggctc gaccccgccc atgtactacc tcgagcccta cgaggaagag
    20881 gacttccgcg acggcaccac gggcgaccgc aaggtcgtcg tcaagtacga gggcgcctgg
    20941 atcagggagc agctcaaggc caaaaggaag cccctggagg gcgcctacct ctccagcggc
    21001 agcttcagct ggatgctccg cgagaaggac ggacgcgcgc ggggcatcgt cgtgaccgtc
    21061 ctggacgcga gcttcctgcc ctacatcggc gagggccccg gagcgggccc ggccacgctc
    21121 ggcaagaaga actacgccca gaagacctac gtcttcgtct actcccagct ccgcaagtct
    21181 gaaggcgagg ccggggccga gcgcgaggaa cgcagggacg ccgagggcga ggctgacatg
    21241 gaggcggccc gcgcctactt cgccgccaat cccccgagcg cgaaggagct cggcctcccc
    21301 ctctacccgg gcgcggcctt cgacgccgag aactccgctg gcatgagcat ggacgagggc
    21361 atgaaggcct ggctcttcac gaccgccgac tccctcgaca aggtcgtcgc cttctacgag
    21421 aaagcctcgg gcaggaaggc cgagcgtctc ggccaggcca ggcgcatcgt cctcaagggc
    21481 tcgggcctga tgccggagca cttcctggcc ctggagcccg gcgcggccgg cgggacggcc
    21541 atctccttct tcaagaccga ggagtgacct tgcgcccggg ctcggcgcgg gcgccgcgcg
    21601 ggcctgccgc cgcgatggcc gcgagcggcg ccgtcggcct cgcctccgnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgaagctgcc cgcggccgcc gcgacgtccc
    21781 cgccgcccgg gtccccgcgc aggaagaagt cctcgaggct ccccgcctcc atgagggccc
    21841 gcctcgcccc tcgggccagg gcgatcatgt ccttgggaaa gcagtagcgg tactggaagc
    21901 cgccccaggc ctcgcgcagg ccgctttcgc ccatcgcggc cagggcggag gcgggcctct
    21961 cccccagggg ctccagggcc gcccggacgg cgcggaggat gaggtccacg gagccgaagg
    22021 ccagggtcga ggcgaagagg cccgcgacct cgcggtcccg cggatcgtcg aaggggacga
    22081 ccagggccag gggatcccgg cggaggcggg gccccgcggc gcaggcttcg tagacttcct
    22141 ccaggaaggc ggctatagcg gggtcgtagg cggacgcctc gggcccggcc ccgcggcgcg
    22201 cgccgcggac ctgcccggcc gcgcgggcgt cgcccgcccc gcctgcctcg cgggccgaag
    22261 cgcgcgcgcc cgcgggccgc ggactccggc cgcccgcgga agtccggctc acggcgcgga
    22321 ggcctcgagc tcccgcgacc gacgcttgag ctcggggtcg ccgggcgaaa gggccagggc
    22381 ctgcttcagg tagaagcgcg ccttctggaa gtcgcgccgg cgcaggtata tgtcgcacat
    22441 cgcggccaag gcctccgagt tgtcggggtt ctccaccagg gccgagcgca gcgcggagag
    22501 ggccgcgtcg tcggacttgg cgagctgggc ctggagccag aagagcgagg agcggaaggc
    22561 cggcgagccc ttgcccgcga ggagcctcgc cgcgaggtcg gaggcggcct tctcggcctt
    22621 cgtccccagg agggcccgcg cgtaggcctc ggccgccgcc tcggactcgg gcctctcctt
    22681 gtaccaggcg gcggcctcct ccagggctcc ggcccagtcg ccgctcctgc gcctgaccag
    22741 gccggagagg gcgcggtcgt cgaagcccgg cgcggccttc gcgcgcggga ggtaggaggc
    22801 cgcgagggcc cagtcgtagg aggccgccgc gtccaggagg aggaggcgcg cggcctccgc
    22861 cctggcggcc gcccggagcg attcctccaa gggggagagt gccgcggcgc cgnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcctgccc ctcggcgcgc ctcgcgagct
    23041 cgcggccctc cgagcgggcc gcctcgacga ggtccgcccg cagcgtggaa gggacggcga
    23101 agaggaggcg ggccgcgacg gccaggagct ccgggtcgtc gggataggcc gcgaggccgc
    23161 gcctggccca cttcagggcc tcgtcgcggc cgcgcaggcc ctcggccacc tcggcccgta
    23221 gcgcgaggta gcggcggttc gaagggtcga cggtgccgta ggcgtccagg aggggctggg
    23281 cctgctggaa ggacctggag cgggccagga ggtgggccct gacgaggacg aaggccgagt
    23341 cctggggatc ctcgaggagg atgcgcagga ccaggggctc cgcctcggag aggcggccca
    23401 ggtcgtagag ggcccgggcc cggacgcggc ggatcggtgg cgagtcggcc gcggccgggt
    23461 cgacctgcct gaggagctcc tccgccgcgg ccggccggcc cagggccagg taggccctcg
    23521 ccgcgcccag ggaggccggc caggcgtcgg gagcggccga gcggacgccc gcgtagtagt
    23581 cgagggcccg ggcgaagtcc ttgcgccgct ccgcgtccag gccccggacc aggccaggca
    23641 ggacggaggg cgcgcccagc cgctcgaagc ggtccagggc ctccaggcag cggcgggccg
    23701 tctcgcggga gtcggagcgg aaggccgcga gggccggcac gagctctccc aggggagtgc
    23761 cctccgcctc gggaaggagg gccggcacgc ggcccgccgc ggcgtcggcc aggctcctcg
    23821 cgagcggcga ggcgggcagc tcggcctggc cgcgcaggtc ggcgggcacg aaggagtccg
    23881 cgctctcggg atagacgagg agggcgatcc tcgcgcccat ccaggcgagg ctaaaggctt
    23941 cctcctcctt gagggacgtg gcctcggcga tgagggagaa ggccttcgct atggaggagg
    24001 gcgagccgaa gacgacggcg gcctccacgg cgcggcgctc ctccagcgcg cgggagaggg
    24061 cctggggcgc cgcgcccttg agggccgtgc cgccgccggc cgcgccgggc tcggagccgg
    24121 gctggggcga ggaggccggc gccgccgggg cggcgggcgc gccggcgcag gaaaggcagg
    24181 aaaggaggat ggagaggagg agggggacga gggcggcggg gcgctccgcg ccccggccgc
    24241 ggcgcatcac ggccgccctg ggcggccctt gcggccgcgc ttgcggccgc tcgccccgta
    24301 ggccgcgaag agggccgcgc cgcccgcgat gaggacggtc ggccaccact ccgagatgaa
    24361 ctgcctgaag ccgacgcgga ccaggtcgaa ggagaagagg cagaagaaga agccgaggaa
    24421 gacgaagatg agggcgggca ccgcgccctg gggcctgagg cggcggaagc ggcgcaggcc
    24481 ggccgcgagc caggacagcc ccgcggcggc catgaggagg ggccaggcgc gctcgaaggt
    24541 ccagcccgcg aagactcccg ctatcatgag gaggcccgag agggagaaga ggacgcccgc
    24601 gaagaagaag ggatccgaga agcggcgcac gatggccagg tagaggagga ggccgccggc
    24661 cgcgaggacc atgaggggcc agaagcgctc ggcctccggc gcgatgcccg tcgtcctgag
    24721 gaggaagccg acgcccgcga tgacggcggc cagggccccg atgaagagaa ggtcgtcgtt
    24781 gcgggagcgg ctcgggctca tcaggcttac gattctagcg gaatccgccg atcttgccaa
    24841 catgaaagag cgcgtgctat gctccgcgga catggcgaaa ccgcgttccg gcgcgccggc
    24901 gcgcccccga tccgccgagc gggccctcct cgcagcggcc ttcgccgccg cggccctcct
    24961 cgggtcggcc tgctcctctc cgcgcgcggc ctacctctcg ggcggccgcc aggacagggc
    25021 cgagctcgcc gagctcttcg gcctcctcga ctcccagtcg gccgaccagg tggagcgctt
    25081 cgcggccgtc aggcaggtct cctcgatcct cctcaggcag aaggagtacg ggcgcctcac
    25141 cgccctcctc acctccatcg ccgcggagcg gccccaggat ccctacgcgg cctggtacct
    25201 cttcgccgcc gcctacgcct acgaccagcg cggctcggcg ccgatcgccg ccctctacta
    25261 cgaccgcatc gtcaagaact accccgatct ggaagtggac gggaagtcga tccactacga
    25321 gtgcctcctc cgcctcatcg agaccgagga ggctcccgag cgccgcatcg agtactacaa
    25381 ggacctgatc gcgcgcttcc ccgggcgcgt ggacatgggc cgcatcctct tcctcctcgg
    25441 gaaggagtac gagcgcgtgg gcgactggga actcgcggtc aagacctacg cgaagttcct
    25501 gccctacttc ggcgcctcca tccccggcta tcccgacgcc ttccagtacg cgcgcaacgt
    25561 catcgagttc tacaacagcc ccaaggactg ggcctacgag gacctgcaga ccctcgtcgg
    25621 gaacatcaag gcggccctcc aggccggcga cccctaccgg ctccgccgct accgggccaa
    25681 ggtcaacttc ttcgcgatgt cctggcacca ggacgagacc gacgtcaaca gccaggtcct
    25741 cttcgacttc tcggagttca tgtccggcgg ccgcgtctac tcggcggagg ccatggacgc
    25801 gagctccggc ccccgcgagg cctacctcaa gacctggggc tggtccgagc gcatctccac
    25861 ctggtacctc tatttccgga agatctactt ccccgccgac cccgagatcc acg
//
LOCUS       DAOOSJ010000007        24896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1400_length_24896_cov_6.354092, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000007 DAOOSJ010000000
VERSION     DAOOSJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 24896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24896
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_24896_cov_6.354092"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLG14_00865"
     CDS             <1..635
                     /locus_tag="PLG14_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47706.1"
                     /translation="AAVATLVIIPMQIAVFAAFPIPTTVEAWFAQFAANPLVGLFHAD
                     FFIMVDNILIAVIYLAFYHSLKETDKGFLQVAVVLGLVGIAAYISSNRTFELLSLSRK
                     YAEAASGSERLVLLGAGQAMLAGWQGTAFDAYYVLNGVCLLIVASLMWKGGPYGKTTA
                     AFGLAAGVLMTIPSTAGTLGLVFSLLSLLPWYVFSIRFGLVFRRLARREA"
     gene            638..1030
                     /locus_tag="PLG14_00870"
     CDS             638..1030
                     /locus_tag="PLG14_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOX47707.1"
                     /translation="MDGEFGTIDEYLAGFSGRTRELLEELRAAIRAAAPEATERISYR
                     MPTFFLEGNLVHFAAFKGHIGFYPTPSGIEGFEAELAKYESGKGSVRFPLDEPLPLDL
                     VRRIVLRRVEENLEKAAFRRAKKRNQGR"
     gene            1038..1688
                     /locus_tag="PLG14_00875"
     CDS             1038..1688
                     /locus_tag="PLG14_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47708.1"
                     /translation="MRALAVRLARLLLGLFLYALGIAATMKANIGYAPWEVFHAGVGK
                     AIGMSIGNVSILTGLAIVLVAFLLGEKLGLGTVLNMLLIGVFLDAILSLGLIPRMETW
                     IPGLAMLAAGLFTISLASFFYIGSGFGAGPRDSLMVALRRKTGLPIGLCRGGIELAAV
                     VVGWLLGGMVGLGTVVSAFAIGLCVQLTFRVLRFDPTKVRHETLGATFGMAPRRPR"
     gene            complement(1776..3593)
                     /locus_tag="PLG14_00880"
     CDS             complement(1776..3593)
                     /locus_tag="PLG14_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47709.1"
                     /translation="MNPTRTRELALAIASFPSVTGSEGETRFAGFLRDLLADWPYFRS
                     HPEHLRLIKTLDDGRERYVLAAAAVARGAGVRPARAPIPTVILTGHYDVVSTANYGPL
                     EPYAFDPEALSPRLAAALEDEARAASLRGESLGETESRALRDLSSGDFLPGRGLLDMK
                     AGLAAGLALLEDFAAQAEAEAASRGASAGAGPGAGPDRTGGLADDAPGALLFLAVPDE
                     EGSSHGMLSAVRALPGLLSEWGLAARAAINLDAAVDQGEGEAGRAVFLGSVGKLHPFV
                     LFLGRPSHAGAPFDGVNPALLAAEYARRIECADLFGAGSASSDDPGGAAAGTPATEAR
                     SSEPPSPPTILYYRELRDRYDVTTPESVFVSANLLTHDRGPASCLEAVSRLAGEAMEA
                     ALGLLRGRAAAFAEASGWKSALPDRTPRVLGWAELEELARAAGAAAWEAARARAAKAA
                     DAGDAMGALMGLVSEAVRAAGLEGPAAVVGLAPPYYPRSRLDSVRDGRLLGFVEKALE
                     AAEARGLGRTTSRPFFPGISDMSFLYPEDDASERLLASAACPLGLDPLPAGFSCPALN
                     LGPWGREYHQRLERVHASYAFGILPELLRLVAEGILAGK"
     gene            complement(3655..5079)
                     /locus_tag="PLG14_00885"
     CDS             complement(3655..5079)
                     /locus_tag="PLG14_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX47710.1"
                     /translation="MGKIIRNRGLLGPGNFYREALAIVVPVMLQSLVTGLVSLVDNFM
                     VAALGDSKMAGVNVANQINFVYLVIVNTVCTAGGIYLSQHRGAGDEEGMKQAFRFKLL
                     MATLISAAHLLVCLVFPEALIRLMLGGNAEGAQIVAEGARYMRAVAASFIPIGFASAM
                     SSSFRDTGVTKVPLLVSTSAALVNTFFNWVLIYGNLGAPRLEVAGAAIATVLARLVEA
                     AAFAAMIARKRPAFAFSPRRLLSVDRRLFGAILSRSGMMLFSETAWVISETIITALYN
                     GRGGAETVAGMAAGWTIANLIFLVFPAIHAATGVIVGATLGAGRLEEGKLKAGWIMSG
                     SAVFGAFAGAAAAAATLAIPLVFGNLSGDARAVTRGLVFVIGSYLPLWCFLNAQFAVS
                     RAGGDTLMGVWADVGVTYAVFIPAALAIARFTAWGPVALFGVAKLSDIPKALVAGWWL
                     KKERWVRNLAASPEAPTPGAPPTP"
     gene            complement(5143..6696)
                     /locus_tag="PLG14_00890"
     CDS             complement(5143..6696)
                     /locus_tag="PLG14_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47711.1"
                     /translation="MPSALATFLLLLAPVAAMLFLNLRLSRRLSYPHELLEEERRRGP
                     AAFLFRALRAYWDLVLDAALALAVALALAGGGAPRDGAEPRGRPALVVDCSRSMLAGR
                     PGSRPLDLAMRRLAEDESLRKAEPFALAFDPRTARTRLVPLRRLLSGVEGGGAAARLE
                     GELAFLAVDYGELAALRRRGFGRITLLTDALPFRTEGFEAVELGFPDPASGESEFAAY
                     PSSARHDRASGSWLVALVEAGRRGDLSVKAWRAPSGPFERLAPSRYGVEEGPAGRILR
                     FSSPGLYLVSLPGPYGEAGADFALELASPATAASAEGPFSSRVLAAFPYLAASEKPEL
                     ALVDEGGKGAGAAARAGGPRLVSTRLAPEDGRLVLSPALAGGRLIAAGAEPEAPGFAP
                     APASGGRARARRFVLGPAALANEDLPLAYDGAILAGAEPPFLDSTRPGEGRIEAAMGA
                     YLLRSGDSLRPLAPPPSEYFAPARDGVLRLPPPPPPRLLWSAVLGLLSAAKLLAWRKS
                     TGKRLLARG"
     gene            complement(6689..7582)
                     /locus_tag="PLG14_00895"
     CDS             complement(6689..7582)
                     /locus_tag="PLG14_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX47712.1"
                     /translation="MSDGEAARSGQPRDAARGLVDPEAAALVRAFGRRTPRRPPLSAQ
                     ASRDPMFRRAGSSFDLKNIREYQSTDDPRRIEWKLEGRTDRLFVKEYYDEERDGAAVL
                     VDLSASLGVYGAAEAKRVGATIAWMLGALGVPTSLEAFAGRTLRRLSRQRGGKSTAPI
                     ASFFAGLEPEGRTDISAAVASARAASRYRRLVLVSDFFDRDWSPRSSPFARNCFVRLY
                     RPFSDLRPGRAEVDVEDPETGRRLRLPWDAAAEAAYARREAELDAAFREAARRGAYYG
                     ILRPGEPRRTLYWSLLEALYA"
     gene            complement(7579..8619)
                     /locus_tag="PLG14_00900"
     CDS             complement(7579..8619)
                     /locus_tag="PLG14_00900"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX47713.1"
                     /translation="MGRNNDIAMGDYGAGELEADLRAVADFAKSYHAYLESRVVGCHD
                     IVERLFIAFLCEGNVLLEGAPGLGKTSLAKAWAEFLGLGFKRVQFTPDLMPLDVIGSS
                     VLQTAAGGERAFKFYEGPIFSNVVLGDEINRATPKTQSAFLEAMEEKRVTFLGETREL
                     PSPFFVIATQNPIELEGTYPLPEAQVDRFLMRLFFAMPDFQALRSILDASERPGLGAP
                     AGREATLAALAAFRRASRALAVPEEVKSYVARLVAQTQPGTSGVEAARKYLRYGASPR
                     CALGLFRAARARAVAAGRPNLAFEDVEALFFDLANHRVILDFEAEADGVTAEEILAAV
                     LAAVRREYRGAA"
     gene            complement(8606..9301)
                     /locus_tag="PLG14_00905"
     CDS             complement(8606..9301)
                     /locus_tag="PLG14_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47714.1"
                     /translation="MRPFRPRQAVLEAGLAAVSGFLLYLAMLVLEGLGDLLFAPGSPE
                     AARRALFALIEEGAKLGLLLAFGLRFGAGGPRRRGADALRLGAARGLSLGIAAVAVFA
                     AIENLAYFAASPGWGAAGRLLWSEPVHLVSALALSLGLFPLVSGRGGPAGRSAARAAP
                     AIAGGFALAYAWHLAANLAASGLLLPRAAEAPGGAARLLAAGSILNAAALWALVRLYL
                     HKAVIGGFLYGKE"
     gene            complement(9298..10431)
                     /locus_tag="PLG14_00910"
     CDS             complement(9298..10431)
                     /locus_tag="PLG14_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47715.1"
                     /translation="MSRPAGPAAERALGGRLLRSCRSALLARLAAWLCASAIAWSAGN
                     AAFLLAGAPEAAAMGLLILLASLLPAFLAWPIPEESLRAALRVSDRDTTFEAWLEAPP
                     GPARDLLELRAAARAAELELETPPRLKPFAGLKPLAASALAALALFELLALVAVGRPL
                     LSYAPPPAAGRGSRIEEGGFARPGEADPASLSAPREGMEEAKAEGSPETQAASREARE
                     AASEEERARALYESKEKALEEDLALGDAAQKSAEKRAGSGGSEGAGPEGQKAAAPMAK
                     PAEGRGTERARGDRPAQAGFEAADESLAPSPLLDYRARFARVYAERTRDAVEASGELS
                     LGRLRDYERRYFESFALSAGIAPREEAYAALLKRRWRELGGKP"
     gene            complement(10428..10907)
                     /locus_tag="PLG14_00915"
     CDS             complement(10428..10907)
                     /locus_tag="PLG14_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47716.1"
                     /translation="MEATRFAKRLLFFLPLLGAAAAINPYSGASAWLGPGAAFFAVAT
                     YARLGALALGRRGRDEALLALECLPFLAYGAACLGAAEPAGLALFLLSSLALWAFLLS
                     LEDLLAPKAIGFSLVLADAAAFALAAAALDRLPPAATVGGGAACLALSRYAAARRRP"
     gene            complement(10894..12018)
                     /locus_tag="PLG14_00920"
     CDS             complement(10894..12018)
                     /locus_tag="PLG14_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47717.1"
                     /translation="MAASRLAAFLLAALALAALAGAQEPANARGSSAPPQGAFQAAAA
                     APRAAPIAIQGSPGLRASGGPFLPYRFSRIAAEAEAGFLGPFRVFGLGWDGGERLLFE
                     AQAGAYSGVVEAVFHLDEETRALRVEADSPAGPLRLELDLAAEERRTGGADQGERSRR
                     ERMRALLPPPALTPRSVPLERLTLSEATADEAGRAAAANFSPPRPVAALALLAAFALL
                     AAFAEAGAFRRRAARGEAARVEAARGGTARGKAGIGLVCAASLAAALGAYALLPRRAL
                     LFSAALPAAARGSAYSGSLELSRESGRGFQALRYGDPGRGLELVRIVTPGWSSVPLSL
                     VASDKDDCRFSEPPLVVFREGAYALRSGSPLSGWVLHGSD"
     gene            complement(12063..14300)
                     /locus_tag="PLG14_00925"
     CDS             complement(12063..14300)
                     /locus_tag="PLG14_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX47718.1"
                     /translation="MRRNKWDAAKCAATALPFLFLAAYLAHAALPLPAFLLSERRYVA
                     LAKGAGAEALAAARGGAREAFALLPGRLLPLPPRGSPASAYEQAAALLPDRPSLEPAI
                     ADIEAGLPALRRARLVAGRLPAAGELLRLQGAASLAALPLALEVARDEASPLLLALSH
                     RIAPNEERRAFELLFAPEARSRAAIEVEAGGTALWRGNGAELPADLVLRLSAPADRLA
                     EGLRVRASEGGREESLRLDLGAESSEAPRVLVVSAKGADKRSFIERIYPAERATPEAA
                     AAKDLHGYELVVVDGLPLGGLGGGLRESLLDVARRRTGSILFVADSPDFGKKGDDPEL
                     EALLPASLAPKSLKDLPDLAILILIDTSGSMFGDKLSLAKVTGLELLRNLKPSDRVGM
                     LLFSDERRWVYDFVEAGGVSAAPVLEPLRAKGGTDLAAALSEGLDRASLQGLKDKHVV
                     LVTDGVTKPADFQALADRARALGATISAMGVGDDADQALLQRLAYRGGGRYYPVASPD
                     EVPALIFEDRKSAARPPFAQGRIGVLALNGERIATIGGMAQYSAAEPATVLFSNELGD
                     PLFASREQGNRAVMLFASDLYGTYTSDFFARPEAAGAVKDRLDALFADRPLEASVAET
                     GASVSVSVTSDFLASPRLLLTSAGEMALELPFHRAGPGSWSAEAVPPRAGRWTAAILD
                     RGSSLASFPLPANTGLGGGDAEAAAAVSGWRPRGTRPFRSDALWLGLFFASSLACTIV
                     LRLKR"
     gene            14361..14825
                     /locus_tag="PLG14_00930"
     CDS             14361..14825
                     /locus_tag="PLG14_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOX47719.1"
                     /translation="MDAKLLGAFSASAESTFRDMFGLEAKAAPPRELDSQEDHGWDIT
                     GLVGLAGQAHGVVAFRLTRALAARLLAGSGVAAASEGEKKELEGGLVAEVTNIVAGAA
                     TTALEGLDVEIAPPIVIRGPNHKIGWPAIAPVIALGFSIPEGSFEIDLCMRY"
     gene            15040..16629
                     /locus_tag="PLG14_00935"
     CDS             15040..16629
                     /locus_tag="PLG14_00935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX47720.1"
                     /translation="MNDSNFESLGLSETILKALSGEGYERPTPIQAACIPSLLAGRDL
                     LGCAQTGTGKTAAFALPIIQLLAASGHRPQPKCVRALVVAPTRELAAQIDASFAAYGR
                     GSRLTQACVFGGVGKAPQEKALARGVDVLVATPGRLLDLHGERKLSLAGVEIVVLDEA
                     DRMLDMGFIRDVRKIVALLPKERQTLLFSATMPPSIAALAAGILKDPLRVDISPATPT
                     VELIEQSVAYVDKNDKRAFLTSFIQGGGVGRAIVFTRTKHGADRLVKSLEKSGIAAAA
                     IHANKSQNNRTRTLDGFRAGTLRILVATDLAARGIDVDDITHVFNYELPNEPETYVHR
                     IGRTARAGASGVALALVDAEEKGLLRDIEKLIGRKVPLAAGPAIDEAYKAAAADRALG
                     PKILGTEEHREPRGPRGRPARDDRGGGPRGRQPGGRDSGPRGGGSRFAGGKPSPSQDD
                     ARAAGPRRPSPAKQAPSRSAPSRATASRAAPYNAGRQAPDQDRARSTRAAIAEATGAR
                     LSFSGRGRAEPRGAFRDRKSS"
     gene            complement(16766..18844)
                     /locus_tag="PLG14_00940"
     CDS             complement(16766..18844)
                     /locus_tag="PLG14_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX47721.1"
                     /translation="MVQKPSMRPAALAALLFLAAACLAAGAPQAAAPAAARGEIDLSS
                     LGQGGTARLAGEWGFHWRKFVDPAAPGEPDVFGSLPGHWIDYGMPGLEERGFGSYRLV
                     LRGLDPRLDLGLSVSAFLSSARIYADGKEVWAQGRPGDSAKAEIPEWRNALLRIGPQA
                     DGELELVVHVSNFADRSGGNIMPIAAGDFLSLAAARERTRLAEIFVVGAILAMSLYYL
                     FLHAFRPQDRSSLFFGLLCFVIAVRILCYDEFYLLEILPSLSWAWLFRAGYLTFALAT
                     LLVAAFIRALFPRLVSRWAFLAVLAVTLSYSAVILVLPPLVPSRLLSAYQACAVLVGL
                     YLVTVLARAVAKRERGSVLLSCGFVLFFAAVVFDILVSSGAIVGGFFVTQFGLLAFLF
                     SISLVITKKFAGAFATAEMLTEDLARANRAMKRFVPEEFLSRLGKTSVEQVLLGDHAA
                     QDMTVLFADIGSFTRMSDSMSPEETFQFINEYLARMGPVIRSHGGFVDKYLGDGIMAL
                     FPDSPEKAVRCAVDMHRSLARYNSERAAQGREAITIGIGVHSGRLMLGTIGENERMDG
                     TVISGVVNLASRIEGIAKEYSIGLAVSERILLGLDDPNAYHLRFLGKVPVKGTREPVS
                     VFEVYDGDPEPLREAKDKARGPFEKALAAFYGQEYKDAMAGFKSTLELLPDDEASRHY
                     IRIIRKLDLA"
     gene            19115..20386
                     /locus_tag="PLG14_00945"
     CDS             19115..20386
                     /locus_tag="PLG14_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX47722.1"
                     /translation="MKFAVLAVAVAMYALVIAFSDRKAWFSLGAAALVVALGVVSPSK
                     AAFELVNWNVLLIYIGSLVIADLFIYSRVPAHIADRIVESSPTLGLGIVSILVMTGII
                     SAFVENVATVLVMAPIAIALSKKVGMKPTVFMIGLAVMANLQGTATLVGDPPSMIFAS
                     YAGYSFNDFFFHQGRLSIFFAVQAGMLAGAVYFYFYFKRYGKIQVGLGIEKVLSYLPA
                     ILLVALIAGLAAISFFFEGLALSGLWCMILAAIGLAWYVLVRKEPVKRAWTLVKGLDW
                     ETILFLIGIFVVIGAIAEVGLLEDLARILEGFIGGNMLLGFVVVVGVSVLLSGFIDNV
                     PYIIVMLPVAASLASSLGLPKELYMFGLLTGSCLGGNLTPFGASANVVATGLCKKEGA
                     PVSFGEWLKVAGPFTAITTLASALVIWFVWR"
     gene            20760..21239
                     /locus_tag="PLG14_00950"
     CDS             20760..21239
                     /locus_tag="PLG14_00950"
                     /inference="COORDINATES: protein motif:HMM:NF022654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HOX47723.1"
                     /translation="MSATFSVYFEGPFWVGVLESEPAGSAGPVFAVRHVFGAEPSNAE
                     LLAFMLGGFGALASAVSGAEARASGARAAETGEARAADGSRRAESCRRPNPKRALREA
                     ARDAARPPSTKAQAALAAEREALKAAASSGARARREELAERRFRLRGEKRREKRRGH"
     gene            complement(21133..22209)
                     /locus_tag="PLG14_00955"
     CDS             complement(21133..22209)
                     /locus_tag="PLG14_00955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX47724.1"
                     /translation="MKKSGDPSRRIPAGAPGHPKTGAEGPRGPSRGALRGDSRGGGRE
                     GGKATRDGGRGGRPAQSGEVPPFPSGGRKPATAASRVAFLYEDEDLVAVEKPSGLPVI
                     APEGSRSKNLLDLVTEHIRQRNPKGRGAVVHRIDRDTSGLLVFAKSAKAKRALMEAWD
                     ELVLERRYVALVLGEMGGTEGRLESWLVENRGGEVYVAAPGERGAKRAATNWRLLGSG
                     NGLSLVELELETGRKHQIRVQLAAAGHPVCGDAKYGRAPDPLGRLCLHAAGLVLTQPF
                     TRERLELESPAPPEFGEALRPRGPAGARAPAPRAARAGAKAPDRERAANARGASRGAS
                     PREGGSAAPRAPRAEPGRRRKPRP"
     gene            22344..24158
                     /locus_tag="PLG14_00960"
     CDS             22344..24158
                     /locus_tag="PLG14_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOX47725.1"
                     /translation="MGARRLGIGGKYILLVSLSAAAFIAAALLVSRGLLRDYALSSAD
                     ELAGAVLDLADERFARFFEEMENVAEGLAGVRAVREADPEGMRDLFLATVRARRPYLR
                     GVYLGTPEGAMHEWGEGEGFSDNVPSFPPGYDPRERPWYRTAIARDGFSVSRPYEFAS
                     VGAIGITCVLPVRREDGSLVGVLGLDILLEGLTSVLEGLKIPKGGKALLLSEDGGVIA
                     SQYPGGAGGRRSLGSFEAGGGSSLLGEREGSFVGEYGGTATQFVHRRVEGLGWIVVVG
                     MPLDSVMEPVSSLLDLLGAVGLFLMAFLLAVLVAITGRLVVAPLGHIVSVVNRIEAGD
                     RGARASLRLGGEFGLLCDELNKLADAVEENSRDLEEKARLRGEESSRLQRENTQLRIV
                     EERQRIYRDMHDSIGARLTNVFFCNGVARELARGIAKGGAKELRDMHERIESNCLEAI
                     QDLKEIILGMGEGEGPAPEFPRRLAEGIRKRLGAAGIRLDCRIGSREELAALGSGPRG
                     ELERLFDELVSNVLKHSGASVARLRMKVSDGRLSVSFADDGRGFDPDAAEGSGSGSGL
                     RNIRYRVEGLGGSLRLQAAAGAGAAYSIEIPLKEARHA"
     gene            24151..24813
                     /locus_tag="PLG14_00965"
     CDS             24151..24813
                     /locus_tag="PLG14_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX47726.1"
                     /translation="MRDRKGPEPAVLAVVEDEREVREGLKYLLGLDGRIRIAASFARA
                     EELFAWLGENREPDIVLMDINLPGMDGIEATRLLGRSHPSVLVLILTIFEEEEKILAA
                     IKAGAKGYILKNTRPEALLEQILSVRSEGSPISPIVARRILEEIQRSAPGREPADYEL
                     TPREREVLKDVVDGLTCRELAEKHHIAGSTAKKHILHVYQKLNVSSRAEIVRKALEER
                     LV"
BASE COUNT         3527 a         8709 c         9383 g         3277 t
ORIGIN      
        1 gggcggcggt cgcgaccctc gtcatcattc ccatgcagat agcggtcttc gcggccttcc
       61 cgatcccgac gaccgtggag gcctggttcg cccagttcgc cgccaatccc ctggtcggcc
      121 tcttccacgc cgacttcttc atcatggtcg acaacatcct catcgcggtc atctacctgg
      181 ccttctacca ttcgctgaaa gagacggaca agggttttct ccaggtcgcc gtcgtcctag
      241 gcctcgtcgg catcgcggcc tatatctcct ccaacaggac cttcgagctc ctctcgctgt
      301 ccaggaagta tgccgaggcc gcctccggga gcgagaggct cgtcctcctc ggcgcggggc
      361 aggccatgct cgcaggctgg cagggcacgg ccttcgacgc ctactacgtc ctcaacggcg
      421 tctgcctcct catcgtcgcc tccctcatgt ggaagggcgg cccctacggg aagaccacgg
      481 cggccttcgg cctcgccgct ggcgtcctca tgaccattcc ctcgacggcg gggaccctgg
      541 gcctggtctt ctccctcctc tccctcctcc cctggtatgt cttctcgatc cgcttcggcc
      601 tggtcttcag aaggctcgcg aggcgggagg cctgaccatg gacggcgagt tcggcacgat
      661 cgacgagtac ctcgcgggct tctcgggcag gaccagggag ctcctggagg agctcagggc
      721 ggcgatcagg gcggccgcgc ctgaggcgac ggagaggatc agctaccgca tgccgacctt
      781 cttcctcgag gggaacctcg tccacttcgc cgccttcaag ggacacatcg gcttctaccc
      841 cacgccgagc ggcatcgagg gcttcgaggc cgagctcgcg aagtacgaaa gcgggaaggg
      901 ctcggtgcgg ttccccctgg acgagcccct gcccctcgac ctcgtccgcc ggattgtcct
      961 gcgccgggtc gaggagaatc tcgaaaaggc cgcctttcgg cgcgccaaga agcggaatca
     1021 aggccgatag gaacaacatg cgcgcgctcg ccgtccgtct cgcccgactc ctcctcggac
     1081 tcttcctcta cgccctgggc atcgcggcca cgatgaaggc caacataggc tacgctccct
     1141 gggaggtctt ccacgcgggc gtcggcaagg cgataggcat gagcatcggc aacgtgtcca
     1201 tcctcacggg cctcgccatc gtcctcgtcg ccttcctcct cggcgagaag ctcggcctcg
     1261 ggaccgtcct caacatgctc ctcatcggcg tcttcctgga cgcgatcctc tcgctgggcc
     1321 tcatcccgcg gatggagacc tggatcccgg gcctcgccat gctggccgcc ggcctcttca
     1381 cgatctccct cgcctcgttc ttctacatcg gctcgggctt cggcgccggc ccccgggaca
     1441 gcctcatggt cgccctgcgc cggaagactg gcctccccat cggcctctgc cggggaggca
     1501 tcgagctcgc ggccgtcgtc gtgggctggc tcttaggcgg catggtcggc ctcggcaccg
     1561 tggtctccgc cttcgccatc ggcctctgcg tgcagctgac cttccgcgtc ctgcgcttcg
     1621 acccgacgaa ggtcaggcac gagaccctgg gcgcgacctt cgggatggcg cctaggaggc
     1681 cccgatgagg gtggaggccg tctaccaggt cgaggcgtcg agactctgca agctcctcgc
     1741 gcccgagggc acgaaagtcg agctccgcta gcccctcatt tccccgcgag gatcccctcc
     1801 gcgacaagcc tgagcagctc gggcaggatc ccgaaggcat aggacgcgtg gacgcgctcc
     1861 aggcgctggt ggtactcgcg gccccaggga ccgaggttga gggcggggca ggagaagcca
     1921 gcgggcaggg ggtcgaggcc caaggggcag gccgcggagg cgaggagcct ctccgaggcg
     1981 tcgtcctcgg ggtagaggaa gctcatgtcg gagatgccgg ggaagaaggg gcggctggtc
     2041 gtccggccca ggccgcgggc ctccgcggcc tcaagcgcct tttccacgaa gccgaggagg
     2101 cggccgtccc tgacggagtc gagcctggag cggggatagt agggaggcgc gaggccgacc
     2161 accgcggctg gcccctccag gccggccgcg cgtacggcct cggagacgag tcccatgagg
     2221 gcgcccatcg cgtcgccggc atcggccgcc ttggccgccc tcgcgcgggc ggcctcccag
     2281 gcggcggccc ccgccgcgcg ggcgagctcc tcgagctcgg cccagccgag gaccctgggc
     2341 gtccggtcgg gcagggccga cttccagccc gaggcctccg cgaaggcagc ggcgcgtccc
     2401 cgaagcaggc ccagggcggc ctccatcgcc tcgcccgcga gcctggagac cgcctccagg
     2461 caggacgcgg gaccccggtc gtgggtcagc aggttggccg acacgaagac cgattccggc
     2521 gtcgtcacgt cgtagcggtc gcggagctcc cggtagtaga ggatggtcgg cggggagggc
     2581 ggctcggagc tccgcgcctc cgtggccggc gttccggcgg ccgcgccgcc agggtcgtcc
     2641 gagctcgccg agcccgcgcc gaagaggtcg gcgcactcga tccgcctcgc gtactccgcc
     2701 gcgaggaggg ccgggttgac gccgtcgaaa ggggcgcccg cgtggctcgg ccggccgagg
     2761 aagaggacga aggggtggag cttcccgacg ctgcccagga agaccgcgcg tcccgcctct
     2821 ccctcgccct ggtcgaccgc cgcgtccagg ttgatggcgg cgcgggcggc aaggccccat
     2881 tcggaaagga ggccggggag ggcgcggacg gcggagagca tgccgtggga ggatccctcc
     2941 tcgtcgggca cggcgaggaa gaggagggcg ccgggagcgt catcggccag gcctcccgtt
     3001 ctgtccggtc ccgcgcctgg gcccgcgccg gcgctcgctc cccgcgacgc cgcctcggcc
     3061 tcggcctggg cggcgaagtc ctcgaggagg gcgaggcccg cggccaggcc cgccttcatg
     3121 tccaggagtc ccctccccgg aaggaagtcg cccgaggaga ggtcccggag ggcgcggctc
     3181 tccgtctctc ccagggactc ccctcgcagg ctcgcggcgc gggcctcgtc ctccagggcc
     3241 gcggccaggc ggggcgagag ggcctccgga tcgaaggcgt agggctcgag ggggccgtag
     3301 ttggccgtgg agacgacgtc gtagtgcccg gtcaggatca ccgtcggaat cggcgccctc
     3361 gcgggccgga ccccggcccc gcgcgcgacg gccgccgcgg cgaggacgta gcgctccctc
     3421 ccgtcgtcga gggtttttat gaggcgcaag tgttccggat gggagcggaa atagggccag
     3481 tcggcgagga gatcgcggag gaagcccgcg aaacgggtct cgccctcgct gcctgtgacg
     3541 cttgggaagg aggcgatggc cagggcgagc tctctcgtgc gggtcgggtt catgggacga
     3601 ttgtaccccg ctttcccggc cgggtcatgg ggcggcgcgc ggccgagtcc gcccttaggg
     3661 agtaggtggc gctcccggcg tcggcgcctc gggagaggcg gcgaggttgc gcacccagcg
     3721 ctccttcttg agccaccagc ccgcgaccag ggccttcggg atgtcggaga gcttcgcgac
     3781 gccgaagagg gcgacggggc cccatgcggt gaagcgggcg atcgcgaggg cggcggggat
     3841 gaagacggcg taggtgacgc ccacgtcggc ccagacgccc atgagggtgt ctccccccgc
     3901 ccgggagacg gcgaactggg cgttgaggaa gcaccagagg ggcaggtagg agccgatgac
     3961 gaagaccagg ccgcgggtga cggcccgcgc gtcgccggag aggttgccga agacgagggg
     4021 tatggcgagg gtggccgccg ccgccgccgc cccggcgaag gcgccgaaga cggccgagcc
     4081 cgacatgatc cagccggcct tcagcttgcc ctcctcgagc ctccccgccc ccagggtcgc
     4141 gccgacgatg acgcccgtcg ccgcgtggat ggcggggaag acgaggaaga tgaggttggc
     4201 gatggtccag cccgccgcca tgccggcgac ggtctccgcc ccgccccggc cgttgtagag
     4261 ggcggtgatg atggtctccg agatgaccca ggccgtctcc gagaagagca tcatccccga
     4321 gcgcgagagt atggccccga agagcctccg gtcgacggaa aggagcctcc gcgggctgaa
     4381 ggcgaaggcc ggccgcttcc tcgcgatcat cgcggcgaag gccgcggcct ccaccaggcg
     4441 ggccaggacc gtggcgatcg cggctcccgc gacttccagg cgtggcgcgc ccaggttgcc
     4501 gtagatgagg acccagttga agaaggtgtt gaccagggcc gcgctcgtgg agacgaggag
     4561 gggcaccttc gtgacgcccg tgtcgcggaa ggagctcgac atggctgagg cgaagcctat
     4621 ggggatgaag ctcgcggcga cggcccgcat gtagcgggcg ccctccgcca cgatctgcgc
     4681 gccctcggcg ttgccgccga gcatgaggcg gatcagggcc tcggggaaga ccaggcagac
     4741 gaggaggtgg gcggcggaga tgagggtcgc catgaggagc ttgaagcgga aggcctgttt
     4801 catgccttcc tcgtcgcccg cgccccggtg ctgggacagg tagatgccgc cggccgtgca
     4861 gaccgtgttc acgatgacga ggtagacgaa gttgatctgg ttcgcgacgt tcacgccggc
     4921 catcttcgag tcgcccagcg cggcgaccat gaagttgtcg acgagggaga cgaggccggt
     4981 gacgagggac tggagcatga cggggacgac tatggcgagg gcctcgcggt agaagttgcc
     5041 cgggccgagg aggcccctgt tccggatgat tttgcccatg gtgggccttc ttgtactccg
     5101 actcgggggc cgtgatcaag ggagcgaagg attatgcccg tcctatcccc gggccagcag
     5161 cctcttcccc gtgctctttc tccaggccag gagcttggcc gccgaaagga ggccgaggac
     5221 ggcggaccag aggaggcgcg gcggcggagg cggcggaagg cgcaggacgc cgtccctggc
     5281 gggggcgaag tactcggagg gcggcggcgc gagggggcgg aggctgtcgc ccgagcggag
     5341 gagataggcg cccatggcgg cttcgatcct gccctcgcca ggccgcgtcg agtcgaggaa
     5401 gggcggctcg gcccccgcga ggatggcgcc gtcgtaggcg agggggaggt cctcgttcgc
     5461 gagagcggcg gggcctagga cgaagcgccg ggcccgggcg cggccgccgc ttgcgggcgc
     5521 gggcgcgaag cccggcgcct cgggctcggc gcccgcggcg atgagccttc ctcccgcgag
     5581 ggcgggggaa aggacgaggc ggccgtcctc tggcgcgagg cgggtcgaga cgaggcgagg
     5641 cccgccggcg cgcgccgcgg ctcccgcccc cttcccgccc tcgtcgacga gggcgagctc
     5701 gggcttctcc gaggcggcca ggtaggggaa ggccgcgagg accctggagg agaaggggcc
     5761 ctcggcggag gccgcggttg cgggggacgc cagctcgagg gcgaagtccg cccccgcctc
     5821 gccgtagggg ccggggaggg agacgaggta gaggccgggc gaggagaagc ggaggatcct
     5881 ccccgcgggg ccctcctcga cgccgtagcg ggagggggcg agccgttcga aggggcccga
     5941 gggcgcccgc caggccttca cggagaggtc gccgcggcgg ccggcctcga ctaaggcgac
     6001 gagccaggag cccgaagccc ggtcgtggcg cgcggaggag gggtaggcgg cgaattcgct
     6061 ctcccccgag gcggggtcgg ggaagccgag ctcgacggcc tcgaagccct ccgtccggaa
     6121 ggggagggcg tcggtgagga gcgttatgcg gccgaatccg cggcgcctca gggcggcgag
     6181 ctcgccgtag tcgaccgcga ggaaggcgag ctccccttcg agcctcgccg cggccccgcc
     6241 cccctcgacg ccggagagga ggcggcggag cgggacgagc ctggtcctgg ccgtccgcgg
     6301 gtcgaaggcc agggcgaagg gctccgcctt cctgagcgac tcgtcctcgg cgaggcgccg
     6361 catggcgagg tcgagggggc gggagccagg acgccccgcg agcatggagc gcgagcagtc
     6421 gacgaccagg gcgggccggc cgcggggctc ggccccgtcg cggggcgcgc cgccacccgc
     6481 gagggccagg gccaccgcga gggcgagcgc cgcgtcgagg acgaggtccc agtaggcgcg
     6541 cagggcgcgg aagaggaagg cggcagggcc ccggcgcctc tcctcctcga ggagctcatg
     6601 ggggtagctg agccgccgcg agaggcggag gttcaggaag agcatggccg cgacgggcgc
     6661 gaggaggagg aggaaggtcg cgagggcgct aggcatagag ggcctcgagg aggctccagt
     6721 agagcgtccg cctcggctcg ccgggcctga ggattccgta gtaggcgccg cggcgcgcgg
     6781 cctcgcggaa ggcggcgtcc agctccgcct cgcggcgcgc gtaggcggcc tccgccgcgg
     6841 cgtcccaggg aaggcggagg cgccgccccg tctccgggtc ctcgacgtcg acctccgcgc
     6901 gcccgggcct gaggtcggag aagggcctgt agaggcgcac gaagcagttc cgcgcgaagg
     6961 ggctcgagcg cgggctccag tcccggtcga agaagtctga cacgaggacg aggcgcctgt
     7021 agcgcgaggc ggctcgggcg gaggcgacgg ccgcggagat gtccgtcctc ccctcgggct
     7081 ccaggcccgc gaagaaggag gcgatgggcg ccgtcgactt gccgcccctc tgccgcgaga
     7141 gccgcctcag ggtgcggccc gcgaaggcct cgaggctcgt cggcacgccc agggcgccga
     7201 gcatccaggc gatcgtcgct ccgacgcgct tcgcctccgc ggcgccgtag acgcccaggg
     7261 aggccgagag gtccacgagg acggccgccc cgtcccgctc ctcgtcgtag tactccttga
     7321 cgaagaggcg gtccgtgcgc ccctcgagct tccactcgat gcggcgcggg tcgtccgtgc
     7381 tctggtactc cctgatgttc ttgaggtcga aggaggagcc ggcccgccgg aacatggggt
     7441 cgcggctcgc ctgggcggag aggggagggc ggcggggcgt gcgccgcccg aaggcgcgga
     7501 ccagggcggc ggcctccggg tcgacgaggc cgcgggcggc gtcccgcggc tggccgctcc
     7561 gggcggcctc gccgtcgctc acgccgcgcc cctgtactcg cggcggacgg cggccagcac
     7621 cgcggcgagg atctcctcgg ccgtcacgcc gtcggcctcg gcctcgaagt ccaggatgac
     7681 tcggtggttc gcgaggtcga agaagagggc ctccacgtcc tcgaaggcga ggttcgggcg
     7741 gccggcggcg acggccctgg cgcgggcggc gcggaagagg cccagggcgc agcggggaga
     7801 ggccccgtag cgcaggtact tcctggcggc ctcgacgccg ctcgtcccgg gctgggtctg
     7861 ggccacgagg cgggcgacgt agctcttcac ctcctcgggc acggcgaggg cccgcgaggc
     7921 gcggcggaag gcggcgaggg cggccaacgt ggcctcccgg ccggccggcg cccccagccc
     7981 gggccgctcg gaggcgtcca ggatggagcg cagggcctgg aagtcgggca tcgcgaagaa
     8041 gagccgcatg aggaagcggt ctacctgggc ctcggggagg gggtaggtgc cctcgagctc
     8101 gatggggttc tgggtcgcga tgacgaagaa gggcgagggg agctccctcg tctccccgag
     8161 gaaggtgacg cgcttctcct ccatcgcctc caggaaggcc gattgggtct tgggcgtcgc
     8221 gcggttgatc tcgtcgccca ggaccacgtt ggagaagatg gggccctcgt agaacttgaa
     8281 ggcgcgctcc ccgccggcgg ccgtctggag gacgctcgag ccgatgacgt cgaggggcat
     8341 gaggtcgggc gtgaactgca cgcgcttgaa gccgaggccg aggaactcgg cccaggcctt
     8401 cgcgaggctc gtcttcccca ggccgggcgc gccctcaagg aggacgttcc cctcgcagag
     8461 gaaggcgatg aagagccgct ccacgatgtc gtggcagccc acgacgcgcg actcgaggta
     8521 tgcgtggtag cttttcgcga agtccgccac ggcgcggagg tcggcctcga gctccccggc
     8581 tccgtagtcg cccatcgcga tgtcgttatt ccttcccata gagaaaacct ccgatgacgg
     8641 ccttgtgcag gtataggcgg accagcgccc agagggcggc ggcgttcagg atggagcccg
     8701 cggcgaggag cctcgcggcg cctccgggag cctccgcggc gcggggcagg agcagtcccg
     8761 aggcggccag gttggcggcg aggtgccagg cgtaggcgag ggcgaagccg cccgcgatcg
     8821 cgggcgcggc ccgcgccgcg cttcggccgg ccgggccgcc tcgtcccgag acgaggggga
     8881 acaggccgag ggacagcgcg agggcggaga ccaggtggac gggctcggac cagaggaggc
     8941 ggcccgccgc tccccagccg ggggaggcgg cgaagtaggc caggttctcg atggccgcga
     9001 agaccgcgac cgcggcgatc ccgagggaga gcccgcgggc agcgccgagg cgcaaggcgt
     9061 ccgcgcctcg gcgcctcggc ccgccggccc cgaagcggag gccgaaggcc aggaggaggc
     9121 cgagctttgc tccttcctcg atgagcgcga acagggcgcg gcgggccgct tccggcgagc
     9181 ccggggcaaa aagtagatcc ccgagtccct cgaggacaag catggcgagg tagaggagga
     9241 atcccgaaac ggcggcgagg cccgcctcca ggacggcctg cctgggccgg aacggcctca
     9301 tggcttccct ccgagttcgc gccatcggcg cttcaagagg gctgcgtagg cttcctcgcg
     9361 gggggcgatg ccggcggaga gggcgaagga ctcgaagtag cggcgctcgt agtcccggag
     9421 cctgcccagg gaaagctcgc cggaggcctc cacggcgtcg cgggtgcgct cggcgtagac
     9481 gcgggcgaag cgggcgcggt agtcgaggag ggggctcggc gcgaggctct cgtccgccgc
     9541 ctcgaagccg gcctgggccg gcctgtcccc gcgggcccgc tcggtccctc ggccctccgc
     9601 gggcttggcc atgggggccg cggccttctg tccctcgggg cccgcgcctt cggagccgcc
     9661 cgagccggcc ctcttctcgg cgctcttttg ggccgcgtcg cccagggcca agtcctcctc
     9721 cagggccttc tccttgctct cgtagagggc ccgcgcgcgc tcctcctcgg aggcggcctc
     9781 cctcgcctcg cgcgaggccg cttgggtctc gggcgagcct tccgccttcg cctcctccat
     9841 gccctcccgc ggcgcggaga gggaggccgg atcggcctcg ccgggccggg cgaagccccc
     9901 ctcctcgatg cggctccctc ttccggcggc cggaggcggg gcgtaggaca ggaggggccg
     9961 cccgacggcc acgagggcga ggagctcgaa gagggccagg gccgcgaggg ccgaggccgc
    10021 gaggggcttg agtcccgcga agggcttgag gcggggcggg gtctcgagct cgagctcggc
    10081 ggccctcgcc gcggcgcgga gctcgaggag gtcgcgggcg ggcccgggcg gggcctcgag
    10141 ccaggcctcg aaggtggtgt cccggtccga gacgcgcagg gccgcgcgga ggctctcttc
    10201 cgggatcggc caggcgagga aggcgggaag gagggaggcg aggaggatga ggaggcccat
    10261 ggccgcggcc tccggcgcgc ccgcgaggag gaaggccgcg ttgcccgcgc tccaggcgat
    10321 cgccgaggcg cagagccagg ccgcgaggcg ggcgaggagg gccgagcggc aggagcgcag
    10381 gagcctcccg ccgagggccc gctcggcggc ggggccggcg gggcggctca tggccgcctc
    10441 ctggccgccg cgtagcggct gagggcgagg caggccgctc cgccgcctac cgtggccgcc
    10501 ggggggagcc ggtccagggc ggcggcggcc agggcgaagg cggcggcgtc cgcgaggacg
    10561 agggagaagc cgatggcctt cggggccagg aggtcctcca gggagaggag gaaggcccag
    10621 agggcgaggg aggaaaggag gaagagcgcg aggccggccg gctcggccgc gcccaggcag
    10681 gcggctccgt aggcgaggaa ggggaggcat tcgagggcga ggagggcctc gtcccttccg
    10741 cggcggccca gggcaagggc gccgagccgc gcgtaggtcg cgacggcgaa gaaggccgcg
    10801 cccgggccca gccaggcgga ggcgcccgag taggggttga tggccgccgc cgccccgagg
    10861 agggggagga agaagaggag ccgcttcgcg aacctagtcg cttccatgca gcacccatcc
    10921 cgagagggga gatccggagc gaagggcgta cgcgccctcc cggaagacga cgaggggcgg
    10981 ctccgagaac cggcaatcgt ccttgtcgga ggcgactagg ctcaagggga cggagctcca
    11041 gcccggggtg acgatcctca cgagctcgag gcccctgccg ggatcaccgt agcggagcgc
    11101 ctggaagcct cgcccgcttt ctcgggagag ctccaggctc cccgagtagg ctgagcccct
    11161 cgcggccgcg ggcagggcgg ccgagaaaag gagggcgcgg cgggggagaa gggcgtaggc
    11221 gcccagggcc gccgccagcg aggcggcgca gacgagaccg atgccagcct tgccgcgggc
    11281 cgtcccgccg cgggccgcct cgacgcgggc tgcttcgccg cgggccgcgc ggcggcggaa
    11341 ggccccggcc tcggcgaagg cggccaggag ggcgaaggcg gcgaggaggg ccagggcggc
    11401 gacgggccgg ggcggagaga agttggccgc ggccgcccgg ccggcctcgt cggccgtagc
    11461 ctcggacagc gtcaggcgtt cgagggggac ggacctgggc gtgagcgccg ggggaggcag
    11521 gagggcacgc atgcgctcgc ggcgggagcg ctcgccctga tcggcgccgc ccgtgcgacg
    11581 ctcctccgcc gcgaggtcca gctcgagcct gagcggtccg gcggggctgt ccgcctcgac
    11641 cctgagggcc cttgtctcct cgtccaggtg gaagacggcc tcgacgacgc ccgagtaggc
    11701 tcccgcctgg gcctcgaaga ggaggcgctc ccccccgtcc cagcccaggc cgaagacgcg
    11761 gaaggggccc aggaagccgg cctccgcctc ggcggcgatc cgggagaagc ggtaggggag
    11821 gaaggggccg cccgaggcgc ggaggcccgg cgagccctgg atggcgatgg gcgcggccct
    11881 cggcgccgcg gcggccgcct ggaaggcgcc ttgcggcggt gcggacgacc cgcgcgcgtt
    11941 ggccggttct tgcgcgccgg caagcgccgc cagggcgagg gcggcgagaa ggaaggccgc
    12001 gagccgggag gccgccattc ggggggccgc gagccgggcg gccgcgggag aagccccggt
    12061 cctcatcgct tgagcctcag cacgatcgtg caggccaggc tcgaggcgaa gaagaggccg
    12121 agccagaggg cgtccgagcg gaagggcctt gtcccgcgcg gcctccagcc ggagacggcg
    12181 gccgccgcct cggcgtcccc gccgcccagg ccggtgttgg cgggcagggg gaaggaggcg
    12241 agggagctcc cccggtccag gatggcggct gtccagcgtc cggcccgcgg gggcacggcc
    12301 tcggcgctcc aggaccccgg gcccgcgcgg tggaagggga gctccagggc catctcgccc
    12361 gcggaggtga ggaggaggcg gggagaggcg aggaagtcgc tcgtgacgga gaccgagacc
    12421 gaggctcccg tctcggccac ggaggcctcg aggggcctgt cggcgaagag cgcgtcgagc
    12481 ctgtccttga ccgcccccgc cgcctccggc cgcgcgaaga agtccgaggt gtaggtcccg
    12541 tagaggtcgc tcgcgaaaag catcaccgcg cggttcccct gctcgcggga ggcgaagagg
    12601 gggtcgccga gctcgttgga gaagaggacg gtcgccggct cggccgcgga gtactgggcc
    12661 atgccgccta tcgtggcgat cctctcgccg ttgagggcga ggacgcctat gcggccctgg
    12721 gcgaaggggg gcctggccgc gctcttcctg tcctcgaaga tgagggccgg cacctcgtcg
    12781 ggggaggcga cgggatagta ccttccgccg ccgcggtagg ccaggcgctg gaggagggcc
    12841 tggtcggcgt cgtcgccgac tcccatcgcg gagatcgtcg cgcccagggc ccgcgcgcgg
    12901 tcggccaggg cctggaagtc ggcgggcttc gtgacgccgt cggtgacgag gacgacgtgc
    12961 ttgtccttga ggccctgcag ggaggcgcgg tccaggccct cggagagcgc ggcggccaag
    13021 tcggtgccgc cttttgcgcg caggggctcc aggacgggcg cggcggagac gccgcccgcc
    13081 tccacgaagt cgtagaccca gcggcgctcg tcggagaaga ggagcatgcc gacccggtcg
    13141 ctcggcttca ggttccgcag gagctccagg cccgtcacct tggcgaggga cagcttgtct
    13201 ccgaacatgg agcccgaggt gtcgatgagg atgaggatgg cgaggtctgg caggtccttg
    13261 aggctcttgg gcgcgaggct cgcggggagg agggcctcga gctcggggtc gtcgcccttc
    13321 ttgccgaagt cgggggagtc ggcgacgaag aggatcgagc ccgtgcggcg gcgcgccacg
    13381 tcgaggaggc tctcgcggag gccgccgccg agcccgccca aggggaggcc gtcgacgacg
    13441 acgagctcgt agccgtggag gtccttcgcc gccgcggcct cgggggtcgc ccgctcggcg
    13501 gggtagatcc gctcgatgaa ggaccgcttg tccgcgccct tcgcgctcac gacgaggacc
    13561 cgcggcgcct cggaggactc cgcgcccaag tcgagcctca gggactcctc tcgccctccc
    13621 tcggaggccc tgaccctgag cccctcggcg agcctgtccg ccggggcgga aaggcgcagg
    13681 acgaggtcgg ccgggagctc ggcgccgttg ccccgccaga gggcggttcc gcccgcctcc
    13741 acctcgatcg ccgcgcggct cctcgcctcc ggcgcgaaga ggagctcgaa ggcccgcctc
    13801 tcctcgttcg gcgcgatcct gtgggagagg gcgaggagga ggggcgaagc ctcgtcccgg
    13861 gcgacctcca gggccagggg cagggcggcg agcgaggcgg cgccctggag gcggaggagc
    13921 tcccccgcgg cggggaggcg ccccgcgacg aggcgggccc tgcggagggc ggggaggccc
    13981 gcctcgatgt cggctatcgc gggctccagg gagggccggt cggggaggag ggcggcggcc
    14041 tgctcgtagg ccgaggcggg cgagccgcgg ggtggcaggg ggagaagccg gccggggagg
    14101 agggcgaagg cctcccttgc gccgccccgg gccgcggcca gggcctcggc ccccgctccc
    14161 ttcgccagcg ccacgtagcg gcgctcggag aggaggaagg ccggcaaggg gagggccgcg
    14221 tgggccaggt aggcggccag gaagaggaag ggcagggcgg tcgcggcgca tttggcggcg
    14281 tcccacttgt tccggcgcat tggaactcct attctatatc ctgcaggtgc agtccacaac
    14341 gaccaaaatg ggggaatcag atggatgcga agctcttggg cgccttcagc gcctcggccg
    14401 agtccacctt ccgcgatatg ttcggcctcg aggccaaggc cgcgcctccg cgcgagctcg
    14461 attcgcagga ggaccacggc tgggacatca ccggcctcgt cggcctggcc ggccaggccc
    14521 acggcgtcgt cgccttcagg ctgacccgcg ccctcgccgc ccgcctcctc gcgggctccg
    14581 gcgtcgccgc cgcgagcgag ggggagaaaa aggagctcga gggggggctc gtcgccgagg
    14641 tgaccaacat cgtcgccggc gccgcgacca ccgccctcga gggcctcgac gtcgagatcg
    14701 cccctcccat cgtcatccgc gggccgaacc acaagatcgg ctggccggcg atcgcgcccg
    14761 tcatcgcctt gggcttctcg ataccggagg gctccttcga gatcgacctc tgcatgcgct
    14821 actagcccct gaaggccgca cgggccgccg cctggccgcc ccgtcccgcg cggccggcgc
    14881 ccggcggcgg gcccgcgcgg ctcaggcggg cgagggggcc ttccccctcc cgcgcctttc
    14941 ctctatactc tacccatcaa tgctgatcag cccgagcgcg aattgtccca cggcctttcc
    15001 ccctccacga tcacgcgaac ccacaacccc aggaagaaga tgaacgactc caacttcgaa
    15061 tccctcggcc tctccgagac catcctcaag gccctgagcg gcgaaggcta cgagcgcccg
    15121 accccgatcc aggccgcctg catcccgagc ctcctcgccg gccgcgacct cctgggctgc
    15181 gcccagaccg gcacgggcaa gaccgccgcc ttcgcccttc ccatcatcca gctcctcgcg
    15241 gcctccggcc accgccccca gcccaagtgc gtccgcgccc tcgtcgtcgc acctacccgc
    15301 gagctcgccg cgcagatcga cgccagcttc gccgcctacg gccgcggctc ccgcctcacc
    15361 caggcctgcg tcttcggcgg cgtcggcaag gcgccccagg agaaggccct cgcgaggggc
    15421 gtcgacgtcc tggtcgcgac ccccggccgc ctcctcgacc tccatggcga gcgcaagctg
    15481 agcctcgcgg gcgtggagat tgtcgtcctg gacgaggccg accgcatgct cgacatgggc
    15541 ttcatccgcg acgtgcggaa gatcgtcgcc ctcctgccca aggagcgcca gaccctcctc
    15601 ttctccgcga ccatgccccc ctccatcgcc gccctggcgg ccggcatcct caaggacccg
    15661 ctgcgcgtcg acatctcccc cgcgacgccg accgtcgagc tcatcgagca gagcgtcgcc
    15721 tacgtggaca agaacgacaa gcgcgccttc ctcacctcct tcatccaggg cggcggtgtc
    15781 ggcagggcca tcgtcttcac ccggaccaag cacggcgccg acaggctcgt gaagtccctg
    15841 gagaagtcgg gcatcgcggc cgcggccatc cacgcgaaca agagccagaa caaccgcacg
    15901 cggaccctcg acggcttccg ggcggggaca ctgcgcatcc tcgtcgcgac ggacctggcc
    15961 gcgcgcggca tcgacgtcga cgacatcacc cacgtcttca actacgagct cccgaacgag
    16021 cccgagacct acgtgcaccg catcggcagg accgcgcggg ccggagcctc cggcgtcgcc
    16081 ctggccctcg tcgacgcgga ggagaagggc ctcctccgcg acatcgagaa gctcatcggc
    16141 cgcaaggtgc ccctcgcggc cgggcccgcc atcgacgagg cctacaaggc cgccgccgcc
    16201 gacagggccc tggggcccaa gatcctcggc acggaggagc accgcgagcc ccgcggcccg
    16261 cgcgggcgcc ccgcccgcga cgaccgcggc ggcgggccgc gcggcaggca gccaggcggc
    16321 agggactcgg gcccccgcgg cggcggctcc cgcttcgcgg gcggcaagcc ctccccctcg
    16381 caggacgatg cccgcgccgc gggcccgcgc cggccctccc cggccaagca ggcgccctcc
    16441 aggtccgcgc cctcgcgggc tacggcctcc agggccgcgc cgtacaacgc gggccgccag
    16501 gccccggacc aggaccgcgc ccgctccacc cgcgccgcca tcgcggaggc cacgggcgcc
    16561 cgcctgtcct tctccggaag gggccgcgcc gagccgcgcg gcgccttcag ggaccgcaag
    16621 tcctcctagc cgcggcagcc tccagacgcg aagggcgccc cgccggggcg cccttcgttt
    16681 tttttaggct ggctgtcggc gtcgtcggcg cgggagcgac acgagccgcg gccgagcgtc
    16741 cctgccgcgc ctggctcctg cgcggctagg ccaggtcgag cttcctgatg atcctgatgt
    16801 agtgcctgct cgcctcgtcg tcgggaagga gctccagcgt ggacttgaag ccggccatcg
    16861 cgtccttgta ctcctggcca tagaacgccg caagggcctt ctcgaagggc cctcgagcct
    16921 tgtccttggc ctcgcggagc ggctcggggt cgccgtcgta gacctcgaag acggagacgg
    16981 gctccctggt gcccttgacg ggcaccttgc ccaggaagcg gaggtggtag gcgttggggt
    17041 cgtccaggcc gaggaggatg cgctcgctca cggcgaggcc tatcgagtac tccttggcga
    17101 tgccctcgat cctgctcgcc aggttgacga cgccggagat caccgtcccg tccatgcgct
    17161 cgttctcgcc gatggtgccg agcatgagcc tccccgagtg gacgccgatg ccgatcgtga
    17221 tcgcctcgcg gccctgggcg gcccgctcgc tgttgtagcg ggcgaggctc cggtgcatgt
    17281 ccaccgcgca ccgcacggcc ttctccggcg agtccgggaa gagggccatg atgccgtcgc
    17341 ccaggtactt gtccacgaag ccgccgtggg acctgatgac tgggcccatg cgcgccaggt
    17401 actcgttgat gaactggaag gtctcctcgg ggctcatcga gtcggacatg cgcgtgaagg
    17461 agccgatgtc cgcgaagagg accgtcatgt cctgggcggc gtggtcgccc aggaggacct
    17521 gctccacgct ggtcttcccg agccgggaga ggaactcctc ggggacgaag cgcttcatgg
    17581 cgcggttggc ccgcgccagg tcctccgtga gcatctccgc cgtcgcgaag gcccccgcga
    17641 acttcttggt gatgacgagg gagatcgaga agaggaaggc gaggaggccg aactgggtga
    17701 cgaagaagcc gccgacgatg gcgccgctgg acacgaggat gtcgaagacg acggccgcga
    17761 agaagaggac gaagccgcag gagaggagga cggagccccg ttccctcttg gccacggccc
    17821 gcgcgaggac ggtcacgagg tagaggccga cgaggacggc gcaggcctgg taggcggaga
    17881 ggagcctgga gggcacgagg ggcggcagga ccaggatcac cgcgctgtag gagagggtga
    17941 cggcgagcac cgccaggaag gcccagcgcg agacgagccg ggggaagagg gcccggatga
    18001 aggccgcgac gaggagggtg gccagggcga aggtcaggta gcccgcgcgg aagagccagg
    18061 cccaggagag ggaaggcagg atctcgagga ggtagaactc gtcgtagcag aggatcctga
    18121 ccgcgatgac gaaacagagg aggccgaaga agagggagga gcggtcctgg ggccggaagg
    18181 cgtggaggaa gaggtagtag aggctcatcg cgaggatggc gccgacgacg aagatctccg
    18241 cgagcctcgt gcgctcccgg gccgccgcga gggagaggaa gtcgcccgcg gctatcggca
    18301 tgatgttgcc gcccgagcgg tcggcgaaat tggagacgtg gacgacgagc tccagttccc
    18361 cgtcggcctg ggggccgatg cggaggaggg cgttcctcca ctcggggatc tcggccttgg
    18421 ccgagtcccc cggcctgccc tgcgcccaga cttccttacc gtccgcgtag atgcgcgccg
    18481 aggagaggaa ggccgagacc gagaggccca ggtccagccg gggatcgagc cccctgagga
    18541 cgagcctgta gcttccgaag ccccgctcct cgaggccggg catcccatag tcgatccaat
    18601 gacccggaag ggagccgaag acgtcgggct cgccgggcgc ggcgggatcg acgaacttcc
    18661 gccaatggaa gccccactcg ccggcgagcc tggccgtgcc gccctggccg agggaggaga
    18721 ggtcgatttc gcccctggcc gccgctggag ccgccgcctg gggcgctccg gcggcgaggc
    18781 acgctgctgc gaggaagagg agggccgcga gcgccgcggg ccgcatggaa ggcttttgga
    18841 ccatggcgag gagggtagca tggccgccgg gccggggcaa gcgccgaagc gccccgggcg
    18901 tccctctctt gacgcccggg ggcgcgaaag actatgttcg tccgtgacga agggggagta
    18961 gttgcacctc ggaatcaaca gccggtcgcc gggaggcggc ctggttccgc ggcctcgctc
    19021 tcgaggaaac aagacctttc gcatgcgctc gaggcatcga gcatatgcga aaggtctttt
    19081 gttttaggcc aggcccccaa ggagcatagc cgccatgaaa ttcgccgtac tcgccgtcgc
    19141 cgtcgccatg tacgccctcg tgatcgcctt ctccgaccgg aaggcctggt tcagcctggg
    19201 cgccgcggcc ctggtcgtcg cgctcggggt cgtctccccc tccaaggccg ccttcgagct
    19261 cgtcaactgg aacgtcctcc tcatctacat cggcagcctg gtcatcgccg acctcttcat
    19321 ctactcgcgg gtgcccgccc acatcgcgga ccggatcgtc gaatcctcgc cgaccctggg
    19381 cctgggcatc gtctccatcc tcgtgatgac gggcatcatc tccgccttcg tggagaacgt
    19441 cgcgaccgtc ctcgtcatgg cgccgatagc catcgccctc tccaagaagg tcgggatgaa
    19501 gccgacggtc ttcatgatcg gcctggccgt gatggccaac ctccagggca cggcgaccct
    19561 cgtcggcgac ccgccctcga tgatcttcgc gagctacgcg ggctactcct tcaacgactt
    19621 cttcttccac cagggcaggc tctccatctt cttcgccgtg caggcgggca tgctggccgg
    19681 agccgtctac ttctacttct atttcaagcg gtacgggaag atccaggtcg gcctgggcat
    19741 cgagaaagtc ctctcctacc ttcccgcgat cctgcttgtc gccctcatcg cgggcctcgc
    19801 cgccatctcc ttcttcttcg agggcctcgc cctctccggc ctctggtgca tgatcctcgc
    19861 ggccatcggc ctcgcctggt acgtcctggt gcgcaaggag cccgtgaagc gggcctggac
    19921 gctcgtgaag ggcctcgact gggagacgat cctcttcctc ataggcatct tcgtggtcat
    19981 cggggccatc gccgaggtgg gcctcctcga ggacctggcg cggatcctgg aaggcttcat
    20041 cggcggaaac atgctgctcg gcttcgtcgt cgtcgtgggc gtctcggtgc tgctctccgg
    20101 cttcatcgac aacgtgccct acatcatcgt gatgctcccc gtcgccgcca gcctcgcctc
    20161 gagcctcggc ctcccgaagg agctctacat gttcggcctc ctcacgggct cctgcctggg
    20221 cggcaacctc acgccattcg gcgcttcggc caacgtcgtc gcgaccggcc tgtgcaagaa
    20281 ggaaggcgct cccgtgagct tcggcgagtg gctcaaggtg gccggcccct tcaccgccat
    20341 caccaccctg gcctcggctc tcgtgatctg gttcgtctgg cgctaggcga ggcggccggc
    20401 ggcgccggag tccggccgcg ctggcgcctg gcggcgcagg agtccggccg cgctggcgcc
    20461 gccggcgctg gagtccggcc gcgctggcgc cgccggcgcc cggcggcgcg cccgtgcggc
    20521 ctctgcccgg cggcgcgccc gtacggcctc tgcccggcgg cgcccggact tggcaaagcc
    20581 tgcccgcagg gagtagcgtg aggcagtttc ctcgtaacgc aggcaaggga aaaaacacgc
    20641 gccggtaggt agtccggcgg ggtccttggc gggcgcctgg cgcgcccccg ggactcgagt
    20701 agccagcgcc gtcggcgccg tccgttcctc gccgggggta gtccgcccgg aggatcgcga
    20761 tgagcgcgac cttttcagtc tatttcgaag gcccgttctg ggtcggagtc ctcgagtcgg
    20821 agcccgccgg gagcgcgggc ccggtcttcg ccgtccgcca cgtcttcggc gccgagccct
    20881 cgaacgccga gctcctcgcc ttcatgctcg gaggcttcgg cgcgctcgcc tccgcggtct
    20941 cgggcgcgga agcccgcgcc tccggggccc gcgccgcgga gaccggcgag gcccgcgcgg
    21001 ccgacgggag ccggcgggct gagtcctgcc ggcggcccaa cccgaagcgg gcgctccgcg
    21061 aggccgcgag ggatgccgcc cgacccccct cgacgaaggc ccaggcggcc ctggctgcgg
    21121 agcgcgaggc cctcaaggcc gcggcttcct ccggcgcccg ggctcggcgc gaggagctcg
    21181 cggagcggcg cttccgcctt cgcggggaga agcgccgcga gaagcgccgc gggcattagc
    21241 ggccctctcc cggtccggag ccttcgcgcc ggccctcgcc gcgcgcgggg cgggagccct
    21301 cgcgccggcc gggccgcgag ggcgcagcgc ctccccgaac tcgggcggcg ccgggctctc
    21361 gagctcgagc cgctcgcggg tgaagggctg ggtgaggacc aggccggccg cgtgcaggca
    21421 tagcctgccc agggggtcgg gggcccggcc gtacttggcg tcgccgcaga cgggatggcc
    21481 cgcggccgcg agctggacgc ggatctggtg cttccgcccc gtctcgagct cgagctcgac
    21541 cagggagagg ccgttgccgc tcccgaggag gcgccagttc gtcgccgcgc gcttggcccc
    21601 ccgctcgccc ggggcggcga cgtagacctc cccgccccgg ttctcgacga gccaggactc
    21661 cagcctgcct tcggttccgc ccatctcgcc caggacaagg gcaacgtagc gccgctcaag
    21721 gacgagctcg tcccaggcct ccatgagggc gcgcttggcc ttcgcgctct tcgcgaacac
    21781 gaggaggccc gaggtgtcgc ggtcgatgcg gtgcacgacg gcgccccggc ccttggggtt
    21841 cctctgcctg atgtgctcgg tcacgaggtc gaggaggttc ttggaccgcg agccctcggg
    21901 cgcgatgact ggcaggcccg agggcttctc cacggcgacg aggtcttcgt cctcgtagag
    21961 gaaggcgaca cgggaggcgg cggtggcggg cttgcggccc ccggagggga agggcgggac
    22021 ctcgccgctt tgagcgggac gccctccgcg tcccccgtcg cgggtcgcct tgccgccttc
    22081 gcgtccgccg cctcgggagt cgccgcggag cgcgccccgc gaagggccgc gcggcccctc
    22141 ggcgccggtc ttgggatggc cgggcgcgcc ggccggtatt ctcctggaag gatcgcccga
    22201 cttcttcatg ccgacgagta tagggcctgg gccctgtccg ggtcgagcgg gcaggggagc
    22261 ctcatgcggg gaaattccgc cttgcccgcc gggcgggatg cctttacaat ccaagttccc
    22321 gcggaaggag aggcgaggga accatgggcg caaggcggct cgggatcggc ggaaagtaca
    22381 tcctcctcgt ctcgctctcg gccgcggcct tcatcgcggc cgccctcctg gtcagcaggg
    22441 gcctcctccg ggactacgcg ctctcgtccg ccgacgagct cgccggcgcg gtcctggacc
    22501 tggccgacga gcgcttcgcc cgcttcttcg aggagatgga gaacgtcgcg gagggcctgg
    22561 ccggcgtccg ggcggtccgc gaggccgatc ccgaggggat gcgcgacctc ttcctggcga
    22621 ccgtccgcgc gcgccggccc tacctccgcg gcgtctacct gggcaccccc gagggagcga
    22681 tgcacgaatg gggcgagggg gagggctttt cggacaacgt gcccagcttc cctcccggct
    22741 acgacccgcg ggagcggccc tggtaccgga cggcgatcgc gcgggacggc ttctccgtct
    22801 cgcggcccta cgagttcgcg agcgtcggcg ccataggcat cacctgcgtc ctcccggtcc
    22861 gccgcgagga cggctccctc gtcggcgtcc tcggcctcga catcctcctc gaggggctca
    22921 cgagcgtcct cgagggcctg aagatcccca agggcggcaa ggccctcctc ctctcggagg
    22981 acggcggcgt catcgcgagc cagtaccccg gcggggcggg cggaaggcgc tcgctcggga
    23041 gtttcgaggc gggcgggggc tcctccctgc ttggggagcg agagggctcc ttcgtcggcg
    23101 agtacggcgg gacggcgacg cagttcgtcc accgccgcgt ggaggggctc ggctggatcg
    23161 tcgtggtcgg catgcccctc gactccgtca tggagccggt gtcctccctc ctcgacctcc
    23221 tgggcgccgt gggcctcttc ctcatggcct tcctgctggc ggtcctggtc gcgatcaccg
    23281 gccgcctcgt cgtggcgccc ctgggccaca tcgtctccgt ggtgaaccgg atcgaggcgg
    23341 gcgaccgggg cgcccgcgcg tcgctccggc tgggcggcga gttcggcctc ctctgcgacg
    23401 agctcaacaa gctcgccgac gcggtggagg agaactcgcg cgacctcgag gagaaggcta
    23461 ggctccgcgg cgaggagagc tcccggctcc agcgggagaa cacccagctc cggatcgtgg
    23521 aggagcggca gcgcatctac cgcgacatgc acgactcgat cggcgcgagg ctcaccaacg
    23581 tcttcttctg caacggcgtg gcccgcgagc tcgcgcgcgg gatcgcgaag ggcggggcca
    23641 aggagctccg cgacatgcac gagcggatcg agtccaactg cctggaggcg atccaggacc
    23701 tgaaggagat catcctgggc atgggggagg gcgaggggcc ggcgccggaa ttcccccggc
    23761 gcctggcgga gggcatccgc aagcggctcg gcgcggcggg catcaggctg gactgccgca
    23821 tcgggagccg cgaggagctc gcggccctgg gctcgggccc gaggggcgag ctcgagcggc
    23881 tcttcgacga gctcgtgagc aacgtcctca agcactcggg cgcctccgtc gcgcggctca
    23941 ggatgaaggt ctccgacggg aggctctcgg tctccttcgc ggacgacggg cggggcttcg
    24001 atcccgacgc cgccgagggt tcgggctcgg gctcgggcct caggaacatc cgctaccggg
    24061 tggagggcct cggcggctcc ctgcgtctcc aggctgccgc gggcgcgggc gcggcctact
    24121 ccatcgagat accgctcaag gaggcgcgtc atgcgtgaca ggaagggtcc ggagccggcc
    24181 gtcctcgccg tcgtcgagga cgagcgcgag gtccgcgagg ggctcaagta cctcctcggc
    24241 ctggacggca ggatcaggat agccgcctcc ttcgcccgcg cggaggagct gttcgcctgg
    24301 ctcggggaga accgcgagcc cgacatcgtc ctcatggaca tcaacctgcc gggcatggac
    24361 ggcatcgagg ccacgaggct cctcgggcgg agccatccct ccgtcctcgt cctcatcctc
    24421 accatcttcg aggaggagga gaagatccta gccgccatca aggcgggggc gaagggctac
    24481 atcctcaaga acacccggcc ggaggccctc ctcgagcaga tcctgagcgt caggtccgag
    24541 ggctcgccca tcagcccgat cgtcgcgcgc cgcatcctgg aggagatcca gaggagcgcc
    24601 cccggccgcg agccggccga ctacgagctc acgccccggg agcgcgaggt cctcaaggac
    24661 gtcgtggacg gcctcacctg ccgcgagctc gcggagaagc accacatcgc gggctccacg
    24721 gccaagaagc acatcctcca cgtctaccag aaactcaacg tctcctcccg ggccgagatc
    24781 gtgaggaagg ccctggagga gcgcctggtc taggcgcctc gccgtcgcct cctcggcgcc
    24841 agccgtcgtc tccgagccct ccgggcggcg cggccaaaat actcccttcg gagcat
//
LOCUS       DAOOSJ010000008        24139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1456_length_24139_cov_5.776200, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000008 DAOOSJ010000000
VERSION     DAOOSJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 24139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24139
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_24139_cov_5.776200"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLG14_00970"
     CDS             complement(<1..259)
                     /locus_tag="PLG14_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47727.1"
                     /translation="MAKRNVIIIGAAGRDFHNFNTYFRNNENYNVVAFTAAQIPDIAG
                     RKYPAELAGKLYPAGIPIHLEADLPKLIKELKVDECVFAYSD"
     gene            713..1957
                     /locus_tag="PLG14_00975"
     CDS             713..1957
                     /locus_tag="PLG14_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOX47728.1"
                     /translation="MPGLNVTSEIGALKRVVLHSPGPEVEAMTPKEAAEDLYNDIIPL
                     SAVRAEHDQLKSFLSRVAKVHELKDLLAESLASPGSRFEFLTAMARLCPIEPIIDELT
                     GLHPVDLADLVIEGLPAKPSSLAGILSPRSFVMRPLPNAYFMRDSAAVFRDWAVSCAT
                     AFDVRLIESIVTRFVFTRHPEFRAKGLLVDGPRARNRFVSIEGGDILVVGPRVLAIGI
                     SERTSPDAVELLAKNAGRAFEEPVTILAVVVPKERATIHLDMVFTMVDRDAALVYAPV
                     VTGPNRAEVVRIDVDPHGRASYSETDGLLPALKALGWDLEPILCGDGHHLYQDREQWW
                     SGANSFAFAPGKIVMYSCNVRTLDALSRRGFAVKRAVDFEDGREDPFAPGRLAVGFDG
                     IELARGGGGARCMTLPVEREEL"
     assembly_gap    2084..2183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2527..3309)
                     /locus_tag="PLG14_00980"
     CDS             complement(2527..3309)
                     /locus_tag="PLG14_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX47729.1"
                     /translation="MIDNPVLSVLRGRASVRKYRKEQPSEEVIEAVLSAGRQAPFASQ
                     LYSVLLTRKKKAPFGAPLWFVVCVDAHKLERFMALRGWKVATNDLTLLLLGMQDAAYA
                     AENMVIAAESLGMGSCFLGEGSINAKRIGAIAKQYKLPKRVLPMVELVMGYPEERLAP
                     RPRYPRPFVLFEDRYPELGEAEVRDAMRAMDEGYLAEGYYAKLGNKIELEDGREDRFT
                     FADYSWTEHISRKWGQWEPFTDELLAAFRERGFDLGPNAPKA"
     gene            3456..3650
                     /locus_tag="PLG14_00985"
     CDS             3456..3650
                     /locus_tag="PLG14_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47730.1"
                     /translation="MLYSLSTLSKEQLESLHALETKLGKPVLAFSAYRDEPAGLSSAE
                     LEALRESESRLGLTLVAVKA"
     gene            complement(4176..7922)
                     /locus_tag="PLG14_00990"
     CDS             complement(4176..7922)
                     /locus_tag="PLG14_00990"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOX47731.1"
                     /translation="MKFERRFTKKGKGPYEGIRWEERRSEIRNPDGRAVFQMEGFVVP
                     STWSQIAADIIAQKYFRKAGVPADKAEAFKAFLPADQRGLAASGGAAAGGAKKPGAET
                     DARQVFHRLAYTWTLWGKEAGYFDSEEDSDAFYEELLYMLAHQMAAPNSPQWFNTGLH
                     AVYGIEGPAQGHYYVDPKTGEVVKSESAYARPQPHACFILDIEDDLVNEGGIMDLVTR
                     EARLFKYGSGTGSNFSKIRGANEPLSGGGVSSGLLSFLKIADRSASAIKSGGTTRRAA
                     KMVILDADHPDIETYVNWKAEEEYKVACLAAGSAVLKRYAADLLSAAKAGGLAGDDRF
                     SFEKNPALRKAAREALRGGLPAPFLHQTLSRFRQGDESLDLPAYDTAWEGEAYNTVAG
                     QSSNNSVRLDNRFMKAVEEDAEWDLVGRISGKPMRRIRARALWDKIAQAAWKCADPGL
                     QFHGTVNEWHTCPADGEIRGSNPCSEYMFLDDTACNLASLNLLAFYDAKAKKFDDEGY
                     RHAIRLWTTVLEISVVMAQFPSKEIARKSYDYRTLGLGYANLGSLLMVMGLAYDSDEG
                     RAVAAALAAVLTGEAYAQSARMAAAFGPFARYAANAKDMLRVVRNHRRAAVAAPEAEY
                     EGLTVRPFALDPAKCPQNLARAARESWDEALAIGEKSGYRNAQVTAIAPTGTIGLLMD
                     CDTTGVEPDYALVKFKKLAGGGYFKIINTSVPPALRALGYSEAAIKEIETYALGHETL
                     KGASTPDGKGGRRPLALSYESLAAKGLPLAALDKAEAALKTAISLESCFAPYVLGKEA
                     YEAVKVPEATYGLPGFSLLAHLGFSGEEIEEAELFACGTMGVEGAPGLKRDHESVFDT
                     ATPSGKRGTRAIEWKAHISMMAAVQPFISGAISKTINMPNSATYEDVKGAYSLAWRSM
                     LKAIALYRDGSKLSQPLSALAPGADIIADSIVALQSGGLEEAEASAEAAASAVTASQA
                     ASALRVQATGLAAPAPELPGLPAAPRGVRRNLPNRRAGYTQKAKIGGHSMFVRTGEYD
                     DGSLGEIFLDMHKEGAAFRSILNSFAIAVSLGLQYGVPLEEYVDAFTFTRFEPNGMVQ
                     GHDYIKMSTSVLDFIFRDLAISYLDRRDLGQIKPEDLEATATQSKRGNGGERVPASRA
                     FEVGRARGVQAPPKASPAPLSAAPAALNQPAAASPALAAAASAPQAGAGQAAAGLSKP
                     KESARNARLKGYEGDPCPVCGHLTLVRNGTCLKCETCGSTTGCS"
     gene            8145..9794
                     /locus_tag="PLG14_00995"
     CDS             8145..9794
                     /locus_tag="PLG14_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HOX47732.1"
                     /translation="MRTAAVVYAGSLSESARKPLAGGGSAYARVLDFVRELPGFEGAL
                     VLSTPGSGDLPAGQASGRLRLETREAFSLSSILEAAEGYAASFQEAGGGEGRGGLDAL
                     VFVRADEPLLDRSLAARMLENFRRYRADYSFADGYPLGLASEILHPRALPALRLLAER
                     HPLEPERGWLFSLIQKDINSFDIETEISPKDLRDRRLCLACDSRRNLLLVERLMAAGV
                     SDAASALEVIPARPELERSLPAFYQVQVEGGCLQSCALCPYPLFGGDILKRRDHMTRD
                     RFAALMGAIEAFSGDAVVDLSLWGEPSLHPDIEGLVEEVLSRPGLSLIVETSGLGWSR
                     GTLESLASRWAGPSGAGGAAGQGGAEGQSGPGGASPRLNWVVSLDAPKPELYARLRGP
                     GYEEATAAAELLLELFPKGAHVQTVRAVDNEPALEDFWRGWKKRTENVIVQKYSRFAG
                     FLPERKVTDLSPLVRRPCWHLKRDVSVLLDGTVPLCRDCVRNEIVLGKLFDESGPNDP
                     GSVLARLEAAWAAGEAYHRLHIEGRHPEPCAGCDEYYTYNA"
     gene            9820..10734
                     /locus_tag="PLG14_01000"
     CDS             9820..10734
                     /locus_tag="PLG14_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47733.1"
                     /translation="MGGPKGRSQGEGLPVSVLLLDRGSRLYRSELFRELDRLGFESVV
                     SVEEGSEAVDVEALSQRFPKVRFLLLGGKASPGEKINLGMRESSSPYVFALWSDMRLV
                     ASGLSGRFFERLAEQNLLCLAPFLQAKRGETLPTAAAPALERQNLRILPLVPARDGAL
                     SLYPFDYAGIYSREKFVLSGGYDGGIANPYWQKLDFGFRSWLWGEEIRLSQALRVAYE
                     EEPPTEDSTPDEDYKWFWLKNLAPVFRGDSAAIPPSRYWAYLRRRGGRPFAAAREFRA
                     AADWVRTNRYRFRSGASSVVDLWEEAKP"
     gene            10731..11441
                     /locus_tag="PLG14_01005"
     CDS             10731..11441
                     /locus_tag="PLG14_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOX47734.1"
                     /translation="MSRPSRLGLVVQARLGSTRLARKALLPLGGLTMADQVLRRLILV
                     PAEVYVLATDEASAPELGPIAARNGFELLVGPAEDVLARYCLAVRRFGLGRVLRATGD
                     NPLVAHELAATLVSRAEAAEAAGAPPADYTSYSGLPLGMGVELVRAEALLRAEAEARE
                     LPEREHVCPYLYGHPELFRLDRPEAPEPWRFPEARVTVDTAADYEAVLRLYGALYSGS
                     PVPTELVYAYLRGQGRAA"
     gene            11438..13309
                     /locus_tag="PLG14_01010"
     CDS             11438..13309
                     /locus_tag="PLG14_01010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX47735.1"
                     /translation="MSRRVLFVPSVAKGNGSGHLVRCLSLARELGPGAAVFAPEAKAA
                     GSWSAAELSLSYSREFLGVPLVSEQAASQAWDLVVLDRRATSAEELAYWERLGPVAAL
                     DEGGPAREAASYLVDILPRAKPPRRGKGRFEAPNAASIAYLDLPRKRREPPRAFSRVL
                     LSFGGEDPAGLGPALARSLVSEGFYPPESLTLVSGALRGPSDRAEGLSGVTVLGPIQD
                     LKEHLAQYDLVFTQFGLTAFEAAFAGCGVVLLNPSAYHRELSVRAGFPEIGVGKPRVA
                     ALRRLLSDSEGTLARTRRIAPESRASLAALLAGLAPSGPRSCPRCGSGERRALFRNAA
                     KSYFRCSSCGTVYLQRFEPGRENPYKESYFFDEYRRQYGKSYLEDWPALTRLADSRLD
                     LIEGLAEKSLGRRSGLSLLDVGCAYGPFLKAARERGQEPYGLDMAEDAARYVRKELGI
                     PAAAGDFLDPAVAAAFGGPFDVVSLWYVIEHFADLDLALRNAAALLRPGGVLALSTPS
                     GEGVSAKFDREGFFERSPDDHFTVWEPSRAGGILKAYGFRVELRRITGHHPERFPFLA
                     GLPAGWPRRAALWLGLGVSRLFGLGDTFEIYAVREPAGPSGPSLPSRKRRERASPRS"
     gene            13326..14963
                     /locus_tag="PLG14_01015"
     CDS             13326..14963
                     /locus_tag="PLG14_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOX47736.1"
                     /translation="MTVFILGAGVMQIPAIRIAKELGWRVAAADGNPAAPGAALADLF
                     FPVDLKDREGLLEAARGLARDGGLEAVFTAGTDFSASVAYVAGSLGLPGHSLEAATKA
                     SDKPLMRAALAAAGVPSPRFAEFRSARDLPAALKAASLAFPLVVKPADSMGARGCRLV
                     SSLAELEAALADALPHSRSGRAIVEEYLDGPEFSIDALVQDGKVAVRGLADRHVFFAP
                     YFVELGHTMPSAFQKEVLDEVLRVFEAGVKALGLTRGAAKGDIKYCPGRGRAYVGEIA
                     ARLSGGYMSGWTYPYASGIEVTRGALELAAGLGPSDGAADRGWVSAERAFISIPGRAA
                     SVLGLREAEAAPYVKNLFLRVAPGEACVFPSNNVEKCGNVISQAPSREAAVSAAEGAV
                     RGILVRLRPAEAATEAFLRGEGRVANPDGGAWPPEAFSPGPRLAAFLAALPERSDEAD
                     GGLKRAGAEGLAASVRLLVAPLPEELRTEAATMSPDWSGRGFLESAELALRLGGARLA
                     PEGAPALAGRFWRALARGGAQAALYVLDTEARGGEGA"
     gene            14960..16108
                     /locus_tag="PLG14_01020"
     CDS             14960..16108
                     /locus_tag="PLG14_01020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOX47737.1"
                     /translation="MRREPRAALAACAFLLILVGRAPAAEGSAVPAAKPGSAPAASSP
                     VPAPAPAAAPVKAKAELSAPAAAERLGAKLRWDPLTGTGSFERGGNLLSFALGSPYAL
                     FRREELIGLDAPRDGGNGPLFTEAAFSAIEGGFSRAEAAKKSHFSIAAILIDPGHGGK
                     DSGAVGEHAVGGKKLRVVEKDLALDLSLRVYRALKAAYPDRRILITRSGDSYPSLEDR
                     VSMANEVELEDNEAIIYVSVHANASFNKNAKGFEVWYLNPEYRRTLVAQDKAKDLGED
                     IAPILNAMLEEEFTTESILLARSIMDGLSSGIGRESPNRGVRAEEWFVVRNAKMPSVL
                     VEAGFVTNPEEAALLADDDYLRRLSEGIYTGIVDFVSYFENMKGSQAR"
     gene            16105..16680
                     /locus_tag="PLG14_01025"
     CDS             16105..16680
                     /locus_tag="PLG14_01025"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOX47738.1"
                     /translation="MKKFLQKAGPFLRRVLDWLAPADHLALAALGLAFLVSLGAWSLG
                     DRLEDATLYFPSYRTGSLRGETRALPRARGAEARAELVASELLLGPGAPGLLPAFPAG
                     ARVESSVFRKGRLYLDLSEDAALSDRADLERGLSALRRSLKAALPLAKGVVVTIGGRL
                     PYADYPEPEPPADGPETARAGAGAEKKGKKN"
     gene            16759..17841
                     /locus_tag="PLG14_01030"
     CDS             16759..17841
                     /locus_tag="PLG14_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOX47739.1"
                     /translation="MRRQIIAVALALLFVGAAALSADEAVLMDFSLLKADIIADPVNQ
                     GKFLENRNTMMDFSGIAGAGYTEEQKKQMRVSLAIPNWDVVLASSSRNNMNQTLSMTA
                     EATVAQDAKQFGGKTVLGMRVRFPTEPFNSWAAVKPPYEIPAFEPKADIDDQGNITPK
                     KDAQGSDPVNARMSRFEGNYDANTKITTALGIVKNVGSLKTVAVTVKGLNFPHGFSVI
                     LKDSDGNERNMFMGYLNFDGWKELRWDNPQYVTDVRNRELRIYPLYPKTVPFIKLDGF
                     LVTRDAANEGGDFVTYVKDVKILYDKAVLEPVQDIDDEAIWGIVGKKESERKILESRR
                     FGSQQVMRYIERQKQETKSDFTPATK"
     gene            17941..19206
                     /locus_tag="PLG14_01035"
     CDS             17941..19206
                     /locus_tag="PLG14_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX47740.1"
                     /translation="MSGEATDPIPDRGLLESDYERRKSSYQAAIEDMERLVRDRLEAE
                     GLRPTIKGRIKSFDSLYKKRIRLLRQAMASGAERPLPINDIIGLRVVCPFLGDLAEAE
                     RILSRDFRVLETERKGADRSFREFGYESIHLLVALPPELGAARSGLDVDACEIQLRTI
                     LQEAWAEVEHELVYKAEFTPFDEPMKRKLAALNANLSLSDIIFQEIREYQRRLSEELA
                     RRRSAFYSKIEEAIDQPFGSAMADGRPSRPAACQDRSVDDRSAAASLDDLLLDALYAH
                     NRGDYAEAIAIYGRILENSPPKEAAAVVTKHRGMAYFSQSRYEEAVQDFSRTLELDGD
                     CYKAAYFRGVVRSVQGQYGKAVEDFDLALRINPYQFFAIYRRAQAYWHLGDYPKVVAD
                     CETANRILPGRPEVMELKSLAMEKLDLLA"
     gene            19248..19319
                     /locus_tag="PLG14_01040"
     tRNA            19248..19319
                     /locus_tag="PLG14_01040"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19281..19283,aa:Glu,seq:ttc)
     gene            19640..21040
                     /locus_tag="PLG14_01045"
     CDS             19640..21040
                     /locus_tag="PLG14_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOX47741.1"
                     /translation="MNEGDRIGNYRLEAKLGSGETGSTWLVRSVATDPLTGHGEAVLK
                     ILDLGEARGWSGYDLFLREIEALKNLSHPGIPRFLDCFEDFSEGGARFALAMERMPGQ
                     ALESLAAAGRRFSEEEVAAILAALADILDYLSSLRPPVVHRDINPRNVLLGPEGKVSL
                     VDFSGAQEAVLRGSRPGATLIGTAGYIPLEQVSGKATVRSDRYGAAATALFLLARANP
                     ADLPLKDLKPDLSSLPSLGRGLAAILSSWLEPDEARRDLEPALASRILRGKTPVPEPV
                     VEAPRKAASRGGKGGAASLPLNSRVRIEEGPDRLRITIPPLGLGTAASLGAGGFVLFW
                     LGFVGFWTFSAAAMGAPLVFILFSLPFWAVGIALGRAVALAAFARTELVLDRKSGLAL
                     SRRLLGKGRGRHCPAADLGECVIVNSPVRIQGRPQRQLAIEAGAESLRIGTGLSEREL
                     DAIRAKIEAWRGEFMR"
     gene            21191..21661
                     /locus_tag="PLG14_01050"
     CDS             21191..21661
                     /locus_tag="PLG14_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47742.1"
                     /translation="MKKKVLLLALVVALVAGANAFAYRAAVGGEFAMKIGQGLPNSAL
                     LSFRLPSFPAVFGLGLTVGESTSSLALLADWWLYQGTLVDSVGLYVGPGAFLGVSADA
                     FTLGLRVPVGINIYPIKPLELFVEFAPAVTFIAPSGVQIPAWGVQAGFGFRFWF"
     assembly_gap    21663..21762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21763..22070)
                     /locus_tag="PLG14_01055"
     CDS             complement(<21763..22070)
                     /locus_tag="PLG14_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOX47743.1"
                     /translation="MSDGKLFDKSVEEWQAEREILNEKVLSLAGLEIKRFFSLDGQTY
                     RDGALSAKTKELLGLVASLVLRCDDCILYHTIRCKEEGVTSGEFQEAAAVGLVVGGSI
                     V"
     gene            22192..22548
                     /gene="trxA"
                     /locus_tag="PLG14_01060"
     CDS             22192..22548
                     /gene="trxA"
                     /locus_tag="PLG14_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOX47744.1"
                     /translation="MSEKLTKDSFKAKIFDWEKNKEWNYAGDLPAIIDFYAEWCGPCK
                     MVAPVLEELAAKYEGKLAVYKVDTDAEPELSAMFQIQSVPTLFFVPKVGEPSFALGAL
                     PKKDLEKAISEVLKVS"
     gene            complement(22630..23820)
                     /gene="thiI"
                     /locus_tag="PLG14_01065"
     CDS             complement(22630..23820)
                     /gene="thiI"
                     /locus_tag="PLG14_01065"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOX47745.1"
                     /translation="MEILYLVKIGEILLKLGNRKEFEARLKADLKRRLEGSVRRIEYF
                     PGRIFVEAEPGKEAFVEAVLARTPGINGFARARRVEKKPEAVFAAAVEAAREAAASGK
                     ARFKAESRRSDKSFPLDSYELSRDIGARVLEALPGTKVDLHAPEFTVFTEIRERAYVY
                     ADPEKGVRGLPSGSQGRGLLLLSGGIDSPVAGYLMARRGLGLDAVYFHAYPYTSKEAE
                     DKVIALAKVVAGYTGGLTLWTVPFTETQMRIKQRAREDLTTLMMRAAMMELSEKLAFR
                     VGANSLVTGESLGQVASQTAENLRFTNSRVSLPVFRPLVGMDKEDTIELARKIGSFEI
                     SILPYEDCCVLFSPKHPLLRAEVERESKAYEELELGPLLEQALKEATKLAVPFGVPVA
                     APSA"
     gene            complement(23833..>24139)
                     /locus_tag="PLG14_01070"
     CDS             complement(23833..>24139)
                     /locus_tag="PLG14_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOX47746.1"
                     /translation="LPLGRTAGDGRYSPFILSIAFPGLSGEVFARALADEGVAVSTGA
                     ACSANARKKGRRILEAMGLEAGLAFSAIRVSTGPATAPEEIDRFLETADALYRRLKT"
BASE COUNT         3522 a         8452 c         8183 g         3782 t
ORIGIN      
        1 cgtcggagta ggcgaagacg cactcgtcga ccttgagctc cttgatgagc ttgggaaggt
       61 cggcctcgag gtggatgggg atgccggcgg ggtagagctt cccggcgagc tcggcggggt
      121 acttgcggcc cgcgatgtcg gggatctgcg cggcggtgaa ggccacgacg ttgtagttct
      181 cgttgttccg gaaataggtg ttgaagttgt ggaagtcgcg gcccgcggct ccgatgatga
      241 tgacgttgcg tttcgccata gagtaccttc cttttgcgta ggcggggtta ggcggaaaag
      301 cccttgtcgg gcctcaccgt cggcgcagta taaacccagg cggaaaactg tcaagcgagc
      361 aattccgcgc cctgaaagga gctaggcggc gccgacggaa tgtatatgcg cttgtcggcg
      421 cggcgaggcg gaaataaatg cgcgtccgga ggcccgggcc tcatagatat gcagaatggc
      481 gccccgacgg gccttcggaa aaaaaagaga aaaaatgcgc gaggggctgg ccgccgcttg
      541 cccgcccctc cgcctcgcgg ccgcgggacg ccgccgggag ctccgaaggc cttcgccggg
      601 cgcgcaagcg gcgccgccgc gccgccgctt ggcctccggg ccgccctcgg gcctcccgat
      661 tgaaggaagg tctccaaatc gttaggatgg gctttctgca aaggagatcg agatgcccgg
      721 actcaacgtt acgagcgaga tcggcgccct gaagcgcgtg gtcctccaca gccccggccc
      781 cgaggtcgag gcgatgaccc cgaaggaagc cgccgaggac ctctacaacg acatcatccc
      841 cctctcagcg gtccgggccg agcacgacca gctcaagtcc ttcctctccc gcgtcgcgaa
      901 ggtgcacgag ctcaaggacc tcctcgcgga gtccctggcg agccccggga gccgcttcga
      961 gttcctcacg gccatggcca ggctctgccc catcgagccc atcatcgacg agctcaccgg
     1021 cctccacccc gtggacctgg ccgacctcgt gatcgagggc ctgcccgcca agccctcgag
     1081 cctcgcgggc atcctctcgc cgcgcagctt cgtgatgcgc cccctcccca acgcctactt
     1141 catgcgcgac tccgcggccg tcttccgcga ctgggccgtc tcctgcgcca ccgccttcga
     1201 cgtccgcctc atcgagtcca tcgtgacgcg cttcgtcttc acccgccatc ccgagttccg
     1261 ggccaagggc ctcctcgtcg acgggcccag ggccaggaac cgcttcgtct ccatcgaggg
     1321 gggcgacatc ctggtcgtcg gcccccgcgt cctcgccatc ggcatctccg agcggacgag
     1381 ccccgacgcc gtcgagctcc tggcgaagaa cgccggccgc gccttcgagg agcccgtcac
     1441 catcctggcc gtcgtcgtcc ccaaggagcg cgcgaccatc cacctcgaca tggtcttcac
     1501 gatggtggac cgcgacgcgg ccctggtcta cgcgcccgtc gtcacggggc cgaaccgcgc
     1561 ggaggtggtg cgcatcgacg tcgatcccca cggccgcgcc tcctactcgg agaccgacgg
     1621 cctcctcccc gccctgaaag ccctgggctg ggacctggag cccatcctct gcggcgacgg
     1681 ccaccacctc taccaggacc gcgagcagtg gtggtcggga gcgaactcct tcgccttcgc
     1741 ccccggcaag atcgtcatgt actcctgcaa cgtccgcacc ctggacgccc tctcccgccg
     1801 cggcttcgcg gtcaagcgcg ccgtcgactt cgaggacggc cgcgaggatc ccttcgcccc
     1861 cggccgcctg gccgtgggct ttgacgggat cgaactggcg cggggaggcg gcggggcgcg
     1921 ttgcatgacg cttcccgtgg agcgcgagga gctttaggac tcttctaccg gactcttgga
     1981 gcctcacggg acggggcctg cccgcgccca tgcgtccccc ggcgcttcgc gcgggggcgc
     2041 gcatgggcgc gggcacccgt cgttccgcca tgccccgcgg gtgnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnngcgcggg ggcgcgcatg ggcgcgggca cccgtcgttc
     2221 cgccatgccc cgcgggtggg gggaaggggt cttgagtctc ctcgggcctc gtcgtgggga
     2281 gtggaggaga gttagcgctg cgtgggacgc tggcacgcgt ccggctcgcg ccggacggcg
     2341 cggggtgggg agcggcgctt cgtggaaggc ctcagcgggg gcgacgcttg ggacgccgca
     2401 cgcgtccggc ttcgccggac ggcgcggggt ggggagctgc acggaaggag agcttcgagc
     2461 tggggtcgct gctcgggacg gcgcacgcgt ccggctcgcg ccggacggcg cttgggggct
     2521 ctgggctcag gccttaggcg cgttggggcc gaggtcgaag ccgcgctcgc ggaaagcggc
     2581 gaggagttcg tccgtgaatg gttcccattg gccccacttg cgggagatgt gctcggtcca
     2641 ggagtagtcg gcgaaggtga agcggtcctc gcggccgtcc tcgagctcga tcttgttgcc
     2701 gagcttcgcg tagtagccct cagcgagata gccctcgtcc atggcgcgca tcgcgtcgcg
     2761 gacctcggcc tcgcccagct cggggtagcg gtcctcgaat aggacgaagg ggcgcggata
     2821 gcgggggcgc ggagcgagcc tctcctcggg gtagcccatg acgagctcga ccatgggaag
     2881 gacgcgcttc gggagcttgt actgcttcgc tatggcgccg atccgcttcg cgttgatgct
     2941 gccctcgccc aggaagcagg agcccattcc caggctctcc gcggcgatga ccatgttctc
     3001 cgcggcgtag gcggcgtcct gcatgcccag gaggaggagg gttaggtcgt tcgtcgcgac
     3061 cttccagccg cggagggcca tgaagcgctc gagcttgtgg gcgtcgacgc agacgacgaa
     3121 ccagaggggg gcgccgaagg gcgccttctt cttcctcgtg aggaggacgg agtagagctg
     3181 ggaggcgaag ggcgcctgcc ggcctgcgga gaggacggcc tcgatgacct cctccgaggg
     3241 ctgctccttc cggtacttcc ggaccgaagc gcggccgcgc aggacggaga ggaccgggtt
     3301 gtcgatcatg ggggctcctt gagtcctact tttatacgat ggcggacgtt tcgccgcaag
     3361 acgcttctgc cggtccttgc ggatcagcct gggccgcgcg agacttgagg tctcggcccg
     3421 gatcgttcat cccgggctcg aggaggagga acgcgatgct ctacagtctc tccacgttga
     3481 gcaaggagca gctcgagtcg ctccacgcgc tcgagacgaa gctcgggaag cccgtattgg
     3541 ccttctccgc ttaccgggac gagcccgcgg gcctttcctc ggccgagctt gaggccttgc
     3601 gcgagtccga gtcccgcctt ggcctgaccc tcgtcgccgt caaggcctag ccgcaaggcc
     3661 cggcgcgcgg ccccggccat tgtcgccgga gaacccgggg aaagtcccgg cgcgaagcgc
     3721 ccggggtgcg agtcgccaat tgtcgcctga gcgtataggc caagtcccgg cgcgaagcgc
     3781 ccgggctcag cgggcggagc agcccttcag cgtgaggtcg aagcttcata aagcctcgcc
     3841 ctcgtccgtt aggcgcctgc cctgaagcgc gagacgcggc gcttggccgc tcgcagccct
     3901 catcgcgcga accccgccgc cgctttttag gaaccagccg gagcggcggg tggccaagac
     3961 gaacgcgccc cggcgcgtca gcgcccgggg acgttcgtca tggccaggac ccgcccagcc
     4021 aggttcctat aaagcccgtg tcgaggctct gtgatcagct gcaaccggtg gtgctaccac
     4081 aggtctcgcg ccgtcgctgc gctccggcga cgccgaagac ctggcggtct tcggcgatta
     4141 tctgtagcct gaaatgctcg acctgcttgc ctcggtcaac tgcaaccggt ggtgctacca
     4201 caggtctcgc atttcaggca cgtcccattg cgcacgaggg tgaggtggcc gcagacgggg
     4261 cagggatccc cctcgtagcc cttgaggcgc gcgttgcgcg cgctctcctt gggcttggag
     4321 aggccggcgg cggcctggcc ggcgccggcc tggggcgccg aggcggccgc ggccagggcc
     4381 ggcgaggcgg cggcgggctg gttgagggcc gcgggggcgg cggagagcgg cgctggggag
     4441 gccttgggcg gagcctggac gccgcgggcg cggccgacct cgaaggcgcg gctcgcgggg
     4501 acgcgctcgc ctccgttgcc ccgcttgctc tgggtggccg tggcctccag gtcctcgggc
     4561 ttgatctgcc ccaggtcgcg ccggtccagg taagagatcg cgaggtcgcg gaagatgaag
     4621 tccaggacgc tcgtcgacat cttgatgtag tcgtggccct ggaccatgcc gttgggctcg
     4681 aagcgggtga aggtgaaggc gtcgacgtac tcctccaggg ggacgccgta ctggaggccc
     4741 agggagacgg ctatcgcgaa ggagttgagg atggagcgga aggccgcgcc ctccttgtgc
     4801 atgtcgagga agatctcgcc cagggagccg tcgtcgtact cgcccgtgcg gacgaacatc
     4861 gagtggccgc cgatcttggc cttctgggtg tagcccgcgc ggcggttcgg gaggttcctt
     4921 cggacgccgc ggggcgcggc gggcaggccc gggagctcgg gcgcgggggc ggcgaggccc
     4981 gtggcctgga cgcggagggc gctcgcggcc tgcgacgccg tcaccgccga ggccgccgcc
     5041 tcggcggagg cctccgcctc ctcgaggcct cccgactgga gggcgacgat ggagtccgcg
     5101 atgatgtcgg cgccgggagc cagcgcggag aggggctggg agagcttgga gccgtcgcgg
     5161 tagagggcga tggccttgag catcgagcgc caggccaggg agtaggcccc cttcacgtcc
     5221 tcgtaggtgg cggagttcgg catgttgatg gtcttggaga tggcgccgga gatgaagggc
     5281 tggacggccg ccatcatgga gatgtgcgcc ttccactcga tggccctggt gccgcgcttt
     5341 cccgaggggg tggccgtgtc gaagacggac tcgtgatcgc gcttgagtcc cggcgcccct
     5401 tcgacgccca tcgtgccgca ggcgaagagc tcggcctcct cgatttcctc gccggagaag
     5461 cccaggtggg cgaggaggct gaagcccggg aggccgtagg tggcctcggg gaccttcacc
     5521 gcctcgtagg cctccttccc gaggacgtag ggggcgaagc aggactcgag ggagatggcg
     5581 gtcttgaggg cggcctccgc cttgtccagg gcggcgaggg gcaggccctt cgccgcgagg
     5641 ctctcgtagg agagggcgag ggggcgcctt cctcccttgc cgtcgggggt tgaggcgccc
     5701 ttcagggtct cgtggcccag ggcgtaggtc tcgatctcct tgatcgcggc ctcggagtag
     5761 ccgagggcgc gcagggccgg ggggacgctc gtgttgatga tcttgaagta gccgccgccc
     5821 gcgagcttct tgaatttcac gagggcgtag tcgggctcga cgcccgtggt gtcgcagtcc
     5881 atgaggaggc cgatcgtgcc cgtcggcgct atcgccgtga cctgcgcgtt cctgtagccc
     5941 gacttctcgc cgatcgcgag ggcctcgtcc caggactcgc gggcggcccg ggccaggttt
     6001 tgcgggcact tcgcgggatc gagggcgaag gggcgcaccg tgaggccctc gtactcggcc
     6061 tccggcgcgg cgacggcggc gcggcggtgg ttccgcacga cgcggagcat gtccttcgcg
     6121 ttcgcggcgt agcgggcgaa gggcccgaag gcggcggcca tccgcgcgga ctgggcgtag
     6181 gcctcgcccg tgaggacggc ggccagggcc gcggcgaccg cccggccctc gtcggagtcg
     6241 taggccaggc ccatcaccat gagtagggag ccgaggttgg cgtagcccag gcccagggtc
     6301 cggtagtcgt agctcttccg ggcgatctcc ttggagggga actgggccat cacgacggag
     6361 atctcgagga cggtggtcca gaggcggatc gcgtgcctgt agccttcgtc gtcgaatttc
     6421 ttcgccttcg cgtcgtagaa ggccaggagg ttgagcgacg ccaggttgca ggcggtgtcg
     6481 tccaggaaca tgtactcgga gcaggggttc gagccgcgga tctcgccgtc cgcggggcag
     6541 gtgtgccatt cgttgacggt gccgtggaac tggaggccgg ggtccgcgca cttccaggcc
     6601 gcctgggcga tcttgtccca tagggcccgg gcgcggatcc tccgcatcgg cttcccgctg
     6661 atgcgcccga ccaggtccca ctcggcgtcc tcctcgaccg ccttcatgaa gcggttgtcc
     6721 aggcgcaccg agttgttgga ggactggccc gcgacggtgt tgtaggcctc gccctcccag
     6781 gccgtgtcgt aggcggggag gtccaggctc tcgtcgccct ggcggaagcg ggagagggtc
     6841 tggtggagga agggggcggg aaggccgccg cggagggcct cgcgggcggc cttgcgcagg
     6901 gcggggttct tctcgaagga gaagcggtcg tcgcccgcga ggccgccggc cttggccgcg
     6961 gacaggaggt cggcggcgta gcgcttgagg acggccgagc ccgcggcgag gcaggccacc
     7021 ttgtactcct cctcggcctt ccagttgacg taggtctcga tgtcgggatg gtcggcgtcc
     7081 aggatgacca tcttggccgc gcgcctggtc gtgccgcccg acttgatggc gctcgcggag
     7141 cggtcggcta tcttgaggaa ggagaggagg ccggaggaga cgccgccgcc cgagaggggc
     7201 tcgttcgcgc cgcggatctt ggagaagttc gagcccgtgc ccgagccgta cttgaagagc
     7261 ctggcctcgc gggtcacgag gtccatgatg ccgccctcgt tcacgaggtc gtcctcgatg
     7321 tcgaggatga agcaggcgtg gggctggggg cgggcgtagg ccgactcgct cttcacgacc
     7381 tcgccggtct tcgggtcgac gtagtagtgg ccctgggccg gcccctcgat cccgtagacg
     7441 gcgtggaggc ccgtgttgaa ccactggggg ctgttcgggg ccgccatctg gtgggcgagc
     7501 atgtagagga gctcctcgta gaaggcgtcg gagtcctcct cggagtcgaa gtagccggcc
     7561 tccttgcccc agagggtcca ggtgtaggcc aggcggtgga agacctggcg ggcgtcggtc
     7621 tcggccccgg gtttcttcgc gccgccggcc gcggcgccgc ccgaagcggc gagcccgcgc
     7681 tggtcggccg gcaggaaggc cttgaaggcc tcggccttgt ccgcgggcac ccccgccttg
     7741 cggaagtact tctgcgcgat gatgtcggcg gcgatctggc tccaggtcga ggggacgacg
     7801 aagccctcca tctggaagac tgcgcggccg tccggattcc ggatctcgct cctccgctct
     7861 tcccagcgga tgccctcgta ggggcccttg cccttcttgg taaagcggcg ttcgaatttc
     7921 acgagatccc ccttgcaagg actttaagga tgagggcggc cggccgcccg gggtttgcga
     7981 tcgatgcggg cttgaggccc ggtcactaca atatattggg gctggtcgat tttcaatacc
     8041 caagctgtag aatatttctc aatttaggtt ttagatgttt tgtggcccgg cgggccgtat
     8101 gaaggtaagg gcgcggcctt ttccgccgat aatcgggagt gaatatgaga accgccgcag
     8161 tagtctacgc aggatccttg agcgagtcgg cgcgcaagcc cctcgcgggg ggaggctcgg
     8221 cctatgcccg ggtcctcgat ttcgtcaggg aactccccgg cttcgagggg gccctcgtcc
     8281 tctccacgcc gggctccggg gacctccccg ctggccaggc ctcgggccgc ctccgcctgg
     8341 agaccaggga ggccttcagc ctctcctcca tcctcgaggc cgcggaaggc tacgccgcct
     8401 ccttccagga ggccggcggc ggcgaagggc ggggcggcct cgacgccctg gtcttcgtca
     8461 gggccgacga gcccctcctg gaccggagcc tggccgcccg catgctcgag aacttcaggc
     8521 gctaccgggc ggactactcc ttcgccgacg gctatcccct gggcctagcc tcggagatcc
     8581 tccacccccg ggccctgccc gccctgcgcc tcctcgccga gcgccacccc ctggagccgg
     8641 agcggggctg gctcttctcc ctcatccaga aggacatcaa ctccttcgac atagagacgg
     8701 agatctcccc caaggacctc cgcgaccggc ggctctgcct ggcctgcgac tcgaggcgga
     8761 acctcctcct cgtggagcgg ctcatggccg cgggcgtgtc ggacgccgcc agcgccctgg
     8821 aggtcatccc cgcgaggccg gagctcgagc ggagcctccc cgccttctac caggtccagg
     8881 tcgagggggg ctgtctccag tcctgcgccc tctgccccta tccgctcttc ggcggcgaca
     8941 tcctcaagcg gcgcgaccac atgacgcgcg accgcttcgc cgccctcatg ggagccatag
     9001 aggccttctc cggcgacgcc gtcgtcgacc tctccctctg gggcgagccc tccctccacc
     9061 ccgacatcga gggcctcgtc gaggaggtcc tctcccggcc aggcctctcc ctcatcgtcg
     9121 agacctccgg cctcggctgg agccgcggga cgctcgagtc cctcgcctcg cgctgggccg
     9181 gcccctcggg ggcgggcggc gcggcgggcc aaggcggcgc cgagggccaa agcggcccgg
     9241 gcggcgccag ccccaggctc aactgggtcg tctccctgga cgcccccaag cccgagctct
     9301 acgcgaggct caggggccca ggctacgagg aggccacggc ggcggcggag ctcctcctcg
     9361 agctcttccc caagggcgcc cacgtccaga ccgtcagggc cgtcgacaac gagccggccc
     9421 tggaggactt ctggaggggc tggaagaaac ggacggagaa cgtcatcgtc cagaagtact
     9481 cccgcttcgc gggcttcctc cccgagcgca aggtgaccga cctctcgccc ctcgtccgcc
     9541 ggccctgctg gcacctgaag cgcgacgtct ccgtcctcct ggacgggacg gtgccgctct
     9601 gccgcgactg cgtccggaac gagatcgtcc tcgggaagct cttcgacgag tccggcccga
     9661 acgacccggg ctccgtcctg gcccgcctgg aggccgcctg ggccgcgggc gaggcctacc
     9721 accgcctcca catcgagggg cgccatcccg aaccctgcgc gggctgcgat gaatactata
     9781 cgtacaacgc ttaacgacag ctccataccc tacaccgtgg tgggaggacc caaggggagg
     9841 tcccagggcg agggcttgcc cgtctccgtc ctcctcctgg accggggctc caggctctat
     9901 cggagcgagc tcttccgcga gctcgaccga ctcggcttcg agtccgtcgt ctccgtggag
     9961 gagggctccg aggccgtcga cgtcgaggcg ctgtcccagc gcttcccgaa ggtgcgcttc
    10021 ctcctcctcg gtgggaaggc gagcccgggc gagaagatca acctgggcat gcgcgagtcc
    10081 tcctcgccct acgtcttcgc gctctggagc gacatgcgcc tcgtggcctc cggcctctcc
    10141 ggacgcttct tcgagcgcct ggccgagcag aacctcctct gcctcgcgcc cttcctccag
    10201 gcgaagcggg gagagaccct gccgacggcc gccgcgccgg ccctggagcg gcagaacctc
    10261 cgcatcctcc ccctcgtccc cgcccgcgac ggggccttga gcctctaccc cttcgactac
    10321 gcgggcatct actcccgcga gaagttcgtg ctctccggcg gctacgacgg gggcatcgcg
    10381 aacccctatt ggcagaagct ggacttcggt ttccgctcct ggctctgggg cgaggagatc
    10441 cgcctctccc aggccctccg cgtcgcctac gaggaggagc ccccgacgga ggactcgacc
    10501 cccgacgagg actacaagtg gttctggctc aagaacctcg cgcccgtctt ccggggcgac
    10561 tccgcggcca tcccgccctc gcgctactgg gcctacctcc gccgccgcgg cggcaggccc
    10621 ttcgccgcgg cgagggaatt ccgcgcggcc gccgactggg tgcggacgaa ccgctaccgc
    10681 ttccgctccg gcgcctcgag cgtcgtcgac ctctgggagg aggccaagcc gtgagccgcc
    10741 cctcccgcct gggcctcgtc gtccaggcca ggctgggctc gacccgcctg gcgcggaagg
    10801 ccctcctgcc cctgggcggc ctcacgatgg ccgaccaggt cctgcgccgc ctcatcctcg
    10861 tgccagctga ggtctacgtc ctcgccaccg acgaggcgag cgcccccgag ctcgggccga
    10921 tcgccgcgag gaacggcttc gagctcctcg tcggcccggc ggaggacgtc ctggcgcgct
    10981 actgcctcgc ggtccggcgc ttcggcctgg gccgagtcct ccgcgccacg ggggacaacc
    11041 ccctggtcgc ccacgagctc gccgccacgc tcgtctcccg ggccgaggcg gcggaggccg
    11101 ccggggcccc gcccgcggac tacacgagct actcgggcct gcccctgggc atgggcgtcg
    11161 agctcgtgcg cgcggaggcc ctgctccgcg ccgaggccga ggcgagggag ctccccgagc
    11221 gggagcacgt ctgcccctac ctttacggcc acccggagct cttcaggctg gaccgccccg
    11281 aggcgcccga gccctggcgc ttccccgagg cccgggtcac cgtggacacg gcggccgact
    11341 acgaggctgt cctgcggctc tatggagccc tctactcggg cagccccgtc ccgacggagc
    11401 tcgtctacgc ctacctgcgc ggccagggga gggcggcatg agccgccgcg tcctcttcgt
    11461 cccctccgtc gcgaagggca acggctcggg ccacctcgtg cgttgcctct ccctggcgag
    11521 ggagctcggg cccggcgccg ccgtcttcgc gcccgaggcc aaggccgcgg gctcctggag
    11581 cgccgccgag ctctccctct cctattcccg ggaattcctc ggcgtccccc tcgtctcgga
    11641 gcaggcggcc agccaggcct gggacctcgt cgtcctggac aggcgggcca cctccgcgga
    11701 ggagctcgcc tactgggagc ggcttggtcc cgtcgcggcc ctggacgagg gcggacccgc
    11761 ccgcgaggcc gcttcctacc tcgtcgacat cctgcctcgt gccaagccgc cccgccgcgg
    11821 caagggccgc ttcgaggcgc ccaacgcggc ctccatcgcc tacctcgacc ttccccggaa
    11881 aaggcgcgag cctccgcgag ccttctcccg cgtccttttg agcttcggcg gagaggaccc
    11941 cgccggcctc ggtccggccc tcgcgcggag cctcgtctcc gagggcttct atccgccgga
    12001 gagcctgacc ctcgtttcgg gcgccctgcg cggtcccagc gacagggccg agggcctctc
    12061 gggcgtcacc gtcctgggcc cgatccagga cctaaaagag cacctggccc agtacgacct
    12121 cgtcttcacg cagttcggcc tgaccgcctt cgaggccgcc ttcgcgggct gcggcgtcgt
    12181 cctcctcaac ccgagcgcct accaccgcga gctctcggtc cgcgcgggct tccccgagat
    12241 cggcgtcggg aagccccgcg tcgccgccct gcgccgcctc ctctccgatt ccgaggggac
    12301 cctcgcgagg accaggcgca tcgcgcccga gtcgcgggcc tccctcgccg ccctcctcgc
    12361 gggcctggcc ccctcggggc cgaggagctg cccccgctgc ggctcgggag aaaggcgggc
    12421 cctcttccgc aacgcggcga agagctactt ccgctgctcc tcctgcggca cggtctacct
    12481 ccagcgcttc gagccgggcc gcgagaatcc ctacaaggag tcctacttct tcgacgagta
    12541 ccggcgccag tacgggaaga gctacctcga ggactggccg gcactgaccc gcctggcgga
    12601 ttcgcggctg gacctgatag aaggcctcgc ggagaagagc ctgggccggc gctcgggcct
    12661 ctccctcctc gacgtcggct gcgcctacgg gcccttcctc aaggcggcgc gggagagggg
    12721 ccaggagccc tacggcctcg acatggcgga ggacgccgcc cgctacgtgc ggaaggagct
    12781 cggcatcccg gcggcggcgg gcgacttcct ggatccggcc gtcgccgcgg ccttcggggg
    12841 gcccttcgat gtcgtgagcc tctggtacgt catcgagcat ttcgccgacc tggacctggc
    12901 cttgcggaac gccgccgccc tcctcaggcc cggcggcgtc ctcgccctct ccacgccctc
    12961 gggcgagggc gtctccgcga agttcgaccg cgagggcttc ttcgagcgga gccccgacga
    13021 ccatttcacg gtctgggagc cctcgcgcgc ggggggcatt ctcaaggcct acggttttcg
    13081 ggtagaactg cggaggatca cgggccacca tcccgagcgc tttcccttcc tcgcgggcct
    13141 gcccgcgggc tggccgcgca gggcggccct ctggctcgga ctcggggtct ccaggctttt
    13201 cggcctcgga gacaccttcg agatctacgc cgtccgcgag ccggccggcc cctcggggcc
    13261 ttccctccct tcgcggaagc ggcgggaaag agccagtccg cgttcctgag gaaggggtgg
    13321 gtttcatgac ggtcttcatc ctcggcgccg gagtcatgca gatcccggcg atccgcatcg
    13381 ccaaggagct gggctggcgc gtcgccgccg ccgacggcaa tccggccgcg cccggcgccg
    13441 ccctcgccga cctcttcttc cccgtcgacc tcaaggacag ggaaggcctc ctcgaggccg
    13501 cgcggggcct cgcccgggac ggcggcctgg aggccgtctt cacggcggga accgacttct
    13561 ccgcctccgt cgcctacgtc gccgggagcc tgggcctacc cggccacagc ctggaggcgg
    13621 cgaccaaggc ctcggacaag cccctcatgc gcgcggccct ggccgcggca ggcgtcccct
    13681 cgccgcgctt cgccgagttc cgctcggccc gggatcttcc cgccgccctc aaggccgcct
    13741 ccctggcctt tcccctcgtc gtgaagcccg ccgactccat gggggccagg ggctgccgcc
    13801 tcgtctcctc cctcgccgag ctggaggcgg ccctcgccga cgccctgccg cactcccgct
    13861 cgggccgggc catcgtcgag gagtacctcg acggccccga gttctccatc gacgccctcg
    13921 tccaggacgg gaaggtcgcg gtgcgcggcc tcgccgaccg gcacgtcttc ttcgcgccct
    13981 acttcgtgga gctcggccac acgatgccga gcgccttcca gaaggaggtc ctggacgagg
    14041 tcctgcgcgt cttcgaagcc ggcgtgaagg ccctgggcct cacgcggggg gccgcgaagg
    14101 gcgacatcaa gtactgcccc ggccgcggcc gggcctacgt gggcgagatc gccgcgcggc
    14161 tctcgggcgg ctatatgtcc ggctggacct acccctacgc ctccggcatc gaggtcacgc
    14221 gcggcgccct cgagctcgcg gccggcctcg ggccctcgga cggagccgcc gaccggggct
    14281 gggtctccgc cgagcgcgcc ttcatctcga tcccgggcag ggcggcctcc gtcctggggc
    14341 tccgcgaggc cgaggcggcg ccttacgtga agaacctgtt tctccgcgtg gccccgggcg
    14401 aggcctgcgt cttcccctcc aacaacgtcg agaagtgcgg caacgtgatc agccaggccc
    14461 cgagccgcga ggccgccgtc tccgcggccg agggcgcggt caggggcatc ctcgtccgtc
    14521 tccgcccggc cgaggccgcg acagaggcct tcctacgcgg cgagggccgg gtcgccaacc
    14581 ccgacggcgg cgcctggccc ccggaggcct tctcgcctgg gccgcgcctg gcggccttcc
    14641 tggccgccct gcccgagcgc tcggacgagg cggacggcgg actgaagcgg gccggagccg
    14701 aggggcttgc ggcctccgta aggctactcg tcgcgccgct ccccgaggag ctccggacgg
    14761 aggccgccac catgagcccc gactggtcgg gccgcggctt cctcgagtcg gccgagctcg
    14821 ccctccgcct gggcggcgcg cgcctcgcgc cggaaggggc tcccgccctc gccggccgct
    14881 tctggcgcgc cctggcccga ggcggcgccc aggccgcgct ctacgtcctc gacaccgagg
    14941 cgcggggagg ggagggggcg tgaggcgcga gccccgcgcg gccttggccg cctgcgcttt
    15001 tctcctcatc ctggtcggca gggcccctgc cgcggaaggc tccgccgttc ccgccgccaa
    15061 gcccggctcc gctcccgccg catcatcccc cgtaccggct cccgcgccgg ccgccgcgcc
    15121 ggtgaaggcc aaggccgagc tctcggcgcc agccgcggcc gagcgcctcg gggccaagct
    15181 ccgctgggac ccgctcacgg gcacgggcag tttcgagcgc gggggaaatc tcctttcctt
    15241 tgccctgggc tccccctacg ccctcttccg ccgcgaggag ctcatcggcc tcgacgcgcc
    15301 gcgggacggc ggaaacggcc ccctcttcac cgaggcggcc ttttcggcca tcgagggagg
    15361 cttttcgagg gccgaggccg cgaagaagtc gcacttctcc atagcggcca tcctcatcga
    15421 cccgggccac ggaggcaagg actccggagc cgtgggcgag cacgcagtcg gaggcaagaa
    15481 gctccgcgtc gtggagaagg acctggccct ggacctctcg ctccgggtct accgggccct
    15541 gaaggccgcg taccccgaca ggcgcatcct gataacgcgt tcgggcgaca gctacccgag
    15601 cctggaggac agggtctcca tggccaacga ggtggagctc gaggacaacg aggccataat
    15661 ctacgtctcc gttcacgcca acgcctcctt caacaagaac gcgaagggct tcgaggtctg
    15721 gtacctcaac cccgagtacc gcaggaccct cgtggcccag gacaaggcca aggacctggg
    15781 ggaggacata gcccccatcc tcaacgccat gctcgaggag gagttcacga cggagagcat
    15841 cctcctcgcc cggagcatca tggatggcct ttcctcgggc atcggccggg agagccccaa
    15901 ccggggggtc cgcgccgagg agtggttcgt cgtaaggaac gccaagatgc cctccgtcct
    15961 cgtggaggcc ggcttcgtca cgaatcccga ggaggcggcc ctgctcgcgg acgacgatta
    16021 cttgcggcgc ctgtcggagg ggatatatac tggcatagtg gatttcgtga gctatttcga
    16081 gaacatgaag gggtcacaag cgcgatgaag aagttcctcc agaaggccgg ccccttcctg
    16141 cggcgcgtcc tcgactggct ggccccggcc gaccatctgg ccctggccgc cctcggcctg
    16201 gccttcctcg tctcgctcgg ggcctggagc ctgggcgacc gcctggagga cgcgaccctc
    16261 tatttcccct cctaccgcac aggatccctc cgtggcgaga cgcgggccct tccccgggcc
    16321 cggggcgcgg aggcgcgcgc cgagctcgtc gcctccgagc tcctcctggg cccgggcgcc
    16381 ccgggcctcc ttcccgcctt tcccgccggc gccagggtcg agtcgagcgt cttcaggaag
    16441 gggaggctct acctggacct ctccgaggac gccgcccttt cggaccgagc ggacctggag
    16501 aggggccttt ccgccctgag gcgcagcctc aaggccgccc ttcccctcgc caagggcgtc
    16561 gtggtcacga taggcggccg ccttccctac gccgactatc cggagccgga gcccccggcg
    16621 gacggcccgg agaccgcccg ggccggggcc ggggccgaaa aaaaagggaa aaagaattga
    16681 caaaggcatc gcggcgcgta tacttcctaa ctaattaggc taaaaccgaa aacgcgcacg
    16741 atgaaaggag ccatagtaat gagaaggcag attatcgcag tggcgctcgc gcttctcttc
    16801 gtaggtgcgg ccgcgctctc cgcggacgag gccgtcctca tggatttcag cctcctcaag
    16861 gcggacatca tcgccgaccc ggtgaaccag ggaaaattcc ttgagaaccg caacacgatg
    16921 atggacttct ccggcatcgc cggcgccggc tacaccgagg agcagaagaa gcagatgagg
    16981 gtctccctcg cgatccccaa ctgggacgtc gtcctcgctt cctcctcccg gaacaacatg
    17041 aaccagaccc tctccatgac cgccgaggcg accgtcgccc aggacgcgaa gcagttcggc
    17101 ggcaagacgg tcctcggcat gcgcgtccgc ttccccaccg agcccttcaa cagctgggcc
    17161 gcggtgaagc ccccctacga gatccccgcc ttcgagccga aggccgacat cgacgaccag
    17221 ggcaacatca ccccgaagaa ggacgcccag ggctccgacc ccgtcaacgc ccgcatgtcc
    17281 cgcttcgagg gcaactacga cgccaacacc aagatcacga ccgccctcgg catcgtgaag
    17341 aacgtgggca gcctcaagac cgtcgccgtc accgtgaagg gcctcaactt cccccacggc
    17401 ttctccgtca tcctcaagga ttccgacggc aacgagcgca acatgttcat gggctacctg
    17461 aacttcgacg gctggaagga gctccgctgg gacaaccccc agtacgtgac cgacgtccgc
    17521 aaccgcgagc tcaggatcta cccgctttac ccgaagaccg tccccttcat caagctcgac
    17581 ggcttcctcg tgacccgcga cgcggccaac gagggcggcg acttcgtgac ctacgtcaag
    17641 gacgtcaaga tcctctatga caaggccgtc ctcgagcccg tccaggacat cgacgacgag
    17701 gccatctggg gcatcgtcgg caagaaggag tccgagcgca agatcctcga gtccaggcgc
    17761 ttcggcagcc agcaggtcat gcgctacatc gagcgccaga agcaggagac caagtccgac
    17821 ttcactcccg ccaccaagta aggcccgcaa gagcctgtca ttgggcgaca agggccggaa
    17881 cctcctgggt tccggccctt tttccttttc cgccggcctc tcccgttgta tgattggggc
    17941 atgagcggag aagctaccga cccgataccg gaccgcggcc tcctcgaatc ggactacgag
    18001 cgccggaagt cctcctacca ggcggcgata gaggacatgg agcgcctggt gcgcgacagg
    18061 ctcgaggccg agggcctccg ccccaccatc aagggaagga tcaagtcctt cgacagcctc
    18121 tacaagaagc gcatccgcct cctcaggcag gccatggcct ctggcgccga gcggcccctg
    18181 cccatcaacg acatcatcgg cctaagggtc gtctgcccct tcctgggcga cctggcggag
    18241 gccgagcgga tcctctcccg ggacttccgg gtgcttgaga ccgagcgcaa gggcgccgac
    18301 cgctccttcc gcgagttcgg ctacgagtcc atccacctcc tcgtggccct gccgcccgag
    18361 ctcggggccg cgcgctcggg cctggacgtg gacgcctgcg agatccagct ccgcaccatc
    18421 ctccaggagg cctgggccga ggtcgagcac gagctcgtct acaaggccga gttcaccccc
    18481 ttcgacgagc cgatgaagcg gaagctcgcc gccctgaacg cgaacctctc cctgtccgac
    18541 atcatcttcc aggagatccg cgagtaccag cgcaggctct ccgaggaact cgcgaggcgg
    18601 cgaagcgcct tctacagcaa gatcgaggag gccatcgacc agcccttcgg cagcgccatg
    18661 gccgacggac gcccgagccg cccggccgcc tgccaggacc gctccgtgga cgaccgctcc
    18721 gccgcggcct cgctggacga cctcctcctc gacgcgctct acgcccacaa ccggggcgat
    18781 tatgccgagg ccatagcgat ctacgggcgc atcctcgaga attcccctcc caaggaggcg
    18841 gccgccgtcg tgaccaagca ccgcggcatg gcctatttct cgcagtcgcg ctacgaggag
    18901 gccgtccagg acttctcccg gaccctcgag ctggacgggg actgctacaa ggcagcctat
    18961 ttccggggag tagtccggag cgtccagggg cagtacggaa aggccgtgga ggacttcgac
    19021 ctggccctgc ggatcaaccc ctaccagttc ttcgcgatct accgcagggc ccaggcctat
    19081 tggcacctgg gcgactatcc aaaggtcgtg gccgactgcg agaccgcgaa ccggatcctg
    19141 cccggccgtc ccgaggtcat ggagctcaag tccctcgcga tggagaagct cgacctcctc
    19201 gcatagcttg acagcataag gcaattctca ctatagtcag cacgaacgtc cccatcgtct
    19261 agaggttagg acagcgggtt ttcatcccgc caacaggggt tcgattcccc ttggggatgc
    19321 atcgattcga agccggctgc ttggccggct tccttatttt ccggccccaa ggggaatcga
    19381 agcgcagcgc cgcgctcgaa gcgcgcgaga catcgagcgc gcttcgggcc gaggcgcagg
    19441 atgtgccgag aggcgcggag ccggcctgga gcgagcggcc aggacggccg cgagcggaag
    19501 gcggttcccc ttggggatgc atagactgga agccggccgc aaggccggct ttcttattgg
    19561 tggccccaag gggaatcgaa ggccgcgatg cgcgccgaac ttgacttcga ccgccaggac
    19621 gggcaagctt gtcggcggca tgaacgaagg cgaccggatc gggaactaca ggctcgaagc
    19681 caagctcggc tccggggaga cgggctccac ctggctggtc cggagcgtcg cgaccgatcc
    19741 cctaacggga cacggcgagg ccgtgctcaa gatcctggat ctcggggaag cgagaggttg
    19801 gtcgggctat gacctcttct tgcgcgagat cgaggccctc aagaaccttt cgcatcccgg
    19861 catcccgcgt ttcctggact gcttcgagga tttcagcgaa gggggcgcgc gcttcgccct
    19921 ggcgatggag cggatgccgg ggcaggccct cgagagcctg gctgccgccg gccgccgttt
    19981 ttccgaggaa gaggtggcgg cgatcctggc agccctcgcc gacatcctcg actacctgtc
    20041 ctccctccgg ccccccgtgg tccaccgcga catcaatccg cgcaacgtgc tcttggggcc
    20101 cgaggggaaa gtctccctcg tggacttctc cggcgcccag gaggccgtgc tgcgcggctc
    20161 aaggcccgga gccaccctca tagggacggc cggctacatt cccctagagc aggtctcggg
    20221 caaggccacg gtgcgttcgg accgctacgg cgcggcggcc accgccctgt tcctcctcgc
    20281 cagggccaat cccgccgacc tgcccctgaa ggacctcaag cccgacctgt ccagcctccc
    20341 ttccctcggg cggggactcg ccgcgatcct ctcctcatgg ctcgaaccgg acgaggccag
    20401 acgcgacctt gagccggccc tggcctcccg catcctccgc ggcaagactc cggtgccgga
    20461 acccgtcgtc gaggcgccca ggaaggcggc atcgaggggc ggcaagggcg gcgcggcgag
    20521 ccttccttta aacagcaggg tgaggatcga ggaaggaccg gaccggctca ggataaccat
    20581 tccgcctttg gggctcggca cggccgcttc cctgggggcg ggaggttttg tcctattctg
    20641 gctggggttt gtcggcttct ggaccttcag cgcggcggcc atgggagctc ccctcgtctt
    20701 catccttttc agccttccct tctgggcggt cgggatcgcc ctcggacgcg cggtagcgct
    20761 cgcggctttt gcccgaaccg aactggtcct ggacaggaaa tcgggcctgg cgctctctcg
    20821 gcgcttgctc ggaaagggca ggggcaggca ctgtccggcc gcggaccttg gcgagtgcgt
    20881 catcgtcaac tcccccgtca ggatacaagg aaggccccag cggcagctgg ccatcgaggc
    20941 cggggccgag agcctcagga tcgggacggg gctgtccgaa cgggaactcg acgccatccg
    21001 ggcgaaaatc gaggcttggc gcggggaatt catgcgctaa gagtggcggg gcgcaagacg
    21061 cgcctcgccg gcaggccttg cgcgggccag aagcttgcca ggataccgag cattgagtat
    21121 cattcccgct agataaactg tcgccgctcg cccgagcctt tggcccgggg aggcggccca
    21181 tggaggtacc atgaagaaga aagtcctgct cctcgccctc gtcgtcgccc tcgtcgcggg
    21241 cgccaacgcc ttcgcctaca gggcggccgt cggcggagag ttcgccatga agatcggcca
    21301 gggcctcccc aattcggccc tcctctcctt caggctcccg agcttcccgg ccgtcttcgg
    21361 actcggcctc acggtcggag agtccacctc ttccctggcc ctcctcgccg actggtggct
    21421 ctaccagggc accctggtcg actccgtggg cctctacgtc ggccccggcg ccttcctcgg
    21481 cgtctcggcc gacgccttca ccctggggct ccgcgtcccc gtgggcatca acatctaccc
    21541 gatcaagccc ctcgagctct tcgtcgagtt cgcccccgcc gtcaccttca tcgccccctc
    21601 gggcgtgcag atccccgctt ggggcgtcca ggccggtttc ggcttcaggt tctggttcta
    21661 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacgatgga gccgcccacg
    21781 acgaggccga ccgccgcggc ttcctggaat tccccgctcg tgacgccctc ctccttgcag
    21841 cggatggtgt ggtagaggat gcagtcgtcg cagcgcagga ccagggaggc gacgaggccg
    21901 aggagctcct tcgtcttggc ggagagggct ccgtccctgt aggtctggcc gtccaggctg
    21961 aagaagcgct tgatctccag gccggccagt gagaggacct tctcgttgag gatctcgcgc
    22021 tccgcctgcc attcctcgac gctcttgtcg aaaagctttc catcggacat cgttcgcacc
    22081 tccggggcaa aaaggataca tcggggactg cggagcggta aagcgcgaag accactggac
    22141 taaatatatg gaaaaacata tattatacct gtgccacaaa aggagggaca aatgtcggaa
    22201 aagctgacga aggatagttt caaggcgaaa atattcgact gggaaaaaaa caaggaatgg
    22261 aactatgcag gcgaccttcc cgccatcatc gacttctacg cggagtggtg cggaccctgc
    22321 aagatggtcg cccccgtcct cgaggagctc gccgccaagt acgagggcaa gctcgccgtc
    22381 tacaaggtgg acaccgacgc ggagcccgag ctctccgcga tgttccagat ccagagcgtg
    22441 ccgaccctct tcttcgtccc caaggtcggc gagccgagct tcgccctcgg cgcccttccc
    22501 aagaaggatc tcgagaaggc catctccgaa gtgctcaagg tgagctaaag gcgctctccc
    22561 cgacgccaga ggctcaaggc gccagccggc tcgtcccggc tggcgcctct ttttatggaa
    22621 cccggcggcc taggcggagg gggcggccac aggcacgccg aagggcacgg cgagcttggt
    22681 cgcctccttg agcgcctgtt cgaggagggg gccgagctcg agctcctcgt aggccttgga
    22741 ctccctctcg acctcggccc tgaggagggg gtgcttgggc gagaagagga cgcagcagtc
    22801 ctcgtagggc aggatggaga tctcgaagct gccgatcttc ctcgcgagct cgatcgtgtc
    22861 ctccttgtcc atgccgacga ggggccggaa gacgggaagg gagacgcgcg agttcgtgaa
    22921 gcgcaggttc tccgccgtct ggctggccac ctggcccagg ctctcgcccg tgacgaggct
    22981 gttcgccccg acgcggaagg cgagcttctc ggagagctcc atcatggcgg cgcgcatcat
    23041 cagggtcgtg aggtcctcgc gggcgcgctg cttgatccgc atctgggtct ccgtgaaagg
    23101 cacggtccag agggtgaggc cgcccgtgta gcccgcgacg accttggcca gggctatgac
    23161 cttgtcctcg gcctccttgg aggtgtaggg gtaggcgtgg aagtagaccg cgtccaggcc
    23221 caggccccgc ctcgccatga ggtagccggc gacgggggag tcgatgcctc ccgagaggag
    23281 gaggaggccc cgcccctgcg agcccgaggg caggccgcgg acgcccttct cgggatcggc
    23341 gtagacgtag gcccgctcgc gtatctccgt gaagaccgtg aactcggggg cgtggaggtc
    23401 gaccttcgtc ccgggcaggg cctcgaggac gcgggcgccg atgtcgcggg agagctcgta
    23461 ggagtcgagg gggaagctct tgtccgagcg gcgggactcg gccttgaagc gcgccttgcc
    23521 ggaggcggcc gcctcgcgcg cggcctccac ggcggcggcg aagacggcct ccggcttctt
    23581 ctcaacgcgc cgggcccggg cgaagccgtt gatccccggg gtgcgggcca ggacggcctc
    23641 gacaaaggcc tcctttccgg gctcggcctc cacgaagata cggccgggga agtactcgat
    23701 ccgccggacc gagccctcca ggcggcgctt caggtcggcc ttgagcctgg cctcgaactc
    23761 cttgcggttg ccgagcttga gcagtatctc cccgatcttg acgaggtaga ggatttccat
    23821 ggacgggact ccttaggttt tcaatcgacg gtaaagggcg tcagccgtct cgagaaagcg
    23881 gtcgatctcc tcaggcgcgg tcgcgggccc cgtggagacg cggatcgcgg agaaggcgag
    23941 gccagcctcg aggcccatcg cctccaggat ccggcggccc ttcttgcgcg cgttggcgga
    24001 acaggccgcg ccggtggaga cggcgacgcc ctcgtcggcg agggcgcggg cgaagacctc
    24061 gcctgagagg ccggggaagg cgatggagag gatgaagggc gagtagcgcc cgtcgcccgc
    24121 cgtcctgccc agggggagg
//
LOCUS       DAOOSJ010000009        23792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1476_length_23792_cov_7.260437, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000009 DAOOSJ010000000
VERSION     DAOOSJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 23792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23792
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_23792_cov_7.260437"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..369)
                     /locus_tag="PLG14_01075"
     CDS             complement(97..369)
                     /locus_tag="PLG14_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOX47747.1"
                     /translation="MDHHSHESGEAVAALKTARGQVDAVLRMIEDDRYCIDVSKQILA
                     AVALLKKANIVILKQHMNTCVKEAFVEDKGAEKIEEIGLILEKYIG"
     gene            complement(450..2153)
                     /locus_tag="PLG14_01080"
     CDS             complement(450..2153)
                     /locus_tag="PLG14_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOX47748.1"
                     /translation="MTIKRTKLRIGGMTCPSCQARIQAALAGLQGVESVEVSLERGEA
                     TVEHGLEDLAVLEAAVEGAGYSVLPSGLAARAAELLPALALGLLLSGAYLAAGAAGLF
                     NNLPRIDSSLGYGALFVVGLLTSAHCVAMCGGIALSQSLGSAGARESGGEARTGAAPG
                     RLERLGPGLLYNGGRILSYTLLGGLAGALGSALDFSPAAKGAVTGLAAILMLAFGLSS
                     LGLFPRPSRPGRFLPKAAGRAAARAAAALRKRGPFAVGLLGGLMPCGPLQTMQLYALG
                     TGGFLPGASAMFVFALGTAPLLLGFGLAAALMPLRRLSLMRKTSAVLVIFLAFVTFGR
                     AAALAGIALPGLALAGRDPASATLSRPRGGDRGGATAGREGAAPLLAAGVQPRLTAAP
                     SAAALAALPRARLDGGVQSITTEFGANRYQAFVVQAGVPLKWTIRVRAEDLNGCNNPF
                     VVPALRLKKTLVPGDNLVEFTPGAPGAMPYSCWMGMIRSRIEVVASLADAPTADAPSA
                     RLAGFGGPLAASALPSGVGGSPSGLAEAGLPTCCGLAAAGGKADPETGPALPLDLRSV
                     P"
     gene            complement(2282..2869)
                     /locus_tag="PLG14_01085"
     CDS             complement(2282..2869)
                     /locus_tag="PLG14_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOX47749.1"
                     /translation="MSRAFVDEDDSTAGESEAPEIKIPIPQGSRNYLTPEGAERLSRE
                     LHELVQETRPRILADVARMAGADKDELAALRRSLGLVERRVEYLSMMSSLAEVIPPPE
                     GGYDRVKFGARIRVREAPGGGAAEAGEGPRGRPGAAGAESEYRIVGVDEAEPERGLIG
                     WTSPVARALMGKRPGEVALVRLPAGERRLEILAVE"
     gene            2982..4442
                     /locus_tag="PLG14_01090"
     CDS             2982..4442
                     /locus_tag="PLG14_01090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX47750.1"
                     /translation="MTEPFVFRSPTRLVFGAGSVESAGAEALAAVSAFQAAAASSSAA
                     SPGGAGLPGGGSPRGAAERRRLAFLVTGKGHTARSAGLARLRASLERSGFAVELYAEA
                     TADPPVELVLACAEGLAASGASLVVAYGGGSPIDCAKAASVYAANTAPQGPFPGAPFL
                     DFLYGRLVFSAPGLPLMAVPTTAGSGSEMSAAAVTTDLAASSKRGLSSDFFFPRAALV
                     DPELQATMPPALAAATGMDALTHAIESFASLKATPLTRAIAGESARLIAGSLERAVAA
                     AAEGPGGAADLGGAEAAGAAAIGGAGGAAARAAAAARADMALASAQVAMAFSQTGLGM
                     VHGFAHPVGARAGVAHGVANAVLLPYVMEACAEAAPGPFALLGDLFAAASPSGEAEVR
                     AAGEGSLGARRGGLASGLRAPDRRAPEAERAAYAVGFVRELSRAIGIPARLRELGVDR
                     GDLPAILADAISYRGRGGSPRAFTDAELGGLLERAY"
     gene            complement(4560..6083)
                     /locus_tag="PLG14_01095"
     CDS             complement(4560..6083)
                     /locus_tag="PLG14_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOX47751.1"
                     /translation="MSVPERFAAVVAGGGPAGLFAAITLAEGLRARRARIDAGTGAAM
                     AAAPAEAHPRGDGAAGDGGTGAAPAGPPPRVLVLERRERPARKLLLSGSGQCNLTHAG
                     PMEDFLPRYGGGGKPGAAGRFLKPALYALANEDLLDWFRARGLGFEAEAGGKVFPATR
                     RASSVLDLLLSECSRLGIVVATGRRLLSAERVGEGQSGGPGADGEGRAAADAAGAMRG
                     PERAVAAGGFLLRAARTGPEGSGAEESYAAPLLLLATGGASYPATGSTGDGYALAKAL
                     GHSVVAPRPALAAVRAKGFRLEPLAGLSFRGAGLALRRGGKRVLAGEGDLLITHEGLS
                     GPLVLDSSRGMEPGDTLELRFADVGLEEFRERFDRLLAKEPRRLARTVLAEAGLPKAM
                     AELFCSLAGLAPETAAADLRRESREALCRLACECPVQVGSLSGWDEAMATAGGVDLAE
                     VSPKTMESRVAPGLFFAGEVLDFDGDSGGYNLQAAFSTGALAGRAMLAALAGLAGGA"
     gene            6214..7107
                     /locus_tag="PLG14_01100"
     CDS             6214..7107
                     /locus_tag="PLG14_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47752.1"
                     /translation="MDSVAPKAVRRRFGDRYDGRRLRSLSPFYQITPYIMRRRSDAQD
                     YFEDRIEIGAAEAWLRRLRDGGRPDIGFLHLFIAVLVRTMALRPRLNRFVAGQRIYAR
                     NEILVSLALKKRLHEDSPETTVKMAFLPTDTIFDVADKVNAAVEANKAAESSNQTDRA
                     ARLFMLCPGFLVRFLVWGLRCLDYAGLLPKAIHRASPFHASAFVTDLGSLGIRPIYHH
                     LYDFGTTSIFVAFGSKERGRELDEEGLPRARKHISVKIVNDERICDGHYYASAFKYML
                     GLFRRPEELETRPPSVARDVD"
     gene            7124..8722
                     /gene="sbcB"
                     /locus_tag="PLG14_01105"
     CDS             7124..8722
                     /gene="sbcB"
                     /locus_tag="PLG14_01105"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOX47753.1"
                     /translation="MARSFFWYDLETFGLEPRYDRIAQFAGLRTDENLEETGERIILY
                     CKPTPDYLPSPQACLVHGITPQAAMEEGLSDYGLALRLRRELMEPGTTALGYNTLQFD
                     DEFLRSLFYRNFFDPYEREWRNGNGRWDLVDLMRAMRDLRPGGLVWPTGEGGRPIFTL
                     EALAKANGVAHKSAHDAMHDVLATLGLARLAKARQPRLFDWYLSHRTRDSLRPLIDLV
                     ERSPLVHTAAAYTSERGCTTLVAPISLDPENRNQLLAIDLRYDPAEILDLPVEELRRR
                     VFTKAEELEVPRLPLARLRLNRCPFLAPRAALSPEAAERLGIDAAACEAHLSLIKKEP
                     ELIQKLSAVFEGPLPPSEESDPELRIYSDGFFPEEDAEVFAALHEALAEALAGAEGAA
                     GAGLAASGAAPAGAAAAEGSGAAPGGEPAAGTASALLETKARFYRRRFRDDRPPQMLR
                     RFFARNFPETLGPEEARRWRGFCASRLQLPPVEGVADLATFSKFVGQRLETPSLPSRD
                     KAILLALLDYRSRLEREVLDYPGA"
     gene            8810..10063
                     /locus_tag="PLG14_01110"
     CDS             8810..10063
                     /locus_tag="PLG14_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX47754.1"
                     /translation="MKASIRRTLAVAAIALCALPAMLAAQSGEIVLKWPCIWVGKDSK
                     APAIAEIVDSFNKANAGKIRVEVEPQPDYNAYEQKVRTSLAAGVAPGDIFTLKLNATT
                     KEFYGSKYIMDLTKDFSGSWKASFDSGAVAQSTIDGKLKTLPFETAVLPIWYNMDAMR
                     KAGVSAPPATMPELWTALDKLKASGIYPMSQMTGDTNAWTSMIWFSHFAVSFGGAKVW
                     EKPFTDKAFVESAKLMQRIFKDYTTPDAVGAGAGVSGGHFLAGRTAIFSNGPWYAGRA
                     DLRATAFFPSVTIAAAPPAGSTKGVMISRLQANVAAGETKDKNRRAAIVSFLKYLTAP
                     ENISKLAQGSGAMFAVKSGYVPTDALQKQFYDIAAASKTTANDLEAALGSEATLEFAQ
                     QLGALVLGNITPEQFCALVEKKIDR"
     gene            10147..11031
                     /locus_tag="PLG14_01115"
     CDS             10147..11031
                     /locus_tag="PLG14_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOX47755.1"
                     /translation="MKKRRNPNALKIALFLAPVLVLFAAFFAYPFVFTIAASLTKWRG
                     IGAMRFIGFGNFGKLLGDQTFRLAIRNNVVWALASGFVQVPLAVLVALILAREPRGWK
                     VLRTVYFLPNVISTVALAMVWTAMYNPQYGALNGLRELLGLEARNWLGDPATALPAVI
                     LQGVLYIGYFMIIILAAAMNIPRELYEAAEIDGATTLQQEFRITLPMLRGSIVTAMTL
                     AMAYGMRHFEATYLMTGGGPAYSTTTMGIDLFLKMDALRYSEASAAGVILIVLGTVVI
                     VAIRRLLGRTDPMAEAGQ"
     gene            11050..11892
                     /locus_tag="PLG14_01120"
     CDS             11050..11892
                     /locus_tag="PLG14_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOX47756.1"
                     /translation="MRAKNKALSLGAGLLKWTVLLFFLAVALLPLAWLVLNSFKTNLE
                     LQSSPFSLPARPAFENYANAIAMAELPRLFVNSVLVVVGTVFLNLLVSSMAGFVLARE
                     AWKGRDTVRTVLEAGVLVPIIAFMVPYFTLVTKLGLYDKLYTLILVYAAINIPVSIFL
                     VTSFMRSIPKELEEAAIIDGCGFFQRYSKVILPLSRSGLVTAGTFCFIYAWNEFVMAM
                     LLTSSIHSRTIQLGIRFFTSQFITDYAGMFAAIVLSMLPSVLAYVFLHNKIIAGLTAG
                     AVKG"
     gene            11897..>12090
                     /locus_tag="PLG14_01125"
     CDS             11897..>12090
                     /locus_tag="PLG14_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47757.1"
                     /translation="MSDLEIVRIPGGFEARLRGRLVLAHRPGEPLLRAGRGEARFEMY
                     RGNFEVSERLDELVALEDCGL"
     assembly_gap    12091..12190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12191..13981
                     /locus_tag="PLG14_01130"
     CDS             <12191..13981
                     /locus_tag="PLG14_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOX47758.1"
                     /translation="PLWTSEQGVGRNKTTEVTRLADLHDKAGGDYWWTFFPEPTFATS
                     RRLSYHLETSAYAAFDFRSPGRHELYAWALPGRLVIGAADTMRGLVSGVSALLGRQPE
                     LPDWAYDGVVLGIQGGTEACLSKLERARKAGVPVAGIWAQDWEGINMTSFGQRLRWDW
                     QWDRERYPGLPAEIARLRAEGVRFLGYVNPYVAAGKPLYAEASAQGLLARRADGSEYL
                     VDFGEFDAGIVDFTNPAAVEWFKGVIKKNLVGIGLSGWMADFGEYLPTDAVLFDGTPA
                     EIAHNAWPAVWARVNREAVDEAAAEAAAARTAAAPGAPPVAGARPPASGAAAASAASA
                     GGAAAAPSAAPAFDPAECLYFMRAGFTGSQRDCPLMWAGDQNVDWSKDDGLPSVIPAA
                     LSLAVCGHGLHHSDIGGYTTLFGMRRTKELFLRWAEQAAFTPFMRSHEGNRPKDNWQF
                     DSDEETLAGLGRMGRLHAALKPYLKACVGENAREGVPVMRPLFLHFEEDPEAWSLEDE
                     YLLGPDLLAAPVLEEGAARRRLHLPPGAWIHLWSGSRLEAPAPGGASVEVEAPLGEPP
                     AFYREGSLWTATFAAAAAAARAARGSAPGR"
     gene            14040..15218
                     /locus_tag="PLG14_01135"
     CDS             14040..15218
                     /locus_tag="PLG14_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOX47759.1"
                     /translation="MSPKDNPLRAADVRERNEKIVLRLIRGAGARGLSQSEAVVATGL
                     RAPTIFRIFSSLEEEGYVEPLAPASAEEEGRERKGRPRVSFVARADALYAIGAEFWVD
                     RVSLGVFDFRGERVASSVASLDKGVDAEAVVEAIARCAEGAIESLGLSKDRVLGLGLG
                     APGQVNVRAREIAFYSRIPGLRDFPMAARLEDRLGLPVSIHNNCSIIALSEFRYGGLQ
                     AADSLFIFLLRSGVNGAFVDGGRVYLTSDGTTIESGHISVDYDGEPCACGARGCLETY
                     VTALDGPNLESGKWLFEGLGPALEAGDPKAEATLREAALYIASAARTVSRLFRPRAFL
                     IVAVSDSVSEALARLVRERLESSASGFDRPAPLFEARAYDPGLAQRGAADLVLDDFLG
                     "
     gene            15358..16674
                     /locus_tag="PLG14_01140"
     CDS             15358..16674
                     /locus_tag="PLG14_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOX47760.1"
                     /translation="MKQHIKLGLVCLARETFDFNAAAEIYGRIRGELASLENVSWEMV
                     SELVISVEDAKKAAARLASASLDALVCISGTFHLGHLVLEIRKRNPLPILLWGLDELP
                     YNGGKIRLNSVCGVNLNASNLYKAGVDDYRATVGPKIDGDWVDAIRAIAAMRNSHLGI
                     AGYRAHGFFNLSVSDPELYGETGLLLDHYELSDIFSEKVEEKAVSLARQKLLDVFEVS
                     GVTELQVAKVSELAAKLDAFCARNSLDALAIRCWPEFARDYGISPCAAMSLLQSEGRL
                     LACEGDVEGALSLMLHKAVGAETPYLFDFSQVDLEKNFALFWHCGVAPCNLWDGKSCR
                     SLDTYFAGGKGVTADFVLKSGELSIARLDTARGQRRLFLQRARAVPMDKELKGTYMKA
                     VFDRPVKEVLDLIIAKGVAHHASVSYGDYMEPLRIAAGLKGWEVVE"
     gene            16671..18815
                     /locus_tag="PLG14_01145"
     CDS             16671..18815
                     /locus_tag="PLG14_01145"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOX47761.1"
                     /translation="MIGLEAGEGGFVLRFKGRPFLVHSRRRPAVLAGRGEGRYASSRG
                     SFRIRDSRREVEPLRGFAVLESGPERAVLAFSGAKGESLRAVFAVEEGRLALRFEGAA
                     PGTNRLVLRLAAPRGERVYGCGEQYSHFQLRGKRIPLWTEEQGVGRGPNLMKLAADLA
                     AGAGGSRTSTYFPIPAFLCAGGAEAGGPEAGGAEAGGASCVGADSSGAPRLGGRGAGP
                     SQAARSGGRSIYAKTSAYAVFDFRRPSSYSLEFWEVPEVLWLDSGDSLPGLVGRLSLL
                     GGRQPPLPEWAYDGLILGAQGGAAEVARKAGALRAAGAPLAGIWAQDWEGQRITSFGK
                     QLRWNWEADEGLYPGIAAFAARLRAEGLRLLGYVNPFLAVDGRQYAEVSPKGYCVKRK
                     DGSDYLVTITTFPAAMLDLTNPAAFAWIKGVIKENLLGSGLSGWMADFGEYLPADAVL
                     FSGESGETAHSRYPVLWARANREAVLEAGAEKEALYFCRSGHEGSAPWAPSFWAGDQL
                     VDWSRDDGLPSVVPAALSLGLSGVGNWHADLGGYTTVGWARRSPELLARWAELAAFAP
                     VMRSHEGNRPESNAQAWEGGESLRLVARAARLHAALKPYQLELARDYEDRGLPFLRPL
                     FLHYPEERRLGTRAYEFLYGPDLLVAPVLKAGARSRRLVLPEDEWVHLWSGRVHRGGR
                     ARVEAPLGEIPVFFRLRSPRASLFAAIARELG"
     gene            18990..19820
                     /locus_tag="PLG14_01150"
     CDS             18990..19820
                     /locus_tag="PLG14_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOX47762.1"
                     /translation="MTKEELAVLEAKAKEIRCKTIDAIGFLGVGHIGGALSIVELLAL
                     LYFKEMRVDPENPRWPGRDRLVLSKGHAGPALYSALAMKGFFPEEWLHSLNKGGTRLP
                     SHCDRNLTPGIDMTTGSLGQGLSAACGIALANRLEGRDSFTFAIIGDGETNEGQNWEA
                     ALFAAHQKLGRLVAFTDYNKMQIDGRTEQVLDLGKLEEKWAAFGWESARVDGHDIAAI
                     AAAVEAAKARAAKPDAKPTMIVLDTIKGKGAAFCEGSVGSHNMAFDYETAKKAIAELG
                     "
     gene            19839..20780
                     /locus_tag="PLG14_01155"
     CDS             19839..20780
                     /locus_tag="PLG14_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOX47763.1"
                     /translation="MATPAKEMRAVYAETLVELGAKNKDIVLLEADLMKASGTGPFQA
                     AYPERTFDVGVAEANMVGVAAGLSAEGKIPFAATFGCFATRRVFDQFFVSCAYARLNV
                     KLVGTDPGVSAAMNGGTHMPFEDLALMRAVPGIAVLEPSDAASLRGLVAAAAERKGSV
                     YMRLHRKAVPDLYPAGEKFEIGKAKLLREGKHVAIVALGSIMVNEALKAAELLAKEGI
                     EASVVDALSVKPLDAELVLREARKCGAVVTAENHQASGGLGSAVAELLAETGAARLAR
                     IGVKDEFGEVGTQDYLQDRFGLRAANIAEAAKALAKA"
     gene            20790..21263
                     /locus_tag="PLG14_01160"
     CDS             20790..21263
                     /locus_tag="PLG14_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOX47764.1"
                     /translation="MKIAIASDLSGFPLKEAIVEHLRSKEGVEVIDFGIPSADQPKPY
                     FLQAPLVAGAIQRGEAERGILICGTGQGMAIVANKHKGIYACVVDDIFSGERAKIVND
                     ANVITMGGWITAPFVAKEIVDGWLSMAFAQKMEFKRDFLANALKEVKALEEKNFR"
     gene            21277..22512
                     /locus_tag="PLG14_01165"
     CDS             21277..22512
                     /locus_tag="PLG14_01165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX47765.1"
                     /translation="MLSSLLSPSRVLSFEELSPADFAKAAFYLGGAPRKAAVVVDGRA
                     FPERDALVAGLQALCETVVLDRVRPDPLASDVDAMREMIGGGELGLVVGIGGGSVLDS
                     AKALAMLAANGGSLSDYLGPAPSRKIEKKGPPLLLVPTTAGTGSEATKVGVFTSDSGR
                     KYTLGSPLLQADAAILSGSLAAGMPPALAASTGFDALSHALESLWNRNATPVTVQAAV
                     DAAVAVLRGLEPAYDAAVAAAAGKPLPGAKAAALRMLEAASMAGFAFSRTGTAMVHAL
                     SFVLSEEWHVPHGAACAFTLEDAYRYALSEPENRARLARIGEALFPEAAARSDAKGGA
                     TESAADRDERLAGLLLERVLVLKRKMGLPERFADLGISVDRGAIPGLFARSFDDPKMA
                     NNFPAAERPAVYAILEAKA"
     gene            22509..23336
                     /locus_tag="PLG14_01170"
     CDS             22509..23336
                     /locus_tag="PLG14_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOX47766.1"
                     /translation="MSLGALLLSGLVVLATHALEAITGFGCTVLALPFVTAILGLKTG
                     VMVLTILAWILALYIAVTKRRDIDFKQYGIILGFVLLGLPLGMYLFRTVEAGSLKRVL
                     AVFIVAASALQLAKPALARGAERRAAQAGPSPAAGSGGEVRGEGEACAAALVPGAPRW
                     LYYLLLVAGGVVHGLFSSGGPFVVLYAAKALPDKGKFRATLCLLWTTLNTVIIASYFV
                     AGTGIGAGAAKATAAMLPFLALGILVGERLHKKATPAVFSTLVFAMLLATGVVMLFF"
     gene            23345..>23792
                     /locus_tag="PLG14_01175"
     CDS             23345..>23792
                     /locus_tag="PLG14_01175"
                     /inference="COORDINATES: protein motif:HMM:NF027487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HOX47767.1"
                     /translation="MDRAGLAARNPHLSVLGPGDPGLAAYGRVLDLPGLEGLVGLADR
                     IVPAGLEANAYVASEAELESHPAAAAFGPAFGFAELQVGWNAGPNSRLNGLEWHKSPE
                     ILVAVSDLALLLGRFEDIELRGPSGPRFDSSRLSCVFLPRGAALELK"
BASE COUNT         3289 a         9023 c         7928 g         3452 t
ORIGIN      
        1 gaagccgtcg cgcaggccgc gcaggtcatg ccgcgtatcg caagtcgtgt cgtcttgatc
       61 atgtgctcct ctctcgaggc ccttccgggc cggttctcat ccgatgtact tctcgaggat
      121 gaggccgatc tcctcgatct tctcggcgcc cttgtcctcc acgaaggcct ccttcacgca
      181 ggtgttcatg tgctgcttca ggatgacgat gttggccttc ttgaggaggg cgacggcggc
      241 gaggatctgc ttggagacgt cgatgcagta gcggtcgtcc tcgatcatgc gcaggacggc
      301 gtctacctgg cccctggccg tcttgagggc cgcgacggcc tctccgctct cgtgactgtg
      361 gtggtccatg ggcccttcct tgtccgatcg attcggtcgc cagttccgac cccaccccac
      421 ccggggaggg ggtatcgact atcaatctac taaggtacgg agcgaaggtc aagcgggagg
      481 gcagggccgg tctccggatc cgccttcccc ccggccgccg cgaggccgca gcaggtcggc
      541 aggcccgcct cggcgaggcc agaaggcgag ccgccgacgc cggagggaag ggccgaggcg
      601 gcgagggggc ctccgaagcc ggcgaggcgg gccgaggggg catcggccgt gggcgcgtcc
      661 gcgaggctcg cgacgacctc gatccggctc ctgatcatgc ccatccagca gctgtagggc
      721 atggcgcccg gggctcccgg cgtgaactcc acgaggttgt cccccgggac cagggtcttc
      781 ttgagccgga gcgccggcac gacgaagggg ttgttgcagc cgttcaggtc ttcggcccgg
      841 acgcggatcg tccatttgag ggggacgccc gcctggacga cgaaggcctg gtagcggttg
      901 gctccgaatt ccgtggtgat gctttggact cccccgtcca gcctggcccg cggaagcgcg
      961 gcgagcgcgg cggccgaggg cgcggcggta aggcggggct ggacgcccgc cgccaggagg
     1021 ggagcggctc cttcccggcc tgccgtggcg ccgcctcggt ccccgccccg ggggcgggac
     1081 aaggtagcgg aggcggggtc cctgcccgcc agggcgaggc ccgggagggc gatgcccgcg
     1141 agggcggccg ccctaccgaa ggtgacgaag gcgaggaaga tcacgaggac ggcgctcgtc
     1201 ttcctcatga gggagagccg cctgagcggc atgagggcgg ctgccaggcc gaagccgagg
     1261 aggaggggcg ccgtgccgag ggcgaagacg aacatggcgg aggcgcccgg gaggaagccg
     1321 cccgtgccca gggcgtagag ctgcatggtc tggagggggc cgcagggcat gaggccgcca
     1381 aggaggccga ccgcgaaggg gccgcgcttt ctcagggccg cggcggcccg ggcggcggcc
     1441 cggcctgccg cctttggcag gaatcttccg gggcgtgagg ggcgcgggaa gaggccgagg
     1501 ctcgagaggc cgaaggccag catgaggatg gccgcgagtc cggtgacggc gcccttggcc
     1561 gcgggcgaga agtcgagggc cgagccgagg gcccccgcga ggccgcccag gagcgtgtag
     1621 gagaggatcc gccccccgtt gtagaggagg cccgggccga gccgctcgag ccggccgggg
     1681 gccgcgccag tccgggcctc gccgccgctc tcccgggcgc cggccgagcc gaggctctgg
     1741 gaaagggcta tgccgccgca catggcgacg cagtgcgcgg aggtgaggag gccgacgacg
     1801 aagagggctc cgtagccgag gctcgagtcg atccggggca ggttgttgaa gaggccggcg
     1861 gcgcccgccg ccaggtaggc gcccgagagg agcagcccga gggcgagggc gggcaggagc
     1921 tccgccgccc gcgccgccag cccggagggc aggaccgagt agcccgctcc ctcgaccgca
     1981 gcttccagga cggcaaggtc ctccaggccg tgctccaccg tcgcctcgcc ccgctcgagg
     2041 ctcacctcga cggactcgac gccctgcagt ccggccaggg ccgcctgaat cctcgcctgg
     2101 caggaaggac aagtcatccc tccgattctg agtttcgtcc gcttgattgt catgcttcgc
     2161 ctcgctccgc ggtagttctc gcgagggagg atatccgggc ggtgtgaagg attgatgaac
     2221 ggcaggcgcg gttttcgtgc ctatttttcg tgccgaagcg tcgcctcagt cccgcggcgg
     2281 cctactcgac ggcgaggatc tcgaggcggc gctcgcctgc cgggaggcgg acgagggcga
     2341 cctcgcccgg ccgcttcccc atgagggccc gggccacggg ggaggtccag ccgatgaggc
     2401 cgcgctcggg ctcggcttcg tcgacgccga cgatgcggta ttccgactcg gccccggcgg
     2461 cgccaggccg gccccgcggc ccttccccgg cctccgcggc gccgccgccc ggcgcctcgc
     2521 ggacgcggat tcgcgcgccg aacttcacgc ggtcgtagcc gccctcgggg ggagggatga
     2581 cctcggcgag ggaggacatc atggagaggt actcgacgcg ccgctcgacg aggccgagcg
     2641 agcggcgcag ggcggcgagc tcgtccttgt cggcgccggc catccgcgcg acgtcggcta
     2701 ggatgcgggg cctcgtctcc tggacgagct cgtggagctc ccgggagagc cgctctgcgc
     2761 cttcgggggt gaggtagttc cgcgagccct gcgggatggg gatcttgatc tccggagcct
     2821 cgctctctcc cgccgtcgaa tcgtcctcgt ccacgaaggc cctgctcatc ccgcctcctc
     2881 gcccttgcgc ggccccgcgg ggccgtgaaa ccgttctaca cccggtgccc atcgggcgca
     2941 aggatgctag actgtcgccc gcaagaccca ggaggcagcg catgaccgag cccttcgtgt
     3001 tccgttcccc gacccgactc gtattcgggg ccggctccgt ggaatcggcc ggcgccgagg
     3061 ccctggccgc ggtgtcggct ttccaggcgg ccgccgcttc gtcttccgcc gcctcgcctg
     3121 gcggcgccgg cctaccgggc ggcggctccc cgcgcggcgc ggcggagagg cgccgcctcg
     3181 ccttcctcgt cacggggaag ggccacaccg cccgctcggc gggccttgcc cggctccgcg
     3241 cctccctgga gcgctcgggc ttcgccgtcg agctctacgc ggaggcgacc gccgatcctc
     3301 ccgtggagct cgtcctcgcc tgcgccgagg gcctggccgc ctccggcgcc tccctggtcg
     3361 tcgcctacgg cggcgggagc cccatcgact gcgcgaaggc ggcctcggtc tacgccgcca
     3421 acacggcgcc gcaaggtccc tttccgggcg cgcccttcct cgacttcctc tacggccgcc
     3481 tcgtcttctc ggcgccgggc ctccccctca tggccgtccc gacgacggcg ggctcgggga
     3541 gcgagatgag cgccgccgcg gtgacgaccg acctggcggc ctcctccaag cggggcctct
     3601 cgagcgactt cttcttcccc cgcgctgcgc tggtggaccc cgagctccag gcgacgatgc
     3661 ctcccgccct ggcggcggcg accggcatgg acgccctgac ccacgccatc gagtccttcg
     3721 cctccctcaa ggcgacgccc ctcacgcggg ccatcgccgg cgagtccgcg cgcctcatcg
     3781 ccgggagcct tgagcgggcg gtggcggccg cggccgaggg ccctggcggc gcggcggacc
     3841 tcggcggcgc cgaagcggcc ggcgcggcgg cgataggcgg cgcaggcggc gcggccgcga
     3901 gggccgcggc ggcggcccgg gcggacatgg ccctggcgag cgcccaggtg gctatggcct
     3961 tcagccagac cggactgggc atggtccacg gtttcgccca tcccgtcggc gcccgggccg
     4021 gcgtcgccca cggcgtcgcc aacgccgtcc tcctgcccta cgtgatggag gcctgcgccg
     4081 aggcggcgcc cgggcccttc gccctcctgg gcgacctttt cgccgcggct tcgccctcgg
     4141 gcgaggccga ggtcagggcc gcgggggagg ggagcctcgg cgcccggcgc ggcggccttg
     4201 cctccggcct acgcgcgccc gaccggcgcg cgcccgaggc cgaacgcgcg gcctacgccg
     4261 tcggcttcgt ccgggagctc tcccgcgcca tcggcattcc cgcgcgtctc cgggagctcg
     4321 gcgtcgacag gggcgatctt cccgccatcc tggccgacgc catctcctac cgcggccgcg
     4381 gcggcagccc ccgcgccttc acggacgccg agctcggcgg gctcctggag cgggcttact
     4441 gagaagcggg cccgcgcatc cgccaggccg gccctcgcgc caaagccaag ccggggccgc
     4501 gcgcaagcca gcttgagtcc gcgcgccagc caggccggcc cgcgcggccc gcttgccgcc
     4561 tacgcgccgc ccgcgaggcc cgcgagggcg gcgagcatgg cgcggcccgc gagggctccg
     4621 gtggagaagg cggcctggag gttgtagccg cccgagtcgc cgtcgaagtc caggacctcg
     4681 ccggcgaaga agagccccgg cgcgacgcgg gattccatgg tcttgggcga gacctcggcg
     4741 aggtcgacgc cgcccgcggt cgccatcgcc tcgtcccagc ccgagaggct cccgacctgg
     4801 acgggacatt cgcaggcgag gcggcagagc gcctcccggg actctcggcg gaggtcggcg
     4861 gccgccgtct ccggggccag gcccgcgagg gagcagaaga gctcggccat ggccttgggc
     4921 aggccggcct ccgcgaggac ggtgcgcgcc agccggcgcg gctccttcgc gaggaggcgg
     4981 tcgaatcgct cccggaattc ctcgaggcct acgtcggcga agcggagctc cagcgtgtcg
     5041 ccgggctcca tgccccgcga ggagtccagc acgaggggcc cggagaggcc ctcgtgggtg
     5101 atgaggaggt cgccctcgcc ggcgaggacg cgcttccctc cgcgcctgag cgcgaggcca
     5161 gcgccgcgga aggagaggcc cgccaggggc tcgaggcgga agcccttcgc gcggacggcc
     5221 gccagggcgg gccgcggcgc gacgaccgag tgtcccaggg ccttcgcgag ggcgtagccg
     5281 tcgcccgtgc tgcccgtggc cgggtaggag gctcctcccg tcgcgaggag gaggaggggg
     5341 gcggcgtagc tctcctcggc gccggacccc tcaggcccgg tcctcgccgc gcggaggagg
     5401 aagccgcccg ccgccacggc cctttcaggg ccgcgcatcg cgccggccgc gtccgcggcc
     5461 gcgcggccct cgccgtcagc tccgggcccg ccgctttggc cctccccgac ccgctcggcc
     5521 gagaggaggc ggcggcccgt cgcgacgacg atcccgaggc gggagcattc ggaaaggagg
     5581 aggtcgagga cggaggaggc ccgcctcgtc gcggggaaga ccttgccgcc cgcctcggcc
     5641 tcgaagccga ggccccgggc gcggaaccag tcgaggaggt cctcgttcgc gagggcgtag
     5701 agggcgggct tgaggaagcg ccccgcggcg ccgggcttcc cgccgccgcc gtagcggggc
     5761 aggaagtcct ccatcgggcc tgcgtgggtg aggttgcact ggcccgagcc cgagaggagg
     5821 agcttcctcg ccggccgctc gcgccgctcc aggacgagga cgcggggcgg gggcccggcg
     5881 ggcgccgcgc cggtcccgcc gtcgccggcc gcgccgtcgc cgcgggggtg cgcctccgcg
     5941 ggcgcggccg ccattgcggc gcccgttccg gcgtcgattc gggccctccg cgcgcggagg
     6001 ccttcggcga gggtgatggc cgcgaagagg ccggcggggc cgccgcctgc gacgacggcc
     6061 gcgaatcgct caggtacgct catcggcccg atggtagtcc ccggaaggcc ggagggtcaa
     6121 cgagagaagg aacttgacgc ccggcgtccg gcgggctagt ttggtgccgc tccgtcgccg
     6181 tctggacgga cgagtccgaa gtgaggagtc agcatggaca gcgtcgcgcc aaaggcggtc
     6241 cgccgccgct tcggggaccg ctacgacggg aggcgcctcc gctccttgag ccccttctac
     6301 cagatcacgc cctacatcat gcgacgccgc tccgacgccc aggactattt cgaggaccgc
     6361 atcgagatcg gcgcggccga ggcctggctc aggcggctcc gcgacggggg gcggccggac
     6421 atcggcttcc tccacctctt catcgccgtc ctcgtccgga ccatggccct gcggcctcgc
     6481 ctcaaccgct tcgtggccgg ccagcgcatc tacgcccgga acgagatcct cgtcagcctg
     6541 gccctcaaga agcggctcca tgaggacagc ccggagacga cggtgaagat ggccttcctg
     6601 ccgacggaca ccatcttcga cgtggcggac aaggtcaacg ccgccgtgga ggcgaacaag
     6661 gcagcggaat cctcgaacca gacggacagg gcggcgaggc tcttcatgct ctgtcccggc
     6721 ttccttgtcc gcttcctggt ctggggtctt cgctgcctcg actacgccgg cctccttccc
     6781 aaggccatcc accgggcgag ccccttccac gcgagcgcct tcgtcaccga ccttggttcc
     6841 ctggggatcc ggcccatcta ccaccatctt tacgatttcg gtaccacctc tatcttcgtc
     6901 gccttcggct cgaaggagcg cggccgggaa ctcgacgagg agggcctgcc ccgggccagg
     6961 aagcacatct cggtgaagat cgtcaacgac gagcggatct gcgacgggca ctactacgcc
     7021 agcgccttca agtacatgct cggcctcttc aggcggccgg aggagctcga gacgcggccg
     7081 ccctcggtcg ccagggacgt cgattgatgc tatagtcggg cccatggccc gaagcttctt
     7141 ctggtacgac ctcgagacct tcggcctcga gccgagatac gaccgcatcg cccagttcgc
     7201 cgggctccgc accgacgaga acctggagga gacgggcgag cggatcatcc tctactgcaa
     7261 gccgacgccc gactacctgc cgagccccca ggcctgcctg gtccacggga tcacgcccca
     7321 ggccgccatg gaggagggcc tctccgacta cggcctggcc ctgcgcctcc gccgcgagct
     7381 catggagccg gggacgacgg ccctcggcta caacaccctc cagttcgacg acgagttcct
     7441 gcggagcctc ttctaccgca acttcttcga tccctacgag cgggagtgga ggaacggcaa
     7501 cggccgctgg gacctcgtgg acctcatgcg cgcgatgcgc gacctgcggc ccgggggact
     7561 cgtctggcct acgggggagg gcgggcggcc catcttcacg ctggaggccc tggccaaggc
     7621 gaacggcgtc gcgcacaagt ccgcgcacga cgcgatgcac gacgtcctcg ccaccctggg
     7681 cctcgcgcgc ctcgcgaagg cgaggcagcc gcggctcttc gactggtacc tctcgcaccg
     7741 gacccgcgac tccctgcggc ccctcatcga cctcgtggag cggagccccc tcgtccacac
     7801 ggcagccgcc tacacctcgg agcggggctg cacgaccctg gtcgcgccca tctccctcga
     7861 tccggagaac cgcaaccagc tcctcgccat cgacctccgc tacgaccccg cggagatcct
     7921 ggacctgccc gtggaggagc tcaggcgccg cgtcttcacg aaggccgagg agcttgaggt
     7981 gccccgcctc cccctggccc gcctccgcct gaaccgctgc cccttcctgg ccccccgggc
     8041 ggccctctcg cccgaggccg ccgagcggct gggcatcgac gcggccgcct gcgaggccca
     8101 cctctccctc atcaagaagg agcccgagct catccaaaag ctttccgccg tcttcgaggg
     8161 gcccttgccc ccttccgagg agagcgaccc cgagctcagg atctactcgg acggcttttt
     8221 ccccgaggag gacgccgaag tcttcgcggc cctccacgag gccctggccg aggccttggc
     8281 cggcgccgag ggcgcggccg gagcgggcct cgcggcgagc ggcgcggccc cggcaggcgc
     8341 ggcggccgcg gaggggagcg gcgcggctcc gggcggcgag ccggccgcgg gcaccgcttc
     8401 cgccctcctt gagaccaagg cccgcttcta ccgccgccgc ttccgcgacg acaggccgcc
     8461 ccagatgctc aggcgcttct tcgcgcgcaa cttccccgag accctcggcc ccgaggaggc
     8521 gaggcgctgg cgcggcttct gcgctagcag gctccagctt ccccccgtcg agggggtcgc
     8581 cgacctcgcg actttctcga aattcgtcgg ccagaggctg gagacgccga gccttccctc
     8641 ccgcgacaag gccatccttc tcgccctcct cgactaccgg agcaggctcg agagggaggt
     8701 cctggactat cccggagcct gaagaaggcc atcgttttcg cttgacagga cgcaattcct
     8761 ctcctaccat ggattaactt actttgtgaa agtaagtata ggaggcagaa tgaaggcaag
     8821 tatccgccgc acgctcgccg tagcggccat cgctctctgc gcgctacccg ccatgctcgc
     8881 ggcccagtcc ggcgagatcg tcctcaagtg gccctgcatc tgggtcggca aggactcaaa
     8941 ggccccggcg atcgcggaga tcgtcgactc cttcaacaag gccaacgccg gcaagatccg
     9001 ggtcgaggtc gagccccagc ccgactacaa cgcctacgag cagaaggtcc gcacctccct
     9061 ggccgcgggc gtcgcgcccg gcgacatctt caccctgaag ctcaacgcca ccacgaagga
     9121 attctacggc tccaagtaca tcatggacct cacgaaggac ttctccggct cctggaaggc
     9181 ctccttcgac tcgggcgccg tggcccagtc cacgatcgac ggcaagctca agaccctgcc
     9241 cttcgagacc gccgtcctcc ccatctggta caacatggac gcgatgagga aggccggcgt
     9301 ctccgccccg cccgcgacca tgcccgagct ctggaccgcc ctggacaagc tcaaggcctc
     9361 cgggatctac ccgatgagcc agatgaccgg cgacacgaac gcctggacga gcatgatctg
     9421 gttctcgcac ttcgcggtct ccttcggcgg agccaaggtc tgggagaagc ccttcaccga
     9481 caaggccttc gtcgagtccg ccaagctcat gcagaggatc ttcaaggact acacgacccc
     9541 ggacgccgtc ggcgccggcg ccggcgtctc cggcggccac ttcctcgcgg gccgcaccgc
     9601 catcttctcc aacggcccct ggtacgccgg ccgcgccgac ctccgggcca ccgccttctt
     9661 cccaagcgtc accatcgccg ccgccccgcc cgccggctcc accaagggcg tgatgatcag
     9721 ccgcctccag gccaacgtcg ccgccggcga gacgaaggac aagaaccgcc gcgcggcgat
     9781 cgtctccttc ctcaagtacc tcaccgcccc cgagaacatc tccaagctcg cccagggctc
     9841 cggcgccatg ttcgcggtga agtccggcta cgtgcccacc gacgccctcc agaagcagtt
     9901 ctacgacatc gccgccgcct ccaagacgac ggccaacgac ctcgaggccg ccctcggctc
     9961 cgaggccacc ctcgagttcg cccagcagct gggcgccctc gtcctcggca acatcacgcc
    10021 cgagcagttc tgcgccctcg tcgagaagaa gatcgaccgc tagacattcc tccggtcccg
    10081 ggttccctcc gggccgcctg gcgcggccac ggagggggag gcccggggcc tccatgaagg
    10141 acaacgatga agaagcgccg caatcccaac gccctcaaga tcgccctgtt cctcgcgccg
    10201 gtcctggtcc tgttcgcggc gttcttcgcc taccccttcg tcttcaccat cgccgcgagc
    10261 ctcacgaagt ggaggggcat cggcgcgatg cgcttcatcg gcttcggcaa cttcgggaag
    10321 ctcctgggcg accagacctt ccgcctcgcg atcaggaaca acgtcgtctg ggccctcgcc
    10381 tcgggcttcg tccaggtgcc cctggccgtc ctcgtggccc tcatcctcgc gcgggagccc
    10441 cgcggctgga aggtcctgcg caccgtgtac ttcctgccca acgtcatctc gaccgtcgcc
    10501 ctggcgatgg tctggacggc gatgtacaac ccccagtacg gcgccctgaa cgggctccgg
    10561 gagcttctcg gcctcgaggc caggaactgg ctcggcgacc ccgcgacggc cctgcccgcc
    10621 gtcatcctcc agggcgtcct ctacatcggc tacttcatga tcatcatcct ggcggcggcg
    10681 atgaacatcc cgcgcgagct ctacgaggcg gccgagatcg acggcgccac taccctccag
    10741 caggaattcc gcataacgct gccgatgctc cgcgggagca tcgtcacggc catgactctg
    10801 gcgatggcct acggcatgcg ccacttcgag gccacctacc tcatgacggg cggaggcccc
    10861 gcctactcca cgacgacgat gggcatcgac ctcttcctca agatggacgc cctgcgctac
    10921 agcgaggcga gcgcggccgg cgtcatcctc atcgtcctcg ggacggtggt gatcgtcgcg
    10981 atccgccgcc tcctggggcg gaccgacccc atggccgagg ccggccagta agcacggagg
    11041 agacgaacga tgcgcgctaa gaacaaggcc ctctccctgg gcgcgggcct cctcaagtgg
    11101 acggtcctcc tcttcttcct ggcggtggcc ctcctgcccc tcgcctggct cgtcctcaac
    11161 tccttcaaga cgaacctgga gctccagtcc tcgcccttct ccctgcccgc gaggccggcc
    11221 ttcgagaact acgccaacgc gatcgcgatg gccgagctcc cccgcctctt cgtgaactcc
    11281 gtcctggtcg tcgtggggac cgtcttcctc aatctcctcg tctcctcgat ggccggcttc
    11341 gtcctggccc gggaggcctg gaagggcagg gacacggtcc gcacggtact ggaggccggc
    11401 gtcctcgtgc cgatcatcgc cttcatggtg ccctacttca ccctcgtgac gaagctcggc
    11461 ctctacgaca agctctacac gctgatcctc gtctacgcgg cgatcaacat cccggtgtcg
    11521 atcttcctcg tgacctcctt catgcgctcc atccccaagg agctcgagga ggcggccatc
    11581 atcgacggct gcggcttctt ccagcgctac tcgaaggtca tcctgcccct gtcgcgctcg
    11641 ggcctcgtga ccgccggcac cttctgcttc atctacgcct ggaacgagtt cgtgatggcc
    11701 atgctcctca cctccagcat ccactcgcgg accatccagc tcggcatccg cttcttcacg
    11761 agccagttca tcaccgacta cgccgggatg ttcgcggcca tcgtgctctc catgctgccg
    11821 agcgtcctcg cctacgtctt cctgcacaac aagatcatcg ccggcctcac cgcgggcgcg
    11881 gtcaagggct gacgcgatgt ccgacctgga gatcgtccgg atccccggag gcttcgaggc
    11941 ccgcctccgc ggacgcctcg tcctggccca ccgccccggc gagcccctcc tccgcgcggg
    12001 ccgcggcgag gcccgcttcg agatgtaccg cggcaacttc gaggtctccg agcgcctgga
    12061 cgagctcgtc gccctggagg actgcggcct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn cccctctgga cgagcgagca gggcgtgggc cgcaacaaga ccaccgaggt
    12241 cacgaggctc gccgacctcc acgacaaggc gggaggcgac tactggtgga ccttcttccc
    12301 cgagccgacc ttcgcgacga gccgcaggct ctcctaccac ctggagacga gcgcctacgc
    12361 cgcattcgac ttccgctcgc ccgggcgcca cgagctctac gcctgggccc tgcccgggcg
    12421 cctggtcata ggcgcggccg atacgatgcg cgggctcgtc tccggcgtct ccgccctcct
    12481 cggccgccag cccgagctcc ccgactgggc ctacgacggc gtggtcctgg ggatccaggg
    12541 cggcacggag gcctgcctct ccaagctgga gcgggcccgg aaggccggcg tccccgtcgc
    12601 gggcatctgg gcccaggact gggaaggcat caacatgacg agcttcggcc agcgcctccg
    12661 ctgggactgg caatgggaca gggagcgcta ccctggcctt cccgccgaga tcgcgcgcct
    12721 ccgggccgag ggcgtccgct tcctcggcta cgtgaatccc tatgtcgccg cggggaagcc
    12781 cctgtacgcc gaggcgtcgg cccagggcct cctcgcgcga cgcgccgacg gctcggagta
    12841 cctcgtcgac ttcggcgagt tcgacgccgg catagtggac ttcacgaacc ccgccgccgt
    12901 ggagtggttc aagggcgtca tcaagaagaa cctcgtcggc atcggcctct cgggctggat
    12961 ggccgacttc ggcgagtacc ttcccacgga cgccgtcctc ttcgacggga cgccggctga
    13021 gatagcgcac aacgcctggc ccgccgtctg ggcccgcgtc aaccgcgagg ccgtggacga
    13081 ggcggccgca gaggccgccg cggccaggac cgccgccgcg ccaggcgcgc cgccggtcgc
    13141 cggcgccagg ccgcccgcct cgggcgccgc cgccgcttcg gccgcctcag ccggcggcgc
    13201 cgccgccgct ccctccgccg cgcccgcctt cgacccggcc gagtgcctct acttcatgcg
    13261 ggcgggcttc acgggcagcc aacgcgactg tcccctcatg tgggccggcg accagaacgt
    13321 cgactggtcc aaggacgacg gcctcccctc agtgataccc gcggccctct ccctcgcggt
    13381 ctgcggccac ggcctccacc actcggacat cggcggctac acgaccctct tcggcatgag
    13441 gcgcacgaag gagttattcc tccgctgggc ggagcaggcc gccttcaccc ccttcatgcg
    13501 gagccacgag gggaacaggc ccaaggacaa ctggcagttc gactccgacg aggagaccct
    13561 cgcgggcctc gggcgcatgg gccggctcca cgccgcgctc aagccctacc tcaaggcctg
    13621 cgtgggggag aacgcccgcg agggcgtccc cgtgatgcgg cccctcttcc tccacttcga
    13681 ggaggacccc gaggcctgga gcctcgagga cgagtacctc ctcggccccg acctcctcgc
    13741 ggcgcccgtc ctggaggagg gcgcggcgag gcgccgcctc cacctgccgc caggcgcctg
    13801 gatccacctc tggtccggaa gccgcctaga ggcgccggcc cccggcggcg cgtccgtcga
    13861 ggtcgaggcg cccttgggcg agccgccggc cttctaccgc gaaggctcgc tctggaccgc
    13921 gaccttcgcc gcggcggcgg ccgcggcccg cgccgcgcgg ggctccgccc cgggacgatg
    13981 agggaccccg cggccgcgca ttgcgcggca gggccgcgat gaactactat cctgggaata
    14041 tgtcgcccaa ggacaacccc ctccgcgccg ccgacgtgcg cgagcgcaac gagaagatcg
    14101 tgctccgcct catccgcggc gcgggcgcga ggggcctctc gcagtcggag gccgtcgtcg
    14161 cgacgggcct ccgcgccccg accatcttcc gcatcttctc cagcctggag gaggagggct
    14221 acgtcgagcc cctggctccg gcctccgccg aggaggaggg gagggagagg aagggccgcc
    14281 cccgcgtctc cttcgtcgcc agggccgacg cgctctacgc catcggcgcc gagttctggg
    14341 tggaccgcgt ctccctgggc gtcttcgact tccgcggcga gcgcgtcgcc tcctccgtcg
    14401 cctccctgga caagggcgtg gacgcggagg ccgtggtcga ggccatagcc cgctgcgccg
    14461 agggcgcgat cgagtcgctc ggcctctcaa aggaccgcgt cctgggcctg ggcctgggcg
    14521 cgcccggcca ggtcaacgtg cgcgcgcggg agatagcctt ctactcccgc atcccaggcc
    14581 tccgcgactt ccccatggcc gcgcgcctgg aggatcggct cggcctcccc gtctccatcc
    14641 acaacaactg ctcgatcatc gccctctcgg agttccgcta cggcggcctc caggcggccg
    14701 actccctctt catcttcctc ctccgctcgg gcgtcaacgg cgccttcgtg gacgggggcc
    14761 gcgtctacct cacctccgac ggcacgacga tcgagtccgg ccacatctcc gtggactacg
    14821 acggcgagcc ctgcgcctgc ggcgcccgcg gctgcctgga gacctacgtg accgccctgg
    14881 acgggcccaa cctggagtcg ggcaagtggc tcttcgaggg actgggcccc gccctggagg
    14941 cgggcgaccc gaaggccgag gcgaccctcc gcgaggcggc cctctacatc gcctccgcgg
    15001 cgcggaccgt ttcgcggctc ttcaggccgc gggccttcct catcgtcgcc gtctcggact
    15061 ccgtctccga agccctggcc cgcctcgtgc gggaacggct cgagagctcg gcctccggct
    15121 tcgaccgccc cgcgcccctc ttcgaggccc gcgcctacga ccctggcctg gcccagcgcg
    15181 gcgccgccga cctcgtcctg gacgacttcc tgggctagcc gcccgtcgcg ggggcgcggc
    15241 ggcgcgccaa tgagcctggg cggcgccgcc tccccctcga cactatccgc ttgacggctc
    15301 cttgaacttg gtatttactt acaaaaagaa agtaaatcct cgaccaagga gccccgcatg
    15361 aaacagcata taaagctcgg cctcgtctgc ctggcgcgcg agaccttcga cttcaacgcc
    15421 gccgccgaga tctacggccg gatccggggc gagctggcct ccctggagaa cgtgagctgg
    15481 gagatggtga gcgaactcgt gatctccgtg gaggacgcga agaaggccgc cgcccgcctc
    15541 gcctccgcct ccctggacgc cctggtctgc atctccggca ccttccacct cggccacctc
    15601 gtcctcgaga tcaggaagcg gaaccccctc cccatcctcc tttggggcct cgacgagctc
    15661 ccctacaacg gcgggaagat caggctcaac tccgtctgcg gagtgaacct caacgcctcc
    15721 aacctctaca aggccggcgt ggacgactac cgcgcgaccg tcggcccgaa gatcgacggg
    15781 gactgggtgg acgcgatacg cgccatagcc gcgatgcgga actcccacct cggcatcgcc
    15841 ggctaccgcg cccacggctt cttcaatctc tccgtctccg accccgagct ctacggcgag
    15901 acgggcctcc tcctcgacca ctacgagctc tccgacatct tctccgagaa ggtcgaggag
    15961 aaggccgtgt ccctcgccag gcagaagctc ctcgacgtct tcgaggtctc cggcgtcacc
    16021 gagctccagg tggccaaggt ctccgagctc gcggccaagc tcgacgcctt ctgcgcgcgc
    16081 aactccctgg acgccctggc tatccgctgc tggcccgagt tcgcccgcga ctacggcatc
    16141 tcgccctgcg ccgcgatgtc cctcctccag tcggagggcc gccttctcgc ctgcgagggc
    16201 gacgtcgagg gcgccctctc cctcatgctc cacaaggcgg tcggcgcgga gacgccctac
    16261 ctcttcgact tctcgcaggt cgacctggag aagaacttcg ccctcttctg gcactgcggc
    16321 gtcgcgccct gcaacctctg ggacggcaag tcctgccgct ccctggacac ctacttcgcg
    16381 ggcgggaagg gcgtcaccgc cgacttcgtc ctcaagtccg gcgagctctc catcgcgagg
    16441 ctcgacaccg cccgcggcca gcgccgcctc ttcctccagc gcgcccgcgc ggtgccgatg
    16501 gacaaggagc tcaaggggac ctacatgaag gccgtcttcg accggcccgt gaaggaggtc
    16561 ctggacctca tcatcgcgaa gggcgtcgcg caccacgcct ccgtctccta cggcgactac
    16621 atggagcctc tccgcatcgc ggccgggctc aagggctggg aagtcgtcga gtgatcgggc
    16681 tcgaggcggg ggagggcggc ttcgtcctcc gcttcaaggg ccggcccttc ctcgtccatt
    16741 cgaggcggag gccggccgtc ctcgccggcc gcggcgaggg gcgctacgcc tcctcccgcg
    16801 gcagcttcag gatcagggac tcgcgccggg aggtcgagcc gctgcgcggc ttcgccgtcc
    16861 tggagtcggg ccccgagcgc gccgtcctgg ccttcagcgg cgccaagggc gagtccctca
    16921 gggccgtctt cgccgtggag gaggggaggc tcgccctccg cttcgagggc gcggcgccag
    16981 ggacgaaccg cctcgtcctc cgcctggcgg cgccgagggg ggagcgggtc tacggctgcg
    17041 gcgagcagta cagccacttc cagctgcgcg ggaagcgcat ccccctctgg accgaggagc
    17101 agggcgtcgg ccgcggcccg aacctcatga agctcgccgc cgacctcgcc gccggcgccg
    17161 gcggcagccg cacctccact tactttccta ttcccgcctt cctctgcgcg ggcggcgccg
    17221 aggccggcgg ccccgaggcc ggcggcgccg aggccggcgg cgcttcgtgc gtaggcgcgg
    17281 acagcagcgg cgccccgcgc ttgggaggcc gcggagccgg cccgagccag gccgcgcggt
    17341 ccggcggccg ctccatctac gcgaagacct cggcctacgc cgtcttcgac ttccgccgcc
    17401 cgtcctccta ctccctcgag ttctgggagg tccccgaggt cctctggctc gactcgggcg
    17461 acagcctacc cggtctcgtc ggccgcctct ccctcctcgg cgggcgccag ccgcccctcc
    17521 ccgagtgggc ctacgacggc ctcatcctgg gagcccaggg cggcgcggcc gaggtcgcgc
    17581 gcaaggccgg ggccctgcgc gccgccggcg cgcccctcgc ggggatctgg gcccaggact
    17641 gggagggcca aaggatcacg agcttcggga agcagctacg gtggaactgg gaggccgacg
    17701 agggcctcta tcccggcatc gccgccttcg ccgcgcggct ccgggccgag ggcctccggc
    17761 tcctcggcta cgtgaatccc ttcctcgcgg tcgacggcag gcagtacgcc gaggtctctc
    17821 ccaagggcta ctgcgtcaag cggaaggacg gctccgacta cctcgtcacg atcacgacct
    17881 tccccgccgc gatgctcgac ctcacgaatc ccgcggcctt cgcctggatc aagggcgtca
    17941 tcaaggagaa cctcctcggc tccggcctct cgggctggat ggccgacttc ggcgagtacc
    18001 tccccgccga cgccgtcctc ttctccggcg agtcggggga gacggcccac tcccgctacc
    18061 cggtcctctg ggcccgcgcg aaccgcgagg ccgtcctgga ggcaggcgcc gagaaagaag
    18121 ccctctactt ctgccgctcg ggccacgagg gctccgcgcc ttgggcgccg tcgttctggg
    18181 cgggcgacca gctcgtcgac tggagccggg acgacggcct tccgagcgtc gtgccagcgg
    18241 ccctctccct gggcctctcg ggcgtcggga actggcacgc cgacctgggg ggctacacga
    18301 cggtcggctg ggccaggcgg tcgccggagc tcctcgcgcg ctgggctgag ctcgccgcct
    18361 tcgcccccgt catgcgcagc cacgagggca acaggccgga gtcgaacgcc caggcctggg
    18421 agggcgggga gagcctccgc ctggtggccc gcgccgcgag gctccacgcc gccctgaagc
    18481 cctaccagct cgagctcgcg cgcgactacg aggatcgggg cctgcccttc ctgcggcccc
    18541 tcttcctcca ctatcccgag gagcgccgcc tcgggacgcg ggcctacgag ttcctctacg
    18601 gccccgacct cctcgtcgct cccgtcctca aggcaggcgc gcgctcgcgg cgcctcgtcc
    18661 tccccgagga cgagtgggtc catctctgga gcggccgcgt ccaccggggc gggagggcga
    18721 gggtcgaggc gccgctgggc gagattccgg tctttttccg gctccgttcg ccccgcgcca
    18781 gtctcttcgc ggccatcgcc cgcgagctcg gctaggctcc cgcgcccggg cgcggcagta
    18841 tgggtcccgg ctggcgagcc gcgggcgcgg tagtcgtcgg cggctcgggg ggacggagtt
    18901 gcggcgtttt cctgctttac agtccccagc tctgatgata ttgttactta gcgaaagaaa
    18961 gtaaatccta tcaagggaga tacgtgagca tgaccaagga agaactcgcg gtcctggagg
    19021 cgaaagccaa ggagatccgc tgcaagacga tcgacgccat cggattcctg ggcgtcggac
    19081 acatcggcgg agccctctcc atcgtggagc tcctcgccct cctctacttc aaggagatgc
    19141 gcgtcgatcc tgagaatccc cgatggcccg gccgcgaccg cctggtcctc tccaagggcc
    19201 acgccggccc cgccctctac tcggccctgg cgatgaaggg cttcttcccc gaggagtggc
    19261 tccacagcct caacaagggc gggaccaggc tcccgagcca ctgcgaccgc aacctgacgc
    19321 ccggcatcga catgacgacg ggctccctgg gccagggtct ctcggccgcc tgcggcatcg
    19381 ccctcgcgaa ccgcctggag ggccgcgact ccttcacctt cgcgatcatc ggcgacgggg
    19441 agacgaacga ggggcagaac tgggaggcgg cgctcttcgc cgcccaccag aagctcgggc
    19501 gcctcgtcgc cttcaccgac tacaacaaga tgcagatcga cggccggacc gagcaggtgc
    19561 tcgacctcgg aaagctcgag gagaagtggg cggccttcgg ctgggagtcg gccagggtgg
    19621 acggccacga catcgcggcg atcgccgccg ccgtcgaggc cgcgaaggcc cgcgccgcca
    19681 agccggacgc caagcccacg atgatcgtcc tggacaccat caagggcaag ggcgcggcgt
    19741 tctgcgaggg ctccgtcggg agccacaaca tggccttcga ctacgagacc gccaagaagg
    19801 cgatcgccga gctcggctga aggaagcgag gagacagcat ggcaacccca gcgaaagaga
    19861 tgcgcgcggt ctacgcggag accctcgtgg agctcggcgc gaagaacaag gatatcgtcc
    19921 tcctggaggc ggacctcatg aaggcctccg gcaccggccc cttccaggcc gcctatcccg
    19981 agcggacctt cgacgtcggc gtggccgagg cgaacatggt cggcgtcgcc gccggcctct
    20041 ccgcggaggg gaagatcccc ttcgccgcga ccttcggctg cttcgcgacg cgccgcgtct
    20101 tcgaccagtt cttcgtgagc tgcgcctacg cacggctcaa cgtgaagctc gtcggcaccg
    20161 accccggcgt ctccgccgcg atgaacggcg gcacccacat gcccttcgag gacctcgcgc
    20221 tcatgcgcgc ggtgccgggc atcgccgtcc tcgagccctc cgacgcggcc tccctgcgcg
    20281 gcctcgtcgc cgccgccgcc gagcggaagg gttccgtcta catgcgcctg caccgcaagg
    20341 ccgtgcccga cctctatccc gccggcgaga agttcgagat cggcaaggcc aagctcctcc
    20401 gcgaggggaa gcatgtcgcg atcgtcgccc tcggctccat catggtaaac gaggccctca
    20461 aggccgcgga gctcctggcc aaggagggca tcgaggcctc cgtcgtcgac gccctctccg
    20521 tgaagcccct cgacgcggag ctcgtcctcc gcgaggcgcg caagtgcggc gccgtcgtca
    20581 ccgcggagaa ccaccaggcc tccggcggcc tcggctccgc cgtcgcggag ctcctcgccg
    20641 agaccggcgc ggcccgcctc gcgcggatcg gcgtcaagga cgagttcggc gaggtcggca
    20701 cccaggacta cctccaggac cgcttcggcc tcagggccgc caacatagcc gaggccgcga
    20761 aggccctcgc caaggcataa ggagccgaca tgaagatcgc catagcctcc gacctctcgg
    20821 gcttccccct caaggaggcc atcgtcgagc acctccgctc caaggagggc gtcgaggtca
    20881 tcgacttcgg catcccctcc gcggaccagc ccaagcccta cttcctccag gcccccctcg
    20941 tcgccggcgc catccagcgc ggcgaggcgg agcgcggcat cctcatctgc ggcacgggtc
    21001 agggcatggc catcgtcgcg aacaagcaca aggggatcta cgcctgcgtg gtggacgaca
    21061 tcttctccgg cgagcgcgcc aagatcgtga acgacgcgaa cgtgatcacg atgggcggct
    21121 ggatcacggc ccccttcgtc gcgaaggaga tcgtcgacgg ctggctctcc atggccttcg
    21181 cgcagaagat ggagttcaag cgcgacttcc tcgcgaacgc cctcaaggaa gtgaaggcgc
    21241 tcgaagagaa gaacttcagg taaaggcggc gccccgatgc tctcctccct cctctccccc
    21301 tcgcgcgtcc tctccttcga ggagctgtcg cccgcggact tcgccaaggc cgcgttctac
    21361 ctgggcggtg cgccgcggaa ggcggccgtc gtcgtcgacg ggcgggcctt ccccgagcgg
    21421 gacgccctgg tcgcggggct tcaggccctc tgcgagaccg tcgtcctgga cagggtgcgg
    21481 cccgaccccc tcgcctccga cgtggacgcg atgcgcgaaa tgatcggagg gggggagctc
    21541 ggcctcgtcg tcgggatcgg cggcggctcc gtcctcgact ccgccaaggc cctggccatg
    21601 ctcgccgcga acggcggctc cttgagcgac tacctgggcc ccgccccctc gcggaagatc
    21661 gagaaaaagg gcccgcccct cctcctcgtg ccgacgaccg ccggcacggg ctccgaggcc
    21721 acgaaggtcg gggtcttcac ctcggactcc ggccggaagt acaccctggg ctcgcccctc
    21781 ctccaggccg acgccgcgat cctctcgggc tcgctggccg cggggatgcc tcccgccctg
    21841 gccgcctcca ccggcttcga cgcgctttcc cacgctctcg agtccctctg gaaccggaac
    21901 gcgactcccg tgaccgtcca ggcggccgtc gacgccgccg tcgccgtcct gcgcggcctc
    21961 gagcccgcct acgacgcggc cgtcgccgcc gccgcgggca agcccctccc gggcgccaag
    22021 gccgcggccc tgcgcatgct cgaggccgcc tccatggccg gcttcgcctt cagccgcacg
    22081 ggcacggcga tggtccacgc cctctccttc gtcctctccg aggaatggca cgtgccccac
    22141 ggcgcggcct gcgccttcac cctggaggac gcctaccgct acgccctctc cgagcccgag
    22201 aaccgggccc gcctggcgcg gatcggcgag gccctcttcc ccgaggcggc cgcgcggagc
    22261 gacgccaagg gcggcgctac cgaatccgcc gcggaccgcg acgagcggct cgccggcctc
    22321 ctcctcgagc gcgtcctcgt cctcaagcgc aagatgggcc tcccggagcg cttcgccgac
    22381 ctcgggatct ccgtggaccg cggcgcgatc ccgggcctct tcgcccgctc cttcgacgac
    22441 ccgaagatgg cgaacaactt ccccgccgcc gagcggccgg cggtctacgc catcctcgag
    22501 gccaaggctt gagcctgggc gccctcctcc tctcgggcct ggtcgtcctc gcgacccacg
    22561 ccctggaggc gatcaccggc ttcggctgca cggtcctggc cctgcccttc gtgacggcca
    22621 tcctcggcct caagaccggc gtgatggtgc tcacgatcct ggcctggatc ctcgccctct
    22681 acatcgccgt cacgaagcgc cgcgacatag atttcaagca gtacgggatc atcctcggct
    22741 tcgtcctcct cggactgccc ctcggaatgt acctcttcag gacggtggag gcgggctcct
    22801 tgaagcgcgt gctcgcggtc ttcatcgtcg ccgcctccgc cctccagctg gcgaagcccg
    22861 cgctggcccg cggagccgag cggcgggccg cccaggccgg gccgagtccc gccgccggct
    22921 caggaggcga ggtccgcgga gagggcgaag cctgcgcggc ggcccttgtc cccggcgccc
    22981 cccgctggct ctactatctc ctcctcgtcg ccggcggcgt cgtccacggc ctcttctcct
    23041 cgggcgggcc cttcgtggtc ctctacgcgg ccaaggccct gcccgacaag gggaagttcc
    23101 gcgcgaccct ctgcctcctt tggacgaccc tcaacacggt gatcatcgcc tcgtacttcg
    23161 tggcggggac ggggatcggg gcaggcgccg cgaaggccac ggccgcgatg ctgcccttcc
    23221 tggccctcgg catcctcgtc ggcgagcgcc tgcacaagaa ggcgacgccg gccgtcttct
    23281 cgaccctcgt cttcgcgatg ctcctcgcga cgggcgtcgt catgctcttc ttctaggggg
    23341 taccatggac cgcgccggcc tcgccgccag gaatccgcat ctctccgtcc tcggtccggg
    23401 cgaccccggc ctcgccgcct acggccgcgt cctggacctg cccggcctcg aaggcctcgt
    23461 cgggctcgcc gaccggatcg tgccggccgg cctcgaggcg aacgcctatg tcgcctcgga
    23521 ggccgagctc gagtcccatc ccgccgccgc ggccttcggg cccgccttcg gcttcgccga
    23581 gctccaggtc ggctggaacg ccggccccaa ctcgcggctc aacggcctcg agtggcacaa
    23641 gtcccccgag atcctcgtcg cggtgagcga cctcgccctc ctcctcgggc gcttcgagga
    23701 catcgagctc cgcggcccaa gcggcccgcg cttcgactcg agccgcctct cctgcgtctt
    23761 cctgccgcgg ggcgcggccc tggagctcaa gg
//
LOCUS       DAOOSJ010000010        23332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1516_length_23332_cov_6.482708, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000010 DAOOSJ010000000
VERSION     DAOOSJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 23332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23332
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_23332_cov_6.482708"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1400)
                     /locus_tag="PLG14_01180"
     CDS             complement(297..1400)
                     /locus_tag="PLG14_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOX47768.1"
                     /translation="MNIDQAAVDRAVAHALALCAIPSPSGMTAAAASYVEEALGALGL
                     SPERTRKGAVLCRLGGEGRPLVLAAHFDTLGAVVRSVKSNGRLRYSKIGGFPDLNLVG
                     ETCLVHCRDGRSYSGTFQPVEASVHVNQKLKELKPDEDSVEILVDELVRTREETLALG
                     IAPGDFVSIDARARLTPSGFLKSRHLDDKAASGILLALAGLVAGKTGPAAGPADSGAR
                     PDAAGAGKAAPLRPGRSVSLLFTAHEEVGHGGAPLPEGIEEFVSVDMGAVGEDLSCDE
                     RMVSICAKDSRGPYDFEVTTALVEAAKRAGCSYAVDVYPSYGSDADAALAAGHDLRHG
                     LVGPGVYASHGYERTHRDAMANTLALLAEYLRA"
     gene            1636..1959
                     /locus_tag="PLG14_01185"
     CDS             1636..1959
                     /locus_tag="PLG14_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 family protein"
                     /protein_id="HOX47769.1"
                     /translation="MPHIRARNVDPALLEARARVLVDRLQEAVGCDRAWFTLELEASR
                     AIDSGSGEPVRPYVEVHWFPRPPEAKKAVARILSEELRGGADYLTTVFFDLAEADYFE
                     NGEPV"
     gene            1962..3680
                     /locus_tag="PLG14_01190"
     CDS             1962..3680
                     /locus_tag="PLG14_01190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX47770.1"
                     /translation="MDTSGRGGPGEEAGGIPIEPGSDEEPRAAQREASHEAQREAPRA
                     AAPGCFRLRLPDPSTGEARASYFLGAGPGGVLVDPGGAADAEALFAGLKALGGTAGLA
                     AVVLTDPRPETAAGLLRLEAEGFRGQVYLRGEWLGAARAYGFASEIRPLGAGPERVEL
                     ASGRSLRFFASAVPAPHGSLLCLDETTGTLLSGERFSPSGESGAQGAQGEPFLLARLA
                     ELARRGELARIAPSRAPVLAEAVADFIERFASAEALLAGACADPDAAEARADELAEVE
                     RSISSDPLTRLKNQRFLHSLLLDRLKAQGGRLEAALLFVSLDGLVEINRELGREGGDE
                     ALKSLGYILGNDAAAFPGTLAFKLNSPAFVVLVPGGERAAAAAAAERIRKTVRESEVS
                     SRSFTVSIGLVYPDEATGSGDAAIRRLEEIGGVRGLLAKERGGDTVCDSSTEHDAELQ
                     LSKRIVLVEPETAHAAFLAARLEGRGFRVAVFPEGRSALERILADGCDLVVSEAMVPR
                     LSGFQLREALMADSARSRIPFVLVSHRKDDEYLEKAASLRITHYLKKPYALAEILGLA
                     ENLARG"
     gene            3702..6518
                     /locus_tag="PLG14_01195"
     CDS             3702..6518
                     /locus_tag="PLG14_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX47771.1"
                     /translation="MSLVALALGAQAAFALALVLRLAFVRARDARAARTRALLSEAWP
                     ALEAGAAPPAALASRPRDFLEALVELRQSVAPSKAALAALAASFSASGYDLRLVRRLL
                     RGGPDARAFAASRLAYVGSPAAARALSLAILREPPGPVRVAIAEALGVIGDPVFLPSV
                     VDALKGSSPEDRARVGAIAAGFGDEFLSYAPSLAERREPEIQELLFEASTRSPSEILG
                     AFLRSRLDDPDPATRKEARLRHLAAYASDAEAVWFSEDETDDPEVRRAAIRRLGDSPS
                     VSNFEFLVGLLSEPAVRADARAALSDLVVASPALFGRLVDRLRAEPDGAARGRLLAVV
                     SLRIEYCLARLGDDPDRRFAEALESVLAGGKTAGLVNFLNRNRDEAVEEAVLAVLSRR
                     LRAGESLGEEMEERLDPRILSRLGLERRVPSAERAARNERVDPKPLALVLFLVVTLPL
                     GAYLAASGGRPTAAGFALTFLRAFAAYALALNGLTVLLAALSFAESRRHAGRLGRKTA
                     SLLFREKMLPSISVIAPAYNEEASIVESVLSLLNLRYPDYEVIVVNDGSRDRTLDLLV
                     GTFELVKRDVVLGTSLKTRRIRGVYSSKRWPGLLVVDKENGGKADSLNAGINAARKRL
                     FAGIDSDSLLERDSLLRVAAAWLDYREPVVAAGGNILPANGCEVAYGDLAAVRAPRRA
                     LPLFQSVEYLRSFMNGRLGWSALRSLLIISGAFGLFDRRQVVEAGGYLTSRERFLKDT
                     VGEDMELVVRITRTMREAGRPFRVHYESAANCWTEAPERLAVLKRQRDRWQRGLLDIL
                     AFHRKVMFNPRYGAMGLLGFPYFLVFEIAGPFYEFAALLLSVAAPWLLPGWPPVVLAA
                     FAASVLLGSAASLLSLLIAEWDGPIYGPRDHLLLAGAAIAENFGFRQLMGIFRIGGFA
                     SALRGASGWGAMTRSGFAAKGAKT"
     gene            6725..7396
                     /locus_tag="PLG14_01200"
     CDS             6725..7396
                     /locus_tag="PLG14_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47772.1"
                     /translation="MRSITTLELKTVASGDLEGQAESVIVVDLKGPLLLSYRQAYEKA
                     SGGDVHRFQPGLVAVFAPGLYAELVAGPLLDGEGEVGVDGFLEATYEFGKASAYARAK
                     LRDWPSAGSYLTIASVGGSYDFLSWYTVRARFFYGWNEAGESSFSSVFTNEFAVGPAF
                     GVQAGWELGYERLEASESLPWGLSLGLRYDLARGLRLKALGEYKADSGGLRSAGGSLV
                     ADWRF"
     gene            complement(7497..9149)
                     /locus_tag="PLG14_01205"
     CDS             complement(7497..9149)
                     /locus_tag="PLG14_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX47773.1"
                     /translation="MNRKPSGAPGAVPLFGAALCAAAVCLALAACAAPKGAAGPGGPL
                     PSEAPRYVESQKSMESLAPPPPSPSMSRAMAPSAKAAADQGSSGAAAGGQASADEYKG
                     IVENEFKEAAKEPLSTFAADVDTASYANVRRFVAEGSLPPPDAVRIEELVNYFDYGWA
                     EPKAVDGRPAPPLAATVEAAPAPWAPERRLLAIGLRTRSVAAENLPPANLVFLVDSSG
                     SMSSPDKLPLVQRAFSLLADTLRPQDRVAIVAYAGSAGVVLPPTSGSEKTKILEALER
                     LSAGGSTAGGEGIVLAYETALANFRKGGNNRVILATDGDFNVGPSSEKELLALVEDYR
                     KKGVFLSLMGFGRGNLKDSRMEALADKGNGNYSYVDTLAEARRLLVEEMGATLLTVAK
                     DVKLQVEFDPKAVRSWRLVGYENRLLAAEDFADDTKDAGEMGAGHRVTALYEIEPEPG
                     ASPRSLGVLRVRYKKPAEEQSALLEFPLLDEGRGADGASADWKLASAAAAYGLVLRDS
                     KHKGRADFGLVLSLAKEAAKAAPSDRRLEFVGLVEKAAALKR"
     gene            9339..10049
                     /locus_tag="PLG14_01210"
     CDS             9339..10049
                     /locus_tag="PLG14_01210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOX47774.1"
                     /translation="MRVLQLIHWEPKFEYNCSGYFARRGDRVDNAQLFKGGPAPEPED
                     YDALIVYGGYMSAFDDSGHPWIKDELRFLESCLKAGLPVLGICLGSQLLARLLGARIY
                     KSDFPEFGFKRLELTEEGAADPALGRLAGQGGRGFLALEWHNDAWDLPAGAVRLARSE
                     AWENQAFRYGPKVLATQFHLEFTQPHMAAGVAANSGELPADPAAEAPEAFVAPGPRWE
                     EIRLNMERLLDGFLEGAR"
     gene            complement(10225..11679)
                     /locus_tag="PLG14_01215"
     CDS             complement(10225..11679)
                     /locus_tag="PLG14_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47775.1"
                     /translation="MRKQKSLDVMSSAAETAGAGASGAAGPDRADAAPAGAAASVSKG
                     SGALPGRLWNRDFTLLWQGQFVSSLGKQAFGLAAVLWLKEATGSGTIMGFVMMAATLP
                     AVLLGPVAGVAVDRFDRRKIIAYTDLAGGLFVLLAALLFLLRPEATGLLIATVFAVTL
                     ATGLLDTFSQPSIGASLPDLVPKERLEAANGLNMAGVQLAVFGAQGSSGILYAILGAP
                     LLVLWNAATYLYAGFTELFIRVPKSAAKDAEREARLAALHPFHRFRLELGEGLSFLRG
                     HRGMRTLIVVFALLNFFISPVLVVLPFFAQDFLGLGPAWYGYLMASYGLGSLFGYLAA
                     GALPFRGKARELAVNGCLLAQSLLMVLCVFWKAPAAELVLFVLIGLSGGVLNVNAISA
                     IQLATPPEFRGRVQALVMTACAGAMPLGMAVSGLLFDLSGKNVALMFGASAGLTLLAT
                     LGALAVKDYRGFLAGEEDASQVAPPAGGAPNAGA"
     gene            11795..13294
                     /locus_tag="PLG14_01220"
     CDS             11795..13294
                     /locus_tag="PLG14_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47776.1"
                     /translation="MHKISKGLAILAAALLCSCAGGPGGSKPAYDADVIVVGSGLAGH
                     GAVYGALEGGARVLLLEKNDRLGGTSLTSTGTFSAAGTRIQKEKGIEDSVQAHIDDIN
                     RIGHGKADQALLRKFAEEAAGVWEWFVDKGINPNPAGPVIDPVHSPYSVARTMTPAKN
                     SANEYVRVLAEQAKAKYGERLAALVSTKVTALVSEKGRVVGVKAAGPEGERIYRAKAV
                     VLATGGYGSNRAMIQKYSPRYAEYRTVTPPWATGEGIEMAAAAGAELVNMDYVVGYFG
                     GMPKAADHYALGFGDLTSGFADRWKGEIWVGSDGKRFVNEDDGDEDPRETAIDKLEGG
                     KILIVFDQGILDRNGGKTPIRDFDARLAQGYAVKKADTVEELAKAFGVDAKGLKATID
                     GVSAASAQGQADYFGKTTGLPFEKGPYYGVLCYGTIFMTQGGVRVDGSLRALGKDGKP
                     VAGLYAAGEAMGTSQWGGRGYAGGTGVAPAIVFGLDAGKSAAAFAKAGK"
     gene            complement(13364..13612)
                     /locus_tag="PLG14_01225"
     CDS             complement(13364..13612)
                     /locus_tag="PLG14_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOX47777.1"
                     /translation="MAISISSPLPGLPAGKPAGLPCPFLDESLRCALYGRPERPAVCA
                     SLKPLPEMCGSSREEAMAYLSELERLTAPEASGDGEKA"
     gene            complement(13657..13739)
                     /locus_tag="PLG14_01230"
     tRNA            complement(13657..13739)
                     /locus_tag="PLG14_01230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13704..13706),aa:Leu,seq:caa)
     assembly_gap    13740..13839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13840..15818
                     /locus_tag="PLG14_01235"
     CDS             <13840..15818
                     /locus_tag="PLG14_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOX47778.1"
                     /translation="FRARVARLLVLASLGLGLVLLVPSLADAWATARYALFAADLVAY
                     FGLLCLAVLDRGRRRFSSYGIVLLAFAQGFFLYAYRGPAGAGESWFCSAVVLAAILFG
                     ARGGLALAGIVSLSFGGLGLAAVRGLLRWSAAGSYLAILALNTVTLALVAGLAAASLV
                     RSLRKALEAGERLRAELAEGKAALEREAEERRGAERLAEFYRDHDGRTGLPNRERFLY
                     ELGRAARSAERRKRLLAVLAVGVDEPGRLYDEYGHAASEAALAEAALALKESFREDDL
                     VARFDETHLFVLCENLNDSGDAGELIRKAKGSFARPFRAEGAATAAATDGAVAGPAGA
                     AGPAMAAAGAGMSLSASVGLAFYPHDGAGPEALLEAARAALRLAEESGPGSYRLFDAR
                     LHAGLIARIRYGRELEAAVEEGLLVPWFQPKVDAGRRIVGAEALARWPRDDGPTRMPS
                     DFIPAAERQGAIGRLGLRMLRLSCLRAAAVERASGRGLVMAVNVSPLQLADPGFPAEI
                     RAILAETGLAPRLLELEVTETGLASMEGQAALRLAELKALGLRLAIDDFGTGASAIAR
                     LKDYPVDSVKIPKQFVDHLPEEGRAPTIVRAVIDLAHRLGFATVAEGVETEAQFEWLK
                     SASCDEFQGFLFSRPLPADAFEAELARAFPEAVE"
     gene            15824..16723
                     /locus_tag="PLG14_01240"
     CDS             15824..16723
                     /locus_tag="PLG14_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX47779.1"
                     /translation="MESILSWGVELIRAVQRHASPALTIAMKGLTALGSEWFYLAVLP
                     VVYWCVDRRKGQRLGVVFLASAFLNLWLKDLFAQPRPYDLDPSVGMARESSHGLPSGH
                     SQNSAVFWGLAAPQVRRPWGLVLALALPLLIGFSRIYLGVHFPTDVFAGWALGGLFLG
                     AYALAGHRAERALDRLDVRAKVALVAGLAFAMNALEMRDTSIAGGFFGMGIGLVYAGR
                     LAPFSAGGPLGKRALRFVFGMAGAAVLYLGLKAVFPGKGSELYALFRFLRYGLLGAWV
                     ALGAPWAFLRLKLAEREDLSARG"
     gene            complement(16725..17744)
                     /locus_tag="PLG14_01245"
     CDS             complement(16725..17744)
                     /locus_tag="PLG14_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOX47780.1"
                     /translation="MRKQALPAALAAFLLLSCAKEAPAEDARTEFVLGTVCSLRLVSG
                     GDAKTVDAVFARLRGIEDRMSANKEGTEIAAVNAAAGLAPVRVSADTFYVVKKGLEYA
                     RLTGGAFDPTVGPLVKLWGIGTDAARLPSPAEIKAATRLIDYRKVGLDEAASTVFLAE
                     KGMRLDLGAIAKGYAADEAKRVLAERKVKAAIIDLGGNVFAYGQKKDGSPWRIGVQDP
                     ASDRGDYIGIATCKDETVVTSGVYERYFEEGGKRYHHILDTRTGYPVENGLVSVSIIS
                     SSSIDADGLSTSLFALGREAGLRLAATLPGVEVVMIDAERRVWLTPGATKVFRITSGN
                     YRLAE"
     gene            complement(17813..18481)
                     /locus_tag="PLG14_01250"
     CDS             complement(17813..18481)
                     /locus_tag="PLG14_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX47781.1"
                     /translation="MIERLTVGPIGENAYVLPLDGGAGLPREGGPCVLVDPGDEAERI
                     LDFLDSRRLVPSLVVATHGHLDHVGALPSLMAAFAARGRALLLAVHRADAAYFGERGE
                     ETNRRVFQAIRAMGYFKSYWKTMPEAGLLLEDGALLPGSTWRVIHTPGHTAGSICLYD
                     EARGVLVSGDTLFRDGVGRTDGPDSDPAALEASLKERLFLLPVDTRVFPGHGEPTTIG
                     RERG"
     gene            18524..19753
                     /locus_tag="PLG14_01255"
     CDS             18524..19753
                     /locus_tag="PLG14_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47782.1"
                     /translation="MFIPLYAVLAAALVFYLLLPEAAVLSRRLSWRRFRRRLEALSRA
                     PLLRYRDAVPPRALLEGQAGAAGQAGAAPEGRAAARGEGGAGDGGRGEARGPFRLYGR
                     IEAMEGDDRVWLRGKEVSALVDLSRSPLYVLSPGPEEEAGAGSGERLERISWRRVRVL
                     SEGTRLMAAGPLRFEGGRPVFAEAPGEPLVAVIHGGGEGDLLSRLVAGARRPALAGGP
                     FATVSVAFGLALSSALLFLLAEGRGVLPTVRVLAYLAALSPLLPFLPPGLALYLLYRR
                     LAREAHGLRVARDLLRLPLRFFGSPDLAEARLACGSPYRRSLGGPASGPPPPGYGPVA
                     LPLKPGAGASWTLFGPEEGCEDPAAERVAVPGEPELLAERCAGKARRLGLGAVLALAA
                     GGALNFGLVFAVWRSLP"
     gene            complement(19819..20727)
                     /locus_tag="PLG14_01260"
     CDS             complement(19819..20727)
                     /locus_tag="PLG14_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOX47783.1"
                     /translation="MPKPRPAAEAPAGAALAHIDRILAELSSPSDPEALNEVRAAFRK
                     KVPLHLRSYAAAALLLEASRQGAQRGDRRRDEPKAGGKRRGKGESARGESSRGDEPRP
                     ESQRGESRGEGRGARRERAPEPREAAPEEQRPRLQGEGTTIFFGMGKRQRLYSRVLLR
                     VLSEEGGLALEDIGDIRSFDNYSFADVAPERAEELIAALASFEFRGRVLPVNKARKRG
                     EAAPEPREDRGEGGRRDEGEALAVEAGFDESLEDASMDDEAYGDEAPLGEDLAEDDGS
                     DASYDEEPADEPEDDLDEEKDEDKAL"
     gene            20977..21588
                     /locus_tag="PLG14_01265"
     CDS             20977..21588
                     /locus_tag="PLG14_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47784.1"
                     /translation="MLPQKLPLVPAGLLCLLLLAPPLEAEASSGPAGPAAANASAAPA
                     ATGLGSPGPSAAAQSGGAAPGASASLPSCPILPDDPAPLLGLSLAESYLRLGAPASVA
                     AFRGVEAWQDDVVFSFGSGYSLFWFGDRLWQIRFTPGYGGSVYGLFLGDGKDKVYSLL
                     GAPFYEAEGSLVYRLPYRGYPVRLRVVVAEGRVADLYLYRADF"
     gene            21597..23093
                     /locus_tag="PLG14_01270"
     CDS             21597..23093
                     /locus_tag="PLG14_01270"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOX47785.1"
                     /translation="MSLVCLSLTGKSLAEDLAVLDLYRGQVDLVELRADYLDPSEKFL
                     IRSFPERAGLPCILTVRRRVDGGAFAEGEGVRLVMIAKGLAYARSERLANFAYVDLES
                     DFRIPAVEEACRAFGTRIIRSVHSPKSLPADLDALWDELAETPDEIPKLALACESASD
                     LSRLFFWAESLPPRERIVAGMGDYGFPSRILAERTGSLLTYASALEAGLPHAAPGHLD
                     PEALASTYRFREIGKATAVYALTGGRSVVGARSPALHNAAFRAAGMDAVYLPFPAEDA
                     AAFLAAAEAVGVRGAAVTVPHKEAFAPLMARLSPEAAEIGACNTLFRGPEGWEGSNTD
                     APGFERALHEFLGRTDLSGLRATLVGAGGAAKAVAQVFAKSGVSCLVLNRSAAKAKAL
                     AKARGFAWAANDERAAELVADHADLIVNATSVGMDGGPPGDPLEWYDFTGREAVFDLV
                     YRPDRTAFLKRAKTSGARISNGWTMLRYQAAEQFRIWTGLEPPRTYFE"
     gene            23102..>23332
                     /locus_tag="PLG14_01275"
     CDS             23102..>23332
                     /locus_tag="PLG14_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOX47786.1"
                     /translation="MAKSAFVAIAGRPSAGKSTLVNALCGGKVSIVSPVPQTTRNAVR
                     GIVNKPEGQLVLLDTPGFHLSDKRLNKMLKNVA"
BASE COUNT         2960 a         8524 c         8466 g         3282 t
ORIGIN      
        1 cgcgcttcgg cgaggtcctg cgcctcctcc ccgacgaccc ggtcaccctc atcttcctgg
       61 agcggagcac ggtcttcgcc tcctccccgc ccccgcccga gtgggacggg gtcgtctccc
      121 acctggagaa ataggggcgc ggggacaccc ggaccccgcg gccccgcgcc gccgcggagg
      181 ccgagacccg cctcggcgcc gggcgggtcg aacggcggcg cggcgggccg aacggcggcg
      241 gctcgggccg ggtcgcgccg ggacgcagcc ggccgcggag gccgccagag ccgctcctag
      301 gcccgcaggt actccgcgag gagggccagg gtgttggcca tcgcgtcgcg gtgggtccgc
      361 tcgtagccgt ggctcgcgta gacgccgggc ccgacgaggc cgtgcctgag gtcgtggccg
      421 gcggcgaggg ccgcgtcggc gtcggagccg tagctcgggt agacgtcgac ggcgtaggag
      481 cagccggccc gcttcgcggc ctcgacgagg gccgtcgtca cctcgaagtc gtagggcccc
      541 cgcgagtcct tggcgcagat ggagaccatg cgctcgtcgc aggagaggtc ctcgccgacg
      601 gcgcccatgt cgacggagac gaactcctcg atcccctcgg gcaggggggc tccgccgtgg
      661 ccgacctcct cgtgggcggt gaagaggagg ctgacgctgc ggcccgggcg gaggggcgcg
      721 gccttcccgg cgccggccgc gtcgggacgc gcgcctgagt ccgcgggccc ggccgcgggg
      781 cccgtctttc ccgcgacgag gccggccagg gcgaggagga tgcccgaggc ggccttgtcg
      841 tcgaggtggc ggctcttgag gaagcccgag ggcgtgaggc gggcccgggc gtcgatggag
      901 acgaagtcgc cgggggcgat cccgagggcc agggtctcct ccctcgtccg gacgagttcg
      961 tccacgagga tctccacgga gtcctcgtcg ggcttgagct ccttgagctt ctggttcacg
     1021 tggacggagg cctccacggg ctggaaggtg cccgagtagc tccttccgtc gcggcagtgg
     1081 accaggcagg tctccccgac caggttcagg tcggggaagc cgccgatctt cgagtagcgg
     1141 aggcggccgt tggacttcac cgagcggacg acggcgccga gggtgtcgaa gtgggcggcg
     1201 aggacgaggg gccggccctc gccgcccagg cggcagagca cggccccctt gcgcgtgcgc
     1261 tcgggcgaaa ggccgagcgc gcccagggct tcctccacgt agctggcggc cgcggccgtc
     1321 atgcccgagg ggctggggat ggcgcagagg gcgagggcgt gggcgaccgc ccggtcgacg
     1381 gcggcctggt cgatgttcat ggagggctcc ttttcgccgc ccagtgtagc cgggccgggc
     1441 gcctgggtac aggcgggcgc gggcggagcg gcccggaagg cgcggaccct cggagcgggg
     1501 caaccggcgg ccgcgctggc ggcggggggc ccggggctgg cggcccgcgc cggcggactg
     1561 cgccgcggcg gcggcccgcc cgggccgttt gactccgcgg ctcggggagg ctagcattgg
     1621 cgccaggagc aagccatgcc gcatatccgc gcgcgcaacg tcgatccagc cctcctcgag
     1681 gccagggccc gcgtcctcgt ggacaggctc caggaggccg tcggctgcga ccgcgcctgg
     1741 ttcaccctgg agctcgaggc ctcgcgcgcc atcgactcag gctcgggcga gccggtccgg
     1801 ccctacgtcg aggtgcactg gttcccccgc ccccccgagg cgaagaaggc cgtcgccagg
     1861 atcctctccg aggagctccg cggcggggca gactacctca cgacggtctt cttcgacctc
     1921 gcggaagcgg actacttcga gaacggcgag ccggtctagc gatggacacg agcggaaggg
     1981 gcggtcccgg cgaagaggcg ggcggcatcc cgatcgagcc gggctcggac gaggagccgc
     2041 gggcggccca gcgcgaggct tcgcatgagg cccagcgcga agcgcccagg gcagccgcgc
     2101 ccggctgctt caggctccgc ctgcccgacc cctccaccgg ggaggctcgg gcctcctatt
     2161 tcctgggcgc cggcccgggc ggcgtccttg tcgatccggg cggggcggcc gacgccgagg
     2221 cgcttttcgc gggcctcaag gccctgggcg ggacggccgg cctcgcggca gtcgtcctga
     2281 ccgatccccg ccccgagacg gcggccggcc tcctccgcct ggaggccgag ggcttccgcg
     2341 gccaggtcta cctacgcggc gaatggctgg gcgctgcccg cgcctacggc tttgcgagcg
     2401 agatccggcc gctgggcgcg gggcccgagc gcgtcgagct cgcctcgggc cggagcctca
     2461 ggttcttcgc ctcggccgtt cccgcccccc acgggagcct cctctgcctg gacgagacga
     2521 ccggcaccct gctctcgggc gagcgcttca gcccctcggg cgaaagcggc gcgcagggcg
     2581 cgcaaggcga gcccttcctc ctcgcgcgcc tggcggagct ggcccgccgc ggcgagcttg
     2641 cgaggatagc tccctccagg gcgcccgtcc tggccgaggc cgtcgccgac ttcatcgagc
     2701 gcttcgcttc ggccgaggcc ctcctggcgg gcgcctgcgc cgacccggac gcggccgaag
     2761 cgcgcgccga cgagctggcc gaggtcgagc gcagcatctc ctccgacccc ctgacgcggc
     2821 tcaagaacca acgcttcctc cactccctcc tcctggaccg gctgaaggcc caagggggca
     2881 ggctggaggc ggccctcctt ttcgtctccc tggacggcct cgtggagatc aaccgggagc
     2941 tcgggcgcga gggaggggac gaggccctga aatccctggg ctacatcctg ggcaacgacg
     3001 ccgcggcctt tccgggaacc ctcgccttca agctcaactc gcccgccttc gtcgtcctgg
     3061 tccccggcgg ggagagggcg gcggccgccg cggcggccga gcggatcagg aagacggtcc
     3121 gcgagagcga ggtctcgagc cgcagcttca cggtctccat cggcctcgtc tatcccgacg
     3181 aggcgacggg ctcgggcgac gcggccatcc ggcggctgga ggagatcggg ggcgtccggg
     3241 gccttctcgc gaaggagcgg ggcggcgaca cggtctgcga ctcctccacg gagcacgacg
     3301 ccgagctcca gctgtccaag cggatcgtcc tggtcgagcc cgagacggcc cacgcggcct
     3361 tcctggccgc ccgcctggag ggcaggggct tcagggtcgc cgtcttcccc gagggccgct
     3421 cggccctgga gcggatcctg gccgacggct gcgacctggt cgtgtcggag gcgatggtgc
     3481 ccaggctctc gggcttccag ctcagggagg ccctgatggc cgactcagcc cgctcgcgca
     3541 ttcccttcgt cctcgtctcg catcggaagg acgacgagta cctggagaag gcggcctccc
     3601 tgcggatcac gcactacctc aagaagccct acgccctggc ggagatcctg ggcctggccg
     3661 agaatctggc gcggggctag ggggcgggtc tgcgctatcc cttgagcctc gtcgccctgg
     3721 ccctgggcgc ccaggcggcc ttcgccctgg ccctcgtcct gcgcttggcc ttcgtccgcg
     3781 cgcgggacgc cagggccgcg cggacccggg ccctcctctc ggaggcctgg ccggccctgg
     3841 aggccggggc cgcgccgcca gcggccctcg cctcgaggcc gcgggacttc ctagaggccc
     3901 tggtcgagct ccggcagagc gtggccccct ccaaggcggc gctcgcggcc ctcgcggcct
     3961 ccttctcggc ctcgggctac gaccttcgcc tggtgcgtcg cctcctccgc ggcgggcccg
     4021 acgcgcgggc cttcgcggcc tcccgcctgg cctacgtcgg cagccccgcg gccgcgcggg
     4081 ccctctccct cgcgatcctg cgcgagccgc ccggccccgt ccgcgtcgcc atcgccgagg
     4141 ccctgggggt catcggcgac cccgtcttcc tgccgagcgt cgtcgacgcc ctcaaaggct
     4201 cgtcccccga ggaccgggcc cgcgtcggcg cgatcgcggc gggcttcggc gacgagttcc
     4261 tctcctacgc gccgagcctg gccgaacgcc gggagcccga gatccaggag cttctcttcg
     4321 aggcctccac ccgctcgccc tcggagatcc tgggcgcctt cctgcgctcg cgcctggacg
     4381 atcccgaccc ggcgacgcgc aaggaggcgc ggctcaggca cctggccgcc tacgcctccg
     4441 acgccgaggc ggtgtggttc tccgaggacg agacggacga ccccgaggtc aggcgggcgg
     4501 cgatccggcg cctgggggac tcgccgtcgg tctccaactt cgagttcctg gtcggcctcc
     4561 tctccgagcc cgccgtccgc gcggacgccc gcgccgccct ctccgacctg gtcgtcgcct
     4621 cgccggccct cttcgggcgc ctcgtcgacc ggctgcgcgc ggagcccgac ggcgcggccc
     4681 gcgggcgcct cctggccgtc gtctccctgc gcatcgagta ctgcctggcc cgcctgggcg
     4741 acgatcccga ccgccgcttc gccgaggccc tggagagcgt cctcgcgggc gggaagacgg
     4801 ccggcctcgt caacttcctg aaccgcaacc gcgacgaggc ggtggaggag gcggtcctgg
     4861 ccgtcctctc gcggcggctc cgcgcgggcg agtccctggg cgaggagatg gaggagaggc
     4921 tggatccgcg catcctgtcg cggctcggcc tggagcggcg ggtgccctcc gccgagcgcg
     4981 cggcgaggaa cgagcgcgtc gacccgaagc cccttgcctt agtcctcttc ctcgtcgtaa
     5041 ccctgcccct gggcgcctac ctcgcggcct cgggcggcag gcccacggcg gcgggcttcg
     5101 ccctgacctt cctacgcgcc ttcgcggcct acgccctggc cctgaacggc ctcaccgtcc
     5161 tcctcgcggc cctctccttc gccgagtcga ggcgccacgc cgggcgcctg gggcgcaaga
     5221 ccgcgagcct cctcttccgc gagaagatgc tgccctccat ctccgtgatc gcccccgcct
     5281 acaacgagga ggcgagcatc gtggagagcg tgctctccct cctcaacctg cgctatcccg
     5341 actacgaggt gatcgtcgtc aacgacggct cccgcgaccg caccctggac ctcctggtcg
     5401 ggaccttcga gctcgtgaag cgcgacgtcg tcctgggcac ctccctcaag acccgcagga
     5461 tccgcggcgt ctactcctcc aagcgctggc cgggcctcct cgtcgtggac aaggagaacg
     5521 gaggcaaggc tgattcgctc aacgcgggca tcaatgcggc caggaagcgg ctcttcgccg
     5581 gcatcgactc cgacagcctc ctggagcggg actccctgct ccgcgtcgcg gcggcctggc
     5641 tcgactaccg cgagcccgtc gtcgcggcgg gcggcaacat cctgcccgcg aacggctgcg
     5701 aggtggccta cggggacctg gcggcggtgc gggcgccgcg gcgggccctg cccctcttcc
     5761 agtccgtgga gtacctgcgc tccttcatga acgggcgcct gggctggtcg gcgctccgct
     5821 ccctcctcat catctccggc gccttcggcc tcttcgaccg ccgccaggtc gtggaggcgg
     5881 gaggctacct cacgagccgc gagcgcttcc tcaaggacac cgtgggcgag gacatggagc
     5941 tagtcgtgcg cataacgcgc accatgcgcg aggcgggcag gccgttccgc gtccactacg
     6001 agtcggcggc gaactgctgg acggaggcgc cggagaggct cgccgtcctc aagcgccagc
     6061 gcgaccgctg gcagaggggc ctcctggaca tcctggcctt ccaccgcaag gtcatgttca
     6121 acccgcgcta cggggcgatg ggactcctcg gcttccccta cttcctcgtc ttcgagatcg
     6181 cggggccctt ctacgagttc gccgccctcc tcctctccgt cgcggcgccc tggctgctgc
     6241 cgggctggcc tcccgtggtg ctggccgcct tcgcggcgag cgtcctcctg ggctccgcgg
     6301 cctccctcct ctccctcctc atcgccgagt gggacgggcc catctacggc ccccgcgacc
     6361 acctcctcct cgcgggcgcg gccatcgcgg agaatttcgg cttcaggcag ctcatgggta
     6421 tattcaggat cggcggcttc gcgagcgccc tgcggggcgc ctcgggctgg ggcgccatga
     6481 cgaggagcgg cttcgccgcg aagggagcga aaacatgagg ctatcttcga cgcgcctggc
     6541 ggccttggcc gcgacgctgt gcctagccct ggcgccggcc ttcggcgaag gggaggtcgc
     6601 tgccccagga gcgggcgcgg ccgccgcggt gcctgccgcc gcgtcgcctg gtgccgcgcc
     6661 cgaggcctcg cccgcgccag cgtccgccgc agaggtagcc gcccccgagg cggccagcgt
     6721 ccccatgcgc tcgatcacga ccctcgagct caagaccgtc gcctcgggcg acctcgaggg
     6781 ccaggccgag tcggtgatcg tcgtcgacct gaaggggccc ctcctcctct cctaccgcca
     6841 ggcctacgag aaggcctctg gcggggacgt ccaccgcttc cagcccggcc tggtcgccgt
     6901 cttcgcgccg gggctctacg cggagctcgt cgcgggaccc ctcctggacg gggagggcga
     6961 ggtcggcgtg gacggcttcc tggaggccac ctacgaattc ggcaaggcct ccgcctacgc
     7021 gcgggccaag ctccgcgact ggccctccgc cggcagttac ctcaccatcg cgagcgtcgg
     7081 cggctcctac gacttcctct cctggtacac ggtccgggcc cgcttcttct acggctggaa
     7141 cgaagcgggc gagtcgagct tctccagcgt cttcacgaac gagttcgcgg tcggccccgc
     7201 cttcggcgtc caggccggct gggagctcgg ctacgagagg ctcgaggcct ccgagtccct
     7261 gccctggggc ctgtccctgg gcctccgcta cgacctggcc cggggcctcc ggctcaaggc
     7321 cctgggcgaa tacaaggcgg actccggcgg cctgcgctcc gcgggcggga gcctggtcgc
     7381 ggactggcgc ttctagccgt agcgggccgg gccggcgagg cccggcgctt ccgggaagct
     7441 ctgcgccgcg aagccggccg cccccggccc gtgggccgag ggcggcgcgg cagaacctag
     7501 cgcttcaggg ccgcggcctt ctccacgagg ccgacgaact cgagccgccg gtccgacgga
     7561 gcggccttcg ccgcctcctt ggcgagggag aggacgaggc cgaagtcggc ccggccctta
     7621 tgcttcgagt ccctgaggac gaggccgtag gccgccgcgg cggaggcgag cttccagtcg
     7681 gccgaggccc cgtcggcgcc cctcccttcg tcgaggaggg ggaactcgag gagggccgac
     7741 tgctcctccg cgggcttctt gtagcggacg cgcaggacgc ccagggagcg gggcgaggcg
     7801 ccgggctcgg gctcgatctc gtagagggcc gtgacgcggt ggcccgcgcc catctcgccc
     7861 gcgtccttcg tgtcgtcggc gaagtcctcc gcggccagga ggcggttctc gtagcccacg
     7921 aggcgccagg acctcaccgc cttcggatcg aactccacct gcagcttcac gtccttggcc
     7981 acggtgagga gggtcgcgcc catctcctcc acgagcagcc tccgggcctc ggcaagggtg
     8041 tccacgtagg agtagttgcc gttgcccttg tccgccaggg cctccatcct cgagtccttg
     8101 aggttgccgc ggccgaagcc catgagggag aggaagacgc ccttcttccg gtagtcctcg
     8161 acgagggcga ggagctcctt ctcgctcgag ggcccgacgt tgaagtcgcc gtcggtcgcg
     8221 aggatgacgc ggttgttccc gcccttccgg aagttggcga gggccgtctc gtaggcgagg
     8281 acgatgccct cgccgccggc cgtggagccg cccgcggaga gccgctccag ggcctcgagg
     8341 atcttcgtct tctccgagcc ggaggtcggc gggaggacca cgccggcgga gccggcgtag
     8401 gcgacgatgg cgaccctgtc ctgggggcgc agggtgtccg cgaggaggga gaaggcgcgc
     8461 tggaccaggg ggagcttgtc ggggctcgac atggagccgg aggaatcgac gaggaagacc
     8521 aggttggcgg gcgggaggtt ctccgccgcg acgctccgcg tgcggaggcc gatggcgagg
     8581 aggcggcgct ccggggccca gggggccggc gcggcctcca ccgtcgcggc cagcggcggg
     8641 gctggcctgc cgtcgacggc cttgggctcg gcccagccgt aatcgaagta gttcacgagc
     8701 tcctcgatcc tgacggcgtc gggcggcggg aggctcccct cggcgacgaa gcgccggacg
     8761 ttcgcgtagg aggccgtgtc cacgtcggcc gcgaaggtgg agaggggctc cttggccgcc
     8821 tccttgaact cgttctccac gatccccttg tactcgtcag ccgaggcctg gcctccggcg
     8881 gccgcgccgg aggagccctg gtcggccgcg gccttggccg agggggccat ggcccgcgac
     8941 atggagggcg aggggggcgg cggcgcgagg ctctccatgg acttctggct ctccacgtag
     9001 cgcggggctt cggagggcag ggggcctccg gggccggccg cgcccttggg ggcggcgcag
     9061 gccgcgaggg ccaggcagac ggcggcggcg cagagggcgg cgccgaagag ggggacggcg
     9121 cctggggcgc ctgagggctt ccggttcatg catacctccg cggcccccgc agagccttta
     9181 ggcaggaggg ccacggggat gaagtatagc gcggcccggg ccgtcccgcc cggatgccga
     9241 gccggcgctt tacgagctga gggcccgagg ctacactgcc tcgtcctcgg gatcagggcc
     9301 gcaggcaggc ccgcgccccg gggaagaagg gataggaaat gcgcgtcctc cagttgatcc
     9361 attgggagcc caagttcgag tacaactgct caggctactt cgcgaggcgg ggcgaccgcg
     9421 tcgacaacgc ccagctcttc aagggcggcc ccgcccccga gcctgaggac tacgacgccc
     9481 tcatcgtcta cggcggctac atgagcgcct tcgacgactc gggccatccc tggatcaagg
     9541 acgagctccg cttcctcgag tcctgcctca aggcgggtct tcccgtcctg ggcatctgcc
     9601 tgggcagcca gctcctcgcc cgcctcctgg gcgcgcggat ctacaagagc gacttccccg
     9661 aattcggctt caagcgcctc gagctcaccg aggagggggc ggccgatccc gccctgggcc
     9721 gcctggccgg gcagggaggc cgcggcttcc tggccctgga gtggcacaac gacgcctggg
     9781 acctgcccgc cggggccgtc cgcctggcgc ggagcgaggc ctgggagaac caggccttcc
     9841 gctacgggcc caaggtcctg gccacccagt tccacctcga gttcacccag cctcacatgg
     9901 ccgccggcgt ggcggccaac tcgggcgagc tgcccgccga ccccgccgcc gaggctcccg
     9961 aggccttcgt cgccccgggg ccgcgctggg aggagatcag gctcaacatg gaaaggctcc
    10021 tggacggctt cctggaaggg gcccgctagg gcgagctcgc cgggcgagcc cgccgggcga
    10081 gctcgcccgg cgagcccgtc gggcatggct tggccaggct tgcaaggcat ccccctgcgg
    10141 cggcggcgca ggccgctggc cccgccgccc gcgcgcgccc agcgcgagcc cggcggccgg
    10201 ctgatcgacg ccggacctcc agcctcaagc gcccgcgttc ggcgctccgc ctgcgggcgg
    10261 agcgacctgc gaggcgtcct cctccccggc caggaagccg cggtagtcct taaccgcgag
    10321 ggcgcccagg gtcgcgagga gggtcagccc cgcgctcgcg ccgaacatca aggccacgtt
    10381 ctttcccgag aggtcgaaga ggaggccgga gacggccatg cccaggggca tcgcgcccgc
    10441 gcaggccgtc atcacgaggg cctggacgcg gccgcggaac tccggcggcg tcgcgagctg
    10501 gatggcgctg atcgcgttga cgttgaggac gccgcccgag aggcctatga ggacgaagag
    10561 gacgagctcc gccgcgggcg ccttccagaa gacgcagagg accatgagga gggactgggc
    10621 caggaggcag ccgttgaccg cgagctcgcg ggcctttccg cggaagggca gggcccccgc
    10681 ggccaggtag ccgaagagcg agccgaggcc gtaggaggcc atgaggtagc cgtaccaggc
    10741 cgggccgagc ccgaggaagt cctgcgcgaa gaagggaagg acgacgagga cggggctgat
    10801 gaagaagttg aggagggcga agacgacgat gagggtccgc atcccgcggt ggccgcggag
    10861 gaaggaaagg ccctcgccca gctcgagcct gaagcggtgg aaggggtgga gggcggcgag
    10921 ccgcgcctcc ctctcggcgt ccttcgcggc gcttttcggg acgcggatga agagctccgt
    10981 gaagcccgcg tagaggtagg tggccgcgtt ccagaggacc aggaggggcg cgccgaggat
    11041 ggcgtagagg atgcccgagg agccctgggc gccgaagacc gcgagctgga cgcccgccat
    11101 gttgaggccg ttcgccgcct cgagcctctc cttgggcacg aggtcgggca ggctcgcgcc
    11161 gatggagggc tgggagaagg tgtcgaggag gcccgtcgcg agggtcaccg cgaagaccgt
    11221 cgcgatgagg aggcccgtgg cctccgggcg gaggaggaag aggagggccg ccaggaggac
    11281 gaaaaggcct cccgcgaggt ccgtgtaggc gatgatcttc cgccggtcga agcggtcgac
    11341 cgcgacgccg gcgacggggc cgaggaggac ggcgggcagg gtcgccgcca tcatcacgaa
    11401 gcccatgatc gtgcccgagc ccgtggcctc cttgagccag aggacggcgg cgaggccgaa
    11461 ggcctgcttg ccgagggagc tcacgaactg gccctgccag agcagggtga aatcgcgatt
    11521 ccagagtctc ccggggaggg ctcccgaacc tttggaaacg ctggccgcgg ccccggcggg
    11581 cgccgcatcc gcgcgatccg ggccagccgc gccagaggcg ccggcgcccg cggtctccgc
    11641 ggcgctcgac atgacgtcga gactcttttg tttacgcatc ctggaaatat agctccgcgg
    11701 catcgcggag gataggttgg gcgatattaa atatcaacgt aaatctatct tgacaaaact
    11761 atacaaaagg gacgaaatgg aggcggagga aaccatgcac aagatttcca agggcctcgc
    11821 catcctggcc gccgccctgc tttgctcctg cgccggcgga cccggcggct cgaagcccgc
    11881 ctacgacgcc gacgtgatcg tcgtcggctc gggactcgcc gggcacggcg ccgtctacgg
    11941 cgccctggag ggaggggccc gcgtcctcct cctcgagaag aacgacaggc tcggggggac
    12001 ttccctcacc tcgacgggca ccttcagcgc cgcgggcacg agaatccaga aggagaaggg
    12061 catcgaggac tccgtccagg cccacatcga cgacatcaac cgcatcggcc acggcaaggc
    12121 cgaccaggcc ctcctgcgca aattcgcgga ggaggcggcg ggcgtctggg agtggttcgt
    12181 cgacaagggg atcaacccga accccgcggg ccccgtgatc gaccccgtcc acagccccta
    12241 ctccgtggcg aggacgatga ccccggccaa gaacagcgcg aacgagtatg tccgcgtcct
    12301 ggccgagcag gccaaggcca agtacggcga gcgccttgcg gccctcgtct ccaccaaggt
    12361 cacggccctc gtctccgaga agggcagggt cgtcggcgtc aaggccgcgg gtcccgaggg
    12421 cgagcggatc taccgcgcca aggccgtcgt cctggcgacg ggcggctacg gctcgaaccg
    12481 cgcgatgatc cagaagtaca gcccccgcta cgccgagtac cggacggtca cccctccctg
    12541 ggcgacgggc gagggcatcg agatggccgc cgccgccggc gccgagctcg tcaacatgga
    12601 ctacgtggtc ggctacttcg gcggcatgcc caaggcggcc gaccattacg ccctgggctt
    12661 cggcgacctc acctcgggct tcgccgaccg ctggaagggc gagatctggg tcggatccga
    12721 cggcaagcgc ttcgtcaacg aggacgacgg cgacgaggac ccgcgcgaga cggccatcga
    12781 caagctcgag ggcgggaaga tcctcatcgt cttcgaccag ggcatcctgg acaggaacgg
    12841 cgggaagacc cccatccgcg acttcgacgc ccgcctggcc cagggctacg cggtgaagaa
    12901 ggcggacacg gtcgaggagc tcgcgaaggc cttcggcgtg gacgcgaagg gcctcaaggc
    12961 gacgatcgac ggcgtgagcg ccgcgagcgc ccagggccag gccgactact tcggcaagac
    13021 cacgggcctg cccttcgaga aggggcccta ctacggcgtc ctctgctacg gcacgatctt
    13081 catgacccag ggcggcgtca gggtggacgg ctccctgcgc gccctgggca aggacgggaa
    13141 gccggtcgcc ggcctctacg ccgccggcga ggccatgggc acgagccagt ggggcggccg
    13201 cggctacgcg ggcggcaccg gcgtcgcgcc cgccatcgtc ttcggcctgg acgcgggcaa
    13261 gtccgccgcg gctttcgcga aggccgggaa gtagggcggc atggggcgcc ggccccgcgc
    13321 cggcgcccag gcttccgcgc ggggcgatcc ggctcgcccc gcttcaggcc ttttccccgt
    13381 cgccggaagc ctccggggcg gtgagccgct cgagctcgga gaggtaggcc atcgcctcct
    13441 cgcgggagga gccgcacatc tcgggaaggg gcttgaggga ggcgcagacg gcgggccttt
    13501 cggggcggcc gtagagggcg cagcggaggc tttcgtcgag gaaggggcag gggaggccgg
    13561 ccggcttccc ggccggaagg ccgggaaggg gagaggatat cgagatggcc acgcaacagg
    13621 cggcgcagcc gggcctgcat tcgaggtctt ccaatgtgcc ggcggaggga ctcgaaccct
    13681 catgccatcg ctgacagcgg attttgagtc cgccgcgtct gccattccgc cacgccggcn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cttccgcgcc cgcgtggccc
    13861 gcctcctcgt gctcgcgagc ctgggactgg gcctggtcct cctggtgccc agcctggccg
    13921 acgcctgggc cacggcgcgt tatgcgcttt tcgccgccga ccttgtcgcc tacttcggcc
    13981 tcctctgcct ggccgtcctg gaccggggca ggaggaggtt ctcctcctac gggatagtcc
    14041 tcctggcctt cgcgcagggc ttcttcctct acgcctaccg gggccccgcc ggggcgggcg
    14101 agtcctggtt ctgctccgcc gtcgtcctgg ccgccatcct cttcggcgcc aggggcggcc
    14161 tcgcgctggc gggcatcgtc tccctctcct tcgggggcct gggcctggcc gcggtccggg
    14221 gcctcctccg ctggtccgcc gccgggagct acctcgccat cctggccctc aacaccgtca
    14281 ccctcgccct ggtcgccggc ctggccgcgg cctccctggt gcgctccctg aggaaggccc
    14341 tggaggcggg cgagaggctc agggcggagc tcgccgaggg caaggcggcc ctggagcgcg
    14401 aggccgagga gcgacggggg gccgagaggc tcgcggagtt ctaccgggac cacgacggcc
    14461 gcacggggct cccgaaccgc gagcgcttcc tctacgagct cgggcgcgcg gcccgctccg
    14521 cggagcggcg caagcgcctc ctggcggtgc tggcggtcgg cgtcgacgaa ccgggcaggc
    14581 tctacgacga gtacggacac gccgcctcgg aggcggccct ggccgaggcc gccctcgccc
    14641 tcaaggagtc cttccgcgag gacgacctgg tcgcccgctt cgacgagacc cacctcttcg
    14701 tcctctgcga gaacctcaac gattcggggg acgcgggcga gctcatcagg aaggcgaagg
    14761 gctcctttgc gcggcccttc cgcgccgagg gcgcggcgac cgcggcggcg accgatggcg
    14821 cggtcgcggg accagccggg gccgcggggc cggcgatggc cgcggcgggc gccgggatgt
    14881 ccctcagcgc gagcgtcggc ctggccttct acccgcacga cggcgccggg cccgaggccc
    14941 tcctggaggc ggcgcgggcc gccctccgcc tggccgagga gtcggggccg ggctcctacc
    15001 gcctcttcga cgcccgcctc cacgccggcc tgatcgcgcg gatccgctac ggccgcgagc
    15061 tggaggccgc ggtggaggag ggcctgctcg tcccctggtt ccagccgaag gtggacgcgg
    15121 gcaggaggat cgtcggcgcc gaggccctgg cccgctggcc ccgcgacgac ggcccgacga
    15181 ggatgccgag cgacttcatc ccggccgccg agcgccaggg cgcgatcggg aggctgggcc
    15241 tgcgcatgct caggctgtcc tgcctccggg ccgcggccgt ggagcgcgcg tcgggccgcg
    15301 gcctggtgat ggcggtcaac gtctcgcccc tccagctggc cgacccgggc ttcccggcgg
    15361 agatccgcgc catcctggcg gagaccggcc ttgccccccg cctcctggag ctcgaggtca
    15421 cggagacggg cctggcctcc atggaggggc aggccgccct gcgcctggcc gagctcaagg
    15481 ccctgggcct ccgcctggcc atcgacgact tcgggaccgg cgccagcgcg atagcccgcc
    15541 tcaaggacta tcccgtggac tcggtcaaga tccccaagca gttcgtggac cacctgccgg
    15601 aggagggccg cgccccgacc atcgtgcgcg ccgtcatcga cctggcccac cgcctgggct
    15661 tcgcgacggt cgcggagggg gtggagacgg aggcgcagtt cgagtggctc aagtccgcct
    15721 cctgcgacga gttccagggc ttcctcttct cgcggcccct gcccgccgac gctttcgagg
    15781 ccgagcttgc gcgggccttc ccggaggcgg tagagtaggc cgcatggaaa gcatcctctc
    15841 ctggggcgtg gagctgatcc gcgccgtcca gcgccacgcc agccccgccc tcacgatcgc
    15901 gatgaagggg ctcacggccc tgggctccga gtggttctac ctggccgtgc tgcccgtcgt
    15961 ctattggtgc gtcgaccgga ggaaggggca gaggctcggg gtcgtcttcc tcgcctccgc
    16021 cttcctgaac ctctggctca aggacctctt cgcccagccc aggccctacg acctggaccc
    16081 ttcggtcggc atggcgaggg agtcgagcca cggcctgccc tccggccatt cccagaattc
    16141 cgccgtgttc tggggcctcg ccgctccgca ggtccgccgg ccctggggcc tcgtcctcgc
    16201 gctggccctg cccctcctca tcggcttctc gaggatctac ctgggcgtcc acttccccac
    16261 cgacgtcttc gcgggctggg ccctgggcgg cctcttcctg ggcgcctacg ccctggcggg
    16321 gcaccgcgcg gagcgtgccc tggaccgcct ggacgtacgg gccaaggtgg ccctggtcgc
    16381 gggcctggcc ttcgcgatga acgccctgga gatgcgcgac acctccatcg cgggcggctt
    16441 cttcgggatg ggcatcgggc tggtctacgc cggccgcctg gcgcccttct ccgccggggg
    16501 ccccctgggc aagcgggccc tgcgcttcgt gttcgggatg gccggcgccg ccgtcctcta
    16561 cctgggcctc aaggccgtct tccccggcaa gggctcggag ctctacgccc tcttccgctt
    16621 cctgcgctac ggccttctgg gcgcctgggt ggccctggga gcgccctggg ccttcctcag
    16681 gctcaagctc gcggaacggg aggacctgtc cgcgcgaggc tgagctattc ggccagcctg
    16741 tagttcccgc tcgtgatcct gaagaccttg gtcgcgccgg gcgtgagcca gacgcggcgc
    16801 tccgcgtcga tcatcacgac ctccacgccc gggagtgtag ccgcgagcct caagcccgcc
    16861 tcccgaccca gggcgaagag ggaggtggac aggccgtccg cgtcgatgct cgacgaggag
    16921 attatggaga cggagacgag gccgttctcc acggggtagc ccgtcctggt gtcgaggatg
    16981 tggtggtagc gcttcccgcc ctcctcgaag tagcgctcgt agacgccgga ggtcacgacg
    17041 gtctcgtcct tgcaggtcgc gatgccgatg tagtcgccgc ggtcggaggc ggggtcctgg
    17101 acgccgatgc gccaggggct gccgtccttc ttctggccgt aggcgaagac gttgcctccg
    17161 aggtcgatga tggccgcctt caccttgcgc tcggcgagga cgcgcttggc ctcgtcggcg
    17221 gcgtagccct tcgcgatcgc gcccaggtcg aggcgcatgc ccttctcggc caggaagacc
    17281 gtggaggcgg cctcatcgag gccgaccttc cggtagtcga tgagccgcgt cgcggccttg
    17341 atctccgcgg gagaggggag gcgggccgcg tccgtgccga tcccccagag cttgacgagg
    17401 gggccgaccg tcgggtcgaa ggcgcccccg gtgagccggg cgtactcgag gcccttcttg
    17461 accacgtaga aggtgtcggc ggagacgcgg accggggcga ggccggccgc ggcgttgacc
    17521 gccgcgatct ccgtgccctc cttgttcgcg ctcatcctgt cctcgatccc gcggaggcgg
    17581 gcgaagacgg cgtccacggt cttcgcgtcg ccgcccgaca cgaggcggag ggagcagacg
    17641 gtgccgagga cgaattcggt gcgggcgtcc tccgcggggg cttccttggc gcaggagagg
    17701 aggaggaagg cggccagggc cgcgggcagg gcttgctttc tcatacgggg cgagtatgcc
    17761 tgaaaaggcc gcccttgttc aacgaagggc cgaggggccc gagcccggcc ggtcagccgc
    17821 gctcgcggcc gatggtcgtc ggctcgccgt ggcccgggaa gaccctggtg tcgacgggga
    17881 ggaggaagag ccgctccttg agcgaggcct cgagggcggc gggatccgag tcggggccgt
    17941 ccgtgcggcc gacgccgtcg cggaagaggg tgtcgccgga gacgaggacg ccccgcgcct
    18001 cgtcgtagag gcagatggag ccggcggtgt ggccgggcgt gtggatgacc cgccaggtcg
    18061 agccggggag gagggccccg tcctccagga ggaggccggc ctcgggcatg gttttccagt
    18121 agctcttgaa gtagcccatg gccctgatgg cctggaagac gcggcggttc gtctcctcgc
    18181 cccgctcgcc gaagtaggcg gcgtccgcgc ggtggacggc gaggaggagg gcccggccgc
    18241 gggcggcgaa ggcggccatg agggagggaa gggcgccgac gtggtccagg tggccgtggg
    18301 tcgcgacgac gaggctcggg acgaggcggc gggagtcgag gaagtcgagg atgcgctccg
    18361 cctcgtcgcc cgggtcgacg aggacgcaag gcccgccctc gcggggcagg cccgcgccgc
    18421 cgtcaagcgg aaggacgtag gcgttctcgc cgatgggacc taccgtcagg cgctctatca
    18481 tggtatcgtg aatctagcct aggcacgcaa gtaagggcaa gccttgttca taccgctcta
    18541 cgcggtcctc gcggccgccc tggtcttcta cctcctcctc cccgaggcgg ccgtcctctc
    18601 ccgccgcctc tcctggcgac ggttccggcg caggctcgag gccctgtccc gggctcccct
    18661 cctccgctac cgcgacgccg tcccgccccg cgccctcctc gaaggccaag ccggcgcggc
    18721 gggccaggcc ggagccgcgc cggagggccg cgccgccgcg cgcggggagg gcggcgcggg
    18781 agatggcggc aggggcgagg cgcgcggccc cttccgcctc tacggccgta tcgaggcgat
    18841 ggagggcgac gaccgcgtct ggctgcgcgg caaggaggtc tccgccctgg tggacctgtc
    18901 gcgctcgccg ctctacgtgc tctcccccgg ccccgaggag gaggcgggcg cggggagcgg
    18961 cgagcggctg gagcggatct cctggcggcg cgtgcgcgtc ctctccgagg ggacgaggct
    19021 catggccgct gggcccctcc gcttcgaggg cggcaggccc gtcttcgccg aggccccggg
    19081 ggagcccctg gtcgccgtga tccacggcgg cggggagggc gatctcctct cccgcctcgt
    19141 cgccggcgcg cgccggcccg ccctggccgg cggccccttc gccacggtct ccgtcgcctt
    19201 cggcctggcc ctgtcgagcg ccctcctctt cctcctcgcg gagggacgcg gcgtcctccc
    19261 gaccgtgcgc gtcctcgcct acctggccgc cctctctccc ctcttgccgt ttctcccgcc
    19321 gggcctcgcc ctgtatctcc tgtaccgccg cctggccagg gaggcccacg ggctccgcgt
    19381 ggccagggac ctcctgcgcc tgcccctgcg cttcttcgga agccccgacc tggccgaggc
    19441 gaggctggcc tgcggctcgc cttaccggcg gagcctgggc ggcccggcct cggggccgcc
    19501 gccccccggc tacggccccg tcgcccttcc cctcaagccc ggggccggcg cctcctggac
    19561 cctcttcggc cccgaggagg gctgcgagga tccggcggcc gagcgggtgg ccgtccccgg
    19621 cgagcccgag ctcctggccg agcgctgcgc cgggaaggcc cggcgcctgg gcctgggcgc
    19681 ggtcctggcc ctggcggcgg gcggagccct gaacttcggc ctcgtcttcg cggtctggcg
    19741 ctccctgccc tagggggccg cgcgctaaaa gaaaaggccc gcggaaccgc gggcctttcg
    19801 tggcgaaaac ggggcaggct aaagggcctt gtcctcgtcc ttctcctcat cgaggtcgtc
    19861 ctcgggctcg tcggcaggct cctcgtcgta ggaggcgtcg gagccatcgt cctcggcgag
    19921 atcctcgccg aggggagcct cgtcgccgta ggcctcgtcg tccatggacg cgtcctccag
    19981 ggactcgtcg aagccggcct ccacggcgag ggcctcgccc tcgtcgcggc gaccgccttc
    20041 cccgcgatcc tcgcgaggct ccggggcggc ctcgccgcgc ttgcgcgcct tgttgacggg
    20101 aaggacgcgg ccgcggaact cgaaggaggc gagggcggcg atcagctcct cggcccgctc
    20161 cggcgccacg tcggcgaagg agtagttgtc gaaggagcgg atgtccccga tgtcctcgag
    20221 ggcgaggccg ccctcttcgg agaggacgcg gaggaggacg cgggaataga ggcgctggcg
    20281 cttgcccatc ccgaagaaga tcgtggtccc ctcgccctgg aggcgggggc gctgctcttc
    20341 cggcgcggcc tcgcggggct cgggggcgcg ctcgcggcgg gcgccgcggc cctcgccgcg
    20401 cgattcgccg cgctgggact caggccttgg ctcgtcgccg cgcgaggact ccccgcgggc
    20461 ggactcgccc ttgccgcggc gcttgccgcc ggccttgggc tcgtcccggc ggcggtcgcc
    20521 gcgctgggct ccctggcggg acgcctcgag gaggagggcg gcggcggcgt aggagcggag
    20581 atggaggggg acctttttcc tgaatgcggc gcgcacttcg ttcagggctt cgggatcgga
    20641 gggcgagctg agctcggcga gaatccggtc gatgtgagcg agggcggccc ctgcgggcgc
    20701 ttctgccgca ggacggggct tcggcacttc ggactccttg atgcggtgct gttctcaggc
    20761 gattctctcc taaaacagcg cgctccgcaa ggcgcttccg gggctttttg aggcgcgcgc
    20821 cgaggcggcc ggcgaggggg ccgcgtccag gccgcgtcca ggccgcggca aggccgcggc
    20881 aaggccgagg cctgcatggc cgccggcctg gtccggctgg ccgagggccc catggagctt
    20941 gtccgcgccc cgcctatccg ctacagtagc gggagcatgc tcccgcagaa acttcccctc
    21001 gtgcccgccg ggctcctctg cctcctcctt ctcgcgccgc ccttggaggc ggaggcctcc
    21061 tccggccccg ccggtccggc cgcggccaac gcctccgcgg ctccagccgc gacgggcctg
    21121 ggctccccgg gtccgtcggc cgccgcccag tcgggcggag cggcgccggg cgcctcggcc
    21181 tccctcccct cctgccccat cctccccgac gaccccgcgc ccctcttggg ccttagcctg
    21241 gccgagtcct acctccgcct gggagcgccg gcctccgtgg ccgccttccg cggcgtggag
    21301 gcctggcagg acgacgtggt cttcagcttc ggctcgggct actccctctt ctggttcggc
    21361 gaccgcctct ggcagatccg cttcacgccc ggctacggag gctccgtcta cggcctcttc
    21421 ctgggcgacg gcaaggacaa ggtctactcc ctcctgggcg cgcctttcta cgaggccgag
    21481 gggagcctgg tctaccgcct gccctaccgc ggctacccgg tccgcctccg cgtcgtcgtc
    21541 gccgagggac gcgtggccga cctctatctc taccgggccg atttctagga ggagccttgt
    21601 ccctcgtctg cctgtccctg accggaaaga gcctcgccga ggacctggcc gtgctggacc
    21661 tctaccgcgg ccaggtggac ctggtcgagc tcagggccga ctacctggat ccgagcgaga
    21721 agttcctgat ccgctccttt cccgagcggg ccggcctgcc ctgcatcctc acggtcaggc
    21781 ggcgcgtgga cggcggggcc ttcgccgagg gcgagggcgt gcgcctcgtg atgatcgcga
    21841 agggcctggc ctacgcgcgg tccgagcggc tcgccaactt cgcctacgtg gacctggagt
    21901 ccgacttccg catccccgcc gtggaggagg cctgccgggc cttcggcacg aggatcatcc
    21961 gctccgtcca ctcgcccaag agcctgcccg ccgacctgga cgccctctgg gacgagctgg
    22021 ccgagacccc cgacgagata cccaagctcg ccctggcctg cgagagcgcc tccgacctgt
    22081 cccgcctctt cttctgggcg gagagcctgc ctccgcgcga gcgcatcgtc gccgggatgg
    22141 gcgactacgg cttcccctcc cgcatcctgg ccgagcgcac gggctccctc ctcacctacg
    22201 cgagcgccct ggaggcgggc ctgccccacg cggcccccgg ccacctggac ccggaggccc
    22261 tggcgagcac ctaccgcttc cgagagatcg gcaaggccac ggccgtctac gccctcacgg
    22321 gcggacggag cgtcgtgggc gcccgctcgc ccgccctcca caacgcggcc ttccgcgcgg
    22381 ccggcatgga cgccgtctac ctgcccttcc ccgcggagga cgccgccgcc ttcctggccg
    22441 cggcggaggc cgtgggcgtc cggggcgccg ccgtcaccgt gccgcacaag gaggccttcg
    22501 cgcccctcat ggcgcggctc tcccccgagg cggcggagat cggcgcctgc aacaccctct
    22561 tccgcggccc cgaaggctgg gagggcagca acacggacgc gcccggcttc gagagggccc
    22621 tgcacgaatt cctgggccgc accgaccttt cgggcctcag ggcgaccctg gtgggcgcgg
    22681 gcggcgcggc gaaggccgtg gcccaggtct tcgcgaagtc cggcgtctcc tgcctggtcc
    22741 tgaaccgcag cgccgccaag gccaaggccc tggccaaggc gcgcggcttc gcctgggcgg
    22801 cgaacgacga gagggctgcc gagctcgtcg ccgaccacgc cgacctcatc gtcaacgcga
    22861 cgagcgtcgg catggacggc gggccgccgg gcgaccccct ggaatggtac gacttcacgg
    22921 gccgcgaggc cgtcttcgac ctcgtctacc gccccgaccg caccgcgttc ctcaagcgcg
    22981 cgaagacctc gggcgccagg atctccaacg gctggaccat gctgcgctac caggccgccg
    23041 agcagttccg catctggacc ggcctagagc cgccccgaac ctatttcgag tgaggtaacc
    23101 catggccaag tcagcattcg tcgcgatcgc cggacgcccc tccgcgggca agtcgaccct
    23161 cgtcaacgcc ctctgcggcg gcaaggtctc catcgtgagc cccgtgcccc agacgacccg
    23221 caacgcggtg cgcggcatcg tcaacaagcc cgagggccag ctcgtcctcc tggacacgcc
    23281 gggcttccac ctctcggaca agcgcctcaa caagatgctc aagaacgtcg cc
//
LOCUS       DAOOSJ010000011        22142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1612_length_22142_cov_6.451578, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000011 DAOOSJ010000000
VERSION     DAOOSJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22142
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_22142_cov_6.451578"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PLG14_01280"
     CDS             complement(<1..1577)
                     /locus_tag="PLG14_01280"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX47787.1"
                     /translation="MRIAPRIVLLFLPVVVVPMAFQTLLAAGSAREGIASIAADHLRF
                     KTEELLRFADSQAGLLEANGLSGKPEFQAAAVATIASYAEGLARAPGERIAAVDAAGR
                     LVLRAGDGEWTPEDSEALAALAAGGEGWRELDLPGGKRVSFVAAFPPFGWTIAVTVLE
                     DRFFGPVSRIAFQGVLVGAVGAAAAALLLAAFAGLITEPIRRVAKGMRAVVETGDLSR
                     RVSLPYDDEIGDLGDSFDAMAGSLEAAYAEIKGYALDAAIAHKREARIRNIFQKFVPA
                     PVIERFFASPESMLVGEDRQLAVLFSDIRGFTAYSETLSSREVVATLNRYFGRMADAI
                     VRHEGIVDKYVGDELMAFFGAPAPDARSAYHAVAAAFDMIAELDDFNAFERERGRRPL
                     SFGVGINYGAVTIGNIGSEKKMDYTVIGDMVNLASRLEGLTKYYREPLIVSDSVRGLA
                     GADFPMRLIDRTRVKGREGGLSIYAARLSLSAEEERAWAIHAEALELYYARRFAEAAA
                     RFGEVLRLLPDDPVTLI"
     gene            1782..>2874
                     /locus_tag="PLG14_01285"
     CDS             1782..>2874
                     /locus_tag="PLG14_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47788.1"
                     /translation="MDRAQKARERAAALEGADGVTREDRARILADIEALFLAKRALPP
                     RPKAARKRGLLLPIAANAAAALLLVLGLLLFPPGRSAGYAGARRAGAIGLKVEESLIG
                     RLREESRLALSDRERRIAEIRAELERLRLAPRPAAGGAAAASRAASAEEAAAREAAES
                     ARLELERGLRAELAALETESSRRLAALEKERAEAAFLLGQLRGVYAEARRLAASGDRE
                     AARSRAAAAGTLLDRIGASGPGGPELAALLREAYAAVDEALLAAARAEAQAEADAQAA
                     ARLAGVQAAGGAAGAQAEELSKAREAQEAAESRAALAAAAADRAQARVLELERSLAAT
                     RLGLEGELGGAKEELGGAKAALAAANEELA"
     assembly_gap    2875..2974
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2975..3481
                     /locus_tag="PLG14_01290"
     CDS             <2975..3481
                     /locus_tag="PLG14_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47789.1"
                     /translation="ELEAARLALAEGEARKAERWERSDRLAHELSAELASARPLLPPA
                     AAPTEADIIALLEAKLRLRELAGSQARERGDPAIYDDFDRSLERIALEERRAGEAEAY
                     AKASAALAGLEARLGLEAAADPSADGKPPAAVQPAPAAGPAAAQAPAEDYLGRLDRLL
                     AALVERLR"
     gene            complement(3478..4776)
                     /locus_tag="PLG14_01295"
     CDS             complement(3478..4776)
                     /locus_tag="PLG14_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOX47790.1"
                     /translation="MLRRVMLRVLVVLAALTWACGDFVLYDAFLEPLSLSPSSAYLMV
                     AESRSFEASGGTPGYRYRLRSGGGSIDPATGLFTAPATAGECLVEVVDSGGRTASALV
                     SVFESHALAIVPAAAALQIGAALQFEGAGGEPPYSFVLYSGVGSVSPAGLYLASAEGD
                     AVVKISDGEGSVATAFVSVLAPAALTITPLSTTITAASTFQFVVLGGTPPYAFAVTAG
                     LGSVNATGLYSAPPGATTATLRASDSASPPAVAVATVTVVSGGALGLSSSSTAVEEGA
                     TASFQAYGGTPPYAYSLAVSGSGGSVDAGTGLYAAGHAVGAAKDRVRVTDAAAATASL
                     DVDVLPAAPSGLVADGSFGGPKEIRLTWTDHSSSETGFSIERKEGTGAYAFLALTAAG
                     VTTYDDANLVPNTGYSYRLRSFSGALYSPYSNEAFDVPNN"
     gene            complement(4786..5871)
                     /locus_tag="PLG14_01300"
     CDS             complement(4786..5871)
                     /locus_tag="PLG14_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47791.1"
                     /translation="MKPGRILLVLAAMVLPLASHAQTRILIESSGGAEARGLAGIVER
                     CAVLALERAGFAPAQGSGAGEDPPEALLLCSSSLAAGGYELSLELRSAAEGQALLAEA
                     RLSGPLTLDLDERLIEEVSALVALSGLEPGPPPAPRPAVASLPGDAAPRAGAAAPASA
                     SAEAPVLARAPAGGEAVLMTGPVPISAGGEALVIPQSGGPPALSRLGLGLSTAPVLVV
                     GAASDYFRFGFDASCSLGYSFRFGSFSLEAGLRAGYALLYSAGQVGGEVHLASLGPEF
                     RGRWPAEGRLSAFLRAGLGPLYLAVAPETGETLGKILPACSAGLGLEFLPSPGFSLGL
                     EVGLLAVFERNLPLLGLAPGLVAAWRL"
     gene            complement(6042..>6308)
                     /locus_tag="PLG14_01305"
     CDS             complement(6042..>6308)
                     /locus_tag="PLG14_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47792.1"
                     /translation="PGAAGAAAGRLLGYAVASPSGTIWQLAVAREARRRGIGTALLRA
                     LAAKTGGALRYINAQSDDAGTRTLLARSGIREDVGQYEMTLELE"
     assembly_gap    6309..6408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6409..6944)
                     /locus_tag="PLG14_01310"
     CDS             complement(<6409..6944)
                     /locus_tag="PLG14_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47793.1"
                     /translation="MFEFRTLDSEAPARRYAAFMEAFSDYSVPVAWSEAEFDAVNARR
                     GADWSLSLGAYEGGRLVGFVMSGSGEWGGRKAAYDMGTGVVPSARGSGLAGLLAERLK
                     GLLAERGVGLYLLEVIRDNAPALKTYERAGFRKTRDFECSEGTFVDPGKAPPEGVAVG
                     ELAAFPRAEAAAMRDWEPS"
     gene            7047..8300
                     /locus_tag="PLG14_01315"
     CDS             7047..8300
                     /locus_tag="PLG14_01315"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOX47794.1"
                     /translation="MKEAGAARRILVLNSGSSSLKYEVFLMPNRESLGKGSVERIGEA
                     KGILTQTSPKGQIRLDEPIKDHGEAMARVAKALVDPKAGILGSIGEIEAIGHRVVHGG
                     ERFASSVVIDGPVLEAIEANVELAPLHNPANLTGIREAERLLPGIPQVAVFDTAFHQT
                     MPPSAYLYGLPRELYDKYKIRRYGFHGTSHRYVAGRALELLKRHPDNTNLITCHLGNG
                     ASITAIEQGRSIDTSMGFTPLEGLMMGTRSGDIDPAILGFLEERGYTAKDLSNLINKK
                     SGLLGLSGISNDLRDLEAAVDAKDKNATEALEVYAHRVRKYIGAYSAELFRVDAIVFT
                     GGVGQHGWRMRERICHRLENIGIIMDYERNRTDGSAEGVVSAPYSPTAILVVPTNEEL
                     QIACDAFELLFAPEELMRRKSDVQV"
     gene            8359..8961
                     /locus_tag="PLG14_01320"
     CDS             8359..8961
                     /locus_tag="PLG14_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX47795.1"
                     /translation="MPREKAFLDTRVIIDKTIELIEAEGYERFSTRRLAAALGSSVMT
                     LYNYYENREAILREALLGCFENLLAGIEARLEPYLSGERGSPLRAYVALGELLRAFSL
                     ERPRIYAFLFEANLVPLRTDPRIAAVYGYTYGRIEALLRDPARSGELHDRIYLFQVLM
                     NSLVKNAARGRGGIDPARFEALLAEAYSALLAPYEGLVAP"
     gene            complement(8937..9446)
                     /locus_tag="PLG14_01325"
     CDS             complement(8937..9446)
                     /locus_tag="PLG14_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOX47796.1"
                     /translation="MPDGGTMDGSRCDSYCGIYCGACDILAACRRGEPGAFSAYLKER
                     VPPAGDWRCKGCKSDEVFGNCARCPIRACARERGLESCAACPEFPCAAYSRPDAERLL
                     AELPHLKLNAGNLAAIAERGVEAWLRGQEELWRCPECGAPDSWYAETCPVCGASLAGR
                     KPYGATSPS"
     gene            complement(9458..10219)
                     /locus_tag="PLG14_01330"
     CDS             complement(9458..10219)
                     /locus_tag="PLG14_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HOX47797.1"
                     /translation="MSELNLSGASVDDLARGFARDEAGPGFRCLACGALFEDGRAYQE
                     GEAFLLPERAARAHVQRAHGGAFALLRGLGDEAAGLPEVQGRVLGLLHEGRSDEEIAA
                     LLGGKSPSTVRNHRFNLRKREAEARVFLALMRLLDEGGSGAGRFLDYPAGMPAKDERA
                     AVTEAEAAAIEARHLRDLPGGGFAFASWPKRQKEKLVLLRRAAGLFEAGRRYSEPEVN
                     AILSPLWDDHVTIRRYLIEYRFLARKPDGSEYWVA"
     gene            10352..11011
                     /locus_tag="PLG14_01335"
     CDS             10352..11011
                     /locus_tag="PLG14_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX47798.1"
                     /translation="MPRNLTFNAESVLDAALDLARKEGIEALSARAVAARLGASTAPV
                     YSAFSSMESLREALLDEALELLDASVEHRYTEGRFLNQGVGFAVFARDEPRLFFALFD
                     AGEGDLLERYRASLVDRLFDDPALGRLERPAIERIAGRMWTYTVGLAFYLAHGRRADA
                     STGALIEELRSAGSVAIYGELKGVADYGSAEAREAWAELVARKGLDLELKPLAMRSEV
                     N"
     gene            11046..11399
                     /locus_tag="PLG14_01340"
     CDS             11046..11399
                     /locus_tag="PLG14_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47799.1"
                     /translation="MSARNPRAYGALLGLSGLFALGAVLTLLPNPGASWENVLGYRSL
                     CAFAPIATALCALLAGATCVLRARLFGPRAGDRRSWAAPVAVGLVLLAVIAFSVPPYA
                     RAKAEAVSGASALAE"
     gene            complement(11495..12094)
                     /locus_tag="PLG14_01345"
     CDS             complement(11495..12094)
                     /locus_tag="PLG14_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47800.1"
                     /translation="MNRPHPGMRHNAVRASTARLLSYVRGLGWALVLLLVAGGGLALV
                     QAYFHPFDIVIEKADESLAAIDTTFSANAVAGVSIGTLVLIAAAIALPLMRKGVRVRQ
                     YFVSFWRGLVSAAIFLATDKLYRYVQSLGVLYFSATLALFLAATIVLVEIVARAGRIE
                     EEAERRTEFLASIVSGLVFGLLVQLGEYLFGLLTKALGL"
     gene            12200..12934
                     /locus_tag="PLG14_01350"
     CDS             12200..12934
                     /locus_tag="PLG14_01350"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOX47801.1"
                     /translation="MRDSEKAASPGLGGLAEALREALSRPLPGRSAQDLMLPAYRLGL
                     PEEPEGRDGWRQAAVLVLLYPGGGPPAGAGSSPGAGRLAAADPLAGAGPLLFPLIERS
                     TEVGRHRGEVGLPGGALEADETGEEAAFREAREELGLEAEAARPALLGALSPLRVPPS
                     GYEVRPYVALLPRRPAFRPEPAEVAELFEAPLSRLLEPEARGEEERLFGGRPFLVPFF
                     GLGGRKVWGATAMILAELSAVLESLR"
     gene            13074..14228
                     /locus_tag="PLG14_01355"
     CDS             13074..14228
                     /locus_tag="PLG14_01355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOX47802.1"
                     /translation="MSELPPFEEMDKAFRSRDEAYDGLFYAAVSTTGIFCRPSCPARK
                     PLPGNVRYYATAAEALFAGYRPCKRCSPLSGSGDPPWLGALVERVEADPRARIRDAEL
                     RAEGLDPATVRRRFQARFGLSFQAYQRARRLAAAFEAIKEGGSIDDAVFDSGYDSHSG
                     FREAFARLFGAPPGAAKAGGAAKAGGGARAGASGTAGAAAIPADTDAAAGKLADFIRL
                     SWIDTPLGPMVAAATDRGVCLLEFSDRRMLEAQAKILSARIGLPALPGAHPHLERLAA
                     ELAEYFAGKRRSFEVPVFEPGTPFQERVWAALREIPWGQTRSYAELARAIGDPAATRA
                     VARANGMNRVAILVPCHRVIGADGGLGGYGGGVWRKKRLLEVEGAASPGR"
     gene            14458..14925
                     /locus_tag="PLG14_01360"
     CDS             14458..14925
                     /locus_tag="PLG14_01360"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOX47803.1"
                     /translation="MDKSKTIALVAHDNMKRALIEWVEYNWHTLSKHRLVCTGTTGRL
                     VEEAIAAKNGGVLPHGFSLTRLKSGPLGGDQQLGALIAEGKIDAVVFFWDPMSPQPHD
                     VDVKALLRIAVLYDVPMACNRSSADFLISSPLMGREYHPTPLDLSGYVNRKIG"
     gene            complement(14951..16027)
                     /locus_tag="PLG14_01365"
     CDS             complement(14951..16027)
                     /locus_tag="PLG14_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX47804.1"
                     /translation="MPLPAQAPLDILGLTRAGFEAAAGLGAGRSRALGALYKEAFATG
                     RFGPESTGISAEAAASLASRFRVGLLKPLRVVEEEGPLGVTAKALLELGDGGTVECVR
                     IPVASGGAATLCLSTQVGCRMGCAFCETGKRGLSRDLRAAEIVAELVTARVLLGWNIR
                     NLVFMGMGEPLDNFEELAAALAVLLDPAGAAFSQERVTVCTVGLPEGIARLKALGLKR
                     LNLSVSLNAPDDGIRSALMPANRGAGLDRLAEALAAYPQRRNFALAVNYCLIPGLNDG
                     PGAPEGVAAFLAKAAQPGRALVNLIPYNPGSEPLGPAPTEAEVEAFEARLRALGVQVK
                     LRAPRGRGLMAACGQLGGAEGGGE"
     gene            16203..17036
                     /locus_tag="PLG14_01370"
     CDS             16203..17036
                     /locus_tag="PLG14_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47805.1"
                     /translation="MAAKKKAAGDRLFMDNLAQAVREEYVKVAVEEARARRPLPALAA
                     PEAPKTTGAAAKAAGTKAAPAASARKPAASEKPKAAEAKPAIAKSAAKPVAKAAGKTA
                     SAKTTGKANSAKASKAEKAEAEEKALLLKDLHALLPRLDAEGLAFLLEQAEVHLYNME
                     AEALEASRAERQRRAAERRGVAPGAAGGLALVRSESGSSYYVEAGDASVMFTKEEMLS
                     LVRLAHGHPDDLEAARALGAWLERERRDALDELRLPGAQGPALKELARLLRSSFKRPG
                     A"
     gene            complement(17173..>17971)
                     /locus_tag="PLG14_01375"
     CDS             complement(17173..>17971)
                     /locus_tag="PLG14_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOX47806.1"
                     /translation="AAPGAPASPPPLPEMVRAEAGAFVMGSEDGAADERPAHRVRIAR
                     DLLVAVRKVTAEEYDRFCDETGRARPKSRLGFDGSRLPAIGVDWYDAVAYCDWLSRLH
                     GLAPCYSGEGLKTACDFGASGYRLPTEAEWEWAARGGRLSKGFRWAGSDDPLAVAWFG
                     ENSQGAMREVGLLGPNELGLYDMSGNLYEWCWDFYGRDYYAASPEIDPEGPPPGRPAT
                     PRGPEHSRRAGSWREGRESVRVAFRSLDNAGYPGDNGFRLFRTAPSE"
     assembly_gap    17972..18071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18332..19276)
                     /locus_tag="PLG14_01380"
     CDS             complement(18332..19276)
                     /locus_tag="PLG14_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX47807.1"
                     /translation="MQHLALAATLAAFGTGLAVLGLFLGLYRAGRSPLVRSYLVYLAC
                     GLFQLLAGLAGLYASAAGLAPDPDALSRAYYFSEVLASGILAYAAPRFFLEFADLPFR
                     GGPRRAAIGLAAATLASSPLALVEGGAPFLRFVPPSLGLLSFLGLMLYSQYRLIRAYR
                     GVEDRLGRIGIPAILAYNAFCLAAGTIDSGLSSAQLAAGAWPYGALLRPATYLAWYLL
                     SLAWAARYEASGALASGASGPTDRERALALGLTGREFELASLLSTGAANKEIAAALGL
                     SANTVRNHVHNMFEKTGARNRVELVRLLSGDAASAGRG"
     gene            19450..20391
                     /locus_tag="PLG14_01385"
     CDS             19450..20391
                     /locus_tag="PLG14_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX47808.1"
                     /translation="MEGKLGAAVEARGLEKSFGSRRALRGLDLSIPRGGVFGILGPNG
                     AGKTTTVRILAALARPDSGSVEVLGRDARKEARAVRALVGLSPQENAVAGRLSAMENL
                     LLVAGSYGLRGRDAKARAEACLRALGLEDRARDRASALSGGLTRRLSIAMALVADPPL
                     LFLDEPSLGLAPEARAELWRVIASTKGEKTVILTTHYLEEAEALADRVAVVVDGKVVL
                     EDSPAGMRRRAGGGGRVRLELSGEEEAARLAAALAGAFPGLRASGARLELEGGEAAGL
                     ALLDRARAAGAVPRGFSVGEASIGEAYLAAIGKGGEA"
     gene            20388..21116
                     /locus_tag="PLG14_01390"
     CDS             20388..21116
                     /locus_tag="PLG14_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX47809.1"
                     /translation="MRWLSLARRNLAEILRDPLSAGLGAAMPCALLTLFAALGRSVPA
                     PLFRIGPMTPAYAVFSFAMLVMFSAMLLSRDRSGGLFGRLLATPLEPRDFALAYALPF
                     LPVAALQAAACYAVGAAFGAPLGAGAAASAAALLPAALGFVGLGLCLGALCTENQIAG
                     LGSALVTAVGVFGGLYFDPAAAGGFFAAIGRILPFASAVEAARLLFAGSCLAAAAAPL
                     FKTWAFAALALGAGVLAIRARARR"
     gene            complement(21223..21693)
                     /locus_tag="PLG14_01395"
     CDS             complement(21223..21693)
                     /locus_tag="PLG14_01395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47810.1"
                     /translation="MTRPDVTSPGAIRPWREEDLPRIAALVRELDEALGGDFRPDEGR
                     LRAMFGRMAALPGIYETYVYEEEGEVLGFVSLLFYESAYHRVGTAQVNELVVDARRRS
                     GGVGAALLARAVARARARGMDEIEVGVERENEGARRFYARHGISEEYLLLGMEL"
     gene            complement(21690..>22142)
                     /locus_tag="PLG14_01400"
     CDS             complement(21690..>22142)
                     /locus_tag="PLG14_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47811.1"
                     /translation="LEGSELGRRYFCDARGRYIGGELLREGFEKEEVSVALGGDGACL
                     GFAWIQPRGIFHWFPFLHVVAVRADLRGRGIGGKLMDLFERAAFVEERSPMAFLMVAD
                     FNAEAIALYGKRGYERVGAVPGLFLPGVEEILMMKRAPVPGGAAGAPA"
BASE COUNT         3098 a         7932 c         8107 g         2705 t
ORIGIN      
        1 aagatgaggg tgaccgggtc gtcggggagg aggcgcagga cctcgccgaa gcgcgccgcc
       61 gcctcggcga agcgcctcgc gtagtagagc tccagggcct ccgcgtggat cgcccaggcc
      121 ctctcctcct cggccgagag cgagagcctc gcggcgtaga tcgaaaggcc gccctcgcgg
      181 cccttcaccc gcgtgcggtc gatgagcctc atggggaagt cggcccccgc gaggccccgc
      241 acggagtcgg agacgatgag gggctcgcgg tagtacttcg tgaggccctc cagcctggag
      301 gccaggttca ccatgtcgcc gatgaccgtg tagtccatct tcttctcgga gccgatgttc
      361 ccgatcgtca ccgccccgta gttgatgccg acgccgaagg agaggggccg cctcccccgc
      421 tcccgctcga aggcgttgaa gtcgtccagc tccgcgatca tgtcgaaggc ggcggccacg
      481 gcgtggtagg cgctccgcgc gtcgggggcc ggcgcgccga agaaggccat gagctcgtcc
      541 cccacgtact tgtccacgat gccctcgtgc cggacgatcg cgtccgccat cctcccgaag
      601 tagcggttca gcgtggcgac cacctcgcgc gagctcaggg tctccgagta cgcggtgaag
      661 cccctgatgt cggagaagag cacggcgagc tggcggtcct cgccgacgag catggactcg
      721 ggcgaggcga agaagcgctc gatgacgggc gcgggcacga acttctggaa aatgttgcgg
      781 atgcgcgcct cgcgcttgtg cgcgatcgcg gcgtccaggg cgtagccctt gatctccgcg
      841 taggcggcct ccagggagcc ggccatcgcg tcgaaggagt cgcccaggtc gccgatctcg
      901 tcgtcgtagg gcaggctcac gcggcgggag aggtcgcccg tctccacgac ggcccgcatc
      961 cccttcgcca cccgcctgat cggctccgtg atgagcccgg cgaaggccgc gaggaggagg
     1021 gcggcggcgg cggcgccgac cgcgccgacc agcacgccct ggaacgcgat gcgcgacacg
     1081 gggccgaaga agcgatcctc caggacggtg acggcgatgg tccagccgaa gggcgggaag
     1141 gcggcgacga aggagacccg cttcccgccg ggcaggtcga gctcccgcca gccctcgccg
     1201 cccgccgcga gcgccgccag ggcctccgag tcctcaggcg tccactcgcc gtcccccgcc
     1261 cgcaggacga gccggcccgc ggcgtcgacg gcggctatcc tctcccccgg cgccctcgcc
     1321 agcccctccg cgtaggaggc gatggtcgcg accgccgccg cctggaactc gggcttgccg
     1381 gagaggccgt tcgcctccag gagcccggcc tgcgagtcgg cgaagcgaag cagctcctcg
     1441 gtcttgaagc gcaggtggtc ggccgctatg gacgcgatgc cctccctggc cgagcccgcg
     1501 gcgagcaggg tctggaaggc catcggcacg acgacgaccg ggaggaagag gaggacgatg
     1561 cgcggcgcta tcctcacgcc gacaagcata gtccaaagcc cgcgccggga caaatcggcc
     1621 gcctgaggcc cggaggccga cgcccgcggg acggccgccg ccgactcatc cccgcggggc
     1681 ggccgccgag gcttcgccgc cgcgcgcgac gcgccgccgt ttgcccccgg cccgcccatg
     1741 cgctagagtt gaatggcgga ggtaggacag tggggggcct cttggacagg gcccagaagg
     1801 cccgggagcg ggcggccgcc ctcgaaggcg cggacggcgt cacgagggag gacagggcca
     1861 ggatcctcgc cgacatcgag gccctcttcc tcgcgaagcg ggccctccct ccgcgtccca
     1921 aggccgcgcg caagcgcggg ctgctcctgc cgatcgcggc caacgcggcc gcggcactcc
     1981 tcctcgtcct gggcctcctc ctcttccccc cgggccggag cgcgggctac gcgggggcca
     2041 ggcgggccgg ggcgatcggg ctcaaggtgg aggagagcct catcgggcga ctccgcgagg
     2101 agtccaggct ggccctctcc gacagggagc ggcgcatcgc ggagatccgc gccgagctcg
     2161 agcgcctccg cctggccccg cgccccgccg cgggcggagc ggccgccgcc tcgcgggccg
     2221 cgtccgcgga ggaggcggcg gcccgggaag ccgccgagtc ggcgcgcctg gagctggagc
     2281 ggggcctcag ggccgagctc gccgccctgg agaccgagtc ctcgcggcgc ctggccgccc
     2341 tggagaagga gcgcgccgag gccgccttcc tccttggcca gctccgcggg gtctacgccg
     2401 aggcgcggcg cctcgcggcc tccggcgaca gggaagcggc ccgttcccgt gccgccgcgg
     2461 cggggaccct cctcgaccgg atcggcgcct cgggaccggg cggacccgag ctcgccgccc
     2521 tcctccgcga ggcctacgcc gccgtcgacg aagccctcct cgcggccgct cgggccgaag
     2581 cccaggccga agccgatgcc caggcggcgg cccggctcgc cggggtccag gccgcgggcg
     2641 gagcggccgg cgcccaggcg gaggagctgt cgaaggcccg cgaggctcag gaggcggccg
     2701 agtccagggc ggccctcgcg gccgctgccg cggacagggc ccaggcccgc gtcctcgagc
     2761 tcgagcggag cctcgccgcg acgcggctcg ggctcgaggg cgagctcggc ggggcgaagg
     2821 aagagctcgg cggcgccaag gccgccctcg ccgcggcgaa cgaggagctg gccgnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagctc gaggccgccc gcctcgccct
     3001 cgccgaaggc gaagcgcgga aggccgagcg ctgggagcgg agcgacaggc tcgcccatga
     3061 actctccgcg gagctggcct cggcccgccc cctcctgccc ccggcggccg cgcccacgga
     3121 ggctgacatc atcgccctcc tcgaggccaa gctcaggctc cgcgagctcg ccggctccca
     3181 ggcccgcgag cgcggcgatc ccgcgatcta cgacgacttc gaccggagcc tcgagcggat
     3241 cgcccttgag gagcgccgcg ccggcgaggc cgaggcctac gccaaggcct ccgcggccct
     3301 ggccggcctg gaggcccgcc tcggcctcga agccgcggcc gatccgtccg ccgacgggaa
     3361 gccgccggca gccgtccagc ccgcgcccgc cgccgggccg gccgccgcgc aggcgccggc
     3421 cgaggactac ctcggccggc tggaccgcct cctcgccgcc ctggtggagc gcctgcgcta
     3481 gttgttcggc acgtcgaagg cctcgttcga gtagggcgag tacagcgccc cggagaagga
     3541 ccgcaggcga tagctgtagc ccgtgttcgg gacgaggttc gcgtcgtcat aggtggtcac
     3601 gccggctgct gtcaaggcga ggaaggcgta agcgcccgtt ccctccttcc gctcgatgct
     3661 gaagcccgtc tcggaggagg agtggtccgt ccaggtgagg cggatctcct tgggccctcc
     3721 gaagctgccg tcggccacga gtcccgacgg ggcggccggc aggacgtcga cgtcgaggct
     3781 cgccgtggcg gcggcggcgt cggtcaccct gacgcggtcc ttggcggctc cgacggcatg
     3841 cccggcggca tagagccccg tccccgcgtc gacggagccg cccgagccgg agacggccag
     3901 ggaatacgcg tagggcggcg tcccgccgta ggcctggaag ctcgccgtcg cgccctcctc
     3961 cacggccgtc gaggaggagg agaggcccag ggccccgcct gagacgacgg tgaccgtcgc
     4021 gacggcgacg gcgggcggcg aggcggaatc gctggcgcgc agcgtcgccg tggtcgcgcc
     4081 gggtggcgcc gagtagaggc ccgtcgcgtt gacgcttccc aggccggcgg tcaccgcgaa
     4141 ggcgtagggg ggcgttccgc ccaggaccac gaactggaag gtcgaagcgg cggtgatggt
     4201 cgtcgacaag ggcgtgatcg tcagggcggc gggcgccagg acggagacga aggcggtcgc
     4261 caccgagcct tcgccgtcgg agatcttcac cacggcgtcg ccctcggcag aagcgaggta
     4321 gaggccggcc gggctcacgc ttccgacgcc ggagtacagg acgaaggaat aaggcggctc
     4381 tccgcccgcg ccctcgaact ggagggcggc gccgatctgc agcgccgcgg ccgccggcac
     4441 gatggcgagg gcgtgggact cgaagacgga gaccagggcg ctcgcggtcc gtcccccgct
     4501 gtccacgacc tccaccaggc actcgcccgc ggtcgccggc gcggtgaaga gtcccgtcgc
     4561 cggatcgatg ctccctccgc cggagcgaag gcggtagcgg tagcccggcg tcccgccgga
     4621 ggcctcgaag ctccggcttt cggcgaccat gaggtaggcg gaggaggggc tcaggctcag
     4681 gggctccagg aaggcgtcgt agaggacgaa atccccgcag gcccaggtca gggcggcgag
     4741 gacgacgagg acgcgcagca tcacgcggcg cagcatgggg acctcctaga ggcgccaggc
     4801 ggcgacaagg cccggcgcga ggccgagaag gggaaggttg cgctcgaaga cggccaggag
     4861 gccgacctcg agtcccaggg agaagccggg actcggcagg aattccaggc cgagtccggc
     4921 ggagcaggcg ggaaggatct tgccgagagt ctcgccggtc tcgggagcga ccgcgaggta
     4981 gaggggacct aggcccgcgc gaaggaaggc gctgagcctg ccttcggctg gccaacggcc
     5041 cctgaattcg gggcccaggg aggccaggtg gacctcgccg ccgacctggc cggccgagta
     5101 gaggagggcg tagccggcgc gcaggccggc ctcgagggag aaggagccga atcggaagga
     5161 gtatcccaag gagcatgagg cgtcgaagcc gaagcggaag tagtcggagg ccgccccgac
     5221 gacgaggacg ggggccgtcg acagaccgag gcccaggcgg gagagggcgg gaggaccgcc
     5281 cgactgcggg atgaccaggg cctccccgcc cgcggaaatc ggaacggggc cggtcatcaa
     5341 gaccgcctcc ccgcctgcgg gcgcccgggc caggaccggc gcctcggccg aggcggaggc
     5401 cggggcggcc gcgcccgctc ggggagccgc gtccccaggg agagacgcga ccgccggccg
     5461 aggcgcggga ggaggccccg gctcgaggcc ggagagcgcg acgagggcgg agacctcctc
     5521 gatcaggcgc tcgtccaggt ccagggtcaa gggcccggag agccgggcct cggccaggag
     5581 ggcttggccc tcggcggccg agcggagctc caggctgagc tcgtagccgc ccgcggcgag
     5641 gctcgaggag cagaggagga gggcctcggg cgggtcctcg cccgccccgg agccctgggc
     5701 aggagcgaag ccggcacgct ccagggccag gacggcgcaa cgctcgacaa tgcccgccaa
     5761 gcccctggcc tcggctccgc cgctcgactc gatgaggatc ctcgtctggg cgtgggacgc
     5821 gaggggcagg accatggcgg cgaggacgag gaggattcgg ccaggcttca tcgcctttcc
     5881 ttccttatcc gctgcgattc gtagatacag acaagtatag cgcggccggc cccgtcttca
     5941 aggaccaagg ccgcgcatcg tcgcttcggt ccccccgcac cggcccgcga gtggccgccc
     6001 gcgatgggcg gcgcgggccg cccccctgcc ccggggcgcg gctactcgag ctccagcgtc
     6061 atctcgtact ggcccacgtc ctcgcggatg cccgagcggg caaggagggt cctcgtgcct
     6121 gcgtcgtcgg actgcgcgtt gatgtaccga agagcgcccc cggtcttcgc cgcgagggcg
     6181 cggaggaggg ccgtgccgat tccccggcgc cgcgcctccc tggccacggc cagctgccag
     6241 atcgtgcccg agggcgaggc gaccgcgtag ccgaggagcc ggccggccgc ggcgcccgcc
     6301 gcgcccggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gggctcccag
     6421 tcgcgcatcg cggcggcctc ggcgcgggga aaggcggcga gctccccgac ggccacgccc
     6481 tcgggcgggg cctttccggg atcgacgaag gtcccttccg agcactcgaa gtcgcgggtc
     6541 ttgcggaagc cggcccgctc gtaggtcttg agggcgggcg cgttgtcgcg gatgacctcg
     6601 aggaggtaga gcccgacgcc ccgctcggcc aggaggccct tgagccgctc ggcgaggagg
     6661 ccggccaggc ccgagccccg ggcggagggc acgacccccg tccccatgtc gtaggccgcc
     6721 ttgcggccgc cccattcgcc cgagccgctc atcacgaagc ccacgaggcg gccgccctcg
     6781 taggcgccca gggagaggga ccagtcggcg ccgcgccgcg cgttcacggc gtcgaactcg
     6841 gcctcgctcc aggccacggg gacggaatag tcggaaaaag cctccatgaa ggcggcgtag
     6901 cgccgtgcgg gcgcttcgga gtccagggtc cggaactcaa acatggtttt cacctctcgg
     6961 cggccattca cggacggccg gaacgggttt actatgggtg ggaccgacga gcatttcaat
     7021 acccgccggg cgaagggaga ttcaatatga aggaagcggg agccgctagg cgcatcctcg
     7081 tcctcaacag cggcagctcc tcgctcaagt acgaggtgtt cctcatgccc aaccgcgaga
     7141 gcctcggcaa gggctccgtg gaaaggatcg gcgaggcgaa gggcatactc acccagacct
     7201 cgcccaaggg gcagatcagg ctcgacgagc ccatcaagga ccacggcgag gccatggcca
     7261 gggtcgcgaa ggccctggtc gaccccaagg cggggatcct cggctccatc ggcgagatcg
     7321 aggccatcgg ccaccgcgtc gtccacggcg gcgagcgctt cgcctcctcc gtcgtcatcg
     7381 acggaccggt cctcgaggcc atcgaggcga acgtcgagct ggcccccctc cacaacccgg
     7441 ccaacctcac cggcatccgc gaggccgagc gcctcctccc cgggataccc caggtcgccg
     7501 tcttcgacac ggccttccac cagaccatgc cgccctcggc ctacctctac ggcctgcccc
     7561 gcgagctcta cgacaagtac aagatccgcc gctacggctt ccacggcacg agccaccgct
     7621 acgtcgcggg ccgcgccctc gagctcctca agcgccaccc cgacaacacg aacctcatca
     7681 cctgccacct gggcaacggc gcctccatca ccgcgatcga gcagggccgc tccatcgaca
     7741 cctccatggg cttcaccccc ctggagggcc tcatgatggg cacccgctcg ggcgacatcg
     7801 acccggccat cctcggcttc ctggaggagc ggggctacac ggcgaaggac ctctcgaacc
     7861 tcatcaacaa gaagtccggc ctcctcggcc tctcgggcat ctccaacgac ctccgcgacc
     7921 tggaggccgc cgtcgacgcg aaggacaaga acgcgacgga agccctggag gtctacgcgc
     7981 accgcgtgcg gaagtacatc ggcgcctact ccgcggagct cttccgcgtg gacgcgatcg
     8041 tcttcacggg cggcgtgggc cagcacggct ggcgcatgcg cgagcggatc tgccaccgcc
     8101 tggagaacat cggcatcatc atggactacg agaggaaccg gacggacggc agcgcggagg
     8161 gcgtcgtctc ggcgccctac tcccccaccg ccatcctggt cgtccccacg aacgaggagc
     8221 tgcagatcgc ctgcgacgcc ttcgagctcc tcttcgcgcc cgaggagctc atgcgccgca
     8281 agtcggacgt gcaggtgtag ccgagccgtc gattgaccct gggcgccgac gcgtggaaga
     8341 tgggcaggcg gagaacatat gccccgagag aaagccttcc tggacaccag ggtcatcatc
     8401 gacaagacga tagagctcat cgaggccgag ggctacgagc gcttctccac gaggcggctc
     8461 gcggcggccc tcggcagctc ggtgatgacc ctctacaact actacgagaa ccgcgaggcc
     8521 atcctccgcg aggctctgct cggctgcttc gaaaaccttc ttgcggggat cgaggcgagg
     8581 ctcgagccct acctctcggg cgagcggggc tcgccgcttc gcgcctacgt cgccctgggc
     8641 gagcttctcc gcgccttctc cctggagcgg ccgaggatct acgccttcct cttcgaggcc
     8701 aacctcgtgc cgctgaggac cgatccgcgc atagccgccg tctacggcta cacctacggc
     8761 aggatcgagg ccctactccg ggatccggcc cggagcgggg agctccacga ccggatctac
     8821 ctcttccagg tcctcatgaa cagcctcgtg aagaacgccg ccagggggcg gggcggcatc
     8881 gacccggccc gcttcgaggc cctcctggcc gaggcctact cggccctcct cgcgccctac
     8941 gaggggctcg tggctccgta gggcttccgc ccggccaggg aggccccgca gacggggcag
     9001 gtctccgcgt accaggagtc gggcgcgccg cattccggac acctccagag ctcctcctgc
     9061 ccgcggagcc aggcctcgac gccccgctcc gcgatcgcgg cgagattgcc cgcgttgagc
     9121 ttgagatggg gcagctccgc gaggaggcgc tccgcgtccg gcctcgagta ggccgcgcag
     9181 gggaactcgg ggcaggcggc gcaggactcg aggccccgct ccctcgcgca ggcgcggatg
     9241 gggcagcgcg cgcagttccc gaagacctcg tcgctcttgc agcccttgca tcgccaatcc
     9301 ccggcgggag ggaccctctc cttgaggtag gcggagaagg ctcccggctc cccgcggcgg
     9361 caggccgcga ggatgtcgca ggcgccgcag tagatcccgc agtacgagtc gcagcggctt
     9421 ccgtccatgg tccctccgtc aggcatgagc ttctccttca ggcgacccag tactcgctgc
     9481 cgtcgggctt ccgggccagg aaacgatact cgatgaggta gcggcggatc gtcacgtggt
     9541 cgtcccagag gggcgagagg atggcgttca cctcgggctc cgagtagcgc cttcccgcct
     9601 cgaagaggcc ggccgcgcgg cggaggagga caagcttctc cttctgccgc ttgggccagg
     9661 aggcgaaggc gaagcctcct cccgggaggt cgcggagatg cctcgcctcg atggcggccg
     9721 cctccgcctc ggtgacggcc gcgcgctcgt ccttggccgg catgcccgcc ggatagtcga
     9781 ggaagcggcc ggcgcccgag cccccctcgt ccaggaggcg catcagggcc aggaagaccc
     9841 gcgcctccgc ctcgcgcttc cggaggttga agcggtggtt gcggacggtg gagggggatt
     9901 tgccccccag gagggcggcg atctcctcgt cgctccttcc ctcgtggagg aggcccaaga
     9961 ccctgccctg cacctctggg aggccggcgg cctcgtcgcc caggccgcgg aggagggcga
    10021 aggccccgcc gtgggcccgc tggacgtggg cgcgcgccgc gcgctcggga aggaggaagg
    10081 cctcgccttc ctggtaggcc cggccgtcct cgaagagggc gccgcaggcc aggcaacgga
    10141 agcccgggcc cgcctcgtcg cgggcaaagc cccgcgccag gtcgtcgacg gaggctccgg
    10201 atagattgag ttcgctcatg tcgtttattg tatagacagt tatgatctct gtctatgcct
    10261 gtagatcatg aagcggtgaa cccctcgcag agtcgagggc ttgacgtaac ataacattcg
    10321 ttatattaca tccgtgatac aggagcggcc gatgcccaga aacctgacct tcaacgccga
    10381 atccgtcctc gacgcggccc tcgaccttgc gcgcaaggag ggcatcgagg ccctctccgc
    10441 ccgcgccgtc gccgcgaggc tcggcgcctc gacggcgccc gtctattcgg cattctcctc
    10501 catggagagc ctcagggagg ccctcttgga cgaggccctg gagctcctgg acgcgagcgt
    10561 cgagcaccgc tacacggaag gacgtttcct gaaccaaggc gtcggcttcg ccgtcttcgc
    10621 ccgcgacgag ccccggctct tcttcgccct cttcgacgcc ggcgagggcg accttctgga
    10681 gcgctaccgg gcgagcctcg tggaccgcct cttcgacgat cccgccctgg ggcgcctaga
    10741 aaggccggcc atcgagcgca tcgccggacg gatgtggacc tacacggtcg gcctcgcctt
    10801 ctaccttgcc cacgggcgca gggccgacgc ctccacggga gccctgatcg aggagctcag
    10861 gagcgcgggc tccgtcgcga tctacggcga gctcaagggc gtggccgact acggcagcgc
    10921 ggaggcgagg gaggcctggg ccgagctcgt cgcccgcaag gggctcgacc tcgagctcaa
    10981 gcccctcgcg atgcgaagcg aagtgaactg atcccggccg atagcggccg gaaaaggagt
    11041 caggcatgtc tgcaaggaat ccccgcgcct acggcgccct tctcggactc tcgggcctct
    11101 tcgccctggg agccgtcctc accctcctgc ccaatccggg cgcgagctgg gagaacgtcc
    11161 tcggctaccg gagcctctgc gccttcgcgc ccatcgcgac ggcgctctgc gccctcctcg
    11221 cgggcgcgac ctgcgtactc cgcgcccgcc tcttcgggcc gcgcgcgggc gacaggcgga
    11281 gctgggccgc gccggtcgcg gtcggcctcg tcctcctcgc ggtgatcgcc ttctcggtcc
    11341 cgccctacgc gagggccaag gccgaggcgg tctcgggggc gagcgccctc gccgagtagc
    11401 gcgggccgga ccggcgcgac ggcggcccga tgacgccgcg cgcggagccg cctggcgcgg
    11461 ccccgcctgt cgcggcggcc cgtcaccgcg agggctacag tcccagcgcc ttcgtgagga
    11521 ggccgaagag gtactcgccg agctggacga ggagaccgaa gacgaggccg gagacgatgg
    11581 aggccaggaa ctccgtccgg cgctcggcct cctcctcgat cctgcccgcg cgggcgacga
    11641 tctccacgag gacgatggtc gccgcgagga agagggccag ggtggcgctg aagtagagga
    11701 cgcccaggga ctggacgtag cggtagagct tgtccgtggc gaggaagatc gccgcggaga
    11761 cgaggccccg ccagaaggag acgaagtact ggcgcacccg cacgcccttc cgcatgaggg
    11821 gcagggcgat cgccgcggcg atgaggacga gggtccctat ggagaccccg gccacggcgt
    11881 tcgcggagaa ggtcgtgtcg atggcggcga ggctctcgtc ggccttctcg atgacgatgt
    11941 cgaaggggtg gaagtaggcc tggacgaggg ccaggccgcc gcccgcgacg aggaggagga
    12001 caagggccca gcccagtccc ctgacatagg agaggagccg ggccgtgctc gccctgacgg
    12061 cgttgtggcg catgccggga tggggtctgt tcatgcctcg agcctagccc aggccgcgcc
    12121 cgcccttcaa gggccgcgcc ttccatcaag aagccgctcc aggcttcagg gactcgaccc
    12181 cgcgcggtat actcgaggca tgagggacag cgagaaagcg gcgagcccgg gcctaggcgg
    12241 actcgccgag gcgctgcggg aggccctctc ccgccccctc ccgggccgct ccgcccagga
    12301 cctcatgctg cccgcctatc gcctgggcct gcccgaggag cccgaggggc gggatggctg
    12361 gcgccaggcg gcggtcctcg tcctcctcta ccccggaggc ggtccgcccg ccggggccgg
    12421 gtcgagtcca ggggcgggcc ggctcgccgc ggccgacccc ctcgccgggg ccggccccct
    12481 cctcttcccc ctcatcgaga ggagcaccga agtcgggcgc caccgcggcg aggtcggcct
    12541 ccccggggga gcccttgagg cggacgagac cggcgaggag gccgccttcc gcgaggcgcg
    12601 ggaggagctc ggcctcgagg ccgaggccgc ccggcccgcc ctgctcggcg cgctctcgcc
    12661 cctccgcgtc ccgccgagcg gctacgaggt gcggccctat gtcgccctcc ttccccgaag
    12721 gcccgccttc aggcccgagc cggctgaggt ggcggagctc ttcgaggcgc cgcttagccg
    12781 cctcctcgag cccgaggccc ggggcgagga ggagcggctc ttcggaggca ggcccttcct
    12841 cgtgcccttc ttcggcctgg gaggacgcaa ggtctgggga gcgacggcga tgatcctcgc
    12901 cgagctctcg gccgtcctcg agtcccttcg gtaggcgcgg gcctggcgga acgaggccgg
    12961 ccgcgcgagg ccgggccggc cgccgcgacg cccgtcgcgg cgcgggattc cgcgtcgctt
    13021 ttccggtggc gaagccgcgg cctccgccgc tagagtaggg aaggaggcca cagatgagcg
    13081 agctgccccc cttcgaggaa atggacaagg cgttccggag ccgggacgag gcctacgacg
    13141 gcctcttcta cgccgccgtc tctacgaccg gcatcttctg ccgtccctcc tgtccggcca
    13201 ggaagcccct gcccgggaac gtccgctact acgcgaccgc cgcggaggcc ctcttcgccg
    13261 gctaccggcc ctgcaagcgc tgctcgcccc tctcgggctc gggcgatccg ccctggctcg
    13321 gcgcgctagt ggagcgggtc gaggcggatc cccgcgcgcg gatccgcgac gcggagctcc
    13381 gcgccgaggg cctggacccc gcgacggtga ggcggcgctt ccaggcccgc ttcggcctca
    13441 gcttccaggc ctaccagcgg gcgcggcgcc tggccgccgc cttcgaggcg atcaaggagg
    13501 gcggctccat cgacgacgcc gtcttcgact cgggctacga ctcgcactcg ggcttccgcg
    13561 aggccttcgc gcggctcttc ggcgccccgc ccggggcggc gaaggcgggc ggcgccgcga
    13621 aggcgggcgg cggcgcgcgg gcgggcgcct ccgggacagc aggcgcggcg gccatcccgg
    13681 cggacaccga cgcggccgcg ggcaagctcg ccgacttcat ccgcctttcc tggatcgaca
    13741 cgccgctcgg ccccatggtg gccgccgcga ccgaccgcgg cgtctgcctc ctcgaattct
    13801 ccgaccgccg catgctcgag gcccaggcga agatcctctc agcgaggatc ggcctccccg
    13861 ccctgccggg cgcccacccc cacctggagc gcctggccgc ggagctcgcc gagtacttcg
    13921 ccgggaagcg ccgctccttc gaagtgcccg tcttcgagcc gggcacgccc ttccaggagc
    13981 gggtctgggc cgccctgcgg gagatcccct ggggccagac ccgctcctac gcggagctgg
    14041 cccgcgcaat aggcgatccc gccgcgaccc gggccgtcgc gcgggcgaac gggatgaacc
    14101 gtgtcgccat cctggtgccc tgccaccgcg tcatcggcgc cgacgggggc ctggggggct
    14161 acggcggcgg cgtctggcgc aagaagcgcc tcctcgaggt ggagggagcg gcctccccgg
    14221 ggaggtgagc ccggcctgga cttggggcgc aggtccgcgg ccggatccgg ggggccgcgc
    14281 gggccttgga ctcgcgacgc aggtccgcag ccgggccagc ggcgccggcc gagccgggcc
    14341 cggcctggac tcgcggcggc cgggccgcgg gagcggcggc gcgcaggggc ttgtcagcta
    14401 ggcggcctcg ggccgggggc cttgccacgg ccgggcccgg ggcgcgagga tggagccatg
    14461 gacaagagca agaccatagc cctggtagcg cacgacaaca tgaagcgcgc cctcatcgag
    14521 tgggtcgagt acaactggca cacgctctcc aagcatcgcc tggtctgcac ggggacgacg
    14581 ggccgcctcg tggaggaggc gatcgccgcg aagaacggcg gcgtcctccc ccacggcttc
    14641 agcctgacgc gcctcaagtc gggccccctg ggcggggacc agcagctggg cgccctcatc
    14701 gcggagggca agatcgacgc cgtggtcttc ttctgggatc ccatgtcgcc ccagccccac
    14761 gacgtggacg tgaaggccct gctcaggatc gccgtcctct acgacgtgcc gatggcctgc
    14821 aaccgctcca gcgccgactt cctcatctcg agccccctca tggggcgcga gtaccacccg
    14881 acgcccctcg acctctcggg ctacgtcaac cggaagatcg gctagagcgg gcggaggagc
    14941 tcctccctcg tcattccccg cctccttccg cgccgcccag ctggccgcag gccgccatga
    15001 ggccccggcc gcggggcgcg cggagcttca cctggacgcc cagggcgcgg agacgcgcct
    15061 cgaaggcctc gacctccgcc tcggtcgggg cggggccgag gggctcggag ccgggattgt
    15121 aggggatgag gttgacgagg gcccggccgg gctgggccgc cttggccagg aaggcggcga
    15181 cgccctccgg ggcgcccggc ccgtcgttca ggccggggat gaggcagtag ttgaccgcga
    15241 gggcgaagtt cctccgctgg ggataggccg ccagggcctc cgccaggcgg tccaggccgg
    15301 cgccccggtt cgcgggcatg agggccgagc ggatcccgtc gtcgggggcg ttgaggctca
    15361 cggagaggtt gaggcgcttc aggcccaggg ccttgagccg cgcgatgccc tcgggcaggc
    15421 cgaccgtgca gacggtgacg cgctcctggg agaaggccgc gccggcgggg tcgaggagga
    15481 cggcgagggc cgcggccagc tcctcgaaat tgtccagggg ctcgcccatg cccatgaaca
    15541 ccaggttgcg gatgttccag ccgaggagga cgcgggccgt gacgagctcc gcgacgatct
    15601 ccgccgcgcg gaggtcccgc gaaaggcctc gcttccccgt ctcgcagaag gcgcagccca
    15661 tgcggcagcc gacttgggtc gagaggcaga gggtcgccgc gccgcccgag gcgacgggta
    15721 tgcggacgca ctcgacggtc ccgccgtccc cgagctcgag gagggccttc gcggtgacgc
    15781 ccagcggccc ttcctcctcg acgacgcgaa ggggctttaa gaggccgacg cggaagcggg
    15841 aggccaggga ggcggccgcc tcggccgaaa tgccggtcga ctcgggcccg aagcgccccg
    15901 tcgcgaaggc ctccttgtag agggcgccca gggcgcgcga gcggcccgcg cccaggcccg
    15961 cggcggcttc gaagcccgcg cgggtgaggc cgaggatgtc gagcggcgct tgggcgggga
    16021 gcggcatggg cgggaacctc cgccgccatt ctccaccgca gcggcgtcct tcggctagcg
    16081 ggccggccga gccgaccgcg gaaaaaccgc cgcggcgcga ggcgcgcggc ggccgatccg
    16141 gcgctatagt atggggaggg ccggcgccgc gcgggcgcgc gtccctggag ggggctggca
    16201 ggatggcggc gaaaaagaag gcggcgggcg acaggctctt catggacaac ctggcccagg
    16261 ccgtccgcga ggaatacgtg aaggtcgcgg tcgaggaggc gagggcccgg cggcccctgc
    16321 ccgccctggc cgcgcccgag gcgcccaaga cgacgggggc ggcggccaag gccgccggca
    16381 ccaaggccgc cccggccgcc tcggcgagga agccggcggc ctccgagaaa cccaaggccg
    16441 ccgaagcgaa gccggctatc gccaagtcgg ccgccaaacc tgtcgccaag gccgccggca
    16501 agaccgcctc ggccaagaca accggcaaag ccaactcggc caaggcctcg aaggccgaga
    16561 aggccgaagc cgaggagaag gccctcctcc tcaaggatct ccacgccctc ctgccgcggc
    16621 tggacgcgga gggcctcgcc ttcctcctcg agcaggccga ggtacacctc tacaacatgg
    16681 aggcggaggc cctggaggcc tcgcgggccg agcgccagcg ccgcgcggcc gaacggcgcg
    16741 gcgtcgcgcc gggggcggcc ggcggcctgg ccctggtccg ctcggagtcc ggctcgagct
    16801 actacgtgga ggcgggcgac gcctccgtca tgttcaccaa ggaggagatg ctctccctgg
    16861 tgcgcctggc ccacggccat cccgacgacc tcgaggccgc gagggccctg ggcgcctggc
    16921 tcgagcgcga gcgccgcgac gccctggacg agctcaggct ccccggcgcc caagggcccg
    16981 cgctcaagga gctcgcccgg ctcctcaggt cgagtttcaa gaggccggga gcctgaaggc
    17041 cggctcgcgc gcgggtcagc gcgcgggtca gcgtggcgct acagtggcgc ggcggcgcat
    17101 cgcggctcgg tgcagtgcag cgggtcgcgg cgcggagcct gacggaaggc ggccagactc
    17161 gatccgcccg ggctactccg aaggcgccgt cctgaagagc ctgaagccgt tgtcgcccgg
    17221 gtagccggcg ttgtcgaggc tccggaaggc gacgcggacc gactcgcggc cttccctcca
    17281 gcttcccgcg cgccgggaat gctcgggccc gcgcggcgtc gcggggcgcc cgggaggcgg
    17341 cccctcagga tcaatctccg gggaggccgc gtagtagtcc cggccgtaga aatcccagca
    17401 ccactcgtag aggttccccg acatgtcgta gaggccgagc tcgttgggcc caaggaggcc
    17461 gacttcgcgc atcgcgcctt gcgagttctc gccgaaccat gccaccgcca gggggtcgtc
    17521 gctccccgcc cagcggaagc ccttggagag gcggccgccg cgcgcggccc actcccactc
    17581 ggcctccgtc ggcaggcggt agcccgaggc cccgaagtcg caggccgtct tgagcccctc
    17641 gcccgaatag cagggcgcaa ggccgtgcaa gcggctcagc cagtcgcagt aggcgacggc
    17701 gtcgtaccag tcgacgccga tcgcggggag gcgggacccg tcgaagccga ggcgcgactt
    17761 gggcctggcg cggcccgtct cgtcgcagaa gcggtcgtac tcctccgcgg tgaccttgcg
    17821 gacggcgacg aggaggtcac gcgcgatgcg gacgcggtgc gccggccgct cgtccgcggc
    17881 cccgtcctcg ctccccatca cgaaggcgcc cgcctccgcg cggaccatct ccgggagggg
    17941 cggcggcgag gcgggggctc cgggcgcggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn ngcgcaggcg cggaggcttc gtttgtccat gaggcgagta tcgaccctcg
    18121 ccgcgcgccg cgcaacggcg ccgcatgggg acttatcggc tgtactcgac atttcccgcg
    18181 catggcggcg cgcacggggc gcctgcgggg gcgcgctcgg gaggcgcggt tccgcgcgcg
    18241 cggaagcgcc ggaaggcgcg ccggagctca gccgctgccc ggggaggcgg ctcggcggat
    18301 caaccgcggc ccggggaggc tgctcggcgg atcagccgcg gcccgccgac gcggcgtcgc
    18361 cggacaggag gcgcacgagc tcgacgcggt tccgggcgcc ggtcttctcg aacatgttgt
    18421 gtacgtggtt cctcaccgtg ttggcggaga ggcccagggc ggccgcgatc tccttgttcg
    18481 ccgctcccgt cgaaaggagg gaggcgagct cgaactcgcg cccggtgagg cccagggcca
    18541 gggcgcgctc gcggtcggtc ggccccgagg cgcccgaggc gagggcgccg gaggcctcgt
    18601 agcgcgcggc ccaggccagg gagaggaggt accaggcgag atacgtggcc ggccttaata
    18661 gggcgccgta gggccaggcc ccggcggcca gctgggcgga ggagaggccc gagtcgatcg
    18721 ttccggcggc caggcagaag gcgttgtagg cgaggatcgc ggggatgccg atccgtccca
    18781 ggcggtcctc cacgcccctg taggcgcgga tcaggcggta ctggctgtag agcatgaggc
    18841 ccaggaagga gagaaggccg aggctgggcg ggacgaagcg gaggaagggc gcgccgccct
    18901 ccaccagggc gaggggcgag gaggcgaggg tcgccgccgc gaggccgatg gccgccctcc
    18961 tgggcccgcc ccggaagggc aggtcggcga actcgaggaa gaagcgcggc gcggcgtagg
    19021 ccaggatccc cgaggcgagg acctcggaga aatagtacgc gcgggagagg gcgtcggggt
    19081 cgggggccag gccggccgcg gaggcgtaga gcccggcgag gccggcaagg agctggaaga
    19141 ggccgcaggc gagatagacg aggtaggagc ggacgagggg cgagcggccc gctcggtaca
    19201 ggccgaggaa gaggccgagg acggcgagtc ccgtcccgaa ggcggcgagc gtggcggcga
    19261 gggcgaggtg ctgcacgggg gagatcctac gacgcgggcc gcggccgcgc aagcgccgga
    19321 tttccgggct tttggtacga aactaccagg cccttgggcg ccgtccggta gctaattccc
    19381 atgggcccgc cggagccgcc cgggctagat tcgccgcgtc gggcgcaagc gggcccggaa
    19441 ggagctgaca tggaaggcaa gctgggcgcg gccgtggagg cgcgcggcct ggagaagagc
    19501 ttcgggtcga gaagggcgct gcgcggcctc gacctgtcga tcccgcgggg cggggtcttc
    19561 ggcatcctcg ggcccaacgg cgcgggaaag accacgacgg tgcggatcct cgctgccctg
    19621 gcgcggccgg actcgggcag cgtcgaggtc ctgggacgcg acgcgcggaa ggaggcccgg
    19681 gcggtccgcg ccctcgtcgg cctctcgccg caggagaacg cggtcgcggg gaggctctcc
    19741 gcgatggaga acctcctcct ggtcgcgggc tcctacggcc tccgcggccg ggacgcgaag
    19801 gcccgcgcgg aggcctgcct ccgcgccctc ggcctggagg accgggcccg cgaccgggcc
    19861 tccgcgctct cgggcggtct cacgcgtcgc ctctccatcg cgatggccct ggtcgccgat
    19921 ccgcccctcc tcttcctgga cgagccgagc ctgggcctgg ccccggaggc ccgcgccgag
    19981 ctctggcgcg tcatcgcgtc gacgaagggc gagaagaccg tgatcctcac gacgcactac
    20041 ctggaggagg ccgaggccct ggccgaccgc gtcgccgtcg tcgtggacgg gaaggttgtc
    20101 ctggaggaca gcccggcggg catgcggcgc cgcgcggggg gaggcggacg cgtccgcctc
    20161 gagctctcgg gcgaggagga ggctgcgcgg ctcgccgcgg ccctcgcagg cgccttcccc
    20221 ggcctcaggg cctcgggcgc gcggctcgag ctcgagggcg gcgaggccgc cggcctggcc
    20281 ctcctggacc gggcgcgggc ggcgggggcc gtccccaggg gcttctccgt cggagaggcg
    20341 agcatcggcg aggcctacct cgccgcgatc ggaaagggag gagaggcatg aggtggctgt
    20401 cgctcgcgag gcggaacctc gcggagatac tgagggatcc cttgagcgcg ggactcggcg
    20461 cggcgatgcc ctgcgccctc ctcacccttt tcgcggcgct cggaaggagc gtgcccgcgc
    20521 cccttttccg catcgggccc atgacgccgg cctacgcggt cttcagcttc gcgatgctcg
    20581 tcatgttctc ggccatgctc ctttcgcggg accgctcggg cggcctcttc ggccgcctcc
    20641 ttgcgactcc cctcgagccg cgggacttcg ccctggccta cgccctgccc ttcctgccgg
    20701 tcgcggcgct gcaggccgcc gcctgctacg cggtcggcgc cgccttcggc gcgcccctgg
    20761 gcgcgggggc cgcggcctcc gcggcggccc tcctccccgc ggccctcggc ttcgtcggcc
    20821 tgggcctctg cctgggcgcg ctctgcacgg agaaccagat cgcgggcctg ggctcggccc
    20881 tggtcaccgc ggtcggcgtc tttggcggcc tctacttcga tcccgcggcg gcgggcggct
    20941 tcttcgccgc gatcgggagg atcctgccct tcgcctccgc cgtggaggcg gcgcggctcc
    21001 tcttcgcggg ctcgtgcctg gcggccgcgg cggcgcccct tttcaagacc tgggctttcg
    21061 cggccctggc cctcggggcc ggcgtcctcg ccatcagggc cagggccagg cgctgagggt
    21121 tcccgcccgc cgccgcgggc ctggccgccc gccgcgcggc gttgggcgct atccgaagcg
    21181 cccggcgcgc cgcctcgggg acggcgcctc ggggacggcg cgctacagct ccatgcccag
    21241 gaggaggtac tcctcggaga taccgtggcg cgcgtagaag cggcgcgcgc cctcgttctc
    21301 ccgctcgacg ccgacctcga tctcgtccat gccgcgggcc ctggcccgcg cgacggcccg
    21361 ggcgaggagg gccgcgccga ccccgccgga gcggcgccgc gcgtcgacga cgagctcgtt
    21421 gacctgcgcc gtcccgacgc ggtggtaggc gctctcgtag aagaggaggg agacgaagcc
    21481 gaggacctcg ccctcctcct cgtagacgta ggtctcgtag atcccgggga gggccgccat
    21541 ccttccgaac atcgcgcgga gccgaccctc gtcgggccgg aagtccccgc ccagggcctc
    21601 gtcgagctcg cggaccaggg ccgcgatcct gggcaggtct tcctcgcgcc agggccggat
    21661 tgcgccggga ctggtcacat cgggcctggt cacgccggcg cccccgccgc gccgcctggg
    21721 accggcgcgc gcttcatcat gaggatctcc tctactccgg ggaggaagag gcccggcacc
    21781 gcccccacgc gctcgtagcc gcgcttcccg tagagcgcga tcgcctcggc gttgaagtcg
    21841 gcgaccatga ggaaggccat gggcgagcgc tcctcgacga aggcggcgcg ctcgaacagg
    21901 tccatgagct tcccgccgat cccccggccg cgcaggtccg cgcgcacggc gacgacgtgg
    21961 aggaagggga accagtggaa gatgcccctc ggctggatcc aggcgaaacc gaggcaggcc
    22021 ccgtccccgc ccagggcgac gctgacctct tccttctcga agccctcgcg gaggagctcc
    22081 ccgccgatgt agcggccccg cgcgtcgcag aaatagcggc gcccgagctc cgagccctcg
    22141 ag
//
LOCUS       DAOOSJ010000012        21608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1670_length_21608_cov_7.404816, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000012 DAOOSJ010000000
VERSION     DAOOSJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 21608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21608
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_21608_cov_7.404816"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1274)
                     /gene="dprA"
                     /locus_tag="PLG14_01405"
     CDS             complement(198..1274)
                     /gene="dprA"
                     /locus_tag="PLG14_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOX47812.1"
                     /translation="MPSDAGRRIVLLALQRLELMGAAGKLRLLAALDSGAELASLSRA
                     DLEAIAGRRLRAGRWDPEALLSAAEADARLLERMGAFYLQRDEAGFPAALREIPGAPF
                     GLYFRGERLPEGLPAVAVVGTRRPTGRGLLAARSLSRELSLAGLPVVSGLALGVDAEA
                     HRGALEGRRRSPLAGPTCAVLPCGVERVYPAAHRGLAAAILASGGLLVSEFAPGTEIR
                     KSSFPERNRVISGLCRGILVVEAPSGSGALITADFALEQGRDVFVAASRLAGPRSAGL
                     DGLASDGALAVASARGVLAEWGIPAAGLDGLADRDARGSFPGYYGAGEGSFLAEALRA
                     ELGLDLAALAARPEEPGARQYGQA"
     gene            complement(1264..2058)
                     /locus_tag="PLG14_01410"
     CDS             complement(1264..2058)
                     /locus_tag="PLG14_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX47813.1"
                     /translation="MDPRQAVGTERGRPRGAAGRRRAGGAVRVLLALAALGLLSSCGP
                     DGDLKRLLDLEDRSAVGAPPQSVEELKEGIAKYSKEAERVVLADEKVGIYYKILASRL
                     LEKKLYGEAYEALAKAVEYFPSNSNLYYNAGIAAGYIAKSKLALGPDGEPERRKWLLT
                     SESSYRAAIDINPRYGAALYGLAVLYEFELGRPADSVPLLLRLLEIETRNADAMLLLG
                     RVYYRLGRLEEAVNVYETAARVTRVEAKKKAAEENRERILGEMNAE"
     gene            complement(2118..3158)
                     /locus_tag="PLG14_01415"
     CDS             complement(2118..3158)
                     /locus_tag="PLG14_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX47814.1"
                     /translation="MDDLLRRYRAYLLAARRRSPLTAEVYLREASHLESWLAERGLDP
                     ATATSSDVLGFLVWRQSGAAALRERESEEQGSPADRGATQGAPRRESEEQGSPGDRGA
                     TQGAPRRESEEQSSSLSRKTMARVVSSVHSFFKFLRLEGVREDDPSELIETPKQERQL
                     PAVLALEEIDRFLAAIDSGSPRGLRDRALFELIYSCGLRISEASALTFDGLFLDEKVI
                     RVLGKRKKERLVPFGDEAAAWLRRYLEEGRPALLKGGRSDRVFLNHAGKGLSRKGIWK
                     RFDLVRQEAGVDAKVHTFRHSFATHLLAGGADLRTVQELLGHSDISTTQIYTHIDSEA
                     LGDYHREYFPRK"
     gene            complement(3161..4348)
                     /gene="ftsZ"
                     /locus_tag="PLG14_01420"
     CDS             complement(3161..4348)
                     /gene="ftsZ"
                     /locus_tag="PLG14_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOX47815.1"
                     /translation="MNIELVEDRGADASPTVIKVIGAGGGGSNAVNRMIVAGLRNVQF
                     IVANTDLQALNLSRAEVKLGLGAKVTHGLGAGGKPEIGEKAALEDRDAIAQALKGADM
                     VFVTAGMGGGTGTGSAPVIAQVARDLGALTVGVVTRPFDFEGRVKARLAEEGIEKLRQ
                     AVDTLIVIPNQHLLKVVERRTPIKEAFQLADDVLRQGVQGISDLITIPGDINIDFADV
                     RTVMEGMGDAIMGIGAASGDNRAVESAGKAVNNPLLEDARIEGARNILVNVTGGDDLA
                     LTEYEEIVNLITESADPEALVIPGIVTDPALGDEVRVTVIATGFNRSLKTQNTAASAS
                     VREASRRGLEDYISSDEWKRITENPSQRAKQYLLSRNEGEDELDIPTVLRDRRLAGEK
                     SEA"
     gene            complement(4434..5672)
                     /gene="ftsA"
                     /locus_tag="PLG14_01425"
     CDS             complement(4434..5672)
                     /gene="ftsA"
                     /locus_tag="PLG14_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOX47816.1"
                     /translation="MADNLIVGLDVGTAQTRAVIGEYNENGVLEITGVGTAPSTGLRR
                     GVVVNIEATLASVAAAIEAAELMSGREAKDCFVGVAGANIEGINSRGVVAVTGKGREI
                     TQSDVDRVIEAARAVVIPMDREVLHVIPQTFIVDDQKGIRNPLDMIGVRLEAEVHIIT
                     GSVTTAQNLVKCVNRAGFKVERLILQSLAAARATLTSDEKELGCLLVDLGAGTTDLLV
                     YSDGAPYFTSVIPVGGAQVTNDLSIMLSVPMDAAERLKREAASAWLEGVDPEESVVVP
                     GVGGRPPVEISRRKLCTIAQPRMEEILRMARERVEKMGHWRHIKGGAVLTGGGALMQG
                     AAELAQEIFEMPVRVGNPITVGGLVEEYRSPVYATGVGLVLLGAESIAPERRAEAART
                     EKAKGPSLGKIFDWLKNGFF"
     gene            complement(5665..6603)
                     /locus_tag="PLG14_01430"
     CDS             complement(5665..6603)
                     /locus_tag="PLG14_01430"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOX47817.1"
                     /translation="MSEFALGRVSFEAAGARGNDAGRRRPRPEIRGAEGLGTAPARGR
                     GAGRRASVERALGLLLGAAALALAAAAAFVLVPPALRVSRYEVVGAATMSDREVLDAA
                     LVHGSEYFFALDPERVRAALLASPRIAGARVRRLFPNGLRIEVAERQAVAAVLAERNG
                     RLETVLVDGEGVAFAFASELAGASGLPPAGLADLPLVSGLRFEGFSLGTRLPPSLVPL
                     LASLGELEARAPSLLAAFSEIRIAKPKYGEPELVLYPLHHRVPVRTGAVLNEATLRSI
                     ILVLDVLGSRGLADGVEEIDFRRGTVVYRSKGGQSG"
     gene            complement(6596..7729)
                     /locus_tag="PLG14_01435"
     CDS             complement(6596..7729)
                     /locus_tag="PLG14_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOX47818.1"
                     /translation="MPSGFAVEPMARERTWDSALLASLFLLAGTGFAALWSASSGYAL
                     SIGKAPYHFALRQLLFLAPSVGLFWAGALAPLDLLRKKAGLVTLLGLGSLLLPFIPGL
                     GENRNGASRWIDLGFTTIQPSEFWKPVSVFYLAHVLAKRQEAKALGVAPLMPPFLLAG
                     SGCLLVFLQNDFSTAVIAAAAAVAVFWVAGAPLAFFAGLAAVGGPLAALSVLTSDFRL
                     RRILAFLFPAYEPHGQGYQVLGSLRAIRSGGFFGKGLGLGTLKLGSLPYVQSDFIFAA
                     LAEETGFLGVLAFFGLWGFFAFRAFRSSFREEDGFRSYLGFGLASLLLLEVLVNVAVA
                     AGAMPATGIALPFFSAGGSSLLATAATCGVLANLSRSEGGRHV"
     gene            complement(7741..8823)
                     /gene="mraY"
                     /locus_tag="PLG14_01440"
     CDS             complement(7741..8823)
                     /gene="mraY"
                     /locus_tag="PLG14_01440"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOX47819.1"
                     /translation="MLLELLYPLVKYFSPLNVFKYLTFRSAYAAVTALLIAFVFGPRL
                     IEKLRVLKFGQAVRSDGPESHLAKSGTPTMGGILIILAVASAVLLWSDLRNLYTWVAL
                     GSLLGFGLIGFLDDWRKIKYRNSDGLPAAAKFWSQVVIASAAALVLYFDGNPQTTLLY
                     VPFFKYPVLDLGALWIPFAVVYMVGWSNAVNLADGLDGLATGLTIIALIAFAILTYVT
                     GRADWSAYLSIPFLKGAGELTVFNLALIGACVGFLWFNGHPAEVFMGDVGSLALGGVL
                     GVLSLIVKKEILLLLIGGVFVMEVASVTLQVGWFKLSKGKRLFKMAPIHHHFELKGWK
                     ETKVVTRFWILGGIFAIIALSTLKIQ"
     gene            complement(8837..10348)
                     /gene="murF"
                     /locus_tag="PLG14_01445"
     CDS             complement(8837..10348)
                     /gene="murF"
                     /locus_tag="PLG14_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOX47820.1"
                     /translation="MAERLSFSAGEALAILRGAAAEYAPVALSRAGLAPAPGASAGLS
                     GLDASGIAAAFAASSLEGPAAARFFEVAVDSRKAGPGSLFVALPGEKADGHDYIAQAL
                     EAGASCVLAEERGWKARASALAPLAARKGAAVLLVPESLGALQILARERRRLCPRLFR
                     LGITGSSGKTTTKEIAAAVLGRHAKVALNPGNLNSDIGLSQSMFGVEPDREVGVFEMG
                     MNRKGEIGELAWIYEPDAALVTNVGTAHIGILGSRDAIALEKKAVFSRFCGRQAGLVP
                     EDDDYNAFLKEGVAGEVLDFGPRSTKGFRGAEDLGLDGFAVDWEGLRVRFPLVGRHNL
                     SNAIAAMALAERVGTPPRELAAGLEDARPLFGRSEILRGEVTVIRDCYNANPDSACAA
                     IEFCDSLSWGGRKVYVLGSMLELGAESEAAHRRVGEAAGLSRADALFFFGEEARASSE
                     AAREAGFKGPLFHENDFGRLGESVRASVKAGDLVLLKASRGMALERLAELLAK"
     gene            complement(10341..10631)
                     /locus_tag="PLG14_01450"
     CDS             complement(10341..10631)
                     /locus_tag="PLG14_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOX47821.1"
                     /translation="MKHRAAIALVLSAPLLLVAAVWQAGRYAALAAEARRLEAAQEEW
                     IEQNKKLLASIAVLSSRERAASLAAELGLEKAGPSRRLIVVPSRGNRGGSDG"
     gene            complement(10628..11602)
                     /gene="rsmH"
                     /locus_tag="PLG14_01455"
     CDS             complement(10628..11602)
                     /gene="rsmH"
                     /locus_tag="PLG14_01455"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOX47822.1"
                     /translation="MEVVHVPVMLDEVLSYLVPPREGALMVDCTLGEGGHAEAFLSRY
                     PALRYIGLDADPRIQAKARERLSSFAPRVEFRLGFFDDILESLLSEAEEGRVQRPELV
                     LFDLGISMHHYVEAGRGFGFGKDEALDMRLSPEAPRSAADLVNGEREEELARIIFEYG
                     EERYSRRIAKAIVEARRSGGISTSGRLADVVAGAVPPAYRHGRIHAATRTFQALRIAV
                     NDELGRAERGIRRAAALLAPGGTVAVISFHSLEDRIAKNVFRELAGRSTSAPDAPIGK
                     VPEQAILELLTKKPLEATAEEAARNPASRSAKLRAARRIGPAAGKADL"
     gene            complement(11611..12054)
                     /gene="mraZ"
                     /locus_tag="PLG14_01460"
     CDS             complement(11611..12054)
                     /gene="mraZ"
                     /locus_tag="PLG14_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOX47823.1"
                     /translation="MNLLTGEFRNSLDEKGRVSLPARLRGELPGTSFVLTQGVDKCLW
                     LFPPEQWTALSKKLMDSTSLFQAKARLVQRRILAPAQEVEVDKLGRIPVPQSLREWAG
                     LTKECVILGITKYIEIWDADEYRKYWDDNESEFRAAAEELGELTL"
     gene            12229..12471
                     /locus_tag="PLG14_01465"
     CDS             12229..12471
                     /locus_tag="PLG14_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47824.1"
                     /translation="MTIYEYYVVYPDGDRQEIPGPLEFDALVDLNGRPLPLPLPTAKM
                     IAYRVVKIRRSEERGEAVSLHYVELVPAAELLRYTR"
     gene            12716..13324
                     /locus_tag="PLG14_01470"
     CDS             12716..13324
                     /locus_tag="PLG14_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47825.1"
                     /translation="MDAFLASERPDPRLGLSLVVPIEGIEEGYRALVRRISRIEPGQY
                     YYPPSDLHATVFDFLQARADYSSEAALESAFLDIALEALASERSFAIELRGIVFSSSA
                     GMIKGYDGGALTRIRERIRSLMRLRGLANDERYASESAHVTFTRFRATLEDPAALCAA
                     IGELRESELGEAAATRLELVEHDWYNSAGTRRIIGEIPLEAP"
     gene            complement(13398..15005)
                     /locus_tag="PLG14_01475"
     CDS             complement(13398..15005)
                     /locus_tag="PLG14_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOX47826.1"
                     /translation="MDRRNLVLGVDSSTQSATAVILDRGSFRAVAEAKVRYRDDPRLA
                     RFGLSSGAPVLPPLEEGEASQPPGLFLAALDALLSDLPREALSRLAAIDLSAQQHGQV
                     WLAEGACEALSDLRRPGAGAAGAPSLEERIAPGLAYDRAPIWMSSNTAAEADAIRRAL
                     GGPEAVTGRSGSDSPLRFSGAVLARAARLRPEAYTKASRVHLISSFLAGVLSGNPDAP
                     VDWGNGSGTGLMDWAARAWDGELLAATAAAGGLPGGARGLAARLPPLAHPLALAGRAA
                     AYFTERYGIPEDCAIVVSSGDNPQSKVLASGTLLSLGTSFVVMGEGRRPLPAANAMYD
                     GLGRPFLFGCRTNGSLAWEGLRSAAGLGADDFASAERALASVAPGSVLRIVQPATESF
                     PASPPFDLGATGSFGPDYAGAVDSALGLLALASAEFAGGEGAIAATGGAASSRGVLAR
                     AAAIFGRPVLPIADAGAAAGAAVAAACALVPEAEREGLAERARAVAARPGPAVEPDPA
                     AVRAYRGPGGYLERLAEAFAAAAGRRI"
     gene            complement(15016..16341)
                     /gene="xylA"
                     /locus_tag="PLG14_01480"
     CDS             complement(15016..16341)
                     /gene="xylA"
                     /locus_tag="PLG14_01480"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOX47827.1"
                     /translation="MADLFPRVPHIAYEGPESDNPLAFKYYDPERLVGGKPMKELLRF
                     SMAFWHSMTGKGTDPFGDATMERPWNAASDPMEAARLRVRGVFELTEKLSMPFFCFHD
                     RDIAPEGATLRETNARLDEIVALMKAEMRTSPTRVLWGTANLFSNPRFMHGAATSPEP
                     RVFAYAAAQVKKALEVTKELGGQNYVFWGGREGYETLLNTDMKLELENMARFLRMAAE
                     YADEIGFTGQFLIEPKPKEPTKHQYDTDSQSVYGFLAENGLLSRFKLNIETNHATLAG
                     HTMQHELRFAADRGILGSVDANQGDELLGWDTDQFPTNLYGTTLVMYEILRAGGLGRG
                     GLNFDAKPRRGSFAAEDLAMSHIAGMDAYARGLLSARAMLDAKALENPLAERYSGWTA
                     GLGASIRGGKESMRSLEAQVLDAPSASPADFGLRSGGQERLEALLNRYI"
     gene            16507..17328
                     /locus_tag="PLG14_01485"
     CDS             16507..17328
                     /locus_tag="PLG14_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOX47828.1"
                     /translation="MGFRFDDIVFVYQLRDERQLAWHGRLHSHPAGRYEFHYFISGEG
                     SFRNGDATYSIGAGTLHLSPPGSVHQIVATDSRRPISYYAVLVEAEEDEELSPLLAAL
                     RSARGPRAIGCSHRFFFADLLRKQRSGRRELELSARHNFLAFLYDLAAGGPSAHGSSD
                     NVHVEKALAIMQGSIERSLDLEALCGRLGLSREHFVRLFAERMGMPPMRYFTGLKIEA
                     ARAMLSSTNLRVGEIAEKLGYENQFGFSRAFARVAGLAPSAYRASCLQRADFAAE"
     gene            complement(17470..18165)
                     /locus_tag="PLG14_01490"
     CDS             complement(17470..18165)
                     /locus_tag="PLG14_01490"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOX47829.1"
                     /translation="MDIRNLMEDAVLALVDELFEAEERDRRLGFCTCGQCRLDVVCYV
                     LNRMKPEYIVSGRGLAYSERDAHEKVQRRADIISVAKEGWSRVSHQPRLTADHAARAV
                     QKELPEGPAFNFPTVMGRVFNGLNFEPLAEGDVRLLAEGELMPMVDANWQNPFVLAQA
                     TAGTFIFWPRPKEAAQAGEERRFSFELKASLAGFEPLSHFFELELAADRSARGEFSLQ
                     RVQKIPDLYLFPK"
     gene            complement(18165..19121)
                     /locus_tag="PLG14_01495"
     CDS             complement(18165..19121)
                     /locus_tag="PLG14_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX47830.1"
                     /translation="MSDPGLSPAAPQAAPCPASPPSGGDVLEAALSGRAPPWLERFLA
                     SVGRGAHRHGMIREGDRVLIGVSGGKDSLALALALALRRRRLPFDLELRAVLVDWEPF
                     PAPPGGLAAIGEFFRRLGLPFEVLRADPAAYAQDGPFSCYACARARKRLIFGRAQELG
                     FGTVAFGHHLDDFAETALMNLCFRGRFEPLAPVRVFGLAPEGIGGRAPAEPESDSGDQ
                     AAARGTGADRGSIRVIRPLCEVRESTIRSVAARLGLPVLSVDCPHKDTNLRLSLKPLV
                     AELAKMDTLVRENIYRACFGNPGKAAGEPGLSGRGTARAVED"
     gene            complement(19118..20395)
                     /locus_tag="PLG14_01500"
     CDS             complement(19118..20395)
                     /locus_tag="PLG14_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX47831.1"
                     /translation="MRSNPLVERLASDFTEPVRDPVWGSIYLSPALERLVASPPFAKL
                     SGVRQLGPAHFVYPGATHTRYAHSLGVFHTARRLAAALAARGSLGFVSAEGLSSFLVA
                     ALCHDLGHFPFAHSLKELPLAEHEALGARLLLEEPLRSLAGLAGADPEAAAAIVDPAL
                     PAGGSREIAFFRGALSGVLDPDKLDYLNRDAYFCGVPYGAQDTDYILQRAELGPGDRI
                     GVGEKGLMSVEGLLFAKYLMYRAVYWHKGVRAPTAMVKKAVLLALRDGVLEAEELYGL
                     DDAGFFGLLRARARERGFPPFALAEAVLQGQSYPVLLDLPFDGADPRHRALLGLEARL
                     GFEEELAARISARGPALGPLDLIVDIPEPVSFESDLPVLGEEGEGSLPFSESSTVFSP
                     AVVSGFTRVLRRIRVLARLPSEAAVKAAREALG"
     gene            20452..21294
                     /locus_tag="PLG14_01505"
     CDS             20452..21294
                     /locus_tag="PLG14_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47832.1"
                     /translation="MRKKSNASLVPRRGKASWKGLACLVLAGALAACGPGTARDARRP
                     ARAPSQAQNPPASAVGRPSQAPEPAKIADPAGQAPATGGTGGQSLPGAEPQAPAPAPP
                     ASPSPAEKAKPAIPPSIAQPRAFGLASVAGPVFPEDFAIGPLQDLRPARLDEKAAAEA
                     ATAFLGGLLEGKVAAAVVLPERLALLEAFLAPLFSGERPVSYRLGAIDFSEGPGARDG
                     ASAVAGVALFGAAGAVGRRAAEIGLRRSGAAWYVESFQAAAPGGDGKAGAASVAYEPR
                     LRRP"
     gene            21355..>21608
                     /locus_tag="PLG14_01510"
     CDS             21355..>21608
                     /locus_tag="PLG14_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47833.1"
                     /translation="MATSLKRIEKEFILGAVRDDGVRVLLIAGNGEWPAAITAVDKDH
                     LVLSHSMPQRLLRRGETYEFRFVYREQAMAFRAKLLEAKEA"
BASE COUNT         3199 a         7315 c         8100 g         2994 t
ORIGIN      
        1 cgaggacgcc ctccttctcg gccttcctgg ccgcggaggg tttcctggct cgcgtcgcgc
       61 gcgccggagc cttcccggac ggcgccgcgg acccggcgga cccggcagac gaggccgccg
      121 caggggcctg cgccggccgc ggcgcggctt gggccctcgt cggcgcggcc ggcgcggccg
      181 tgccgagggc cagggcctca ggcttggcca tactggcgag cccccggctc ttcgggcctg
      241 gccgcgaggg ccgcgaggtc gaggccgagc tcggcgcgca gggcctcggc gaggaaggag
      301 ccctcgcccg ctccgtagta gccgggaaag gacccgcggg cgtcgcggtc cgcgaggccg
      361 tcaaggccgg ccgcggggat gccccattcg gcgaggacgc cgcgggcgga ggcgacggcc
      421 agggcgccgt cggacgcgag gccgtcgagg ccggcgctcc tggggcccgc gagcctggac
      481 gcggcgacga agacgtcccg gccctgctcg agggcgaagt cggcggtgat gagggcgccc
      541 gagccggagg gcgcctccac gacgaggatg ccgcggcaca ggcccgagat gacgcggttc
      601 cgctcgggga agctgctctt cctgatctcc gtcccggggg cgaattccga tacgaggagg
      661 ccgcccgagg cgaggatggc cgccgcgagg ccgcggtggg ccgcgggata gaccctctcg
      721 acgccgcagg ggaggacggc gcaggtcggc cccgccagag gcgagcgccg ccgcccctca
      781 agggcgccgc ggtgcgcctc cgcgtcgacg ccgagggcca ggccggagac gacgggcagg
      841 cccgcgaggg agagctcgcg ggagagggag cgggcggcga ggaggccgcg cccggtcggc
      901 cggcgcgtcc cgacgacggc gacggccggc agtccctcgg gcaggcgctc gccgcggaaa
      961 tagaggccga agggggcgcc ggggatctcg cggagggcgg cgggaaagcc ggcctcgtcg
     1021 cgctgcaggt agaaggcgcc catgcgctcg aggaggcggg cgtccgcctc ggccgcggac
     1081 aggagggcct cgggatccca gcgccccgcc ctgaggcggc ggccggcaat cgcctcgagg
     1141 tcggcgcggg agagggaggc gagctcggcg cccgagtcca gggcggcgag gagtcggagc
     1201 ttccccgcgg cgcccatgag ctcgaggcgc tggagggcta ggaggacgat cctccggccc
     1261 gcgtcactcg gcattcatct cccccagtat ccgctcgcgg ttctcctcgg cggccttctt
     1321 cttcgcctcc accctcgtga cgcgggcggc ggtctcgtag acattgaccg cctcctccag
     1381 gcggcccagg cggtagtaga cccggcccag gaggagcatg gcgtccgcgt tcctcgtctc
     1441 gatctcgagg aggcggagga ggagggggac cgagtccgcg ggccgcccga gctcgaactc
     1501 gtagaggacg gccaggccgt agagggccgc gccgtagcgg gggttgatgt ctatggccgc
     1561 ccggtagctc gactccgacg tgaggagcca cttccgcctc tcgggctccc cgtcggggcc
     1621 cagggcgagc ttggacttgg cgatgtagcc ggcggcgatg cccgcgttgt agtagaggtt
     1681 cgagttggag gggaagtact cgacggcctt ggcgagggcc tcgtaggcct cgccgtaaag
     1741 cttcttctcg aggagccggg aggcgaggat cttgtagtag atgccgacct tctcgtccgc
     1801 gaggacgacg cgctcggcct ccttcgagta cttggcgatg ccctccttga gctcctcgac
     1861 gctctgggga ggggctccca cagcgctcct gtcctccagg tcgaggaggc gcttgagatc
     1921 gccgtcgggg ccgcaggacg agaggaggcc gagggcggcg agggcgagga ggacgcggac
     1981 cgcgccgccg gcgcggcggc ggccggcggc gccccggggg cggccgcgct cagttccgac
     2041 tgcttgcctt ggatccatgc cgccttcctc cttcctagaa cgtctccgcc ccgcccttcc
     2101 gcttgcgcgg gcggagccta tttccgcggg aaatactcgc ggtggtagtc gccgagggcc
     2161 tcggagtcga tgtgcgtgta gatctgcgtc gtcgagatgt cggaatgccc gaggagctcc
     2221 tggacggtgc gcaggtcggc cccccccgcg aggaggtggg tcgcgaagct gtgcctgaag
     2281 gtgtggacct tcgcgtcgac gccggcctcc tgccggacga ggtcgaagcg cttccagatg
     2341 cccttccgcg aaaggccctt ccccgcgtgg ttcaggaaga cccggtcgct ccgcccgccc
     2401 ttgaggaggg ccggccggcc ttcctccagg tagcgccgga gccaggccgc ggcctcgtcg
     2461 ccgaagggga cgaggcgctc cttcttgcgc ttgccgagga ccctgatcac cttctcgtcc
     2521 aggaagaggc cgtcgaaggt cagggctgag gcctccgaga tccggaggcc gcaggagtag
     2581 atgagctcga agagggcccg gtccctgagc ccgcgggggc tgcctgagtc gatcgccgcc
     2641 aggaatcggt cgatctcctc cagcgcgagg acggccggga gctgccgctc ctgcttgggc
     2701 gtctcgatga gctcggaggg atcgtcctcg cggacgccct ccagcctcag gaacttgaag
     2761 aaggagtgta cgctcgagac gacgcgggcc atggtctttc gggaaagcga ggagctttgc
     2821 tcctcgcttt cccggcgcgg agctccttgc gtcgctccgc gatcccctgg agagccttgc
     2881 tcctcgcttt cccggcgcgg agctccttgc gtcgctccgc gatccgctgg agagccttgc
     2941 tcctcgctct cgcgctcgcg gagggcggcg gcgccggact ggcgccagac gaggaagccg
     3001 aggacgtcgg agctcgtcgc cgtcgccggg tctaggcccc gctcggcgag ccaggactcg
     3061 agatgggagg cctcgcggag gtagacctcc gcggtcagcg gggaacggcg gcgggccgcg
     3121 agaagatagg ccctgtagcg ccggaggagg tcgtccacct tcaggcctcg ctcttctccc
     3181 cggccaggcg gcggtcgcgc aggaccgtcg ggatgtcgag ctcgtcctcg ccctcgttgc
     3241 ggctcaggag gtactgcttc gccctctggg aggggttctc cgtgatgcgc ttccactcgt
     3301 cgctggagat gtagtcctcg aggccgcggc gggaggcctc gcggacggag gcggaggcgg
     3361 ccgtgttctg ggtcttgagg ctccggttga agccggtggc gatcaccgtg acgcggacct
     3421 cgtcgcccag ggcgggatcg gtgacgatgc cggggatcac gagggcctcg gggtcggcgc
     3481 tctcggtgat gaggttgacg atctcctcgt actcggtgag ggcaaggtca tcgccgcccg
     3541 tcacgttgac gaggatgttg cgggcgccct cgatgcgcgc gtcctcgagg agggggttgt
     3601 tgaccgcctt gcccgcggac tcgaccgcgc ggttgtcgcc ggaggccgcg ccgatgccca
     3661 tgatggcgtc gcccatgccc tccatcaccg tccgcacgtc ggcgaagtcg atgttgatgt
     3721 cgcccgggat ggtgatgagg tcggagatgc cctggacgcc ctggcgcagg acgtcgtcgg
     3781 cgagctggaa ggcctccttg atcggcgtgc gccgctcgac gaccttgagg aggtgctggt
     3841 tcgggatgac gatgagggtg tccacggcct ggcggagctt ctcgatgccc tcctccgcga
     3901 ggcgggcctt cacccgtccc tcgaagtcga agggccgagt gacgacgccg acggtgaggg
     3961 cgcccaggtc gcgggcgacc tgggcgatca ccggcgcgga gccagtgccc gtgccgccgc
     4021 ccatgccggc ggtgacgaag accatgtcgg cgcccttgag cgcctgggct atcgcgtcgc
     4081 ggtcctcgag ggcggccttc tcgccgatct cgggcttccc tccggcgccc aggccgtggg
     4141 tcaccttggc cccgaggccg agcttcacct cggcccgcga gaggttcagg gcctggaggt
     4201 cggtgttcgc gacgatgaac tggacgttcc tgaggcccgc cacgatcatg cggttgaccg
     4261 cgttggagcc tccgccgccc gccccgatca ccttgatgac ggtcggactc gcgtccgcgc
     4321 ccctgtcctc cacgagttcg atgttcatgg tcccctcccc tttcgtcatt ccgccgtatc
     4381 gagggtgcgc ggcgccttcc gtccccacgg cggcgccgcc ccctgaatct ttgctagaag
     4441 aatccgttct tgagccagtc gaagatcttc cccaggctcg ggcccttcgc cttctccgtc
     4501 ctggcggcct cggcgcgcct ttcgggcgca atcgactcgg cgcccaggag gacgaggccg
     4561 acgccggtcg cgtagaccgg gctccggtac tcctccacga ggccgccgac cgtgatgggg
     4621 ttccccaccc gcaccggcat ctcgaagatc tcctgcgcga gctcggccgc gccctgcatg
     4681 agggcgccgc cgcccgtgag gacggccccg cccttgatgt ggcgccagtg ccccatcttc
     4741 tccacgcgct cgcgggccat gcggaggatc tcctccatgc gcggctgggc gatcgtgcag
     4801 agcttcctcc gcgagatctc cacgggaggg cggccgccga cgcccgggac gacgacggac
     4861 tcttcggggt cgaccccctc gagccaggcg gaggcggcct cgcgcttcag gcgctcggcc
     4921 gcgtccatgg gcacggagag catgatcgag aggtcgttcg taacctgcgc gccgcctacg
     4981 ggtatcacgc tcgtgaagta gggcgcgccg tccgagtaga cgaggaggtc ggtggtcccg
     5041 gcccccaggt cgacgaggag gcagccgagc tccttctcgt cggaggtgag ggtcgcgcgc
     5101 gccgcggcca ggctctggag gatgaggcgc tcgaccttga agccggcgcg gttcacgcac
     5161 ttcacgaggt tctgcgccgt cgtgacggag cccgtgatga tgtggacctc cgcctccagg
     5221 cgcaccccga tcatgtcgag ggggttcctg atgcccttct ggtcgtcgac gatgaaggtc
     5281 tgcgggatca cgtgcaggac ctcgcggtcc atcgggatca cgacggcgcg ggccgcctcg
     5341 atgacgcggt ccacgtccga ctgggtgatc tcgcggccct tgccggtgac ggcgacgacg
     5401 ccgcgggagt tgatgccctc gatgttggcg ccggcgacgc cgacgaagca gtccttcgcc
     5461 tcgcggccgg acatgagctc ggccgcctct atggcggcgg cgacggaggc cagggtcgcc
     5521 tcgatgttga cgacgacgcc gcggcggagg cccgtggagg gggccgtgcc gacgccggtg
     5581 atctcgagga ccccgttctc gttgtattcc ccgatgacgg ccctggtctg ggcggtcccg
     5641 acatcgaggc cgacgataag gttgtcagcc agactgtcct cccttgctgc ggtagacgac
     5701 cgttcccctg cggaaatcga tctcctcgac gccgtccgcg aggccgcggg agccgagcac
     5761 gtcgaggacg agtatgatag agcggagcgt cgcctcgttc aagacggcgc cggtgcgcac
     5821 ggggacgcgg tggtgcagcg ggtagaggac gagctcgggc tcgccgtact tgggcttcgc
     5881 gatcctgatc tcggagaagg ccgcgaggag ggagggcgcc ctggcctcga gctccccgag
     5941 ggaggcgagg aggggcacga ggctcggcgg caggcgcgtc ccgaggctga agccctcgaa
     6001 gcggaggccg gagacgaggg gaaggtcggc gaggccggcg ggcgggaggc ccgaggcgcc
     6061 cgcgagctcg gaggcgaagg cgaaggccac gccctcgccg tcgacgagga cggtctccag
     6121 gcggccgttc cgctccgcga ggacggcggc gacggcctgg cgctcggcga cctcgatgcg
     6181 gaggccgttc gggaagaggc ggcggacgcg ggccccggct atcctcggcg aggcgaggag
     6241 ggcggcgcgg acgcgctcgg ggtcgagggc gaagaagtac tccgagccgt ggaccagggc
     6301 ggcgtccagg acctcgcggt cgctcatcgt ggcggcgccc acgacctcgt agcggctcac
     6361 gcggagggcc ggcgggacga ggacgaaggc cgccgccgcc gcgagggcga gggcagccgc
     6421 gcccaggagg aggccgaggg cccgctccac ggaggcgcgg cgccccgcgc cgcggccgcg
     6481 ggccggggcg gtcccgaggc cctcggcccc gcggatctcc ggcctggggc ggcggcggcc
     6541 ggcgtcgttc ccgcgggcgc ccgcggcctc gaagctgacg cggccgagcg cgaactcaga
     6601 catggcggcc tccctcggag cgcgagagat tggccaggac gccgcaggtc gcggcggtgg
     6661 ccagcaagga ggagccgccc gcggagaaga aaggcagggc gatgccggtg gcgggcatcg
     6721 cgccggcggc gacggcgacg ttcacgagga cctcgaggag gaggagggag gccaggccga
     6781 agccgaggta ggagcggaag ccgtcctcct cccggaagga ggaccggaag gccctgaagg
     6841 cgaagaagcc ccagaggccg aagaaggcga gcactcccag gaagccggtc tcctcggcca
     6901 gggccgcgaa gatgaagtcg gactggacat aggggaggct gccgagcttg agggtcccca
     6961 ggccgaggcc cttgccgaag aagcctccgg acctgatggc ccgcagggag cccaggacct
     7021 ggtagccctg gccgtggggc tcgtaggccg ggaagaggaa ggcgaggatg cggcggaggc
     7081 ggaagtcgct cgtgaggacg gagagggccg cgaggggccc gcccacggcg gccaagcccg
     7141 cgaagaaggc cagcggggcg ccggccaccc agaagacggc gacggcggcg gcggccgcta
     7201 tgacggccgt ggagaagtcg ttctgcagga agacaaggag gcagcccgag cccgcgagga
     7261 ggaagggcgg catgagcggc gcgacgccca gggccttcgc ctcctgccgc ttcgccagga
     7321 cgtgggcgag atagaagacg gagaccggct tccagaactc ggagggctgt atcgtcgtga
     7381 agccgaggtc gatccagcgc gaagcgccgt tgcggttctc ccccaggccc gggatgaagg
     7441 gcaggaggag ggagcccagg ccgaggaggg tgacgaggcc ggccttcttc ctgaggaggt
     7501 cgaggggcgc gagggctccg gcccagaaga ggccgacgct cggcgcgagg aagaggagct
     7561 gccggagggc gaagtggtag ggcgccttcc cgatggagag ggcgtagccc gacgaggccg
     7621 accagagcgc ggcgaagccc gtccccgcga ggaggaagag ggaggcgagg agggcgctgt
     7681 cccaggtcct ttcccgcgcc atgggctcga cggcgaatcc gctcggcatc ttttatgtcc
     7741 ctactgtatc ttgagggtgg agagggcgat gatcgcgaaa atcccgccga gtatccagaa
     7801 gcgggtcacg accttcgtct ccttccatcc cttgagctcg aagtggtggt ggatgggcgc
     7861 catcttgaag aggcgcttcc ccttggagag cttgaaccag ccgacctgga gggtcacgga
     7921 ggcgacctcc atcacgaaga ccccgccgat gaggaggagc aggatctcct tcttcacgat
     7981 aagggagagg acgccgagga cgccgcccag ggccagggag ccgacgtcgc ccatgaagac
     8041 ctccgcggga tggccgttga accagaggaa gcccacgcag gcgccgatga gggccaggtt
     8101 gaagaccgtg agctcgccag cccccttgag gaaggggatg gagaggtagg cggaccagtc
     8161 ggcccggccc gtcacgtagg tcaggatcgc gaaggcgatg agggcgatga tcgtgagccc
     8221 cgtcgcgagg ccgtccaggc cgtccgcgag gttgaccgcg ttggaccacc cgaccatgta
     8281 gacgaccgcg aaggggatcc agagcgcgcc caggtccagg acggggtact tgaagaaggg
     8341 cacgtagagg agggtcgtct gcgggttgcc gtcgaagtag aggacgaggg ccgcggccga
     8401 ggcgatcacg acctgcgacc agaatttcgc cgcggcgggc aggccgtcgg agttccggta
     8461 cttgatcttc cgccagtcgt ccaggaagcc gatgaggccg aagccgagga gggagcccag
     8521 ggccacccag gtgtagaggt tccgcaggtc gctccacagg aggacggcgg aggcgacggc
     8581 gaggatgatg aggatgccgc ccatcgtcgg cgtccccgac ttggcgaggt ggctctcggg
     8641 gccgtcggag cgcaccgcct ggccgaactt gaggacccgc agcttctcga tgagcctggg
     8701 cccgaagacg aaggcgatga ggagggcggt gaccgcggcg taggcggagc ggaaggtgag
     8761 gtacttgaag acgttgaggg gcgagaagta cttcaccagc ggatacagga gctctaggag
     8821 catgaggctt cctccgtcac ttcgccaaca gctcggcgag gcgctcgagg gccatgcccc
     8881 gcgaggcctt gaggaggacc aggtcccccg ccttgaccga cgcccggact gactcgccca
     8941 gccggccgaa atcgttctcg tggaagaggg ggcccttgaa gcccgcctcc ctggccgcct
     9001 cgctggacgc ccgcgcctcc tccccgaaga agaagagcgc gtccgcccgc gagagccccg
     9061 cggcctcgcc caccctgcgg tgtgcggcct cggactccgc cccgagctcg agcatcgagc
     9121 cgaggacgta gaccttccgg ccgccccagg agagcgagtc gcagaattcg atggccgcgc
     9181 aggcggaatc cgggttcgcg ttgtagcagt cgcggatcac ggtgacctcg ccccggagga
     9241 tctccgaacg gccgaagagg gggcgcgcgt cctcgagccc cgcggccagc tcccgcggag
     9301 gcgtccccac gcgctccgcg agagccatcg ccgcgatggc gttcgagaga ttgtgccgcc
     9361 ccacgagggg aaagcggacc cggagcccct cccagtcgac ggcgaagccg tccagcccca
     9421 ggtcctccgc cccgcggaag ccctttgtcg agcgcgggcc gaagtccagg acctcgcccg
     9481 cgactccttc cttcaggaag gcgttgtagt cgtcgtcctc gggcacaagg ccggcctggc
     9541 ggccgcagaa gcgcgagaag acggcttttt tctctaaagc gatggcgtcg cgagatccga
     9601 gaatgccgat atgggcagtc ccgacgttcg tgaccaaggc ggcgtcgggc tcgtagatcc
     9661 aggccagctc gcctatctcg cccttcctgt tcatgcccat ctcgaagacg ccgacctcgc
     9721 ggtccggctc gacgccgaac atgctctgcg agaggccgat gtccgagttg aggttgccgg
     9781 ggttcagggc caccttggcg tggcggccca ggacggctgc ggctatctcc ttggtcgtcg
     9841 tcttcccgct cgagcccgtg atgccgagcc ggaagagccg ggggcagagc ctccgccgct
     9901 cgcgggcgag gatctgcagg gccccgaggc tctcggggac gaggaggacg gccgcgccct
     9961 tccgcgcggc gaggggcgcg agggcggagg cccgcgcctt ccagccccgc tcctccgcga
    10021 ggacgcagga agcccctgcc tccagggcct gggcgatgta gtcgtggccg tccgccttct
    10081 ctccgggaag ggcgacgaag agcgagccgg gcccggcctt gcgggaatcg acggccacct
    10141 cgaagaagcg ggcggcggcc gggccttcaa ggctcgaggc ggcgaaggcc gcggctatgc
    10201 ccgaggcgtc gaggccggac aggccggccg aggcgccggg cgccggggcc aggccagcgc
    10261 gggagagggc gacgggcgcg tactccgcgg cggctccgcg gaggatcgcg agggcctcgc
    10321 cggccgagaa ggagaggcgc tcagccatcg gagcctccgc ggttgccgcg ggacgggacg
    10381 acgatgaggc ggcgcgaggg gcccgccttc tccaggccga gctcggctgc cagggaggcc
    10441 gcgcgctcgc ggctcgagag gacggctatg gaggccagga gcttcttgtt ctgctcgatc
    10501 cactcctcct gggcggcctc caggcggcgg gcctcggcgg cgagggcggc gtagcggccc
    10561 gcctgccaga cggcggcgac gaggaggagg ggggcggaga ggacgagggc gatcgcggcc
    10621 cggtgcttca taggtcggcc tttcccgctg ccggcccgat cctgcgggcc gcgcggagct
    10681 tcgcgctccg ggaggcgggg ttgcgggcgg cctcttcggc cgtcgcctcg agcggcttct
    10741 tggtcagaag ctcgaggatc gcctgctccg gcactttacc tatcggcgcg tccggggccg
    10801 aagtggagcg gccggcgagc tcgcggaaga cgttcttcgc gatcctgtcc tcgagggagt
    10861 ggaagctgat caccgcgacg gtcccgccgg gggccaggag ggcggcggcg cggcggatgc
    10921 cgcgctcggc ccggccgagc tcgtcgttca ccgcgatgcg cagggcctgg aaggtcctgg
    10981 tcgcggcgtg gatgcggccg tggcggtagg ccgggggtac ggcgccggcg acgacgtcgg
    11041 ccaggcggcc gctcgtggag atgccgcccg agcgcctcgc ctcgactatc gccttggcga
    11101 tgcggcggga atagcgctcc tcgccgtact cgaagatgat cctggcgagc tcttcctcgc
    11161 gctcgccgtt cacgaggtcg gcggcgctcc gcggcgcctc cggggagagg cgcatgtcca
    11221 gggcctcgtc cttgccgaag ccgaagccgc ggccggcctc tacgtagtgg tgcatggaga
    11281 tgccgaggtc gaagaggacg agttcggggc gctggaccct gccctcctcc gcctcggaga
    11341 ggagggactc gaggatgtcg tcgaagaagc ccaggcggaa ctcgacccgg ggcgcgaagg
    11401 aggagaggcg ctccctggcc ttggcctgga tgcgggggtc ggcgtcgagg ccgatgtagc
    11461 ggagggcggg atagcgggag aggaaggcct ccgcgtggcc gccctccccg agggtgcagt
    11521 cgaccatgag ggcgccttcg cgcggcggaa cgaggtaaga gaggacctcg tccagcatga
    11581 ccggcacatg cactacttcc atacgtccgc ctagagcgtg agctccccca actcctcggc
    11641 cgcggcccgg aactcggact cgttgtcgtc ccagtacttc cggtactcgt cggcgtccca
    11701 gatctcgatg tacttcgtga tcccgaggat gacgcattcc ttggtcaacc ccgcccattc
    11761 ccgcaggctc tgcgggacgg ggatcctgcc cagcttgtcg acctccactt cctgggccgg
    11821 cgcgaggatg cgccgttgca cgaggcgcgc cttggcctgg aagaggctcg tcgaatccat
    11881 gagcttcttg gagagagcag tccattgctc aggcgggaaa agccagaggc atttgtccac
    11941 tccctgggtc aggacgaagc tagttcccgg cagttccccg cggagtcgcg cgggaaggga
    12001 aactcgtcct ttttcgtcca ggctgttcct gaactcccca gtcagcagat tcatgccact
    12061 acgtcctact ttttaccact ttttcccacc acgatcttat actacttcgc ggaggacagc
    12121 tgtcaacaaa agcgaattcg aaaagaggag acagaaacaa tatttttatc cattaggctt
    12181 cacgcctgta tagctgtatc ccgggagcgc ctggactatg ctcccggcat gaccatctac
    12241 gagtactatg tcgtttatcc ggatggagat cggcaggaaa taccgggtcc gctcgagttc
    12301 gacgccctcg tggacctgaa cggacggccc ctgcccctcc ccctgccaac ggccaagatg
    12361 atcgcctaca gggtcgtgaa gatcaggagg agcgaggaga ggggtgaggc ggtgtccctc
    12421 cactacgtcg agctcgtgcc cgcggcggag ttgctgcgct ataccagatg attcatattt
    12481 gcaaatattg catttcgcaa tatttgcgga ttccctgcgc tattgagccg tccctcgggc
    12541 atttcgtccc ctcgacccgc cgaagggatt cccttcgcag tcttcagccc cgggatggac
    12601 gcagccgccg cgccgacggg ggagccagac ccgggaggaa gccctgagcg gcccggaact
    12661 atacgaccgg atccacgcgt cgaacatcgg ccgcgtcagg tcagggaaca tcgaggtgga
    12721 cgccttcctt gcctcggaaa ggcccgaccc gagactgggc ctgtcgctgg tcgtcccgat
    12781 cgagggaatc gaggagggct atcgggccct tgtccgccgg atctcccgga tcgagccagg
    12841 gcagtactac tacccgccga gcgacctcca cgccaccgtc ttcgatttcc tccaagccag
    12901 ggcggactac tcgagcgaag ccgcgctcga aagcgctttc ctcgacattg cgctggaggc
    12961 cctcgcgtcg gagagaagct tcgccatcga attgcgcggc atcgtgttca gctcgtcggc
    13021 cgggatgatc aagggctacg acggcggcgc cctgactcgg atccgggaga gaatcaggag
    13081 cctcatgagg ctacgggggc tcgcgaacga cgaacgctac gcctctgagt cggcgcacgt
    13141 caccttcacg agattccgcg ccaccctgga ggacccggcc gcactgtgcg cggcgatcgg
    13201 cgagcttcgc gaatcagagc tgggcgaagc ggccgcgacg aggctggagc tcgtcgagca
    13261 cgactggtac aactccgcgg gaacaaggcg gatcatcgga gaaatccccc tcgaggcccc
    13321 ctgacggacg gcttcgtgcc gccggcgtct tgccgccggc ggccttccgt cactgccccg
    13381 cggccccccg ggcccgccta gatcctcctg ccagccgccg ccgcgaaggc ctcggcgagc
    13441 cgctccaggt agccgcccgg gccgcggtag gccctgaccg ccgcgggatc gggctcgacc
    13501 gccgggccgg gccgggccgc gacggcgcgg gcccgctcgg ccaagccctc gcgctcggcc
    13561 tcggggacaa gggcgcaggc cgcggcgacc gcggcgcccg cggcggcccc ggcgtcggct
    13621 atcgggagga cggggcggcc gaagatggcc gcggcccgcg caaggacgcc ccggctcgag
    13681 gccgcgccgc ccgtggccgc gatcgcgccc tcccctcccg cgaactccgc ggaggccagg
    13741 gcgaggaggc ccagggccga gtcgacggcg cccgcgtagt ccggtccgaa ggagcccgtc
    13801 gcgcccaggt cgaagggcgg cgaggcgggg aagctctccg tggccggctg gacgatcctg
    13861 aggacggagc ccggcgcgac ggaggcgagg gcgcgctcgg ctgaggcgaa gtcgtcggcg
    13921 ccgaggcccg ccgccgagcg gaggccctcc caggccaggg agccgttcgt cctgcagccg
    13981 aagaggaagg gcctccccag gccgtcgtac atcgcgttcg cggccggcag cggcctcctc
    14041 ccctccccca tcacgacgaa gctcgtgccc agggagagaa gggtcccgga ggcgaggacc
    14101 ttgctctggg gattgtcgcc ggaggagacg acgatggcgc agtcctcggg gatgccgtag
    14161 cgctccgtga agtaggcggc ggcccgcccg gccagcgcga ggggatgggc gaggggcgga
    14221 agcctcgcgg ccaggccccg ggcgccgccg ggcaggccgc cggccgcggc cgtcgcggcc
    14281 aggagctccc cgtcccaggc ccgggccgcc cagtccatga ggccggtccc cgagccgttg
    14341 ccccagtcga cgggcgcgtc ggggttcccc gagaggacgc ccgcgaggaa ggacgagatg
    14401 aggtggaccc gcgaggcctt cgtataggcc tcgggcctga gccgcgcggc ccgggccagg
    14461 acggccccgg agaagcgcaa tggactgtcc gagcccgagc gcccggtgac ggcctcaggc
    14521 ccgcccaggg cccgccggat cgcgtcggcc tcggccgccg tgttggagct catccagatc
    14581 ggcgcacggt cgtaggcgag gccgggggcg atccgctcct cgagggaggg cgcgccggcc
    14641 gcgcccgcgc cgggccggcg caggtccgag agggcctcgc aggccccttc cgcgagccag
    14701 acctggccgt gctgctgggc cgagaggtcg atcgccgcga gccgcgacag ggcctcgcgg
    14761 ggcaggtcgg agaggagggc gtccagggcc gcgaggaaga gccccggcgg ctgcgaggcc
    14821 tccccctcct caaggggtgg caggaccggc gcgccggagg agaggccgaa gcgcgcgagc
    14881 cgcgggtcgt cgcggtagcg gaccttcgcc tcggcgacgg cccggaagct gccgcggtcc
    14941 aggatcacgg ccgtcgcgct ctgggtgctc gagtcgacgc cgaggacgag gttccgcctg
    15001 tccacgatgc ccctcctaga tgtagcggtt gaggagggcc tcgaggcgct cctggccgcc
    15061 cgagcggagg ccgaagtcgg cgggcgaggc agagggcgcg tccaggacct gcgcctccag
    15121 gctcctcatg ctctccttcc cgccccggat cgaggcgccg aggcccgccg tccagcccga
    15181 gtagcgctcc gcgaggggat tctccagcgc cttcgcgtcc agcatcgccc gggctgagag
    15241 gaggccgcgc gcgtaggcgt ccatgcccgc gatgtgcgac atcgcgaggt cctccgccgc
    15301 gaagctgccg cggcggggct tcgcgtcgaa gttgaggccg ccccggccca ggcccccggc
    15361 gcgcaggatc tcgtacatca cgagggtcgt gccgtagagg ttcgtcggga actggtccgt
    15421 gtcccagccc aggagctcgt cgccctggtt cgcgtccacc gagccgagga tgccgcggtc
    15481 ggccgcgaag cggagctcgt gctgcatcgt gtggcccgcg agggtcgcgt ggttcgtctc
    15541 gatgttgagc ttgaagcggg agaggaggcc gttctccgcg aggaagccgt agaccgactg
    15601 ggagtccgtg tcgtactggt gcttggtcgg ctccttgggc ttgggctcga tgaggaactg
    15661 gccggtgaag ccgatctcgt cggcgtactc ggcggccatc ctgaggaagc gggccatgtt
    15721 ctcgagctcg agcttcatgt cggtgttgag gagggtctcg tagccctcgc ggccgcccca
    15781 gaagacgtag ttctggccgc cgagctcctt cgtcacctcc agggccttct tgacctgcgc
    15841 ggccgcgtag gcgaagacgc ggggctcggg gctcgtcgcg gcgccgtgca tgaagcgggg
    15901 gttcgagaag aggttggccg tgccccagag gacccgggtc gggctcgtcc gcatctcggc
    15961 cttcatgagg gcgacgatct cgtcgagcct ggcgttggtc tcgcgcaggg tcgccccctc
    16021 gggggcgatg tcgcggtcgt ggaagcagaa gaagggcatg gagagcttct ccgtgagctc
    16081 gaagacgccg cggacgcgga ggcgcgcggc ctccatcggg tcggaggcgg cgttccaggg
    16141 ccgctccatc gtcgcgtcgc cgaagggatc ggtgcccttg cccgtcatgg agtgccagaa
    16201 ggccatggag aagcgcagga gctccttcat gggcttgccg ccgacgagcc tctccgggtc
    16261 gtagtacttg aaggcgaggg gattgtcgga ctcggggccc tcgtaggcta tatggggaac
    16321 gcggggaaag agatctgcca tctggtgcct ccttggcttc cttaagggta gttccgcccg
    16381 ggggatcggg caatcccgcc ctttgacttt ctatacccga aattgatatt ctaaggtcct
    16441 ggcaccaggc ctgcgggccg ccggcggccg taaggccggc ccgcggctcg ggaaggagac
    16501 cctagggtgg gcttccgctt cgacgacatc gtcttcgtct accagctcag ggacgagcgc
    16561 cagctcgcct ggcacgggcg gctccacagc caccccgcgg gacgctacga gttccactac
    16621 ttcatctcgg gcgaggggag cttcaggaac ggggacgcga cctactccat cggcgcggga
    16681 accctccatt tgagcccgcc cggctcggtc caccagatcg tcgcgaccga ctcccggagg
    16741 cccatcagct actacgccgt cctcgtggag gccgaggagg acgaggagct ctcccccctc
    16801 ctcgcggccc tccgctcggc ccgcggcccg cgcgccatcg gctgctccca ccgcttcttc
    16861 ttcgccgacc tcctgcggaa gcagcgctcg gggaggcgcg agctcgagct atcggccagg
    16921 cacaacttcc tcgccttcct ctacgacctc gcggcgggcg ggccgtccgc ccacggcagc
    16981 tccgacaacg tccacgtgga gaaggccctc gccatcatgc agggctcgat cgagcggagc
    17041 ctggacctcg aagccctctg cgggcggctc ggcctctccc gcgagcactt cgtccgcctc
    17101 ttcgccgagc gcatgggcat gccgcccatg cgctacttca ccgggctcaa gatcgaggcg
    17161 gccagggcca tgctctccag cacgaacctc cgcgtgggcg agatagccga aaagctcggc
    17221 tacgagaacc agttcggctt ctcgcgggcc ttcgcccgcg tcgcgggcct cgcgcccagc
    17281 gcctaccgag cctcctgcct ccagcgcgcg gacttcgcgg cggagtaggg gcgcgcggag
    17341 cgacgggcgg cgccggacgg agcgccgccg gcggcggctt ggcccgcgcg gcgcgccggg
    17401 ttctagacgg tgcaggcggc caggacgtgc cggcgcgggc aggtcgcgcg gaggtttgcc
    17461 gcggcccgcc tatttcggga agagatagag gtcggggatc ttctggacgc gctggaggga
    17521 gaactcaccc cgcgccgatc ggtcggccgc gagctcgagc tcgaagaagt gcgagagggg
    17581 ctcgaagccg gccaggctgg ccttgagctc gaaggagaag cgccgctcct cccccgcctg
    17641 ggcggcctcc ttgggacggg gccagaagat gaaggtgccc gccgtcgcct gggccaggac
    17701 gaaggggttc tgccagttcg cgtccaccat cggcatgagc tcgccctcgg cgaggagccg
    17761 cacgtccccc tcggccaggg gctcgaagtt gaggccgttg aagacccggc ccatgacggt
    17821 cgggaagttg aaggccggcc cctcggggag ctccttctgg accgcccggg ccgcgtggtc
    17881 ggccgtgagg cggggctggt gcgagaccct cgaccagccc tccttcgcga cggagatgat
    17941 gtcggctcgg cgctggacct tctcgtgggc gtcgcgctcg ctgtaggcca ggccccgtcc
    18001 cgagacgatg tactcgggct tcatgcggtt gaggacgtag cagacgacgt cgaggcggca
    18061 ctggccgcag gtgcagaagc ccaggcgccg gtcgcgctcc tccgcctcga aaagctcgtc
    18121 gaccagggcg aggacggcgt cctccatcag gttgcgaatg tccatcagtc ctccacggcg
    18181 cgagcggtcc cgcgccccga caagcccggc tcccccgcgg ccttccccgg gttcccgaag
    18241 caggcgcggt agatgttctc ccgtactagt gtatccatct tcgcgagttc cgcaacgagg
    18301 ggcttgaggc tcagcctgag attcgtgtcc ttgtggggac agtcgacgga gaggacgggc
    18361 aggccgagcc gggcggccac gctccggatc gtcgactccc tgacctcgca gaggggcctg
    18421 atgacgcgga tcgagcccct gtccgccccc gttccgcggg cagccgcctg gtcgcctgag
    18481 tcggactcag gctcggccgg ggcgcggccc ccgatccctt ccggcgccag cccgaagacg
    18541 cgcaccggcg cgaggggctc gaagcggccg cggaaacaga ggttcatgag ggcggtctcc
    18601 gcgaaatcgt ccaggtggtg gccgaaggcg accgtcccga agcctagctc ctgggccctg
    18661 ccaaaaatga ggcgcttgcg cgccctggcg caggcgtagc aggaaaaggg cccgtcctgg
    18721 gcgtaggcgg cgggatcggc cctgaggacc tcgaagggaa ggccgaggcg gcggaagaac
    18781 tcgcctatcg cggccaggcc cccgggcggc gcgggaaagg gctcccagtc cacgaggacg
    18841 gcgcggagct cgaggtcgaa gggaaggcgc cgccgcctca gggccagcgc gagcgccagg
    18901 gcgagggagt ccttgccgcc cgagacgccg atgaggacgc ggtcgccctc ccggatcatc
    18961 ccgtggcggt gcgcgccccg gccgacggag gcgagaaagc gctcgagcca gggcggcgcc
    19021 ctgcccgaga gcgcggcctc gaggacatcg ccccccgagg gcggcgaggc ggggcagggc
    19081 gcggcctgcg gcgcggccgg ggaaaggccc ggatcgctca tcccaaggcc tcgcgcgccg
    19141 ccttgacggc ggcttccgag ggcagtctcg cgaggacgcg gatgcgccgg aggacccgcg
    19201 tgaagcccga gacgacggcc ggcgagaaga cggtcgagct ctcggagaag ggaaggctcc
    19261 cctccccctc ctcgcccagg acggggaggt ccgactcgaa ggagacgggc tccggtatgt
    19321 cgacgatgag gtcgaggggg ccgagggccg ggcccctcgc ggagatcctg gccgcgagct
    19381 cctcctcgaa gccgagcctc gcctccaggc cgaggagggc ccggtgccgg ggatcggccc
    19441 cgtcgaaggg aaggtcgagg aggacggggt agctttgccc ctgaaggacg gcctccgcga
    19501 gggcgaaggg cgggaagccg cgctcccggg cccgcgcgcg caggaggccg aagaagcccg
    19561 cgtcgtcgag gccgtagagt tcctcggcct cgaggacccc gtcgcgcaag gcgaggagga
    19621 cggccttctt caccatggcg gtcggcgcgc ggacgccctt gtgccagtag accgcgcggt
    19681 acatgaggta cttcgcgaag aggaggccct ccacgctcat gaggcccttc tccccgacgc
    19741 cgatccggtc tcccggcccg agctcggcgc gctggaggat gtagtccgtg tcctgggctc
    19801 cgtaggggac gccgcagaag taggcgtcgc ggttcaggta gtcgagcttg tccgggtcga
    19861 ggaccccgga gagggcgccg cggaagaagg cgatctcccg gctgcccccc gcgggcaggg
    19921 cgggatcgac tatcgcggcg gcggcctcgg gatcggcccc cgcgaggccg gccagggagc
    19981 gcagcggctc ctcgaggagg aggcgggcgc ccagggcctc gtgctcggcc aggggcagct
    20041 ccttcaggga gtgggcgaag ggaaagtggc ctaggtcgtg gcagagggcg gcgaccagga
    20101 aggaggagag gccctccgcg gagacgaagc cgagggagcc cctggcggcg agggccgcgg
    20161 cgaggcgccg ggccgtgtgg aagacgccca gggagtgcgc gtatcgcgtg tgcgtcgcgc
    20221 cgggatagac gaaatgggcg gggccgagct gccgcacgcc cgagagcttc gcgaaggggg
    20281 gagatgcgac gaggcgctcc agggcgggag agaggtagat gctgccccag accgggtccc
    20341 gcaccggctc ggtgaagtcc gaggcgaggc gctcgactaa ggggttcgat ctcaaggcgg
    20401 gcctcccgcg atttgaccct gcggcctgaa tgtagtagaa atgtacacgt aatgcggaaa
    20461 aagtccaacg cctcccttgt cccgcgccgc ggaaaggctt cctggaaggg cctcgcctgc
    20521 ctggtcctgg ccggggccct cgccgcctgc ggccccggga cggcccggga cgcgcggcgg
    20581 cccgctcgcg cgccctccca ggcgcaaaat ccccccgcgt cggccgtggg gcgaccttcc
    20641 caggcgccgg agccggccaa gatcgcggat ccggccggcc aggccccggc gaccggcggg
    20701 acgggtggcc aaagccttcc cggcgccgaa ccccaggcgc ccgccccggc tccccccgca
    20761 agcccctcgc cggcggagaa ggccaagccc gcgatcccac cctccatagc ccagccccgg
    20821 gccttcggcc tcgcctccgt cgccggaccc gtcttccccg aggatttcgc catcggtccc
    20881 ctccaggacc tcaggccggc caggctggac gagaaggcgg cggccgaggc cgcgaccgcc
    20941 ttcctgggcg gcctcctcga ggggaaggtc gcggcggccg tcgtcctgcc ggagaggctc
    21001 gccctccttg aagccttcct ggccccgctt ttctcaggcg aaaggcctgt atcctaccgc
    21061 ctgggcgcga tagacttctc cgagggcccg ggagcccggg acggcgcttc ggccgtcgcc
    21121 ggcgtcgccc tcttcggggc ggcgggcgcg gtcggccggc gggcggccga gatcggcttg
    21181 cggcgaagcg gcgcggcctg gtacgtggag agcttccagg cggccgcgcc cggcggggac
    21241 gggaaggccg gagcggcgag cgtggcctac gagcccaggc tgcggcgccc ctaagggcgc
    21301 cgcccttcgg gacgccgccc ctaggcggcg aagaaaggaa gaggggagcg aagcatggcg
    21361 acgagcctga agcggatcga gaaggaattc atcctcggcg cggtgcgcga cgacggggtc
    21421 cgcgtcctcc tcatcgccgg caacggcgaa tggccggccg cgatcaccgc ggtcgacaag
    21481 gaccacctcg tcctctcgca ctcgatgccc cagcggctcc tccgccgcgg cgagacctac
    21541 gagttccgct tcgtctaccg ggagcaggcc atggccttcc gcgccaagct cctggaggcc
    21601 aaggaggc
//
LOCUS       DAOOSJ010000013        20673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1793_length_20673_cov_6.570957, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000013 DAOOSJ010000000
VERSION     DAOOSJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 20673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20673
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_20673_cov_6.570957"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..368)
                     /gene="csrA"
                     /locus_tag="PLG14_01515"
     CDS             complement(135..368)
                     /gene="csrA"
                     /locus_tag="PLG14_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOX47834.1"
                     /translation="MLILSRRVNEKIVIGDDVVVSIIEVRGDQVKIGIEAPRSVKVFR
                     QEVFNAIQEENRRAAAAASTAELPSIDLSGPGR"
     gene            complement(373..813)
                     /locus_tag="PLG14_01520"
     CDS             complement(373..813)
                     /locus_tag="PLG14_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOX47835.1"
                     /translation="MRFSTKAYGEIEIDERQRLRFPGGLLGFEKFTEYALLDARQKPF
                     FYLQSLEVPELAFILIDPFLFRPDYALDVGDESLAEIGLEGPEDAVVFAIVTAPGDGT
                     PITANLMGPIIVSKKKRLGMQAILADPRWRVKHDVMAELAATRK"
     gene            complement(838..2112)
                     /locus_tag="PLG14_01525"
     CDS             complement(838..2112)
                     /locus_tag="PLG14_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOX47836.1"
                     /translation="MQRVSTDMMNGDMQFWLRRTERDMASMETKMARQERIENLRDDP
                     LGAARAVRYDSVVGRLERFEKNAASAQDAYKVAEGYMRQAGEISQRLREIAVQGANGV
                     YTKEDQKYMGAEVDALLSELVSIANARGPDGSFLFAGEKARTEPFRAVKGASPLSGGE
                     VIQAVQYLGGIGSASAEVTEGSTIPLGQPGADIFWAERQQALSGYDARDWRAREDSAV
                     ILDGKRIEIKAGDTVHAVAAKINDSGAAVKATIDPRRFSISLEATLPHQIRAEDAKAA
                     GPAAGGAARPGVLEELGLVAGSGSPPDNWAPSARVSGGSLFDVAMRLRDSLNRGDVLE
                     TGGQALAGIDAGLDNLNRRAAELGARCERLETVQNRLNREIPDTTKLLAVEKDLDLTT
                     AITQYRMLEYAHKASLGFAGRLFPQTLLDFLR"
     gene            complement(2116..3990)
                     /gene="flgK"
                     /locus_tag="PLG14_01530"
     CDS             complement(2116..3990)
                     /gene="flgK"
                     /locus_tag="PLG14_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOX47837.1"
                     /translation="MQSTFLGIEIGKRGMNAHNQGLTTIGHNLTNASTEGYSRQRVEL
                     GTTEPLYAPSLNREETPGQIGQGVSIARIERVRDEILDGRIVAESGLEGYWSTRDKYV
                     LQLEQIHNEPSDSSVRTLMDKFWDSWQELSLRPADVPAREAVLSRGEALAEGIRDRYR
                     RLSELRTVIDGDVRGQVDQVNDLSRRIAELNGEIVRVKAMGDNPNDLMDRRDLLVERL
                     GTVIGVSVDRRDPDEIMVHADGIVLVQGKVSRSLAMEGADEDGYASPIWADTGLPFAA
                     KGGTLGASLELRDVDLRAEAQTLDTMALSFADLVNETHREGYGANGKTGLDFFKEWPF
                     INNLAGNYDRNGDGAYDSSYVYRITGGNALQAQEQVGLSGSIRLSKSGGEVEVPYYPT
                     DTVADLVSRINHSGAEVTARLTRDGRLQLRATPAASRQDPNFVIRHVEDSGQFLAGYA
                     GVLRGSGPEGAYDWARPDAVLSLRGGNLDYSVAPLAHPAGWLEVAPELKGDPGSIAAG
                     FGDNGRPAEAGDGRAALAIAALRTQPVMVGSERTFDDHFAEAVARVGLSGERAERGSE
                     SHARIGKELRDLRQSLSGVNLDEELANMIKFQHGYAAAAKFISHVDQMLDTIINRMGV
                     "
     gene            complement(4013..4513)
                     /gene="flgN"
                     /locus_tag="PLG14_01535"
     CDS             complement(4013..4513)
                     /gene="flgN"
                     /locus_tag="PLG14_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOX47838.1"
                     /translation="MMLETVAALAAALEAEIATFDAFVEGQKAFSAAIQSRDWLALER
                     SSKLLESLSAHLGAVEGERAEAEASLRRELGLSGRDEGFYRLVQLVPEPERGALADLF
                     RRLKIGAMRAKFENRAAGDYASANRELLGSVLEELFPEKKGRIYGRSGRAVQPDRGAI
                     LLDAAL"
     gene            4668..5537
                     /locus_tag="PLG14_01540"
     CDS             4668..5537
                     /locus_tag="PLG14_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47839.1"
                     /translation="MLRFLSEVLWLARGVRVYALVGESGTGKSFRAKLVAQKYGIEMI
                     IDDGLLIRGDSIIAGKSAKKEQLYMSAVKTALFHEKAHRDEVARSLQREKFRKILIIG
                     TSERMALKICDRLQIPHPVKVIKIEDIASQEEIAKAIRSRKVEGKHVIPVPALEIRRN
                     YPSIFYDSIRVFLKRSFGAAPSRPRLYEKSVVRPEYAKRGRVSISEAALSQMVIHCID
                     EFDDSVRVRKLMVRSDTQGYRLTVVLEVPYGTKLAQNARSLQEYIIENIERFTGILIE
                     EVNIVIDRFAARR"
     gene            5663..7408
                     /gene="ptsP"
                     /locus_tag="PLG14_01545"
     CDS             5663..7408
                     /gene="ptsP"
                     /locus_tag="PLG14_01545"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOX47840.1"
                     /translation="MREYKGIPASPGIAAAPAFVLREEEELSVPSYAIAPDAVEGEWN
                     RFLGAVEKAKAEVEELRDRARAAAGEEQAAIFEAHLLMLSDPDLFESIQGNLASSFRN
                     IERVIVEFERGLVEKLAGAEDPYLQARAADIHDVARRILSHLLFRERFTLADLESDVV
                     LIARDLLPSDMISLNRARVKAIATEAGGRTSHVAILARAFEIPAVLGLGEDLASVEAG
                     DMIVVDGSSGLVTSDPDAGVLSRAEASRSAAQALERELALLRDLPAATTDGRRLLLKA
                     NIEVPEEAVAALGHGAEGVGLFRSEFLFLQPGHVPGEDEQYRVYRRVIEIMDGRPVTI
                     RTLDIGGDKVLPELGALDEKNPLLGWRAIRFCLAKEEVFLTQLRAILRAAAHGDARIM
                     FPMISAPEELERARELVRQARAECRRRGHEVPDRIPVGIMIEVPSAALASDILARRAD
                     FFSIGTNDLIQYTMAVDRGNEKVAYLHDPFQPSVLRLVRQTIESAKGAGIPVGMCGEM
                     ASDPAAALLLLGLGLEEFSMSSSSLPAVKRLLRSVSAAEAEEASRAVMAMSSSAEVSA
                     YLAERAAAIAARRSL"
     gene            7445..9265
                     /gene="der"
                     /locus_tag="PLG14_01550"
     CDS             7445..9265
                     /gene="der"
                     /locus_tag="PLG14_01550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOX47841.1"
                     /translation="MKTTKDKKGAAAGKKGAEPAKKAPRRAPAKRKAPAGFRSTGLKK
                     APAKKAARKPRPEGEALREAAAAAEGKAVAEKAGRGRAGASARGAAKPAAAKPAAARK
                     GKSAAAPEEAARIEAVNPSSHAARAAAARAASRLAEGEAPAPAPAVGPAVLSASRRIR
                     YEGLPLVVIAGRPNVGKSTLYNRLLHKRRAITDPTPGVTRDPVDAVCELRDSGRRLRL
                     VDTGGFKLDREGLDSLVVEKSLSSLAKADLIVFLVDAMEITPEDEEFAALLRRWSPKV
                     LLVVNKADSPERDPLVWGHAKWGFEPVIFVSAEHGRNIDVLEAAMVARLDFSAVREVE
                     SARDEIRVAIMGKPNTGKSTLVNRLLKEEKSIVSDVAGTTRDVVEGRFEHKGRQVVVL
                     DTAGIRRKKKVSEAVEYYSVNRAIRTVDECDVVVLMIDAREGLTEQDKKITSFAVEKG
                     RGVIFALNKWDEMPDLKNSFEAARDKLRYFFGQLAYAPVLPLSAKEGTGVDKLLNMVV
                     TVHAELCREVETSKLNKAVEDWIEATPPPVGPRTRWKLRYAVQTSANPLKFVFFVSKP
                     DAVKEAYVSFLKNKIRDELGFGHVPVALELRASRTDRKKG"
     gene            9272..9637
                     /locus_tag="PLG14_01555"
     CDS             9272..9637
                     /locus_tag="PLG14_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX47842.1"
                     /translation="MASYGIGEVEDILGLPASTLRHWEKVLPALDPRKDAFGRRLYSD
                     ADLRLLFRLKHLAFGRGLGLKEAGEALLEELSSPGAEARARLAEIRGELIGLYFASRG
                     SGRELERRLAAAPPPAARP"
     gene            9739..11061
                     /locus_tag="PLG14_01560"
     CDS             9739..11061
                     /locus_tag="PLG14_01560"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOX47843.1"
                     /translation="MASASVKQLCDFVDASPTAFHAVENLQAALVARGAQKLSEGEAW
                     ALEPGRSYFVARSGSALVAFRPGLRPPAEEGFALAGAHTDSPALKARLEKPLSARGLE
                     RAPVEVYGGPILSTWLDRPLSLAGRVALRAQGGGLETRLLNFARPVGLIPNLAIHLNR
                     EVNKGFEYNAQAHLPVLLSSDPLAAAGASGGAATQAALLRLVAAELGVEAGAILGADL
                     YFVESQRVALIGADGGLVNGYRLDDLTGCHAVLEGFLAASPAPHGQVACFLDNEEVGS
                     RSVQGADSSFLRDILGRISALQGAGSEGFYRALSRSFSVSVDVAQAFHPSYAEKYDEA
                     FAPLLNGGPAVKVNANMKYATDAEAEARFRLLCEAKGVPCQKFMSRADTTPGSTIGPL
                     SSSLTGIRTVDVGAPLLSMHSIRETVGARDHEAMAAVLGLHFATPPSA"
     gene            complement(11216..11911)
                     /locus_tag="PLG14_01565"
     CDS             complement(11216..11911)
                     /locus_tag="PLG14_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47844.1"
                     /translation="MKRILLFALLAALLVAFLPAQGLADNEFYKKSLELKALADASFE
                     EGDYDAAADYAAQAQEQAALSDRYVEKMLAKASADKSIQAAKDRLAWADSVQAQGNFP
                     SEYALASAELEASYAAYGAEDYAAARERADAALVALAGVTEGLPWPAVYVVRLIPERR
                     DCLWRIAEYPFIYNNPLKWPVIYEANKKTFRDPTNPNLIFPGQKLSIPSIRGETRKGT
                     YDPSKKYEPFPKK"
     gene            complement(11924..13087)
                     /locus_tag="PLG14_01570"
     CDS             complement(11924..13087)
                     /locus_tag="PLG14_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47845.1"
                     /translation="MIRSKNPNSLFLCLVLAALAATGLTSCATKPKAPPAPETATTTE
                     PAATTPKTVETAPAVPQEELDKLLAEAAAGKKRAFDLKLFEILPEDYKAADAAFAAGK
                     TAYDAKDGPAAKEGLSKAVSLFNDLNARGIVELSAVKRKNADDMRATALKAGAEEKAA
                     ERFAPADASYAEAARLAEAGEHEKAVAAFERARALYELAYKRSLASGLREAIEEKGYA
                     AWDSGNHRLAENKYAEEERLFSAEGGAAELAAGIDSLDEAVLRYNLVVQKGRQGVANG
                     KKETTDEAKARSEGIKADVAVKEGFAEALALYEEGQAKFAAGDYEEAAEAFEKAGLRF
                     EEVYALAAEKRQRAVEAMSAAEAAAEESRRKAEAADQSGGAGWDATSGATAEE"
     gene            13288..14067
                     /locus_tag="PLG14_01575"
     CDS             13288..14067
                     /locus_tag="PLG14_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOX47846.1"
                     /translation="MQYEYFVLDIEDGIALLKINRPESLNALSSRLLSELEGILDELG
                     RDDRVAALVVTGEGRAFVAGADIEEMRGLGAEEGRRFGALGSRVFRKLELLRQPVIAA
                     VNGFALGGGLELAMACDIRLAGDKAKFGQPEVGLGITPGFSGTQRLPRIVGAAKAKEL
                     IFTGEMIKAEEALRIGLVSRVLPAEELLPAAMELARKIAKNAPLAVAYSKAAINRGLE
                     EGLESGIALEESLFGLCFATADQKEGMTAFLEKRPAAFAGK"
     gene            14147..14992
                     /locus_tag="PLG14_01580"
     CDS             14147..14992
                     /locus_tag="PLG14_01580"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOX47847.1"
                     /translation="MKVMVLGAGTMGSGIAQVFALSGHSVVLRDIEQAFADKGLAGIA
                     KNLEKAVAKGNLAAAAKDEALARIVATADLGLARDVDLVVEAAVENMAIKRKVFAELD
                     GVCKSEAILATNTSSLSITEIAAATKRPDRVVGMHFFNPVPVMKLVEVIKGIATSEET
                     KKAVLELALSLGKTPVEVEEAPGFVVNRILIPMINEAIGILADGVASAADIDEAMKLG
                     ANHPIGPLALADLIGNDVNLAIMETLYEEFGDPKYRPHPLLRKMVRGGLLGRKSGKGF
                     FDYAK"
     gene            15026..15412
                     /locus_tag="PLG14_01585"
     CDS             15026..15412
                     /locus_tag="PLG14_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOX47848.1"
                     /translation="MEKSLIRLRMSSHDAHYGGNLVDGAKMLQLFGDVATELLILHDG
                     DEGLFAGYEKVEFLAPVFAGDYIEASGEIVSVGNSSRKMTFEARKVIAPRPDLNDSAA
                     DLLPEPVVVCRAVGTCVVPKAKQRKS"
     gene            15540..16679
                     /locus_tag="PLG14_01590"
     CDS             15540..16679
                     /locus_tag="PLG14_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOX47849.1"
                     /translation="MDFQADKEHRLLQEMYRDFAETEVKPLAKEVDERELFPVETVRK
                     MARYGFMGIPFPKEYGGSGGDNLAYIMAVEALSKVCGTTGVVLSAHSSLCAAPIYEFG
                     TEEQKRKYLVPLAKGEKLGAFGLTEPNAGTDASGQQTTAEPKEGFYLLNGSKLFITNA
                     GYADLYIVFAMTDRTKGTRGISAFIVEKGMKGFSIGKKEEKMGIRGSSTCELVFENVE
                     VPAANLLGKEGGGFGIAMKTLDGGRIGIAAQALGLAQGAIDETVAYVKERKQFGKALA
                     QFQNTQFQLADMQARTDAARLLVYRAAASKDAKLPYAVEAATAKLFASETAMEVTTKA
                     VQLHGGYGYTREYPVERMMRDAKITEIYEGTSEVQRMVISAAMLK"
     gene            16693..17472
                     /locus_tag="PLG14_01595"
     CDS             16693..17472
                     /locus_tag="PLG14_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX47850.1"
                     /translation="MNIVVCVKQVPDTTEVKIDPATGTLVREGIPSIINPDDKSGLEA
                     ALELKDQVGAKVTVLSMGPPQAELALREALAMGADEAILLSDRAFGGADTWATSTTLA
                     AALRKLDWDLVVCGRQAIDGDTAQVGPQIAEHLGIPHVSYVSELKVEGEAVVVKRAFE
                     DGYQRIRAKFPCLVTALKDMNKPRYMSVGGVFDAYRKTIPVWGLADLDVDPANIGLKG
                     SPTRVKSSYTKGTKSAGKVYEVDPKEGARIIVERLKEKFII"
     gene            17536..18549
                     /locus_tag="PLG14_01600"
     CDS             17536..18549
                     /locus_tag="PLG14_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX47851.1"
                     /translation="MNVSEYKGVFVFAERRDGAVQKVAYELLGMGRGIADALGEPLAA
                     VLLGSGLKDSEAAELIAFGADEVILVDHPDLGVYMTEPYTKAVVKAIAERKPGVVFFG
                     ATTIGRDLAPRVSARVKTGLTADCTALAVEDGTRNLLMTRPAFGGNIMATIVCPEHRP
                     QMSTVRPGVMRKLERDGARKGRVSRLAVEIEAKDRNVEILEVVSEKVARKNIEDSTVL
                     VSGGRGLGSPENFRKLDLVADELGGLVSASRAVVDLGWVGQDRQVGQTGKTVRPDLYI
                     ACGISGMIQHIAGMEDSGFIVAVNKNSGAPIFEYADLGIVTDVNRLVPALAEELKAAK
                     AER"
     gene            18740..19015
                     /locus_tag="PLG14_01605"
     CDS             18740..19015
                     /locus_tag="PLG14_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HOX47852.1"
                     /translation="MARYDFANEGTCSESVSFSLDGGRLASVRFSGGCPGNLAGIARL
                     VEGLEAEEAARRLEGIRCGSKSTSCPDQLAKAIRRALGAATTQGSAP"
     gene            19012..20139
                     /locus_tag="PLG14_01610"
     CDS             19012..20139
                     /locus_tag="PLG14_01610"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOX47853.1"
                     /translation="MNSQARFRLGPIAAGVLLLAVLLVAPTACATSGYDAEAASLSSY
                     AEKDGGLVLRLARDGSLVWGAWEDLSAKRFGLVEGEARPDGGLSLKLFGESASAPPAT
                     LEGRYAAGEGRVEGKLAGGDASRDVVLPRSAAPAKLALSRLHAEERGSLAAEAEPTRF
                     DYTSIEPAAPKEFLDWYRKTFQGGMDSGAAMAREKDAFFEDFRSGSQALLEAAGQEAV
                     HPWFYDGRQFLAYSNPSVLVLGLRRATFAGGAHGMHGVRYAIIDPEEPRILGPADLFV
                     PGCDEALSGLLGAKAREAFGLEPGSSLSQGGFLVEAIPPTNNMFVYSGGIGFHYNVYE
                     VAPYAAGDIWLCLPFEELGGVLLPGAAERLGFPAGKEGAKP"
     gene            20136..20576
                     /locus_tag="PLG14_01615"
     CDS             20136..20576
                     /locus_tag="PLG14_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOX47854.1"
                     /translation="MTESLEMYLETISLLHDRSRIARVTDIAAELGVSKPSVHTALHE
                     LERRGLIEHEHYGEVFLTQSGKAASAAIRERHGLLTAFLRDFLGVSPAVAEQDACRME
                     HILSEETMARIAERTRGAAAAGRGGSAPGAAQPGPAEPADAARP"
BASE COUNT         3091 a         7472 c         7045 g         3065 t
ORIGIN      
        1 gaaagcctca tcgaacctcc gcgccgatta tcgtatggag aaacactagc gcatgaattc
       61 gataaaagca aattgagtgg gaggaagagg cgcgggcggg aaaggcgcgc gaggcgcctt
      121 gaaggcttcg gggcctagcg ccccggcccg gagaggtcga tgctcggaag ctcggcggtg
      181 gaggcggcgg ccgcggcccg gcggttctcc tcctggatgg cgttgaagac ctcctggcgg
      241 aagaccttga cgctcctcgg ggcctcgatg ccgatcttca cctggtcgcc gcggacctcg
      301 atgatggaga cgaccacgtc gtcgccgatg acgatcttct cgttgacccg gcgggagagt
      361 atcagcatgg cttcacttcc tcgtcgccgc gagctcggcc atcacgtcgt gcttcacgcg
      421 ccagcgggga tccgcgagga tggcctgcat gccgagccgc ttcttcttgg agacgatgat
      481 ggggcccatg aggttcgcgg tgatgggagt gccgtcgccc ggggccgtga cgatggcgaa
      541 gacgacggcg tcctccggtc cctccaggcc gatctccgcg aggctctcgt cgccgacgtc
      601 cagggcgtag tcgggcctga agaggaaggg atcgatgagg atgaaggcga gctcgggtac
      661 ctccagcgac tgcaggtaga agaagggctt ttgccgggcg tcgaggaggg cgtactcggt
      721 gaacttctcg aagccgagga ggccgccggg gaagcgcagg cgctggcgct cgtcgatctc
      781 gatctctccg taagccttag tgctgaatct catggtgtgg ccccactatg ccgggcttca
      841 gcgcaggaag tcaaggagag tctgggggaa gagacgcccg gcgaagccga gcgaggcctt
      901 gtgcgcgtac tccagcatgc ggtactgcgt gatcgccgtc gtgaggtcca ggtccttctc
      961 gaccgcgagg agcttcgtcg tgtcggggat ctcgcggttc aggcggttct ggaccgtctc
     1021 cagccgctcg cacctcgcgc cgagctcggc ggcgcggcgg ttcaggttgt cgaggccggc
     1081 gtcgatgccc gcgagggcct ggccgccggt ctcgaggacg tcgccgcggt tcagggagtc
     1141 gcggaggcgc atcgccacgt cgaagaggga gccgcccgag acgcgggcgc tcggggccca
     1201 gttgtccggc ggcgagccgg agccggcgac gaggccgagc tcctccagga cgcccggcct
     1261 ggctgcgccg cccgcggcgg gtccggcggc cttcgcgtcc tccgcgcgga tctggtgggg
     1321 gagggtggcc tccagggaga tggagaagcg gcgggggtcg atggtcgcct tgaccgcggc
     1381 gccggagtcg ttgatcttcg ccgcgacggc gtggaccgtg tcgcccgcct tgatctcgat
     1441 ccgcttcccg tccaggatga ccgcggagtc ctccctggct cgccagtccc tcgcgtcgta
     1501 gcccgagagg gcctgctggc gctcggccca gaagatgtcg gcccccggct ggcccaaggg
     1561 gatggtcgag ccctccgtga cctcggctga ggcgctcccg atgccgccca ggtactgcac
     1621 cgcctggatc acctcgccgc ctgagagggg ggaggcgccc ttcaccgcgc ggaagggctc
     1681 ggtcctggcc ttctcccccg cgaagaggaa ggagccgtcg ggcccgcgcg cgttggcgat
     1741 ggagacgagc tcggagagga gggcgtcgac ctcggcgccc atgtacttct ggtcttcctt
     1801 ggtgtagacg ccgttcgcgc cctgcacggc tatctcgcgg aggcgctggc ttatctcgcc
     1861 ggcctgcctc atgtagccct cggcgacctt gtaggcgtcc tgggcgctcg cggcgttctt
     1921 ctcgaagcgc tccaggcgcc ccacgacgga gtcgtagcgc acggccctcg cggcgcccag
     1981 ggggtcgtcg cggaggttct cgatgcgctc ctggcgcgcc atcttcgtct ccatggaggc
     2041 catgtcgcgc tcggtcctgc ggagccagaa ctgcatgtcc ccgttcatca tgtccgtgct
     2101 gacgcgctgc ataggttaca ctcccatcct gttgatgatg gtgtcgagca tctggtcgac
     2161 gtgcgagatg aatttcgccg cggccgcgta gccgtgctgg aacttgatca tgttggcgag
     2221 ctcctcgtcc aggttgaccc cggagaggga ctgcctgagg tcgcggagct ccttgccgat
     2281 gcgcgcgtgg gactcgctgc cgcgctccgc gcgctcgccc gagagcccga cgcgggccac
     2341 ggcctccgcg aagtggtcgt cgaaggtccg ctcgctcccc accatcacgg gctgggtccg
     2401 cagggccgcg atcgcgaggg ccgcgcggcc gtcaccggcc tccgccggcc tgccgttgtc
     2461 gccgaagccc gcggctatgg agcccgggtc gcccttgagc tcgggcgcga cctcgagcca
     2521 gcccgcgggg tgggcgaggg gggccacgga gtagtccagg ttgccgccgc gcagggagag
     2581 caccgcgtcg ggcctggccc agtcgtaggc gccctcgggg cccgacccgc gcaggactcc
     2641 cgcgtagcca gcgaggaact ggcccgagtc ctccacgtgg cggatgacga agttcgggtc
     2701 ctggcgcgag gccgccggcg tcgcgcgcag ctggagcctg ccgtcgcggg tgagcctcgc
     2761 ggtcacctcc gcgcccgagt ggttgatcct ggagaccagg tcggccacgg tgtcggtcgg
     2821 gtagtagggc acctctacct cgccgccgga cttcgagagc ctgatcgagc ccgagaggcc
     2881 gacctgctcc tgggcctgga gggcgttgcc gcccgtgatg cggtagacgt agctcgagtc
     2941 gtaggcgccg tccccgttcc ggtcgtagtt gccggcgagg ttgttgatga agggccactc
     3001 cttgaagaag tcgaggccgg tcttgccgtt ggcgccgtag ccctcgcggt gcgtctcgtt
     3061 cacgaggtcg gcgaaggaga gggccatcgt gtcgagggtc tgggcctcgg cgcgcaggtc
     3121 cacgtcgcgg agctcgaggc tcgcgcccag agtgccgccc ttcgcggcga aggggaggcc
     3181 cgtgtcggcc cagatcgggc tcgcgtagcc gtcctcgtcg gccccctcca tcgcgaggga
     3241 gcgggagacc ttgccctgga cgaggacgat gccgtccgcg tggaccatga tctcgtcggg
     3301 gtcgcggcgg tccacggaga cgccgatgac ggttcccagc cgctccacga ggaggtcgcg
     3361 gcggtccatg aggtcgttgg ggttgtcgcc catcgccttc acgcggacga tctcgccgtt
     3421 gagctcggcg attcggcggg agaggtcgtt gacctggtcc acctggccgc ggacgtcgcc
     3481 gtcgatgacc gtcctgagct cggagaggcg gcggtagcgg tccctgatgc cctcggccag
     3541 ggcctcgccc cgggagagca cggcctcgcg ggcggggacg tcggcggggc gcagggagag
     3601 ctcctgccag gagtcccaga acttgtccat gagggtgcgc accgaggagt ccgagggctc
     3661 gttgtggatc tgctcgagct ggagcacgta cttgtcgcgg gtcgaccagt agccctccag
     3721 gcccgactcg gcgacgatgc ggccgtccag gatctcgtcg cggacgcgct cgatgcgcgc
     3781 gatggacacg ccctggccga tctggcccgg cgtctcctcg cggttcaggg agggggcgta
     3841 gagcggctcc gtcgtcccga gctcgacgcg ctgccgggag tagccctccg tcgaggcgtt
     3901 ggtgaggttg tggcctatcg tcgtgaggcc ctggttgtgc gcgttcatgc cgcgcttgcc
     3961 gatctctatg cccaggaagg tggactgcat ggcttttccg ctcctatggc ccctagaggg
     4021 ccgcgtcgag gagaatggcc ccgcggtcgg gctggactgc cctgccggag cggccgtaga
     4081 tgcggccctt cttctcgggg aagagctcct ccagcacgga gccgaggagc tcgcggttgg
     4141 cggaggcgta gtcgccggcc gccctgttct cgaatttggc gcgcatggcc cctatcttga
     4201 gccggcggaa gaggtcggcg agggcgcccc gctcgggctc gggcacgagc tggacgaggc
     4261 ggtagaagcc ctcgtcccgg ccggagaggc cgagctcgcg gcgcagggag gcctcggcct
     4321 cggcccgctc cccctcgacg gcgcccaggt gggcggagag gctttcgagg agcttggagc
     4381 tccgctccag ggcgagccag tcgcgggact ggatggcggc ggagaaggct ttctggccct
     4441 cgacgaaggc gtcgaaggtg gcgatctcgg cctcgagggc ggcggcgagg gcggcgactg
     4501 tctcaagcat catactgcgc acactccttc gctccatcat cggcattccc cggccccggt
     4561 ttagccaatt cggccccccg gggccccggc gccccgcgcg cccccgcccc cccatggcgc
     4621 cggagacgat tgcgccgggg ggccggctcc ggctatagtc gagcgacatg ctgcggtttt
     4681 tgagcgaggt cctgtggctc gcgaggggcg tccgcgtcta cgccctcgtg ggcgagagcg
     4741 gcacgggcaa gagcttccgc gccaagctcg tggcgcagaa gtacgggatc gagatgatca
     4801 tcgacgacgg actcctcatc cgcggcgact ccatcatcgc gggcaagtcc gcgaagaagg
     4861 agcagctcta catgtccgcg gtgaagaccg ccctcttcca cgagaaggcc caccgcgacg
     4921 aggtcgcccg ctccctgcag cgcgagaagt tccggaagat cctcatcatc gggacctccg
     4981 agcgcatggc cctcaagatc tgcgaccgcc tccagatccc ccacccggtc aaggtcatca
     5041 agatcgagga catcgcgagc caggaggaga tcgccaaggc gatccgctcc cgcaaggtcg
     5101 aggggaagca cgtcatcccc gttccggccc tggagatccg gcgcaactac ccctcgatct
     5161 tctacgactc gatccgcgtc ttcctgaagc ggagcttcgg cgcggccccc tcccgccccc
     5221 ggctctacga gaagtccgtc gtcaggcccg agtacgccaa gcggggccgc gtctccatct
     5281 cggaggcagc cctctcccag atggtgatcc actgcatcga cgaattcgac gattccgtca
     5341 gggtgcggaa gctcatggtc cgctcggaca cccagggcta ccgcctgacc gtggtcctgg
     5401 aggtgcccta cggcaccaag ctcgcccaga acgcccgctc cctccaggag tacatcatcg
     5461 agaacatcga gcgcttcacg ggcatcctca tcgaggaagt gaacatcgtc atcgaccgct
     5521 tcgccgcccg ccgctagccg cccgcgcccc cggcgccccc ggaactccgc gccgcgcggc
     5581 ggcggcggcc ccgtcccgcg gggccagccc cgctctgcgg ggctttgaca gctatccccc
     5641 tatctggtaa agtcgtaacg gcatgaggga atacaaggga ataccggcgt cgccgggcat
     5701 cgcggccgcc ccggccttcg tcctccgcga ggaggaggag ctctcggtgc cgagctacgc
     5761 catcgccccc gacgcggtcg agggggagtg gaaccgcttc ctcggcgccg tggagaaggc
     5821 caaggccgag gtcgaggagc tccgcgaccg ggcccgcgcc gccgccggcg aggagcaggc
     5881 cgcgatcttc gaggcccatc tcctcatgct ctccgatccc gacctcttcg agagcatcca
     5941 gggcaacctg gcctcgagct tccgcaacat cgagcgcgtc atcgtcgagt tcgagcgcgg
     6001 cctggtggag aagctggccg gcgcggagga cccctacctc caggcccggg ccgcggacat
     6061 ccacgacgtc gcgcggcgca tcctctccca cctcctcttc cgggagcgct tcaccctcgc
     6121 cgacctcgag tccgacgtcg tcctaatcgc ccgcgacctc ctgccctccg acatgatctc
     6181 cctgaaccgc gcccgcgtga aggcgatcgc gacggaggcg ggcggccgca cgagccacgt
     6241 cgccatcctg gcccgagcct tcgagatccc ggccgtcctc ggcctgggcg aggacctggc
     6301 ctccgtcgag gcgggcgaca tgatcgtcgt ggacggctcc tccggcctcg tgacctccga
     6361 ccccgacgcc ggggtcctct cccgcgcgga ggcctcgagg agcgcggccc aggccctgga
     6421 gcgcgagctc gccctactcc gcgacctccc cgccgcgacg accgacggcc gccgcctcct
     6481 cctcaaggcg aacatcgagg tgcccgagga ggccgtggcc gccctgggcc acggcgccga
     6541 gggcgtcggc ctcttccgct cggagttcct cttcctccag ccgggccacg ttcccgggga
     6601 ggacgagcag taccgcgtct accgcagggt catcgagatc atggacggcc ggcccgtcac
     6661 gatccgcacc ctggacatcg gcggcgacaa ggtcctcccg gagctcgggg ccctggacga
     6721 gaagaacccc ctcctgggct ggcgcgccat ccgcttctgc ctcgccaagg aggaggtctt
     6781 cctcacccag ctccgcgcca tcctccgcgc ggccgcccac ggggacgcgc gcatcatgtt
     6841 ccccatgatc tccgcgcccg aggagctgga gcgcgcgagg gagctcgtgc gccaggcccg
     6901 cgccgagtgc cgccgccgcg gccacgaggt gcccgaccgc atccccgtcg gcatcatgat
     6961 cgaggtgccc tccgcggccc tcgcgagcga catcctggcc cgccgcgccg acttcttctc
     7021 catcgggacg aacgacctca tccagtacac gatggccgtc gaccgcggca acgagaaggt
     7081 cgcctacctc cacgacccct tccagccctc ggtgctccgc ctggtcaggc agaccataga
     7141 gagcgccaag ggcgccggca tccccgtcgg catgtgcggc gagatggcct ccgatcccgc
     7201 cgccgccctc ctcctcctcg gcctcggcct cgaggagttc tccatgtcga gctcgtccct
     7261 cccggcggtg aagcgcctcc tccgctcggt ctccgcggcc gaggccgagg aggcttcccg
     7321 cgccgtcatg gcgatgtcct cctccgcgga ggtctccgcc tatctcgccg agcgcgccgc
     7381 cgcaatcgcg gcgcgccgca gcctttaagc cgcgggccgc tcaggcgccc gcaccacgga
     7441 gcacatgaaa accacgaagg acaagaaggg cgccgccgcc ggaaagaagg gcgcggaacc
     7501 cgcgaagaag gcgccgcgcc gcgcccccgc caagcgcaag gcccccgcgg gcttccgctc
     7561 gaccggcctc aagaaggccc ccgccaagaa ggccgcgaga aagcccaggc ccgagggcga
     7621 agccctccgc gaggccgccg ccgccgccga aggcaaggcc gtcgccgaga aggcgggcag
     7681 gggcagggcc ggcgcctcag cccgcggcgc cgccaagccc gccgccgcca agccggcggc
     7741 ggcgcgcaag ggcaagtccg cggccgcgcc ggaggaggcc gcgaggatcg aggcggtcaa
     7801 tccctcctcc cacgcggccc gcgccgcggc ggcccgcgcc gcctcccgcc tcgccgaggg
     7861 cgaggctccg gccccggctc cggccgtcgg cccggccgtc ctctccgcct cgcgccgcat
     7921 ccgctacgag ggcctccccc tcgtcgtcat cgcgggcagg cccaacgtcg ggaagtcgac
     7981 gctctacaac cgccttctcc acaagcgccg ggccatcacg gacccgacgc ccggcgtcac
     8041 gcgcgacccc gtggacgcgg tctgcgagct ccgcgactcg ggccgccgcc tccgcctcgt
     8101 ggacacgggc ggcttcaagc tcgaccgcga gggcctcgac tccctcgtcg tggagaagag
     8161 cctttcctcc ctcgccaagg ccgacctcat cgtcttcctg gtcgacgcga tggagatcac
     8221 gcccgaggac gaggagttcg ccgccctcct caggcgctgg agccccaagg tcctcctcgt
     8281 cgtcaacaag gccgacagcc ccgagcgcga ccccctcgtg tggggccacg cgaagtgggg
     8341 cttcgagccc gtcatcttcg tctccgcgga gcacggacgc aacatcgacg tcctggaggc
     8401 cgcgatggtc gcgcgcctgg acttcagcgc cgtccgcgag gtggagtcgg cccgcgacga
     8461 gatccgcgtc gccatcatgg gcaagccgaa caccggcaag tccaccctcg tgaaccgcct
     8521 cctcaaggag gagaagtcca tcgtgagcga cgtcgccggc acgacccgcg acgtcgtgga
     8581 gggccgcttc gagcacaagg gcagacaggt cgtcgtcctg gacaccgccg gcatcaggcg
     8641 gaagaagaaa gtctccgagg cggtggagta ctactccgtg aaccgcgcca tcaggacggt
     8701 ggacgagtgc gacgtcgtcg tcctcatgat cgacgcccgc gagggcctca ccgagcagga
     8761 caagaagatc acctccttcg ccgtggagaa ggggaggggc gtgatcttcg ccctcaacaa
     8821 gtgggacgag atgcccgacc tcaagaacag cttcgaggcg gcccgcgaca agctccgcta
     8881 cttcttcggc cagctcgcct acgcccccgt ccttcccctc tccgcgaagg agggcacggg
     8941 cgtcgacaag ctcctcaaca tggtcgtcac cgtccacgcg gagctctgcc gcgaggtgga
     9001 gacctcgaag ctcaacaagg ctgtcgagga ctggatcgag gcgacgccgc cccccgtcgg
     9061 cccccggacc cgctggaagc tccgctacgc cgtccagacg agcgcgaacc ccctcaagtt
     9121 cgtcttcttc gtctccaagc ccgacgcggt gaaggaggcc tatgtctcct tcctcaagaa
     9181 caagatccgc gacgagctcg gcttcggcca cgtgcccgtc gccctcgagc tcagggcctc
     9241 gcggacggac cgcaagaagg gctagggccc cgtggcctcc tacgggatcg gggaggtcga
     9301 ggacatcctc ggccttccgg cctcgaccct gaggcactgg gagaaggtcc tcccggccct
     9361 cgatccccgc aaggacgcct tcggccgccg cctctactcg gacgccgacc tccgcctcct
     9421 cttcaggctc aagcacctcg ccttcggccg cggcctgggc ctgaaggagg ccggcgaggc
     9481 cctccttgag gagctctcct cgcccggcgc cgaggccagg gcccgcctcg cggagatccg
     9541 cggcgagctc atcggcctct acttcgcgag ccgcggaagc ggccgcgagc tcgagcgccg
     9601 cctcgccgcc gccccgcctc cggcggcgcg gccctaggcc cgcccgttcc gccgccgcgg
     9661 cccgcccggc gccgcgccgc gcgccttccc gcgcctccgc cctccgggct atactggccg
     9721 gcggaggact tacaccctat ggccagcgca tccgtcaagc agctctgcga tttcgtcgac
     9781 gcctctccca cggccttcca cgcggtggag aacctgcaag ccgccctcgt cgcgcggggc
     9841 gcgcaaaagc tgtcggaggg ggaggcctgg gccctcgagc ccgggaggag ctacttcgtc
     9901 gcgcgctcgg gctcggccct cgtcgccttc aggccgggcc tgcggccccc cgcggaggag
     9961 ggcttcgccc tcgcgggcgc ccacaccgac agcccggccc tgaaggcccg cctcgagaag
    10021 ccactctccg cgcggggcct cgagcgcgcc cccgtggagg tctacggcgg ccccatcctc
    10081 tcgacctggc tcgaccggcc cctctccctc gcgggcaggg tcgccctgcg cgcccaaggc
    10141 ggcggcctgg agacgaggct cctcaacttc gcgcgtcccg tcggactcat cccgaacctg
    10201 gccatccacc tgaaccgcga ggtcaacaag ggcttcgagt acaacgccca ggcccatctc
    10261 cccgtcctcc tctcctccga tcccctcgcc gcggcgggcg cctccggcgg agcggccacg
    10321 caggcggccc tcctccgcct cgtcgccgcc gagctcgggg ttgaggcggg ggccatcctc
    10381 ggcgccgacc tctacttcgt cgagtcccag cgcgtcgccc tgatcggcgc cgacggcggc
    10441 ctcgtcaacg gctaccgcct ggacgacctc acgggctgcc acgccgtcct cgagggcttc
    10501 ctcgccgcgt ccccggcccc ccacggccag gtggcctgct tcctcgacaa cgaggaggtg
    10561 ggttcccgga gcgtccaggg cgccgactcc tccttcctcc gcgacatcct gggccggatc
    10621 tccgccctcc agggcgcggg cagcgagggc ttctaccgcg ccctctcccg ctccttctcc
    10681 gtctccgtcg acgttgccca ggccttccac ccatcctacg cggagaagta cgacgaggcc
    10741 ttcgcccccc tcctcaacgg cgggcccgcg gtgaaggtca acgcgaacat gaagtacgcg
    10801 accgacgcgg aggcggaggc ccgcttccgc ctcctctgcg aggcgaaggg cgtcccctgc
    10861 cagaagttca tgagccgcgc cgacacgacg cccggctcca cgatcggccc cctctcctcc
    10921 tccctcacgg gcatccgcac cgtggacgtg ggcgcgcccc tcctgtccat gcactccatc
    10981 agggagaccg tcggcgcccg cgaccacgag gccatggcgg ccgtcctggg cctccacttc
    11041 gcgacgccgc ccagcgccta ggcgggtggg cagcgccgcc ggggcctcgc cggcactggc
    11101 cgcgcgcccg cagagaccgc ttgccgcggc gcggcgacga cgagagagga acgagagcat
    11161 aaaagaaggg gctgcccgcg cgggcagccc cagtctttgc atagcgggaa ggtcgttact
    11221 tcttcgggaa gggctcgtac ttcttcgagg gatcgtaggt ccccttcctc gtctcgccgc
    11281 ggatgctcgg aatcgagagc ttctggccgg ggaagatgag gttcgggttc gtcgggtccc
    11341 tgaaggtctt cttgttcgcc tcgtagatca cgggccactt caggggattg ttgtagatga
    11401 aggggtactc ggcgatccgc cagaggcagt cgcggcgctc ggggatcagg cggacgacgt
    11461 agacggccgg ccagggaagg ccctccgtca cgccggccag cgcgaccagg gcggcgtcgg
    11521 cgcgctcgcg ggcggccgcg tagtcctccg ccccgtaggc cgcgtaggag gcctcaagct
    11581 cggccgaggc gagggcgtac tccgacggga agttgccctg ggcctggacc gagtcggccc
    11641 aggccaggcg gtccttcgcc gcctggatgg acttgtcggc cgaggccttg gcgagcatct
    11701 tctcgacgta gcggtcggag agagcggcct gctcctgggc ctgggccgcg tagtcggcag
    11761 cggcgtcgta gtcgccctcc tcgaaggagg cgtcggccag ggccttgagc tccaggctct
    11821 tcttgtagaa ctcgttgtcc gcgaggccct gggcgggcag gaaggcgacg aggagggcgg
    11881 cgagcagggc gaaaagtagt atgcgcttca tggctttccc tccttactct tccgccgtgg
    11941 cgcccgaggt ggcgtcccat ccggcgccgc ccgactggtc ggcggcctcg gccttgcggc
    12001 ggctctcctc ggcggcggcc tcggcggcgc tcatcgcctc cacggccctc tgccgcttct
    12061 cggcggccag ggcgtagacc tcctcgaagc ggaggccggc cttctcgaag gcctccgcgg
    12121 cctcctcgta gtcgccggcg gcgaacttgg cctgcccctc ctcgtagagg gccagggcct
    12181 cggcgaagcc ctccttgacg gcgacgtcgg ccttgatgcc ctcggagcgg gccttggcct
    12241 cgtccgtggt ctccttcttg ccgttggcca cgccctggcg gcccttctgg acgaccaggt
    12301 tgtagcgcag gacggcctcg tccagggagt cgatgccggc ggcgagctcg gcggccccgc
    12361 cctcggcgga gaagaggcgc tcttcctcgg cgtacttgtt ctcggccagg cggtggttgc
    12421 ccgaatccca ggcggcgtag cccttctcct cgatggcctc gcggaggccg gaggcgaggc
    12481 tgcgcttgta ggcgagctcg tagagggcgc gggcgcgctc gaaggccgcg acggccttct
    12541 cgtgctcgcc cgcctcggcg agcctggcgg cctcggcgta ggaggcatcg gcgggggcga
    12601 agcgctcggc ggccttctcc tcggcgcccg ccttcagggc cgtcgcgcgc atgtcgtcgg
    12661 cgttcttccg cttcacggcg gagagctcga cgatgccgcg ggcgttgagg tcattgaaga
    12721 ggctcacggc cttggagagg ccttccttgg cggcggggcc gtccttggcg tcgtaggccg
    12781 tcttgcccgc cgcgaaggcg gcgtcggcgg ccttgtagtc ttcggggagt atctcgaaaa
    12841 gcttcaggtc gaaggcccgc ttcttgccgg cggcggcctc ggcgaggagc ttgtcgagct
    12901 cctcctgcgg caccgcgggg gcggtctcga cggtcttggg cgtcgtggcg gcgggttccg
    12961 tcgtcgtggc ggtctcgggc gcgggcgggg ccttgggctt cgtcgcgcag gaggtcaggc
    13021 cggtcgccgc cagggcggcc aggacgaggc agaggaagag gctgttgggg tttttacttc
    13081 ggatcattgg ctcctcctag ggtgaggttg ttacattgaa aagaatagca taaaagccac
    13141 ctgaggcaaa cctttgggcg agtattattt cccctatcgg taggcttctt attgtagaaa
    13201 cttgaatcga taaaaaatgc ttgacaagac agaaggccgg agctatgcta ttctcaccct
    13261 ctagcagagc cacgcaagcg aggtcccatg cagtacgagt acttcgttct cgacatcgaa
    13321 gacgggattg ccctcctcaa gatcaacagg cccgagagcc tgaacgcgct cagctccagg
    13381 ctcctttccg agctggaggg catcctggac gagctcgggc gcgacgaccg ggtcgccgcc
    13441 ctcgtcgtca cgggagaggg ccgggccttc gtcgccggcg ccgacatcga ggagatgcgc
    13501 ggccttggcg cggaggaggg gaggcgcttc ggcgccctgg gctccagggt cttcaggaag
    13561 ctcgagctcc tccgccagcc cgtcatcgcc gcggtgaacg gcttcgccct gggcggaggc
    13621 ctggagctcg cgatggcctg cgacatcagg ctcgcggggg acaaggccaa gttcggccag
    13681 cccgaggtgg gcctgggcat cacgcccggc ttctccggca cccagcgcct gcccaggatc
    13741 gtcggcgccg ccaaggccaa ggagctcatc ttcacgggag agatgatcaa ggccgaggag
    13801 gccctgcgca taggcctcgt ctcccgggtc ctccccgcgg aggagctcct ccccgccgcg
    13861 atggagctcg ccaggaagat cgccaagaac gcgccgctcg ccgtcgccta ctccaaggcc
    13921 gcgatcaacc gcggcctcga ggagggcctg gagagcggca tcgccctgga ggagagcctt
    13981 ttcggcctct gcttcgccac ggccgaccag aaggagggca tgacggcctt ccttgagaag
    14041 cggcccgccg ccttcgccgg gaaataggcc aaggccccat ggcggagcgg tccgcgaagg
    14101 cccgcccgcc ggttcgaagg ccggtcgccg gccaaaagga gcttgcatga aagtcatggt
    14161 attgggcgcc gggaccatgg gctcgggcat cgcgcaggtc ttcgcccttt cgggccactc
    14221 ggtcgtcctg cgggacatcg agcaggcctt cgccgacaag gggcttgcgg gcatagcgaa
    14281 aaacctcgag aaggcggtgg ccaaggggaa cctggccgcg gccgccaagg acgaggcctt
    14341 ggccaggatc gtcgccaccg ccgacctggg cctggcccgg gacgtcgacc tcgtggtgga
    14401 ggcggccgtg gagaacatgg cgatcaagcg gaaggtcttc gcggagctcg acggggtctg
    14461 caagagcgaa gccatcctcg cgaccaacac ctcctccctg tcgatcacgg agatcgcggc
    14521 cgccaccaag cgccccgacc gcgtcgtcgg catgcacttc ttcaaccccg tgccggtgat
    14581 gaagctcgtg gaggtgatca agggcatcgc cacctccgag gagaccaaga aggccgtcct
    14641 ggagctcgcc ctctccctgg ggaagacccc cgtggaggtc gaggaggccc ccggcttcgt
    14701 cgtgaaccgc atcctcatcc cgatgatcaa cgaggccatc ggcatcctgg ccgacggggt
    14761 cgcgagcgcc gccgacatcg acgaggcgat gaagctcggc gccaaccacc ccatcggccc
    14821 cctggccctc gccgacctca tcggcaacga cgtcaatctc gcgatcatgg agaccctcta
    14881 cgaggaattc ggcgacccga agtaccggcc ccaccccctg ctcaggaaaa tggtgcgcgg
    14941 cggcctcctc gggcgcaagt ccgggaaggg cttcttcgac tacgccaagt aaacagcgcc
    15001 gcgcgagcgg aaaggacgac ccgacatgga aaagagtttg attcgcctcc ggatgagctc
    15061 ccacgacgcc cactacggcg ggaacctcgt cgacggggcc aagatgctcc agctcttcgg
    15121 cgacgtcgcg acggagctcc tcatcctcca tgacggggac gagggcctct tcgcgggcta
    15181 cgagaaggtc gagttcctcg cccccgtctt cgcgggcgac tacatcgagg cctccggcga
    15241 gatcgtcagc gtggggaact cctcccgcaa gatgaccttc gaggcccgga aggtcatcgc
    15301 cccccggccc gacctgaacg actcggccgc cgacctcctt cccgagcccg tcgtcgtctg
    15361 cagggccgtc ggcacctgcg tcgttcccaa ggccaagcag cgcaagagct agggtttccc
    15421 tagtccgagg ctcgcgggat ccgcggggga ggcaaggcct ccctcggcgg gttccgacga
    15481 gcgtgcatga gtggtgattc agttctcaat acggcactgc cgaatacgag gtttccagga
    15541 tggatttcca ggcggacaag gagcacaggc tcctccaaga gatgtacagg gacttcgcgg
    15601 agacggaggt gaagcccctc gcgaaggaag tggacgagcg cgagctcttc cccgtggaga
    15661 cggtgaggaa gatggcgcgc tacggcttca tgggcattcc cttccccaag gaatacggcg
    15721 gctcgggcgg cgacaacctg gcctacatca tggccgtgga ggccctctcc aaggtctgcg
    15781 ggacgacggg cgtcgtcctc tcggcccact cctccctctg cgcggccccg atctacgagt
    15841 tcggcacgga ggagcagaag aggaagtacc tcgtgcccct ggccaagggc gagaagctcg
    15901 gcgccttcgg cctcaccgag cccaacgccg gcaccgacgc ctccggccag cagacgacgg
    15961 ccgagcccaa ggagggcttc tacctcctca acggctcgaa gctcttcatc acgaacgcgg
    16021 gctacgccga cctctacatc gtcttcgcga tgaccgaccg gaccaagggc acgaggggca
    16081 tctccgcctt catcgtggag aagggcatga agggcttctc catcgggaag aaggaggaga
    16141 agatgggcat ccgcggctcc tccacctgcg agctcgtctt cgagaatgtc gaggtcccgg
    16201 cggccaatct cctgggcaag gagggcggcg gcttcggcat cgccatgaag accctggacg
    16261 gcggccgcat cggcatcgcg gcccaggccc tgggcctggc ccagggcgcc atcgacgaga
    16321 cggtcgccta cgtgaaggag cggaagcagt tcgggaaggc cctggcgcag ttccagaaca
    16381 cccagttcca gctggccgac atgcaggcca ggacggacgc ggcccgcctc ctcgtctacc
    16441 gcgcggcggc gagcaaggac gcgaagctcc cctacgccgt cgaggcggcg acggccaagc
    16501 tcttcgcctc cgagaccgcg atggaggtga cgaccaaggc cgtccagctc cacggcggct
    16561 acggctacac gcgggagtac cccgtggagc gcatgatgcg cgacgcgaag atcaccgaga
    16621 tctacgaggg cacctcggag gtccagcgca tggtcatctc ggccgcgatg ctcaagtaaa
    16681 ggaagcgaag ccatgaacat cgtagtctgc gtgaagcagg tccccgacac cacggaagtg
    16741 aagatcgacc ccgccaccgg gaccctcgtc cgcgagggca tcccgagcat catcaacccc
    16801 gacgacaaga gcggcctgga ggccgccctg gagctcaagg accaggtagg cgcgaaggtg
    16861 accgtcctgt ccatgggccc gccccaggcg gagctcgccc tgcgcgaggc cctcgccatg
    16921 ggcgccgacg aggccatcct cctgtccgac cgcgccttcg gcggggccga cacctgggcc
    16981 acctcgacga ccctggccgc cgccctgcgg aagctcgact gggacctcgt ggtctgcggc
    17041 cgccaggcca tcgacggcga caccgcccag gtcggccccc agatcgccga gcacctcgga
    17101 atcccccacg tgagctacgt gagcgagctc aaggtcgagg gggaggccgt cgtcgtcaag
    17161 cgcgccttcg aggacggcta ccagcggatc agggccaagt ttccctgtct cgtcaccgcc
    17221 ctcaaggaca tgaacaagcc ccgctacatg tccgtgggcg gcgtcttcga cgcctacagg
    17281 aagacgatcc cggtctgggg cctcgccgac ctggacgtgg atcccgcgaa catcggcctc
    17341 aagggctcgc ccacgcgggt caagtcctcc tacaccaagg gcacgaagtc ggccggcaag
    17401 gtctacgagg tcgacccgaa ggaaggcgcg aggatcatcg tcgagcgcct gaaggaaaag
    17461 ttcatcatct aggggagggc gccgccccgc cttcaggcgg ccgcggcgcc agcacgaacg
    17521 caggagagcg actatatgaa cgtatcggaa tacaagggcg tcttcgtctt cgccgagcgg
    17581 cgcgacggcg ccgtgcagaa ggtggcctac gagctcctgg ggatgggccg cggcatcgcg
    17641 gacgccctgg gcgagccgct ggccgccgtc ctcctgggct cgggcctcaa ggactccgag
    17701 gccgccgagc tcatcgcctt cggcgccgac gaggtcatcc tcgtcgacca cccggacctg
    17761 ggcgtctaca tgaccgagcc ctacacgaag gcggtcgtca aggccatcgc ggagcggaag
    17821 ccgggcgtgg tcttcttcgg cgccacgacc atcggccgcg acctggcgcc ccgggtctcc
    17881 gcccgggtca agaccggcct caccgccgac tgcacggccc tggccgtgga ggacggcacg
    17941 cgcaacctcc tcatgacgcg gcccgccttc ggcggcaaca tcatggctac catcgtctgc
    18001 ccggagcacc ggccccagat gtccaccgtc aggccgggcg tcatgcggaa gctcgagcgg
    18061 gacggcgcga ggaagggcag ggtctcgagg ctcgcggtgg agatcgaggc gaaggacagg
    18121 aacgtcgaga tcctggaggt cgtctccgag aaggtcgcca ggaagaacat cgaggactcg
    18181 accgtcctcg tctccggcgg ccgcggcctc gggagccccg agaatttccg caagctcgac
    18241 ctcgtcgccg acgagctcgg cggcctcgtc tccgcctccc gcgcggtcgt ggacttgggc
    18301 tgggtcggcc aggaccgcca ggtcggccag accggcaaga ccgtcaggcc cgacctctac
    18361 atcgcctgcg gcatctccgg catgatccag cacatcgccg gcatggagga ctcgggcttc
    18421 atcgtcgcgg tcaacaagaa ctccggcgcg cccatcttcg agtacgccga cctggggatc
    18481 gtaaccgacg tgaaccgcct ggttcccgcc ctggccgagg agctgaaggc cgccaaggcg
    18541 gagcgctagc cctcctgccc gcgccctccg ccgcgccccg caggggcggc ggagggcccg
    18601 tcggctgacc ggcgcccggg ccgcggccga cgcgcttccg caagacccag gccgatcgcg
    18661 gccggcgcgc tcccgcaggg gccctcgccg ggccgcggct gtttacgcgt cccttctcct
    18721 ttgatatagt cgaaggatta tggcccgcta cgatttcgcg aacgagggga cctgctccga
    18781 gtcggtctcc ttcagcctgg acggggggcg tctcgcctcc gtccgctttt cgggcggctg
    18841 tccgggcaac ctcgccggga tagcccgcct ggtcgaggga ctcgaggccg aggaggccgc
    18901 gcgccgcctc gagggtatcc gctgcggatc caagtccacc tcctgtcccg accagctggc
    18961 caaagccata cggcgcgccc tcggcgcggc gacgacccaa gggagcgccc catgaacagc
    19021 caagccagat tccgcctcgg ccccatcgcg gccggggtcc tcctcctcgc cgtcctcctg
    19081 gtcgcgccga cggcctgcgc cacctccggc tacgacgcgg aggccgcctc gctctcgagc
    19141 tacgcggaga aggacggcgg cctcgtcctc cgcctcgcgc gggacggaag cctcgtctgg
    19201 ggcgcctggg aggacctctc cgcgaagcgc ttcggcctcg tcgagggcga ggccaggccc
    19261 gacggcggcc tcagcctgaa gctcttcggc gagagcgcct ccgccccccc cgcgaccctc
    19321 gagggccgct acgccgccgg cgagggccgc gtggagggga agctcgccgg cggcgacgcc
    19381 tcgcgcgacg tcgtcctccc gcgctcggcc gcccccgcca agctcgccct ctcgcgcctc
    19441 cacgccgagg agcggggcag cctggcggcc gaggccgagc cgacccgctt cgactacacc
    19501 tccatcgagc cggccgcgcc gaaggagttc ctggactggt acaggaagac cttccaggga
    19561 gggatggact cgggcgccgc catggcccgc gagaaggacg ccttcttcga ggacttccgc
    19621 tcgggctcgc aggccctcct cgaggccgcg gggcaggagg ccgtgcatcc ctggttctac
    19681 gacggcaggc agttcctcgc ctactcgaac ccgagcgtcc tcgtcctggg cctccgccgc
    19741 gcgaccttcg cgggcggcgc ccacggcatg cacggcgtgc gctacgcgat catcgacccc
    19801 gaggagccga ggatcctcgg cccggccgac ctcttcgtcc cgggctgcga cgaggccctc
    19861 tccggcctcc tcggagccaa ggcccgagag gccttcggcc tcgagcccgg ctcctccctc
    19921 tcccagggcg gcttcctcgt ggaggcgatc ccgccgacga acaacatgtt cgtctactcg
    19981 ggcggcatcg gcttccacta caacgtctac gaggtcgcgc cctacgcggc cggcgacatc
    20041 tggctctgcc tgcccttcga ggagctgggc ggagtcctcc tgcccggcgc cgcggagcgc
    20101 ctgggcttcc ccgcgggcaa ggaaggcgcg aagccgtgac cgaaagcctg gagatgtacc
    20161 tggagacgat cagcctcctc cacgaccgga gcaggatagc gcgcgtcacg gacatcgcgg
    20221 ccgagctcgg cgtctccaag ccctccgtgc acaccgccct ccacgagctc gagcgccgcg
    20281 gcctcatcga gcacgagcac tacggcgagg tcttcctcac gcagagcggc aaggccgcga
    20341 gcgccgccat ccgcgagcgc cacggcctcc tcaccgcctt cctgcgcgac ttcctgggcg
    20401 tctcgccggc cgtcgccgag caggacgcct gccggatgga gcacatcctc tccgaggaga
    20461 cgatggcgcg catcgcggag cggacgcgcg gcgcggccgc cgccggccgg ggcggctccg
    20521 cgcccggcgc cgcccagcct ggcccggccg agcctgccga cgccgcgcgg ccctgatccc
    20581 ctgaaggctg tccagccggg cgacgccatt tcgggcgccg cccggcagcc ctagatccct
    20641 cgccggacga tagggcgccg cttcgtcctg gaa
//
LOCUS       DAOOSJ010000014        19737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_1895_length_19737_cov_5.900620, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000014 DAOOSJ010000000
VERSION     DAOOSJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 19737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19737
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1895_length_19737_cov_5.900620"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLG14_01620"
     CDS             <1..1062
                     /locus_tag="PLG14_01620"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HOX47855.1"
                     /translation="GRDESRERPAVDPSRSYHNSAFKDKKRLVELKQAKNLTISLAFP
                     TLNEEATIAKEVLVIKTELMDRFPLIDEIAVIDSGSTDKTRETARRFGAKVFDSAAIL
                     PKYGSFRGKGENLWKSLYVLKGDIIVWIDADISNINPKFLYGLVGPLLEDDGIGYVKA
                     FYKRPIRTATGLTPSGGGRVTEILVRPLFSLFYPELAELVQPLSGEYAGRRSILERLS
                     FSVGYGVEVGHLIDLFHMFGPSILAQVDMEERIHRNQSIEALSRMSYGILATFLDRLE
                     KYGEISLLKELGKRHIALTADEETWKPVLSEISMVERPPMLTIPEYLARFPAAAAAPA
                     GSAGGEAPEDPQSPDAEGD"
     gene            complement(1277..2185)
                     /locus_tag="PLG14_01625"
     CDS             complement(1277..2185)
                     /locus_tag="PLG14_01625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX47856.1"
                     /translation="MTKQERLGLVRLLAEVSPRDLVAEAPPLRPDLAALAGARLPRRP
                     RAVLFDVYGTLVMSAAGGEPNLPGGADGQGGEGALGLLEEELSRAGFRGGARRFARDV
                     AGAIAADREERLAGTPHPEVDIEALVSRLLGEAAPATARRAALLLEASQNPCAPMPGA
                     LELLARLRASGLRLGLVSNAQFYTPLLLEALSGRSPEELGFEAGLQAYSFEGGIAKPA
                     EEPFLRAAGPLLAGGLSPGEILVVGNSSANDIAPARRLGFMAALFAGDARSFRPTAPG
                     SPGARPDTVLRGLDGLEAAFPPSDES"
     gene            complement(2191..3651)
                     /locus_tag="PLG14_01630"
     CDS             complement(2191..3651)
                     /locus_tag="PLG14_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47857.1"
                     /translation="MKVAMLHYHLRRGGVGSVMHRQAAGLALTEDPPELAFVVGAAPE
                     GPSSAPVFVVPGLDYDGFTGADASDLEAGAERLAAAIEEALSRAFPGGCDLLHVHNPL
                     LRKNARLLGALRILQSRGYALLVQEHDFAEDFRPDVYDSAWPYPESCDYATINSRDRD
                     SLVAAGLDPRHVHLLPNPVADDGEFSPRGGFAAEAAKGRRTVLYPVRAIRRKNLGEAL
                     LLSLFLPESAELAVTLPPTSEADWPGYRAWKAFAAKEGLPVRFEVGEGAPLAALYGRS
                     FAAVTTSVKEGFGYSYLDPLTRGIPVLGRRIPHIARDFSEQGIEFPGLYDSLLTPVAA
                     RERKGPRRAADGRLARFREVFGPAFGKAGVARLEALLEALPRRFEGDLLDFGALDPRS
                     QASLLARIKGDGAFAAEVVRLNPALERLASQAPSAEEALLRREAVARAYSERGCGELL
                     AAAYGQALRREASGRVDKAALLERYLQPSAFFLSAS"
     gene            3771..>5078
                     /locus_tag="PLG14_01635"
     CDS             3771..>5078
                     /locus_tag="PLG14_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX47858.1"
                     /translation="MPERDRGAVRDDGPSEGRGRTSLERLSQALGELYPGRGPELLPR
                     LLSLVETYRKKNEGVHGASGSRGGEAARDRGVAAGDRDAAARAAAPPRFRAGDAVLIA
                     YGDMLRGGEAPIATLAGFCERRLAGRFSHLHLLPFFPSSSDEGFSVTDFGSVDPRLGD
                     WSHVERLGRSFGLAFDLVLNHASAKGDWFRGFLAKREPYDRFFVARPEGFDASRVFRP
                     RTHPLHTRFRRDDGSEVLAWTTFSPDQVDLDYSEPEVFLAMVETALSYAARGARILRL
                     DAVAFAWKEDGTDCLDRPRVHALVRLLRAVLDEAAPGVSLLSETNLPQAVNDSYFGKG
                     DEAQLAYNFALPPLALHAFVGGRTGYLASWAAGLPGPEPWRSYVNFLSSHDGIGLTPA
                     RGLLPPAELERLVAAAKERGSLVSERSTPEGPVPYELNTTFLDA"
     assembly_gap    5079..5178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5179..5586
                     /locus_tag="PLG14_01640"
     CDS             <5179..5586
                     /locus_tag="PLG14_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47859.1"
                     /translation="AFDPASPQLVLSPDGSGKILAADQGGNGGGPCAGAAAGSAEGGR
                     LPDGPSPDGPVFALLRGRGEGAVLAAVNVSETPASCVLPRGFRPRGSPFDPAAGAGAA
                     GGEGPRLDGQSLVLPPLGAAWLDGSLEGDGRCI"
     gene            5577..6857
                     /locus_tag="PLG14_01645"
     CDS             5577..6857
                     /locus_tag="PLG14_01645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX47860.1"
                     /translation="MYLTKVNRIRRVGFVSTRFKGFDGVSLETAKWAAVLERMGFECF
                     YYCGQSDRPPERTMVAEEAYFGHPEVAALQAKCFGRTSRDEWITGEVHRLRQVLKKSL
                     YAFIESFKLDLLVVENALAIPMHIPLGLAITEVLAETGMPAIGHHHDFSWERQRFIMS
                     GVDDYLDMAFPPRIHAMNHVVINTEARTQLGHRRGLSSIVIPNVHDFSAEPRGVDDYN
                     RTLKADLGMAEGDLLFLQPTRVIARKGIEHAIELAGRLKDRRVKLLVSHQERDEGSDY
                     FRRILDYAGMLGVDLVAGGSLLGTSRGLTAEGKKVYDLWDCYANADLVTYPSTFEGFG
                     NAFLEAVFCRKPLMVNRYSIFESDIEPIGFQAALIDNYVTDDTVRHVRRLLDEPELRE
                     ATVARNFELGKKYFSYELLEQRLKYVIMNFGAAS"
     gene            6872..7387
                     /locus_tag="PLG14_01650"
     CDS             6872..7387
                     /locus_tag="PLG14_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX47861.1"
                     /translation="MDLFEAIEKRTSVRRYGTRRLSGAEWDRLLRAAMAAPSAVDRRP
                     WDFVVVEERKTLEALAAALPYAKMAAGAAGAVVVCASPDRAFQGSREFALLDATAALE
                     NLLLAATGLGLGAVWTALYPEADREAAARSILGIPADVIPLALVPVGEPAGPAEPKDK
                     FDPARIRRERW"
     gene            7428..7919
                     /locus_tag="PLG14_01655"
     CDS             7428..7919
                     /locus_tag="PLG14_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOX47862.1"
                     /translation="MIEVFLEAAAGSRVKGRYDERSLERVGERRLLLPYPCAYGFVLG
                     TSGPDGEALDCFVLGAGDAAEGARVACEPFTLLEFVEDGEEDHKLVALAPGAREPEPE
                     ALRLTLAAFMTGVFAAWPEVEVRVGRLLPREAAAARLEAAREAARTATREGSQRRSPG
                     PPG"
     gene            8175..10199
                     /gene="fusA"
                     /locus_tag="PLG14_01660"
     CDS             8175..10199
                     /gene="fusA"
                     /locus_tag="PLG14_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOX47863.1"
                     /translation="MDLRKTRNIGIMAHIDAGKTTTTERILYYSGKTYKIGEVDDGEA
                     TMDWMDQEQERGITIQSAATTTYWRGHQVNIIDTPGHVDFTAEVERSLRVLDGAVAVF
                     SAVDGVEPQSETVWHQADRYHVPRLAYVNKMDRLGADFDAVLEDMRKKLGARPVAVNA
                     PIGSGASFEGVVDLVAMEELRFAEDGNEIERSPVAPGRAEAAKALREALIDALSEGSD
                     EITSLFLEGKDVPADLIRAELRKATIGRRLVPALCGASRRNVGVQPLLDAVVDFLPAP
                     DEVPPAKAFHPKKEEEADVPCDAKGYPLGLVFKVQYDREAGPLCYVRMYSGSMKNASV
                     VFNATKKKRERVTRLLRMHANKSEPLDSVDAGDIAVFVGMKLAQTGDTIGTEGWPVLL
                     EKMHFPEPVITVAIEPQTLSDREKLQETLDILSKEDPTFTSKENEETGQLVISGMGEL
                     HLDVLVTRIIRDFKIKAKIGNPQVSYRESVSQAVERSERFEKVVAGKVNAAGLKLRLE
                     PRKRGEGNLYEKALRAPQVPEEIFEAIERGITGAFAGGIQYGYPAVDIGVKLLGVEYD
                     ELTATPFAYEACANMAYDAACREAGPVLLEPIMKVDIATPKEFLGEAMSLVSQRGGLI
                     HGSETKAASETIHAEAPLEAMFGFTTSLRSVTQGRASFSMEFSHFQPKRQ"
     gene            complement(10469..11056)
                     /locus_tag="PLG14_01665"
     CDS             complement(10469..11056)
                     /locus_tag="PLG14_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47864.1"
                     /translation="MDFVAFAIDESLVSDAERLAFEAFPRLVAAKTAQARAAEALVFA
                     DIIGNPGEFAQYVQGSSGDRLARIDRLLRIFRENVELLVHKTWVEKSDDKRKGKLLEE
                     LQAFERDFRDGSIASAFRRFVALARSLALLLFGGQSRADDFLHYCFRIDPKLGLFFWY
                     VGELEVQARESEAGRVSDELLRTETLIGIYVLSSF"
     gene            11331..13649
                     /locus_tag="PLG14_01670"
     CDS             11331..13649
                     /locus_tag="PLG14_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOX47865.1"
                     /translation="MNDIKILPEEAMGLGFVPPEQLAPGEDEYRIRDEQLRALGRDPK
                     SYRRLRPFELEILVKNDNTCRDWNQLLVTDPFAPELVKNSAFAGLVRLGRLERAVIEH
                     HDTRAPVGIYRSRLVACDVGDNCAILDCSSIAHYIVGDGSMLVNNDEIEASNHAKFGN
                     GVVMEGEPEEVRIELDLMNEAGGRSVLPFDGMLPADAYLWARRRGDPRLLSAFKAMTE
                     SMFDLRRGRYGEIGHHSVLKSNGIMKDVKIGPCAYIKGANKLKNLTVNSSEEARTQIG
                     EGVELVNGIIGYGCRVFYGCKAVRFVMGDNSALKYGARLIHSVLGENSTVSCCEILNN
                     LIFPAHEQHHNTSFLIASLVRGQSNMAAGATIGSNHNSRANDGEIEAGRGFWPGLSTS
                     VKHSSRFASFCLLAKGDYRYELDLRLPFCLVDDDRARERLVLVPAYWWTHNLYALLRN
                     ESKFESRDRRRDKALVLEYSPFAPDTAEEIWEAMRLLEDWAGRPLGAAAGAGGTAAAG
                     GAADGAAVGPAPDEILARGVENSGRPVVVVKPARACKAYREMLLWYAAGTVADFAESL
                     AAGLPAGGAGLFEALAERLGGPEAGAAAASGPEAWENLGGQLVPAAKLEALLSRAAAG
                     KIADWKEMHTEYARLAAEYPLDKARHAWATLARLYGGASADRADGASPARRGAATRAE
                     EAPGHRAESLQAADLLKAFEDYSRLSSFVEEEVYATRKKDYDNRFRRATFRSDAEYLA
                     VMGRPEDNPFVKRTRRDLAARRARLEELSGRL"
     gene            complement(13887..14978)
                     /gene="serC"
                     /locus_tag="PLG14_01675"
     CDS             complement(13887..14978)
                     /gene="serC"
                     /locus_tag="PLG14_01675"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOX47866.1"
                     /translation="MKRVINYSAGPAAIPLEVLEQARDEMLDWNGTGCSVMEVSHRGK
                     EYDALHMETMDLFRELAGLPKDYHVLFCTGGASTQFLMLPMNYLGAGKEADYVVTGNW
                     SKAAVKEAKRYGKISHPANMELEGEKFVAIPKQGDLKLNPAAEYVHYTSNNTVEGTEW
                     SYIPDAKGKPLVCDMSSDIFSRPLDFTKFSMIYAGAQKNLGPSGVTVVAIKDDFLAKA
                     HDDLPTMMSYKVFKEKFSMHNTPPCFSIYILNLVMKWLKKNGGLAAMGKINDQKQKLL
                     YAAVDGSKGFYKGLADVDSRSWMNIDFKLPTPELDDAFIKAAKAAGIVQTKGYRSMGG
                     IRLSAYNAVSLKDVETTVGFMKDFQAKNA"
     gene            complement(14989..15861)
                     /locus_tag="PLG14_01680"
     CDS             complement(14989..15861)
                     /locus_tag="PLG14_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOX47867.1"
                     /translation="MVILLADAFAPDLPARLKPFGEVTADVARVAEAEVIVVRSKTKV
                     DKAMIDKAPKLKYVIRGGVGVDTIDVEYAKSKGIAVDNTPEASSLAVAELAFALMIAM
                     PCHVAEADASMKQGKWLKKELERTELHGKTLGLVGIGRIARELAARAKAFGMKVIAYD
                     KYVEKSDAAEMTTLDEVLAKSDYISLHTPLTDETKGMINAAAIAKMKKGAYVINTCRG
                     QVIVEEDVAAALASGQLGGYGADVYYKEPPEGSPLLKAPRAVLTPHLGASTEENLLRL
                     GDCIVERIQKYAKK"
     gene            complement(16163..16867)
                     /locus_tag="PLG14_01685"
     CDS             complement(16163..16867)
                     /locus_tag="PLG14_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX47868.1"
                     /translation="MESLEAYVERYGLAELLSADLLGALSLVATEPGGLILRAGEAAD
                     RLLFFVEGRAKVYSLLENGKSLLASFYEPFEVLGDAELFAYARWTLNVEAITATACLS
                     LPAEAARKAADRNGRLFAFLCGRLGRKLATFNVQSAINLRYPVENRLASYLLAVLELE
                     ERAGAAGPGAAESGTAEYGTDDLSELADSLGTSYRQLSRVVRRFREEGILEDRRGRIR
                     VLSRERLEPLAREIYL"
     gene            16962..17840
                     /locus_tag="PLG14_01690"
     CDS             16962..17840
                     /locus_tag="PLG14_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX47869.1"
                     /translation="MAYFIAAPFLGAFITLMNRLNSDLAAVVGTYASSLVIHLAGLGA
                     VSLVLLRRPEPRPARRPPLLRYSGGLVGLATVFLCNAVYGALDASLAVALALLGQMLG
                     SILVDATGFLGRTKRPFTARSLPGLALALAGAAALSGGGRAAPGLIFLAVAAGALPLV
                     SITLNARLAQDIGLFRGVRANYLVGLAATLLLAPLFGRAGLAGLAAAGSAKPLQLAGG
                     GILGVALVAGTNLVFPRLPALQASILMFSGQLLSGLAVDAASGAAIQARTPLGLLAVL
                     AGLALDAGLKALPTRT"
     gene            complement(17893..18885)
                     /locus_tag="PLG14_01695"
     CDS             complement(17893..18885)
                     /locus_tag="PLG14_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOX47870.1"
                     /translation="MSPPPAPILCVCLNPVIQKTLLFDRLLPGEVNRSGEHRVDAAGK
                     AVDVARVLGQTGRPAVLLSQAGGANRDWFLSLCARDGLDLRAVESGSEIRFCHTLVER
                     GALRTTELVEEAEPVAEGTGGRLLGELDRLLPACRAVVVSGTKAAGLEEDLVPEIARR
                     VRAAGLLLVLDVKGPDLLRALEHRPAVAKPNLSEFLATWPLPGRAGAGALHGAAPSGA
                     GAAAEGEAAAPADDAAGLRAHAAAVASELHGRFGTSLVLTRGHEPTWWWDGRSLREEA
                     VRPVQALNPTGSGDAFTAGLAAVLAEGGSLAAAVAEGTRLGALNAASLRPGSIL"
     gene            complement(19117..>19737)
                     /locus_tag="PLG14_01700"
     CDS             complement(19117..>19737)
                     /locus_tag="PLG14_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47871.1"
                     /translation="ATAFLGMVVNPGDPYEFQRVDSGELYSGAGDAGEWIVGYAGATF
                     SDATLDEIALADKTHFAGMTVAGVPFPDATLRPIREAVVAAHEELKSLKLDNGAPMVG
                     YKAINSVGTVTKNVVYNGLEPYLGQLYMPDMDYDNGGDGWDPVDFENGTMKTSDGDLI
                     GFIPDPSNSSAIKHCQANLSSFISGFPASAWRNNTAFRSIYSDWTP"
BASE COUNT         3040 a         6935 c         6854 g         2808 t
ORIGIN      
        1 ggccgagacg aaagccgtga acgaccggcc gtcgatccct cgcggtccta ccacaacagc
       61 gccttcaagg acaagaagcg cctcgtcgag ctcaagcagg ccaagaacct gacgatctcc
      121 ctcgccttcc cgacgctcaa cgaggaggcg accatcgcca aggaggtcct cgtcatcaag
      181 accgagctca tggaccgctt ccccctcatc gacgagatcg cggtcatcga ctcgggctcc
      241 accgacaaga cccgggagac ggcgaggcgc ttcggagcga aggtgttcga ctccgcggcg
      301 atcctcccga aatacgggag cttccgcggc aagggcgaga acctctggaa gagcctctac
      361 gtcctcaagg gcgacatcat cgtgtggatc gacgcggaca tcagcaacat aaacccgaag
      421 ttcctgtacg gcctggtcgg ccccctcctg gaggacgacg ggatcggcta cgtgaaggcc
      481 ttctacaagc ggccgatccg cacggcgacg gggctcaccc cgagtggcgg cggtcgggtg
      541 accgagatcc tggtcaggcc gctcttctcc ctcttctacc cggagctggc cgagctcgtg
      601 cagcccctga gcggcgagta cgcgggaagg cggagcatcc tggagcggct gtccttctcc
      661 gtcggctacg gcgtcgaggt cggccacctc atcgacctgt tccacatgtt cgggccctcg
      721 atcctcgccc aggtggacat ggaggagcgg atccaccgca accagagcat cgaggcgctc
      781 agccggatgt cctacggcat cctcgccacc ttcctggacc gcctggagaa gtacggagag
      841 atctccctgc tcaaggagct cggcaagcgg cacatcgccc tgaccgccga cgaggagacc
      901 tggaagccgg tcttgtcgga gatctccatg gtcgagcggc cgccgatgct cacgataccc
      961 gagtacctcg cgagattccc cgccgcggcc gcggctcccg ccggctcggc tggcggggag
     1021 gcgccggaag atccccagtc gccggacgcg gaaggggatt gagcaggaaa ggcttcgcgc
     1081 gctccggccg ccatgactcc gaaggcccag cccccgaggg ctcggccccg cccgacggcg
     1141 cggcggccaa gccgcgaggc ctccggtggc tcggctccgc ccggcggcgc ggaggctaag
     1201 cggcgcggcg gctatgcggc gcggcgacta tacggcgcgg cggcgcgtca tccagcactc
     1261 ggcgcccgcg gacccttcag gattcgtcgg agggagggaa ggccgcctcg aggccgtcca
     1321 ggccgcgaag gaccgtgtca ggccgcgcgc cgggcgatcc cggcgcggtg ggcctgaagg
     1381 agcgggcgtc gccggcgaag agggcggcca tgaagccgag gcggcgcgcc ggggcgatgt
     1441 cgttggccga gctgttgccg accacgagga tctcgccggg ggaaaggcct ccagcgagaa
     1501 gcggcccggc cgcccggagg aagggctcct ccgcgggctt cgcgatccct ccctcgaagg
     1561 agtaggcctg cagtccggcc tcgaagccga gctcttcggg cgaccgcccc gacagggcct
     1621 cgaggaggag gggcgtatag aactgcgcgt tcgacacgag gccgagccgc aggccggagg
     1681 cgcggagcct ggccaggagc tccagggccc cgggcatcgg cgcgcaggga ttctgcgagg
     1741 cctcgaggag gagggccgct cgccgcgcgg tcgccggagc ggcctccccg agaaggcggg
     1801 agaccagggc ttcgatgtcc acctcggggt gcggcgtgcc cgcgagccgc tcctccctgt
     1861 cggccgcgat ggcgcccgcc acgtcgcggg caaagcggcg cgcgccgccc cggaagcccg
     1921 cgcgggagag ctcttcctcg agcaggccca gggccccctc gccgccttgc ccgtccgcgc
     1981 cgcctggcag gttgggctct cctcccgccg cgctcatgac gagcgtcccg tagacgtcga
     2041 agaggaccgc gcgcggccgg cggggaagcc gcgcgccggc gagggccgcg aggtcgggcc
     2101 tgaggggggg cgcctcggcg acgagatccc ggggagagac ctccgcgagg agcctgacga
     2161 gtccgagccg ctcttgcttc gtcatcggct tcatgaggcg gacaggaaga aggccgaggg
     2221 ctggaggtag cgctcgagaa gtgccgcctt gtcgacgcgt ccggaagcct cgcggcggag
     2281 ggcctggccg taggccgcgg cgaggagctc gccgcagccg cgctccgagt aggcccgcgc
     2341 gacggcctcg cgccgaagga gagcctcctc ggccgaagga gcctggctcg cgagtcgctc
     2401 aagggcggga ttgaggcgga cgacctcggc ggcgaaggcg ccgtcgccct tgatccgggc
     2461 gagcaaggaa gcctgggacc gcgggtccag ggcgccgaaa tcgaggagat cgccctcgaa
     2521 cctccgcggc agggcctcga ggagggcctc cagccgggcc acgccggcct tgccgaaggc
     2581 tggtccgaag acctcgcgga accgcgccag cctcccgtcg gcggcccgcc gcgggccttt
     2641 cctttcgcgg gcggcgaccg gcgtcaagag ggagtcgtag aggccgggga actcgatgcc
     2701 ctgttcggag aaatccctcg cgatatgggg tatccgcctt ccgagcacgg ggatgccccg
     2761 cgtcagggga tcgagatagg agtagccgaa gccttccttc acggaggtcg tcacggcggc
     2821 gaaggagcgc ccgtagagcg ccgcgagcgg cgcgccttcg cccacctcga agcggacagg
     2881 caggccttcc ttggcggcga aggccttcca ggcccgatag ccgggccaat cggcctcgct
     2941 cgtcggggga agggtgaccg ctagctcggc gctctcgggc aggaacagcg aaaggaggag
     3001 ggcctcgccc aggttcttcc gcctgatcgc ccgcacgggg tagaggacgg tccttctgcc
     3061 cttggcggcc tccgccgcga agccgccgcg cggggagaat tccccgtcgt cggcgacggg
     3121 attgggaagg aggtggacgt gccgggggtc gaggcccgcg gcgaccaggc tgtcccggtc
     3181 gcggctgttg atcgtcgcgt agtcgcagga ttcgggatag ggccaggccg agtcgtagac
     3241 atcgggcctg aagtcctccg cgaaatcgtg ctcctgcacg aggagcgcgt agccgcgcga
     3301 ttggaggatc ctcagggccc cgaggaggcg cgcgttcttc ctgagcaggg ggttgtggac
     3361 atggaggagg tcgcagccgc ccgggaaggc gcgggaaagg gcctcctcga tcgcggcggc
     3421 gagcctctcg gcgcccgcct ccaggtcgga cgcgtccgcg cccgtgaagc cgtcgtaatc
     3481 gaggcccggc acgacgaaga ccggcgcgga agagggaccc tcgggggcgg cgcccacgac
     3541 gaaggccagc tccggcggat cctccgtcag cgcgagaccg gcagcctgcc ggtgcatgac
     3601 cgagccgacg ccgccccggc gcagatgata atggagcatc gcgaccttca tgagtccatc
     3661 atagaacaca tcgttccgaa atcaaaatac cgtccgggag ccggaccgcg ccggcgtccg
     3721 cccggcttcg aacattgaca gacggagcgg cggacgctga tacttgagcc atgcccgagc
     3781 gagaccgcgg agccgtccgc gacgacggac catcggaggg aaggggaagg acaagcctcg
     3841 agcggctctc ccaggccctc ggagagctct accccgggcg cgggcccgaa ctcctgcccc
     3901 gcctcctctc cctcgtcgag acctacagga aaaaaaacga aggggtccac ggcgcgtccg
     3961 gcagccgcgg gggggaagcc gcccgcgaca ggggcgtggc agccggcgac agggacgcgg
     4021 ccgcccgagc cgccgcgccc ccgcggttcc gggcgggcga cgccgtcctc atcgcctacg
     4081 gcgacatgct ccgcggaggc gaggccccca tcgcgaccct ggccggcttc tgcgagcgga
     4141 ggctcgcggg ccgcttcagc cacctgcacc tccttccctt cttcccctcc tcctccgacg
     4201 agggcttctc cgtgacggat ttcggctccg tcgatccccg cctgggcgac tggagccatg
     4261 tcgagcggct cgggcggagc ttcggcctcg ccttcgacct cgtgctcaac cacgcctcgg
     4321 cgaaagggga ctggttccga ggcttcctcg cgaaacgcga gccctacgac cgtttcttcg
     4381 tggccaggcc ggagggcttc gacgcctccc gggtcttcag gcccaggacc caccccctcc
     4441 acacgcgctt caggcgggac gacggctcgg aggtcctggc ctggacgacc ttcagccccg
     4501 accaggtgga cctggactac tcggagccgg aggtcttcct cgcgatggtc gagacggcgc
     4561 tctcctacgc cgcgcggggg gcgaggatcc tcaggctcga cgcggtcgcc ttcgcgtgga
     4621 aggaggacgg caccgactgc ctggaccgcc cccgggtcca cgccctcgtg cggctcctcc
     4681 gcgccgtcct ggacgaggcg gcgcccggcg tctccctcct ctccgagacc aaccttcccc
     4741 aggccgtcaa cgattcgtac ttcgggaagg gggacgaggc ccagctggcc tacaacttcg
     4801 cgctgccgcc cctggccctc cacgccttcg tgggcggccg cacgggatac ctggcctcct
     4861 gggccgccgg ccttcccggg cccgagccct ggaggagcta cgtcaacttc ctctcgagcc
     4921 acgacggcat cggcctcacg ccggcgcggg gcctcctccc gcctgcggag ctcgagcggc
     4981 tcgtggcggc ggcgaaggag cgcgggagcc tcgtatcgga gcggtcgacc ccggaagggc
     5041 ccgtccccta cgagctcaac acgaccttcc tggacgcgnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnngc cttcgatccc gcctcgcccc agctcgtcct ctcccccgac
     5221 ggctcgggaa agatcctcgc ggcggaccag ggcgggaacg gtggaggccc atgcgcaggg
     5281 gccgcggcag gaagcgccga gggcggccgc ttgcccgacg gcccgtcccc cgacggcccg
     5341 gtcttcgccc tccttcgcgg gaggggcgaa ggcgcggtcc tggccgcggt gaacgtctcg
     5401 gagacgcccg cgtcctgcgt cctgccgcga ggcttcaggc cgcgcggctc gcccttcgac
     5461 ccggcggccg gagcgggagc ggcgggcggc gagggccccc gcctggacgg gcaaagcctc
     5521 gtcctgcccc ccctgggcgc ggcctggctg gacggatccc tggaaggaga tgggcgatgt
     5581 atctgaccaa agtgaacagg atccgccggg tgggcttcgt ctccacgagg ttcaagggct
     5641 tcgacggcgt ctccctggag acggccaaat gggcggccgt cctggagcgg atgggcttcg
     5701 agtgcttcta ctactgcggc cagtcggacc ggccgcccga gaggacgatg gtcgcggagg
     5761 aggcctactt cggccacccc gaggtcgcgg ccctccaggc gaagtgcttc ggaaggacca
     5821 gccgggacga gtggatcacc ggcgaggtgc accgcctccg ccaggtcctc aagaaaagcc
     5881 tctacgcctt catcgagagc ttcaagctcg acctcctcgt cgtcgagaac gccctcgcga
     5941 tacccatgca catacccctg ggactcgcga tcaccgaggt cctggcggag acgggcatgc
     6001 ccgccatcgg gcaccaccac gacttctcct gggagcgcca gcgcttcatc atgagcgggg
     6061 tcgacgacta cctcgacatg gccttccccc cgcggatcca cgcgatgaac cacgtcgtca
     6121 tcaacaccga ggcgcggacc cagctggggc accggagggg actctcgagc atcgtcatcc
     6181 cgaacgtcca cgatttctcc gcggagcctc ggggcgtcga cgactacaac cgcacgctca
     6241 aggccgacct gggcatggcg gagggcgacc tcctcttcct ccagccgacc cgcgtcatcg
     6301 cccgcaaggg catcgagcac gcgatcgagc tcgcgggccg gctcaaggac cgccgcgtca
     6361 agctcctcgt gagccaccag gagcgggacg agggctcgga ctatttcagg cgcatcctcg
     6421 actacgccgg gatgctcggc gtcgacctgg tcgcgggagg ctccctcctc gggacctcgc
     6481 ggggcctgac ggccgaggga aagaaggtct acgacctctg ggactgctac gcgaacgccg
     6541 acctcgtgac ctacccctcc accttcgagg gcttcggaaa cgccttcctg gaggccgtct
     6601 tctgcaggaa gcccctcatg gtcaaccgct actcgatctt cgagagcgac atcgagccca
     6661 tcggcttcca ggccgccctg atcgacaact acgtcaccga cgacaccgtc cgccacgtcc
     6721 ggaggctgct cgacgagccc gagctccggg aggcgacggt cgcccgcaac ttcgagctcg
     6781 ggaagaagta cttctcctac gagctgctcg agcagaggct caagtacgtc atcatgaact
     6841 tcggcgcggc gagctgaaga cgggagggac catggacctg ttcgaggcga tcgagaagag
     6901 gacgagcgtg cggaggtacg gcacccgccg cctttcgggg gccgagtggg acaggctcct
     6961 ccgcgcggcc atggccgccc cctcggccgt ggaccggcgc ccctgggact tcgtcgtcgt
     7021 ggaggagagg aagaccctgg aggccctggc cgccgccctg ccctacgcga agatggccgc
     7081 cggcgcggcc ggcgccgtgg tcgtctgcgc ctccccggat cgcgccttcc agggctcccg
     7141 cgagttcgcc ctcctggacg cgacggcggc cctggagaac ctccttctcg cggcgacggg
     7201 cctgggcctg ggcgcggtct ggacggccct ctaccccgag gccgaccgcg aggccgccgc
     7261 gcgctcgatc ctgggcattc ccgcggacgt gatccccctg gccctggtcc cggtcggcga
     7321 gccggcgggc cccgccgagc ccaaggacaa gttcgatccg gcgcggatcc ggcgggagcg
     7381 ctggtgaggg cgaagcgccg cctacgccgg cgcggacgga gcccccggtg atcgaggtct
     7441 tcctggaggc ggccgcgggc tcgcgggtga agggccgcta cgacgagagg agcctcgagc
     7501 gcgtcggcga gcgaaggctc ctcctcccct acccctgcgc ctacggcttc gtcctcggga
     7561 cctcgggacc cgacggcgag gccctggact gcttcgtcct cggcgctggg gacgcggccg
     7621 agggcgcgcg ggtagcctgc gagcccttca ccctcctcga gttcgtggag gacggcgagg
     7681 aggaccacaa gctcgtcgcg ctcgcgccgg gggctaggga gcccgagccc gaggcgctca
     7741 ggctgacgct cgccgccttc atgacgggag tcttcgcggc ctggcccgag gtcgaggtcc
     7801 gggtcggccg cctcctgccg cgggaggccg cggccgcccg tctcgaggcc gcgcgcgagg
     7861 cggcgcgaac ggcgacgcgc gagggatcgc agcggagatc cccggggccg ccgggctgaa
     7921 ggcgcgcgcg gggctcccgg agggagaccc gcgccccggg acggccccga agggggcgga
     7981 cccgggatcg gagcggagag cccgacggcg ccgccggcct gagtccgggc ggcggccgct
     8041 gtccggaaag gccggcggcg ccggcgcgcc cctccctcca agcattcctc cgccccgccc
     8101 cgtctccttg caaagcctgc gtttccttgt atatagttgc ggtgacaagc acgcactact
     8161 agggagcccc ttccatggac cttcgcaaga cccgcaacat cggcatcatg gcccacatcg
     8221 acgccggaaa gaccacgacc accgagcgca tcctctacta ctcggggaag acctacaaga
     8281 tcggcgaggt cgacgacggc gaggcgacca tggactggat ggaccaggag caggagcgcg
     8341 gcatcacgat ccagagcgcc gcgacgacga cctactggcg cgggcaccag gtcaacatca
     8401 tcgacacccc cggccacgtc gacttcacgg cggaggtcga gcgctccctg cgcgtcctgg
     8461 acggggcggt cgcggtcttc tccgcggtgg acggggtcga gccccagagc gagacggtct
     8521 ggcaccaggc cgaccgctac cacgtcccgc gcctggccta cgtgaacaag atggaccgcc
     8581 tgggcgccga cttcgacgcg gtgctggagg acatgcggaa gaagctcggc gcgaggccgg
     8641 tggcggtcaa cgcgcccatc ggctcaggcg cctccttcga gggcgtcgtg gacctggtcg
     8701 cgatggagga gctccgcttc gccgaggacg gcaacgagat cgagcgctcc cccgtcgccc
     8761 ccgggcgcgc ggaggccgcg aaggccctcc gcgaggccct gatcgacgcc ctctccgagg
     8821 gctccgacga gatcacctcc ctcttcctgg aggggaagga cgtgccggcc gacctgatcc
     8881 gcgccgaact ccgcaaggcc acgatcggcc gccgcctggt cccggccctc tgcggcgcga
     8941 gccgccgcaa cgtgggcgtc cagcccctcc tggacgcggt cgtggacttc ctgcccgccc
     9001 ccgacgaggt cccgcccgcc aaggccttcc accccaagaa ggaggaggag gccgacgtgc
     9061 cctgcgacgc gaagggctac cccctgggcc tcgtcttcaa ggtccagtac gaccgcgagg
     9121 cgggcccgct ctgctacgtg cgcatgtact ccggctccat gaagaacgcg agcgtcgtct
     9181 tcaacgcgac caagaagaag cgcgagcgcg tgacgcgcct cctccggatg cacgcgaaca
     9241 agtccgagcc cctggactcc gtggacgcgg gcgacatcgc cgtcttcgtc ggcatgaagc
     9301 tcgcgcagac gggcgacacg atcggcaccg agggctggcc ggtcctcctg gagaagatgc
     9361 acttccccga gcccgtcatc accgtcgcca tcgagcccca gaccctctcc gaccgcgaga
     9421 agctccagga gaccctggac atcctctcca aggaggaccc caccttcacg agcaaggaga
     9481 acgaggagac gggccagctc gtcatctccg gcatgggcga gctgcacctg gacgtcctgg
     9541 tcacgcggat catccgggac ttcaagatca aggcgaagat cggcaatccg caggtgagct
     9601 accgcgagtc cgtgagccag gccgtggagc gctcggagcg cttcgagaag gtggtcgcgg
     9661 gcaaggtgaa cgccgcgggc ctcaagctga ggctggagcc ccgcaagcgc ggcgagggca
     9721 acctctacga gaaggccctg cgggccccgc aggtcccgga ggagatcttc gaggcgatcg
     9781 agcgcggcat cacgggcgcc ttcgcgggcg gcatccagta cggctacccc gccgtggaca
     9841 tcggcgtgaa gctcctgggc gtcgagtacg acgagctcac cgcgacgccc ttcgcctacg
     9901 aggcctgcgc caacatggcc tacgacgcgg cctgccgcga ggcgggcccc gtcctcctgg
     9961 agcccatcat gaaggtggac atcgcgacgc ccaaggagtt cctgggcgag gcgatgagcc
    10021 tcgtctccca gcgcggcggc ctcatccacg gctcggagac caaggcggcc tccgagacca
    10081 tccacgccga ggccccgctg gaggcgatgt tcggcttcac gacgagcctc cgctccgtga
    10141 cccagggccg cgcgagcttc tcgatggagt tcagccactt ccagccgaag cggcagtagg
    10201 caggaacgtc gcgcgaatag gagaagccgc ccggccgggg cggcttcttt tgtcttgcgg
    10261 cgcgaacgcg gccctgggag ggagccatgg atggcgaccc gcgggcctcg ccggacgcgc
    10321 cgcgcgcgtc cggcggcgtc agggaagacg ggggccgcgg cggctcctag tcccgcgtgg
    10381 ccaaagcgaa agaaaccgcc acgatcgtag cggtttcttt gttcaggaga ggctggcttt
    10441 cggcgccggg ctcagcgcag ggcggggatc agaagctcga gagcacgtag atgccgatca
    10501 gggtctcggt ccggagaagc tcgtcggaga cgcggcccgc ctcgctctcg cgcgcctgga
    10561 cctcgagctc gccgacgtac cagaagaaaa gcccgagctt cgggtcgatg cggaagcagt
    10621 agtggaggaa gtcgtccgcg cggctctggc cgccgaagag gaggagggcc agggagcggg
    10681 ccagggcgac gaagcggcgg aaggcggagg cgatggagcc gtcgcggaag tcgcgctcga
    10741 aggcctggag ctcctcgagg agcttgccct tgcgcttgtc gtcggacttc tccacccagg
    10801 tcttgtggac gaggagctcg acgttctcgc ggaagatgcg gaggagccgg tcgatgcggg
    10861 ccaggcggtc gccggaggag ccctggacgt actgcgcgaa ttcgccgggg ttgcctatga
    10921 tgtcggcgaa gacgagggcc tcggccgcgc gggcctgcgc cgtcttggcc gcgacgagcc
    10981 gcgggaaggc ctcgaaggcc agccgctcgg cgtcggagac gaggctctcg tcgatggcga
    11041 aggcaacaaa atccatggcc tacgatgtcc cgaaggcctt gcctttgtca agcgggcggc
    11101 cttcccgcgc gggtccgggc cccgcggccc cgccgcggcg gcgcccgccg ccacgcgcgc
    11161 gaggcgcgcc ggcgcgcctc ggtccgcgcc ctcccgggcc ctgcggcccc gccgcgccgg
    11221 cgcgcggcgc tcgtcgctgg cggcctcccg cggcggctcc ccgttggacc gctccggagc
    11281 gcggcggccc ttgcgccgcg gcccccgcgg ggagtaggct cgcaggagcc atgaacgaca
    11341 tcaaaatact gccggaagaa gccatgggcc tcggcttcgt cccgcccgag cagctcgccc
    11401 ccggcgagga cgagtaccgg atccgcgacg agcagctcag ggccctgggc cgcgacccca
    11461 agtcctaccg ccgcctccgc cccttcgagc tcgagatcct cgtcaagaac gacaacacct
    11521 gccgggactg gaaccagctc ctcgtcaccg accccttcgc ccccgagctc gtgaagaact
    11581 ccgccttcgc cggcctcgtg cgcctgggga ggctcgagcg ggccgtgatc gagcaccacg
    11641 acacgagggc gcccgtgggc atctaccgct cgcgcctggt cgcctgcgac gtgggcgaca
    11701 actgcgcgat cctggactgc tcctccatcg cgcactacat cgtcggagac ggctcgatgc
    11761 tcgtcaacaa cgacgagatc gaggcctcca accacgcgaa gttcggcaac ggcgtcgtga
    11821 tggaggggga gcccgaggag gtgcgcatcg agctcgacct catgaacgag gcgggcggcc
    11881 gctccgtcct ccccttcgac gggatgctgc ccgcggacgc ctacctctgg gcgcgccggc
    11941 gcggcgaccc ccggctcctc tccgccttca aggcgatgac ggagtccatg ttcgacctgc
    12001 gccgcgggcg ctacggcgag atcggccacc actcggtcct caagtcgaac ggcatcatga
    12061 aggacgtgaa gatcggcccc tgcgcctaca tcaagggcgc gaacaagctc aagaacctga
    12121 ccgtcaactc gagcgaggag gcgcgcaccc agatcggcga gggcgtggag ctcgtcaacg
    12181 ggatcatcgg ctacggctgc cgcgtcttct acggctgcaa ggccgtgcgc ttcgtgatgg
    12241 gcgacaactc ggccctcaag tacggggcgc gcctcatcca ctcggtcctg ggcgagaact
    12301 cgaccgtctc ctgctgcgaa atcctcaaca acctcatctt ccccgcccac gagcagcacc
    12361 acaacacgag cttcctcatc gcgagcctgg tgcgcggcca gtccaacatg gccgccggcg
    12421 ccaccatcgg ctcgaaccac aacagccggg cgaacgacgg cgagatcgag gcgggccgcg
    12481 gcttctggcc gggcctctcc acctcggtga agcacagctc gcgcttcgcc tccttctgcc
    12541 tcctcgccaa gggcgactac cgctacgagc tcgacctgcg cctccccttc tgcctcgtcg
    12601 acgacgaccg cgcccgcgag cgcctcgtcc tcgtgcccgc ctactggtgg acgcacaacc
    12661 tctacgccct cctccgcaac gagtcgaagt tcgagtcccg cgacaggcgc cgcgacaagg
    12721 ccctcgtcct cgagtactcc cccttcgcgc cggacacggc cgaggagatc tgggaggcga
    12781 tgcgcctcct cgaggactgg gcgggcaggc ccctgggcgc cgcggccggc gcgggcggga
    12841 ccgccgcggc aggcggcgcc gctgacggcg cggccgtcgg ccccgcgccc gacgagatcc
    12901 tggcccgcgg cgtcgagaac tcgggccgcc ccgtcgtcgt cgtgaagccc gcgagggcct
    12961 gcaaggccta ccgagagatg ctcctctggt acgcggcggg gaccgtcgcc gacttcgcgg
    13021 agtccctcgc cgcagggctg cccgcgggcg gagccggcct cttcgaggcc ctggccgagc
    13081 ggctcggcgg ccccgaagcc ggggccgcgg ccgcctcggg ccccgaggcc tgggagaacc
    13141 tgggcggcca gctcgtgccg gccgccaagc tcgaggccct cctctcccgc gcggccgcgg
    13201 ggaagatcgc ggactggaag gagatgcaca cggagtacgc ccggctggcc gcggagtatc
    13261 ccctcgacaa ggccaggcac gcctgggcca ccctggcgcg gctctacggc ggcgcgtccg
    13321 cggaccgcgc cgacggagct tcgcctgcgc gccgcggcgc cgcgacgcgc gcggaggaag
    13381 cgcccggcca tcgcgccgaa agcctccaag ccgcggacct tcttaaggcc ttcgaggact
    13441 actcccgcct ctcctccttc gtcgaggagg aggtctacgc gacgaggaag aaggactacg
    13501 acaaccgctt ccgccgcgcg accttccgca gcgacgctga gtacctggcg gtgatggggc
    13561 gccccgagga caatcccttc gtgaagcgga cgcggcgcga cctcgccgcc cgccgcgcgc
    13621 ggctggagga gctctcaggc aggctgtagc ccggagcggc ggctcgggcg cccgcggaaa
    13681 agccgcggag cggcggccca agggtgcagg ccgtcgcggc ggtaccgcgg gcgaggcgac
    13741 ggcgcggccc gcaggcaagt cggcctcgcg gggagccgtc gcggcgaggc ccagggaacg
    13801 ccgcaagccc cctgtccatg aaaagcgaag aggccgcccg gcacgggcgg cctcttcata
    13861 ttgcgcggga agcgccggcg cgagggctag gcgttcttcg cctggaagtc cttcatgaag
    13921 ccgacggtcg tctcgacgtc cttgaggctc acagcgttgt aggcggagag gcggatgccg
    13981 cccatggagc ggtagccctt cgtctgcacg atgcccgcgg ccttggcggc cttgatgaag
    14041 gcgtcgtcga gctcgggggt cgggagcttg aagtcgatgt tcatccagga gcgggaatcg
    14101 acgtccgcga ggcccttgta gaagcccttg gagccgtcga ccgcggcgta gaggagcttc
    14161 tgcttctggt cgttgatctt gcccatcgcc gcaaggccgc cgttcttctt gagccacttc
    14221 atcacgaggt tgaggatgta gatggagaag cagggcgggg tgttgtgcat ggagaacttc
    14281 tccttgaaca ccttgtagga catcatcgtc gggaggtcgt cgtgggcctt cgcgaggaag
    14341 tcgtccttga tcgcgacgac ggtgacgccg gaggggccga ggttcttctg cgcgcccgcg
    14401 tagatcatgc tgaacttggt gaagtccagg gggcggctga agatgtcgga ggacatgtcg
    14461 cagacgaggg gcttgccctt ggcgtcgggg atgtaggacc actcggtgcc ctcgaccgtg
    14521 ttgttcgagg tgtagtggac gtactccgcg gcggggttga gcttgaggtc gccctgcttg
    14581 gggatcgcga cgaacttctc gccctcgagc tccatgttgg cgggatggct gatcttgccg
    14641 tagcgcttgg cctccttgac ggcggccttg gaccagttgc ccgtgacgac gtagtccgcc
    14701 tccttgcccg cgccgaggta gttcatcggc agcatgagga actgggtgct cgcgccgccc
    14761 gtgcagaaga ggacgtggta gtccttgggc aggccggcga gctcgcggaa gaggtccatg
    14821 gtctccatgt ggagggcgtc gtactccttg ccgcggtggg agacttccat gacggagcag
    14881 ccggtgccgt tccagtcgag catctcgtcg cgggcctgct ccaggacctc gagggggatc
    14941 gccgcggggc cggcgctgta gttgatgact cgcttcatgt tcctctcctt atttcttcgc
    15001 gtacttctgg atgcgctcga cgatgcagtc gccgaggcgg aggaggttct cctccgtgga
    15061 cgcgccgagg tgcggcgtga ggacggcgcg cggcgccttg aggaggggcg agccttcggg
    15121 cggctccttg tagtacacgt ccgcgccgta gccgccgagc tggccggagg cgagggcggc
    15181 ggcgacgtcc tcctcgacga tgacctgccc gcggcaggtg ttgatgacgt aggcgccctt
    15241 cttcatcttc gcgatggcgg cggcgttgat catgcccttc gtctcgtcgg tgaggggcgt
    15301 gtggagggag atgtagtcgg acttggcgag gacctcgtcg agggtcgtca tctccgcggc
    15361 gtcggacttc tccacgtact tgtcgtaggc gatgaccttc atgccgaagg ccttcgcgcg
    15421 ggcggcgagc tcgcgggcga tgcggccgat gccgacgagg cccagggtct tgccgtggag
    15481 ctcggtgcgc tcgagctcct tcttgagcca cttgccctgc ttcatggagg cgtcggcctc
    15541 ggcgacgtgg cagggcatgg cgatcatcag ggcgaaggcg agctcggcga cggccaggct
    15601 cgaggcctcg ggcgtgttgt cgacggcgat gcccttcgac ttcgcgtact cgacgtcgat
    15661 ggtgtcgacg ccgacgccgc cgcggatcac gtacttgagc ttcggcgcct tgtcgatcat
    15721 ggccttgtcg accttggtct tggagcggac gacgatgacc tcggcctcgg cgacccgggc
    15781 gacgtcggcg gtgacctcgc cgaagggctt gagcctcgcg ggcaggtccg gcgcgaaggc
    15841 gtcggccaga agtatgacca tgtgatcctc cctatgaaaa tggtgcgtcg ataaactgaa
    15901 tacagaatac aagattattc tagcacagct tcccggcctg tcaatatcgg cccgaaagcc
    15961 gccattttta ccgacaaaac gccgtttcgc attctacacc cgattctggc tctggggaag
    16021 caggaaaatg gaagccttgc cccgtgtcgg accagtgctg gacaggcgtc gggccggcgc
    16081 cggacagccg cgaacccgcg cctcattcca tcacagcccc tgcccgcgag ccggccctcg
    16141 gcaagccggc aagccggcgc gactaaagat agatctcccg cgcgaggggc tcgagccgct
    16201 cgcgggagag cacacggatc cggccgcgcc ggtcctccag gatgccctcc tcccggaagc
    16261 ggcggaccac gcgggatagc tggcggtagc tcgtcccgag gctgtccgcg agctcggaaa
    16321 ggtcgtcggt gccgtactcg gccgtgcctg actccgccgc gcccggcccg gcggcgccgg
    16381 cccgctcctc gagctccagg acggcgagga ggtagctcgc caggcggttc tccacgggat
    16441 agcggaggtt gatggcgctc tgcacgttga aggtcgcgag cttccggccg agccgcccgc
    16501 agaggaaggc gaagagacgg ccgttgcggt cggcggcctt ccgcgccgcc tcggccggca
    16561 aggacaggca ggccgtcgcc gtgatcgcct ccacgttgag cgtccaccgg gcataggcga
    16621 agagctcggc gtcgccgagc acctcgaagg gctcgtagaa gctcgcgagg agggatttcc
    16681 cgttctcgag gaggctgtag accttggccc tgccctcgac gaagaagagg agtcggtcgg
    16741 cggcctcgcc cgcccggagg atgagcccgc ccggctcggt ggcgacaagg ctcaaggcgc
    16801 caaggagatc ggcggagagg agctcggcta ggccgtagcg ctcgacgtag gcttcgaggg
    16861 attccatagc ccgcctccga aaaaacgcca tttaggacat acgtcctatt ccgaagtgta
    16921 gcgccggggg atactggcgg ccaagactgt acggagggca catggcctat ttcatcgcgg
    16981 ctcccttcct gggcgccttc atcaccctca tgaaccggct caacagcgac ctggccgccg
    17041 tcgtggggac ctacgcctcg agcctcgtca tccatctcgc cggcctcggc gccgtgtccc
    17101 tcgtcctcct ccgccggccc gagccgcgcc cggcccgccg ccctcccctg ctccgctact
    17161 cgggcggact cgtcggcctg gcgaccgtct tcctgtgcaa cgccgtctac ggcgccctcg
    17221 acgcctccct cgcggtcgcc ctcgccctcc tcgggcagat gctcggctcg atcctcgtcg
    17281 acgcgacggg cttcctgggg cggacgaagc gccccttcac ggcgcggagc ctgccgggcc
    17341 tcgccctggc cctggccggc gccgccgcgc tctcgggcgg cggacgcgcc gcgccaggcc
    17401 tcatcttcct cgccgtcgcc gccggcgccc tccccctcgt ctccatcacg ctgaacgcga
    17461 ggcttgccca ggacatcggc ctcttccgcg gcgtcagggc gaactacctt gtcgggctgg
    17521 cggcgaccct cctcctcgcg cccctcttcg gccgcgcggg ccttgccggg ctcgcggcgg
    17581 cggggagcgc caagcccctc cagctcgcgg ggggaggcat cctcggcgtg gccctggtcg
    17641 cgggcaccaa cctggtcttt ccccgcctgc ccgccctgca ggcctcgatc ctcatgtttt
    17701 ccggccagct cctctccggc ctcgccgtcg acgccgcctc gggcgccgcc atccaggcgc
    17761 ggacgcccct cggcctcctc gccgtcctcg cggggctggc cctggacgcc gggctcaaag
    17821 ccctcccgac ccggacttga ggaggcggtt gccgtaccag tcgccggcgt agacgccggc
    17881 ttaggcgcgg ccctacagga tcgagcccgg cctgaggctg gcggcgttca gcgcgccgag
    17941 ccgcgtccct tccgcgacgg cggccgccag ggagccgccc tcggcgagga cggccgcgag
    18001 gcccgccgtg aaggcgtcgc ccgagcccgt cgggttgagg gcctgcacgg ggcggacggc
    18061 ctcctcgcgg agggagcggc cgtcccacca ccaggtcggc tcgtgtccgc gcgtgaggac
    18121 gaggctcgtg ccgaagcgcc catggagctc gctcgccacc gccgccgcgt gggcgcggag
    18181 cccggccgcg tcgtccgcgg gagcggccgc ctcgccctcc gccgcggcgc cagccccact
    18241 cggcgcggcc ccgtgcaggg cacccgcgcc ggcccgtccg gggagcggcc aggtggccag
    18301 gaactccgag aggttcggct tcgcgaccgc cggccggtgc tccagggcgc ggaggaggtc
    18361 agggcccttc acgtcgagga cgaggaggag tccggcggcg cggacgcgtc gcgcgatctc
    18421 gggcaccagg tcttcctcga ggcccgccgc cttcgtcccc gagacgacga cggcccggca
    18481 ggcgggaagg agcctgtcga gctcgccaag gaggcggccg cccgtgccct cggccacagg
    18541 ctcggcctcc tccacgagct ccgtcgtccg gagcgctccg cgctcgacga gggtgtggca
    18601 gaagcggatc tccgagcccg actccacggc gcggaggtcc aggccgtccc tcgcgcagag
    18661 cgaaaggaac cagtccctgt tcgcgccgcc cgcctgcgag aggaggacgg ccggcctccc
    18721 cgtctggccg aggacgcggg cgacatcgac ggccttgccc gccgcgtcga cgcggtgctc
    18781 cccgctgcgg ttcacctcgc ccgggagcag gcggtcgaag aggagggtct tctggatgac
    18841 gggattgagg cagacgcaga ggatgggggc gggaggcggg ctcatggggc gactatagcc
    18901 gcggcgcgga gcagccgtcc accgagcggc gggcgtccgg cggccgcgac gccgcgcgcg
    18961 gccgcctgag accgaacggc ggccctccgt aaaaccggac agacttacga agacagtaac
    19021 tgaatgcccg cctaattaca taggcgaacg taatgccagg aagcggaggc tgcccaagga
    19081 tttcgtcctc gggcagcctc gtcagcatgt gccctgttac ggcgtccaat ccgagtagat
    19141 cgaacgaaat gcggtattat tccgccaagc gctggcagga aagccggaga taaaactaga
    19201 gagattggct tgacagtgct taattgcgga cgaattgcta ggatcaggga taaagccgat
    19261 caaatcgcca tctgatgttt tcatggttcc attctcaaag tcgacaggat cccaaccatc
    19321 tccaccatta tcataatcca tgtcgggcat gtacaattgg cctagatatg gttcaagtcc
    19381 attgtaaacg acatttttcg tcacggttcc gaccgaattg atggccttat agccaaccat
    19441 cggagctccg ttgtccagct tcaggctctt gagttcttca tgggccgcga ccacggcctc
    19501 tcggattggc cttaacgtgg catcgggaaa cgggacacca gcaaccgtca tgccagcgaa
    19561 atgcgtttta tcagcaagcg ctatctcatc taaggtagca tcgctaaaag tcgccccagc
    19621 gtagccgaca atccattctc cggcatcgcc ggcaccgctg tacagctccc ccgagtcgac
    19681 ccgctggaac tcatatgggt cacctgggtt caccaccatg ccgaggaagg cggtggc
//
LOCUS       DAOOSJ010000015        18779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2036_length_18779_cov_6.905950, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000015 DAOOSJ010000000
VERSION     DAOOSJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 18779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18779
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_18779_cov_6.905950"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLG14_01705"
     CDS             <1..699
                     /locus_tag="PLG14_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX47872.1"
                     /translation="ASILGNGSSELIGQLSVTVTVYLFNSVLMRRIGVAGVAAYTVAG
                     YLSFVESMLVLGCVQALVPLAGRSAGAGDRAASRAWLGVALRLALAVAAAAAAVALAA
                     PGAMARLFSKGGEAAAPGVVALAREAIVFTALAFIPGAFSMIAASYLTALGDAKGSAL
                     IASLRGLVLLSAFVLVLPLFLGDSGVWMAVPATELATFAVSVLLLRKSLRGASAALPA
                     AHGAAAVEGVPGQA"
     gene            880..1719
                     /locus_tag="PLG14_01710"
     CDS             880..1719
                     /locus_tag="PLG14_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47873.1"
                     /translation="MPSRKARPSSGKADGGPILGSASPGPAAEDTVPEFVAERSGINL
                     YSEGSLHAELKRRLARPGDRLEALVAGKVVDLVKADGELVEVQTKGLGKIVPKVLALA
                     AAGHRVRVVHPVALETRIKRIDPSSGELVSERRSPKRGDLYSLFDELVKATSLIAAPG
                     VTVEALLVRDSVLRVRDGTGSWRRKGDRTADRLLEEILDSRRLATRRDWLALIPRGLD
                     APYSSASLGEALGIAPDRARKILYSLARAGLLRETGRAGRRKTYEKAPAKRAARARRA
                     AKA"
     gene            1754..2350
                     /locus_tag="PLG14_01715"
     CDS             1754..2350
                     /locus_tag="PLG14_01715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47874.1"
                     /translation="MDRDSFRVEAAREADLPAVMRLEELGFIEAIREDEAVFRAKLAL
                     FPEGFVVLRESSTGLARGYLCAERWSEAPKPEEGPEALAAAFRLGHDPAERHDPSGRV
                     AYLASMTLDPALRGTGLGALLFDGGRRLILRRARGIERELLLVNEAWAAARRIYARSG
                     FVEGARLAGFFPGGKGEAPQGAILMEWKAVFSDRNAST"
     gene            2582..3496
                     /locus_tag="PLG14_01720"
     CDS             2582..3496
                     /locus_tag="PLG14_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HOX47875.1"
                     /translation="MFLFESIPWYSALMWFAVLGGLMLLNELARSSRWMSRLLFIAMP
                     ILLTVGVWPRTAGPGTSVGTWFHWAKVYSALAGSLGFMALRYYKKLAANKYFLMFPPF
                     ILALNILEAVVREFQVAGMNGVYDGMLIVGGPWNVMNAVSGLLNIVTICGWTGIFIGK
                     DEKKDMLWPDMLWFWVVAYDLWNFAYVYNCVPDHAFYAGAALLISCTIPAFFIKKGAW
                     LQHRAQTLAFWMMFVMVFPGFVDTSRFAVKSSHSVAALWVVSSLALASNVAVFVYQAW
                     KVLKLRKNPLRDELHTDLPEYQAVAAHR"
     gene            3646..4914
                     /locus_tag="PLG14_01725"
     CDS             3646..4914
                     /locus_tag="PLG14_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX47876.1"
                     /translation="MHLYERIVEDFRRSIEEGEIRPGERLPSIREAAERYGCNKLTAQ
                     RAFDLLAEGGFVENRPGSGSYVRFPSPPEERPGDFSAARLSEDFFPYEEAGRLFGEVL
                     AVERGKVFSASGPRGEPRLVEALARRYALPPDSLLLISGGQQGLDLCRRLFSAKEAPE
                     VLVEEPTYPGAISLFRPRASLPFGDEGPDPEAFERHWAERKGGGTALYYAVPDLHNPT
                     GLRYSLERKRAIAEAARRREVFIIEDDYISELEPSGLPRFVDLAPERTVWIKSLSKAA
                     APGLRIGLVAAPPRLLEELLRLRAETDTGPAAWLQLFAERLLASGLLDRHQAACADIA
                     RRRRAELLRLLSRFPGLSVPAASRGYNLWVSAPREARLAQAPWAEGARFGLSGSCRSR
                     FRLSLMSVGEAEWPQALARLERALAAAFGD"
     gene            5072..6007
                     /locus_tag="PLG14_01730"
     CDS             5072..6007
                     /locus_tag="PLG14_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX47877.1"
                     /translation="MKRYVNYLAGVGFASIFGFSFLVTKDALLAFSPFELLFLRFALA
                     TALMGALALLRLVRLDYRGKDPGPLVLACLFQPILYFASETFGVAKSATSTAGLVLGA
                     LPAAVAALGALMLKERLTRLQAASLCLSVAGVGLIVLGGGAAQAGEGSLFGLLFLFGA
                     LASAAFFNIYSRRASRVYSPVELTFAMMATGALSFGALALAESLLGGGAAGAAGAAGA
                     RAGLLSRATPGAWGAVLYLGALSSVVAFFLVNFSLSRVKASQSAVFSNLTTVISVAAG
                     VAFRGEAFGLPELLGSVLIVLGVFGTNAFGEKALH"
     gene            6037..7281
                     /locus_tag="PLG14_01735"
     CDS             6037..7281
                     /locus_tag="PLG14_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX47878.1"
                     /translation="MAEAAFYDIHCHALTLSHPSFLAFVETVRSRRMETLYAQARSPG
                     FLAGSLFLKGGERVRNMLSVMENDVGSIFALMEDDLLGLYAKPGDPPPLASEGGLELG
                     GLRFGRVVLAPLLMDFERRGGARTEAYYDRPPEKPMAAQVRDVLAGIRDYRRARPRGL
                     LEIRPFLGIDTSNYTEAALQALLESSFRGYRRGKAASRAAFEAMADIDVDRADDFPMR
                     FAGVKVYPPLGFDPWPDGGPGRDKAGLLYSFCESRDIPLTAHCDDQGFRVIPLDEAWL
                     YTAPSRWRPALEAHPGLRLDLAHFGHQYLRPLGRPQPGEWAEGILGLLGDFARVYTDF
                     SFNGCEPEYYRWLLELLGRQDPGLRSVALDRVMFGSDFPVNLTKVRSYADYCRLFADS
                     PLPEDWKRRFCSDNPERFLTGD"
     gene            complement(7378..8562)
                     /locus_tag="PLG14_01740"
     CDS             complement(7378..8562)
                     /locus_tag="PLG14_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX47879.1"
                     /translation="MPFFEPFRLERFFAAHEFSARLLLCTSDCESMPVRELLALEPGA
                     EEALLSLRLGYTETRGDPGLRSDIAKLYPGAGPSGLGPEEVFVDAGAEEAILNLCLAL
                     LSPGDHVVVNYPCYQSLAEIPRALGCAVSPWSLREEGGRWTLDPDELGRLLKAKTRLV
                     VLNMPHNPTGALMDRDTFGRSVELCRQAGAVLLVDEVYRHLERDPARRLPAACEAYEN
                     GVSLNVLSKSAGLAGLRIGWLASRRLDILDAVAVVKDYNSICSSGPSEVLAGVAVRNL
                     GRIAERNRALCASNLALLKGFFARRPGFAAWSEPEGGSIAFPRLGPDAAAAFPAAAGS
                     GGRGGDAEALALRLLSEAGVLLLPGAYYGFDPAYFRLGYGRASMPAALAALEAWLDSR
                     EE"
     gene            8821..10116
                     /locus_tag="PLG14_01745"
     CDS             8821..10116
                     /locus_tag="PLG14_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOX47880.1"
                     /translation="MKTPYRFRAIGSLPEDSPLVDAVVLVLPLVLLAFGLALARFDLR
                     EVAAGLGSIVRDPDILINDYVARAGLGTPFVNAGLCSLAAVLILKRSGVVISGPAMAA
                     VFTIAGFSLFGKNVYNIWPVMGGVWLYSRASRRPFSEYVIVALFGTALAPLASEVSFG
                     LGIPQPYNCLAGVAAGLASGFLIPPLAKHLLQFHQGYNLYNIGLTAGMVGTVAMSIFC
                     AFKVGGSGGGAWSQGNLGVYLPFVLALFGSLVAAGLAIGPRSRAGLRGILRSTGRLVS
                     DFPRSYGLGPSLVNMGLVGLIGAAYILLVGGDWNGPTVGALLTMCGFGAFGKHPRNVL
                     PVMAGVGLASLLTVYSPSAEVPLLAALFGTCLAPVAGRYGILAGMLAGALHLIVVVNV
                     GYLHGGLNLYNNGFSGGLVAGILVPVFEAIAERRSERGR"
     gene            10106..10537
                     /locus_tag="PLG14_01750"
     CDS             10106..10537
                     /locus_tag="PLG14_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47881.1"
                     /translation="MDAETVQLMRVAEELLKYLYLEGAREVELRYEFGEARSSCLAEA
                     KDLVLDDESLAELRRALKGPRQPELSEYYGGLVGKRIDASELALASTMAEAELIVSEP
                     VGGTRILVSRRRAAGPARGQAPRTARKPWFARGKPGGRSKD"
     gene            complement(10643..11413)
                     /locus_tag="PLG14_01755"
     CDS             complement(10643..11413)
                     /locus_tag="PLG14_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOX47882.1"
                     /translation="MSFDERTRIILGDGGMAALKAASAVVYGLGGVGAACAMDLVRAG
                     VGRIVALDFDELSESNLNRLYFGYREDVGRPKAEVFADYARRVNPEVLVEASPRFFSG
                     ASAEEAVAAAAAGGRAVHLDCVDSLNPKVNLLAALARSGLPFAASMGTAGRLDPGRLR
                     LTSFWLSQGCPLAREVRNRLRRLGVDADFPAVWSDEPPAPPVERPAGPAEEQKAPGRV
                     RKMQGSAPFVPQAAGHFLASWAVRRILEEAASGAPGSA"
     gene            complement(11410..12225)
                     /locus_tag="PLG14_01760"
     CDS             complement(11410..12225)
                     /locus_tag="PLG14_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX47883.1"
                     /translation="MLTDAHIHFADLAARDPGFAAAYAAGPYLACAASHDEPEFLATE
                     ALASPGPGRPKGLSFVSSFGIHPQWAVWKNADFLAGLAATGRIAAVGEAGFDFFGDRP
                     ERVRTKENLEAQTAVFEYQLELALARGLPMLLHVRKAMDLVFAYAPRLRRLPSLVLHS
                     FSGTAGEALALLERGLPAYFSFGAAVLNGHKRAEAACAAVPPDRILSETDAPWQPPRG
                     EAFCRLEHLDLVVGGMAALRGEDRAELEERLARNFAAAYPAAAGLVRARGGNT"
     gene            complement(12274..13581)
                     /locus_tag="PLG14_01765"
     CDS             complement(12274..13581)
                     /locus_tag="PLG14_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOX47884.1"
                     /translation="MDWLGTYKGKLVSVDEAVSRIESGNDVVVGQCASEPQGCMSRFH
                     TVADRVRDVRVFSVLTLKPYDFYMKPEMKGRFELASWFHAPGSRSALKAGTGTVTFVP
                     NMLHRAATDRIHARRPDIFFGTCTPPDKHGFVSLSLGVTYEKDIMEAARTVVLEVNPR
                     LPRTFGDTQVHVSQVDFFVEHDQEVPTLPAPDPSETDLAIGAHIAELVEDGSTIQLGI
                     GGIPNAAALALRDKKDLGVHTEMLVDSMMELYEMGVVTNARKSLKKGKFVTTFAMGSR
                     KFYDWLDDNVAVEFQRGRWVNDPAVIAQNSRMVSINTCISVDFTGQVASESIGPAQYS
                     GTGGQSDTATGAVAGTDGLGKSVIACYSTARNGSVSTIVPMLPEGSAVTLHRSLVDHV
                     VTEHGIAKLRGRTVSERTRALIGVAHPDFRKDLEAQARKLGYI"
     gene            complement(13593..14609)
                     /locus_tag="PLG14_01770"
     CDS             complement(13593..14609)
                     /locus_tag="PLG14_01770"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOX47885.1"
                     /translation="MARALIRGTGSYAPGEPIGNEELKRLAGVDFDTEKVVAKIGIKE
                     RHVARLRGLQESAADFAERAARTALADAGVGAEEVGLFIVATDTPEYVSPATGILLQG
                     RLQGGEREAEALDVGASCASFVTALDLAARRLATDPTLRYGLVAGVYNMPAHLRPGDA
                     FGWSIFADGAGAVLLERVEDGKAKGRGYLDGCFRADGTQWDYVGVYSGGAKKPVTREL
                     LERDEYGLELLQRLPGDRNVKLWPPLVRRLLEKARFPQDRVAHYFFTQINRSVIEEVM
                     GILGEPMAKTTTIMDRYGYTGSACVPMALHEARKAGRVGKGDAVVLVASGAGFAVGAD
                     LITL"
     gene            complement(14653..15810)
                     /locus_tag="PLG14_01775"
     CDS             complement(14653..15810)
                     /locus_tag="PLG14_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX47886.1"
                     /translation="MASAARSGKDTEERLIEAAVRLFARKWYGTVSVAEICREAGLSN
                     GIFYRYFENKETLFKRILESVTVEIEAAVAAAGGGDPRSRLRAFVLALVAFSEERKDL
                     VSVYREGQYRFFEYERRLVDIYLRGLSAALGREAGLPEYLFALGGVRFCAIRRALHGT
                     PADMDAIFSILKEGIFPGEGFDADRVFGGTVTPLPIDMEEGARDHLLRAGKRLFGEKG
                     FFETNIHEITDAAGLSVGAFYTHFESKDAFYAELIGLVGREVRGFIARNMGEGLTRLE
                     RELRGLWLFLVYLAIDRNCYNVVREAEFVLPEAVREYYAAFVRGYRRNPEGNGPVAAR
                     GEAAEETVIEFLLGIAHYFGIEVAFDRSPANARQAVEAIGRLLAGGFGAWL"
     gene            16041..17111
                     /locus_tag="PLG14_01780"
     CDS             16041..17111
                     /locus_tag="PLG14_01780"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOX47887.1"
                     /translation="MIHYLPEGEMHVGIIGIGTYLPPNYLTAADLSAETGVPEAVIAE
                     KFGIRRKPVAGPEDTTAEMGYKAALRALAQAGVEGRELDLVVWCGAQHKDYPCWLAGL
                     NVAQRLGAERAWSFDMEAMCGSMMAALDVAKSLMLVRGDLRTVLLVSGYRNNDLIDLR
                     EPATRFMMDIGSGGSACVLRKGAGRNVLLSSAFRGDGSLSEMCVVPVLGSKAWPPKAA
                     DAERPYFAVPDEAAFKARLGEVTMPNFYAVIRESLRLSGFHDSPPERAIDYLAILHFK
                     RSAHEAVLAELGLDGSKTSYLDDYGHIGQNDQLLSIELGLRDGKIRDGSRVVLVGAGL
                     GFVWAASVVRWGEFEEEGALHG"
     gene            17104..17844
                     /locus_tag="PLG14_01785"
     CDS             17104..17844
                     /locus_tag="PLG14_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX47888.1"
                     /translation="MDRMKGKVCVVTGGARGIGRGIAELFAREGAARVWALDVNAADF
                     PAIEAVSPAIKGIVVDVTNAAAAAAFVERVRNESGRIDVLVNNAGITRDALIQKMGDA
                     DWDAVINVNLKGVFIMTRAVAPVMMEGGSGAIVSISSIVGLDGNIGQTNYAATKGAVV
                     SMTKGWAKEFARKGAKVRANCVSPGYTRTPMIATVPEKVLEPFIAKTPLGRLAEIEDI
                     ANAVLFLSCDESAFVTGQTLRVDCGLVL"
     gene            17972..>18779
                     /locus_tag="PLG14_01790"
     CDS             17972..>18779
                     /locus_tag="PLG14_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX47889.1"
                     /translation="MKKAIAIVAALLTAASAFGQGTIKIGGIWPLSDITGKQGSMAAQ
                     LAVAEINAAGGVLGRQLELTVVDDELKADKAAAAVEKLVTVDNVEVLVGGMGSGAELG
                     KIPAFKKYGKVVMSTGAASYTVENALGADANWYFHLHPWDYNQGASYAEGWDAIQAAY
                     KNVKIKKIFLAYEEGAFGKASWDATKKLFGESGRYVIDGAPFKSAALGGGDYAAVLEA
                     AKDFKPDLYLWAGYDADALPMLEQAKAMKFSPGIYLGAPPGWPIGFGTSKL"
BASE COUNT         2584 a         6825 c         6508 g         2862 t
ORIGIN      
        1 gcctccatcc tcgggaacgg ctcctcggag ctcatcggcc agctctccgt cacggtcacg
       61 gtgtacctct tcaactcggt cctcatgcgg aggatcggcg tcgcgggcgt cgcggcctac
      121 acggtcgcgg gctacctctc cttcgtcgag tcgatgctcg tcctcggctg tgtccaggcc
      181 ctcgtgcccc tcgccgggcg gagcgccggc gcgggcgacc gggccgcctc gcgggcctgg
      241 ctcggcgtcg ccctgcgcct cgcgctcgcg gtcgccgccg ccgcggccgc cgtcgcgctc
      301 gccgcgccag gggcgatggc ccgcctcttc tcgaagggcg gcgaggcggc ggcgccgggc
      361 gtcgtggcgc tcgcccgcga ggcgatcgtc ttcacggccc tcgccttcat tccgggcgcc
      421 ttctccatga tcgccgcttc ctacctcacc gccctcggcg acgcgaaggg ttcggccctc
      481 atcgcgtcgc tgcgcggcct cgtcctgctc tccgccttcg tcctcgtcct tccgcttttc
      541 ctgggcgaca gcggcgtctg gatggccgtc ccggcgaccg agctcgcgac cttcgcggtc
      601 tcggtcctgc tcttgaggaa gagcctccgg ggggccagtg ccgccctgcc cgccgctcat
      661 ggggcggcgg ccgtggaggg cgtcccgggg caggcctagg cccggcgacg gacgggcgag
      721 acgggcgggc cgcgatagtg catggcgaga gtcaggcgcg aggggcgggc gcgatgcgcc
      781 cgccgcgccg gcgaagcgag gatccttcag tcgagcatgg gcagagtcgc atcgggccgc
      841 ggcttgcccc gcccggcggg caaggggtac agtgccctca tgccatcgag aaaagcccga
      901 ccatcttccg gcaaggccga cggaggcccg atccttggtt ccgcctcccc ggggccggcg
      961 gcggaggaca cggtccccga gttcgtagcc gagcgctcgg gcatcaacct ctactccgag
     1021 gggagcctgc acgccgagct caagcggcgc ctcgcccgtc ccggcgaccg cctcgaggcc
     1081 ttggtcgcgg gcaaggtcgt cgacctcgtc aaggccgacg gggagctcgt cgaggtgcag
     1141 acgaagggcc tcggcaagat agtccccaag gtcctggccc tcgcggccgc cggccatagg
     1201 gtgcgcgtcg tccaccccgt cgccctggag acccgcatca agcgcatcga tccctcgagc
     1261 ggcgagctcg tctccgagcg caggagcccg aagcgggggg atctctactc gctcttcgac
     1321 gagctcgtga aggcgacgag cctcatcgcc gcgccaggcg tcacggtcga ggccctcctc
     1381 gtgcgggact cggtgctgcg cgtccgcgac ggcacgggct cctggcgccg gaagggcgac
     1441 cgcaccgcgg accgcctcct cgaggagatc ctcgattccc ggcggctcgc gacgaggcgc
     1501 gactggctcg ccctcatccc gcgggggctg gacgcgccct attcctcggc gagcctcggc
     1561 gaggcgctcg gcatagcgcc cgaccgcgcg cggaagatcc tctactccct ggcccgcgcc
     1621 ggcctgctcc gcgagacggg gagggcggga cggcgcaaga cctacgagaa ggctcccgcc
     1681 aagcgggcgg cgcgtgccag gcgggcggcg aaggcttaat cagcggcgcg gcgcgtgcta
     1741 tagtccgcgt atcatggaca gggattcctt ccgcgtcgag gcggcccgcg aggccgacct
     1801 tcccgccgtc atgaggctcg aggagctggg cttcatcgag gcgatacgcg aggacgaggc
     1861 cgtcttcagg gccaagctcg ccctcttccc ggagggcttc gtcgtcctgc gcgagtcttc
     1921 gacgggcctg gcccgcggct acctctgcgc cgagcgctgg tccgaagcgc ccaagcccga
     1981 ggagggacct gaggccctcg cggcggcctt caggctgggc cacgatcccg ccgagcgcca
     2041 cgacccctcg ggccgggtcg cctatctcgc ctccatgacc ctcgacccgg ccctgcgcgg
     2101 aacggggctc ggcgccctcc tctttgacgg ggggcggcgc ctcatcctgc gccgcgcccg
     2161 cggcatcgag cgggagctcc ttctcgtgaa cgaggcctgg gccgccgcgc ggcgcatcta
     2221 cgcccgctcg ggcttcgtcg aaggcgcgcg cctcgcgggc ttcttccccg gcgggaaggg
     2281 cgaggctccc caaggggcca tccttatgga gtggaaagcc gtttttagtg acagaaacgc
     2341 ctcgacgtga cgctttagtc gataaagatt gagcgtatcc ttgacacgcc gtacagggcg
     2401 gcgtgatact gccaaaaccg gcgtcgccgg tcaggatacg cgattccggt ccgtaagtcc
     2461 gcaccgcccg cccgcgcctt taagcgacgc gcccgagccg cttccagcga acccagcgcc
     2521 tggtcgccgt tcccaccacc ccacgcgccg gagccccgtc ccgcgcacgc aaggtcccgc
     2581 catgttcctc ttcgagtcca tcccctggta ttccgccctc atgtggttcg ccgtcctggg
     2641 cggcctcatg ctcctcaacg agctcgcccg ctcgagcagg tggatgtccc gcctcctctt
     2701 catcgccatg cccatcctcc tcaccgtcgg cgtctggccc cgcaccgccg gccccggcac
     2761 gagcgtcggc acctggttcc actgggcgaa ggtctactcg gccctggccg gcagccttgg
     2821 cttcatggcc ctgcgctact acaagaagct cgccgcgaac aagtacttcc tgatgttccc
     2881 gcccttcatc ctggccctca acatcctgga ggccgtggtc agggaattcc aggtcgcggg
     2941 catgaacggc gtctacgacg gcatgctcat cgtcggcggc ccctggaacg tgatgaacgc
     3001 cgtctccggc ctcctcaaca tcgtcacgat ctgcggatgg acgggcatct tcatcggcaa
     3061 ggacgagaag aaggacatgc tctggcccga catgctctgg ttctgggtcg tcgcctacga
     3121 cctctggaac ttcgcctacg tctacaactg cgtccccgac cacgccttct acgccggcgc
     3181 cgccctcctc atctcctgca cgatcccggc cttcttcatc aagaagggcg cctggctcca
     3241 gcaccgcgcg cagaccctgg cgttctggat gatgttcgtg atggtcttcc ccggcttcgt
     3301 cgacacctcc cgcttcgcgg tgaagtcctc gcacagcgtc gccgccctct gggtggtctc
     3361 gagcctggcc ctggcctcca acgtcgcggt cttcgtctac caggcctgga aggtcctcaa
     3421 gctcaggaag aaccccttga gggacgagct ccacacggac ctgcccgagt accaggcggt
     3481 cgccgcccac aggtaggctc tcgccacagg taggcgccca aggccccccg tcggggcccg
     3541 cctagagccg gccgcccggg cctcccgggc ggccggcttt ttttcgccgc cgcgcggcgg
     3601 ggcgcggtgt ttccgcgcct gaccctccgt gctatgcttc ggcccatgca tctctacgag
     3661 cgcatcgtcg aggacttccg gcgttccatc gaggagggcg agatccgccc cggcgagcgc
     3721 ctcccctcga tccgcgaggc cgccgagcgc tacggctgca acaagctcac cgcccagcgc
     3781 gccttcgacc tcctggccga ggggggcttc gtcgagaacc ggcccggctc ggggagctac
     3841 gtccgattcc cctctccccc cgaggagcgg cccggcgact tctcggcggc gcggctctcc
     3901 gaggacttct ttccctacga ggaggcgggc cgcctcttcg gcgaggtcct ggccgtcgag
     3961 cgggggaagg tcttctccgc ctccggcccg cgcggcgagc cccgcctggt cgaggccctg
     4021 gcgcggcgct acgccctccc tcccgactcc ctcctcctca tctccggcgg ccagcagggc
     4081 ctcgacctct gccgccgcct tttctccgcg aaggaggcgc cggaggtcct cgtggaggag
     4141 ccgacctacc ccggcgccat ctccctcttc aggccccgcg cctcccttcc cttcggcgac
     4201 gagggccccg accccgaggc cttcgagcgc cactgggcgg agaggaaggg cgggggaacg
     4261 gccctctact acgcggtgcc cgacctccac aacccgacgg gactccgcta ctccctggag
     4321 cggaagcggg ccatagccga ggcggcgcgg cggcgcgagg tcttcatcat cgaggacgac
     4381 tacatctccg agctcgagcc ctcgggcctg ccgcgcttcg tggacctggc gccggagcgg
     4441 acggtctgga tcaagtccct ctccaaggcg gccgcccccg gcctccgcat cggcctcgtc
     4501 gccgcgccgc cgcgcctcct cgaggagctc ctccgcctcc gcgcggagac cgacaccggg
     4561 cccgcggcct ggctccagct cttcgccgag cgcctcctcg cctcgggcct cctcgatcgt
     4621 caccaggccg cctgcgccga catcgcccgg cgacgccgcg ccgagctcct ccgcctcctg
     4681 tcgcgcttcc ccggcctctc cgtgcccgcc gcctcgcgcg gctacaacct ctgggtctcc
     4741 gccccccgcg aggcccgcct ggcccaggcg ccctgggccg agggcgcccg cttcggcctt
     4801 tcgggctcct gccgctcccg cttcaggctc tccctcatgt ccgtcggcga ggccgagtgg
     4861 ccccaggccc tcgcgcggct cgagcgtgcc ctggcggcgg ccttcggcga ctgagtctgc
     4921 gcggctcctt gacgtccggg gccgccgccg gtagcctcga cgggccaggt cctcctcctc
     4981 gcgcaggccg aggcggccgc gatacccttc gcgggaccct gcggcccgcc tcgggccgca
     5041 gggagcggga cgcggcggga agggcggcgg catgaaacgt tatgtgaact acctcgcggg
     5101 agtcggcttc gcctcgatct tcggcttctc cttcctcgtc acgaaggacg cgctcctggc
     5161 cttcagcccc ttcgagctcc tcttcctccg cttcgccctg gcgacggccc tcatgggagc
     5221 gctggccctc ctccgcctgg tccggctcga ctaccggggc aaggacccgg ggcccctcgt
     5281 cctcgcctgc ctcttccagc ccatcctcta cttcgcctcc gagaccttcg gcgtcgcgaa
     5341 gtcggcgacg agcacggccg gcctcgtcct cggggccctg cccgcggccg tggccgccct
     5401 gggcgccctc atgctcaagg agcgcctcac gcggctccag gcggcgagcc tctgcctgtc
     5461 ggtcgcgggc gtgggcctca tcgtcctggg cggcggggct gcccaggcgg gggaggggag
     5521 cctcttcggc ctcctcttcc tcttcggcgc cctcgcgagc gccgccttct tcaacatcta
     5581 ttcccgccgg gcgagccgcg tctacagccc cgtcgagctg accttcgcga tgatggcgac
     5641 cggcgccctg tccttcggcg ccctcgccct ggccgaatcc ctcctgggcg gcggagccgc
     5701 gggggccgcc ggcgccgcgg gcgccagggc aggcctccta tcgcgcgcga cgccgggggc
     5761 ctggggagcc gtcctctacc tcggcgccct ctcctccgtc gtcgccttct tcctcgtgaa
     5821 cttctccctg tcgcgggtca aggcctcgca gtccgccgtc ttctcgaacc tcacgacggt
     5881 gatctccgtc gcggcgggag tggccttccg cggcgaggcc ttcggccttc ccgagctcct
     5941 gggctccgtc ctcatcgtcc tgggcgtctt cggcacgaac gccttcgggg agaaagcgct
     6001 acactagagg gagagcagca agccgggagg cgccgcatgg ccgaggccgc tttctacgac
     6061 atccactgcc acgccctcac cctctcccac ccgagcttcc tcgccttcgt ggagacggtg
     6121 cggagccgcc gcatggagac cctctacgcg caggcgcggt cgccgggctt cctcgcgggc
     6181 tccctcttcc tgaagggggg cgagcgggtg cggaacatgc tttcggtgat ggagaacgac
     6241 gtcggctcca tcttcgccct catggaggac gacctcctcg gcctctacgc caagccggga
     6301 gaccctccgc ccctcgcctc ggagggcggt ctcgagctgg gcggcctccg cttcggccgc
     6361 gtcgtcctgg cccccctcct catggacttc gagcggcgcg gaggcgcgag gacggaggcc
     6421 tactacgaca ggccgcccga gaagcccatg gcggcccagg tccgcgacgt gctggccggg
     6481 atcagggact accggcgggc ccgtccccgc ggcctcctcg agatcaggcc cttcctcggg
     6541 atcgacacgt cgaactacac cgaggccgcg ctccaggccc tcctcgagtc ctccttccgg
     6601 ggctaccgcc gcgggaaggc ggcgtcccgg gcggccttcg aggcgatggc cgacatcgac
     6661 gtggaccgcg ccgacgactt ccccatgcgc ttcgcggggg tgaaggtcta cccgcccctc
     6721 ggcttcgatc cctggccgga cggaggcccc gggcgggaca aggccgggct tctctactcc
     6781 ttctgcgagt cgcgggacat accgctcacc gcgcactgcg acgaccaggg cttcagggtg
     6841 atacccctgg acgaggcctg gctctacacc gcgccgtccc gctggaggcc ggccctggag
     6901 gcccatcccg gcctgcgcct cgacctggcc cacttcggcc accagtacct ccgtccattg
     6961 gggcggcccc agccgggcga gtgggccgag ggcatactcg gcctccttgg cgatttcgcg
     7021 cgggtctaca cggacttctc cttcaacggc tgcgagcccg agtactaccg ctggcttctc
     7081 gagctcctgg gccgccagga tccggggctc cgctccgtcg ccctggaccg cgtcatgttc
     7141 ggcagcgact tccccgtgaa cctcacgaag gtccgctcct acgcggacta ctgccgcctc
     7201 ttcgccgact cgcccctgcc cgaggactgg aagcgccgct tctgcagtga caatccggag
     7261 cgcttcctca ccggggactg aggccactcc tggcgggccg cgcggggccc tggcgtggcc
     7321 ctggcgtggc ccggggcggc gcgttgtccg cgcgacgggc gccccggccg cgccgcctca
     7381 ctcctcgcgc gagtccagcc aggcctccag ggcggccagg gccgccggca tggaggcgcg
     7441 gccgtagcca agccggaagt aggcggggtc gaagccgtag taggcgccgg gaaggaggag
     7501 gacgcccgcc tccgaaagga ggcgcagggc gagggcctcg gcgtcgccgc cccttccgcc
     7561 cgaccccgcc gccgcgggaa aggccgcggc cgcgtcgggg cccagccggg ggaaggcgat
     7621 ggagcctccc tcgggctcgc tccaggccgc gaagccgggg cggcgcgcga agaagccctt
     7681 gagcagggcg aggttcgagg cgcagagggc gcggttccgc tcggcgatcc tcccgaggtt
     7741 ccgcacggca acgccggcca ggacctcgct cgggcccgag gagcagatcg agttgtagtc
     7801 cttcacgacg gcgacggcgt ccaggatgtc caggcggcgg gaggcgagcc agccgatccg
     7861 gaggccggcc aggcccgcgc tcttggagag cacgttgagc gacactccgt tctcgtaggc
     7921 ctcgcaggcg gccgggaggc ggcgcgcggg gtcccgctcc aggtggcggt agacctcgtc
     7981 cacgaggagg accgcgccgg cctgccggca gagctcgacc gagcggccga aggtgtcccg
     8041 gtccatgagc gcccccgtcg ggttgtgcgg catgttgaga acgacgagcc tcgtcttcgc
     8101 cttgaggagg cgcccgagct cgtcgggatc gagggtccag cggccgccct cctcgcggag
     8161 gctccagggg gagacggcgc agcccagggc cctcgggatc tccgcgaggg actggtagca
     8221 ggggtagttc acgacgacgt ggtcgccggg cgacaggagc gcgaggcaga ggttgaggat
     8281 cgcctcctcc gcgccggcgt caacgaagac ctcctcgggg ccgagtcccg agggccctgc
     8341 ccccgggtag agcttggcga tgtccgacct gaggcccggg tcgccgcgcg tctccgtgta
     8401 gccgagcctg agggagagga gagcctcctc ggctcccggc tccagggcca ggagctcgcg
     8461 gacgggcatg ctctcgcagt ccgaggtgca aaggaggagc cgcgcggaga actcgtgggc
     8521 ggcgaagaag cgctctaggc ggaagggctc gaagaagggc atcggtacct ccagggggat
     8581 gccgacatgg tagcgcctat ccggcccgcg gggaagggcg gatcgcccgg ggagcccggc
     8641 cggcggcgct ccggccggcg cctcgccgcg gagccccgtc gcggagcccc gccgtggagc
     8701 cccgtcgcga agccccgccg tggagcctca ggcgataccc ctcggcgatg tttcgccaag
     8761 tagaaaaaag tcctggccgg cgcttgcgcc gaggagccta gggcggcgat acttaggccc
     8821 gtgaaaaccc cctatcgatt ccgcgcgatc gggagtcttc ccgaggactc ccccctcgtc
     8881 gacgcggtcg tgctggtctt gcccttagtc ctcctcgcct tcggcctggc cctcgcccgc
     8941 ttcgacctgc gggaggtggc cgcgggcctg ggctccatcg tcagggaccc cgacatcctc
     9001 atcaacgact acgtcgcccg cgcgggcctc gggacgccct tcgtcaacgc ggggctctgc
     9061 tccctcgcgg ccgtcctcat cctaaagcgc tcgggcgtcg tcatctccgg gcccgccatg
     9121 gccgcggtct tcaccatcgc gggcttctcc ctcttcggga agaacgtcta caacatctgg
     9181 cccgtcatgg gcggggtctg gctctactcc cgcgcctcgc gccgcccctt ctccgaatac
     9241 gtcatcgtgg ccctcttcgg cacggccctg gctccgctcg cgagcgaggt gagcttcggc
     9301 ctcggcatcc cgcagcccta caactgcctc gccggcgtcg ccgcgggcct cgcctcgggc
     9361 ttcctcatcc ctcccctcgc caagcacctc ctccagttcc accagggcta caacctctac
     9421 aacatcggcc tgaccgccgg catggtcggc acggtggcca tgtcgatctt ctgcgccttc
     9481 aaggtgggcg gctcgggcgg cggcgcctgg tcgcagggga acctgggcgt ctacctgccc
     9541 ttcgtcctcg ccctcttcgg ctccctcgtc gccgccggcc tcgccatcgg accgcggtcg
     9601 cgcgccgggc tgcgtggcat cctcaggtcg acgggccgcc tggtgagcga cttcccgagg
     9661 agctacggcc tcggcccgag cctggtcaac atgggcctgg tcggcctcat cggggcggcc
     9721 tacatcctcc tcgtgggcgg ggactggaac gggccgaccg taggcgccct cctcacaatg
     9781 tgcggcttcg gggccttcgg gaagcatccc aggaacgtcc tccccgtgat ggccggcgtc
     9841 ggtctcgcct ccctcctcac ggtctattcg ccctccgcgg aggtgcccct tctcgccgcc
     9901 ctcttcggca cctgcctcgc ccccgtggcc gggcgctacg ggatcctggc cggaatgctg
     9961 gcgggagccc tccatctcat cgtcgtggtc aacgtaggct atctccacgg cgggctcaac
    10021 ctctacaaca acggcttctc gggaggcctc gtggccggca tcctcgtgcc ggtcttcgag
    10081 gcgatcgccg agaggaggag cgaacgtgga cgctgagacg gttcagctca tgcgggtggc
    10141 cgaggagctg ctcaagtacc tgtatctcga gggagcgcgg gaggtcgagc tccgctacga
    10201 gttcggggag gcgcgctcga gctgcctcgc cgaggccaag gacctcgtgc tcgacgacga
    10261 gagcctggcc gagctcaggc gggccctcaa ggggccgcgc cagcccgagc tttccgagta
    10321 ctacggcggc ctcgtgggga agcgcatcga cgcctccgag ctcgccctgg cctcgaccat
    10381 ggcggaggcc gagctcatcg tgtcggagcc cgtcggcggc acgcgcatcc tcgtgagccg
    10441 ccgaagggcg gccggcccgg cgcgcggaca ggcgccgcgc acggcgcgga agccctggtt
    10501 cgcgcgcgga aagccgggcg gccggtccaa ggactgaggc tgcccgatcc ccgtggccgg
    10561 ctacgccccg gcgcggccgt cgcttggcgc ggcccgcgcc gggtcgacgc gccccttcgc
    10621 gtggccggcc cggctatgcc gcctaagcgg agcccggagc gccggaggcc gcctcctcga
    10681 ggatgcggcg cacggcccag ctcgcgagga agtgccccgc ggcctgcggc acgaagggcg
    10741 ccgaaccctg catcttcctc acccgccccg gcgccttctg ctcctccgcg gggccggcgg
    10801 gccgctcgac gggaggcgcg ggcggctcgt cgctccagac cgcggggaag tccgcgtcca
    10861 cgcccaggcg gcgcagccgg ttccgcacct cgcgcgccag ggggcagcct tgcgacagcc
    10921 agaaggaagt gaggcggagc cgccccgggt cgagcctgcc ggccgtgccc atgctcgccg
    10981 cgaagggaag gcccgagcgg gccagggcgg ctaggaggtt gaccttggga ttgagggagt
    11041 cgacgcagtc caggtggacg gcccgcccgc ccgcggcggc cgcggcgacc gcctcctccg
    11101 ccgaggcgcc ggagaagaag cggggggagg cctcgacgag gacctcggga ttgacccggc
    11161 gggcgtagtc ggcgaaaacc tcggccttgg gccttcccac gtcttcgcga tagccgaaat
    11221 agagcctgtt caggttggac tcggagagtt cgtcgaagtc cagggcgacg atccgcccga
    11281 cccccgcgcg cacgaggtcc atggcgcagg ccgctcccac gccgcccagg ccgtagacga
    11341 cggccgacgc ggccttgagc gccgccattc ccccgtcgcc gaggatgatc ctcgtcctct
    11401 cgtcgaagct caagtattcc ctcctctcgc ccggacgagg ccggccgcgg cgggataggc
    11461 cgcggcgaag ttccgggcga gccgctcctc gagctccgcg cggtcctcgc cgcggagcgc
    11521 ggccatcccg ccgaccacga ggtcgaggtg ctcaaggcgg cagaaggcct cgccgcgggg
    11581 cggctgccag ggcgcgtcgg tctcggagag gatcctgtcc ggaggcacgg cggcgcaggc
    11641 ggcctccgcg cgcttgtggc cgttcaggac cgccgcgccg aaggagaagt aggccgggag
    11701 cccccgctcg aggagggcaa gggcctcgcc ggccgtgccg gaaaaggagt ggaggacgag
    11761 ggagggcagg cgcctgaggc gcggcgcgta ggcgaagacg aggtccatcg ccttcctgac
    11821 gtggagtagc atcgggagcc cccgcgcgag ggcgagctcg agctggtact cgaagacggc
    11881 cgtctgggcc tcgaggttct ccttggtccg gacgcgctcg ggccggtcgc cgaagaagtc
    11941 gaagccggcc tcgccgaccg cggctatgcg gccggtggcg gcgagccccg cgaggaaatc
    12001 cgcgttcttc cacacggccc actgggggtg gatgccgaag ctcgagacga aggagaggcc
    12061 cttgggccgg cccggtcctg gcgaggcgag ggcctccgtc gcgaggaatt cgggctcgtc
    12121 gtggctcgcg gcgcaggcga gataggggcc ggccgcgtag gccgcggcga agcccgggtc
    12181 ccgcgcggcg aggtcggcga agtggatgtg ggcgtcggtg agcacggcgc caggatagcg
    12241 cgcgccgggc ggcgccgcaa ccgggcgtcc cgcctagatg tagccgagtt tccgcgcctg
    12301 cgcctcgagg tccttgcgga agtcgggatg ggcgacgccg atcagggccc tggtccgctc
    12361 gctcacggtc cggccgcgga gcttcgcgat gccgtgctcg gtgacgacgt ggtcgacaag
    12421 gctccggtgc agggtgaccg ccgatccctc cggcagcatg ggcacgatgg tggagacgct
    12481 cccgttcctg gccgtcgagt agcaggcgat gacgctcttc cccaggccgt cggtccccgc
    12541 cacggcgccc gtggccgtgt cgctctggcc gcccgtgccg gagtactggg cggggcctat
    12601 gctctcgctc gcgacctggc ccgtgaagtc gaccgagatg caggtgttga tggagaccat
    12661 gcgcgagttc tgcgcgatga cggcgggatc gttgacccag cggccccgct ggaattcgac
    12721 ggcgacgttg tcgtcgagcc agtcgtagaa cttcctggag cccatcgcga aggtggtgac
    12781 gaacttgccc ttcttgaggc tcttccgggc gttcgtgacg acgcccatct cgtagagctc
    12841 catcatggag tcgacgagca tctcggtgtg cacgcccagg tccttcttgt cccggagggc
    12901 gagggccgcc gcgttgggga tgcccccgat gccgagctgg atggtggagc cgtcctccac
    12961 gagctccgcg atgtgggcgc cgatcgcgag gtcggtctcg ctggggtcgg gggcggggag
    13021 ggtaggcacc tcctggtcgt gttccacgaa gaagtcgacc tgggagacgt ggacctgggt
    13081 gtcgccgaag gtgcggggca ggcggggatt cacctcgagg acgacggtcc gcgcggcctc
    13141 catgatgtcc ttctcgtagg tgacgcccag ggagagggag acgaagccgt gcttgtcggg
    13201 aggggtgcag gtgccgaaga agatgtcggg ccgcctggcg tggatgcggt ccgtggccgc
    13261 gcggtggagc atgttcggca cgaaggtcac ggtgcccgtg cccgccttca gggccgagcg
    13321 cgagccgggc gcgtggaacc aggacgcgag ctcgaagcgg cccttcatct cgggcttcat
    13381 gtagaagtcg tagggcttga gcgtcaggac ggagaagacc ctgacgtccc gcacccggtc
    13441 ggcgacagtg tggaagcgcg acatgcagcc ctggggctcc gaggcgcact ggccgacgac
    13501 gacgtcattc ccgctctcga tcctggagac ggcctcgtcg acggagacga gttttccctt
    13561 gtaggtgcca agccaatcca tcctgaagct ccttacagcg ttatgaggtc ggcgccgacc
    13621 gcgaagccgg ctccgctcgc cacgaggacg accgcgtcgc ccttcccgac ccggcccgcc
    13681 ttccgcgcct cgtgcagggc catcgggacg caggccgagc ccgtgtagcc gtagcggtcc
    13741 atgatcgtgg tggtcttcgc catgggctcg ccgaggatgc ccatgacctc ctcgatgacc
    13801 gaacggttga tctgcgtgaa gaagtagtgc gccacccggt cctgggggaa gcgcgccttc
    13861 tcgaggaggc gcctcaccag gggcggccag agcttcacgt tcctgtcgcc cggaaggcgc
    13921 tggaggagct cgagtccgta ctcgtcccgc tcgaggagct cgcgcgtgac gggcttcttc
    13981 gcgccgcccg agtagacgcc cacgtagtcc cactgcgtgc cgtcggcgcg gaagcagcca
    14041 tccaggtagc cgcggccctt ggccttcccg tcctcgaccc tctccagtag gacggcgccg
    14101 gcgccgtcgg cgaagatgga ccagccgaag gcgtcgccgg ggcggagatg ggcgggcatg
    14161 ttgtagacgc ccgccacgag gccgtagcgc agggttgggt cggtggccag gcgcctggcg
    14221 gccaggtcga gggcggtcac gaagctcgcg caggaggccc cgacgtcgag ggcctcggcc
    14281 tcgcgctcgc cgccctggag ccggccctgg aggaggatgc ccgtcgccgg ggagacgtac
    14341 tcgggcgtgt cggtcgcgac gatgaagagg cccacctcct ccgcgccgac gcccgcgtcg
    14401 gccagggccg tgcgggcggc ccgctcggcg aagtccgccg cgctctcttg gaggccgcgg
    14461 agcctcgcga catgtctttc cttgatgccg atcttcgcca cgaccttctc cgtgtcgaag
    14521 tcgacgcccg cgagccgctt gagctcctcg ttgcctatgg gctctccggg cgcgtaggag
    14581 cccgtccccc tgatgagcgc tcttgccatg cggtacctcc cgcgccgccc agccggcgct
    14641 cctgtcgatt tcctagagcc aggcgccgaa gcctcccgcg aggaggcgcc ctatcgcctc
    14701 gactgcctgc cgggcgttcg ccggagagcg atcgaaggcg acctcgatgc cgaaatagtg
    14761 ggcgatgccg aggaggaact cgatcaccgt ctcctcggcg gcctcgccgc gggccgccac
    14821 ggggccgttg ccctcggggt tccgccggta gccgcggacg aaggcggcgt agtactcgcg
    14881 caccgcctcg ggcagcacga actccgcctc gcggacgacg ttgtagcagt tgcggtcgat
    14941 ggcgaggtag acgaggaaga gccagaggcc gcgcagctct cgctccaggc gggtgaggcc
    15001 ctcgcccatg ttgcgggcga tgaagccgcg gacctcgcgg ccgacgaggc cgatgagctc
    15061 cgcgtagaag gcgtccttgg actcgaagtg ggtgtagaag gcgccgacgg agagtcccgc
    15121 ggcgtcggtg atctcgtgga tgttggtctc gaagaagccc ttctccccga agagccgctt
    15181 gcccgcccgg agcagatggt cccgcgcgcc ctcctccatg tcgatgggga ggggcgtcac
    15241 cgtgccgccg aagacgcggt cggcgtcgaa gccctcgccg gggaagatgc cctccttcag
    15301 gatggagaag atggcgtcca tgtcggccgg cgttccgtgg agggcgcggc ggatcgcgca
    15361 gaagcgcacg ccgcccaggg cgaagaggta ctcgggaagg cctgcctcgc ggcccagggc
    15421 cgcggagagg ccgcgcaggt agatgtcgac gaggcgccgc tcgtactcga agaagcggta
    15481 ctggccctcg cggtagaccg agaccaggtc cttcctctcc tcggagaagg cgaccagggc
    15541 gaggacgaag gcgcggagcc gggagcgggg atcgccgccc cccgcggcgg cgaccgccgc
    15601 ctcgatctcc accgtcaccg actcgaggat gcgcttgaag agggtctcct tgttctcgaa
    15661 gtagcggtag aagatgccgt tggagagtcc cgcctcgcgg cagatctcag cgaccgagac
    15721 ggtcccgtac cacttcctcg cgaacagccg tacggcggct tctatgagcc tctcctcggt
    15781 gtccttgccc gaccgcgccg cgctggccat gacagctcct tgcttgacga tgagagacga
    15841 ttcagtgctc tcgggctgag tatagcccga tttccgaacg ggagcaagaa aaacctttgg
    15901 ctctggggcg cattgtatgt ctattcgctt atttattgat aaaaatgcca aaaacgccat
    15961 ctggcggcgg cgggcagtct agaaatatca caaaatcgcg cttgaaggcc tccgtaaacc
    16021 gtggttatct cgaatgagag ttgattcatt atttacctga aggcgagatg cacgtcggca
    16081 tcatcggcat cggcacctat ctacctccga actacctgac cgcggccgac ttgtcggccg
    16141 agaccggcgt gcccgaggcg gtgatagccg agaagttcgg catccgccgc aagccggtcg
    16201 ccggccccga ggacacgacg gcggagatgg gctacaaggc ggccctgcgc gccctggccc
    16261 aggccggcgt cgagggtcgg gagctcgacc tggtggtctg gtgcggcgcc cagcacaagg
    16321 actatccctg ctggctcgcc ggcctcaacg tggcgcagcg cctgggcgcc gagcgcgcct
    16381 ggagcttcga catggaggcg atgtgcggct ccatgatggc cgccttggac gtggccaagt
    16441 ccctgatgct ggtccggggc gacctgcgca ccgtcctcct cgtctccggc taccgcaaca
    16501 acgacctcat cgacctcagg gagccggcga cccgcttcat gatggacata ggctcgggcg
    16561 gctcagcctg cgtcctccgg aagggcgcgg gccgcaacgt cctcctctcc tccgccttcc
    16621 ggggcgacgg ctccctctcc gagatgtgcg tggtgccggt cctcggctcc aaggcctggc
    16681 cgcccaaggc cgccgacgcg gagcggccct acttcgcggt cccggacgag gccgccttca
    16741 aggcgaggct gggcgaggtg accatgccca acttctacgc ggtgatccgc gagtccttgc
    16801 ggctgtccgg cttccacgac tcgccgccgg agcgcgccat cgactacctc gcgatactcc
    16861 acttcaagcg gagcgcgcac gaggccgtcc tggccgagct cggcctggac ggatcgaaga
    16921 ccagctatct cgacgactac ggccacatcg gccagaacga ccagctcctg tcgatcgagc
    16981 tcggcctccg cgacggcaag atccgcgacg gctcgcgcgt cgtgctcgtc ggcgcggggc
    17041 taggcttcgt gtgggcggcg agcgtggtcc gttggggcga attcgaagaa gaaggagcgc
    17101 tgcatggata ggatgaaggg aaaggtctgc gtcgtcacgg gcggcgcgcg cggcatcggc
    17161 cgcggcatcg cggagctctt cgcccgcgag ggcgcggccc gcgtctgggc cctggacgtc
    17221 aacgcggccg atttccccgc gatagaggcc gtctcgccgg ccatcaaggg catcgtggtg
    17281 gacgtgacga acgcggccgc cgccgcggcc ttcgtggagc gggtccggaa cgagtccggc
    17341 cggatcgacg tactcgtgaa caacgcgggc atcacccgcg acgcgctcat ccagaagatg
    17401 ggcgacgccg actgggacgc cgtcatcaac gtcaacctga agggcgtctt catcatgacc
    17461 cgggccgtcg cgcccgtcat gatggagggc ggctccgggg ccatcgtctc catctcgagc
    17521 atcgtcggcc tggacggcaa catcggccag accaactacg cggccacgaa gggcgcggtc
    17581 gtgtcgatga ccaagggctg ggccaaggaa ttcgcgcgga agggcgccaa ggtgcgcgcg
    17641 aactgcgtgt ccccgggcta cacgcgcacg ccgatgatcg cgacggtgcc ggagaaggtg
    17701 ctcgaaccct tcatcgccaa gacgccgctc gggcggctcg cggagatcga ggacatcgcc
    17761 aacgcggtgc tcttcctctc ctgcgacgag tccgccttcg tgacgggaca gaccctccgc
    17821 gtggactgcg ggctcgtgct ttgaccggcc gttccccggg aagggggcgg gcgggacggg
    17881 aactgcgcgg ccgcgaccac cgtcggtggc gccgcggcgg cggatcgata ggcaagacgg
    17941 gggcggaagc cgccccccag gaggcaggcg catgaagaaa gccatagcca tcgtcgcggc
    18001 cctcttgacc gcggcatcgg ccttcggcca ggggaccatc aagatcggcg gcatctggcc
    18061 gctctccgac atcaccggga agcagggcag catggccgcc cagctcgccg tcgccgagat
    18121 caacgccgcc ggcggcgtcc tcggcaggca gctcgagctc accgtcgtgg acgacgagct
    18181 caaggccgac aaggcggccg cggccgtcga gaagctcgtc accgtggaca acgtcgaggt
    18241 cctagtgggc ggcatgggct ccggagccga gctcggcaag atcccggcct tcaagaagta
    18301 cggcaaggtc gtcatgtcca ccggcgcggc cagctacacg gtggagaacg ccctgggcgc
    18361 cgacgccaac tggtacttcc acctccatcc ctgggactac aaccagggcg cctcctacgc
    18421 cgagggctgg gacgcgatcc aggccgcgta caagaacgtc aagatcaaga agatcttcct
    18481 cgcctacgag gagggcgcct tcggcaaggc ctcctgggac gcgaccaaga agctcttcgg
    18541 cgagagcggc cgctacgtca tcgacggggc gcccttcaag agcgcggccc tgggcggcgg
    18601 cgactacgcg gcggtcctgg aggcggccaa ggacttcaag cccgacctct acctctgggc
    18661 cggctacgac gccgacgccc tccccatgct cgagcaggcc aaggcgatga agttcagccc
    18721 cggtatctac ctcggggcgc cccccggatg gcccatcggc ttcgggacct ccaagctct
//
LOCUS       DAOOSJ010000016        18114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2120_length_18114_cov_6.727006, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000016 DAOOSJ010000000
VERSION     DAOOSJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_18114_cov_6.727006"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLG14_01795"
     CDS             <1..906
                     /locus_tag="PLG14_01795"
                     /inference="COORDINATES: protein motif:HMM:NF019877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HOX47890.1"
                     /translation="PDCRGYEIEEGEWKLKGYEGPVLACDKCGSEMQLKSGRFGKYFG
                     CTNPDCKNTRKLLRNGQAAPPKSDPVPLPELPCEKVDDYYLLRDGAAGLFLAASKFPK
                     NRETRAPLVRELVPHRAEIAEKYRFLLDAPAEDPEGRPAVIRYSRKTAEHYVQTEIEG
                     KPSGWKAFFKDGAWVAEAAGAAGHDAAARRGAKAPGGGGKPAAKAAAKKPAAKKAAAP
                     APAKKVAAPKAKAATTAKKTAAEKPAAAKPAAKKAEASKAKAGSAAKSSAAKSSAAKP
                     AAKKTAAKPAATKAKPAAKKSAKKA"
     gene            complement(996..1439)
                     /locus_tag="PLG14_01800"
     CDS             complement(996..1439)
                     /locus_tag="PLG14_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47891.1"
                     /translation="MRDVAPDHWQYRGDEWTEEAFAAWRAENDGGTSLWRLAWDGDRV
                     AGTVLSGLDAEGNARRGRRRGLTEHVFVRKEWRGKGVATALLADALKALEREGMEEAF
                     LGVDEENPSGAMRFYESLGYRAVRKDTWHRKWLVEPEGAREAWLY"
     gene            complement(1446..2009)
                     /locus_tag="PLG14_01805"
     CDS             complement(1446..2009)
                     /locus_tag="PLG14_01805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX47892.1"
                     /translation="MAVGGIEFRGYRGEADLPGIVEAVNRSLESDGVDDRLGAEDALE
                     GFGKPGPRRDPLKDVFLALAEGRVVGVSRTGWYEELEGKRMYCVTGYLEPGLRGKGVF
                     RALLRLAEGRARELAAGHPAGARKILQAWSAGPEADWPALLASEGYREVRHFWDMLRP
                     IDGTVPEGRLPPGLESRPARPEDLRAV"
     gene            complement(2099..2848)
                     /locus_tag="PLG14_01810"
     CDS             complement(2099..2848)
                     /locus_tag="PLG14_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX47893.1"
                     /translation="MKVITLDEGFLVYQFDPPEGELEGLNFLVLVEDGKALFLDTGYR
                     ENMEEALADLGARGAVPVSAIVSHYHPDHAGGLALLGVPEVWAGEGFRKTAEPWLRPG
                     ETIAVEPTRIVSAPTELRFGKRRLELFPLPGHSDDSLAVVVDGSWIYAADAILLTNDG
                     RPVLPSVHSRPVSLHLQAVDWLAARTGLVFVPGHGAVMGEKALRERDLANRRRYLSAI
                     AGAKGRVSLEAATAGCEPPFLGEAWHEENYL"
     gene            3061..3981
                     /locus_tag="PLG14_01815"
     CDS             3061..3981
                     /locus_tag="PLG14_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOX47894.1"
                     /translation="MRYLLIANPASGAGGRPDRAERVVEWFRQRGDEVVARRTSGPGD
                     ATRLARGAEGFDAVIAAGGDGTVNEVLNGLAGGDLPLGILPWGTGNVFAKEMGFPRGL
                     RRCCRVIRRGLSARLDLGRCGERRFLMMAGAGIDAYALGRMRGKNWKRLFGVAGYAVG
                     ALLAFARYRDAEIEVRFPDGARLKGSYVLVSNTRLYGGVFAFAPTAVPTDGLLDVFVF
                     KGRSRWGLLGFLVRRILLPGRLFRGGALVKTASVELRSAAAVPLQVDGDPFAPLPRSL
                     SVEPAALSVLLPKRTLKRLGARARSSLRER"
     gene            complement(4029..4748)
                     /locus_tag="PLG14_01820"
     CDS             complement(4029..4748)
                     /locus_tag="PLG14_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47895.1"
                     /translation="MKKIALSIALLALAGLAFAQAPAAPTLSIDYQVNAVAADDANNY
                     LTFKGPAASANKDQFDAVSGASKLESTFVLNYYRNDIFGKKILPGGLRGLFLYPIAGD
                     KTRLEDALNVTKLADGSIRVRYVHRGTANEIVTDKAGKLVFPGATVQARKIGYIVGEG
                     PQVISNEFSADGTAAKVDWAKVWDKNIAGGKAIGATTNKTGDITADVANSELVGYEGA
                     LQFAFDGKILKIAGDLKILKK"
     assembly_gap    4779..4878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5016..5549
                     /locus_tag="PLG14_01825"
     CDS             5016..5549
                     /locus_tag="PLG14_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOX47896.1"
                     /translation="MKAESVSRIDGFLASSTLELFASHGVAVHEVDFRHQGIEEPFAS
                     TIGFTAERILGVLVLTVSRGLAEASLPESLRVPGVADEIVADWAGELSNQFLGRLKNR
                     FYAAGVEIALSTPTVFAGKELRHFAHPSPLYRSLYFEGRGSLLVEFQADYGEDFELGE
                     EVEGRDEGPPEGEALFF"
     gene            5644..6201
                     /locus_tag="PLG14_01830"
     CDS             5644..6201
                     /locus_tag="PLG14_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOX47897.1"
                     /translation="MTKLHPRLGGRRATKAVLVPILALLAFLLAAACAAPPPAPAAAP
                     AAPEPQLREPLFRVSSIRILRDRLVTTELLLGFEVENPNDFPLALESCSWSLEAEGKP
                     WVKGSLGGGAEEPLVAPAGATIVRELEVELNFADSDRRLFDLIARLGSVRYRLRGEAL
                     LSAGGGASRTLAARFDVEGSCAVER"
     gene            6308..8047
                     /locus_tag="PLG14_01835"
     CDS             6308..8047
                     /locus_tag="PLG14_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX47898.1"
                     /translation="MTDGLTFFLISLPEEIAVIFLTLSIAGIKAESRLRRFLLTGSLL
                     AVAFFLAFKYLLPFGLALEAAVQVAVLAAVLRFAWKIPLSFALIDSGITFTFILAIQA
                     IFVAMTPLALAQAFASPFRESLTRYLLAWLEISILLGFSLAIRRLSAGSGSPRDFSTV
                     LRMRLGGWKAAAFSGQVIFLVALLGVNLNTIMDLRLRPEGDPASAGFLVILTSLTLLF
                     GVASLVVYVIVIKEISARQESQRVLEDLVAKLPGIVYRRRGPPSMATEYAHFGSLDLA
                     ERLPPAKVGVKGVSWRDFVLPDDRPAFESALSLSAEGSGEYSLEYRLDVSGEPPFWVW
                     DRGTTVRAADGGEVIMEGYASDISARKIAEEGLAAANREKECLVRELFYRTGNSMQVI
                     SSMMRLRAAASGEERIESMFADISLRVDALSIAQDALRASRDLSTLDLGFYIADLVDS
                     LVFRTAWLAGVVRPVLDLESATISIDDAGSLGLVLTELLLNAARYAAAGREGCGVTIK
                     SRLESSGWIRIEFEDDGPGLPEGFDLGRLRSLGLPLAASLVRDQLKGEFELSKGPGLR
                     CLVRFPAVCERRV"
     gene            complement(8088..>8347)
                     /locus_tag="PLG14_01840"
     CDS             complement(8088..>8347)
                     /locus_tag="PLG14_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47899.1"
                     /translation="APDPAGSAGQSLLGLKVFAFAEAGWACGKASSTISLEPYVRSVL
                     ATGPEILRGAPAGARQGTEWGSAFSISLGLRLAWTRMRLPG"
     assembly_gap    8348..8447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8448..8656)
                     /locus_tag="PLG14_01845"
     CDS             complement(<8448..8656)
                     /locus_tag="PLG14_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47900.1"
                     /translation="MRPAPVRAVAALSAALLAGCSGMVVMDPAAWTDAIPASLAALPT
                     KPDGVYRGSYTIALPAGAFAAFRHV"
     gene            complement(8653..9936)
                     /locus_tag="PLG14_01850"
     CDS             complement(8653..9936)
                     /locus_tag="PLG14_01850"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX47901.1"
                     /translation="MKRASRMAIAVTAFASTIFALAGCQSAPRAGSMVGGWVAPGFEG
                     VREEFERNFAERGESGAACAVYWKGEKVVDLWGGWRDAKTKDPWKEDTMVLVFSVTKG
                     MAAMTFAHARSRGLLDYDERVAAYWPEFAQAGKESITVRQLLSHKAGLVLFDEALPAD
                     RLMDRDFVSPFLARQKPYWVPGEQHGYHSATIGIYMGELLRRVDPAGRSLGRYFAEEI
                     AAPLGLEFYIGLPAEIPDSRVATLIMASPAGGLINLGKLPKGMRAKMLDFDSYFMRSF
                     GIPKGYDPATRAGWSIELASGNGIGTARSMAKAYAEFATGGKTLGIGPETLALLEAPP
                     AAPPAGPLDEVLGFDSWYSLGYCRPGPSLSFGSSPKAYGTPGAGGNFAFADPDARLGF
                     AYQMTKMGYHASDDPREKALRDAVYAAIRAQEDRK"
     gene            complement(9923..10156)
                     /locus_tag="PLG14_01855"
     CDS             complement(9923..10156)
                     /locus_tag="PLG14_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47902.1"
                     /translation="MDRESYEIRVRGSFEPGWADWFGDRELREEEGDTLLLLRDVDNA
                     AFYGAMGILGALDRRVVSVRVCDPCRRRNHEES"
     gene            complement(10232..>12799)
                     /locus_tag="PLG14_01860"
     CDS             complement(10232..>12799)
                     /locus_tag="PLG14_01860"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX47903.1"
                     /translation="PAGFGKSSLAMQYLEALAAAPSDAAEAAQAKPQPRAAWVSLERE
                     DDDPVRFWGAAAAAIELAAADPAEISPFAPLLSAARAPQPPSARELAAALAEAVDSLD
                     SFLLLVLDDFQLISSKEILDSFARFVEHSPERLRILLLSRRDPPLPLARLRAGGFVAE
                     LRADDLRFGPDEAASLLHGAAGGRLSPEQASEVADKTEGWGAGLRLAAISLGRKPDVE
                     AFIRGFSGSDRLILEYLTEEVLGLQSEETRSFLLRSAVLDRFCAGLCDAAIGPAGGSA
                     AMIGRIEAAGLFLVPLDDEGRWHRYHHLFAETLRARLRALAPGVEAEVLAKAATWHES
                     SGNPETALELQLRAGNKAEAARLLEGIDFLRRGELATLDRHLEAVGEAEIRKRARLDD
                     LAAWVRFFQGRIPEAESWMAKTAGDADQFGGKEGAYLRGSHAALRAFILLLRGRNQEA
                     AAEAKAAEADLAPGDYYPLGVAFFALGAALRAEGRFKAARAEFERFRALGEAYDSPWS
                     VAVARYELGTTAALEGRLGEAERLFEEALAEAAKGKAPFGSVTKLRAAYADLLYERGE
                     AGRAASQAAEALAATQPGVNPNTGLEVYAIQIRLLLSRRELEGAGRFFERAAELEGRG
                     GILPRLEASIRELRTRAALLSGSAEASPWPIPERWLGDAAAMGTARSCEIRRLLARGN
                     PAEALAGAQSLADEAAAGGWGRLFVTAKAQEALALAGAGREEEALAALGQSLEAGHSE
                     GFFRSYLDLDGDARRLLSAFVRRREGAGPAAGLAARLLAAKESGGAPKPALASVLSAR
                     ELEVVGLLAEGLSNRAIAERLFVSESTVKTHLYHIAAKLEAPNRVAILARARELGLE"
     assembly_gap    12800..12899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13207..14403)
                     /locus_tag="PLG14_01865"
     CDS             complement(13207..14403)
                     /locus_tag="PLG14_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47904.1"
                     /translation="MRIQPTVAGKEARSIMGDAPRLALRLMPAFFAALALTVAFPGAF
                     AAWGKLRGIAFAAGIELLGLGLLFQASAAFELVRAWRAGRLATSGAYSLSRNPSFSWW
                     IFSVIPSFSLLLDSWLFLPVALLFRIVAGKAAEAEERELHALFGQDYADYRRRTRAFL
                     PLPRLLPLGLRRSLRATLGLAVLGCLALGIYLAVAGPAMRGFGASSSEEAARMPGDEY
                     VGLDAAGYTQGIDIDAPPDQVWKWLVQIGYKRAGWYNIDLVNRLADPAYFHDARASAL
                     RIIPEFQSAREGDKIHLAPALGMTIAMAEVPRFLVLVAEPDSLGGNNVAWTFAVEPLP
                     KGRSRLLARFRIQMGEDFASRAAAWFMNDLAGATVQQPAMLWGLKMRAERRLPYVTPQ
                     GHRTGE"
     gene            complement(14930..>18114)
                     /gene="nifJ"
                     /locus_tag="PLG14_01870"
     CDS             complement(14930..>18114)
                     /gene="nifJ"
                     /locus_tag="PLG14_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOX47905.1"
                     /translation="DHQDVMAARQTGWAMLGANNVQEVMDMAIVAHAATLEARVPFLH
                     FFDGFRTSHEVQKVEEMSYDVMRQMVSDELVRAHRARGLNPETPSIRGTSQNPDVYFT
                     AREAANKLYAAVPAIVEKQMEKLGKLVGRNYKLFEYVGAPDAERVVIVMGSGADTVEE
                     VVNHLVKKGEKVGAVKVRLFRPFSVEHFMKALPATVKSIAVLDRTKEPGSIGEPMYED
                     VRTAIGEAQGSGKAQFKGWPTVIGGRYGLGSAEFYPCHAKAVFDELAKPAPKNHFTVG
                     INDDVTGTSLAYDEGFQLEGDGVKECLFYGLGSDGTVGANKNSIKIIGDETDNSAQGY
                     FVYDSKKAGTYTISHLRFGPKQIKKSYLIGKADFIACHKFSFLEKLDILANAKDGGVF
                     LLNSPFSADKVWDEIPVEVQKRIIEKKLKFYVIDGMEIAEKAGMGGRVNTVMQTAFFK
                     ISGVLPEAEAVKLVKKYAEKTYARKGADVVAKNIAAIDLALGATHEVKYPAQATSKHS
                     MKLPVPATAPKFVREVIGEMIAARGEKLPVSKLPMDGTFPTGTTKYEKRNIAEKIPVW
                     NSEICIQCGNCTMVCPHACIRLKAYEPKHAEGAPKTFKSVDAKGKELAGLKATLQVAP
                     EDCTGCGACVNICPVADKAAAGRKAINLEPQLPLREAEAANWEHFLKIPNTDAKLLNL
                     ATPKGIAMKQPLFEFSGACAGCGETPYIKLMTQLFGDRAVIANATGCSSIYGGNLPNT
                     PYAFREDGRGPAWSNSLFEDAAEFGLGMRLSADKLAEYARELLSANKGKGVPADLADK
                     LLSNKLASDEDIAQQRAWIAELKAACGKSAEGWAKELANVSDNLIRKSVWIFGGDGWA
                     YDIGFGGLDHVIASGRNVNLLVMDTEVYSNTGGQASKATPFGAVAKFAAAGKDIGKKD
                     LGMIAMSYGYVYVASIAMGANMAQAIKAFREAESYDGPSIIIAYSHCAMHGIDMMKGQ
                     NQQKLSAEAGIWPLYRYDPRLKAEGKNPFQLDAKEPDTSKIEQFMYSEVRFKTLRDAD
                     QKRAAELLAAEKAFVERRWKEYKYLSERPF"
BASE COUNT         2517 a         6061 c         6390 g         2846 t
ORIGIN      
        1 cccgactgcc gcggctacga gatcgaggag ggcgagtgga agctcaaggg ctacgagggc
       61 cccgtgctcg cctgcgacaa gtgcggcagc gagatgcagc tcaagtcggg ccgcttcggc
      121 aagtacttcg gctgcaccaa ccccgactgc aagaacacgc gcaagctcct caggaacggc
      181 caggccgcgc cgcccaagag cgatcccgtg cccctgcccg agctcccctg cgagaaggtg
      241 gacgactact acctcctgcg cgacggcgcg gccggcctct tcctcgcggc cagcaagttc
      301 ccgaagaacc gcgagacccg cgcccccctg gtgcgtgagc tcgtccccca ccgcgcggag
      361 atcgcggaga agtaccgctt cctcctggac gcccccgcgg aggaccccga gggccggccc
      421 gcggtgatcc gctactcgcg gaagacggcc gagcactacg tgcagacgga gatcgaggga
      481 aagccttcgg gctggaaggc cttcttcaag gacggcgcct gggtcgcgga ggccgctggc
      541 gccgccggcc acgacgcggc cgcgcggcgc ggggctaagg cccctggcgg cggcggcaag
      601 ccggcggcca aagccgccgc gaagaagcct gccgcgaaga aggccgcggc cccggccccg
      661 gctaagaaag tcgcagctcc caaagccaag gccgcgacga cggcgaagaa aaccgccgcc
      721 gagaagcctg ccgccgcaaa acccgccgcg aagaaggccg aggcgtccaa ggccaaggcc
      781 ggctctgccg ccaagtcgag cgccgccaag tcgagcgccg cgaagcccgc cgcgaaaaaa
      841 accgcggcca agccggcggc gacgaaggcg aagcccgccg ccaagaaatc ggccaagaag
      901 gcctagccgg gcgtccccgg ccgggccgcc ggcttccgcc tgaccggcgc gaagcgggcg
      961 gctcgagccg gcgcggcctt cggcgcagcc gacgctcagt agagccaggc ctcccgcgcg
     1021 ccttcgggct cgacgagcca cttccggtgc caggtgtcct tcctcacggc cctgtagcct
     1081 aggctttcgt agaatcgcat ggcgccgctc gggttctcct cgtccacgcc gagaaacgcc
     1141 tcttccatcc cctcccgctc cagggccttg agggcgtcgg cgaggagggc cgtcgccacg
     1201 cccttgcccc gccactcctt gcgcacgaag acgtgctcgg tcaggccccg cctgcggccg
     1261 cgccgcgcgt tgccctcggc gtccaggccc gagaggacgg tgcccgccac gcggtccccg
     1321 tcccaggcga gccgccagag ggaggtcccg ccatcgttct ccgcgcgcca ggcggcgaag
     1381 gcctcctcgg tccactcgtc cccgcggtac tgccagtggt cgggggcgac gtcgcgcaac
     1441 gtttcctaca ccgccctgag gtcctccggc cgcgcgggcc tggactcgag gcccggcgga
     1501 agccttccct cggggacggt cccgtcgatc gggcgcagca tgtcccagaa gtgcctgacc
     1561 tcgcggtagc cctcgctcgc gaggagggcg ggccagtcgg cctcggggcc ggcgctccag
     1621 gcctggagga tcttcctcgc gccggcgggg tggccagccg ccagctcccg cgcgcgtccc
     1681 tcggccaggc ggaggagggc gcggaagacg ccctttcccc ggagcccggg ctcgaggtag
     1741 cccgtcacgc agtacatcct cttgccctcg agctcctcgt accagcccgt cctggagacg
     1801 ccgacgactc ttccttccgc gagggcgagg aagacgtcct tcaaggggtc gcgccgtggc
     1861 ccgggcttcc cgaatccttc gagcgcgtcc tccgccccga gcctgtcgtc gacgccgtcg
     1921 gactcgaggc tcctgttgac cgcctccacg atgccaggaa gatcggcctc cccgcggtat
     1981 ccgcggaact cgatcccccc gacggccaag ccctcaggct cgaggaccgg cgttctcgat
     2041 gcccgggcct gggccgcgcc ggggtcgcgg cccggggcat cggcacgccc gtccgcgctc
     2101 atagatagtt ctcttcgtgc caggcctcgc ccaggaaggg cggctcgcag ccggccgtcg
     2161 ccgcctcgag gctgacgcgg cccttcgcgc cggctatcgc cgaaaggtag cggcgccggt
     2221 tcgcgaggtc ccgctcccgg agggccttct cgcccatcac cgcgccgtgg ccgggcacga
     2281 agacgaggcc ggtcctcgcg gcgagccagt cgaccgcctg gaggtggagg gagacgggcc
     2341 tcgagtggac ggagggcagg acgggccggc cgtcgttcgt gagaaggatg gcgtccgcgg
     2401 cgtagatcca ggagccgtcc acgacgacgg cgagggagtc gtcgctgtgg ccggggaggg
     2461 ggaagagctc gagccgccgt ttcccgaagc ggagctccgt cggggccgag acgatccgcg
     2521 tgggctcgac ggctatggtt tcccctggcc ggagccaggg ctccgccgtc ttcctgaagc
     2581 cctcccccgc ccagacctcg gggacgccca ggagggcgag ccctcccgca tggtcgggat
     2641 ggtagtgcga gacgatggcc gaaaccggga ccgcgccccg cgccccgagg tccgcgaggg
     2701 cctcttccat gttctcgcgg tagcccgtgt cgaggaagag ggccttgccg tcctcgacga
     2761 ggaccaggaa gttgaggccc tcgagctcgc cctcgggcgg atcgaactgg tacacgagga
     2821 agccttcgtc gagcgttatg accttcatcg cctctccttg gggcagggtg tcgaggaagg
     2881 ctccggcccc ggctgatccg gtccggcgcc tggtccaggc ggctcaagta tagcctcccc
     2941 gcgccgccgg tcgagccccc gcctgggcgc ttgggccgcg cggccgaccg cgcctggcgc
     3001 gcggggcaag gcgcggacgc tctccttgcg gccccccgcg gcctcgcgta tgcttctccc
     3061 atgcgctatc tcctcatcgc caatcccgcc tcgggcgccg ggggccgtcc ggaccgggcg
     3121 gagcgcgtgg tcgaatggtt ccgccagcgt ggcgacgagg tcgtggcgag gcggacgagc
     3181 gggcccggcg acgcgacccg cctcgcgcgc ggggcggagg gcttcgacgc cgtgatagcg
     3241 gcggggggcg acgggacggt caacgaagtc ctgaacggcc tcgcgggcgg ggatctgcct
     3301 ctgggcatcc tgccctgggg gacggggaac gtcttcgcga aggagatggg cttcccgagg
     3361 ggcctcaggc gctgctgccg cgtcatccgc cgcggccttt cggcccggct cgacctgggc
     3421 cgctgcgggg agcgccgctt cctcatgatg gccggcgccg gcatcgacgc ctacgccctg
     3481 gggcggatgc gcggcaagaa ctggaagcgg ctcttcggcg tcgcgggcta cgcggtcggg
     3541 gccctcctcg ccttcgcccg ctaccgcgac gcggagatcg aggtccgctt tcccgacggc
     3601 gcgcgcctca agggcagcta cgtcctcgtc tccaacacgc ggctctacgg cggcgtcttc
     3661 gccttcgcgc cgaccgccgt gccgaccgac ggcctcctcg acgtcttcgt cttcaagggg
     3721 aggagccgct ggggcctcct gggcttcctg gtccgccgga tactccttcc cggacggctc
     3781 ttccgcggcg gcgccctcgt gaagaccgcc tccgtggagc tgcgctcggc cgcggcagta
     3841 cccctccagg tcgacggcga ccccttcgcc cccttgccgc gctctctctc cgtcgagcct
     3901 gccgcgctct ccgtcctctt gcccaagcgg accctcaagc ggctcggggc ccgcgcgcgc
     3961 tcctccctcc gcgagaggta aaaaaacgcc ccgggcgggg gcccggggcg cggtctccaa
     4021 gggagtgctt acttcttgag gatcttgagg tcgccggcga tcttgaggat cttgccgtcg
     4081 aaggcgaact ggagggcgcc ctcgtagccg acgagctcgg aattcgccac gtcggcggtg
     4141 atgtcgccgg tcttgttggt ggtggcgccg atcgccttgc cgcccgcgat gttcttgtcc
     4201 cagaccttgg cccagtcgac cttggccgcg gtgccgtcgg cggagaactc gttggagatg
     4261 acctgggggc cctcgccgac gatgtagccg atcttgcggg cctggacggt cgcgccgggg
     4321 aagaccagct tgccggcctt gtcggtgacg atctcgttgg ccgtgccgcg gtggacgtag
     4381 cggacgcgga tggagccgtc ggcgagcttc gtgacgttca gggcgtcctc gaggcgggtc
     4441 ttgtcgccgg cgatggggta gaggaagagg ccgcggagcc cgccggggag gatcttcttg
     4501 ccgaagatgt cgttgcggta gtagttgagg acgaaggtgg actcgagctt ggaggcgccg
     4561 gagacggcgt cgaactggtc cttgttcgcg gaggcggcgg gacccttgaa ggtgaggtag
     4621 ttgttggcgt cgtcggcggc gacggcgttg acctggtagt cgatggagag ggtgggagcc
     4681 gcgggggcct gcgcgaaggc gaggcccgcg agggcgagaa gggcgatgct gagggcgatc
     4741 ttcttcattg cttcctcctg gaaggtatgg tgatgcggnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnngc cgcttggtcc ggcgccatgg ccgcgaagcc gcggcggcgc
     4921 tgtggcggcg cggcgcagcc gtggtgctgc ggccgctgtg ttgtgtcggc ggcgctgcga
     4981 agccgcggcc gagccgcgaa gaggaggtcc atccgatgaa ggccgagtcc gtttcccgaa
     5041 tcgacggctt cctcgcgtcc tcgaccctcg agctcttcgc ttcgcacggc gtcgccgtcc
     5101 atgaggtgga tttccgccac cagggcatcg aggagccctt cgcctcgacg atcggcttca
     5161 ccgcggagcg catcctgggc gtcctcgtcc tcacggtgag ccgcggcctc gcggaggcga
     5221 gcctgcccga gagcctccgc gtgcccggcg tcgccgacga gatcgtcgcg gactgggcgg
     5281 gcgagctctc caaccagttc ctgggcaggc tcaagaaccg cttctacgcc gcgggcgtgg
     5341 agatagccct ctcgacgccg accgtcttcg cgggcaagga gctcaggcac ttcgcccacc
     5401 cttcgccgct gtaccggagc ctctacttcg aagggcgggg ctcccttctc gtggagttcc
     5461 aggccgacta cggcgaggac ttcgagctcg gcgaggaggt cgagggacgg gatgaggggc
     5521 cgcccgaggg ggaggcgctg ttcttctgag cgccgcccgt cgagcgccac ccttcgcctg
     5581 tcgcctatcg cccgcggccc ttcgggatgg gccggcgagc cctcgatgcg ctatgctctt
     5641 tgcatgacca agctccaccc gcgcctcggc ggccgccgcg ccaccaaggc cgtcctcgtc
     5701 ccaatcctgg cccttctcgc cttcctcctg gccgccgcct gcgccgcccc tcctcccgcg
     5761 cccgccgcgg ctcccgctgc gcccgagcct cagctccgcg agccgctctt ccgcgtgagc
     5821 tccatccgga tcctcaggga caggctcgtg acgacggagc tcctcctcgg cttcgaggtg
     5881 gagaacccca acgatttccc gctcgccctg gaatcctgct cctggagcct cgaggccgag
     5941 ggaaagccct gggtgaaggg cagtctgggc ggcggcgcgg aagagcccct cgtcgcccca
     6001 gccggcgcga ccatcgtgcg ggagctcgag gtggagctca atttcgccga ctcggaccgg
     6061 aggctcttcg acctcatcgc ccgcctgggc tcggtccgct accgcctccg cggcgaagcc
     6121 ctgctctccg ccggcggcgg cgcgtccagg actctcgcgg cgcgcttcga cgtggaggga
     6181 agctgcgcgg tggagcgctg agggagacga gtgtcgcggg cagcgccgat tggcgctaga
     6241 ctggaacaag tcctacgggg aatcgggcga gccgctgttt cgacgagcgg ccaagtgagg
     6301 cggcaggatg acggatggtc tcacgttctt cctcatctcc ctccccgagg agatcgcggt
     6361 catcttcctc accctctcga tcgcgggcat caaggccgag tctcgcctcc ggcgcttcct
     6421 cctgacgggc tccctgctcg cggtcgcctt tttcctcgcc ttcaagtacc tccttccttt
     6481 cgggcttgcg ctcgaagcgg ccgtccaggt ggcggtcctc gcggccgtgc tccgcttcgc
     6541 atggaagatc cctctctcct tcgccctcat cgactcaggc atcaccttca ctttcatcct
     6601 cgccatccag gccatcttcg tcgccatgac ccccctggcc ctggcccagg ccttcgcctc
     6661 gcctttccgc gagtcgctga cgcgctacct cctggcttgg ctggagatat ccatcctcct
     6721 cggcttcagc ctggctatcc gccgcctttc ggcagggtcc ggctccccgc gggacttcag
     6781 caccgtcttg cgcatgcggc tcggcggatg gaaggccgcg gccttctcgg gccaggtcat
     6841 cttcctggtc gccctccttg gggtgaacct caataccatc atggacctcc gcttgcgccc
     6901 ggagggcgac cccgcctccg cgggcttcct cgtgatcctc acgagcctca ccctcctctt
     6961 cggcgtggcg agcctcgtcg tctacgtgat cgtcatcaag gagatctccg cgcggcagga
     7021 gtcccagcgc gtccttgaag accttgtcgc gaagctgccc ggcatcgtct atcgccggcg
     7081 aggccctccc tcgatggcga cggaatacgc ccatttcggc tcccttgacc tcgccgagcg
     7141 gcttccgccc gccaaggtcg gcgtcaaggg cgtctcctgg cgcgacttcg tcctgccgga
     7201 cgaccgcccc gccttcgaga gcgccttgag cctttccgca gagggttcag gggagtattc
     7261 cctggagtac cggctcgacg tctccgggga gccgcccttc tgggtctggg accggggaac
     7321 gacggtccgc gcggccgacg gcggcgaggt catcatggag ggctacgcct ccgacatcag
     7381 cgccaggaag atcgccgagg aggggctcgc cgccgcgaac agggagaagg aatgcctcgt
     7441 gcgcgagctc ttctaccgga ccggcaactc gatgcaggtg atctcctcca tgatgcgcct
     7501 tcgcgccgcg gcctcgggcg aggagaggat cgagtccatg ttcgccgaca tctcgctccg
     7561 cgtggacgcc ctctcgatcg cccaggacgc cttgcgcgcc tcccgggacc tctcgaccct
     7621 cgacctgggc ttctacatcg cggacttggt ggacagtctc gtcttccgca ccgcctggct
     7681 cgccggcgtg gtgcgccccg tcctcgacct tgagagcgcg accatctcca tcgacgacgc
     7741 ggggtcgctg ggcctcgtcc tgaccgagct cctcctcaac gcggcccgct acgccgcggc
     7801 gggcagggag ggctgcggcg tcacgatcaa gtcgagactg gaaagtagcg gctggatccg
     7861 catcgagttc gaggacgacg gtcccggcct tcccgaaggc ttcgacctcg gtcggctgcg
     7921 cagcctcggc ctgccccttg ccgcgagtct cgtgcgggac cagctcaagg gggaattcga
     7981 gctgtcgaag ggtcctggtc tccgctgcct ggtccgcttc cccgcggtct gcgagcgccg
     8041 ggtctgaagg ccgggatcaa gacccgggtc tgtcaggcgg agcggagtca tccgggaagg
     8101 cgcatccgcg tccaggccag gcggaggccc agcgagattg aaaaggcgct tccccattcc
     8161 gttccttgcc gcgcgccggc gggcgcgccg cgcaggatct ccgggccggt cgcgaggacg
     8221 ctcctgacat agggttcgag actgatcgtc gaggaagcct tgccgcaggc ccaccccgcc
     8281 tcggcgaagg cgaagacctt gaggccgagg aggctctggc ccgccgagcc ggccgggtcc
     8341 ggcgcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg acatggcgga
     8461 aggcggcgaa ggcgccagcc gggagggcga tcgtgtagga gccccggtag accccgtcgg
     8521 gcttggtggg aagcgccgca agggaggccg ggatcgcgtc ggtccaggcc gctgggtcca
     8581 tgacgaccat gcccgagcag ccggcgagaa gcgccgcgga gagcgccgcg acggcgcgga
     8641 ccggcgcggg cctcatttgc ggtcctcctg ggcgcggatg gccgcgtaga cggcgtctcg
     8701 aagggccttc tcccgcggat cgtcgctcgc gtggtagccc atcttcgtca tctggtaagc
     8761 gaagccgagc ctggcgtcgg ggtcggcgaa ggcgaagttg ccgcccgcgc ccggcgtccc
     8821 gtaggccttc gggctcgagc cgaaggagag cgaggggccg ggccggcagt agccgaggga
     8881 gtaccaggag tcgaagccga ggacctcgtc gagcggaccc gcgggaggag ccgcgggcgg
     8941 cgcctcgagg agggcgaggg tctccggccc gatcccgagc gtcttgccgc ccgtcgcgaa
     9001 ttctgcatag gccttcgcca tcgagcgcgc ggtgcctatg ccgttgcccg aggcgagctc
     9061 gatgctccag ccggcgcgcg tggcgggatc atagcccttg gggatgccga aggacctcat
     9121 gaagtaggaa tcgaagtcga gcatcttggc ccgcatgccc ttgggcagct tcccgaggtt
     9181 gatgaggcct cccgcggggc tggccatgat gagcgtcgcg acgcgggagt cgggaatctc
     9241 cgcgggtagg ccgatgtaga actcgaggcc gagcggagcg gctatctcct cggcgaaata
     9301 ccgcccgagg ctgcggcccg cgggatcgac tctgcgcagg agttcgccca tgtagatgcc
     9361 gatcgtcgcg gagtggtagc cgtgctgctc gcccggaacc caatacggtt tctgcctcgc
     9421 gaggaatggc gagacgaagt cccggtccat gagcctgtcg gccggcaggg cctcgtcgaa
     9481 gaggacgagg ccggccttgt gcgagaggag ctggcggacc gtgatggatt ccttgcccgc
     9541 ctgggcgaac tccggccagt aggcggcgac tctctcgtcg taatcgagga ggccgcgcga
     9601 gcgggcgtgg gcgaaggtca tcgcggccat gcccttggtg acggagaaga cgaggaccat
     9661 cgtgtcttct ttccacggat ccttggtctt cgcgtcgcgc cagccgcccc agaggtcgac
     9721 caccttttcg cccttccaat agacggcgca ggcggctccc gattcgccgc gttcggcgaa
     9781 gttgcgctcg aattcctccc gcacgccctc gaatcccggg gcgacccagc ctccgaccat
     9841 gctcccggcc cggggcgcgc tctggcagcc ggcgagggcg aaaatcgtcg acgcgaacgc
     9901 ggtcacggcg atggccatcc gactagctct cttcatgatt cctccgtcgg cagggatcgc
     9961 agacgcggac ggagaccacc cgccgatcaa gggcgccaag gatgcccatg gcgccgtaga
    10021 aggcggcgtt gtcgacgtcg cggaggagga ggagggtgtc cccttcctcc tcgcggagct
    10081 cgcggtcgcc gaaccagtcg gcccagccgg gctcgaagga gcctcgcacg cgaatctcgt
    10141 agctttccct gtccatggcc cgagcatggc ggtagcccgc gggccgtgga atcggctgaa
    10201 agtatgattc ggaggatcgg aggagggtga cttattccag tccgagttcg cgggcgcgcg
    10261 ccaggatggc cacgcggttc ggggcctcga gcttggccgc gatgtggtag aggtgggtct
    10321 tcacggtgga ctcggagacg aaaagccgct cggcgatggc gcggttggag agtccttcgg
    10381 cgaggaggcc cacgacctcg agctcgcggg cgctgaggac gctggcgagg gctggcttgg
    10441 gcgcgccgcc gctctccttg gccgccaaga gccttgccgc gaggccggcg gccggcccgg
    10501 ctccttcgcg gcggcggacg aaggcggaga ggaggcgccg cgcgtcaccg tcaagatcaa
    10561 ggtagctgcg aaaaaagcct tcggaatggc cggcttcgag gctttggccc agggcggcga
    10621 gcgcctcttc ctcgcgcccc gctccggcta gggccagcgc ctcctgggcc ttggcggtca
    10681 cgaagagcct gccccagccg cccgccgcgg cttcgtccgc gaggctctgc gcgcccgcta
    10741 aggcttcggc cgggtttcct cgcgcgagga gcctgcggat ctcgcagctt ctggccgtgc
    10801 ccatcgcggc ggcgtccccc agccaacgct cagggatcgg ccagggcgac gcttccgcgg
    10861 atcctgagag cagggcggcg cgggtccgca gttcccggat cgacgcctcg aggcgcggca
    10921 ggattccgcc gcggccttcg agttcggccg cccgctcaaa gaaccggcca gccccttcga
    10981 gttcgcgcct cgaaaggagc agacgtattt ggatggcgta gacctcaagc cccgtgttcg
    11041 ggttgacccc gggttgcgtg gcggccaggg cctccgccgc ctggctcgcg gcgcgcccgg
    11101 cctctccgcg ctcgtagagg aggtccgcgt aggccgccct gagcttcgtc acggagccga
    11161 agggagcctt gcccttcgcg gcctcggcca gcgcctcctc gaagaggcgc tccgcctcgc
    11221 cgagcctgcc ctctagggcg gcggtcgtgc cgagctcgta gcgagcgacg gcgaccgacc
    11281 agggactgtc ataggcctcg cccagggccc ggaagcgctc gaactcggcg cgcgcggcct
    11341 tgaaacggcc ctccgccctg agcgcggcgc cgagcgcgaa gaacgcgacg cccagcgggt
    11401 agtagtcgcc gggcgcgagg tcggcctccg cggccttcgc ctcggcggcc gcctcctggt
    11461 tcctgccccg aaggaggagg atgaaggcgc gcaacgcggc atggctgccg cggaggtagg
    11521 ccccctcctt tccgccgaac tggtcggcgt ccccggcggt cttggccatc caggactcgg
    11581 cctccggaat ccggccctgg aagaacctga cccaggcggc caggtcgtcg aggcgggccc
    11641 gcttgcggat ctcggcctcg ccgacggctt cgagatggcg gtcgagggtc gcgagctcgc
    11701 ccctgcggag gaagtcgatt ccttccagga ggcgggcggc ctcggccttg tttccggcgc
    11761 ggagctgaag ctcgagcgcc gtctccggat tcccgctcga ctcgtgccac gtggcggcct
    11821 tcgcgagcac ctccgcctcg acccccggcg cgagggcgcg aagccgggcc cgcagggtct
    11881 ccgcgaacag gtggtggtag cggtgccagc ggccctcgtc gtcgagcggc acgaggaaga
    11941 gccccgccgc ctcgatccgg ccgatcatcg ccgccgagcc gccggccggc ccgatcgccg
    12001 cgtcgcagag tcctgcgcaa aagcggtcca gcaccgcgct ccggaggagg aaggatcgcg
    12061 tctcctccga ttggaggccg aggacttctt cggtcaggta ctcgaggatg agtcggtcgc
    12121 tcccggagaa gcccctgatg aaggcctcga cgtcgggctt cctcccgagc gagatcgccg
    12181 cgagcctgag tccggcgccc cagccctccg tcttgtcggc gacctcgctg gcctgctccg
    12241 gactgagcct gcctcccgcg gcgccgtgga gcagggaggc cgcctcgtcg gggccgaagc
    12301 gcaggtcgtc ggcgcggagt tcggcgacga agccgccggc cctgagcctc gccagcggca
    12361 ggggtgggtc gcggcgggac aggaggagga tgcgcagccg ctctggcgag tgctccacga
    12421 agcgcgcgaa ggagtcgagg atctccttgg aggagatcag ctggaaatcg tccaggacga
    12481 ggaggaggaa gctgtcgagg gaatcgaccg cctcggcgag ggcggcggcg agctcgcggg
    12541 cgctcggggg ctggggagcc ctcgccgccg aaagcagggg cgcgaagggg gagatttccg
    12601 cggggtcagc cgcggcgagc tcgatggccg cggccgcggc gccccagaag cgcacagggt
    12661 cgtcatcctc gcgttccagg gagacccagg cggcgcgggg ctgcggtttc gcctgggccg
    12721 cctcggccgc gtcggacggg gcggcggcga gggcctccag gtactgcatc gccaaggagg
    12781 acttgccgaa gcccgcgggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    12901 ggcgagggcc tccaggtact gcatcgccaa ggaggacttg ccgaagcccg cgggggccga
    12961 gacaaggacg aggggcgagt ccaggcaggt tgctagcttc cgtagaagcc cgggccgggg
    13021 gagaagcccg cgcaatggct tgggaatcca tggcttagct cgaagaactt gatcggccac
    13081 gttctccagt atagcatggg taggagggcg ggcggcctaa gcgtcaggct tcggtgaatg
    13141 ccgcctggcg ccgcccgatc cgcggaaagc aaggcccgtc aggcgtccgg cggccaggtc
    13201 ttccccttat tctcccgttc gatgtccttg cggcgttacg tacggaagcc gccgttcggc
    13261 gcgcattttg agtccccaga gcatggcggg ctgctggaca gtcgcgccgg cgaggtcatt
    13321 catgaaccat gccgcggccc tggaggcgaa gtcctccccc atctggatgc gaaagcgcgc
    13381 aaggaggcgg ctccgcccct tgggaagcgg ttcgaccgcg aaagtccagg ccacgttgtt
    13441 cccgccgagg ctgtccggct cggcgacgag gacgaggaat cggggaacct ccgccatcgc
    13501 gatggtcatg ccaagggccg gcgcgaggtg gatcttgtcg ccttcgcggg cgctctggaa
    13561 ctcggggatg atcctcagcg ccgaagccct cgcgtcgtgg aagtacgccg gatcggccag
    13621 gcggttcacg aggtcgatgt tgtaccagcc cgcccgcttg tagccgatct ggaccagcca
    13681 cttccacacc tggtcgggag gggcgtcgat gtcgatgccc tgcgtatagc cggcggcgtc
    13741 tagcccgaca tactcgtccc cgggcatgcg cgccgcctct tcggaggagc ttgcgccgaa
    13801 gccgcgcatc gccggcccag cgacggcgag atagatgccg agggccaggc agcccaggac
    13861 ggcgaggccg agggtggcgc ggagcgaccg cctgaggcca agcggcagga gccgcggcag
    13921 gggcaggaag gcccgcgtcc tgcggcgata atcggcatag tcctggccga atagcgcgtg
    13981 gagttccctt tcctcggctt cggcggcctt gcccgccacg atccggaaga ggagggcgac
    14041 aggcaggaag agccaggaat cgagaagcag ggaaaaggag ggaatgaccg agaagatcca
    14101 ccaggaaaag ctgggattgc gcgagaggct gtaggcgccg ctggtcgcga ggcggcctgc
    14161 tctccaggcg cgcacgagct cgaaggcggc ggaggcctgg aacagcaacc cgagcccgag
    14221 aagctcgatg cccgcggcaa aggcgatgcc gcggagtttt ccccaggccg cgaaggcccc
    14281 ggggaatgcg acggtgagcg ccagggcggc gaagaaggcc ggcatgagcc tcagcgcgag
    14341 acggggcgcg tcgcccatga tcgacctggc ctcttttccc gcgacggtag gctggattct
    14401 caagttattc ctcctggagg gaaaccccgc gcggtcttgc gcgcgatccc tgcccaagtc
    14461 aggactatga gtctcgtccg cactcgtctt ctatcagtcg aaaggctgat tttcggaaac
    14521 cgggagccat cgccttcgcg tacatgagct tggggacccc gggcctgcgt cccgcagcgc
    14581 caccaggcgc cgggccgcgc agcgactcga gtctctacgt tctctcgtcc acatcaacgt
    14641 gggaggactt cgggatggga cgcgcgcgaa gcgccgcgcc ctcgcgcccg ggaggcctcc
    14701 gtcccgacgc gcgcgaagcg ccgcgtcgcc ccccgcagcc tcccgcctgc gcgaaaatag
    14761 ataaacgaga gggccgcccc cgcaagggag cggccctctc tgatttcgct tttcttcggc
    14821 cggcgggaaa gccgcgcaag tcggctttcc cgccgccgtc ttccctccgg cttaaccggc
    14881 gcgccgaagg cggcccggtt caagccggag cgtcagcgag cgccgttctt tagaacggcc
    14941 gctcgctgag gtatttgtac tccttccagc ggcgctcgac gaaggccttc tcggccgcga
    15001 ggagctcggc ggcgcgcttc tggtccgcgt cgcggagggt cttgaagcgc acctcggagt
    15061 acatgaactg ctcgatcttc gaggtgtcgg gctccttcgc gtcgagctgg aaggggttct
    15121 tgccctcggc cttgaggcgg gggtcgtagc ggtagagcgg ccagatgccc gcctccgcgg
    15181 agagcttctg ctggttctgg cccttcatca tgtcgatgcc gtgcatcgcg cagtgcgagt
    15241 aggcgatgat gatggagggg ccgtcgtagc tctccgcctc gcggaaggcc ttgatcgcct
    15301 gggccatgtt ggcgcccatc gcgatcgagg cgacgtagac gtagccgtag gacatcgcga
    15361 tcatgccgag gtccttcttg ccgatgtcct tgcccgccgc ggcgaacttg gcgaccgcgc
    15421 cgaagggcgt ggccttggag gcctggccgc cggtgttgga gtagacctcg gtgtccatga
    15481 cgaggaggtt cacgttgcgg ccggaggcga tgacgtggtc aaggccgccg aagccgatgt
    15541 cgtaggccca gccgtcgccg ccgaagatcc agacggactt gcggatcagg ttgtccgaga
    15601 cgttggcgag ctccttggcc cagccctcgg cggacttgcc gcaggcggcc ttgagctcgg
    15661 cgatccaggc gcgctgctgc gcgatgtcct cgtcggaggc gagcttgttg gaaaggagct
    15721 tgtcggccag gtccgcgggg actcccttgc ccttgttcgc ggagaggagc tcgcgggcgt
    15781 actcggcgag cttgtcggcg gagagccgca tgcccaggcc gaactccgcg gcgtcctcga
    15841 agaggctgtt ggaccaggcg gggccgcggc cgtcctcgcg gaaggcgtag ggcgtgttgg
    15901 ggaggttgcc gccgtagatc gaggagcagc ccgtggcgtt cgcgatgacc gcgcggtcgc
    15961 cgaagagctg ggtcatcagc ttgatgtagg gggtctcgcc gcagccggcg caggcgccgg
    16021 agaactcgaa gaggggctgc ttcatcgcga tgcccttcgg ggtcgcgagg ttcaggagct
    16081 tcgcgtccgt gttcgggatc ttgaggaagt gctcccagtt ggcggcctcg gcctcgcgga
    16141 gcgggagctg gggctcgagg ttgatcgcct tcctgccggc ggcggccttg tcggccacgg
    16201 ggcagatgtt cacgcaggcg ccgcagccgg tgcagtcctc cggggcgacc tggagggtgg
    16261 ccttgaggcc ggcgagctcc ttgcccttcg cgtcgacgga cttgaaggtc ttgggcgcgc
    16321 cttccgcgtg cttgggctcg taggccttga ggcggatgca ggcgtggggg cagaccatgg
    16381 tgcagttgcc gcactggatg cagatctcgg agttccagac ggggatcttc tccgcgatgt
    16441 tgcgcttctc gtacttcgtg gtgccggtgg ggaaggtgcc gtccatcggg agcttggaga
    16501 cggggagctt ctcgccgcgg gcggcgatca tctcgccgat gacctcgcgc acgaacttgg
    16561 gcgcggtcgc ggggacgggg agcttcatcg agtgcttgct cgtcgcctgg gcggggtact
    16621 tcacctcgtg ggtcgcgccg agggccaggt cgatggccgc gatgttcttg gcgacgacgt
    16681 cggcgccctt cctggcgtag gtcttctccg cgtacttctt gacgagcttc acggcctccg
    16741 cctcggggag gacgccggag atcttgaaga aggcggtctg catgacggtg ttgacgcggc
    16801 cgcccatgcc ggccttctcg gcgatctcca tgccgtcgat gacgtagaac ttgagcttct
    16861 tctcgatgat ccgcttctgc acctcgacgg ggatctcgtc ccagaccttg tcggcggaga
    16921 aggggctgtt gaggaggaag acgccgccgt ccttcgcgtt ggcgaggatg tcgagcttct
    16981 cgaggaagga gaacttgtgg caggcgatga agtcggcctt gccgatgagg taggacttct
    17041 tgatctgctt cggcccgaag cggaggtggg agatcgtgta ggttcccgcc ttcttcgagt
    17101 cgtagacgaa gtagccctgg gcggagttgt ccgtctcgtc gccgatgatc ttgatggagt
    17161 tcttgttcgc gccgacggtg ccgtcggagc cgaggccgta gaagaggcac tccttgacgc
    17221 cgtcgccctc gagctggaag ccctcgtcgt aggcgaggga ggtgcccgtc acgtcgtcgt
    17281 tgatgccgac cgtgaagtgg ttcttgggcg cgggcttggc gagctcgtcg aagacggcct
    17341 tcgcgtggca ggggtagaac tccgcggagc cgaggccgta gcggccgccg atgacggtcg
    17401 gccagccctt gaactgggcc ttgcccgagc cctgggcctc gccgatggcg gtgcggacgt
    17461 cctcgtacat gggctcgccg atggagccgg gctccttcgt gcggtccagg acggcgatgg
    17521 acttcacggt ggcggggagg gccttcatga agtgctcgac ggagaagggc ctgaagaggc
    17581 ggaccttcac ggcgccgacc ttctcgccct tcttgacgag gtggttcacg acctcctcca
    17641 cggtgtccgc gccggagccc atcacgatga cgacgcgctc ggcgtcgggg gcgcccacgt
    17701 actcgaagag cttgtagttc cggccgacga gcttgccgag cttctccatc tgcttctcga
    17761 cgatggcggg gacggcggcg tagagcttgt tcgcggcctc gcgggcggtg aagtagacgt
    17821 cggggttctg gctggtgccg cggatggagg gggtctcggg gttgaggccg cgggcgcggt
    17881 gggcgcgcac gagctcgtcg gagaccatct ggcgcatcac gtcgtaggac atctcctcga
    17941 ccttctggac ctcgtgggag gtgcggaagc cgtcgaagaa gtggaggaag gggacgcggg
    18001 cctcgagggt cgcggcgtgg gcgacgatgg ccatgtccat gacttcctgg acgttgttcg
    18061 cgccgagcat ggcccagccg gtctggcggg cggccatgac gtcctggtgg tcgc
//
LOCUS       DAOOSJ010000017        17819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2162_length_17819_cov_6.231705, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000017 DAOOSJ010000000
VERSION     DAOOSJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17819
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_17819_cov_6.231705"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLG14_01875"
     CDS             <1..752
                     /locus_tag="PLG14_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOX47906.1"
                     /translation="SDGSFVSLRAFALPYADYRGAFADNPYLIRVTQKPRARPPAPPP
                     VPAAPAPPPPRKPDPDLYMPETVRAFEGLAEAGKGEARFSEGEADIVPVLSSLLDRAK
                     GKSVDLVVCLDTTDSMKDDIDAVKRAIPEMLKARLAEFSSFRLGFVLYKDYFEDYLVK
                     KFDFTREFSVFTSELSAVRVAGGRDIPEAVYEALYTALVEYPWAAERKMIVLIGDAPP
                     HPLPRGTVDRALVEATAARLGVEMDAIILPH"
     gene            852..1790
                     /locus_tag="PLG14_01880"
     CDS             852..1790
                     /locus_tag="PLG14_01880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX47907.1"
                     /translation="MQRKRKADRIDPIARLATGRNFLLSASFLAGLFILVGSPARLVD
                     PAERAVGGMALARPELLEASSEGEVADRGLFFSSYRVSPGDTVGAIAERFGVSQDAII
                     SFNDIQNTRALRPGQLLKVPSMAGILYTAKEGDSVESVAAAFEIDADGIIEANRLLKP
                     ELPATKVVFLPDARLPSFKLREINGDLFRWPVRGWVTSRFGWRNDPFSGSRSFHNGLD
                     IGAGMGTPILAAMEGRVVETGYSSTLGNYVLLSHHSGWSSFYGHMSSVSVKAGQYVNL
                     GSKIGAVGSTGYSTGPHVHFSVFKYGRSMNPSVVLH"
     gene            1808..3124
                     /locus_tag="PLG14_01885"
     CDS             1808..3124
                     /locus_tag="PLG14_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOX47908.1"
                     /translation="MTSTQRLYYSRPGLAEAEALVLSAEGGALVLDRTIFYPEGGGQP
                     CDLGTIGGIPLEGVELSDGLVLHRLALPPPAGLSVPGPGDRVVLRLDAARRLDHAEQH
                     TGQHLLSAVCSRLYGAETKGFHLGREASTIDLELGSGAFLGPEELAEAEARAAEAIAE
                     DYPVLVHLCPPEDLASFPLRKGPPEGEEEIRVVEIDGIDYSPCCGTHLPGAGRIRVLK
                     IVGAERRKGMTRVSFLAGGRAVRDYGEALAREREAARLLGAAREELPEAAARAASRLR
                     ESLKEAEALRGERAALEAELALRRSGGPASVLAFRFPDRDAAAAELAAKAAAALGRPG
                     LAASAPDLTCVAALPQPGAANAAASSRPAGGAGIGAGAAPGAGAQHGAANASAAGLAA
                     LLKPLAAASGGRGGGGAAHFRAVFPDLGALDSFFQAAFERIGGINR"
     gene            3180..3965
                     /locus_tag="PLG14_01890"
     CDS             3180..3965
                     /locus_tag="PLG14_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX47909.1"
                     /translation="MDKDRIGAQRLFELAARSQLRIRKALEAALRGLGLTYAQYGALQ
                     ALDAKEGLSQSELALALETDATTAMVLRHSLEKKGLIERAGDPSDGRVKRIALTQAGV
                     SAFSSARPAVEAVYAAADGLVSEQEGKRAAALLERLALFAGEAAQAASPKGQAPAEGA
                     RRRGRPRKNPVETEPKEPKRRGRPRKDEAAKAGAAKAAEGKAKRGRKKAAAQDGLAEA
                     QASSEAAGKTSSRKAPKAGAKAPKAPAKKAAKPAAKPRKARDS"
     gene            complement(4130..5470)
                     /locus_tag="PLG14_01895"
     CDS             complement(4130..5470)
                     /locus_tag="PLG14_01895"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOX47910.1"
                     /translation="MRTMGDLESRFEGVSGALLGGLKAGEALSLEFAGEASAFMRFSG
                     GRVRQTGEVEAAVVEFRLYAGGRTLRKAFSLPGNAMGDLAAAALALKEARGEAARLPP
                     DPYQVLPAAAERSSEAYPGSLPDPEELPREILGPAEGLDFVGLHAQGRVARGAANSSG
                     ARHFFATENFSTDWSAWLPSGKAVKSVYAGRDWDGEEHARRIAASRPLLEGLSLPERR
                     LEPGSYRAYLSPEALGALMVFFSWRGLSERELREGESSFLALREGRRSLSPKFTLVQD
                     FSLGVEPRFNGLGETAPERLVLVDRGRLAASLVSARTARQYGLASNAAPEDESLRSPA
                     VAPGGLPAAEALQALDTGLYVSNLHYANWSDFASARVTGMTRFACFWVEGGKIAAPIK
                     DMRFDESLYELLGGGLLDLTAERSLVPSTGSYGLRDVGGRLLPGLLLGGLRLTL"
     gene            complement(5467..6906)
                     /locus_tag="PLG14_01900"
     CDS             complement(5467..6906)
                     /locus_tag="PLG14_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOX47911.1"
                     /translation="MTSDLTRLLQAAIPPGAEWTGLRRVRSVSRSYSARDGRFDEAFS
                     EEDSGFMVEVLSGGQFGYAATADGSPSGLREAGLRALTLARAAGPYAIHRFGAGQRPS
                     WKVRWESPRAKSGSLGADILAEAAIAVSAAMRIDRRIVQTSASLETRDLEIELVSSSG
                     ASLAQVLHLAGHGRKATARSGDVVQARTGHGPRGELRQAGAEFLDLPALLEAAARTAR
                     EALELAEAEPCPAGKADLLLAPDQMALQLHESVGHPLELDRILGDERNFAGSTFVRPE
                     DIGRLRYGSELMNVSFDPSLAGEAASFAFDDSGAPAERVLLVEKGLLVRALGGLESQA
                     RSGKPGAACQRADGWRRPPIDRMANLNLEPGESSMEEMVASIERGVLMEANRSWSIDD
                     RREKFQFGCEYGRLIKDGRLCKVVRDPNYRGVSSSFWRSLFKVGDRSTFAAFGTPNCG
                     KGEPNQLQFVGHASPACAFRGVEIFGGEA"
     assembly_gap    7038..7137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7238..8368)
                     /locus_tag="PLG14_01905"
     CDS             complement(7238..8368)
                     /locus_tag="PLG14_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOX47912.1"
                     /translation="MDDMDDGQEGGAELLKNLGFPRIRMHHPFNHFDFAKAGRRILVI
                     GPMGSGKTEYSARIWRDGLVARKKGSGAAAATSQAGADRRDIFVVRSSLDAARFPDYP
                     ADALAYRGGYERCGSRVGQARDSFALESLIAENPEAGTWIVDEAAFYDERVAYLMKDE
                     SERRGLTFVCPTLVLNFRREIFNQTARLLLDTATDVFPLTAYCEHPDCLADSLYTYRY
                     YTVEGLECPALYFDPLIIIGGDRTKEDGREPNYCTRCDAHHYLPGKEYSYFTLKPLGE
                     AAARGDLKPLLAELNAIAGAIESSRLYASLRSRYLECAEPCPTQLNALRVPCIAERAL
                     VYLYAEQNLLSAEQLKSVARALGLDRDFLMRRLSDNRRPLEL"
     gene            complement(8388..9362)
                     /locus_tag="PLG14_01910"
     CDS             complement(8388..9362)
                     /locus_tag="PLG14_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47913.1"
                     /translation="MRKGFSTVLYLFIGLPLTLSALLCLSMKPWVLDRDFYKRFATDD
                     RLYAALRSPEAAKGVEARLEVEGAVFSGPELVAAFQPRIPEAELKALGSSAVDQVFDA
                     LESRGRAESLLLDLKPLKAALSKSAPSAAADYAKAIPVRAEKAEAGDFSFRPEGSSVE
                     ATARKVGAAFQAAAAQIPDQAEEKELRVDAGLPMGRSLKAAFDSTTLTLSLLSALLVA
                     GLALMAGGGAGAVLSRAGSYLIPPSVLVLVPGILLSLPGNAIFGRIGTLPPEAASFLS
                     SEAGASLAAYLRAVLGQMSRGFFVTGLVGVSVGAALKALRRAFEPSEI"
     gene            complement(9451..11145)
                     /locus_tag="PLG14_01915"
     CDS             complement(9451..11145)
                     /locus_tag="PLG14_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX47914.1"
                     /translation="MSRQHGMAAALLALALAAGAASAAEAGAAPAAAPAELSVAFNAL
                     ELQFDPQHSIYSAEAQIFTGVYEGLFSYDPSNLDPVKAACRSFSRSRDGLTYTFYLRD
                     EAKWSDGSPLLARDFRDAWLRALSPAEKADYAAFFDVIAGAKDYRLGKTKDPASVGVS
                     VIDDRVLQVRLAAPAAYFTRLLCHHSFSPVHPSMLKTRDWEKAFPFPVNGPYKPASYK
                     KGEPGELLLVRNPLYWDASSIRIDKVRVVLTDDDAEVTRLYDNGEMQWLAGPMDLDAL
                     LNRSAIQVSPMFGTQYWFFDCSGEPWRSADLRRGLALLLPWKEIRSRDSYLMPAKTLV
                     LPFDGYEKAKGIEETDEEAGRALLAKAGYPDGKGLPPLMILVPADGEDAARVAALMEA
                     AWERLPGLAVELKKVPSASYFSVVRAGPAKGGYTMALTTWIGDFADPLAFLGMWTADS
                     NLNDSGYTDPEFDALLAASASKEGDARLDVLAEAETLLLSGAAVLPTYHSIAVNVVDS
                     DYLEGWFSNALDLHPFKYLGFGERRVRPNVARLPGAAAAAARPSRAGETELVAALH"
     gene            complement(11226..12641)
                     /locus_tag="PLG14_01920"
     CDS             complement(11226..12641)
                     /locus_tag="PLG14_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47915.1"
                     /translation="MEEAQAFAKSLEERLDAKRDSLDRDELPKMKETWKLFQTAFQGL
                     YGVLFKKGIVHEDPYKYELKISEVTTPPEGPFAESEKLDQMSLRLSQFESYLDFLNNY
                     YQFSVDFLTMGRIKRLLGLVKYFNFTQFSDTSNQLNTRCLAELVGMVKRGSDQLSSGL
                     IAESASQLDKASRDILAALKELASYHRERYKLELRQLVMPSLSLDSAQAITHRDEALR
                     QVRRKFSELAGDRPFYAELAEEVLMEDHSSDGPQLRDELLRRLEVKSEKKTEKAKEKS
                     YKGVVLDAVRILAGINYPLEDAVQKMSDNSSVLESRNTGFLQRLKAAIRNVFSPQDKG
                     IAYEVEILDAVTGTRTTESIDIAAFVEEGTKKAKQLAGLLQRGGPGWRRLETAPEELS
                     FKFLERTMEELQRLVKRIAALDEYFKSATPAEDRPKLRGAKAEITTIKGALIKANQKK
                     HEYVAQKEELEQMRRLGIRAE"
     assembly_gap    12759..12858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12901..13689
                     /locus_tag="PLG14_01925"
     CDS             12901..13689
                     /locus_tag="PLG14_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47916.1"
                     /translation="MRAIVRRPRRRRLVPAFIPLLACLAAASACCLPSGGQPFGPLAA
                     ERLLLELPAAPEAWTGLPELSFRLSWRDEEGRFRSAWLDAGFRLEARVRRGLPQALLA
                     EPYSLGRPLRPAGALYPAGLGTEGRLGLDWRGGWAASVFRTLEEGGEDPAAYDFGRLS
                     RELEARGGDPWATLAPLAAAGLLASGRFRADRLDPPTRFPVPLPGPGPWWPESPLAAA
                     PAPDSEGGGWAVGLEPGFHRYYGSGRLLSAQVEATGEAMVLVSP"
     gene            complement(13761..15812)
                     /locus_tag="PLG14_01930"
     CDS             complement(13761..15812)
                     /locus_tag="PLG14_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX47917.1"
                     /translation="MAAGIFGQSLRSIAAAALAATVAVAGAALVVMDGLYGDANAKAL
                     GEAARSLAAALPDEALPALAPDAAGGERAAARAAAARFAARTAGGSSFRVTLIALDGG
                     VAADSKADPAAMENHSGRPEVEAALRGGVGTARRRSATVGMELFYAAAPVLRDGRVAG
                     ALRVAADLPALESRLGPARLALAAAALLAGLVSLAAAAYYSRRASRPIARLAEAAKAY
                     AAGPAAAVLARPEAAAEAAIGRSAGPAAASHEAAATEVAAQDQPAAAALGAPEGRPLA
                     SSFRVREGPEEIRVLGRCLEAMAAELAARVAEAEAEGRERRAILDGMAEALLSLDGRL
                     RVRLANPAAAALFGLGSPAEAVGKSLLEASRSTELEAAAREALASGSRLEAEIALYSG
                     GERWFQAVAAPFPKPGASPAPGGREESGPGGAGAAEGLILVLSDITRLRRLERIRRDF
                     VANVSHELRTPVQLVKGFAENLRGGSLADPGKADRFLAIIERNAARMENLIDDLLVLA
                     RLEQEGQGGLPREDTELAPLLEAAREAVLPRAEAKGIAIRLDCAEGLKAGLNGGLVEQ
                     AVVNLLDNAVKYSPSGKSVELRARSEAGGPASDRLRIEVRDRGIGIPAKDLPRVFERF
                     YRVDKARSREQGGTGLGLAIVRHVALAHGGSVSVESWEGEGSLFTILIPLGGAAAGKG
                     A"
     gene            complement(15815..16516)
                     /locus_tag="PLG14_01935"
     CDS             complement(15815..16516)
                     /locus_tag="PLG14_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX47918.1"
                     /translation="MAKAKVLIVEDDPDIRELLAASLAQEGWILDLAPDGEEGLARLE
                     KERPDCVVLDIMLPGIDGLEVLRRIRAEPSRRRLPVLMTTARGEDSDVVAGLELGADD
                     YVVKPYSPKVLAARIRAALRRGAELAEQATAGATRVKRGGLTVDSARHQAEFGGKRIE
                     LSATEFAILDFLMRSPGWVFSRSQIIDAVRGRDYPVTDRSVDVQILSLRKKLGPGGEL
                     IETVRGVGYRLRDGD"
     gene            complement(16526..17185)
                     /gene="phoU"
                     /locus_tag="PLG14_01940"
     CDS             complement(16526..17185)
                     /gene="phoU"
                     /locus_tag="PLG14_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOX47919.1"
                     /translation="MGNRIQLMDEMNRLHHDLLSMGTRVEEDLRKAIEALKRQDVELA
                     KEVKADDAVVNALQLKIEDQTAVLIATQAPVARDLRELVALLKVTDNLERAGDHAVHL
                     AKAAIKLSGEPPFRQIERLERMASVGCDMVRGAVGAYLNRDVEEARRVAALDDGIDGE
                     HRALVQEVLELMKERPDQVQRAARLLTTSGFLERLGDHMTNVCEAVVYMVEGSHVELN
                     D"
     gene            complement(17205..>17819)
                     /locus_tag="PLG14_01945"
     CDS             complement(17205..>17819)
                     /locus_tag="PLG14_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOX47920.1"
                     /translation="ALRAFNRMNELIPGTKTGGEVLFHGINVYDPGVDPVQVRRWIGM
                     VFQKPNPFPKTIHENIAWGARINGFSGDMDELVESSLRQVALWDEVKDKLRQSAFALS
                     GGQQQRLCIARAIAVRPEIILMDEPTSALDPISTSKIEDLLDELKKRYTIIIVTHNMQ
                     QAGRVSDSTAFLLHGELVEYAPTKELFFNPKDERTENYLSGRFG"
BASE COUNT         2414 a         6108 c         6518 g         2579 t
ORIGIN      
        1 tctccgacgg cagcttcgtg agcctccgcg ccttcgccct gccctacgcc gactatcgcg
       61 gcgccttcgc ggacaatcct tacctgatcc gcgtcaccca aaagcccagg gcccggccgc
      121 cggctccccc tcccgtcccg gccgcccccg cccctccgcc gcctaggaag cccgaccccg
      181 acctctacat gccggagacc gtccgcgcct tcgaggggct ggccgaggcg gggaagggcg
      241 aggcccgctt ctccgagggc gaggcggaca tcgtgcccgt cctctcgagc ctcctcgacc
      301 gcgcgaaggg caagagcgtc gacctcgtcg tctgcctgga cacgacggac tccatgaagg
      361 acgacatcga cgccgtgaag cgggccatcc ccgagatgct caaggcccgg ctcgcggaat
      421 tctcctcctt ccgcctgggc ttcgtcctct acaaggacta cttcgaggac tacctcgtga
      481 agaaattcga ctttacgcgc gagttctccg tcttcacttc cgagctctcc gccgtcaggg
      541 tggcgggggg gcgggacata ccggaggcgg tctacgaggc cctctacacg gccctcgtcg
      601 agtatccctg ggccgccgag cggaagatga tcgtcctcat cggcgacgcc ccgccccacc
      661 ctctgccccg cggaacggtg gaccgggccc tcgtggaggc gacggcggcg cgcctgggcg
      721 tggagatgga cgccatcatc ctgccccact agggcgggcg gacgcctccc tgcgacattt
      781 ttccttgcgt cgccctgggc atccgttgta aagttggttt cgtatacctc ttgaaaggat
      841 ccaaagagag catgcagagg aagcggaaag ccgacaggat cgatcccatc gcccgcctgg
      901 cgaccggaag gaatttcctc ctttccgcct ccttcctcgc ggggctcttc atcctcgtgg
      961 gctcccctgc caggctcgtc gacccggcgg agagggcggt cggcggaatg gccctggccc
     1021 ggcccgagct cctggaggcc tcctccgagg gcgaggtggc cgaccggggc ctcttcttct
     1081 cctcctaccg cgtctctccg ggcgacaccg tgggcgccat cgccgagcgc ttcggcgtga
     1141 gccaggacgc gatcatcagc ttcaacgaca tccagaacac gagggccctc cgccccggcc
     1201 agctcctcaa ggtgccgagc atggcgggca tcctctacac ggccaaggag ggcgactcgg
     1261 tcgagtccgt cgccgccgcc ttcgagatcg acgcggacgg catcatcgag gccaaccgcc
     1321 tcctcaagcc cgagcttccg gccaccaagg tcgtcttcct ccccgacgcc cgcctcccga
     1381 gcttcaaact ccgcgagatc aacggcgacc tcttccgctg gcccgtccgg ggctgggtga
     1441 cgagccgttt cggctggcgc aacgatccct tctccgggag ccgctccttc cacaacggcc
     1501 tcgacatcgg cgcgggcatg ggcacgccca tcctcgccgc gatggagggc cgggtggtcg
     1561 agacgggcta ctcctccacc ctcgggaact acgtcctcct ctcccaccac tcgggctggt
     1621 cgagcttcta cggccacatg agctccgtct ccgtcaaggc cggccagtac gtgaacctgg
     1681 gctccaagat cggcgccgtc ggctcgacgg gctattccac gggcccccac gtgcatttct
     1741 ccgtcttcaa gtacggacgc tcgatgaatc cctccgtcgt cctccactga cgcgctatac
     1801 tgggggcatg accagcaccc aaaggctcta ttactcgcgg ccaggcctgg ccgaggccga
     1861 ggccctcgtg ctctccgccg agggcggagc cctcgtcctc gaccggacga tcttctatcc
     1921 cgagggaggc ggccagccct gcgacctcgg gacgataggc ggcatccccc ttgagggggt
     1981 cgagctctcc gacggcctcg tcctccaccg cctggccctg ccgcctcccg ccggcctctc
     2041 cgtccccggc ccgggcgacc gggtcgtcct gcggctcgac gcggcgcggc gcctggacca
     2101 tgccgagcag cacacgggcc aacacctcct ctcggccgtc tgctcccgcc tctacggcgc
     2161 cgagaccaag ggcttccacc tgggccggga ggcctcgacc atcgacctgg aactcggctc
     2221 gggcgccttc ctcggccccg aggagctggc cgaggccgag gcgcgggccg cggaggccat
     2281 cgccgaggac tatcccgtcc tcgtccacct ctgcccgccc gaggacctgg cctccttccc
     2341 cctgcgcaag ggccctccgg aaggcgagga ggagatccgc gtcgtcgaga tagacggcat
     2401 cgactacagc ccctgctgcg gcacccacct tccgggcgcg ggaaggatcc gcgtcctcaa
     2461 gatcgtcggc gcggaaaggc ggaagggcat gacgcgcgtc tccttcctgg cgggaggaag
     2521 ggcggtccgc gactacggcg aggccctggc ccgcgagagg gaggcagccc gcctcctcgg
     2581 agccgcgcgc gaggagctcc ccgaggccgc ggccagggcg gcatcccggc tcagggagag
     2641 cctcaaggag gccgaggccc tccgcgggga gcgggcggcc ctggaggccg aactcgccct
     2701 ccggcgctcc ggaggcccgg cctcggtcct ggccttccgc ttccccgacc gggacgcggc
     2761 cgcggccgag ctcgcggcca aggccgccgc ggccctgggc cgccccggcc tagcggcctc
     2821 ggcgccggac ctgacctgcg tcgcggccct gccccagcct ggagcggcca acgcggcggc
     2881 ctcgtcgcgg ccggccggcg gggcgggcat cggtgcgggc gccgcgccgg gagccggagc
     2941 ccagcacgga gcggccaacg cgtccgccgc gggcctggcc gccctcctca agcccctggc
     3001 ggcggcctcg ggcggccgcg gaggcggcgg cgcggcccat ttccgggccg tgttcccgga
     3061 ccttggggcc ctggactcgt ttttccaggc cgcctttgag cgaattggcg gcattaatcg
     3121 ttgacaattg aaaaattacg aaatagtatt tatatcaagt taattgtaag gagcttccca
     3181 tggacaagga caggatcggc gcccagcgcc ttttcgagct ggcggccagg tcccagctgc
     3241 gcatacggaa ggccctggag gccgccttga ggggcctggg cctcacctac gcccagtacg
     3301 gcgccctcca ggccctggac gcgaaggagg ggctctccca gtcggagctg gccctggcgc
     3361 tggagacgga cgcgaccacg gcgatggtct tgcgccactc cctggagaaa aagggcctca
     3421 tcgagcgggc gggcgatccc tccgacggcc gagtcaagcg gatcgcgctg actcaggcgg
     3481 gcgtctcggc cttctcttcg gccaggcccg cggtcgaggc tgtctacgcc gcggccgacg
     3541 gcctggtctc cgagcaggag ggcaagaggg cggccgccct actggagcgc ctggcccttt
     3601 tcgcgggaga ggcggcgcag gccgcctccc ccaagggcca ggcgcccgcc gagggcgcga
     3661 gacgccgcgg gcgtccccgg aagaatcccg tggagaccga gccgaaggag cccaagcgcc
     3721 gcggccgccc ccgcaaggac gaggccgcga aggccggcgc cgccaaggcc gcggaaggga
     3781 aggccaagcg cggaagaaag aaggccgcgg cccaggatgg cctggccgag gcgcaggcct
     3841 cctccgaggc cgccggcaag acctcctcca ggaaggcccc caaggcgggc gcgaaggcgc
     3901 ccaaggctcc ggccaagaag gccgcgaagc ccgcggccaa gccccgcaag gcccgcgact
     3961 cctaggaaaa agaatgtaga ggaaggggcc gcccgccggg cggccccttc gcgtctcagg
     4021 cggccttcct ccgcgccccg gccccgcgcc ttaggcccgc ggcccgggca aaggccttcg
     4081 cccaggcccg ccccctcttc gggcctcgcc gggccgggcc aagccggccc tagagcgtca
     4141 gcctcaggcc gcccaggagg aggccgggca ggagccgccc ccctacgtcg cgcaacccgt
     4201 agctcccggt cgagggcacg aggctccgct cggccgtcag gtcgaggagg cccccgccca
     4261 ggagctcgta gaggctctcg tcgaagcgca tgtccttgat cggggcggct atcttccctc
     4321 cctcgaccca gaagcaggcg aagcgggtca tgccggtgac gcgggccgag gcgaagtcgc
     4381 tccagttcgc gtagtggagg ttcgagacgt agagtcccgt gtcgagggcc tgcagggcct
     4441 ccgcggcggg gaggccgccc ggggcgacgg cgggcgagcg caggctctcg tcctcggggg
     4501 cggcgttgga ggccaggccg tattggcggg ccgtcctggc ggagacgagg ctggcggcga
     4561 gcctgccccg gtcgacaagg acgaggcgct cgggcgccgt ctcccccagg ccgttgaagc
     4621 ggggctcgac gcccaggctg aagtcctgga ccagggtgaa tttcggggag aggctccgcc
     4681 tgccctcgcg gagcgcgagg aaggagctct cgccctccct gagctcgcgc tccgagaggc
     4741 ccctccagga gaagaagacc atgagcgcgc ccagggcctc gggggacagg taggcccggt
     4801 aggagcccgg ctcgagcctg cgctcgggca gggagaggcc ctcgaggagg ggccgcgacg
     4861 ccgcgatgcg cctcgcgtgc tcctccccgt cccagtcgcg gccggcgtag acggacttca
     4921 cggccttgcc cgaggggagc caggccgacc agtcggtcga gaaattctcc gtggcgaaga
     4981 aatgccgcgc gcccgaggag ttggccgctc ccctcgcgac ccggccctgg gcgtggaggc
     5041 cgacgaagtc caggccctcg gcgggcccga ggatctcccg gggcagctcc tcgggatcgg
     5101 gcaggctgcc gggataggcc tccgaggagc gctccgcggc ggcgggcagg acctggtagg
     5161 ggtcgggagg gaggcgggcc gcctcgcccc gggcctcctt cagggccagg gccgcggccg
     5221 cgaggtcgcc catcgcgttg ccggggaggg agaaggcctt gcgcagggtc cgcccgccgg
     5281 cgtagaggcg gaattcgacg acggccgcct cgacctctcc cgtctggcgg acccgcccgc
     5341 ccgagaagcg catgaaggcc gaggcctcgc cggcgaactc gaggctcagg gcctcgccgg
     5401 ccttgaggcc gcccaggagg gcgcccgaga ccccctcgaa ccggctttcc aggtcgccca
     5461 tcgtcctcat gcctcgcctc cgaagatctc cacgccgcgg aaggcgcagg ccgggctcgc
     5521 gtgccccacg aactggagct ggttcggctc gcccttgccg cagttgggcg tcccaaaggc
     5581 cgcgaaggtg gagcggtcgc cgactttgaa gaggcttcgc cagaaggagg atgagacgcc
     5641 ccggtagttg gggtcgcgga cgaccttgca gagccggccg tcctttatga ggcggccgta
     5701 ctcgcagccg aactggaatt tttcgcggcg gtcgtcgatg gaccaggagc ggttggcctc
     5761 catgaggacg ccccgctcga tggaggcgac catctcctcc atgctcgact cccccggctc
     5821 caggttgagg ttggccatgc ggtcgatcgg cggcctcctc cagccgtcgg cgcgctggca
     5881 ggccgcgccg ggcttgccgg agcgggcctg gctctcgagg ccgcccaggg cccgcacgag
     5941 gaggcccttc tccacgagaa ggacccgctc ggcgggcgcg cccgagtcgt cgaaggcgaa
     6001 ggaggctgcc tcgccagcga ggctggggtc gaagctcacg ttcatgagct cggagccgta
     6061 gcgcagcctc ccgatgtcct ccgggcggac gaaggtcgag cccgcgaagt tgcgctcgtc
     6121 cccgaggatg cggtcgagct ccaggggatg gccgacgctc tcgtggagct ggagcgccat
     6181 ctggtcgggg gcgaggagga ggtctgcctt ccccgcgggg cagggctcgg cctcggcgag
     6241 ctccagggcc tcgcgggccg tccgcgccgc cgcctcgagg agggccggaa ggtccaggaa
     6301 ctccgcgccg gcctgccgga gctcccccct ggggccgtgg ccggtcctgg cttggacgac
     6361 gtcgcccgag cgtgccgtcg ccttgcggcc gtggccggcc aggtggagga cctgggccag
     6421 ggaggccccc gagctcgaga ccagctcgat ctcgaggtcg cgggtctcga ggctggcgct
     6481 tgtctggacg atccggcggt cgatcctcat ggcggcggag acggcgatcg ccgcctccgc
     6541 gaggatgtcc gcgcccaagg agccgctctt cgcgcgggga gactcccagc ggaccttcca
     6601 ggagggcctc tgtcccgcgc cgaagcggtg gatggcgtag gggcccgccg ccctcgcgag
     6661 ggtcagggcc ctgagtccgg cctccctgag cccggagggg ctcccgtccg ccgtggctgc
     6721 gtagccgaac tggccgcctg agaggacctc caccatgaag cccgagtcct cctcggagaa
     6781 ggcctcgtcg aagcggccgt cgcgggccga gtagcttcgc gagacggagc gtacgcggcg
     6841 caggccggtc cattcggccc cgggcgggat cgcggcttgg aggagccgcg tgaggtcgct
     6901 cgtcatgaaa aactcctagt ttaaatggga aacttaccta ttatagcatg gttctctgga
     6961 gaaagaaggg aaaagacgag gatagctttt ctcgggcagg cccgcgccac ggccggaccg
     7021 gggagccgag cttgaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg
     7141 cctggctgcg ggctccggcc tggcggcgag gggtggccgc cggcctcttt ccgcagccag
     7201 ccgcgccagc cgcgcgccag ccgcaggccg cgccggccta gagctcgagg gggcggcggt
     7261 tgtcggagag ccgcctcatg aggaagtcgc ggtccaggcc cagcgcccgg gccacggact
     7321 tgagctgctc ggcggagagg aggttctgct cggcgtagag gtagacgagg gcccgctccg
     7381 cgatgcaggg cacgcggagc gcgttcagct gcgtggggca gggctcggcg cactcgaggt
     7441 agcgcgagcg cagcgaggcg tagagcctgg agctttcgat ggcgccggct atcgcgttga
     7501 gctcggccag gaggggcttg aggtcgcccc gggctgcggc ctccccgagg ggcttcaggg
     7561 tgaagtagct gtactccttg cccgggaggt agtggtgggc gtcgcagcgg gtgcagtagt
     7621 tgggctcgcg cccgtcctcc ttcgtcctgt cccccccgat gatgatgagg gggtcgaagt
     7681 agagggcggg gcactcgagg ccttccaccg tgtagtaccg gtaggtgtag agggagtcgg
     7741 ccaggcagtc ggggtgctcg cagtaggcgg tgagggggaa gacgtccgtc gccgtgtcca
     7801 ggaggagcct cgcggtctgg ttgaagatct cgcggcggaa gttgaggacg agggtggggc
     7861 agacgaaggt gaggccgcgg cgctcggact cgtccttcat gaggtaggcg acccgctcgt
     7921 cgtagaaggc cgcctcgtcg acgatccagg tgcccgcctc cgggttctcc gcgatgaggg
     7981 actcgagggc gaaggagtcg cgggcctggc cgacccgcga gccgcagcgc tcgtagccgc
     8041 cgcggtaggc cagggcgtcc gccggatagt cggggaagcg ggcggcgtcc aggctggagc
     8101 ggacgacgaa gatgtcgcgc cggtccgccc ccgcctggct cgtcgcggcc gcggcgcccg
     8161 agcccttctt ccgcgcgacc aggccgtcgc gccagatccg cgccgagtac tcggtcttgc
     8221 ccgagcccat gggcccgatg acgaggatgc gcctcccggc cttcgcgaag tcgaagtggt
     8281 tgaagggatg gtgcatcctg atgcggggga agccgaggtt cttgaggagc tcggcgcccc
     8341 cttcctggcc gtcgtccatg tcgtccatcc ctccgagccc ccgcgcccta gatctcgctc
     8401 ggctcgaagg cgcggcggag ggccttgagg gccgcgccga cggagacgcc gaccaggccc
     8461 gtgacgaaga agccgcgcga catctggccc aggacggccc tgaggtaggc ggccaggctc
     8521 gcgccggcct cgctggagag gaaggaggcg gcctcggggg ggagggtccc gatccgaccg
     8581 aagatggcgt tcccgggcag ggagaggagg atgcccggca cgaggacgag gacggagggc
     8641 gggatcaggt aggagcccgc gcgggagagg acggcgcccg cgccgccccc cgccatcagg
     8701 gccaggccgg cgacgaggag ggcggagagg aggctcaagg tgagggtcgt ggagtcgaag
     8761 gccgccttga gggacctgcc catggggagg ccggcgtcga cgcggagctc cttctcctcg
     8821 gcctggtcgg ggatctgggc ggccgccgcc tggaaggccg cgccgacctt cctcgccgtg
     8881 gcctcgacgg agctgccctc gggcctgaag ctgaagtcgc ccgcctcggc cttctccgcc
     8941 cggacgggga tggccttcgc gtagtcggcc gcggcggagg gagcggactt ggagagggcg
     9001 gccttgaggg gcttcaggtc caggaggagg ctctcggccc ggccccgcga ctccagggcg
     9061 tcgaagacct ggtcgacggc cgagctcccc agggccttga gctccgcctc ggggatgcgg
     9121 ggctggaagg cggccacgag ttcggggccg gagaagacgg cgccttcgac ctccaggcgg
     9181 gcctcgacgc ccttggcggc ctccggggag cggagggcgg cgtagaggcg gtcgtccgtg
     9241 gcgaagcgct tgtagaagtc ccggtcgagg acccagggct tcatcgagag acagaggagg
     9301 gccgagaggg tgaggggaag gccgatgaag aggtagagga cggtcgagaa tcccttgcgc
     9361 atgctggctc cttcgggact acgggcaggg acgccgccgt gggccgccag ctaggggcgc
     9421 gcctcgcgcg gctccgggaa aaggacagct tcagtggagg gccgcgacca actccgtttc
     9481 gccggcgcgg gagggtcgcg ccgcggcggc ggccgctccg gggagccggg cgacgttggg
     9541 cctgacccgc cgctcgccga agccgaggta cttgaagggg tggaggtcga gggcgttgga
     9601 gaaccagcct tcgaggtagt ccgagtcgac gacgttgacc gctatgctgt ggtaggtggg
     9661 caggacggcg gcgccggaga ggaggagggt ctccgcctcc gccaggacgt cgagccgcgc
     9721 gtcgccctcc ttggaggccg aggcggcgag cagggcgtcg aattcggggt ccgtgtagcc
     9781 cgagtcgttc aggttcgagt cggccgtcca catgcccagg aaggcgagcg ggtcggcgaa
     9841 gtcgccgatc caggtggtga gggccatggt gtagccgccc ttcgcggggc ctgcgcggac
     9901 gacggagaag taggaggccg agggcacctt cttgagctcg accgcgaggc cggggagcct
     9961 ctcccaggcg gcctccatca gggcggcgac gcgcgccgcg tcctcgccgt cggcggggac
    10021 gaggatcatg aggggcggga ggcccttgcc gtcgggatag ccggccttgg cgaggagggc
    10081 gcggccggcc tcctcgtccg tctcctcgat gcccttcgcc ttctcgtagc cgtcgaaggg
    10141 gaggaccagg gtcttcgcgg gcatgaggta ggagtcccgg gagcggatct ccttccaggg
    10201 aaggaggagg gccaggcccc gccttaggtc ggcgctcctc cagggctcgc cggagcagtc
    10261 gaagaaccag tactgggtgc cgaacatcgg agagacctgg atcgcggagc ggttgaggag
    10321 ggcgtccagg tccatcgggc ccgcgagcca ctgcatctcg ccgttgtcgt agagccgcgt
    10381 gacctcggcg tcgtcgtcgg tgaggacgac cctcaccttg tcgatcctga tggaggaggc
    10441 gtcccagtaa aggggattgc gcacgaggag gagctcgccc ggctcgccct ttttatagga
    10501 ggcgggcttg taggggccgt tgacggggaa ggggaaggcc ttctcccagt ccctcgtctt
    10561 gagcatcgag gggtggaccg gcgagaagga gtggtggcag aggaggcggg tgaaataggc
    10621 cgcgggcgcc gcgagcctga cctggagcac ccggtcgtcg atcacgctga cgccgacgga
    10681 ggcgggatcc ttcgtcttcc cgaggcggta gtccttggcc ccggcgatga cgtcgaagaa
    10741 ggcggcgtag tcggccttct ccgcggggga gagggccctg agccaggcgt cgcggaagtc
    10801 gcgggccagg agggggctgc cgtccgacca cttcgcctcg tcgcggaggt agaaggtgta
    10861 ggtgaggccg tcccgcgagc ggctgaagga gcggcaggcc gccttcacgg gatcgaggtt
    10921 ggaggggtcg taggagaaga gcccctcgta gacgccggtg aagatctgcg cctccgccga
    10981 gtagatggag tgctgggggt cgaactggag ctccagggcg ttgaaggcga cggacagctc
    11041 cgcgggggcg gcggcggggg ccgcgccggc ctcggcggcg gaggccgctc cggcggccag
    11101 ggcgagggcg aggagggcgg cggccattcc atgctgacgg gacattgctg ctccttcgtt
    11161 ccggtcggtg tccggttgca aggtatcgcg cccggcccgc cgtttcaggc cccggggcgc
    11221 gcgcgtcact cggcgcggat gcccaggcgg cgcatctgct cgagctcctc cttctgggcg
    11281 acgtactcgt gcttcttctg gttggccttg atgagggcgc ccttgatcgt cgtgatctcg
    11341 gccttggctc cccggagctt cggacggtcc tcggccggcg tcgccgactt gaagtactcg
    11401 tccagggccg cgatccgctt cacgaggcgc tgcagctcct ccatcgtgcg ctccaggaac
    11461 ttgaaggaca gctcctcggg cgcggtctcg aggcggcgcc atcccggccc gccgcgctgg
    11521 aggaggccgg ccagctgctt ggccttcttg gtgccctcct ccacgaaggc ggcgatgtcg
    11581 atggactcgg tcgtccgggt cccggtcacc gcgtccagga tctccacctc gtaggcgatc
    11641 cccttgtcct gcggggagaa gacgttcctg atggcggcct tgagcctctg gaggaagccc
    11701 gtgttccggg actcgaggac ggaggagttg tccgacatct tctgcacggc gtcctcgagc
    11761 gggtagttga taccggccag gatgcgcacc gcgtccagga cgacgccctt gtaggacttc
    11821 tccttcgcct tctccgtctt cttctcggac ttcacctcca ggcgccggag cagctcgtcc
    11881 cgcagctggg ggccgtcgga ggaatggtcc tccatgagga cctcctccgc gagctccgcg
    11941 tagaagggcc tgtcgccggc gagctcggag aacttgcgcc ggacctgcct cagggcctcg
    12001 tcgcggtggg tgatggcctg ggcggagtcc agggagaggc tcggcatgac gagctgcctg
    12061 agctcgagct tgtagcgctc gcggtggtag gaggcgagct ccttgagggc cgcgaggatg
    12121 tcgcggctcg ccttgtcgag ctgggaggcc gactcggcga tgaggccgga ggagagctgg
    12181 tcggagccgc gcttcaccat gccgacgagc tcggccaggc agcgggtgtt gagctggttc
    12241 gaggtgtcgg agaactgtgt gaagttgaag tacttcacga ggccgaggag gcgcttgatg
    12301 cggcccatcg tgaggaagtc gacggagaac tggtagtagt tgttgaggaa gtcgagatag
    12361 gactcgaact gcgagagcct gaggctcatc tggtcgagct tctcgctctc ggcgaagggc
    12421 ccttcgggcg gggtcgtcac ctccgagatc ttgagctcgt acttgtaggg atcctcgtgg
    12481 acgatgccct tcttgaagag gacgccgtag aggccctgga aggcggtctg gaagagtttc
    12541 caggtctcct tcatcttggg cagttcgtcg cggtcgaggc tgtccctctt cgcgtcgagc
    12601 cgctcctcta ggctcttcgc gaaggcctgg gcttcttcca tagtgaggag agtatggtct
    12661 aaaattccgc cgaaggcaag acttcgcggc cgggagaacc tcccggccgc ggcctggcgg
    12721 ccccgagtgc cggtccgcgg cgtcccgtgg cgcgcccgnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnncc gtcctcaatc gccgacccgg ggagcgcgcg tgggcagggt
    12901 atgcgcgcca tcgtcaggcg gccgcggcgc cgccgcctcg tccccgcctt catccccctc
    12961 ctcgcctgcc tcgcggccgc ctcggcctgc tgcctgccct caggcggcca gcctttcggc
    13021 cccctcgccg cggagcgcct cctcctcgag cttcccgccg cgccggaggc ttggaccggc
    13081 ctccccgagc tctccttcag gctatcctgg cgggacgagg agggccggtt ccgctcggcc
    13141 tggctcgacg cgggctttag gctcgaggcg agggtcaggc gcggcctccc ccaggccctc
    13201 ctcgccgagc cgtacagcct cgggcggccg ctcaggcccg ccggcgccct ctacccggcc
    13261 ggcctcggaa cggagggccg gctgggcctc gactggcgag gtggctgggc ggcgtcggtg
    13321 ttcaggaccc tggaggaggg cggagaggat cccgccgcct acgacttcgg ccgcctgtcg
    13381 cgagagctcg aggcccgggg cggcgatccc tgggcgaccc tcgcgcctct ggcggcggcg
    13441 ggacttctcg cctcgggccg cttccgcgcc gaccggctcg atcccccgac gcgcttcccc
    13501 gtccccctgc ccggccccgg cccttggtgg ccggagagcc ccctcgccgc cgcgccggcg
    13561 cccgattccg aagggggagg ctgggccgtc ggcctcgagc cgggcttcca ccgctactac
    13621 ggctccggcc ggctcctctc cgcgcaggtg gaggctacgg gcgaggccat ggtcctcgtt
    13681 tcgccttagg cgccgccgtt cgtggcgccg ccccccatgg cgccgccccc gctgacggga
    13741 cgctccgggg cacggcgcta ttaagcgccc ttgcccgcgg cggccccgcc cagggggatg
    13801 aggatggtga acagcgagcc ctcgccctcc cagctctcca cggagacgct cccgccgtgg
    13861 gccagggcca cgtgccgcac gatggcgagg cccaggcccg tgccgccctg ctcccgggac
    13921 cgcgccttgt cgacgcggta gaagcgctcg aagacgcggg ggagatcctt cgccgggatg
    13981 ccgatgcccc ggtcccgcac ctctatcctg agccggtccg aggcggggcc ccctgcctcg
    14041 gaccgggccc ggagctccac cgactttccc gagggagagt atttcaccgc gttgtcgagg
    14101 aggttcacga ccgcctgctc gacaaggccg ccgttcagcc cggccttgag gccttcggcg
    14161 cagtccaggc gtatcgcgat gcccttggcc tcggcccggg ggaggacggc ctcgcgggcg
    14221 gcctcgagga ggggcgcgag ctcggtgtcc tcgcggggaa ggccgccctg gccctcctgc
    14281 tcaaggcgcg ccaggacgag gaggtcgtcg atgaggttct ccatcctggc cgcgttgcgc
    14341 tcgatgatcg cgaggaagcg gtccgccttc ccgggatcgg cgagggagcc gccgcggagg
    14401 ttctccgcga agcccttcac gagctggacc ggagtcctga gctcgtggga gacgttggcg
    14461 acgaagtcgc ggcggatcct ctcgagcctt cgcaggcgcg ttatgtcgct caggacgagg
    14521 atgaggccct ccgcggctcc cgcgccgccg gggccggact cttcccggcc gcccggcgcg
    14581 ggggaagcgc ctggcttcgg gaagggggcc gcgacggcct ggaaccagcg ctcgccgccg
    14641 gagtagaggg ctatctcggc ctcgaggcgg ctcccggagg ccagggcctc gcgggcggcg
    14701 gcctcgagct cggtggagcg gctggcctcg aggaggctct tccccaccgc ctcagccgga
    14761 gagccgaggc cgaagagtgc ggccgcggcg ggattcgcga gccgcacgcg gagccggcca
    14821 tccagggaga ggagggcttc cgccatgccg tccaggatgg ccctgcgctc gcggccttcg
    14881 gcctcggcct cggctacgcg agcggcgagc tccgccgcca tcgcctccag gcagcgcccg
    14941 aggacgcgga tctcctctgg gccctccctg acgcggaagg aggaggcgag gggcctgccc
    15001 tcgggagcgc cgagggcggc ggcggccggc tggtcctgcg ccgctacctc ggtcgccgcg
    15061 gcctcatggg acgccgccgc agggcccgcc gagcggccta tcgccgcctc ggccgccgcc
    15121 tccggccgcg ccagcaccgc cgccgccggc cccgccgcgt aggccttcgc ggcctcggcc
    15181 aggcgggcta tggggcggga ggcgcggcgg ctgtagtagg cggccgcggc cagggagacg
    15241 aggcccgcga ggagggcggc cgcggccagg gcgagccgcg ccgggccgag cctcgactcc
    15301 agggcgggaa ggtcggcggc gacgcggagc gctcccgcga cgcggccgtc ccggaggacg
    15361 ggcgccgcgg cgtagaagag ctccatgccg acggtggccg acctgcgccg agccgtgccg
    15421 acgccgcccc gcagggcggc ctccacctcg ggccggcccg agtggttctc catggcggcc
    15481 ggatcggcct tcgaatccgc cgcgacgccg ccgtccaagg cgatgagcgt cacgcggaag
    15541 ctggagcccc cggcggtcct cgccgcgaag cgcgcagccg ccgcgcgcgc cgcggccctt
    15601 tcgccgcccg ccgcgtcggg ggccagggcc ggcagggcct cgtcgggcag ggccgccgcg
    15661 agggagcggg ccgcctcgcc cagggccttc gcgttcgcgt cgccgtagag gccgtccatg
    15721 acgacgaggg cggcgcccgc gacggccacc gtcgcggcga gggcggccgc ggcgatgctc
    15781 ctgaggctct ggccgaagat gcccgccgcc acggtcagtc cccgtcccgg aggcggtagc
    15841 cgacgccgcg caccgtctct atgagctcgc cgcccgggcc gagcttcttc cgtaaggata
    15901 gtatctgaac gtcgaccgag cggtccgtga ccgggtagtc ccggccgcgg acggcgtcga
    15961 tgatctggct gcgggagaag acccagccgg ggctccgcat gaggaagtcc aggatggcga
    16021 actccgtcgc ggagagttcg atgcgcttcc ccccgaactc ggcctggtgg cgggccgagt
    16081 cgaccgtgag gccgccgcgc ttcacccggg tcgcgccggc tgtcgcctgc tccgccagct
    16141 cggcgccccg gcgcagggcc gcgcggatcc gggcggccag gaccttgggg ctgtagggct
    16201 tcacgacgta gtcgtcggcg ccgagctcca ggccggccac gacatccgag tcctcgcccc
    16261 tggccgtcgt catcagcacg gggaggcggc ggcgggaggg ctcggccctg atccgccgca
    16321 agacctcgag gccgtcgatg ccggggagca tgatgtcgag gacgacgcag tccggccttt
    16381 ccttctccag gcgggcaagg ccctcctcgc cgtcgggggc gaggtcgagg atccagccct
    16441 cctgcgcgag cgaggcggcg agcagctccc tgatgtccgg gtcgtcctcg acgatgagga
    16501 ccttggcctt ggccatccct gccccctagt cgttgagctc gacgtggctg ccctcgacca
    16561 tgtagacgac cgcctcgcag acgttcgtca tgtggtcgcc caggcgctcg aggaagcccg
    16621 aggtggtgag gagcctcgcg gcgcgctgga cctggtcggg gcgctccttc atgagctcca
    16681 ggacctcctg gaccagggcc cggtgctcgc cgtcgatgcc gtcgtccagc gcggcgacgc
    16741 gccgcgcctc ctccacgtcg cggttcaggt aagccccgac cgcgccgcgg accatgtcgc
    16801 agccgaccga ggccatcctc tccaggcgct cgatctgcct gaaggggggc tcgcccgaga
    16861 gcttgatcgc ggccttggcg aggtggaccg cgtggtcgcc cgccctctcc aggttgtccg
    16921 tcaccttgag gagggcgacg agctcgcgca ggtcccgggc gacgggcgcc tgggtcgcga
    16981 tgaggacggc cgtctggtcc tcgatcttga gctggagggc gttgacgacc gcgtcgtcgg
    17041 ccttgacctc cttggcgagc tcgacgtcct ggcgcttgag cgcctcgatc gccttgcgga
    17101 ggtcctcctc gacgcgcgtg cccatggaga gcaggtcgtg gtggaggcgg ttcatctcgt
    17161 ccatcagctg tatgcggttt cccatgtcgt tcccccgatg cggatcagcc gaagcggccg
    17221 gagaggtagt tctcggtgcg ctcgtccttc gggttgaaga agagctcctt cgtcggcgcg
    17281 tactcgacga gctcgccgtg gaggaggaag gccgtggagt cggagacgcg gcccgcctgc
    17341 tgcatgttgt gcgtcacgat gattattgta taccgtttct tgagctcgtc gagtaggtcc
    17401 tcgatcttgg aggtggagat cgggtccagg gcgctcgtgg gctcgtccat gaggatgatc
    17461 tcggggcgga cggcgatggc ccgcgcgatg cagaggcgct gctgctggcc gcccgagagg
    17521 gcgaaggcgc tctgcctgag cttgtccttg acctcgtccc agagcgcgac ctggcggagg
    17581 ctcgactcga cgagctcgtc catgtcgccg gagaagccgt tgatcctcgc gccccaggcg
    17641 atgttctcgt ggatggtctt ggggaagggg ttaggcttct ggaagaccat gccgatccag
    17701 cgccgcacct ggacgggatc cacgcccggg tcgtagacgt tgatgccgtg gaagaggacc
    17761 tctccgccgg tcttggtgcc cgggatgagc tcgttcatcc ggttgaaggc ccgcaaggc
//
LOCUS       DAOOSJ010000018        17525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2213_length_17525_cov_6.380137, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000018 DAOOSJ010000000
VERSION     DAOOSJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 17525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17525
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_17525_cov_6.380137"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLG14_01950"
     CDS             <1..594
                     /locus_tag="PLG14_01950"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HOX47921.1"
                     /translation="LRDLEKARTSKTRRWPPAAEFAWVEPAAPGWTADRDGFARAALE
                     AAAANLRAGAFQGLSAGLPELERLPDRLAGLDRAVPAFLLSPALAFMSDSIERNGTGG
                     AAFRTLYAEWLESEAPRLRDPALAAKVSAAAVAARRAEAAWHALAAALSEASRTAKTG
                     RKPEERSRLAASLSDAAKPLVEAERELAAALEAAGGH"
     gene            complement(660..1556)
                     /locus_tag="PLG14_01955"
     CDS             complement(660..1556)
                     /locus_tag="PLG14_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX47922.1"
                     /translation="MLTISLLVDNYVLNEDYAQGEPGLSMHLRLDGGESLLFDFGMTG
                     LFMRNARRMGVDLGGLSWAVLSHGHLDHTWGLGPFMKSLDRVSGGGLGGGAGGGQGAG
                     PGPGGGPASAKPILVCHPKALEAKKYGRFSIGIKARREELEAHFRVEPSAVPRRIAER
                     LVFLGEIERTNEFEGKEPLGRGAEDGRFVDDYMPDDTALAYESDGGLVIVAGCSHSGI
                     CNIVDYARKVTGVERVRAVVGGLHLDASRPELVARTCERLGRQGVESLYACHCTDMKA
                     RIALARTCAVEEAGVGSVLEFA"
     gene            complement(1579..1995)
                     /locus_tag="PLG14_01960"
     CDS             complement(1579..1995)
                     /locus_tag="PLG14_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOX47923.1"
                     /translation="MKSHTVELWFETKRRREFIDITDQVEDALAASGVREGLCLVNAM
                     HITASVFVNDAESGLHQDFLDWLEKLAPHEPVSAYRHNVGEDNADAHLKRQVFGREVV
                     VAVTEGRLHFGPWERIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(1992..2672)
                     /locus_tag="PLG14_01965"
     CDS             complement(1992..2672)
                     /locus_tag="PLG14_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47924.1"
                     /translation="MGSLDLRRANGRAVANELERRGGSRLGVVFSGFGYSYRDPLLRW
                     TKRAFLEKGLDYWGVDLGYRDDPEFMALEPAAQDEAFEADCAVLVRALRELAEGYGGL
                     VLAGKSMGTSVVRRCLKAGLGEGRLALALFTPGTEWRDFAPELAASKVPAFLAASLED
                     PHYVKADLAPIRSRSGLRLFELPRGDHLLETGSLVEDLETQARLFAALLDFLDETGRS
                     VEALRRLR"
     gene            2890..4266
                     /locus_tag="PLG14_01970"
     CDS             2890..4266
                     /locus_tag="PLG14_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX47925.1"
                     /translation="MRRDLTRGSVVGNLVAMSVPTMVGMLGQTLYDVVDLVWVGRISA
                     KAVAGVSLFSSIFWLAEILNEIIGTSSVALITQSFGSGDRGRANRVIEQTIVFKVAVA
                     LVAAALMFVGLEPLVRFFSSDPEVVRAALEYGRIRIFFLPVFFATYSCFTALRCSGDP
                     ASQMWIMLLVSVLNAALDPILMFERVPLLGTRGFGLGVYGAGLATVISITAAFALGFG
                     LLSGGRRNVRLSVRGLFRLDPKIDAKLMTIGLPSGGEMLARQLAGLVTTKFVASYGTA
                     AIAALGIGNRLGGLVFMPLFGLLSGGGTIIGQNLGAENVERAEKTARAASLLGGGAVT
                     LAMGLAIAFPEPVLRVFVDSPETIAVGVPMIRLLGASFIVVSFAIALGSAFSGSGYNL
                     PFLVSSLAGRWGAQVPFLALSAFVLPRAGLELGILGVWASFLVSDLVEAGVLVAAFRK
                     GKWKEHRV"
     gene            complement(4273..5100)
                     /locus_tag="PLG14_01975"
     CDS             complement(4273..5100)
                     /locus_tag="PLG14_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47926.1"
                     /translation="MPNRFIRAVEDGEIGSEAELKSAFRSLALATHPDLAGEGGRSES
                     FVKIRAEYEAALRYLADTGGREGAGRREGSGGEDWSGARGQAGRGGAARGRAFDRGLF
                     YADLAALLKAGFPKTPRHDKERRKYARLRLLVRSSLAARGESPSPLSLFDGFERALSR
                     LKESAEGEPANAGAVGGGPRASAAGTRAAAAETRAAAVLGLLADLVEYEETGIVALRS
                     VIEIELAGLRAEAAAAAADTVAQGGEWARAEEAEAVASLFGFLSFLVADMEGGPALG"
     gene            complement(5116..5952)
                     /gene="nagB"
                     /locus_tag="PLG14_01980"
     CDS             complement(5116..5952)
                     /gene="nagB"
                     /locus_tag="PLG14_01980"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOX47927.1"
                     /translation="MRLIVRPDFEAVSEWAANHVARRINRHERALAERGPGEGGGAPR
                     PFVLGLPTGSSPLGMYRKLAELCAAGKVSFRNVVTFNMDEYVGLPEEHPQSYHSFMWE
                     NFFSRVDIERGNVNILDGNAPDLEAECARYEARMRELGGIDLFVGGVGADGHIAFNEP
                     GSSLSSRTRVKTLTRDTKLMNSRFFGGDPEAVPSTALTVGVGTITDSREVLILVSGLN
                     KARALRHAIEEGVNHLWTVSCLQLHPKGVVVCDEESTMELRVGTVRYFREIESANFDN
                     SI"
     gene            complement(5962..7131)
                     /gene="nagA"
                     /locus_tag="PLG14_01985"
     CDS             complement(5962..7131)
                     /gene="nagA"
                     /locus_tag="PLG14_01985"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOX47928.1"
                     /translation="MPVVCIHNATLLSGYARMENCAVLVEDGLIADVFSERRFEQKRF
                     PADALVLDAGGAHIAPGLIDTHIHGFAGYGTEDLSTDAIMAMSDELGRYGVTAFCPTI
                     YPMAEEDMLASIRAAVAAIGRETGARVMGVHLEGPFISPARLGVQRPEFVRPVDLELM
                     DRFYEAGEGRITNMTVAPEIKGMRELALHCQKKGIVLQAGHTDATYENMLEGMQAGIL
                     HSTHFFNAMSRLHHRDPGAVGAIMIHSELSCEIIADGKHVHPDLIRLLMRDKPSSKVV
                     LVTDSLKPTEQRNCPLFANREEVELSDGLFHRKSDGVIAGSSLTMMRGVSNLASWGVP
                     LENAVEMAASNPARILSLGKRGLVVPGYEADLVVFDKDYRVLASLVGGRFVKNDL"
     gene            7294..8298
                     /locus_tag="PLG14_01990"
     CDS             7294..8298
                     /locus_tag="PLG14_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOX47929.1"
                     /translation="MGKGWIFGIDGGGTSARLRIEEADRGAESRAEGRNDGAGRGRPD
                     RPPLFYGEGGSSNPRSNPREAVLAVLRGLFARAYREAGLRAEGCLGGFAGSAGVDREA
                     DREPFAALVREAAGLPPGVPVLAGNDSEPALAGAIGDVEGLLLIAGTGSIAFGRARDG
                     SSARAGGWGHLLGDEGSAFSLAFKALSRGLRSWEGRDLPTGLMDAGLEFFGLAGPPDL
                     VPLVYEGLDKTRIARFARVVGEYRDRGDPLAFELFEEAERELAALVVSVDARVGPKLE
                     RKRLAFRGGLIEGDARLRAGVESRLAAALPGLEIVPAAADAAAGACILARDLANHGRR
                     "
     gene            8408..>8709
                     /locus_tag="PLG14_01995"
     CDS             8408..>8709
                     /locus_tag="PLG14_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX47930.1"
                     /translation="MDGGGAPARRADLLVQDGLVAAIGDAIKAPEGALVFEAAGAVVS
                     PGFVDAHSHDDAALLEPEGSATKLSQGVTAVVAGNCGLSLAPWTPSEGRPLPPPPL"
     assembly_gap    8710..8809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8810..9826
                     /locus_tag="PLG14_02000"
     CDS             <8810..9826
                     /locus_tag="PLG14_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX47931.1"
                     /translation="VLAGHSSLRAARVADLGGPARPDELKDMIRDLEEGLESGLAGFS
                     GGLAYPASIGAPASEAAALAKAAFSRGKVFALHIRNEYEGMWDALDEAFAIARGALGG
                     SPEPGARLVLSHQKCAGPSNRGRSAELLERLEKAAKDIPLAFDAYPYEAGSTLLEAES
                     VRASRRVLVTWSEPHPELAGLELGEAAERLGLRAEAAIGELSPASAAYFHMDGADVEA
                     ILSHPLCMVGSDGLPNDARPHPRLYGAFPRFFARFVRERRLLTLEEAVRKASSLPASV
                     FGLPGGVLAPGAPADLVVFRPEAIEAGSDWAEPRGTAKGIDLVLVGGAPALGGASLRN
                     PRGR"
     gene            complement(9839..11179)
                     /locus_tag="PLG14_02005"
     CDS             complement(9839..11179)
                     /locus_tag="PLG14_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47932.1"
                     /translation="MHSPRLARLVLASAAALCFLVVPAFASPDARAALPAGYEYASPG
                     CRAALLKAASLADSGAWKSAFAALDEYDPGNADPYALAMKTELCLDGFAQSMMHRLFG
                     LKDLLPGEDLEALRRDGGEFELVGLDPAALSEAQAQAGVAAPPVLSLALGDYFYDVQL
                     RYEGGWFLADEEVRGKAVAAYARAEAGGLLVAQALRRKAELLVVAGDFEGADSGYRAA
                     IALAPGDPELRYRFAVALLMRELRVEALAELDAALAAYAAPESRFEAYVLAARAAAES
                     GDAKRSAAYLDAAEREFPDQPAPGLVRHLLAVEGGAEEAAAAAAEAVMARFPNSPYVV
                     RSLVSSWLGSGKDEAAAAFLDRSIARAGADYEARGGLLFYKALLVAQVRGEAGAAEAL
                     AVLDLAEADFGKAFPPGHEVFGVIAEIRANLLPATGLAPGGASAPDAASAATGE"
     gene            11441..>11998
                     /locus_tag="PLG14_02010"
     CDS             11441..>11998
                     /locus_tag="PLG14_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47933.1"
                     /translation="MPANRKRWVLALCSYIGFLLGLFTAALGPSLGELAKRNGAELGA
                     MGSVFTALFVGALLAQLASGPLSDARGRRAVLVGGLALLAAGASALCLSTALPLALAA
                     VVVAGLGHGSSDLMILVLAAKTYPEKSVSALNILGLLFGAGAFVGPALVGLSQRLSGS
                     ALPVLWFAAAAHALAVPLVLAVLEPE"
     assembly_gap    11999..12098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12099..12855
                     /locus_tag="PLG14_02015"
     CDS             <12099..12855
                     /locus_tag="PLG14_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX47934.1"
                     /translation="AAAHALAVPLVLAVLEPEGKAAAAPSAVPAAKTRDAKPAQAQGD
                     AKAKPVRGALSSPYVWILGLLLLVYVGAENAMGGWMAEYYKLTTGASAPEAAVVVSLF
                     WLALSAGRLAAAAAGTKLSPRAVLGLGIGVALAGALLLALSPGVRVATLAAIVVTGFG
                     YSSIYPTGFAMAAAAFPDAPGKATSLVTVLGSAGGMLLPLLEGFVLAGAGPRAAGPFM
                     LALGLLMLLLFGVAAARPKASRLDREGGAPLAS"
     gene            12869..13561
                     /locus_tag="PLG14_02020"
     CDS             12869..13561
                     /locus_tag="PLG14_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOX47935.1"
                     /translation="MESDDIIPVSLRGSSLERSLHEVESIYAAIDKAEAGFRESAAAA
                     GKPLSCPAGCGSCCEGFVPDVLPLEAAYLAAYILGSRPELAHRPLSGVPPCPFYDADR
                     PEAHCSVYRARPLICRLFAFSAVSGKAEEPSFALCRRMPALPGTEARSWSGEALREAF
                     GAAPPLMSAFAAELLGLDPDSTGERRLLAEALPRALAKVGLALSLAGVAQGDDEPEPN
                     EPEPDAPSPLAA"
     assembly_gap    13795..13894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13895..14925
                     /locus_tag="PLG14_02025"
     CDS             <13895..14925
                     /locus_tag="PLG14_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47936.1"
                     /translation="PDSPRPGAPRSAAGRGAKALSVSRLDLEAEGAAAALPPGPFDAV
                     LMGRAVNELWKGREDRLERRLALLEAAQARLAPGGFLLVLEPAVLLSSREALELRDRL
                     AARGAPILGPCLRQGPCPALAAGPGHSCHGEYPWSPPEPVASLARRAGLDRSSVKTTW
                     FAAGRPGDTSPGAARAEAFQPAPSPAPAEALPAGARKAAPSVGAPREAASPREGAGPG
                     GVIRALVVSEPMLNKAGRTRYLLCGEEGRRAFSAAKDDGRAQAAGFRTLGRYDIVRIT
                     RPEARPGQGEGKGRGAAAAGRAGSPAARSAAGLGQAEPREEAARGEAAAIGFGPETLL
                     EIERAPRP"
     gene            14935..>15347
                     /gene="arfB"
                     /locus_tag="PLG14_02030"
     CDS             14935..>15347
                     /gene="arfB"
                     /locus_tag="PLG14_02030"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOX47937.1"
                     /translation="MDKERLRASIEAACEMDFARSGGPGGQNVNKVNSKVVARVRMDR
                     LEGLSEAEAARARELLSSRLTNEDELLVVADEERDQLRNREAALERLFALVSAAAAIP
                     KKRRPTKPTRASRERRLSAKRGRSEDKRLRGKPPA"
     assembly_gap    15348..15447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15490..16176
                     /locus_tag="PLG14_02035"
     CDS             15490..16176
                     /locus_tag="PLG14_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOX47938.1"
                     /translation="MGRKLIVSAVGWSGSGKTSFLERAVAECARRGIPCSAAKATRHA
                     GDFEPEGKDSARYRAAGAGEVAFVGLARGPEGPAGLTVLFEPTPSEPDRAFLERLFPG
                     SPLVFAEGLVVEGALRLLVAGPAPGEEGLKRPLDWADILVTADPALAALAKGLGKAVV
                     RPDAAATFVDMLEELMERDVTVTSGGKAVPLNPFVKEIVANTVAALVGCLKKADADEE
                     IVVTIKPASK"
     gene            16353..17288
                     /locus_tag="PLG14_02040"
     CDS             16353..17288
                     /locus_tag="PLG14_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47939.1"
                     /translation="MRKYPSRRGDKPLELCALLDHGTPRLVRAEAFRLFRAWHPSSAF
                     LPVKRVFAAVTRLYSGRFPGFKACSTEYHDLYHSLEVFSSSARLLDGALLAGRRIGAE
                     GAVDLLAAALLHDAGYIQEEGDGSGTGAKFTKTHVDRSAAFTRSHARELGLDDRRAAR
                     VSRIILGTDLARQWSGLAFESEEEGFLASILAAADLLGQMADRAYLERLLFLYYEFRE
                     AGIEGYATAFDILKKTAGFYSAVKARLDGPLGGASAYARLHFARRYGEDRDLYREAID
                     RQMAYLDAIVADASTNFRKKLHRLDLEAVERERRA"
BASE COUNT         2265 a         6447 c         6026 g         2387 t
ORIGIN      
        1 ctccgggacc tcgagaaggc ccgcacttcg aagaccaggc gatggcctcc cgcggcggag
       61 ttcgcctggg tggagccggc tgccccgggc tggacggcgg accgcgacgg cttcgcgcgc
      121 gcggcgcttg aagccgccgc cgccaacctc cgcgccggcg ccttccaggg cctgtccgcc
      181 ggcctgcccg agctggagcg cctgccggac aggctcgccg ggctggaccg ggctgtcccg
      241 gctttcctgc tgtcgccagc cctcgcgttc atgtcggact cgatcgagcg gaacggcacg
      301 ggcggcgcgg ccttcaggac cctctacgcc gagtggctcg agtcggaggc gccgaggctc
      361 cgcgatccgg ccctcgccgc gaaggtctcc gccgccgccg tcgccgcccg ccgggccgag
      421 gcggcctggc atgccctcgc ggccgccctg tcggaggcct ctcggacggc caagacgggg
      481 agaaagcccg aagagcggtc caggctggcc gcgagcctat cagacgcggc caagcccctc
      541 gtcgaggccg aacgggagct ggccgccgcc ctggaggcgg cgggcggaca ctgaggccgg
      601 ctgacttcga ggccggcggt caagttccct ggctccggcg gcccgcttcc tcggcggcct
      661 cacgcgaact cgaggaccga gcccacgccc gcctcctcga ccgcgcaggt ccgggccagg
      721 gcgatgcggg ccttcatgtc ggtacagtgg caggcgtaga ggctctccac gccctggcgc
      781 cctaggcgct cgcaggtcct cgcgacgagc tccggccgcg aggcgtcgag gtggaggccg
      841 ccgaccaccg ccctgacgcg ctcgacgccc gtcaccttcc tcgcgtagtc gacgatgttg
      901 cagatccccg aatgcgagca gcctgcgacg atgacgaggc cgccgtccga ttcgtaggcg
      961 agggccgtgt cgtcgggcat atagtcgtcg acgaagcggc cgtcctccgc ccccctgcca
     1021 agcggctcct tgccctcgaa ctcgttcgtc cgctcgatct cgcccaggaa gacgaggcgc
     1081 tcggctatcc ttcgcgggac ggcggaaggc tccacacgga aatgggcctc gagctcctcg
     1141 cgccgggcct tgatcccgat cgagaagcgg ccgtacttct tggcctcgag agccttcgga
     1201 tggcagacca ggatcggctt ggcgctggcg gggccgccgc caggcccagg gccggcccct
     1261 tgcccgccgc ccgcccctcc gcccaggcct ccgccggaga cgcgatccag gcttttcatg
     1321 aagggcccca ggccccaggt gtggtcgagg tgcccgtggg agagcacggc ccacgagagg
     1381 ccgccgaggt cgacgcccat gcgccgcgcg ttcctcatga agaggccggt catgccgaag
     1441 tcgaagagta ggctctcccc gccgtccagg cgaaggtgca tggagaggcc gggctccccc
     1501 tgcgcgtagt cctcattgag cacgtagttg tccacgagca ggctgatggt cagcatcgct
     1561 ccctccgcgg ccgcgccgct actccccgat gatcttcacg aggacccgtt tccgcctccg
     1621 accgtcgaac tcgccgtaga agatccgctc ccagggcccg aagtggagcc ggccctccgt
     1681 gaccgcgacg acgacctccc gcccgaagac ctggcgcttc aggtgcgcgt ccgcgttgtc
     1741 ctcgccgacg ttgtggcggt aggccgaaac aggctcgtgg ggggcgagct tctcgagcca
     1801 gtccaggaag tcctggtgca gcccggactc cgcgtcattg acgaagacgg aggcggtgat
     1861 gtgcatggcg ttcacgaggc aaagcccttc cctgacgccc gacgcggcga gcgcgtcctc
     1921 cacctggtcg gtgatgtcga tgaattcgcg gcgcctcttc gtctcgaacc agagctccac
     1981 ggtgtgggac ttcatcgcag cctcctcagc gcctcgacgc tcctgccagt ctcgtcgagg
     2041 aagtcgagga gggcggcgaa gaggcgcgcc tgggtctcga ggtcctcgac caggctcccc
     2101 gtctcgagga ggtggtcgcc ccgagggagc tcgaaaaggc ggaggcccga cctagaccgg
     2161 atcggcgcga gatccgcctt gacgtaatgc ggatcctcga ggctcgcggc gaggaaggcg
     2221 gggactttcg aggcggcgag ctcgggcgcg aagtcccgcc actcggtgcc gggagtaaag
     2281 agggcgaggg cgagccgacc ctcccccagc cccgccttga ggcagcggcg gacgacgctc
     2341 gtgcccatgg acttccccgc gaggacgagc ccgccgtagc cctcggccaa ctcgcggagg
     2401 gcgcggacga ggacggcgca gtccgcctcg aaggcctcgt cctgggcggc cggctccagg
     2461 gccatgaact ccggatcatc gcggtagccg aggtcgacgc cccagtagtc gaggcccttc
     2521 tccaggaagg cgcgcttggt ccagcggaga agggggtcgc ggtagctgta gccgaagccg
     2581 gagaacacga cgccgagccg gcttccgccg cgccgctcga gctcgttcgc gacggcgcgg
     2641 ccgttcgcgc gcctcaggtc gagggatccc atgctgcctc ctcgggcccg ggagcccggc
     2701 gcgcgcccgg agccgctttc acagtgaaag cataagcccg ccccgcccgg ctcgccaccc
     2761 tcactacggg gcagccgccg tccgcggatc cacccgcggc gtcggcgccg cgccggggac
     2821 gggagcggcc gcgagggcgg accgcgccgc tccttgaccc cgcgctgccc cgcgcactat
     2881 acatacgtta tgcgccgcga tttgaccagg ggaagcgtcg tggggaacct cgtcgcgatg
     2941 tccgtgccga cgatggtcgg gatgctcgga cagacgctct acgacgtggt ggacctcgta
     3001 tgggtcggcc ggatctcggc caaggcggtg gcgggcgtct ccctcttcag ctccatcttc
     3061 tggctggccg agatcctcaa cgagatcatc ggcacctcct ccgtcgccct catcacgcag
     3121 agcttcggct cgggcgaccg cgggcgcgcg aaccgcgtca tcgagcagac catcgtcttc
     3181 aaggtcgcgg tggccctcgt cgcggccgcc ctcatgttcg tcggcctcga gcccctggtg
     3241 cgcttcttct cgagcgaccc cgaggtggtc cgcgccgcgc tcgaatacgg gcggatccgg
     3301 atcttcttcc tccccgtctt cttcgcgacc tactcctgct tcaccgccct ccgctgctcg
     3361 ggcgaccccg cgagccagat gtggatcatg ctcctcgtct ccgtcctcaa cgcggccctc
     3421 gacccgatcc tcatgttcga gagggtgccc ctcctcggga cgcggggctt cgggctcggc
     3481 gtctacggcg cgggcctcgc cacggtgatc tccatcacgg ccgccttcgc cctcggcttc
     3541 ggcctcctct cgggcggacg ccggaacgtc aggctctcgg tccgcgggct cttccgcctc
     3601 gaccccaaga tcgacgcgaa gctcatgacg atcggcctcc cctccggcgg cgagatgctc
     3661 gcgcgccagc tcgcgggcct cgtcacgacg aagttcgtcg cctcctacgg cacggccgcg
     3721 atcgcggccc tgggcatcgg gaaccgcctc ggaggcctcg tcttcatgcc cctcttcggc
     3781 ctcctgtcgg gcggcggcac gatcatagga cagaacctcg gagcggagaa cgtcgagcgc
     3841 gcggagaaga ccgccagggc cgcctccctc ctgggcggcg gggcggtgac cctcgccatg
     3901 ggcctcgcca tcgccttccc ggagccggtc ctgcgcgtct tcgtcgactc gccggagacg
     3961 atcgccgtcg gcgtacccat gatcaggctc ctcggggcct ccttcatcgt cgtgagcttc
     4021 gcgatcgccc tcggctcggc cttctcgggc tcgggctaca acctgccctt cctcgtctcc
     4081 tcgctcgccg ggcgctgggg cgcccaggtc cccttcctcg cgttgtccgc cttcgtcctc
     4141 ccccgcgcgg gactggagct cggcatcctg ggcgtctggg cgagcttcct cgtctccgac
     4201 ctcgtggagg ccggcgtcct cgtcgcggcc ttcaggaagg gcaagtggaa ggagcaccgg
     4261 gtctaggcgg ccctagccca gggccggccc gccctccatg tccgcgacca ggaaggagag
     4321 gaagccgaag agcgaggcga cggcctcggc ctcctcggcg cgggcccact cgccgccttg
     4381 ggcgacggta tcagcggccg cggccgccgc ctcggcgcgg aggccggcga gctcgatctc
     4441 tatcacggag cgcagggcga cgatgcccgt ctcctcgtac tcgacgaggt cggcgaggag
     4501 gcccaggacc gcggccgcgc gcgtctccgc cgcggccgcg cgggttccgg cagcggacgc
     4561 gcgcgggccg ccgccgaccg cgccggcatt ggccggctct ccctcggcgc tctccttgag
     4621 ccgcgacagg gcgcgctcga agccgtcgaa gagcgagagg ggcgaggggg actcgccgcg
     4681 cgcggcgagg gaggagcgga cgaggagcct gagcctcgcg tacttgcgcc tctccttgtc
     4741 atggcggggc gtcttgggga agcccgcctt gagcaaggcc gcgagatcgg cgtagaagag
     4801 gccccggtcg aaggccctcc cccgcgcggc tcctccccgg cccgcctggc cgcgcgcccc
     4861 cgaccagtcc tccccgccgc tcccctcgcg ccttcccgcc ccttcgcgcc cgcccgtgtc
     4921 ggcgaggtag cggagggccg cctcgtactc ggcgcggatc ttcacgaagg actcgctcct
     4981 cccgccctcg ccggcgaggt cgggatgggt cgcgagggcc agggacctga aggcggactt
     5041 aagctccgcc tcggagccta tctccccgtc ctcgacggcg cggatgaagc ggttcggcat
     5101 cctggaggcc ggcccctaga tcgaattgtc gaagttggcc gactcgatct cccggaagta
     5161 gcggaccgtg ccgaccctga gctccatggt cgactcctcg tcgcagacga cgacgccctt
     5221 cgggtggagc tggaggcagc tcacggtcca gaggtggttc acgccctcct cgatcgcgtg
     5281 gcgcagggcc cgcgccttgt tgaggcccga gacgaggatg aggacctccc gggagtcggt
     5341 gatggtgccg acgccgacgg tgagggcggt ggagggcacg gcctccgggt cgccgccgaa
     5401 gaagcgcgag ttcatgagct tcgtgtcgcg ggttagggtc ttcacgcgcg tgcgcgagga
     5461 gagggaagag cccggctcgt tgaaggcgat gtgcccgtcc gcgcctacgc ccccgacgaa
     5521 gaggtcgatg ccgccgagct ccctcatcct ggcctcgtag cgcgcgcact cggcctcgag
     5581 gtcgggcgcg ttgccgtcca ggatgttgac gttgccgcgc tcgatgtcca cgcgcgagaa
     5641 gaaattctcc cacatgaagc tgtggtagct ctgcgggtgc tcctccggga ggcccacgta
     5701 ctcgtccatg ttgaaggtga cgacgttgcg gaaggagacc ttgcccgcgg cgcagagctc
     5761 ggcgagcttc cggtacatgc cgaggggcga agagcccgtc ggaaggccca ggacgaaggg
     5821 ccgcggcgcc cctccgccct cccccggccc ccgctcggcc agggcgcgct cgtgcctgtt
     5881 gatccggcgg gccacgtggt tcgccgccca ctcggagacg gcctcgaaat cgggcctcac
     5941 tatgagtcgc atatcctctc cttagaggtc gttcttgacg aagcggccgc cgaccaggct
     6001 cgccaggacg cggtagtcct tgtcgaacac gactaggtcg gcctcgtagc ccgggaccac
     6061 gaggcctcgc ttcccgaggc tcaggatgcg cgccgggttc gaggcggcca tctccaccgc
     6121 gttctccagg gggacgcccc aggaagccag gttggagacg ccgcgcatca tcgtgagcga
     6181 gctgcccgcg atgacgccgt cggacttgcg gtggaagagg ccgtcggaga gctcgacctc
     6241 ctcgcggttc gcgaagaggg ggcagttacg ctgctcggtg ggcttgaggg agtcggtgac
     6301 caggacgacc ttgctcgagg gcttgtcgcg catgaggagc ctgatgaggt cggggtggac
     6361 gtgcttgccg tccgcgatga tctcgcagga cagctccgag tggatcatga tggcgcccac
     6421 cgcgccgggg tcgcggtggt ggagccgcga catggcgttg aagaagtgcg tggagtggag
     6481 gatgcccgcc tgcatgccct ccagcatgtt ctcgtaggtg gcgtcggtgt ggccggcctg
     6541 gagcacgatg cccttcttct ggcagtggag ggcgagctcc ctcatgccct tgatctccgg
     6601 cgcgaccgtc atgttggtga tgcggccctc tcccgcctcg tagaagcggt ccatgagctc
     6661 gaggtcgacg ggccgcacga actcggggcg ctggacgccg agccgggcgg gggagatgaa
     6721 gggcccctcc aggtggacgc ccatgacgcg ggctcccgtc tcgcggccga tcgcggcgac
     6781 ggccgcgcgg atggaggcga gcatgtcctc ctcggccatc gggtagatgg tcggacagaa
     6841 ggcggtgacg ccgtagcgcc cgagctcgtc ggacatcgcc atgatggcgt cggtggagag
     6901 gtcctccgtg ccgtagccgg cgaagccgtg gatgtgggtg tctatgagac cgggcgcgat
     6961 gtgcgcgccg cccgcgtcca ggacgagggc gtcggccggg aagcgcttct gctcgaagcg
     7021 ccgctcggag aagacgtcgg cgatgaggcc gtcctccacg aggacggcgc agttctccat
     7081 cctcgcgtag cccgagagca gcgtggcgtt gtggatgcag acgaccggca aggcgcacct
     7141 ccccgctccg ggcctggcgt tggccggggg cggcttcctg tcttcaacaa tacgacgcgc
     7201 gatggcctcg aggcaaggcg ggccgcctct ttgcccgggc gcttcctccc cgccagccgc
     7261 ccgggctaga atggccgtcg aaagggaaag ctcatgggca aaggctggat cttcggcatc
     7321 gacggcggcg ggacgagcgc ccgcctgaga atagaagagg cggaccgcgg cgcggaaagc
     7381 cgggcggaag gccggaatga cggagcaggg agaggccggc ccgaccggcc gcccctcttc
     7441 tacggcgaag gcgggagctc gaatccgcgc tccaatcccc gcgaggcggt cctcgcggtc
     7501 ctgcgggggc tcttcgcccg cgcctatcgc gaggcgggcc tccgggccga aggctgcctg
     7561 ggcggcttcg cgggctcagc cggcgtggac cgcgaggccg accgcgagcc cttcgcggcc
     7621 ttggtccggg aagccgcggg cctcccccct ggcgtccccg tcctggcggg caacgactcc
     7681 gagccggccc tcgcgggggc gataggcgac gtcgagggcc tcctcctcat cgcgggcacg
     7741 ggctccatcg ccttcgggag ggcgcgggac ggcagctcgg cgagggccgg aggctggggc
     7801 caccttctcg gcgacgaggg atccgccttc agcctcgcct tcaaggccct ctcgcgcgga
     7861 ctccgctcct gggagggacg ggacctgccg acgggcctca tggacgcggg gctcgagttc
     7921 ttcggcctgg ccggcccgcc cgacctcgtg cccctcgtct acgagggcct cgacaagacg
     7981 aggatcgcgc gcttcgcccg cgtcgtcggc gagtaccggg acaggggcga ccccctggcc
     8041 ttcgaactct tcgaggaggc ggagcgggag ctcgccgccc tggtcgtctc cgtggacgcc
     8101 cgcgtcgggc ccaagctcga gaggaagagg ctcgccttcc gcggcggact catcgaggga
     8161 gacgcgaggc tcagggccgg ggtcgagtcg cggctcgcgg cggccctgcc cggcctggag
     8221 atcgtccccg ccgccgccga cgccgcggcc ggcgcctgca tcctggcccg agacctggcg
     8281 aaccatggcc ggcggtgacg cccgggaggg ccgcccgggc cgcgaggccc gggcgccgtc
     8341 gaaaacgccg tcgggcgcgg ccggcgcccg acgcggagcg tccgtcctcg tccgcggggg
     8401 gctcctcgtg gacggcggcg gagccccggc tcggcgcgcc gacctcctcg tccaggacgg
     8461 cctcgtcgcc gcgataggcg acgccatcaa ggctcccgaa ggcgccctcg tcttcgaggc
     8521 cgcgggggcc gtcgtctccc cgggcttcgt ggacgcccat tcccacgacg acgcggccct
     8581 cctcgagccc gagggctccg cgaccaagct ctcgcagggc gtcaccgccg tcgtcgcggg
     8641 caactgcggc ctgagcttgg cgccctggac gccctcggag ggccgtcccc ttccgccgcc
     8701 tcccctcaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcctcgcggg
     8821 ccactcctcg ctccgcgccg cgcgggtggc cgacctcggc ggcccggcgc ggccggacga
     8881 gctcaaggac atgatccggg acctggaaga ggggctcgag tcgggcctcg cgggcttctc
     8941 gggcggcctc gcctatcccg cctcgatcgg agcgcccgcc tcggaggctg cggccctggc
     9001 caaggccgcc ttctcccgcg gcaaggtctt cgccctgcac atccgcaacg agtacgaagg
     9061 catgtgggac gccctggacg aggccttcgc gatcgcgcgc ggggccctgg gcggctcgcc
     9121 tgagcccggc gcgcggctcg tcctctccca ccagaagtgc gcggggccct cgaaccgggg
     9181 ccgctccgcc gagctcctcg agcgcctgga gaaggcggcg aaggacattc ccctcgcctt
     9241 cgacgcctat ccctacgaag ccggctcgac cctcctggag gccgagtcgg tccgggcctc
     9301 gcgccgcgtc ctcgtgacct ggtcggagcc ccaccccgag ctcgccggcc tggagctggg
     9361 cgaggccgcc gagaggctcg gcctacgggc ggaggccgcg ataggcgaac tatccccggc
     9421 cagcgccgcg tatttccaca tggacggggc cgacgtcgag gccatcctct cccacccgct
     9481 gtgcatggtc ggctccgacg gcctgcctaa cgacgcccgg ccccatcccc ggctctacgg
     9541 cgccttcccc cgcttcttcg cccgcttcgt ccgcgagcgc cggctcctca cgctggagga
     9601 agccgtccgc aaggcgagct ccctgccggc ctcggtcttc ggccttcccg gcggcgtcct
     9661 cgcgccggga gcgcccgccg acctcgtcgt cttccgcccc gaggccatcg aggcgggctc
     9721 ggactgggct gagccgcgcg ggacggccaa gggcatcgac ctggtcctcg tcggaggcgc
     9781 cccggccctc ggcggcgcga gcctccggaa cccgcgcggc cgctgagccg gcccggggct
     9841 actcgccggt cgcggccgaa gccgcgtcgg gggcggacgc tccgcccggc gccagcccgg
     9901 tggccgggag gagattcgcg cggatctccg cgatgacgcc gaagacctcg tgcccgggcg
     9961 ggaaggcctt gccgaagtcc gcctcggcga ggtcgaggac ggcgagggcc tcggccgcgc
    10021 ccgcctcgcc cctcacctgg gcgacgagga gggccttgta gaacaggagg ccgccccggg
    10081 cttcgtagtc cgcgccggcg cgggcgatgg agcggtccag gaaggcggcg gccgcttcgt
    10141 ccttccccga gccgagccag ctcgagacga gggagcggac cacgtagggg gaattcggga
    10201 agcgggccat gacggcctcc gcggccgcgg cggcggcctc ctcggcccct ccctcgacgg
    10261 ccaggaggtg gcggacgagg cccggcgcgg gctggtcggg gaactcccgc tccgccgcgt
    10321 ccaggtaggc ggccgaacgc ttcgcgtcgc ccgactcggc agcggcccgc gcggccagga
    10381 cgtaggcctc gaagcggctc tccggcgcgg cgtaggcggc gagggcggcg tccagctcgg
    10441 cgagggcctc gacgcggagc tcgcgcatga ggagggcgac cgcgaagcgg tagcggagtt
    10501 cggggtcgcc tggagcgagg gctatcgcgg cgcggtatcc cgagtcggcg ccctcgaagt
    10561 ctcccgcgac gacgaggagc tcggccttcc tgcggagggc ctgggcgacg aggaggccgc
    10621 cggcctccgc cctggcgtag gcggcgaccg ccttcccccg cacctcctcg tcggcgagga
    10681 accagccgcc ctcgtagcgg agctggacgt cgtagaagta gtcgcccagg gccagggaga
    10741 ggacgggggg cgccgcgacg ccggcctggg cctgggcctc cgagagggcc gcgggatcca
    10801 ggcccacgag ctcgaactcc ccgccgtccc tccgcagggc ctcgaggtcc tcgccgggga
    10861 ggaggtcctt gagcccgaag aggcgatgca tcatgctctg cgcgaagccg tccaggcaga
    10921 gctcggtctt catggcgagg gcgtaggggt cggcgttccc agggtcgtac tcgtccaggg
    10981 cggcgaaggc gcttttccag gcgcccgagt ccgcgaggga ggcggccttg agaagggcgg
    11041 cgcggcagcc cggagaggcg tactcgtagc cggcgggcag ggctgccctc gcgtcaggcg
    11101 acgcgaaggc ggggacgacg aggaagcaaa gcgcggcggc cgaagccagg acgaggcgcg
    11161 caaggcgcgg actgtgcatg ggaactcctc tcggggatgg gcggaagccg ggaaacggta
    11221 cagccggccg ccttccgcgg tcaagcctcg gagcctccac cggcccgtcg ccggacgcgt
    11281 cgacggaacc gtcgccgtga ccgtcgtatt gacgagcctc cagcctcggg cctatcatcc
    11341 cgcgaccgcg ccttcgcggc ggccgcgcgt ccgccgcgcg gcgacgccag ccgctcccgg
    11401 cgccggcgcg gcacagaacc cagcccgaca aaggaactcc atgcccgcaa accggaagag
    11461 atgggtgctc gccctctgca gctacatagg cttcctcctc ggcctcttca cggcggccct
    11521 cggcccgagc ctaggcgagc tcgcgaagcg gaacggcgcc gagctcggcg cgatgggctc
    11581 ggtcttcacc gccctcttcg tgggggccct cctggcccag ctcgcctccg gccccctgag
    11641 cgacgcgcgg gggcggcggg ccgtcctcgt cggcggcctc gccctcctcg cggcgggagc
    11701 ctcggccctc tgcctctcca cggccttgcc cctcgccctc gcggccgtcg tcgtcgcagg
    11761 gctcgggcac ggctcctccg acctcatgat cctcgtcctc gcggccaaga cctatcccga
    11821 gaagagcgtc tccgccctca acatcctggg cctcctcttc ggcgccggcg ccttcgtcgg
    11881 cccggccctc gtgggcctct cgcagaggct ctcgggcagc gccctccccg tcctctggtt
    11941 cgcggccgcc gcccacgccc tcgccgtccc cctcgtcctg gcagtcctgg agcccgagnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ccgccgccca cgccctcgcc
    12121 gtccccctcg tcctggcagt cctggagccc gagggcaagg ccgccgcggc tccgtcggcg
    12181 gttcccgcgg caaagacccg ggacgcgaag cccgcccagg cgcaggggga tgccaaggcg
    12241 aagcccgtcc gaggcgccct ctcctcgccc tacgtctgga tcctcggcct cctcctcctc
    12301 gtctacgtcg gcgcggagaa cgcgatgggc ggctggatgg ccgagtacta caagctcacg
    12361 accggcgcga gcgcccccga ggccgccgtc gtggtctccc tcttctggct cgccctctcc
    12421 gcgggcaggc tggccgccgc cgcggccggc acgaagctct ccccccgggc cgtccttggc
    12481 ctcggcatcg gcgtagcctt ggccggagcc ctcctcctcg cgctgtcgcc cggagtccgc
    12541 gtagcgaccc tcgccgccat cgtggtcacg ggcttcggct acagctccat ctacccgaca
    12601 ggcttcgcga tggcggccgc cgccttcccc gacgcgccgg gcaaggcgac gagcctggtc
    12661 accgtcctcg ggagcgccgg cggcatgctc ctccccctcc tcgagggctt cgtcctcgcc
    12721 ggcgcggggc ctcgggccgc ggggcccttc atgctggccc tcggcctact catgctccta
    12781 ctcttcggcg tcgcggccgc gcggcccaag gcctcccggc ttgacaggga gggaggcgcg
    12841 ccgctagctt cctagccgga aaatccacat ggaaagcgac gacatcatcc cggtttccct
    12901 gcgaggctcc tccctggaga ggagcctcca tgaagtcgaa tcgatctacg cggccataga
    12961 caaggccgag gccggcttcc gcgagtcggc cgccgcggcc ggcaagcccc tctcctgccc
    13021 cgccggctgc ggctcctgct gcgagggctt cgtccccgac gtcctccccc tggaggcggc
    13081 ctacctcgcg gcctacatcc tgggctcgag gccggaactc gcccacaggc ccctctcggg
    13141 cgtccccccc tgccccttct acgacgcgga ccggcccgag gcgcactgct cggtctaccg
    13201 cgcgaggccc ctcatctgca ggctcttcgc cttctccgcc gtctcgggca aggccgagga
    13261 gccgagcttc gccctctgcc gccggatgcc cgccctcccg ggcaccgagg ccaggtcctg
    13321 gtccggcgag gccctccgcg aggccttcgg cgccgcgccg cccctcatgt cggccttcgc
    13381 cgccgagctc ctcggcctgg accccgactc gacgggcgag cggcgcctcc tggccgaggc
    13441 cctgccccgc gctctcgcga aagtgggcct ggccctgagc ctcgccggcg tggcccaggg
    13501 cgacgacgag cccgagccga acgagcccga gcccgacgcg ccttccccgc tcgcggcctg
    13561 agcccgaaga accttcactg gcgcgtcccg agcctctggc ccgtcggacc cgctcgcttg
    13621 cccgccgcat ccctttcgtc catactcggg aagcggttcc gacaaggaac ttccaaggag
    13681 cgcaggtgaa caaggctttc cgccccggcg agaggaagga gaggcgcccg gcgggccctc
    13741 tcgggctggc cctggaggcc cgccttcccg ccctcgccga gctctggcgc gccgnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcccg acagtccgcg gccaggcgcc
    13921 ccccggagcg ccgcgggccg cggagcgaag gcgctgtccg tctcgcgcct cgacctcgag
    13981 gccgaaggcg cggccgccgc cctgcctccc ggccccttcg acgccgtcct catgggcagg
    14041 gcggtcaacg agctctggaa gggcagggag gaccgactgg agaggcggct cgccctcctc
    14101 gaggccgccc aggcccggct cgcgcccgga ggcttcctcc tcgtcctcga gccggccgtc
    14161 ctcctctcga gccgcgaggc cctcgagctc agggaccgcc tggccgcaag gggcgcgccc
    14221 atcctcggcc cctgcctcag gcaggggccc tgccccgccc tggcggccgg ccctggccac
    14281 agctgccacg gggagtatcc ctggagcccg ccagagcccg tcgcctccct cgcgcgccgc
    14341 gccggcctgg accggagctc agtgaagacg acctggttcg ccgcgggccg gccgggggat
    14401 acgtctcctg gcgcggctcg cgcggaggcg ttccagccgg ctccctcccc cgcgcccgcc
    14461 gaggcgcttc ccgccggcgc gcgcaaggcg gcgccttccg tgggcgcgcc ccgggaagcc
    14521 gcctcgccgc gggaaggcgc cggtcccggc ggggtcatcc gggccctcgt cgtctccgag
    14581 cccatgctca acaaggcggg aaggacccgc tacctcctct gcggcgagga ggggcgcagg
    14641 gccttctccg cggcgaagga cgacggccgc gcccaggccg ccggcttccg gaccctcgga
    14701 cgctacgata tcgtccgcat aacgcgcccc gaggcgaggc ccggccaggg cgaagggaag
    14761 ggccggggcg cggcggccgc gggacgcgcc ggaagcccgg ccgcccgcag cgccgccggc
    14821 ctgggccaag ccgagccccg ggaagaggcc gcccgcggcg aggccgcggc catcggcttc
    14881 ggccccgaga ccctgctcga gatcgagcgg gcgccgcgcc cgtaggaggg gctgatggac
    14941 aaggaaaggc tcagggcctc catagaggcg gcctgcgaga tggatttcgc ccgctccggc
    15001 ggcccgggag gccagaacgt caacaaggtc aactccaagg tcgtcgcgag ggtcaggatg
    15061 gataggctcg agggcctctc ggaggccgag gccgcgaggg ccagggagct cctctcgagc
    15121 cgcctcacga acgaggacga gctcctcgtc gtggccgacg aggagcggga ccagctccga
    15181 aaccgcgagg cggccctgga gcgcctcttc gccctggtct cggcggccgc ggccatcccc
    15241 aagaagcggc gtccgaccaa gccgacccgg gcctcgcggg agcgccgcct ctccgccaag
    15301 cgcggccgct cggaggacaa gcgcctccgc ggcaagccgc cggcggannn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnncct cgattgcgcg gcaagggcgg ctccggctat
    15481 agtcctgcca tgggcaggaa gctgatcgtc tccgcggtgg gctggtcggg aagcggcaag
    15541 acgagcttcc tggagagggc agtcgccgag tgcgccaggc ggggcatccc ctgctcggcg
    15601 gccaaggcca cccgccacgc gggcgacttc gagcccgagg gcaaggactc ggcccgctac
    15661 cgggcggccg gagcggggga ggtcgccttc gtcggcctcg cacgcgggcc cgagggcccg
    15721 gccggcctca ccgtcctctt cgagccgact ccttccgagc ccgaccgggc cttcctggaa
    15781 aggctcttcc ccggctcgcc cctcgtcttc gccgagggcc tggtcgtcga gggcgccctc
    15841 aggctcctcg tcgccggccc ggccccgggc gaggagggcc tcaagcgccc cctcgactgg
    15901 gccgacatcc tcgtcaccgc cgatccggcc ctcgcggctc tcgcgaaggg cctcgggaag
    15961 gccgtcgtcc gcccggacgc ggccgccaca ttcgtcgaca tgctggagga actcatggaa
    16021 cgcgacgtga ccgtcacctc ggggggcaag gccgtcccgc tgaacccctt cgtcaaggag
    16081 atcgtggcca acacggtcgc cgccctcgtg ggctgcctca agaaggccga cgccgacgag
    16141 gagatcgtcg tcacgatcaa gccggcctcc aagtagggcg aaggggcggc tctcccggag
    16201 ccgccacacc tgggaaaaac cctatcgggg ccgggcgggc ttctcccttt cggggcaagg
    16261 acgtggccaa ggccgttcca cgcgctatac ttaagggatg gaagcccggc caggcccctg
    16321 gggcaaggcc gcgcctcggg aaagaggctc gcatgcgaaa atatccaagc cggaggggcg
    16381 acaagcccct ggagctttgc gccctgctcg accacggaac acccaggctg gtgcgcgccg
    16441 aggccttccg cctcttccgc gcctggcatc cctcctcggc cttcctgccc gtgaagcgcg
    16501 tcttcgcggc cgtcacgagg ctctactccg gccgcttccc cggcttcaag gcctgcagca
    16561 ccgaatacca cgacctctac cacagcctgg aggtcttctc ctcctcggcc cgcctcctgg
    16621 acggagccct cctcgccggc cgcaggatcg gggcggaagg cgcggtcgac ctcctggccg
    16681 ccgcactcct ccatgacgcg ggctacatcc aggaggaggg cgacggatcg ggaaccggag
    16741 ccaagttcac gaagacccat gtcgaccgct ccgcggcctt cacgaggtcc cacgcgaggg
    16801 agctcggcct ggacgacagg cgggcggccc gcgtctcccg catcatcctc ggcaccgacc
    16861 tggcccgcca atggagcggg ctggccttcg agagcgagga ggagggcttc ctcgcctcca
    16921 tactcgcggc cgccgacctc ctgggccaga tggccgaccg ggcctacctg gagcgcctcc
    16981 tcttcctgta ctacgaattc cgcgaggccg ggatcgaggg ctacgccacc gccttcgaca
    17041 tcctcaagaa gacggccggc ttctactccg ccgtcaaggc caggctcgac ggccccctgg
    17101 gcggcgcctc cgcctacgcg cggctccact tcgcccgccg ctacggcgag gaccgcgacc
    17161 tctaccgcga ggcgatcgac cggcagatgg cctacctcga cgccatcgtg gccgacgcct
    17221 ccacgaactt ccggaagaag ctccaccggc tcgacctcga ggccgtggag cgggagcgcc
    17281 gggcctagga atcctcctca agcggtcccg gcgggaccgc gaaacggctc ggaggctatg
    17341 cgagctgtcc ggaaagcgcg cctttgcgct tcaacgccga gggcccgcgg gcctcacgaa
    17401 aaagagctca tagtcctcgg tctgccgatc cttcctcggg atcacccgga gttcgccggg
    17461 accggcgagc tcgagggaga gctccgagcc gtccgcgagc gtcgcctcga ggccctttgc
    17521 ggatc
//
LOCUS       DAOOSJ010000019        17270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2268_length_17270_cov_6.202963, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000019 DAOOSJ010000000
VERSION     DAOOSJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_17270_cov_6.202963"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..907
                     /locus_tag="PLG14_02045"
     CDS             161..907
                     /locus_tag="PLG14_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX47940.1"
                     /translation="MSDDARPIYARIADELRAAILARSLGPGDALPSEHELASRYDAS
                     RVTVRKALNVLCSERLVHSRQGKGYFVREPEYSLYSLSFDLIRPGHSVRHRRMEIVEA
                     RGEAAEALSLGEGSPVVHLRLVVLDGPRPLACEDKYLPYRKGFPTIESVTHNAEFPEL
                     VDRKLYRIAMHCDLRLGPAKLDAERARLLGRKEGEPALLARRLIQSDGGRRLGYSLAY
                     LLEEYGELSGSSGYAIYKPAADSKPAAGPG"
     gene            complement(1033..1842)
                     /locus_tag="PLG14_02050"
     CDS             complement(1033..1842)
                     /locus_tag="PLG14_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX47941.1"
                     /translation="MKYRIGTFKRLTGLSEATLRFYDRLGLLCPARDEANDYRSYDEL
                     DLLQLVQVMQYSSFDIPLAELPGEGRSVPAASMLAVLAERRRSIETTISSLYEKLARL
                     KLHETSFERIQEGKPTIAQANIGGIYRLFLSDPAVAEHPAAERIAARWLSYMPYTHAT
                     VRIPLAELQAPGEGPYRSFAGIGMLERYFNERGEKFGEPMQYSPPNTCVHGLVAARDL
                     SSISRQDLAPFFEYLAAHSLIPVGDMFGWVVYIERAGAECRYYLSLRIAVA"
     gene            2224..4278
                     /locus_tag="PLG14_02055"
     CDS             2224..4278
                     /locus_tag="PLG14_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX47942.1"
                     /translation="MRKRIAGALLAAAAIFLAGCASSAGGAKPSDGTFEGSAYGMQGP
                     MKVQISVEGGRIQAIKILESKETPNVVKVALERVPRRIVETQSLKVDSVTGATFASNG
                     IKGAVADAARKAGLDVAAMKAKEAKTKPRAPETWETDVLVIGAGGAGLSAALSAAQTG
                     AKVVLIEKGSVIGGNTMMAGAAYNAVDPEAQATMKLTKAQKAAMDAYLALDPADPALK
                     FDRYPEWKPVLAELKSDIAAFYAANQGKAAGVDMPGFDAVSLHMWHIYTGGLRKMDDG
                     SWIAPRLELARKLAVSALDSLKWMDSIGLKAQYGGTSNYGGKTELSTVLGAMWPRTHT
                     FMSGAERIPQLEKKALEFGVKIYTETRGTELVAGEGGRIVGAKAVQYDGTQVSIKAAK
                     GVVLASGGYCANPAMVKKYDKYWGKDLSDRTLSTNLGTNTGDGVVMAEKLGAQLFGME
                     VAQMMPSSSPTKGTMTDGIWGDAAEQIWIDGQGNRFVDEYAERDVLAKASLKLDRGIF
                     YIVYAGRGNVAKPDVKLRGVEYKAVASMVESGNIWYGETLAELAAASAKSAAGAAPAF
                     TEAALRATIEKYNKYVAEQKDADFGKGVIAGAVDLDYIDATPGVGLCISPRKASLHHT
                     MGGVLIDTEARVLDGEGRPIPGLWAAGEVTGGIHAGNRLGGNAIADIFTFGRIAGLNA
                     AK"
     gene            4453..4920
                     /locus_tag="PLG14_02060"
     CDS             4453..4920
                     /locus_tag="PLG14_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX47943.1"
                     /translation="MSPDRDPATRSRRTFELVEEFLPLFHKCVLPAFHPDLKESLGLS
                     KSQARALMMVRRKGPSTATDIGACLAMTKANLTVLVDELEEAGLVRREDDPGDRRKSL
                     VGITDRGAAAARAIEARIEEALADRLSSLTAAESRRFEDGLASLISVLKTLQE"
     gene            4923..6320
                     /locus_tag="PLG14_02065"
     CDS             4923..6320
                     /locus_tag="PLG14_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX47944.1"
                     /translation="MDRTKELGAAPVPSLLLRFSLPAIVGMLVNAIYNVVDRIFIGQG
                     VGPLGLAGATIAFPLMLVQMAFAMLIGLGASALVSLRLGEGKRDEAERILGNAAGLLV
                     GISLVLSALGLSFLTPLLRLFGASETVLPYARDYSSVILLGTAFQALGMGMNNFIRGE
                     GNPRTAMATMLLGAVLNTVLDPLFIFVFGMGVKGAAIATVISQAASAAWVLAYYLGGK
                     SHLKLRLANLAPRKAIVLPILAIGVAPFSMQIASSVMNSLMNNQLRRYGGDLAVGAMG
                     ILFSVATFFLMPIFGLNQGSQPIVGFNYGAKAYGRVRSAARLAIGAATTFLAAGFALI
                     QLFPGAFVGLFAGSSAELRALGVHAIRIYFLMLPLIGFQVVAASYFQAVGKPRHAMLL
                     SLSRQVLVLIPLILVLPLRFGLEGLWMAPPIADFLSSLLTGFFFLRELRGLTKSEKEG
                     LAGAGTAGAGAAVAG"
     gene            complement(6482..8086)
                     /locus_tag="PLG14_02070"
     CDS             complement(6482..8086)
                     /locus_tag="PLG14_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOX47945.1"
                     /translation="MDTLQSEIRKRLTFAIISHPDAGKTTITEKLLLFGGAIHIAGAV
                     KGGKNARQTTSDFMQMEKERGISISTSVMGFHYKDRCVNLLDTPGHADFSEDTYRGLT
                     AVDSALIVIDSVKGVEERTRRLCEVSRMRDTPIATFINKMDREGKDPISLLDEVEKEL
                     DIQACPMTWPVGQGERFKGVYDLVQKKLYLFRSGGGSELPSDAVLIEDLGNPELDRLV
                     GASLAAKLREDVALLGVVYPAFSLEDYLAGVATPVFFGSAINNFGVRELLDGMVAFAP
                     SPTPKASAEREVSPYEEKLTGFVFKIHANLNPNHRDRIAFLRICSGKFERNKMYLHTR
                     SGKTFRSANPTAFMAQNREIIDEAYAGDIIGIHDTGTFAIGDTITEGESLSYKGIPSF
                     APQIFKVIRNTDPLKEKQFHKGLNQLAEEGVVQILSKMTNPNTRVLGVVGQLQIEVLK
                     SRLEYEYGASCAYDPVDYSIARWFTSKDPKVLKAFIDEHHRRILVDIRGGHILMFESE
                     WQMERVIQKNPEISFHSTSDSMAAAS"
     gene            complement(8238..8954)
                     /locus_tag="PLG14_02075"
     CDS             complement(8238..8954)
                     /locus_tag="PLG14_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX47946.1"
                     /translation="MLRTALFFMYFWLSILLATPFCVLYLLARLLGLGRLLQPAFQAM
                     IRAWAGSLNAVTGSRVRVEGLERIPREGPVCLVGNHQGNWDIVLVLALVPRTVGFIAK
                     SQGLYIPLVNLWIAALDSVFIDRKNVRKAMRSIDRGVGKIRAGKALMVFPEGTRSRGP
                     SMGAFRPGAFKLATRAGAPIVPVTVDGTYRLWEERKRIGRGELLFRVHEPIPTAGIGP
                     EERKLLPERVRAVIEGGLAR"
     gene            complement(9096..11036)
                     /locus_tag="PLG14_02080"
     CDS             complement(9096..11036)
                     /locus_tag="PLG14_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOX47947.1"
                     /translation="MQHEYEATRRAAESSPAESGPAESGPAKPRPARLAGRLALAAFA
                     LAVAGVLAAGLWPDPVERTPSGWKRRESVYVPMRDGVRLAFRVSLPPGLGSGERIPAV
                     FECTRYGTEEKQTFLLKVLLRLRVAKTVHHADFADAANAAGYAFVAVDARGSGASFGT
                     RPMELSREEAEDLGGIVDWIASRPWSNGKVASYGESYSGNTAEVAAASGRPALRAAAP
                     LYPDFDPVMHNAIPFGVLNAALVDGWSASNLRMDANALKGPFFGGMAPVDGPGGGRLL
                     REAVAGHRTIDISAAIRRMTYADDELAPGYSARSLAPYDYKAAIEASGTPLLVRVGWL
                     DAATVNGAIERWLSYSNPQTLVIGPWSHAGWHSKDPFLGGERPTAELEAEEAASVLSF
                     FDAALGERGPRPAGRSIRYYTFGEGLWKETGSWPVPGFEARTLYFGDGGRLSAAPPEA
                     SEGSDRYRVDFSAATGESNRWRTNLGGGAVDYPDRAAEDRKLLCYTGEPLEGDLEITG
                     VPVVTLEVSSTASDGAFFVYLEDVAPDGRVTYLTEGLLRALHRKEGAGDPGRAVLGPA
                     HSFMRRDGEPLELGKPAELRIALQATSVLLRKGHRICVAVAGHDASSFERVPAEGEVE
                     IEVLRSAAFPSRVELPVKARGR"
     gene            11183..12271
                     /locus_tag="PLG14_02085"
     CDS             11183..12271
                     /locus_tag="PLG14_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX47948.1"
                     /translation="MDARFLLDLIGLAVTAPMILAVVLGARRPGARRPFLALVFLEAA
                     LYFLGDILSLSRGTAAAALALNLGSLFYGLPSLYFFAREAAGLAPRRALPHYLPALAA
                     APLGIGLALATAARGFRGGPVLLAYLVLAPLAQTAQYLAYGKAALSLPRRGDGKPDWT
                     RRAVLAVLSGYGLFLLLSWLGLGLLLAAEATGTRPRPLPSVSLSSSLVAVLLAWTLGL
                     CALWGKDAAETRAAAPKYGGRPLPEGEGTRLVERARALLAAEEDLSGEGVEPRRLAAR
                     LGAPYHDLSRAVNELTGASVADLVNEVRIGRAKALLSGRPDLGILDVALESGFAAKST
                     FNEVFRKRVGSSPSEYRKGLAGGDAEKT"
     gene            complement(12268..13674)
                     /locus_tag="PLG14_02090"
     CDS             complement(12268..13674)
                     /locus_tag="PLG14_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HOX47949.1"
                     /translation="MVKNALGRGAVLLALLLALALVLGCSPPLPPLESEEGKAVSVEE
                     LLSDFGSLERSGAKVLVPGGQSFQDLLGLYLVGVKDQFAQYVRSLSAEPPYLVMPVRL
                     RSGGQSGLLWVPVSLGRRLSVPLVSFQHGTQINRNSAPSRFNANPLALLSGPDLVGGL
                     QNYAECLTGALMASAGYIVVMPDYPGFGDSAEPHPYVHLSLGDSVRRMVACAEAVLGG
                     PFAAARPNGRLYLIGYSEGGFATMAAARSLGLAGRVVTAAVPCAGSYDLSGAMLADVL
                     KAEKATIPYYLPYTVFGYASVYEGKAVDGRTDPPGTWAYPRLLKAPLPAWLPQFFGGA
                     ATGSAIAAAIESAFPTGVARDMLTDELLAELAPPAEPSGAVYARLKENTIFHRVDWMP
                     TMPVQPVHCETDDIVPYANAEALHAAYAARGLYNVLPPIKVPPLPMAEDLADIHLRAF
                     PTAMLAGFAFIEGYEARF"
     assembly_gap    14114..14213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14289..15590)
                     /locus_tag="PLG14_02095"
     CDS             complement(14289..15590)
                     /locus_tag="PLG14_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX47950.1"
                     /translation="MADDTVQALRGASIYQVFVRNQSEEGTFEAALPALDKAKELGFD
                     YVYLTPIHPVGAAARKGSLGSPYAIADYRAVDPALGGEEGFRRFLDAAHAKDLRVVMD
                     VVYNHTSPDSVLARERPDWFWKGPEGRPTPRVADWSDVVDLDYGRSGLRDYQIETLEA
                     WFRFGVDGFRCDVASLVPVPFWLEARRRLALAAAAAGREPCLWLAESVHKEFVAELRA
                     RGLYAASDAELHSAFDLSYDYDGRPELEAAWAGRKPLSAYLHHLFLQDCMLPQAAVKL
                     RFLENHDQPRAASLFGRGARLRNWTLFAMLLPGAFLAYAGEESAITRRPSLFDRDPVD
                     WASGDAAFEAWFGEAHQATKGLRAAAPRFSARELAAGLVLLERSGEGRRAAALLNLEG
                     RAGQVALPGPIAGAELLSGARVCLSGTVEIPEEPLLVELDP"
     gene            complement(15613..16485)
                     /locus_tag="PLG14_02100"
     CDS             complement(15613..16485)
                     /locus_tag="PLG14_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX47951.1"
                     /translation="MPLRKRVAGMAALFLAATNFALVILLVKLASRHFSGLFVSGVRF
                     ATGALLCGAVLLFRRGDARLRNPKMVLLRGLFGAASMVASFAAVSLTGPGRAALLGNT
                     YPLFVALFGALFFKERLEARSLLSLAVCTAGALLVMRDGSGAALLGDLLALANALLAG
                     MAVNYVRRASAAGDNPFVLYLSPCLFGLPLLAFAPLPSGPPPASAILLLALVGLGSFS
                     AQVLMSIGYRSVPAGRGSVVFYWETALTVLLGFLFGGESPNPRFLLGLCAILAGLWLN
                     GGRPRGAKAAAAGR"
     gene            16834..>17270
                     /locus_tag="PLG14_02105"
     CDS             16834..>17270
                     /locus_tag="PLG14_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47952.1"
                     /translation="MDRVRPGRLILAAVLPFLLAACPEAITPGIVDGARDATPPVISV
                     ASPAEYASYARTILIQGSVADLAGPGKAGRVEALSYEILSHTAPKPAALASDGSFLIS
                     EPNDLRENIVVLLKATDWNGNEAEYRLPLTYPGNEIPSFAAAEG"
BASE COUNT         2568 a         5999 c         6101 g         2502 t
ORIGIN      
        1 tccgaaggct tcgaggcgcc ttccccagca tgggagggct cgcgctccgc ggcggcgagg
       61 ccctcgccac ggccttcgcg gagctcgggg ccgacgccta cgccgaggac atcctcgcgg
      121 cccaggactt ctgcctcgcc cgggcgaggg gagcccgatc atgagcgacg atgccaggcc
      181 catctacgcg cgcatcgcgg acgagctccg ggccgcgatc ctggcgcgga gcctcggccc
      241 gggcgacgcg ctgccgagcg agcacgagct ggcctcgcgc tacgacgcca gccgcgtcac
      301 cgtgcgcaag gccctgaacg tgctgtgctc cgagcgcctg gtccactccc gccagggcaa
      361 gggctacttc gtgcgcgaac ccgagtacag cctgtactcc ctgtccttcg accttatccg
      421 gcccgggcac tccgtccggc atcgccggat ggagatcgtc gaagcccggg gcgaggcggc
      481 cgaagccctg tccctgggcg aggggagccc cgtcgtccac ctgcggctcg tggtcctcga
      541 cggcccgcgg cccctcgcct gcgaggacaa gtaccttccg tacaggaagg gcttcccgac
      601 gatcgagagc gtgacgcaca acgcggagtt ccccgagctg gtcgaccgca agctctaccg
      661 catcgccatg cactgcgacc tgcgcctcgg gcccgcgaag ctcgacgcgg agcgcgcgcg
      721 gctgctggga aggaaggagg gcgagcccgc cctcctcgcg aggcggctga tccagagcga
      781 cggcggcagg aggctcggct acagccttgc ctacctgctc gaggagtacg gggagctctc
      841 gggatcctcg ggctacgcga tctacaagcc cgccgcggac tcgaagccgg ccgccgggcc
      901 gggctgatcc gcccggccct cgcctcggtc cgggagcggc cggcgggaag cctatcggac
      961 cggctggccg tagtacgcgc cggcccccgc tccatgccga ccccgcaggg acctgccgcc
     1021 aagacctcgg gctcaggcca cggctatgcg caggctgagg tagtacctgc actcggcccc
     1081 ggcgcgctcg atgtagacga cccacccgaa catgtcgccg acgggaatga gggaatgcgc
     1141 cgcgagatat tcgaagaagg gcgcgaggtc ctggcgcgag atggaggaca ggtcccgcgc
     1201 cgcgacaagg ccgtggacgc aggtgttggg cggcgagtac tgcatgggct cgccgaactt
     1261 ctctccccgc tcgttgaagt accgctcgag catgcctatc cccgcgaagc ttcggtaggg
     1321 gccttcgccg ggagcctgga gctcggcgag ggggatgcga accgtggcgt gcgtgtaggg
     1381 catgtacgag agccagcgcg cggcgatccg ctccgcggcg ggatgctccg cgaccgcggg
     1441 atctgagagg aagagccggt agattccgcc gatgttcgcc tgcgcgatcg tcggcttccc
     1501 ttcctggatc cgctcgaagg aggtctcgtg cagcttgagc ctggccagct tctcgtagag
     1561 cgaggagatg gtcgtctcga tgcttctccg tcgctcggcg aggacggcca gcatgctcgc
     1621 cgcgggcacc gaccggccct cgccgggcag ctccgccaga ggtatgtcga agctgctgta
     1681 ctgcatgacc tgcacgagct ggagcaggtc gagctcgtcg tagctccggt agtcgttggc
     1741 ctcgtccctg gccgggcaga ggaggccaag cctgtcgtag aagcggagcg tggcctcgga
     1801 gagtcccgtc aatcgcttga aagtcccgat ccggtatttc acccctctcc ctccgtcatc
     1861 ctccgcgctt gggccactat agcacggcga aggcgcggcg cagaggacct tgaagccatc
     1921 tcgaggcttt tttagcgaat ctcggcgggc gggaaggccc gcaagcggcc attagaggag
     1981 aagaacaagg cccatggggc tgtgtcgcgc gggatcgacg gccaggcgcg ccccatacat
     2041 gcccggcaga cctaggttcg tgccccttgc ccgcggaatg gcgggcgttc ggagccccgg
     2101 cccgcggagc ggcggccccc caagcccccg atccgcctct tcgccgactt cccgattgag
     2161 gttgaccctc aagttcggtc gaggtttagg ctttcggcat acgcgattca tcaaggagga
     2221 atcatgagaa agagaatagc cggcgccttg ctggccgccg cggcgatctt cctcgccggc
     2281 tgcgcctcga gcgccggagg ggccaagccc tccgacggaa ccttcgaggg cagcgcctac
     2341 gggatgcagg ggccgatgaa ggtccagatc tccgtggaag gcggccgcat ccaggcgatc
     2401 aagatcctcg agtccaagga gacccccaac gtcgtgaagg tcgccctgga gcgggtcccc
     2461 cgccgcatcg tcgagaccca gagcctcaag gtcgactcgg tcaccggcgc caccttcgcc
     2521 tccaacggca tcaagggcgc cgtcgccgac gcggccagga aagccggcct ggacgtggcc
     2581 gcgatgaagg ccaaggaggc caagacgaag cccagggctc ccgagacctg ggagaccgac
     2641 gtcctcgtca tcggcgccgg cggcgcgggc ctctccgcgg ccctgagcgc ggcccagacg
     2701 ggcgccaagg tcgtcctcat cgagaagggc tccgtgatcg gcggcaacac catgatggcc
     2761 ggcgccgcct acaacgcggt cgaccccgag gcccaggcca ccatgaagct gaccaaggcc
     2821 cagaaggccg cgatggacgc ctacctcgcc ctggaccccg cggatccggc gctcaagttc
     2881 gaccgctatc cggagtggaa gcctgtcctc gccgagctca agtccgacat cgccgccttc
     2941 tacgccgcca accagggcaa ggccgccggc gtcgacatgc ccggcttcga cgccgtctcc
     3001 ctccacatgt ggcacatcta cacaggcggc ctcaggaaga tggacgacgg cagctggatc
     3061 gcgcccaggc tggagctggc gcgcaagctc gccgtgagcg cccttgacag cctcaagtgg
     3121 atggacagca tcgggctcaa ggcgcagtac ggcgggacct ccaactacgg cgggaagacg
     3181 gagctctcca ccgtcctggg cgcgatgtgg ccccgcaccc acaccttcat gtccggcgcg
     3241 gagcgcatcc cccagctcga gaagaaggcc ctggagttcg gcgtgaagat ctacaccgag
     3301 acccgcggca ccgagctcgt cgccggcgag ggcgggagga tcgtcggcgc caaggcggtc
     3361 cagtacgacg gcacgcaggt ctccatcaag gcggccaagg gcgtcgtcct cgcctccggc
     3421 ggctactgcg ccaacccggc catggtgaag aagtacgaca agtactgggg caaggacctc
     3481 tccgacagga ccctctccac caacctgggc accaacaccg gcgacggcgt cgtcatggcc
     3541 gagaagctcg gagcccagct cttcggcatg gaggtcgcac agatgatgcc ctcctcctcc
     3601 cccaccaagg gcacgatgac cgacggcatc tggggcgacg cggccgagca gatctggatc
     3661 gacggccagg gaaaccgctt cgtggacgag tacgccgagc gcgacgtcct cgcgaaggcc
     3721 tcgctcaagc tcgacagggg gatcttctac atcgtctacg ccggccgcgg caacgtcgcc
     3781 aagcccgacg tcaagctcag gggcgtggag tacaaggccg tcgcgtccat ggtcgagtcg
     3841 ggcaacatct ggtacggcga gaccctcgcc gagctggccg cggcgtcggc gaagagcgcg
     3901 gccggcgccg cccccgcctt caccgaggcc gccctccgcg cgaccatcga gaagtacaac
     3961 aagtacgtcg ccgagcagaa ggacgccgac ttcggcaagg gcgtgatcgc cggcgccgtc
     4021 gacctcgact acatcgacgc cacgcccggc gtgggcctct gcatcagccc gcgcaaggcc
     4081 tccctccacc acacgatggg cggcgtcctc atcgacaccg aggcccgcgt cctcgacggc
     4141 gagggccggc ccatccccgg cctctgggcc gccggcgagg tgacgggcgg catccacgcg
     4201 ggcaaccgcc tgggcggcaa cgcgatcgcc gacatcttca ccttcggccg catcgcgggc
     4261 ctcaacgccg cgaagtgacc ttctccgccc ggtccgccgc gctgcgcggc ggaccggggg
     4321 caggccccgc tccggtcttg ccggggcggg gccttttcat gccgccatat gcggcccggc
     4381 cttgggcccc tggacatgaa gccgtttttt agttaatttt atgactgttc aattttatac
     4441 taaggtggag ccatgagtcc cgatagagac ccggcgaccc ggtcccgcag gactttcgaa
     4501 ctcgtcgagg agttcctccc cctgtttcat aagtgcgtcc tgcccgcctt ccaccccgac
     4561 ctcaaggaaa gcctcggcct ctcgaagagc caggcgcggg ccctgatgat ggtgcgccgc
     4621 aaggggcctt cgaccgcgac ggacatcggc gcctgcctgg ccatgaccaa ggccaacctc
     4681 accgtcctcg tggacgagct cgaggaggcg ggcctggtcc gcagggagga cgacccgggg
     4741 gacaggcgga agagcctcgt gggaatcacc gacaggggcg cggccgccgc ccgcgccata
     4801 gaggcccgca tcgaggaggc gctcgccgat cggctctcga gcctcaccgc ggccgagtcg
     4861 aggcgcttcg aggacggcct cgcgagcctc atctccgtct tgaagaccct acaggagtga
     4921 ccatggaccg aaccaaggaa ctgggcgccg cgcccgtccc ttccctgctc ttgcgctttt
     4981 ccctgcccgc catcgtgggc atgctcgtca acgcgatcta caacgtcgtc gaccggatct
     5041 tcatcggcca gggcgtcgga cccctgggcc tcgcgggcgc gacgatcgcc ttccccctca
     5101 tgctcgtgca gatggccttc gcgatgctca tcggcctcgg cgcctcggcc ctcgtctccc
     5161 tgcggctcgg cgagggaaag agggacgagg ccgagcgcat cctcgggaac gcggccggcc
     5221 tgctggtcgg catctccctc gtcctttccg cgctgggcct ctccttcctc acgcccctac
     5281 tcaggctctt cggcgcgagc gagacggtcc tgccctacgc ccgcgactac tcctccgtga
     5341 tcctcctcgg cacggccttc caggcgctgg ggatgggcat gaacaacttc atccgcggcg
     5401 agggcaaccc gcggacggcg atggcgacca tgctcctcgg agccgtcctc aacaccgtcc
     5461 tcgatcccct cttcatcttc gtcttcggca tgggcgtgaa gggcgcggcc atcgccacgg
     5521 tcatctcgca ggctgcctcc gccgcctggg tgctcgccta ctacctgggc ggcaagagcc
     5581 acctcaagct tcgcctggcc aacctcgcgc cgcgcaaggc tatcgtgctc ccgatcctcg
     5641 cgatcggcgt cgcgcccttc tccatgcaga tcgcctcatc cgtcatgaac agcctgatga
     5701 acaaccagct caggcgctac ggcggggact tggcggtggg cgccatgggc atcctcttct
     5761 cggtcgccac cttcttcctc atgcccatct tcggcctgaa ccagggctcc cagcccatcg
     5821 tcggcttcaa ctacggggcc aaagcctacg gacgggtgcg ctccgccgcg cgcctcgcca
     5881 tcggggccgc cacgaccttc ctggccgcgg gcttcgccct catccagctc ttccccggcg
     5941 ccttcgtggg gctcttcgcg ggctcctccg cggagctcag ggccctgggc gtccacgcga
     6001 tcaggatcta cttcctcatg ctccccctga tcggcttcca ggtcgtcgcg gcgagctact
     6061 tccaggcggt gggcaagccc cgccacgcga tgctcctgtc cctctcgcgg caggtcctcg
     6121 tcctcatccc cctcatcctc gtcctcccct tgcgcttcgg gctcgagggg ctctggatgg
     6181 cgccgcccat cgcggacttc ctctcctccc tactcacggg cttcttcttc ctccgcgagc
     6241 tccgcggcct gacgaagtcg gagaaagaag gcctggccgg cgccggaacg gccggcgcag
     6301 gggcggccgt cgcgggctag tctcgctccg gcctcgggcc ggcttccccg aaaacagaga
     6361 gagggccatc ccgcgcggga tggccctctc ttttccttcg ccccggcacg cggctcggaa
     6421 gagccgcggc cgggcgccga acccaggcgc ggcgccgccg gcctggcgcg ccccggctac
     6481 gctaggaggc ggccgccatc gagtcggagg tgctgtggaa gctgatctcc gggttcttct
     6541 ggatgacgcg ctccatctgc cactcggact cgaacatgag gatgtggccg ccccggatgt
     6601 cgacgaggat ccgccggtgg tgctcgtcga tgaaggcctt gaggaccttg gggtccttgg
     6661 aggtgaacca gcgggcgatc gagtagtcga ccgggtcgta ggcgcaggag gccccgtact
     6721 cgtactcgag ccgggacttg agcacctcga tctggagctg gcccacgacg cccaggacgc
     6781 gcgtgttggg gttcgtcatc ttggagagta tctggacgac gccctcctcc gcgagctggt
     6841 tcaggccctt gtggaactgc ttctccttca gggggtcggt gttcctgatg accttgaaga
     6901 tctgcggagc gaagctcggg atgcccttgt agctcaggct ctcgccctcg gtgatggtgt
     6961 cgcctatcgc gaaggtgccc gtgtcgtgga tgccgatgat gtcgcccgcg taggcctcgt
     7021 cgatgatctc ccggttctgg gccatgaagg ccgtcgggtt ggcgctacgg aaggtcttgc
     7081 cgctccgcgt gtgcaggtac atcttgttgc gctcgaactt cccggagcag atcctgagga
     7141 aggcgatccg gtcgcggtgg ttcgggttca ggttcgcgtg gatcttgaag acgaagcccg
     7201 tgagcttctc ctcgtaaggg gagacctcgc gctcggcgga ggccttgggc gtcggcgagg
     7261 gcgcgaaggc caccatgccg tccaggagct cgcgcacgcc gaagttgttg atggcggagc
     7321 cgaagaagac cggcgtcgcg acgcccgcga ggtagtcctc caggctgaag gccgggtaga
     7381 cgaccccgag gagggcgacg tcctcgcgca gcttcgcggc gaggctcgcg ccgacgagcc
     7441 tgtccagctc ggggttgccg aggtcctcga tcagcacggc gtcggagggc aactcgctgc
     7501 cgccgcccga cctgaagagg tagagtttct tctggacgag gtcgtagacg cccttgaagc
     7561 gctcgccctg gccgacgggc caggtcatcg ggcaggcctg gatgtccagc tccttctcga
     7621 cctcgtccag gagggagatc gggtccttgc cctcgcggtc catcttgttg atgaaggtgg
     7681 cgatgggggt gtcgcgcatc ctggacacct cgcagaggcg gcgggtgcgc tcctccacgc
     7741 ccttcacgga gtcgatgacg atgagggccg agtcgacggc ggtgaggccc cggtaggtgt
     7801 cctccgagaa gtcggcgtgg cccggcgtgt cgaggaggtt gacgcagcgg tccttgtagt
     7861 ggaagcccat caccgaggtg gagatggaga tgccgcgctc cttctccatc tgcatgaagt
     7921 cggaggtggt ctgcctggcg ttcttgccgc ccttgaccgc gccggcgatg tggatggccc
     7981 ctccgaagag gaggagcttc tcggtgatgg tcgtcttgcc cgcgtcgggg tgcgagatta
     8041 tggcgaaggt gagccgcttg cggatctcgc tctgtagtgt atccatggat gggcaagcgt
     8101 accggcgggc attttgagtg tcaaggacgg cgctccgcgc cctctggagg gcgctcgggc
     8161 cgcaagccac ctccattcag gccgcgcggc cgctgccccc ccgccagagg gcggcctgca
     8221 agcccggcgc gggccgccta gcgggccagg ccgccttcta tgacggcgcg cacccgctcg
     8281 gggaggagct tcctctcctc ggggccgatg ccggccgtgg ggatcggctc gtggacccgg
     8341 aagaggagct cgccacggcc gatccgcttc ctctcctccc acagcctgta ggtcccgtcg
     8401 acggtcaccg gcacgatcgg cgcgccggcc ctggtggcga gcttgaaggc gccgggcctg
     8461 aaggcgccca tggagggtcc cctgctgcgc gtgccctcgg ggaagaccat gagggccttg
     8521 ccggcgcgga tcttcccgac gccgcggtcg atggagcgca tggccttgcg cacattcttg
     8581 cggtcgatga agacggagtc gagggcggcg atccagaggt tcacgagggg gatgtagagc
     8641 ccctggctct tcgcgatgaa gccgacggtg cggggcacga gggcgaggac gaggacgatg
     8701 tcccaattgc cctggtggtt tccgacgagg cagacggggc cctcgcgggg gatccgctca
     8761 agcccctcga cgcggactcg cgagcccgtg accgcgttca ggctccccgc ccaggcgcgg
     8821 atcatggcct ggaaggccgg ctggaggagc cggcccaggc cgaggagcct cgcgaggagg
     8881 tagaggacgc agaagggcgt cgcgaggagg atcgaaagcc agaagtacat gaagaacagc
     8941 gccgtccgca gcatggccca tcattgccgg atcggggctt cgcggcaatc ccccttgcgc
     9001 gaggccggct ctcggccgcg gccggccgcg gccgagagcc gcggaagctg gcctgaccgg
     9061 cggcggccaa tccgactggc cggccccggc cgccttcagc ggccgcgcgc cttgactggc
     9121 agctcgacgc gcgacgggaa ggcggcgctc ctcaagactt cgatctccac ctcgccttcg
     9181 gcggggacgc gctcgaagga ggaagcgtcg tggccggcga cggcgacgca gatgcggtgc
     9241 cccttgcgga ggaggaccga ggtggcctgc agggctatcc tgagctcggc gggcttcccc
     9301 agctccaggg gctcgccgtc gcgccgcatg aagctgtggg cgggtccgag gacggcgcgc
     9361 ccgggatcgc cggcgccctc cttgcggtgg agggcgcgga gcaggccctc ggtgaggtag
     9421 gtgaccctgc cgtcaggcgc gacgtcctcc aggtagacga agaaggcgcc gtcgctcgcg
     9481 gtggaggaga cctcgagggt caccaccggg acgcccgtga tctccaggtc gccctccagg
     9541 ggctcgccgg tgtagcagag gagcttcctg tcctccgcgg cgcggtcggg gtagtcgacg
     9601 gccccgccgc ccaggttcgt gcgccagcgg ttcgactctc ccgtggccgc ggaaaagtcg
     9661 acgcggtagc ggtccgagcc ctccgacgcc tcgggcggcg ccgccgacag gcggccgccg
     9721 tccccgaagt agagggtcct cgcctcgaag cccgggaccg gccaggagcc ggtctccttc
     9781 cagagcccct cgccgaaggt gtagtagcgg atcgacctgc ccgcggggcg cgggccccgc
     9841 tcgcccaggg cggcgtcgaa gaaggaaagg acggaggcgg cctcctcggc ctcgagctcg
     9901 gccgtgggcc gctctccgcc caggaagggg tccttcgagt gccagcccgc gtgggaccag
     9961 ggcccgatga cgagggtctg cgggttcgag tagctgagcc agcgctcgat ggcgccgttg
    10021 accgtcgccg cgtccagcca gccgacgcgg acgaggaggg gcgtccccga ggcctcgatg
    10081 gccgccttgt agtcgtaggg cgcgagggag cgggccgagt agccgggcgc gagctcgtcg
    10141 tccgcgtagg tcatccgccg gatggcggcg gatatgtcga tggtcctgtg cccggcgacg
    10201 gcctcgcgga ggaggcgccc gccgcccggg ccgtcgacgg gcgccatgcc tccgaagaag
    10261 gggcccttga gggcgttcgc gtccatcctc aggttcgagg cgctccagcc gtccacgagg
    10321 gcggcgttga ggacgccgaa gggtatcgcg ttgtgcatga ccggatcgaa gtccggatag
    10381 aggggcgccg ccgcgcgcag ggccggccgg ccggaggccg ccgcgacttc cgcggtgttg
    10441 cccgagtagg actcgccgta ggaggcgacc ttcccgttgg accagggccg ggaggcgatc
    10501 cagtcgacga tccctcccag gtcctccgcc tcctcgcggg agagctccat gggccttgtc
    10561 ccgaaggagg cgcccgagcc gcgcgcgtcg acggcgacga aggcgtagcc cgccgcgttg
    10621 gccgcgtcgg cgaaatccgc gtggtggacg gtcttcgcga ccctcaggcg gaggaggacc
    10681 ttgaggagga aggtctgctt ctcctcggtg ccgtagcggg tgcattcgaa caccgcgggt
    10741 atcctctcgc ctgatccaag tcccggaggg agggagacgc ggaaggcgag gcggacgccg
    10801 tcccgcatcg ggacgtagac cgactcccgg cgcttccagc ccgagggcgt ccgctccacg
    10861 ggatcgggcc agaggcctgc cgccaggacg ccggcgacgg cgagggcgaa ggccgcgagg
    10921 gcgaggcggc cggcgagcct ggccgggcgg ggcttggccg ggccggactc ggccgggccg
    10981 gactcggccg ggctggactc ggccgcgcgg cgcgtggctt cgtattcgtg ctgcatgatc
    11041 gggcatctcc ttgcgaggct ctcatcgagc cgtggcccga gaatgcccct tccagggccg
    11101 ccggtcgtcc gaagcgatcg gatcggacgg aaattcggca aattcgcttt cgcgggccct
    11161 ccccgtggtt tatcgtgggc ccatggatgc ccggtttctt ctcgacctca tcggcctggc
    11221 cgtcacggcc ccgatgatcc tcgccgtcgt cctgggcgcg aggcggcccg gcgcgcgccg
    11281 tcccttcctg gccctcgtct tcctggaggc cgccctctac ttcctgggcg acatcctctc
    11341 cctctccagg ggaacggccg cggccgccct cgccctcaac ctcgggagcc tcttctacgg
    11401 cctcccctcg ctctacttct tcgcccgcga ggccgcgggc ctagccccca ggcgggccct
    11461 cccccactac cttcccgcgc tcgcggcggc tcctctgggg atcggcctcg ccctcgccac
    11521 cgcggcccgc ggcttcaggg gagggccggt cctcctcgcc tacctggtcc tcgcgccgct
    11581 cgcccagacg gcccagtacc tggcctacgg caaggcagcc ctctccctgc cgcggcgggg
    11641 cgacgggaag ccggactgga cgaggagggc cgtgctcgcc gtcctctctg gctacggcct
    11701 cttcctcctc ctctcctggc tcggcctcgg ccttctcctc gccgccgaag ccacgggtac
    11761 gaggccgcgc cccctcccct ccgtctccct ctcctcgagc ctcgtcgccg tcctcctcgc
    11821 ctggaccctg ggcctctgcg cgctctgggg caaggacgcg gcggagacca gggccgcggc
    11881 gcccaagtac ggcggccggc ccctgcccga gggcgaggga acacgcctcg tcgagagggc
    11941 gcgcgccctc ctcgccgcgg aggaggacct gtcgggcgag ggcgtcgagc cccgccgcct
    12001 cgccgcccgg ctcggcgcgc cctaccacga cctctcccgc gcggtgaacg aactcaccgg
    12061 agcgagcgtc gccgacctcg tcaacgaagt caggatcggg cgcgccaagg ccctcctctc
    12121 tggccggccg gacctgggca tcctcgacgt tgccctggag tcgggcttcg ccgcgaagtc
    12181 gaccttcaac gaggtgttca ggaagcgcgt cggctcgagc ccgagcgagt accgcaaggg
    12241 cctcgccggg ggcgacgccg aaaaaaccta gaaccgggcc tcgtagccct cgatgaaggc
    12301 gaagcccgcg agcatcgccg tcgggaaggc cctgaggtgg atgtccgcga gatcctcggc
    12361 catcggcaag ggcgggacct tgatcggcgg cagcacgttg tagaggcccc tcgccgcgta
    12421 ggccgcgtgg agcgcctccg cgttcgcgta gggcacgatg tcgtcggtct cgcagtggac
    12481 gggctggacg ggcatcgtgg gcatccagtc gacgcggtgg aagatcgtgt tctccttgag
    12541 ccgcgcgtag acggcgcccg aaggctcggc cggcggcgcg agctcggcga gcagctcgtc
    12601 ggtgagcatg tcgcgagcga ccccggtcgg gaaggccgac tcgatggccg cggcgatggc
    12661 cgaacccgtc gcggccccgc cgaagaactg cggaagccag gccgggagcg gcgccttgag
    12721 gagccttggg taggcccagg tgcccggcgg atcggtccgc ccgtcgacgg ccttgccctc
    12781 gtagacggag gcgtagccga agaccgtgta gggcaggtag taggggatcg tggccttttc
    12841 cgccttgagg acgtccgcga gcatggcgcc cgaaaggtcg taggagccgg cgcagggcac
    12901 cgcggcggtg acgacgcggc ccgcgaggcc gaggctcctg gccgcggcca tcgtcgcgaa
    12961 gccgccctcg gagtagccga tgaggtagag ccgcccgttg ggccgcgcgg ccgcgaaggg
    13021 gccgcccaac acggcctccg cgcaggcgac catgcggcgg acggagtcgc ccagggagag
    13081 gtggacgtag ggatggggct cggcgctgtc gccgaagccc gggtagtcgg gcatcacgac
    13141 gatgtagccc gcggaggcca tgagggctcc cgtcaggcac tcggcgtagt tctgcaggcc
    13201 gccgaccagg tcggggccgg agagcagcgc cagggggttc gcgttgaagc gcgaaggcgc
    13261 cgagttcctg ttgatctggg ttccgtgctg gaaggagacg aggggcacgg agagcctccg
    13321 gcccaggctg acgggcaccc agaggaggcc gctctggccg cccgagcgga gcctcacggg
    13381 catcacgaga tacggcggct cggcggagag agagcggacg tactgggcga actggtcctt
    13441 gacgccgacc aggtagaggc cgagcaggtc ttggaaggac tggccgccgg gcacgaggac
    13501 cttggcgccg ctccgttcca ggcttccgaa gtccgagagg agctcctcga cggagaccgc
    13561 cttgccctcc tcggactcga ggggcggcaa gggcggcgag cagccgagga caagggccag
    13621 ggcgaggagg agggccagga ggaccgcccc ccgaccgagc gcgtttttga ccatgggaac
    13681 ctcctatcgg gcgcgcgatt ccgcgcccgg acaaagaaag gctccgctcc gccccccgct
    13741 tcagggcgag ggcttttcgg ggcgggtttg tcgcttcgtt gtcgcttgct ttcggaggcg
    13801 ggactatcgg gcggcttgcc gagacggtgc caggcgggaa gggctctgcg ggcgcaaggt
    13861 ggggtggaac ggttgctcga tagaggcacc gactcttcaa gcattccgca ttccccggaa
    13921 atacgcaagg ccggcggggt tttccgcgcg aagcggccgg cccgcgcggc gccaaggctt
    13981 cgcctcgcgc cgccgtcttt gcgcgccggg tcgcgccgcc actacgccgc tgcctggcgc
    14041 gcggctcagc caacctgcgc ggcgcggccc gcccgcgcgg ctcgacgcgg cctgcccggc
    14101 tatcgcggcg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggcgc
    14221 ggcccgcccg cgcggctcga cgcggcctgc ccggctatcg cggcgcggcc ggcccgcgca
    14281 gcgcggccct aagggtcgag ctcgacgagg aggggttcct cggggatctc gaccgtcccc
    14341 gagaggcaga cgcgggcgcc cgagaggagc tccgcgccgg cgatcggccc gggaagggcg
    14401 acctgccccg cgcggccctc caggttgagg agggcggccg ccctcctccc ctcgcccgag
    14461 cgctcgagga ggacgaggcc ggccgcgagc tcgcgggcgg agaagcgcgg ggcggccgcg
    14521 cgcaagccct tcgtcgcctg gtgcgcctcg ccgaaccagg cctcgaaggc cgcgtcgcct
    14581 gaggcccagt cgacgggatc cctgtcgaag aggctcggcc tccgcgtgat cgccgactcc
    14641 tcccccgcgt aggcgaggaa ggccccgggc aggagcatcg cgaagagggt ccagttcctg
    14701 agccgcgcgc cgcggccgaa gagggaggcg gcccggggct ggtcgtggtt ctccaggaag
    14761 cggagcttga ccgccgcctg gggcagcatg cagtcctgga ggaagaggtg gtggagatag
    14821 gcggagaggg gcttccggcc ggcccaggcc gcctccagct cggggcggcc gtcgtagtcg
    14881 taggagagat cgaaggccga gtggagctcg gcgtccgagg ccgcgtagag ccccctcgcc
    14941 ctgagctcgg cgacgaattc cttgtggacg ctctccgcga gccagaggca gggttcccgg
    15001 cccgccgccg cggcggccag ggcgagccgg cgtcgagcct cgagccagaa cgggacgggc
    15061 acgaggctcg ccacgtcgca gcggaagccg tcgacgccga agcggaacca ggcctccagg
    15121 gtctctatct ggtagtcccg gagcccggac cgcccgtagt ccaggtccac gacgtcggac
    15181 cagtcggcca ccctcggcgt cggcctgccc tcaggtccct tccagaacca gtccggccgc
    15241 tcgcgggcca ggaccgagtc ggggctcgtg tggttgtaga ccacgtccat gacgacacgg
    15301 aggtccttcg cgtgggcggc gtccaggaaa cgcctgaagc cctcctcgcc gcccagggcc
    15361 gggtcgacgg cgcggtagtc cgcgatcgcg taggggctcc cgaggctgcc cttcctcgcg
    15421 gccgcgccga cggggtggat gggcgtgagg tagacgtagt cgaagccgag ctccttcgcc
    15481 ttgtccaggg cgggcagggc ggcctcgaag gtcccctcct ccgactggtt gcggacgaag
    15541 acctggtaga tcgaggcgcc ccggagggcc tggaccgtat cgtcggccat ggagtctcct
    15601 ttggcggcga gtctatcggc cggcggccgc ggccttcgcg ccgcggggcc gcccgccgtt
    15661 gagccagagc cccgcgagta tcgcgcagag gcccaggagg aagcgcgggt tcgggctctc
    15721 cccgccgaag aggaagccga ggaggacggt gagggccgtc tcccagtaga agaccacgga
    15781 accccgcccc gccggcacgc tcctgtagcc tatcgacatg aggacctggg cgctgaagga
    15841 gcccaggccc acgagggcga ggaggaggat cgcggaggcc gggggcgggc ccgaggggag
    15901 cggcgcgaag gcgaggaggg gcaggccgaa gaggcagggc gagaggtaga ggacgaaggg
    15961 attgtcgccc gcggcggagg cgcggcggac gtagttcacg gccatgcccg cgaggagggc
    16021 gttcgcgagg gcgaggaggt cgccaaggag ggcggccccg ctgccgtcgc gcatgacaag
    16081 gagagctccc gcggtgcaga cggccaggga aaggagactc ctcgcctcga gccgctcctt
    16141 gaagaagaga gcgccgaaga gggccacgaa gagggggtag gtgttgccga ggagagcggc
    16201 ccttcccggc cccgtgaggg agacggccgc gaaggaggcg accatggagg ccgcgccgaa
    16261 gaggccgcgc aggaggacca tcttcggatt cctgaggcgg gcatcgccgc gccggaagag
    16321 gaggacggcg ccgcagagga gggctcccgt cgcgaagcgg accccggaga cgaagaggcc
    16381 cgagaaatgg cgcgaggcga gctttacgag gaggatgacg agggcgaagt tggtcgcggc
    16441 gaggaagagg gcggccatcc cggcgaccct tttgcgtagg ggcatgggcc atcctacccg
    16501 gcccgcggct cggtcggcta cggctggcgc ggccgaggga gccgtctcgc cgcggccacc
    16561 tcggcaggcg gcgctgaacc gcggccccgc cacacgatgc catgccgggc cacgcggtgc
    16621 cgccgcgccg ccgggccgcg ccgggccacc tcgccgcagg accatgcagg gccaccgggc
    16681 cgcgcctgag ccgaggtctg ggacgccggg ccacgccgac acgccgcccg ggcgctctcg
    16741 atttccgccg ccccaaataa ataataatct ctatattaga ttattacatt ttttgtcagt
    16801 atatttattg tacttccagc aaggagggat gtcatggaca gggttcgacc aggacgcttg
    16861 atcctcgccg cggtgcttcc cttccttctc gcggcctgtc ccgaggcgat cacgcccggc
    16921 atcgtcgacg gcgcccgcga cgcgacccct cccgtcatca gcgtggcgag ccccgcggag
    16981 tacgcgagct acgcccgcac catcctgatc cagggaagcg tcgccgacct cgcgggacca
    17041 ggcaaggcgg gccgcgtcga ggcgctcagc tacgagatcc tctcgcacac cgcgccgaag
    17101 cccgccgccc tcgcctccga cggatccttc ctcatctccg agcccaacga cctgcgggag
    17161 aacatcgtcg tcctcctcaa ggccacggac tggaacggca acgaggcgga gtaccgcctc
    17221 cccctcacct acccggggaa cgagatcccg agcttcgccg ccgcggaggg
//
LOCUS       DAOOSJ010000020        17263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2270_length_17263_cov_5.681834, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000020 DAOOSJ010000000
VERSION     DAOOSJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_17263_cov_5.681834"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLG14_02110"
     CDS             complement(<1..558)
                     /locus_tag="PLG14_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47953.1"
                     /translation="MKRILIVAALVACALAVGASSVWEGSAVQGGAGDFPAEGLYGSC
                     NSFPKNSRVEVENLGNGKKAVVTITGTVGNPGVFMALSPSAARELGMKAGDAARVRVL
                     AEPAASVPAPALPARPGESSDPDFNPKLLALRERSAPASSSAKTAAPVQPAAPAAAAP
                     ASAAPADVALLLKPAEPPLPEKAETA"
     gene            complement(558..1085)
                     /locus_tag="PLG14_02115"
     CDS             complement(558..1085)
                     /locus_tag="PLG14_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47954.1"
                     /translation="MRRAQALALAAVLAALGMAGKASAQTVAVLAAESVDPPAGEATE
                     AVVSGLMDRLFDSGLIATSERTRQVERADWRLEPRSLGDAREGSVDYLACVYVAYAPS
                     ALRPGLPRPLGLELRLYRVADGGLAAEASLPPFAGLDETEEGLSRLLRALGGEAAEAL
                     MNALRQGRPGGSGGV"
     gene            1252..1800
                     /gene="loaP"
                     /locus_tag="PLG14_02120"
     CDS             1252..1800
                     /gene="loaP"
                     /locus_tag="PLG14_02120"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HOX47955.1"
                     /translation="MEYFVLQVKSNEEDEFIRRASVSPAGCHGRLFSPKRVLPIRRGG
                     KVTNRELPVFPGYVFLEVDSLDREARWDFRHVEGFYRFLRDAEGDPVPLSEKDRSLLL
                     HFISLGKRADISKVTFDEKDRIVVIEGALKGLEGRIAKVDRRKGRARVILDLYEKSFP
                     IDFGFEEVRKVGQGGASSHDES"
     gene            1787..3277
                     /locus_tag="PLG14_02125"
     CDS             1787..3277
                     /locus_tag="PLG14_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOX47956.1"
                     /translation="MTSPERENARVYIIGAGFAGRAIAREILAKGVIGQVVAFLDDNP
                     AKIGGRIEGVPVLGPISDVVSLLSRMPADEAIIAMPSAPRERLRELYFLLKRAGFARI
                     RIVPGVAQILEGDAHLVQAREIDPQDFLGRNPVAIGLKESLSYLRGKRVLITGAGGSI
                     GSELARQLLSAGVARLYLFGHGENSIYQIDRELRLLQAEGVGERTAVVPVIGELKDPD
                     YVRFVLDRLKADAVFHAAAYKHVPMMEENPIAAVENNVFGTRNLVEAALAAGVKRLVL
                     ISTDKAVEPACVYGASKFLSERIVEAAAARAAPGQSFMVVRFGNVLGSRGSIVPLFKK
                     QIEQGGPVTVTHPETSRYFMTIPEACSLVLKTGGVGRCGESYLLDMGEPVRIRDLAEQ
                     MIRFYGYEPGRDIRIEYVGLRPGEKVEERLHAADEIIAPTEYPLVNRLERRGPAFPLA
                     EALAALEPACRLSPGLEKDFRNRRRLRAALKKFVPTLMVPEHEPEY"
     gene            3261..4550
                     /locus_tag="PLG14_02130"
     CDS             3261..4550
                     /locus_tag="PLG14_02130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOX47957.1"
                     /translation="MSRNTDFVPFARPSLGVEEESAVLEVLRSGWLTTGKAAKAFEEE
                     FASFVGARRALAVNSATSGLHLALEALGVGEGDLVATSPYTFVSTASVARHLGAEVAF
                     CDIAAEDYGIDPGRLEALLAKEPRIKAVIAIHVGGKPCRMRELLEICGRRGLPLVEDA
                     AHAFPSRVPAALLPEGASRGREAAFAGTLGAIGVYSFYATKTITTGEGGMVVTEDEAL
                     AKRMATMRSHGFDRDAFDRYTSKTASWYYEVAEAGFKYNLPDLLAAIGREQLKKADRF
                     LAEREEIARRYDEAFAPFAADGAASASGQAADRGAAPAGKARPAEALFELPPRGLGHS
                     WHLYSLRLRGPLSGLRNEFFERLQAKGIGASVHFIPLHIMPYFAKRYGLEPGDFPEAF
                     SKYSRTISLPIWQGMDEAQVERTAEAVLETAGELKAR"
     gene            4547..6607
                     /locus_tag="PLG14_02135"
     CDS             4547..6607
                     /locus_tag="PLG14_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOX47958.1"
                     /translation="MTCLSDLAPEGMVHVLTIRSPSARGRIVAVEPPHLPKGYRLAVA
                     ADIPGENRLRSLGVEVPILASGRVAYEGEPLALLAGPDPLVLAELAAQVRVEIEEEEP
                     LLSWETFSSDQVVAKRVAVVGDPELAFSIADKVLEDAYVSGPLEHWYSEPQGALASFD
                     YDKIAVRCATQWPYHVRDSVALALGSRPEEVVVRPTRLGAHLDGKLWYPSLLACHAAL
                     AAFLLKRPARLLLTREEDFRFSPKRARSSVAVRAGLDPTGRLSALDLRIAVNVGAHAP
                     LAEEILSQACLAASGAYACPNIRVEGYAVATNSPPMGAFAGLGAAPSFFALETTAARL
                     AEAAGQDPVEWKARNILRKGSALITGEPLKEEPPYDEIAERLAKASDFRRKWACYELV
                     RKRRTDRGDGPLRGIGFAFAYQGAGSSLSGETPGAYAVEAVLDKELRLLIKTSAAVSG
                     GSAIDIWRNSAAELLGIPLESVAVAPPDTDSAPDSGPVSLSRGIAVVNKLVERSCLAI
                     QKRRFRDPLPISARSVYRFPKPLRWAEGRVEGSPFETAAWGGAVVEVELDAWSLEPRP
                     LGVWLCVDGGRIVSPGRARSALRACAAGALGSCLRESLGCDEGRVAPSAYYRYGLLPL
                     SELPPIEVDFVGAERRSAAKGVGELPFDTVPAAFLAAVSLAAGARLDTLPVGPEILAS
                     TEGA"
     gene            6604..7092
                     /locus_tag="PLG14_02140"
     CDS             6604..7092
                     /locus_tag="PLG14_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX47959.1"
                     /translation="MTVSFILNGEDVAVKARSADRLVDLLRERFGVAGVLPDCRRGVC
                     GKCLVLLDGRLAPSCLLPAFKARGREVVTIEGFAQTDEYGDIKGGFADAGLETCGFCD
                     SGKILAAAALLERNSRPSREEILAEMSAVPCRCTDPEALVKGVQAAADRRARRLYRRA
                     DQ"
     gene            7079..7906
                     /locus_tag="PLG14_02145"
     CDS             7079..7906
                     /locus_tag="PLG14_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOX47960.1"
                     /translation="MLISEVHSPQSLPELLELMRRLPAAALYSGGTELLREQAGKYIF
                     LPPETISIGALPELRQVSLTERYLEIGAAVTLAEILELKESALPELLAGALRGVATPA
                     LRNLATLGGNLATRSRFMDLWAPLACLDALAELKDAAGSRWINVNRLADDEGRPSFPS
                     GALLSRVRVPLERWDAVALRKVGLRDYPAPETAVFALAARAEKGILSDFRLAFAGETA
                     LRLREVEGRILGRRLPLGAKERASFAAEYREAAAGLPSGLRQQFGTLVDGALDLISR"
     gene            7903..>8615
                     /locus_tag="PLG14_02150"
     CDS             7903..>8615
                     /locus_tag="PLG14_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX47961.1"
                     /translation="MIAMSGPKGKLYLVPNVLYPSIARGKAGPAGAAQGDGPSRGEEA
                     AAVDGAGASDGAEASAAAGATAEALLPAAAVARIRSLRRFVVEGEKSAWRLLSSLMDR
                     EALASVSLERLDEHTPASELPGLLKPALDGEDMGLLSDAGVPCVADPGSALAALAHDL
                     GIRVVPLVGPSSLILALSASGLDGQRFSFLGYLPQEPQARRAALASIDRGVRADGATR
                     LFIETPYRNERLLADCLDCL"
     assembly_gap    8616..8715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8820..9599
                     /locus_tag="PLG14_02155"
     CDS             8820..9599
                     /locus_tag="PLG14_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47962.1"
                     /translation="MQKQATLNYEEQYADTVVQWNAFAVQATGLAQGQTLLKVDTYNL
                     ACVPYQFTMRRAVLVGSFSKDEIAFFQRFKNSVAGLSLAFQRPDAREPMKVFCRCQVA
                     GVGMMKDREGVALIVLDWKPIPPDLAEVLGEYLSLVDRLKVEFGDFRDRRVAVTPASA
                     KSLGFNNYAVMSSGGVQTKLALFALAANYLEFLVPMRSADLAPGTEAVFSLYFLSYRF
                     AVQGRIESAERLPTGVQRVRASIGFSAELVHLLEGYFSSRR"
     gene            9672..11036
                     /locus_tag="PLG14_02160"
     CDS             9672..11036
                     /locus_tag="PLG14_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOX47963.1"
                     /translation="MSASASKTRDGFATPIGAFAATLGSAIGLGNIWKFPYLTGSNGG
                     AAFVFAYLASVLLVGLPILVAEHAIGRRSRLDAVRAYRSSVPGSKAWAAIGWAGLASA
                     LLIMAFYTDVAGWVFAYVWKSLRAAAGGPALGPSVFGALVGGTWEPLAWQTAVLALAT
                     AIVAAGVSKGIERATKALMPLLLALLVVCDIRALTLPGAFAGVDYLFKPDLGKLGPGV
                     LLAALGLAFFKLSLGMGTMTTYGSYLPEGTRIVPNAARVALADTLVSILAGLAVFPAV
                     FAFGGEPAGGPGLLFNTIPLVFSKMPGGAVFTVLFFVLASIATVGAMVSIIEVPVAWL
                     TEKGKFGRKAAAAATGAAMLALGVLATLSQGPVLGGVKVFGKNFFDLFDFASSNVLLP
                     LGGLGIAVVAGWLLAKGELGKELGKGYAKLPGSSRAVAALLKFAAPVLILVILLNGLG
                     LFGL"
     gene            complement(11033..14527)
                     /gene="mfd"
                     /locus_tag="PLG14_02165"
     CDS             complement(11033..14527)
                     /gene="mfd"
                     /locus_tag="PLG14_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOX47964.1"
                     /translation="MNTLYPQDVLERLSAHAPLRSLLARLRAGPFPVDISEAEGAFAS
                     VVAAIMARAAPGAGLLVVVPTDQEAESVASDLSLLGLRSDLLPWWRSAAYRAASPRAR
                     AFGDRAAVLARLVLGEARVLVASQRAFVTPLPPRGAFEPLVFALSVGESIDPTALGER
                     LASYGYLRVPRVSLPGEFALRGEVLDLYMPGDEAAVRIVFEYDRIERVASFDPAGQAG
                     LKDLGRVALRPMKELVWSPERTAAVAKAAAAFPNCEGRMGPLLEALAERGEAKGEELW
                     YPVAFGAPAAGGADAGAAPAAGGASAAGGTPASGGAGSILDYLAAGSAVLFLDRERLE
                     SQEEAARKEYAGLYRGALKESPVPPPDRLIHSFVALERRATEAGLRLVRDFALRDKEG
                     ESRLSLGAEPPRSFFGNVTYFKEELASLLKAGYEVRIFAETDSQAERIRTLLKDYAVD
                     VRAAGISAGFAIPALKLLVVQEGEIFGRRKRVPKSVKSARSSVIDTFVELNPGDYVVH
                     INYGIGRFTSIERMRVLGNERDYVKIEYADEENVFVPIEQANLVQRYLGNEGEAPRLD
                     RLGSKSWENRKSKVRKSVEELAERLIRIYARRKAAQGYAFPPDSDWQLAFEASFPYEE
                     TADQLRCIDEVKADMESRKPMDRLVCGDVGYGKTEIAMRACFKAAIAGKQVAFLAPTT
                     ILAEQHFENFKERFEGYPVEIAMMSRLVEKRDQRKTLEGLREGKVDLVIGTHRLLQKD
                     LEWKDLGLIVVDEEQRFGVKDKERLKEMRASVDSLTLTATPIPRTLHMSLLKIRDMSV
                     LQTPPSTRLPIETVVEEFNPELIAEAIRREIARGGQIFYLHNRIESLDEVQTFLGEVV
                     PEVLVETAHGQMDPTELEDIMHRFIHGGFQVLVSTTIIENGIDIPNVNTIVIDRADMY
                     GVSQLYQLRGRVGRSDRPAYAYLFYPDRRALSELAMKRLQIISDFTELGSGFKIAMKD
                     LEVRGAGNLLGREQSGDIYAVGFDLYLKLLDETVARLSDEKWEPEEETYLELEYRGYL
                     PDEYISMPTLKMEVYKKIASIFTDQDLEALRAELLDRFGPLPEEAESLLALAEIRVIC
                     RKLSVASLKERGGVVIVEFSKVAKVSVERVLRLIRESGGRIKLDPHRPNVLVIKTGTI
                     GLKEKSEFLREKLATLLA"
     gene            14621..15655
                     /locus_tag="PLG14_02170"
     CDS             14621..15655
                     /locus_tag="PLG14_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47965.1"
                     /translation="MHRRLPALAAPFLIAAALAALPGSARAAGLAGAPASAASEAGVE
                     LSIRFYDKRIYFPESEIALKVTVTNGSPSTYRFKLAEDRLYSLSFDARTPTNRALDAS
                     DERKLALSASKPVFYREIAVEPGEEYSFVERLERYVRIEGPGSYTVRASFWPELSQRG
                     AAPQGAAAGVQAPGAAPLESNVLILSVRPSPGLPPASEAIRPETGEVMKPEAVPPDEV
                     VRRTIVARQRSRWSEYFLYLDLESLLSRNEDKKRAYDRESDEGRRRMLEKYKADLRES
                     VVDEDISVVPASFEIVETRYAANSGVVRVLAKFDYKSFMMRKEYDYELRRRDDVWYIV
                     GYTVVNKGTE"
     gene            15652..16389
                     /locus_tag="PLG14_02175"
     CDS             15652..16389
                     /locus_tag="PLG14_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX47966.1"
                     /translation="MKALLLIESDVAADIARFYLRPLGFESVRYRDPLKALDNLEEID
                     PDAVILSARDYPRHWKVLAASIRAERSKEDCVLILLKGEFFPFEEASKAIALGVNGVV
                     RDDLDDRREQARFQRLLKRYVSVDEARSSERVAPSDWDRLDFMFSHPQSLVPVAGKLE
                     TISATGLSFAPDSPILASDLGLGQVLEDCSLRAGDRILSPTCEIVRMDRALGLAFRSM
                     DEEEKAYFLKYLGSGPEREMRALLKKG"
     gene            16410..16751
                     /locus_tag="PLG14_02180"
     CDS             16410..16751
                     /locus_tag="PLG14_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOX47967.1"
                     /translation="MQEETRPCPDEYLSEYARKLKAVGHPIRLRLLCLIAREEDPCVT
                     DLWQCLKQPQPVISQHLAILKDNGIVSAEVQKTRRVYSIVDPFIKALIAGIIEAKGTA
                     ALDSLKKGASG"
     gene            16756..>17263
                     /locus_tag="PLG14_02185"
     CDS             16756..>17263
                     /locus_tag="PLG14_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOX47968.1"
                     /translation="MLSLLVEFAVGHRKTRVLMASSPEEAVSPPSALPLLAVADANTA
                     GLVPLPEGRTLVLPPGEASKSWDSLRSALEFASGAGLPRDGTILGLGGGVVCDLAAFA
                     ASLYMRGSRLVLAPTSLVAMADAALGGKAAVDLAGVRNLAGSFYAADEVRLHLPFLLR
                     LPEREYRAG"
BASE COUNT         2425 a         6433 c         5664 g         2641 t
ORIGIN      
        1 cgccgtctcc gccttctcag gaaggggcgg ctcggcgggc ttgaggagta gggcgacgtc
       61 tgccggagcg gcgcttgcgg gagcggcggc cgcgggagcc gccggctgga ccggcgcggc
      121 cgtcttggcc gaagaggagg cgggtgccga acgctcgcgc aaggcgagga gcttcggatt
      181 gaagtcggga tcggaggact cgcccggcct ggcgggaagg gccggagccg ggaccgaggc
      241 cgccggctcg gcgaggacgc gcacgcgggc cgcgtcgccg gccttcatgc cgagttccct
      301 ggcggcgctc ggcgagaggg ccatgaagac gccgggattg ccgaccgtcc ccgtgatggt
      361 gacgacggcc ttcttgccgt tgccgaggtt ctcgacctcg accctcgagt tcttgggaaa
      421 ggagttgcag gagccgtaga ggccctccgc ggggaagtcc cccgcccctc cctggacggc
      481 gctgccctcc cagacggagg aagcgccgac ggcgagcgcg caggccacga gggcggcgac
      541 gatcaggatc cgcttcatca tactcctccg gagccgcctg ggcggccctg cctaagggcg
      601 ttcatcaagg cttcggcggc ctctccgccg agggcgcgga gcagccgcga aaggccctcc
      661 tccgtctcgt ccagtccggc gaagggagga aggctggcct ccgcggccag gcccccgtcc
      721 gcgacccggt agagcctgag ctcgaggccg aggggcctcg ggaggcccgg gcgcagggct
      781 gagggcgcgt aggcgacata gacgcaggcc aggtagtcga cggagccctc gcgggcgtcg
      841 cccagggacc tgggctcgag gcgccaatcg gccctctcga cctgcctggt cctctcgctc
      901 gtcgctatga ggcccgagtc gaagaggcgg tccatgaggc cggagaccac ggcctccgtg
      961 gcctcccccg cgggcggatc gaccgactcg gccgcgagca cggcgacggt ctgggccgag
     1021 gccttgcccg ccatgccgag cgcggccagg acggccgcca gggccagggc ttgcgccctt
     1081 ctcatgctca aaactttcat accctttgtc tatcggctcg cggcgacgcg ccctttacct
     1141 cggagatcgc ccccggcgct cgctcccgga agtcacgcct gttgaatcgg aaggccgccc
     1201 gtgctatcct gccccttcgt ttcccctata gcccctcgcg ggagccctta tatggaatat
     1261 ttcgtgctgc aggtcaagtc gaacgaagag gacgagttca tccgccgggc ttccgtctcg
     1321 cccgccggct gccacggccg cctcttctcc cccaagcgcg tccttccgat ccggcgcggc
     1381 ggcaaggtca cgaacaggga actccccgtc ttccccggct acgtcttcct ggaagtcgac
     1441 tccctggacc gggaggcccg ctgggacttc cgccacgtcg agggcttcta tcgcttcctc
     1501 cgcgacgcgg agggcgatcc cgtccccttg agcgaaaaag accgctccct cctcctgcat
     1561 ttcatatccc tcgggaaacg agccgatata tcgaaggtga ccttcgacga gaaggaccgc
     1621 atcgtcgtga tcgagggagc gctcaagggg ctggagggca ggatagccaa ggtggaccgc
     1681 cgcaagggca gggcccgcgt gatcctggac ctctacgaga agagcttccc catcgatttc
     1741 ggtttcgagg aggtccggaa ggtcggccaa ggaggcgcat cttcccatga cgagtcctga
     1801 gcgagagaac gcgcgcgtct acatcatcgg ggccggcttc gcgggccggg ccatcgcgag
     1861 ggagatcctg gccaagggcg tcataggcca ggtcgtcgcc ttcctggacg acaatcccgc
     1921 caagatcggg ggccgcatcg agggcgtccc cgtcctgggg cccatcagcg acgtcgtgag
     1981 cctcctttcc aggatgcccg ccgacgaggc catcatcgcg atgcccagcg ctccccgaga
     2041 gcgcctccgg gagctctact tcctcttgaa gcgcgccggc ttcgcgcgga tcaggatcgt
     2101 gccgggcgtg gcccagatcc tggagggcga cgcccacctc gtgcaggccc gcgagatcga
     2161 cccccaggac ttcctgggcc gcaatcccgt cgccatcggc ctcaaggaga gcctgtccta
     2221 cctccgcggc aagcgcgtcc tcatcacggg cgcgggcggc tccatcggct ccgagctcgc
     2281 gcgccagctc ctctccgccg gcgtcgcccg cctctacctc ttcggccacg gcgagaactc
     2341 catctaccag atcgaccggg agctcaggct cctccaggcc gagggcgtcg gcgagaggac
     2401 ggccgtcgtc cccgtcatcg gcgagctcaa ggatcccgac tacgtccgct tcgtcctgga
     2461 ccggctcaag gccgacgccg tcttccacgc cgccgcctac aagcacgtcc ccatgatgga
     2521 ggagaacccg atagcggcgg tggagaacaa cgtcttcggc acgaggaacc tcgtggaggc
     2581 ggccctcgcg gccggggtga agcgcctcgt cctcatctcc acggacaagg ccgtggagcc
     2641 ggcttgcgtc tacggcgcct cgaagttcct ctccgagcgc atcgtggagg cggcggcggc
     2701 cagggccgcg cccgggcagt ccttcatggt ggtccgcttc ggcaacgtcc tcgggtcgcg
     2761 cggctccatc gtccccctct tcaagaagca gatcgagcag ggcggccccg tgacggtcac
     2821 gcaccctgag acgagccgct acttcatgac catccccgag gcctgctccc tcgtcctcaa
     2881 gacgggcggc gtgggcaggt gcggagagtc ctacctcctc gacatgggcg agcccgtgcg
     2941 gatccgcgac ctcgcggagc agatgatccg cttctacggc tacgagccgg ggcgggacat
     3001 caggatcgag tacgtgggcc tccggcccgg cgagaaggtc gaggagcggc tccacgccgc
     3061 cgacgagatc atcgccccga ccgagtaccc cctcgtgaac cgcctggagc ggaggggccc
     3121 cgccttcccc ctcgcggagg ccctcgccgc cctggagccg gcctgccgcc tctcgcccgg
     3181 cctcgagaag gacttcagga accgccggag gctccgcgcg gccttgaaaa aattcgtccc
     3241 cacccttatg gtacccgaac atgagccgga atactgattt cgtccccttc gcgcggccct
     3301 ccctcggcgt cgaggaggag tcggccgtcc tcgaggtcct ccgctcgggc tggctcacga
     3361 ctggcaaggc cgcgaaggcc ttcgaggagg agttcgcctc cttcgtcggc gcgaggcggg
     3421 ccctcgcggt caactcggcg acgagcggcc tccacctggc cctcgaggcc ctgggcgtcg
     3481 gcgagggcga cctcgtcgcc acgagcccct acaccttcgt ctccaccgcc tccgtcgcgc
     3541 gccacctggg cgccgaggtc gccttctgcg acatcgcggc ggaggactac ggcatcgatc
     3601 ccgggcgcct cgaggccctc ctcgccaagg agccccggat caaggcggtg atcgcgatcc
     3661 acgtgggcgg caagccctgc aggatgcggg agctcctcga gatctgcgga aggcgcggcc
     3721 tccccctcgt cgaggacgcg gcccacgcct tcccctcgcg cgtccccgcc gccctcctcc
     3781 ccgagggggc gtcccgcggc cgcgaggccg ccttcgcggg caccctgggc gcgatcggcg
     3841 tctattcctt ctacgcgacg aagaccatca cgacgggcga gggcggcatg gtcgtcacgg
     3901 aggacgaggc cctcgcgaag cgcatggcca cgatgcgttc ccacggcttc gaccgcgacg
     3961 ccttcgaccg ctacacctcc aagacggcct cctggtacta cgaggtcgcc gaggcgggct
     4021 tcaagtacaa cctccccgac ctcctggccg cgataggccg cgaacagcta aagaaggccg
     4081 accgcttcct cgccgagagg gaggagatag cccgccgcta cgacgaggcc ttcgccccct
     4141 tcgcggccga cggcgccgcg tccgcgtccg gacaggccgc ggacaggggc gccgcgcccg
     4201 cgggcaaggc gcggcccgcc gaggccctct tcgagctgcc tccccggggc ctcgggcatt
     4261 cctggcacct ctacagcctg cgcctccgcg gccccctttc gggcctccgc aacgagttct
     4321 tcgagcgcct ccaggcgaag ggcatcggcg cctccgtcca cttcatcccc cttcatataa
     4381 tgccctattt cgcgaagcgc tacggcctcg agcccgggga cttccccgag gccttctcca
     4441 agtactcgcg gacgatctcc ctgccgatct ggcagggcat ggacgaagcc caggtcgagc
     4501 ggacggccga ggccgtcctc gagaccgccg gcgagctcaa ggctcgatga cctgcctctc
     4561 cgacctggcc cccgagggca tggtccacgt cctgaccata cgctcgccct cggcgcgagg
     4621 ccggatcgtc gccgtcgagc cgccccatct ccccaagggc taccgcctgg ccgtcgccgc
     4681 cgacatcccc ggcgagaacc gcctgcgctc cctgggcgtc gaggtgccca tcctcgcctc
     4741 gggccgcgtg gcctacgaag gcgagcccct cgccctcctc gcggggcccg accccctggt
     4801 cctggccgag ctcgcggcgc aggtccgcgt ggagatagag gaggaggagc ccctcctctc
     4861 ctgggagacc ttctcctccg accaggtcgt cgccaagcgc gtggccgtgg tcggggatcc
     4921 cgagctcgcc ttctccatcg ccgacaaggt ccttgaggac gcctacgtct ccggccccct
     4981 ggagcactgg tactccgagc cccagggcgc cctggcctcc ttcgactacg acaagatcgc
     5041 cgtgcgttgc gcgacgcaat ggccctacca cgtccgcgac tcggtcgccc tggccctggg
     5101 ctccaggccc gaggaggtcg tggtccgccc gacgcgcctc ggggcccacc tggacgggaa
     5161 gctctggtat ccctccctcc tcgcctgcca cgcggccctc gcggccttcc tcctcaagcg
     5221 accggccagg ctcctcctca cgcgcgagga ggacttccgc ttctcaccca agcgggccag
     5281 gagctccgtc gccgtcaggg cgggcctgga cccgacgggc aggctctccg ccctggacct
     5341 ccgcatcgcc gtcaacgtcg gcgcccacgc gcccctggcg gaggagatcc tctcccaggc
     5401 ctgcctcgcg gcctcggggg cctacgcctg ccccaacatc cgcgtcgagg gctacgcggt
     5461 cgcgacgaac agtccgccca tgggcgcctt cgcgggcctc ggcgcggcac cctccttctt
     5521 cgccctggag accacggccg cccgcctggc cgaggcggcg ggccaggatc ccgtcgaatg
     5581 gaaggcgcgg aacatcctca ggaagggctc ggccctgatc acgggggagc cgctcaagga
     5641 ggagccgccc tacgacgaga tcgcggagcg cctggccaag gcgagcgact tcaggcgcaa
     5701 gtgggcctgc tacgagctcg tccggaagcg caggaccgac aggggcgacg gccccctgcg
     5761 cggcatcggc ttcgccttcg cctaccaggg cgcgggctcc tccctctccg gcgagacgcc
     5821 cggagcctac gccgtggagg cggtcctgga caaggagctc aggctcctca tcaagacgag
     5881 cgccgcggtc tccggcggct ccgccatcga catctggagg aacagcgccg ccgagctcct
     5941 cggcatcccc ctggagagcg tcgcggtcgc gccgcccgac accgactcgg cccccgactc
     6001 cgggcccgtg agcctctccc gcggcatcgc cgtcgtcaac aagctcgtcg agcggagctg
     6061 cctcgccatc cagaagcggc gcttccgcga tcccctgccc atctccgcga ggagcgtcta
     6121 ccgcttcccc aagcccctgc gctgggccga gggccgcgtc gagggctcgc ccttcgagac
     6181 ggccgcctgg ggcggagccg tcgtggaggt cgagctcgac gcctggagcc tggagccgag
     6241 gcctctcggg gtctggctct gcgtcgacgg cgggcgcatc gtctcgcccg gccgcgcccg
     6301 ctccgccctc cgcgcctgcg cggcgggcgc cctcggctcc tgcctccgcg agagcctcgg
     6361 ctgcgacgag gggcgcgtcg cgccctccgc ctactaccgc tacggcctcc tccccctctc
     6421 cgagcttccg cccatcgagg tggacttcgt cggggcggag cggcgctcgg ccgcgaaggg
     6481 cgtgggcgag cttcccttcg acaccgtccc cgcggccttc ctcgccgccg tctcgctggc
     6541 cgcgggcgcc cgcctcgaca ccctgcccgt cgggcccgag atcctcgcct ccacggaggg
     6601 agcatgaccg tcagcttcat cctcaacggc gaggacgtcg ccgtgaaggc ccgctccgcc
     6661 gaccgcctcg tcgacctcct ccgcgagcgc ttcggcgtcg ctggcgtcct gcccgactgc
     6721 aggcgcggcg tctgcggcaa gtgcctcgtc ctcctggacg gaaggctcgc cccctcctgc
     6781 ctcctccccg ccttcaaggc gcggggccgc gaggtcgtca ccatcgaggg cttcgcccag
     6841 accgacgagt acggggacat caaggggggc ttcgccgacg cgggcctgga gacctgcggc
     6901 ttctgcgact ctggcaagat cctcgcggcc gccgccctcc tggagcggaa ctcccgcccc
     6961 tcgcgggagg agatcctggc cgagatgtcc gccgtgccct gccgctgcac cgaccccgag
     7021 gccctggtga agggcgtgca ggccgccgcc gaccgccgcg cgaggaggct gtaccgccgt
     7081 gctgatcagt gaggtccaca gcccccagag cctgcctgag ctcctcgagc tcatgcgccg
     7141 cctgcccgcc gccgcgctct actcgggcgg caccgagctc ctccgcgagc aggcggggaa
     7201 gtacatcttc ctgccgcccg agaccatctc catcggcgcc ctgcccgagc tcaggcaggt
     7261 gtccctcacg gagcgctacc tcgagatagg cgcggccgtc accctcgccg agatcctcga
     7321 gctcaaggag agcgccctgc ctgagctcct ggccggagcc ctgcgcggcg tcgcgacgcc
     7381 ggccttgcgc aatctcgcga ccctgggagg caacctcgcg acgaggagcc gcttcatgga
     7441 cctgtgggcc cccctcgcct gcctggacgc cctggccgag ctcaaggacg ccgcgggctc
     7501 gcgctggatc aacgtgaacc ggctcgccga cgacgaggga aggccatcct tcccctccgg
     7561 cgccctcctc tcgcgcgtcc gcgtccccct ggagcgctgg gacgccgtcg ccctgcggaa
     7621 ggtgggcctc cgcgactatc ccgcgccgga gaccgccgtc ttcgccctgg cggcccgcgc
     7681 ggagaagggc atcctctccg acttccgcct ggccttcgcc ggcgagaccg ccctccgcct
     7741 ccgcgaggtc gagggccgca tcctgggccg gcgccttccc ctcggcgcga aggagcgcgc
     7801 ctccttcgcc gccgagtacc gcgaggccgc cgcgggcctg ccctcgggcc ttcgccagca
     7861 attcgggacc ctcgtggacg gggccctcga tctcatctcg aggtgattgc gatgtccggc
     7921 cccaagggaa agctctatct cgtgcccaac gtcctctacc cctccatcgc gaggggcaag
     7981 gccggccccg cgggcgcggc gcagggggac ggaccttcgc gcggcgagga agcggcggcc
     8041 gtcgacggcg ccggcgcttc cgacggcgcg gaagcctccg ctgccgccgg cgcgaccgcg
     8101 gaggccctcc tccccgcggc cgcggtcgcg cgcatacgat ccttgcgccg cttcgtcgtc
     8161 gagggggaga agtccgcgtg gcgcctcctc tcctcgctca tggaccgcga ggccctcgcc
     8221 tccgtcagcc tggagcgcct ggacgagcac acgcccgcct ccgagctccc cggcctcctc
     8281 aagcccgccc tggacggcga ggacatgggc ctgctctccg acgccggcgt gccctgcgtc
     8341 gccgaccccg gcagcgccct cgccgccctg gcccacgacc tgggcatcag ggtcgtcccc
     8401 ctcgtcggcc cctcctccct catcctcgcc ctctcggcct cgggcctgga cggccagcgc
     8461 ttcagcttcc taggctacct gccgcaggag ccccaggccc gccgcgccgc cctcgcctcc
     8521 atcgaccggg gcgtccgcgc cgacggcgcg acgcggctct tcatcgagac gccctaccgc
     8581 aacgagcgcc tcctggccga ctgcctggac tgcctnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnngccga gcccctcgtc ggcaaggagc cggccgtctt cctggtcggc
     8761 agggcgccgg gaacgagcct tgaccgcccg tccggcttcg atcatagttc cacgcggata
     8821 tgcaaaaaca agcgacgctg aactacgagg agcagtacgc ggataccgtc gtccaatgga
     8881 acgccttcgc ggtccaggcg acgggcctcg cgcagggcca gaccctcctc aaggtcgaca
     8941 cctacaacct ggcctgcgtg ccctaccagt tcacgatgcg ccgcgccgtc ctcgtcggct
     9001 ccttctccaa ggacgagatc gccttcttcc agcgcttcaa gaactcggtc gcgggcctga
     9061 gcctcgcctt ccagcgcccc gacgcccgcg agccgatgaa ggtcttctgc cgctgccagg
     9121 tagcgggagt gggcatgatg aaggaccgcg agggcgtcgc cctcattgtc ctggactgga
     9181 agcccatccc tcccgacctg gccgaggtgc tcggcgagta cctctcgctc gtcgaccggc
     9241 tcaaggtcga gttcggcgac ttccgcgacc gccgcgtcgc cgtcaccccg gccagcgcga
     9301 agagcctcgg cttcaacaac tacgcggtga tgagctcggg cggagtccag accaagctcg
     9361 ccctcttcgc gctggccgcc aactacctcg agttcctggt ccccatgcgc tcggccgacc
     9421 tggccccggg cacggaggcc gtcttcagcc tctacttcct ctcctaccgc ttcgccgtgc
     9481 aggggcggat cgagtcggcg gagcgcctgc cgacgggggt ccagcgcgtc cgcgcctcca
     9541 tcggcttctc cgccgagctc gtccatctcc tggaaggcta cttcagctcg aggagatgag
     9601 cgccgtttcc acctgattgc gcttgacagg ccttcgatgt ttctttatac agatacgtca
     9661 tcggaggcac catgtccgct tcagcatcca agacccgcga cggcttcgcc actcccatcg
     9721 gggctttcgc cgccacccta ggctcggcca tcgggctcgg caacatctgg aaattcccct
     9781 acctcacggg ctcgaacggc ggcgctgcct tcgtgttcgc ctacctcgcg agcgtcctcc
     9841 tcgtgggcct gcccatcctc gtcgccgagc acgcgatagg gcgcaggtcc aggctcgacg
     9901 cggtgcgggc ctaccgctcc tccgtccccg gctccaaggc ctgggcggcc ataggctggg
     9961 ccggcctcgc ctccgccctc ctcatcatgg ccttctacac cgacgtcgcc ggctgggtct
    10021 tcgcctacgt ctggaagtcc ttgcgcgccg cggcgggcgg ccccgccctg ggaccctccg
    10081 tcttcggagc cctggtgggc ggcacctggg agcccctggc ctggcagacc gccgtcctcg
    10141 ccctcgcgac ggccatcgtc gccgccggcg tctccaaggg catcgagcgg gctaccaagg
    10201 ccctcatgcc cctcctcctc gccctactcg tcgtctgcga catccgcgcc ctcactctcc
    10261 ccggggcctt cgccggcgtc gactacctct tcaagcccga cctcgggaag ctcgggcccg
    10321 gcgtccttct cgcggccctg ggcctggcct tcttcaagct ttccctaggc atggggacga
    10381 tgaccaccta cggctcctac ctccccgagg ggacgcgcat cgtgccgaac gcggcccgcg
    10441 tcgccctagc cgacaccctc gtgtccatcc tcgccggcct ggccgtcttc cccgcggtct
    10501 tcgccttcgg gggcgaaccc gcgggcggcc ccggcctcct cttcaacacg atccccctcg
    10561 tcttctccaa gatgccggga ggggcggtct tcaccgtcct cttcttcgtc ctggcctcca
    10621 tcgccaccgt gggcgcgatg gtgagcatca tcgaggtgcc cgtggcctgg cttacggaga
    10681 agggcaagtt cgggcggaag gccgccgccg ccgccacggg agccgcgatg ctcgccctcg
    10741 gcgtcctggc gacgctctcg caggggcccg tcctgggcgg agtgaaggtc ttcgggaaga
    10801 atttcttcga cctcttcgac ttcgcctcgt ccaacgtcct cctgcccctg ggaggcctcg
    10861 gcatagcggt cgtggcgggt tggctcctcg cgaagggaga gctcgggaag gagctcggca
    10921 agggttacgc gaaactgccc ggctcctctc gggccgtggc cgccctcctc aagttcgcgg
    10981 cccccgtcct gatcctcgtc atcctcctaa acggcctcgg gctcttcggg ctctaggcga
    11041 ggagggtcgc gagcttctcg cggaggaact cgctcttctc cttgaggccg atcgtccccg
    11101 tcttgatgac gaggacgttc ggcctgtggg gatcgagctt gatccgcccg cccgactccc
    11161 tgatgagccg caagacccgc tccacggaga ccttcgcgac cttggagaac tcgacgatga
    11221 cgacgccgcc gcgctccttg aggctcgcga cggagagctt ccggcagatg acgcggatct
    11281 ccgcgagggc caggaggctc tccgcctcct cgggcagggg gccgaagcgg tccaggagct
    11341 cggcccgcag ggcctcgagg tcctggtccg tgaagatgga ggcgatcttc ttgtagacct
    11401 ccatcttgag ggtcggcatc gagatgtact cgtcggggag gtagccccgg tactctagct
    11461 cgaggtaggt ctcctcctcg ggctcccact tctcgtcgga gaggcgggcg acggtctcgt
    11521 cgaggagctt gaggtagagg tcgaagccga ccgcgtagat gtcgcccgac tgctcgcgcc
    11581 cgaggaggtt gccggccccg cgcacctcca ggtccttcat cgcgatcttg aagcccgagc
    11641 cgagctcggt gaagtcggag atgatctgga gccgcttcat cgcgagctcg gagagggcgc
    11701 ggcggtcggg gtagaagagg taggcgtagg ccggccggtc cgagcggccc acgcggccgc
    11761 ggagctggta gagctgggag acgccgtaca tgtccgcccg gtcgatgacg atcgtgttga
    11821 cgttggggat gtcgatgccg ttctcgatga tggtcgtcga gacgaggacc tggaagccgc
    11881 cgtggatgaa gcggtgcatg atgtcctcga gctcggtcgg gtccatctgg ccgtgcgcgg
    11941 tctccacgag gacctccggc acgacctcgc ccaggaaggt ctggacctcg tccaggctct
    12001 cgatgcggtt gtggaggtag aagatctggc cgccgcgcgc tatctcgcgc ctgatggcct
    12061 cggcgatcag ctcggggttg aactcctcga cgaccgtctc tatcggcagc cgcgtcgagg
    12121 gcggggtctg gagcaccgac atgtcccgga tcttgaggag ggacatgtgg agggtgcgcg
    12181 gtatgggcgt cgccgtaagg gtcagggagt cgacgctcgc gcgcatctcc ttgagccgct
    12241 ccttgtcctt gacgccgaag cgctgctcct cgtccacgac gatgagcccc aggtccttcc
    12301 actcaaggtc cttctggagg aggcggtgcg tgccgatgac gaggtcgacc ttcccctcgc
    12361 gcaggccctc cagggtcttc ctctggtcgc gcttctccac gaggcgcgac atcatcgcga
    12421 tctccacggg atagccctcg aagcgctcct tgaagttctc gaagtgctgc tccgcgagga
    12481 tcgtcgtcgg ggccaggaag gcgacctgct tgcccgcgat ggcggccttg aagcaggcgc
    12541 gcatcgcgat ctccgtcttg ccgtagccga cgtcgccgca gaccaggcgg tccatcggct
    12601 tccggctctc catgtcggcc ttcacctcgt cgatgcagcg cagctggtcg gcggtctcct
    12661 cgtaggggaa ggaggcctcg aaggcgagct gccagtccga gtcggggggg aaggcatagc
    12721 cttgcgcggc cttgcgcctc gcgtagatgc ggatgaggcg ctcggccagc tcctccaccg
    12781 acttccggac cttgctcttc ctgttctccc aggacttgga gcccaggcgg tccaggcggg
    12841 gagcctcgcc ctcgttgccg aggtagcgct ggacgaggtt ggcctgctcg atcgggacga
    12901 agacgttctc ctcgtcggcg tactcgatct tcacgtagtc gcgctcgttg cccaggacgc
    12961 gcatccgctc gatggaggtg aagcggccga tgccgtagtt gatgtggacc acgtagtcgc
    13021 ccgggttgag ctccacgaag gtgtcgatga ccgaggacct ggcgctcttc accgacttcg
    13081 gcacgcgctt gcggcggccg aagatctccc cctcctggac gacgaggagc ttgagcgcgg
    13141 ggatggcgaa gcccgcggag atgccggcgg cgcgcacgtc caccgcgtag tccttgagga
    13201 gggtccgtat gcgctcggcc tgggagtccg tctccgcgaa gatcctcacc tcgtagccgg
    13261 ccttgaggag ggaggcgagc tcctccttga agtaggtcac gttgccgaag aaggaccgcg
    13321 gcggctccgc gccgaggctc aggcgcgatt cgccctcctt gtcgcgcagg gcgaagtccc
    13381 gcacgagcct gaggcccgcc tccgtcgccc gccgctccag ggcgacgaag gagtggatga
    13441 ggcggtcggg gggcgggacc gggctttcct tcagggctcc tcggtagagg ccggcgtact
    13501 ccttgcgggc cgcctcctcc tgggactcga ggcgctcccg atccaggaag agcacggcgc
    13561 tgcccgcggc gaggtagtcg aggatggacc ccgccccgcc cgaggcggga gttccgccag
    13621 cggcggaggc cccgccagcg gcgggcgcag cccccgcgtc ggcgccgcca gccgcggggg
    13681 cgccgaaggc gaccgggtac cagagctcct cgcccttggc ctcgccccgt tccgccaggg
    13741 cctccaggag ggggcccatg cggccctcgc agttcgggaa ggccgcggcg gccttggcca
    13801 cggcggccgt cctctcggga ctccagacga gctccttcat gggccgcagg gcgacccgcc
    13861 ccaggtcctt gaggccggcc tggcccgcgg gatcgaagga ggcgacgcgc tcgatgcggt
    13921 cgtactcgaa gacgatacgg acggcggcct cgtcgccggg catgtagagg tccaggacct
    13981 cgccgcgcag ggcgaactcg cccggcaggg agacgcgggg gacgcggagg tagccgtagg
    14041 aggcgagtcg ctcgcccagg gccgtcgggt cgatgctctc gccgacggag agggcgaaga
    14101 ccaggggctc gaaggccccg cggggcggga ggggcgtgac gaaggcgcgc tggctcgcga
    14161 cgaggacgcg ggcctcgccc aggacgagcc tcgcgaggac ggcagcgcgg tcgccgaagg
    14221 cgcgggcccg cggggaggcg gcgcgatagg cggcgctgcg ccaccagggc aggaggtcgg
    14281 agcggaggcc gaggagggag aggtcgctcg ccacggactc cgcctcctgg tcggtcggga
    14341 cgacgacgag gaggcccgcg ccgggcgccg cgcgggccat gatggccgcg acgacggagg
    14401 cgaaggcgcc ttccgcctcg gaaatgtcga cgggaaaggg gcccgcgcgg aggcgggcta
    14461 ggaggctcct caggggagcg tgggcggaca ggcgctcgag gacgtcttgc gggtataagg
    14521 tattcatcgt aacattccga caataaaggg tggaaagccg ttcgcggggt ataatacacc
    14581 caggcccggc ctttgttcca tatcgaagga gagaacggtc gtgcatcgca gattgcccgc
    14641 cctcgcggcg cccttcctca tcgcggccgc cctcgcggcc ctgccgggct ccgcccgcgc
    14701 ggccggcctt gccggcgcgc ccgcctccgc ggcctcggag gccggcgtcg agctctccat
    14761 ccgcttctac gacaagcgga tctacttccc ggagagcgag atagccctca aggtgacggt
    14821 gacgaacggc tcaccgtcga cctaccgctt caagctcgcg gaggaccgcc tctacagcct
    14881 ctccttcgac gcgcgtacgc cgaccaaccg ggccctcgac gcctccgacg agcgcaagct
    14941 cgccctctcg gcctcgaagc ccgtcttcta ccgcgagatc gcggtggagc ccggcgagga
    15001 gtattccttc gtcgagcgcc tggagcgcta cgtccggatc gagggccccg gctcctacac
    15061 ggtccgcgcc agcttctggc ccgagctctc ccagcggggc gccgcccccc agggcgcggc
    15121 ggcgggagtc caggctccgg gcgcggcgcc cctcgagtcg aacgtcctca tcctctcggt
    15181 gcggccctct ccgggcctcc ctcccgcctc cgaggccatc cgccccgaga cgggcgaggt
    15241 catgaagccc gaggccgtcc cgcccgacga ggtcgtgcgc cgcaccatcg tcgccaggca
    15301 gcgctcccgc tggagcgagt atttcctcta cctcgacctc gagtccctgc tctcccgcaa
    15361 cgaggacaag aagcgcgcct acgaccggga gtccgacgag ggccggcgcc gcatgctgga
    15421 gaagtacaag gccgacctcc gggagagcgt cgtggacgag gacatctccg tcgtgcccgc
    15481 gagcttcgag atcgtggaga cgaggtacgc cgccaactcg ggcgtggtca gggtgctcgc
    15541 gaagttcgac tacaagagct tcatgatgcg caaggagtac gactacgagc tcaggcgccg
    15601 cgacgacgtc tggtacatcg tcggctacac ggtcgtcaac aagggcaccg aatgaaggcc
    15661 ctcctcctca tcgagagcga cgtcgccgcc gacatcgcgc gcttctacct ccggcccctc
    15721 ggcttcgagt cggtgcgcta ccgcgacccc ctcaaggccc tggacaacct ggaggagatc
    15781 gaccccgacg ccgtcatcct ctccgcccgg gactatcccc gccactggaa ggtcctggcc
    15841 gcctccatcc gcgccgagag gtccaaggag gactgcgtcc tcatcctcct caagggcgag
    15901 ttcttcccct tcgaggaggc ctccaaggcc atagccctgg gcgtgaacgg cgtcgtcagg
    15961 gacgacctgg acgaccgccg cgagcaggcc cgcttccagc gcctcctcaa gcgctacgtg
    16021 agcgtggacg aggcccggag ctcggagcgc gtggctccct cagactggga ccgcctcgac
    16081 ttcatgttct cccaccccca gagcctcgtc cccgtggccg gcaagctgga gacgatctcc
    16141 gccacgggcc tctccttcgc gccggactcc cccatcctcg cctctgacct ggggctgggc
    16201 caggtcctgg aggactgctc gctgcgcgcc ggggaccgca tcctctcccc cacctgcgag
    16261 atcgtgagga tggaccgggc cctgggcctg gccttccgga gcatggacga ggaggagaag
    16321 gcctacttcc tcaagtacct tggctccggc cccgagcgcg agatgcgcgc cctgttgaaa
    16381 aaaggctgaa atagctatac tatgctcgaa tgcaggaaga aacgcgcccc tgtccagacg
    16441 agtacctctc ggaatacgcg cgaaagctga aagccgtagg ccatcccatc aggctcaggc
    16501 tcctctgcct catcgcgcgc gaggaagatc cctgcgtcac cgacctctgg cagtgcctca
    16561 agcagcccca gcccgtcatc tcgcagcacc tcgcgatcct caaggacaac ggcatagtct
    16621 cagccgaggt ccagaagacc cgccgggtct actccatcgt cgatcccttc atcaaggccc
    16681 tcatagccgg catcatcgag gccaagggca cggccgccct ggacagcctg aagaaaggcg
    16741 cctcgggcta ggcccttgct ctccctcctc gtcgaattcg ccgtaggaca ccgcaagacc
    16801 cgggtgctca tggcctcgag ccccgaggag gccgtctccc ccccctcagc cctccccctc
    16861 ctagccgtcg ccgacgccaa caccgccggc ctggtccccc tgcccgaagg taggaccctc
    16921 gtcctccccc ccggcgaggc ctccaagagc tgggacagcc tccgctcggc cctcgaattc
    16981 gcctcaggcg cgggcctccc gcgggacgga accatcctgg gcctgggcgg cggcgtcgtc
    17041 tgcgacctgg cggccttcgc cgcctccctc tacatgcgcg gctcccgcct cgtcctcgcg
    17101 ccgacgagcc tcgtcgccat ggccgacgcg gccctgggcg gcaaggcggc ggtcgacctg
    17161 gcgggcgtgc gcaacctcgc aggctccttc tacgcggcgg acgaggtgcg gctccacctg
    17221 cccttcctcc tccgcctccc cgagcgcgag taccgcgccg gcc
//
LOCUS       DAOOSJ010000021        16702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2375_length_16702_cov_6.061693, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000021 DAOOSJ010000000
VERSION     DAOOSJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 16702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16702
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_16702_cov_6.061693"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..760)
                     /gene="pyrH"
                     /locus_tag="PLG14_02190"
     CDS             complement(62..760)
                     /gene="pyrH"
                     /locus_tag="PLG14_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOX47969.1"
                     /translation="MTTVLSLGGSIVAPEGPDPAFLKAFRALVEAHLAVDPARRLILV
                     VGGGGPARLWQKAFREAARPEEAADAAAQDWIGVMATRLNAELVRALMGPLSPQDVVT
                     DPTAVTIFMGRVLVAAGWKPGWSTDYDAVLLAEKFAAKEVVNLSNIEKVYTADPRKDP
                     SAKPIDAISWEGFRKLVGDEWKPGANAPFDPIATRRASEIGLRVVCAAGRNLENLAAL
                     LEGKPFVGTTIGPD"
     gene            complement(915..2048)
                     /locus_tag="PLG14_02195"
     CDS             complement(915..2048)
                     /locus_tag="PLG14_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOX47970.1"
                     /translation="MNVKRLLLALILASSVPALWAAGATPVGRIDYVEGGVTITRAGK
                     SLGDPNPDDPLLSGDLVKTSSDGLLVIAMDKTTGMGGTITVKAKSALYLKLDESKGEK
                     RTQIDLLAGSVASKVKKLSGSPTMQVTTGGTVAGVRGTEFEVAISVNEAALVVCSEGE
                     VSFGDGKEEFPVPAGKAAEKRPGERLRYLPVAVSSVRQFREKWVADEVAAFRADPARA
                     LASYEKRYAELSSSFNQAFDPFQKSEVLKKWMDEDRAGVKPRGLDPAVMREKKAIVGD
                     LQKLRKILVIFERVYYRLDEIDGLVAGTSLERRELRPGLTAGDFLRKVRDERDTLSRR
                     VALFRYAELLYSFRNEGGTGMPGSSGGDDFFGSDEDFFGSSDF"
     gene            complement(2228..3520)
                     /locus_tag="PLG14_02200"
     CDS             complement(2228..3520)
                     /locus_tag="PLG14_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47971.1"
                     /translation="MKLKTAVTTAAALLLGSALAFAQSGTEPSESYVLDKAREAYQGS
                     QAEQPQAAPAPEAPPAPYYPTAGESGESDSVPRYPFVFTALPGLSLPFGEFDVDVQLG
                     LIMATARDVDGLQAASVFANARDVEGFQAAGVFANARRLSGFQASGVFNTASEDVEGF
                     QGSGVFNTAAGDLVGFQGSGVFNTVAGDVEGYQGAGVFNVAGGRVSGAQMAGVANLAA
                     GDVEGLQMAGVYNQARRVEGLQIGLVNVADDIEDGLQLGLVNIARNGVSGVVGYWEPD
                     SDELGFAWQNGSRSLFTVLRVSAPRGDWLETSDSAIAAFGLGTRMGGRRGSPYLDLEA
                     SAAREIGPLLDHLADCAAREEWTEPSFSQRPYPSFRLSLGVPLAGRLHLLGGLNVDVD
                     LADWPALPERRKAGSSFSDTWFGCSFTAYSRWFLGFRI"
     gene            3793..>4288
                     /locus_tag="PLG14_02205"
     CDS             3793..>4288
                     /locus_tag="PLG14_02205"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOX47972.1"
                     /translation="MGFLSAWRERRRERAALLHPVPDELWDWAVKEHRIFRWLCPADL
                     ARLRSLAAAFLAEKTFDPVGGAVVDEELRVSVAAQACLPLLGLPGGLDWYRDFSTIIV
                     TPREYAVRKRDWDEAGVVHEYDDEFAGEAFDLGPVALSRVDIEASGWGDGYNVVIHEM
                     AHKLD"
     assembly_gap    4289..4388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4473..5534)
                     /locus_tag="PLG14_02210"
     CDS             complement(4473..5534)
                     /locus_tag="PLG14_02210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOX47973.1"
                     /translation="MNDPEREPDIDDLIRLSVFSSIGTSIVAENSIRGVLDRVMEQVG
                     SFFGPLNWSVLLVDAERDELVFSVVVGRAADDLRGKRVPLDEGIAGWVARRGLPAFVE
                     DVAGDSRFAPRADQISGFETKSVIAVPLRTGSKVFGVIELLNRLDGGSFSAFDLRVLS
                     TIADFAAIAIEKCYYLAEARRLSEIDSLTGVLNRRGLAKVIERETARVKRYAGNLSVV
                     VGDVDAFKRINDENGHAAGDEVLKAVAAVFRKEAREADSVARFGGDEFIVVMPATGPE
                     DAEIARARIQEALDKAGAAHPVPFSVTLGVHSANEADFDELFRESDRDLYRRKTAGPL
                     ALGENLLAALDEEARRREP"
     gene            5780..6979
                     /locus_tag="PLG14_02215"
     CDS             5780..6979
                     /locus_tag="PLG14_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX47974.1"
                     /translation="MASYEFSETAGRIVRSEIREMLKWSRRPDTVSFGGGLPDPSLFP
                     VEELEAISARVLGKKGYLALQYGPTPGEPAAVEAFVRHMADFGDEALPANLCVTSSSQ
                     QALDLLSLLFVDPGSPVVVEMPSYVGALQAFGRAGADFRGVPVGRDGMDLDRLEAELR
                     RLDAEGRKPRFVYVIPDFQNPAGSTMGLEARRRLLSIARRRGLLLVEDSPYRELAFDG
                     RALPSLWSLAEGKGVVLLKTLSKMLFPGFRLGWICAEEALAERFECMKQSVDLCTSSF
                     VQLLAAEYIGEGRMRGTIERARALYRPKRDAMLAGLDAVLPPGSRRSEPEGGVFLWVE
                     LPEGWDAARVLRAALAEGVAFVTGGSFFHEGSGRNCMRLNYSFPSAKAIGRGCERLGR
                     ALAAARP"
     gene            7052..>8523
                     /locus_tag="PLG14_02220"
     CDS             7052..>8523
                     /locus_tag="PLG14_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX47975.1"
                     /translation="MVKGVESGALRAGDALPSTRRLAEELGVSRFTAERAYEELWAEG
                     YVEARSGAATRVRARPPRASGAAPGEAAGRRAGGGSRPAAEGERVTGGARGRTSGEAR
                     LETLVDAALEEALGAAAGLRSIHAAPAAGRPGAVDFARFNLDPRLYPVAAFRRSLAAA
                     LAEDPARALSYGPPEGDPALRALIARRMAEHGAACSPERIVLVDGALHGLDLVLRLLV
                     PRGGSVLVEAPTFSGAILLARASGLEVVGAETDGEGLLPAAAEEAYERASAAGRRPSL
                     LYVTPSFHNPTGSLAGQGRREEILAFAASRGLPLVEDCFEEEIGGPGARAKPIAAMDG
                     EGRTIYLGTFSKVLFPGVRLGWIAAAPALARRLALMRQATGVSGNSLVQAAVAGFMSS
                     GAYGAHVRRVNRVFSRRLAAAVSAFRRFVPEGAARLFEPAGGYLLWLELPAGRGGAAR
                     AETARARVARAEAEAVEACAAEGLLVAPGASFWPGEPPGA"
     assembly_gap    8524..8623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8661..9785)
                     /locus_tag="PLG14_02225"
     CDS             complement(8661..9785)
                     /locus_tag="PLG14_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX47976.1"
                     /translation="MDEILLEAFGCFVLECDELVQKAVDSTLELEDGGGAEAVDALMR
                     ALHTIKGNARMFELEAIPAIAHAAESLAARLKREGLRSGRAETNLLLESLDAIKSRVA
                     AVAAGSKEEAARPELLAALESARAPAPAASGLAATTAAPRAASRPTPAAQSAAAQAAP
                     ASQPAAAPAAPALRPAVAAHPTAAPRAEARPAAAPPAVDKPAPFEARAEPSSAAAAPS
                     VRPSAAAAGSRALSILLVDDDFLQRRSSQHILAQYGSCAVAAGGDEAVEAVARSLEPG
                     AEPFDLIVVDLVMPGMDGFEVVRSIRSLEMAFALEELSSGAAGVGDLARKDARILVAS
                     VLDDPASYMKACFRCGADGYLLKPVEPARLEKELARLGLA"
     gene            complement(9871..10533)
                     /locus_tag="PLG14_02230"
     CDS             complement(9871..10533)
                     /locus_tag="PLG14_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOX47977.1"
                     /translation="MASICEFTTPNERRYKPAEEIANAATHGLGALLSIAALVLLVRA
                     AAAGSDGLGVLGYALFGASLILLFGASATYHGVASPRAKKRLEVLDHASIYALIAGTY
                     SSFCLTVLRGPLGRTLFAVVWALAVAGAALKVAFINKFRLLSTLGYVAMGWLIVFAFA
                     PLRAALPPASLNLLFAGGVAYTVGAAFYALKKVPWFHPLWHLFVLAGAACHVLSALLS
                     MG"
     gene            10635..12506
                     /locus_tag="PLG14_02235"
     CDS             10635..12506
                     /locus_tag="PLG14_02235"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX47978.1"
                     /translation="MNILSALSLLACAFYLESGLRVLFLDRKGRINRLSFALYLDLCL
                     WAFAAAFIYGASLPETSRAWHLAFSPTWNLFFGFALHFCLLAAGRDFRLTPWRAFLLY
                     APGLFFTLYSNLFRLESFLLHGGYWMPVFRPGLSYWLFVAYYSVYLGFGLLALAQAFL
                     RSKDELERRRLGIFLASTSLPFVLGFLSDSVAPALGLDLPNLGILWIGIWAFGTRVAM
                     TRYGFFAPVHEEIERRVRAEARLADAGRLFDAFLENSLDGIVLTDAEGRVSRWSAAME
                     RISGLSADAVVGRPIWEIQASMAWAGDKEELSRSIEAEMRKLLAGKEAGWLDEVSEYR
                     VRDGKGEEKYLQASSFVIPMSGGRQAAAAIVRDATERKRAETEALEELRRLDNAQKME
                     AVGTLAAGLAHDFNNILTGVLGTVSLLRLEAGEGGRVEPEELSGGLGIIETQASRAVE
                     ISRQLLDLTKRHPAEERPTSLGAAIQKAAALTRGGLDPAVELLVETPPPEAYVLSEGS
                     ELERVLINLILNSGEAMTSMRGPGEAPGGAVRVSAFREGSFWAISVKDEGVGIAREAL
                     ARVFDPFYTTKGGGKSMGLGLSIVYNIAKRRGGRVEVDSAPGEGAEFRVYLPACETP"
     gene            complement(12523..13287)
                     /locus_tag="PLG14_02240"
     CDS             complement(12523..13287)
                     /locus_tag="PLG14_02240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX47979.1"
                     /translation="MIEAGALGKKVVFLYPPPVLTKVVEELSRREFEVYLVRDHARLR
                     RVLAAQPDALVFVNIDAELEEEAWEDYVKSLRSDPKTAGVGVGVLTLNEDKDLSSKYL
                     MDIGVPCGFIVLKIGAAKTIDILAKTLEANEARGRRKFVRALCPPGSAQVTVELDGET
                     VRGEASDISAAGCAIAFEGERSYKPGTILRGLQLTVKGVRLLVDGFVAARHDGARAAH
                     VIMFAPASLDEGKREKLRSLIFRMNQAAMDRLIESN"
     gene            complement(13420..13887)
                     /gene="msrB"
                     /locus_tag="PLG14_02245"
     CDS             complement(13420..13887)
                     /gene="msrB"
                     /locus_tag="PLG14_02245"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOX47980.1"
                     /translation="MPIVKAADAANVCYVQPTEELKARLGPERFAILAQGATEPPFQN
                     AYWDNHEEGVYLDAIDGTPLFASREKYESGSGWPSFWAPIDQSRLLLVEDLSYGMRRI
                     EVKAKASGGHLGHLFEDGPEPTGLRYCINSASLRFVPRAGLAAEGLGELESLF"
     gene            complement(14298..16598)
                     /locus_tag="PLG14_02250"
     CDS             complement(14298..16598)
                     /locus_tag="PLG14_02250"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOX47981.1"
                     /translation="MELDLSRPWRLVLADPSSPSGIAAPEDLARLLANPESAADPARF
                     AAAELASSLGRIASSPIVAGEGSEAEGLILLDAGPEGLPRGGAAGEHGGAGGASGAAG
                     ADGGAGGQAAPDAAAFAAPARRGFAWRASPTRVELHGDSPEGLLACVYDFLEAGGFRW
                     IEPGPEGERLPAPGSLRLERASGRSDRPALRPVLILGHGAYLEKAADYLLFAARNGYA
                     GVFLHTIEGPLALGACPAARLDGGRASLAALAGRLGLELEEGGHLLSSLLPRSLSKRE
                     PELFRMTRGGRKADRNFCPSNPRSLALAAESFARFAKARPEVRVFHCWPDDLPGGGWC
                     SCPACVDAEGGPLSAAAQSLKAALALASALAEARPGARLSFLAYHDTEELAGALKALG
                     LGEASLPGNLELLWAPRRRSWASGYGESGSALNAASRGAFETALRDFAAAPGGASVFE
                     YYEDALLFKGAVPPLGTTMAADLAWYSGESPDSSGARASKVGILLTGGRLPLAPRPNL
                     WLFPRLAALGPGGPAALEALLADWAGAAYGQAAAPMLRYWKALEAAWAIDLDLEPGET
                     ENRIPAPLRNAVQEPPADWGDPWLADAERLGQRRGRCEALFDLLREAEAALAEAAASD
                     KAPSEAVVPTEAAPRRQAKAALREREEYAFAEAVLELDSARLAAYHELASGERRAAAD
                     LSLLARSALDGLYRAARALPDRRARRELRFLGFFYYDLRLRSIRRGAALPFAKALGFL
                     CALAELFLRGAGLLRLWERSAPGRRN"
BASE COUNT         2207 a         5713 c         6309 g         2273 t
ORIGIN      
        1 gccccgccgc ggggcgcggt tcctgccatg cctccctcct cgggtcccgg gccctgcttc
       61 gtcaatccgg tcctatcgtc gtgccgacga agggcttgcc ctccaggagg gcggcgaggt
      121 tctcgaggtt ccgccccgcg gcgcagacga cgcggaggcc gatctcggag gcccgcctcg
      181 tggcgatggg gtcgaagggc gcgttcgcgc ccggcttcca ctcgtcgccg acgagcttcc
      241 tgaagccttc ccaggagatt gcgtcgatcg gcttggcaga cgggtccttg cgggggtccg
      301 ccgtgtagac cttctcgatg ttggagaggt tgacgacctc cttcgcggcg aacttctccg
      361 cgaggaggac ggcgtcgtag tccgtggacc agccgggctt ccagccggcc gcgacgagta
      421 cgcggcccat gaagatggtg acggccgtcg ggtcggtgac cacgtcctgg ggggagaggg
      481 ggcccatgag ggcgcgcacg agctcggcgt tgaggcgggt cgccatgacg ccgatccagt
      541 cctgggccgc ggcgtcggca gcctcctcgg ggcgggccgc ctcgcggaag gccttctgcc
      601 agaggcgggc cggcccccct ccgcccacga ccaggatgag gcggcgggcg ggatcgacgg
      661 cgaggtgggc ctcgaccagg gcgcggaagg ccttgaggaa ggcgggatcg gggccttcgg
      721 gggcgacgat cgagccgccg agcgaaagga cggttgtcat gggctacctc cggcgcggga
      781 gcggcgaaag gaagagcccc cgggcctccg atgatagcgg ggaggccggg ggctcgtaaa
      841 gggcgcggaa ggacggagtg ggccgcccgc gccaagggcc gccgcgggcg gccgtccgtc
      901 cgggggcggc tccgctagaa gtccgaggag ccgaagaagt cctcgtcgct cccgaagaag
      961 tcgtcgccgc ccgaggagcc cggcatgccg gtcccgccct cgttgcggaa ggagtagagg
     1021 agctcggcgt agcggaaaag ggcgacgcgc cgcgagaggg tgtcgcgctc gtccctgacc
     1081 ttcctgagga agtcgcccgc cgtgagcccg ggccggagct cgcgccgctc caggctcgtg
     1141 ccggcgacca ggccgtcgat ctcgtccagg cggtagtaga cgcgctcgaa gatgacgagg
     1201 atcttcctga gcttctggag gtcgccgacg atggccttct tctcccgcat cacggcgggg
     1261 tcgaggccgc ggggcttcac tccggcgcgg tcctcgtcca tccacttctt gagcacctcg
     1321 ctcttctgga agggatcgaa ggcctggttg aagctcgagg agagctcggc gtagcgcttc
     1381 tcgtaggagg ccagggccct cgcgggatcg gccctgaagg cggccacctc gtcggcgacc
     1441 cacttctcgc ggaactgccg cacggaggag acggcgacgg ggaggtagcg gaggcgctcg
     1501 ccggggcgct tctccgcggc cttgccggcg gggacgggga actcctcctt gccgtccccg
     1561 aagctgacct cgccctccga gcagacgacc agggccgcct cgttgacgga gatcgcgacc
     1621 tcgaactcgg tgccgcggac gccggccacg gtgccgccgg tggtgacctg catggtcggc
     1681 gagccggaga gcttcttcac cttggaggcg actgagcccg cgagcaggtc gatctgggtg
     1741 cgcttctcgc ccttcgactc gtcgagcttg aggtagagcg cggacttggc cttcacggtg
     1801 atggtgccgc ccatgcctgt cgtcttgtcc atggcgatga cgaggaggcc gtcggaggag
     1861 gtcttcacga ggtccccgga gaggaggggg tcgtcgggat tcgggtcccc gaggctcttg
     1921 ccggcgcggg tgatcgtgac gccgccctcg acatagtcga tgcggccgac cggcgtcgct
     1981 cccgcggccc agagggccgg gacggaggag gcgaggatga gggcgaggag gagtcgcttc
     2041 acgttcatgc tccttggggg acgggccttc ccttcccgcg ccgagcctgg ggctccgcgc
     2101 gggggggctt cccggccgac ttgctaaata tagcgcgaaa ccccggggct ttcgagacgg
     2161 caaaataagg aaggggaggg gaggcgccgg gcgccgtccc ctccccttcg cgcggccgga
     2221 ctttgggcta gatcctgaag cccaggaacc agcgggagta ggcggtgaag gagcagccga
     2281 accaggtgtc ggagaagctc gaccccgcct tgcggcgctc ggggagggcg ggccagtcgg
     2341 ccaggtccac gtcgacgttg aggccgccca ggaggtggag ccttcccgcg agggggacgc
     2401 ccagggagag gcggaaggag gggtagggcc gctggctgaa ggagggctcg gtccactcct
     2461 cccgggcggc gcagtcggcc aggtggtcga ggaggggccc gatctcccgg gccgccgagg
     2521 cctcgaggtc gaggtagggc gagccgcgcc tgccgcccat cctggtgccg aggccgaagg
     2581 cggcaatcgc gctgtccgag gtctcgagcc agtccccgcg gggggcggag acgcggagga
     2641 cggtgaagag gctcctggag ccgttctgcc aggcgaagcc gagctcgtcg gagtcgggct
     2701 cccagtagcc gacgacgccg ctcacgccgt tgcgcgcgat gttgacgagg ccgagctgga
     2761 gcccgtcctc gatgtcgtcg gcgacgttga cgaggccgat ctggagcccc tcgacccgcc
     2821 gcgcctggtt gtagacgccg gccatctgca ggccttcgac atcgcccgcc gccaggttgg
     2881 ccacgcccgc catctgggcg ccgctcaccc ggccgcctgc gacgttgaag acgcccgcgc
     2941 cctggtagcc ctcgacgtcg cccgccacgg tgttgaagac gccggagccc tggaagccga
     3001 cgaggtcgcc cgcggcggtg ttgaagactc ccgagccctg gaagccctcc acgtcctccg
     3061 aggccgtgtt gaagaccccc gaggcctgga agcccgagag cctcctcgcg ttggcgaaga
     3121 cgccggcggc ctggaagccc tcgacgtcgc gggcgttggc gaagacggag gcggcctgga
     3181 ggccgtcaac gtcgcgggcc gtcgccatga tcaggccgag ctggacgtcg acgtcgaact
     3241 ccccgaaggg gagggagagg cccggcaggg ccgtgaagac gaagggatag cgcgggaccg
     3301 aatcggactc gccggactcg cctgccgtcg ggtagtaggg cgccggcggc gcctccgggg
     3361 cgggggcggc ttggggctgt tcggcctggg agccttggta ggcctcgcgg gccttgtcga
     3421 ggacgtagga ttcggagggt tcggttccgg actgggcgaa ggcgagggcg ctccccagga
     3481 gcagcgccgc tgccgtcgtg acggcggtct tgagcttcat ggttctcccc ttgtaggcga
     3541 gaggtgtcta ggtgcccccg ctacggggcg cacgggggat aaggtagcgc ggccggcggg
     3601 ggaaaaccaa gcattggata caaaaaagct cgtatacgaa actgaaatat cacgagcggt
     3661 ttccggtgcg tgggaggcgg cggataacgg ccgcaacccg gcggcgggcc atctgagcct
     3721 gcccggcggc gcatcgccct accggcccgc gatgcctcga cgcgggccga ctaagcggct
     3781 atcctcggcc gcatgggatt cctctcagcc tggcgcgagc ggcgccgcga gcgcgccgcc
     3841 ctcctccacc ccgtccccga cgagctctgg gactgggccg tgaaggagca ccgcatcttc
     3901 cgttggctct gcccggccga cctcgcgagg cttcgctccc tcgcggcggc cttcctggcc
     3961 gagaagacct tcgatcccgt cggcggcgcc gtcgtagacg aggagctccg cgtctcggtg
     4021 gcggcccagg cctgccttcc cctcctgggc ctccctggcg gcctcgactg gtaccgcgac
     4081 ttctcgacga tcatcgtcac gccccgggag tacgccgttc ggaagcgcga ctgggacgag
     4141 gcgggggtcg tccacgagta cgacgacgag ttcgcgggcg aggccttcga cctggggccc
     4201 gtcgccctct cccgcgtcga catcgaggcc tcgggctggg gcgacggcta caacgtcgtc
     4261 atccacgaga tggcccacaa gctggacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnga gcccgcgggc gagcccgccg ccacggccgg cccggccggg ccaggacccg
     4441 gcgctcggcc ggaggcctga gcgcggggag tcctagggct cccggcgccg ggcctcctcg
     4501 tccagggcgg cgaggaggtt ctcgcccagg gcgagggggc cggccgtctt gcggcggtag
     4561 aggtcgcggt ctgactcgcg gaagagctca tcgaagtcgg cctcgttggc gctgtggacg
     4621 cccagggtga cggagaaggg cacgggatgg gccgcgccgg ccttgtccag ggcctcctgg
     4681 atgcgggcgc gggctatctc cgcgtcctcc ggccccgtgg cgggcatcac gacgatgaac
     4741 tcgtcgccgc cgaagcgggc caccgagtcg gcctcccgcg cctccttccg gaagacggcg
     4801 gcgaccgcct tgagcacctc gtcccccgcg gcgtggccgt tctcgtcgtt gatgcgcttg
     4861 aaggcgtcga cgtcgcccac gacgacggag aggttccccg cgtagcgctt cacccgcgcc
     4921 gtctcccgct cgatcacctt ggccaggccg cgccggttca ggacgccggt gagcgagtcg
     4981 atctcggaga ggcgcctggc ctccgcgagg tagtagcact tctcgatggc tatcgcggcg
     5041 aagtcggcga tggtggagag gacgcggagg tcgaaggcgg agaagctgcc gccgtccagg
     5101 cggttcagga gctcgatcac gccgaagacc ttggagccgg tccgcagggg cacggcgatg
     5161 acgctcttcg tctcgaagcc gctgatctgg tcggcccggg gggcgaagcg ggagtcgccc
     5221 gcgacgtcct cgacgaaggc ggggaggccg cgccgcgcca cccagcccgc gatgccctcg
     5281 tccaggggga cgcgcttccc gcggaggtcg tcggcggcgc ggcccacgac gacggagaag
     5341 acgagctcgt cgcgctcggc gtcgacgagg aggacggacc agttgagggg gccgaagaag
     5401 gagccgacct gctccatcac ccggtccagg acgccgcgga tcgagttctc ggcgacgatg
     5461 ctcgtcccga tcgaggagaa gacggagagc ctgatgaggt cgtcgatgtc gggctcgcgt
     5521 tcggggtcgt tcatgtggcc tccgtctcaa ctatgccgcg ccttgggccg gctaacaagt
     5581 cggctcgggc gggaagggcg gcgccgggac ggcggaggcg cggcgtcgtg tcggccaagg
     5641 cgcggcgtcg cgacggccaa ggcgcgcctc gaaagcggac tggcgaaaaa agcgaaaagc
     5701 ggctatttac gacaagccgg ttcagggtcg atgatcgagc cgaggtcccg cggccttagg
     5761 cggcccgagg gaggttccca tggcttcgta cgaattctcc gagacggcgg ggcggatcgt
     5821 ccgctccgag atcagggaga tgctcaagtg gtccaggcgc cccgacacgg tgtccttcgg
     5881 cggcggcctg cccgaccctt cgctctttcc ggtggaggag ctggaggcca tctccgcccg
     5941 cgtcctcggg aagaagggct acctcgccct ccagtacggt cccaccccgg gggagcccgc
     6001 cgccgtcgag gccttcgtcc gccacatggc cgatttcggc gacgaggccc ttcccgccaa
     6061 cctctgcgtc acctcctcct cccagcaggc cctcgacctc ctgtccctcc tcttcgtcga
     6121 tccgggctcc cccgtcgtcg tggagatgcc ctcctacgtc ggggccctcc aggccttcgg
     6181 ccgggccggg gccgatttcc gcggcgtgcc cgtcgggcgc gacggcatgg acctggaccg
     6241 gctcgaggcc gagctgcgga ggctcgacgc ggagggcagg aagccgcgct tcgtctacgt
     6301 catccccgac ttccagaacc ccgccggctc gacgatgggg ctcgaggctc gcaggcgcct
     6361 cctctccatc gcccgcagac gcggcctcct cctcgtcgag gactctccct accgggagct
     6421 cgccttcgac ggccgcgccc tgccgagcct ctggagcctg gcggagggga agggcgtcgt
     6481 cctcctcaag accctctcca agatgctctt cccgggcttc cgcctgggct ggatctgcgc
     6541 cgaggaggcc ctggccgagc gcttcgagtg catgaagcag tccgtcgacc tctgcacctc
     6601 ctccttcgtc cagctcctcg ccgcggagta catcggcgag gggaggatgc gcgggaccat
     6661 cgagcgggcc cgggcgctct accgccccaa gcgcgacgcg atgctcgccg gcctggacgc
     6721 cgtcctcccg cccggctcgc ggcgaagcga gccggagggc ggcgtcttcc tctgggtcga
     6781 gctccccgag ggctgggacg cggcgcgggt cctccgcgcc gccctggccg agggcgtcgc
     6841 cttcgtcacg ggcggctcct tcttccacga gggctcgggg aggaactgca tgcggctcaa
     6901 ctactccttc ccctcggcga aggccatcgg gcggggctgc gagcggctcg gccgggccct
     6961 ggcggcggcg cggccctagg agctccgcct gttcctcata acgctcgacc ggtcctcccg
     7021 cgcgcccctc tcgcggcaga tagccgaggc ggtggtcaag ggcgtcgagt ccggcgccct
     7081 gcgcgccggc gacgccctgc cgtcgacgag gcgcctcgcg gaggagctcg gcgtctcgcg
     7141 cttcaccgcc gagcgcgcct acgaggagct ctgggccgag ggctacgtcg aggcccgctc
     7201 cggggcggcg acgcgggtca gggcgaggcc gccgcgggct tccggcgcgg cgcccgggga
     7261 ggccgccggg cgccgcgcgg gcggcggatc ccgtccggcc gccgaggggg agagggtcac
     7321 gggcggcgcg cggggacgga cctcgggcga ggcgcgcctt gagacgctcg tggatgcggc
     7381 gctcgaggag gcgctcggcg ccgccgccgg cctcaggtcc atccacgcgg cgcccgcggc
     7441 cggccggcct ggcgcggtcg acttcgcccg cttcaacctg gatccccgcc tctatccggt
     7501 cgccgccttc cgccgctccc tcgccgccgc cctcgcggag gatcccgcgc gggccctctc
     7561 ctacggtccc cccgagggcg atcccgccct ccgcgccctc atcgcccgcc gcatggccga
     7621 acacggggcg gcctgctccc cggagcggat cgtcctcgtg gacggcgccc tgcacggcct
     7681 ggacctcgtc ctgcgcctcc tcgtcccgcg gggcgggagc gtcctcgtcg aggccccgac
     7741 cttctccgga gccatcctcc tcgcgcgggc ctcgggcctc gaggtcgtcg gcgcggagac
     7801 ggacggcgag ggcctcctgc ccgcggcggc cgaggaggcc tacgaacgcg caagcgccgc
     7861 gggcaggcgc ccctccctcc tctacgtcac gccgagcttc cacaatccca cgggctcgct
     7921 ggcgggccag gggcggcgcg aggagatcct cgccttcgcg gcctcgcgcg gcctccccct
     7981 cgtggaggac tgcttcgagg aggagatagg cggccccggg gcgagggcca agcccatcgc
     8041 cgccatggac ggagaggggc ggacgatcta cctcgggacc ttctccaagg tgctcttccc
     8101 cggggtcagg ctgggctgga tcgcggcggc cccggccctg gcgcggcggc tcgccctcat
     8161 gcgccaggcg acgggggtct ccggcaactc cctcgtccag gcggccgtcg ccggcttcat
     8221 gtcctcgggc gcctacggcg cccacgtgag gcgggtcaac cgcgtcttct cccggcggct
     8281 cgcggccgcc gtctcggcct tcaggcgctt cgtgccggaa ggcgccgctc gcctcttcga
     8341 gcccgcgggc ggctaccttc tctggctgga gctccccgcg gggcgcgggg gcgccgcccg
     8401 cgccgaaacg gcccgagccc gggtagcccg cgccgaggcg gaggccgtcg aggcctgcgc
     8461 ggcggaggga ctcctcgtcg cgccgggcgc ctccttctgg ccgggcgagc cgccgggggc
     8521 cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgaggcc gaggctaggc
     8641 gacgcggccg gccggcggcc tcaggccagg ccgagcctgg cgagctcctt ctcgagcctg
     8701 gccggctcga cgggcttgag gaggtagccg tcggcgccgc agcggaagca ggccttcatg
     8761 taggaggcgg gatcgtccag gacggaggcg accaggatcc tcgcgtcctt ccgcgccagg
     8821 tcgccgacgc cggcggcgcc ggagctcagc tcctccaggg cgaaggccat ctccaggctc
     8881 cggatcgagc gcacgacctc gaagccgtcc atgccgggca tcacgaggtc gacgacgatg
     8941 aggtcgaagg gctcggctcc gggctccagg gagcgggcga cggcctccac ggcctcgtcc
     9001 ccgcccgcgg cgacggcgca ggagccgtat tgggcgagga tgtgctggct ggagcggcgc
     9061 tggaggaagt cgtcgtcgac gagaaggata gagagggcgc gggatcccgc ggcggcggcc
     9121 gagggccgga ccgaaggggc ggccgcggcg gagctcggct ccgcgcgggc ctcgaacggg
     9181 gccggcttgt cgacggccgg cggggccgcg gcgggccggg cctcggcgcg cggggcggcg
     9241 gtcggatgcg ccgcgaccgc gggccgcaag gcgggcgcgg cgggcgctgc cgcaggctgc
     9301 gaggctggcg cggcctgcgc ggcggcggac tgcgcggcgg gcgtgggccg cgaggcagct
     9361 cgaggcgcgg ccgtcgtcgc ggcaaggccc gacgcggcgg gagccggagc gcgggcggat
     9421 tccaaggcgg ccagaagctc aggccgcgcg gcctcctcct tggagccggc ggcgacggcg
     9481 gcgacgcggg acttgatggc gtccagggac tcgaggagga ggttcgtctc cgccctgccg
     9541 gagcggagtc cctcgcgctt gagcctggcg gccaggctct ccgcggcgtg cgcgatggcc
     9601 ggaatggcct ccagctcgaa catgcgggcg ttgcccttga tcgtatggag ggcccgcatc
     9661 agggcgtcga cggcctcggc gccgccgccg tcctcgagct cgagcgtcga gtcgacggcc
     9721 ttctgcacga gctcgtcgca ttcgaggaca aagcagccga aggcttcaag cagtatctcg
     9781 tccatttatt ttcccctgcg ggccggtcgc gaaacaggcc tcgcctaagg ctaagccttg
     9841 ggggcggcga ggtcaagggc gagggcggct tcagcccatg gagaggaggg cggagaggac
     9901 gtggcaggcc gcgcccgcga ggacgaagag gtgccagagc gggtggaacc aggggacctt
     9961 cttgagcgcg tagaacgcgg cgccgacggt gtaggcgacg ccgccggcga agaggaggtt
    10021 caaggaggcg ggcggcaggg ccgcgcgcag gggggcgaag gcgaagacga tgagccagcc
    10081 catcgcgacg tagcccaggg tggagaggag ccggaatttg tttatgaagg ccaccttgag
    10141 ggcggcgcct gcgacggcga gggcccagac cacggcgaag agggtccggc ccagggggcc
    10201 gcgtaggacg gtgaggcaga aggaggaata ggtgccggcg atcagggcgt agatggaggc
    10261 gtggtccagg acctcgagcc gcttcttggc ccggggggag gcgacgccgt ggtaggtggc
    10321 cgaggcgccg aagaggagga tgagggaggc cccgaagagg gcgtagccca ggacgcccag
    10381 tccgtcgctc cccgctgccg cggcgcggac gaggaggacg agggcggcta tggagaggag
    10441 ggccccgagg ccgtgggtcg cggcgttggc gatctcctcg gcgggcttgt agcgcctttc
    10501 gttcggcgtc gtgaattcgc agatgcttgc caattgatgc tccttgcgcc tggatagacc
    10561 gccgggagcg ccgggcggtt gcgggacggg cttgcgcagg ccccccgttt ccgcggacac
    10621 tggagtcagc gaccatgaac atcctgtccg cgctttcgct cctggcctgc gccttctacc
    10681 tcgagtcggg cctccgcgtc ctcttcctgg accggaaggg ccgcatcaac cggctctcct
    10741 tcgccctcta cctggacctc tgcctctggg ccttcgcggc ggccttcatc tacggcgcct
    10801 ccctccccga gacctccagg gcctggcacc tggccttctc gccgacctgg aacctcttct
    10861 tcggcttcgc cctccacttc tgcctcctcg ccgcgggccg ggacttccgg ctgactccct
    10921 ggcgcgcctt cctcctctac gcgcccggcc tcttcttcac cctctactcc aacctcttca
    10981 ggctcgagag cttcctcctg cacggcggct actggatgcc cgtcttcagg cccggactct
    11041 cctactggct cttcgtggcc tactactcgg tctacctcgg cttcggcctc ctcgccctgg
    11101 cccaggcctt cctgcgctcg aaggacgagc tcgagcggcg caggctcggc atcttcctcg
    11161 cctcgacctc cctccccttc gtcctcggct tcctctccga cagcgtcgcg ccggccctgg
    11221 ggctcgacct gccgaacctg ggcatcctgt ggatcgggat ctgggccttc ggcacgcgcg
    11281 tggccatgac gcgctacggc ttcttcgcgc ccgtccacga ggagatcgag cgccgcgtca
    11341 gggccgaggc gaggctcgcc gacgcgggcc gcctcttcga cgccttcctg gagaacagcc
    11401 tggacggcat cgtccttacc gacgcggagg gccgcgtgtc gcgctggagc gccgccatgg
    11461 aacggatctc gggactttcc gcggacgccg tcgtcggccg gccgatctgg gagatccagg
    11521 cctccatggc ctgggcgggc gacaaggagg agctttcgcg gagcatcgag gcggagatgc
    11581 gcaagctcct tgcgggtaag gaggccgggt ggctcgacga ggtgtcggag taccgcgtca
    11641 gggacggcaa gggcgaggag aagtacctcc aggcctcgag cttcgtgatc ccgatgtccg
    11701 gcggcaggca ggcggcggcc gccatcgtcc gcgacgccac cgagcggaag cgggccgaga
    11761 ccgaggccct cgaggagctc aggcggctcg acaacgcgca gaagatggag gccgtcggca
    11821 ccctggcggc cggcctggcc cacgacttca acaacatcct cacgggcgtc ctcgggacgg
    11881 tctctcttct ccggctggag gcgggcgaag gcggtcgggt ggagccggag gagctctcag
    11941 gcgggctcgg gataatcgag acccaggcct cgcgggccgt ggagatctcc cgccagctcc
    12001 tcgacctcac gaagcgccat ccggcggagg agaggccgac cagcctgggc gcggccatcc
    12061 agaaggccgc cgccctcacc aggggcggcc tggacccggc cgtcgagctc ctcgtcgaga
    12121 cgccgccgcc cgaggcctat gtcctctccg agggatcgga gctggagcgg gtgctcatca
    12181 acctgatcct gaactcgggc gaggccatga cctcgatgcg ggggcccggc gaggcgccgg
    12241 gcggcgccgt gcgcgtatcc gctttccgcg agggctcctt ctgggcgatc tcggtgaagg
    12301 acgagggggt cggcatcgcg cgcgaggccc tggcccgcgt cttcgacccc ttctacacga
    12361 ccaagggagg cggcaagtcc atggggctcg gcctctccat cgtctacaac atagcgaagc
    12421 gccggggagg ccgcgtcgag gtcgactccg cccccggcga gggcgccgag ttccgcgtct
    12481 acctgcccgc ctgcgagact ccctgagggc ggggcggccg gcctagttcg actcgatgag
    12541 cctgtccatc gccgcctggt tcatgcggaa gatgaggctg cggagcttct ccctcttgcc
    12601 ctcgtccagg gaggcggggg cgaacatgat gacgtgggcc gccctggccc cgtcgtgcct
    12661 ggccgcgacg aagccgtcga ccaggagccg cacgcccttc acggtcagct ggaggccgcg
    12721 caggatcgtc ccgggcttgt agctccgctc cccctcgaag gcgatcgcgc agccggccgc
    12781 ggagatgtcg ctcgcctcgc ccctgacggt ctccccgtcc agctcgacgg tgacctgggc
    12841 ggagccgggc gggcagaggg cgcggacgaa tttgcgcctg ccccgcgcct cattggcctc
    12901 cagggtcttg gccaggatgt cgatggtctt ggcggcgccg atcttgagga cgatgaagcc
    12961 gcagggcacg ccgatgtcca tgaggtactt ggaggaaagg tccttgtcct cgttgagggt
    13021 caggacgccg acgccgacgc ccgcggtctt ggggtccgac ctgagggact tcacgtagtc
    13081 ctcccaggcc tcctcctcga gctcggcgtc gatgttgacg aagacgaggg cgtccggctg
    13141 ggcggccagg acgcggcgga gccgggcgtg gtcgcggacg aggtagacct cgaactcgcg
    13201 gcgggagagt tcctccacga ccttggtgag gacaggcggc ggatacagga acacgacctt
    13261 cttgcccagg gcgccggctt ctatcattac ctctacgata gcgcccgcgg ccgacacgcg
    13321 caactggtgg gccgggcccg gcgctgccgt cccgcccggc gccggccgcc gtgcctggcg
    13381 gcgggccggt cgggcccggc ccggcctcgg cccctttgcc tagaagaggg actcgagctc
    13441 cccaaggccc tcggcggcca ggcccgcgcg gggcacgaag cgcagggagg ccgagttgat
    13501 gcagtagcgg aggcccgtgg gctcggggcc gtcctcgaag aggtggccca ggtggccgcc
    13561 cgaggccttg gccttcacct cgatccggcg catgccgtag gagaggtcct ccacgaggag
    13621 gagccgcgac tggtctatcg gcgcccagaa gctcggccag cccgagcccg actcgtactt
    13681 ctcccgggag gcgaagagcg gcgtcccgtc gatcgcgtcg aggtagacgc cctcctcgtg
    13741 gttgtcccag taggcgttct ggaagggcgg ctcggtggcc ccctgggcca ggatggcgaa
    13801 gcgctcgggg ccgagcctgg ccttgagctc ctcggtgggt tggacgtagc agacgttcgc
    13861 ggcgtcggcg gccttcacga tgggcatggc atctcctttc gcgagcgggg cggtggcggg
    13921 gcggggcgcg ccgggcacgg cggccgcggg cccggcggcc gggggcaatc ccggcgccgt
    13981 cgacgctgag ggccggggcg ctccgcaggc ggccagggcg gcgacgagtc cgagggcggc
    14041 caaggcggcg gtcaagcctg gggcggagag ccgctggcgg gcaagtccgg cccgcggctt
    14101 gtcggcgccg gcgggagacc ttcgctgatc gggcttaggg tacataaacg ataatatata
    14161 tagcggcgag ccgggaggaa agcgcgaagg gctggcaagg cgccggcggc caaagcgccg
    14221 gccgccaagg gggactcaag cggtccgtgg cctgggccgc ggcatggccg cggcccggac
    14281 cgtcgcccga gccttcttca gttccttcga cccggggcgg atctctccca gagacggagg
    14341 aggcccgcgc cccgcaggaa gagctccgcg agggcgcaga ggaagccgag ggccttggcg
    14401 aagggcaggg cggcgccgcg gcggatggag cggagccgca agtcgtagta gaagaagccg
    14461 aggaagcgca gctcgcgcct ggcccgcctg tcggggaggg cgcgggccgc gcgatagagt
    14521 ccgtcgaggg ccgagcgggc gaggagggaa aggtcggcgg cggcgcggcg ctcgcccgag
    14581 gcgagttcgt ggtaggcggc caggcgggcg gagtcgagct cgaggacggc ctccgcgaag
    14641 gcgtattcct ccctctcccg cagggcggcc ttggcttggc ggcgcggagc cgcctcggtg
    14701 gggacgacag cctccgaggg cgccttgtcc gacgcggcgg cctctgcgag cgccgcctcc
    14761 gcctcgcgga gaaggtcgaa gagggcctcg cagcggcccc tcctttggcc gagcctctcg
    14821 gcgtccgcga gccaggggtc gccccagtcg gcggggggct cctgtacggc attccggagg
    14881 ggggcgggga tgcggttctc cgtctccccg ggctcgaggt ccaggtcgat ggcccaggcc
    14941 gcctccaggg ccttccagta gcggagcatc ggcgcggccg cctggccgta ggccgcgccg
    15001 gcccagtcgg cgaggagggc ctccagggcc gccggcccgc ccgggcccag cgccgcgagg
    15061 cgggggaaga gccagaggtt gggcctgggc gcgaggggaa ggcggccgcc cgtgaggagg
    15121 atgccgacct tggacgcgcg ggccccgctt gagtcggggg actcgcccga gtaccaggcc
    15181 aggtccgcgg ccatggtcgt tcccaaggga gggaccgcgc ctttgaagag gagggcgtcc
    15241 tcgtagtact cgaagaccga ggcgcctcct ggggcggccg cgaaatcgcg gagcgccgtc
    15301 tcgaaggctc ctctcgaggc cgcgttgagg gctgagccgg actcgccgta gcccgaagcc
    15361 caactccgcc tgcgcggcgc ccagaggagc tcgaggttgc cgggcaggct cgcctcgccc
    15421 aggccgaggg ccttgagggc tcccgcgagc tcctccgtgt cgtggtaggc gaggaaggag
    15481 agcctcgcgc cgggcctggc ctccgcgagg gccgaggcca gggccagggc cgccttcagg
    15541 gactgggcgg cggcggagag gggcccgccc tccgcgtcga cgcaggccgg gcaggagcac
    15601 cagcccccgc ccgggaggtc gtcgggccag cagtggaaga cgcggacctc gggcctggcc
    15661 ttcgcgaagc gggcgaagct ttcggcggcc agggcgaggc tccgcgggtt cgaggggcag
    15721 aagttgcggt cggccttccg cccgccgcgc gtcatgcgaa agagctcggg ctcgcgcttg
    15781 gacaggctcc gcggcaggag ggaggagagg aggtggccgc cctcctcgag ctcgaggccg
    15841 aggcggccgg cgagggcggc gaggctggcg cgcccgccgt cgagccgcgc cgcggggcag
    15901 gcgcccaggg cgaggggccc ctcgatcgtg tggaggaaga ctcccgcgta gccgttccgc
    15961 gccgcgaaga ggaggtagtc cgccgccttt tccaggtagg cgccgtggcc gaggatgagg
    16021 acgggtcgga gggcgggccg gtccgagcgc ccggaggcgc gctcgaggcg gagcgagccc
    16081 ggcgccggga gccgttcgcc ctcggggccc ggctcgatcc agcggaagcc tcccgcctcc
    16141 aggaagtcgt agacgcaggc gaggaggccc tcgggcgagt cgccgtggag ctcgaccctc
    16201 gtgggcgagg cccgccaggc gaagccccgc ctggcggggg ccgcgaaagc cgcggcgtcc
    16261 ggcgctgcct ggccgcccgc gccgccatcc gcgcccgccg cgcctgaggc gccgcccgcg
    16321 cctccatgct cgcccgccgc gccgccgcgc ggcaggccct cgggccccgc gtcgaggagg
    16381 atgaggccct cggcttccga gccctctccc gcgacgatgg ggctcgatgc tatgcggcca
    16441 aggctggagg cgagctcggc ggccgcgaag cgggcgggat cggccgcgct ttcggggttc
    16501 gcgaggaggc gcgcgaggtc ttccggagcc gcgatgcccg agggggagga aggatcggcc
    16561 aggacgaggc gccaggggcg cgagagatct agttccatgg gaatggaggg tacgcgggcc
    16621 cagccggcgg ccgcaagggg ggctcaggcg gcgcgccgat cgggagcaca gggcatgccg
    16681 cggcgcgcat gccgccgcgc gc
//
LOCUS       DAOOSJ010000022        16396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2422_length_16396_cov_6.764274, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000022 DAOOSJ010000000
VERSION     DAOOSJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16396
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_16396_cov_6.764274"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1278)
                     /locus_tag="PLG14_02255"
     CDS             complement(88..1278)
                     /locus_tag="PLG14_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47982.1"
                     /translation="MTTSSSGAPDRDGRLFILATVLIGLGTCVNSAAFNNYLKDAYAL
                     DVARRTFLEFPRETPGFLVSLFVGILAALGETRIACAAGLAASAGMLALGWIPASFPL
                     MVASVFLYSSGGHVLMPLQNSIGMGFAAEGKEGAALGRIQFASTAAVLSGSAILLLLF
                     RFASLSYRAAFSAGAAAYAGAALVLLSMRPRRDSARAKRFVFRKEYSRFYVLSLLFGA
                     RKQLFITFGPWMIVDYFGQGVSLMTLLFFAVASLGLFVKPAVGRLTDRLGAKAVLSGE
                     ALLTIGVCLAYAFAPELLPRGAALLVVCLCYVADQASEAVTMTRAVYVKRIALAPDEV
                     APTLSLGISVDHVVAMFLPMLGGLAWRSGGSSGYKWVFLGGAVVAALNFLVARGIGSR
                     AEGA"
     gene            complement(1404..4616)
                     /gene="ileS"
                     /locus_tag="PLG14_02260"
     CDS             complement(1404..4616)
                     /gene="ileS"
                     /locus_tag="PLG14_02260"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOX47983.1"
                     /translation="MYKPVDPKVSFPKMEEDLVRFWEEGKIFEKSVSQREGAKDYVFF
                     DGPPFATGLPHFGHFVPGTIKDIIPRYKTMRGFRVERRFGWDCHGLPVENLIEKELGL
                     DSKTDIERFGVAEFNEACRASVLRYVKEWRATMTRAGRWVDFDHDYKTMEPDYMESIW
                     WVMKSLWDKGLVYEGHYILPYCPRCSTVLSNHELQLGGYKDVHDPAITIKFKAVSAPA
                     GIADAVRPAEGRTFLLAWTTTPWTLPSNLALCLGPDIDYVCVVDKGADGGVERYVLAE
                     SRLSSYYKEPGKLEIAWRKKGAELAGIEYEPLFPYFASAREQGAFRTVLGGHVTTEDG
                     TGIVHTAPGFGEEDHEVLRGTGVPTICPIDAECKFTSEVADYAGRFVKDCDKDILDRL
                     KAEGKLVKREQILHAYPHCWRCEHPLIYRAVGSWFVKVPAVKEKMLKANAKIRWVPSH
                     IQNGRFGKWLENARDWAISRNRYWGNPLPIWRCEDCGKTTCVGSRDELERLSGERPAD
                     LHKHFVDKITWKCECGGTARRIPEVLDCWFESGAMPYAQSHYPYENKEKFEAHFPADF
                     ISESLDQTRGWFYTLTVLAAALFDSPAFQNCIVSGMVLAADGKKMSKSQRNYTDPNEV
                     MNKFGADALRLYLMDSPVLKADDLCFTDEGVREVLKGVLIPLWNAYGFFVTYANIDGW
                     PENFGAAAGAAAGPAPANPLDRWILSVCEGMIEAVTAGLEAYDMPRAIAPIVDFIDLL
                     NNWYIRRSRRRFWRSEGDADKAEAYATLYRVLRRLSLTMAPIVPFVSESIWRNLRREG
                     EPESVHLALWPEHDASLRDEGLERKMDYARRAVSMGRALRVQHDIKIRQPLRAVHLVT
                     KDPAERAVLAEMEELLREELNVKEVAFREDEEELVEYSCKANFRVLGKELGKDMKAAA
                     EAIERLPGREVARILAGESVAVEAAGRRVELTAEKVEVRRSEKAGLSVLNEGSLTVAL
                     DTAIDRALVEEGWVRDLVRGVQTLRKEAGLEVTDRIRLVVAGDEELKAAFGSFRDFVA
                     SETLASSLSWAEGPAAPGSGGAFSEIEAGDKAWRAAVAKA"
     gene            4786..6066
                     /locus_tag="PLG14_02265"
     CDS             4786..6066
                     /locus_tag="PLG14_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47984.1"
                     /translation="MNTTPRARRALLLGLSASVLLLGSGCASLGDDLFLIKGLSPREK
                     AEALVEAGAAAYKVRLVAKGDTGAIEEVARYFQAALRYDPANREASRYALLVEDYRAA
                     RLRAAVKEGNALLKKQKRSEAEDLALVAAARRAVELDGSDDDAKRLLRDTGPVREGLV
                     TAFLERADAAMARATPEASAATREKAYVDAYTAASKALYVEPGQPKAYRMQVSLRSEV
                     SDVLKSRLKGVDALVKKGAFPEARAQLGPAKDLDARLSGAFASDIASAEYDLYYRWAQ
                     KLAAAKDYAGAEEKADLALAAKRGSEAQALKKSLADRRSQAELGASFGAGLANVDRYI
                     AARDLPRAQRVLAGLSRQSLDAAKKAQLNERRDALREALAEVYERGVRAYKAESFKEA
                     IAAFEAVIAVDESYEEVASYLEKSRAKQKLLDQF"
     gene            6090..8219
                     /locus_tag="PLG14_02270"
     CDS             6090..8219
                     /locus_tag="PLG14_02270"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX47985.1"
                     /translation="MKTPEAKVLRAHAPEIAYSALVLAWFVLPLFSRVEGALSPALLP
                     FGLAAARGGRFSLLGALFAWVPAGLALWKLAAPFLRERLSAARAGSGLALALSAAESA
                     LVVAAQASHAAVLAGRPAYFRAQGALGYAAFALSIAFNAWCLVLLIRSASRRNEGYEE
                     YARFRESSGEYVRGIAAFAGRRGIQRRITAVFTAFILALVAILSGFLLRDFSGTILKA
                     VIDKGEGLAERAASVVKSSVGDRIAVEDYFAIESRKNLEETFPFESLSYYRRDPKSGK
                     LAVLESTVARLVGAAAPAGIEKLEETGYSIDRKTGRIEFRAPVVLSKTFLGYVSVVYE
                     NEVIFEPYFRTQVKVWIIASFFVYASVFLTYLIGRSIVFPILFLRMSVNSISKRLSGM
                     IKGEAKVSADSLRYEDRVTTKDEIKKLSLEIGDMANVIRGVIPYISASTLKHSESAAT
                     VSERRELAFLFTDIRGFTTLCEGLSPEAVVALLNRYLELQTEAIHAAGGEVDKFVGDE
                     VMAMFEGPDRELKACRAGLAIRKAMAEERERARAESQAEISIGIGINSGPVVFGSVGA
                     RERMDFTSIGDTVNLAARLEGANKTYGTKSLVTEAVKEKVEDTILLREIDLLTVKGKT
                     KPARIYELIQERSGSSAKLERLKSGFEAGLAAYRARRWAQAEKAFAALVKEYGDEPSA
                     VFLRRVELFKASPPPADWDGVFAMKVK"
     gene            complement(8249..>8800)
                     /locus_tag="PLG14_02275"
     CDS             complement(8249..>8800)
                     /locus_tag="PLG14_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47986.1"
                     /translation="RALAAGSPIRGLAAALNGTSSLVLSLMEEGLSLEAAVGRAQELG
                     MAEADPSADLDGLDAAVKLAILSRAVLGEALPLQRIARESVRLASPERLARAAAAGRR
                     LRAIGRLSRLPGGGVEAAVRLEEVPPASPLAAAGGGAALVYDCERSGEVAVSGRGAGP
                     LETASAVYRDLVSLAAAGASGRG"
     assembly_gap    8801..8900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8901..10010)
                     /locus_tag="PLG14_02280"
     CDS             complement(8901..10010)
                     /locus_tag="PLG14_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47987.1"
                     /translation="MRRPAKGAAPQAAATAAPRRAAVLFAAAAAFLVAVAPAASFAEG
                     GEAAGPAFTLGGAVSQRSYLQASLVDGADFPEASLGYSGMTGLETTLRAASGPARAEA
                     SLEATVASGAAAGAGPEPLVTASVRTLYASLRGGAFKASLGRQVLNFGKGALYSPVDL
                     FAAAEYSPLAISRRGTDALRLTAALGPLALAEAVAAPKADPARGLYALRAAGYLAGLA
                     DASLVGAWNGAEEAWMAGGDAKVDLPFASVYAEAVFAFPSGGGGPEPRAAVGFDASLG
                     DLVLAAEYFYDSRPAAGTATGAHNLYASLAYGLSDYAKLAASASVDLEAGTGSGGLVA
                     SLEVEQGTFLGLSATLTRTGSSTTAAAGLSLELRF"
     gene            complement(10007..10870)
                     /locus_tag="PLG14_02285"
     CDS             complement(10007..10870)
                     /locus_tag="PLG14_02285"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX47988.1"
                     /translation="MNLPSCHDGAARALPAAGSARPSARPSNCSADIGARGRPRAAAL
                     LALLAALAAAPAAAQGPGLPSPEELLRRVDANQAFDSIEYSGRMEILSGGKTRVKTMR
                     ASALGDSKALVEFTNPEDRGVRMLKVGKELWMYFPKEGDTVKISGHLLKEGMMGSDLS
                     YEDALESDSLLEKYSASVLREEEAEGRPAWVLELKAKAPTANYDRRLLWIDKERYVAL
                     KGEMYARSGKLLKTSRVLEVARVAGRWFPVRSEISSAIKKGSTTTIAMGDLKLDAPIP
                     ESRFSLAELTK"
     gene            complement(10867..12114)
                     /locus_tag="PLG14_02290"
     CDS             complement(10867..12114)
                     /locus_tag="PLG14_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX47989.1"
                     /translation="MRILGMAFRNVGRNRRRSALALSSVAISILLVMVMDGLMTGFLG
                     SIVKNYTKNETGHVNIATEGYRARERFMPIDETIASSREIVDALAGLPGLEGKVQSVA
                     ERIRFGVVLSSGPRSKAALGIAGDPEAERSLLMLDRSVKEGAYLAAPGDAILGERLAE
                     DLGLGLGDTLKVVTQKADSGLGFKRFRVAGIFRTGVNSLDEAVFQVGIADARELLGLP
                     EGASQQLLVILKDYRDSDKAAGLMAGALGAKGLRGLSVQPWTAMGDFLKLVKMAASVY
                     YWMYAVVAFLGAFIITNIMMMVVLERRKEIGVLKSMGMPSREVLALFLAEGTMLGALG
                     AAVGAGLGLAFNAVFSKLGFDFTSAMSGFDWPMDNIVYPRPNVLAALAFFAMGTAVAA
                     IVSLLPARSAARMNPVEAIRSAV"
     gene            complement(12127..13386)
                     /locus_tag="PLG14_02295"
     CDS             complement(12127..13386)
                     /locus_tag="PLG14_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX47990.1"
                     /translation="MGTLLKIAYRNVLRHKRRTAITGVVMSVGIGLFIAADSMLVGMD
                     RLTIDNMVDYTESFLKARSPEYVANLAGTPLEHGVPAPEAAAEALMAADPRIKAVTPR
                     TRFVARASNYSDEIPVLATAVDPERDAAVFRTAGSVGAGRWLGPGAERGVVLGSGLAK
                     ELGLGLGDYVLVAASTVYENVNADEYEIVGLVETPIPEINKGALYISYADAEELLGLG
                     GLVTELCASAERPPTLSALLKSSEEAAAKAGAALPGLKVDSIGELAKGYLAMRQMKGK
                     FSQILIFVLLLIAAVGIVNTILMSVYSRIREIGVLRAYGMRPKEIRRLFTIEGLLVGL
                     VGSLAGLALGLALDWYLVSVGIPMEAMLGDIDMGGIPLAGTLRGEWNPGTAAFGFVFG
                     ILVAFLSARIPARRAARLEVTDALRFV"
     gene            complement(13391..14077)
                     /locus_tag="PLG14_02300"
     CDS             complement(13391..14077)
                     /locus_tag="PLG14_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX47991.1"
                     /translation="MAILSLQKIRKVYDKGGSPVVALGGVDLEIEKGEYLAVVGPSGS
                     GKTTLLNILGCLDAPTEGRIVYAGRELRGLGERDLSEYRKEHVSFIFQSYNLIPVLTV
                     LENVELPLVIERRLRPAEVRAKARELIAAVGLSDKEGRFPRELSGGQEQRVAIARALV
                     KDPIVVLADEPTANLDSHTAEEIVELMRRINAERSTTFIFSTHDRLVMEHARRIVTLR
                     DGAVTGDERR"
     gene            complement(14086..14406)
                     /locus_tag="PLG14_02305"
     CDS             complement(14086..14406)
                     /locus_tag="PLG14_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOX47992.1"
                     /translation="MKRAIGELGLDKLIHERARLMILTYLASSSEAETGFTELRDGLG
                     FTAGNLSTQLRVLEEGGLVDIGKSFRSNKPYTGVRLTPRGKRALEDYLAELEVIVASL
                     KATR"
     gene            complement(14403..15071)
                     /locus_tag="PLG14_02310"
     CDS             complement(14403..15071)
                     /locus_tag="PLG14_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47993.1"
                     /translation="MDKAEIERLLDDVENLRKAIRKNDPLLREVADSRLYALLTLPFG
                     LFILVFFLGTHFLLGEGGSFAGLPEAWRLAFWAFLAVFFVAGGVAKVVIIGRKAKALD
                     AEAGFGSVVKAAYGGALLHVNVPALVAMAVVSAYAALSGHPWLILPATAVFFAFVCNV
                     LGALVGQKEYFLTGWYGLLSGLASLFFVEGAPYLWVAVVYGGIFLVFGAASLVSRRRR
                     AGGR"
     gene            15235..16107
                     /locus_tag="PLG14_02315"
     CDS             15235..16107
                     /locus_tag="PLG14_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOX47994.1"
                     /translation="MPDNDEDAPGFYRTELGGEGAERLVLRFSSADASILPSEGEGIG
                     LELELRGPKAALADWRPNVRRVEGILVLAEAEGPVKVVELRARIPAAIRDVEFHTRSG
                     DAEVSGLKIGVLAETETGRIRVRDAASLEARSATGDIEVERTGACDLSVGSGRVRCLF
                     PQDKVVARSKSGDIDIDDAEGDLYLETASGEISVTRPKGRVRAQSGAGDIEIEAPDPF
                     GGGEATSVSGRIGFILGGADLELRAETLSGRLVTPQGEIGTPAGPRRCALSVGKGGRR
                     LHAKSVSGDIEIDY"
     gene            complement(16175..>16396)
                     /locus_tag="PLG14_02320"
     CDS             complement(16175..>16396)
                     /locus_tag="PLG14_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX47995.1"
                     /translation="AKLELDRMVAAAATSAAAIAVPAAAKASAPAAAAPHPAAAAIAV
                     PAAGARPAAAALAASAPRVAEAAASAAAM"
BASE COUNT         2437 a         5641 c         5853 g         2365 t
ORIGIN      
        1 ccgcggcccc ggatccctcg ggtcggtgcg gcccgacagg ccgcgccgca acccgcgccg
       61 cggcccgtag cccgcgccgc ggcccgccta ggctccctcc gcccgactcc cgatgccccg
      121 agcgaccagg aagttgagcg ccgccacgac ggccccgccc aggaagaccc acttgtagcc
      181 cgacgagcct ccgctcctcc acgcgagccc tccgagcatc gggaggaaca tcgcgaccac
      241 gtggtcgacc gagatcccga gggagagggt cggcgccacc tcgtcggggg cgagggctat
      301 ccgcttcacg tagacggccc tcgtcatggt cacggcctcc gaggcctggt ccgcgacgta
      361 gcagaggcag acgacgagga gggcggcgcc ccggggaagg agctccggcg cgaatgcgta
      421 ggccaggcag acgcctatcg tgaggagggc ctcgcccgag agcacggcct tggcgccgag
      481 cctgtccgtg agcctgccca cggccggctt cacgaagagg ccgagcgagg cgacggcgaa
      541 gaagaggagg gtcatgaggg agacgccctg gccgaagtag tcgacgatca tccagggtcc
      601 gaaggtgatg aagagctgtt tcctggcccc gaagaggagg gagaggacgt agaagcggga
      661 atactccttc ctgaagacga agcgcttggc cctcgcgctg tcgcggcggg ggcgcatgga
      721 gaggaggacg agggcggcgc ccgcgtaggc ggccgcgccg gcggagaagg cggcgcggta
      781 gctcaaggag gcgaagcgga acaggaggag gaggatggcc gagcccgaga ggacggcggc
      841 cgtcgaggcg aactggatcc tgcccagggc ggctccctcc ttgccctcgg cggcgaagcc
      901 catgcctatc gagttctgga ggggcatgag gacgtggccg cccgaggagt agaggaagac
      961 ggaggcgacc atgaggggga aggaggcggg gatccagccg agggcgagca tgcccgccga
     1021 ggcggcgagg ccggccgcgc aggctatcct cgtctcgccc agggcggcga ggatgccgac
     1081 gaagaggctc acgaggaagc ccggcgtctc gcgggggaat tcgaggaagg tgcgccgcgc
     1141 gacgtccagg gcgtaggcgt ccttgaggta gttgttgaag gcggccgagt tcacgcaggt
     1201 gcccaggcct atgaggacgg tcgcgaggat gaagaggcgg ccgtcccggt ccggggcgcc
     1261 gcttgagctg gtcgtcatgc gcagaggtat agcacgaagc cagccttcga tgcgcctaaa
     1321 acgagggccg ccccggtctt tccggggcgg ccctccatgc tcgacttggt ccgcgggccc
     1381 gcggtacggc cgcggcgcgg cctttaggcc ttcgcgaccg cggcccgcca ggccttgtcg
     1441 ccggcctcga tctcggagaa ggccccgccc gagccgggcg cggccgggcc ctcggcccag
     1501 gagagggagg aggcgagggt ctcgctcgcg acgaagtccc tgaaggaccc gaaggcggcc
     1561 ttgagctcct cgtcgccggc gacgacgagc ctgatgcggt ccgtcacctc gagcccggcc
     1621 tccttcctga gggtctggac gccgcgcacg aggtcgcgga cccagccctc ctcgaccagg
     1681 gcgcggtcga tcgccgtgtc cagggccacg gtgaggctgc cctcgttgag gacggagagg
     1741 ccggccttct cgctccgcct gacctccacc ttctccgccg tgagctcgac ccgcctgccc
     1801 gccgcctcga cggcgacgga ctcgcccgcg aggatcctcg cgacctcgcg gcccggtagc
     1861 cgctcgatcg cctcggcggc cgccttcatg tccttcccga gctccttgcc caggacgcgg
     1921 aagttcgcct tgcaggagta ctcgacgagc tcctcctcgt cctcgcggaa ggccacctcc
     1981 ttcacgttga gctcctcgcg gaggagttcc tccatctcgg ccaggacggc ccgctcggcg
     2041 ggatccttcg tgacgaggtg gacggcgcgc aggggctggc ggatcttgat gtcgtgctgg
     2101 acgcgcaggg cccggcccat ggagacggcg cggcgggcgt agtccatctt gcgctctagg
     2161 ccctcgtcgc ggagcgaggc gtcgtgctcc ggccagaggg ccaggtgcac ggactcgggc
     2221 tcgccctcgc ggcgcaggtt ccgccagatc gactcggaga cgaagggcac gatgggcgcc
     2281 atcgtgaggg agaggcgcct caggacccgg tagagggtcg cgtaggcctc cgccttgtcg
     2341 gcgtcgccct cgctccgcca gaagcggcgg cgggagcgcc ggatgtacca gttgttgagg
     2401 aggtcgatga agtcgacgat gggcgcgatc gcccgcggca tgtcgtaggc ctcgaggccc
     2461 gccgtgacgg cctcgatcat gccctcgcag acggagagga tccagcggtc caggggattg
     2521 gcgggggcgg gccccgcggc ggctccggcg gccgcgccga aattctccgg ccagccgtcg
     2581 atgttcgcgt aggtgacgaa gaagccgtag gcgttccaca gggggatgag gacacccttc
     2641 aggacctcgc gcacgccctc gtccgtgaag cagaggtcgt ccgccttgag gacgggggag
     2701 tccatgaggt agagccggag cgcgtcggcg ccgaacttgt tcatcacctc gttcgggtcg
     2761 gtgtagttgc gctgggactt ggacatcttc ttgccgtcgg cggccaggac catgccggag
     2821 acgatgcagt tctggaaggc cgggctgtcg aagagggccg ccgccaggac ggtgagggtg
     2881 tagaaccagc cccgcgtctg gtcgaggctc tcggagatga agtcggccgg gaagtgggcc
     2941 tcgaacttct ccttgttctc gtaggggtag tggctctgcg cgtagggcat ggcgcccgac
     3001 tcgaaccagc agtccaggac ctcggggatg cggcgcgcgg tcccgccgca ctcgcacttc
     3061 cacgtgatct tgtcgacgaa gtgcttgtgg aggtcggcgg ggcgctcgcc cgagagcctc
     3121 tcgagctcgt cgcggctccc gacgcaggtg gtcttcccgc agtcctcgca ccgccagatc
     3181 gggagggggt tgccccagta gcggttgcgg ctgatggccc agtcgcgggc gttctccagc
     3241 cacttgccga agcggccgtt ctggatgtgg gagggcaccc agcggatctt ggcgttcgcc
     3301 ttgagcatct tctccttgac ggcggggacc ttcacgaacc aggagccgac ggcgcggtag
     3361 atgaggggat gctcgcacct ccagcagtgg ggataggcgt ggaggatctg ctcgcgcttg
     3421 acgagcttgc cctcggcctt gagcctgtcc aggatgtcct tgtcgcagtc cttcacgaag
     3481 cggccggcgt agtcggcgac ctcggaggtg aacttgcact ccgcgtcgat ggggcagatc
     3541 gtcgggacgc ccgtgccgcg caggacctcg tggtcctcct cgccgaagcc gggcgcggtg
     3601 tggacgatgc ccgtgccgtc ctcggtggtg acgtggccgc ccaggaccgt gcggaaggcg
     3661 ccctgctccc gggcggaggc gaagtagggg aagaggggct cgtactcgat cccggcgagc
     3721 tcggcgccct tctttctcca ggcgatctcg agcttcccgg gctccttgta gtaggaggag
     3781 aggcgcgatt cggcgaggac gtagcgctcg acgccgccgt cggcgccctt gtcgaccacg
     3841 cagacgtagt cgatgtccgg ccccaggcag agggccaggt tcgaggggag ggtccagggc
     3901 gtcgtcgtcc aggcgaggag gaaggtccgg ccctccgcgg ggcggacggc gtcggcgatc
     3961 ccggcggggg cggacaccgc cttgaacttg atcgtgatgg cggggtcgtg cacgtccttg
     4021 tagccgccga gctggagctc gtggttcgag aggacggtcg agcagcgggg gcagtagggg
     4081 aggatgtagt ggccctcgta gacgaggccc ttgtcccaga ggctcttcat gacccaccag
     4141 atggactcca tgtagtcggg ctccatcgtc ttgtagtcgt ggtcgaagtc gacccagcgg
     4201 ccggcccggg tcatggtggc gcgccactcc ttcacgtagc ggaggacgga ggcgcggcag
     4261 gcctcgttga actcggcgac gccgaagcgc tctatgtcgg tcttggagtc taggccgagc
     4321 tccttctcga tgaggttctc cacggggagg ccgtggcagt cccagccgaa gcggcgctcg
     4381 accctgaagc cgcgcatcgt cttgtagcgg gggatgatgt ccttgatggt tcccggaacg
     4441 aagtggccga agtgggggag tcccgtcgcg aagggggggc cgtcgaagaa gacgtagtcc
     4501 ttggctccct cccgctgcga gaccgacttc tcgaagatct tcccctcttc ccagaatcgg
     4561 accaagtcct cctccatctt cgggaagctg accttcgggt ctaccggctt gtacatgatg
     4621 ctcctcctgg cgggcgttcc gttcggcgcc agtatgccat agagggggca caatttccat
     4681 agcgcgggcc cccgcctcat tgagccccgg ggcttttttc attatccttc ccttaacgac
     4741 gagggcggcc gccccaggcg cctccccata cgacggagcg aagcggtgaa cacgactccc
     4801 cgcgcccgca gggcgcttct cctcggcctc tcggcctcgg tcctcctcct cggctccggc
     4861 tgcgcgagcc tgggggacga cctcttcctc atcaagggcc tctccccccg ggagaaggcc
     4921 gaggccctcg tggaggccgg cgccgcggcc tacaaggtcc gcctcgtggc caagggcgac
     4981 acgggggcca tcgaggaggt ggcccgctat ttccaggcgg ccctccgcta cgaccccgcc
     5041 aaccgcgagg cctcccgcta cgccctcctc gtcgaggact accgcgccgc ccgcctccgc
     5101 gcggccgtga aggagggcaa cgccctcctc aagaagcaga agcgcagcga ggcggaggac
     5161 ctggccctcg tggccgccgc gaggcgggcc gtcgagctcg acggctcgga cgacgacgcg
     5221 aagcggctcc tccgcgacac gggcccggtc cgcgaggggc tcgtcacggc cttcctggag
     5281 cgggccgatg cggcgatggc cagggccacc cccgaggcct cggccgcgac gagggagaag
     5341 gcctacgtcg acgcctacac cgcggcctcg aaggccctct acgtcgagcc cggccagccc
     5401 aaggcctacc ggatgcaggt ctccctgcgc tccgaggtct ccgacgtcct caagtcgagg
     5461 ctcaagggcg tcgacgccct cgtcaagaaa ggcgccttcc cggaggccag ggcccagctc
     5521 ggccccgcga aggacctgga cgcgcggctc tcgggcgcct tcgcctcgga catcgcctcg
     5581 gccgagtacg acctctacta ccgctgggcc cagaagctcg cggccgcgaa ggactacgcc
     5641 ggggccgagg agaaggccga cctggccctg gccgcgaagc gcgggagcga ggcccaggcc
     5701 ctcaagaagt ccctcgccga ccggcgctcc caggccgagc tcggcgcctc cttcggggcg
     5761 ggcctggcca acgtggaccg ctacatcgcc gcccgcgacc tgccccgggc ccagcgcgtc
     5821 ctcgcgggcc tctcccgcca gagcctcgac gccgcgaaga aggcgcagct caacgagagg
     5881 cgcgacgcgc tgcgcgaggc cctcgccgag gtctacgagc gcggggtccg cgcctacaag
     5941 gccgagtcct tcaaggaggc gatagccgcc ttcgaggccg tcatcgcggt ggacgagtcc
     6001 tacgaggagg tggcgagcta cctcgagaag tcccgcgcga agcagaagct cctcgaccag
     6061 ttctagaaga cgcgatgcgg aggaacgcca tgaagacgcc tgaagccaag gtcctgcgcg
     6121 cccacgcgcc cgagatcgcc tactcggccc tcgtgctcgc ctggttcgtc ctgcccctct
     6181 tctcgagggt cgagggcgcc ctctctcccg ccctcctccc cttcggcctg gcggccgccc
     6241 ggggcgggcg tttctccctc ctcggcgccc tgttcgcctg ggtcccggcg gggctcgcgc
     6301 tctggaagct cgcggctccc ttcctccgcg agaggctcag cgccgccagg gccggctcgg
     6361 gcctcgccct cgccctctcc gccgcggagt cggccctcgt cgtggcggcc caggcctccc
     6421 acgccgctgt gctcgccgga aggcccgcct acttccgggc ccagggcgcc ttgggctacg
     6481 cggccttcgc cctctccatc gccttcaacg cctggtgcct cgtcctcctc atccgctccg
     6541 cgagccgccg caacgagggc tacgaggagt acgcccgctt ccgcgagagc tcgggcgagt
     6601 acgtccgggg catcgccgcc ttcgccggcc gcaggggcat ccagcgcagg atcacggccg
     6661 tgttcaccgc cttcatcctg gccctcgtcg ccatcctctc cggcttcctc ctgcgcgact
     6721 tctcggggac catcctcaag gccgtcatcg acaagggaga gggcctggcc gagcgcgccg
     6781 cgagcgtcgt caagtcctcc gtcggcgacc gcatagccgt ggaggactac ttcgccatcg
     6841 agtcccggaa gaacctggag gagaccttcc ccttcgagtc cctgtcctat taccgccgcg
     6901 accccaagtc gggcaagctc gccgtcctgg agagcacggt cgcgcggctc gtcggcgccg
     6961 cggccccggc cggaatcgag aagctcgagg agacgggcta ctccatcgac cggaagaccg
     7021 gcaggatcga gttccgcgcc cccgtcgtcc tctccaagac cttcctgggc tacgtctccg
     7081 tcgtctacga gaacgaggtg atcttcgagc cctacttccg gacccaggtg aaggtctgga
     7141 tcatcgcctc cttcttcgtc tacgcctccg tcttcctcac ctacctcata ggccgcagca
     7201 tcgtcttccc catcctcttc ctcaggatga gcgtcaactc gatctccaag cggctctccg
     7261 gcatgatcaa gggcgaggcg aaggtctccg ccgactccct gcgctacgag gacagggtga
     7321 cgaccaagga cgagatcaag aagctctccc tcgagatcgg cgacatggcg aacgtcatcc
     7381 gcggcgtcat cccctacatc tccgcctcga ccctgaagca ctccgagagc gccgcgaccg
     7441 tctccgagcg gcgcgagctc gccttcctct tcaccgacat ccgcggcttc accaccctct
     7501 gcgaggggct ttccccggag gcggtggtcg ccctcctcaa ccgctacctg gagctccaga
     7561 ccgaggcgat ccacgccgcg ggcggcgagg tggacaagtt cgtgggcgac gaggtgatgg
     7621 cgatgttcga ggggcccgac cgcgagctca aggcctgcag ggcgggcctc gcgatccgca
     7681 aggccatggc ggaggagcgc gagcgggcca gggccgagtc ccaggcggag atctccatcg
     7741 gcatcggcat caactccggc cccgtcgtct tcggctccgt cggcgcgagg gagcgcatgg
     7801 acttcacgag catcggggac acggtgaacc tcgcggccag gctcgagggc gccaacaaga
     7861 cctacggcac gaagagcctc gtcacggagg cggtgaagga gaaggtcgag gacaccatcc
     7921 tcctccgcga gatcgacctc ctgaccgtga aagggaagac caagcccgcc cgcatctacg
     7981 agctcatcca ggagcgctcc ggctccagcg ccaagctcga gaggctcaag tccggcttcg
     8041 aggcggggct ggccgcctac cgggccaggc gctgggccca ggccgagaag gccttcgccg
     8101 ccctcgtgaa ggagtacggc gacgagccga gcgccgtctt cctccggcgc gtcgagctct
     8161 tcaaggcctc cccgccgccc gccgactggg acggcgtctt cgctatgaag gtcaagtagg
     8221 agcccggccg cccgggcggc tctccggatc agccccggcc cgacgctccg gccgcggcca
     8281 gcgacacgag gtcccggtag acggcgctgg ccgtctccag ggggccggct ccccgtcccg
     8341 agacggcaac ctccccgctc cgctcgcagt cgtagaccaa ggcggccccg ccgcccgccg
     8401 ccgcgagggg cgaggcgggc gggacctcct cgagccgcac ggcggcctcg acgccgccgc
     8461 ccgggaggcg cgagaggcgg ccgatagcgc ggagccgcct cccggccgcc gccgcccggg
     8521 ccagccgctc cggcgaggcg agcctgacgc tctccctcgc gatccgctga aggggcagtg
     8581 cctctccgag caccgcgcgg gagaggatgg cgagcttgac cgcggcgtcc aggccgtcca
     8641 ggtcggcgga gggatcggcc tcggccatgc ccagctcctg ggctcggccg acggcggcct
     8701 ccagggagag gccctcctcc atgagcgaga ggacgaggct cgaggtgccg ttcagggccg
     8761 cggccaggcc gcggatcggc gagcccgccg cgagggccct nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn ctagaaccgg agctcgagcg agaggccggc ggccgccgtc
     8941 gtcgaggagc ctgtcctcgt gagcgtcgcg gagaggccca ggaaggtccc ctgctcgacc
     9001 tcgagggagg cgacgaggcc gcctgagccc gtccccgcct cgaggtcgac ggaggccgag
     9061 gcggccagct tcgcgtagtc cgagagtccg taggcgaggc tcgcgtacag gttgtgcgct
     9121 cccgttgccg tccctgcggc cgggcgggag tcgtagaagt actccgcggc caggacgagg
     9181 tcgcccaggg aggcgtcgaa gccgacggcg gccctgggct cgggcccgcc gccgcccgag
     9241 gggaaggcga agacggcctc cgcgtagacc gaggcgaagg gcaggtcgac cttggcgtcg
     9301 ccgcccgcca tccaggcctc ctccgctccg ttccaggctc cgacgaggga ggcgtccgcg
     9361 aggccggcca ggtagcccgc ggccctgagc gcgtagaggc ctcgggcggg gtcggccttg
     9421 ggggcggcga cggcctcggc cagggcgagg gggccgaggg ccgcggtgag gcgcaaggcg
     9481 tcggttccgc ggcgcgagat ggcgaggggc gagtactccg ccgccgcgaa gaggtcgacg
     9541 gggctgtaga gagccccctt gccgaagttg aggacctgcc gtcccagact ggccttgaag
     9601 gcgccgccgc ggaggctcgc gtagagggtg cgcaccgagg cggtgacgag gggctcgggc
     9661 cccgcgcccg ccgccgcgcc cgaggcgacg gtcgcctcga gcgaggcctc cgcgcgggcc
     9721 ggtccgcttg ccgcgcgcag ggtcgtctcc aggcccgtca tccccgagta gcccaggctc
     9781 gcctccggga agtccgcgcc gtcgaccagg ctcgcctgga ggtacgagcg ctgcgagacc
     9841 gctcccccga gggtgaaggc cggcccggcc gcttccccgc cttcggcgaa ggaggcggcc
     9901 ggggcgaccg cgaccaggaa ggcggccgcc gccgcgaaca ggactgccgc gcggcgcggc
     9961 gcggcggtcg cggccgcctg gggcgcggcg cccttcgccg gccgcctcat ttcgtgagct
    10021 cggccaggga gaagcgggac tcggggatgg gcgcgtccag cttgaggtcg cccatcgcga
    10081 tggtcgtcgt cgagcccttc ttgatggcgc tcgagatctc ggagcgcacg gggaaccacc
    10141 tccccgccac gcgcgcgacc tcgaggaccc ggctcgtctt gaggagcttg ccgctcctcg
    10201 cgtacatctc gcccttcagc gcgacgtagc gctccttgtc gatccacagg agacggcggt
    10261 cgtagttcgc cgtcggggcc ttggccttga gctcgaggac ccaggcgggc ctgccctcgg
    10321 cctcctcctc gcggaggacg gaggcggagt acttctcgag gagggagtcg gactccaggg
    10381 cgtcctcgta ggagaggtcg ctgcccatca tgccctcctt gaggaggtgg ccggagatct
    10441 tcaccgtgtc gccctccttc gggaagtaca tccagagctc cttgccgacc ttgagcatcc
    10501 gtacgccccg gtcctcggga ttcgtgaatt cgacgagggc cttcgagtcg cccagggcgg
    10561 aggcgcgcat cgtcttcacg cgcgtcttgc cgccagagag gatctccatg cggccggagt
    10621 actctatgga gtcgaaggcc tggttcgcgt ccacgcggcg gaggagctcc tcgggcgagg
    10681 gaaggcccgg gccctgggcc gccgcgggcg cggcggccag ggccgcgagg agggcgagga
    10741 gggcggcggc ccgtgggcgg ccgcgggcgc cgatgtccgc ggagcagttc gagggccggg
    10801 ccgagggccg ggccgagccg gcggcgggga gggcgcgcgc ggcgccgtcg tgacaagagg
    10861 gaaggttcat actgcgctcc ttatggcctc gacgggattc atgcgggccg cgctcctcgc
    10921 gggcaggagg gagacgatgg ccgcgaccgc cgtgcccatc gcgaagaagg ccagggcggc
    10981 caggacgttg ggccgcgggt agactatgtt gtccatgggc cagtcgaagc ccgacatcgc
    11041 ggaggtgaag tcgaagccga gcttggagaa gaccgcgttg aaggcgaggc ccaggcccgc
    11101 cccgacggcc gcgcccaagg cgccgagcat ggtgccctcc gcgaggaaga gggccaggac
    11161 ctcgcggctc ggcatgccca tggacttgag cacgcctatc tccttcctcc gctccaggac
    11221 gaccatcatc atgatgttcg tgatgatgaa ggcccccagg aaggcgacca ccgcgtacat
    11281 ccagtagtag acgctcgcgg ccatcttgac gagcttgagg aagtcgccca tcgcggtcca
    11341 gggctggacg gagaggccgc ggaggccttt cgcgcccagg gctcccgcca tgaggccggc
    11401 cgccttgtcc gagtcgcggt agtccttgag gatcacgagg agctgctgcg aggcgccttc
    11461 cgggaggccc aggagctccc gggcgtccgc tatgcccacc tggaagaccg cctcgtccag
    11521 ggagttcacg ccggtgcgga agatccccgc gacgcggaag cgcttgaagc ccaggcccga
    11581 gtcggccttc tgggtcacca ccttgagggt gtcgcccagg cccagcccca ggtcctcggc
    11641 gagcctctcg ccaaggatcg cgtcgcccgg cgccgcgaga taggcgcctt ccttgacgct
    11701 ccggtccagc atgaggaggg aacgctccgc ctcggggtct cccgcgatgc ccagggccgc
    11761 cttggagcgg gggcccgagg agaggacgac gccgaagcgg atccgctccg cgaccgactg
    11821 gaccttgcct tcgaggccgg gcagccccgc cagcgcgtcc acgatctccc gggacgaggc
    11881 gatcgtctcg tcgatgggca tgaagcgctc cctggcgcgg tagccctccg tcgcgatgtt
    11941 gacgtggccg gtctcgttct tggtgtagtt cttgacgatc gagcccagga agcccgtcat
    12001 gagcccgtcc atgaccatga cgagcagtat cgagatcgcg acggaggaga gggcgagggc
    12061 gctcctccgc ctgttcctgc cgacgttcct gaacgccatt cccaaaatgc gcatcgcgac
    12121 gctcctttag acgaacctga gcgcgtcggt gacctcgagc ctggcggccc gccgcgcggg
    12181 gatccgcgcg gagaggaagg cgacgaggat cccgaagacg aagccgaagg ccgcggtgcc
    12241 ggggttccat tccccgcgca gcgtgccggc gagcggtatc cctcccatgt cgatgtcgcc
    12301 cagcatcgcc tccatcggga tgccgacgga gacgaggtac cagtcgaggg ccaggcccag
    12361 ggccaggccc gccaggctcc cgacgaggcc cacgagaagg ccctcgatcg tgaagagccg
    12421 cctgatctcc ttcggcctca tgccgtaggc gcgcaggacg cctatctcgc ggatcctcga
    12481 gtagacgctc atgaggatcg tgttcacgat gcccacggcc gcgatgagga ggaggacgaa
    12541 gatgaggatc tgggagaact tgcccttcat ctggcgcatc gcgaggtagc cttttgcgag
    12601 ctcgccgatc gagtcgacct tgaggcccgg cagggccgcc ccggccttcg ccgccgcctc
    12661 ttccgaggac ttgagcaagg cggagagggt gggcgggcgc tcggcggagg cgcagagctc
    12721 ggtgacgagg ccgcccaggc ccaggagctc ctcggcgtcg gcgtaggaga tgtagagggc
    12781 gcccttgttg atctccggga tgggggtctc cacgaggccg acgatctcgt actcgtccgc
    12841 gttcacgttc tcgtagacgg tgctcgcggc cacgaggacg tagtccccga gcccgaggcc
    12901 gagctccttc gcgaggcccg agcccaggac gacgccgcgc tccgcccccg gccccagcca
    12961 gcggcccgcg ccgacggaac cggccgtcct gaagaccgcc gcgtcgcgct cggggtcgac
    13021 ggccgtcgcg aggacgggga tctcgtcgga gtagttggag gcccgcgcga cgaagcgggt
    13081 gcggggcgtc accgccttga tccgcggatc ggcggccatg agcgcctcgg ccgcggcctc
    13141 gggagcgggg acgccgtgct ccaggggggt gcccgcgagg ttcgcgacgt actccgggct
    13201 cctcgccttc aggaaggact ccgtgtagtc gaccatgttg tcgatcgtga gcctgtccat
    13261 gccgacgagc atcgagtcgg ccgcgatgaa gagcccgatg ccgacgctca tgacgacgcc
    13321 cgtgatggcg gtgcgccgct tgtggcggag cacgttgcgg taggcgatct tcaggagggt
    13381 gcccacggcc ctacctcctc tcgtcgcccg tgacggcgcc gtcgcggagg gtgacgatcc
    13441 tccgggcgtg ctccatcacg agcctgtcgt gcgtggagaa gatgaaggtc gtgctccgct
    13501 cggcgttgat ccgccgcatg agctcgacga tctcctccgc ggtatgggag tccaggttcg
    13561 ccgtcggctc gtcggccagg acgacgatgg ggtccttcac gagggcgcgg gcgatggcta
    13621 cgcgctgctc ctggccgccc gagagctccc gggggaagcg gccctccttg tcggagaggc
    13681 cgacggccgc gatgagctcg cgcgccttcg cgcggacctc cgcgggcctc agcctgcgct
    13741 cgatgacgag ggggagctcg acgttctcca ggaccgtgag gacggggatc aggttgtagg
    13801 actggaagat gaagctcacg tgctccttcc ggtattccga gaggtcccgc tcgccgaggc
    13861 cgcggagctc gcggcccgcg tagacgatcc gcccttccgt cggcgcgtcc aggcagccga
    13921 ggatgttgag gagggtggtc ttgcccgagc ccgagggccc gacgacggcg aggtactcgc
    13981 ccttctctat ctcgaggtcg accccgccca gggccacgac ggggctgccc cccttgtcgt
    14041 agaccttgcg gatcttctga aggctcaata tcgccatggt tccccctagc gcgtcgcctt
    14101 gagcgacgcg acgatgactt cgagctccgc gaggtagtcc tcgagggcgc gcttgcccct
    14161 gggcgtgagc ctcacgccgg tgtagggctt gttggacctg aagcttttcc cgatgtccac
    14221 gagccctccc tcctcgagga cgcggagctg ggtggagagg ttgccggcgg tgaagcccag
    14281 gccgtccctg agctcggtga agccggtctc cgcctcgctc gaggaggcga ggtaggtgag
    14341 gatcatgagc ctggcccgct cgtggatgag cttgtcgagg ccgagctccc cgatggccct
    14401 cttcatcttc cgccggccct tctccgcctg gagacgaggc tcgcggcgcc gaagaccagg
    14461 aagatcccgc cgtagacgac ggcgacccag aggtagggcg ccccctcgac gaagaagagg
    14521 gaggcgaggc cggagaggag gccgtaccag cccgtgagga agtactcctt ctggccgacc
    14581 agggcgccca ggacgttgca gacgaaggcg aagaagacgg ccgtggcggg caggatgagc
    14641 cagggatggc ccgagagggc ggcgtaggcg gagacgacgg ccatggcgac gagggcgggc
    14701 acgttcacgt ggaggagggc gccgccgtag gccgccttga cgacggagcc gaagccggcc
    14761 tcggcgtcca gggccttggc cttgcgccct atgatgacga ccttggcgac gcccccggcc
    14821 acgaagaaga cggccaggaa tgcccagaaa gccagcctcc aggcctcagg gaggcccgcg
    14881 aagctccctc cctcgcccag gaggaagtgg gtcccgagga agaagaccag gatgaagagc
    14941 ccgaagggga gggtgaggag ggcgtagagc ctcgagtcgg ccacctccct gaggaggggg
    15001 tcgttcttcc gtatggcctt cctgaggttc tcgacgtcgt ccaggagccg ttctatctcc
    15061 gctttgtcca tagtgtctcc ataaacaagt ttgtaccgca aacaagatga agtataatcc
    15121 tccgggtttt tttgtcaata gccccaaaac gactcgtccg aaagggggag atgtggtatc
    15181 atcatgcgcc gtatcttcgg gcttccggcc ggaagcccgg cgctggaggt tgccatgccg
    15241 gacaatgacg aagacgcccc cggtttctac cggaccgaac tcggagggga aggggccgag
    15301 cgcctcgtcc tccgattctc ctcggccgac gcctccatcc tcccctccga gggcgagggc
    15361 ataggccttg agctcgagct ccgcggcccc aaggcggccc tggccgactg gcggcccaac
    15421 gtgcggcgcg tcgagggcat cctcgtcctg gccgaggccg aggggccggt gaaggtcgtc
    15481 gagctcaggg cgcggattcc cgcggccatc cgcgacgtcg agttccacac ccgctcgggc
    15541 gacgccgagg tgagcggact caagatcggg gtcctcgcgg agaccgagac cggtcggatc
    15601 agggtgcggg acgcggcctc cctggaggcc cgctccgcga cgggcgacat cgaggtcgag
    15661 cgcacgggcg cctgcgacct ctccgtcggc tcgggccgcg tccgctgcct cttcccccag
    15721 gacaaggtcg tggccaggag caagtccggc gatatcgaca tcgacgacgc cgagggcgac
    15781 ctctacctcg agaccgcctc cggcgagatc tccgtgaccc gccccaaggg cagggtcagg
    15841 gcccagagcg gggcggggga catcgagatc gaggcccccg accccttcgg cggcggcgag
    15901 gcgacgagcg tctccggccg catcggcttc atcctgggcg gagccgacct ggagctcagg
    15961 gccgagaccc tctccggccg cctggtcacc ccccagggcg agatcggcac ccccgccggt
    16021 ccgcgccgct gcgccctctc cgtcgggaag ggcggccgcc gcctccacgc caagagcgtc
    16081 tccggcgaca tcgagatcga ctactgaccg tcttcccggc gcctgcggcg cggggcaaag
    16141 cggcggcggc cttgcggggc cgccgctttt tttgtcacat cgcggcggcc gaggctgccg
    16201 cctcggcgac gcggggggcg cttgccgcca gggcggccgc ggccggccgg gccccggcgg
    16261 ccgggacggc tatagcggcg gccgcaggat gaggcgccgc ggccgcgggc gcggaggcct
    16321 tagcggcggc cgggacggct atagcggcgg ccgaagtcgc ggcggcggcc accatccggt
    16381 cgagctcgag cttggc
//
LOCUS       DAOOSJ010000023        16361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2426_length_16361_cov_5.457623, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000023 DAOOSJ010000000
VERSION     DAOOSJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 16361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16361
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_16361_cov_5.457623"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1110
                     /locus_tag="PLG14_02325"
     CDS             49..1110
                     /locus_tag="PLG14_02325"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOX47996.1"
                     /translation="MSAAFDPAGTRIAASDWTTGKAVIIDVAGGKVAGEVELRGRPAG
                     VAWSPDGSRLYVAECDGGAVAEIDPAAMKVARRLAVGLRPAGIAIAPSGKVLLAANGD
                     SHSVSIVEIESGKELKRIPAEREPFAISITADGKSAIVSNLLPATRATDIDHAAALTV
                     IDLEKLEKAGCVRLPPGSTDVRQTATSPDGKVLYVARFSGGSILRVRLDDLAVETFWP
                     ADGGAKRSLALDASRGRLYATDMDRGSLFVLDLGTGRLVAEVPLGWNPNGLALSPDGS
                     RVYACTRGPNGAEGYERPGPLAGELVAVDAESLAVVGRQWGGNQPTGLAVAPDGKRLV
                     FTDFLDRRVEAYDTDMRER"
     gene            1112..1960
                     /gene="nudC"
                     /locus_tag="PLG14_02330"
     CDS             1112..1960
                     /gene="nudC"
                     /locus_tag="PLG14_02330"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOX47997.1"
                     /translation="MNPTPERAYLFRGAEILVPLSGGPWLEPGALDGLGLERRREYPA
                     RGAACLAAALSAAPAHPAGYAPGRQASQAPELVELPGLPPLKPLPFRAALAELPHEEA
                     SRASKGLALLNWLGAARFCGGCGAELADDGTEGYAAGARRCPACSRVYYPRVSPAVIL
                     LVRREGKILLAHNAAFPAGRYGLIAGFVEPGESLEEAARRELLEETGIEVGELRYSAS
                     QPWPFPDSLMVGFEAEWRSGEGKPDGVELTELLWRGPDELPSIPPPGSIARRLIDGFL
                     RSAASS"
     gene            complement(1938..2696)
                     /locus_tag="PLG14_02335"
     CDS             complement(1938..2696)
                     /locus_tag="PLG14_02335"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOX47998.1"
                     /translation="MPLCQTCGVELDGTATVCPLCLSRVQAAAGDRPSPPAEGPGAGA
                     ATAGGNASTESGAAVAEARGGSAGPRGRALAVEALSVVFGIAALLCAGIDLYAEGRLG
                     WSPVVLVGLAMGWLLACMPMVLRGKPWLVFAVLCPGELALLLLLDLADGGGAWFLRYG
                     LPIYLASVGALTAVVVLAAASRRKGLNLLALAAAGAAALCLAVEAVLSLAAGGPFVLR
                     WSLFVAAAALPASGLLFYAHYRLFRRPLRRKLRI"
     assembly_gap    2840..2939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2973..3662)
                     /locus_tag="PLG14_02340"
     CDS             complement(2973..3662)
                     /locus_tag="PLG14_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX47999.1"
                     /translation="MDDRDIALLAASPLFAGADLLELGSYLRTAPVSVRSYPPGSTIL
                     LAGSAYEELRVILAGEASAEMADGEGRAVVVETLKAPDAVATAVLFAPERRLPVSVSA
                     RSEARLASIPRGILLGAAARFPAVLEALLLDMGSRLAFLAAKYRSASFATLGERLADW
                     LLERAERDGSGLLSVTLPETKERLAAALGVARPSLSRELSRMEDRGLLAVRGRRIEIL
                     DERGLRRALSR"
     gene            3728..5137
                     /locus_tag="PLG14_02345"
     CDS             3728..5137
                     /locus_tag="PLG14_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOX48000.1"
                     /translation="MDFSWKIVIDAGAIAMALLLATLLRAKVRFFQKYLIPNALTAGF
                     ILLPVYNFLLPSLGYAQHRLGELVYHLLNVSFIAMTLRASPPKVKGHKGGGVLGMSTG
                     ILLGYSTQALLGLGLTLLFLPRIFPSFGLFLPLGFALGPGQAYAIGKGWEAMGFDGAP
                     SIGLAFAAIGYLWACFGGVFLINHGLKKGWLKGEGLAAFKDKAMMSGVVPLDAEKPVG
                     ARLSTDSEAIDSFSFHAAAVVFTYLLSYVFLLGLTRLLELAGKPGHDLAVNLWGINFI
                     FSSITAIAVRKVLDRLRLGRLLDDGTLSRISGFSVDFMVAGSLGAISLIFVGKFWAEI
                     LLMSTVCGLMTLVVVPWFCSRMFRDYEFERMLMIYGVSTGTLSTGLALLRVMDPEFKT
                     SVASDYMLSAGLTFVLAVPFILSINLPAKAATTGSMSYYWIMMGVAAAYLAYVLASYA
                     WIARRKSLAKPASLWFKEP"
     gene            complement(5124..6335)
                     /locus_tag="PLG14_02350"
     CDS             complement(5124..6335)
                     /locus_tag="PLG14_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX48001.1"
                     /translation="MNATHRYVPRNYRWFNKLLKWTLGAWLRRAYRVESRGGEFLKAL
                     KPPFIVVANHVTTRDPFILGPLIRQPVYWVTGDGAMRTPLLGKILRFVGSIPKTKAIP
                     DMQTINWIVEVTRKRKGVVGVFPEGQATWDGRSLPVLPATAKLLKLLKIPVVAARISG
                     GYSSLPRWTWKRRPGRFVVDWKLALEPAELKALTAEEIVARLESAIGHDECAYLEAEP
                     QEFESRRMAEHLELSLFMCPACGSIGKLKSFRSRFNCLACGEALALDGRYRFRRLGGG
                     EPAFTTIRDWDAWQEKTFAAAAARAAAERPEAPFLSDPGALLLRGRKTRPLRRVRAGT
                     LLLYRDRLELATHFGSRLSFPLAEIEGANVYKRNLLEFYVGRTLFQARFPLRATSARK
                     WCMGIEALKVP"
     gene            complement(6487..6849)
                     /locus_tag="PLG14_02355"
     CDS             complement(6487..6849)
                     /locus_tag="PLG14_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOX48002.1"
                     /translation="MVDLESISRIVSLLNRLDERLKDAQAVGLAEAVVLSSLGRGIRS
                     PSEIAAATGIGASNLSRILKTLEERGLVERNIDDKDKRRFRFALTPAGHETLVAVEGS
                     RLEIPEELLEAYETLGKD"
     gene            7159..8079
                     /locus_tag="PLG14_02360"
     CDS             7159..8079
                     /locus_tag="PLG14_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX48003.1"
                     /translation="MAKRIVGKDGVLKGILTGVIASGHVLLEGVPGLAKTLAVKTFAE
                     ISGLDFSRIQFTPDLLPADITGTLVYEQATGRFTPRRGPLFANVVLADEVNRAPAKVQ
                     SALLEAMQEGQVTIGEASHALPEPFFVLATQNPIEHEGTYPLPEAQLDRFLLKVVADY
                     PSAEEEAAIVRRVADRSSAPVDRVLGAAEIALLRKAAESVAVDEAIVGYIVRLVRATR
                     PGPESPAELRDTARWLEYGASPRASIHLFRCARISALLDGRSHVLPGDVKASAPEVLR
                     HRLVLSYQAEADGVDADAAIARILSAVPLP"
     gene            8086..8970
                     /locus_tag="PLG14_02365"
     CDS             8086..8970
                     /locus_tag="PLG14_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX48004.1"
                     /translation="MDRDELHGRIRRLLFVSPALSSAFSRGDFRSVFRGRGMDFEALR
                     EYGEGDDARLIDWNVSVRFARPFVRTYREDRSLTLFLLVDVSASMEEGSGELSKRDAA
                     VLSASLLAYAAQLRGMPVGALVFAGGAIRYFEPRRGKAHALALIEAAVGAHAEGRASA
                     GGTDLGGAFERAGRLLKRRSLVMALSDFDAEGWAPSLALLARRHDVVAVRVTDGLDRG
                     LPAYGAFGAADAEGRGPAWLPLGSRSFRARWAKRGEARREACRALCAEAGARLLEIDT
                     SDDPARRLLEFFDRRRRP"
     gene            8967..10031
                     /locus_tag="PLG14_02370"
     CDS             8967..10031
                     /locus_tag="PLG14_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48005.1"
                     /translation="MSRRASDHGVGAGRAGAGRGSSALQRAARRGEPSGHAAGGEQPR
                     RAARSGEPSRRPAPRGLAAAILALCLSALPAGAEPLGLVRLGFAPARFGAGEEVEAVA
                     LLEAGSAEPEAFALKAGGGLPEPGPGADPELRELRLSKTAGGWELRARFVPWTPGKGL
                     LPPLSVRGLALPGLPYTVDSVLAPGDREPAPPKPQRDPPGTALYLYGFAGLVLVLVLA
                     GFWAAAVLVPGARRLLARRRAAQARRALERSLAYLERGLGGSSPADFYAALARSLRLY
                     LAARLLPEAAALTPAEIGALPAARFPEAGIRDDAAAVLGEADLARFAGLNPGLDAMRA
                     ALERSARLAGSAEEALDALL"
     gene            10018..11016
                     /locus_tag="PLG14_02375"
     CDS             10018..11016
                     /locus_tag="PLG14_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX48006.1"
                     /translation="MLSFERPALLAFLLLVPLLAFARRLFRARLSAPRFPVAALPGEA
                     PPAPPLARRALFFLAQALLWLGLALLVLASAGPARVSRRVLYLSRGNEIVFVLDVSPS
                     MAAEDFEPTRLAASKAIIDEFLSSRRNETVGLVAFGGEAALVCPPTLDYGVLRRRLGD
                     LKPGLLGEGTALGAGIGVALAHSGGKGAPARYVILLTDGENNAGPMAPATAAALAARR
                     GVSVSVIGVGSRGEVPVTYVDPDTGQRRSGTYLSGFDRASLESLARIGGGSYFAAESP
                     EALERAFSAVSEDSVSLAMTRSVSTEDSLARPLLALALAALVLARLLGLLSGGDFL"
     gene            11013..12107
                     /locus_tag="PLG14_02380"
     CDS             11013..12107
                     /locus_tag="PLG14_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX48007.1"
                     /translation="MSFERPELLWLLLLAPLELLLALRRAPRLRASLELLSGPRRREA
                     QGARYAAASVYGTAASVVLAAASALALAGPAWGVKAVASERSGLEVAVVLDVSRSMEV
                     REGGASRLEGAKSLLRGLLRQAPGASFSLVAAKGDAVLLVPMTEDLEALDSALDYADP
                     ETTTAAGTDLGKGIRAGLESFTAGSGASRAGADRAGVNRALLLLSDGGDKGGEALKAA
                     EEARKRKTRLLAVGLGGAEALPVPGPSGGPLLDAKGDAVRSALEPTLLRAVAAASGGR
                     YLEASDSGAAQALRAELAELGRGGKRVEYESKDRSSFFILLALAALVVRVVASILAFA
                     AADRSTVEAGRAAPGRPAAADSPAAERTGR"
     gene            12104..12679
                     /locus_tag="PLG14_02385"
     CDS             12104..12679
                     /locus_tag="PLG14_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48008.1"
                     /translation="MRRRLALLAAAAALLATGCGALRDYDAVIKGNRLHGRGRYQEAA
                     ASYLSAGKGAFGAVLSYDLANVYARLGEVQAAAELYAEARKAGDASLSAAAFYNEGLA
                     LYEKGRYEEAYRAFRSALSLDPRDGEARRNLEIAWRDWKKSALSPPERAAPSSRSQGE
                     GDEELRLLRRLETGRYKPGGTTPQLPNPEDY"
     gene            12681..14570
                     /locus_tag="PLG14_02390"
     CDS             12681..14570
                     /locus_tag="PLG14_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48009.1"
                     /translation="MRSARLKAPLLGLAVLVGLGAAGPSRAAPASSAPAAAAAAPAFV
                     YRLSVEPESPMAGEPFAVVLRVEGLTRVSLDKLVLDPLLSLEGSSSRPRFELSTGMRE
                     TETRLELKALAPGSLLIDSIELAGLEGRLAVGPLRIEVRGAAKAEAGPLWSWEAPDSA
                     YRYSTFGLRLAGPAGAAEDAFASFDAPPGLALEPAGDGLSWICTALEEGRLRLPEASV
                     EAGPDSGKAQARSILVRPLPPGAAATRAVGDFELRFEGPPSGKGRAGEALGFRLTLSG
                     SGNHPALRFPGVAASLDGKPLPEGSLSPRRSEALKQGPRGYEGSSVLELDLVPPAAGL
                     LRVSPSPFAYVTPEGRSLELRVDPIEVRVAPAAPGGAEEGRGDPFAEAVAALEALPEG
                     ARGAAGAALIAAAASWKAGERGKALAQAYGAARRFPFDARAAKAADAASLAVGAGPRQ
                     RDAYAPPAFFAWIGSGLFALGAGLALAGRPRRRGLGRPGTGGAKAGAEGRRAEQGGAK
                     AGSGGRKAGRGAKAADARRRAKAARGGPGGRPVLGAAGGLLLATALAAFVLAGLALRE
                     RRVEYAVAWTDRAYPVPSAKASGTVLVDRGASGRVLGRAPGFVGLRLADGSVAWVPAE
                     AVYLY"
     gene            14579..15145
                     /locus_tag="PLG14_02395"
     CDS             14579..15145
                     /locus_tag="PLG14_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOX48010.1"
                     /translation="MDFGDILDEWDKRSSKPGGLKGADEVERRLREAEASRLALLKEE
                     ERRKAAAKARARYSLESWLDEHGVEDKDGKDEPQAGGQASREAEARRLKDLKPQARLD
                     LHGMRALEAEAALASFLEASSRAGLEKVLVVTGKGMHSQGEPVLGKTARRVLESSPFA
                     GRFGQADPSEGGSGALWVIVRRKGYFSR"
     gene            complement(15130..15348)
                     /gene="infA"
                     /locus_tag="PLG14_02400"
     CDS             complement(15130..15348)
                     /gene="infA"
                     /locus_tag="PLG14_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOX48011.1"
                     /translation="MAKEEAIEVEGIVKESLPNTMFRVELPNGHVILAHLSGKMRKHY
                     IRIVPGDKVKVALSPYDLNRGRIIYREK"
     gene            15573..15929
                     /locus_tag="PLG14_02405"
     CDS             15573..15929
                     /locus_tag="PLG14_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOX48012.1"
                     /translation="MDKDFIERMRSSLVAQKKEILDTLAATNADFRAIVEEMDPKDFA
                     DVASDDIDRKMIEALGSQDIKRVRAIDAALSRIQQGHYGLCMKCGKKIPIDRLTAIPY
                     AVLCIECQKGEERRNR"
     gene            complement(16024..>16361)
                     /locus_tag="PLG14_02410"
     CDS             complement(16024..>16361)
                     /locus_tag="PLG14_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOX48013.1"
                     /translation="NPGGRLFDEEDPAYLAAAFEAAGLLEGKGIVAELNLGGLARGKT
                     KEPYPSLPILRELKRLRVPITFCADAHDTRHVGVHLDAARELARAAGYESVAVLTKGS
                     WIEVGIDET"
BASE COUNT         2118 a         6061 c         5767 g         2315 t
ORIGIN      
        1 tccgcccgat cgggggatgc gccggcggca ggaccttacc ggtcaccctt gagcgcggcc
       61 ttcgatcccg ccggcacccg gatcgccgcg agcgactgga ccacgggcaa ggcggtcatc
      121 atcgatgtcg ccggcgggaa ggtcgccggc gaggtcgagc tgcgcgggcg gcccgccggg
      181 gtcgcctggt cccccgacgg ctcgcgcctc tacgtggcgg agtgcgatgg cggcgccgtc
      241 gccgagatcg accctgcggc catgaaggtc gcccgccgtc tggccgtcgg gctccggccc
      301 gcggggatcg ccatcgcccc ctcggggaag gtcctcctcg ccgccaacgg cgactcgcac
      361 agcgtctcca tcgtcgagat cgaaagcggg aaggagctga agcggatccc cgccgagcgg
      421 gagcccttcg cgatctccat caccgccgac gggaagtcgg ccatcgtctc gaacctcctc
      481 ccggccaccc gcgcgacgga catcgaccac gccgcggccc tgacggtgat cgacctggag
      541 aagctcgaga aggccggctg cgtccgcctc ccgccggggt ccaccgacgt gcgccagacc
      601 gcgacgagcc ccgacgggaa ggtgctctac gtcgcccgct tttcgggcgg ctccatcctc
      661 cgcgtccgcc tggacgacct cgcggtggag accttctggc ccgccgacgg cggcgcgaag
      721 cggagcctcg ccctggacgc ctcgcggggc aggctctacg cgaccgacat ggaccggggc
      781 agcctcttcg tcctggacct cggcaccggc cgcctcgtcg ccgaggtccc cctgggctgg
      841 aacccgaacg gcctcgccct ctcgccggac gggagccgcg tctacgcctg cacgaggggg
      901 cccaacgggg cggagggcta cgagaggcct gggccgctcg cgggcgagct cgtcgccgtc
      961 gacgccgaga gccttgcggt cgtcggccgg caatggggcg gcaaccagcc gacgggcctc
     1021 gccgtcgcgc ccgacgggaa gcggctcgtc ttcacggatt tcctcgaccg ccgcgtcgag
     1081 gcctacgaca ccgacatgag ggagcgatga gatgaacccg acacccgagc gcgcctacct
     1141 cttcaggggc gcggagatcc tcgtgcccct ctcaggcggc ccctggctcg agcccggggc
     1201 cctggacggg ctcggcctgg agcggcggcg cgagtacccc gcccgaggcg cggcctgcct
     1261 ggccgcggcc ctgtccgccg cgcccgccca ccccgcgggc tacgcgccgg gcaggcaggc
     1321 ctcccaggcc cccgagctcg tcgagcttcc cggccttccg ccgctcaagc ctttgccctt
     1381 ccgcgcggcc ctcgccgaac tgccccacga ggaggcctcc cgcgcctcca aggggctcgc
     1441 cctcctcaac tggctgggcg cggcccgctt ctgcggcggc tgcggcgccg agctcgccga
     1501 cgacgggacg gagggctacg cggccggcgc caggcgctgc ccggcctgct cccgcgtcta
     1561 ctacccccgc gtctcgccgg ccgtcatcct cctcgtgcgc cgcgagggga agatcctgct
     1621 cgcgcacaac gcggccttcc ccgcggggcg ctacggcctg atcgcgggct tcgtggagcc
     1681 tggggagagc ctggaggagg ccgcgcgccg cgagctcctg gaggagacgg ggatcgaggt
     1741 cggcgagctc cgctacagcg cctcccagcc ttggcccttc cccgactccc tcatggtcgg
     1801 cttcgaggcg gagtggcgct ccggcgaggg gaagccggac ggcgtcgagc tcaccgagct
     1861 cctgtggcgc ggccccgacg agctgccctc catcccgccg ccgggcagca tagcccgccg
     1921 cctcatcgac ggcttcctca gatccgcagc ttcctcctga gcggccgcct gaacaggcgg
     1981 tagtgcgcgt agaagaggag gcccgaggcg ggaagggccg ctgcggcgac gaagagggac
     2041 cacctgagga cgaagggccc tcccgccgcg agggagagga cggcctccac ggccaggcag
     2101 agcgccgcgg ctccggcggc ggccagggcg aggaggttga ggcccttcct ccgcgaggcg
     2161 gctgcgagca cgacgaccgc cgtcagggct ccgacggagg ccaggtagat cgggaggccg
     2221 tagcggagga accaggcgcc gccgccgtcg gccaggtcga ggaggaggag gagggcgagt
     2281 tcgcctgggc agaggacggc gaagacgagc cagggcttac cccttaggac catgggcatg
     2341 caggcgagga gccagcccat cgcgaggccg acgaggacga cgggcgacca gcccaggcgg
     2401 ccctccgcgt agaggtctat ccccgcgcag agcagggccg cgatcccgaa gacgacgctc
     2461 agggcctcga cggcgagggc gcggccacgg gggccggcgc ttccgccgcg ggcttccgct
     2521 acggcggccc cgctctccgt cgaggcgttc cctccggccg tggccgcccc ggctcccggg
     2581 ccttcggccg gggggctcgg ccggtcgccc gcggcggctt ggacccgcga gaggcagagg
     2641 gggcagacgg tcgccgtccc gtcgagttcg acgccgcatg tctggcagag tggcacgttg
     2701 cttctcctca agatgtggat cgcttcggga agatagacag ggcgggaagg aattcgggtg
     2761 cggtgcgccg cgggccttgg gcgatgctcc gcgccgcggg tctcatgcgg tgcgccgcgc
     2821 cgcggccctt aggcgatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2941 gcgccgcggg cctcaggcgg aacggagtcc gcctagcggg agagggcgcg cctcaagccc
     3001 cgctcgtcga gtatctcgat cctcctgccg cggaccgcga ggaggcccct gtcctccatg
     3061 cgggaaagct ccctcgacag gctcggcctc gcgacgccca gggcggcggc gagccgctcc
     3121 ttggtctcgg gcagggtgac cgagaggagg cccgagccgt cccgctccgc ccgctcgagg
     3181 agccagtcgg cgaggcgttc gccgagagtc gcgaaggagg ccgagcggta cttcgcggcc
     3241 aggaaggcca ggcgcgagcc catgtcgagg agaagggctt cgaggaccgc ggggaagcgc
     3301 gcggccgcgc cgaggaggat gccgcgcggg atcgacgcga gcctggcctc gctccgcgcc
     3361 gacacggaca cgggaaggcg ccgctccggc gcgaagagga cagccgtggc gacggcgtcg
     3421 ggagccttga gcgtctccac gacgacggcg cggccctcgc cgtcggccat ctcggccgag
     3481 gcttcccccg cgaggatgac gcggagctcc tcgtaggccg agcccgcgag gaggatggtg
     3541 cttcccgggg ggtaggaacg gaccgagacc ggcgccgtcc tcaggtagga gccgagctcg
     3601 aggagatcgg cacccgcgaa gagcggcgag gccgcgagga gggcgatgtc gcgatcgtcc
     3661 atgaattcac tgtacccgaa ttgcgcccgg cgcgtgaagg ccgtacaatg ggtggccagg
     3721 aggcatcatg gacttctcgt ggaagatcgt catcgacgcc ggggccatcg ccatggccct
     3781 gcttctcgcc accctcctca gggccaaggt ccgctttttc cagaagtacc tcatcccgaa
     3841 cgcgctcacc gcgggcttca tcctcctccc cgtctacaac ttcctcctcc cctcgctggg
     3901 ctacgcccag caccggctcg gggagctcgt ctaccatctc ctcaacgtgt ccttcatagc
     3961 gatgaccctc cgcgcctctc cgcccaaggt gaaggggcac aagggcggcg gggtcctggg
     4021 catgtcgacc ggaatcctcc tcggctactc gacccaggcc ctcctcggcc tcggcctcac
     4081 cctcctcttc ctgccgagga tcttcccctc cttcggcctc ttcctccccc tgggcttcgc
     4141 cctcgggccc gggcaggcct acgccatcgg caagggctgg gaggcgatgg gcttcgacgg
     4201 cgcgccctcc atcggactgg ccttcgccgc gatcggctat ctctgggcct gcttcggcgg
     4261 cgtcttcctc atcaaccacg ggctcaagaa gggctggctg aaaggggagg gcctggccgc
     4321 gttcaaggac aaggccatga tgtcgggcgt cgtccccctg gacgcggaga agcccgtcgg
     4381 ggcgaggctg tccaccgaca gcgaggccat cgactccttc agcttccacg cggccgcggt
     4441 ggtcttcacc tacctcctct cctacgtctt cctcctcggc ctcacccgcc tcctcgagct
     4501 cgcggggaag cccggccacg acctggccgt caatctctgg gggatcaact tcatcttctc
     4561 ctccatcacc gccatcgccg tgaggaaggt cctggacagg ctccgcctcg ggcgcctcct
     4621 cgacgacggg accctctccc ggatctccgg cttctccgtc gacttcatgg tcgccggctc
     4681 gctcggggcg atctccctca tctttgtcgg gaagttctgg gcggagatcc tcctcatgtc
     4741 gacggtatgc ggcctcatga ccctcgtcgt cgtgccatgg ttctgctccc ggatgttcag
     4801 ggactacgag ttcgagcgca tgctgatgat ctacggcgtg tccacgggca ccctgtccac
     4861 gggcctcgcc ctcctcaggg tgatggaccc cgagttcaag acgagcgtcg cctccgacta
     4921 catgctctcc gccgggctca ccttcgtcct ggccgtgccc ttcatcctct cgatcaacct
     4981 gcccgccaag gccgcgacga cgggatcgat gtcctattac tggatcatga tgggcgtggc
     5041 ggcggcctac ctcgcctacg tcctcgcgag ctacgcctgg atcgcgaggc ggaagagcct
     5101 cgcgaagcca gcctcgcttt ggttcaagga accttgagcg cctcgatgcc catgcaccac
     5161 ttccgcgccg aggtggcgcg gagcgggaag cgcgcctgga agagggtcct gccgacgtag
     5221 aactcgagga ggttgcgctt gtagacgttg gcgccctcga tctcggccag ggggaaggag
     5281 aggcgcgagc cgaagtgggt cgcgagctcc aggcggtcgc ggtagaggag gagggtcccc
     5341 gccctgacgc ggcgcagggg gcgcgtcttc cggccgcgga ggaggagggc gcccggatcc
     5401 gagaggaatg gcgcctccgg ccgctcggcc gcggcgcggg ccgccgccgc cgcgaaggtc
     5461 ttctcctgcc aggcgtccca gtcgcggatt gtcgtgaagg cgggctcccc gcctccgagc
     5521 cgcctgaagc ggtagcggcc gtccagggcc agggcctccc cgcaggccag acagttgaag
     5581 cgggagcgga agctcttgag cttgccgatc gagccgcagg ccgggcacat gaagagcgag
     5641 agctcgaggt gctcggccat ccgccgggac tcgaactcct ggggctcggc ctcgagatag
     5701 gcgcactcgt cgtgtcctat cgccgactcg agccgcgcga ctatctcctc cgccgtgagg
     5761 gccttgagct cggcgggttc gagcgcgagc ttccagtcga cgacgaacct gcccgggcgg
     5821 cgcttccagg tccagcgggg cagggaggag tagcccccgc tgatcctcgc cgcgacgacg
     5881 gggatcttga ggagcttgag gagcttggcc gtggcgggga ggacgggcag gctccggccg
     5941 tcccaggtgg cctgcccctc gggaaacacg ccgacgacgc ccttccgctt ccgcgtcacc
     6001 tccacgatcc agttgatggt ctgcatgtcg ggtatcgcct tggtcttggg gatcgagccc
     6061 acgaagcgca ggatcttgcc gaggaggggc gtccgcatcg ccccgtcccc cgtcacccag
     6121 tagaccggtt ggcggatcag cggcccgagg atgaaggggt cgcgcgtcgt cacgtggttc
     6181 gccaccacga tgaagggcgg cttcagcgcc ttgaggaact cgccgccccg ggactcgacg
     6241 cggtaggcgc gcctgagcca ggctccgaga gtccatttga ggagcttgtt gaaccagcgg
     6301 tagttgcgcg ggacgtagcg gtgcgtggcg ttcatgcggg ccatctcctc tctgccatgg
     6361 agccgatcct agaccgcccg gcgcgaaaag gcaaacggaa ccgctcggga tcgccgccgg
     6421 cgcttgcgcg gcgccttcgc gatggcgctc tcgcggcgct ctcctggcgc ccgactccgc
     6481 tgcgccttag tccttgccga gggtctcgta ggcctcgaga agctcctcgg gtatctccag
     6541 cctcgagcct tcgacggcca caagagtctc gtgtccggcc ggagtgaggg cgaagcggaa
     6601 gcggcgcttg tccttgtcgt cgatgttgcg ctccacgagg cccctctcct cgagggtctt
     6661 gaggatgcgg ctcaggttgg aggcgccgat gccggtcgcg gccgcgatct cggaggggga
     6721 gcggattccg cggcccagag agctcaagac gacggcctcg gcgaggccga ctgcctgggc
     6781 gtccttgagg cgctcatcca ggcggttgag caaggaaact atccgggaga tggattccag
     6841 atcgaccatg gaatattgtg gggatgtact gtcgaagctg tcaatatttc atgtatgaaa
     6901 atattgcgca aatgaagtga agttgtcgcc caagccagtc gagaaaaatt tgatcatcgc
     6961 aaatattgcg cccctgaaat tccgtaaatc gcgcccgcgc gggaagtatt gcgcgaatcc
     7021 ctccttgtgc gcttcgcccc gcgatggcta ctattgccgc atcgaagcag gggggaagcg
     7081 ggcgtggcag agctcaacag accggattcg ggcctcgtgg ccgaggctca tgcgattgcc
     7141 ggccgggcga aggccgagat ggccaagcgc atcgtcggca aggacggagt actaaagggc
     7201 atcctcacgg gcgtcatcgc ctcgggccac gtcctcctcg agggcgtgcc gggcctcgcg
     7261 aagaccctcg ccgtcaagac cttcgcggag atctccggcc tcgacttcag ccggatccag
     7321 ttcacgcccg acctcctgcc cgccgacatc acgggcaccc tggtctacga gcaggcgacg
     7381 ggccgcttca cgccccgccg cggcccccta ttcgccaacg tcgtcctggc cgacgaggtg
     7441 aaccgcgccc cggccaaggt ccagtcggcc ctcctcgagg cgatgcagga ggggcaggtg
     7501 acgataggcg aggcgagcca cgccctgccc gagcccttct tcgtcctcgc gacccagaat
     7561 cccatcgagc acgagggcac ctatcccctt cccgaggccc agctcgaccg cttcctcctc
     7621 aaggtggtcg ccgactaccc gagcgccgag gaggaggcgg ccatcgtgag gcgggtggcc
     7681 gaccgctcct ccgcccccgt ggacagggtc ctgggcgcgg ccgagatagc cctcctcagg
     7741 aaggccgccg agtccgtcgc cgtcgacgag gccatcgtcg gctacatcgt acgcctggtg
     7801 cgcgcgacca ggccgggccc ggagtccccg gcggagctgc gcgacacggc ccgctggctc
     7861 gaatacgggg cctccccccg cgcctcgatc cacctcttcc gctgcgcgcg gatctccgcc
     7921 ctcctcgacg gccgctccca cgtcctcccg ggggacgtga aggcctcagc ccccgaggtg
     7981 ctccgccacc gcctcgtcct ctcctaccag gccgaggccg acggggtcga cgcggacgcg
     8041 gcgatagcgc gcatcctctc ggccgttccc ctcccctagg gcgccgtgga cagggacgag
     8101 ctccacgggc gcatccggcg gctccttttc gtgtcgccgg ccctctcctc ggccttctcg
     8161 cggggagact tccgctccgt cttccgcggc cggggcatgg acttcgaggc cctgcgcgag
     8221 tacggcgagg gcgacgacgc ccgcctcatc gactggaacg tctcggtgcg cttcgcccgc
     8281 cccttcgtcc ggacctaccg ggaagaccgc tccctcacgc tcttcctcct cgtcgacgtc
     8341 tccgcctcca tggaggaggg ctcgggcgag ctctccaagc gcgacgcggc cgtcctctcc
     8401 gcctccctcc tggcctacgc cgcccagctc cggggcatgc ccgtgggcgc cctggtcttc
     8461 gcgggcgggg cgatccgcta cttcgagccc cggcgcggca aggcccacgc gctcgccctc
     8521 atcgaggcgg ccgtcggtgc ccacgcggag ggaagagcct ccgcgggcgg caccgacctg
     8581 ggcggcgcct tcgagcgcgc gggcaggctc ctcaagcgcc gcagcctggt gatggccctg
     8641 agcgacttcg acgcggaggg ctgggccccg tccctcgccc tcctcgcgag gcgccacgac
     8701 gtcgtcgccg tcagggtcac ggacgggctc gaccgcgggc tgcccgccta cggggccttc
     8761 ggggcggccg acgccgaggg ccggggcccg gcctggctgc cgctcgggag ccggtccttc
     8821 cgggcgcgtt gggcgaaacg gggagaggcg aggcgcgagg cctgccgcgc gctctgcgcc
     8881 gaggcggggg cccgcctcct ggagatcgac acctccgacg atccggcgcg gcgcctcctc
     8941 gagttcttcg accggaggag gcggccttga gccggcgcgc cagcgaccac ggcgtcggcg
     9001 ccggacgcgc aggcgccggg cgcggctcct ccgcgctcca gcgggccgcc cgccgcggcg
     9061 agccgtccgg gcacgccgcc ggtggggagc agcccaggcg cgccgcccgc agcggggagc
     9121 cgtcccggcg gcccgcgccg cggggcctcg ccgcggccat cctcgccctg tgcctctccg
     9181 ccctgcccgc gggggccgag ccgctcggcc tggttcgcct gggcttcgcg ccggcccgct
     9241 tcggcgcggg cgaggaggtc gaggccgtcg ccctcctcga ggcggggagc gccgagcccg
     9301 aggccttcgc cctcaaggcc ggcggcggtc tccccgagcc cgggcccggc gcggatcccg
     9361 agctccgcga gctcagactc tcgaagacgg ccggaggctg ggagctccgc gcgcgcttcg
     9421 tgccctggac gccggggaag ggcctcctgc ctccgctctc cgtccgcggc ctcgccctgc
     9481 cggggctgcc ctacacggtc gactccgtcc tcgccccggg cgaccgcgag ccggccccgc
     9541 ccaagcccca gcgggacccg cccggcacgg ccctctacct ctacggcttc gcgggcctgg
     9601 tcctggtcct cgtcctcgcg ggcttctggg ccgccgccgt cctcgtgccc ggcgcgcggc
     9661 gcctcctcgc gcggcgacgc gcggcccagg ccaggcgcgc cttggagagg agcctcgcct
     9721 acctggagcg cggcctgggc ggctcctcgc ccgcggactt ctacgcggcc ctggcgcggt
     9781 ccctgcggct ctacctcgcg gcccgcctcc tgcccgaggc cgccgccctc acgcccgcgg
     9841 agatcggggc cctgcccgcg gcccgcttcc cggaggccgg catcagggac gacgcggcgg
     9901 ccgtcctcgg ggaggccgac ctcgcccgct tcgcgggcct gaacccgggc ctggacgcga
     9961 tgcgcgccgc cctggagcgc tccgcccgcc tcgcgggctc ggcggaggag gccctcgatg
    10021 ctctcctttg agcggccggc cctcctcgcc ttcctcctcc tcgtgcccct cctcgccttc
    10081 gcgcggagac tcttccgcgc gcggctctcg gccccgcgct tccccgtcgc cgccctgccg
    10141 ggagaggccc cgccggctcc gcccctcgcg cggagggccc tcttctttct cgcgcaggcc
    10201 ctcctctggc tcggcctcgc cctcctcgtc ctggcctcgg cggggccggc cagggtgagc
    10261 cgccgcgtcc tctacctctc gcggggcaac gagatcgtct tcgtcctgga cgtctcgccg
    10321 agcatggccg ccgaggactt cgagccgacc cgcctcgccg cgtccaaggc catcatcgac
    10381 gagttcctct cctcgcggcg gaatgagacg gtgggcctcg tcgccttcgg cggggaggcg
    10441 gccctcgtct gcccgccgac cctggactac ggagtcctcc ggaggcgcct cggggatctc
    10501 aagcccggcc tcctgggcga gggcacggcc ctgggcgcgg ggatcggcgt cgcgctggcc
    10561 cattcgggcg gcaagggcgc gccggcgcgg tatgtgatcc tgctcacgga cggcgagaac
    10621 aacgcggggc ccatggctcc ggcgaccgcc gccgccctcg cggcccgccg cggcgtctcg
    10681 gtctccgtca tcggcgtcgg gagccggggc gaggtgcccg tcacctacgt cgacccggac
    10741 acgggccaga ggcggagcgg cacctacttg agcggcttcg accgggcctc cctcgagtcc
    10801 ctggcgagga taggcggcgg cagctacttc gccgcggaga gccccgaggc cctcgagcgc
    10861 gccttctccg ccgtctcgga ggactcggtc tccctggcga tgacaaggtc ggtctccacg
    10921 gaggactccc tcgcgaggcc cctcctcgcc ctcgcgttgg ccgccctcgt cctcgcgcgg
    10981 ctcctcggcc tcctttcggg gggggatttc ctatgagctt cgagcgtccc gagctcctgt
    11041 ggctcctcct cctcgcgccc ctcgagctcc tcctcgcgct gcgcagggcc ccgaggctcc
    11101 gcgcctctct cgagctcctc tcgggtcccc ggcgccgcga ggcccagggc gcccgctacg
    11161 ccgcggcctc ggtctacggg acggcggctt ccgtcgtcct cgccgccgcc tcggccctgg
    11221 ccctcgcggg gcccgcctgg ggcgtgaagg ccgtcgcttc ggagcgctcg ggcctggagg
    11281 tggccgtcgt cctggacgtc tcgaggagca tggaggtccg cgagggcgga gcctcgcgcc
    11341 tcgaaggcgc caagtccctc ctccgcggcc tcctcaggca ggcaccgggg gcctccttct
    11401 ccctcgtcgc cgccaagggc gacgccgtcc tcctcgtccc gatgacggag gacctcgagg
    11461 ccctggactc ggccctggac tacgccgacc ccgagacgac gacggcggcg ggaaccgacc
    11521 tcgggaaggg catcagggcg ggcctcgaga gcttcaccgc gggctccggc gccagccgcg
    11581 ccggcgcgga ccgagccgga gtgaaccgcg ccctccttct cctctccgac ggcggcgaca
    11641 aggggggcga ggccctcaag gcggccgagg aggcgaggaa gcgcaagacg aggctcctcg
    11701 cagtcggcct gggcggcgcc gaggccctgc ccgtgcccgg cccctcgggc ggccccctcc
    11761 tggacgcgaa gggcgacgcc gtgaggagcg ccctggagcc gaccctcctc cgcgccgtcg
    11821 ccgcggcctc gggcgggcgc tacctcgagg cctcggactc gggagcggcc caggccctcc
    11881 gcgccgagct cgccgagctc ggccgcggcg ggaagcgcgt cgagtacgaa tcgaaggaca
    11941 ggagctcctt tttcatcctc ctcgccctcg cggccctcgt cgtccgcgtc gtcgcctcca
    12001 tcctggcctt cgccgctgcg gatcggtcga ccgtcgaggc agggcgcgcc gccccaggca
    12061 ggcccgccgc cgccgacagt cccgccgcgg aaaggacagg ccgatgagaa ggcggctggc
    12121 tctcctcgcg gccgccgcgg ccctcctcgc gacgggctgc ggcgccctgc gcgactacga
    12181 cgcggtgatc aaggggaacc gcctccacgg caggggccgc taccaggagg cggcggcctc
    12241 ctacctgtcg gcggggaagg gagccttcgg cgccgtcctc tcctacgacc tcgcgaacgt
    12301 ctacgcccgc ctgggcgagg tccaggccgc cgccgagctc tacgccgagg cgcgcaaggc
    12361 gggcgacgcc tccttgagcg cggcggcctt ctacaacgag ggcctggccc tctacgagaa
    12421 gggccgctac gaggaggcct accgcgcctt ccgctcggcc ctctccctcg acccccgcga
    12481 cggggaggcg aggcgcaacc tcgagatagc ctggcgcgac tggaagaaga gcgccctctc
    12541 gccgccagag cgggcggcgc cctcctcgag gagccaaggc gagggcgacg aggagctcag
    12601 gctgctccgc cgcctggaga ccgggcgcta caagccggga gggacgacgc cccagctccc
    12661 gaaccccgag gactactagc atgagaagcg caagactgaa ggcgcccctc ctgggcctcg
    12721 ccgtccttgt aggcctcggc gcggccggac cttcccgcgc cgcccccgcg tcgagcgcgc
    12781 ccgcggcggc cgcggcggcc ccggccttcg tctaccgcct ctccgtcgag cccgagagcc
    12841 cgatggcggg cgagcccttc gccgtcgtcc tcagggtcga ggggctgacg cgcgtctccc
    12901 tggacaagct cgtcctcgac cccctcctct ccctcgaggg gagctcctcg cggccccgct
    12961 tcgagctgtc cacgggcatg cgcgagacgg agacccgcct ggagctcaag gccctcgccc
    13021 cgggctccct cctcatcgac tccatcgagc ttgcggggct cgagggccgc ctcgccgtcg
    13081 gtcccctgcg gatagaggtc cgcggcgccg cgaaggccga ggccggcccc ctctggtcct
    13141 gggaggcgcc cgattccgcc tatcgctact ccaccttcgg cctgcgcctg gctggcccgg
    13201 ccggcgcggc cgaggacgcc ttcgcctcct tcgacgcgcc gccgggcctc gccctcgagc
    13261 cggcggggga cggcctctcc tggatctgca cggccctgga ggaggggcga ctccgcctcc
    13321 ccgaggcctc ggtcgaggcg ggacccgact cggggaaggc ccaggcgcgg agcatcctcg
    13381 tgaggcccct gccgcccggg gcggccgcga cgcgggccgt cggcgacttc gagctgcgct
    13441 tcgaggggcc cccttcgggc aaggggaggg caggcgaggc cctgggcttc aggctgacgc
    13501 tctctggctc ggggaaccat ccggccctgc gattccccgg cgtcgcggcc agcctggacg
    13561 ggaagcccct gccggagggc agcctctcgc caaggcggag cgaggcgctc aagcagggcc
    13621 cgcggggcta cgagggcagc tccgtcctcg agctcgacct agtccctccc gccgccggcc
    13681 tcctccgcgt ctcgccctct cccttcgcct acgtcacgcc cgagggccgg agcctggagc
    13741 tcagggtgga tccgatcgag gtccgcgtcg cgcccgcggc gccgggcggc gccgaggagg
    13801 ggcggggcga tcccttcgcc gaagccgtcg ccgcgctgga ggccctgccc gaaggcgccc
    13861 gcggcgcggc cggcgccgcg ctcatcgccg ccgccgcttc ctggaaggcc ggcgagaggg
    13921 ggaaggccct ggcccaggcc tacggcgcgg cgcggcgctt ccccttcgac gcgcgcgcgg
    13981 cgaaggccgc cgacgccgcc tccctcgccg tcggggccgg gcccaggcag cgcgacgcct
    14041 atgccccgcc cgccttcttc gcctggattg gctcggggct cttcgcgctg ggagcgggcc
    14101 tggccttggc cgggcggccg aggcgccgcg gcttaggccg cccggggacc ggcggcgcga
    14161 aggccggcgc ggaaggccgg cgcgcggagc aaggcggcgc gaaggccggc tcaggcggac
    14221 ggaaggccgg aaggggcgca aaggcggccg acgcgcgtcg gcgggccaag gcggcgcgcg
    14281 gcggcccggg cggacggccc gtcctgggcg cggccggcgg cctcctcctc gcgacggccc
    14341 tcgcggcctt cgtcctggcg ggcctggccc tgcgggaacg ccgcgtcgag tacgccgtcg
    14401 cctggacgga cagggcctat cccgtcccct cggccaaggc ctcgggcacc gtcctcgtgg
    14461 acaggggagc ctcgggcagg gtccttggcc gcgcccccgg ctttgtcggc ctgcgcctcg
    14521 cggacggcag cgtggcttgg gtgccagccg aagccgtata cttgtactga ggtgaagcat
    14581 ggatttcggg gacattctgg acgagtggga caagcgcagc tccaagcccg gcggcctcaa
    14641 gggcgcggac gaggtggagc gccgcctccg cgaggcggag gcctcccgcc tcgccctcct
    14701 caaggaggag gagcgcagga aggccgcggc caaggccagg gcccgctaca gcctggagag
    14761 ctggctcgac gagcacggcg tggaggacaa ggacgggaag gacgagcccc aggcgggcgg
    14821 gcaggcctcg cgggaggccg aggcgcggcg cctaaaggac ctgaagcccc aggccaggct
    14881 ggacctccac ggcatgcgcg cgctggaggc cgaggcggcc ctcgcctcct tcctggaggc
    14941 ctcgtccagg gccggcctcg agaaggtgct cgtcgtgacg gggaagggca tgcactcgca
    15001 gggggagccc gtgctgggaa agacggcgcg gcgcgtgctc gagtcgagcc ccttcgccgg
    15061 ccgcttcggc caggcagacc cgtcggaagg cggctcaggc gccctctggg tcatcgtgcg
    15121 taggaaaggc tatttttcgc gatagatgat gcgtccgcgg ttgaggtcgt agggggagag
    15181 ggcgactttc accttgtcgc ccgggacgat gcggatgtag tgcttcctca tcttgccgga
    15241 gaggtgggcg aggatcacgt gcccgttggg gagctccacg cgaaacatgg tgttcggtag
    15301 ggactccttg acgatgccct ctacttcgat ggcttcttct ttagccacgg ttcctccttg
    15361 atggaatggg cctccgcctt ggggtggcgg actctcgagc tgcgcgagga atccggcgcc
    15421 ggaatttgct ttgcgtacgg aagcgcgtat aatagtacgg gtcggggctt ggaatgtcaa
    15481 tcggccttga ctttttcggg catctcgtgc atggtgccgg tcccctctcg ggaccactac
    15541 cagaggggcc gatcgacatg aggaggtagc gaatggacaa ggacttcatc gagcggatgc
    15601 gctcatcgct cgtcgctcag aagaaggaga tcctcgacac gctcgccgct acgaacgccg
    15661 atttccgggc catcgtcgag gagatggacc cgaaggactt cgccgacgtc gcgagcgacg
    15721 acatcgaccg caagatgatc gaggctctcg gcagccagga cataaagcgc gtccgcgcga
    15781 tcgacgcggc cctctcgcgg atccagcagg gccactacgg cctctgcatg aagtgcggaa
    15841 agaagattcc gatagaccgg ctcaccgcaa tcccctacgc cgtcctctgc atcgagtgcc
    15901 agaagggcga ggagaggcgc aaccgctagg atcccgggcg ggcgcggccc gctcccgggc
    15961 catggggccg gcgccgcggg cgccggcctt tccttttccg tgccatggcg ggggaagccg
    16021 gcgctaggtc tcgtcgatcc cgacctcgat ccagctgccc ttcgtgagga cggccacgct
    16081 ttcgtagccc gcggccctcg cgagctcccg cgccgcgtcc aggtggacgc cgacgtgcct
    16141 cgtgtcgtgg gcgtcggcgc agaaggtgat ggggacgcga agccgcttga gctcgcgcag
    16201 gatgggaagg gaggggtagg gctccttcgt cttcccgcgg gccaggccgc ccaggttcag
    16261 ctccgccacg atgcctttcc cctcgaggag gccggccgcc tcgaaggccg cggccaggta
    16321 ggccgggtcc tcctcgtcga agagccggcc gcccgggttg t
//
LOCUS       DAOOSJ010000024        15811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2526_length_15811_cov_6.122937, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000024 DAOOSJ010000000
VERSION     DAOOSJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_15811_cov_6.122937"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..458)
                     /locus_tag="PLG14_02415"
     CDS             complement(144..458)
                     /locus_tag="PLG14_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOX48014.1"
                     /translation="MSRVLLSVLVMALVTLFTRAAPFLFFSRRRPPAFLDYLQRYIPP
                     VVMSLLVLASFKDIDFAAAPHGLPALAGAALTALLHGWKRNVLLSIGGGTALYMLLIR
                     LV"
     gene            complement(455..1252)
                     /locus_tag="PLG14_02420"
     CDS             complement(455..1252)
                     /locus_tag="PLG14_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOX48015.1"
                     /translation="MSGKQAQAAGGLLSAAFRASLPVLLGYTTIGLAFGLVLVGSGLP
                     WWLSPLMAVFVYAGAAQFMGVGLLAAGTGPAEIGLLTLLMNARHAVYGLSLLERFRKA
                     GAVKPYLVFGLTDETYGLLTTVAPPEGADGIRFCALVTALDQAYWVAGCAAGALLGRA
                     LPFDTSGLDFALTALFVVLLVEQLRSVRRAEPYLVAVLAAAAARLLASPRDFLLVSLA
                     LAIGGLALLRGRLEGPAGGSGRKPGSGGPGARTEAPAAEAGPEGAAR"
     gene            1396..1800
                     /locus_tag="PLG14_02425"
     CDS             1396..1800
                     /locus_tag="PLG14_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48016.1"
                     /translation="MAKRPAFALDSLFFLQLSLGLFFLALGVMGLADYNSKLSEVARF
                     FGRNDGLRIVVSLVELVMGGLLVLGLFLSVSSGLAKIFSLALFGLWAVYILYYFFFNN
                     LFEPDFLPWLQEVAWRSIVLCSLWIVGRKYMA"
     gene            complement(1986..3653)
                     /locus_tag="PLG14_02430"
     CDS             complement(1986..3653)
                     /locus_tag="PLG14_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48017.1"
                     /translation="MSKRLLIRDLTLRDGQQSQFATRMNQAQVDRVLPLYKNAGFYAM
                     EVWGGAVPDSVMRYLDESPWERLEKIKAGVGGASKLTALSRGRNLFGYTPYPDSVIEG
                     FCKNAVRSGVDIMRIFDALNDVDNMTSSMRFVKEAGGMADCAVCYTVDPHFPRKERFQ
                     AFLKGRKLPKDLFTIRYFVDKAKELERLGADMITIKDMAGLVDPEESARLVKALKAEV
                     GIPIDLHTHCTPGYGTASVLMAMVHGVDVVDTTALAWSGGPAHVAYEIVQLFADRLGL
                     ETGVDRAAVGAMDREFRLIRAELAKFDQYPDNNPPVIDLSRHKLPAEDTALFDDAVEE
                     ALRGRTDKVLELCHRIEARFNYPAPDEAVRIAQIPGGMYTNMLAQLEEAKLGDKLAEV
                     LEAVPDVRLACGVPPLVTPTSQIVGVQAVNCVVDRAQGKPFYTNVSKNFAELVKGSYG
                     KTPWPVDPAFRERICGVREETPYDTSKYKKQPNPELADCGGSKLALNEKEELLLELFP
                     SVADKFLRGQRKREWLQANPPPPPEPEKPQRYPALFWEEELPLGMEA"
     gene            complement(3779..4510)
                     /locus_tag="PLG14_02435"
     CDS             complement(3779..4510)
                     /locus_tag="PLG14_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOX48018.1"
                     /translation="MPEKDNFVRAVDRALDILDCFAPGRLELSLTEISKRIDLAMSTT
                     SRIVASLERRGYLSRSAENQRYSLGPKIAQLGALGFSNVDIRKVALPYMRELNAIYDE
                     GVSLYVVQGDERVCVERVESSRPLRRVINVGDRHRLTRGAAGRVLLAYLPEEKRKALL
                     AKDPFTTEAGLAELRRNGYTVSLGEREEGVTSIAAPVLDARREIVAALAMSGPSVRFE
                     GPGFDDKIAKVRGHADLISAALGRL"
     gene            4956..5231
                     /locus_tag="PLG14_02440"
     CDS             4956..5231
                     /locus_tag="PLG14_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX48019.1"
                     /translation="MSEQKKVFFHVVREKWCKGCNICVAFCPKQVLELKGGKVAVARP
                     EDCIGCRMCELRCPDYAIEVHEKGPEPAPGKDAASQDYDLPPEADHA"
     gene            5224..6357
                     /locus_tag="PLG14_02445"
     CDS             5224..6357
                     /locus_tag="PLG14_02445"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOX48020.1"
                     /translation="MPKTIKLMQGNEACTAGALAAGLRFYAGYPITPSTEIAELCSEE
                     LPKIGGKFVQMEDEIGSIAAIIGASLAGKKAMTATSGPGFSLMQECLGYASLCEVPIV
                     IVNVQRMGPSTGMPTSPAQGEVMQSRWGTHGDHPVIVLAPGNVRECFDLTLRAFDLSE
                     KYRVPAIVLTDEVIGHMREKVELPDPADVKPLRRAAPAGRTGYKPYAADPNGGVPLMA
                     SFGEGYRYHVTGLFHDETGAPSSKTEVADRLVRRLQAKVDLHADEICDYASESLEDCE
                     VAVISFGSSAMSSLSAVRRARKEGVKAGMLRLKTIWPFPEKAVRELAGHAKTILVPEM
                     NLGQLALEVERVVAGRARVVRVGKVNGELFRPEEIHSAVMGAK"
     gene            6357..7217
                     /locus_tag="PLG14_02450"
     CDS             6357..7217
                     /locus_tag="PLG14_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOX48021.1"
                     /translation="MSEMLSYFRQERLPHIWCPGCGHGTITGALVRAIERLGLDKNKV
                     CVVSGIGCSSRAVGYLDFDTLHTAHGRALAFATGIKLARPDLKVIVMTGDGDCTAIGG
                     NHFIHAARRNIGITTIVMNNEIYGMTSGQYSPMTPKGFIGTTAPYGNLEPHFDLCELA
                     KASGASYVARATSFHVPLLIDLIEKALRNDGFSVVEAIAQCPTYLGRRNKLGSAVDML
                     NWIKDQTINAKAAAALPPEKREGKLLIGELHNAPAPEYTASYGELITKVQRSFENVKG
                     GLWECAPNSD"
     gene            7196..7732
                     /locus_tag="PLG14_02455"
     CDS             7196..7732
                     /locus_tag="PLG14_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOX48022.1"
                     /translation="MRTEFRLSGSGGQGLLLAGIVLAEAAILEDKNAVQTQSYGPEAR
                     GGSSKAEVVVSDEDIDYPKATDPDYLLALTADSYRTYGKLQGKGLIIVDSSVALDPEI
                     KARTVSLPILDTAATKVGKKVVANIVALGVLGGISGVVSPAILEKAVLNRVPKGTEEL
                     NLKALRAGLELAAGAKKA"
     gene            7754..9421
                     /locus_tag="PLG14_02460"
     CDS             7754..9421
                     /locus_tag="PLG14_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOX48023.1"
                     /translation="MSDNEKLKKGREAYDAGVEKGLSKFPERRKEFVTGSGSPVGRLY
                     TPLDLEGRDYLADQGFPGEYPFTRGVQNTLYRGRFWTMRQYAGFSNAEESNKRYKFLL
                     EQGTTGLSIAFDLPTQIGYDSDHPLSQGEVGKVGVAIDSLADMEILFDGIPLDKVSTS
                     MTINAPASVLLAMYIAVAEKQGVTPDKLNGTIQNDILKEYVARGTYIYPPSPSMRLIT
                     NIFEYCSKEVPNWNTISISGYHIREAGSTASQEVAFTIADGIAYVEAAVKAGLDVDDF
                     APRLSFFFNAHNDLFEEVAKYRAARRVWAKIMKERFGAKSPKSWMLRFHTQTGGSTLT
                     AQQPDNNVVRVAIQALAAVLGGTQSLHTNSRDEALALPTEESVRIALRTQQIIAYESG
                     AAETVDPLAGSYYVESLTNRIEDEALAYLKRIDELGGAVKAIEQGYVQQEIQDSAYRY
                     QMDIEKGDRVVVGLNKFQVKEKAPEGLLRVDPSVGTRQCEKLAALKAKRDNAKVKAAL
                     AVLETAAKGDANLMPPILEAVRAYATLGEICDVMRGVFGEYRPSVMF"
     gene            9434..9832
                     /locus_tag="PLG14_02465"
     CDS             9434..9832
                     /locus_tag="PLG14_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOX48024.1"
                     /translation="MERKIRVLVAKPGLDGHDRGAKVIARALRDDGMEVIYTGLRQTP
                     EQIAQAAVQEDADVVALSILSGAHNHLFPRVIELLKEKGADDVLVIGGGVIPEADYDS
                     LKSKGIAAIFGPGTPTKATIDFIRENLKRK"
     gene            9843..11024
                     /gene="meaB"
                     /locus_tag="PLG14_02470"
     CDS             9843..11024
                     /gene="meaB"
                     /locus_tag="PLG14_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOX48025.1"
                     /translation="MNTPKAGGAASARPLAERVLAGEQRAVARLISGLEDGDPAAVGE
                     MRSLRGRAGRAHLIGITGPPGSGKSTLTDKLIGVLRARGARVGVLAVDPSSPFTGGAI
                     LGDRLRMQGHATDEGVFIRSLASRGRLGGLSRATQAAARVLDAAGYDYVVIETVGVGQ
                     SEVDIVKIADTVVLVSVPGLGDDIQVIKAGIMEIGDVFVVNKSDREGSDRVVREIRGM
                     LETAAALKRAALPEPVDTLSGRGAAGLGAAGLGAATGPGLPGAGQGRQAPAPLSGEAL
                     SHHLTESANSVKLADDSGLPPVLKTVAETGEGVKELVDAILAHRAGLAASGALEARRL
                     EGIRAEMRALIAFRLLDALEGGAGRAREDALSRAVLGGDADVYGASEELFSSIAKGEA
                     K"
     gene            11021..11425
                     /gene="mce"
                     /locus_tag="PLG14_02475"
     CDS             11021..11425
                     /gene="mce"
                     /locus_tag="PLG14_02475"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOX48026.1"
                     /translation="MKVLKVDHIGVAVEKLDEALAFYTGVLGLELHGTETVEEQKVRT
                     AFLPIGGTEVELLESTDPEGPIGKFIAAKGAGMQHIAYRVDDIEAALAELKAKGVRLI
                     DEKPRYGAGGARIAFLHPKATGGVLVELCERK"
     gene            11467..13011
                     /locus_tag="PLG14_02480"
     CDS             11467..13011
                     /locus_tag="PLG14_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOX48027.1"
                     /translation="MDDKIKELAERRRKIEEGGGPKRVAAQHEKGKMTARERILSLLD
                     SGSFTEIDAFVEHRCTDLGMAEVEAPGEGVVTGYGTVDGRLVYVFAQDFTVIGGSLGA
                     MHAAKICKAMDMAVKVGAPVIGINDSGGARIQEGVDALCGYGDIFFRNVKASGVVPQI
                     SVIMGPCAGGAVYSPALTDFVFMTEKTSNMFITGPQVIKAVTGEDVTAEALGGAHVHA
                     ATSGVAHFAYPDEAQTIAGIKKLLSFLPSNNIEDAPLAPCADDVSRIDEALEEIIPES
                     PNKPYDVRDVIARIFDSGDYLEVQPIYAQNIVTCFARLGGRSVGIIANQPKSMAGVLD
                     INASDKAAHFIRFCDSFNIPLVTLVDTGGYLPGVGQEHGGVIRHGAKILYAYSEATVP
                     KITVILRKAYGGAYIAMCSRHLGADQVFAWPTAEIAVMGPDGAANIIFKKEIDESSDP
                     AATRKEKIAEYKKNFANPYKAAVRGYVDDVIDPEETRPRLARALSMLLGKRESGPARK
                     HGNIPV"
     gene            13023..13376
                     /locus_tag="PLG14_02485"
     CDS             13023..13376
                     /locus_tag="PLG14_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOX48028.1"
                     /translation="MDQWSLTALGMGTVFLTLIFLALIVSLFPLFFRKPEKKRKDPAP
                     LPSLSSQAAPAAAPSGAAGPELVAVIAAAVAAASGSAPGSFRIASIARSESSGGFNTP
                     AWGHADRLGRTSFNS"
     gene            13390..13773
                     /locus_tag="PLG14_02490"
     CDS             13390..13773
                     /locus_tag="PLG14_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOX48029.1"
                     /translation="MKKYRVTVNGQSYDVGVEELGAAPSFAAPAAAAAPVPAAAPAAP
                     AAPAPSAGPAVAGATTVKAPMPGTVMSFKAIVGQHVKRGDVLLILEAMKMENEIVAPA
                     DGTVASIRVPQGAAVNTGDALVDLS"
     gene            13783..14913
                     /locus_tag="PLG14_02495"
     CDS             13783..14913
                     /locus_tag="PLG14_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOX48030.1"
                     /translation="MWEGILSFLQSTGVVSITSGQILMLAVSCVLLYLGIYRKYEPLL
                     LVPIAFGMLLANLPISGITDGPANGQPGGLIYYLYQGVKFGIYPPLIFLGVGAMTDFA
                     PLIANPSSLLLGAAAQFGIFIPFVIANYIGFDMKSAACIGIIGGADGPTAIFLTSRLN
                     PDMLGPIAIAAYSYMALVPIIQPPIMRALTTKKERAVVMGQLRDVSKTEKVVFPIAVT
                     IIIGLILPPAVPLVGSLMLGNLFRVTGVTERLSDTAQHALINILTIFLGVAVGSTASA
                     ERFLNPQTLKIIALGLFAFASGSAMGVIFGKIMYLVTGGKVNPLIGSAGVSAVPMAAR
                     VSQVVGQEENPGNYLLMHAMGPNVAGVIGSAVAAGVLLSVLG"
BASE COUNT         2558 a         5638 c         5277 g         2338 t
ORIGIN      
        1 accccggccc ggaggccgag cgcaggatgc gcgaggccgg agggcaagtc ctctttgccg
       61 tttcgtctgg cgccgaggcc gttccggcat gcgaaccctc ccgcctccgt ctcgtcgccg
      121 ccctccagtc ccgccgcgat ttcctagacc agcctgatga gcagcatgta gagcgccgtg
      181 cctccgccta tggagaggag gacgttcctc ttccagccgt ggaggagggc ggtgagggcc
      241 gcgccggcca gggcgggaag gccgtggggc gccgcggcga aatcgatgtc cttgaaggag
      301 gcgagcacga ggaggctcat cacgaccggc gggatgtagc gctggaggta gtcgaggaag
      361 gccggcggcc tccgcctgga gaagaagagg aagggcgcgg cgcgggtgaa cagcgtgacc
      421 agggccatga cgaggacgga gaggaggacg cggctcatcg ggcggctcct tccgggccgg
      481 cctcggcggc aggggcttcg gtccgcgccc ccggcccgcc gcttccgggc ttccggcctg
      541 agcctcccgc gggcccctcg agcctccccc tgaggagggc gaggccgcct atcgcgaggg
      601 ccagggagac gaggaggaag tcccggggcg aggcgaggag gcgggccgcc gcggccgcca
      661 ggacggcgac gaggtagggc tcggcccgcc tgacggagcg cagctgctcg acgaggagga
      721 cgacgaagag ggccgtgagg gcgaagtcga ggccgctcgt gtcgaagggg agggcccggc
      781 ccaggagggc tcccgcggcg cagcccgcga cccagtaggc ctggtccagg gcggtgacga
      841 gggcgcagaa gcggatcccg tccgcgccct cgggcggagc caccgtcgtg agaaggccgt
      901 aggtctcgtc cgtgaggccg aagacgaggt agggcttcac ggcccccgcc ttcctgaagc
      961 gctcgaggag ggagaggccg tagacggcgt gcctcgcgtt catgaggagg gtgaggaggc
     1021 cgatctccgc ggggccggtt cccgcggcca ggaggccgac gcccatgaac tgggccgccc
     1081 ccgcgtagac gaagaccgcc atcaggggag aaagccacca gggaaggccc gagcccacga
     1141 ggacgaggcc gaaggccagg cctatcgtcg tgtagccgag gaggacggga aggctcgcgc
     1201 ggaaggccgc ggagaggagc cctcccgcgg cctgtgcttg cttgccgctc atggcccgat
     1261 ggtacgcggc ctggccccgc tcggacaagc gcccgtcgcc ggcccgccgc cccgggccgg
     1321 agggctcggc ttgacgccgc ccggggcggc cgctaggctt gacccatcgc gcggcgcgcg
     1381 aaaggagctg cttccatggc aaagcgaccg gccttcgccc tcgattccct gttcttcctc
     1441 cagctgagcc tcggcctgtt cttcctcgcc ctcggcgtca tggggctcgc cgactacaac
     1501 tcgaagctct ccgaagtcgc ccgcttcttc gggcggaacg acggcctgcg catcgtcgtc
     1561 tccctcgtcg agctcgtgat gggcggcctc ctggtcctcg gcctcttcct ctccgtctcc
     1621 tcgggcctcg ccaagatctt ctccctggcc ctcttcggcc tctgggcggt ctacatcctt
     1681 tattacttct tcttcaacaa cctcttcgag cccgatttcc ttccctggct ccaggaggtg
     1741 gcgtggcgct ccatcgtgct ctgctccctc tggatcgtgg gcaggaagta catggcctga
     1801 ggcgaagccg gcggcggccc gccgcgtcca tcgccggttc ggctcggcct ttcgacggcc
     1861 cgccgcgtcc gcgcccggac cccggtcggc tggccgcggt caccaaaaga aagggccgcc
     1921 cggaatcgct cccgggcggc cttgcctttt tcgcgggccc tgtccgttcg gcggcccttg
     1981 ggcctctagg cttccatccc gagggggagc tcctcctccc agaagagggc ggggtagcgc
     2041 tggggcttct cgggctcggg cggcgggggc gggttcgcct ggagccactc gcgcttccgc
     2101 tggccgcgga ggaacttgtc ggcgacgctc gggaagagct cgaggaggag ctcctccttc
     2161 tcgttcagcg cgagcttcga gccgccgcag tcggcgagct cggggttggg ctgcttcttg
     2221 tacttcgagg tgtcgtaggg cgtctcctcg cggacgccgc agatcctctc gcggaaggcg
     2281 gggtcgacgg gccagggggt cttgccgtag gagcccttca cgagctcggc gaagttcttg
     2341 gagacgttcg tgtagaaggg cttgccttgg gcccggtcca cgacgcagtt gacggcctgg
     2401 acgccgacga tctggctcgt cggggtgacg aggggcggca cgccgcaggc caggcgcacg
     2461 tcgggcacgg cctcgagcac ctccgcgagc ttgtcgccga gcttcgcctc ctccagctgg
     2521 gcgagcatgt tcgtgtacat gccgccgggg atctgggcga tccggacggc ctcgtcgggg
     2581 gcggggtagt tgaagcgcgc ctcgatgcgg tggcagagct cgaggacctt gtcggtcctg
     2641 ccgcgcaggg cctcctccac ggcgtcgtcg aagagggcgg tgtcctccgc ggggagcttg
     2701 tggcgcgaga ggtcgatgac gggcgggttg ttgtcggggt actggtcgaa cttggcgagc
     2761 tcggcgcgga tcaggcggaa ctcgcggtcc atcgcgccga cggcggcgcg gtccacgccg
     2821 gtctccaggc ccaggcggtc ggcgaagagc tggacgatct cgtaggcgac gtgggcgggg
     2881 ccgcccgacc aggccagggc cgtggtgtcc acgacgtcga cgccgtggac catcgccatg
     2941 aggacgctcg cggtgccgta gccgggcgtg cagtgggtgt gcaggtcgat ggggatgccg
     3001 acctcggcct tgagggcctt cacgagcctg gccgactcct cggggtcgac gaggccggcc
     3061 atgtccttga tcgtgatcat gtcggcgccc aggcgctcga gctccttcgc cttgtcgacg
     3121 aagtagcgga tcgtgaagag gtccttcggg agcttccggc ccttgagaaa ggcctggaag
     3181 cgctccttcc gggggaagtg cgggtccacg gtgtagcaga ccgcgcagtc cgccatgccg
     3241 cccgcctcct tcacgaagcg catggagctc gtcatgttgt cgacgtcgtt gagggcgtcg
     3301 aagatgcgca tgatgtcgac gccggagcgg acggcgttct tgcagaagcc ctcgatcacc
     3361 gagtcggggt agggcgtgta gccgaagagg ttgcggccgc gggacagggc ggtgagcttg
     3421 gaggcgccgc cgacgccggc cttgatcttc tccaggcgct cccaggggct ctcgtccagg
     3481 tagcgcatga cggagtcggg gacggcgccg ccccagacct ccatcgcgta gaagccggcg
     3541 ttcttgtaga gggggagcac gcggtcgacc tgggcttggt tcatgcgggt cgcgaactgc
     3601 gactgctggc cgtcgcggag ggtgaggtcg cggatgagga gtcgtttgga catcgtattc
     3661 tccgtcgagc gtgatggccc cgtcgcggtt tcctcccgcc ggggcggttc gccctccagt
     3721 atagccgcgc ttcgcggaaa gcggcaatcc gtgccgcgcg gcgccgcggc cgccggccct
     3781 agagccggcc cagggcggcg gagatcaggt cggcgtggcc ccgcaccttg gcgatcttgt
     3841 cgtcgaagcc cgggccctcg aagcggaccg aggggcccga catggccagg gccgcgacga
     3901 tctcccgcct cgcgtccagc accggcgcgg cgatggaggt gacgccctcc tcgcgctccc
     3961 ccaggctcac cgtgtagccg ttccgcctga gctccgccag gcccgcctcc gtcgtgaagg
     4021 gatccttggc gaggagggcc ttgcgcttct cctcggggag gtaggcgagg aggacgcggc
     4081 cggccgcgcc gcgggtcagg cggtggcggt cgccgacgtt gatgacccgc ctcagggggc
     4141 gcgagctctc cacccgctcg acgcagaccc gctcgtcgcc ctggacgacg tagaggctca
     4201 cgccctcgtc gtagatcgcg ttgagctcgc gcatgtaggg gagggccacc ttcctgatgt
     4261 cgacgttgga gaagcccagg gcgccgagct gggcgatctt gggccccagg gagtagcgct
     4321 ggttctccgc gctccgcgaa aggtagcccc gccgctccag cgaggcgacg atccgggagg
     4381 tcgtgctcat cgcgaggtcg atgcgcttgg agatctccgt gagcgaaagc tccaggcggc
     4441 ccggcgcgaa gcagtccagt atgtcgaggg cccggtcgac ggcccgcacg aagttgtctt
     4501 tctccggcat ggagccttcc tcgcaggatg gcagtgtgtt ccgcagggcg gaatcatagc
     4561 atggggtccg tccgttgaaa agcggttttc cccgggcggc cgcggaaggc cattcgtccc
     4621 caggtccgcc cttcgcccga atccgcccgg acgcgctatt aacacgcgca aaataccgtg
     4681 ctatactcgg gaaagccggg gacgcgaggc ccgggacgcc tgagcggcgc cggccgggct
     4741 ttccgtggtt gacagtttga tatagtagta tataattccg ccagatggaa gcggaaattc
     4801 aagatactgt ctgacggtat gggaacggaa gcgaaggccg tccggaagcc cggaagcggc
     4861 ccaagaacaa gtaggatcca gtcggccttg gccttggacc cctcgcgggg ctccggccgt
     4921 gccggcttat ctccatctcc acgagagaga aggacatgag cgaacaaaag aaggtgtttt
     4981 tccacgtcgt ccgagagaaa tggtgcaagg gctgcaacat ctgcgtcgcc ttctgcccga
     5041 agcaggtcct ggagctgaag ggcggcaagg tggccgtcgc gaggcccgag gactgcatcg
     5101 gctgcaggat gtgcgagctc cgctgtcccg actacgccat cgaggtgcac gagaagggtc
     5161 ccgagcccgc gccgggcaag gacgcggcca gccaagacta cgacctgccg ccggaggccg
     5221 accatgccta agaccatcaa gctcatgcag ggcaacgagg cctgcacggc gggcgccctg
     5281 gccgccgggc tccgcttcta cgcgggctac cccatcacgc cctccacgga gatcgccgag
     5341 ctctgctcgg aggagctccc gaagatcggc ggcaagttcg tccagatgga ggacgagatc
     5401 ggctccatcg cggcgatcat cggcgcctcc ctcgcgggca agaaggcgat gaccgcgacg
     5461 agcggccccg gcttctccct catgcaggag tgcctcggct acgcctccct ctgcgaggtg
     5521 cccatcgtca tcgtcaacgt ccagcgcatg ggcccctcca cgggcatgcc cacctccccc
     5581 gcccagggcg aggtgatgca gtcgcgctgg ggcacccacg gcgaccaccc cgtcatcgtc
     5641 ctcgcccccg gcaacgtgcg cgagtgcttc gacctcaccc tgcgggcctt cgacctctcg
     5701 gagaagtacc gcgtgcccgc catcgtcctc accgacgagg tcatcggcca catgcgggag
     5761 aaggtcgagc tccccgaccc cgccgacgtg aagcccctgc gccgcgccgc ccccgcgggc
     5821 aggaccggct acaagcccta cgcggccgac ccgaacggcg gcgtccccct catggcctcc
     5881 ttcggcgagg gctaccgcta ccacgtcacg gggctcttcc acgacgagac gggcgccccc
     5941 tcctccaaga ccgaggtggc cgacaggctc gtccgcaggc tccaggccaa ggtggacctc
     6001 cacgccgacg agatctgcga ctacgcctcc gagagcctgg aggactgcga ggtcgccgtc
     6061 atctccttcg gctcctccgc gatgtcctcc ctctccgcgg tccgcagggc ccgcaaggag
     6121 ggcgtcaagg ccggcatgct ccggctcaag acgatctggc ccttccccga gaaggccgtc
     6181 cgcgagctcg cgggccacgc gaagacgatc ctcgtccccg agatgaacct aggccagctc
     6241 gccctggagg tggagcgggt cgtcgccggc cgcgcgcgcg tcgtgcgcgt cggcaaggtc
     6301 aacggcgagc tcttccgccc ggaggaaatc cactccgccg tcatgggggc aaagtgatgt
     6361 ccgagatgct gagctacttc cgccaggagc ggcttcccca tatctggtgc ccgggctgcg
     6421 gccacggcac catcacgggc gccctggtcc gcgccatcga gcgcctgggc ctcgacaaga
     6481 acaaggtctg cgtcgtctcc ggcatcggct gctcctcgcg ggccgtcgga tacctggact
     6541 tcgacaccct ccacaccgcg cacggccgcg ccctggcctt cgccacgggc atcaagctcg
     6601 cgcggcccga cctgaaggtc atcgtcatga cgggcgacgg cgactgcacc gccatcggcg
     6661 gcaaccactt catccacgcc gcccgccgca acatcggcat cacgaccatc gtgatgaaca
     6721 acgagatcta cggcatgacg agcggccagt actcgccgat gacgcccaag ggcttcatcg
     6781 ggacgacggc gccctacggc aacctggagc cccacttcga cctctgcgag ctcgcgaagg
     6841 cctccggcgc cagctacgtc gcccgcgcca cctccttcca cgtgcccctc ctcatcgacc
     6901 tcatcgagaa ggccctccgg aacgacggct tctccgtcgt ggaggccatc gcccagtgcc
     6961 cgacctacct cgggcgccgc aacaagctcg gctcggcggt ggacatgctc aactggatca
     7021 aggaccagac catcaacgcc aaggccgccg ccgccctccc gccggagaag cgcgagggca
     7081 agctcctcat cggcgagctg cacaacgcgc ccgcgcccga gtacaccgcc tcctacggcg
     7141 agttgatcac gaaggtgcag cgcagcttcg agaacgtaaa gggagggctc tgggaatgcg
     7201 caccgaattc agactgagcg gctcgggcgg gcagggcctc ctcctggcgg gcatcgtcct
     7261 cgccgaggcc gccatcctcg aggacaagaa cgccgtccag acccagagct acggccccga
     7321 ggcccgcggc ggctccagca aggccgaggt cgtcgtctcc gacgaggaca tcgactaccc
     7381 gaaggccacc gaccccgact acctcctcgc cctcacggcc gactcctacc ggacctacgg
     7441 caagctgcag ggcaagggcc tcatcatcgt cgactcctcc gtcgccctcg atccggagat
     7501 caaggcgagg accgtgagcc ttcccatcct ggacacggcc gcgacgaagg tcggcaagaa
     7561 ggtcgtcgcg aacatcgtcg ccctgggcgt cctgggcggg atttccggcg tcgtgagccc
     7621 cgcgatcctg gagaaggcgg tcctgaaccg cgtccccaag ggcaccgagg agctcaacct
     7681 caaggcgctc cgcgcgggcc tcgagctcgc cgccggcgcg aaaaaggcct aagggccttt
     7741 tggagaaccg aagatgagcg acaacgagaa actgaagaag ggccgcgagg cctacgacgc
     7801 gggcgtcgag aagggccttt cgaagttccc cgagcgcagg aaggaattcg tcacgggctc
     7861 gggctccccg gtggggcgcc tctacacccc cctcgacctc gagggccgcg actacctggc
     7921 cgaccagggc ttccccggcg agtatccctt cacccgcggc gtgcagaaca cgctctaccg
     7981 cggccgcttc tggaccatgc gccagtacgc gggcttctcc aacgcggagg agtccaacaa
     8041 gcgctacaag ttcctcctgg agcagggcac gaccggcctc tccatcgcct tcgacctgcc
     8101 cacccagatc ggctacgact ccgaccaccc cctctcccag ggcgaggtcg gcaaggtcgg
     8161 cgtcgcgatc gactccctcg cggacatgga gatcctcttc gacggcatcc ccctcgacaa
     8221 ggtctccacc tcgatgacga tcaacgcccc cgcctccgtc ctcctcgcga tgtacatcgc
     8281 ggtggcggag aagcagggcg tgacgccgga caagctcaac ggcaccatcc agaacgacat
     8341 cctcaaggag tacgtggcgc gcggcaccta catctacccg ccctcgccct ccatgcgcct
     8401 catcacgaac atcttcgagt actgctcgaa ggaagtgccg aactggaaca cgatctccat
     8461 ctccggctac cacatccgcg aggcgggctc caccgcgagc caggaggtgg ccttcaccat
     8521 cgccgacggc atcgcctacg tcgaggcggc ggtcaaggcg ggcctggacg tcgacgactt
     8581 cgcgccccgc ctctccttct tcttcaacgc ccacaacgac ctcttcgagg aggtcgcgaa
     8641 gtaccgcgcg gcccgccgcg tctgggccaa gatcatgaag gagcgcttcg gcgcgaagag
     8701 ccccaagagc tggatgctcc gcttccacac gcagacgggc ggctccaccc tcacggccca
     8761 gcagccggac aacaacgtcg tccgcgtggc gatccaggcc ctggccgcgg tcctgggcgg
     8821 cacccagtcc ctccacacga acagccgcga cgaggccctg gccctcccca cggaggagtc
     8881 ggtgcgcatc gccctgcgga cccagcagat catcgcctac gagtcgggcg cggcggagac
     8941 ggtcgacccc ctggccggct cctactacgt ggagagcctc accaaccgga tcgaggacga
     9001 ggccctcgcg tacctgaagc gcatcgacga gctcggcggc gccgtgaagg cgatcgagca
     9061 gggctacgtc cagcaggaga tccaggactc cgcctaccgc taccagatgg acatcgagaa
     9121 gggcgaccgc gtggtcgtcg gcctcaacaa gttccaggtg aaggagaagg ctcccgaggg
     9181 gctcctccgg gtcgatccct ccgtggggac ccgccagtgc gagaagctcg cggccctgaa
     9241 ggccaagcgc gacaacgcga aggtgaaggc cgccctcgcc gtcctggaga cggccgcgaa
     9301 gggcgacgcg aacctgatgc cgcccatcct ggaggcggtc cgcgcctacg cgaccctggg
     9361 cgagatctgc gacgtgatgc gcggcgtctt cggcgagtac cgccccagcg tgatgttcta
     9421 agggaggccc gaaatggaac gcaagatacg tgtcctcgtc gcgaagccgg gccttgacgg
     9481 ccacgaccgc ggcgcgaagg tcatcgccag ggccctcagg gacgacggca tggaggtcat
     9541 ctacaccggc ctccgccaga cgcccgagca gatcgcccag gccgccgtcc aggaggacgc
     9601 cgacgtcgtc gccctctcca tcctctccgg ggcccacaac cacctcttcc cgagggtcat
     9661 cgagctcctg aaggaaaagg gcgcggacga cgtcctcgtg atcggcggcg gcgtcatccc
     9721 cgaggccgac tacgactccc tcaagtccaa ggggatcgcg gccatcttcg gcccggggac
     9781 gccgaccaag gcgaccatcg acttcatcag ggaaaacctg aagcgcaagt agcggggatt
     9841 ccatgaatac gcccaaggcg ggaggggcgg cctccgcgcg ccccctcgcc gagcgcgtcc
     9901 tcgcaggaga gcagcgggcc gtcgcccgcc tcatctcggg cctggaggac ggcgacccgg
     9961 ccgcggtcgg ggagatgcgc tccctgcgcg gccgcgcggg ccgcgcccac ctcatcggca
    10021 tcaccggccc gcccggctcc gggaagagca ccctcacgga caagctcatc ggggtcttgc
    10081 gcgcccgggg cgcccgcgtg ggcgtcctcg ccgtcgaccc ctccagcccc ttcacgggag
    10141 gggccatcct gggcgaccgc ctcaggatgc agggccacgc gacggacgag ggagtcttca
    10201 tccggagcct cgcctcgcgc ggacgcctcg ggggcctctc ccgcgcgacc caggccgcgg
    10261 cccgcgtcct ggacgcggcg ggctacgact acgtcgtcat cgagacggtc ggcgtcggcc
    10321 agtccgaggt cgacatcgtg aagatcgccg acacggtggt cctcgtctcc gtgccgggcc
    10381 tgggcgacga catccaggtc atcaaggccg gcatcatgga gatcggcgac gtcttcgtcg
    10441 tcaacaagtc cgaccgcgag ggctccgacc gcgtggtccg cgagatccgc ggcatgctgg
    10501 agacggcggc ggccctgaag cgggccgccc tgcccgaacc cgtcgacacc ctctcggggc
    10561 gcggcgcggc cggcctgggc gcggccggcc tgggcgcggc gacgggaccg ggcctgcccg
    10621 gcgcgggaca ggggcggcaa gcccccgcgc cgctttcggg agaggccctc tcccaccatt
    10681 tgactgaaag cgcgaatagt gtcaagctag cggacgattc cgggctaccg cccgtcctca
    10741 agaccgtcgc ggagacgggc gagggcgtca aggagctcgt ggacgccatc ctggcccaca
    10801 gggccggcct cgcggcctcg ggagccctgg aggcgaggcg gctcgagggc atccgcgcgg
    10861 agatgcgcgc cctcatcgcc ttccgcctcc tcgacgccct ggagggcggc gcgggccgcg
    10921 ccagggagga cgccctctcg agggcggtgc tgggcggcga cgccgacgtg tacggcgcgt
    10981 cggaggagct tttctcctcc atcgcgaaag gggaagcgaa atgaaggtac tgaaggtcga
    11041 tcacatcggc gtggccgtcg agaagctcga cgaggccctc gccttctata cgggcgtcct
    11101 gggcctcgag ctccacggga ccgagaccgt ggaggagcag aaggtccgca cggccttcct
    11161 cccgatcggc gggacggagg tcgagctcct ggagtccacc gaccccgagg gaccgatcgg
    11221 caagttcatc gccgcgaagg gcgcgggcat gcagcacatc gcctaccgcg tggacgacat
    11281 cgaggcggcc ctggccgagc tcaaggccaa gggcgtccgc ctcatcgacg agaagccgcg
    11341 ctacggcgcc ggcggggcga ggatagcctt cctccatccc aaggcgacgg gcggcgtcct
    11401 cgtcgagctc tgcgagcgga aataactcaa gggatcccca tcgatccaac aaggacggtt
    11461 tcatatatgg acgacaagat caaggaactt gcggagcgcc gcaggaagat cgaagagggc
    11521 ggcgggccca agcgcgtcgc cgcccagcac gagaagggca agatgaccgc gcgcgagcgc
    11581 atcctctccc tcctcgactc cggcagcttc acggagatcg acgccttcgt cgagcaccgc
    11641 tgcacggacc tcggcatggc cgaggtcgag gcccctggcg agggcgtggt caccggctac
    11701 ggcacggtgg acggccgcct cgtctacgtc ttcgcgcagg acttcaccgt gatcggcggc
    11761 tccctgggcg ccatgcacgc ggcgaagatc tgcaaggcca tggacatggc cgtgaaggtc
    11821 ggggcccccg tcatcggcat caacgactcg ggcggagccc gcatccagga gggcgtcgac
    11881 gccctctgcg gctacggcga catcttcttc cggaacgtga aggcctcggg cgtggtgccc
    11941 cagatctccg tgatcatggg cccctgcgcg ggcggcgcgg tctactcgcc ggccctcacc
    12001 gacttcgtct tcatgacgga gaagacctcc aacatgttca tcaccggccc ccaggtcatc
    12061 aaggccgtca cgggcgagga cgtcaccgcg gaggccctgg gcggcgccca cgtgcacgcg
    12121 gcgacgagcg gcgtggccca cttcgcctat cccgacgagg cccagaccat cgccgggatc
    12181 aagaagctcc tctccttcct gccctcgaac aacatcgagg acgcgcccct cgcgccctgc
    12241 gccgacgacg tgtcgcgcat cgacgaggcc ctggaggaga tcatccccga gtccccgaac
    12301 aagccctacg acgtccgcga cgtgatcgcc cgcatcttcg actccggcga ctatctcgag
    12361 gtccagccca tctacgcgca gaacatcgtc acctgcttcg ctcgcctcgg gggccgctcc
    12421 gtgggcatca tcgcgaacca gccgaagtcc atggccggcg tcctcgacat caacgcgagc
    12481 gacaaggccg cgcacttcat ccgcttctgc gactccttca acatccccct cgtgaccctc
    12541 gtggacacgg gcggctacct ccccggcgtc ggccaggagc acggcggcgt catccgccac
    12601 ggcgcgaaga tcctctacgc ctactccgag gcgactgtgc ccaagatcac cgtgatcctg
    12661 cgcaaggcct acggcggcgc ctacatcgcg atgtgctccc gccacctggg cgccgaccag
    12721 gtcttcgcct ggccgaccgc ggagatcgcg gtcatgggtc ccgacggcgc ggcgaacatc
    12781 atcttcaaga aggagatcga cgagtcctcc gaccccgcgg cgacgcggaa ggagaagatc
    12841 gccgagtaca agaagaactt cgccaacccc tacaaggccg ccgtccgcgg ctacgtggac
    12901 gacgtcatcg acccggagga gacgaggccc cgcctcgccc gcgccctgtc gatgctcctc
    12961 gggaagcgag agtcgggccc cgcgcgcaag cacgggaaca tccccgtcta agggggcgcg
    13021 ccatggacca gtggtcgctc accgcgctcg ggatggggac ggtcttcctc accctgatct
    13081 tcctcgccct gatcgtgtcc ctcttccccc tcttcttcag gaagcccgag aagaagcgca
    13141 aggatcccgc gccccttccc tcgctctcct cccaggccgc gccggccgcg gccccctccg
    13201 gggccgccgg ccccgagctc gtcgccgtca tcgccgcggc cgtcgccgcc gcgagcggct
    13261 cggcccccgg ctcgttcagg atcgccagca tcgcccgctc cgagagctcg ggcggattca
    13321 acacccccgc gtggggccac gccgaccgcc taggccggac cagcttcaat tcctagagga
    13381 gtaaccgcca tgaagaaata ccgcgtaacc gtcaacggcc agagctacga cgtcggcgtc
    13441 gaggagctcg gcgccgctcc ctccttcgcc gcccccgcgg ccgccgcggc tcccgttccc
    13501 gccgccgcgc ccgccgcgcc cgcggccccc gccccgagcg ccggccccgc cgtcgctggc
    13561 gccaccaccg tgaaggcccc gatgcccggc acggtcatga gcttcaaggc catcgtcggc
    13621 cagcacgtga agcgcggcga cgtcctcctc atcctcgagg ccatgaagat ggagaacgag
    13681 atcgtggccc ccgccgacgg caccgtcgcc tccatccgcg tcccccaggg cgcggcggtc
    13741 aacacgggcg acgccctcgt cgatctttcc taaggcgccc ctatgtggga aggcatactt
    13801 agcttccttc aatccacggg ggtggtgagc atcacgagcg gccagatcct catgctggcc
    13861 gtctcctgcg tcctgctcta cctcgggatc tacaggaagt acgaaccgct gctgctcgtg
    13921 cccatagcct tcggcatgct cctcgcgaac ctgccgatat cgggcatcac cgacgggccg
    13981 gcgaacggcc agccgggcgg cctcatctac tacctctacc agggagtcaa gttcggcatc
    14041 tacccgcccc tcatcttcct gggcgtgggc gcgatgacgg acttcgcccc cctcatcgcc
    14101 aacccctcga gcctcctcct gggcgcggcg gcccagttcg gcatcttcat ccccttcgtg
    14161 atcgccaact acatcggctt cgacatgaag tcggccgctt gcatcggcat cataggcggg
    14221 gcagacggcc cgaccgccat cttcctcacc tcgcggctca atcccgacat gctcggaccc
    14281 atcgccatcg cggcctactc ctacatggcc ctggtgccga tcatccagcc gccgatcatg
    14341 cgcgccctca ccaccaagaa ggagcgggcc gtcgtgatgg gccagctccg cgacgtctcc
    14401 aagaccgaga aggtcgtctt cccgatcgcg gtgacgatca tcatcggcct catcctcccg
    14461 ccggccgtgc ccctcgtcgg ctccctcatg ctcggcaacc tcttccgggt gacgggcgtg
    14521 accgagcggc tctccgacac ggcccagcac gccctcatca acatcctcac gatcttcctg
    14581 ggcgtcgcgg tgggctcgac ggcgagcgcg gagcgcttcc tcaaccccca gaccctcaag
    14641 atcatcgccc tgggactctt cgccttcgcc tccggctcgg cgatgggcgt catcttcggg
    14701 aagatcatgt acctggtcac gggcggcaag gtcaacccgc tcatcggctc cgcgggcgtc
    14761 tccgcggtcc cgatggccgc gcgcgtctcg caggtggtgg gccaggagga gaatcccggc
    14821 aactacctcc tcatgcacgc gatgggcccg aacgtcgcgg gcgtcatcgg ctccgccgtc
    14881 gccgcgggcg tcctcctctc cgtactcggc taggccggtc cccgcacaat cgacaccgta
    14941 cccgaaccgg gtctcccctc gcaaagaggg gagactcgcg gcgggtttcg taaagcagca
    15001 tagggcgggt cctgcccctg cctatgcgac cccgggcgct tcgcgccggg gcgcgcatag
    15061 gcaggggcac ccgccgtccc gctccgcctg cgataccgcg gccgggttcc atcgccttca
    15121 cgccgcgagc atgccggggg acgccctcgc cgcagggcgc gaagccggaa ggcacgcgtc
    15181 cgggctggcg cccggacggc gcggggtgat ggtcggcgcg cgggcacgcg tccgggcttc
    15241 gcagaaaccc tcggagtgtg cgcgggacct tactgagtgt gcgcggtaca ctcccgagtg
    15301 tggcgggtac cttacggagt atgcgcggca cactctcgag tatgttgagt acactcccga
    15361 gtgtgcgcgg aacacttcgg agtatgcgcg gcacattccg gagagtgtac ggaacactct
    15421 ggagtgtgct tggtacactc cggagagtgc gagtcacatt cgagagcgtg cgcggaacac
    15481 ttcggagtgt gcgcggtact ctcaggagtg tgcgcgctac actcccgagt gtcggcggaa
    15541 aactccggag tgtgtataga ccgacaacat aggtgacaaa caatccggga acataggtta
    15601 cattccgaag catcggggtg ctggggcgaa gcattggttt gagcatccgg gacaatggta
    15661 accaggaccg aaagatcggc cgcatggacc ggagcatcgg gggcttggac cacctcacgg
    15721 ccggccaaga ccgatcgata ggtccgattg cccatcgaca taggcgccgc gccgaaggcg
    15781 cgcgttggcg cgggcaaagg caacgccatc c
//
LOCUS       DAOOSJ010000025        15750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2536_length_15750_cov_5.860147, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000025 DAOOSJ010000000
VERSION     DAOOSJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_15750_cov_5.860147"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..5230
                     /locus_tag="PLG14_02500"
     CDS             161..5230
                     /locus_tag="PLG14_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48031.1"
                     /translation="MTDRNGASASIGPTYFIVDSQNPALSLTTPAAGAKVKEGNLVIA
                     GTATDGGGFGANPLSITLRHSDTGSPLHNQVLNPAVSGGVFSQSVPIDAASKDGTLYM
                     DLVLADRAGKQASITRAVTIDTTAPSLSLNYPSHAAYINGLVSVTGTADDLSGLADVT
                     LQILDPATKLPVAGLTRGTTLSAWDFSLNAETYASATYGVDVNADGKLWKVFFRLAAT
                     DNSGNVTEYKVGDPLDWPYFFIDTDGDKPTISVTQPKNGDKIGGFVNMFGTATDDDGA
                     VMQVEVQIDFNGDGDFLDSRDLDNSDGDSNPLTGIQSGVDTDILGTTVRIGDGAHKWE
                     DESAWYVVPVSNNAWTEELNSANELYASNTGGTGNITIRARSRDMFGLASEISSRTIT
                     LDQNFPRIENVTPLDQSYQSGVFDLFAEFGDNVDLTLGPVAQIRINVNKTGYQTLVEG
                     LNTGAGHPYALVADLAEPQNGYDLTFPIDTNSYFNNSSGILYVDLYVRDLANNVNQKT
                     LTFYVDNQDPSSSWSDSSTRPDGSSLRNGMITVNGAAKTYVEANYGDSGSVSGISRIE
                     AYVVRGGQVRNLKANGVWAGTPTTESAAIETYSLADNTWTAGTNPAAPIVTHAALGSD
                     YVIKVDKATEMSDLILGTDTDADGYHEYLGIYEGKARWRAYFDSQYLPDGLADLHYVV
                     YDLAGNYVHRMRQVFFANNGPRFDQVRAGSDLNSDGSVANPDLVAKTGLSLSGADRPS
                     AASFADASLAGNALVAVGQRITVNGASRGAISAWDSVSGLIAWDGAALATGTPVTSYS
                     ITEIEEMKDYYNIETALDRSDYLRAKNGKLRLAVFASDPTGSNQGVKKVFVDVYDASG
                     TVKFGSPQATGYDPVDGDIDVTLSPTPGSAPWNTAYSGGLANFNLKVTIRDNDDINVS
                     RWVKARIVDPADSTKPVVTLSPLAQADQLSSVGHLELKGDSSTSAWTAIAAAYGDDDP
                     KASGTIVVRGTVFDENRVTEVSGAAEGIPQTLLASWSGGALAAATGAFEITGQTLGET
                     GHTVSFAYSWNTADVTGVAGLDKSLAFGARDGAAAPNAADPKSQRVDVVPYIKAIARD
                     SLNYNTSRSRLGSFSLRQGEPVTVTGFNLFNTTADTVSLPGAATTLSAGSTPESFTVT
                     LAAAATSGPVAVTVSGIVSVNNLNDNTKAWNTEVDPADTASALWTDDRKVHVWRSDDL
                     QAGSNRGYFTGSVDPEYPAMTADNTGVLYGSWSNYATSNVYWGTNAAAANEIYHSYDP
                     LEHTDIDYGARQTVVYNANTYGNNTWNQDGAGGVQIWDSQSTANPPDGTGVGDFYNME
                     ELYHDEKLMQFINPRVVGSGGAIHISYFDTDTKALKYAYQRSGSVFTGENRGWISIDG
                     GYDEDDTGTGALASVMGTGGTTRTVLSVAVAIGDYVSIGDRIVQRSDGTWILSTAQGQ
                     IETVIAVGANVNSSIVVATVRDLDRVVDKGGSSRAASAGEYSAIDVMPTSGFPVIVYY
                     DITNQTVRLARASAVNPTASQWVLQTVMSSGTDPNFKYSGKYISMRIDSAGYVHLAFY
                     RNSTGDLVYMKSTNNPTDGSAYVFGKSVIVDSVGSVGIWADVSLDGTNPVLSYLDSSM
                     VNTFDGVKVAYYDPTLETLSGDTAGQPDTIDGWETMNAALGYEVESVRTSVEVDTGAN
                     FWHAAVGYGSSDYFRIGYYVK"
     gene            5280..6221
                     /locus_tag="PLG14_02505"
     CDS             5280..6221
                     /locus_tag="PLG14_02505"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOX48032.1"
                     /translation="MARIALLGALAAPLFLGACGGAGGLRSASKPFEAALEDPATPPA
                     SKRLLSTVREIAAFGAEAYGLGREAAALRYLPGPRSAPAWRLTLAAPRSVAPRSVAYF
                     VDEAKARKAAEKAEGEGAGAFLEPCDSLWPLGLAPGPLVESDAALGYDRLAERVFREL
                     FYAAYGKRGDEASRESFATFAAEEATAAYLASKLTPASPLLGNYLAEKRDERTFAALF
                     PDYRSRFEGLYAQSPLPSDWVTLRDFLSEAWLQDYRANYPNRFITNKFKDFGRKMPSD
                     AELAAWSDPFAGRAAWEKRLTELAGDFPALVRRELGR"
     gene            6324..13568
                     /locus_tag="PLG14_02510"
     CDS             6324..13568
                     /locus_tag="PLG14_02510"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOX48033.1"
                     /translation="MPLAPRSFGPRGARRALLALALCLAALPAAAMGGKEPPREKASG
                     LEDWEHGFDLSGLPAGKYNIVVEGKDKAGNVSIAGPINVYVDPASDLPRVSIVNPLPL
                     MRVGGDLNVVGTCVDDDGVARVEVSVDGGEFVPAEGGEFWSLYLGTKDLADGKRIIAA
                     RGVDINGLSGPEVRVSFNLDRAKPLASVAAPEAGALVAGAIRLEGTVSDANGVASLEI
                     SMDGGSSFSKVELKKSKEAAAASFAYSLDTRKLPDGPRVVWMRSVDGVGSKGSAAFLL
                     VVDNTKPAIEILRPAKEKAVNGRFALAGAVRDAVGVRRLSYEFGGAEKGEIELTPGNP
                     YFVKELDSGALKGDKAEIVLLAEDRIGNVTRLALAPKVDRKADKPVVRVSYPASGGQA
                     RPGESVWGAIADDDGAAMVRFSFDGGAPSELPASEHFAIPLPELASGRHVVSITAVDV
                     EGAAGDPLVLPFVMDRGPGAVSFLRVSGGAAKQAAGKAAPAEGARDYASGVEVALDSG
                     SLLEGRVESANPPLSAEWSVAGGAPRKLELSKAAEPGAYSFRLPLDRSLPYGFAPIEV
                     KVADGFGNSYSGRALVYATNLGAIREDSGFRFDDPRIGEGGRVVLSAAEPLLGAFYRE
                     ELASLRLEPATDIVAASFDGRVVSLAVLKEGATPPLRLVGKTKRGHDFEAGPFSFACD
                     SIPPRIEIESPAEGAWHSGRFEAKGRVVDSGGLAKLLVRSSPGAEARELKVGSDGRFS
                     FFVEPAASAAAAGGIAPAEAGSAGPSYLEVEAVDSSGNAARAYRSYGVDAKAPALRFL
                     SPEAGAQVSGPEDIAAVAEDASGLASVEYAADGASFTPVERRGNAFIHRADLAANPKA
                     AYRVTDRAGNAAVLRPEVKAVPAAKPLPAADSVSVEPEAGEARLELAGAAGTGAAARK
                     LSLLLPALSEEAFSALGLAEGKEAPERFSVRLLASGALSLKGKVASEGKVKALSLSLD
                     GGASWAPLFQAKDAKSILPEAAVALNVDTAKLPDGEARLVLKVEGEAGGLSYVPLYLL
                     VDNRAPEAAILAPEASVAALPGPYPLVLKISDPPGPSSGAGVTALTSGGGAFSGVAAA
                     ELSLGADKKALPVAEGGSYYAFWADPEAAAAAAKGAALAVWLSARDAAGNAAAATRKL
                     AYDAAADAPRITLSLPAPAAVAAPAPGAKPAPAATERLVLSPGDAIAGLAADDDGPAE
                     LRLSFDGGEALVFPAGAFALALPALSPGRHSLKLEARDQGGKSAEAKRELLVRGPAPS
                     FSGVEAGAASGRKAWLPGLELALASGSALSGRVEAPNGLSSLEYSVNGGAAQKALLGK
                     AEAGGAAAAALPFSAALPASLPYERFVVELVARDLSGLETRRRYDFHAVLPSLPGAPP
                     ADDAEGLRFHDARVSAEGEALRLLLKPGERLSGRFNGRPLASLAVEPSVGFLSASFEG
                     ASVSLEAKAEGLSGPPYAAAQGALRLVAVTVDGDRFEWGPFSLAVDAASPSLALDSPA
                     DASWTKGPTRIAGRAEDPNGVVAVELSVNGGPRTRLEPRAAAPAATPPAAPAAPAASA
                     AKAPAAKPAAAPAPAPSASAAPAPKGPLAAYTFDAELPLAEAEDGIIRLELFARDSAG
                     RESSLTRFVYKDSQPPTLSLVLPPPGEAVNGTTTLVGEAADSGRLASASFSAAKGAAA
                     EEVLGLSVFSRTVDLARVAFPLPEGYGFAVADRAGNVAVLSPELLVDKEKDKPVVEIA
                     APAELEVIRADFFVSGTAFDDDGVAAIEYRLDGGEWVRVEPKGAGFSVPRTLAETADN
                     EHLVEAFAKDLYGVKGDTVARRFRVSKEEPLARMTSPSIAKPQRGLVELAGEASDANG
                     VASVRLSFDNATAFNLGSGAESWRYPLDTRVLADGLHAIAAKPLDKYDTEGFYATLLN
                     VDNTPPRAELSLPADGASYSGTLPLSGRVSDNVRLDSARVEVAPIGQGRPPVLVVDLG
                     QAAVVRRELDVSALPPGGYTVRLVAKDRADNETLASRDVVLKEGRPADMVEILFPVEG
                     EELSGAARLYGRAAVSGGVSAATILVDGADVGTAAVDARGWFAFDLPADKLEEGTRRI
                     VARAAASDGRLVESRAETVSWKRLGPWVSVDSLPAGAYLPYRPYLSGGAGYLAAAPAA
                     GDKAAQAAFKKTEGQRRVTRVELSLDNGRTFLPAKGGERWKFRLETQDYPEGSLHVIV
                     RASFGDGSTAVAKTILALDKTPPEVALERPQENGRYNAEIRAAGVASDESGLESVGLA
                     LRKGDKRGYELPAFIQGLYIDGHFLGATYYDIGAGLSFFQDNVKLQVMTGMAPEEGRF
                     GGSVMGAKLLANIAYLPANFLLGPDWNFLSASLALGANFSYFSQSASGSGLILGAVVA
                     QLEFPKITVQDWSFLKKYSFYTEGQAWFVSSDVQGGILFRLSFGARLGLF"
     gene            complement(13786..14700)
                     /locus_tag="PLG14_02515"
     CDS             complement(13786..14700)
                     /locus_tag="PLG14_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOX48034.1"
                     /translation="MRLGLFTAAFPGLALEPLADWAAASGFEALELAAWPVSRAERRY
                     AGVSHVDAAGLDKAGAAAVRKALASRGLGISSLGYYPNPLDPDPAVSGPAIEHLKKVI
                     DAAARLEVPVVGTFTGRDWRKGLDENLEAFARTWPPIVRYAAERGVKIGIENCPMLFS
                     ADEWPAGKNLAVSPAVWTRMWEIIPDENFGLNLDPSHLVWQMIDCERVVRDFGSRIFH
                     VHAKDLMIDREGLYRNGVLSQGMGWAVPRLPGLGDMDWRRFVAALYRSGYDYVVSVEH
                     EDRNFEGSEELVKRGFLIARDALRPWIH"
     gene            complement(14697..>15750)
                     /locus_tag="PLG14_02520"
     CDS             complement(14697..>15750)
                     /locus_tag="PLG14_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX48035.1"
                     /translation="ARKARELGIERAYPSYEAALADPAVSVVHLAVPNALHAPYAEAA
                     LAAGKHVICEKPLAMSSAESARLAELARRSPGVAAVNYNLRFYPLVREARERVARGDL
                     GSPFLVHGAYLQDWLLLDTDWNWRLEPEAGGALRAVADIGTHWLDMAAYATGRRIEAV
                     LADFATFHPVRRRPRGPVETFSGKTGAPSGELVPVAVASEDAATILLRFEGGLRGSLV
                     VSQVSAGRKNSLHFEIDGSASSLAWDSEEPNALWLGARDEPNRLLMKDPALLTPAARR
                     LVSYPGGHQEGYADTLKQMFASFYAYVEAGDYAAPRDFPTFDDGHEEMLLCDALLESS
                     RKGAWVELPRRKGGQA"
BASE COUNT         2463 a         5645 c         5269 g         2373 t
ORIGIN      
        1 gggcacgatc tccgacgacg acggcttcat ggcgagcgcg gtaagcgtcc tcctggacgg
       61 cgccacgaca ctcaccccga caagcccgag cggcacgacc agtccatggg agtatacctg
      121 ggcgagtctc gctgaggggg agcactacct tgcgataacg gtgaccgacc gcaacggcgc
      181 ttcggcctcc atcggtccga cttatttcat cgtcgacagc cagaatccag ccctctcgct
      241 cacgacaccc gccgccggcg caaaggtgaa agaaggcaat ctcgtcatcg ccggcaccgc
      301 gacggacggc ggcggattcg gtgcgaatcc cctttctatc acccttcgcc acagcgacac
      361 gggatcgcca cttcacaacc aggtcctcaa tccggccgtg tccggcggcg tcttcagcca
      421 gtctgtcccc atcgacgctg catcgaagga cggaactctc tacatggatc tcgtccttgc
      481 cgacagggcc ggcaagcagg cctcgatcac ccgcgcggta accatcgaca ccaccgcgcc
      541 ttccctaagc ctcaactacc ccagccatgc ggcctatata aacggcttag tctcggtgac
      601 gggcacggct gacgacctta gcggcctcgc ggacgtaacc ctccagattc tagacccggc
      661 caccaagctc cccgtcgcgg gcttgacgcg tggcaccacg ctctccgcct gggacttctc
      721 cctcaacgcg gaaacctacg cttccgcgac ctatggcgtg gacgtgaacg ccgacggcaa
      781 gctctggaag gtcttcttcc gcctcgccgc gaccgacaat tccggaaacg tgaccgagta
      841 caaggtgggc gatcccctcg attggccgta cttcttcatc gacaccgacg gcgacaaacc
      901 cacgatatcc gtgacccagc ctaaaaacgg cgacaagatc ggcggcttcg tgaacatgtt
      961 cggcacggcc accgacgacg acggcgcggt tatgcaggtc gaggtccaga tcgacttcaa
     1021 tggcgacggc gacttcctcg acagccgcga cctcgacaac agcgacgggg attcgaatcc
     1081 cctcactggc atccagtccg gcgtggacac cgatatactc ggcacgacgg tcaggatcgg
     1141 ggacggggcc cacaagtggg aggatgagtc cgcctggtac gtcgtgcccg tgagcaacaa
     1201 cgcctggacg gaggagctaa actccgccaa cgaactctac gcttcgaaca ccggcggcac
     1261 ggggaacatc acgatccgcg cgcggagccg cgacatgttc ggcctcgcga gcgagatctc
     1321 ctcgcgcacg atcaccctgg accaaaattt cccccgcatt gagaacgtca cgccgctcga
     1381 ccagagctac cagagcggcg tcttcgacct cttcgcggag ttcggcgaca acgtcgacct
     1441 caccctcggc ccggttgccc agatacggat caatgtcaac aagaccggct accagaccct
     1501 ggtcgaaggc ttgaacactg gagccggaca cccgtacgcc ctggtcgccg acctcgcgga
     1561 gcctcagaac gggtatgacc tgacgttccc gatcgacacg aacagctact tcaacaacag
     1621 ctccggcatc ctctatgtgg acctctacgt gcgtgacctc gcgaacaacg tgaatcagaa
     1681 gaccctgacc ttctatgtcg acaaccagga cccaagctcg tcctggtccg actcaagcac
     1741 gaggcccgac ggatcgagcc tccgcaacgg catgatcacg gtgaacggcg cggctaagac
     1801 ctatgtcgag gccaactacg gcgactccgg atccgtgagc ggaatctcgc gcatcgaagc
     1861 ctacgtggtc cgaggcggcc aagtccgcaa cctcaaggcg aacggcgtct gggccggaac
     1921 gccgaccacc gaatccgcag caatcgagac ttatagcctc gcggacaaca cctggacggc
     1981 gggaacgaat ccagcggcgc ccatcgtcac gcacgccgcc ctcggctcgg attacgtcat
     2041 caaggtggac aaggccaccg agatgagcga tctcatcctc ggcacggaca cggacgcgga
     2101 cggctatcac gagtatctag gcatctacga ggggaaggct cgctggcgag cctacttcga
     2161 cagccagtac ctgcctgacg gcctcgccga cctccattac gtggtgtacg acctcgccgg
     2221 caactatgtg caccgcatgc ggcaggtctt cttcgcgaac aacggtccgc ggttcgacca
     2281 ggtgcgggcc ggctccgacc tcaactcgga cggatcggtc gccaatcccg acctggtcgc
     2341 gaagaccggc ctctccctct ccggggcgga ccggccgagc gcggcgtcct tcgcggacgc
     2401 gagtctcgcc ggcaacgccc tggttgccgt cgggcagcgc atcaccgtga acggcgcgag
     2461 ccgcggagcc atcagcgcct gggactccgt ttccggcctc atcgcctggg acggcgccgc
     2521 tctcgcgacg ggaacccccg tgaccagcta ttcgatcacc gagatcgagg agatgaagga
     2581 ctactacaac atcgagactg ctctcgacag atctgattat ctcagggcta agaacggaaa
     2641 gctccggctc gcggtcttcg cgagcgaccc cacgggcagc aaccaggggg tgaagaaggt
     2701 cttcgtcgac gtatacgacg cctccggaac cgtcaagttc ggcagtcccc aggcaaccgg
     2761 ctacgatcct gtagacgggg acatagacgt gacgctctcc ccgacgccgg gcagcgcccc
     2821 gtggaacacc gcctattccg gcggcctcgc caactttaac ctcaaagtca ccatccggga
     2881 caacgacgac atcaacgtct cccgctgggt gaaggcgcga atcgtcgatc cagccgactc
     2941 cacgaagccg gtcgtgacgc tttcgccgct agcgcaggcc gatcagctgt catccgtcgg
     3001 ccacctcgaa ctcaagggcg atagctcgac atctgcctgg accgccatcg cggcagccta
     3061 cggggacgac gacccgaagg cctctggcac gatcgtcgtc aggggtaccg tcttcgacga
     3121 gaaccgcgtc actgaggtca gcggcgccgc ggaaggcatt ccccagaccc tcctcgcaag
     3181 ttggtcaggc ggcgccctcg cggccgcgac cggggccttc gagataacgg gccagacctt
     3241 gggcgagacc gggcatacgg tgagcttcgc ctattcctgg aacaccgcgg acgtcactgg
     3301 cgtcgcgggc ttagacaaga gcctcgcttt cggcgccagg gacggcgcgg cggcgccgaa
     3361 cgccgcggat ccgaagagcc agcgggtgga cgttgtgcct tatataaagg cgatcgcgcg
     3421 cgattccctc aactacaaca cgtcgcgctc gcggctaggc tccttctcgc tccgccaggg
     3481 cgagccggtg actgtcacgg gcttcaacct cttcaacacc acggcggata cggtctcctt
     3541 gcccggcgcc gcgacgaccc tctcggcggg ctcgactccg gaaagtttca cggtgactct
     3601 cgcggccgcg gcgacgagcg gccccgtcgc cgtcacggtg agcggcattg tctcggtcaa
     3661 caacctgaac gataacacca aggcctggaa cacggaggta gacccagcgg acacggcttc
     3721 cgcgctttgg acggacgacc ggaaggtcca tgtatggcgc tcggacgacc tccaagcggg
     3781 ctccaaccgt ggctacttca ccggcagcgt agatccggag tatcccgcaa tgacggcgga
     3841 caacaccggc gtcctgtacg gttcatggag caattatgcg acttcgaacg tgtattgggg
     3901 cacgaacgcc gcggcggcga acgagatcta ccattcctac gatccgctcg aacacaccga
     3961 tatcgattac ggcgcacgcc aaaccgtcgt ctacaacgca aacacctacg gcaacaatac
     4021 ctggaaccag gatggcgccg gcggcgtgca gatatgggac agtcagtcaa cggcaaatcc
     4081 gccggacgga actggtgtag gggacttcta taacatggag gaactgtatc acgacgaaaa
     4141 gctcatgcag ttcatcaatc cgcgggttgt cgggagcggc ggcgcgatcc acatttcata
     4201 cttcgacacc gataccaagg cgctgaaata cgcatatcag cgtagcggat ccgtattcac
     4261 gggggaaaac cgcggctgga ttagcatcga cggcggctat gacgaggatg atactggaac
     4321 gggagccctc gcctctgtca tgggtaccgg cggcacaact cgtacggtct tgtcagtcgc
     4381 cgtcgcgatc ggcgattatg tatccatcgg agacaggatc gtccaaagaa gcgatggcac
     4441 atggatatta tccacggccc aaggacagat agaaacggtg attgctgttg gcgcgaacgt
     4501 gaacagctca attgtcgtcg caactgtaag ggatttggac cgtgtcgtcg acaagggcgg
     4561 ctcgagccgc gccgcctccg ccggtgagta ctcggcgata gacgtgatgc cgacgagcgg
     4621 attcccggtc atcgtctatt acgacatcac gaaccaaaca gtgcgcttgg cgcgcgcgtc
     4681 ggcggtcaat cccacggcct cgcagtgggt cctgcagact gtgatgtcct cgggcaccga
     4741 ccctaatttc aagtactcgg gcaagtacat ttcgatgcgg atcgactcgg cgggctatgt
     4801 ccatcttgcc ttctaccgca acagcacggg cgatctcgtc tacatgaagt cgaccaataa
     4861 cccgacggac gggtcggcct acgtcttcgg aaagagcgtg atcgtcgact cggtcggctc
     4921 ggtcggcatc tgggcggacg tctcgctcga cgggacgaat ccggtgctct cctacctgga
     4981 ctcctcgatg gtcaacacct tcgacggagt gaaggtcgcc tattacgatc cgacgctgga
     5041 gaccctctcc ggcgacacgg ccggccagcc ggacacgatc gacggctggg agacgatgaa
     5101 cgccgcgctc ggctacgagg tggagagcgt gcgcacgagc gtcgaggtgg acacgggcgc
     5161 caatttctgg cacgccgcgg tcggctacgg ttcgagcgat tacttccgca tcggctacta
     5221 tgtgaagtga agaggaagga gtacggatct tgacgcatca tagtaagaac ggcggacgga
     5281 tggccagaat cgcactgctg ggggcgctgg ccgcgccgct ttttctcgga gcttgcggcg
     5341 gcgcgggagg gcttcgctcg gcttcgaagc ccttcgaggc ggcgttggag gatcccgcga
     5401 ctcccccggc ttcgaagcgc cttctttcca ccgtccgcga aatcgccgcc ttcggcgcgg
     5461 aggcctacgg cctgggtcgg gaggcagcgg cccttcgcta ccttcccggg ccgaggtcgg
     5521 ctcctgcttg gcgactcacc ctcgcggctc cccgctccgt cgccccgcgc tcggtcgcct
     5581 acttcgtcga cgaggcgaag gctcggaagg ccgcggaaaa ggcggagggg gagggcgctg
     5641 gggccttcct cgaaccctgc gacagccttt ggccccttgg gctcgcgccc ggtccccttg
     5701 tggaatcgga cgccgcgctc ggctacgaca ggctcgcgga gcgcgtattc cgcgagctat
     5761 tctacgcggc ctacggcaag cgaggggacg aggcgagccg cgaatccttc gccaccttcg
     5821 ccgccgagga ggcgaccgcc gcctatctcg cgtccaagtt gacgcccgct tcgcctctcc
     5881 tcggaaacta cctcgctgag aagcgcgatg agcggacctt cgcggcgctc ttcccggact
     5941 accgctcgcg cttcgagggg ctctatgcgc agtcgcctct gccctcggat tgggtgacgc
     6001 tccgggactt cctttcggaa gcctggctcc aggactatcg cgcgaattac ccgaacaggt
     6061 tcattacgaa caagttcaag gatttcgggc ggaagatgcc aagcgacgcg gagctggccg
     6121 cgtggagcga ccccttcgcc ggacgggccg cctgggagaa gcgtttgacg gagctggccg
     6181 gcgatttccc cgccctcgtc cgccgcgagc tcggccgcta aacggcgggg agagccgcgg
     6241 ggaaccgcgg ctctcccggt tttgcgacgc ttcaagcgtg tcgatactgt aatgtaggaa
     6301 gttccgctcg ataaggaagg cctatgcccc tcgccccgcg ctccttcggc cctcgcggcg
     6361 cccgccgcgc cctcctcgcc ctggccctct gcctggccgc cctccccgcc gccgcgatgg
     6421 gaggcaagga gccgccccgg gagaaggcct cgggcctcga ggactgggag cacggcttcg
     6481 acctctcggg cctgcccgcc gggaagtaca acatcgtcgt cgaggggaag gacaaggcgg
     6541 ggaacgtctc catcgcgggg ccgatcaacg tgtacgtcga cccggcctcc gacctgcccc
     6601 gggtctccat cgtcaacccg ttgccgctca tgcgcgtcgg cggcgacctc aacgtggtcg
     6661 gcacctgcgt cgacgacgac ggcgtcgcga gggtcgaggt gtcggtggac ggcggcgagt
     6721 tcgtccctgc ggagggcggg gagttctggt ccctctacct cggcacgaag gacctggccg
     6781 acgggaagag gatcatcgcg gcgcggggcg tcgacatcaa cgggctatcg gggcccgagg
     6841 tccgcgtctc cttcaacctg gaccgggcca agcccctcgc ctcggtcgcc gcgcccgagg
     6901 cgggcgccct cgtcgcgggc gcgatcaggc tcgaggggac ggtgtcggac gcgaacgggg
     6961 tcgcctcgct ggagatctcc atggacggcg gctcgtcctt ctccaaggtc gagctcaaga
     7021 aaagcaagga ggcggctgcc gcctccttcg cctattccct ggacacgcgc aagctcccgg
     7081 acggcccgcg cgtggtgtgg atgcggagcg tcgacggcgt gggctcgaag ggctcggccg
     7141 ccttcctcct cgtggtcgac aacaccaagc ccgcgatcga gatcctgcgc ccggccaagg
     7201 aaaaggcggt gaacggccgc ttcgccctcg cgggagcggt gcgcgacgcc gtcggcgtga
     7261 ggcggctctc ctacgagttc ggcggggcgg agaagggcga gatcgagctc acgccgggga
     7321 acccctactt cgtgaaggag ctcgactccg gcgccctgaa gggcgacaag gccgagatcg
     7381 tcctcctcgc cgaggacagg atcggcaacg tcacgaggct cgcgctcgcg cccaaggtgg
     7441 accggaaggc cgacaagccg gtcgtccgcg tctcctaccc ggcctccggc ggccaggcca
     7501 ggccgggcga gtccgtctgg ggcgcgatag ccgacgacga tggcgcggcc atggtgcgct
     7561 tctccttcga cggcggggcg ccttccgagc tgcccgcctc ggagcatttc gccatccccc
     7621 ttcccgagct cgcctcgggc aggcatgtcg tctccatcac cgctgtcgac gtcgagggcg
     7681 cggcgggcga tcccctcgtc ctgcccttcg tcatggaccg cggcccgggc gccgtctcct
     7741 tccttcgcgt ctcgggcggc gcggcgaagc aggccgccgg caaagcggct ccggccgagg
     7801 gggccaggga ctacgcgagc ggcgtcgagg tggccctgga ctcggggagc ctcctcgagg
     7861 gccgcgtcga gtcggccaat ccgcccctct ccgccgagtg gtcggtcgca ggcggcgcgc
     7921 cgcggaagct cgagctttcg aaggccgcgg agcccggcgc ctattccttc aggctgcccc
     7981 tggaccggag cctgccctac ggcttcgcgc cgatcgaggt gaaggtcgcc gacggcttcg
     8041 gcaactccta ttcgggccga gccctggtct acgcgaccaa cctgggcgcg atccgcgaag
     8101 actcgggctt ccgcttcgac gacccgagga taggcgaggg cgggcgggtc gtcctctccg
     8161 ccgcggagcc gctcctgggc gccttctacc gcgaggagct cgcctccctc cgcctggagc
     8221 ccgcgacgga catcgtcgcc gcgagcttcg acggccgcgt cgtctccctg gccgtcctca
     8281 aggagggcgc gacgcctccc ctgcgcctgg tcgggaagac gaagcgcggc cacgacttcg
     8341 aggcggggcc cttctccttc gcctgcgact ccattcctcc gcgcatcgag atcgagtcgc
     8401 ccgcggaagg ggcctggcac tcgggccgct tcgaggcgaa gggccgcgtc gtcgactcgg
     8461 gcggcctcgc gaagctcctt gtccgttcct ctcccggcgc cgaggcccgc gagctcaagg
     8521 tcgggtccga tggacgcttc tccttcttcg tcgagcccgc agcttcggcc gccgcggccg
     8581 gagggatcgc ccccgcggaa gccggctcgg ccgggccctc ctacctcgag gtcgaggccg
     8641 tggactcgtc gggcaacgcg gcccgcgcct acaggagcta cggcgtcgac gcgaaggctc
     8701 ccgcactgcg cttcctctcg cccgaggccg gcgcccaggt gagcggcccc gaggacatcg
     8761 ccgcggtcgc cgaggacgcc tccggcctcg cctcggtcga gtacgccgcc gacggagcct
     8821 ccttcactcc ggtcgagcgg cgcgggaacg ccttcatcca ccgcgccgat ctcgcggcca
     8881 atcccaaggc cgcctatcgc gtcaccgacc gggccggcaa cgccgccgtc ctgcggcccg
     8941 aagtgaaggc cgtcccggcg gccaagcccc tccccgccgc cgactcggtc tccgtcgagc
     9001 ctgaggccgg cgaggcgcgg ctcgagctcg cgggcgcggc cggaacgggc gccgccgcgc
     9061 ggaagctctc ccttctcctc cccgcccttt ccgaggaggc cttcagcgcc ctcggcctcg
     9121 cggaggggaa ggaggcgccc gagcgcttct ccgtccgcct cctcgcgtcc ggcgccctgt
     9181 ccctcaaggg caaggtcgcc tccgagggca aagtgaaggc cctctccttg agcctggacg
     9241 gcggcgctag ctgggcgccc ctcttccagg ccaaggacgc caagtcgatc ctccccgagg
     9301 cggcggtggc gctcaacgtg gacacggcga agctccccga cggggaggcc cgcctcgtcc
     9361 tcaaggtgga gggcgaggcc ggcggcctct cctacgtccc gctctatctc ctcgtcgaca
     9421 acagggcgcc cgaagccgcg attctcgcgc ccgaggcctc agtcgccgcc ctcccgggcc
     9481 cctatcccct ggtgctgaag atctcggatc cgcccggacc ttcgagtggc gcgggtgtca
     9541 ctgcactgac gtctggcggc ggggccttca gcggggtggc cgcggccgag ctcagcctgg
     9601 gcgcggacaa gaaggccctc cccgtggcgg agggcggctc ctactacgca ttctgggccg
     9661 atcccgaggc cgccgcggcc gccgccaagg gcgccgccct cgctgtctgg ctctccgccc
     9721 gcgacgccgc cggcaacgcg gccgccgcga caaggaagct cgcctacgac gccgccgccg
     9781 acgcgccgcg gattactctc tccctccccg cgccggcggc ggtcgccgcc cccgccccgg
     9841 gcgccaagcc ggccccggcc gcgaccgagc ggctcgtcct ctcgccaggg gacgcgatag
     9901 ccggactagc cgcggacgac gacggcccgg cggagctccg cctctccttc gacgggggcg
     9961 aggccctcgt cttccccgcc ggagccttcg ccctcgccct gccggccctt tcgcccggca
    10021 ggcattccct gaagctcgag gcccgggacc agggcggcaa gtccgcggag gcgaagcgcg
    10081 agctcctcgt gcgcgggccc gcgccttcgt tctccggcgt cgaggccggg gccgcctcgg
    10141 gccgcaaggc ctggctgccc ggcctggagc tcgccctggc gtcgggttcc gcgctgtcgg
    10201 gccgcgtcga ggcgcccaac ggcctctcct ccctcgagta ctcggtgaac ggcggcgcgg
    10261 cgcagaaggc cctcctcggc aaggccgagg cgggcggcgc cgcggcagcc gcgctgccct
    10321 tcagcgccgc cctccctgcc tcgctcccct acgagcgctt cgtcgtcgaa ctcgtggccc
    10381 gcgacctttc gggcctggag acgaggcggc gctacgactt ccacgccgtc ctgccctccc
    10441 ttcccggcgc cccgccggcc gacgacgcgg aaggcctccg cttccacgac gcccgcgtct
    10501 cggccgaggg cgaggcgctc cgcctcctcc tcaagccggg cgagcgcctc tccgggcgct
    10561 tcaacggcag gccgcttgcg tccctcgccg tcgagccctc cgtcggcttc ctctccgcct
    10621 ccttcgaggg cgcctccgtg tccctggaag ccaaggctga gggcctctcc ggccctccct
    10681 acgccgcggc ccagggtgcc ttgcgcctcg tcgccgtcac ggtggacggc gaccgcttcg
    10741 agtgggggcc cttctccctc gccgtggacg cggcctctcc ctccctcgcg ctggattcgc
    10801 cggccgacgc ctcctggacc aagggtccga cgcggatcgc gggccgggcc gaggatccga
    10861 acggcgtcgt cgccgtggag ctctccgtga acggaggccc gcggacgcgc ctcgagcccc
    10921 gggccgccgc gcccgccgcg acgccgccag ccgctcctgc cgcgcccgcg gcttccgcgg
    10981 ccaaggcacc ggcggccaag ccggccgccg ccccggcccc cgcgccctcc gcgtcagccg
    11041 cccctgcgcc caagggcccc ctggccgcct acaccttcga cgccgagctg cccctcgccg
    11101 aggcggagga cggcatcatc cgcctggagc tcttcgcgcg ggactcggcg ggccgcgagt
    11161 cgagcctgac gcgcttcgtc tacaaggaca gccagccgcc cacgctctcc ctcgtcctcc
    11221 cgccgcccgg ggaggcggtc aacggcacga cgaccctcgt cggcgaggcc gccgattccg
    11281 gccgcctcgc ctcggcctcc ttctccgcgg ccaagggggc cgcggccgag gaggtcctgg
    11341 gcctctcggt cttctcgcgg acggtggacc ttgcccgcgt cgccttcccc ctccccgagg
    11401 gctacggctt cgcggtcgcc gaccgggcgg gcaacgtcgc cgtcctctct cccgagctcc
    11461 tcgtcgacaa ggagaaggac aagcccgtgg tggagatcgc cgccccggcc gagctcgagg
    11521 tgatccgcgc cgacttcttc gtctcgggca cggccttcga cgacgacggc gtggccgcga
    11581 tcgagtaccg cctggacggc ggcgaatggg tccgcgtcga gcccaagggc gcgggcttct
    11641 ccgtgcccag gaccctcgcg gagacggcgg acaacgagca cctcgtggag gccttcgcca
    11701 aggacctcta cggcgtcaag ggcgacacgg tcgcgcggcg cttccgagtc tccaaggagg
    11761 agcccctcgc gcgcatgacc tctccgagca tcgccaagcc ccagcgcggc ctcgtggagc
    11821 tcgcggggga ggcctccgac gccaacggcg tcgcctccgt ccgcctctcc ttcgacaacg
    11881 cgacggcctt caacctgggc tccggggccg agtcctggcg ctatcccctc gacacccggg
    11941 tcctggccga cggcctccac gccatagcgg cgaagcccct ggacaagtac gacacggagg
    12001 gcttctacgc gaccctcctc aacgtggaca acacgccgcc ccgcgcggag ctctccctcc
    12061 cggccgacgg agcctcctac tccgggacac ttccgctgag cggaagggtg tcggacaacg
    12121 tcaggctcga ctcggcccgc gtcgaggtcg cgcccatcgg gcagggcagg ccgcccgtcc
    12181 tcgtcgtgga cctgggacag gcggccgtcg tgcgcaggga gctcgacgtc tccgccctgc
    12241 ctccgggcgg ctacacggtg cgcctggtcg ccaaggaccg ggccgacaac gagaccctgg
    12301 cgagccgcga cgtcgtcctc aaggagggcc gtccggcgga catggtggag atcctcttcc
    12361 ccgtggaggg cgaggagctc tcgggcgccg cccggctcta cgggcgggca gccgtctcag
    12421 gcggcgtaag cgccgcgacg atcctcgtgg acggcgccga cgtcggcacg gccgccgtgg
    12481 acgcgcgggg ctggttcgcc ttcgaccttc cggccgacaa gctggaggag ggcacgcggc
    12541 ggatcgtcgc cagggcggcc gcttccgacg ggcgcctggt cgagtcccgc gcggagaccg
    12601 tgtcgtggaa gcgcctgggg ccctgggtct ccgtcgactc cctgcccgcc ggcgcctacc
    12661 tgccctacag gccctatctc tccggcggag cgggctacct ggccgccgcg ccggcggctg
    12721 gcgacaaggc cgcccaggcc gccttcaaga agacggaagg ccagcgccgc gtgacgcgcg
    12781 tcgagctcag cctggacaac gggaggacct tcctgcccgc gaagggcggc gagcgctgga
    12841 agttccgcct ggaaacccag gactaccccg aggggagcct ccacgtcatc gtgcgcgcga
    12901 gcttcggcga cggttcgacc gcggtcgcga agaccatcct cgccctggac aagacgccgc
    12961 ccgaggtggc cctggagcgg ccgcaggaga acggccgcta caacgcggag atccgcgccg
    13021 cgggcgtcgc ctccgacgag tcgggcctgg agagcgtggg cctggccctg cgcaagggcg
    13081 acaagcgcgg ctacgagctg cccgccttca tccagggcct ctacatcgac ggccacttcc
    13141 tgggcgcgac ctactacgac atcggggcgg gcctctcctt cttccaggac aacgtcaagc
    13201 tccaggtgat gacgggcatg gcgccggagg agggacgctt cggcggcagc gtcatgggcg
    13261 cgaagctcct cgcgaacatc gcctaccttc ccgcgaattt cctcttgggg cccgactgga
    13321 acttcctctc ggcctcgctg gcgctgggcg ccaacttctc ctacttcagc cagtcggcga
    13381 gcggctcggg cctcatcctg ggcgccgtcg tggcccagct ggagttcccg aagatcacgg
    13441 tccaggactg gagcttcctc aagaaatact ccttctacac ggagggccaa gcctggttcg
    13501 tctcctccga cgtccagggc ggcatcctct tccgcctgtc cttcggcgcg aggctcggcc
    13561 tgttctaggc gggcttgctt cgcgttacgc ggaaaaaaag aagagcgcgc cccggaaggg
    13621 acgcgctctt cttgtatgga cggattgcgt cggagttgcg gacgttgagc gctgcgggcg
    13681 gcccgggcgg cgggcagcgc gcagcggcgg catgcgtcgc atcggcctga gccgcgatag
    13741 gcggcaccgc gggtccaatc cgcggagccc ggcggcccgg gcctcctagt ggatccaggg
    13801 cctgagcgcg tccctcgcga tcaggaagcc ccgcttcacg agctcctcgc ttccctcgaa
    13861 gttccggtcc tcgtgctcca ccgacacgac gtagtcgtag cccgagcggt agagggcggc
    13921 gacgaagcgc cgccagtcca tgtcgcccag gcccggaagg cgcggtacgg cccagcccat
    13981 cccctgggag aggacgccgt tccggtagag accctcgcgg tcgatcatga ggtccttcgc
    14041 gtggacgtgg aagatgcggg agccgaagtc gcggacgacg cgctcgcagt ctatcatctg
    14101 ccagacgagg tgggaggggt cgaggttcag gccgaagttc tcgtcgggga tgatttccca
    14161 catgcgcgtc cagaccgccg gcgagaccgc gaggttcttt cccgccggcc attcgtcggc
    14221 gctgaagagc atggggcagt tctcgatgcc gatcttcacg ccccgctcgg ccgcgtagcg
    14281 gacgatggga ggccaggtcc tggcgaaggc ctcgaggttc tcgtcgaggc ccttcctcca
    14341 gtcgcgcccg gtgaaggtgc cgacgacggg gacttcgagg cgggcggccg cgtcgatgac
    14401 cttcttgagg tgctcgatcg cgggtcccga cacggcgggg tcgggatcga gcgggttcgg
    14461 gtagtagccc aggctggaga tcccgaggcc gcgggaggcc agcgccttcc ggacggccgc
    14521 cgcgccggcc ttgtccaggc cggccgcgtc gacgtggctc acgcccgcgt agcggcgctc
    14581 ggcccgggag acgggccagg cggcgagctc cagggcctcg aagcccgagg ccgccgccca
    14641 gtccgcgagg ggctcgaggg cgaggccggg gaaggccgcg gtgaagagtc cgagcctcat
    14701 gcctggcctc ctttccgcct gggcagctcc acccaggcgc ccttcctcga gctctcgagg
    14761 agggcgtcgc agaggagcat ctcctcgtgg ccgtcgtcga aggtcgggaa gtcgcggggc
    14821 gcggcgtagt cgccggcctc gacgtaggcg tagaaggagg cgaacatctg cttgagggtg
    14881 tccgcgtagc cttcctggtg gccgcccggg taggagacga gccgcctcgc ggcgggcgtg
    14941 aggagggcgg gatccttcat gagaaggcgg ttgggctcgt cgcgcgcgcc gagccatagg
    15001 gcgttcggct cctcggagtc ccaggccagg gaggaggcgg agccgtcgat ctcgaagtgg
    15061 agcgagttct tccggccggc ggagacctgg gagacgacga gggagccgcg gaggcccccc
    15121 tcgaagcgga ggaggatcgt cgcggcgtct tcgctcgcga cggccaccgg tacgagctcg
    15181 cccgaggggg cgccggtctt gccggagaag gtctccacgg ggccgcgcgg ccggcgccgg
    15241 acggggtgga aggtggcgaa gtcggccagg accgcctcga tcctgcggcc cgtcgcgtag
    15301 gcagccatgt ccagccagtg cgtgcctatg tcggccaccg cgcggagggc tccgcccgcc
    15361 tccggctcga ggcgccagtt ccagtccgtg tcgaggagga gccagtcctg gaggtaggcg
    15421 ccgtggacga ggaagggcga gcccaggtcg ccgcgggcca cccgctcccg ggcctcgcgc
    15481 accagggggt agaagcgcag gttgtagttg accgcggcga cgccggggga gcgccgggcg
    15541 agctcggcga ggcgcgcgga ctcggcgctc gacatggcca gcggcttctc gcagatgacg
    15601 tgcttgccgg ccgcgagggc ggcctcggcg tagggcgcgt ggagggcgtt gggcacggcg
    15661 aggtggacga cggacaccgc ggggtcggcc agggccgcct cgtaggaggg ataggcgcgc
    15721 tcgatgccga gctcgcgggc cttgcgcgcg
//
LOCUS       DAOOSJ010000026        15158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2661_length_15158_cov_7.160961, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000026 DAOOSJ010000000
VERSION     DAOOSJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 15158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15158
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_15158_cov_7.160961"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..901)
                     /locus_tag="PLG14_02525"
     CDS             complement(182..901)
                     /locus_tag="PLG14_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48036.1"
                     /translation="MRLYDRLIEESLALLEGLPGRRLAAPGEAWPEAGEGALILRSES
                     AYELGGGSLAAASAFGLSSSEALVGGDEVWLYGPDLPDIKEDAPYARLAFVRVAEGGL
                     GEGEAAYAAIRGIEQTRYRVSPRGYMARISAASEREPVRVGRAALREGLDFAKVGGHY
                     LKAYRARPQVLAAKLVFVTLPGFPYGELARLASRAEAITESLDHVLKDLVMDCASCHL
                     KPVCDQVEGMRELHFSRGKRP"
     gene            complement(904..1059)
                     /locus_tag="PLG14_02530"
     CDS             complement(904..1059)
                     /locus_tag="PLG14_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48037.1"
                     /translation="MKAVIIVAGVALALFLLSLVVYFLNLDMKAAARLQPLFLKRYGK
                     LKRDRRL"
     gene            complement(1070..1873)
                     /locus_tag="PLG14_02535"
     CDS             complement(1070..1873)
                     /locus_tag="PLG14_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOX48038.1"
                     /translation="MNYFAGCDAGSTYTKCVIVDEKGAMVSSVTSRSRINPVLSAQAA
                     LDEAVAKVPGLKDAKGLAYLVGTGYGRNKVPFADENISEISCHAMGVHVVDPEVKSIL
                     DIGGQDVKGISVDRDGTVLGFAMNDKCSAGTGRFFEAMAHAFEMPLSDFSKLSLKARN
                     VIPITAQCTVFAESEVISLVGEGKPMDEIAAGIQLSVAKRCFVMAKKAAATDSIALTG
                     GCAKNDGLKQALEKVLKLKVVDLPVDPQLMGALGAAEYARQKGLATAKA"
     gene            complement(1901..3427)
                     /locus_tag="PLG14_02540"
     CDS             complement(1901..3427)
                     /locus_tag="PLG14_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOX48039.1"
                     /translation="MGSERIGKVYKGGKTRARREWRGLRDTWYDYTRWLFIWKTMIGF
                     MMNLNNIRGFFRYRWMANYLAVPDFLDRHTEGLRGPQLRIAHTEFDLIVEHMCDTMTT
                     MFKADPLIGNDKKLSASIVVFDENMMSQIMNGFPNLHMVCIQIPPVYTSSTMTQEGVI
                     HYIDVAQEFGIPADVCPMPAAELGVAIDDDYPHIGTCAVQCNTTCDGSLMGNGLEARR
                     YGIPTFQLPVPIRHTQDSVQDYAAEEIMNAIRFIEEQTGEKFDWDNYWKCMEVFNEET
                     KQMLEWLELSRSPYPQVIGNNVALYRDATYQVAGGRDARFLDADREITRLGMEGYASK
                     SLCAPVVRHRAILWGVQAQYYTAFPLWLQNCWGILPLIDMLSLTSTRIFQIGDERQAL
                     LDMAHLYQNMTMRNRSNGGYEVGVKDLWRFCESFKADMVIMYEHIGCKAMAGYHGIFE
                     EQARERGIHLVWVTHGLMDPRAASRKDMRGEVSRYMRAVMGEAPLDPSLEDFDDSNAW
                     "
     gene            complement(3436..4629)
                     /locus_tag="PLG14_02545"
     CDS             complement(3436..4629)
                     /locus_tag="PLG14_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOX48040.1"
                     /translation="MKDLKHLYYFENLLQEANNDLVRQAASEGRVAVGSVCSLVPEVL
                     LSLPGCFGVRLRAPRTGSMEMGTYYMTSLLCECCRAILERAIEGGYKFLDCVIAPDAC
                     AQMNRCVENIDLQKLCDKEKFFVSYADVPMKSGDAALRHYVKQMRLRALDPLRDTFGI
                     DTSDAALREAVARHNEVCRLITAIGDLRKEERPRITGYEFHVLCTATYCCPKDLLVEK
                     LRETLGELKAREPAPEGAYRARVVMAGSELDSPDLVKLAEDSGALVVADRFCFGSLPG
                     RQEIVLTEGEDVLTQICRQYLEQGQCPRYMDTAKINERKAYVDALAKEYRADGIVYEQ
                     MKFCDYWGYERAAASRIMREEFGYPVLSVDVPYVVGTNAQLRTRMQAFVESVEIKKIQ
                     GEAKR"
     gene            complement(4641..5432)
                     /locus_tag="PLG14_02550"
     CDS             complement(4641..5432)
                     /locus_tag="PLG14_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX48041.1"
                     /translation="MVSGTGTAEGVTKIIAVAGKGGVGKTSLAAAIVKSLVEAFPGKR
                     IIAIDADPAVGLSTALGIEVETTIDDIRKAVVASVEDGDTKEAVELLGEARYRIYDAL
                     VEAEGFAFIAVGRPEAEGCYCRINSYLKEVIGILSKNFDYVVVDGEAGIEQVNRRVME
                     KVTHLLLVTDASRKGAQVVRAIRKVADELVMYEKVGAVANRLPDAAAADLLDTGGVPL
                     VARVGCDPALAAFDLEGKSVLALPGDSEITRGAREALRNIGILKG"
     gene            complement(5697..6212)
                     /locus_tag="PLG14_02555"
     CDS             complement(5697..6212)
                     /locus_tag="PLG14_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOX48042.1"
                     /translation="MKGKFLASAALALLVLSSCAGGPEEAGEGQAEGSPGGDLVAVFD
                     FELKGGGPEYESLRTDAPEAVAEALLRGGVLRPVERRQLEKALAEQALSLSGVVDEAT
                     AARAGRVAGARFVLLGSVSFVAGQARVSCRVIDAETAELVYAQSAYGDAEYIFDLVDE
                     LAGMVEDEFSE"
     gene            complement(6423..7295)
                     /locus_tag="PLG14_02560"
     CDS             complement(6423..7295)
                     /locus_tag="PLG14_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX48043.1"
                     /translation="MPEWGMALLLAGLSALAYGIADFSGGYAARKSSVLSVVALSQIL
                     GLALALSFIAASGTPLPAPRDLLWGLAAGLAGSAGLLLLYRGLATSLVAIVSPSSALV
                     GALVPMAFGLALGERPSGPALAGAALCLPAVILLGSGSGDGQGADKAAARRESRRALA
                     FGVLAGLGFGGFFVAAARTSPASGLWPLVAARTASIGVALSALLASGSRPRLEKGDGP
                     ATAVAGLADMGANILFLLAARSGLLTLVAVVTALYPAPTVLLGRIFLKEKVTLARAAG
                     LVLAIVGVALLSLR"
     gene            complement(7313..7768)
                     /locus_tag="PLG14_02565"
     CDS             complement(7313..7768)
                     /locus_tag="PLG14_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOX48044.1"
                     /translation="MIPEKVAKVLAAAGLEALEFEPGSTPTAPLAAARIGVEVGRIAK
                     SLLFRAKDGSYHLVVCAGDRRVNSGRLKALVGSKASMADAEETLRVTGYPPGGVCPFA
                     LEGVAVHLDESLAAYETVYPAAGTDSTGVPVSFERLLAVTGGSRVAVTE"
     gene            complement(8003..9040)
                     /locus_tag="PLG14_02570"
     CDS             complement(8003..9040)
                     /locus_tag="PLG14_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX48045.1"
                     /translation="MFKPEEIIFAPTARCNLACAHCRVARVPEELAASDAVAFLREAA
                     ERGVGRLGFSGGEPFLRPDFLRELCAEAVSLGLLFDRLMTNGSWWRDEAELEETLGSV
                     AEAGFDGTIGLSADRWHGSTAGRLLPFIEAAHRAFGRRDCVEIASVVSPDDGPLLDIF
                     RAVAAALGGELVLSGGEPEAIVPAKGAGRRGARAAAEGEELRVDIVRIPYSAAGEPGA
                     ADGAAAGGQAAGAGGTPASGPWSAKEWFTDDFCAGPGNVLYVHPNGKVAVCCGFANEN
                     EELVVGRIPGDGVDAVLARAAASPRVRACYETGLGEVRKRLEAEGFAFPGKTADICFF
                     CDFLCKRGLGA"
     assembly_gap    9267..9366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9394..10977)
                     /locus_tag="PLG14_02575"
     CDS             complement(9394..10977)
                     /locus_tag="PLG14_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48046.1"
                     /translation="MRIRARLALSFLSVIAALSAAALLMVGPAAESAFRSFVFSGDAE
                     KARVYGSLLADFYDEKGNWEGAQAFVAGMPARLLASIEESVHARRAAPEASTAALADA
                     AAYAGAAKAPTAGQGLSAGAEAAAAAESAYALLSDRVALADPDGVVVVDTAGRLLGSV
                     HPPAHLAHGLPVLSGGRRVGTVLVGSMIESSLTAPGERFLRRVFSAVLSATLLSAALA
                     LLLGLGLSARMTRPLAALARAARRVQAGDLSARVDARGGDEVAELSGSFNAMAAELAR
                     LEEAKRRIIADAAHELRTPAALIQGAVEAMLDGVFPRDDETLRSVHEEALRLARLIES
                     LRELESAESGELELRIEELDALEEARGAAALFEAPARAKGLSLCVSGEGAARVLADAF
                     RLREILSNFLANAVRHAPEGGRVRLLARERQAPDPPARRDRDGGWRTAAPFVEFAVED
                     SGPGVPPAERERVFERFYRLDASRSSGTGGRGLGLSIASGLAKAMGGRIELGESELGG
                     AAFLLLLPAAGNGVPPPGP"
     gene            complement(10967..11668)
                     /locus_tag="PLG14_02580"
     CDS             complement(10967..11668)
                     /locus_tag="PLG14_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX48047.1"
                     /translation="MARGLVLVADDEEKIARMVGSYLEASGFEAALAFDGLAALAAFR
                     ERKPDLAVLDISMPGLDGLELAREIRRSSDLPVIFLTARAEEADRVLGLELGADDYVT
                     KPFSPRELVARVRAVLRRGRREGAAPEEVLRRGSLEMDFGRRSVRVDGEPKALTAVQF
                     DILAFLAREPGRVRSRLSILESASGEAFEGYERTVDAHVKNIRKALGDDSAAPRFVET
                     VRGVGYRFMEPRDAD"
     gene            complement(11684..12406)
                     /locus_tag="PLG14_02585"
     CDS             complement(11684..12406)
                     /locus_tag="PLG14_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48048.1"
                     /translation="MKIHGIRSAAVLTALAAAAALAGCAATDTVGKFAASSFASLAEA
                     SRDRISYEAEDGAWALASPAGDLFLLSSDFARNAGSGGMADMDRPDAELSLDAAPFLA
                     AGLDVAKIPAAEGIKYELEDGRFMLHFELGKAAFAPAAKDSMTAAFGELLKAERARVS
                     YHAALDHYGIMLGGGNMVEWAKDLSKNDKDLVFVLEPGPLVAAGLDPAKVEGWVFAKV
                     ETMDAKGKKLLVDKLLRPFELR"
     gene            complement(12510..12725)
                     /locus_tag="PLG14_02590"
     CDS             complement(12510..12725)
                     /locus_tag="PLG14_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOX48049.1"
                     /translation="MRKLLTIEGMSCGHCVMHVKTALSEVPGVAKVDVDLVKKSAMVE
                     GEALSDAALKAAVAEAGYSVSEVLGGR"
     gene            complement(12738..>15158)
                     /locus_tag="PLG14_02595"
     CDS             complement(12738..>15158)
                     /locus_tag="PLG14_02595"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX48050.1"
                     /translation="ACATASERAVRKLPGVAEASVNFATERLSVSWDDAALSLDAVKA
                     AVAKAGYEAVDDVEAREISLPIGGMSCASCAAAVERAIGKLPGVSAASVNYATEKARV
                     SYDPARLRLSEIRAAVVKAGYEPLALADGGEKVDEHKAEKEREVRGLWRRFTVSAVFS
                     APLLYIAMGSMFGWPLPAGLSMMAYPLRLALVQLFLVVPVVAAGRRFYSVGFKALFRG
                     APNMDSLIALGTSAAVLYSLYSAARIAAGDFRAVESLYFETAGVIITLILLGKSLEAV
                     AKGRTSESIKKLMGLRPKTATVVHGETELEVPVDEVEAGDLVRVRPGEKIPVDGLVVS
                     GRGAVDESMLTGESIPVEKEAGDRVIGASLNKNGSLVFRATEVGADTVLSRIVRLVEE
                     AQGSKAPIARLADVVSGWFVPIVLGIALLSAVAWLLLGHTPVFALTVFVAVLTIACPC
                     ALGLATPTAIMVGTGRGAELGVLIKSGPALETAHRVDTVVFDKTGTITKGSPELTDLV
                     PAPGFGGDELLSLAAAAEKASEHPLGEALVRAAEARGLALPPAESFSALPGRGIEAKV
                     GGREVLAGSARLLAERGIGSGDGEAKAASLAEDGKTPMFVAVDGRYAGLVAAADVVKE
                     SSAPAIAALHAMGVEVAMITGDSARTAAAIARRVGIDRVLAEVLPEDKAAEVKKLQAA
                     GRRVAMVGDGVNDAPALAQADVGIAVGSGADVAMESADFVLMRSDLMDVPTAIKLSRS
                     VIRNIKQNLFWAFGYNVLGIPVAAGLLYAFGGPLLNPIFAAAAMSMSSVSVLTNALRL
                     KRFKPYRV"
BASE COUNT         2126 a         5094 c         5654 g         2184 t
ORIGIN      
        1 caggatagcc ttcaagccgg tcgcgaagct cttcgcgaag ggcgccgagt agggaacggc
       61 cgccggccgc taaggccggg ataaaagaaa gagccaggcg cgcagcctgg ctcttttctt
      121 ttcttgcggg cggaggcggc agcgccgccg gcggcgcgat cagcgtgtca gggcagcgcc
      181 gtcagggccg cttaccccgc gagaagtgga gctcgcgcat cccctcgacc tggtcgcaga
      241 cgggcttcag gtggcaggag gcgcagtcca tcacgaggtc cttgaggacg tggtccagcg
      301 actcggtgat cgcctccgcc ctggaggcca gcctcgcgag ctcgccgtag gggaagcccg
      361 gcagggtgac gaagacgagc ttggcggcca ggacctgggg ccgggcgcgg taggccttga
      421 ggtagtggcc gccgaccttc gcgaagtcga ggccctcgcg gagggccgcc ctgcccacgc
      481 gcacgggctc gcgctcgctc gcggcggaga tccgcgccat gtagccccgg gggctcacgc
      541 ggtagcgcgt ctgctcgatg ccgcggatcg cggcgtaggc cgcctcgccc tcgcccaggc
      601 cgccttcggc cactcgcacg aaggcgagcc tggcgtaggg cgcgtcctcc ttgatgtcgg
      661 gcaggtcggg gccgtagagc cagacctcgt ccccgcccac gagggcttcg ctggagctga
      721 ggccgaaggc gctggcggcg gcgaggctgc cgccgcccag ctcgtaggcg ctctcgctcc
      781 ggaggatgag ggcgccttcc cccgcctcgg gccaggcctc gccgggcgcc gcgaggcggc
      841 gtcccgggag gccttcgagg agggcaaggc tctcctcgat gagcctgtcg tagagccgca
      901 tccctagagc cggcggtccc gcttgagctt cccgtagcgc ttgaggaaga ggggctggag
      961 cctggcggcc gccttcatgt ccaggttgag gaagtagacc acgagggaga ggaggaagag
     1021 ggccagcgcg acgccggcga cgatgatgac cgctttcatg ctattcccct tatgccttcg
     1081 ccgtggccag gcctttttgc ctggcgtact cggccgcgcc gagggcgccc atgagctggg
     1141 ggtcgacggg caggtccacg accttcagct tgagcacctt ctccagggcc tgcttgagtc
     1201 cgtcgttctt ggcgcagcct cccgtgaggg cgatgctgtc ggtggcggcg gccttcttcg
     1261 ccatgacgaa gcagcgcttg gccacggaga gctggatgcc cgcggcgatc tcgtccatgg
     1321 gcttgccctc gccgaccagg gagatgacct cgctctccgc gaagacggtg cactgggccg
     1381 tgatggggat cacgttcctg gccttgaggg agagcttgga gaagtcggag aggggcatct
     1441 cgaaggcgtg ggccatggcc tcgaagaagc ggccggtgcc cgcggagcac ttgtcgttca
     1501 tcgcgaagcc gagcaccgtg ccgtccctgt ccacggagat gcccttcacg tcctggccgc
     1561 cgatgtccag gatggacttg acctcgggat cgaccacgtg cacgcccatg gcgtggcagc
     1621 tgatctccga gatgttctcg tccgcgaagg gcaccttgtt gcggccgtag cccgtgccga
     1681 cgaggtaggc gaggcccttg gcgtccttga ggccgggcac cttggcgacg gcctcgtcca
     1741 gggcggcctg ggcgctcagg acgggattga tgcggctgcg gctcgtcacc gaggagacca
     1801 tcgccccctt ctcgtccacg atcacgcatt tggtgtaggt gcttccggcg tcgcagccgg
     1861 cgaagtagtt catgtcgtcg ttctccttcc gtcgtcccgc ctaccaggcg ttagagtcgt
     1921 cgaagtcctc gaggctcggg tccagcggag cctcgcccat gacggcgcgc atgtagcggc
     1981 tcacctctcc gcgcatgtcc ttgcgggagg ccgcgcgcgg gtccatgagg ccgtgggtga
     2041 cccagaccag gtggatgccc cgctcgcggg cctgctcctc gaagatgccg tggtagccgg
     2101 ccatggcctt gcagccgatg tgctcgtaca tgatgaccat gtcggccttg aagctctcgc
     2161 agaagcgcca caggtccttc acgcctacct cgtagccgcc gttggagcgg ttgcgcatgg
     2221 tcatgttctg gtagaggtgg gccatgtcca ggagggcctg cctctcgtcg ccgatctgga
     2281 agatccgcgt ggaggtgagg ctgagcatgt cgatgagggg caggatgccc cagcagttct
     2341 gcagccagag ggggaaggcc gtgtagtact gggcctggac gccccagagg atggcgcggt
     2401 ggcggacgac gggggcgcag aggctcttgc tcgcgtagcc ttccatgccc agccgcgtga
     2461 tctcccggtc ggcgtccagg aagcgggcgt cgcggccgcc cgcgacctgg taggtggcgt
     2521 cgcggtagag ggccacgttg ttgccgatga cctgcggata gggcgagcgg ctgagctcca
     2581 gccactcgag catctgcttg gtctcctcgt tgaagacctc catgcacttc cagtagttgt
     2641 cccagtcgaa cttctcgccg gtctgctcct cgatgaagcg gatggcgttc atgatctcct
     2701 ccgcggcgta gtcctggacg gagtcctggg tgtggcggat gggcacgggg agctggaagg
     2761 tcggaatccc gtagcgcctc gcctcaaggc cgttgcccat gaggctgccg tcgcaggtgg
     2821 tgttgcactg gacggcgcac gtgccgatgt gggggtagtc gtcgtcgatg gccacgccga
     2881 gctcggcggc gggcatcggg cagacgtccg ccggtatgcc gaactcctgg gccacgtcga
     2941 tgtagtggat gacgccttcc tgcgtcatgg tggagctcgt gtagacgggc gggatctgga
     3001 tgcagaccat gtgcaggttc gggaagccgt tcatgatctg gctcatcatg ttctcgtcga
     3061 acacgacgat gctggcggag agcttcttgt cgttcccgat gagggggtcg gccttgaaca
     3121 tcgtggtcat ggtgtcgcac atgtgctcca cgatgaggtc gaactccgtg tgggcgatgc
     3181 gcagctgcgg cccgcgcagg ccctcggtgt ggcggtccag gaagtcgggc accgcgagat
     3241 agttcgccat ccagcggtag cggaagaagc cgcggatgtt gttgaggttc atcatgaagc
     3301 cgatcatggt cttccagatg aagagccagc gggtgtagtc gtaccaggtg tccctgaggc
     3361 cgcgccactc gcgtcgcgcg cgggtcttcc cgcccttgta gaccttgcct atgcgttcgc
     3421 ttcccatcgc cttgctcatc gcttcgcctc gccctggatc ttcttgattt ccacgctctc
     3481 gacgaaggcc tgcatgcggg tgcgcagctg ggcgttcgtc cccaccacgt agggcacgtc
     3541 cacggagagg acgggatagc cgaattcctc gcgcatgatg cggctggccg cggcccgctc
     3601 gtagccccag tagtcgcaga acttcatctg ctcgtagacg atgccgtcgg cgcggtactc
     3661 cttggcgagg gcgtccacgt aggccttccg ctcgtttatc ttcgcggtgt ccatgtagcg
     3721 ggggcactgg ccctgctcca ggtactgccg gcagatctgc gtgaggacgt cctcgccctc
     3781 cgtgaggact atctcctgcc gtccgggcag ggagccgaag cagaagcggt ccgccacgac
     3841 cagggcgccc gagtcctcag cgagcttgac gaggtcgggg ctgtcgagct ccgagcccgc
     3901 catgaccacg cgcgcccggt aggcgccttc gggcgcgggc tcgcgggcct tgagctcccc
     3961 cagggtctcg cggagcttct ccacgaggag gtccttcggg cagcagtagg tcgcggtgca
     4021 caggacgtgg aactcgtagc ccgtgatccg gggcctctct tccttccgga ggtcgccgat
     4081 ggcggttatg aggcggcaga cttcgttgtg ccgcgcgacg gcctcgcgca gggcggcgtc
     4141 ggaggtatcg atgccgaacg tgtcgcgcag gggatccagg gcccggaggc gcatctgctt
     4201 gacgtagtgc cgcaaggccg cgtcgccgga cttcatcggc acgtccgcgt aggagacgaa
     4261 gaacttctcc ttgtcgcaga gcttctgcag gtcgatgttc tccacgcagc ggttcatctg
     4321 ggcgcaggcg tccggcgcga tgacgcagtc caggaacttg tagccgccct cgatggcgcg
     4381 ctccaggatg gcgcggcagc actcgcagag gaggctggtc atgtaatagg tgcccatctc
     4441 catggagccg gtgcgcggcg cgcgcaggcg gacgccgaag cagcccggga gggagaggag
     4501 gacctcgggg acgaggctgc agaccgagcc cacggcgacg cgcccctcgg aggcggcctg
     4561 gcgcacgagg tcgttgttgg cctcctgcag gaggttctcg aagtagtaca ggtgcttaag
     4621 gtctttcatc tctcgcaaac ctatcctttc agtatgccta tgttgcggag ggcctcgcgc
     4681 gcgcctctcg tgatctccga atcccctggc agggcgagca cgctcttccc ctccaggtcg
     4741 aaggccgcga gagcggggtc gcagccgacg cgggccacga gggggacgcc gcccgtgtcc
     4801 aggaggtcgg ccgccgccgc gtcgggcagg cggttcgcga ccgctcccac cttctcgtac
     4861 atgacgagct cgtcggccac cttgcggatg gcgcggacga cctgggctcc cttgcggctg
     4921 gcgtcggtca cgaggaggag gtgggtcacc ttctccatca cgcggcggtt cacctgctcg
     4981 atgccggcct cgccgtcgac gaccacgtag tcgaagttct tcgacaggat cccgatgacc
     5041 tccttcaggt aggagttgat cctgcagtag cagccctcgg cctcgggccg gccgaccgcg
     5101 atgaaggcga agccctcggc ctccacgagg gcgtcgtaga tccggtaccg cgcctcgccc
     5161 aggagctcga cggcctcctt cgtgtcgccg tcctccacgg aggcgacgac ggccttgcgg
     5221 atgtcgtcga tggtggtctc gacctcgatc cccagcgcgg tcgagaggcc gaccgcggga
     5281 tcggcgtcta tcgcgatgat cctcttgccg gggaaggcct ccaccagcga tttcacgatg
     5341 gccgccgcga gcgaggtctt gccgactccg cccttgcccg cgacggcgat gattttggtg
     5401 acgccttccg cggttcctgt tccgctaacc atgaatttcc tcttggaatt atgcctattc
     5461 gcccgcggct tccctgtttt ctcgcgcagc atcgccggag gggcaaccca ccgtacagtc
     5521 gtcttttgaa acgtcaagca aaatttatag atatgttgag cgatgtgcga cgccgtagcg
     5581 gggccgcgga gaggaagttt gtgtttgcgt gacggatgcc gcgcggcgcg tcggtaaaag
     5641 cctgcggcgg ctctcggcgc gccgcgccgg cctctacggc cgagccgcgc cgcgcctcat
     5701 tcggagaact cgtcctccac catgccggcg agctcgtcta ccaggtcgaa gatgtactcc
     5761 gcgtcgccgt aggcgctctg cgcgtagacg agctccgcgg tctccgcgtc gatgacgcgg
     5821 caggacacgc gggcctggcc ggcgacgaag gagacggagc cgaggaggac gaagcgcgcg
     5881 ccggcgacgc ggcccgcgcg ggccgccgtc gcctcgtcga cgacgccgga gagcgagagc
     5941 gcctgctcgg cgagggcctt ctctagctgg cgccgctcga cgggccgcag gactcctccg
     6001 cggagcaagg cctccgcgac cgcctcgggc gcgtcggtcc tcaggctctc gtactcgggc
     6061 ccgcctccct tgagctcgaa gtcgaagacg gcgacgaggt cgccgcctgg gctgccctcg
     6121 gcttgcccct cgcccgcctc ctcggggcct cccgcgcagg aggaaaggac gaggagggcg
     6181 agagccgcgg acgcgaggaa tttccctttc atggacgacc ccctgtggac ccggccgcgc
     6241 cgggcgagga tcccgcgcgg gcccctcggc cgggcctgcc ttaagcctag cgccgctggg
     6301 ggctccggtc aaacgcggcc ctgcctctcg gggcctgggc ctgggcctcg ccgagggact
     6361 cgcgggcgac gcgcggcgcc gcgggcgacg cgcggcgaag gaggctcctt tcgagggcgg
     6421 ccctagcgca ggctgagcag cgctacgccg acgatggcga gcacgaggcc ggccgccctg
     6481 gcgagcgtga ccttctcctt gaggaagata cgtcccagga ggacggtcgg cgccgggtag
     6541 agggcagtca cgacggccac gagggtgagg aggcccgagc gggccgcgag gaggaagagg
     6601 atgttcgccc ccatgtcggc caggcccgcg accgccgtgg cggggccgtc gcctttctcc
     6661 agcctcgggc gcgagccgga ggcgaggagg gccgagaggg ccacgccgat ggaggccgtc
     6721 ctcgcggcga cgaggggcca gaggcccgag gccggggagg tcctggccgc ggcgacgaag
     6781 aagccgccga agccgaggcc cgcgaggacg ccgaaggcga gggccctcct cgactccctc
     6841 cgcgcggcgg ccttgtccgc gccctggccg tcgccgcttc ccgagccgag gaggatgacg
     6901 gcgggcaggc agagcgccgc tcccgcgagg gccgggcccg agggcctctc tccgagggcg
     6961 aggccgaagg ccatgggcac gagggcgccc acgagggccg aggatggaga gacgatcgcg
     7021 acgaggctgg tcgcgaggcc gcggtagagg aggaggagtc ccgccgagcc cgcgagtccg
     7081 gcggcgaggc cccagaggag gtcgcgcggc gcgggcaggg gcgtcccgga ggcggcgatg
     7141 aaggagagtg ccagggcgag gccgaggatc tgggagaggg cgacgacgga gaggacggag
     7201 gactttctcg cggcgtagcc gcccgagaag tccgcgatgc cgtaggcgag ggccgagagg
     7261 ccggcgagga ggagggccat gccccattct ggcacggccg cgccgtcccg ggctactcgg
     7321 tcacggcgac gcggctcccg cccgtgacgg cgaggagcct ctcgaaggag acggggacgc
     7381 cagtggagtc ggtgcccgcc gcgggataga cggtctcgta ggcggccagg ctttcgtcga
     7441 ggtggaccgc gacgccctcc agggcgaagg ggcagacgcc gccgggcggg tagcccgtga
     7501 cgcggagggt ctcctccgcg tcggccatgc tcgccttgga gccgaccagg gccttgagcc
     7561 gtcccgagtt cacgcgccgg tcgccggcgc agaccacgag gtggtagctc ccgtccttcg
     7621 cgcggaagag gagggacttc gcgatgcgcc cgacctccac gccgatgcgc gccgcggcga
     7681 ggggcgcggt gggcgtggag ccgggctcga actccagggc ctcgaggccc gctgccgcga
     7741 ggaccttggc cactttctcg ggaatcatgg gaggatggtg gcccctctgc cgcggcccgt
     7801 caagacgcgc cggccgcggg cggcgcgggc cgtggacggc ggcgccgccg cgccgactgt
     7861 cacgcgcgcc gcggcgccga tcgccgcgcg gcccgcgggc tcggcccagg cgcaggagcg
     7921 ccgaggcggc gcggccctca ggccgccggc cgagccggga cgctgaagcg gctaggccct
     7981 cggccggcaa gcggcgtggc ggctaggccc cgaggccccg cttgcagagg aagtcgcaaa
     8041 agaaacagat gtcggccgtc ttcccgggga aggcgaagcc ctccgcctcg agccgcttcc
     8101 tcacctcgcc caggcccgtc tcgtagcagg cccgcacgcg ggggctcgcc gcggcccgcg
     8161 cgaggacggc gtcgacgccg tctccgggga ttcgcccgac gacgagctcc tcgttctcgt
     8221 tcgcgaagcc gcagcagacg gcgaccttgc cgttcggatg gacgtagagg acgttgcccg
     8281 gccccgcgca gaagtcgtcc gtgaaccact ccttcgccga ccaggggccg gaggcgggcg
     8341 tgcctccggc gccggctgcc tggccgcccg ccgccgctcc gtccgccgcg ccgggctcgc
     8401 ccgcggcgga gtaggggatg cggacgatgt cgacgcggag ctcctcgccc tcggcggccg
     8461 cccgggcgcc gcggcggccc gcgcccttgg ccgggacgat ggcctccggc tcgccgcccg
     8521 agaggacgag ctcgccgccc agggcggcgg cgacggcgcg gaagatgtcg aggagggggc
     8581 cgtcgtccgg cgagacgacg gaggcgatct ccacgcagtc gcggcggccg aaggcgcggt
     8641 gcgcggcctc gatgaagggg aggaggcggc cggcggtgga gccgtgccag cggtcggcgg
     8701 agaggccgat ggtcccgtcg aagccggcct ccgcgacgct ccccagggtc tcctcgagct
     8761 cggcctcgtc ccgccaccag gagccgttcg tcatgaggcg gtcgaagagg aggcccaggg
     8821 agacggcctc cgcgcagagc tcgcggagga agtcgggcct gaggaagggc tcgccgccgg
     8881 agaagcccag gcgcccgacg ccgcgctccg cggcctcgcg gaggaaggcg accgcgtcgg
     8941 acgccgcgag ctcctcgggc acgcgggcga cgcggcaatg cgcgcaggcc aggttgcagc
     9001 gcgccgtcgg cgcgaagatg atctcctccg gcttgaacat gggcacccct cgccgggccg
     9061 ccgctcgcgg ccgcccgacg ccagcaggat agcgcggatt tgcgggaagt tgaaggagcg
     9121 cctggggaag gccgtctgcg gcgcctggac gactgggagc ggtttccgag gaggggccgc
     9181 gccaggcgcc cgccttcgag ccaggcgccg ggctcggctt cgtccgcctg cgcggcgcgg
     9241 ttccgtacgc ctgcgcggcg cggcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnctgc gcggcgcggc ccggccatgg gcctcaaggc ccgggaggcg gaaccccgtt
     9421 gcctgccgcg ggcaggagga ggagaaaggc cgcgcccccg agctccgact cgccgagctc
     9481 gatccggccc cccatggcct tggcgagtcc gctcgcgatg gagaggccca ggcctcgtcc
     9541 gcccgtgccc gacgagcggg aggcgtccag gcggtagaag cgctcgaaga cgcgctcccg
     9601 ctcggcgggc gggacgccgg ggcccgagtc ctccaccgcg aattcgacga agggggccgc
     9661 cgtacgccag ccgccgtcgc ggtcccgccg cgcgggcggg tcgggcgcct ggcgctccct
     9721 cgcgaggagc ctgaccctgc cgccctcggg ggcgtgcctg accgcgttcg cgaggaagtt
     9781 cgagaggatc tcgcgcaggc ggaaggcgtc ggcgagcacg cgggccgcgc cctcccccga
     9841 gacgcagagg gaaaggccct tggcgcgggc cggcgcttcg aagagggcgg ccgcgccccg
     9901 cgcctcctcc agggcgtcca gctcctcgat ccggagctcg agctcccccg actcggcgct
     9961 ctcgagctcg cgcagggact cgatgaggcg ggccaggcgg agggcctcct cgtggacgga
    10021 gcgcaaggtc tcgtcgtcgc gggggaagac cccgtcgagc atcgcctcca cggccccctg
    10081 gatgagggcg gcgggggtcc tgagctcgtg ggcggcgtcg gcgatgatgc gccgcttggc
    10141 ctcctcgagc cgcgcgagct cggcggccat ggcgttgaag gagcccgaga gctcggcgac
    10201 ctcgtccccg ccgcgggcgt cgacgcgagc ggagaggtcc cccgcctgga ccctcctggc
    10261 cgcccgggcc agggcggcca ggggccgcgt catgcgcgcc gacaggccga gccccaggag
    10321 gagggccagg gccgcggaga ggagggtcgc cgagagcacg gcggagaaga cccgcctgag
    10381 gaagcgctcg cccggggcgg tcagggagga ctcgatcatc gagccgacaa ggacggtgcc
    10441 gacgcggcgg ccgcccgaga ggacggggag gccgtgggcg aggtgggcgg ggggatggac
    10501 gctcccgagg agcctccccg cggtgtcgac gacgacgacg ccgtcggggt ccgccagggc
    10561 gacgcggtcc gagaggaggg cgtaggcgga ctccgccgcg gccgctgctt cggcgcccgc
    10621 ggagagtccc tgtcccgcgg tcggcgcttt cgccgcgccg gcgtaggcgg ccgcgtcggc
    10681 caacgcggcc gtagaggcct cgggggccgc ccggcgcgcg tggacgctct cctcgatcga
    10741 ggccaggagg cgggcgggca tcccggcgac gaaggcctgg gcgccctccc agttcccctt
    10801 ctcatcgtag aagtcggcga gaagggagcc gtagacccgc gccttctcgg cgtccccgga
    10861 gaagacgaag gagcggaagg cgctctccgc cgccggcccg accatgagga gggcggcggc
    10921 cgagagggcc gcgatcacgg acaggaagga gagggcgagc ctggccctaa tccgcatcgc
    10981 gcggctccat gaagcggtag ccgacgccgc ggacggtctc cacgaagcgc ggggccgcgc
    11041 tgtcgtcgcc cagggccttc ctgatgttct tgacgtgggc gtccaccgtc cgctcgtagc
    11101 cctcgaaggc ctcgccggag gcgctctcca ggatggaaag gcggctgcgg actcgtcctg
    11161 gctcccgcgc caggaaggcg aggatgtcga actggaccgc ggtcagggcc ttgggctcgc
    11221 cgtcgacgcg cacggatcgc cggccgaagt ccatctccag ggagccgcgg cgcaggacct
    11281 cctcgggggc cgcgccctcc ctccggccgc gcctgaggac ggcccgtacg cgggcgacga
    11341 gctcgcgggg gctaaagggc ttggtcacgt agtcgtccgc cccgagctcg aggccgagga
    11401 cgcggtccgc ctcctcggcc ctggcggtga ggaagatgac gggcaggtcc gaggaccgcc
    11461 tgatttcccg ggcgagctcc aggccgtcga gccccggcat cgagatgtcg aggacggcga
    11521 ggtcgggctt ccgctccctg aaggccgcca gggcggcgag gccgtcgaag gccagggccg
    11581 cctcgaagcc cgaggcctcg aggtagctgc cgaccatcct ggcgatcttc tcctcgtcgt
    11641 ccgcgacgag gacgagtccg cgcgccacgc cggattcccc gtcctagcgg agctcgaagg
    11701 gccgtaggag cttgtcgacg aggagcttct tgcccttggc gtccatcgtc tccaccttgg
    11761 cgaagaccca gccttcgacc ttggcggggt cgaggcccgc ggcgacgagg gggccgggct
    11821 cgagcacgaa caccaggtcc ttgtcgttct tcgagaggtc cttggcccac tccaccatgt
    11881 tgccgccgcc gagcatgata ccgtagtggt ccagggcggc gtggtaggag acgcgggcgc
    11941 gctcggcctt gaggagctcg ccgaaggcgg ccgtcatgga gtccttggct gcgggggcga
    12001 aggccgcctt tccgagctcg aagtggagca tgaagcgtcc atcctcaagt tcgtacttga
    12061 tgccttcggc ggcggggatc ttcgcgacgt ccaggccggc cgcgaggaag ggggccgcgt
    12121 ccaggctgag ctcggcgtcg ggcctgtcca tgtcggccat gccgcccgag cccgcgttgc
    12181 gggcgaaatc gctcgagagg aggaagaggt cgcccgcggg ggaggcgagg gcccaggccc
    12241 cgtcctcggc ctcgtaggag atcctgtccc tcgaggcctc ggcgaggctc gcgaaggagg
    12301 aggccgcgaa cttgccgacg gtgtcggtgg ctgcgcagcc ggcgagggcc gcggcggcgg
    12361 ccagggccgt aaggacggcc gcgctgcgaa tgccgtgaat cttcatgtcg tttccttcgc
    12421 cgcgcctcgc gcggcttgtc ttgtcccgcc cggactccag gccggctcgc cggcctgagt
    12481 cccgtcgttc ggggtcaatc cctgccggcc tagcggccgc ccaggacctc gctgaccgag
    12541 tagccggcct cggcgacggc ggccttgagg gccgcgtcgg agagggcctc gccctcgacc
    12601 atcgcgcttt tcttgacgag gtcgacgtcc accttggcga cgccgggcac ctcggagagg
    12661 gcggtcttca cgtgcatgac gcagtggccg cagctcatcc cctcgatcgt cagaagcttt
    12721 ctcatactga tcctccttta cacgcgatac ggcttgaagc gcttgagcct cagcgcgttg
    12781 gtcaagacgg agacggagct catcgacatg gcggccgcgg cgaagatcgg gttgaggagc
    12841 gggcctccga aggcgtagag gaggccggcc gcgaccggga tgccgaggac gttgtagccg
    12901 aaggcccaga agaggttctg cttgatgttc cggatgacgc tccgggagag cttgatggcc
    12961 gtgggcacgt ccatgaggtc gctccgcatg aggacgaagt cggcgctctc catcgccacg
    13021 tccgcgcccg agccgaccgc gatgcccacg tccgcctgcg cgagggcggg cgcgtcgttg
    13081 acgccgtcgc ccaccatcgc caccctgcgg ccggcggcct ggagcttctt gacctccgcg
    13141 gccttgtcct cgggaaggac ctcggcgagg acgcggtcga tgccgacccg ccgggcgatg
    13201 gccgcggccg tccgtgcgga gtcgcccgtg atcatggcga cctcgacgcc catggcgtgc
    13261 agggcggcga tggccggagc gctcgactcc ttcacgacgt cggccgccgc gacgaggccg
    13321 gcgtagcgcc cgtccaccgc gacgaacatc ggcgtcttgc cgtcctccgc gagggaggcg
    13381 gccttggcct cgccgtcgcc tgagcctatg ccccgctcgg cgaggaggcg cgcgctgccc
    13441 gcgagcacct cgcggccgcc gaccttcgcc tcgatgccgc ggccggggag ggcggagaag
    13501 ctctccgcgg gggggagggc gaggccccgc gcctcggcgg ccctgaccag ggcctcgccc
    13561 aggggatgct cggaggcctt ctccgcggcg gcggccaggg agaggagctc gtccccgccg
    13621 aagccggggg ccgggaccag gtcggtgagc tcggggctgc ccttcgtgat cgtgccggtc
    13681 ttgtcgaaga ctacggtgtc gacgcggtgc gcggtctcca gggcgggccc cgacttgatg
    13741 agtacgccga gctcggcgcc gcggccggtg cccaccatga tggccgtcgg cgtcgcgagg
    13801 ccgagggcgc aggggcaggc gatcgtgagc accgcgacga agaccgtgag ggcgaagacg
    13861 ggcgtgtggc cgaggaggag ccaggcgacg gcggagagga gggcgatccc gaggacgatg
    13921 gggacgaacc agccggagac gacgtcggcc aggcgggcta tgggcgcctt ggagccctgg
    13981 gcctcctcca cgaggcggac gatgcgcgac agcacggtgt cggcgccgac ctcggtggcg
    14041 cggaagacga gggagccgtt cttgttgagg ctggcgccga tgacgcggtc gccggcctcc
    14101 ttctccaccg ggatgctctc gcccgtgagc atggactcgt ccacggcgcc gcggccggag
    14161 acgacgaggc cgtccacggg gatcttctct ccgggcctga cgcgcacgag gtcgcccgcc
    14221 tcgacctcgt cgacgggcac ctcgagctcg gtctcgccgt ggacgaccgt ggcggtcttg
    14281 ggcctgaggc ccatgagctt cttgatggac tccgaggtcc tgcccttcgc gacggcctcc
    14341 agggactttc cgaggaggat gagggtgatg atgacgcccg cggtctcgaa gtagaggctc
    14401 tccacggcgc ggaaatcgcc ggcggcgatc ctggcggccg agtagaggga gtagagcacg
    14461 gcggcgctcg tcccgagggc gatgagcgaa tccatgttgg gggcgccccg gaagagcgcc
    14521 ttgaagccga ccgagtagaa gcggcggccg gcggcgacga cggggacgac gaggaacagc
    14581 tggacgaggg cgaggcggag cgggtaggcc atcatggaga ggcccgcggg gaggggccag
    14641 ccgaacatcg aacccatcgc gatgtagagg aggggggccg agaagaccgc ggagaccgtg
    14701 aacctcctcc acaggccgcg gacctcgcgc tccttctccg ccttgtgctc gtcgaccttc
    14761 tccccgccgt ccgcgagggc caggggctcg tagccggcct tgacgaccgc ggccctgatc
    14821 tcggagaggc ggaggcgggc gggatcgtag gagacgcggg ccttctccgt cgcgtagttg
    14881 acggaggcgg cggagacgcc ggggagcttc ccgatggcgc gctcgaccgc cgcggcgcag
    14941 gaggcgcagc tcatgccgcc gatgggcagg gagatctccc gggcctcgac gtcgtcgacc
    15001 gcctcgtagc cggccttcgc gaccgcggcc ttcaccgcgt ccagggagag ggccgcatcg
    15061 tcccagctca ccgagaggcg ctcggtggcg aagttgaccg aggcctcggc gacgcccgga
    15121 agcttgcgca ccgcccgctc cgaagccgtc gcgcaggc
//
LOCUS       DAOOSJ010000027        15055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2685_length_15055_cov_5.400333, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000027 DAOOSJ010000000
VERSION     DAOOSJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2685_length_15055_cov_5.400333"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLG14_02600"
     CDS             <1..993
                     /locus_tag="PLG14_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX48051.1"
                     /translation="WIKPENWVGNGAYVVTEWKPQQHLFVTKNAKYWDAKNVKIKSIK
                     FLPIEDNKTAYNMFKNGEMDWNTGVALDLIDEIKLRKDYQVSPQLSTYYYIFNVTKKP
                     VDDVRVRKALMMAVDSKTLVEKVTKGGQLPTANMVPAMEGYTPAKGNPFNVEEAKKLL
                     AEAGYPNGKGFPKFSILYNTHDGHKKIAEFVQEQWKQNLGIEVSLKNEEWKTFLDTRA
                     NSHDFVVSRAGWTGDYLDPNTFLDLFIIGSGNNDGLYGSQEYTDLVKKAMTMPAGPER
                     LAVLSQAEELLVGKDQAIAPFYFYVAQNLVDTEKWGGWYANPLDIHNWKFISKK"
     gene            1319..2902
                     /locus_tag="PLG14_02605"
     CDS             1319..2902
                     /locus_tag="PLG14_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX48052.1"
                     /translation="MKRIFAVLFALIALSSLLFVSCGQKELTADKAEFIINNGAEPQS
                     LDPSHIQGVPEHRIYMALFDGLVLNDPKTCYAVPGVAESWTTSPDGLTITFKLRKSTW
                     SDGTPVTAKDFVDGWLRTLDPATGAEYAYMIGMVVKGANEFNGGTGPKEGVAVKAIDD
                     RTLEVGLTGPAPYAVDMMAHYAFSPLPMHAIAKFGDDWIKPENWVSNGPYLVKEWKPQ
                     QHLFVVKNAKYWDAKNVKLKSIKFLPIEDNKTAYNMYKNGEMDWNTGVALDLLDEIKL
                     RKDYMVAPQVATYYYIFNTTRKPTDNVLVRKALAMAIDTTTLVEKVTKGGQIATSAMV
                     PAMEGYTPAKGNPYNVEEAKKLLAEAGYPNGKGFPKLTILYNTNDGHKKIAEFVQEQW
                     KNNLGIEVSLKNEEWKTFLDTRSNSHDFVVARAGWVGDYLDPNTFLDMFIVGSGNNDG
                     LYNNAKYDELVRKAMTMPSGKERLAVLGEAEKLLLDQDQAVAPFYHYVEQHLIDTNKW
                     GGWYNNPLGIHNWKFIYKK"
     gene            3140..4069
                     /locus_tag="PLG14_02610"
     CDS             3140..4069
                     /locus_tag="PLG14_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48053.1"
                     /translation="MTKFIIRRLLSLIPTMFIIVTLSFFLIRFAPGGPFSSEKKLPEQ
                     VLQNILKKYHMDQPLGKQYLSYMGDVLRGDLGPSYRYKDQTVTELIGQTMPNSIFLGT
                     LALGMALLAGISAGVVSALRQNKWQDYTAMSLAVIGISVPLFVIGPILQLVFAMKLHW
                     LPTSGWITGRAGALTLIMPALTLSFPYFAYIARLSRGSVLEVLRSDYIRTARAKGLKE
                     SVVVWKHVLKGALLPVVSYIGPAFAGIITGSVVVETVFVIPGVGKIFVQSALNRDYTL
                     IMGDIIVYSLFLVVMNFVVDIVYGFLDPRIAYK"
     gene            4079..5149
                     /locus_tag="PLG14_02615"
     CDS             4079..5149
                     /locus_tag="PLG14_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48054.1"
                     /translation="MSPKKNQAVNEFNQQVKPVSLWADAWKRLRKNKMALFGLWLVVA
                     YVAVSLAAPILPLYDFTSQELTHVNLPPSLKPAGEVALGRLQKRMADLKEAGAFREDL
                     KTEAAFVQAEYDKLTVEIATNPVHKRVYILGTDYLGRDMLARTIYGGRISIMIGLVGT
                     ITAVFIGIIVGAIAGYVGGRTDNLLMRFVDILYGLPYMLVVIILMSMVPEGTNSIIVL
                     FVAIAALSWLTIARVVRGQIISLKNAEFVEAARSMGASSGRIIFRHLLPNTLGVIVIF
                     ATLSLPSFIMNESFLSFLGLGVSAPSASWGTLVSEGVQGMELYPWRLLVPAVAMTAFL
                     FAMNFLGDGLRDALDPQSKNRT"
     gene            5174..6157
                     /locus_tag="PLG14_02620"
     CDS             5174..6157
                     /locus_tag="PLG14_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48055.1"
                     /translation="MTEDVILQVKDLKTYFHVDEGIVKAVDGVSFNLRKGETLGIVGE
                     SGSGKSVTNLSVINMIPNPPGKIVGGEVLFMGKDLIKMPVSELRSIRGNKISMIFQDP
                     MTSLNPFLRISTQMVETIVLHQGLDKKAAKEKAIEMLKLAGIPAPEKRIDQYPHQFSG
                     GMRQRVMIAMGLSCNPEILIADEPTSALDVTIQAQILDLMKDLTARLGTAVILITHSL
                     GVVAGMCDTLCVMYAGRVVERGLTSEIFAEPKHPYTQGLIKSVPRLDQASGDRLNSIR
                     GQPPNVIDLPDCCPFYPRCDKAMDICKRKYPAATEYGNGRAVSCWLYTEAK"
     gene            6160..7167
                     /locus_tag="PLG14_02625"
     CDS             6160..7167
                     /locus_tag="PLG14_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48056.1"
                     /translation="MTENEVLLDVKDLKMHFPIGSKSMIGPHKNFIYAVDGVDFKIKK
                     GETLGLVGESGCGKSTTARAVAQLYKPTAGEVILKGKDLTKLKANDLIAARKNMQMVF
                     QDPYASLNPRMTSGDIIAEPMRIYQMKGILQMTKKEMDERVEQLMERVGLSRFFRNRY
                     PHEFSGGQRQRIGIARALALRPELVLCDEPVSALDVSIQAQILNLFKDLQDEFGLTYL
                     FIAHDLAVIQYISTRVAVMYLGTVAEISGSAELYKKPLHPYTQALLSAAPIPDPKIEK
                     ERKRIILTGDVPSPDKQRPGCPFYDRCWKHMDKCKDHKPRLREAAPGHEVACFLYHDP
                     D"
     gene            complement(7365..7943)
                     /locus_tag="PLG14_02630"
     CDS             complement(7365..7943)
                     /locus_tag="PLG14_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48057.1"
                     /translation="MDIPLVLKSGREGRLVYSIPLWYRLSMIVMAGILVAAAFASGGA
                     PGPLGWIALALAVLAALYEERWILDPGAGLLRHRFGLLVLARSVALPFDRIEAFRLRS
                     FVRGSVPGSGEEAAIKRDALASADDRGDASAEAKSGRRRHKKTYVSLVCDDLEGGGLV
                     LNILPASRVERLREVGRRLAAAAGKPFSEGDD"
     gene            8148..8654
                     /locus_tag="PLG14_02635"
     CDS             8148..8654
                     /locus_tag="PLG14_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX48058.1"
                     /translation="MANSRVQFETSLGSLVIELFEDSCPVTTANFLDYVRSGFYDGTI
                     FHRVIPGFVVQGGGMLPGLKEKDKGKAPIKNEAAKGPANSRGTLSMARTSDPDSATSQ
                     FFINLVDNKSLDYSGPGPRGAGYAVFGKVVEGMEAVDSMAKVATGSRFPHQDVPKEDI
                     VLVKATVL"
     gene            complement(8667..9728)
                     /locus_tag="PLG14_02640"
     CDS             complement(8667..9728)
                     /locus_tag="PLG14_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOX48059.1"
                     /translation="MDSRIIEALAGAEGSLALATVLSVQGSAPRHPGTKLLARGPADF
                     VGTVGGGKGELLALEEAGRRLAAGGASLIEVEMLGSVATGAALICGGLCRILVERPEP
                     SQAPAYAEAARLLASGRRALLVKRLSPRGEGYAVELSVLSEDGKAVFGSLSGIDPRAA
                     EAALGKGAARFSEKDLVFYDPLFPREKLLVLGGGHVGRALAAAALPLGFEVSVADDRE
                     GFPLPGQLPPEVRPLPGPFAKTIAAFSFDPATYVVILTHGHLSDLDCLRAVLPREYRY
                     AGMIGSARKTRVLLDAAKAEGFDPARVDAVCAPLGLDVDAETPAEIAVAALCEMVAYR
                     RNSGILPRLAAEREARRSP"
     gene            complement(9750..10091)
                     /locus_tag="PLG14_02645"
     CDS             complement(9750..10091)
                     /locus_tag="PLG14_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOX48060.1"
                     /translation="MAVDETLVSENIAEMSRTLAKVDDPTLVQGFLFSLFTPSEADEI
                     AKRWALVKDLAAGIPQREIAKELGLSLCKITRGSRELKKEASPFKRMLELAGYEVPEV
                     RPRGKPKAKPE"
     gene            10214..10597
                     /locus_tag="PLG14_02650"
     CDS             10214..10597
                     /locus_tag="PLG14_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOX48061.1"
                     /translation="MLTFLRRTRLATIVATLVVLAALLWPGEDLPKVGLAGLDKAAHL
                     LLFGAWALALRFDWPGLRARPWLLVLAVALFAPLTEGLQLLAPQRSFDLLDAAADLCG
                     AVLAAAFGARPVALAERLLSPRKAR"
     gene            complement(10594..12756)
                     /locus_tag="PLG14_02655"
     CDS             complement(10594..12756)
                     /locus_tag="PLG14_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOX48062.1"
                     /translation="MAVVVVEKLSPSRAPGGYAEDCAALTRDYGTAAWESRPEVAEIR
                     RAVFAALDRLEAETGLGGKLKGRPVLVKPNLVTVYHRMGLARRTYPESTDPRVLDAAL
                     LWLGERASSLTIVESSGRGAPTRGSFRVAGVDRLARRRGAKLVALEETPVDRYLLPKA
                     RVQREILVPRPFSAVVRGEAAYVSLPKLKTNLYTGVTLGFKNAMGVIPYNLRQRNHHY
                     AIDRKLVEMLYLFKPDLVLVDGVVGGEGECPAPVDPVDSRMVIAGDHPVETDRVATRL
                     MGHDPSRIELMRVADELGFGDPEGARVLARGFGLPAGAAPGDAGPGGAAPNACAPDGD
                     GLGGAVPVAFRPADWSLHSERVRKGFPSVLVLIGIDKGLAAAGGGQCGGACGAGGASD
                     AEGGALGEGGLPGGGAAAPAFVRGLEASCRGGCVATTRFAFSMLEHEGIRPRRRAVLV
                     LGAGVPTAQGRRWYDAEGKAYDEAAIAALPGRKAVIGSCGRAAARLAERFVDGCMPLP
                     NAPHMVLHELVGRPCRVMSLRNENLFPMLAATLGARRARQRLIEEGCRLDVPLGLEDG
                     LVPGRALAAGEEGRDWLPWPLPPLGAEEARRLLAFEDDAALAAFRGVLVFRVKERLLW
                     RAKALLTGTATLAPLALAAAGAAGAGLWLAPAAWLGMFLAVEALHACELPASLRARRL
                     RAMRRGRTWGRADAAAAVAATLAVGYPAWLPHRHGVFD"
     gene            complement(12776..14926)
                     /locus_tag="PLG14_02660"
     CDS             complement(12776..14926)
                     /locus_tag="PLG14_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOX48063.1"
                     /translation="MPGSATGRVLSVDLTTGKIEAVEVPEEVYRDFLGGMGLGAWWLY
                     TRMPAGADPLGPEAVLGFVPGLLTGSGSLYTGRWMAVCKSPLTGGWGDANCGGFLAPA
                     LKRSGYDGVFVSGVSEKPVYLYLGNGKAELRDASALWGLDAVATEERLQAELRKKRPG
                     RRPEVAAIGRAGESLSLIAGIVSDGGRIAARSGVGAVMGSKRLKALVAAGTRPIPCAD
                     RDGVRARSAAFARRLPRGLPLPPASVLGQGGALLRKLPLALRFDGFLFGEVLSKWGTA
                     GMNRLSVEWGDSPIRNWSGSEKDYPAAKSRRIDADLVLAAQKRAYHCYSCPTGCGGIC
                     EHEGKETHKPEYETILALSGLLLCDDLPAVFRMNERLNRAGMDSISAGACLAMALECA
                     EAGLVPRELPGGLELRWGNAQAVELLLGQMLDREGLGAIFADGVKKAAERLGPAAAGL
                     AVHAGGQELAMHDPRNDPGFALHAAVDPSPGKHTVGCHLYYGMWRLWKRLPGAPRPPL
                     LSRKSEEYEASPLAARKAVLSSALVSLFNAAGLCLFGSLYGVDRLGLFESLDAATGLA
                     RSPEDYVEAGLRIQTLRQLFSLREGIDPQSLMPSKRALGLPPKAEGANAGRSVPLPRL
                     VEDYWKEIGWPSGRPSPETLERLGLAGLAAGAGAPRRTVAGEGAAAGTAALGQPAAGG
                     GSPAADRAAPAGAGADSAAPAADRAPGAGPAAKP"
BASE COUNT         2514 a         5323 c         5038 g         2180 t
ORIGIN      
        1 tggatcaagc cggagaactg ggtcggcaac ggcgcctacg tcgtgaccga gtggaagccc
       61 cagcagcacc tcttcgtcac gaagaacgcg aagtactggg acgccaagaa cgtcaagatc
      121 aagtccatca agttcctccc catcgaggac aacaagaccg cctacaacat gttcaagaac
      181 ggcgagatgg actggaacac cggcgtggcg ctcgacctca tcgacgagat caagctccgc
      241 aaggactacc aggtctcccc ccagctctcc acctactact acatcttcaa cgtgaccaag
      301 aagccggtcg acgacgtccg cgtccgcaag gccctcatga tggcggtcga ctccaagacc
      361 ctcgtcgaga aggtcacgaa gggcggccag ctccccaccg cgaacatggt tcccgcgatg
      421 gagggctaca cccccgcgaa gggcaacccc ttcaacgtcg aggaggccaa gaagctcctc
      481 gccgaggccg gctaccccaa cggcaagggc ttcccgaagt tctccatcct ctacaacacg
      541 catgacggcc acaagaagat cgccgagttc gtgcaggagc agtggaagca gaacctcggc
      601 atcgaggtca gcctcaagaa cgaggagtgg aagaccttcc tcgacacccg cgcgaacagc
      661 cacgacttcg tggtctcccg cgccggctgg accggggact acctggaccc gaacaccttc
      721 ctcgacctct tcatcatcgg ctcgggcaac aacgacggcc tctacggcag ccaggagtac
      781 accgacctcg tgaagaaggc catgacgatg cccgccgggc ctgagcgtct cgcggtcctc
      841 tcccaggccg aggagctcct ggtcgggaag gaccaggcga tcgcgccctt ctacttctac
      901 gtcgcccaga acctcgtgga cacggagaag tggggcggct ggtacgccaa ccccctggac
      961 atccacaact ggaagttcat ctccaagaag taattcgatc ctagccggat tccgggcccg
     1021 gccttgcggc cgcccgggat ccgcggcgcg gagggacggc ttgccgcccc tcccgcccgc
     1081 ccggaccagg gcgatgagca cgggtccggg aaactgtcaa gaaatatcac gtatttgggg
     1141 tcgttgatcg gtttagtcag gcaatttggc tatcttgttt ctgcgcgaag atatgagcag
     1201 gccgtctcat aggtctgcct aggggccttt cccagggctc gcgaaaaaag cggttgcgct
     1261 cgggaaaaaa gcggggtata acgactagca tctcgcatcg gcatcatagg aggtatctat
     1321 gaagaggata ttcgcagtcc ttttcgcctt gatcgccctg tcgtccctgc tcttcgtctc
     1381 ctgcggacag aaagagctca ccgccgacaa ggctgaattc atcatcaaca acggagccga
     1441 gccccagtcg ctggatccct cccacatcca gggcgtcccc gagcaccgca tctacatggc
     1501 gctcttcgac ggcctcgtcc tcaacgaccc caagacctgc tacgccgtcc ccggcgtcgc
     1561 cgagtcctgg accacgagcc ccgacgggct cacgatcacc ttcaagctcc gcaagtccac
     1621 ctggtccgac ggcacccccg tcacggccaa ggacttcgtc gacggctggc tccgcaccct
     1681 cgaccccgcc acgggcgccg agtacgcgta catgatcggc atggtcgtca agggcgcgaa
     1741 cgagttcaac ggcggcaccg gtcccaagga aggcgtcgcg gtcaaggcga tcgacgaccg
     1801 caccctcgag gtcggcctca ccggccccgc cccctacgcc gtcgacatga tggcccacta
     1861 cgccttctcc ccgctcccga tgcacgccat cgccaagttc ggcgacgact ggatcaagcc
     1921 cgagaactgg gtctccaacg gcccctacct cgtcaaggag tggaagcccc agcagcacct
     1981 cttcgtcgtg aagaacgcga agtactggga cgcgaagaac gtcaagctca agtccatcaa
     2041 gttcctcccc atcgaggaca acaagaccgc ctacaacatg tacaagaacg gcgagatgga
     2101 ctggaacacc ggcgtcgcgc tcgacctcct ggacgagatc aagctccgca aggactacat
     2161 ggtcgccccc caggtcgcca cctactacta catcttcaac acgacccgca agcccacgga
     2221 caacgtcctc gtccgcaagg ccctcgccat ggcgatcgac accaccaccc tcgtggagaa
     2281 ggtcaccaag ggcggccaga tcgccacgag cgccatggtc cccgcgatgg agggctacac
     2341 ccccgcgaag ggcaacccct acaacgtcga ggaggccaag aagctcctcg ccgaggccgg
     2401 ctacccgaac ggcaagggct tcccgaagct cacgatcctc tacaacacga acgacggcca
     2461 caagaagatc gccgagttcg tgcaggagca gtggaagaac aacctcggca tcgaggtcag
     2521 cctcaagaac gaggagtgga agaccttcct cgacacccgc tcgaacagcc acgacttcgt
     2581 ggtcgcccgc gcgggctggg tcggcgacta cctcgacccg aacaccttcc tcgacatgtt
     2641 catcgtcggc tccggcaaca acgacggcct ctacaacaac gccaagtacg acgagctcgt
     2701 gcgcaaggcc atgaccatgc cctccggcaa ggagcgcctc gccgtcctcg gcgaggccga
     2761 gaagctcctt ctcgaccagg accaggccgt ggctcccttc taccactacg tcgagcagca
     2821 cctgatcgac acgaacaagt ggggcggctg gtacaacaac ccgctcggca tccacaactg
     2881 gaagttcatc tacaagaagt gatcccgcgc gcggcgcccc ccggggcgcc gcggcatccg
     2941 ggctcccgga agggagcccg acgacgaaac gccacggagg acactccggt gtctcccgtg
     3001 gcttttttga tggaatagga atgcttgagg cgcccgaagc gcgccgatcc ggcgggagca
     3061 ccgcgggatc gtcgtactcc gcgctccttg aaacgcacag cgagcagcgg caatccctct
     3121 ctcggaggaa aaggaagcga tgaccaagtt catcatccga cgattgctga gcctcatacc
     3181 aacgatgttc atcatcgtga cgctcagctt tttcttgatc cgcttcgccc cgggaggccc
     3241 cttctccagc gagaagaagc ttccggagca ggtgcttcag aatatcctta aaaagtacca
     3301 catggaccag cccctcggga aacagtacct gagctacatg ggcgacgtcc tgcgcggcga
     3361 cctcgggccc tcgtaccgct acaaggacca gaccgtcacc gagctcatcg gacagaccat
     3421 gccgaactcg atcttcctcg gcaccctggc cctgggcatg gcgctgcttg ccggcatctc
     3481 cgcgggagtc gtatccgcgc tgaggcagaa caagtggcag gactacaccg cgatgtcgct
     3541 ggctgtcatc ggcatctcgg tcccgctctt cgtgatcggg cccatcctgc agctggtctt
     3601 cgcgatgaag ctgcactggc ttccgacctc gggctggatc acgggccgcg ccggcgcgct
     3661 caccctgatc atgcccgccc tcaccctctc cttcccctat ttcgcgtaca tcgcccgcct
     3721 gtcccgcggc tccgtcctgg aggtgctccg ctcggactac atccggaccg cccgcgcgaa
     3781 gggcctcaag gagtcggtgg tcgtgtggaa gcacgtcctc aagggggccc tcctccccgt
     3841 cgtctcctac atcgggcccg ccttcgccgg catcatcacc ggctccgtgg tcgtagagac
     3901 cgtcttcgtc atccccggcg tcggcaagat cttcgtccag tcggcactga accgcgacta
     3961 caccctcatc atgggagaca tcatcgtcta ctccctcttc ctcgtggtga tgaacttcgt
     4021 ggtcgacatc gtgtacggat tcctggatcc gcgcatcgcc tacaaatagg agagctgcat
     4081 gtcccccaag aagaaccagg cggtcaacga gttcaaccag caggtgaagc cagtctccct
     4141 ctgggccgac gcgtggaagc gcctccggaa gaacaagatg gccctgttcg ggctctggct
     4201 ggtcgtcgcc tacgtcgcgg tctccctcgc cgcgccgatc ctccccctct acgacttcac
     4261 gtcgcaggag ctcacccacg tcaacctccc gccctcgctc aagccggccg gcgaggtcgc
     4321 cctggggcgg ctccagaagc gcatggccga cctcaaggag gccggcgcct tccgcgagga
     4381 cctcaagacc gaggccgcct tcgtccaggc cgagtacgac aagctcaccg tcgagatcgc
     4441 cacgaacccg gtccacaagc gggtctacat cctgggcacc gactacctcg gccgcgacat
     4501 gctcgcgcgc acgatctacg gcggccgcat ctcgatcatg atcggcctcg tgggcacgat
     4561 caccgcggtc ttcatcggca tcatcgtcgg cgcgatcgcg ggctacgtgg gcggcaggac
     4621 cgacaacctc ctcatgaggt tcgtcgacat cctctacggc ctgccctaca tgctcgtcgt
     4681 catcatcctc atgtcgatgg tgcccgaggg gacgaactcc atcatcgtcc tcttcgtcgc
     4741 catcgccgcc ctctcctggc tgacgatcgc ccgcgtcgtg cgcggccaga tcatcagcct
     4801 caagaacgcg gagttcgtcg aggccgcccg ctccatgggc gcgagctcgg ggcggatcat
     4861 cttccgccac ctcctcccga acacgctggg cgtcatcgtc atcttcgcca ccctctccct
     4921 gccgagcttc atcatgaacg agtccttcct gtcgttcctg ggcctcggcg tctcggcgcc
     4981 ctccgcctcc tggggtaccc tcgtctccga gggcgtccag ggcatggagc tctatccctg
     5041 gcgcctcctc gtccccgccg tcgcgatgac cgcattcctc ttcgcgatga acttcctcgg
     5101 cgacggcctg cgcgacgcgc tcgacccgca gagcaagaac cggacctagg gtccgcctaa
     5161 ggagccaatc gaaatgaccg aagacgtcat tctccaggtc aaggacctaa agacctattt
     5221 ccacgtcgac gagggcatag tcaaggccgt cgacggcgtg agcttcaacc tccgcaaggg
     5281 cgagacgctc ggcatcgtcg gcgagtcggg gtcgggcaag tccgtcacga acctctccgt
     5341 catcaacatg ataccgaacc cgcccggcaa gatcgtcggc ggcgaggtcc tgttcatggg
     5401 caaggacctc atcaagatgc ccgtgagcga gctccgctcc atccgcggca acaagatctc
     5461 catgatcttc caggacccga tgacgagcct caaccccttc ctccgcatct ccacccagat
     5521 ggtggagacc atcgtgctcc accaggggct cgacaagaag gcggcgaagg agaaggcgat
     5581 cgagatgctc aagctcgcgg gcatccccgc ccccgagaag cgcatcgacc agtacccgca
     5641 ccagttctcc ggcggcatgc gccagcgcgt catgatcgcg atgggcctct cctgcaaccc
     5701 ggagatcctc atcgccgacg agccgacgag cgccctggac gtcaccatcc aggcgcagat
     5761 cctcgacctc atgaaggacc tgaccgcccg gctcgggacg gccgtcatcc tcatcaccca
     5821 ctcgctgggc gtcgtggccg gcatgtgcga cacgctctgc gtcatgtacg cgggccgcgt
     5881 cgtcgagcgc ggactcacgt ccgagatctt cgccgagccc aagcacccct acacccaggg
     5941 gctcatcaag tcggtgcccc gcctggacca ggcctccggc gacaggctga actcgatcag
     6001 gggccagccg cccaacgtca tcgaccttcc cgactgctgc cccttctacc cgcgctgcga
     6061 caaggccatg gacatctgca agcgcaagta cccggcggcg accgagtacg gcaacggccg
     6121 cgcggtctcg tgctggctct acacggaggc taagtgacca tgaccgagaa cgaagtactc
     6181 ctcgacgtca aggacctcaa gatgcatttc ccgatcggct ccaagagcat gatcgggccg
     6241 cacaagaact tcatctacgc cgtcgacggc gtggacttca agatcaagaa gggcgagacc
     6301 ctgggcctgg tcggcgagtc cggctgcggc aagtccacga ccgcccgcgc cgtcgcccag
     6361 ctctacaagc ccacggccgg cgaggtcatc ctcaagggga aggacctcac caagctcaag
     6421 gccaacgacc tcatcgccgc gcggaagaac atgcagatgg tcttccagga tccctacgcc
     6481 tcgctgaacc cccgcatgac gagcggcgac atcatcgccg agcccatgcg gatctaccag
     6541 atgaagggca tcctccagat gacgaagaag gagatggacg agcgggtcga gcagctcatg
     6601 gagcgcgtcg gcctctcccg cttcttccgc aaccgctacc cgcacgagtt ctcgggcgga
     6661 cagcggcagc gcatcggcat cgcgcgcgcc ctggccctcc ggcccgagct cgtcctctgc
     6721 gacgagcccg tctccgcgct cgacgtctcc atccaggcgc agatcctgaa cctcttcaag
     6781 gacctgcagg acgagttcgg cctgacctac ctcttcatcg cccacgacct ggccgtcatc
     6841 cagtacatct cgacgcgcgt cgccgtcatg tacctgggca cggtcgcgga gatctccggc
     6901 tcggccgagc tctacaagaa gccgctccat ccctacaccc aggccctgct ctccgcggct
     6961 cccatccccg atcccaagat cgagaaggag cgcaagcgca tcatcctcac gggcgacgtg
     7021 ccctctccgg acaagcagcg cccgggctgc cccttctacg accgctgctg gaagcacatg
     7081 gacaagtgca aggaccacaa gccccgcctc cgcgaggccg ctcccggcca cgaggtcgcc
     7141 tgcttcctgt accacgaccc ggactgaaga atcgcctagc gcgaaacgag aaggccgctc
     7201 cccaaaaggg ggcggccttt cttttggagt ggaatccgcg cgggcgcgga aggccggcgg
     7261 ggcggcgcgg cggggcagcg cggcgcggcg gcgcggcgag acggcgggcg tacgggccga
     7321 cgagcacccg cgatgtacgc cgacgcctgg gtcgcccgcg gatcctagtc gtcgccctcg
     7381 gagaagggtt tgcccgcggc cgcggcgagg cggcggccga cctcgcggag ccgctccacg
     7441 cggctcgcgg ggaggatgtt gaggacgagg ccgcccccct ctaggtcgtc gcagacgagg
     7501 ctcacgtagg tcttcttgtg gcggcggcgc cccgacttgg cctccgcgga ggcgtctccg
     7561 cggtcgtcgg cggaggcgag ggcgtcgcgc ttgatcgcgg cctcctcgcc cgagccgggc
     7621 accgagcccc gcacgaagga gcggaggcgg aaggcctcga tccggtcgaa ggggagggcg
     7681 acgctcctcg cgaggacgag gaggccgaag cggtggcgga ggaggccggc gcccgggtcg
     7741 aggatccagc gctcctcgta gagggcggcc aggacggcga gggccagggc tatccagccc
     7801 agggggccgg gggcgccgcc cgaggcgaag gcggcggcca cgaggatccc ggccatgacg
     7861 atcatcgaga gccggtacca gagcggaatg gaatagacga gccgtccctc gcggccggac
     7921 ttgaggacga ggggaatatc catgcagagc ctcctccggg cgccttggcc ccgacagcct
     7981 accatagccc cgccgcggcc cctgggcaag ggccgcgacg gcttgggcgg gccggcgtgg
     8041 gccgcacggg cttggttcgc gccggcttgg gagggccggc gtgggccggg ccgtcttgat
     8101 gcctcccggg gccgcgtcgt atgcttcgac cttccggagg tacctacatg gccaattcgc
     8161 gcgtccagtt cgagacgagc ctcggctccc tcgtcatcga gctcttcgag gattcctgcc
     8221 ccgtcacgac cgccaatttc ctggactatg tccgctcggg cttctacgac ggcaccatct
     8281 tccaccgggt catccccggc ttcgtggtcc agggcggcgg catgctgccc ggcctcaagg
     8341 agaaggacaa ggggaaggct ccgatcaaga acgaggccgc caaggggccc gcgaactccc
     8401 gcggcaccct ctccatggcc aggacctccg atcccgacag cgccacctcg cagttcttca
     8461 tcaacctcgt ggacaacaag tccctggact actccggccc cggcccccgc ggcgccggct
     8521 acgccgtctt cggcaaggtc gtggagggca tggaggccgt cgactcgatg gccaaagtcg
     8581 ccaccggctc ccgcttcccc caccaggacg tgcccaagga ggacatcgtc ctcgtgaagg
     8641 ccaccgtcct ctaacggctt cccttcctag gggctccgcc tcgcctcccg ctccgcggcg
     8701 aggcggggca ggatccccga attccgcctg taggccacca tctcgcagag cgcggcgacc
     8761 gctatctcgg cgggcgtctc cgcgtcgacg tcgaggccca ggggggcgca gaccgcgtcc
     8821 acgcgcgcgg ggtcgaagcc ctcggccttg gccgcgtcca ggaggacgcg cgtcttcctg
     8881 gcgctgccga tcatgccggc gtagcggtac tcccgcggca ggacggccct gaggcagtcg
     8941 aggtcggaca ggtggccgtg ggtgaggatg acgacgtagg tcgcggggtc gaaggagaag
     9001 gccgcgatgg tcttggcgaa gggtcctgga aggggccgga cctccggggg gagctggccc
     9061 gggaggggga agccctcccg gtcgtcggcg acggagactt cgaagccgag aggcagggcg
     9121 gccgccgcca gggcgcggcc gacgtggccg ccgcccagga cgaggagctt ctccctgggg
     9181 aagagcgggt cgtagaagac gaggtccttc tcagagaagc gggcggcgcc cttcccgagc
     9241 gccgcttcgg ccgcccgcgg gtcgatgccc gacagggagc cgaagacggc cttgccatcc
     9301 tcggagagga cggagagctc gacggcgtag ccttcgccgc gcggagaaag gcgtttgacg
     9361 aggagggccc tgcggcccga ggcgaggagg cgcgcggcct cagcgtaggc cggagcctgg
     9421 gagggctcgg ggcgctcgac gaggatgcgg cagagtccgc cgcagatgag ggcggcgccc
     9481 gtcgcgacgg agcccagcat ctccacctcg atgagggagg ccccgcccgc ggcgagccgg
     9541 cgcccggcct cctcgagggc caagagctct cccttgccgc cgccgaccgt gccgacgaag
     9601 tcggcggggc cccgggccag gagcttcgtg ccgggatggc ggggggccga gccctggacg
     9661 gagaggacgg tcgcgagcgc gagggagcct tcggccccgg ccagggcctc gatgatgcgt
     9721 gaatccatgt cgacgctccg ggaggagggc tactcgggct tggccttagg cttgcccctg
     9781 gggcggacct cgggcacctc gtagccggcg agctcgagca tccgcttgaa gggcgaagcc
     9841 tctttcttga gctcgcggga gccgcgggtg atcttgcaga gcgagagccc gagctccttg
     9901 gctatctcgc gctgggggat gcccgcggcg agatccttca cgagggccca gcgcttggct
     9961 atctcgtcgg cctcggaggg ggtgaagagg ctgaagagga agccctgcac gagggtcggg
    10021 tcgtccactt tggcgagggt gcgggacatc tcggcgatgt tctccgacac gagcgtttca
    10081 tctactgcca tgacgtttca tattatacag gctattcccg gaaacggcga gtccaaaaga
    10141 gcgtttcttc aaaggatagt gcggatgccc aaaggcttca ccagggcggc cgggccgggc
    10201 tatgctcgag gccatgctga cttttctccg gcggacgcgc ctggccacga tagtcgccac
    10261 cttggtcgtc ctggccgccc tcctctggcc gggagaggac ctgcccaagg tcgggctcgc
    10321 cggcctggac aaggccgccc acctcctcct cttcggagcc tgggccctgg ccctgcgctt
    10381 cgactggccc ggcctccgcg ccaggccctg gctcctcgtc ctggccgtcg ccctcttcgc
    10441 ccccctcacg gaagggctcc agctcctcgc gccgcagcgg tccttcgacc tcctggacgc
    10501 ggcggccgac ctctgcgggg ctgtcctggc ggcggccttc ggggcgaggc ccgtcgccct
    10561 cgcggagcgg ctcctctcgc cccgaaaggc gcgttagtcg aagacgccgt gcctgtgggg
    10621 cagccaggcg gggtagccga ccgccagggt cgcggccacg gcggcggccg cgtccgcgcg
    10681 gccccaggtg cgcccgcgcc tcatggccct gagccgcctg gccctgaggc tcgcgggcag
    10741 ctcgcaggcg tggagggcct ccacggccag gaacatgccg agccaggcgg ccggggcgag
    10801 ccagagtcct gcgcccgcgg cgcccgccgc ggcgagggcg aggggcgcga gggtggccgt
    10861 ccccgtgaga agggccttcg ccctccagag cagccgctcc ttcacgcgga agacgaggac
    10921 gccgcggaag gcggccaggg ccgcgtcgtc ctcgaaggcg aggaggcgcc tcgcctcctc
    10981 cgccccgagg gggggcaggg gccagggcag ccagtcgcgg ccctcctccc cggccgcgag
    11041 ggccctgccc gggacgaggc cgtcctcgag gcccaggggc acgtccaggc ggcagccctc
    11101 ctcgatgagg cgctgccggg cccgcctcgc gcccagggtg gcggcgagca tggggaagag
    11161 gttctcgttg cgcaaggaca tgacgcggca aggccttccc acgagctcgt ggaggaccat
    11221 gtgcggagcg ttgggcaggg gcatgcatcc gtccacgaag cgttcggcga gacgggccgc
    11281 ggcgcggccg caggaaccga tgacggcctt gcggcccggc agggccgcga tcgcggcctc
    11341 gtcgtaggct ttgccctcgg cgtcgtacca gcggcggcct tgcgccgtcg ggacgccggc
    11401 gcccaggacg aggacggcgc ggcgcctggg gcggatgccc tcgtgctcga gcatggagaa
    11461 ggcgaagcgc gtcgtcgcga cgcagccgcc gcggcaggag gcctcgaggc cgcggacgaa
    11521 ggccggggcg gccgcgccgc cgccgggcag gccgccctcg ccgagagcgc cgccctccgc
    11581 gtcgctggcg ccgcccgcgc cgcaagcgcc gccgcactgg ccgccgccgg ccgcggccag
    11641 gcccttgtcg atgccgatga ggaccaggac ggaggggaag cccttcctca cgcgctcgga
    11701 gtggagggac cagtcggccg gcctgaaggc gacggggact gcgcctccta ggccgtcgcc
    11761 gtccggcgcg caggcgtttg gcgcggcccc gcccgggcct gcgtcgcccg gcgcggcgcc
    11821 cgcggggagc ccgaagccgc gggccaggac gcgcgcgccc tcgggatcgc cgaagccgag
    11881 ctcgtcggcg acgcgcatga gctcgatccg cgaggggtcg tggcccatga gccgcgttgc
    11941 gacgcggtcc gtctccacgg gatggtcgcc cgcgatgacc atgcgggagt cgacgggatc
    12001 cacgggcgcg gggcactcgc cctcgccgcc gacgacgccg tcgacgagga cgaggtcggg
    12061 cttgaagagg tagagcatct ccacgagctt gcggtcgatc gcgtagtggt ggttccgctg
    12121 cctgaggttg tagggtatga cgcccatcgc gttcttgaag ccgagggtca cgcccgtgta
    12181 gaggttcgtc ttgagcttgg gcagggagac gtaggcggct tcgccccgca cgacggcgga
    12241 gaaggggcgg gggacgagga tctcgcgctg gacgcgggcc ttggggagga ggtagcggtc
    12301 gacgggcgtc tcctccaggg cgacgagctt cgcgccgcgg cgtcgcgcca ggcggtcgac
    12361 gccggcgacc ctgaaggagc cgcgggtcgg cgccccccgc cccgaggact cgacgatcgt
    12421 caggctggag gcgcgctcgc cgagccagag cagggccgcg tcgaggaccc gcggatcggt
    12481 gctctcgggg taggtcctcc tcgcgagccc catacggtgg tagaccgtga cgaggttggg
    12541 cttcacgagg acgggccggc ccttgagctt cccgcccagg ccggtctccg cctccagtcg
    12601 gtccagggcg gcgaagacgg cccgcctgat ctcggcgacc tcgggcctcg actcccaggc
    12661 ggccgtgccg tagtcgcggg tcagggccgc gcagtcctcg gcgtagcctc cgggcgcccg
    12721 ggacggagaa agcttctcga cgacgacgac tgccatgctg cctcctgggt gaatgctacg
    12781 gttttgcggc cgggcctgcc cccggcgcgc ggtccgcggc cggggcggcc gagtccgcgc
    12841 ctgcgcccgc gggggcggcc ctgtccgcgg ccggactccc gccgccggcc gccggttgcc
    12901 caagcgccgc ggttcccgcg gccgcgcctt cgccggcgac cgttcgccgc ggcgctcccg
    12961 cgccggcggc caggccggcc aggccgagcc tctcgagggt ctcaggagag ggccggccgc
    13021 ttggccagcc gatctccttc cagtagtcct ccacgaggcg cggaagggga acgctccttc
    13081 ccgcgttggc gccttccgcc ttgggcggaa ggcccagggc gcgcttggag ggcatgaggc
    13141 tctgcgggtc tatcccctcg cgcagggaga agagctggcg cagggtctgg atcctgaggc
    13201 ccgcctcgac gtagtcctcc ggggagcgcg cgaggcccgt cgccgcgtcc aggctctcga
    13261 agaggcccag gcggtcgacg ccgtagaggg agccgaagag gcagaggccg gccgcgttga
    13321 agagggagac gagggcggag gagaggacgg ccttgcgcgc cgcgaggggg ctcgcctcgt
    13381 actcctccga cttccgcgag aggaggggcg ggcggggcgc gccgggcagc ctcttccaga
    13441 gccgccacat cccgtagtag aggtggcagc ccacggtgtg cttccccggc gaagggtcga
    13501 cggcggcgtg gagggcgaag ccggggtcgt tcctgggatc gtgcatcgcg agctcctggc
    13561 ccccggcgtg gacggcaagc ccggccgcgg cggggccgag gcgctcggcg gccttcttca
    13621 cgccgtcggc gaagatcgcg cccaggccct cgcggtccag catctggccg aggaggagct
    13681 ccaccgcctg ggcgttcccc cagcggagct cgaggccgcc ggggagctcc cgcgggacga
    13741 ggccggcctc cgcgcactcg agggccatcg cgaggcaggc gccggcggag atcgagtcca
    13801 tgccggcgcg gttgaggcgc tcgttcatcc ggaagaccgc gggaaggtcg tcgcagagga
    13861 ggaggcccga gagggccagg atcgtctcgt actccggctt gtgggtctcc ttgccctcgt
    13921 gctcgcagat gccgccgcag cccgtcgggc aggagtagca gtggtaggcg cgcttctgcg
    13981 ccgcgaggac caggtcggcg tcgatcctgc gcgacttggc cgcgggatag tccttctccg
    14041 agcccgacca gttgcggatg ggcgagtctc cccactccac ggagaggcgg ttcatgcccg
    14101 cggtgcccca cttggagagg acctccccga agaggaagcc gtcgaagcgc agggcgaggg
    14161 ggagcttgcg gagcagcgcc ccgccctggc cgaggacgga ggccggcggg agggggaggc
    14221 cgcgggggag gcgcctcgcg aaggcggcgg agcgggcgcg gacgccgtcc ctgtcggcgc
    14281 aggggatggg ccgcgtcccg gcggcgacga gggccttgag gcgcttcgag cccatgacgg
    14341 cgccgacgcc cgagcgggcc gctatcctgc cgccgtcgga gacgatgccc gcgatgaggg
    14401 agagggactc gcccgcgcgg ccgatcgcgg cgacctctgg ccgcctgccg ggccgcttct
    14461 tccggagctc ggcctggagc ctctcctccg tcgccaccgc gtccaggccc cagagggcgg
    14521 aggcgtcgcg gagctccgcc ttgccgttcc ccaggtagag gtagacgggc ttctcggaga
    14581 cgccgctcac gaagacgccg tcgtagcccg agcgcttcag ggccggcgcg aggaagcctc
    14641 cgcagttggc gtcgccccag ccgcccgtga ggggggactt gcagacggcc atccagcggc
    14701 cggtgtagag gctgcccgag cccgtgagga ggccgggcac gaagcccagg acggcctcgg
    14761 ggcccagggg gtcggcgccc gcgggcatgc gcgtatagag ccaccaggcc ccgaggccca
    14821 tgccgcccag gaagtcccgg tagacctcct ccggcacctc gaccgcttcg atcttgcccg
    14881 tcgtgaggtc gacggagagg acccttcccg tggcggaacc cggcatggcg cgcctccttc
    14941 accgagagta tgggatatca agttgcaaaa tgcaaacaga aagactaacc ttgaggacgc
    15001 aggccggcgg ggccagccaa ggaccgcggg tgaaggagga cgaaggtgga caggc
//
LOCUS       DAOOSJ010000028        15053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2687_length_15053_cov_5.910188, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000028 DAOOSJ010000000
VERSION     DAOOSJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_15053_cov_5.910188"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..995
                     /locus_tag="PLG14_02665"
     CDS             225..995
                     /locus_tag="PLG14_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HOX48064.1"
                     /translation="MRQKVERIAESLTRVLSGWDSVECVSLCEQAEVDVLDPYFALVL
                     DVHHRGPVPLPEDRRKAFGDPGAFESAQVQTKDRFFLEGLPVRIEYKSVDQVEDFLER
                     AEDRLWILKNSGTYMFYRLGSAQVLFKRSDWIDKTRRRLAEFPASFWEGLRDSFQSKM
                     EHYLADLGAAAVQDDGFFYLVSLGGFATYAAAVLFMVNRRFEPSHRSIDAHLRALKRV
                     PDDFFGRWETLLRSDMDLSREQKYKVAELIAKSLVSFR"
     gene            992..2728
                     /locus_tag="PLG14_02670"
     CDS             992..2728
                     /locus_tag="PLG14_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48065.1"
                     /translation="MRTVPLSSASGRARRRGAALRVLCAWGLALLGSACEAPSVSAFS
                     WTDRGGKPVAFAAQGRPEAGKEGELSGDQPSRAYLPAQALVAGPGEALELELELVGRE
                     GAAPGPAAVPAPLRLDLRLSSRRDGTEPFLSAGLTLRDPLSFVALPFAEGRALASLEL
                     SLEPGAAASGGAAGQALIKIKSIRHVPLSRGYQSLPAGPRLSPGFSLYSSGSELKALI
                     ERPFEGLGAGAALVVDYGTAPAGSSLRLEAVDAAGAVHPFVARLRSGGARLVLPAPLF
                     PAATASVRLSAPAGFALKAFAASALPPEELELADLGLVLLLPPRDASADYDLYRWDLL
                     PEVLVFDFRDYAVQDRYLKRLAFFVEKQGFKGRLAADAEIEGLHGWNAHDYRTEDLAA
                     FFQAARERAFPLSREERELAGLLSARGLVVEKGGRFEAGKGALISITRESVAYLRRMF
                     LTHESTHALFFADAEYRRFVTEAWASLSREEKWFWKLYFGWMNYDTSNDYLMANEFQA
                     YLAQQPVQKAEEYFTKILPPRLLEKHPELEPRIEAYMEEFGPSFAARAAAVEAFLSAK
                     YGILAGRAYFLQ"
     gene            complement(2844..4607)
                     /locus_tag="PLG14_02675"
     CDS             complement(2844..4607)
                     /locus_tag="PLG14_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOX48066.1"
                     /translation="MSDLVFRRNLLALSPRSPDLAALLDETEPDPRLSFRAARSGALV
                     PVYAKDGREAAFHSLMDPRRDAERLAAAQPPGGFIAALGLGAGYGLAPYLASSATTGI
                     LVVEYSAPLLRAVLEEFDLSELFLDPRFSLLLDPEPREIEAALLASYIPPLAGDLRSV
                     PLRGRVDQDPGLFAGAAAAIRKVLDRISDDYSVQAFFGKLWFRNAVRNLFAAERPASP
                     LPPIRRAVVTAAGPSLEAALPAVAKARGEGAFLIATDTSLPALLGSGMRPDAVVSIDC
                     QAISYYHFLKGLPAGVPLVLDLASPNRLARLGGELRFFSSGHPFCAYLSSRFRPFPAL
                     DTSGGNVTHAALSLAEALGAESALLAGADFSYPEGKSYARGTYIYDYFDALSGRLSPS
                     ESLFSGFIFRNASVFRETDRTPEGKEYSRFVTKPLVAYREHLERFAAASSLAVRPLRG
                     LGVEIRIPEGQARKRRERAVFAAGHASMSAADFLKRYAADLAALPPPAEPAAAYLGSL
                     APAERDLWTTLLPSASALQRALGLAKAAAEAESGGGGHAAQGAAERAAALAAAGPAAL
                     LESVRAWALDEIAEALEAAGR"
     gene            complement(4604..6166)
                     /locus_tag="PLG14_02680"
     CDS             complement(4604..6166)
                     /locus_tag="PLG14_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOX48067.1"
                     /translation="MELVFEPARNGEPSARLGSLRLHSAYDPGEEARRFLDSSLGGKR
                     PALVVLLGPCLDYLSPLLKARFPRAAILSIQYSSGFEGRGAADAAWSPGPGPELGDFL
                     ERSLDEDLAVGVTVLEWPAASRAFPAEAEAAARILRGHLERMAASAATLKSFGRRWFA
                     NACRSFLLAERLLAPTALDGALVVAAAGPSLEEALEDLLPHRGRFRLLAVSSALAALR
                     ARGFEPDLVVATDGGFWSRAHLEPLAERPAALAAPLSALASASLSANPLLPVRQDGFP
                     ERELCEALGGGLAIPSHGTVSGTALRLALRLGPGPVIAAGFDFAALGSRSHAGPHGFD
                     HLTRHAASRLGPAEGLAWEREASLSPTLLGEGPWRSSRSLETYAAALSSECESLPDRL
                     FRLRPSPVPLSGFAEIGGKDLPGLLGPARLEPKAFAEASPPPLESRRAVLKALFAHSR
                     EASRQALLAFRAGDSPAGGRAAELFRALDLPDWAAALRALASGGDPGPAAQALDAGVR
                     AYLDDLEGRLLS"
     gene            complement(6171..>7116)
                     /locus_tag="PLG14_02685"
     CDS             complement(6171..>7116)
                     /locus_tag="PLG14_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48068.1"
                     /translation="AVAPKPAQAAAQAPEPVSAVPGAGVVAAPEAFPFSSEELDQLAV
                     ELERLPAGTDELLAAYARIAEALPLQGLALLLAEEGGVQIAAERGLGAAADSCDGISL
                     EVIAAASEKGSCGPRTRRLLSPLLKLPADRSTRIYSVGEGARAVWLYADARLDASSAA
                     QLSSAESLFASTGGRGTPLPLRGAVVGTKEAAAALEGEAAFAAVILLELKAFIDDFEL
                     LVPGLRRHAIEGALRSAATSLLGANGAAFCLSGERLAAFLYSGIRLDAELALAQFRKS
                     LRRSLPFLGMAELPEGRALGLDLGYEGAAGLLERFLAE"
     assembly_gap    7117..7216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7599..10052)
                     /locus_tag="PLG14_02690"
     CDS             complement(7599..10052)
                     /locus_tag="PLG14_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48069.1"
                     /translation="MAARPDNDMRKAAPRSASPQGELEQYGVWVKAEPQDIVEEPEAT
                     AEIEESFDLGLPEESAPLPEEAFLTEDEEELLGSFEELEAEEPAPKAAKAPAESRAAP
                     GPAFPDIEDLPELEDFGIPEEPEPPRAAAPSAARTAKPAAFEAEPVEDFGSATIDISL
                     DDLETVSREPSPIRPDAEIDISSIEGLEELPEFAPAAKEERASPESASFDIEDVSAEF
                     LDLGGEEKPSAPSGGAHDVSSEFLEAEPQAVEPLEGESADFEPIDIDLHFDDTIPSGG
                     RAGAEEEAGFESVSEFDDFLSEGEAKPAPAAKPAKAAPKAEPGFDDLAALERELAEPA
                     PEAPPSRPAAAAPQSALSNELLVKIADELASIRGELVNLKGQLGSIQREGLAPAPCEP
                     VSAAEAEPKQAGAAAGFFDEEEDETIALTGDELDNILNTADFTEETPAEEAALGEEEI
                     SLHEEGLSEEILLPESGDYAAPEPAIEEIRLEPAIEEEPEAPKGEDLDLVEELLSDGG
                     VTPMTSAPEDTSYLEESLAAEVDLGFAETPLAEAPLVEPDLSELDLETEELEPSVEVT
                     EELPVIEGLQAAAEEEPLEDLTLGIEGSADYLSEQVPEAESLEPLPEIEETSFGEISL
                     AEEGLAELEPELEELAAEEPSLDEEISERTVKPAQPVALNPDQIPLSLDESYFVGSEP
                     AAAEAPLEEAALEEEPLEEISFGETSLEEAPAAAMEPAGAMEPAEEPLPSLESFGVPE
                     AEETLELAAEAEEPLAKAPSPASRAEGGTDRLKGEIRSVLSYLDKLLESLPEDKIEEF
                     ARSEYFDTYKKLFEELGLV"
     gene            10198..10680
                     /locus_tag="PLG14_02695"
     CDS             10198..10680
                     /locus_tag="PLG14_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOX48070.1"
                     /translation="MSSYIARRLGFALYAGLAAYCVLSLTVGPAGLVAYRGLGGRRTA
                     MEGRLAELRAANESLRGDLESLRSDPDRADREARELGYLRPGETFLVIAGRAGAGQGG
                     LPAAGEPLAYVDPPACPDSTLKELSLGLGLAVLIAGLAGDARRLPAQRKSRLQAASRT
                     "
     gene            complement(10641..11627)
                     /locus_tag="PLG14_02700"
     CDS             complement(10641..11627)
                     /locus_tag="PLG14_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48071.1"
                     /translation="MRKTAALFFALALALAGRAAAEQAKPVLRIRPFSGEDLAASELA
                     AIQNLVTSYIVELKAFRVVDSRGQELALQEAETAVSLGAPKDLAPLAADYLVTGSVAK
                     AGGLLVFSLEATKVSTGEKRSVSEVLPSINDLILQSKRLTRALFERTEGEKAAVLPAA
                     GAAPLAPAISPSAADAGPGAAFAPPLPPSDLPPHQAAMASPSLAKAAGTWKGDKGVER
                     VSLFPDGRGIAVLASGATMKVRARLAGGEIEIVQDQPNLPDFYRGPGIDYKAAKVVAA
                     QARPWRWVFRLSEDGSSLFGTKESVFVRIETSGKVLVDNGYVREAAWSRLFR"
     gene            complement(11702..14347)
                     /locus_tag="PLG14_02705"
     CDS             complement(11702..14347)
                     /locus_tag="PLG14_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOX48072.1"
                     /translation="MSAPFRPAKAAKTLVIVAACFALLLGIAAGAAIAATRNIRATED
                     FTDFQPNLPTKILDVKGRLITEFSSDEKREMVSIKDLPRHLIDALVTREDQSFYRHRG
                     FTIKSISRAFVGKLVGKSLGGGSTITQQVAGTLYADRSDYSIRRKLVELWWALQLERR
                     YTKEEILEIYLNRMYMGAGCYGVEAASKFYFGHSAREIGLAESAVLVIQLSSPSKYNP
                     IENPNNARARSREVLDQMARLGYCSKADADAAFDEYWDNFDYTRVATSAFFMRDDKAP
                     WFSEYVRRQLEDMLYGSLDIYKDGFVVNTTLDLDMQAAADLYMKRGIDQVNKEFKASS
                     GLRLSEAEKTYVPIVEMLGLAFDFEELFVSEAKVKGKTLDYFQRRLNPAIDAAALLFG
                     LSDLKAIANASSGKAKTDLEKTTVEGALVTLENETGHVKAIVGGSKFDQSNQLIRATQ
                     ALLMPGSCFKPLYYSAAIDTRKFTEGSLIYDSPVVFYNEDGTPYIPLNYKGEWKGPVL
                     TWYALAKSMNVPSLKVLDSIGFDAAIDRAASLLDIRDPAEVRRTFPRVYPLGLGVIGV
                     SPIKMARAFAVFGNQGKEVTPIAIRSVEDRNGRIILEPEKDLRTQQKKKGQGIQVVSP
                     QNAAVMVDMLERVVQYGTLQGPSGGGAALSYKGEDGKKYTIPSAGKTGTTQNWADAWT
                     VGFTPYMTTAIWFGFDRPGNSLGVNQSGATIAGDYWSQYMREIHRGLPYKDFARPQTG
                     LVQVSVCSVSGLLPTEFCDEGTTSLLFYEGTEPKKYCDLHQYGAERDRSLLDKLGDQM
                     KLYGDSGAVDSKLNLDIPGLDLGAPSAPPPSVPLPGAATPLPGAASPLPGALAPAAAP
                     SGGSPAGAVPAAPTPAEGAATGLLD"
     gene            14762..>15053
                     /locus_tag="PLG14_02710"
     CDS             14762..>15053
                     /locus_tag="PLG14_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOX48073.1"
                     /translation="MKVAINGFGRIGRLVFQAIVDQGLLGKDKIDVVAVVDISTDAEY
                     FAYQLKYDSVQGRMKADISAKKSDASLAEADVLVVNGHEVKCVMAEKEVKNLP"
BASE COUNT         2185 a         4922 c         5618 g         2228 t
ORIGIN      
        1 tcggcggagc ggaggcggag gccgaaggcc aggggggccg cgtcggggaa ggccagggcc
       61 gaggcgtccc cgagggcgga gaagatcctc cgcctgtaga gggagagctc cctggcctcg
      121 ttgccgggag gctccaggac gagggcgtag agcgcccctc ttgtttccaa agccatcggg
      181 ccatggtaca attatcccga gatccctgta aaggaaggcg gcacatgaga cagaaagtgg
      241 agcgcatcgc cgaaagcctc acgcgggtcc tctcgggctg ggacagcgtg gagtgcgtga
      301 gcctctgcga gcaggcggag gtcgatgtcc tcgatcccta cttcgccctc gtcctcgacg
      361 tgcaccaccg cggccccgtg cccctgcccg aggaccgaag gaaggccttc ggggatccgg
      421 gcgccttcga gagcgcccag gtccagacga aggacaggtt cttcctggag gggctccccg
      481 tccgcatcga gtacaagagc gtggaccagg tggaggattt cctggagcgc gcggaggacc
      541 gcctctggat cctcaagaac tccggcacct acatgttcta ccgcctcggg agcgcccagg
      601 tcctcttcaa gcgctcggac tggatcgaca agacccgccg ccgcctcgcg gaattccccg
      661 cctccttctg ggagggcctc cgggacagct tccagtccaa gatggagcac tacctcgccg
      721 acctgggcgc cgcggccgtc caggacgacg gcttcttcta cctcgtctcc ctcggcggct
      781 tcgcgaccta cgcggcggcc gtcctcttca tggtgaaccg ccgcttcgag ccctcgcacc
      841 gctccatcga cgcccacttg cgcgccctga agcgagtccc cgacgacttc ttcggccgct
      901 gggagaccct cctgcgctcc gacatggact tgagccgcga gcagaagtac aaggtggccg
      961 agctgatcgc caagagcctc gtctccttca gatgaggacc gtccccctgt cctccgcctc
     1021 cggccgcgcg cgccgccgcg gcgcggccct gcgcgtcctt tgcgcctggg gcctcgccct
     1081 cctcggctcc gcctgcgagg cgccgtcggt gtcggccttc tcctggaccg accgcggggg
     1141 aaagcccgtc gccttcgccg cccaagggcg tccggaggct gggaaggagg gggagctctc
     1201 cggcgaccag ccctcgcggg cctacctccc ggcccaggcc ctcgtggccg gccccggcga
     1261 ggccctcgaa ctcgagcttg agctcgtcgg gcgcgaggga gccgctccgg gcccggcggc
     1321 ggttccggcg cctctccgcc tggacctgcg cctctcctcc cgccgcgacg ggaccgagcc
     1381 cttcctctcc gcgggcctga ccctccgcga cccactctcc ttcgtcgcgc ttcccttcgc
     1441 cgaggggagg gccctggcca gcctcgagct ctccctcgag ccgggcgccg ccgcctcggg
     1501 cggggccgcg ggccaggctt tgatcaagat caagtcgatc cgccacgtcc cgctttccag
     1561 gggctaccag agcctccccg cggggccccg cctctcgccc ggcttttccc tgtactcgag
     1621 cggctcggag ctcaaggccc tcatcgagag gcccttcgag ggactgggcg cgggagccgc
     1681 cctggtcgtc gactacggca cggccccggc gggctcttcc ctccgcctgg aggccgtcga
     1741 cgccgcgggc gccgtccatc ccttcgtcgc gcggctccgc tcgggcggcg cccgcctcgt
     1801 cctccccgct ccgctcttcc cggcggccac ggcgagcgtg aggctgtcgg cccccgcggg
     1861 cttcgccctc aaggccttcg ccgcctcggc cctgccgccc gaggagctcg agctcgccga
     1921 cctcggcctc gtcctcctcc tgccgccgcg ggacgccagc gccgactacg acctctatcg
     1981 ctgggatctc cttcccgagg tcctcgtctt cgacttcagg gactacgccg tccaggaccg
     2041 ctacctgaag cgcctcgcct tcttcgtcga gaagcagggc ttcaagggaa ggctcgccgc
     2101 cgacgcggag atcgaggggc tccacggctg gaacgcccac gactaccgga ccgaggacct
     2161 ggccgccttc ttccaggccg ccagggagag ggccttcccc ttgagccgcg aggagaggga
     2221 gctcgccggc ctcctttcgg cccgcggcct cgtcgtcgag aagggaggcc ggttcgaggc
     2281 ggggaagggc gccctcatct cgatcacgag ggagtcggtc gcctacctcc gccgcatgtt
     2341 cctcacccac gagtccaccc acgcgctctt cttcgcggac gcggagtacc gccgcttcgt
     2401 caccgaggcc tgggcctcgc tgtcgcgcga ggagaagtgg ttctggaagc tctacttcgg
     2461 ctggatgaac tacgacacct cgaacgacta cctgatggcg aacgagttcc aggcctacct
     2521 cgcccagcag ccggtccaga aggccgagga gtacttcacg aagatcctgc ctccgcgcct
     2581 cctggagaag catcccgagc ttgagccgcg catcgaggcc tacatggagg agttcgggcc
     2641 gagcttcgcg gcccgcgccg ccgccgtcga ggccttcctc tccgccaagt acggcatcct
     2701 cgcgggccgc gcctacttcc tgcagtaagc gagccgagga tctaccgatc cgcggctaac
     2761 aagcgggcag cggacctaac ggcccgctgc caacaagcga gccgcggacc taccggtccg
     2821 cggctaacaa gagggccgcg ggcctagcgg cccgcggcct caagggcctc ggcgatctcg
     2881 tccaaggccc aggcgcggac gctctcgagg agggcggccg ggcccgccgc ggccagggcc
     2941 gcggcccgct cggccgctcc ctgggccgca tgcccgccgc cgcccgactc ggcctccgcg
     3001 gcggccttcg cgaggcccag ggcccgctgc agggcgctcg cgctcgggag gagggtcgtc
     3061 cagaggtccc gctcggccgg ggccagggag cccaggtagg ccgcggcggg ctcggcggga
     3121 ggcggcaggg cggccaggtc ggccgcataa cgcttaagga agtccgccgc gctcatggag
     3181 gcgtggccgg cggcgaagac ggcgcgctcg cgccgcttcc tcgcctgccc ctcggggatt
     3241 cttatctcga cgcccaggcc gcgcaggggc ctcaccgcca gggacgaggc ggcggcgaag
     3301 cgctccaggt gctcgcggta ggcgaccagg ggcttggtca cgaagcgcga gtactccttg
     3361 ccctcgggcg tcctgtccgt ctccctgaag acggaggcgt tgcggaagat gaagccggag
     3421 aagaggctct ccgagggcga gagcctgccc gacagggcgt cgaagtagtc gtagatgtag
     3481 gtgccccgcg cgtagctctt gccctcgggg taggagaagt ccgctccggc gaggagggcc
     3541 gactcggcgc ccagggcctc cgccagggag agggccgcgt gggtgacgtt gccgcccgag
     3601 gtgtccaggg cggggaaggg cctgaagcgc gaggagaggt aggcgcagaa ggggtggccc
     3661 gaggagaaga agcggagctc cccgcccagc ctcgcgagcc tgttgggcga ggcgaggtcg
     3721 aggacgaggg ggacgcctgc cggcaggccc ttgaggaagt ggtagtagct gatggcctgg
     3781 cagtcgatgg agacgacggc gtcggggcgc atccccgagc cgaggagggc cggcaggctc
     3841 gtgtcggtcg cgatcaggaa ggcgccctcg cccctggcct tggccaccgc cggcagggcg
     3901 gcctccaggg aagggccggc ggcggtgacg acggcccgcc ggatcggcgg caggggcgag
     3961 gccggcctct ccgcggcgaa gaggttccgc accgcgttcc tgaaccagag cttcccgaag
     4021 aaggcctgga cggagtagtc gtcggagatc ctgtccagca ccttccggat cgcggccgcg
     4081 gcgccggcga agaggccggg atcctggtcg acgcggccgc ggaggggcac gctccggagg
     4141 tcgcccgcga ggggcggaat gtaggaggcg aggagggcgg cctcgatctc gcgaggctcg
     4201 ggatcgagga ggaggctgaa gcggggatcg aggaagagtt cggagaggtc gaactcctcc
     4261 aggacggcgc ggagtagggg ggccgagtac tctacgacga ggatgcccgt ggtcgcgctc
     4321 gaggcgaggt agggcgcgag gccgtagccg gccccgaggc ccagggccgc gatgaagccc
     4381 ccgggcggct gggccgccgc gaggcgctcc gcgtcgcggc gcggatccat gagggagtgg
     4441 aaggcggcct cgcggccgtc cttcgcgtag accggcacca gggcgccgga gcgggccgcc
     4501 ctgaaggaga gccgagggtc gggctcggtc tcgtccagga gggcggcgag gtcgggcgac
     4561 ctcggggaga gggccaggag attgcgcctg aacacaaggt cgctcacgag aggagcctcc
     4621 cttcgaggtc gtcgagatag gcccggacgc ccgcgtccag ggcttgggcg gccggcccgg
     4681 gatcgccgcc cgaggccagg gcgcgcaggg cggcggccca gtcgggaagg tccagggcgc
     4741 ggaagagctc ggcggcgcgg ccgcccgcgg gggagtcgcc ggcccggaag gcgagcaagg
     4801 cctggcggga ggcctcgcgc gaatgcgcga agagcgcctt gaggacggct ctcctcgatt
     4861 cgagcggagg cggggaggcc tcggcgaagg ccttcggctc gagcctcgcg ggacccagga
     4921 gccccgggag gtccttccct ccgatctcgg cgaagccaga tagggggacg ggcgagggcc
     4981 tgagcctgaa cagcctgtcc gggaggcttt cgcattcgga ggagagggcc gccgcgtagg
     5041 tctcgaggct ccgcgagctt ctccaggggc cctcgccaag aagcgtcggg gagagggagg
     5101 cttccctctc ccaggcaagg ccctcggcgg gcccgaggcg ggaggcggca tggcgcgtga
     5161 ggtggtcgaa gccatggggg cccgcgtggg atcgggagcc cagggcggcg aaatcgaaac
     5221 cggcggcgat gaccggcccg gggcccaggc gcagggccag gcgcagggcc gtgcccgaga
     5281 ccgtgccgtg gctcggaatg gccaggcccc ctcccagggc ctcgcagagc tcccgctcgg
     5341 ggaagccgtc ctgcctgacg gggaggaggg gattggcgga gagactcgcc gaggccaggg
     5401 ccgagagggg agcggcgagg gcggcgggcc tttccgccag gggctccaga tgggccctgc
     5461 tccagaagcc gccgtccgtc gcgaccacga ggtcgggctc gaagcccctg gccctgaggg
     5521 ccgcgagggc cgaggagacc gcgaggagcc ggaagcggcc gcgatggggc agcaggtcct
     5581 ccagggcctc ctccaggctc gggccggcgg ccgcgacgac cagggccccg tccagggccg
     5641 tcggcgcaag gaggcgctcg gcgaggagga aggagcggca ggcgttggcg aaccatctcc
     5701 tgccgaagct cttgagggtc gcggcggagg cggccatccg ctccaggtgt ccgcggagga
     5761 tccgggccgc cgcctccgcc tcggcgggaa aggcccggga cgcggccggc cactcgagga
     5821 cggtcacgcc gacggccagg tcctcgtcca ggctcctttc caggaagtcg cccaattccg
     5881 gtccggggcc cgggctccag gcggcgtcgg ccgcgccccg tccctcgaag ccggacgaat
     5941 attggatgga gaggatggcg gcgcgcggga aacgggcctt gaggaggggg gagagatagt
     6001 ccaggcaggg gccgagaagg accacgaggg cgggacgctt cccgcccagc gaggagtcca
     6061 ggaagcgcct ggcctcctct ccggggtcgt aggcggagtg gagccgcaag gagcccaggc
     6121 gggcgctcgg ctcgccgttc cgcgcgggct cgaagacgag ctccacggtc ctactcggcg
     6181 aggaagcgct caaggaggcc ggcggccccc tcgtagccca ggtccaggcc gagggcgcgg
     6241 ccctcgggga gctcggccat gccgaggaag ggcagggatc gtcggaggga cttgcggaac
     6301 tgggccaggg cgagctcggc gtccaggcgg atgccggagt agaggaaggc ggcgaggcgc
     6361 tcgcccgaga ggcagaaggc cgcgccgttg gctccgagga gggacgttgc cgcgctccgc
     6421 agggcgccct cgatcgcgtg gcggcggagg cctggcacga ggagttcgaa gtcgtcgatg
     6481 aaggccttga gctcgaggag gatcaccgcg gcgaaggccg cctcgccctc gagggccgcg
     6541 gccgcctcct tggtcccgac gacggcgccg cgcaggggca ggggcgtgcc tcggcctccc
     6601 gtggaggcga agagggactc ggccgaggac agctgcgcgg ccgacgaggc gtccaggcgg
     6661 gcgtcggcgt agagccagac ggcgcgcgcg ccttcgccga cggagtagat cctggtcgaa
     6721 cggtcggcgg gcagcttgag gaggggcgaa aggagccgcc gcgtcctggg gccgcagctg
     6781 cccttctccg aggccgccgc gatgacctcc agggagattc cgtcgcagga gtcggcggca
     6841 gcgcccaggc cgcgctcggc ggcgatctgc acgccgccct cctcggccag gaggagggcg
     6901 aggccctgga ggggcagggc ctccgcaatc ctggcgtagg cggcgagcag ctcgtccgtc
     6961 cccgcgggaa ggcgctccag ctccacggcc agctggtcga gctcctcgga agagaagggg
     7021 aaggcctcgg gggcggccac gacgccggcg ccggggacag cggaaacggg ttcgggagct
     7081 tgcgccgcag cttgcgccgg ctttggcgcg acagctnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnggaa acgggttcgg gagcttgcgc cgcagcttgc gccggctttg
     7261 gcgcgacagc tttttgcggg atcggttttg gcgcggttgg agcgggcgcg gcaggagcgg
     7321 gcgcggcggg cttgccggct aggggctcgg gcgggttagc cttggccgcg accggcttgg
     7381 gcgccagggg agccgcggcg accggcttgg cggctagggg agcggcagcg acgggcttgg
     7441 ccgccgcgcg cctggcggag acgggagcag gctcggactg ctccctgtcg gactcgtccg
     7501 aggcctccag ggcgtccaag gcttttgaga aaaggccgga acctgcctcg ggtttcccgg
     7561 ccttgcgttc gcggagcgcc ttttcgaaaa ggcccatctt acaccaagcc caattcctcg
     7621 aagagcttct tgtaggtatc gaagtactcg gatctggcga attcctcgat cttgtcctcg
     7681 ggcagggact caaggagctt gtcgaggtag gagagcacgc tcctgatctc gcccttcagc
     7741 ctgtcggtgc ctccctcggc gcgcgaggcg ggagagggag ccttggcgag aggctcctcc
     7801 gcctcggccg cgagctcgag agtctcctcg gcctcgggga cgccgaagga ctcgagggag
     7861 gggaggggct cctcggcggg ctccatggcc ccggcgggct ccatggccgc ggcgggcgcc
     7921 tcctcaaggg acgtctcgcc gaaggatatc tcctcaaggg gctcctcctc cagggcggcc
     7981 tcctcgaggg gagcctccgc ggcggcgggc tcggagccga cgaaatagct ctcgtcgagg
     8041 ctcaagggga tctggtccgg attgagggcg accggctggg ccggcttgac ggtacgctcg
     8101 gagatctcct cgtccagcga gggctcctcg gcggcaagct cctcgagctc gggctcgagc
     8161 tcggccaggc cctcctctgc gaggctgatc tcgccgaagg aggtctcctc gatctcgggg
     8221 agaggctcga ggctctcggc ctcgggcacc tgctcggaca gatagtcggc agagccctct
     8281 atgcccaggg tgaggtcctc gagcggctcc tcctcggcgg ctgcctggag gccctcgatg
     8341 acgggcagct cctcggtgac ctccacggag ggctcgagct cctcggtctc caggtcgagc
     8401 tccgagaggt cgggctcgac caggggcgcc tcggcgaggg gcgtctcggc gaagccgagg
     8461 tcgacctcgg cggcgagcga ttcctcgaga taggaggtgt cctcaggcgc ggaggtcatg
     8521 ggcgtcaccc cgccatcgga gaggagctcc tcgacgaggt cgagatcctc gcccttggga
     8581 gcctcgggct cttcctctat ggcgggctcg aggcggatct cctcgatcgc cggctcggga
     8641 gccgcgtagt cgccggactc gggaaggagg atctcctcgg agaggccctc ctcgtggagc
     8701 gatatctcct cttcgccgag ggccgcctcc tcggcggggg tctcctcggt gaagtcggcg
     8761 gtattgagga tgttgtcgag ctcgtcgccg gtgagggcga tggtctcgtc ctcctcctcg
     8821 tcgaagaagc cggccgcggc cccggcctgc ttgggctcgg cctcggccgc ggagacgggc
     8881 tcgcagggcg ccggggccag gccctcccgc tggatggagc cgagctggcc cttcaggttg
     8941 acgagctctc ccctgatgga ggcgagctcg tcggcgatct tcacgaggag ctcgttcgag
     9001 agcgccgact ggggggcagc ggccgcgggg cgtgagggcg gggcttcggg ggcgggctcg
     9061 gcgagttcgc gctccagggc ggcgaggtcg tcgaagccgg gctcggcctt gggggcagcc
     9121 ttggcgggct tggcggcagg cgcgggcttg gcctcgcctt cggagaggaa gtcgtcgaat
     9181 tccgagacgg attcgaagcc cgcctcctcc tcggcgccgg cgcggccgcc cgaggggatg
     9241 gtgtcgtcga aatggaggtc gatgtcgatg ggctcgaagt cggcggactc gccctcgagg
     9301 ggctcgacgg cctggggttc ggcctccagg aactcgctcg agacgtcatg ggcgccgcca
     9361 gagggggcgg agggcttctc ctcgccgccc aggtccagga actcggcgga gacgtcctcg
     9421 atgtcgaagg aggcggactc gggggaggcg cgctcctcct tggccgcggg ggcgaattcg
     9481 ggcaactcct caaggccttc gatggaggag atgtcgatct cggcgtccgg cctgatcggg
     9541 gagggctcgc gggagacggt ctccaggtcg tccagggaga tgtcgatggt ggcggatccg
     9601 aagtcctcca cgggctcggc ctcgaaggcg gcgggcttgg ccgtcctggc ggcggaaggc
     9661 gcggccgcgc gcgggggctc gggctcctcg ggaatgccga agtcctcgag ctcgggcagg
     9721 tcctcgatgt cggggaaggc ggggccgggg gccgcgcggg actccgccgg ggccttggca
     9781 gccttgggcg cgggctcctc ggcctcgagc tcctcgaagg agcccaggag ctcctcctcg
     9841 tcctcggtga ggaaggcctc ttcgggcagc ggcgcggatt cctccggcag cccgaggtcg
     9901 aagctctcct cgatctcggc ggtggcctcg ggttcctcga cgatatcctg gggctcagcc
     9961 ttgacccaga caccgtactg ctcgagctct ccctgggggg aggcgctgcg gggcgcggct
    10021 tttctcatat cgttgtcggg acgtgctgcc atggcttctc cgttgcggac ggtcgtatag
    10081 ttgtgtatcg gcaggaatga cccccgcatt aacggttcca tcctactcta agcgctcctc
    10141 aaccggatgt caacaatagg cgtcaaggag gaggccgcgc cttccgtaaa tagcaaggtg
    10201 agcagctaca tcgcgcgcag gctgggcttt gcgctatacg ccggcctcgc ggcctattgc
    10261 gtcctctccc tcacggtcgg gccggccggc cttgtcgcgt accgcggcct gggcgggcgc
    10321 cggaccgcca tggaagggcg cctggccgag ctccgcgcgg ccaacgagag cctgcggggc
    10381 gacctcgagt cgctgcgctc ggaccccgac cgcgcggacc gcgaggcccg cgagctgggc
    10441 tacctcaggc ccggggagac gttcctcgtg atagccggaa gggcgggggc gggccagggc
    10501 ggtctgccgg ccgcgggcga gcccctggcc tacgtcgatc cgcccgcctg ccccgactcg
    10561 acactgaaag agctgtcgct cggcctgggc ctcgcggtcc tcatcgccgg gctcgccggg
    10621 gacgcgaggc gcctacccgc tcagcggaag agccggctcc aggcggcttc ccgcacgtag
    10681 ccgttgtcga ccaggacctt gcccgaggtc tctatccgca cgaagaccga ttccttggtc
    10741 ccgaagaggc tcgagccgtc ctccgaaagc ctgaagaccc agcgccaggg cctggcctgc
    10801 gcggcgacga ccttcgcggc cttgtagtcg atgcccggcc cccggtagaa gtccggcagg
    10861 ttgggctggt cctggacgat ctcgatctca cctcccgcaa ggcgcgccct caccttcatc
    10921 gtcgcgccgg aggccaggac ggcgatgccg cggccgtcgg ggaagaggct cacccgctcc
    10981 acgcccttgt cgcccttcca ggtgccggcg gccttggcca ggctcggcga ggccatcgcg
    11041 gcctggtgag gcggcaggtc tgaggggggg aggggcgggg cgaaggcggc tcccgggccc
    11101 gcgtcggccg cggatgggct gattgccggg gccagggggg cggcgcccgc agcgggcagg
    11161 acggccgcct tctcgccctc cgtccgctcg aaaagggccc tggtcaggcg cttggactgg
    11221 aggatgaggt cgttgatcga ggggaggacc tccgagacgc tccgcttctc ccccgtggag
    11281 accttggtcg cctccaggct gaagacgagg aggccgcccg ccttggccac cgagcccgtc
    11341 accaggtagt ccgccgccag gggagccagg tccttggggg cgcccaggct caccgcggtc
    11401 tccgcctcct ggagggccag ctcctggccc cgcgagtcga ccacgcggaa ggccttgagc
    11461 tcgacgatgt agctcgtgac gaggttctgg atggccgcga gctcggaggc ggccaggtcc
    11521 tcgcccgaga agggccggat ccgcaggacg ggcttcgcct gctctgcggc ggcccggccc
    11581 gccagggcca gggccagggc gaagaagaga gcggcggttt tccgcatgcc agcctccagg
    11641 gaaagtgtat gccgcccgga ccgcccgcgc cagccctctc tttcgagggc cgcggctagt
    11701 gtcagtcgag caggcccgtc gcggcgcctt cggccggggt gggcgcggcc ggaacggcgc
    11761 cagcggggga gccgcccgac ggcgcggcgg ccggggcgag ggcgccggga aggggcgagg
    11821 cagcgccggg aaggggcgtg gccgcgccgg gaagaggcac ggacgggggc ggcgccgaag
    11881 gcgcgccaag gtccaggccc gggatgtcca ggttgagctt ggagtccacc gcgcccgagt
    11941 ctccgtagag cttcatctgg tccccgagct tgtcgaggag gctgcggtcg cgctcggcgc
    12001 cgtactggtg caggtcgcag tacttcttgg gctcggtgcc ctcgtagaag aggaggctcg
    12061 tcgtgccctc gtcgcagaat tcggtcggaa ggaggccgga gacggagcag acactgacct
    12121 ggaccaggcc ggtctggggc cgggcgaagt ccttgtaggg caggccgcga tggatctccc
    12181 gcatgtactg ggaccagtag tcgccggcaa tcgtcgctcc cgactggttc acgcccaggg
    12241 agttgccggg gcggtcaaag ccgaaccaga tcgccgtcgt catgtaaggc gtgaagccga
    12301 ccgtccaggc gtcggcccag ttctgggtcg tgcccgtctt gccggcggag gggatcgtgt
    12361 acttcttgcc gtcctcgccc ttgtaggaga gggcggcgcc gccgcccgag gggccctgga
    12421 gggtgccgta ctggacgacc cgctcgagca tgtcgaccat caccgccgcg ttctgcggcg
    12481 agacgacctg gatgccctgg cccttcttct tttgctgggt gcgcaggtcc ttctcgggct
    12541 ccaggatgat gcggccgttg cggtcctcca cggagcggat cgcgatgggc gtgacctcct
    12601 tgccctggtt gccgaagacg gcgaaggcgc gggccatctt gatcggcgag acgccgatga
    12661 cgcccaggcc cagggggtag acccggggga aggtccgcct gacctcggcc gggtcgcgga
    12721 tgtcgaggag ggaggcggcg cggtcgatgg ccgcgtcgaa gccgatggag tcgaggacct
    12781 tgagggaggg cacgttcatc gacttggcca gggcgtacca ggtgaggacg gggcccttcc
    12841 actcgccctt gtagttgagc gggatgtagg gcgtgccgtc ctcgttgtag aagacgacgg
    12901 ggctgtcgta gatgaggctg ccctcggtga acttccgggt gtcgatggcc gcggaatagt
    12961 agaggggctt gaagcaggag ccgggcatga ggagggcctg ggtcgcgcgg atgagctggt
    13021 tcgactggtc gaacttcgag cctccgacga tggccttcac gtggccggtc tcgttctcca
    13081 gggtgacgag ggcgccctcg accgtggtct tctccaggtc ggtctttgcc ttgcccgagc
    13141 tcgcgttggc gatggccttg aggtcggaga ggccgaagag gagggccgcc gcgtcgatgg
    13201 cggggttcag ccgcctctgg aagtagtcga gggtcttgcc cttgaccttc gcctcggaga
    13261 cgaagagctc ctcgaagtcg aaggccaggc cgagcatctc gacgatgggc acgtaggtct
    13321 tctccgcctc ggagaggcgc aggccgctcg aggccttgaa ttccttgttg acctggtcga
    13381 tgccccgctt catgtagagg tcggccgcgg cctgcatgtc caggtccagg gtcgtgttga
    13441 cgacgaatcc gtccttgtag atgtccaggg agccgtagag catgtcctcg agctggcggc
    13501 ggacgtactc ggagaaccag ggggccttgt cgtcgcgcat gaagaaggcg ctcgtcgcga
    13561 cgcgggtgta atcgaagttg tcccagtact cgtcgaaggc cgcgtcggcg tccgccttgg
    13621 agcagtagcc gagcctggcc atctggtcca ggacctcgcg ggagcgggcc cgcgcgttgt
    13681 tcgggttctc gatgggattg tacttggagg ggctggacag ctggatcacg aggacggccg
    13741 actcggccag gccgatctcg cgggcggagt ggccgaagta gaacttgctc gccgcctcca
    13801 cgccgtagca gccggcgccc atgtacatcc tgttgaggta gatctcgagg atctcctcct
    13861 tcgtgtagcg ccgctccagc tggagggccc accagagctc gacgagcttt cgccggatcg
    13921 aatagtcgga acggtcggcg tagagggtgc cggccacctg ctgggttatc gtcgagccgc
    13981 cgccgaggct tttgccgacg agctttccca cgaaggcgcg ggaaatgctc ttgatggtga
    14041 agccgcggtg ccggtagaag gactggtcct cccgcgtgac aagggcgtcg atgaggtggc
    14101 ggggcaggtc cttgatggag accatctcgc gcttctcgtc ggaggagaac tcggtgatga
    14161 gccggccctt gacgtcgagg atcttcgtcg ggaggttggg ctggaagtcg gtgaagtcct
    14221 cggtggccct gatgttgcgc gtggccgcga tggcggcgcc ggcggctatg ccgaggagga
    14281 gggcgaagca cgcggcgacg atcacgaggg ttttcgcggc cttcgcgggc ctgaatgggg
    14341 cggacatgcc ggctagggta cgcgaaggcc gaggccttgg tcaagttgag cataaaaaaa
    14401 ggccggtttg gggaaaaccg gccggcccgt agcgggtgtc ggaaggaacc atagtaaact
    14461 gggaggggaa ctatgatcga atccgacaac cacaatatcg gcagcgggct gaagtaaatt
    14521 taatgcatcc tggagaaatt tcaaagcggg acgggcctcc aatcgtccgc gggggctgga
    14581 taatcatgcg gagcattgcg tatattctta cttcggacgt cggggcgccg gaatcttgcc
    14641 caaccctcgc gcgagaggcg ttgacaagcg tttcacgctt cgatatgatt ttgatataaa
    14701 aatatagaaa ctatcgcgat atgcgatccg ctttcgcgat attccacacg ggaggaacga
    14761 gatgaaagtt gccatcaacg gtttcggcag gatcggccgc ctggtcttcc aggccatcgt
    14821 cgaccagggg ctcctcggca aggacaagat cgacgtcgtc gccgtcgtcg acatctccac
    14881 ggacgccgag tacttcgcct accagctcaa gtacgactcg gtccagggcc gcatgaaggc
    14941 ggacatctcc gccaagaaga gcgacgcatc cctcgccgag gccgacgtcc tggtcgtcaa
    15001 cggccacgag gtcaagtgcg tcatggccga gaaggaagtg aagaacctcc cct
//
LOCUS       DAOOSJ010000029        14679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2768_length_14679_cov_5.429841, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000029 DAOOSJ010000000
VERSION     DAOOSJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_14679_cov_5.429841"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLG14_02715"
     CDS             <1..690
                     /locus_tag="PLG14_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48074.1"
                     /translation="TLLLFSAGLLSSCNAAVDAGIRADGGARLGIAAEVPPVLGAKLR
                     KLSGAAPEAPLFDEAAVRSSAKGRPGLSVVSLAPTGNDGLKAEFAASSLKALAAEPEL
                     ASSGLLTYSEGPGWAELRVRLARGGGREALDLFPFVDPYLVEALSPPALEEDPVTADE
                     YRQMLKSVLGDKALAALETTSLGLRIQAPAAVLSSGGGSLSGTELRVALPVMELLVLE
                     KPVEFWLRWKR"
     gene            complement(695..1459)
                     /locus_tag="PLG14_02720"
     CDS             complement(695..1459)
                     /locus_tag="PLG14_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOX48075.1"
                     /translation="MRLLADLHNHSCLSPCASLELSPSLLARLARERGLGLLALTDHN
                     AALNCPAFGAACRREGLAPLFGLEACSLEEVHVLCLFGTAEAALAFGGRLKGLLLELP
                     YDPEKLGDEVVVDEEENVLELPDYYLGAALGLGFDELCSEAAAAGALVVPAHVDRPMF
                     SVGSQLGFLPPGPYAAVESMREPPPELRLGYPAVSGSDAHYPEHVGRRPFGLELPWEL
                     LKAASAGEGGAGEALLAALARALESGPLLPSWARGA"
     gene            complement(1625..1939)
                     /locus_tag="PLG14_02725"
                     /pseudo
     CDS             complement(1625..1939)
                     /locus_tag="PLG14_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1953..>2873)
                     /locus_tag="PLG14_02730"
     CDS             complement(1953..>2873)
                     /locus_tag="PLG14_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOX48076.1"
                     /translation="VPISPDAGPQVVLDLIYRLKVRDVMSTPVITASAGETMRTIQYR
                     MRDNGITGVPVVDGHGRLAGLVSIQDVIEALDKGRIGAKAEELMTKTVIVLEDDMPLA
                     FATSYFNRYKYGRFPVLDREGSLVGMVSASDIIRALLVAMNDEVQRLEERLSERLAAE
                     AESRPPDGKTRLSFPVVRFDFENAGKASAEIKKALKERGIDPATVRRAAVASYELELN
                     EVIHSEGGTLSCSIGEGRIEIVAEDRGPGIPDLDAAMREGYSTANEWIRSLGFGAGMG
                     LPNARRVSDEFSLDSGPGRGTRVLAAIKYQ"
     assembly_gap    2874..2973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2975..4276
                     /locus_tag="PLG14_02735"
     CDS             2975..4276
                     /locus_tag="PLG14_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX48077.1"
                     /translation="MNTFAPRAGRTALLASLLLALLCPPAARAQASPQAAPPSPAAAQ
                     PATSAAPAAAPILFAFSYAKGDKFRVLSEVDEEVYIDRRLSHRAEILNRIAFEVADAA
                     HDGSSGRLEGSFVTSERQVGASAFVVSESYDSAFVRDSRGRYTIGPEYFMPVVRNVPV
                     FPDRALLPGDTWTAPGEERHDLRRVFGIPDPYAIPFEARYRYEGPVLREGRKLELVSC
                     SYTVFHQPPPPRAYDFIYPTEIAGYSEQKIYWDPALGQPAAYEERFKLVFDWSDGRRI
                     EYRGTAKAQVLEAELMDRAALAAEVAKAVEGLENVSVAQTAEGVSISIEDIRFQPDSA
                     RLLPAELDKIARIAEILKRYPGRDILVAGHTALAGTAEGRKLLSEERARAVAERLIAS
                     GARVAERIQVAGYGAERPVADNATEAGKARNRRVEIVILEN"
     gene            complement(4404..6014)
                     /locus_tag="PLG14_02740"
     CDS             complement(4404..6014)
                     /locus_tag="PLG14_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOX48078.1"
                     /translation="MDFGREAAEPGSGLRGFQRKAFGLFAEMPHRGSGTHYEARAARM
                     LSRLAAESLEAQVELEPFAVDLSSGAWNVALHGTLLCLVLALDWLAGLGLAAAYGPGA
                     RAAGLGFLGSVPLPFAAVALGTTLLLLASRISAGKLGWGVFSFFVPNADSADIVVSDL
                     PRLSDGDSLRLAPAERWKRRFEASGKKRLLILAAHYDSARCLPPSKGKGAPPDCLLAA
                     LKNGIGLIPTAAYLLLALSFGLSVLLAFLDPSGALSRGAPGWIAAVLVLGSFGVAAVE
                     AFIAGKSVNLPFQQGYNDDLSGVVAALGAFRGLAPRDAPRAEPAAAASAGTALADTAL
                     MAVLTGSEENGLRGASEFARRVLAPAVGVFGKERVLLVNLDSVSGGELVAAAGERDFT
                     GATKSGDPDFARKAVDFLRGPRLQAARAAESLAPKELEASLEPAGSGAFAYRLRLEPE
                     PLPACTDMTGFCSVGGLNRRLRAFTVVSREAGSGPLPQPRDYHQPSDTLETLFLESEP
                     ANFGNVLALSLALADLAAEADKGSFDGE"
     gene            complement(6031..7317)
                     /locus_tag="PLG14_02745"
     CDS             complement(6031..7317)
                     /locus_tag="PLG14_02745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX48079.1"
                     /translation="MHELLLADRVKGEREIAVFRLVLFGATALMVVLMALTGSGFNLS
                     DWANLGAVGLAVVYTVALLLSIQRFGYHRAYGYASATIDVLLISASTYLCRYAASSSV
                     ASIVSTSSFAFYFPIILFSVRRHDPENTLYSGILSALAYGAMIVIMQVEGSFGVEMTG
                     SGGLVIRNDLFNEALKAIVLVVMGFVGFAAARRFDRLFEDALAALREKEQIRDMFGRY
                     VSDELVEKILAREISVEGEKRRATIMFIDIRNFTPLAEAVDPRVLITILNNFFSVCIS
                     TITKHGGFIDKFIGDAIMVVFGAPEPNERHSDAAVACALELSEALGTMNAWVRGLGVQ
                     WEFGYGIGINTGEVIVGNIGTEQRMEYTALGDAVNVASRLERLTRQLERPILVGEDCA
                     SACAAEPGCRWSFEGPYNARLKGKSEAVKVYSVRAR"
     gene            complement(7480..7920)
                     /locus_tag="PLG14_02750"
     CDS             complement(7480..7920)
                     /locus_tag="PLG14_02750"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HOX48080.1"
                     /translation="MTMPNALPAAFVAAALLALAALLLLAVLLGLALGRRGGRLEAER
                     GLEGRMAAERGDAVKRSRAVLGGQAAEQLAPWLPGFPYDPTECRFVGKPVDFVVFKGA
                     ASGEVEELVLVEVKSGGSSLSKVERSLREAVESGRVRWAEYRVP"
     gene            8017..>9209
                     /locus_tag="PLG14_02755"
     CDS             8017..>9209
                     /locus_tag="PLG14_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOX48081.1"
                     /translation="MPFLIAHPRDLAARGVEPGGLDAVFVTGDAYVDHPSFGAALLGR
                     LLEAEGFKVGILARPDPDDVEAFRLLGRPRLAFLVAAGALDSMVSSYTANNKPRSEDD
                     YAPGGRPELCLRADGTVGPGKAGRRNARPDRTTIAYAAKCREAYKGVPVVVGGVEASL
                     RRLAHYDYWSDKVRRSILLDSKADLLVHGMGERALLEIMRRLKAAADSGGDLAGAAAA
                     IRGLRGTAWRAGKRPELEAGSFEEVPPYARAQADKAAYAEHFRAQYRNSEAASARALL
                     EETDGRFVVAEPPAFPLAGPELDRIYELPYERAAHPMYEALGGVPALEEVKFSLVSSR
                     GCFGACSFCAIALHQGRLVTARSRESLLREAKVLTGLPGFKGYIHDVGGPTANFRGPA
                     CAKMAR"
     assembly_gap    9210..9309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9310..10005
                     /locus_tag="PLG14_02760"
     CDS             <9310..10005
                     /locus_tag="PLG14_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HOX48082.1"
                     /translation="AGAQAPVGACVDRRCLAPGPCPNLEVEEGGYLEILRALRALPGV
                     KKVFIRSGIRFDYLMLDPDESFFRELVEFHVSGQLKVAPEHVSDKVLALMGKPGRASY
                     DAFARRFAELNRELGLKQYLVPYFISAHPGAGLEEAVELAESLRDSGFVPDQVQDFYP
                     TPGSLSTAMYWTGLDPLSGNPVHVARGARERAMQRALLQYKKPENRELVREALLLAGR
                     RDLVGPGPKCLVR"
     gene            complement(10087..>11620)
                     /gene="pyk"
                     /locus_tag="PLG14_02765"
     CDS             complement(10087..>11620)
                     /gene="pyk"
                     /locus_tag="PLG14_02765"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOX48083.1"
                     /translation="YSLLFTITRFALNYAARIEARYRMNMLDKRTKIVATLGPASESP
                     ETVRALLEAGVNVFRLNFSHKSPEEAAVAIAMVRAARAELRVPAAIMADIKGPAVRLY
                     GYGKPLAVESGSVLAIESRPAEGIEALQAPEGVAYTNLPEIDRLCEAGQRVLLMDGYF
                     AGSVVARSPGRVEVKLGNPGSLRPKAHLTIPNVDYPIPFLSEKDIRDISFAVEKEVEY
                     IALSFVRCATDIEEVRRLVARSRTESGARPNALLVAKIESARGLASVDEIVEAADGIM
                     VARGDMGVEISVESVPLAQKRIIRTGYLAAKPVITATQMLESMIESPIPTRAEASDVA
                     NACFDSTSAVMLSGETAMGRFPVKVVETMGSIIRAVEAEFDYEGFHGSVPPNVRSGDL
                     PAIMSYNAVSTAYQCGASALAVLTETGHAARLLSRLRPRMPILAFTRDEAVYHQLALN
                     WGVRPILLASPYGRLEEAVAMILEETAKAGIASKGDRLVIVAGLPLARRGTTNMIRVE
                     TL"
     assembly_gap    11621..11720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11721..12005
                     /locus_tag="PLG14_02770"
     CDS             <11721..12005
                     /locus_tag="PLG14_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOX48084.1"
                     /translation="TLQDLGELFRLFADPTRLGILSALSGGELCVCDLTLVLGMSQSA
                     VSHQLALLRTARLVKNRREGKTVFYSLDDEHVREILLVGLSHLSERKEAR"
     gene            12002..14233
                     /locus_tag="PLG14_02775"
     CDS             12002..14233
                     /locus_tag="PLG14_02775"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX48085.1"
                     /translation="MTSYRLKGLDCPTCAGALEEGLRKLEGVSSASVDFGSLSLKIEA
                     EDLAAVEAAIPRLEPGVTAERLGAGGRGAGRPGGGQAAGGASVRASGGCGGEPGSCPS
                     CSSCADSGAPGEAEGGFSLRRELALFGASALVAAAAFALEKTGAAAGLGRAGRLAPPL
                     LYLAAYLLAGWNVVAGAFRNILRGRVFDELFLMTIATFGAFAIGQFEEAVGVMVFYKI
                     GEMLQESAAEKSRASVRGLLSLRPDRARLRRDGDWALLPAGEAEIGEEYLVLPGERIP
                     LDGTVVGGEAFVDTQALTGEPVPRRAAPGTEILAGTLALDGALTARAARLAGESSAAR
                     IADLVENASHAKARTERFVTRFARVYTPIVVVLAALVAFLPPLLLGQSLAAWAYRALV
                     MLVISCPCALVVSVPLGYFGGLGGAARRGILVKGATVLDALAEAKTVVFDKTGTLTKG
                     VFKVLAVEPAAGRSEAELLELAAAAESRSRHPIAASIREAARERGLSDGTEDEASLVR
                     EFPGMGVVAELGGRRVVAGNARALELEGVGLGAYEAEGTTVHVAVDGAYAGRLLIGDE
                     AKPDAARAVAELARLGVGRSAMLTGDEEKAALPLAALLGVGEVHAGLLPGGKLAILER
                     IIAETNAAGGKTIFVGDGVNDAPVLARADVGVAMGAGADAAVESADLVLMTDEPSRVA
                     EAVARARRTRRIVRQNIVFSLAVKAGFLALGAMGVAVMWEAVIADVGVALLATLNATR
                     AMR"
     gene            complement(14279..14665)
                     /locus_tag="PLG14_02780"
     CDS             complement(14279..14665)
                     /locus_tag="PLG14_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48086.1"
                     /translation="MAAPRVLAADDDECNLDILRRMLLRLGAEVQTARDGAEAARLAL
                     GEAAAGRPFDLVLLDLSMPVLGGAEAARRIKAGLPGAVVLALSGADEEEAALEPGFDG
                     FAQKPITTDLVRGLLGRWCGGGGAAS"
BASE COUNT         1963 a         5231 c         5249 g         1936 t
ORIGIN      
        1 accctcctcc tcttctcggc gggcctgctt tcctcctgca acgccgccgt ggacgcgggc
       61 atccgcgccg acggcggcgc ccgcctcggg atagccgccg aggtgccgcc ggtcctgggc
      121 gccaagctca ggaagctctc cggcgcggcc ccggaggcgc ccctcttcga cgaggccgcg
      181 gtcaggagct cggccaaggg ccggccgggc ctgtccgtcg tcagtctggc gccgacgggg
      241 aatgacgggc tcaaggccga gttcgccgcc tcgagcctca aggccctcgc cgccgagccc
      301 gagctcgcct cctcgggcct cctcacttat tctgagggcc ccggctgggc cgagctccgc
      361 gtgcggctcg cccgcggcgg cggccgcgag gccctcgacc tcttcccctt cgtcgacccc
      421 tacctcgtgg aggccctctc cccgcccgcc ctggaggagg atcccgtcac cgccgacgag
      481 taccggcaga tgctcaagag cgtcctgggg gacaaggccc tggccgccct ggagacgacc
      541 agcctcggcc tccggatcca ggcgccggcg gccgtcctct cctcgggcgg cggctcgctg
      601 tcggggacgg agctccgcgt ggccctgccc gtgatggagc ttctcgtcct cgagaagccc
      661 gtcgagttct ggctgcgctg gaaacgctga tcccctaggc gccgcgcgcc caggagggga
      721 ggagggggcc gctctccaag gcccgggcca gggcggcgag gagggcctcg cccgccccgc
      781 cctcccccgc cgaggccgcc ttgaggagct cccaagggag ctcgaggccg aagggccggc
      841 ggcccacgtg ctccgggtag tgggcgtcgg agcccgagac ggcgggatag cccagcctga
      901 gctccggggg cggctcgcgc atcgactcga cggcggcgta gggcccgggc ggcaggaagc
      961 cgagctggct ccccacggag aacatgggcc ggtccacgtg ggcggggacc acgagggctc
     1021 ccgccgcggc ggcctcggag caaagctcgt cgaagcccag gcccagggcc gcgcccaggt
     1081 agtagtccgg cagctcgagg acgttctcct cctcgtccac gacgacctcg tcgccgagct
     1141 tctccgggtc gtagggcagt tcgaggagga gacccttgag ccgcccgccg aaggccaggg
     1201 cggcctcggc cgttccgaag aggcagagga cgtggacctc ctccagggag caggcctcga
     1261 ggccgaagag cggcgcgagc ccctcgcggc ggcaggccgc gccgaaggcg gggcagttca
     1321 gggcggcgtt gtggtccgtg agggcgagga ggcccaggcc ccgctccctg gccagccgcg
     1381 cgaggaggct cggcgagagc tccagggagg cgcagggcga gaggcaggag tggttgtgga
     1441 ggtcggcgag gaggcgcatg gcgctgggag ccccttagtc cgcgggcttc gcctgaaggc
     1501 atagcctcaa ggctcggccc gggccggact aggctttgcc ggccgcgcca ggcccggcgc
     1561 caggcgagcc cgcggcgcgc ggctggcccg cgccgcccgc ctgccccgcc ccgcccagct
     1621 cgggccagag gaggcccgag acctcgaact ggctcctgtc ggtgaggaag acggccacgc
     1681 cctcggcctt cgcggcctcc agcatgtcct cggggacggg gcggccgttg cagaccacga
     1741 tggcggccag gttgaccagg ctcgcgacgg cgaccgtgtt cttgtgcgcc tggatcgtca
     1801 cgaggacccc ctcggacttg gcgttggcca tgacgtcgga gaggaggtcg gaggtgtagc
     1861 ccgccgcaag ggggcggtcg gcgtactcgg cctgcaggga cttgaagccg aagcctcgct
     1921 cgaggtcgct tatcttcatg gaagggctcc tttcactggt acttgattgc ggcgaggacc
     1981 ctcgtgcccc tgccggggcc cgaatcgagg gagaactcgt cggagacgcg cctcgcgttc
     2041 ggcaggccca tgccggcgcc gaagcccagg gagcggatcc attcgttggc cgtcgagtag
     2101 ccctcgcgca tcgccgcgtc caggtcgggt atgcccgggc cccggtcctc ggcgacgatc
     2161 tcgatccgcc cctcgccgat ggagcaggag agggtcccgc cctcggagtg gatcacctcg
     2221 ttgagctcga gctcgtagct cgcgacggcc gcgcggcgga cggtggcggg gtcgatgccc
     2281 cgctccttca gggccttctt gatctcggcg ctggccttgc ccgcgttctc gaagtcgaag
     2341 cggaccacgg ggaaggagag cctcgtcttg ccgtcggggg gcctcgactc ggcctccgcg
     2401 gccaggcgct cggagagccg ctcctccagg cgctggacct cgtcgttcat cgcgacgagg
     2461 agggcgcgga tgatgtcgga ggcggagacc atgccgacca ggctcccctc ccggtccagg
     2521 acggggaagc ggccgtactt gtagcggttg aagtaggagg tggcgaaggc gaggggcatg
     2581 tcgtcctcca ggacgatgac cgtcttcgtc atgagctcct cggccttggc ccctatccgg
     2641 cccttgtcca gggcctcgat cacgtcctgg atggagacga ggcccgccag gcggccgtgg
     2701 ccgtcgacga cgggcacgcc cgttatgccg ttgtcgcgca tcctgtactg gatggtccgc
     2761 atggtctcgc cggcggaggc cgtgatgacg ggcgtgctca tcacgtcgcg gaccttgagg
     2821 cggtagatga ggtcgaggac gacctggggg cccgcgtcgg ggctgatggg gacnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatgaac accttcgccc cgcgggccgg
     3001 ccggacggcc ctcctcgcca gcctgctcct cgccctcctt tgccccccgg ccgcccgggc
     3061 ccaggccagc ccccaggcgg cccccccctc gcccgccgcg gcccagccgg cgacctccgc
     3121 cgcgcccgcc gcggccccca tcctcttcgc cttctcctac gcgaagggcg acaagttccg
     3181 cgtcctctcc gaggtggacg aggaggtcta catcgaccgc cgtctctccc accgggcgga
     3241 gatcctgaac cgcatcgcct tcgaggtggc ggacgcggcg cacgacggga gctcgggcag
     3301 gctcgagggg agcttcgtca cctcggagcg gcaggtgggc gcgagcgcct tcgtcgtctc
     3361 ggagagctac gactcggcct tcgtccgcga ctcgaggggc cgctacacga tcgggcccga
     3421 atacttcatg cccgtcgtca ggaacgtccc cgtcttcccc gacagggccc tcctgccggg
     3481 cgacacctgg acggcccccg gcgaggagcg ccacgacctc cgccgcgtct tcggcatccc
     3541 cgacccctac gccatcccct tcgaggcgcg ctaccgctac gagggccccg tcctccgcga
     3601 gggcaggaag ctcgagctcg tctcctgctc ctacacggtc ttccaccagc cgccgccgcc
     3661 ccgcgcctac gacttcatct atcccacgga gatagccggc tactccgagc agaagatcta
     3721 ctgggacccg gccctgggcc agcccgccgc ctacgaggag cgcttcaagc tcgtcttcga
     3781 ctggtcggac gggcgcagga tcgagtaccg cgggacggcg aaggcccagg tcctggaggc
     3841 ggagctcatg gaccgggcgg ccctggccgc cgaggtggcg aaggccgtcg agggcctcga
     3901 gaacgtgagc gtcgcccaga cggcggaggg cgtctccatc agcatcgagg acatccgctt
     3961 ccagcccgac tcggcgaggc ttctccccgc cgagctcgac aagatcgccc gcatcgcgga
     4021 gatcctcaag cgctaccccg gccgcgacat cctggtcgcc gggcacacgg ccctggcggg
     4081 cacggccgag ggcaggaagc tcctctccga ggagcgggcg cgcgccgtcg cggagcgcct
     4141 catcgcctct ggcgcccgcg tggccgagcg gatccaggtc gcgggctacg gggccgagcg
     4201 cccggtggcc gacaacgcga ccgaggccgg caaggcccgg aaccgccgcg tggagatcgt
     4261 catcctggag aactaggggc gccgggcggc gctgtgcgac ggccgcgccg gcccgcccgt
     4321 cgcgacgggg cgtggagccg cacggggtcg cggccgcgcc atgcgacggc cgcgtcaggc
     4381 cgcggcggcg ggcccgcgcc ccgctactct ccgtcgaagc tccccttgtc cgcctcggcc
     4441 gcgaggtcgg ccagggccag ggagagggcg aggacgttgc cgaagttcgc gggctccgac
     4501 tcgaggaaga gggtctctaa ggtgtcgctc ggctggtggt agtcgcgggg ctggggcagg
     4561 gggcccgagc ccgcctcgcg ggagacgacc gtgaaggcgc ggagcctcct gttgagcccc
     4621 ccgacggagc agaagcccgt catgtccgtg caggccggca ggggctcggg ctccaggcgg
     4681 aggcgatagg cgaaggcgcc ggaaccggcg ggctcgaggg aggcctcgag ctccttcggg
     4741 gccaggctct ccgccgccct ggccgcctga aggcgggggc cgcggaggaa gtcgaccgcc
     4801 ttccgggcga agtccgggtc gccgctcttg gtcgcacccg tgaagtcgcg ctcgcccgcg
     4861 gcggcgacga gctcgccgcc cgagaccgag tccaggttga cgagaagcac gcgctccttc
     4921 ccgaagaccc cgaccgcggg cgcgaggacc ctgcgcgcga actccgaggc gccgcggagt
     4981 ccgttctcct cagagcccgt gaggacggcc atcagcgccg tgtcggcgag ggccgtgccg
     5041 gcggaggccg cggcggcggg ctcggcgcgg ggggcgtccc tgggcgcgag gccccggaag
     5101 gcgccgaggg cggcgacgac gccggagagg tcgtcgttgt agccctgctg gaagggcagg
     5161 ttgaccgatt tcccggctat gaaggcctcc actgcggcga ccccgaagga gccgaggacg
     5221 aggacggcgg cgatccagcc cggcgcgccg cgggaaaggg cccccgaggg gtccaggaag
     5281 gcgaggagga cggaaaggcc gaaggagagg gcgaggagga ggtaggccgc ggtgggaatg
     5341 aggccgatgc cgttcttgag cgcggcgagg aggcaatccg gcggcgcgcc cttgcccttg
     5401 gagggaggca ggcagcgggc cgagtcgtag tgcgccgcga ggatgaggag ccgcttcttg
     5461 cccgaggcct cgaagcggcg cttccagcgc tcggccgggg ccagcctgag ggagtccccg
     5521 tcggagaggc ggggcaggtc ggagacgacg atgtccgccg agtcggcgtt cggcacgaag
     5581 aaggagaaga ctccccatcc tagcttcccc gcggagatcc gcgaggcgag gaggaggagg
     5641 gtcgttccga gggcgacggc cgcgaagggg aggggcacgg agcccaggaa gccgaggccg
     5701 gccgccctgg cgccggggcc gtaggccgcg gccaggccta ggccggcgag ccagtccagg
     5761 gccaggacga ggcagaggag ggtcccgtgg agggcgacgt tccaggcccc gctcgagagg
     5821 tcgacggcga agggctcgag ctcgacctgc gcctccaggg actcggcggc caggcgggag
     5881 agcatcctgg ccgcccgcgc ctcgtagtgc gtgcccgagc cgcggtgcgg catctcggcg
     5941 aagagcccga aggccttcct ctggaagccc ctgagcccgg agcccggctc cgcggcctcg
     6001 cgcccaaagt ccatgccgac ctccttgccg ctacctggcc ctgaccgaat agaccttcac
     6061 ggcctcgctc ttgcccttga gccgcgcgtt gtaggggccc tcgaagctcc agcggcagcc
     6121 gggctccgcc gcgcaggccg aggcgcagtc ctcgcccacc aggatgggcc gctcgagctg
     6181 gcgggtgagc cgctcaaggc ggctcgcgac gttgaccgcg tcgcccaggg ccgtgtactc
     6241 catgcgctgc tcggtgccga tgttgccgac gatcacctcg cccgtgttga tgccgatgcc
     6301 gtagccgaac tcccactgga cgcccaggcc gcggacccag gcgttcatcg tgcccagggc
     6361 ctcggagagc tccagggcgc aggcgaccgc cgcgtcggag tgccgctcgt tcggctccgg
     6421 cgcgccgaag acgaccatga tcgcgtcgcc gatgaacttg tcgatgaagc cgccgtgctt
     6481 cgtgatggtg gagatgcaga ccgagaagaa gttgttgagg atggtgatga ggacccgcgg
     6541 gtcgacggcc tccgcgagcg gcgtgaagtt cctgatgtcg atgaacatga tcgtcgcccg
     6601 gcgcttctct ccctccacgg agatctcgcg cgccaggatc ttctcgacca gctcgtccga
     6661 gacgtagcgg ccgaacatgt ccctgatctg ctccttctcc cggagcgcgg ccagggcgtc
     6721 ctcgaagagc ctgtcgaagc gccgcgccgc cgcgaagccc acgaagccca tgacgacgag
     6781 gacgatggcc ttgagcgcct cgttgaagag gtcgttccgg atcacgaggc cgcccgagcc
     6841 cgtcatctcg acgccgaagg agccctcgac ctgcatgatg acgatcatcg cgccgtaggc
     6901 cagggcggag aggatgcccg agtagagggt gttctcgggg tcgtggcgcc ggacggagaa
     6961 gaggatgatc gggaagtaga aggcgaagga gctcgtcgac acgatgctcg cgaccgagga
     7021 ggaggcggcg tagcggcaga ggtaggtgct cgccgagatg aggaggacgt cgatggtcgc
     7081 ggaggcgtag ccgtaggccc ggtggtagcc gaagcgctgg atggagagga gaagggcgac
     7141 ggtgtagacg acggcgaggc cgaccgcgcc gaggttggcc cagtccgaga ggttgaagcc
     7201 cgagccggtc agggccatga ggaccaccat cagggcggtg gcgccgaaga ggacgaggcg
     7261 gaagacggcg atctcgcgtt cgcccttgac tcggtctgcc aggaggagct cgtgcatcga
     7321 tgttcctttc ttgtggatcg cttccaggca agtatagccc gtatcggaaa agccgcaacc
     7381 ggcggcgggc cgggcgcagc cgggccgccg ccccggagcc ggcctcggcg cggggcccaa
     7441 aggccggagg cccagcggcg gcgaacgggg gacccggccc tagggaaccc ggtactcggc
     7501 ccagcgcacc ctgcctgact ccacggcctc gcggaggctc cgctcgacct tgctcaggga
     7561 ggagccgccc gacttcacct cgacgaggac gagctcctcg acctctcccg aggctgcgcc
     7621 cttgaatacg acgaagtcga cgggcttccc gacgaagcgg cattccgtgg ggtcgtaggg
     7681 gaagccaggg agccatggcg cgagctgctc ggcggcctgg ccgcccagga cggcccgcga
     7741 gcgcttcacg gcgtcgccgc gctcggcggc catccgcccc tccaggcccc gctcggcctc
     7801 caggcggccg ccccgccgtc cgagggcgag gccgaggagg acggcgagga ggaggagggc
     7861 ggcgagggcg aggagggcgg ccgcgacgaa ggcggcgggg agggcgttcg gcatcgtcat
     7921 ggcgtgggcg attctagcct cggccggagc cggcgtcgag ccgggcgcgg ccccgccccc
     7981 ctgacccggg cacgccgccc cgcgtatact ctctccatgc ccttcctcat cgcccatccc
     8041 cgcgacctcg ccgcccgcgg cgtcgagccc ggcggcctcg acgccgtctt cgtcacgggc
     8101 gacgcctacg tcgaccatcc ctccttcggc gcggccctcc tcggccgcct cctcgaggcg
     8161 gagggcttca aggtcgggat cctcgcgagg cccgaccccg acgatgtgga ggccttccgc
     8221 ctcctcgggc ggccgcgcct cgccttcctc gtcgccgcgg gcgccctcga ttccatggtc
     8281 tcctcctaca ccgcgaacaa caagccgcgc tcggaggacg actacgcgcc gggcggccgc
     8341 ccggagctct gcctgcgcgc cgacgggacg gtcggtcccg gaaaggccgg gcgccgcaac
     8401 gcgcggcccg accgcacgac catcgcctac gccgcgaagt gccgcgaggc ctacaagggc
     8461 gtccccgtcg tcgtgggcgg cgtcgaggcc tcgctcaggc gccttgccca ctacgactac
     8521 tggtccgaca aggtgaggcg ctccatcctc ctcgactcga aggccgacct cctcgtccac
     8581 gggatgggcg agcgggccct cctcgagatc atgcggaggc tgaaggccgc ggccgactcg
     8641 ggcggggacc tcgccggggc cgccgccgcg atccgcggcc tgcgggggac ggcctggcgg
     8701 gcggggaagc gccccgagct ggaggccggc tccttcgagg aggtcccgcc ctacgcccgg
     8761 gcccaggcgg acaaggccgc ctacgccgag cacttccgcg cccagtaccg caactccgag
     8821 gccgcctcgg cccgcgccct cctcgaggag accgacggcc gcttcgtcgt cgcggagccg
     8881 cccgccttcc ccctcgcggg ccccgagctc gaccggatct acgagctacc ctacgagcgg
     8941 gccgcccacc cgatgtacga ggccttgggg ggcgttcccg ccctggagga ggtgaagttc
     9001 tccctggtgt cgagccgggg ctgcttcggg gcctgctcct tctgcgctat cgcgctgcac
     9061 caggggaggc tcgtcacggc gagaagccgc gagtccctcc tccgcgaagc caaggtcctc
     9121 acgggactgc ccggcttcaa gggctacatc cacgacgtgg gcggccccac cgcgaacttc
     9181 cgcggccccg cctgcgccaa gatggcccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnng cgggcgccca ggcgccggtc ggcgcctgcg tcgacaggcg ctgcctggcc
     9361 ccaggcccct gcccgaacct cgaagtcgag gagggcggct acctggagat cctccgggcc
     9421 ctgcgggccc tgccgggcgt caagaaggtc ttcatccgct cggggatccg cttcgactac
     9481 ctcatgctcg accccgacga gtccttcttc cgcgagctcg tcgagttcca cgtctcgggc
     9541 cagctcaagg tcgcgcccga gcacgtctcc gacaaggtcc tcgccctgat gggaaagccg
     9601 ggccgggcct cctacgacgc cttcgcgcgg cgcttcgccg agctcaacag ggagctcggc
     9661 ctcaagcagt acctcgtgcc ctacttcatc tcggcgcacc cgggcgcggg cctcgaggag
     9721 gccgtcgagc tcgcggagag cctcagggac tcgggcttcg tccccgacca ggtccaggac
     9781 ttctacccga cgcccggcag cctctcgacc gcgatgtact ggaccgggct cgacccgctg
     9841 tcggggaacc ccgtccacgt cgcgcgcggc gcccgcgagc gcgccatgca gcgggccctc
     9901 ctccagtaca agaagcccga gaaccgcgag ctcgtcaggg aggccctgct cctcgcgggc
     9961 cgccgcgacc tcgtcggccc cggccccaag tgcctggtcc gctgaaggcg cggcgcggcg
    10021 cccgcgggcc gggcggcgcc ggcgcggggc ccgggccgcg tccggcgccc cccgcgccgc
    10081 ccccgcctag agcgtctcca cgcggatcat gttcgtcgtg ccgcgccgcg cgaggggaag
    10141 gcccgcgacg atgaccaggc gatcgccctt cgacgcgatg cccgccttcg ccgtctcctc
    10201 caggatcatc gcgacggcct cctccagccg gccgtagggc gaggcgagga ggatggggcg
    10261 gacgccccag ttaagggcga gctggtggta gacggcctcg tcgcgggtga aggccaggat
    10321 gggcatccgc ggcctgaggc gcgagaggag gcgggccgcg tggccggtct ccgtgaggac
    10381 ggccagggcc gaggcgccgc actggtaggc cgtggacacc gcgttgtagg acatgatcgc
    10441 gggcaggtcg ccggagcgca cgttgggcgg cacggagccg tggaagccct cgtagtcgaa
    10501 ctccgcctcc accgcgcgga tgatggagcc catcgtctcc acgaccttca cggggaagcg
    10561 gcccatcgcg gtctcgccgg agagcatcac ggcgctcgtg gagtcgaagc aggcgttcgc
    10621 gacgtcgctc gcctccgccc tcgtcgggat ggggctctcg atcatcgact cgagcatctg
    10681 cgtcgcggtg atgaccggct tggcggcgag gtagcccgtc ctgatgatgc gcttctgcgc
    10741 cagggggacg ctctccacgg agatctccac gcccatgtcg ccgcgcgcta ccatgatgcc
    10801 gtcggccgcc tcgacgatct cgtccacgga ggcgaggccc cgcgcgctct cgatcttcgc
    10861 gaccaggagg gcgttcggcc tcgcgccgga ctcggtgcgg gagcgggcga ccaggcggcg
    10921 gacctcctcg atgtcggtgg cgcagcggac gaaggagagg gcgatgtact cgacctcctt
    10981 ctcgacggcg aaggagatgt cgcggatgtc cttctcggag aggaagggga tcgggtagtc
    11041 gacgttgggg atcgtgaggt gggccttggg gcggagggag cccggattgc cgagcttgac
    11101 ctccacgcgg ccgggggagc gcgcgacgac ggagccggcg aagtagccgt ccatgaggag
    11161 gacgcgctgg ccggcctcgc agagtcggtc gatctcgggg aggttcgtgt aggcgacgcc
    11221 ctccggcgcc tggagggcct cgatgccctc cgccggccgg ctctcgatgg cgaggaccga
    11281 gcccgactcg acggcgaggg gcttgccgta gccgtagagc ctgacggcgg ggcccttgat
    11341 gtcggccatg atcgccgcgg gcaccctgag ttcggcgcgg gccgccctga ccatcgcgat
    11401 ggcgacggcg gcctcctccg ggctcttgtg ggagaagttg aggcggaaga cgttgacccc
    11461 cgcctccagg agggcgcgga cagtctccgg gctctcggag gccgggccca gggtcgccac
    11521 tatcttcgtg cgcttgtcga gcatgttcat cctgtacctc gcttctattc ttgccgcata
    11581 gtttagcgca aaacgcgtta tcgtgaaaag aagcgaatag nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn accctccagg acctgggcga gctcttccgc ctcttcgccg
    11761 acccgacgcg gctcggcatc ctctccgccc tctcgggcgg ggagctctgc gtctgcgacc
    11821 tcaccctcgt cctcgggatg agccagtccg cggtgagcca ccagctcgcc ctcttgagga
    11881 cggcccgcct cgtcaagaac cgccgcgagg gcaagacggt cttctattcc ctcgacgacg
    11941 agcacgtgag ggagatcctc ctcgtgggct taagccacct ctcggagaga aaggaagcgc
    12001 gatgaccagc tatcgcctca aggggctcga ctgccccacc tgcgcgggag ccctcgagga
    12061 gggcctccgg aagctcgagg gagtctcctc ggcaagcgtc gacttcggaa gcctctcgct
    12121 caagatcgag gccgaggatc tcgccgccgt cgaggcggcc atcccccgcc tcgagcccgg
    12181 cgtcacggcc gagcgcctag gcgcaggcgg ccgaggagcg ggccggcccg gcggcggcca
    12241 ggcagccggc ggcgcgagcg tccgcgcgtc cggcggctgc ggcggggagc cgggatcctg
    12301 tccttcctgc tcctcctgcg cggactcagg agcgccagga gaggccgagg gcggcttcag
    12361 cctgaggcgg gagctcgccc tcttcggcgc ctcggccctc gtcgccgcgg cggccttcgc
    12421 cctcgaaaag accggagccg cggccggcct cgggcgggcg ggccgcctcg cccctcccct
    12481 cctctacctc gcggcctacc tcctggcggg ctggaacgtc gtggcgggcg ccttccgcaa
    12541 catcctccgc ggccgggtct tcgacgagct cttcctcatg acgatagcca ccttcggcgc
    12601 cttcgccatc ggccagttcg aggaggccgt cggcgtgatg gtcttctaca agatcggcga
    12661 gatgctccag gagagcgccg cggagaagag cagggcctcg gtccgcggcc ttctctccct
    12721 gcgccccgac cgggccaggc tcaggcggga cggggactgg gccctccttc ccgccggcga
    12781 ggcggagatc ggcgaggaat acctcgtcct cccgggcgag cggattcccc tggacggcac
    12841 cgtcgtcggc ggcgaggcct tcgtcgacac ccaggccctg acgggcgagc ccgtcccgcg
    12901 ccgcgccgcg ccggggacgg agatcctcgc gggcaccctg gccctggacg gcgccctcac
    12961 cgcccgcgcg gcgcggctcg ccggggagtc ctccgccgcg cgcatcgccg acctcgtgga
    13021 gaacgcctcc cacgccaagg cgcggaccga gcgcttcgtc acgcgcttcg cccgggtcta
    13081 cacgcccatc gtcgtcgtcc tcgcggccct ggtcgccttc ctgccgcccc tcctcctcgg
    13141 gcagtccctc gccgcctggg cctaccgcgc cctcgtgatg ctcgtcatct cctgcccctg
    13201 cgccctcgtc gtctccgtgc ccctgggcta tttcggcgga ctgggcggcg cggcccgccg
    13261 cggcatcctc gtgaagggcg ccaccgtcct cgacgccctg gccgaggcca agaccgtcgt
    13321 cttcgacaag acgggcacgc tcacgaaagg cgtcttcaag gtcctggccg tggagcccgc
    13381 ggccggccgc tccgaggccg agctcctgga gctcgcggcc gccgccgagt cccgctcccg
    13441 ccacccgata gccgcctcga tccgcgaggc ggcccgggag cggggcctct cggacggcac
    13501 ggaggacgag gcctccctcg tccgggagtt ccccgggatg ggcgtcgtcg ccgagctcgg
    13561 cggccgccgc gtcgtcgccg gcaacgcccg cgccctcgag ctcgagggcg tcggcctggg
    13621 cgcctacgag gccgagggca cgacggtcca cgtcgccgtc gacggcgcct acgcgggccg
    13681 cctcctcatc ggcgacgagg ccaagcccga cgccgcgagg gccgtcgcgg agctcgcgcg
    13741 gctgggcgtg ggccggtccg cgatgctgac cggcgacgag gagaaggccg ccctgcccct
    13801 cgccgccctc ctcggcgtcg gggaggtcca cgcgggcctc ctccccgggg ggaagctcgc
    13861 gatcctggag cggatcatcg cggagacgaa cgcggcgggc ggcaagacca tcttcgtcgg
    13921 ggacggcgtc aacgacgccc ccgtcctcgc gagggccgac gtcggcgtcg cgatgggcgc
    13981 gggcgccgac gccgcggtgg agagcgccga tctggtcctc atgacggacg agccctcccg
    14041 cgtggcggag gccgtggcca gggcgcgccg cacgcggcgc atcgtccgcc agaacatcgt
    14101 cttctccctc gccgtcaagg cgggcttcct cgccctgggg gccatgggcg tcgcggtgat
    14161 gtgggaggcg gtgatcgccg acgtgggcgt ggccctcctc gcgaccctga acgcgacgcg
    14221 ggcgatgcgt taggcggcac atgccgccgc gtcgcgcgcc gccgcgtggc gctcgccgtc
    14281 aggaggcggc gcctccgccc ccgcaccagc gcccaaggag gccgcgcacc aggtccgtcg
    14341 tgatgggctt ctgcgcgaag ccgtcgaagc ccggctccag ggcggcttcc tcctcgtcgg
    14401 cgccggacag ggccaggacg acggcgccgg gcaaacccgc cttgatgcgc cgcgcggcct
    14461 cggccccgcc gagcaccggc atcgagaggt cgaggaggac gaggtcgaag ggcctgcccg
    14521 ccgcagcctc tcccagggcg agcctggccg cctcggcccc gtccctggcc gtctggacct
    14581 cggctccgag cctcaggagc atgcgcctca ggatgtccag gttgcactcg tcgtcgtcgg
    14641 cggcgaggac ccgcggggcg gccacctagg cctccggcg
//
LOCUS       DAOOSJ010000030        14306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2855_length_14306_cov_4.839380, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000030 DAOOSJ010000000
VERSION     DAOOSJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_14306_cov_4.839380"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..89
                     /locus_tag="PLG14_02785"
     tRNA            7..89
                     /locus_tag="PLG14_02785"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Tyr,seq:gta)
     gene            141..635
                     /locus_tag="PLG14_02790"
     CDS             141..635
                     /locus_tag="PLG14_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOX48087.1"
                     /translation="MGDVFYGEGLRFSCARCSSCCRGGPGYVFLSKEDLKRLLLRTSL
                     AFPAFFAEYLTTVDTGTGYALSLREKPNYDCIFWGESGCAVYEDRPVQCSTFPFWSSI
                     LASEEDWRDAGRDCPGIGRGELRSRAYIEERLHARRAAGTLLLTKDEAAHPERIDADT
                     LLGM"
     gene            610..1548
                     /locus_tag="PLG14_02795"
     CDS             610..1548
                     /locus_tag="PLG14_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX48088.1"
                     /translation="MRIRFWGCRGSLPSPLVPAKLRSKISSVIGRVGPADIASPEARE
                     RFLAGLPEYLFSTVGGNTPCVEIATEEPERLVFDAGTGIRELGNEIVAAKKPVSCHLF
                     FSHFHWDHIQGLPFFGPAYDPRNELFFHSPVEGFRSILEGQMRQPYFPITMDQMSARK
                     DFTVLDGKPLRLGGVEVSYRPMSHPGGCYCYRVEEKGRKAIYSTDTELSEADFRKSPE
                     NEAFFRGVELLVIDTQYTLGEAIEKYNWGHSSFSLAADFAATWGIERLVLFHHEPSYS
                     DRKIHGNLKNASWYLDHLESRGIEVILAREGLEVEV"
     gene            1552..1788
                     /locus_tag="PLG14_02800"
     CDS             1552..1788
                     /locus_tag="PLG14_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48089.1"
                     /translation="MEDPRAADADFRPALGLKASLALFLLLEGRSDGIAGSAPELDRL
                     EDELRDYLYGNLSIADMENPGKTYSALAGRKERA"
     gene            1785..2828
                     /gene="mltG"
                     /locus_tag="PLG14_02805"
     CDS             1785..2828
                     /gene="mltG"
                     /locus_tag="PLG14_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOX48090.1"
                     /translation="MKKSPRRLALVLSLAVGLLAGLLAAGFAAFALALRPPAGLAEEG
                     LLFTVRAGEGAGILAARLEAEGAIRSALAFRLLAKARGDEGRLKAGVYRIEPAMGGRE
                     VLGAIVSGRQALVRLTVPEGATLSRVALILEEGGVAAAAEFRAAARDPALLSELGIPG
                     PSLEGYLFPDTYFMPLGYGAAGAARAMAAAFRSRLASIPEAAALSPEELREKIILASI
                     VEREYRVADEAPVMASVFYNRLRIGMALQSCATVVYVITEKLGKPHPERLFDRDIAIP
                     DAYNTYLARGLPPGPIANPGMTAIRSVFYPASTKYLYFRLVDESAGRHHFSETLEEHN
                     DARSLFVKRAGGK"
     gene            2825..4633
                     /gene="dnaG"
                     /locus_tag="PLG14_02810"
     CDS             2825..4633
                     /gene="dnaG"
                     /locus_tag="PLG14_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOX48091.1"
                     /translation="MSLIPEATVRTILDRTDIVAVIGDYVRLEKRGGRWLGLCPFHAE
                     KTPSFNVDREKGFFYCFGCRKGGDVVTFLREHEKMGYREALEELARRAGVPIELEEAP
                     SEEERARKALLELYERLAAAFHYLLVEHPSGEAARAVLRRRAIPDELREEFRLGYAPR
                     DRRWLHGFLLKKGYSADFLSRSGLFAERHPDFPLFADRLIFPIAQARGEVIAFGGRLL
                     EGEGPKYINSPDTALFHKQENLFALDKALPAIKKEGAALVCEGYMDALSFHAAGLRFA
                     VAPLGTAFTERQAKLLKRWASQVVLAFDADPAGQRAAEKSVVLAAAAGLEPRVAVLPG
                     GKDASEILEKEGPESLQKLRDLTINGSEFLVRRARELFDIGSVEGKAKAAAFLNPYAE
                     ALDSEVKRDAFFDLASRELKADPRSLRSDYEDAKKGISTRRTAKGASPEGSGPMPASR
                     TPDLLFMAAVATNADLFGGVRSLLSLEELDDPRARDLYIALEECFRAEETGLDSILGR
                     ITDEPLRVFVFERAATGEFSENAERFVEDGLKALRLRSLERRRAKVLERMADFRETGS
                     EAWTETEGPSLNDLMYEKMYLDAELAKIKGERDERS"
     gene            4620..6344
                     /gene="rpoD"
                     /locus_tag="PLG14_02815"
     CDS             4620..6344
                     /gene="rpoD"
                     /locus_tag="PLG14_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOX48092.1"
                     /translation="MNDLELDPAIAKLLEYAKAKKSISFDELSDFLPEHVLNSEKIDG
                     ILALLESNNIQLEEEEVAVEDEAPVKQETEKKRLVYNDKESSVDDPIRLYLREIGKEN
                     LLTAEQEVELSKSMEEGENIIKGIIKRSGVLISEFHLIAVKATRREAKDASLQRKENT
                     EKIAERRRLNQFYRDLIRDSFADLKDYVEHKRKIVARGANPLEDQELDAKRAALLEKV
                     KDAEIHPEEINTFSEKFINAAKKIRKYRKEQERLERILQISSMRELRTLGRNLTVKEK
                     REEIEETLGLSSDEIKEKIRQVQVTEKKLKELELAFESDADQIITMAKEINRGRIMMK
                     NAKDRLIKANLRLVVSIAKKYTNRGLHFFDLVQEGNIGLIKAVEKFEYRKGYKFSTYA
                     TWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPTDEEVAEQLGW
                     PVSRVKSVKNVAREPISLETPIGEDEDSLLGDFIEDKEVENPSVQTDYKLLQEQIRAV
                     LSTLPPREQEVLRMRFGLDDGYSLTLEEVGLYFNVTRERIRQIEAKALKKLRHFKRSQ
                     KLRDYMDH"
     gene            6406..7266
                     /locus_tag="PLG14_02820"
     CDS             6406..7266
                     /locus_tag="PLG14_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOX48093.1"
                     /translation="MEEVFERLREYQEILTKRVEIEKEVEDIPKALSAQEELLSRVKK
                     SYVERREEFEAAEAKVKELRGQLAEAETAREAAEKQMDAISTQREYEALNKEIRDATD
                     REHQFRKDLQHEERTLADVEELMRKDESMIKLQETEIAEKSAKINVDKGAKLAEIGKL
                     RKEEDRSSDGIDRSVIFKFERIIRSKQGLGIVAVKGCVCTGCNMILPAQFVNEVRSGN
                     RIIFCPYCSRILFHQEADEDSGDQIYADIESGSLADLEDYAEEGEEGDDDDDGFEGGD
                     KDSSMDDSDE"
     gene            7279..7844
                     /gene="rnpB"
                     /locus_tag="PLG14_02825"
     ncRNA           7279..7844
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG14_02825"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7974..8957
                     /locus_tag="PLG14_02830"
     CDS             7974..8957
                     /locus_tag="PLG14_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48094.1"
                     /translation="MMESFSRTLDNYTGSYVARRRRKRIIVVTAVLAAAAALAFGIAG
                     LSRKGLLSAGGEGKPVPKKTVLASWGSKDWDEVLAACAASLGAAPLDPFYLGMGGLAA
                     YYKGIELPEGEERSALMDEAVVSIRKALVAHGRSARGTPRAELEYVLGKAYYYKGRDF
                     MDGAVKWLEASIDDGYLGADSREYLAVAYASLGNIEASLKNFEAALQRGRADLLLVAA
                     ARAYAEAGKDDRAESLLLEALSSSQDALAREKCRFGLADIYAGRGEEGKVEEQLGLAL
                     KENAESAEAHYRLGVVYQKRGDPVRARAEWRRAIAIDPMHAASRQKLAEKL"
     gene            8980..10023
                     /locus_tag="PLG14_02835"
     CDS             8980..10023
                     /locus_tag="PLG14_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOX48095.1"
                     /translation="MATRSIFDSFSLDIGIDLGTCNTLIYVRGKGIVVNEPSVVAVER
                     GTNKVVAVGTEAKRMLWKTPGDIIAKRPLRDGVIADPDMTEKMIKYFIQKVISRRFFV
                     KPRMVIGVPSCITKVEENAVVDSAYKAGARFVKFIAESLAAAIGADIPISEPAGHMIC
                     DIGGGTTEISVISLKGMVVTNAIRIGGDEFDEAIIKHIRNVHNLIIGEQTAERLKIEI
                     GNASPDKVIEKVEIKGTDAITGLPRRLEIDSVEVREALQEPVTQIVEEIKKTLGQTPP
                     ELAADIVERGIVMAGGGSLLKGLPKLVAKETGVPVILAERPLECVALGAGKYFETMRS
                     QDGSQSVYDSLNI"
     gene            10028..10942
                     /gene="mreC"
                     /locus_tag="PLG14_02840"
     CDS             10028..10942
                     /gene="mreC"
                     /locus_tag="PLG14_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOX48096.1"
                     /translation="MRGSPKASSPERLRRAVPVVLLAASLVVLTVSTRSLAGLPERVG
                     VTVFGFFQKGFSAVGDFVSDTIASIAELKRLRKDYDELTEKLEAYTNMERGNAELREE
                     NERLKAQLGFSERLSYERVPARIVAKDPENLYATIVIDKGVAEGVRKNMPVIAFQDGV
                     EGLVGRVLEVGMGSSIVVPVYDSSSYVAARLAASRYEGLAKGQGSADSPLVMKYVKKR
                     AKDEAQFGDLVVTTGYESIYPPDVTLGRVKKLRILDYQTSVEIEVEPALDFSRLEYVL
                     VVRPSAPAPAADGEAPAPGGLPAQGAKP"
     gene            10939..11445
                     /gene="mreD"
                     /locus_tag="PLG14_02845"
     CDS             10939..11445
                     /gene="mreD"
                     /locus_tag="PLG14_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOX48097.1"
                     /translation="MIRTIVLSSLLLLLCSFVQSTWLGAVAVLGVVPDLGLLVLIWVS
                     YKNGLVEGPVSGFVAGLAEDAVSAAPLGFNAFVKTLVAASAGLLHGTFYIDRIVLPFV
                     LGAVGTLLKALAAALLALLFGSKVQAYDLLDRALWIEAAYNGLVAPFLFLLLSAFRRL
                     LVTESKRE"
     gene            11442..13316
                     /gene="mrdA"
                     /locus_tag="PLG14_02850"
     CDS             11442..13316
                     /gene="mrdA"
                     /locus_tag="PLG14_02850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOX48098.1"
                     /translation="MRPDTSPGAEQGSLKRRVDLLGLAALCLFVLYSGYLFYLQVVKG
                     NEYRSKARSIARQTTLLPAQRGEIYDRGYQLPLVLNTDSFAVDLIPAELPAERREEVF
                     SRLAAALGVPESEIARRVPPAYYHLYQPVEIAASIPYATVAKVAERIDEFPGLSWHAK
                     PVRNYLETGSLSQVIGYVGDITKDELKLLYNQGYQGGDVIGKAGVEKQYDGLLRGKDG
                     REYRTVDVRGKNVQQERESVVPPVTGNNVVLTIDAKVQRVAEKALGERMGSVVVLKPA
                     TGEVLALVSYPWFNPNLFTGLGAGNAYAKLLADPNTPLLNRAIQSSYPPASTFKTVLT
                     AGVIEEKAFPLDKKILCQGAVSYGDRVFNCHIKRPGHGWLDLEGALAQSCDIYFWQVG
                     RDHLGVERIVSYAKEFGFGRLTGIDLPGEIEGFVPTPQWKERRFHEKWLGGDTMNLSI
                     GQGYMLVTPLQVANMMAMVVNEGTVYRPHVLKELRDPASGAVARSVEPEVILRSRISP
                     DTWDLVRKDLRTVVTSGTVRFPISTKAVEVAGKTGTAEVGLKDRWHSWFASYGPSDAA
                     PEDAIVVVVMVEASNPWEWWAPYASNIIYQAVFAGQEYDEAVDALGLRYLVPRQERQE
                     "
     gene            13313..>14306
                     /locus_tag="PLG14_02855"
     CDS             13313..>14306
                     /locus_tag="PLG14_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOX48099.1"
                     /translation="MSSLKASMAEIDLAALGAALGLVVIGVLSIYSAGVTAEGVLVSK
                     EYLKQILWALSGLALMVLMVAMDYKRWKDYSVFLYAFFMLVLVYTRLFGRVVNGARSW
                     IGIGEVGLQPSEFTKVAAILFLAQYLEASEHQSSIRRLFVSFSIVLLPVGLILSQPDF
                     GTALVFFPILIFMIFVAGLDKRYIVFILAAILLTFFLLVLPLWERYILPKPTAFLFVL
                     YREPYCYLLIGAAAFVLALSAWGWISYKKGYYFWMAYVSLLVVGSLGASVLAHKVLKE
                     YQVMRLIVFLDPSIDPRGSGWNILQSITAIGSGGLAGKGFLQGTQSHYRYLPQQS"
BASE COUNT         2458 a         5172 c         4457 g         2219 t
ORIGIN      
        1 tcccctgggg agattcccga gtggtcaaag ggggcagact gtaaatctgt tggcttacgc
       61 cttcgaaggt tcaaatcctt ctctccccaa gagccccctc tcgcttcgag aggggttttt
      121 tttgccttag gagggtcgcg gtgggcgatg ttttctacgg cgagggcctg cgtttctcct
      181 gcgcccgctg ttcctcctgc tgccggggcg ggcccggcta cgtcttcctc tcgaaagagg
      241 acctaaagcg tctcctcctc cgcacttccc ttgcttttcc cgcctttttc gccgaatact
      301 tgacgacggt ggacacgggg acgggctacg ccctgagcct cagggaaaag cccaactacg
      361 actgcatctt ctggggagag tccggctgcg ccgtctacga ggacaggccc gtccagtgct
      421 ctaccttccc tttctggtca tccattctcg cgtccgaaga ggactggcgg gacgccggtc
      481 gcgattgtcc cgggataggc cgcggcgagc tccgctcgcg cgcgtatatc gaagagcgcc
      541 tccatgcgag gcgggccgcg ggcacgctcc tccttacgaa ggacgaggcc gcgcatccgg
      601 agaggataga tgcggatacg cttctgggga tgtaggggct ccctgccgag ccccctcgtg
      661 ccggccaagc ttcggagcaa gatctcctcg gtcatcggcc gcgtcggccc cgccgacata
      721 gcgagcccgg aggcccggga gcgcttcctc gccggccttc ccgaatacct cttctccacc
      781 gtgggcggca acacgccctg cgtggagata gcgaccgagg agcccgagag gctcgtcttc
      841 gacgccggca ccggcatccg cgagctcggc aacgagatcg tcgccgccaa gaagcccgtc
      901 tcctgccacc tcttcttctc ccacttccat tgggaccaca tccagggcct gcccttcttc
      961 ggcccggcct acgacccgag gaacgagctc ttcttccaca gccccgtgga gggcttccgc
     1021 tccatcctcg agggacagat gcgccagccc tatttcccca tcacgatgga ccagatgtcg
     1081 gcccgcaagg acttcaccgt cctggacggc aagcccttgc ggctcggcgg ggtcgaggtg
     1141 tcctacaggc ccatgagcca tccaggcggc tgctactgct accgcgtgga ggagaagggg
     1201 cggaaggcca tctactccac cgacaccgag ctctccgagg ccgacttccg caagagcccc
     1261 gagaacgagg ccttcttccg gggggtggag ctcctcgtca tcgacaccca gtacacgctg
     1321 ggcgaggcca tcgagaagta caactgggga cattcctcct tcagcctcgc ggccgacttc
     1381 gccgccacct ggggcatcga gcggctcgtc ctcttccacc acgagccttc ctattcagac
     1441 cgcaagatcc acggcaacct caagaacgcc tcctggtacc tcgaccatct ggagagccgg
     1501 ggcatcgagg tcatcctggc ccgcgagggc ctcgaggtcg aggtctaggc cgtggaggac
     1561 ccccgcgccg ccgatgccga cttccgtccg gccctgggcc tcaaggcctc cctcgccctc
     1621 ttcctcctcc tggaagggcg ctcggacggc atagccggct cggcccccga gctcgaccgc
     1681 ctggaggacg agctcaggga ctacctctac ggaaacctgt cgatagccga catggagaat
     1741 cccggaaaga cctattcggc cctggccggg cggaaagaga gagcatgaag aagtcccccc
     1801 gccgcctggc ccttgtcctt tcgctggccg tcggcctcct cgccggcctc ctcgccgcgg
     1861 gcttcgccgc cttcgccctg gcccttcgcc cccccgcggg cctggccgag gaggggctcc
     1921 tcttcacggt cagggcgggg gagggcgccg ggatcctcgc ggcccgactc gaggccgagg
     1981 gggccatacg ctccgcgctc gccttccgcc ttctggccaa ggcgaggggc gacgagggca
     2041 ggctcaaggc cggcgtctac cggatcgaac ccgcgatggg cggcagggag gtccttggcg
     2101 ccatcgtctc gggcaggcag gccctggtcc gcctcacggt tcccgaaggc gcgaccctct
     2161 cccgcgtcgc cctcatcctg gaggaggggg gcgtggcggc ggcggcggaa ttccgggcgg
     2221 ccgcccggga tccggccctc ctttccgagc tcgggatccc aggcccctcc ctggagggct
     2281 acctcttccc cgacacctat ttcatgcccc tgggctacgg cgccgcgggg gccgccaggg
     2341 ccatggcggc ggccttccgc tcccgcctcg cgtccatccc cgaagccgcc gcgctgtcgc
     2401 ccgaggagct ccgcgagaag atcatcctcg cctcaatcgt ggagcgggag taccgggtcg
     2461 ccgacgaggc tcccgtgatg gcgagcgtct tctacaaccg cctccgcatc ggcatggccc
     2521 tccaatcctg cgcgacggtc gtctacgtga tcaccgagaa gctcgggaag ccccatcccg
     2581 agcgcctttt cgaccgggac atcgcgattc ccgacgccta caacacctac ctggcccgcg
     2641 gcctcccgcc ggggcccatc gccaacccgg gaatgacggc gatccgctcg gtcttttatc
     2701 ccgcgagcac caagtacctc tatttccgcc tcgtcgacga gtcggccggc cgccaccact
     2761 tctccgagac cctggaggag cacaacgacg cccggtccct cttcgtgaag cgggccggag
     2821 ggaaatgagc ctcatacccg aagcgacggt caggaccatc ctggaccgga ccgacatagt
     2881 cgccgtcatc ggcgactatg tccggctcga gaagagggga gggcgctggc tcggcctctg
     2941 ccccttccac gccgagaaga cgccctcctt caacgtcgac cgcgagaagg gcttcttcta
     3001 ttgcttcggc tgcaggaagg gcggcgacgt cgtcaccttc ctccgcgagc acgagaagat
     3061 gggctaccgc gaggccctgg aggagctcgc taggcgcgcg ggcgtcccca tcgagctcga
     3121 ggaggcccct tccgaggagg agcgggcgcg caaggccctc ctcgagctct acgagcgcct
     3181 ggccgcggcc ttccactacc ttctcgtgga gcacccctcg ggcgaggcgg cccgggccgt
     3241 cctccgccgc agggccatcc ccgacgagct gcgcgaggaa ttccgcctcg gctacgcccc
     3301 ccgcgaccgc cgctggctcc acggctttct cctcaagaag ggctattccg ccgactttct
     3361 ctcccgctcc ggcctcttcg ccgagcgcca ccccgacttc cccctcttcg ccgacaggct
     3421 catcttcccc atagcccagg cccgcgggga ggtcatcgcc ttcggcggcc gcctcctcga
     3481 gggcgaggga cccaagtaca tcaacagccc cgacacggcc ctcttccaca agcaggagaa
     3541 cctcttcgcc ctcgacaagg ccctgccggc catcaagaag gaaggcgcgg ccctggtctg
     3601 cgagggctac atggacgccc tctccttcca cgccgccggc ctccgcttcg ccgtggcccc
     3661 cctgggcacc gccttcacgg aacggcaggc caagctcctc aagcgttggg cctcccaagt
     3721 cgtcctggcc ttcgacgccg acccggcggg acagcgcgcg gccgagaaat cggtcgtcct
     3781 ggcggccgcg gccggcctgg agccgagggt cgccgtcctg cccggcggca aggacgcctc
     3841 cgaaattctc gaaaaagagg gccccgagtc gttgcaaaag cttcgtgatt tgactataaa
     3901 tggaagcgaa tttctcgtgc gccgggcgag ggagctcttc gacatcggca gcgtggaggg
     3961 caaagcgaag gcggcggcgt tcctgaaccc ctacgccgaa gccctggatt cggaggttaa
     4021 gcgggatgcc ttcttcgatc tcgcgtcccg ggaactgaaa gccgatcccc gttcgctccg
     4081 atctgactac gaagacgcga agaagggcat ttccacccgc aggaccgcca agggcgcctc
     4141 tcccgagggc tcgggtccca tgccggcctc acgcacgccc gacctcctct tcatggcagc
     4201 cgtagcgacg aacgcggacc tcttcggggg ggtcaggtcc ctcctgtcgc tcgaggagct
     4261 cgacgatccg cgggcgcgcg acctctacat cgccctggag gagtgcttca gggccgagga
     4321 gaccggcctg gactcgatcc tggggcggat cacggacgag cccctccggg tcttcgtctt
     4381 cgagcgcgcg gccacggggg agttctcgga gaatgccgag cgcttcgtgg aggacgggct
     4441 caaggcgctc aggctccgga gcctggagcg gcggagggcc aaggtgctcg agaggatggc
     4501 cgatttccgg gaaaccggct cggaggcctg gacggagacc gaagggcctt cgctcaacga
     4561 ccttatgtat gagaagatgt acctggacgc cgaattggcg aagataaagg gtgagcggga
     4621 tgaacgatct tgaactagat ccggcgatag cgaagctcct cgaatacgcg aaggccaaga
     4681 agtccatcag cttcgacgag ctttccgatt tcctgccgga gcacgtcctc aactccgaga
     4741 agatagacgg catcctcgcc ctcctggaga gcaacaacat ccagctcgag gaggaggagg
     4801 tcgccgtcga ggacgaggcc cccgtcaagc aggagaccga gaagaagcgc ctcgtctaca
     4861 acgacaagga atcctcggta gacgacccga tccgcctcta cctccgcgag atcggcaagg
     4921 agaacctcct caccgccgag caggaggtcg agctctccaa gtccatggag gagggcgaga
     4981 acatcatcaa ggggatcatc aagcgctccg gcgtcctgat ctccgagttc cacctcatcg
     5041 ccgtgaaggc gacccgccgc gaggccaagg acgcctccct ccagcgcaag gagaacaccg
     5101 agaagatcgc cgagcgccgg cgcctcaacc agttctaccg ggacctgatc cgcgactcct
     5161 tcgccgacct caaggactac gtcgagcaca agcgcaagat cgtcgcccga ggcgccaacc
     5221 ccctggagga ccaggagctc gacgcgaagc gcgccgccct cctggagaag gtcaaggacg
     5281 cggagatcca tccggaggag atcaacacct tctccgagaa gttcatcaac gcggccaaga
     5341 agatccggaa gtaccgcaag gagcaggagc gcctggagcg catcctccag atctccagca
     5401 tgcgcgagct ccgcaccctg gggcgcaacc tcacggtgaa ggagaagcgc gaggagatcg
     5461 aggagaccct gggcctctcc tccgacgaga tcaaggagaa gatccgccag gtccaggtta
     5521 cggagaagaa gctcaaggag ctggagctcg ccttcgagtc cgacgccgac cagatcatca
     5581 cgatggccaa ggagatcaac cgcggccgca tcatgatgaa gaacgcgaag gaccgcctca
     5641 tcaaggctaa cctccgcctg gtcgtctcca tcgccaagaa gtacacgaac cgcggcctcc
     5701 acttcttcga cctggtgcag gaaggcaaca tcggcctcat caaggccgtg gagaagttcg
     5761 agtaccgcaa gggctacaag ttctccacct acgcgacctg gtggatccgc caggcgatca
     5821 cgaggtcgat ctccgaccag gcccgcacga tccgcgtccc cgtccacatg atcgagcaga
     5881 tcaacaaggt cgtccgcgag tcccgccagc tcatgcagaa gctcgggcgc gagccgacgg
     5941 acgaggaggt ggccgagcag ctgggctggc ccgtctcccg cgtgaagagc gtgaagaacg
     6001 tcgcccgcga gccgatctcc ctggagaccc ccatcggcga ggacgaggac tccctcctcg
     6061 gcgacttcat cgaggacaag gaggtggaga acccctccgt ccagaccgac tacaagctcc
     6121 tccaggagca gatccgcgcc gtcctctcga ccctgccccc gcgcgagcag gaggtcctgc
     6181 gcatgcgctt cggcctggac gacggctact ccctcaccct cgaggaagtg ggcctctact
     6241 tcaacgtcac ccgcgagcgc atccgccaga tcgaggccaa ggcgctcaag aagctccgcc
     6301 acttcaagcg gagccagaag ctccgcgact acatggacca ctagccgctc cggcggctag
     6361 ggacggcaac gtcattatac ccccgatgcg aggtgccata tgctgatgga agaagtcttc
     6421 gagcgtctcc gcgagtacca ggagatcctc accaagcgcg tcgagatcga gaaggaagtc
     6481 gaggacatcc cgaaggccct ctccgcccag gaggagctcc tctcccgcgt caagaagtcc
     6541 tacgtcgagc ggcgcgagga gttcgaggcc gcggaggcca aggtcaagga gctgcgcggc
     6601 cagctcgccg aggccgagac ggcgcgcgag gccgccgaga agcagatgga cgccatctcc
     6661 acccagcgcg agtacgaggc cctgaacaag gagatccgcg acgcgaccga ccgcgagcac
     6721 cagttccgca aggacctgca gcacgaggag cggaccctcg ccgacgtcga ggagctcatg
     6781 cgcaaggacg agtcgatgat caagctccag gagacggaga tcgccgagaa gtcggccaag
     6841 atcaacgtcg acaagggagc caagctcgcc gagatcggca agcttcgcaa ggaggaggac
     6901 cgctcctccg acggcatcga ccggagcgtg atcttcaagt tcgagcgcat catccgctcc
     6961 aagcagggcc tcggcatcgt ggccgtgaag ggctgcgtct gcaccggctg caacatgatc
     7021 ctgcccgcgc agttcgtcaa cgaggtgcgc tccggcaacc ggatcatctt ctgcccctac
     7081 tgctcccgca tcctcttcca ccaggaggcc gacgaggatt cgggcgacca gatctacgcg
     7141 gacatcgagt ccggctcgct cgccgacctc gaggactacg cggaggaggg cgaggagggc
     7201 gacgacgacg acgacggctt cgaggggggc gacaaggact cctcgatgga cgattcggac
     7261 gagtgaacta ggttattgag ggcgggccgc gccaccgtcg cgccttcggc cctcgaaagg
     7321 cccttcgggg cccggagagg ccggcggcgc ggaggaaagt ccgggctcat gagagcgcag
     7381 cgccgggtaa ctcccgggcg ccacgacgaa ggcccgctcc gagaggagcg ggtacccgcc
     7441 ggggcgacag acagcgcaac agaaagatac cgcccggcca ggacccgtcc ttccgatacc
     7501 cgcggggcgc gaaagcggcc cgcgccgtcg gcggggcgga aagccaggcc gggtaagggt
     7561 gaaatggcgg ggtaagagcc caccgcgcct ccggcgacgg aggcggcacg gcaagcctcg
     7621 ctgcgagcaa ggccgagcag cggagggcga ctcgcccttc gtcggagttc cgagggtcca
     7681 cggcgagaag ttgcccgcaa gggcaacttc aacgtcgagg cccgaagggc ctcgacgaga
     7741 tccgcgggta ggccgcgcgg gatccgccgg gcgaccggcg gccgagatgg atggtggcgg
     7801 ccccgaaagg ggtacggaac ccggcttata ggcccgccct tttttttatc gttcccgcgg
     7861 gcgccaaacg cgccccgagg gccgcggtcg atgccgaggg cgaaggcctt cgacattgac
     7921 ggcggccctt ccgccatggt acaaggtatg cggcgctcgg gcgccggcgg catatgatgg
     7981 aatcattctc caggaccctc gacaactaca ccggaagcta tgtcgccagg cgcaggcgca
     8041 agcggatcat cgtcgtgaca gccgtcctcg cggccgcggc ggcgctggcc ttcggcatcg
     8101 ccggcctctc gcgcaagggc ctcctctcgg ccggcgggga gggcaagcca gtccccaaga
     8161 agaccgtgct cgcgagctgg ggctccaagg actgggacga ggtcctggcc gcctgcgcgg
     8221 cctccctggg cgccgcccct ctcgacccct tctacctcgg catgggagga ctggccgcct
     8281 actacaaggg catcgagctt cccgagggcg aggagaggtc ggccctcatg gacgaggccg
     8341 tcgtctccat ccgcaaggcc ctcgtcgccc acggccgctc ggcccgcggc accccccgcg
     8401 ccgagctcga gtacgtcctc ggcaaggcct actactacaa gggccgcgac ttcatggacg
     8461 gcgccgtgaa gtggctcgag gcctccatcg acgacggcta cctgggcgcc gacagccgcg
     8521 agtacctcgc ggtcgcctac gcctccctcg ggaacatcga ggcctccctc aagaacttcg
     8581 aggccgccct ccagcgcggc cgcgccgacc tcctcctggt cgcggccgcc agggcctacg
     8641 ccgaggccgg gaaggacgac cgcgccgagt ccctcctcct cgaggccttg tcctccagcc
     8701 aggacgccct ggcccgcgag aagtgccgct tcggcctcgc cgacatctac gcgggccgcg
     8761 gagaggaggg caaggtcgag gagcagctcg gcctggccct caaggagaac gccgagtccg
     8821 ccgaggccca ctaccgcctc ggcgtcgtct accagaaaag gggcgatccc gtccgcgccc
     8881 gcgccgagtg gcgccgcgcc atcgccatcg accccatgca cgccgcctcg cggcagaaac
     8941 tcgcggagaa gctctaaacc ccgacggagg tatcctctca tggccacgcg ctcgatattc
     9001 gactccttct cgctcgacat cggcatcgac ctcggcacct gcaacaccct catctacgtc
     9061 cgcgggaagg gcatcgtcgt gaacgagccc tccgtcgtcg ccgtggagcg cgggacgaac
     9121 aaggtcgtcg ccgtcggcac cgaggccaag aggatgctct ggaagacccc cggcgacatc
     9181 atcgcgaagc gccccctgcg ggacggcgtc atcgccgacc ccgacatgac ggagaagatg
     9241 atcaagtact tcatccagaa ggtcatctcc cgccgcttct tcgtgaagcc gcgcatggtc
     9301 atcggcgtgc cctcctgcat cacgaaggtc gaggagaacg ccgtcgtcga ctccgcctac
     9361 aaggccggag cccgcttcgt gaagttcatc gcggagtccc tggccgccgc catcggcgcc
     9421 gacatcccca tctccgagcc cgccggccac atgatctgcg acatcggcgg cggcacgacg
     9481 gagatctccg tcatctccct caagggcatg gtcgtcacga acgcgatccg catcggcggc
     9541 gacgagttcg acgaggccat catcaagcac atccgcaacg tccacaacct catcatcggc
     9601 gagcagacgg ccgagaggct caagatcgag atcggcaacg cgagccccga caaggtgatc
     9661 gagaaggtgg agatcaaggg cacggacgcc atcaccggcc tcccgcgccg cctggagatc
     9721 gactccgtgg aggtccgcga ggccctccag gagcccgtca cccagatcgt ggaggagatc
     9781 aagaagaccc tgggccagac cccgcccgag ctcgccgccg acatcgtgga gcgcggcatc
     9841 gtgatggccg gcggcggctc cctcctcaag ggcctcccca agctcgtcgc caaggagacg
     9901 ggcgtccccg tcatcctcgc cgagcgcccc ctcgagtgcg tcgccctggg cgccggcaag
     9961 tacttcgaga ccatgcggag ccaggacggc tcgcagagcg tctacgacag cctgaacatc
    10021 taggacgatg cgcggctcac cgaaggcgtc gagccccgag cggctgaggc gggccgtgcc
    10081 cgtcgtcctc ctggccgcga gcctcgtcgt cctgaccgtg tcgacgagga gcctcgcggg
    10141 gcttcccgag cgcgtcggcg tcaccgtgtt cggcttcttc cagaagggct tctccgcggt
    10201 gggggacttc gtctccgaca ccatcgcctc catcgcggag ctcaagcggc tccggaagga
    10261 ctacgacgag ctcacggaga agctcgaggc ctacacgaac atggagcggg gcaacgcgga
    10321 gctccgcgag gagaacgagc ggctcaaggc ccagctcggc ttctccgagc ggctctccta
    10381 cgaacgcgtc cccgcccgca tcgtcgcgaa ggatcccgag aacctctacg cgacgatcgt
    10441 catcgacaag ggcgtcgcgg agggcgtgcg caagaacatg ccggtgatcg ccttccagga
    10501 cggcgtggag ggcctcgtgg gccgggtcct cgaggtcggc atgggctcga gcatcgtggt
    10561 gcccgtctac gactcgagct cctacgtcgc cgcccgcctc gcggcgagcc gctacgaggg
    10621 cctggccaag ggccagggct cggcggactc gcccctcgtc atgaagtacg tgaagaagcg
    10681 ggccaaggac gaggcccagt tcggcgacct tgtcgtcacc accggctacg agtcgatcta
    10741 cccgcccgac gtcaccctgg gccgcgtgaa gaagctcagg atcctcgact accagacctc
    10801 cgtggagatc gaggtcgagc cggccctcga cttctcccgc ctcgagtacg tcctcgtcgt
    10861 gcgcccctcc gctccggctc cggcggccga tggcgaagcg cccgcgcccg gaggcctgcc
    10921 ggcccaagga gccaagccgt gatccggacc atcgtcctgt cctcgctcct actcctcctc
    10981 tgctccttcg tccagtccac ctggctgggc gccgtcgccg tcctgggcgt cgtgcccgac
    11041 ctggggctcc tcgtcctcat ctgggtctcc tacaagaacg gcctcgtcga ggggcccgtc
    11101 tccggcttcg tcgccggcct cgcggaggac gcggtctcgg ccgcgcccct gggcttcaac
    11161 gccttcgtga agaccctcgt cgccgcctcc gccggcctcc tccacggcac cttctacatc
    11221 gacaggatag tcctgccctt cgtcctggga gcggtcggca ccctcctcaa ggccctggcc
    11281 gcggccctcc tcgccctcct cttcggctcc aaggtccagg cctacgacct cctggaccgc
    11341 gccctctgga tcgaggcggc ctacaacggc ctcgtcgcgc ccttcctctt cctcctcctt
    11401 tccgccttca ggcgcctcct cgtcacggag tcgaagcgcg aatgaggccc gacacctccc
    11461 cgggagccga gcagggctcc ctgaagcgcc gcgtcgacct cctcggcctc gcggccctct
    11521 gcctcttcgt cctctactcg ggctacctct tctacctgca ggtcgtgaag ggcaacgagt
    11581 accgctccaa ggcgaggagc atcgcaaggc agacgaccct cctgcccgcc cagcggggcg
    11641 agatctacga ccgcggctac cagctccccc tcgtcctcaa cacggattcc ttcgccgtcg
    11701 acctcatacc cgcggagctg ccggccgagc gccgcgagga ggtcttctcc cgcctggccg
    11761 ccgcactggg cgtgccggag tcggagatcg cgcgccgcgt gcctcccgcc tactaccacc
    11821 tctaccagcc cgtggagatc gcggcctcca ttccctacgc cacggtcgcc aaggtggcgg
    11881 agcgcatcga cgagttcccc ggcctctcct ggcacgcgaa gccggtgcgc aactacctgg
    11941 agacgggctc cctctcgcag gtgatcggct acgtcggcga catcacgaag gacgagctca
    12001 agctcctcta caaccagggc taccagggcg gcgacgtcat cggcaaggcc ggcgtcgaga
    12061 agcagtacga cggcctgctc cgcggcaagg acggccgcga gtaccggacg gtggacgtcc
    12121 gcggcaagaa cgtccagcag gagcgcgaga gcgtcgtgcc gcccgtcacg gggaacaacg
    12181 tcgtcctcac gatcgacgcg aaggtccagc gcgtcgcgga gaaggccctg ggcgagcgca
    12241 tgggatccgt cgtggtcctc aagcccgcca cgggcgaggt cctggccctg gtctcctatc
    12301 cctggttcaa ccccaacctc ttcacgggcc tgggcgccgg caacgcctac gcgaagctcc
    12361 tggccgaccc gaacacgccc ctcctgaacc gcgcgatcca gtcgagctac ccgccggcct
    12421 ccaccttcaa gacggtcctc accgcgggcg tcatcgagga gaaggccttc cccctggaca
    12481 agaagatcct ctgccagggc gccgtctcct acggcgaccg cgtcttcaac tgccacatca
    12541 agcggccggg ccacggctgg ctggacctgg agggcgccct ggcccagtcc tgcgacatct
    12601 atttctggca ggtcggccgc gaccacctgg gcgtggagcg catcgtctcc tacgcgaagg
    12661 agttcggctt cggcaggctc acgggcatcg accttcccgg cgagatcgag ggcttcgtcc
    12721 cgactcccca gtggaaggag cggcgcttcc acgagaaatg gctcggcggc gacacgatga
    12781 acctctccat cggccagggc tacatgctcg tcacgcccct gcaggtggcc aacatgatgg
    12841 cgatggtcgt caacgagggg actgtctaca ggccccacgt cctcaaggag ctccgcgacc
    12901 cggcctccgg cgccgtcgcc cgcagcgtcg agcccgaggt catcctgcgc tcccggatct
    12961 cccccgacac ctgggacctc gtcaggaagg acctccggac ggtcgtcacc tccggcacgg
    13021 tgcgcttccc gatcagcacc aaggcggtgg aggtggcggg caagacgggc acggcggagg
    13081 tggggctcaa ggaccgctgg cattcctggt tcgcctccta cggaccgtcc gacgccgccc
    13141 ccgaggacgc catcgtcgtc gtcgtcatgg tggaggcctc taacccctgg gaatggtggg
    13201 cgccctacgc ctccaacatc atctatcagg ccgtcttcgc cggccaggag tatgacgagg
    13261 ccgtcgacgc cctcggcctc cgctatctcg tcccgcggca ggagcgccaa gaatgagctc
    13321 cctcaaggct tctatggccg agatcgacct ggccgcgctc ggggcggccc tgggcctggt
    13381 cgtgataggc gtcctctcca tctactccgc gggcgtcacc gccgagggcg tcctcgtctc
    13441 caaggagtac ctcaagcaga tcctctgggc cctctccggt ttggcgctca tggtcctgat
    13501 ggtggccatg gactacaagc gctggaagga ctactccgtc ttcctctacg ccttcttcat
    13561 gctggtcctc gtctacacga ggctcttcgg ccgcgtcgtg aacggggccc ggtcctggat
    13621 cggcatcggc gaggtgggcc tccagccctc cgagttcacg aaggtggcgg ccatcctctt
    13681 ccttgcccag tacctggagg cctccgagca ccagagctcc atccgccgcc tcttcgtgtc
    13741 cttctcgata gtcctccttc ccgtcggcct catcctctcc cagccggact tcggcaccgc
    13801 cctcgtgttc ttccccatcc tcatcttcat gatcttcgtc gcggggctgg acaagcgcta
    13861 catcgtcttc atcctcgccg ccatcctcct gaccttcttc ctcctcgtcc ttccgctctg
    13921 ggagaggtac atcctcccga agcccacggc cttcctcttc gtgctctacc gcgagcccta
    13981 ctgctacctc ctgatcgggg cggccgcctt cgtcctggcc ctctcggcct ggggctggat
    14041 ctcctacaag aagggctact acttctggat ggcctacgtc tccctcctcg tcgtcggctc
    14101 cctcggcgcc tccgtcctcg cgcacaaggt gctcaaggag taccaggtca tgcgcctcat
    14161 cgtcttcctg gacccgagca tcgatccccg cggctcgggc tggaacatcc tccagtccat
    14221 caccgcgatc ggctcgggcg gcctcgctgg caagggcttc ctccagggca cgcagagcca
    14281 ctaccgctac ctgccccagc agagca
//
LOCUS       DAOOSJ010000031        14301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2856_length_14301_cov_6.192264, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000031 DAOOSJ010000000
VERSION     DAOOSJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_14301_cov_6.192264"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..553)
                     /locus_tag="PLG14_02860"
     CDS             complement(140..553)
                     /locus_tag="PLG14_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48100.1"
                     /translation="MDKRISICDRGPAKASSVAAILAAGLALSACSPLKQSAIQGSLY
                     GEKGAATSLELAAPDARLSVSGRVELEAGGASVELFEPSGALAWRSEYLAGEGPSSFP
                     VGPYERRGLSGTWILSVKGTAAEARGGYDLMLSNR"
     gene            700..1431
                     /locus_tag="PLG14_02865"
     CDS             700..1431
                     /locus_tag="PLG14_02865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX48101.1"
                     /translation="MIPALYQDEEILVLDKPAGLASQPGAGVGLSLVEAVERDYGFRP
                     FLVHRLDKETAGCILVAKSSQAAARWTADIASRSVGKFYRAVVSGSLEGRRGRIADPV
                     PVRGEQKAGETFWRLVGRFGAAGPGGALGGAPDGGPEAAGGKAAPFAPPGGYSYLELE
                     LGTGRTHQIRLHLAGSGAPILGDDRHGDFALNKRLRKELGLRRLLLVAWRLELPGGLS
                     VRASVPDHLRAFLAAFPDAPDPEAP"
     gene            1428..1823
                     /locus_tag="PLG14_02870"
     CDS             1428..1823
                     /locus_tag="PLG14_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48102.1"
                     /translation="MTDARPVPELRHGASWLTGDEELIPVPGFHEDWIGSHRDLVGEA
                     KNVCDVVLGLGWISVSVFSEGYVELLVPNRRDPVLRERIGRLLRGNGGRWRRVLVMSM
                     DEEGYASLGPGDLEVPGRLEALLSGATSA"
     gene            complement(1810..6471)
                     /locus_tag="PLG14_02875"
     CDS             complement(1810..6471)
                     /locus_tag="PLG14_02875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HOX48103.1"
                     /translation="MQRAPRRFPRALILLLGLLGVAARATAQDYYWETPRALVEGSGR
                     FPTALSSSGGVLVVWQESEPVPGGDAGRAWLSLARLSGGSWETRRRFAGPYEFRGSEP
                     LLYSASSEDGQVAIAAAAGDGAIEVLLSRDGGVSFERTERPAPSAAAVAPRVFPSASG
                     GWLLFATQGEAESLSLFYATSRDGSSWSPFAPFVGAGDGLALNFLPTAARSRDSGGPV
                     DVVVFQSLLPGARRSFQLFSKVSRDGGATWSAARRVTDFPDPVVRERNEADNFDNQRP
                     FLAEAAGKLWLAWERATLLGQPQVYAMTLDPSGAAQSATADRVSSGAGSCSAPQVLEL
                     DGRPAVVWFDNRRGGNRVYLVARKGLDWPESELSGSRGDASFGRIAVSDGKPYVFWQS
                     TEARRDRILALEPDTSVAPPALEALDWRPGVATRRETASARVQLAPDSSGVEGYSYLW
                     SRDQAAEPPRKLMALPDQARISLSAPEDGAWWLAVAAVDYAGNWSRAARLRFDRDRTP
                     PPPPLLQPPPQDEGGFLSSNSFAVRWAPPEADDVAGYTWELRYVGGLERSAAALPAGP
                     RAAARPPLPGLSPYEALLVERAGPPVPPPALLGAEPRASYANVDDGYYLFSVASVDRT
                     GNIGDAATILLRADKYVPYTTVGYVDARRDDLGRTSLRLLGRGFAAEGLAATVVLDRD
                     GREPYDLVRERSDGGFRVASDREIDGLAFEGLEAGTYRVGVLHPKRGWYWSGALLSVD
                     ASGTLKFGASGGEYVPSWRILERRRYRFSIYDAIVAAAVLFAGLGIALSLAQVRAVVL
                     DGEAVRREVLALVSGGPMPSGSTAQVARALRRRGTGLRAKFTLIIALLVIFVVLLLSV
                     SLGAFMIRTESTNLARGLEQRSLVLLESVAQGGRSYLPAQNVLELGFLPQQAKAMEDA
                     AYLTLTGYGAEASTDPDVVWASNDPGIGGKIDGAELKQGSSRLSDALTPSLAGIAKEI
                     NDRAALEVGSLNETLAAYTQEGRALATKLDAASQRRLAEIADTSRGLERELNAKLFAL
                     ANASVGSIPAFDPETALRGGGRFLFYKPILYRRGSDQLYYRGMVRLEVTTDIIAADVR
                     KATAELIRITLIIAAVALAIGVAGAILLSSIIVGPIKKLEKAITRIRDEKDKEKLGDI
                     EELKGFKGSKSRDEIFVLADAVGQMASGLAKAAKASKDLTLGKDIQKMFIPLEVGPKG
                     SKLTTGSLSEKAFEFFGYYEGAKGVSGDYFDFNRLDSRYYYFVKCDIAGKGVAAALIM
                     VEVATMVINHFDGRDVAKKGPELESLAYQINGFIEGRGYKGRFAALTLGIYDAETGVA
                     RLCHAGDRLCHVWRDAARRMEIIELPDAPTAGTFPNMLVEMKTPYRQVPVKLEKDDIL
                     LLYTDGIEEAKRHFRNEKLEIVPCADAEKDKPHDSHHSGGQDNEEFGYDRLCEILAAV
                     DAKGSFRLVKAHDPNPDAVLTFDFSTCGETLEERVVALIAVEKVYRMYPDPDAGESDV
                     VLADRKVDAFLEKHFDQYRLYCSRKRDNPDPEHPEYVLYAGIREDEQYDDLTVLGIRR
                     K"
     gene            complement(6504..9560)
                     /locus_tag="PLG14_02880"
     CDS             complement(6504..9560)
                     /locus_tag="PLG14_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48104.1"
                     /translation="MSVPPSRSPRARASRLLLLILAAWTVLGVPAAFGMGKKEDPLRE
                     ADTLIAGQRYNEAILYLTEFIKKYPDRFDDAQAKLRKVIELRESYNQAGKDLIDTLVN
                     NPLDEKAKLDAIARLQSIDPNDPGTKAFIERTKLASLFVYNRAQFDAIMAKGRTLIEA
                     GRYVDAARAYEEGFVLYRDEFDRGPYDQLTKQSVAGLVDRVKTEIVVYASSQASLVPV
                     VERLRAAFAAGDPAAAESAWPAAEAALLERARRRNAVVAAGRSMQRQFEVIQAADRTA
                     TDSSFLPFAFRFTLGRTTAALPEGISGAMDNQWIALLNGMQAALDGRIDEYAAAAEAA
                     YDAGRWEEAKTGFERTAALAEPGLRALGLWSLVAPTEILPEPTQYGRAVLAGKAAAYE
                     RIRHLGKAAAAQARIAAIAGRAAAYDEEARRFAESLPDDAPVEPTLAGFAARRKEIAA
                     AGDGLSAEREPARALAEELARWTAAGYGDDRAARVQGSYDGRLAAAVDTLRGYEVSIA
                     ARASGLEYGRFEADLASRKAAVAEGRRLLEGLPQEGAAEVLLRYPGRSAELLGAEEAS
                     LKALRSRLAEYLRRLERETPYVAGAPSVQLWAEKARGLDREAVALETERLAVAARALD
                     QKRAADSARLEAERRAAEARRALAAEDYETARDRLTKAREKYLASFALEDNAALRAET
                     DRSLQLLGESIVKAENDRVIRDTRRLITEGKNFYFQGVFDRSEDSLLRARARWKTTHG
                     DEPEPEVEYWLKLVQAALSVKTGRDIPATAPLYPEMSQLLSLAKRYYEEGKSLLDRRQ
                     KTDALQYFALSKQKINEVKVVFPLNQEAGVLALKIDQLTDPEAFRSGFGAKFVQARNR
                     LDTEPREAYSELQDLAAIDPKYPGLKAALDRAEILLGIRLPPPDPAKARRAAELVAAA
                     RRVVDSGDTGRFTFALEQLNEAILLDPNNETAVALKDRILTFQGGTAQIVLSSYAEEQ
                     YRAAVEAFQGGNYLQALATVERLLQDPKNKKSQKLLDLYKKIQARL"
     gene            9723..10958
                     /locus_tag="PLG14_02885"
     CDS             9723..10958
                     /locus_tag="PLG14_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HOX48105.1"
                     /translation="MKRSRLLPLLLLALVPAARGAAQLAPSVYQAISEYFNPDPNAGL
                     TAFRSLLIPMGGLAEGMGMAYTAVAKDSSYFESNPAASSGLGQTELSVFHNNWIADTR
                     IEGAVYTIRYKNLGFGAGGKWLYLPFTAYDKYGDREGTGYYSEATAAFNVSYNFLAGY
                     YFNGLALGATGKASYRSVPAAIAEAAGNSTAAVMVDLGALSRFNLLKFYNSREKNFSV
                     GLALKNLGPPSQGEPLPTVASFGLAYSPLRPLLFSLDVSKPINLVEIAKSERPSYGAG
                     FEVRMTDFFGLHGGFLLKGGNPRLSVGSSFDIELVKVVVNYTLDLTTQLTPLNRISVQ
                     ASFSLGDLGRAELAKKVENLYLKGLEAYAGGDSAAAMAAWTEVLKLDSGFDPARESLR
                     AAQGATDLQKTIEELQKLD"
     gene            10975..11568
                     /locus_tag="PLG14_02890"
     CDS             10975..11568
                     /locus_tag="PLG14_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOX48106.1"
                     /translation="MVLSIIARVLSAAVSIYMLLCTLRVLVSWLPSLDLGRSGDLLAS
                     ATDPYLGFFSRFPVLRAGSFDFSPIAALAVLAVANNLLTTLAFTGSLSVGVALGMLVS
                     ALWSAVAFVFSFLSFCALARVIAYAARWNSLHPLWRVIDAIINPILYRVNRIVYRDRI
                     VNYLQGLLTGFAAFVLVRFAGGALVGLVVQLLRSLPF"
     gene            11640..11939
                     /locus_tag="PLG14_02895"
     CDS             11640..11939
                     /locus_tag="PLG14_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOX48107.1"
                     /translation="MLTSRQKSHLAGLAQTLEPQIQLGKGGPSAGLVSQLDRILADHE
                     LVKLRFVDFKESRQELATELASSTKSELVRVIGNVAVLFRRNPDPKKRKIDFGDL"
     assembly_gap    12219..12318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12319..14228)
                     /locus_tag="PLG14_02900"
     CDS             complement(<12319..14228)
                     /locus_tag="PLG14_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48108.1"
                     /translation="MASVLSPARLKAARRSFYVFNVFNSFSFVLLSGSFVTLFALRLG
                     ASASFVGLLNACAYANYFFLPLGKRLVKRHRIVEVFGWGWMLRYVSMLPLLAAPLLAA
                     AGLPGPGFGLILAGVALFNVFRGVGLIGNNPVLASLAEGRDSGAFLVNVQIVNNLAAM
                     GTSLLAAFLLGRMGGSGLYLLLIGLGIAVGMAGSALLLKTPEPEAFRPKESKGLLATA
                     REAFREPAFRVFIEVLVLVSFAAGMARSFLPVYAKEVYEQGDDLIMLYSLLGSLGSVA
                     MGLLTRLLVDRLGAKPLYVIFTAVSALSLAPAVLTPSLQGPVGIALFLGALHFLSSFG
                     LSGEENAGQTYFFALVPRERTLDLAVVYFLAYGAGGTLGAGAGGLVLEGLRGQGLGRA
                     DSYRVFFGGLLVLLLWALIRMGSLVRLGSASVRESLGVIFSLRDLRAFDLLARLDRSV
                     DPDEEVRLIREIGASASPLSQRELLGYLASPRFDTRLEALLALENLPSLEEESLAALA
                     ADVAKNPFTTAYLAARILGKRGGPEALAAVKPALRAEDYMLQGAAVVAVARLGDRASR
                     GEIESILVGTSIPRVRISAAYALEILGERDSVPALVSCLRREDPPAYVSDELVLSIAS
                     ILGLMDRFYPMYA"
BASE COUNT         2025 a         4838 c         5234 g         2104 t
ORIGIN      
        1 ccgccgcgca aggccagcct agcaccctgg cccgcgtctc cacaacgaag ccgcggaaaa
       61 agctagcgcc gcctaactat gcggcgcccg cgcgcggcgc ttcgccgccc ctcgcgcgga
      121 gcctggccgg gctcttccgc taccggttgg agagcatgag gtcgtagccg cccctcgctt
      181 cggccgcggt gcccttgacg gagaggatcc aggtcccgga gaggccgcgc ctctcgtagg
      241 ggccgacagg gaagctcgag gggccttccc cggctaggta ctcgctcctc caggccaagg
      301 ctccggaggg ctcgaagagc tcgacggagg ccccgccagc ctcaagctcg accctgcccg
      361 agacggagag ccttgcgtcc ggggcggcga gctccaggct ggtcgcggcg cctttttcgc
      421 cgtagaggct tccctggatg gcgctctgct tgaggggcga gcaggccgag agggcgagtc
      481 ccgcggcaag gatggcggcg acggaggagg ccttcgcggg gccgcggtcg cagatggaga
      541 ttcgcttgtc catggaccga atataccgcg gaacggcgct tggaaaccag tattggatta
      601 tggtttccga caggccttcg cccccgacgc cggatggaaa attcggccgg cgcggggctc
      661 gccttgcgcg ccccatccgc cttcgctata gttcgcccca tgattccggc gctctaccag
      721 gacgaggaga tcctcgtcct cgacaagccc gctggcctcg cctcccagcc gggggcgggc
      781 gtgggcctct ccctcgtcga ggccgtcgag cgcgactacg gcttcaggcc cttcctcgtc
      841 cacaggctcg acaaggagac cgccggctgc atcctcgtcg cgaagagctc ccaggccgcg
      901 gcccgctgga ccgccgacat cgcctcgcgc tcggtcggca agttctaccg ggccgtcgta
      961 tcgggctccc tcgaaggcag gcggggccgc atcgccgatc ccgtgccggt ccggggcgaa
     1021 cagaaggcgg gcgagacctt ctggcgcctc gtcggccgct tcggcgccgc gggtccgggc
     1081 ggcgcccttg gcggcgctcc ggacggcgga ccggaggccg cgggcgggaa ggcggcgccc
     1141 ttcgcgccgc ccgggggtta ctcctacctc gagctcgagc tcgggacggg aaggacccac
     1201 cagatccgcc tccacctcgc gggctcgggc gcgcccatcc tgggcgacga ccggcacggc
     1261 gacttcgccc tcaacaagcg gctcaggaag gagctcggcc tcaggcgcct cctcctcgtc
     1321 gcctggcgcc tggagcttcc gggagggctg agcgtccgcg cctcggttcc cgaccacctc
     1381 agggccttcc tcgcggcctt ccccgacgcg cccgacccgg aggcgccgtg accgacgccc
     1441 ggcccgtccc cgagctccgc cacggcgcga gctggctcac gggcgacgag gagctcatcc
     1501 ccgtgcccgg cttccacgag gactggatcg gctcgcaccg tgacctcgtc ggggaggcga
     1561 agaacgtctg cgacgtcgtc ctcgggctgg gctggatctc cgtgtcggtc ttcagcgaag
     1621 gctacgtcga gctcctcgtc ccgaaccgcc gcgaccccgt cctgcgcgag cggatcgggc
     1681 gcctcctgcg cggcaacggc ggccgctggc ggagggtcct cgtgatgtcg atggacgagg
     1741 agggctacgc gagcctcggc cccggggacc tcgaggtccc ggggcggctc gaggccctgc
     1801 tgtcgggggc tacttccgcc tgatcccgag gaccgtcagg tcgtcgtact gctcgtcctc
     1861 gcggatgccc gcgtagagga cgtactccgg gtgctcggga tcggggttgt cccgcttccg
     1921 cgagcagtag agccggtact ggtcgaagtg cttctccagg aaggcgtcca ccttgcggtc
     1981 ggcgaggacg acgtccgact cgcccgcgtc cgggtcgggg tacatgcggt agaccttctc
     2041 cacagcgatg agggcgacga cgcgctcctc cagggtctcc ccgcaggtcg agaagtcgaa
     2101 ggtgagcacc gcgtcgggat tggggtcgtg ggccttcacg aggcggaagg agcccttcgc
     2161 gtccaccgcc gcgaggatct cgcagagccg gtcgtagccg aactcctcgt tgtcctggcc
     2221 gcccgagtgg tggctgtcgt ggggcttgtc cttctccgcg tcggcgcagg gcacgatctc
     2281 cagcttctcg ttccggaagt ggcgcttggc ctcctcgatg ccgtccgtgt agaggaggag
     2341 gatgtcgtcc ttctcgagct tgacgggcac ctggcggtag ggcgtcttca tctcgacgag
     2401 catgttgggg aaggtgcccg cggtcggggc gtcgggcagc tcgatgatct ccatccgccg
     2461 cgctgcgtcg cgccagacgt ggcagagccg gtcgcccgcg tggcagagcc gcgcgacgcc
     2521 ggtctcggcg tcgtagatcc cgagggtcag ggcggcgaag cgccccttgt agccgcggcc
     2581 ctcgatgaag ccgttgatct ggtaggccag ggactcgagc tcggggccct tcttcgcgac
     2641 gtcgcggccg tcgaagtggt tgatgaccat cgtcgcgacc tcgaccatga tgagggccgc
     2701 cgcgacgccc ttgcccgcga tgtcgcactt cacgaagtag tagtagcgcg agtccaggcg
     2761 gttgaagtcg aagtagtcgc cggagacgcc cttcgcgccc tcgtagtagc cgaagaactc
     2821 gaaggccttc tccgagaggc tgcccgtcgt gagcttggag cccttcggcc cgacctccag
     2881 ggggatgaac atcttctgga tgtcctttcc gagcgtgagg tccttggagg ccttggccgc
     2941 cttggccagg cccgaggcca tctgccccac cgcgtcggcg aggacgaaga tctcgtccct
     3001 gctcttcgag cccttgaagc ccttgagctc ctcgatgtcg cccagcttct ccttgtcctt
     3061 ctcgtcgcgg atcctcgtga tggccttctc cagcttcttg atcgggccca cgatgatgga
     3121 ggacaggagg atcgcgcccg cgacgccgat cgccagggcg accgccgcga tgatcagcgt
     3181 tatgcgaatg agttcggccg tggccttccg gacgtcggcg gcgatgatgt cggtcgtgac
     3241 ctccagcctg accatgccgc ggtagtagag ctggtcggag ccgcggcggt agaggatggg
     3301 cttgtagaag aggaagcggc cgccgccgcg cagggccgtc tcggggtcga aggcggggat
     3361 ggagccgacg gaggcgttcg ccagggcgaa gagcttcgcg ttgagctccc gctccaggcc
     3421 gcggctcgtg tcggcgatct cggcgagccg gcgctgggag gccgcgtcca gcttcgtggc
     3481 gagggcgcgg ccctcctgcg tgtaggcggc cagggtctcg ttcagcgacc cgacctcaag
     3541 ggccgcccgg tcgttgatct ccttcgcgat gccggccagg gagggcgtga gggcgtcgga
     3601 gagcctcgag gacccttgct tgagctccgc gccgtcgatc ttgccgccga tgcccgggtc
     3661 gttcgaggcc cacaccacgt ccgggtcggt ggaggcctcg gcgccgtagc ccgtgagggt
     3721 caggtaggcc gcgtcctcca tggccttggc ctgctggggc aggaagccga gctccaggac
     3781 gttctgggcg ggcaggtagg agcggccgcc ctgcgcgacg ctctccagga ggacgaggct
     3841 ccgctgctcc aggcccctcg cgaggttcgt cgactcggtg cggatcatga aggcgccgag
     3901 ggagaccgag aggaggagga cgacgaagat cacgaggagg gcgatgatga gggtgaactt
     3961 cgcgcgcagg ccggtcccgc gccgcctgag ggcgcgggcg acctgggcgg tggagccaga
     4021 gggcatggga cctcctgaga cgagggcgag gacctcgcgc ctgaccgcct cgccgtccag
     4081 gacgacggcg cggacctgcg cgagggagag ggctatgccg aggccggcga agaggacggc
     4141 cgcggcgacg atggcgtcgt agatcgagaa gcggtagcgg cgccgctcca ggatgcgcca
     4201 ggaggggacg tactcgcccc ccgaggcgcc gaacttgagg gtgcccgagg cgtcgacgga
     4261 gaggagggcg ccggaccagt accagccccg cttggggtgg aggacgccga cccggtaggt
     4321 ccccgcctcc aggccctcga aggccaggcc gtcgatctcc cggtcggagg cgacgcggaa
     4381 gccgccgtct gagcgctcgc ggacgaggtc gtagggctcc ctgccgtccc tgtccaggac
     4441 gaccgtcgcc gcgaggccct cggcggcgaa gccgcggccg aggaggcgga ggctcgtgcg
     4501 gccgaggtcg tcgcgccgcg cgtcgacgta gccgacggtc gtgtagggca cgtacttgtc
     4561 ggcgcgcagg aggatggtcg cggcgtcgcc gatgttgccc gtcctgtcca cggaggcgac
     4621 ggagaagagg tagtagccgt cgtcgacgtt cgcgtaggag gcgcggggct cggcgcccag
     4681 gagggcgggc ggcggcacgg gaggcccggc ccgctcgacg aggagggcct cgtagggcga
     4741 gaggccgggg aggggcggcc gggccgcggc ccgcgggccg gccggcaggg ccgccgccga
     4801 gcgctccagg ccgccgacgt agcggagctc ccaggtgtag ccggcgacgt cgtcggcctc
     4861 cgggggcgcc cagcgcacgg cgaagctgtt ggaggaaagg aagccgccct cgtcctgggg
     4921 cgggggctgg aggaggggcg gcggaggcgg cgtccggtcg cggtcgaagc ggagcctggc
     4981 cgccctggac cagttgcccg cgtagtcgac ggccgcgacg gcgagccacc aggcgccgtc
     5041 ctcgggcgcg ctgagcgaaa tccgggcctg gtcgggcagg gccatgagct tccgtggcgg
     5101 ctcggcggcc tggtcgcggc tccagaggta ggagtagccc tcgacgccgg aggagtcggg
     5161 ggcgagctgg acccgcgcgc tcgcggtctc ccgcctcgtc gcgacgcccg ggcgccagtc
     5221 cagggcctcg agggccggcg gggccacgct cgtgtcgggc tcgagggcga ggatgcgatc
     5281 ccgcctggcc tccgtgctct gccagaagac gtagggcttg ccgtccgaga cggcgatccg
     5341 tccgaaggag gcgtcgcccc ggctcccgga gagctccgac tcgggccagt cgaggccctt
     5401 gcgcgcgacc aggtagacgc ggttgccgcc gcggcggttg tcgaaccaga cgacggcggg
     5461 cctgccgtcg agctccagga cctggggcgc cgagcaggag cccgcgcccg aggagacccg
     5521 gtccgccgtc gcgctttggg cggcgcccga ggggtccagg gtcatcgcgt agacctgcgg
     5581 ctggcccagg agggtcgccc gctcccaggc gagccagagc ttccccgcgg cctcggcgag
     5641 gaaggggcgc tggttgtcga agttgtcggc ctcgttccgc tcgcggacca cggggtcggg
     5701 gaagtcggtg accctgcggg cggccgacca ggtcgcgccg ccgtcgcgcg agaccttgga
     5761 gaagagctgg aagctcctcc tcgctccggg caggagggac tggaagacga ccacgtcgac
     5821 ggggccgccc gagtcccggc tcctggcggc ggtcggaagg aagttgaggg ccaggccgtc
     5881 cccggcgccg acgaagggcg cgaagggcga ccaggaggag ccgtcccggc tcgtcgcgta
     5941 gaagagggag agggactcgg cctcgccctg ggtggcgaag aggagccagc cgcccgaggc
     6001 ggaggggaag acgcgggggg cgacggcggc cgcgctcggg gccggcctct ccgtccgctc
     6061 gaagcttacg cccccgtcgc gggagaggag gacctcgatg gcgccgtcgc cggcggcggc
     6121 ggctatcgcg acctgtccgt cctcggagga cgccgagtag aggaggggct cggagccgcg
     6181 gaactcgtag gggccggcga agcggcgcct cgtctcccag gagccgccgg agagccgggc
     6241 cagggagagc caggcccggc ccgcgtcgcc gccgggcacg ggctccgact cctgccagac
     6301 gacgaggacg ccgcccgagg aggagagggc cgtcgggaag cgccccgagc cctcgaccag
     6361 ggcgcgcggc gtctcccagt agtagtcctg ggccgtcgcc cgagccgcga cgccaaggag
     6421 gccgaggagg aggatcaggg ctctcgggaa ccttcggggc gcgcgctgca tggtaaaagc
     6481 tcttcctcgc tggcttccgc gcattatagc cgcgcctgga ttttcttgta caggtcgagg
     6541 agcttctgcg acttcttgtt cttggggtcc tggaggaggc gctcgacggt cgccagggcc
     6601 tggaggtagt tgccgccctg gaaggcctcg acggcggcgc ggtactgctc ctccgcgtag
     6661 ctcgagagga cgatctgcgc cgtgccgccc tggaaggtga ggatcctgtc cttgagcgcc
     6721 acggccgtct cgttgttcgg gtcgaggagg atggcctcgt tgagctgctc gagggcgaag
     6781 gtgaagcggc ccgtgtcgcc cgagtcgacg acccgccggg cggccgcgac gagctcggcg
     6841 gcgcggcgcg ccttggccgg gtcgggcggg ggcaggcgga tgccgaggag gatctccgcg
     6901 cggtccaggg cggccttgag gccggggtac ttcgggtcta tggcggccaa gtcctggagc
     6961 tcggagtagg cctcccgcgg ctcggtgtcc agtcggttcc gcgcctggac gaatttcgcg
     7021 ccgaagccgg agcggaaggc ctcggggtcg gtgagctggt cgatcttgag ggcgaggacg
     7081 ccggcctcct ggttcagggg gaagacgacc ttcacctcgt tgattttctg cttggagagg
     7141 gcgaagtact ggagggcgtc ggttttctgc cgcctgtcga ggaggctctt cccctcctcg
     7201 tagtagcgct tcgcgaggga gaggagctgg ctcatttcgg ggtagagcgg ggccgtcgcg
     7261 gggatgtcgc ggcccgtctt cacggagagg gcggcctgga cgagcttgag ccagtactcg
     7321 acctcgggct cgggctcgtc gccgtgggtc gtcttccagc gcgccctggc cctgaggagg
     7381 gagtcctccg agcggtcgaa gacgccctgg aagtagaagt tcttgccctc ggtgatgagg
     7441 cggcgcgtgt cgcggatgac ccggtcgttc tccgccttca cgatcgactc gcccaggagc
     7501 tggaggctcc ggtcggtctc ggccctaagg gccgcgttgt cctccagggc gaaggacgcg
     7561 aggtacttct cccgcgcctt cgtcaggcgg tcgcgcgcgg tctcgtagtc ctcggccgcg
     7621 agggcgcgcc tggcctcggc ggcgcggcgc tccgcttcca ggcgggccga gtcggcggcg
     7681 cgcttctggt ccagggccct ggccgcgacc gcgagccgct ccgtctcgag ggcgacggcc
     7741 tcgcggtcca gaccccgggc cttctccgcc cagagctgga cggaaggggc gcccgcgacg
     7801 tagggcgtct cgcgctccag gcgccggagg tactcggcca ggcggctccg cagggccttg
     7861 aggctcgcct cctcggcgcc caggagctcg gcgctccggc ccgggtagcg gaggaggacc
     7921 tcggcggcgc cctcctgggg caggccctcg aggaggcggc ggccctcggc gacggcggcc
     7981 ttccgcgagg cgaggtctgc ctcgaagcgg ccgtactcga ggccggaggc gcgggcggct
     8041 atggagacct cgtagccgcg gagggtgtcg acggcggccg cgaggcggcc gtcgtagctg
     8101 ccctggaccc gcgccgcccg gtcgtcgccg tagcccgccg ccgtccagcg cgcgagctct
     8161 tcggcgaggg ccctcgcggg ctcgcgctcg gcggagaggc catcgccggc cgcggcgatc
     8221 tccttccgcc tcgccgcgaa gcccgcgagg gtcggctcca cgggggcgtc gtccgggagg
     8281 ctctccgcga agcggcgggc ctcctcgtcg taggccgccg cccttccggc gatggcggcg
     8341 atgcgggcct gggccgcggc cgccttgccg aggtggcgga tccgctcgta ggccgcggcc
     8401 ttgcccgcca ggacggccct gccgtactgg gtgggctcgg ggaggatctc cgtgggcgcg
     8461 accaggctcc agaggccgag ggcccgcagg cccggctccg ccagggccgc cgtccgctcg
     8521 aagccggtct tcgcctcttc ccagcggccc gcgtcgtagg cggcctccgc ggcggcggcg
     8581 tactcgtcga tcctgccgtc cagcgcggcc tgcatgccgt tgaggagggc tatccactgg
     8641 ttgtccatcg ctcccgagat tccctcgggg agggcggccg tcgtgcggcc cagggtgaag
     8701 cggaaggcga agggcaggaa ggaggagtcc gtcgccgtcc ggtccgcggc ctggatcacc
     8761 tcgaactggc gctgcatgga gcggccggcc gcgaccacgg cgttccgcct ccgcgcccgc
     8821 tcgaggaggg cggcctcggc cgcgggccag gccgactccg cggccgcggg atcccccgcg
     8881 gcgaaggcgg ccctgagccg ctcgacgacg gggacgaggg aggcctgcga cgaggcgtag
     8941 acgacgatct cggtcttcac cctgtccacg aggcccgcga cggactgttt cgtgagctgg
     9001 tcgtaaggcc cccggtcgaa ctcgtcccgg tagaggacga agccctcctc gtaggccctg
     9061 gccgcgtcga cgtagcgccc cgcctcgatg agggtccgtc ccttggccat gatggcgtcg
     9121 aactgggccc ggttgtagac gaagagggag gcgagcttcg tcctctcgat gaaggccttc
     9181 gtgccggggt cgttggggtc gatgctctgg agccgtgcga tggcgtcgag cttggccttc
     9241 tcgtccaggg ggttgttgac gagggtgtcg atgaggtcct tgcccgcctg gttgtagctc
     9301 tccctgagct cgatgacctt cctgagcttg gcctgggcgt cgtcgaagcg gtcggggtac
     9361 ttcttgatga actcggtgag gtagaggatg gcctcgttgt agcgctggcc ggcgatgagc
     9421 gtgtccgcct cgcgcagggg atcctccttc ttgcccatgc cgaaggccgc cgggactccg
     9481 aggacggtcc aggcggccag gatgaggagg aggaggcgcg aggcccgcgc cctagggctg
     9541 cgggacgggg ggacgctcat cgggccgatg ttccatcacc tttccaggac tagaatatac
     9601 ctcggcgccc ttggccggac ggggaggtgc ccgctttatt ctcggcggcc gcggaccttg
     9661 attgtaggga gaaaggccga gtatagaaat cccgcggaag tgagggtata attcctcttg
     9721 agatgaagcg aagccgcctc ctgcctctcc tcctcctcgc gctcgtcccc gcggccaggg
     9781 gcgcggccca gctcgctccc tccgtctacc aggccataag cgagtacttc aaccccgatc
     9841 cgaacgcggg cctcaccgcc ttccgctccc tcctcatccc catgggaggc ctcgccgagg
     9901 gcatgggcat ggcctacacg gccgtcgcca aggactcttc ctacttcgag tccaatcccg
     9961 ccgcctcgag cggcctgggc cagaccgagc tctcggtctt ccacaacaac tggatagccg
    10021 acacgcgcat cgagggggcg gtctacacga tccgctacaa gaacctgggc ttcggcgccg
    10081 gcgggaagtg gctctacctg cccttcaccg cctacgacaa gtacggcgac cgcgagggga
    10141 ccggctacta ctcggaggcg accgcggcct tcaacgtatc ctacaatttc ctggcgggct
    10201 actacttcaa cggcctggcc ctgggcgcga cgggcaaggc ttcctaccgc tccgtgcccg
    10261 cggccatagc ggaggccgcg ggcaactcca cggctgccgt catggtcgac ctgggcgccc
    10321 tctcgcgctt caacctcctc aagttctaca actcccggga aaagaacttc tccgtaggcc
    10381 tggccctcaa gaacctcggc cctccctccc agggcgagcc cctgccgacc gtcgcgagct
    10441 tcggcctcgc ctattcgccc ctcaggcccc tccttttctc cctggacgtg tccaagccga
    10501 tcaacctggt agagatcgcc aagtcagagc ggccgagcta cggcgccggc ttcgaagtca
    10561 ggatgaccga cttcttcggc ctccacggcg gcttcctcct caagggcggc aatccccgcc
    10621 tctccgtcgg ctcctccttc gacatcgagc tcgtgaaggt cgtcgtcaac tacaccctgg
    10681 acctgacgac ccagctcacg cccctgaacc gcatctctgt ccaggcctcc ttctccctgg
    10741 gcgacctggg gcgggcggag cttgcgaaaa aggtggaaaa tctatatctt aaggggctgg
    10801 aagcctacgc cggaggcgat tccgccgccg ccatggcggc ctggaccgag gtcctgaagc
    10861 tcgattccgg cttcgacccg gccagggaga gcctccgcgc ggcccagggc gccaccgatc
    10921 tccagaagac tatcgaagag ctgcagaagc tcgattgatg aaaggaaacc ttccgtggtc
    10981 ctgtcgataa tcgcccgcgt cctctcggcg gccgtttcca tctatatgct cctctgcacg
    11041 ctccgcgtcc tcgtctcctg gctcccgagc ctggacctgg gacggagcgg cgacctcctc
    11101 gcctccgcga ccgaccccta cctcggcttc ttctcgcgct tcccggtcct ccgcgccggc
    11161 agcttcgatt tctcccccat cgcggccctc gccgtcctgg ccgtcgcgaa caacctcctc
    11221 acgaccctcg ccttcaccgg cagcctctcc gtcggagtcg ccctgggcat gctggtctcc
    11281 gccctctggt cggccgtcgc cttcgtcttc tccttcctct ccttctgcgc cctggcccgc
    11341 gtcatcgcct acgccgcccg ctggaacagc ctccacccgc tgtggagggt gatcgacgcg
    11401 atcatcaacc cgatcctcta ccgcgtgaac cggatcgtct accgcgaccg catcgtgaac
    11461 tatctccagg gcctcctgac cggcttcgcc gccttcgtcc tcgtccgctt cgccggcgga
    11521 gccctggtcg gactcgtggt ccagcttctc cgaagccttc ccttctagcg gaagagcagc
    11581 ctccgaccct cggcgccctt gcggggaacc gcgccgaaca gcgatcataa ggatacgcca
    11641 tgcttacttc caggcagaag agccacctcg cggggctcgc ccagaccctc gaaccccaga
    11701 tccagctcgg gaagggcggc cccagcgccg gcctcgtctc ccagctcgac cggatcctcg
    11761 cggaccacga gctcgtcaag ctgcgcttcg tcgacttcaa ggagtcgcgg caggagctcg
    11821 cgaccgagct cgcgtcgagc acgaagagcg agctcgtccg cgtgatcggc aacgtcgccg
    11881 tcctcttccg gcgcaatccc gaccccaaga agcgcaagat cgacttcggc gacctctagg
    11941 tccgcccgcg cgggccgccg cccgcggcca tgaacggagc gcccctccat ccgcatgccg
    12001 gacggagggg cgcttttttc cgcgccgcgc cgcgcgggcc gtcaggccag ggcgacgtag
    12061 gcggcgagga agaagcggaa gccccggtag ccgagcttcg ggtccagggc ggcctcggcc
    12121 aggactatct ccacgccggg gtcggctccg aggcccatgg ccatgcgggc gatgggctcg
    12181 ccccggggcg ggtcggccag gatgccctcc gcggccttnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnngc gtacatcggg tagaagcggt ccatgaggcc gagtatcgac
    12361 gcgatggaga ggacaagctc gtcgctcacg taggcgggcg gatcctcgcg ccggagacag
    12421 gagacgaggg ccgggacgga gtccctctcg cccaggatct cgagggcgta ggccgcggag
    12481 atcctgaccc gcggaatgga ggtccctacg aggatggatt cgatctcccc cctggacgcg
    12541 cggtcgccga ggcgcgcgac ggccacgacg gccgcgccct ggagcatgta gtcctccgcg
    12601 cgcagggccg gcttgaccgc ggccagggcc tcaggcccgc cgcgctttcc gaggatgcgc
    12661 gcggccaggt aggccgtcgt gaaggggttc ttcgcgacgt cggccgctaa ggccgcgagg
    12721 gactcctcct ccagggaggg caggttctcc agggcgagga gggcctcgag cctcgtgtcg
    12781 aagcggggcg aggcgaggta gcccaggagc tcccgctggc tcaaggggga ggccgaggcg
    12841 ccgatctcgc ggatgagccg cacctcctcg tcggggtcga cgctccggtc caggcgggcg
    12901 aggaggtcga aggcgcggag gtcgcggagc gagaagatga cgcccaggga ctcgcgcacc
    12961 gaggcggagc cgagcctgac gaggcttccc atgcggatga gggcccacag cagcaggacg
    13021 aggaggccgc cgaagaagac gcggtaggag tcggcccggc ccaggccctg cccgcggagg
    13081 ccctccagga cgaggccgcc cgcgccggcg cccagggtgc cgcccgcgcc gtaggccagg
    13141 aaatagacga cggcgaggtc cagggtcctc tccctcggca cgagggcgaa gaagtaggtc
    13201 tggccggcgt tctcctcgcc cgagaggccg aaggaggaga ggaagtggag ggcccccagg
    13261 aagagggcga tcccgacggg gccttggagg gagggcgtga ggacggccgg cgcgaggctc
    13321 aaggccgaga cggcggtgaa gatgacgtag aggggcttgg cgccgagcct gtccacgagg
    13381 agccgcgtga ggaggcccat ggccaccgat cccaggcttc cgaggaggga gtagagcatg
    13441 atgaggtcgt cgccctgttc gtagacctcc ttggcgtaga cggggaggaa ggagcgggcc
    13501 atgcccgccg cgaaggagac gaggaccagg acctcgatga agacgcggaa ggcgggctcc
    13561 cggaaggcct cccgcgccgt cgcgaggaga cccttcgact ccttgggcct gaaggcctcg
    13621 ggctcgggcg tcttgaggag gagggcggag ccggccatgc cgaccgcgat gcccaggccg
    13681 atgaggagga ggtagaggcc cgagcccccc atccgtccga ggaggaaggc cgcgaggagg
    13741 ctcgtcccca tggccgcgag gttgttgacg atctgcacgt tgacgaggaa ggcgccggag
    13801 tccctgccct ccgccaggga ggcgaggacg ggattgttgc cgatgaggcc gacgccgcgg
    13861 aagacgttga agagggccac ccccgcgagg atgaggccga agcccgggcc aggcaggccc
    13921 gcggcggcga ggaggggggc ggcgaggagg gggagcatcg agacgtagcg gagcatccag
    13981 ccccagccga agacctcgac gatgcggtgc cgcttgacca ggcgcttccc gagggggagg
    14041 aagaagtagt tcgcgtaggc gcaggcgttg aggagcccca cgaaggaggc ggaggcgccg
    14101 agcctgagcg cgaagagggt gacgaagctg cccgagagca ggacgaagga gaaggagttg
    14161 aagacgttga agacgtagaa ggatctgcgc gcggccttga gcctggcggg cgagaggacc
    14221 gaagccatat ccgcccagta taggggagaa gccctcccgc cgccagtgct actcggaggc
    14281 ggggccgcgc ggcgccgcgg c
//
LOCUS       DAOOSJ010000032        14226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2870_length_14226_cov_7.420154, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000032 DAOOSJ010000000
VERSION     DAOOSJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_14226_cov_7.420154"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLG14_02905"
     CDS             complement(<1..1150)
                     /locus_tag="PLG14_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOX48109.1"
                     /translation="MIDLKIIEAQRKKNIERCREKGIILPTYAQMRDPAKIPQSVKDE
                     LKGVGLWDVHSRNLFRVNWHNEAKTKGGGFAGVNFIELPRSVTGTKARIVGLSGKWFP
                     TGAHKVGAALSCLLPELVTGRFDPTTKKAVWPSTGNYCRGGAYISRLLSCPSIAILPA
                     GMSKERFAWLKTMAEEVIATPGCESNVKEIFDKCVELERTRPDAVIFNQFDQLPNHLW
                     HYAVTGPAMEEVFNKIKGPSSHVAGAVLSSGSAGTLGSGSYLRETFPGARVAVAEALQ
                     CPTILENGFGDHRIEGIGDKHVPWIHNLRETDAAIGVDDELPMRFIRLFNEPAGRAVL
                     AENGVPQDVLDRLDWLGISGVGNMLGAVKFAKYYELGEDDVVFTMFTDS"
     gene            complement(1243..2403)
                     /locus_tag="PLG14_02910"
     CDS             complement(1243..2403)
                     /locus_tag="PLG14_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOX48110.1"
                     /translation="MRWLYRCPSCGAEYAIEPGRYLCDRCSSRNAPGMPLGGVLECAW
                     EGGSPEPGSVPLPVGREWLPPLPVGDTPLWAPARLRAELGFPGLWLKDDTCEPSGSYK
                     DRASYLVAGFAKAHGLNEIALASTGNAASSMACVGAAAGIKVTVFVPKAAPVAKRIQV
                     LQYGAELREVDGTYDLAFDQSLDYSAKTGVLSRNTAYNPLTIEGKKTASFEILRDLRR
                     ATGDRKAAPDHVFVPTGDGVIVAGVIRGFEDLVRMGELERMPTVWAAQAEGSSAIARA
                     LESGTWDFRPSDTIADSISVDAPRNGYYALEKLKAHGGRGVVVSDAEILEAQRLGSSR
                     AGLFAEPSSACALAGFLKARGEIGKGETAVVMLTGSGLKDVKSAARAVGAAY"
     gene            complement(2400..3608)
                     /locus_tag="PLG14_02915"
     CDS             complement(2400..3608)
                     /locus_tag="PLG14_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOX48111.1"
                     /translation="MDQQKILEKAKAYRDYTAQNLSKMIQVPGFSCTEKDRVLLLKKM
                     CEEAGMEDLRIDGLGSLLGRVGKGGKKLVFDAHIDTVGVGDPAQWKLPPHSGIVKDGL
                     VHGRGASDQLGGAASMITAARILKEMAYSGDYEVWFAFTTIEEDCDGLCWKYLIEEEK
                     FVPDFVVSTEPTSCRLYRGHRGRMEIQIEIQGISCHGSAPERGESAAYKAARAALAIE
                     KLNAELEPDDDKFLGKGTITVSQIKVHGPSQCAVPDQAMLYCDRRLTWGEDDKLAISQ
                     VEKALKAAGVDKFKVFMPEYSQPAYTGLLYKQELYFPTWKIPNDHFLVRSGVDAYTKL
                     YAAAPVVDKWTFSTNLVAVCGRHKIPSIGFGPGDESQAHAPNEINRVDDLEKCAAFYA
                     ALPFAMEARA"
     gene            complement(3610..4944)
                     /gene="ssnA"
                     /locus_tag="PLG14_02920"
     CDS             complement(3610..4944)
                     /gene="ssnA"
                     /locus_tag="PLG14_02920"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOX48112.1"
                     /translation="MTIFESVRVISFEPPSVSEPLDVAVAGPGESGAGTVVEVGKGLA
                     AKRPGAKRVQGAYLSPGLVLGHTHLYSALARGLMVGIEPSKDFAQQLGHLWWRLDRAI
                     DLPILEASGLAGCADAAMCGVTSVVDHHAGPEAIEGSLSVLRKAYEEIGIRGLLCYET
                     TDRNGAEGGRAGVAENVRFAREIDADRKAGREPLVDGLIGGHAGFTIGDETLGLMADA
                     VKSTGRGVHIHLGEDKYDAVDSRHRFGADLVERMERAGCLTRKSIVGHGVWLTRSEVE
                     LMNERGAYLAHNARSNMNNSVGYNGLLQDHAKVVLGTDGMSADMLEEFKFAAFRHRES
                     QGPWWPGDFLKCLDRGNRLMEDYLGGAFGKVEAGARADLVLWDYEPPTPLVGENLAGH
                     LAFGLSSRSVRSVMVNGKFVVEERKPAFDAARAGALGREQAIRLWKRMEERR"
     gene            complement(4941..8129)
                     /locus_tag="PLG14_02925"
     CDS             complement(4941..8129)
                     /locus_tag="PLG14_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48113.1"
                     /translation="MSKVMDVTPLPALLERIAGEYRAKGTIYEIPERAFRGVFELEAS
                     SPGIAVMGGRASLPVGPAAGPHSQIAPNLVAAYLAGSRAFELKTVQENDALEIEKPCI
                     LALDEGHNTEWSTELSLEAAREEYLRGWIAVNLLAALWSAKPRDFFFNMSVGYTLAGI
                     RGAKVDAYIEGMRSPEKTAFWGRAMRDLEAFVEGPSFAAAFGAEALGKARTLLSDFPA
                     KPVHSVTLSTMHGCPPDEIGRIGRYLIEDKGFDAYVKLNPTLLGYDEARRILDATGWT
                     DIGLKRATFEHDLQWKDALELVRSLGEAAEANGRRFGIKLSNTLANENDGGFLPGAER
                     YMSGRALFPVTTRLAARLAEALPGFPRRLSYCGGASALNARDLVEAGLGPLTVATDIL
                     KPGGYLRLVPMARAAAEALAANPAANRGEGRPDAKAIARLADAALERPEYRKEWKSGN
                     ASIKRALPLFDCFAAPCVEACPVNQKVPEYIRRAASGDAKAALETILADNPLPCVTGT
                     LCDHVCQAACSRNDYEGSVEIRAVKLACAKAASLPPGKPAKAAFPGKVAVVGAGPAGL
                     ACAHHLALSGAKVVVLDEAASAGGVPANVIPRFRISREDLAKDIGRIEALGAEFRFGF
                     RAKDLASLKAEGFSAVFLAAGAPAARELRLEGEGVRVVDALAFLEATSAGPGEFAGYR
                     RVVVAGGGNTAMDAVRAATRLPSVESVRLSYRRTLAEMPADREELENAMREAAVLDGA
                     AGAGSSGSGAAGAAHGQGALLELSLPERAAPGKLTLRKMRLGEKDASGRRSPLPSDET
                     IELDCDLLVAAVGETPDPALMKAFGIPVGKDGRPVYDPATQETPVAGLYVGGDAARGP
                     ASIIAAEADGRRAAYAILRKAGVEPPLAPYSPPEPRAERLASRGEFAHPLAAGSEGFA
                     AREAERCLECDSACLRCVEVCPNRANMAIPRPSGSGAQAFQILHVDYLCNECGNCGLF
                     CPYEGEPFRGKATLFADRASLEASKNAGFALLQGGAVALRAAYEGPVAEFAQGASAPA
                     AGGGSSASDGAAMLALARAVARDHAYLSGGAK"
     gene            complement(8126..9526)
                     /locus_tag="PLG14_02930"
     CDS             complement(8126..9526)
                     /locus_tag="PLG14_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX48114.1"
                     /translation="MIVQNGLVALPGEEDFLRLDLRVRGGRIAEIAPRLEAEAGEETV
                     DASGLLVLPGAIDPHVHFDEPGFTHREDFRHGTAEAAKGGVTTVIDMPCTSLPPVTTL
                     AALEGKLAAVSPSALVDYAFYGGVSGHAAEASLGSGGSGGAMAELAPKVVGFKCYFIS
                     GMDSFTSVTHEAFARILAEGERLGRPILLHAEDLDYVTAATARAKALRGAAAPEWADY
                     AAGRPAAAELVACASALALAKGREKTLHVVHVGVPEAAELLHAGGASCETCAHYLAFT
                     DADFPRLGASLKTAPVVKGAAEREGLWRLLASGAVDFVASDHAPAPEEEKNTGNPWTA
                     YGGIPGTGTMLPYLLSEGLLSGRLSLPRFLDATGGAASRRYGLSARKGSIAVGKDADL
                     VLVDPSASTRIEGGKLLSKGKITPFEGMRLTGRILVTYVRGAPVYRAARGADEAEAGD
                     IVAQPGSGKFLTWGYR"
     gene            complement(9523..11820)
                     /locus_tag="PLG14_02935"
     CDS             complement(9523..11820)
                     /locus_tag="PLG14_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOX48115.1"
                     /translation="MKAIPEASLNVRGKTAYIDDLPEPRSLLHAAVKLSESARGRITR
                     IDPSAALALDPSVRVVTHADVPGENQLGFTVADEPLLPAHEWEYWGQPLVLCLARTKS
                     LARRACELVAVEGEPLEPVLDAREAAAKGEFILPPRTQASGDTAAAFASAAFAAEGRV
                     ESGGQEHVYLETQGALAEPLDGERFRIISGTQGPTVVQRAAARILGLPMSSVEVEARR
                     LGGGFGGKEDQATSWACLAALGARVSGRPVKLYLDRRTDMRATGKRHPYTSDFRIAMD
                     AEGRITAFEADYYQNSGCSCDLSAAIISRTLFHAVGAYRVPNVRVTGYLCRTNLPSFT
                     AFRGFGAPQGFFVFEAALDALAAKAGIDPVELRRKNLIAEGDTTHYGMPMSGVRAGES
                     FDRLLEKTGWSGLRAEIDAFNASHPLEKKGAAVLPNCFGVSFTKLPMNQGGALVHAYT
                     DGSVLVSTGAVEMGQQVSRKIARVVARTLGIPLERVAVERTTTLTVANTVPTAASTGS
                     DINGMAALVACEELLSRLLRKAEALVGEPASNLSIRDGRLMRGCEDAGLSWEQLVEKC
                     HEARIDLSAHGFYATPGLFYDMKAERGSPFAYHVYGAALVVVRLDVLRGDYEIERAAI
                     VHDVGRSLDPSVDLGQIEGAFAQGIGWSLLEELKFSEEGRLLSDTLSTYKLPDAHFLA
                     SGVDVEFLPEADNPRAVLNSKAIGEPPLVYGIAGYFALLDALRAARPDGPAMYDLPLT
                     PEKALDYLSGSRPEFLAKAGKGGKR"
     gene            complement(11817..13373)
                     /locus_tag="PLG14_02940"
     CDS             complement(11817..13373)
                     /locus_tag="PLG14_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOX48116.1"
                     /translation="MSRTLRFLANDLVVETGDWAGRPALDFIRGDLGLTGAKEGCREG
                     DCGACAVLVGEKPLAGGSGAAAVYKAVPSCILALGELEGRHLVTIEGLSAGAAAAGLE
                     KGLTPVMRAFLEENASQCGFCSPGFIISLTAWLLAGRRMDEEGALEAVEGNLCRCTGY
                     GSIRRAASRILKEFADLPSEPAARLAALAKAGVVPASLEAFADGRLLPAAPAGSDGAA
                     PAHAAASAPAAGAAPSGGALPAAGSPLPALILGGGTDFYVRNTDPEPREGFRLLDADP
                     RLKAIERRGSVLEVGAAVTVRDFFASPEVRAALPGVEAFEGRFASVLIRNRATLGGNV
                     VNASPVADMTSILVALGASLRLERAGQAPRELALERLYLGYKRLDLAPGEIVAAFLIP
                     VAGALRLSFEKASKRKSLDIAAVNTAAALELDGRRITRARISAGGVAATPLLLEATSD
                     LLAGREVSAATAVEAARSAVSEVSPIGDVRGSAGYRIRVLERLVLAHFVSLFPEDRAL
                     AEGVAKEVLK"
     gene            complement(13404..14099)
                     /locus_tag="PLG14_02945"
     CDS             complement(13404..14099)
                     /locus_tag="PLG14_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX48117.1"
                     /translation="MSISRERLSADVHQELLGQIIRGQFAPGQRLKDSELAERLGVSR
                     TPVREALLRLEREGFISSQQHMGFSVKRLEESEIKEVYPLVRILECSALKASPLPSPE
                     KLTLLRELDRSMKQEGADPLRRIELDSAWHKALVEDNGNRYLMRVLADSKRILFRYEY
                     AFMQVSELVSESMEEHEAILAALERGDRRGAVVLLGAHWERCTEETLVDFQATSGAGR
                     ECPASIESAQAKS"
BASE COUNT         1925 a         4673 c         5514 g         2114 t
ORIGIN      
        1 tggagtcggt gaacatcgtg aagacgacgt cgtcctcgcc gagctcgtag tacttcgcga
       61 acttgaccgc gcccagcatg ttgcccacgc cggagatgcc gagccagtcc aggcggtcca
      121 ggacgtcctg ggggacgccg ttctcggcga ggacggcgcg gcccgcgggc tcgttgaaga
      181 ggcggatgaa gcgcatcggg agctcgtcgt ccacgccgat ggccgcgtcg gtctcgcgga
      241 ggttgtggat ccagggcacg tgcttgtcgc cgatgccctc gatgcggtgg tcgccgaagc
      301 cgttctcgag gatggtcggg cactggaggg cttccgcgac ggcgacgcgg gcgccgggga
      361 aggtctcgcg gaggtaggag cccgagccca gggtgccggc ggagccggag gagaggacgg
      421 cgcccgcgac gtgcgaggac ggccccttga tcttgttgaa gacctcctcc atggccgggc
      481 cggtgacggc gtagtgccag aggtggttcg ggagctggtc gaactggttg aagatgacgg
      541 cgtccggcct cgtcctctcg agctcgacgc acttgtcgaa gatctccttg acgttggact
      601 cgcagcccgg ggtcgcgatg acctcctcgg ccatggtctt gagccaggcg aagcgctcct
      661 tcgacatgcc cgcggggagg atggcgatgg aggggcagga gaggaggcgg gagatgtagg
      721 cgccgccgcg gcagtagttg cccgtggagg gccagacggc cttcttggtc gtggggtcga
      781 agcggccggt cacgagctcg gggaggaggc aggagagggc cgcgccgacc ttgtgggcgc
      841 ccgtcgggaa ccacttgccg gagaggccga cgatgcgcgc cttcgtgccg gtgacggagc
      901 gggggagctc gatgaagttg acgccggcga agccgccgcc tttggtcttg gcctcgttgt
      961 gccagttgac gcggaagagg ttgcggctgt ggacgtccca gaggccgacg cccttgagct
     1021 cgtccttgac ggactggggg atcttcgcgg gatcccgcat ctgcgcgtag gtcggcagga
     1081 tgatgccctt ctccctgcag cgctcgatgt tcttcttcct ctgggcctcg attatcttca
     1141 ggtcgatcat gttgcgttct cctcgttcgc gcgtcgcgcg attgcttgtc tcgccgcccg
     1201 ggcgccccgc gacgcggggc ttccctcggg cggccttcgg tcttagtaag cggcccccac
     1261 ggccctcgcg gcgctcttca cgtccttgag gcccgagccc gtgagcatga cgacggccgt
     1321 ctcgcccttc cctatctccc cgcgcgcctt caggaagccc gcgagcgcgc aggcggagga
     1381 gggctcggcg aagagccccg ccctggagga gccgaggcgc tgggcctcga ggatctccgc
     1441 gtcggagact acgaccccgc ggccgccgtg cgccttcagc ttctcgaggg cgtagtagcc
     1501 gttcctcggg gcgtcgacgg agatggagtc ggcgatggtg tcggaggggc ggaagtccca
     1561 cgtccccgat tcgagggccc gcgcgatggc actcgagccc tcggcttggg cggcccagac
     1621 ggtcggcatc ctctcgagct cgcccatcct cacgaggtcc tcgaagccgc ggatcacgcc
     1681 ggcgacgatc acgccgtcgc ccgtcgggac gaagacgtgg tcgggcgccg ccttccggtc
     1741 gcccgtcgcg cggcggaggt cgcggaggat ctcgaaggag gcggtcttct tcccctcgat
     1801 ggtgaggggg ttgtaggccg tgttacggga gaggacgccg gtcttggcgc tgtagtcgag
     1861 cgactggtcg aaggcgaggt cgtaggtgcc gtccacctcg cggagctcgg cgccgtactg
     1921 gagcacctgg atccgcttgg cgacgggcgc ggccttgggc acgaagacgg tcaccttgat
     1981 cccggcggcc gcgccgacgc aggccatgct ggaggccgcg ttgcccgtgg aggccagggc
     2041 gatctcattg aggccgtgcg ccttggcgaa gcccgcgacg aggtagctcg ccctgtcctt
     2101 gtaggagccc gagggctcgc aggtgtcgtc cttgagccag aggccgggga agcccagctc
     2161 cgccctgagg cgcgcgggcg cccagagggg cgtgtcgccg acggggaggg gcggaagcca
     2221 ctcgcgcccg acggggaggg ggaccgagcc gggctcgggg ctccctccct cccaggcgca
     2281 ctcgaggacg ccgccgagcg gcatgcccgg cgcgttgcgc gaggagcacc ggtcgcagag
     2341 gtagcggccg ggctcgatcg cgtactcggc gccgcaggac gggcaccggt agagccatct
     2401 catgcccgcg cctccatggc gaagggcagg gccgcgtaga aggcggcgca cttctcgagg
     2461 tcgtcgaccc ggttgatctc gttcggggcg tgggcctggg actcgtcgcc ggggccgaaa
     2521 ccgatggagg ggatcttgtg gcggccgcag accgccacga ggttcgtgga gaaggtccac
     2581 ttgtcgacga cgggggcggc ggcgtagagc ttcgtgtagg cgtccacgcc ggagcggacc
     2641 aggaagtggt cgttcgggat cttccaggtg gggaagtaga gctcctgctt gtagaggagg
     2701 ccggtgtagg ccggctggga gtactcgggc atgaagacct tgaacttgtc gacgccggcg
     2761 gccttgaggg ccttctcgac ctgggagatg gcgagcttgt cgtcctctcc ccaggtgagg
     2821 cgcctgtcgc agtagagcat ggcctggtcc ggtaccgcgc actgcgaggg gccgtggacc
     2881 ttgatctggg agacggtgat cgtgcccttg cccaggaact tgtcgtcgtc cggctctagc
     2941 tcggcgttga gcttctcgat cgcgagggcg gcgcgcgcgg ccttgtaggc ggcgctctcc
     3001 ccgcgctcgg gggcggagcc gtggcaggag atcccttgga tctcgatctg gatctccatg
     3061 cggccccggt gcccgcggta gagccggcag ctcgtgggct cggtggagac gacgaagtcg
     3121 gggacgaact tttcctcctc gatgaggtac ttccagcaga ggccgtcgca gtcctcctcg
     3181 atcgtcgtga aggcgaacca gacctcgtag tcgcccgagt aggccatctc cttgaggatg
     3241 cgcgcggcgg tgatcatgga ggccgcgccg ccaagctggt cggaggcgcc gcggccgtgg
     3301 acgagtccgt ccttgacgat ccccgagtgc ggggggagct tccactgggc ggggtccccg
     3361 acgccgaccg tgtcgatgtg cgcgtcgaag acgagcttct tcccgccttt gcccacgcgg
     3421 ccgaggaggg agccgagccc gtcgatccgg aggtcctcca tgccggcctc ctcgcacatc
     3481 ttcttgagga ggaggacgcg atccttctcg gtgcagctga agccgggcac ctgtatcatc
     3541 ttgctgaggt tctgggcggt gtagtcgcgg taggccttcg ccttctcgag gatcttctgc
     3601 tggtccatcc tatctccttt cttccatgcg cttccaaaga cgtatcgcct gctcgcggcc
     3661 cagggccccg gctcgggcgg cgtcgaaggc gggcttccgc tcctcgacga cgaattttcc
     3721 gttcaccatg accgaccgga cggaccgcga ggagaggccg aaggccaagt ggcccgcgag
     3781 gttctccccg acgaggggcg tcggcggctc gtagtcccag agcacgaggt cggcgcgcgc
     3841 gcccgcctcc accttgccga aggcgccgcc caggtagtcc tccatgaggc ggttgccgcg
     3901 gtccaggcac ttgaggaagt cgccgggcca ccaggggccc tggctctcgc ggtgccggaa
     3961 ggcggcgaac ttgaactcct ccagcatgtc ggcgctcatg ccgtccgtcc cgagcacgac
     4021 cttggcgtgg tcctggagga ggccgttgta gccgaccgag ttgttcatgt tggagcgcgc
     4081 gttgtgcgcc aggtaggcgc cgcgctcgtt catgagctcg acctcggagc gggtgagcca
     4141 gacgccgtgg cccacgatcg acttccgcgt gaggcagccc gcgcgctcca tgcgctccac
     4201 gaggtcggcg ccgaagcggt ggcgggagtc gaccgcgtcg tacttgtcct ccccgaggtg
     4261 gatgtggacg ccgcggcccg tggacttgac ggcgtcggcc atcaggccca gggtctcgtc
     4321 gccgatcgtg aaaccggcgt gcccgccgat gaggccgtcc acgaggggct ctcggccggc
     4381 cttccggtcg gcgtcgatct cgcgggcgaa gcggacgttc tccgcgacgc cggcgcgccc
     4441 gccctctgcc ccgttgcggt ccgtggtctc gtagcagagg aggccgcgga tcccgatctc
     4501 ctcgtaggcc ttgcggagga cggagaggct cccctcgatc gcctcggggc cggcgtggtg
     4561 gtcgaccacc gaggtgacgc cgcacatcgc ggcgtccgcg cagcccgcga ggccgctcgc
     4621 ctccaggatg ggcaggtcga tcgcgcggtc caggcgccac cagaggtgtc cgagctgctg
     4681 cgcgaagtcc ttggagggct cgatgccgac catgaggccg cgggccaggg ccgagtagag
     4741 gtgggtatgg cccaggacca ggccggggga gaggtaggct ccctggactc gcttggcccc
     4801 ggggcgtttc gcggcgaggc ccttgccgac ctcgacgacg gtgcccgccc cgctctcgcc
     4861 cgggccggcg accgcgacgt cgagaggctc ggagacggag ggcggctcga aggagatcac
     4921 gcggacgctt tcgaagatcg tcatttggcg cctcctgaga gataggcgtg gtcccgcgcc
     4981 acggcgcggg ccagggcgag catggcggcg ccgtccgacg cggacgagcc gcctccggcg
     5041 gccggtgcgg aggcgccttg cgcgaactcg gcgacggggc cctcgtaggc ggcgcggagg
     5101 gcgaccgcgc cgccctggag aagcgcgaag ccggcgttct tcgaggcctc gagggaggcg
     5161 cggtccgcga agagggtggc cttgccgcgg aagggttcgc cctcgtaggg acagaagagg
     5221 ccgcagttgc cgcactcgtt gcagaggtag tcgacgtgga ggatctggaa ggcctgggcc
     5281 cccgagcccg aggggcgcgg gatggccatg ttggcgcggt tcgggcacac ctccacgcag
     5341 cggaggcagg cggagtcgca ctccaggcag cgctcggcct cgcgggcggc gaagccctcc
     5401 gagccggcgg cgagaggatg ggcgaactcg ccgcgcgagg ccagccgttc ggcccggggc
     5461 tccggcggcg agtagggcgc cagaggcggc tcgacgccgg ccttcctgag gatggcgtag
     5521 gcggcgcggc gtccgtcggc ctccgccgcg atgatggagg cggggccgcg ggcggcgtcg
     5581 ccgccgacgt agagtccggc gacgggcgtc tcctgggtgg cggggtcgta gacgggcctt
     5641 ccgtccttgc ccacgggaat gccgaaggcc ttcatgaggg cggggtcggg cgtctcgccg
     5701 acggccgcga cgaggaggtc gcagtcgagc tcgatcgtct cgtcggaggg gaggggcgag
     5761 cggcggcccg aggcgtcctt ctccccgagg cgcatcttgc gcaaggtgag cttcccgggc
     5821 gcggcccgct cggggaggct gagctcgagg agggcgccct gcccgtgggc ggcgccggcc
     5881 gcgccggagc ctgacgagcc tgcgccggcc gcgccatcga ggacggcggc ctcccgcatc
     5941 gcgttctcga gctcctcgcg gtcggcgggc atctcggcga gggtccgcct gtaggagagg
     6001 cgcacggact cgacggaggg aaggcgggtc gcggcccgca ctgcgtccat cgccgtgttg
     6061 ccgccgccag cgacgacgac gcgcctgtag cccgcgaact cgccggggcc ggccgaggtc
     6121 gcctcgagga aggccagggc gtcgacgacg cggacgccct cgccctcgag cctgagctcg
     6181 cgggccgccg gggctccggc ggccaggaag accgccgaga agccctccgc cttgagggag
     6241 gcgaggtcct tggcgcggaa gccgaagcgg aactcggcgc ccagggcctc gatccttcct
     6301 atgtccttcg cgaggtcctc gcgggagatg cggaagcgcg ggatgacgtt ggccggcacg
     6361 ccgccggccg aggcggcctc gtccaggacg acgaccttcg cgcccgagag ggccaggtgg
     6421 tgggcgcagg cgaggccggc gggccccgct ccgaccacgg cgaccttgcc ggggaaggcc
     6481 gccttcgcgg gcttcccggg cggcaaggag gccgccttcg cgcaggcgag cttgaccgcg
     6541 cggatctcca cggagccctc gtagtcgttc cgcgagcagg cggcctggca gacgtggtcg
     6601 cagagggtgc cggtcacgca gggcaggggg ttgtcggcga ggatcgtctc gagcgcggcc
     6661 ttggcgtcgc ccgaggcggc gcggcggatg tactcgggga ccttctggtt gacggggcag
     6721 gcctcgacgc agggcgcggc gaagcagtcg aagaggggca gggccctctt gatcgacgcg
     6781 ttgcccgact tccactcctt gcggtactcg ggccgctcca gggcggcgtc ggccaggcgc
     6841 gcgatggcct tggcgtcggg ccggccctcg ccgcggttgg cggccgggtt cgcggcgagc
     6901 gcctccgcgg cggcgcgggc catcggcacg agcctcaggt agccgcccgg cttcaggatg
     6961 tccgtcgcga cggtgagggg gcccaggccc gcctccacga ggtcgcgcgc gttcagggcg
     7021 gaggcgccgc cgcagtagga gaggcggcgc gggaagccgg gcagggcctc cgcgaggcgg
     7081 gcggcgaggc gggtcgtgac cgggaagagg gcgcggcccg acatgtagcg ctcggctccg
     7141 ggcaggaagc ccccgtcgtt ctcgttcgcg agggtgttgg agagcttgat gccgaagcgg
     7201 cgcccgttcg cctctgcggc ctcgcccagg ctccggacga gctccagggc gtccttccac
     7261 tggaggtcgt gctcgaaggt ggcgcgcttg aggcctatgt cggtccagcc cgtcgcgtcc
     7321 aggatgcggc gcgcctcgtc gtagcccagg agggtcgggt tgagcttcac gtaggcgtcg
     7381 aagcccttgt cctcgatgag gtagcggccg atgcggccga tctcgtcggg ggggcagccg
     7441 tgcatcgtgg acagggtcac ggagtgcacg ggcttggcgg ggaagtccga gaggagggtc
     7501 ctcgcctttc cgagggcctc ggcgccgaag gcggcggcga aggaggggcc ctcgacgaag
     7561 gcctcgaggt cgcgcatcgc gcggccccag aaggcggtct tctccgggga gcgcatgccc
     7621 tcgatgtatg cgtcgacctt ggccccgcgg atgcccgcca gggtgtagcc gacggacatg
     7681 ttgaagaaga agtcgcgggg cttcgcggac cagagggccg cgaggaggtt gaccgcgatc
     7741 cagccgcgga ggtattcctc gcgggcggcc tcgagggaga gctcggtcga ccactcggtg
     7801 ttgtggccct cgtccagggc gaggatgcag ggcttctcga tctccagggc gtcgttctcc
     7861 tggacggtct tgagctcgaa ggcgcgggag cccgcgaggt aggccgcgac gaggttgggc
     7921 gcgatctggc tgtgcgggcc cgcggcgggg ccgacgggaa ggctcgccct gccgcccatg
     7981 acggcgatgc ccgggctcga ggcctcgagc tcgaagactc cgcggaaggc gcgctcgggg
     8041 atctcgtaga tcgtgccctt cgcgcggtac tcgcccgcga tccgctcgag gagagcggga
     8101 aggggcgtaa cgtccatgac tttgctcacc tgtaccccca cgtgaggaat tttccggagc
     8161 cgggctgggc gacgatgtcg ccagcctcgg cctcgtccgc gcctcgcgcc gcccggtaga
     8221 ccggggcgcc gcgcacgtag gtaacgagga ttcggcccgt caatctcatg ccctcgaagg
     8281 gcgtgatctt gcctttggag aggagcttcc cgccctcgat ccgcgtcgag gcggagggat
     8341 cgacgaggac gaggtcggcg tccttgccga cggcgatgga gcccttgcgg gcggacagtc
     8401 cgtagcggcg cgaggcggcg ccgcccgtcg cgtccaggaa gcgggggagg gagagccgcc
     8461 ccgaaaggag gccctcggag aggaggtagg gcagcatcgt gcccgtgccc gggatgccgc
     8521 cgtaggccgt ccagggattc cccgtgttct tctcctcctc gggggcgggc gcgtggtcgc
     8581 tcgcgacgaa gtcgaccgcg cccgaggcga ggagcctcca gaggccctcg cgctcggccg
     8641 cccccttcac gacgggggcg gtcttgaggg aggccccgag cctcgggaag tcggcgtccg
     8701 tgaaggcgag gtagtgggcg caggtctcgc agctggctcc tcccgcgtgg aggagctccg
     8761 cggcctcggg cacgccgacg tggaccacgt ggagggtctt ctcgcggccc ttggccaggg
     8821 ccagggccga ggcgcaggcg acgagctcgg ccgcggccgg ccggcccgcg gcgtagtcgg
     8881 cccattcggg ggcggccgct ccgcggaggg ccttggccct ggccgtcgcg gccgtcacgt
     8941 agtccaggtc ctcggcgtgg aggaggatgg gccgtccgag ccgctcgccc tcagcgagga
     9001 tcctcgcgaa ggcctcgtgg gtcacgctcg tgaagctgtc catgccggag atgaagtagc
     9061 acttgaagcc gacgaccttg ggggcgagct ccgccatcgc gccgcccgag ccgccgcttc
     9121 ccaggctcgc ctccgccgcg tggccggaga cgccgccgta gaaggcgtag tcgacgaggg
     9181 cggaggggga gacggccgcg agcttgccct ccagggcggc cagggtcgtg accggaggca
     9241 gggaggtgca gggcatgtcg atgacggtcg tcacgccgcc cttcgcggcc tccgccgtgc
     9301 cgtggcggaa gtcctcgcgg tgggtgaagc ccggctcgtc gaagtggacg tgggggtcga
     9361 tggcgccggg gaggacgagg aggcccgagg cgtcgacggt ctcctcgccg gcctccgcct
     9421 ccaggcgtgg ggctatctcg gcgatccgcc cgccgcggac gcggaggtcc aggcggagga
     9481 agtcctcctc cccgggaagg gcgacgaggc cgttttgcac gatcatcgct tccctccctt
     9541 ccccgccttc gcgaggaact ccgggcggct gcccgagagg tagtccaggg ccttctccgg
     9601 cgtgagggga aggtcgtaca tcgcggggcc gtccgggcgg gccgccctga gggcgtccag
     9661 gagggcgaag tagcccgcga tgccgtagac gaggggcggc tcgccgatcg ccttggagtt
     9721 gaggacggcg cgggggttgt cggcctcggg aaggaactcg acgtccacgc cgcttgcgag
     9781 gaagtgcgcg tcggggagct tgtaggtgga gagcgtgtcc gacaggagcc tgccttcctc
     9841 ggagaacttg agctcctcga ggagggacca gccgatgccc tgggcgaagg cgccctcgat
     9901 ctggccgagg tccacggagg ggtcgaggct cctcccgacg tcgtggacga tggccgcgcg
     9961 ctcgatctcg tagtcgccgc gcaggacgtc aaggcggacc acgacgaggg ccgcgccgta
    10021 gacgtggtag gcgaaggggc tgccccgctc ggccttcatg tcgtagaaga ggccgggcgt
    10081 cgcgtagaag ccgtgggcgg agaggtcgat ccgcgcctcg tggcacttct ccacgagctg
    10141 ctcccaggag aggcccgcgt cctcgcagcc gcgcatgagg cggccgtcgc ggatcgagag
    10201 attgctcgcg ggctcgccca cgagcgcctc ggccttcctg aggaggcggg agaggagctc
    10261 ctcgcaggcc acgagggcgg ccatgccgtt gatgtcgctc cccgtgctcg cggccgtggg
    10321 gacggtgttc gcgacggtca gggtcgtcgt ccgctctacc gccacgcgct ccagggggat
    10381 gcccagggtg cgggcgacga cgcgcgcgat cttccgggag acctgctggc ccatctccac
    10441 ggcgcccgtg gagacgagga ccgaaccgtc cgtgtaggcg tggaccaggg ccccgccctg
    10501 gttcatcggg agtttcgtga aggacacgcc gaagcagttg gggaggaccg ccgcgccctt
    10561 cttctccagg ggatgggagg cgttgaaggc gtcgatctcc gcgcgaagcc cgctccaccc
    10621 cgtcttctcg aggaggcggt cgaaggactc cccggccctg acgcccgaca tgggcatccc
    10681 gtagtgggtc gtgtcgccct cggcgatcag gtttttccgc ctgagctcga cggggtcgat
    10741 cccggccttc gccgccaggg cgtccagggc ggcctcgaag acgaagaagc cctggggcgc
    10801 gccgaagccg cggaaggcgg tgaaggaggg gaggttcgtg cggcagaggt agcccgtgac
    10861 gcggacgttg gggacgcggt aggcgcccac cgcgtggaag agggtgcgcg agatgatcgc
    10921 ggcggagagg tcgcaggagc agccggagtt ctggtagtag tcggcctcga aggcggtgat
    10981 ccgcccctcg gcgtccatcg cgatcctgaa gtcgctcgtg tagggatggc gcttcccggt
    11041 cgcgcgcatg tcggtcctgc ggtccaggta gagcttcacc ggcctgccgg agacgcgggc
    11101 gcccagggcc gcgaggcagg cccaggaggt ggcctggtcc tccttgccgc cgaagccgcc
    11161 gcccaggcgc ctcgcctcca cctccacgga gctcatgggc aggcccagga tccgcgccgc
    11221 cgcccgctgt acgacggtgg gtccctgggt gccggagatt atgcggaagc gctccccgtc
    11281 caggggctcg gcgagcgctc cctgcgtctc caggtagacg tgctcctggc cgccggactc
    11341 gacgcggccc tcggccgcga aggcggcgga cgcgaaggcc gcggccgtgt cgccggaggc
    11401 ctgggtcctc ggggggagga tgaactcgcc cttggcggcg gcctcccggg cgtccaggac
    11461 gggctccagg ggctcgccct ccacggcgac gagctcgcag gcccgccggg ctagggactt
    11521 cgtgcgcgcg aggcagagga cgaggggctg gccccagtac tcccactcgt gcgcggggag
    11581 gaggggctcg tcggccacgg tgaagccgag ctggttctcg ccgggcacgt ccgcgtgggt
    11641 caccacccgc acggaggggt ccagggccag ggcggccgag gggtcgatgc gcgttatgcg
    11701 gccgcgggcg ctctccgaga gcttgaccgc ggcgtggagc aggctcctcg gctcgggcag
    11761 gtcgtcgatg taggcggtct tgcccctcac gttgagggaa gcctccggga tggccttcac
    11821 ttgaggacct ccttagccac gccctccgcg agggcgcggt cctcggggaa cagggagacg
    11881 aaatgcgcga gcacgaggcg ctccaggacc ctgatccggt agcccgccga gccgcggacg
    11941 tcgccgatgg gcgagacctc ggagacggcg gagcgggccg cctccacggc ggtggccgcg
    12001 gagacctcgc ggccggccag gaggtcggag gtggcctcca ggaggagggg cgtcgcggcg
    12061 acgccgcccg ctgagatccg ggcccgcgtt atgcgcctcc cgtccagctc cagggccgcc
    12121 gcggtgttca ccgccgcgat gtccaggctc ttccgcttgg aggccttctc gaagctgagg
    12181 cgcaaggccc ccgcgacggg tatgaggaag gccgcgacga tctcgccggg ggcgaggtcg
    12241 aggcgcttgt agccgaggta gagccgctcg agcgcgagct ccctcggggc ctggcccgcc
    12301 cgctccaggc ggagggaggc gcccagggcg acgaggatgg aggtcatgtc ggcgacgggg
    12361 gaggcgttga cgacgttgcc gcccagggtc gcgcggttgc ggattaggac gctcgcgaac
    12421 cggccctcga aggcctcgac gccgggcagg gcggcccgga cctcgggcga ggcgaagaag
    12481 tcgcgcaccg tgacggccgc gccgacctcg aggacggagc cgcgccgctc gatcgccttg
    12541 aggcggggat cggcgtcgag gaggcggaag ccctcgcggg gctcgggatc ggtgttgcgg
    12601 acgtagaagt cggtgccgcc gcccaggatg agcgcgggaa ggggcgagcc ggcggcgggc
    12661 aaggctccgc ctgaaggcgc ggcgccggcc gccggggccg aagcggccgc gtgggccggc
    12721 gcggcgccat cggagcccgc gggcgcggcg gggaggagcc tgccgtccgc gaaggcctcc
    12781 agggaggcgg gcacgacgcc ggccttggcc agggcggcga gcctcgcggc gggctctgag
    12841 gggaggtccg cgaattcctt gaggatgcgg gaggccgcgc ggcggatcga gccgtagccc
    12901 gtgcagcggc agaggttgcc ctcgaccgcc tccagggccc cttcctcgtc catgcgccgt
    12961 cccgcgagga gccaggccgt gagggagatg atgaagcccg gggagcagaa gccgcactgg
    13021 ctcgcgttct cctccaggaa ggcccgcatc acgggcgtga ggcccttctc caggcccgcg
    13081 gcggctgcgc cggccgacag gccctctatc gtgacgaggt gcctgccctc gagctcgccc
    13141 agggcgagga tgcaggaggg cacggccttg tagacggccg ccgcgccgga gccgcccgcc
    13201 aggggcttct cgccgacgag gacggcgcag gcgccgcagt cgccctcgcg gcagccttcc
    13261 ttcgcgcccg tgaggcccag gtcaccgcgt atgaagtcga gggcgggcct tcccgcccag
    13321 tcgcccgtct ccacgacgag gtcgttcgcc aggaatcgca aggttctact catggctttc
    13381 cttcgttgcc gcgggcccta agtctaggac ttggcctggg cgctttcaat cgaggccggg
    13441 cattcccgcc ccgccccgct cgtggcctgg aaatcgacga gggtctcctc ggtgcagcgc
    13501 tcccagtgcg ccccgaggag gacgacggcg ccccgcctgt ccccccgctc cagggcggcc
    13561 aggatggcct cgtgctcctc catggactcg gagacgagct ccgagacctg catgaaggcg
    13621 tattcgtatc ggaagaggat gcgcttggag tcggccagga cccgcatgag gtagcggttg
    13681 ccgttgtcct cgaccagggc cttgtgccag gcggagtcga gttcgatgcg gcgcaggggg
    13741 tcggccccct cctgcttcat ggagcggtcc agctctcgga ggagggtgag cttctcgggg
    13801 gaggggaggg gggaggcctt gagggcggag cactcgagga tccgtacgag cggatacact
    13861 tccttgatct ccgactcctc caggcgcttg acggaaaagc ccatgtgctg ctgcgaggag
    13921 atgaagccct cgcgctccag gcggaggagg gcctccctga cgggagtccg gctcacgccc
    13981 aggcgctccg cgagctccga gtccttgagc ctctggccgg gggcgaactg tccccggatt
    14041 atctgcccca ggagttcctg gtggacgtcg gcgctgaggc gttctcggga gatcgacata
    14101 ggaatactgt attctgtata caaatatccg tcaagttttt ctttgaagaa atcggctcgc
    14161 ggccgcttcc ccggggcgcc cggggaaccg cctgaaggcg ctgaaagcgc cgtcggtagc
    14221 cgggcc
//
LOCUS       DAOOSJ010000033        14084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2899_length_14084_cov_5.450994, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000033 DAOOSJ010000000
VERSION     DAOOSJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14084
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_14084_cov_5.450994"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLG14_02950"
     CDS             <1..408
                     /locus_tag="PLG14_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOX48118.1"
                     /translation="LGVSEEVVRRHPFPGPGLGVRILGEVTKARCDTLREADAIYIAE
                     LKRRGLYDRIWQAFSVLLPLRSVGVAGDERKYGSVLALRAVVSEDGMTADVYPFEGKD
                     LLAISAAITNRVPEIGRVVYDVSSKPPATIEWE"
     gene            complement(581..1153)
                     /locus_tag="PLG14_02955"
     CDS             complement(581..1153)
                     /locus_tag="PLG14_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48119.1"
                     /translation="MRPEARFAVPAAALLMALLAGGCAGTAPGRAKPPAAPTAPGAPR
                     PSASGPARPAPRTLPGFAELPPEALAFAEDFRKKVELGDWKAIGASADQAFKAAHPGT
                     SPAEGAYLARLLSVGAQAGGAAPFDEEGPRPAAFRPAEARRAVFESAELRGPVALVYG
                     RLERKSGEPLPFVLRVLWRLEPPRILGEAP"
     gene            complement(1150..2235)
                     /locus_tag="PLG14_02960"
     CDS             complement(1150..2235)
                     /locus_tag="PLG14_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX48120.1"
                     /translation="MPAIRVLLVEDDEAHAELMARAFAKDQGAWELERASTLAGARRS
                     LAGPGFGLVIADFRLPDGEGLELLASADGEPALPVLVLTSHGSEAVAADSIKRGALDY
                     VVKSPESFAAMPGQARKALRAWALLEEKREAESRLRRSLAEKEVLLKEVHHRVKNNFQ
                     IVSSLLSLQAEAVGDGAARDALLESEQRIRSMALIHEKLYESDDYARIDFPDYLRSVA
                     ARLEAAYRAPERGIGIGFETEPVDLSLETAIPLGLVLNELVTNALVHAFPEGKKGSIS
                     ISFRKCGEGAVLAVADDGVGLPAGFDLERQKSLGCQLVRMLSLQAGGRPEVETGPGGT
                     RISIRLAPAPGAGSSANSSAGGEDRTA"
     gene            complement(2242..2694)
                     /locus_tag="PLG14_02965"
     CDS             complement(2242..2694)
                     /locus_tag="PLG14_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48121.1"
                     /translation="MDEKGRKRLVLLVEDNDDHAELVARCFERRRARLSLERLPDGEA
                     ALAWLEARKAKAPTGPDGLPDLILLDLRLPKVDGMEVLDRIKADEALRAVPVVVLSSS
                     DAEADVLRAYGRHANSYVVKPLDFEALKEALEGLGIFWLDINRGRREG"
     gene            complement(2699..4261)
                     /locus_tag="PLG14_02970"
     CDS             complement(2699..4261)
                     /locus_tag="PLG14_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOX48122.1"
                     /translation="MDDTQNPGGEEAADSKSPPPAEAPQGPTEKAAPEKAQPEQAAPE
                     KGQPEKGAAEAIDLGSSVSFRSASRPNSGYAEIAVAGDGMTATAHFYPPQGEEGPPLQ
                     AEDLGELFARLGVVSGILWDEIGEALMRCNLERRPLRDLVVARGSLPEDEIPAHAALE
                     PRFRRNGPLVPEDAQRVDYRQLSSLTVVKKGELIARQVPRGEGKAGLDVRGFEKPFAR
                     RKIENFLPGKNCSLTEAGIESSVDGLLAVNGDRLDVEEILLVKGDVDYHTGHIVFPGD
                     VVIEGRIGDGFKVFSGGSVISKATMDAYDVSAKKDLLCAQGLIGRRGGQVRVGGELRA
                     RFIQNCKVAVRGDIHVANAVVGSRIYTLGTIDLGDKGVLMGGESYALHGLRCGRLGNE
                     ANQRTIVHVGTDFTVQQQLDQANERLRALAFKAKRAAEAAATRPSPGVLRLKEAVDAS
                     MVELSTLIGRLLLSLDADDAAVVEVRGDIFPGTTVEICRLSIVVDQPLKACRFRLDKT
                     AGRVVAERLGGS"
     gene            4456..4932
                     /locus_tag="PLG14_02975"
     CDS             4456..4932
                     /locus_tag="PLG14_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOX48123.1"
                     /translation="MKERLERLKAVRVIIKSNRIDSQDQLLQLLAEQGFSVTQATLSR
                     DLKYLKVGKVSDGHAGYYYTVPSDEERRESERHYIQDFLRGYVSIDWNLNMAVVRTFS
                     GHSDSVALAIDNMGLEEVLGTIAGRDNVVFVALREGVTGEAFIEAMKKKVPDLEAE"
     gene            complement(4994..5203)
                     /locus_tag="PLG14_02980"
     CDS             complement(4994..5203)
                     /locus_tag="PLG14_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HOX48124.1"
                     /translation="MEEKIVILHGFTGDEAVALMRAVKAALPAAKEAAFATSTPTNLD
                     WKLKDLVEHVAEEHRQFREQNARKA"
     gene            5461..8049
                     /gene="clpB"
                     /locus_tag="PLG14_02985"
     CDS             5461..8049
                     /gene="clpB"
                     /locus_tag="PLG14_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOX48125.1"
                     /translation="MDYDKFTLKAQEAVQDASGEARKRDHSQVELEHLLLALLRQEEG
                     VVPALVGRVGADRAELEAELEASLAKKPKVFGEAAQIYLSPAASKALAKAEAEAAALK
                     DEFVTAEHILLAIAGAEGPTGDSLRSRGLTKAAILEALKSVRGNRRADDQEAEEKYRV
                     LERYCRDLTALARAEKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAVVEG
                     LARRVVAGDVPDSLKGKKLLALDLGALVAGSKFRGEFEERLKAVIEEIQKSDGKVILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELRCIGATTLDEYRKHIEKDAALERRFQ
                     PVLCVEPSVEDSIAILRGLKERYEVHHGVRIRDEALIAAATLSDRYITSRFLPDKAID
                     LVDEAASRLKMEIESQPTELDKAERRILQLTIERQALQKETDPASLDRLSRLEKEIGE
                     TQASRDAMRLRWEGEKAKIQEIRALKLSLEELKLEGERWEREGNLAKAAEVKYGKILE
                     AQKKLDEVTAAMKEEPADARLLREEVSEEDIARVVSTWTGIPVSKMLGSEAQKYLELE
                     ALLERRVVGQDAAVKAVADAIRRNRAGLSDPNRPLGSFLFVGPTGVGKTELAKALAEF
                     LFNDEKSLTRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPDVFNVLLQLLDDGRLTDGQGRQVDFKNTIVIMTSNLGSDLILEAKDMKEVKAG
                     VQALLKTSFKPEFLNRIDETVVFERLGKDRILAIVDIQLARLAERLAARNIRVSLSPA
                     AKAHIAEAGYDPLYGARPLKRAIQALLQDPLAAGILSGRVKEGQEIVVDAEGEGLGFK
                     TRAKKA"
     gene            8049..8930
                     /locus_tag="PLG14_02990"
     CDS             8049..8930
                     /locus_tag="PLG14_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX48126.1"
                     /translation="MYTDSHAHLSSVAEELGEDALAGVLSAYARAWEAALPGIPAAAS
                     APGPAGPAGARGRGRPAPLLVDIGTEPGDYAARRALLVRLSGGAALPPWLRMSLGVWP
                     GREALAEPRAALVRLTDALAAAGAEAAAIGECGLDYHHMDGSRAAQAELFEGQIGLAR
                     ERGLALVVHSREAFEDTRDILRAARLASPVLIHCFGYGRKEAEAFLELGAYLSFAGNL
                     TYKKAEALREALAAAPAERLLLETDAPYMNPEPRRGRPSSPSDIARSYEAAAALRGEA
                     AESLAASVAANARLLFG"
     gene            complement(8927..9490)
                     /locus_tag="PLG14_02995"
     CDS             complement(8927..9490)
                     /locus_tag="PLG14_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HOX48127.1"
                     /translation="MTLKAQGRAGKAGPFLAILAILSLAALPLPAQELAPQAAVPKAD
                     GLVSPGEYGYETAYAGMRLSAALSQDGKILHLALSAPTKGWAAIGLGSRKMDGAHMIL
                     GYDAAGSAAVSEEKGKGRSHSPTQDKRLLSSAVKESGDATVLEFSLPLADLAPSGKLP
                     LIIAYGARDDFRSMHSKYGSFDLPLKR"
     gene            complement(9641..10825)
                     /locus_tag="PLG14_03000"
     CDS             complement(9641..10825)
                     /locus_tag="PLG14_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48128.1"
                     /translation="MAQELDYFEFLRRVYFFKDLADDEIRLVEGSCAEEEYAAGDVLF
                     VEGSTADRFYIVIEGRVEVWKNFYDPKPDLLAVHGPGHFFGEMALVDELPRSATVVAK
                     DPVRVLYLYRDDFRRLIKERSSIALSVMTSISFMVRSSNEVFVDDLRRRNAELERAYA
                     DLEKAQAERLRTERLSTLGKFSSLILHDIRNPLSILKGQLQLMEIQADEAPEKARARI
                     KASLAEVARLERLASEFLDYSRGEIRLDMAIARPSSLLFRIEEGLRDRLAERGIACEL
                     DAAYDEPVILDEERVARALHNVADNARKAMAAKGGVLRMGSRREGDLLVFEVADSGEG
                     MSPEVLSRVFEPFYSSSGQGGTGLGMLIVKNIVEAHGGSVSLQSAPGEGTRVSLSFPL
                     RA"
     gene            complement(10890..11657)
                     /locus_tag="PLG14_03005"
     CDS             complement(10890..11657)
                     /locus_tag="PLG14_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOX48129.1"
                     /translation="MKISTKALLAVAVLASAFAASAFAQGAAAPAEIGAPDPAKIGAD
                     TAQQKLKEVSVDKFEAAGFWNAYIAADDGYIQARLFEGGPAGKAAEPIPDERYIGIDP
                     KIADKYALGVRTDFYRRGYNELMIVAKRPIPVEGITKTISVWVVGRNYNHTLKIVLQD
                     FFGQRFELPLGKLNFQGWKKLSVAVPPQNPDGQSGIVQRNFHYTSHMGLKIVGFKVEC
                     DPMEAFGTYYLYFDDLRAVTDLFAEDDRDPDDMSDTW"
     gene            complement(11670..12458)
                     /locus_tag="PLG14_03010"
     CDS             complement(11670..12458)
                     /locus_tag="PLG14_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOX48130.1"
                     /translation="MKQSVIRAFCLFLVLLSGIGVGSAFADENTVNLESVVIQSFDDP
                     AKEPWFLIGSKFSTAGFPRESYVKTWPLALYGSNPEGKDLKALGIATLYDRKEYNWVD
                     LVPGKKSGEGENVKYEPVELPLPGRVKAIDMWVWSGNYNYYIEAYIRDYKGIVHILPM
                     GELNFIGWKNMRVNMPDNVPQSKKYLPKRESLSLVKFRIWTRPTENVAILTNDPNSPD
                     IDKAVKFYFDNLKVLTDTFEGLFDGDSLQRPDVIKEAWGSGEKK"
     gene            complement(12706..13821)
                     /locus_tag="PLG14_03015"
     CDS             complement(12706..13821)
                     /locus_tag="PLG14_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48131.1"
                     /translation="MHSRPTRRPPLPGRAPGLAPKAAVLLLSCALLSCLLPACGPRRR
                     VLVGLDEAFAAARPELARRLFRLSASRPAAVGLAEGPSKLYEAARAAGVAGSGASGPR
                     VLVASPLLASALLGSAAGFGPDGAGAEGPLDGALIVAPEWQGPLPAGLVAASTDYEPA
                     YRAAGRAAGAYVASLRAAGAAEATGAILFSESAARPRVLGEAFAEAFEAEAGFPPLSP
                     PLPPAPPAGAAAPQAGPEAEADAALQALLGSDLRILFVAAGPGSAGLCARAARPGLAL
                     GAAAPLSGCPEACFSVEPDEAALARAVEDLARGAGLAASRSPQGGAARPAQDGAGDTV
                     SRLVLVPALLGRARSAEGFRAGESSLAALLRRAAGGR"
     gene            13945..>14084
                     /locus_tag="PLG14_03020"
     CDS             13945..>14084
                     /locus_tag="PLG14_03020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOX48132.1"
                     /translation="MTSYKELGLVNTVELFKKAVKGGYAIPAYNFNNMEQLQAIVQAC
                     VET"
BASE COUNT         2078 a         4844 c         5106 g         2056 t
ORIGIN      
        1 ctgggcgtct cggaagaggt cgtgcgccgg caccccttcc ccggacccgg cctgggcgtg
       61 cgaatcctgg gcgaggtgac gaaggcccgc tgcgacacgt tgcgcgaagc cgacgcgatc
      121 tatatcgccg agctgaagcg gcgcggcctc tacgaccgca tctggcaggc cttctcggtg
      181 ctcctgccgc tgcgctcggt gggcgtcgcc ggcgacgagc gcaagtacgg atccgtgctc
      241 gcgttgcgcg cggtcgtatc cgaggacggc atgaccgcgg acgtctatcc cttcgagggg
      301 aaggacctcc tcgcgatctc cgccgcgatc acgaaccgcg tccccgagat aggccgcgtc
      361 gtctacgacg tctcgtcgaa gccgcccgcg accatcgagt gggagtgaaa gcccgcgccg
      421 ccggcgcggg cgatcgacgc gaagggccgc ccctcggggc ggcccttcgt ctatttgcgg
      481 cggtccgcgc cgtgccggcg cgcgtggttc ctgcccgcca ggcgccgcgc cggcgcgctt
      541 gctctcggcg ccgcgcgcga cgcgggcgcc gaccgcgcct ttagggcgct tcgccgagta
      601 tccggggcgg ctcgagccgc cagaggacgc gcaggacgaa ggggaggggc tcgcccgact
      661 tgcgctccag ccggccgtag acgagggcga cggggccgcg gagctcggcg ctctcgaaga
      721 ccgcgcggcg cgcctcggcc gggcggaagg ccgcgggacg cgggccctcc tcgtcgaagg
      781 gcgccgcgcc ccccgcctgg gcgccgaccg agaggagtcg ggccaggtag gctccctccg
      841 ccggggaggt gccgggatgg gcggccttga aggcctggtc ggcgctcgcg cctatggcct
      901 tccagtcgcc aagctccacc ttcttccgaa agtcctcggc gaaggccagg gcctcgggcg
      961 gaagctcggc gaagcccgga agggtccgcg gcgcgggcct ggcggggccg gacgcggatg
     1021 gtcgcggcgc gcccggggcc gtaggcgctg cgggcggctt ggcccggccg ggggccgtgc
     1081 ccgcgcagcc cccagcgagg agggccatga gcagggcggc ggccgggacg gcgaacctcg
     1141 cttcgggcct catgccgtac gatcctcccc gccagcgcta gagttagcac tgctgccggc
     1201 gccgggggcg ggggcgaggc ggatggagat acgcgttccg ccgggacccg tctccacctc
     1261 gggacggccc cccgcctgga gggagagcat cctcacgagc tggcagccga ggctcttctg
     1321 cctctccagg tcgaagcccg caggcaggcc cacgccgtcg tcggcgaccg cgaggacagc
     1381 cccctccccg cacttcctga aggagatgga gatcgagccc ttcttcccct cggggaaggc
     1441 gtggaccagg gcgttggtga cgagctcgtt gagcacgagg ccgaggggga tggccgtctc
     1501 gagcgagagg tcgacgggct cggtctcgaa gcctatgccg atgccccgct cgggggcgcg
     1561 gtaggcggcc tcgagccgag ccgcgacgga gcgcaggtag tcggggaagt cgatccgcgc
     1621 gtagtcgtcg ctctcgtaga gcttttcgtg gatgagggcc atgctcctga tgcgctgctc
     1681 gctctccagg agagcgtcgc gggccgcgcc gtcgcccacc gcctccgcct ggagggagag
     1741 gaggctggag acgatctgga agttgttctt gacgcggtgg tggacctcct tgaggaggac
     1801 ctccttctcc gcgaggctcc gcctgagcct cgactcggcc tcgcgcttct cctcaaggag
     1861 ggcccaggcc ctgagcgcct tccgcgcctg gccgggcatg gcggcgaagc tctcggggct
     1921 cttcacgacg tagtcgaggg cgccgcgctt gatggagtcc gccgccacgg cttccgagcc
     1981 gtggctcgtg aggacgagga cggggagggc gggctcgccg tccgccgagg cgaggagctc
     2041 gaggccctcg ccgtcgggga ggcggaagtc ggcgatgacg aggccgaagc ccggccccgc
     2101 gagggagcgc cgggctcccg cgagggtgga ggcccgttcg agctcccagg ccccctggtc
     2161 cttcgcgaag gcccgggcca tgagctcggc gtgggcctcg tcgtcctcga cgaggaggac
     2221 tcggatcgcg ggcaccgccg cctagccttc ccgcctgccg cggttgatgt cgagccagaa
     2281 gatcccgagg ccctccaggg cctccttcag ggcctcgaag tcgaggggct tcacgacgta
     2341 ggagttggcg tgcctgccgt aggcgcgcag gacgtcggcc tcggcgtcgg aggagctcag
     2401 gaccacgacc ggcacagccc tcagggcctc gtccgccttg atcctgtcca ggacctccat
     2461 gccgtcgacc ttcgggagcc gcaggtccag gaggatgagg tcgggaaggc cgtccggccc
     2521 cgtcggggcc ttcgccttgc gggcctcgag ccaggcgagg gccgcctcgc cgtcgggcag
     2581 gcgctcgagg ctcaagcggg cgcgcctccg ctcgaaacag cgggcgacaa gctccgcgtg
     2641 gtcgtcgttg tcctccacga ggaggacgag ccgtttcctg cctttttcgt ccatgtccct
     2701 aggatcctcc gagccgctcg gcgacgaccc tgccggccgt cttgtcgagc ctgaagcggc
     2761 aggccttgag cggctggtcc acgacgatgg acaggcggca gatctcgacg gtcgtgccgg
     2821 ggaagatgtc tccgcggacc tcgacgacgg ccgcgtcgtc ggcgtccagg gagaggagga
     2881 ggcggccgat gagggtggag agctccacca tggaggcgtc caccgcctcc ttgagccgca
     2941 agactcctgg gctcggccgg gtcgccgccg cctcggccgc tcgcttggcc ttgaaggcca
     3001 gggccctgag cctctcgttc gcctggtcga gctgttgctg gaccgtgaag tccgtcccga
     3061 cgtggacgat ggtccgctgg ttggcctcgt tccccaggcg gccgcagcgg aggccgtgga
     3121 gggcgtagct ctcgccgccc atgaggacgc ccttgtcgcc caggtcgatg gtgccgaggg
     3181 tgtagatgcg gctgcccacg acggcgttgg ccacgtggat gtcgccgcgg accgctacct
     3241 tgcagttctg gatgaagcgg gccctgagct cgcccccgac cctgacctgg ccgccgcggc
     3301 ggccgatgag gccctgggcg cagaggaggt ccttcttcgc gctgacgtcg taggcgtcca
     3361 tcgtcgcctt ggagatcacc gagccgccgg agaagacctt gaagccgtcg ccgatcctgc
     3421 cctcgatgac gacgtcgccg gggaagacga tgtggcccgt gtggtagtcg acgtcgccct
     3481 tcacgaggag gatctcctcc acgtccaggc ggtcgccgtt gacggcgagg aggccgtcca
     3541 cggaggactc gatccccgcc tcggtgaggg agcagttctt tccgggcagg aagttctcta
     3601 tcttgcgccg ggcgaagggc ttttcgaagc ctcggacgtc gagtccggcc ttgccctcgc
     3661 cgcggggcac ctggcgggcg atgagctcgc ccttcttgac gacggtgagg gaggagagct
     3721 ggcggtagtc gacccgctgc gcatcctcgg gcacgagggg gccgttccgc ctgaagcggg
     3781 gctccagggc ggcgtgggcg gggatctcgt cctcgggaag cgagccccgc gcgacgacca
     3841 ggtcgcgcag gggcctgcgc tccaggttgc agcgcatcag tgcctcgccg atctcgtccc
     3901 agaggatgcc ggagacgacg ccgagcctgg cgaagagctc gcccaggtcc tcggcctgga
     3961 ggggggggcc ttcctcgccc tgaggagggt agaagtgcgc ggtcgccgtc atgccgtccc
     4021 cggcgacggc gatctccgcg tagcccgaat tgggacgcga ggcgctcctg aaggagaccg
     4081 aggaacctag gtctatcgcc tcggccgcgc ccttctcagg ctggcccttc tcgggcgcgg
     4141 cttgttcagg ctgggccttc tcgggcgcgg ctttctccgt cgggccctgc ggggcttcgg
     4201 cggggggcgg cgacttgctg tccgcggcct cttcgccgcc cgggttctgg gtatcgtcca
     4261 tcctcatcct cgagggccgc cttgggcgcc gccggctcca ggccgccggc gcgaggcccc
     4321 gcatccccaa gtatcggcga aaagccgccg catatcgagc ccgaagccgc ggcgggccgc
     4381 tccggcttgc ccaacgcgcg ccgatatgcg ataataacgc ggaattccgc attttcattc
     4441 atttccggag ccagcatgaa ggaacgccta gaacgcctca aggccgtacg ggtcatcatc
     4501 aagtcgaacc gcatcgactc ccaggaccag ctcctccagc tcctggccga gcagggcttc
     4561 tccgtgaccc aggcgaccct ctcccgcgac ctcaagtacc tcaaggtcgg caaggtctcc
     4621 gacggccacg caggctacta ctacaccgtc ccctccgacg aggagcgccg cgagtccgag
     4681 cgccactaca tccaggattt cctgcgcggc tatgtctcca tcgactggaa cctgaacatg
     4741 gccgtcgtcc gcaccttctc ggggcattcc gactcggtgg ccctggccat cgacaacatg
     4801 ggcctggagg aggtcctggg caccatcgcg ggccgggaca acgtggtctt cgtcgccctc
     4861 cgcgagggcg tcaccggcga ggccttcatc gaggcgatga agaagaaggt ccccgacctg
     4921 gaggccgaat aggctccggg gaggcgctct ccgcgcctcg cgccgctcca gcttcgcttt
     4981 cccgccccct cggctaggcc ttcctcgcgt tctgctccct gaactgccgg tgctcctcgg
     5041 ccacgtgctc gaccaggtcc ttgagcttcc agtccaggtt ggtcggcgtg ctcgtcgcga
     5101 aggcagcctc cttcgcggcc ggcagggccg ccttgaccgc ccgcatgagg gccaccgcct
     5161 cgtcccccgt gaatccgtgc agaatgacga tcttctcttc catgccccga gtgtagggcc
     5221 cgggacgggc tcggcgcaag cggatcctct cgcggggccg ccgcgcggag cggcagcgcg
     5281 gggccgtagc ggggggccgt agcggggagc cgtagcgggg ggccgtagcg gggccgcggc
     5341 gcggagctgc cgcgcggcgg ccccctcccg cgccaaggcg cgccctcggg ctagccgcgc
     5401 cggcggcctt gccccgccct tgggcgaggt agtacctttc ctggaaacgg aaggataacc
     5461 atggactacg acaaattcac cctgaaggcc caggaggccg tccaggacgc ctccggcgag
     5521 gcccgcaagc gcgaccattc ccaggtggag ctcgagcacc tcctcctcgc cctcctcagg
     5581 caggaggagg gcgtcgtgcc cgccctcgtg ggccgcgtcg gcgccgaccg cgcggagctc
     5641 gaggccgagc tcgaggcctc cctcgcgaag aagcccaagg tcttcggcga ggcggcgcag
     5701 atctacctct cgcccgcggc ctccaaggcc ctcgccaagg ccgaggccga ggccgccgcc
     5761 ctcaaggacg agttcgtcac cgcggagcac atcctcctcg cgatagccgg cgccgagggg
     5821 ccgacgggcg actccctgcg gagccgcggc ctcaccaagg ccgccatcct ggaggccctc
     5881 aagtccgtgc gcggcaaccg ccgggccgac gaccaggagg cggaggagaa gtaccgcgtc
     5941 ctcgagcgct actgccgcga cctcaccgcc ctcgcgcgcg cggagaagct cgacccggtg
     6001 atcggccgcg acgaggagat ccgccgcgtc atgcaggtgc tctcgcgccg cacgaagaac
     6061 aaccccgtcc tcatcggcga gccgggcgtc ggcaagacgg ccgtcgtcga gggcctggcc
     6121 cgccgcgtcg tcgccggcga cgtccccgac tccctcaagg ggaagaagct cctcgccctg
     6181 gacctcgggg ccctggtcgc gggctcgaaa ttccgcggcg agttcgagga gcggctcaag
     6241 gccgtcatcg aggagatcca gaagtccgac gggaaggtca tcctcttcat cgacgagctc
     6301 cacaccctcg tgggcgcggg cgcggctgag ggcgcgatgg acgcctccaa cctcctcaag
     6361 cccgccctgg cgcggggcga gctccgctgc atcggcgcga cgaccctgga cgagtaccgc
     6421 aagcacatcg agaaggacgc cgccctggag cgccgcttcc agcccgtcct ctgcgtggag
     6481 ccctccgtgg aggactccat cgccatcctg cgcggcctca aggagcgcta cgaggtgcac
     6541 cacggcgtcc gcatccgcga cgaggccctg atcgccgccg cgaccctctc cgaccgctac
     6601 atcacgagcc gcttcctccc cgacaaggcc atcgacctgg tcgacgaggc cgcgagccgc
     6661 ctcaagatgg agatcgagag ccagccgacg gagctcgaca aggcggagcg ccgcatcctc
     6721 cagctcacga tcgagcgaca ggccctccag aaggagaccg atccggcctc cctggaccgg
     6781 ctctcccggc tggagaagga gatcggcgag acccaggcct ctcgcgacgc gatgcgcctc
     6841 cgctgggagg gcgagaaggc gaagatccag gagatccgcg ccctcaagct cagcctggag
     6901 gagctcaagc tcgagggcga gcgctgggag cgcgagggga acctcgccaa ggccgcggag
     6961 gtgaagtacg gcaagatcct cgaggcgcag aagaagctcg acgaggtgac ggccgcgatg
     7021 aaggaagagc cggcggacgc ccgcctcctc cgcgaggagg tctccgagga ggacatcgcc
     7081 cgcgtcgtct ccacctggac gggcatcccc gtctccaaga tgctcggcag cgaggcccag
     7141 aagtacctcg agctcgaagc cctgttggag aggcgcgtcg tcggccagga cgcggccgtg
     7201 aaggccgtgg ccgacgcaat ccgccggaac cgcgccggcc tctccgaccc gaaccgcccc
     7261 ctcgggtcct tcctcttcgt cggcccgacg ggcgtcggca agaccgagct cgccaaggcc
     7321 ctggcggagt tcctcttcaa cgacgagaag tccctgacgc gcatcgacat gtcggagtac
     7381 atggagaagc actcggtgag ccgcctcatc ggcgcgcccc ccggctacgt cggctacgac
     7441 gagggcggcc agctcacgga ggcggtgcgg cgcaggccct actcggtcgt cctcttcgac
     7501 gagatcgaga aggcccaccc cgacgtcttc aacgtcctcc tccagctcct ggacgacggc
     7561 cgcctcacgg acggccaggg caggcaggtc gacttcaaga acacgatcgt catcatgacc
     7621 tcgaacctgg gatcggacct catcctggag gcgaaggaca tgaaggaagt gaaggcgggc
     7681 gtccaggccc tcctcaagac gagcttcaag cccgaattcc tcaaccgcat cgacgagacg
     7741 gtcgtcttcg agcggctcgg caaggaccgg atcctcgcga tagtcgacat ccagctcgcc
     7801 aggctggcgg agcgcctcgc ggcgcggaac atccgcgtct cgctcagccc cgcggcgaag
     7861 gcgcacatcg cggaggcggg ctacgacccg ctctacggcg cgcggcccct gaagcgcgcc
     7921 atccaggccc tcctccagga tcccctggcc gcgggcatcc tctccggccg cgtcaaggag
     7981 ggccaggaga tagtcgtcga cgccgaaggg gaggggctcg gcttcaagac cagggccaag
     8041 aaggcctgat gtacaccgac agccacgccc acctttcctc cgtcgcggag gagctcggcg
     8101 aggacgccct cgcgggcgtc ctttccgcct acgcgcgggc ctgggaggcc gcgctcccgg
     8161 gaatccccgc ggccgcctcg gcgcccggcc ccgcgggccc ggccggcgcc cgggggcggg
     8221 gccggccggc cccccttctc gtcgacatcg gcaccgagcc cggggactac gccgcgaggc
     8281 gtgccctcct cgtccggctc tcaggcggcg ccgccctccc gccctggctc aggatgagcc
     8341 tcggggtctg gcccggcagg gaggccctgg ccgagccccg cgcggccctt gtccgcctca
     8401 ccgacgccct cgcggccgcg ggcgcggagg cggcggccat aggcgagtgc ggcctcgact
     8461 accaccacat ggacggaagc cgcgcggccc aggccgagct tttcgagggg cagatcggcc
     8521 tcgcgaggga gaggggcctc gccctcgtcg tccactcccg cgaggccttc gaggacacga
     8581 gggacatcct cagggccgcg cggctcgcct cgccggtcct tatccactgc ttcggctacg
     8641 gaaggaagga ggccgaggcc ttcctcgagc tcggcgccta cctctccttc gcgggcaacc
     8701 tcacctacaa gaaggcggag gccctgcgcg aggccctcgc ggccgcgccc gcggagcgcc
     8761 tcctcctcga gaccgacgct ccctacatga atcccgagcc ccggcggggc aggccttcct
     8821 cgccctccga catcgcgcgg agctacgaag cggccgctgc cctccgcggc gaggctgcgg
     8881 aaagcctcgc cgcctcggtc gcggccaacg cgcggctcct cttcggctag cgcttcaggg
     8941 gcagatcgaa gcttccgtac ttggagtgca tgctccggaa atcgtcgcgc gcgccgtagg
     9001 cgatgatgag gggcagcttg cccgaaggcg ccaggtcggc gagggggagg gagaactcga
     9061 ggaccgtcgc gtcgccgctc tccttgacgg cgctcgagag gagccgcttg tcctgggtgg
     9121 gcgaatggct gcgtcccttg cccttctcct cgctgaccgc ggccgagccc gcggcgtcgt
     9181 agcccaggat catgtgcgcc ccgtccatct tgcgcgagcc caggcctatc gccgcccagc
     9241 ccttggtcgg ggcggagagg gcgagatgga ggatcttgcc gtcctgcgag agggcggccg
     9301 agagcctcat gccggcgtag gccgtctcgt agccgtattc gcccggcgag acgaggccgt
     9361 cggccttggg cacggcggcc tgaggcgcga gttcctgggc gggcagggga agggcggcga
     9421 gtgagaggat ggcgaggatg gcgaggaagg ggcccgcctt gccggcgcgg ccttgggctt
     9481 tcagggtcat gttcgctcct tgtccggcag gccctgggag ggcggagccg gatcgccccg
     9541 cttcccgccc tgccgtcatg gatcgaaggt actgacggac gcggaaaaag gggaggaaaa
     9601 accgaccgaa gccgtcaggc atggggcggc ctgccggctt ctaggcccgc aaggggaagg
     9661 agaggctgac gcgcgtgccc tcgccggggg cggactggag gctgaccgag ccgccgtggg
     9721 cctcgacgat gttcttgacg atgagcatcc ccaggcccgt cccgccctgc ccggaggagg
     9781 agtagaaggg ctcgaagacg cgggagagga cctcggggct catgccctcg cccgaatcgg
     9841 cgacttcgaa gacgaggagg tcgccctccc gcctcgaacc catccgcagg acgccgccct
     9901 tggccgccat ggccttgcgc gcgttgtccg ccacattgtg gagtgcgcgg gccacccgct
     9961 cctcgtccag gatgacgggc tcgtcgtagg cggcgtcgag ctcgcaggcg atgccgcgct
    10021 cggccagccg gtcgcggagc ccctcctcga tgcggaagag gagggaggag ggccgcgcta
    10081 tcgccatgtc caggcggatc tcgcccctcg agtagtccag gaactcgctg gcgagccgct
    10141 ccaggcgcgc gacctcggcc aggctcgcct tgatcctggc gcgggccttc tccggcgcct
    10201 cgtcggcctg gatctccatg agctggagct ggcccttgag gatggagagg gggttccgga
    10261 tgtcgtggag gatgagggag gagaacttgc ccagggtgga gaggcgctcc gtccggaggc
    10321 gctcggcctg ggccttctcc aggtcggcgt aggcgcgctc gagctcggcg ttgcgccgac
    10381 ggaggtcgtc cacgaagacc tcgttggagc tccgcaccat gaaggagatg gaggtcatca
    10441 cggagagggc gatggaggag cgctccttga tgaggcgccg gaagtcgtcg cggtagaggt
    10501 agaggacgcg gacgggatcc ttcgcgacga cggtggcgct ccggggcagc tcgtccacca
    10561 gggccatctc cccgaagaag tggccggggc cgtggacggc gaggaggtcg ggcttggggt
    10621 cgtagaagtt cttccagacc tcgacccggc cctcgatgac gatgtagaag cggtcggccg
    10681 tcgatccctc gacgaagagg acgtcgccgg ccgcgtactc ctcctcggcg caggagccct
    10741 cgacgaggcg gatctcgtcg tcggccaggt ccttgaagaa atagacccgc ctaaggaact
    10801 cgaagtagtc cagctcttgc gccataagaa tcgggccctc cctttcatgg gggagggccc
    10861 gcctcgtgga ggctcgcctg gctaggcggt caccaggtgt cgctcatgtc gtcggggtcg
    10921 cggtcgtcct cggcgaagag gtcggtcacg gccctgaggt cgtcgaagta gaggtagtag
    10981 gtgccgaagg cctccatggg gtcgcactcg accttgaagc cgacgatctt gaggcccatg
    11041 tggctcgtgt agtggaagtt ccgctggacg atgccgctct gcccgtcggg gttctgggga
    11101 ggcaccgcga cggagagctt cttccagccc tggaagttga gcttgcccag ggggagctcg
    11161 aagcgctggc cgaagaagtc ctggaggacg atcttgagcg tgtggttgta gttgcggccg
    11221 acgacccaga cggagatcgt cttcgtgatc ccctccacgg ggatcgggcg cttggccacg
    11281 atcatgagct cgttgtagcc gcggcggtag aagtcggtgc gcacgcccag ggcgtacttg
    11341 tcggcgatct tggggtcgat gccgatgtag cgctcgtcgg ggatgggctc cgcggccttg
    11401 ccggcggggc cgccctcgaa gaggcgggcc tggatgtagc cgtcgtcggc ggcgatgtag
    11461 gcgttccaga agccggcggc ctcgaacttg tcgacggaga cttccttgag cttctgctgg
    11521 gcggtgtcgg cgccgatctt ggcggggtcg ggggcgccga tctccgcggg ggcggcggcg
    11581 ccctgcgcga aagcggaggc cgcgaaggcg gaggcgagga ccgcgacggc gagcaaggcc
    11641 ttggtggata tcttcattgt cgtcctccct tatttcttct cgcccgagcc ccaggcttcc
    11701 ttgatgacgt ccggcctctg gaggctgtcg ccgtcgaaga gcccttcgaa ggtgtcggtg
    11761 agcaccttga ggttgtcgaa gtagaacttc accgccttgt cgatgtcggg cgaattcggg
    11821 tcgttggtca ggatggcgac gttctcggtc ggcctggtcc agatgcggaa cttgacgagg
    11881 ctgaggctct cgcgcttggg aaggtacttc ttggactggg ggacgttgtc gggcatgttg
    11941 acccgcatgt tcttccagcc gatgaagttg agctcgccca tcggcaggat gtgcacgatc
    12001 cccttgtagt cgcggatgta ggcctcgatg tagtagttgt agttgccgga ccagacccac
    12061 atgtcgatgg ccttgacgcg gccgggcagg ggcagctcga cgggctcgta cttgacgttc
    12121 tcgccctcgc cggacttctt gccggggacc aggtcgaccc agttgtactc cttgcggtcg
    12181 tagagggtcg cgatgcccag ggccttgagg tccttgccct cgggattgga gccgtagagg
    12241 gcgaggggcc aggtcttcac gtagctttcc ctggggaagc cggcggtgga gaacttgctg
    12301 ccgatgagga accagggctc cttggcgggg tcatcgaagc tctggatgac caccgactcg
    12361 aggttgacgg tattctcgtc cgcgaaagcg cttccgacgc cgatgccgga caaaaggacg
    12421 aggaaaaggc aaaaagcccg aataacgctc tgtttcatcc ttgaaactcc tttatcggta
    12481 ccgcggacct gtgtcggcgg ttgcattcga ttatattttc ggacaatacc tttgtcaaat
    12541 acattcccct cttggaattc cctcgggaat tcgcgagcgg ggcccccggg cagtgcgccc
    12601 aacgcgcgtt gaagaatata aggaaggggc ccggggcggc cggaaggcgg ccgccggctc
    12661 gggcctcgag gccgggcggc ggcgtacggg ccccgcccta tgccctcagc ggccgcccgc
    12721 ggcgcggcgg aggagggcgg cgaggctcga ctctccagcg cggaagccct cggccgagcg
    12781 ggcccggccc aggagggcgg gcacgaggac gaggcgcgac acggtgtcgc ccgcgccgtc
    12841 ctgggcaggc cgcgccgcgc cgccttgcgg ggagcgcgac gcggcgagcc ccgcgccgcg
    12901 ggccaagtcc tccaccgccc gggccagggc ggcctcgtct ggctcgacgg agaagcaggc
    12961 ttcggggcag ccggagaggg gcgcggccgc gccgagggcg aggcccggcc tcgccgccct
    13021 ggcgcagaga cccgcgctcc cgggacccgc ggccacgaag aggatccgca ggtcggagcc
    13081 gaggagggcc tggagggccg cgtcggcttc ggcttccggc ccggcctggg gcgccgcggc
    13141 gccggccgga ggtgcgggcg gtaggggggg cgaaagcgga gggaagcccg cctcggcctc
    13201 gaaggcctcc gcgaaggcct cgcctaggac gcgcggccgc gcggccgact cggagaagag
    13261 gatggcgccc gtggcctcgg cggcgcccgc ggccctgagg gacgcgacgt aggcccccgc
    13321 cgccctgccg gcggcgcgat aggcgggctc gtagtcggtg gaggccgcga cgaggcccgc
    13381 tgggagcgga ccttgccatt cgggagcgac gatgagggct ccgtccaggg ggccttcggc
    13441 gccggctccg tccggcccga agcccgcggc cgagcccaag agggccgagg cgaggagggg
    13501 gcttgcgacc aggacgcgcg ggcccgaggc cccggacccg gcgacgccgg cggcccgggc
    13561 cgcctcgtag agcttcgagg ggccctcggc gaggcctacg gcggccggcc gcgaggcgga
    13621 gaggcggaag aggcggcggg cgagctccgg cctcgcggcg gcgaaggcct cgtccaggcc
    13681 gacgagcacc cgcctgcgcg gcccgcaggc ggggagaagg caggaaagca gggcgcagga
    13741 caggaggagg acggccgcct tgggggcgag gccgggggcc cggcccggaa ggggggggcg
    13801 ccgcgtcggg cggctgtgca tgggcccatc ctatacccgc ccgccgcctg cgggcaacgg
    13861 gcgcggcggc ccgcgaggcc ttgacgcccg gcccccggcc tacctaagat tggccttgat
    13921 agcatatctc tacaaggaga agccatgacc agctataagg agctcggact cgtcaacacc
    13981 gtcgagcttt tcaagaaagc cgtgaagggc ggctacgcga tccccgccta caacttcaac
    14041 aacatggagc agctccaggc catcgtccag gcctgcgtgg agac
//
LOCUS       DAOOSJ010000034        13919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2939_length_13919_cov_5.783035, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000034 DAOOSJ010000000
VERSION     DAOOSJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_13919_cov_5.783035"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..810
                     /locus_tag="PLG14_03025"
     CDS             4..810
                     /locus_tag="PLG14_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOX48133.1"
                     /translation="MDARSRAKAVSADAKRAAALFASVLALAGCATRAGGAGSSLAGP
                     SGGLPSGRLPAGAVLVDSGKPGPLLLVVAGMHGDEPSGPLAARILASFGADAALPGQS
                     WAIGRGALLVLPEANPEACSAGLRESPPKTGLPAEDLNRLFPGHRRGGEGPARAAEIF
                     AAALGADLSIDLHESGWAWTEADSPSLVLSPAAAPFALELLEALDGKGAPFAFTGGAP
                     YGSLVWALGAKGRAAIGVEAPARLSEAERIALHLAVVRESATLLGMTDRP"
     gene            807..2039
                     /gene="pgsB"
                     /locus_tag="PLG14_03030"
     CDS             807..2039
                     /gene="pgsB"
                     /locus_tag="PLG14_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOX48134.1"
                     /translation="MRPLELAALGAALAYVAFLFLERALLTRARKSLRLVAHVNGTRG
                     KSETTRLLAAALRASGFTVLAKTTGTEPRTILPDGRERRLFRAGAANVREQRDLVLEA
                     ARRGADAVVAECMAVSPEAQRASSAFLSPDILVVTNSRRDHRGELGSPEEAAAVFAEG
                     IPKGGSVVTADPSLYPLLAEAAASLGARAILAEPMGEAGSIFPDNAGAALAAALLAGA
                     ERESALAGMRAHLPDPGAFALRRLARKGGGSILVADALAANDPDSTAILWERSLKVLA
                     RIRPPKPGARRILLLANRADRPDRSLAFAEFTAQKAADFDALFLLGPLPMRAARRLRA
                     ARKGAARTGAARTGAGLPDRGASPSSVPRLVRFWRGSSVLAALRMEPEDTFVLAAGNW
                     KGFGPLLSMAAPPLEDAT"
     gene            2036..2467
                     /gene="pgsC"
                     /locus_tag="PLG14_03035"
     CDS             2036..2467
                     /gene="pgsC"
                     /locus_tag="PLG14_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOX48135.1"
                     /translation="MNSEYVLVIGAVTSLFMTEFFGVSPGGLLVPGYVALFFDSPLRL
                     LATIVDAGLALVAARLIARGAILFGRRRYAVFLLAGFVARFILERALPGLAPEAPVLA
                     AIGWLVPGIIASEADRQGPARTLLALAGAALFVRLLWAALP"
     gene            2350..3654
                     /gene="pgsW"
                     /locus_tag="PLG14_03040"
     CDS             2350..3654
                     /gene="pgsW"
                     /locus_tag="PLG14_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOX48136.1"
                     /translation="MARPRDHRLGSGQAGSREDSPGLGRRGVVRPAALGGPALSDPRL
                     PKPTAASAAPPARGASRATHPARGQGPERLVALVAFVLVLACWYCVLLAIGGGSDPAG
                     RAAEKRAAEAMEGAIAAIRVEAESRGIALDPGADPNRTGLVGLSWTGLTTTLGSLPAK
                     RTGAQPDAAALLARLLLEAGVGPGDRVAVDSSGSFPGFAVAALVAVRSLGAVPVAVIS
                     VGSSTWGANRPEFALPDMLAALESRGILPRGAAAVSAGGGDDSGGGMDAEALGGVLDR
                     SRGRGAALIAEPSLAQDVAARLRVFEAEGPIVALVSIGGNYSQAGAGEALTGRSGLLR
                     SGDFPPGGPKGEGLIQACLRAGLPVIRILDVKDLSARTGLAYDPVPWPAPGSSALWRG
                     RSPSRLLVAAGPVAAIAVSAVVLLAAKRRVLRARGDPGRLPR"
     gene            complement(3678..>4978)
                     /locus_tag="PLG14_03045"
     CDS             complement(3678..>4978)
                     /locus_tag="PLG14_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX48137.1"
                     /translation="ALRPAAEALAGLLSPAARLLGLYWAEGGHGVAVGAALAAALPFI
                     LVLGAAFAKGRLFCGGLCPVGSLLGLLNRAAVLRVRLEPGACVSCGACERICKARCLD
                     AAGKTLDADRCVSCFSCLGVCPTGALAYGPGPRRGAGPGKGGPAPDGKKPEGIGPQEP
                     SPAEPGSMGQWPEEPGQAGLGPSRRSFLKKAASGGAAAALALLPAPLSRAAAPRGSGG
                     EGLEAAAPPGAGSLERFLARCTACGLCVSKCPSKVLRPSVARYGARGFLAPYLDYDRS
                     YCQYECAACLELCPTGALERMPIGRKKLVQMGTAALVREKCIVVEKRTACGACAEHCP
                     TGAVRMVARGPGLPEPLFDEAICVGCGACHRICPALPDKAITVAGKSPQGLALAPRKD
                     LFAAETAGGRKGGAGQSGDVSERAGGAEAEDGAGAEEFPF"
     assembly_gap    4979..5078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5079..5578)
                     /locus_tag="PLG14_03050"
     CDS             complement(<5079..5578)
                     /locus_tag="PLG14_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX48138.1"
                     /translation="MRRRIHPLAAARIAASGLFAALFALAFLGPAAATGGLFGLVAGT
                     QLFPAAARLLAGGALASAACALALAASTLLFGRLYCSLLCPLGAAQDAAFALGRRLRE
                     YRLGYARGPGLLRAASFAAAALALALGAAPAASFLDPYSLSGRFLEYALRPAAEALAG
                     LLSPAA"
     gene            complement(5591..6562)
                     /locus_tag="PLG14_03055"
     CDS             complement(5591..6562)
                     /locus_tag="PLG14_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOX48139.1"
                     /translation="MDRREFLEAAAKYGLRAAAALLAGGAYPLFAQGAQAAPPPTTSA
                     AQPPRAAAGARLPDLVAVKGGEPEALFDAGIAALGGMRAFVKAGQTVVVKPNIGWDQP
                     PEAAANTNPRLVKRVVEHCLDAGARKVYVFDNSCDAWTLCYKSSMIERYAREAGAEVV
                     PANAAGYYQEVGFPGAAVLKRAKVHELLVECDVFINVPVLKHHSGAGMTAAMKNLMGA
                     VWDRQFFHRNDLDRCIAEACLYKKPSLNVVDAYKVMKTGGPRGYASSVYETARFQILS
                     ADIVAADAAAARTLGFEPSRFPYVGLAAAQGSGQADLSRLDIRRIVL"
     gene            complement(6597..7715)
                     /gene="ilvE"
                     /locus_tag="PLG14_03060"
     CDS             complement(6597..7715)
                     /gene="ilvE"
                     /locus_tag="PLG14_03060"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOX48140.1"
                     /translation="MMESGGAGRVGQVTGFEAMMVPGPPPADPKTIDWETLGFAPLVT
                     AKVSGSLCGPGGVFEPPKVVPSGLLALPPQATALNYGQALFEGLKARRGVDGRARIFR
                     LADNARRMRAGAERFMLAAPGEALFAEAVLACARGNLDYLPPCGKGSLYLRPLLLGTG
                     RTLGLGPSPETLFLVYANPVGLYFKGLASISILACEDHQRAAERGTGGVKAAGNYAPC
                     FRPTAAAKTAGFADLLYLDHEGKRIEEVGSANLFAVKDGRLLVADSASILPGITRDSV
                     ETLAREALGMETERGELGLDRVLGRGAWEAEGPADEVFCTGTAAIVSPIGRITHRGRE
                     TVFGSGEPGPVTRRLHDELDGIQTGRLPDARGWTTFVD"
     gene            complement(7846..8550)
                     /locus_tag="PLG14_03065"
     CDS             complement(7846..8550)
                     /locus_tag="PLG14_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOX48141.1"
                     /translation="MQDYYETLELGRAAGPDEIKANYRRLAMKWHPDRNPGSAAAEER
                     FKSISEAYSVLSDASKRREYDAYLGAGGSFAGTGGARADGTWTGGSWTGGASAEGARQ
                     GSGFRWAGGAFTREEASFMFMREMYALATELTLQNVAWQDIARELVRRGCPEDVAADI
                     ARRMEAGRKAMIRERAKPYFVRSALSGFFGLGLFAAFAGVGFGLLGFLGLAMFLSGAY
                     NLVRAFYHLATGKAPV"
     gene            complement(8608..8949)
                     /locus_tag="PLG14_03070"
     CDS             complement(8608..8949)
                     /locus_tag="PLG14_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48142.1"
                     /translation="MKKSFPVLLALAAALALGGCELFTSTVEYNVSGGSGFMAIRYQD
                     KGGELVETTVSSPWSTSFDLWSKDRPFLAFLQVTSGGAGATLYISVDGSVVASASLAA
                     SPTGQVLYIVE"
     gene            9057..9473
                     /locus_tag="PLG14_03075"
     CDS             9057..9473
                     /locus_tag="PLG14_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48143.1"
                     /translation="MPFSCSNEVLVSGGDPADRRRALAMLAAADWGLEPVAAEDAEAV
                     SGLTDRLRFGSLDAIPEDEIRNIAPQFPDLDFLLLYLSIDGEFFGFLRIRGTDAVEES
                     ADFPDGDGPEIERIEEAQPLGQVRRFYAGNPAILLP"
     gene            9546..10667
                     /locus_tag="PLG14_03080"
     CDS             9546..10667
                     /locus_tag="PLG14_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOX48144.1"
                     /translation="MQSKRAKAFSLPFLLAFLLSAAALPASSLRPERLEFSEVWAYLL
                     AGEERFLDPALPISDLGYFGAGLSPFGKLVGVPKRESLKGYPGRVHLVVAEVGNQALT
                     HFCLDPAYPLRDALIADIVAAAGPFDGVQIDFEAVSQADYDNFFAFLGLLKRGLGAKT
                     LSVALPARVSEKSDYFGYERIGRLADRVVVMAYDEHWSTSAPGPVASLEWCGKVAAYA
                     LGKVPPERLVMGAPFYGRAWADKALSRAYKYSSLSELLAEKRIEDVRREGGIPFVEYV
                     EAVTVRLFFDDAASLHSRLSMYRKAEVRSVAFWRLGQEDPAVWASIAAAPSGAAPTGA
                     TTPGAAPSGTADGGLAGIQAFAEAGPATRLYELRTAPPL"
     assembly_gap    10728..10827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10923..11792)
                     /locus_tag="PLG14_03085"
     CDS             complement(10923..11792)
                     /locus_tag="PLG14_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOX48145.1"
                     /translation="MLAKVFLEIASGCNLSCSFCPPTRRKAEFLDRGRFELFLDRLSG
                     SGDHLYFHLKGEPLLHPGLADFLAAAGGRGFAVTLTTNGTLVGKRAEALLGAANLRKL
                     SVSLHSHSGAPDPEEYWRGVEAFLDAHRLRPSFPVSLRLWNRSAGRLPPGTARLWELL
                     RARYPALGDWDPDAVLPAGTRLDHRVYLNPAEEFAWPDLSRPEEGTRGFCHALRNQAG
                     VLVDGTVVPCCLDGEGVMALGNLRDAPLGEILAAPRARAIREGFSRRELVEPLCRTCG
                     YRKRFDSGGLSKA"
     gene            complement(11782..12918)
                     /locus_tag="PLG14_03090"
     CDS             complement(11782..12918)
                     /locus_tag="PLG14_03090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOX48146.1"
                     /translation="MPLLALALLASAWGDTLPLSEAMAALCLTLTLEGALVARAHHGA
                     DRRARKNRLLGELASEVNRAILLNEDDQKIFSTVLDYAFRILEKVNLGSVLAFDESGE
                     LVIVASHGFSEDFVRSFRLRLEDTFQFRQSGGKISEAMIIDPEIIKGFADKLDSTSKV
                     YKSIISAPLFVEGKLYGFLNVDSERARCFGAEDLELLKNFASQIEVCLLARQRYRSSL
                     AESRVDGLTGFLSRSYFEELAEHSVERAARYGQGFSLGMFDVDGLKAVNDGLGHQAGD
                     LVLSAVAAAIRRAARKSDVTGRYGGDEFIAICFAADAKTMEERTARILAELRASPVVF
                     GDRGLAASFSYGFANFPQDAGTLSGLVAAADARLYAMKAKRRAG"
     gene            complement(13042..13467)
                     /locus_tag="PLG14_03095"
     CDS             complement(13042..13467)
                     /locus_tag="PLG14_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HOX48147.1"
                     /translation="MNENYFMEETPRPEPAAVQAALGGMYPWYEGILAAAEGFERDWK
                     HYGRKYGWKLKLHDGDKALAEITVTPAGVLVGVAAREAEIEELREEAEKALGGPLAEA
                     LAAGKAKGSWGLRLFVADEAAYGKARALLEAVAEIRRRD"
     gene            13660..>13919
                     /locus_tag="PLG14_03100"
     CDS             13660..>13919
                     /locus_tag="PLG14_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX48148.1"
                     /translation="MPFSDLPLSSGLRRAITGLGAVEPFPVQAAAIPLVLAGRDLAVS
                     AKTGSGKTLAFAAPILDRLALAPARKGGLVRALVLVPSRELAA"
BASE COUNT         1856 a         4889 c         4967 g         2007 t
ORIGIN      
        1 gccatggatg cgaggagtcg cgccaaggcc gtctccgcgg acgcgaagag ggccgcggcc
       61 ctcttcgcgt ccgtcctggc cttggcggga tgcgcgaccc gggcgggcgg tgccggatcg
      121 agtctcgccg ggccgtcggg cggcctgccg tcgggccgtc tgccggcggg cgcagttctc
      181 gtcgattcag gcaagcccgg gccgcttctc ctcgtcgtag cgggcatgca tggcgacgaa
      241 ccttccggcc ccctggcggc gcggatcctg gcgtcgttcg gcgcggacgc ggcccttccc
      301 ggccagtcct gggccatcgg ccggggagcc ttgctcgtcc tgccggaggc caacccggaa
      361 gcctgctccg ccggcctccg cgagtcgccc cccaagactg gccttccggc agaggacctg
      421 aaccgcctct tccccggcca ccgccgcggc ggcgagggac cggcacgcgc ggccgagatc
      481 ttcgccgcgg ctctaggggc cgatctgagc atcgaccttc acgagtcggg atgggcctgg
      541 accgaggcgg actccccttc tctggtgctt tctcccgccg ccgcgccttt cgcccttgag
      601 ctcctcgagg ctctggacgg gaaaggcgcg cccttcgcct tcactggcgg cgctccctac
      661 ggcagcctcg tatgggccct cggggccaag ggccgggccg cgatcggcgt cgaggcgccg
      721 gcccggctct ccgaagccga gagaatcgcg ctccatctcg ccgtggtccg ggagtcggca
      781 acgcttctcg ggatgacgga ccggccatga ggccgctcga gctcgcggcc ctgggagccg
      841 ccctggcgta tgtcgcgttc ctgttcctgg agcgggcgtt gctcacccgc gccaggaagt
      901 ccctgcgcct tgtcgcgcac gtgaacggca ccaggggcaa gtccgagacg acaaggcttc
      961 tggcggcggc cttgcgggct tccggcttca cggtgctcgc gaagacgacg gggaccgagc
     1021 cgaggaccat cctgccagac ggacgggagc gccggctgtt ccgggccggg gcggccaatg
     1081 tccgggagca gcgcgacctt gtcctggagg cggcccgtcg cggcgccgac gcggtcgtcg
     1141 cggagtgcat ggccgtctcg ccggaggccc agcgcgcctc ttccgccttc ctttctcccg
     1201 atatcctcgt cgtgaccaac tcccggcgcg accaccgggg cgagctcggc tccccggagg
     1261 aggccgccgc cgtcttcgcc gaaggcattc cgaaaggagg ctccgtcgtg acggccgatc
     1321 cctcgctcta tcctctcctg gccgaagccg ccgcctccct gggcgcccgg gccattctcg
     1381 cggagccgat gggagaggcg ggaagcatct ttcccgacaa cgcgggcgct gccctcgcgg
     1441 cggccctcct cgcgggagcc gaaagggaga gcgccctcgc cggcatgcgg gcccatcttc
     1501 ccgatccagg ggccttcgcg ctccggcgcc tggcgcggaa aggcggcgga tccatcctcg
     1561 tcgccgacgc gctcgcggcc aacgatcccg attccaccgc gatcctctgg gaacgctccc
     1621 tgaaggtctt ggcgaggata cgtcccccga agcccggcgc aaggcgcatc ctcctcctcg
     1681 cgaacagggc ggacaggccc gaccggagtc tcgctttcgc cgagttcacg gcccagaagg
     1741 ctgccgactt cgatgccctg ttcctgcttg ggcctttgcc tatgcgcgcg gcgcgccgac
     1801 tccgcgccgc ccgcaaaggc gccgcccgca caggcgccgc ccgcacaggc gccggcttgc
     1861 ccgaccgcgg agcgtctcct tcctcggttc cgcgcctggt acgcttctgg cgaggctcct
     1921 ccgtcctcgc agctctccgc atggagccgg aagacacttt tgtcctcgcg gcgggcaact
     1981 ggaagggctt cggccccctc ctgtccatgg ccgccccgcc cttggaggat gcgacatgaa
     2041 ttcggaatac gtcctcgtca tcggggcggt gacaagcctc ttcatgacgg aattcttcgg
     2101 cgtctcgccc ggaggtctcc tcgtgccagg ctatgtcgcg ctcttcttcg attcgccgct
     2161 tcgcctcctc gcgaccatcg tcgacgccgg cctggccctc gtcgctgcca gactcatcgc
     2221 gagaggagcc atcctcttcg ggcggaggcg ctacgcggtg ttcctgctcg cgggcttcgt
     2281 cgcgcggttc atcctggagc gcgcgcttcc cggcctcgcg cccgaagcgc cggtccttgc
     2341 cgccatcgga tggctcgtcc ccgggatcat cgcctcggaa gcggacaggc agggtcccgc
     2401 gaggactctc ctggccttgg ccggcgcggc gttgttcgtc cggctgctct gggcggccct
     2461 gccttgagcg atccgcgcct tccaaaaccc accgcggcga gcgccgcgcc tcccgcccga
     2521 ggcgcgagcc gcgccacgca tccggcccgc ggccaaggac cggaaaggct cgtcgccctg
     2581 gtcgccttcg tcctagtcct tgcctgctgg tattgcgtcc tgctggcgat cggcggggga
     2641 agcgatcccg cgggccgggc cgcggagaag cgcgccgccg aggccatgga aggcgccatc
     2701 gccgccatcc gggtcgaagc tgaatcgcgg ggaatcgccc tcgatccggg cgccgatccc
     2761 aaccgcacgg gcctggtcgg cttgtcttgg acggggctta cgaccacgct ggggagtctc
     2821 cccgccaagc gcacgggtgc gcagcccgac gcggccgccc tgctggcaag gcttctgctg
     2881 gaagcgggcg tgggtccggg agaccgtgtg gccgtcgaca gttcgggatc ctttccgggc
     2941 ttcgcggtcg ccgccctcgt cgcggtccgg tcgctcggcg cggtcccggt ggccgtcatc
     3001 tccgtcggct cctcgacctg gggggccaat cgtcccgagt tcgccctgcc cgacatgctc
     3061 gcggccctgg agagccgcgg catcctcccg cgcggggccg cggccgtctc ggcgggcggc
     3121 ggggacgatt caggcggcgg catggacgcc gaagccttgg gcggagtcct tgaccggtcg
     3181 aggggccgcg gtgccgcgct gatcgcggag cccagcctgg cccaggacgt ggcggcgagg
     3241 ctccgcgtct tcgaggcaga gggacctatc gtagcgctcg tctccatcgg cgggaactac
     3301 agccaggcgg gagccgggga agccctgacg ggccgctcgg gacttctccg ttccggggat
     3361 tttcccccag ggggaccgaa aggcgagggg ctgatccaag cctgtctccg cgcgggcctg
     3421 cccgtcatca ggatcctgga cgtgaaagac ctgtccgcgc gcacgggcct cgcctatgat
     3481 cccgtgccct ggcctgcccc cggaagctcg gctctgtgga ggggccggag cccttcgcgc
     3541 ctccttgtcg cggccgggcc cgtcgccgcc atcgccgtct cggcggtcgt cctgcttgcc
     3601 gccaagagga gagtacttcg agcgcggggc gatccagggc gtctccctcg atagaggccc
     3661 gagtcccaat cccggcccta gaaggggaac tcctcggccc ccgcgccgtc ctccgcctcg
     3721 gctccgcccg cccgttcgga tacgtcgccg ctctgtcccg cgccgccctt gcgccctccg
     3781 gccgtctccg cggcgaaaag gtccttgcgc ggcgccaggg ccaggccctg cgggctcttc
     3841 ccggccacgg tgatggcctt gtcgggcagg gccgggcaga tccggtggca ggcgccgcag
     3901 ccgacgcaga tcgcctcgtc gaagaggggc tcgggaaggc cggggccccg ggcaaccatg
     3961 cgcacggcgc ccgtcggaca gtgctcggcg caggcgccgc aggccgtcct cttctccacg
     4021 acgatgcact tctcccgaac gagggccgcc gttcccatct ggacgagctt cttgcgcccg
     4081 atcggcatgc gctcgagcgc tcccgtgggg cagagctcca ggcaggcggc gcactcgtac
     4141 tggcagtagg agcggtcgta gtcgaggtag ggcgcgagga agcccctggc gccgtagcgc
     4201 gcgacggagg gacgcaggac cttggagggg cacttggaga cgcagagccc gcaggccgtg
     4261 cagcgggcga ggaagcgctc cagggagccg gctccgggcg gagcggccgc ctcgagcccc
     4321 tctcctccgg agccgcgagg ggccgcggct cgggagaggg gcgcggggag gagggcgagg
     4381 gcggccgctg cgccgccaga ggccgccttc ttgaggaagc tccttcgcga ggggccaagg
     4441 ccggcctggc ccggctcctc cggccactgg cccatcgagc ccggttcagc cgggctcggc
     4501 tcctgcgggc cgatgccttc tggtttcttt ccatcggggg cggggccgcc cttgccgggc
     4561 ccggcgccgc gccgcggccc cgggccatag gccagggctc ccgtcgggca gacgccgagg
     4621 caggagaagc aggagacgca gcggtcggcg tccaaggtct tgcccgcggc gtcgagacag
     4681 cgcgccttgc agatccgctc gcaggcgccg caggagacgc aggccccggg ctcgagccgg
     4741 acccgcagga ccgcggccct gttgaggagg ccaaggaggc ttcccacggg gcagaggccg
     4801 ccgcagaaga gcctgccctt cgcgaaggcc gcgcccagca cgaggatgaa gggcagggcc
     4861 gcggccaggg ccgccccgac ggcgacgcca tggccgccct cggcccagta gaggccgagg
     4921 agtctcgcgg cgggggagag gaggccggcg agggcctcgg ccgcggggcg cagggcgtnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct cgcggcgggg gagaggaggc
     5101 cggcgagggc ctcggccgcg gggcgcaggg cgtactcgag gaagcggccc gagaggctgt
     5161 agggatcgag gaaggaggcc gccggcgccg cgcccagggc cagggccagg gccgcggcgg
     5221 cgaaggaggc ggcgcggagg aggccgggcc cccgcgcgta gccgaggcgg tattcccgga
     5281 gcctccgccc cagggcgaag gccgcgtcct gggccgcgcc cagggggcag aggagggagc
     5341 agtagagcct accgaagagg agggtcgagg cggcgagggc cagggcgcag gcggccgagg
     5401 cgagggctcc ccccgcgagg agccgcgcgg cggccgggaa gagctgcgtc ccggcgacga
     5461 ggccgaagag gccgcccgtg gccgcggccg gccccaggaa ggccagggcg aaaagggcgg
     5521 cgaagaggcc ggaggcggcg atcctcgccg ccgcgagggg atggatccgg cgccgcatgc
     5581 cctgtcgccc ctagaggacg atcctccgta tgtcgagcct ggagaggtcg gcctggccgc
     5641 tcccctgcgc ggccgcgagg ccgacgtagg ggaagcggga gggctcgaag ccgagggtcc
     5701 tggccgccgc ggcgtcggcg gcgacgatgt ccgcggagag gatctggaac ctggccgtct
     5761 cgtagaccga gctcgcgtag cccctcggcc cgccggtctt catcaccttg taggcgtcca
     5821 cgacgttgag cgacggcttc ttgtagaggc aggcctcggc tatgcagcgg tccaggtcgt
     5881 tgcggtggaa gaactgccgg tcccagaccg cgcccatgag gttcttcatc gccgccgtca
     5941 tgccggcccc cgagtggtgc ttgaggacgg gcacgtttat gaagacgtcg cactcgacga
     6001 ggagctcgtg gaccttcgcc cgcttgagga ccgcggcgcc ggggaagcct acctcctggt
     6061 agtagcccgc cgcgttggcc ggtaccacct ctgcccccgc ctccctcgcg tagcgctcga
     6121 tcatggagga cttgtagcag agggtccagg cgtcgcagct gttgtcgaag acgtagacct
     6181 tgcgggcccc cgcgtccagg cagtgctcca ctacgcgctt cacgagcctg gggttggtgt
     6241 tggccgcggc ctcgggcggc tggtcccagc cgatgttggg cttcacgacg accgtctgcc
     6301 ccgccttcac gaaggccctc atgcctccga gcgcggcgat ccccgcgtcg aagagggcct
     6361 cgggctcgcc gcccttgacc gcgacgaggt cgggtaggcg cgcccccgcg gcggcgcggg
     6421 gcggctgcgc ggccgaggtg gtgggaggcg gcgcggcctg ggctccctgc gcgaagaggg
     6481 gataggcgcc gcccgccagc agggccgcgg ccgcgcggag gccgtacttg gccgccgcct
     6541 cgaggaactc cctcctgtcc atgcctcctc ctcgaaggcc ccccgccggg gagtcttcag
     6601 tcgacgaagg tggtccagcc ccgcgcgtcc gggaggcgcc cggtctggat cccgtccagc
     6661 tcgtcgtgca ggcgccgcgt gacgggcccc ggctccccgg agccgaagac cgtctcgcgg
     6721 ccgcggtgcg ttatgcggcc gatcggcgac acgatggccg ccgtccccgt gcagaagacc
     6781 tcgtcggcgg gcccctcggc ctcccacgcg cctcggccca ggacgcggtc caggccgagc
     6841 tcgccgcgtt ccgtctccat gccgagcgcc tcgcgggcca gcgtctccac ggagtcgcgg
     6901 gtgatcccgg ggaggatgga ggccgagtcc gcgacgagga gcctgccgtc cttcacggcg
     6961 aagaggttgg cgctccccac ctcctcgatc cgcttgccct cgtggtccag gtagaggagg
     7021 tcggcgaagc ccgccgtctt ggcggcggcc gtgggcctga agcagggcgc gtagttgccc
     7081 gccgccttca ctccccccgt gccgcgctcg gcggcgcgtt ggtggtcctc gcaggcgagg
     7141 atggagatcg aggcgaggcc cttgaagtag aggccgacgg gattggcgta gacgaggaag
     7201 agggtttcgg gcgagggacc gaggccgagg gtcctccccg tcccgaggag gaggggcctg
     7261 aggtagagcg agcccttgcc gcagggaggg aggtagtcga ggttgccccg cgcgcaggcc
     7321 agcacggcct ccgcgaagag cgcttcgccc ggggccgcga gcatgaagcg ctcggccccc
     7381 gccctcatgc gcctcgcgtt gtcggccagg cggaagatcc tcgccctgcc gtcgaccccg
     7441 cgccgggcct tgaggccctc gaagagggcc tggccgtaat tgagggccgt cgcctgcgga
     7501 gggagggcaa ggaggccgct cgggaccacc ttggggggct cgaagacgcc gccagggccg
     7561 cagagggagc ccgagacctt cgcggtgacg aggggcgcga agcccagggt ctcccagtcg
     7621 atcgtcttcg ggtccgcggg cggggggccg gggaccatca tcgcctcgaa gcccgtgacc
     7681 tggccgaccc ggcccgcgcc gcctgattcc atcatcgcct gctccttggc aacccgcctg
     7741 agtggcctgg ttgtttcgat gcatagtatt atggccggga aggcggcgga agccctttcg
     7801 gacgcgggcg cggacctggg atcagccgcc ggacggcgtt cgtctctaga ccggggcctt
     7861 gcccgtcgcc aggtggtaga aggcccggac caggttgtag gcgccgctca ggaacatggc
     7921 gaggcccagg aagccgagga ggccgaagcc gacgcccgcg aaggccgcga agaggccgag
     7981 gccgaagaag cccgagaggg ccgagcggac gaagtagggc ttggcccgct cgcggatcat
     8041 cgccttgcgc cccgcctcca tcctgcgggc gatgtcggcc gcgacatcct ccgggcagcc
     8101 ccggcgtacg agctcgcggg ctatgtcctg ccaggccacg ttctgcagcg tgagctcggt
     8161 cgcgagggca tacatctccc gcatgaacat gaaggaggct tcttcccggg tgaaggcgcc
     8221 gccggcccag cggaaaccgc tgccttgtcg cgcgccctcg gcgcttgcgc cgcctgtcca
     8281 cgagccgcca gtccatgtgc cgtccgcgcg cgctcctccc gtgcccgcaa aggagccgcc
     8341 agctccgaga taggcgtcat actcgcgccg cttggacgcg tcggagagga ccgagtaggc
     8401 ctcggatatg ctcttgaagc gctcctcggc cgcggccgag cccggattcc gatcgggatg
     8461 ccacttcatc gccaggcggc ggtaattggc cttgatctcg tcggggcccg cggctcggcc
     8521 gagctcgagc gtctcgtagt agtcctgcat gcccgccaag atactgaaaa aggggcatct
     8581 cggcgagtgg aaccgccagc gtcccgccta ttcgacgatg tagagcacct gtcctgtcgg
     8641 actggccgcc aagctggcgg aggcgaccac ggagccgtcg acggagatgt agagcgtcgc
     8701 tccagcgccg ccggaggtga cctggaggaa ggccaggaag ggacggtcct tgctccagag
     8761 gtcgaagctc gtcgaccagg gagaagagac cgttgtctcc acgagttcgc cgcccttgtc
     8821 ttgatagcgg atggccatga agccggagcc tccgctcacg ttgtactcca ccgtgctcgt
     8881 gaagagttcg cagccgccca gggccagggc ggccgcaagg gctagcagga cgggaaagga
     8941 ctttttcatc gcatccctcc ttcccgaaag catatcccgc cccgcgcccg atcgcaggcc
     9001 gctggccctt ggcccgcgcg ggatgctatc cttgaggcga caagggggac agctccatgc
     9061 cgttcagctg ctcgaacgaa gtcctggtct caggcggcga cccggccgac cggaggcggg
     9121 cgctcgccat gctcgccgcg gccgactggg gcctcgagcc cgtcgcggcg gaggacgccg
     9181 aggccgtctc cggcctgacg gaccggctcc gcttcgggag cctcgacgcc atccccgagg
     9241 acgagatccg gaacatcgct ccccagtttc ccgacctcga tttcctcctt ctctatctgt
     9301 ccatcgacgg ggaattcttc ggcttcctgc gcatccgcgg caccgacgcg gtcgaggaga
     9361 gcgcggactt ccccgacggc gacggtcccg agatcgagcg gatcgaggag gcccagcccc
     9421 tggggcaggt gcgcaggttc tacgcgggga accccgccat cctcctgccc tgaagcccgg
     9481 ccggcgcgga ggcgatagcg tctggagtct cggctcgcct cgccgatact gaaagggagg
     9541 tgacgatgca gtcgaaacgc gccaaggctt tttcgcttcc ctttcttctc gcctttctcc
     9601 tctccgccgc cgcccttccc gcgtcgagcc tccggcctga gcgccttgaa ttctcggagg
     9661 tctgggccta tctcctggcg ggcgaggagc gcttcctcga tcccgccctc ccgatcagcg
     9721 acctgggcta cttcggcgcg gggctgagcc ctttcgggaa gctcgtcgga gtgcccaagc
     9781 gggagagcct caagggctac cccggcaggg tccacctcgt cgtcgccgag gtggggaacc
     9841 aggccctcac gcatttctgc ctcgaccccg cgtaccccct ccgcgacgcc ctgatcgccg
     9901 acatagtcgc cgcggcggga cccttcgacg gcgtgcagat cgatttcgag gcggtgagcc
     9961 aggcggacta cgacaacttc ttcgccttcc tcggcctctt gaagcggggc ctcggcgcga
    10021 agaccctgag cgtggccctg cccgccaggg tgagcgagaa gtcggactac ttcggctacg
    10081 agcgcatcgg caggctcgcc gaccgcgtcg tcgtgatggc ctacgacgag cactggagca
    10141 cgagcgcccc cggtcccgtc gcctccctcg agtggtgcgg caaggtcgcc gcctacgccc
    10201 tcggcaaggt cccgcccgag cggctcgtca tgggcgcccc cttctacggc cgcgcctggg
    10261 ccgacaaggc cctctcccgc gcctacaagt actcgagcct ctcggagctc ctcgccgaga
    10321 agcgcatcga ggacgtgcgg cgcgagggcg gcattccctt cgtcgagtac gtcgaggcgg
    10381 tgaccgtcag gctcttcttc gacgacgcgg cctcgctcca ttcgcgcctt tcgatgtata
    10441 ggaaggccga ggtcaggagc gtcgccttct ggcgcctcgg acaggaggat cccgcggtct
    10501 gggcgagcat cgcggccgcg ccttcaggcg cggcgcccac cggcgcgacg actccgggcg
    10561 ccgcgccctc cgggacggcc gacggcgggc tcgccggcat ccaggccttc gccgaggccg
    10621 gtcccgcaac ccgactctac gagctccgca ccgcgccgcc gctctaggat cctgtccggg
    10681 acccctgcgc gcgcggcatg gaaccgcggg cgcgacgcga tcggcgcnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnntgt ccgggacccc tgcgcgcgcg gcatggaacc
    10861 gcgggcgcga cgcgatcggc gctggcgacc gagcttgccg gggcagacgg cggattctag
    10921 gcctaggctt tggacaaacc gcctgagtcg aacctcttcc tgtacccgca ggtcctgcac
    10981 agcggctcga cgagttcgcg gcgggagaag ccctcgcgga tcgcgcgggc gcgcggcgcg
    11041 gcgaggattt cgccaagggg cgcgtcgcgc aggttgccca gggccatgac gccctcgcca
    11101 tccaggcagc agggtacgac ggtcccgtcc acgaggacgc cggcctggtt ccgcagggcg
    11161 tggcagaagc ccctcgtccc ctcctcgggg cgcgagaggt cgggccaggc gaattcctcc
    11221 gcggggttca agtagacgcg gtggtcgagc ctggtgcccg ccgggaggac ggcgtccggg
    11281 tcccagtccc cgagggcggg atagcgcgcg cgaaggagct cccagagccg cgccgtcccg
    11341 ggcggcaggc ggcccgcgct ccggttccag agcctgaggc tcacggggaa ggagggccgc
    11401 aggcggtggg cgtccaggaa ggcctcgacg ccgcgccagt attcctcggg atcaggcgcg
    11461 cccgagtggc tgtggaggga gacgctcagc ttgcgcaggt tcgccgcgcc gaggagggct
    11521 tctgcccgct tccccacgag ggtgccgttc gtcgtgaggg tcacggcgaa gccccgcccg
    11581 cccgctgcgg cgaggaagtc cgcgaggccg ggatggagga ggggctcgcc cttcaggtgg
    11641 aagtagaggt ggtcgcccga gcctgagagc ctgtccagga agagctcgaa gcggccgcgg
    11701 tccaggaact cggctttccg ccgggtcggc gggcagaagg agcaggagag gttgcagccg
    11761 ctcgcgatct cgaggaagac cttagccagc acgccgcttc gccttcatcg cgtagagcct
    11821 cgcgtccgcg gcggcgacga ggccgctcag ggtccccgcg tcctggggga agttcgcgaa
    11881 gccgtagctg aaggaggcgg cgagcccgcg gtcgccgaag accaccgggc tggcccggag
    11941 ctccgcgagg atgcgggccg tgcgctcctc catggtcttc gcgtccgcgg cgaagcagat
    12001 cgcgatgaac tcgtcgccgc cgtacctgcc cgtaacgtcc gatttcctgg ccgcgcgcct
    12061 gatggcggcg gcgaccgcgg agaggaccag gtcgcccgcc tggtggccca ggccgtcgtt
    12121 cacggccttg aggccgtcga cgtcgaacat gcccagggag aagccctggc cgtagcgggc
    12181 ggcgcgctcg acggaatgct cggcgagctc ctcgaaatag gagcgggaga ggaagcccgt
    12241 gagcccgtcg acgcgggact ccgcgaggga ggagcggtag cgctggcggg cgaggaggca
    12301 gacctcgatc tgggacgcga agttcttgag gagctccagg tcctcggccc cgaaacagcg
    12361 ggcccgctcc gagtccacgt tgaggaagcc gtagagcttc ccctccacga agaggggcgc
    12421 ggagatgatg gacttgtaga ccttgctcgt cgagtcgagc ttgtcggcga agcccttgat
    12481 gatctcggga tcgatgatca tcgcctcgga gatcttcccc cccgactgcc tgaactggaa
    12541 ggtgtcctcg aggcgcaggc ggaagctccg cacgaagtcc tcgctgaagc cgtgggaggc
    12601 gacgatgacg agctcgcccg actcgtcgaa ggcgaggacg gagccgaggt tcaccttctc
    12661 caggatccgg aacgcgtagt ccaggacggt ggagaagatc ttctggtcgt cctcgttgag
    12721 gaggatggcg cggttcacct cggaggcgag ctccccgagg agcctgttct tccgggcgcg
    12781 ccggtccgcg ccgtggtgcg cgcgcgcgac cagggcgcct tccagggtga gggtcaggca
    12841 gagggcggcc atggcctcgg acaggggcag ggtgtcgccc caggccgagg cgaggagggc
    12901 cagggcgagg agcggcatga cgaagaggat gacgagctgt atggtccgag agagatggag
    12961 ctgcctgatc gccgcctcct tcgccttcgt ctccgccgtc gcggcgcggg cctggatcgg
    13021 cccggcgcgc gaggcgtttc cttagtcccg ccgcctgatt tccgccacgg cctcgaggag
    13081 ggcccgcgcc ttgccgtagg ccgcctcgtc ggcgacgaag aggcggaggc cccagctccc
    13141 cttcgccttg cccgcggcca gggcttccgc gagcgggccg cccaaggcct tttccgcctc
    13201 ctcgcggagc tcctcgatct cggcttcccg ggcggcgacg ccgacgagga cgccggcggg
    13261 ggtcaccgtg atctcggcca gggccttgtc tccgtcatgg agcttgagct tccagccgta
    13321 tttcctgccg taatgcttcc aatcccgctc aaagccctcg gccgcggcca ggatgccttc
    13381 gtaccaggga tacatgccgc ccagggccgc ctggaccgcg gcgggttcgg ggcggggcgt
    13441 ctcttccatg aagtagtttt cgttcacgat ggcccattct agcccggaaa tcgcccggag
    13501 cggaaggccg ggccgcggga ggcgcgcgcg tccggtcatg gccgccgcgg gcaagtctcc
    13561 ggcccgcggc ccgcccaaga cgaggcgcgg gctttcccgg tatactcggc ccgggccccg
    13621 ggaggggccg cgatgttccc gcggttcgag gagccgacct tgcccttctc cgatctcccg
    13681 ctctcaagcg gcttaaggcg agcgataacg ggcctgggcg ccgtcgagcc ctttcccgtc
    13741 caggccgccg ccatccccct cgtcctcgcg ggccgtgacc tcgccgtcag cgcaaagacc
    13801 ggctcgggca agaccctggc cttcgccgcg ccgatcctgg accggctggc cctcgcgccc
    13861 gcgcgcaagg gtggattggt gcgcgccctc gtcctcgtgc cctcccgcga gctcgcggc
//
LOCUS       DAOOSJ010000035        13855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2953_length_13855_cov_6.856087, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000035 DAOOSJ010000000
VERSION     DAOOSJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2953_length_13855_cov_6.856087"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLG14_03105"
     CDS             <1..450
                     /locus_tag="PLG14_03105"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOX48149.1"
                     /translation="VVAAISCNKGSKTFQYAKHQIHLDVGPEVVTGSTRMKAGTAQKL
                     VLNMITTAAMIRIGKVYHNLMVDLSPVNRKLVERSKRLVRQATSCSKEEAERAFEASG
                     RRPKIAIIMVLLGVGREEAERLEKKGEGPISAAVRVYREERDGAGKD"
     gene            456..1322
                     /locus_tag="PLG14_03110"
     CDS             456..1322
                     /locus_tag="PLG14_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOX48150.1"
                     /translation="MDEERSIESALAAISARLDSLSESERKVAEFVVREPKKTLHFNV
                     SELARQSGSSQAAVVRFCKRIGLGSFNNFKLRLAKDVFRDADERFIPDLDLQSEASPE
                     RVIQDVILRSQRGLTLLGSVLDPKAVEKAADAILGATTTALFGVGASGVVAYDFLQKL
                     IRVGLPSTYTLDTDLQVTAASGLRPTDAAFVVSYSGENAAMCEAARQAKGRGATLISL
                     TMDSPNAVRALSDISLLVPASERIYRQGAVTSRINQLTVIDILYSIIVSRNLDSSIAA
                     IERTMQATHKRK"
     gene            1527..3230
                     /locus_tag="PLG14_03115"
     CDS             1527..3230
                     /locus_tag="PLG14_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48151.1"
                     /translation="MKKSHIGLRLALFAALASTLVLGALAGVQYALAKAEVTSRFAAR
                     AELMADRLSQNLEESLLNADAEQAAKIVSTELREGISPAVLVFAGDAFFAGAEADETG
                     SVSVLAAAKELPGLGSVERTVLRSGRVLGAVRVYLDPAPVRAELRSRLATSALSSLAV
                     ALALVAILLSVSRAFVTRPLGHVNDFLGRLASGSGDLTVAVPVARDDEIGAIAAGVNA
                     FRLSLAGLLRSIKASAEALSADSLELSANTEETASAVVEIEANMRGVQRQIEALSEIV
                     EEVKARAAAIGATTGAQRAAAERQEGLVRGAAGTVERLAERSTVVAQGASASLASYER
                     LGSASERGKEALGLVSESIAKVSGMSDGLQEAATIIQNIASQTNILAMNAAIEAAHAG
                     EAGKGFAVVSDEIRRLSEDSAEQAKLTKTSLDAIDLALKELLGPFGVLSGGFAEITDL
                     VAEVSAYARASEEAARVEERLGAEIQEALGGVTALSAELSGATRGAEEEAAGILGAVE
                     RLYELARVSEGGMTEMTLGVGQIAEAVRSINELSGRNKDSIEALLGQAASIKTEADGK
                     A"
     gene            3217..3543
                     /locus_tag="PLG14_03120"
     CDS             3217..3543
                     /locus_tag="PLG14_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48152.1"
                     /translation="MGRPSRQDGPEPEKELSGEALAKEVARLIRLARRHWEAHENGAC
                     RAERDRALALYPGLRPEDKKRIPPALLVWLRYRSEKYFGEGRTPPGGPRKGGARKGGK
                     APKPRP"
     gene            complement(3586..4767)
                     /locus_tag="PLG14_03125"
     CDS             complement(3586..4767)
                     /locus_tag="PLG14_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48153.1"
                     /translation="MKGRLGGLVRDPASQLGFLHVLNDGWFASLPLLLPFIQGELGID
                     FAQVGLLSSLLSLAGVALALPAASIARKAGGLRTLVAGALLYSLAFVAVGLSAGFPSL
                     VLAFGLASVGFGLFHPVGFAMVAHGSGPEELGRRMGGFTAVGDIGRVGVAACVTLLVA
                     LAGWRRTALAYGCVPAVLALGLYAASRGSPARSGPEESGGKARGLRRNRRFLLATASG
                     FIDALASSSLFVFIPFLYIHRGASTALLGSLSGAFFVGNMLGKVVLGRIADRLGSRKV
                     FVLSELLMAGLLVALGAAKSLPALVVVSVLLGAVTKGTVPVLNTIIAASVPDRRLFDK
                     AFGAGAFANGVAAAAAPLLFGWVIDERGIVAVFGVCAAFAVAAVVPVAVGALLERRAA
                     A"
     gene            5039..7930
                     /locus_tag="PLG14_03130"
     CDS             5039..7930
                     /locus_tag="PLG14_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48154.1"
                     /translation="MSPVLDFFKRLFSKETPPEPGSIAKELWIADFSSPEKGRFPAEK
                     AESHAASYEDGALVLDLRRERLYAWTEATLYRYDDFVLEGEFEFAPEAASAAVGALDA
                     TGAAGGPASTGPGSAGAAGASGGGAGETEAGSAAAGFLFRYADEGNFYSALVSDRGYF
                     RLDAVFNGKPRPLIAWTRAKEGEKDGRIADFSLRIIARGERLCLEVDGLWVGEAVDDE
                     LRSGFVAFAGQNYGASASARLALRSAFVESRPVEVETWYYRHSYFEAVDPGARFRLAE
                     TFFSMGEWLKAAVQLRKIERKRPLSPPELFLKAEAALRLELLDEAEQSIEACLASAPA
                     MREAVEEKANLLYLRGRYPELRDYAAGILADAPDNGRLRGLLGHARFNLGDYRGAAED
                     YGRAAALEPGQPLFRMNEARALDQAGDKAAAAEAYAAAARGFYEAEADDDLALALGRL
                     SALRPRAAETRELKAKALFRAGRKEEARKLIEALLDKGSSDSALHYLAGILASERGDR
                     EKALERYSRALELEPAYPLYAFRRAEALFLLGRPEAEEAIARALELAPEEGWTRNLAA
                     LAILKSADEGAESLSPELAAKARALLAGARAALPEAPEPAINLAWLESLEGRLDEALS
                     VLAHFPASAAARNEAGNALARAARAAGAAATAEAAGRSGAQAAAPAAATAAGPDGGAA
                     EAAGRSGAQGAAEASSAREDLLERAAKEYEAATALDPGKAEYQTNLAAAYLELERFGD
                     AEERLRRALDLGPEPRAYLLAGNLGAVYGDRIRAEAAYRLGLELAPGDSALLFALGRS
                     YLSGGKSAKAEECRARLADVDAGRAARLGEEILEATTVRTACASCGRVWRSPRVLPAQ
                     SAASIRAMPPDDSPAGACPTCGRVFCIACRKGHLAENRFTCPSCGEALKLSDDRLRYL
                     VREHLRRARAGGDAGGVEAAAASGGAEGTGAGDAANGGEGAGGGEGQKA"
     gene            8010..8738
                     /locus_tag="PLG14_03135"
     CDS             8010..8738
                     /locus_tag="PLG14_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOX48155.1"
                     /translation="MNLEQNPRGNLVKHGALLLALALGLAVLPAAAPAWAQDILTADQ
                     FFSQVADRYAQVADYEGRINIAAGKDVMSGVIAYKAPSLLRIDFVQPPDQVIAFDGQR
                     LVVYIPSLRAVLQQQAAGKPGAGSAGLATREGLKMMKRNYTVAYEASPAAEPLDAATP
                     EPVVRLALNRKSVAEGFRTMRLSVSPETKLIRRIEGTTVSGERFVFDFVGIKLNQGIP
                     EARFIYDSPASANVYNNFLFGSDN"
     gene            8758..9894
                     /locus_tag="PLG14_03140"
     CDS             8758..9894
                     /locus_tag="PLG14_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX48156.1"
                     /translation="MSPIGETLRAAREQKGLSLDRVADETNIAKRYLAALEAEDFGVF
                     PGDPYAIGFLRNYAGYLDLAPDELVATFKNMRIQEQPVPIQELIPRRGLPPLAVAGIA
                     LGGVLVLAVAAVLAFGGRSPSAVSEIAPEDRAPVEYRVEGARFERRLYEGDALLVAVG
                     QDSYKILLAKIDDAVSLESPVGKSRLMLGEEGTLDLDRNGTPELKLLVSDFQKRSPLK
                     GAVLRVEYADPAAATAAAAAPAADPAAAPPPAVPATAAAPAPAAAVSAAAKSVVLFES
                     AKTAYPFVLNVTFRGSCMFRYEIDRRDRDERYYHKGETITVNANNSIKIWASNAQSAK
                     VTIQASGGKSVDLDLGGAGEIAVKRIAWSQTESGGYALSALEVD"
     gene            9945..11276
                     /gene="rimO"
                     /locus_tag="PLG14_03145"
     CDS             9945..11276
                     /gene="rimO"
                     /locus_tag="PLG14_03145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOX48157.1"
                     /translation="MRFYLDQRGCAKNQVDGEEIGARLEASGHAWAPSADEADLIIVN
                     SCGFIEDAKKESLDAVLDWKARYPGKRVLLAGCLAQRYAADLAEGLPEADGIVGNADL
                     AAVVAAAEETAAGGRPVRVPEAAPSIGAFRRGRLLDFPGTAHVKITEGCSNGCSYCAI
                     PLIRGSLKSRPVEEVVAECRDLVARGIRELVLVGQDLGAYGRELSGGELLPPLLSALS
                     ATPGDFRVRTLYIHPDNFPYAILPIIKADPRLLPYFDLPFQHASAPVLRAMNRKGSPE
                     AYLRLLSRIREALPDAVIRSTFLLGFPGETDEDFAALRGFQEAARIDWLGAFAYSREE
                     GTAAYAMKGRVPKKTVAERKKAVEEAQERITSERLARFVGRELEVLVEEPVEGAAEDR
                     DGGEELSLGRAWLQAPEVDGLTVLRGRFEAGTAVRAKVLAVNGVDLDARPL"
     gene            11287..13848
                     /locus_tag="PLG14_03150"
     CDS             11287..13848
                     /locus_tag="PLG14_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX48158.1"
                     /translation="MPGDESLPPYLASLNPEQLAAVRHEGRPLLILAGAGSGKTRVIT
                     TKIAWLVRERGYDPESILAVTFTNKAAREMRDRAASLEPACARAVIRTFHSFGAWFLR
                     RNAAAAGLDSNFTIYDDDDQATLLHGALPQYARNECSRIVQSIARAKDFGLEPDSPDL
                     SRAFSDPAMRRVYAIYEERLRATGNVDFGDLIRLPAKLLSGDEAIRRRTRQRFRVILV
                     DEYQDSNVAQFELLRLLAGAGAPGEGGDRLPVPPAYVCVVGDDDQSIYRFRGAEVKNI
                     LSFPELFPGTEIVRLERNYRSYQSILDVAGDVVSHNEGRLGKELRAERRGGRPPRLSL
                     LEDQDEETRFCARICEERVKAGGKWGDVAILYRTNAQSLSFEKEFPRRGIPYRIVGAL
                     RFYDREEVKDLLAYLALLLNGKDEVAFKRVVNKPSRGIGETSVDALCAAALDPELGHG
                     GDLLSASAAEADNLRGRAKSGLREFLSVVKGLRAALGLIAAPGAEAAASTRAGDEPAP
                     PDASTSASPDGATRAPASAQPEDTLRDSPPLDARDRKKAGMKPQGEGSLGDVVERLAR
                     DSGLLEYHRGQDEVAGTQKAANVDELVNAATLYPRSAEGLAEFLETIELDRSLTAEES
                     TADAVTLITMHNTKGLEFPVVVATGIEQGLFPRDDDEGEELEEQRRLFYVAITRAKDE
                     LHLTACRWRRIHGRLFETLPSRFLKEIAPDKLEFAGAGAGGQGRAGGSGLAAQGGQLS
                     SAGRLGGRQGAYSGGGAGGYPSGGAGGGSGGYAGGYTGGAGAARRPGGAAGKAAEASP
                     WRAGQAVYHEDYGSGVIIKVQPTPNSGPLVVVRFETGKQAQFFPKFSTKLEPLRG"
BASE COUNT         2102 a         5279 c         4744 g         1730 t
ORIGIN      
        1 gtcgtcgcgg ccatcagctg caacaagggc tccaagacct tccagtacgc gaagcaccag
       61 atccacctgg acgtcgggcc cgaggtggtc accggctcca cgaggatgaa ggcgggcacg
      121 gcccagaagc tcgtcctcaa catgatcacg acggccgcga tgatccgcat cggcaaggtc
      181 taccacaacc tcatggtcga cctctcgccc gtgaaccgca agctcgtgga gcgctccaag
      241 cgcctggtcc gccaggccac ctcctgctcc aaggaggagg cggagagggc cttcgaggcc
      301 tctggacgaa ggcccaagat cgccatcatc atggtcctcc tcggcgtggg ccgggaggag
      361 gccgagcgcc tcgagaaaaa gggcgaaggc ccgatctccg cggccgtccg cgtctacagg
      421 gaagagcggg acggggccgg caaggactga tggcgatgga cgaggagagg agcatcgaga
      481 gcgcgctcgc ggcgatctcg gccaggctgg acagcctgtc cgagtccgag cgcaaggtgg
      541 cggagttcgt cgtgcgcgag cccaagaaga cgctccactt caacgtgagc gagctcgcga
      601 ggcagtcggg ctcgagccag gcggccgtcg tgcgcttctg caagcgcatc ggcctcggct
      661 ccttcaacaa cttcaagctc cgcctcgcga aggacgtctt cagggacgcc gacgagcgct
      721 tcatccccga cctggacctc cagtccgagg cctcgcccga gcgggtcatc caggacgtga
      781 tcctccgctc ccagcgggga ctcaccctcc tcggctccgt cctcgatccc aaggcggtgg
      841 agaaggccgc cgacgccatc ctcggcgcga cgacgacggc cctcttcggc gtcggcgcct
      901 ccggcgtcgt cgcctacgac ttcctccaga agctcatccg ggtgggcctc ccctcgacct
      961 acaccctgga caccgacctc caggtgacgg cggcctcggg cctcaggccc acggacgcgg
     1021 ccttcgtcgt ctcctactcg ggggagaacg ccgcgatgtg cgaagccgcg cgccaggcca
     1081 agggcagggg cgcgaccctg atctccctca cgatggacag ccccaacgcc gtccgcgccc
     1141 tctccgacat cagcctcctc gtccccgcct ccgagcgcat ctaccgccag ggcgccgtca
     1201 cctcccgcat caaccagctc accgtcatcg acatcctcta ctccatcatc gtgtccagga
     1261 acctggactc ctccatcgcc gcgatagagc ggacgatgca ggccacccac aagcggaaat
     1321 agccgccgcc cgctcccggc gcggcccgga aggcgggaag cctgctttgc ggcgcgcggc
     1381 gcggcccggc cgggaggaac acccttgccg cgcgcgggtg gcgcggcccg gccgaacggc
     1441 ccaggcgcgc ctccgctcga tcccgctttc ctcccgcgga atacctgcta tactccctcc
     1501 gtcaacgcct tttccaggag cgcttcatga agaagagcca tatcggcctc aggctggccc
     1561 ttttcgcggc cctggcctcg accctcgtcc tcggagccct ggcgggcgtc cagtacgccc
     1621 tggccaaggc ggaggtgacg agccgcttcg ccgccagggc cgagctcatg gccgacaggc
     1681 tctcccagaa cctggaggag tccctcctga acgccgacgc cgagcaggcc gcgaagatag
     1741 tctccaccga gctccgcgag ggcatctcgc cggccgtcct cgtcttcgcg ggcgacgcct
     1801 ttttcgccgg cgccgaggcg gacgagacgg gctcggtctc cgtcctcgcg gccgccaagg
     1861 agcttcccgg gctcgggagc gtcgagcgca cggtgctccg ctcgggcagg gtgctcggcg
     1921 ccgtgcgggt ctacctggat ccggccccgg tccgcgccga gctccggtcc aggctcgcga
     1981 cctcggccct ctcgagcctg gccgtggccc tggccctggt ggccatcctc ctgtccgtct
     2041 ctcgcgcctt cgtgaccagg cccctcggcc acgtcaacga cttcctgggc cgcctggcct
     2101 cgggatcggg ggacctcacc gtcgccgtcc cggtcgcgcg ggatgacgag ataggcgcca
     2161 tcgcggcggg cgtgaacgcc ttccgcctga gcctggccgg cctcctccgc tcgatcaagg
     2221 ccagcgccga ggccctctcc gcggattcgc tggagctctc cgcgaacacc gaggagaccg
     2281 cgagcgcggt cgtcgagatc gaggccaaca tgcgcggcgt gcaaaggcag atcgaggcgc
     2341 tctccgagat cgtcgaggag gtcaaggcga gggccgcggc gatcggcgcg acgaccggcg
     2401 cccagcgggc ggccgcggag cgccaggagg gcctggtgcg cggcgccgcc gggacggtcg
     2461 agcgcctggc cgagcgctcc acggtcgtgg cacaaggggc cagcgcctcc ctcgcctcct
     2521 acgagcggct cggctccgcc tccgagcgcg gcaaggaggc cctgggcctc gtctccgagt
     2581 ccatcgcgaa ggtctccggc atgtccgacg gcctccagga ggcggcgacg atcatccaga
     2641 acatcgcgag ccagacgaac atcctggcca tgaacgccgc catagaggcg gcccacgcgg
     2701 gcgaggcggg aaagggcttc gccgtggtct ccgacgagat ccgccgcctc tccgaggact
     2761 ccgccgagca ggccaagctg acgaagactt ctctggacgc gatcgacctc gcgctcaagg
     2821 agctgctcgg gcccttcggc gtgctctccg gcggcttcgc cgagatcacc gacctggtcg
     2881 ccgaggtgag cgcctacgcc cgggcgagcg aagaggccgc ccgggtcgag gagaggctgg
     2941 gcgccgagat ccaggaggcc ctgggcggcg tcacggccct gagcgcggag ctctccggcg
     3001 cgacccgcgg cgcggaggag gaggcggccg gcatcctggg cgccgtcgag cgcctgtatg
     3061 agctggcccg cgtctccgag ggcggcatga cggagatgac cctcggcgtg ggccagatcg
     3121 ccgaggcggt ccgctccatc aacgagcttt ccgggcgcaa caaggattcc atcgaggctc
     3181 tcctgggcca ggcggcctcc atcaagacgg aagccgatgg gaaggcctag ccggcaggac
     3241 ggcccggagc cggagaagga gctctccggc gaggccctcg ccaaggaggt cgcgcggctc
     3301 atcaggctcg cccgcaggca ctgggaggcc cacgagaacg gggcctgccg cgccgagcgg
     3361 gaccgcgccc tcgccctcta ccccgggctc aggcccgagg acaagaagcg gattcccccg
     3421 gccctcctcg tctggctcag gtacaggagc gagaaatact tcggcgaggg gaggacgccc
     3481 cccggcgggc cgcgcaaggg cggagcgcga aaaggcggga aagcccccaa gccccggcct
     3541 tgagcggccc gggagggcgg aggcagggct tgaggaagcc cggccctagg ccgccgcccg
     3601 ccgctccagc aaggccccga ccgccacggg aacgacggcc gcgacggcga aggccgcgca
     3661 gaccccgaag acggccacga tgccccgctc gtcgatcacc cagccgaaga gcagcggcgc
     3721 ggccgcggcg gccacgccgt tggcgaaggc gccggcgccg aaggccttgt cgaagagccg
     3781 cctgtcgggc acggaagccg cgatgatcgt gttgaggacg gggaccgtcc ccttcgtgac
     3841 cgcgcccagg aggaccgaga cgacgaccag cgcggggagg ctcttcgccg cgccgagcgc
     3901 gacgaggagt cccgccatga ggagctcgga gaggacgaag accttgcggc tcccgaggcg
     3961 gtcggcgatc cttcccaaga cgaccttgcc gagcatgttg ccgacgaaaa aggccccgga
     4021 gagggatccg agcagggccg tggaagcccc gcgatggata tagaggaagg ggatgaagac
     4081 gaagagcgag gagctcgcga gggcatcgat gaagccgctc gccgtggcga ggaggaagcg
     4141 gcggttccgc ctgagaccgc gagccttgcc gcccgactcc tcgggcccgc tcctcgcggg
     4201 gctccccctc gacgccgcgt acaggcccag ggccaggacg gcggggacgc agccgtaggc
     4261 cagggccgtc cgcctccagc ctgcgagcgc gacgaggagg gtgacgcagg ccgcgacgcc
     4321 gacccgcccg atgtccccga cggcggtgaa gccgcccatc ctcctcccga gctcctccgg
     4381 tccgcttccg tgggccacca tggcgaatcc gacggggtgg aaaagcccga agccgaccga
     4441 ggccaggccg aaggccagga cgagggaggg gaagccagcg gaaagcccca ccgcgacgaa
     4501 ggcgagggaa tagagcaggg cgccggccac gagggtccta aggcccccgg ccttcctggc
     4561 gatggaggcc gcgggcaggg ccagggccac gcccgccagg ctgaggaggc tcgagaggag
     4621 gccgacctgg gcgaaatcga tgccgagctc gccctggatg aaggggagga ggaggggcag
     4681 gctcgcgaac caaccgtcgt tgagcacgtg gaggaagccc agctggcttg ccggatcgcg
     4741 gacgagcccg ccgagtcttc ccttcatgcc gcgcatcgcc tctcctctcc gccggatgcg
     4801 gcgcggggct tcggcccgtc ggcgctccgc cctgccggcc ccgccccgcc ttgccccgcg
     4861 ccgcgatgat acggaatccc ggggccgccg cgccaggggg ggggctcagg gacgccgggc
     4921 ggccgcgcca aggacaaggc ccggcgccgg ggcagcgaag ggaggtcgcg tcgggccctc
     4981 gcccttgccc cgccccgcga aaacccgtag tattggctgt cgaaaggaag gccgcccttt
     5041 gagccccgtt ctggacttct tcaagaggct tttctccaag gagacgccgc ccgagccggg
     5101 ctccatcgcc aaggaactct ggatagccga cttttcctcg cccgaaaagg ggagattccc
     5161 cgcggagaag gccgaatccc acgcagcctc ctacgaggac ggggccctcg tcctggacct
     5221 gcgccgcgag cggctctacg cctggaccga ggcgaccctc taccgctacg acgacttcgt
     5281 cctcgagggc gagttcgagt tcgctccgga ggcggcttcc gcggccgtcg gcgccctaga
     5341 cgcgaccggc gcggcgggcg gcccggccag cacgggcccg ggcagcgcag gcgcggctgg
     5401 cgcgagcggg ggcggggccg gcgagaccga ggccggaagc gccgccgcgg gctttctctt
     5461 ccgctacgcc gacgagggca acttctactc cgccctcgtc tccgaccgcg gctacttccg
     5521 cctggacgcc gtcttcaacg ggaagccccg ccccctcatc gcctggactc gggcgaaaga
     5581 gggcgagaag gacggccgga tcgccgactt ctccctgcgg atcatcgcca ggggcgagcg
     5641 cctctgcctg gaggtcgacg ggctctgggt cggcgaagcg gtcgacgacg agctcaggtc
     5701 gggcttcgtc gccttcgcgg ggcagaacta cggcgcttcg gcctccgcgc gcctggccct
     5761 gcgctcggcc ttcgtcgagt cgaggcccgt cgaggtcgag acctggtact accgccactc
     5821 ctacttcgag gccgtggacc cgggagcgcg tttccgcctg gcggagacct tcttctccat
     5881 gggcgagtgg ctcaaggccg ccgtccaact ccgcaagatc gagcgcaaac gccccctctc
     5941 cccgcccgag ctcttcctca aggccgaggc cgccctgcgc ctcgagctcc tggacgaggc
     6001 ggagcagtcc atagaggcct gcctcgcctc ggcccccgcg atgcgcgaag ccgtggagga
     6061 gaaggccaac ctcctctacc tccgcggccg ctaccccgag ctcagggact acgccgcggg
     6121 gatcctggcc gacgcgccgg acaacggccg cctccggggc ctcttgggcc acgcccgctt
     6181 caacctcggg gactaccgcg gggcggcgga ggactacggc cgggcggcgg ccctggagcc
     6241 gggacagccc ctcttcagga tgaacgaggc gcgggccctg gaccaggccg gggacaaggc
     6301 ggccgccgcc gaggcctacg ccgccgccgc ccggggcttc tacgaggccg aggccgacga
     6361 cgacctggcc ctggccctgg gccgcctctc cgcccttcgc ccccgggcgg ccgagacgag
     6421 ggagctcaag gccaaggccc tcttccgcgc cggcaggaag gaggaggcga ggaagctcat
     6481 cgaggctctc ctggataagg gctcctccga ctcggccctc cactacctgg ccggcatcct
     6541 cgcctccgag cggggcgatc gggagaaggc cctggagcgc tattcgcggg ccctggagct
     6601 cgagcccgcc taccccctct acgccttccg ccgcgcggag gccctcttcc tcctcggaag
     6661 gcccgaggcg gaggaagcca tagcccgcgc cctcgagctc gcgccggagg agggctggac
     6721 ccggaacctc gcggccctcg ccatcctcaa gtccgcggac gagggagccg agtccctctc
     6781 gcccgagctc gccgcgaagg cccgcgccct cctcgccggg gcccgggccg ccctccccga
     6841 ggcgcccgag cccgcgatca atctcgcctg gctcgagtcc ctggagggga ggctggacga
     6901 ggccctctcc gtcctggcgc atttccccgc ctccgccgcc gcgcggaacg aggcgggcaa
     6961 cgccctggcc cgggccgcga gggcggcggg agccgccgcg accgcggagg ccgccggccg
     7021 gtccggcgcg caggccgccg cccccgccgc cgcaaccgcc gcaggcccgg acggaggcgc
     7081 cgcggaggcc gccggccggt ccggcgcgca gggcgccgcg gaggcaagct ccgcccgcga
     7141 ggacctcctc gagcgcgccg cgaaggaata cgaggcggcg acagccctcg atcctgggaa
     7201 ggccgagtac cagaccaacc tggccgcggc ctacctggag ctcgagcgct tcggcgacgc
     7261 cgaggagcgg ctccggaggg ccctggacct gggccccgag ccgagggcct acctcctcgc
     7321 gggcaacctg ggcgcggtct atggagaccg gatcagggca gaggccgcct atcgcctggg
     7381 cctcgagctc gcgcccggcg actccgccct cctcttcgcc ctgggccgct cctacctctc
     7441 cggcggcaag agcgcgaagg ccgaggagtg cagggccagg ctggccgatg tcgacgcggg
     7501 ccgggccgcc cgcctcggag aggagatcct ggaggcgacg acggtccgca cggcctgcgc
     7561 ctcctgcggg agggtctggc ggagcccccg cgtactcccc gcccagagcg ccgcgagcat
     7621 cagggccatg ccgcccgacg actccccggc aggcgcctgc ccgacctgcg gccgcgtctt
     7681 ctgcatcgcc tgccgcaagg gccacctcgc ggagaaccgc ttcacctgcc cgagctgcgg
     7741 ggaggcgctg aagctctccg acgaccgcct gcgctacctc gtccgcgagc accttcgccg
     7801 cgcgagggcc ggcggggacg cgggcggcgt ggaagccgcg gcagcctcag gcggcgcgga
     7861 agggacgggg gccggggatg ccgccaacgg cggcgaaggc gcggggggcg gcgaagggca
     7921 gaaggcctag gatggcgcgg cccgcggcgc ggcggaacgg ccgcgcgggc gaccttgtct
     7981 ttcccgccgc cgctctggta ctataggccg tgaatctcga acagaatccc aggggaaacc
     8041 tcgtgaagca tggagccctc ctcctcgccc tggccctcgg cctagccgtc cttccggcgg
     8101 ccgcgccggc ctgggcccag gacatactca ccgccgacca gttcttctcg caggtcgccg
     8161 accgctacgc ccaggtcgcc gactacgagg ggaggatcaa catcgccgcc ggcaaggacg
     8221 tcatgtcggg cgtcatcgcc tacaaggcgc cctccctcct ccgcatcgac ttcgtccagc
     8281 cgcccgacca ggtgatagcc ttcgacggcc agcgcctcgt cgtctacatc ccgagcctcc
     8341 gggccgtcct ccagcagcag gccgcgggca agcccggcgc cggctcggcc ggcctcgcga
     8401 cccgcgaggg gctcaagatg atgaagcgga actacacggt ggcctacgag gccagccccg
     8461 cggccgagcc cctcgacgcg gccacgcccg agcccgtcgt gcggctcgcc ctcaaccgca
     8521 agtccgtcgc ggagggcttc aggacgatgc gcctctccgt gagccccgag accaagctca
     8581 tcaggcggat cgagggcacg accgtctccg gcgagcgctt cgtcttcgac ttcgtcggaa
     8641 tcaagctgaa ccagggcatt cccgaggcgc gcttcatcta cgactcgccg gcttcggcca
     8701 acgtctacaa caacttccta ttcggatcag acaactaaga aaaggacggg accgcacatg
     8761 tcgcctatcg gagagacgct ccgcgccgcg cgcgaacaga agggactcag cctcgatcgg
     8821 gtggccgacg agaccaacat cgcgaagcgc tatctcgccg ccctcgaggc cgaggacttc
     8881 ggcgtcttcc ccggcgaccc ctacgccatc ggcttcctgc gcaactacgc gggctacctg
     8941 gacctggccc cggacgagct cgtcgcgacc ttcaagaaca tgaggatcca ggaacagccc
     9001 gtgccgatcc aggaactgat ccccaggcgc ggccttccgc ccctcgcggt ggcgggcatc
     9061 gcccttggcg gcgtcctcgt cctcgccgtc gcggccgtcc tcgccttcgg cggccgctcc
     9121 ccctccgcgg tctccgagat cgccccggag gacagggccc ccgtggagta ccgcgtcgag
     9181 ggcgcgcgct tcgagcgccg cctctacgag ggcgacgccc tcctcgtcgc cgtcggccag
     9241 gacagctaca agatcctcct cgcgaagatc gacgacgcgg tgagcctcga gagccccgtc
     9301 ggcaagagcc gcctcatgct cggggaggag ggcaccctcg acctggaccg gaacgggacg
     9361 cccgagctca agctcctcgt gagcgacttc cagaagcgct cccccctcaa gggcgccgtc
     9421 ctccgcgtcg agtacgccga ccccgcggcg gcgaccgccg ccgcggccgc gcccgccgcc
     9481 gacccggccg cggcgccccc cccggccgtc cccgcgacgg ccgccgcgcc cgctccggcg
     9541 gccgccgtct ccgccgcggc caagagcgtc gtcctcttcg agtccgccaa gaccgcctat
     9601 cccttcgtcc tcaacgtcac cttccgcggc tcctgcatgt tccgctacga gatcgaccgc
     9661 cgcgaccggg acgagcgcta ctaccacaag ggcgagacga tcaccgtcaa cgccaacaac
     9721 tcgatcaaga tctgggcctc caacgcccag tcagcgaagg tcacgatcca ggcctccggc
     9781 ggcaagtccg tcgacctgga cctcgggggc gccggggaga tcgcggtcaa gaggatcgcg
     9841 tggtcccaga ccgagtcggg cggctacgcc ctctccgccc tcgaagtgga ctgaccccgg
     9901 ccgcaagggg cgcccctggc cgggcgcccc gacacaagga ccagatgcgt ttctatctcg
     9961 accagcgcgg ctgcgcgaag aaccaggtcg acggcgagga gatcggcgcc aggctcgagg
    10021 cctcgggcca cgcctgggct ccctccgccg acgaggccga cctcatcatc gtcaactcct
    10081 gcggcttcat cgaggacgcg aagaaggagt ccctggacgc cgtcctggac tggaaggccc
    10141 gctaccccgg aaagcgcgtc ctcctcgcgg gctgcctggc ccagcgctac gccgccgacc
    10201 tcgcggaggg cctgcccgag gccgacggca tcgtcggcaa cgccgaccta gccgccgtgg
    10261 tcgccgcagc ggaggagaca gcggcgggcg gcaggcccgt gcgcgtgccc gaggccgcgc
    10321 cctccatcgg cgccttccgc cggggccgcc tcctcgactt ccccggcacc gcccacgtga
    10381 agatcaccga gggctgctcc aacggctgct cctactgcgc catccccctc atccgcggct
    10441 ccctcaagtc ccgccccgtg gaggaggtcg tcgccgagtg ccgcgacctc gtggcgcgag
    10501 ggatcaggga gctcgtcctc gtgggccagg acctgggcgc ctacggccgc gaactctcag
    10561 gcggcgagct ccttccgccc ctcctttcgg ccctctccgc gacgcccggc gacttccgcg
    10621 tccggaccct ctacatccac cccgacaact tcccctacgc gatcctcccg atcatcaagg
    10681 ccgacccgcg cctccttccc tacttcgacc tacccttcca gcacgcatcc gccccagtcc
    10741 tccgcgcgat gaaccggaag gggagccccg aggcctacct ccgcctcctc tcccggatca
    10801 gggaggccct gcccgacgcg gtgatccgct cgaccttcct cctcggcttc ccgggggaga
    10861 ctgacgagga cttcgcggcc ttgcgcggct tccaggaggc cgcgaggatc gactggctcg
    10921 gcgccttcgc ctactcccgc gaggagggca cggcggccta cgcgatgaag ggcagggtcc
    10981 cgaaaaagac cgtcgccgag cggaagaagg ccgtggagga ggcgcaggag cgcataactt
    11041 ccgaacgcct cgcgcgcttc gtcggccgcg agctcgaggt cctcgtggag gagccagtgg
    11101 agggcgccgc cgaggaccgg gacgggggcg aggagctctc cctgggcagg gcctggctcc
    11161 aggcccccga ggtcgacggc ctcacggtcc tgcgcggccg cttcgaggcg gggacggccg
    11221 tcagggcgaa ggtcctcgcg gtgaacggcg tcgacctgga cgcccggccc ctttaggcgg
    11281 ctggccatgc ccggcgacga gtcccttccg ccctacctcg cctccctcaa ccccgagcag
    11341 ctcgccgccg tgcgccacga agggaggccc ctcctcatcc tcgcgggcgc gggctccggc
    11401 aagacccgcg tcatcacgac gaagatcgcc tggctggtgc gcgagcgggg ctacgacccc
    11461 gagtccatcc tggccgtcac cttcacgaac aaggccgcgc gcgagatgcg cgacagggcg
    11521 gcctccctgg agcccgcctg cgcccgcgcc gtcatcagga ccttccactc cttcggcgcc
    11581 tggttcctca ggcggaacgc ggccgccgcc ggcctggact ccaacttcac gatctacgac
    11641 gacgacgacc aggcgaccct cctccacggc gccctgccgc agtacgcgcg gaacgagtgc
    11701 tcgcgcatcg tccagtcgat agcccgcgcg aaggacttcg gcctcgagcc cgactccccc
    11761 gacctctcgc gggccttctc cgacccggcc atgaggcggg tctacgcgat ctacgaggag
    11821 cggctcaggg cgacgggcaa cgtcgacttc ggcgacctca tccgcctccc cgcgaagctc
    11881 ctctcgggcg acgaggcgat caggcgccgc acgcgccagc gcttccgcgt catcctcgtc
    11941 gacgagtacc aggactccaa cgtcgcccag ttcgagctcc tgcgcctcct cgcgggcgcg
    12001 ggcgcgcccg gggagggagg ggaccgcctg cccgtgccgc cggcctacgt ctgcgtcgtc
    12061 ggcgacgacg accagtcgat ctaccgcttc cgcggggccg aggtgaagaa catcctctcc
    12121 ttccccgagc tcttccccgg gacggagatc gtgcgcctgg agcggaacta ccgctcctac
    12181 cagagcatcc tggacgtcgc gggcgacgtc gtgtcgcaca acgagggcag gctcggcaag
    12241 gagctccggg cggagcgccg gggcggaagg ccgccccgcc tcagcctcct cgaggaccag
    12301 gacgaggaga cccgcttctg cgcccggatc tgcgaggagc gcgtgaaggc gggcggcaag
    12361 tggggcgacg tcgccatcct ctaccgcacg aacgcccaat ccctctcctt cgagaaggag
    12421 ttcccccgcc gcggcatccc ctaccgcatc gtcggagccc tgcgcttcta cgaccgcgag
    12481 gaggtgaagg acctcctggc ctacctggcc ctgctgctca acggcaagga cgaggtcgcc
    12541 ttcaagcgcg tggtcaacaa gccgagccgc ggcatcggcg agacgagcgt cgatgccctc
    12601 tgcgccgccg ccctcgaccc tgagctgggc cacggcggcg acctcctctc ggcctccgcc
    12661 gccgaggcgg acaacctccg cggccgcgcc aagtcgggcc tcagggagtt cctctccgtc
    12721 gtgaagggcc tccgcgcggc cctgggcctg atagccgcgc cgggcgccga ggcggccgcg
    12781 tctacgaggg ccggcgacga gccggcgccg ccagacgcct cgacgagcgc ctcacctgac
    12841 ggcgccacgc gggcaccggc ctccgctcag cccgaggaca cgctccggga cagccccccc
    12901 ctcgacgccc gcgaccgcaa gaaggccggc atgaagcctc aaggcgaggg gagcctgggc
    12961 gacgtcgtcg agcgcctggc ccgcgactcg ggcctcctcg agtaccaccg cggccaggac
    13021 gaggtcgccg gcacgcagaa ggcggccaac gtcgacgagc tcgtcaacgc ggcgaccctc
    13081 tatccccgga gcgccgaggg cctggccgag ttcctggaga ccatcgagct cgaccgcagc
    13141 ctgaccgcgg aggagtcgac ggccgacgcc gtgaccctca tcacgatgca caacacgaag
    13201 ggcctcgagt tccccgtcgt cgtcgcgacc ggaatcgagc agggcctctt cccccgcgac
    13261 gacgacgagg gcgaggagct cgaggagcag cgccgcctct tctacgtcgc catcacgcgg
    13321 gccaaggacg agctccacct caccgcctgc cgctggcggc gcatccacgg gcggctcttc
    13381 gagaccctcc cctcccgctt cctcaaggag atagccccgg acaagctcga gttcgcgggc
    13441 gcgggggccg gcggccaggg ccgcgcgggg gggagcggcc tcgccgccca gggaggacag
    13501 ctttcctcgg cgggccggct cggcggccgc cagggggcgt actccggcgg cggcgcgggc
    13561 ggctatccga gcggcggcgc cggcggcggt tctggcggtt acgccggcgg ctacaccggc
    13621 ggcgcgggcg cggcccggcg gcccggcggc gcggcgggca aggcggcgga ggccagcccc
    13681 tggcgggccg gccaggccgt ctaccacgag gactacggct cgggcgtcat catcaaggtc
    13741 cagccgacgc cgaactcggg ccccctcgtc gtcgtccgct tcgagacggg caagcaggcc
    13801 cagttcttcc cgaagttctc cacgaagctc gagccgctcc gcggctaggc cccag
//
LOCUS       DAOOSJ010000036        13814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2962_length_13814_cov_6.011992, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000036 DAOOSJ010000000
VERSION     DAOOSJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_13814_cov_6.011992"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLG14_03155"
     CDS             complement(<1..239)
                     /locus_tag="PLG14_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX48159.1"
                     /translation="MHYSLGDYVLDIAQNAVESGAGTVRIDFAETEESFSVAVADDGV
                     GMAEEELARALDPFYTDGKKHARRKVGLGLPFLVQA"
     gene            complement(262..738)
                     /locus_tag="PLG14_03160"
     CDS             complement(262..738)
                     /locus_tag="PLG14_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOX48160.1"
                     /translation="MPETKLEFSKELRDFIEEWKDKPGNLIMLLHRIQGEYTYIPREA
                     AEELSRLIGTPLAKIYGVITFYHYFKTTKPGKHKISICLGTACYLKGAQGLIDETESI
                     LGIKSEEVTDDGLFSIDPVRCLGCCGLAPVLMIGNDVYGKLTKEQLPEIIAKYRKL"
     gene            945..3035
                     /locus_tag="PLG14_03165"
     CDS             945..3035
                     /locus_tag="PLG14_03165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX48161.1"
                     /translation="MNRKSGLRALDATVAGIATVVAVALLLATGLSEGGGPPAVEGRV
                     RYESGTRALLLGGSWERYGGFVTDQDLAAEPRRPSFAASPLTPSILPGPAPEGGPVTY
                     RMRVELPLRGGRYSLFVPGLPGFRALLVDGKFLYDSSAGASPPRELSFEAQGPEVSIL
                     LQSGEGGPRLEAPGLLPGYVLFGEAKGLQVQRLVAAGLPAIGVGCFAVAALFFFLLFA
                     FWRANREFLAFSLFSLAAAFVALVSNAGILGLASPSAPAVERAYLAGLAALALAFFWF
                     LSSAFPKRLPLWAKAAFSVPPLLLAAAALLPLASASLAELPLLGPRPLYAAVFGLCAL
                     YCLAAFAWNLVLAAKGERRALWLLSGLAIAAAGAAARRAAPAGFNAAYFAEPLGFAAF
                     AFVAALMLVKRMGDGVESLAILTDYAADVSATVKRFIPKEFLDYLSKAEITDLRLGDH
                     VKKEMTIFFSDIRAFTELSERLTIEENFAFINSYLARVVPLITERGGFIDKYIGDAIM
                     ALFPGDTGPDAAIRAAVAMQEKVVEYNGHRAKMGYRPISMGVGIHTGDLMLGVVGVSD
                     RMENTVISDAVNLASRLQAITKAFNIGLAISEQSFKELEDPGSYKYRFIGKVKVKGKA
                     APVSVFEIFDGIAPALFERKMKVNTFFEQGMLSYYQKDFAGAMYYFKRVLDTVPEDGA
                     AAFYLENCMAKAAL"
     gene            3049..5049
                     /locus_tag="PLG14_03170"
     CDS             3049..5049
                     /locus_tag="PLG14_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOX48162.1"
                     /translation="MGERLSFGVDWYPEQWDESTWERDCERMRAQGVSVVRMMEFAWT
                     IVEPEKGRYDFSLFERAVEVAASRGLKVILGTPTATFPAWLLDEGEVLMTSPEGRVRD
                     FGARRMGCYNAPAYREAARKVAAEAAKRLGGHPAVEGWQVDNELGHEGSDRCVCGHCR
                     AAWHAWLERRYGKVEALNAQWGNIFWGTTYGRFDQVPVPRKQVSVGFNPGLLLDYDRF
                     MSDSAAAFVDAQVEALKAAIGPDRYVTTNLYPPPLSNAIDMERLTRDMDFASWDNYPI
                     WGAQDEPFPHHFQAFAQSYVRGLRGSGPFTVMEEFSGIQGHVCLGALPPEGQVALWTV
                     QAVARGANRIVYFRGRTAPYGQEQLCSGLLEADDGETSRSRVLAANAKRAKAELARLA
                     SVPFESDACLVYSKDDARVLREQYLSKGLYVKEAEWMQAGYDLELAKWFAPFAAFNVN
                     ADVKSAASVDVEKYALVSLPLYQMADPALVARLDAWVRRGGVLVLGYRSGARDLRNWN
                     IAAPLPGLFSELAGVRVPAFESLNLTRTRLRLKGLPALVPAHAEVWADHLEPAGAEPI
                     AWYSGRGKSCRGKPCATVNRVGRGKVFYIGTSPDPAAMFFLYRRILKEAGLSPRFLGK
                     EAEVVERRTDEGARVRIALNHSPRPRRILGRRLPPWGWAVLE"
     gene            5068..5601
                     /locus_tag="PLG14_03175"
     CDS             5068..5601
                     /locus_tag="PLG14_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48163.1"
                     /translation="MEDPSTLNDRGVALSDAGRYEEAIAAFDKALKLAPGNTGLVYNR
                     GEALRRAGRLAEARADLEAVLAAEGEEPDLLLALGLVAYEADDYDEAEERYERALVLK
                     ADFPQAWNDLGVVKFRRGDYQGARLAFEKAVSQKADYEEAWFNLKDSYDELGLESDKR
                     RAEKRLRELGGDEGESD"
     gene            5802..7682
                     /locus_tag="PLG14_03180"
     CDS             5802..7682
                     /locus_tag="PLG14_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOX48164.1"
                     /translation="MRKTNAGRIALAAVLVLAALSALGCASPYAKYAETPLNITFIET
                     TDVHGAIFPYNFIKAQEMKNSLAQVATLVASERAKEGSNVVLLDAGDTLQGQPTVYYS
                     NFVKTGATHVWSEAVNYLGYDALTVGNHDIEAGHPVYDKLYKEIKSPVVCANIVKEDG
                     TPYFTPYTVIKREGVKIAVLGMITPKIADWLPPQFWTGMQFVDMVETAKKWVPIIQEK
                     EKPDVLIGLFHSGVDYSYGGATKDTVMNENGSQLVAEQVPGFDMVFVGHDHMGWDGQG
                     WNVATKQKMEVKDPSGKTVYIYGALDAARKVPMVNLSLTWDPKAKGWIKKIEGKLVDM
                     ATVPADPAFTAKFQPAADEVKAWVDRPVGKLAAKITTRDSMFGDSAFVDLIHRIQLEL
                     SADPAMGLKKADVSFAAPLTFDATIPGSVDGTMYVRDMFNLYQYENFLYTMDMTGKQI
                     KDFLEYSYKSWFDTMPNEGNHLIAFQKDKDGKLITDARSGAYQTATRSYNYDSAAGIN
                     YTVDISKPAGSRIAISSMADGSAFDPAKTYVVAINSYRGQGGGGHLTTGAGLDKTAVQ
                     KLKFVNGATTKDLRFYLLTWFEKQKGAVTVAPIGNWKLVPEDLAAQGKATDYPILYPP
                     AK"
     gene            7925..8470
                     /locus_tag="PLG14_03185"
     CDS             7925..8470
                     /locus_tag="PLG14_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX48165.1"
                     /translation="MKGNRTYMDIGAILDEVFEAAKDFGDKINDEFGKPGCMPGFGPH
                     GPKTWFEMGQDENADYYPGYSYPPMNIYLTPERSIIFEFAVAGFDEKDISLTFQGDYM
                     VFSARIGLSANPDEGVRYIKRRLKLKDVEKQKYYVPADKFAQEEVKASFRNGILKVVV
                     PPKEETEASEGIRIEIVKEGE"
     gene            complement(8575..9039)
                     /locus_tag="PLG14_03190"
     CDS             complement(8575..9039)
                     /locus_tag="PLG14_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48166.1"
                     /translation="MRCETFRNRLAQADAGRGPGLFARAHLSRCAACRAYAELEAAAL
                     AAYRAPSPALPGLEDRIMAAVRLTPRPRRRVSLRDWVGVGLLIAVSTALIPLAEEFDW
                     VKELFGPSYALPLSLVLALVLTAYVAVFIGSHVTELLEFLERRLGHAIAPRA"
     gene            complement(9042..9599)
                     /locus_tag="PLG14_03195"
     CDS             complement(9042..9599)
                     /locus_tag="PLG14_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX48167.1"
                     /translation="MSGRLVADLSDEEVIGLVLGGDTEAFSQLVRRHQGRVLRLGMSF
                     FRDEEEAADFAQDVFVKAYTALASFKGRSKFSTWLLRIAYNTAINAKQRRKDAVLLDP
                     EAEIQGSPGVDESHLREETARAVRQAMAELPPRQALCIELYFYYDLKYAEIGEMTGFP
                     VNTIKSHVFRAKKLLRERLGEAKEA"
     gene            complement(9596..9934)
                     /locus_tag="PLG14_03200"
     CDS             complement(9596..9934)
                     /locus_tag="PLG14_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48168.1"
                     /translation="MSAHTVAAQVIISVIPIVGIVMGSVVVFFYLLWSHKEKKLMIER
                     GIYEPIRVDLDAFCLLAGLLLGLVGAVLSVLFALMDGAAYSLLGGLIPLALGAGLLAF
                     YRIRSRASAR"
     gene            complement(10112..10522)
                     /locus_tag="PLG14_03205"
     CDS             complement(10112..10522)
                     /locus_tag="PLG14_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOX48169.1"
                     /translation="MSQELICITCPMGCHLSVEKAEGGELSVSGNRCPRGAAYAREEL
                     FAPKRVVTATARAARACAGADSCAEAGRAQGLLDAPRRVPVRTAAAFPKERVGELLER
                     LYALEVELPVERGQVLVPDALGTGIDVIATRSIK"
     gene            complement(10532..11788)
                     /locus_tag="PLG14_03210"
     CDS             complement(10532..11788)
                     /locus_tag="PLG14_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48170.1"
                     /translation="MKEMRYDSVVIGGGAAGMAAALELAGRGLSCAVVEREGHLGGIL
                     TQCIHNGFGLIEFNEELSGPEFAERFEEKVRKTAITPFCGTTVIEMELEGDEKVLSCV
                     SSELGVVRLRAKAVVLAMGCRERNRGNIRIPGSRPAGVYTAGLAQRLVNIEGYVPGKD
                     IVIIGSGDIGLIMARRMSWVGCKVHAVVEILPYPSGLTRNIVQCLNDFGIPLYLSHLT
                     TDIYGKDRVEGVEITPIENGALMTEKAFKIPCDTVLLSVGLVPENELSKKMGVEINPM
                     TNGPYVDSSLMTSVPGVFAAGNVLHVHDLVDYVVEEARRAGAFAAEWLSGKRSAKEIR
                     VKAGPNVRYVNQSRVDPARDNKLYLRTLIVKNDAVLEVKLNNKVIRQARKSHVQPSEM
                     LSLSLGPADLEAALQAPDSTLEISIL"
     gene            complement(11785..13224)
                     /locus_tag="PLG14_03215"
     CDS             complement(11785..13224)
                     /locus_tag="PLG14_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX48171.1"
                     /translation="MKQGSDSFDVAVIGAGVSGANIARRLSAYELDVALVEKEADVSF
                     GTSKANSGIVHGGFHHNKKYLKARLELQGAMMFDRLKAELDFPFERCGIVVAAMHEDE
                     MRPIEQLYAQGVENGVIGIEMCSRERMLELEPKLSADCVGGLYAPGGGIVEPYRFVFS
                     LVENAEKNGVRVVKGFKVASARREGERWTVAAADGRTIKARYVVNAAGLYADEVSRIF
                     GGEEFEIKARKGEYYLLDRLTKCKPERVVFPVPTSVSKGMLVIPTVEGTVLIGPTATT
                     VPDKGDLSTTRVELDTILKSARNMIPSVSETDVITSFAGLRPCLEEDFYIEPSKKAPA
                     LIQVAGIQSPGLTASPAIGEYVKDLLKKEGLKLVEKADWDPFVRKVPRAKDLSPFELD
                     ELVKKDPAYGEIVCRCERVTEAEIVEAIRRGHVTLDGIKYFTRAQMGRCQGGFCTYKI
                     LKIIMRETGMGWDEITKRGEGSSVLRGEL"
     gene            13396..>13814
                     /locus_tag="PLG14_03220"
     CDS             13396..>13814
                     /locus_tag="PLG14_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48172.1"
                     /translation="MIQRRLLIRIREALASKRLVLVEGSPRSGKTTLAGQLVRAGSGA
                     ILLDARGPGAANLLRDPRFALAELGFGLRVLVLDNADEETAAALAGLAEAGGQPRLVL
                     FGPRFSDGLAAAPGVLRLSLGGLSLLEVGRANRARHWL"
BASE COUNT         2175 a         4917 c         4547 g         2175 t
ORIGIN      
        1 gcctggacga ggaagggaag tccgagcccc acctttcgcc gcgcgtgctt cttcccgtcc
       61 gtgtagaagg ggtcgagggc ccgcgcgagc tcctcctccg ccatgccgac gccgtcgtcg
      121 gcgacggcca cggagaagga ttcctccgtc tcggcgaagt cgatccgcac cgtcccggcg
      181 cccgactcga ccgcgttctg cgcgatgtcg aggacgtagt cgccgaggga gtagtgcaag
      241 cccgtccccg ccccggaggt cctagagctt gcggtacttg gcgatgatct ccggcagctg
      301 ctccttcgtg agcttgccgt agacgtcgtt gccgatcatg aggacgggcg cgaggccgca
      361 gcagcccagg cagcggaccg ggtcgatgct gaagaggccg tcgtccgtga cctcctcgct
      421 cttgatcccg aggatggact cggtctcgtc gatgaggccc tgggcaccct tgaggtagca
      481 ggcggtcccg aggcagatcg agatcttgtg cttgccgggc ttcgtggtct tgaagtagtg
      541 gtagaaggtg atgaccccgt agatcttcgc gaggggggtg ccgatgagcc tggaaagctc
      601 ctcggcggcc tcgcgcggga tgtaggtgta ctcgccctgg atgcggtgga gcaacatgat
      661 gaggttgccg ggcttgtcct tccactcctc gatgaagtcg cgcagctcct tcgagaactc
      721 gagtttcgtt tctggcatca aaagcctccc ttttgctgtt gaagctagat aaaattatat
      781 cgatattttg tttttggcag tatatactcc ttcctctcga ttgacaagag gagatggagc
      841 ttatttcgat acatgaaaca tagttaagtc gatttaactt accgtcaagc ccccaagcgc
      901 cggtttgatt tgtccagggc cgaccttgcg tgtatactgg tgccatgaat cgaaaaagtg
      961 gtctccgggc gctcgacgcg acggttgcgg gcatcgcgac cgtggtcgcc gtcgccctcc
     1021 tcctcgcgac gggcctttcc gagggcggcg gcccgccggc cgtcgagggc cgcgtccgct
     1081 acgagtccgg cacccgggcc ctcctcctgg gcggaagctg ggagcgctac ggcggcttcg
     1141 tcaccgacca ggacctcgcc gccgagccgc ggaggccctc cttcgccgcg agccccctca
     1201 ccccctccat cctgcccggg ccggcccccg aaggcggccc cgtcacctac cgcatgcggg
     1261 tggagctgcc gctgcgcggc gggcgctact cgctcttcgt ccccggcctc cccggcttcc
     1321 gggccctcct cgtggacgga aagttcctct acgactcctc cgccggcgcg agcccgcccc
     1381 gggagctctc cttcgaggcc cagggcccgg aggtctccat cctcctccag agcggagagg
     1441 gagggccccg cctcgaggct cccggcctcc ttccgggcta cgtactcttc ggcgaggcca
     1501 agggactgca ggtccagagg ctcgtcgcgg ccggcctccc cgcgatcggc gtcggctgct
     1561 tcgccgtcgc cgccctcttc ttcttcctcc tcttcgcctt ctggcgcgcc aaccgggaat
     1621 tcctcgcctt ctccctcttc tccctggccg cggccttcgt cgccctcgtc tccaacgcgg
     1681 ggatcctcgg cctcgcctcc ccctccgccc ccgccgtcga gcgcgcctac ctcgcgggcc
     1741 tcgcggccct cgccctcgcc ttcttctggt tcctctcctc cgccttcccg aagcgcctgc
     1801 ccctctgggc caaggcggcc ttctcggtcc cgcccctcct cctcgcggcc gcggccctcc
     1861 tccccctggc ctcggcctcc ctggcggagc tccccctcct cggcccgcgg ccgctctacg
     1921 ccgccgtctt cggcctctgc gccctctact gcctcgcggc cttcgcctgg aacctcgtcc
     1981 tcgcggccaa gggcgagagg cgcgcgctct ggctcctctc cggcctcgcg atagccgccg
     2041 ccggggccgc ggccaggcgg gccgcgccgg cgggcttcaa cgcggcctac ttcgccgagc
     2101 ccctgggctt cgccgccttc gccttcgtcg ccgccctcat gctcgtgaag cgcatgggcg
     2161 acggcgtcga gtccctcgcg atcctcaccg actacgcggc cgacgtctcc gcgacggtga
     2221 agcgcttcat ccccaaggaa ttcctagact acctctccaa ggcggagatc accgacttgc
     2281 ggctcggcga ccacgtgaag aaggagatga ccatcttctt ctccgacatc agggccttca
     2341 cggagctctc cgagcgcctc acgatcgagg agaacttcgc cttcatcaac tcctacctcg
     2401 cgcgggtcgt ccccctcatc accgagcgcg gcggcttcat cgacaagtac atcggcgacg
     2461 ccatcatggc cctcttcccc ggcgacaccg ggcccgacgc ggcgatccgc gccgccgtcg
     2521 cgatgcagga gaaggtcgtg gagtacaacg gacaccgcgc caagatgggc taccgcccca
     2581 tctccatggg cgtcggcatc cacacgggcg acctcatgct cggcgtcgtc ggcgtctccg
     2641 accggatgga gaacaccgtc atctcggacg cggtcaacct ggcctcccgc ctccaggcca
     2701 tcaccaaggc cttcaacatc ggcctcgcga tctcggagca gtccttcaag gagctcgagg
     2761 acccgggctc ctacaagtac cgcttcatcg gcaaggtgaa ggtgaagggc aaggccgccc
     2821 ccgtctccgt cttcgagatc ttcgacggca tcgcgccggc cctcttcgag cgcaagatga
     2881 aggtgaacac cttcttcgag cagggcatgc tctcctacta ccagaaggac ttcgccggcg
     2941 ccatgtacta cttcaagcgc gtgctggaca cggtgcccga ggacggggcc gcggctttct
     3001 acctggagaa ctgcatggcg aaggccgcgc tgtagcgcag gaggcggcat gggcgagagg
     3061 ctcagtttcg gcgtcgactg gtatcccgag cagtgggacg agtccacctg ggagcgcgac
     3121 tgcgagcgca tgcgcgccca gggcgtctcc gtcgtgcgga tgatggagtt cgcctggacg
     3181 atagtcgagc ccgagaaggg gagatacgac ttctccctct tcgagcgggc ggtggaggtc
     3241 gcggcgagcc gcggcctcaa ggtgatcctg ggcacgccga ccgcgacctt ccccgcctgg
     3301 ctcctcgacg agggcgaggt cctcatgacc tcgcccgagg gccgcgtccg cgacttcggc
     3361 gcgaggcgca tgggctgcta caacgccccc gcctaccgcg aggccgcccg caaggtcgcg
     3421 gcggaggcgg cgaagcgcct gggcggccac cccgccgtgg agggctggca ggtcgacaac
     3481 gagctcggcc acgagggctc ggaccgctgc gtctgcgggc attgccgggc cgcctggcac
     3541 gcctggctcg agcggcgcta cggcaaggtc gaggccctga acgcccagtg gggcaacatc
     3601 ttctggggga cgacctacgg ccgcttcgac caggtgcccg tcccccgcaa gcaggtctcc
     3661 gtaggcttca acccgggcct cctcctcgac tacgaccgct tcatgtccga ctccgccgcc
     3721 gccttcgtcg acgcccaggt ggaggccctc aaggcggcga taggccccga ccgctacgtc
     3781 accacgaacc tctacccgcc gcccctctcc aacgcgatcg acatggagcg cctgacccgg
     3841 gacatggatt tcgcctcctg ggacaactac ccgatctggg gcgcgcagga cgagcccttc
     3901 ccccaccact tccaggcctt cgcgcagagc tacgtccgcg gcctccgcgg ctccgggccc
     3961 ttcacggtca tggaggagtt ctccggcatc cagggccacg tctgcctcgg cgccttgccg
     4021 cctgagggcc aggtcgccct ctggaccgtc caggccgtgg cccgcggggc caaccgaatc
     4081 gtgtatttca gggggaggac cgcgccctac ggccaggagc agctctgctc cggactcctc
     4141 gaggccgacg acggggagac ctcgcgctcg cgggtcctcg cggccaacgc gaagcgcgcc
     4201 aaggccgagc tcgcccgcct cgcctccgtc cccttcgaga gcgacgcctg cctcgtctac
     4261 tcgaaggacg acgcgagggt cctgcgcgag cagtacctct ccaagggcct ctacgtcaag
     4321 gaagccgagt ggatgcaggc gggctacgac ctcgagctcg ccaagtggtt cgcgcccttc
     4381 gccgctttca acgtgaacgc ggacgtgaag agcgccgcct ccgtcgacgt ggagaagtac
     4441 gccctcgtct ccctgccgct ctaccagatg gccgacccgg ccctggtcgc gcgcctggac
     4501 gcctgggtgc ggaggggcgg cgtcctcgtc ctgggctacc gctcgggcgc gcgggacctg
     4561 aggaactgga acatcgccgc ccccctaccc ggcctcttct ccgagctcgc gggcgttcgc
     4621 gtgccggcct tcgagtcgct caacctcacc cgtacgcgcc tgaggctcaa gggcctgccc
     4681 gccctcgtcc ccgcccatgc cgaggtctgg gcggaccacc tggagcccgc gggcgccgag
     4741 ccgatagcct ggtactccgg gcgcgggaag tcctgccgcg gcaagccctg cgcgacggtg
     4801 aaccgcgtcg gcaggggcaa ggtcttctat atcgggacga gccccgaccc agcggccatg
     4861 ttcttcctct accgccgcat cctcaaggag gccggcctct cgccccgctt cctgggcaag
     4921 gaggccgagg tcgtcgagcg gcggacggac gagggagccc gcgtgcgtat agccctgaac
     4981 cactcgccca ggcccaggcg gatcctcggg aggcggctgc cgccctgggg ctgggcggtg
     5041 ctcgagtagg cgggctatac tcgaggcatg gaagacccca gcaccctcaa cgaccgcggc
     5101 gtggccctct ccgacgccgg ccgctacgag gaggccatcg cggccttcga caaggccctg
     5161 aagctcgccc ccggcaacac gggcctggtc tacaaccgcg gcgaggccct gcgccgcgcc
     5221 ggccgcctgg ccgaggcccg ggccgacctg gaggccgtcc tggccgccga gggagaggag
     5281 cccgacctcc tcctggccct cggcctcgtc gcctacgagg ccgacgacta cgacgaggcg
     5341 gaggagcgct acgagcgcgc cctggtcctc aaggccgact tcccccaggc ctggaacgac
     5401 ctgggcgtcg tgaagttccg gcgcggcgac taccagggcg cccgcctggc cttcgagaag
     5461 gcagtctccc agaaggccga ctacgaggaa gcctggttca acctcaagga cagctacgac
     5521 gagctcggcc tcgagtccga caagcgcagg gccgagaagc gcctccgcga gctcggcggc
     5581 gacgagggcg agagcgacta acccgcggcc ggccgcgcgg cttccggccc gcccgcgggc
     5641 cggaggccgc cgcgccgccc ctgccccgtt ccgcgctggc gggaccgccg cgcggggccg
     5701 ccctccccgg ccaaaagacg cgcccctcct tcacagatgc ccgatgcggg tatagaatga
     5761 tcttccccct agcggggaaa gtcattaaca ggaggaatcc catgcgcaag accaacgccg
     5821 gacggatcgc gctcgccgcc gtcctcgtcc tcgccgcgct ctccgccctc ggctgcgcct
     5881 cgccctacgc gaagtacgcc gagacgccgc tcaacatcac cttcatcgag acgaccgacg
     5941 tccacggggc catcttcccc tacaacttca tcaaggccca ggagatgaag aactccctgg
     6001 cccaggtcgc gaccctcgtc gcgtccgagc gcgccaagga gggctcgaac gtcgtcctcc
     6061 tggacgcggg cgacaccctc cagggccagc ccaccgtcta ctactcgaac ttcgtgaaga
     6121 ccggcgcgac ccacgtctgg tccgaggcgg tcaactacct cggctacgac gccctcaccg
     6181 tgggcaacca cgacatcgag gccgggcacc ccgtctacga caagctctac aaggagatca
     6241 agtctcccgt cgtctgcgcc aacatcgtca aggaagacgg cacgccctac ttcaccccct
     6301 acacggtcat caagcgcgag ggcgtcaaga tcgccgtcct gggcatgatc acgcccaaga
     6361 tcgccgactg gctcccgccc cagttctgga ccgggatgca gttcgtcgac atggtcgaga
     6421 ccgcgaagaa gtgggtcccc atcatccagg agaaggagaa gcccgacgtc ctcatcggcc
     6481 tcttccactc cggcgtggac tactcctacg gcggggccac caaggacacc gtcatgaacg
     6541 agaacggcag ccagctcgtc gccgagcagg tgccgggctt cgacatggtc ttcgtcggcc
     6601 acgaccacat gggctgggac ggccagggct ggaacgtcgc taccaagcag aagatggagg
     6661 tcaaggaccc ctccggcaag accgtctata tctacggcgc cctggacgcc gcgcggaagg
     6721 tccccatggt caacctctcc ctcacctggg accccaaggc caagggctgg atcaagaaga
     6781 tcgagggcaa gctcgtcgac atggcgaccg tccccgccga tcccgccttc accgccaagt
     6841 tccagcccgc cgccgacgag gtcaaggcct gggtcgacag gccggtcggc aagctcgccg
     6901 ccaagatcac cacccgcgac tccatgttcg gcgactccgc cttcgtggac ctcatccacc
     6961 gcatccagct cgagctctcc gccgacccgg ccatgggcct caagaaggcc gacgtctcct
     7021 tcgccgcgcc cctcaccttc gacgcgacca tccccggcag cgtcgacggc acgatgtacg
     7081 tccgcgacat gttcaacctc taccagtacg agaacttcct ctacacgatg gacatgaccg
     7141 gcaagcagat caaggacttc ctggagtact cctacaagtc ctggttcgac acgatgccca
     7201 acgagggcaa ccacctcatc gccttccaga aggacaagga cgggaagctc atcaccgacg
     7261 cccgctccgg cgcctaccag accgcgaccc gctcctacaa ctacgactcc gccgccggca
     7321 tcaactacac ggtcgacatc tccaagcccg cgggctcccg gatcgcgatc tcctcgatgg
     7381 ccgacggctc cgccttcgac cccgcgaaga cctacgtcgt cgcgatcaac tcctaccgcg
     7441 gccagggcgg cggcggccac ctcacgaccg gcgccggcct cgacaagacc gccgtccaga
     7501 agctcaagtt cgtgaacggc gccacgacca aggacctccg cttctacctc ctcacctggt
     7561 tcgagaagca gaagggcgcg gtcaccgtgg cccccatcgg caactggaag ctcgtccccg
     7621 aggacctggc cgcccagggc aaggcgacgg actacccgat cctctacccg cccgcgaagt
     7681 aggcggcgcc ccgctccgga ctggctccgg ggcggcgttc aaaaaggggg gcggggccgc
     7741 aaggctccgc cccccccttt ttgcgcttgc cgcgacgggc caagggcgat accttatagg
     7801 cagggagagg aaagcccgag ctggttcctc ccaccgccgt cgcccctttc gggggacggg
     7861 accaagcaag accgaaggcg cgcaggggca gcctccggcc atctcgctcc tataggaggg
     7921 gactatgaaa ggcaaccgca cctacatgga catcggggcc atcctggacg aggtcttcga
     7981 ggccgcgaag gacttcggcg acaagatcaa cgacgagttc ggcaagcccg gctgcatgcc
     8041 cggcttcggc ccccacgggc ccaagacctg gttcgagatg ggccaggacg aaaacgccga
     8101 ctactacccc ggctattcct acccgccgat gaacatctac ctcacgcccg agcggagcat
     8161 catcttcgag ttcgccgtgg cgggcttcga cgagaaggac atcagcctca ccttccaggg
     8221 cgactacatg gtcttctccg cccggatcgg cctctccgcc aatcccgacg agggcgtccg
     8281 ctacatcaag cgccgcctca agctcaagga cgtggagaag cagaagtact acgtccccgc
     8341 ggacaagttc gcccaggaag aggtcaaggc cagcttccgg aacggcatcc tcaaggtcgt
     8401 cgtgcctccc aaggaggaga ccgaggccag cgagggaatc cgcatcgaga tcgtgaagga
     8461 aggcgagtaa gtcgcccggg cccgccgaca gggcccgaag caagagaggc cgtccgggcg
     8521 tcccggacgg cctctccgtt tttgcgccgg caaacgccgc tcccgggaag ggcctcaggc
     8581 gcggggcgct atcgcgtggc ccagcctgcg ctccaggaat tccaggagct ccgtcacgtg
     8641 gcttccgatg aagacggcga cgtaggcggt gaggaccagg gccaggacga gggagagggg
     8701 caaggcgtag ctcggcccga aaagctcctt cacccagtcg aattcctcgg cgagggggat
     8761 gagggcggtc gagacggcga tgaggaggcc gacgccgacc cagtcgcgaa ggctcacccg
     8821 gcggcgaggc cgcggcgtga ggcgcacggc cgccatgatc ctgtcctcga ggcccggcag
     8881 ggcgggagac ggcgcgcgat aggccgcgag cgcggccgcc tcgagctcgg cgtaggcccg
     8941 gcaggcggcg cagcgcgaaa ggtgggccct cgcgaagagg cccgggccgc gcccggcgtc
     9001 ggcctgggct aggcggttcc tgaaggtctc gcaacgcatg atcaagcctc cttggcctct
     9061 ccgagccgct ccctgaggag cttcttggcg cggaagacgt gcgatttgat ggtgttcacc
     9121 ggaaatcccg tcatctcgcc gatctcggcg tacttgaggt catagtagaa atagagctcg
     9181 atgcagagcg cctgccgggg cgggagctca gccatcgcct ggcgcacggc ccgggccgtc
     9241 tcctcccgca ggtgggactc gtccacgccc ggggagccct ggatctcggc ctcgggatcg
     9301 aggaggacgg cgtccttcct gcgctgcttc gcgttgatcg ccgtgttgta ggcgatgcgc
     9361 aggagccaag tcgagaattt cgagcggccc ttgaaggagg ccagggccgt ataggccttc
     9421 acgaagacgt cctgggcgaa gtcggcggcc tcctcctcgt cgcggaaaaa actcatcccg
     9481 aggcgcagga cccgcccctg gtgcctccgc acgagctgcg agaaggcttc ggtgtcgccg
     9541 ccgaggacga ggccgatgac ctcctcgtcg gaaaggtcgg cgacgagccg accgctcatc
     9601 gggcggaagc gcggctccgg atccggtaga aggcgaggag gccggcgccc agggcgaggg
     9661 ggatgaggcc gccgaggagg ctgtaggcgg cgccgtccat gagggcgaag aggacggaga
     9721 gcacggcgcc gaccaggccg aggaggaggc ccgcgaggag gcagaaggcg tccaggtcga
     9781 cgcggatggg ctcgtagatg ccccgctcga tcatgagctt cttctccttg tggctccaca
     9841 ggagatagaa gaagaccacc acgctcccca tcacgatccc gacgataggg atgacggaga
     9901 tgatgacctg ggcggctacg gtatgggcgg acatcggagc acctcttgtt cgaggaaatt
     9961 gcgcggcctc ggggccgcct gcctctagtt ggacacggga acggcctttg ggttgcgcgt
    10021 ccgccggcgc cgagcgccgg cggcgcgatc acgtcgatgc ccgcgcgggc gcggggcgct
    10081 tccgcgatcc ccggggccct cgggacgggc cttacttgat cgaacgggta gctatgacgt
    10141 cgatacccgt cccgagggcg tcggggacga ggacttggcc gcgctcgacg gggagctcga
    10201 cctccagggc gtagagccgc tcgaggagct cgccgacgcg ctccttgggg aaggcggcgg
    10261 cggtccggac cgggacgcgg cggggagcgt cgaggaggcc ctgggcgcgg ccggcctccg
    10321 cgcaggaatc cgcgccggcg caggccctcg cggccctggc cgtggcggtc acgacgcgct
    10381 tcggggcgaa gagctcctcg cgggcgtagg ccgcgccgcg ggggcagcgg ttgccgctca
    10441 cggagagctc gccgccctcg gccttctcga cggagaggtg gcagcccatc gggcaggtga
    10501 tgcagatcag ctcctggctc atgcgctcct cctataggat cgagatctcg agcgtcgagt
    10561 ccggcgcctg gagcgcggcc tcgaggtcgg cgggccccag ggagaggctg agcatctccg
    10621 agggctggac gtggctcttc ctggcctggc ggatgacctt gttgttgagc ttcacctcca
    10681 ggacggcgtc gttcttcacg atgagggtgc ggaggtagag cttgttgtcg cgcgcggggt
    10741 cgaccctgga ctggttcacg tagcggacgt tcgggcccgc cttcacgcgg atctccttcg
    10801 cggagcgctt gccggagagc cactcggcgg cgaaggcccc ggcccggcgc gcctcctcca
    10861 cgacgtagtc cacgaggtcg tggacgtgga ggacgttgcc agcggcgaag acgccgggga
    10921 cgctggtcat gagggaggag tcgacgtagg gcccgttcgt catcgggttg atctcgacgc
    10981 ccatcttctt ggagagctcg ttctccggca cgaggccgac ggagaggagg accgtgtcgc
    11041 aggggatctt gaaggccttc tccgtcatga gggcgccgtt ctcgatcggc gtgatctcca
    11101 ccccctcgac cctgtccttc ccgtagatgt cggtggtgag gtgggagagg tagaggggga
    11161 tgccgaagtc gttcaggcac tggacgatgt tccgcgtgag gccggagggg tagggcagga
    11221 tctccacgac ggcgtggacc ttgcagccga cccagctcat gcggcgggcc atgatgaggc
    11281 cgatgtcgcc cgagccgatg atgacgatgt ccttgcccgg cacgtagccc tcgatgttga
    11341 cgaggcgctg ggccaggccc gcggtgtaga cgccggccgg ccgggagccg gggatgcgga
    11401 tgttgcctcg gttgcgctcg cggcagccca tcgccaggac cacggccttg gcgcggaggc
    11461 ggacgacgcc gagctcgctg gagacgcagg aaaggacctt ctcgtccccc tccagctcca
    11521 tctcgatcac ggtcgtgccg cagaagggcg tgatcgccgt cttccgcacc ttctcctcga
    11581 agcgctccgc gaactcgggg ccggagagct cctcgttgaa ctcgatgagg ccgaagccgt
    11641 tgtggatgca ctgggtcagg atgccgccca ggtgcccctc gcgctccacg accgcgcagg
    11701 agagcccgcg ccccgcgagc tccagggccg cggccatgcc cgcggcgccg ccgccgatga
    11761 cgacggaatc gtagcgcatc tctttcatag ctcgcccctc aagacggagc tgccctcgcc
    11821 ccgcttcgtg atctcgtccc agcccatgcc ggtctcgcgc atgatgatct tcaggatctt
    11881 gtaggtgcag aagccgccct ggcagcggcc catctgcgcc cgcgtgaagt acttgatgcc
    11941 gtccagggtc acgtggccgc ggcggatcgc ctcgacgatc tccgcctcgg tgacgcgctc
    12001 gcagcggcag acgatctcgc cgtaggcggg gtctttcttc acgagctcgt cgagctcgaa
    12061 gggggagagg tccttggccc gcggcacctt gcggacgaag gggtcccagt cggccttctc
    12121 cacgagcttc aggccctcct tcttgaggag gtccttcacg tactcgccga tcgcggggct
    12181 cgccgtgagg cccggggact ggatccccgc gacctggatc agggcggggg ccttcttgga
    12241 gggctcgatg tagaagtcct cctccaggca ggggcggagg cccgcgaagc tcgtgatcac
    12301 gtccgtctcg gagaccgagg ggatcatgtt gcgcgcgctc ttgaggatcg tgtcgagctc
    12361 gacgcgggtc gtcgagaggt ctcccttgtc ggggaccgtc gtcgccgtcg gcccgatgag
    12421 gaccgtgccc tcgacggtgg ggatgacgag catgcccttg gagacggagg tgggcacggg
    12481 gaagacgacc cgctccggct tgcacttcgt caggcggtcc aggaggtagt actcgccctt
    12541 gcgcgccttg atctcgaact cctcgccgcc gaagatccgg gagacttcgt cggcgtagag
    12601 gccggcggcg ttgacgacgt agcgggcctt gatcgtccgc ccgtcggccg cggccacggt
    12661 ccagcgctcg ccctcgcgcc tcgcggaggc gaccttgaag cccttgacga cgcggacgcc
    12721 gttcttctcc gcgttctcca cgagtgagaa gacgaagcgg tagggctcga cgatgccgcc
    12781 gccgggggcg tagaggccgc ccacgcagtc ggcggagagc ttgggctcga gctcgagcat
    12841 ccgctcgcgg gagcacatct cgatgccgat gacgccgttc tcgacgccct gggcgtagag
    12901 ctgctcgatg ggccgcatct cgtcctcgtg catcgccgcg acgacgatgc cgcagcgctc
    12961 gaaggggaag tcgagctcgg ccttgaggcg gtcgaacatc atcgcgccct ggagctcgag
    13021 ccgggccttc aggtacttct tgttgtggtg gaagccgccg tgcacgatgc cggagttggc
    13081 cttggaggtg ccgaaggaga cgtcggcctc cttctccacg agggcgacgt cgagctcgta
    13141 ggccgagagc ctgcgcgcga tgttcgcccc gctcaccccc gcgccgatga cggcgacgtc
    13201 gaaggaatcg gaaccttgtt tcacttcgtc tctcccctgc cggccttcgc cttcgcgcgc
    13261 ccttggggcg cgggagccgg ccgaatgcga ccttggggac agtatagggc aggcgacagg
    13321 aagcggaagg cggttccatg gggaattcag gctcgacagc ctgcaatttg aacgctatca
    13381 tttcaaatta cggtgttgat ccagagacga ctcctcatca ggatacgcga agccctcgcc
    13441 tcgaagcgcc tcgtcctcgt cgagggctcg cctcgctcgg ggaagaccac cctcgccggc
    13501 cagctcgtcc gcgcgggctc gggcgccatc ctcctggacg cgaggggccc cggcgcggcg
    13561 aacctcctcc gcgacccccg cttcgccctc gccgagctcg gcttcggcct ccgcgtcctc
    13621 gtcctggaca acgccgacga ggagacggcg gcggcgctcg cgggcctcgc cgaggcgggc
    13681 ggccaacccc gcctcgtcct cttcggaccg cgcttttcgg acggcctcgc cgccgcgccc
    13741 ggggtcttgc ggctctccct gggcggcctc tccctcctcg aggtgggccg ggcgaaccgc
    13801 gcgcggcact ggct
//
LOCUS       DAOOSJ010000037        13721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_2991_length_13721_cov_4.959974, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000037 DAOOSJ010000000
VERSION     DAOOSJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_13721_cov_4.959974"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLG14_03225"
     CDS             <1..441
                     /locus_tag="PLG14_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48173.1"
                     /translation="DEIDAALDEQNVIRFVNLLREFGRSSQFVVITHNKKTVTGADAL
                     LGVTMEESGVTKVIAVRLANPEGAEAKVPEPLTPMDEEEVAYESGVELPPDEPSRGAP
                     AAGAVQGGAAVAAGEAGEPAPAVELAEAAAGGAASGATGGGGEA"
     gene            445..1005
                     /locus_tag="PLG14_03230"
     CDS             445..1005
                     /locus_tag="PLG14_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48174.1"
                     /translation="MALDPKAILSKLPSLLPGLAKRLPRRPGKASEKGSLEPFSSIED
                     ETPGADLSLEKNAAIAFKRKRERIDLREAAAAFFGNRTALLSTAAVLLVLLLLATAAF
                     IAGRPPKPLPAAAAVDPEGEALVGKFIIPPETVLEPRVELDREPKRVYTEADAARFRT
                     DPAGVDIQALAARNDAAAEALFGTVP"
     gene            1035..2666
                     /locus_tag="PLG14_03235"
     CDS             1035..2666
                     /locus_tag="PLG14_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48175.1"
                     /translation="MRLLRALAFAVLAAAALAACASAPASPKAGDAASPSAGAAPEAA
                     PPAPGAEGQAAPAAADAPSGPAGSEATAAPDSTSAAAVPSETAPASAPGAGDAKGQAA
                     AKPELSESRRLPFRLSLLLLNLPDPAQPAGLAAPKAVAAAAPAAVPAPAAKPAPVALP
                     EPAKPAPAKPAAAPAAKAAVPEKTPAAQPKAAAPAPAPAAKPAPAKAAPTLPPAPPSI
                     LPLTDPLAEKAVAKSAEIMRKVEAEQGLRIEIPFEGTGWTYLGERSGSEGIVYESRRF
                     EGSGLVFALGAQKVGDYVLRFQRQDLLRGIVYDELVGVTVRPRPAVRAAPAADPTPVS
                     PASSAAAAGVPPSASPAIATPSGLPAGPAAAATAGGAAIAGTGGVMTSGALAGGALAT
                     GAGTGAAATAGGAMPGVTAGAGAAATGGAMTSGAATAGPGAAANPALPAAAAALAPDS
                     PEGLLYLARSELGAGRVRGALDALDRFLSLYPAGMDEVYLLYGLAHEQNGPFRDIKKA
                     YAEYKKLAADYPQSPFWDRAAERIAYIERHYLEIR"
     gene            2790..>3988
                     /locus_tag="PLG14_03240"
     CDS             2790..>3988
                     /locus_tag="PLG14_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48176.1"
                     /translation="MKRMILCAALLCAAASAAFAQTANAKAPAAEAARVEGRRYVVYS
                     ELGGVAAEGLAARLDALFELYGAFFRFDPALLQGKLVVREFAGKAAFDSYLAQVAGQG
                     KEDFVYLHYQSPERRELLVFAKDEPDRSASLAHQAFVQYIKAFVQNPPLWLRDGFAVF
                     FETAAWDEEKGSLSFSENLAWLETAKALAAKGSFLPLERLLSITPEESRSSLDVYYPE
                     AWAFVSFLANGAEGGYDRFLWDTISGLRKDASLQENQDAAAARLEAWYGKSDSEKAFA
                     SYLEGRLTFPELVQAGVKSYGEKAYDAAREAFEAARELDPESYVPPYYLGLIAYAKGD
                     FGLADFEYKDALRLGCDPAIGNYALGLNAYAQNRAEDAKGFLGLAKEAAPERYGAKVE
                     ELLARFQ"
     assembly_gap    3989..4088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4119..4382)
                     /locus_tag="PLG14_03245"
     CDS             complement(4119..4382)
                     /locus_tag="PLG14_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48177.1"
                     /translation="MSPFEIGMLLCFGAAWPLSIAKSWKARSTKGKSLFFLIVILVGY
                     ACGILNKLLVRYDGVLWLYVLNGLMVATDVALWFRNRRLEGQA"
     gene            complement(4492..4929)
                     /locus_tag="PLG14_03250"
     CDS             complement(4492..4929)
                     /locus_tag="PLG14_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48178.1"
                     /translation="MIAKSDMPNINEKRPDGIKSDGGSYRVLIVDDSMFIAKQLGQIL
                     TSEGFEVVGTAGDGLAGIEKYKELCPNVDLVTMDITMPKMDGVTALEKILEFDKNATI
                     VMVSALGKEDVVKKSLLLGAKSYIVKPLDRKKVLERILAVLKH"
     gene            complement(5192..5926)
                     /gene="ribA"
                     /locus_tag="PLG14_03255"
     CDS             complement(5192..5926)
                     /gene="ribA"
                     /locus_tag="PLG14_03255"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOX48179.1"
                     /translation="MERLTRACLTADCDKALGREELARKLEGEPDFPLEERYCKGKGA
                     YCADGDFCVELVAHAHLPTRFGDFELYGFYDAREDKEHTALVRGDVAGAEGLPVRVHS
                     ECHTGDVLGSLRCDCRDQLEAALRQVGALPRGAVLYLRQEGRGIGLLNKIKAYRLQEL
                     GLDTVEANRYLGFPDEGRDYKVAAKMLALLGVSSIALLTNNPDKIDKLRAEGVKVTER
                     LPLVMPPNPHDEGYLAAKRDRMGHLI"
     gene            6026..6985
                     /locus_tag="PLG14_03260"
     CDS             6026..6985
                     /locus_tag="PLG14_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX48180.1"
                     /translation="MLESLLPVLPLVLLLLAWLAEAFLLLAAAMALLSHRLMVGISRL
                     DGKTIAARLLSKGEFGPEALELPWIRERIASPHGYELAVHGLKGEGDRLAIFQHGVGW
                     NWMGVLKYMEILRREGWSVAALDSRGHGASGGKDPSYGFFEKGDLLALADWALARFPS
                     SGGFAAFGLSLGAASVLQYAPLDPRLDAVVADCPFSSAAEELDHRLKRSFAPFFLRPL
                     VVRLMDLLCRRLDGFSLYAASPERAILETDKPVLLVHGREDDYVPWRMSARMARERRR
                     LLPAARTELLLVEGATHARSHKAAPEAYERALLGFLDSAGRPC"
     gene            7214..8536
                     /gene="hflX"
                     /locus_tag="PLG14_03265"
     CDS             7214..8536
                     /gene="hflX"
                     /locus_tag="PLG14_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOX48181.1"
                     /translation="MNELKDTAIRPERAFLVGVQTDKISKAEAESLLKELGSLASTLG
                     VEKAGSLLVKLRDRTAALLLGTGKADEVVAAAKAEGADSILFDHALSPVQQRNWEARS
                     DLKVYDRSELIIKIFASRALTREAGLQVELAELEFSLPRLAHSYEALSRQRGGRYGTK
                     GSGEQKIELDRREIEKRIHEIKEELKTARKSREVQRKRRERQTLPRAAVVGYTNAGKS
                     SLLNALTRASVHVEDKLFATLDPTTRRIALAKGGALLLTDTVGFVRNLPHGLVEAFKA
                     TLEEAAAADLLVHVVDASDPEAERHMATTDAVLREIGAGEVPRVVVLNKVDLLEDRSL
                     LSGLAAGHEGAKAVSVKTGEGIPELLAAIEEGLTARAGEVELRIPDSDYAFVALLHRE
                     ASILEESRDDEGTILRCRLPDRLQSRFEPYRTDSGASGAASGREAAGE"
     gene            complement(8744..10312)
                     /locus_tag="PLG14_03270"
     CDS             complement(8744..10312)
                     /locus_tag="PLG14_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOX48182.1"
                     /translation="MNSKASPDRDAARGGENLIQRLLSFFAGMGDPDAEKRKILKAIA
                     KDLSRSRFKFYRPKGQEALPGLARFFYEIYKVTAPAQVLLGNAASSSALRSFVIDSFL
                     TKDQKELSERLTEAHILERAKSLGLRELQAEVKDDLSSFYAVFDGDRIREIDSAYNTL
                     LSFINFVNFDYYFLLKKYDSSLPERSFGRAPKFEAINGDYVADDIEDFLEIFLPLDLE
                     ADWKRIFQALHDYKGAEIIQHDAWQKLLPAVAEVRKSQVLELVVKHIKKTPAWAAQPR
                     YPNERIVEPFLQKLKAQLETLLQRLVQEKRNAKIDEIAKQVFGTSVVLRMKNYTEKAN
                     VIFAKKMLGGYTQAPALNYLKAYLMDYFKKDIRELVDLLIIRGQWTTIVQSQQLSDGY
                     HGLLEASEAILAFDESLADEGEIGTRMRSALAKSERDKEQMKYLRTMLKDINEKALGI
                     VTKAAVNLIVVGRYLKTLIEDYDRSHHEILINWKEIEGASSRPIKEWLVEVYKKIYFM
                     VQLLQFYAKEEPQP"
     gene            complement(10318..11124)
                     /locus_tag="PLG14_03275"
     CDS             complement(10318..11124)
                     /locus_tag="PLG14_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOX48183.1"
                     /translation="MEQLEIRNAFPGAAVFRLRETTSTMDEARRLVRMGFPPGTTVVA
                     DAQSSGRGRLPERRWASPPGENLLSTVILGPEAAKLPGFTLRVGLALCRTAAVFAVRR
                     GGSLSSPPALKWPNDVLVGGRKLAGVLCEAGPEATLVGFGLNVNQLGFPPELEGKATS
                     LALELGLPGGLGLPELLELALGELAFVLADGDWRQEAEERLWRRGEEAAFLAGSPEGG
                     ETVTGVLEGLAPSGALLLRPAGADRPLEFASGELLFRAPRVDRSGSHHIR"
     assembly_gap    11173..11272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11317..13176
                     /gene="glmS"
                     /locus_tag="PLG14_03280"
     CDS             11317..13176
                     /gene="glmS"
                     /locus_tag="PLG14_03280"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOX48184.1"
                     /translation="MCGIVGYAGPRRATPILLEGLKRLEYRGYDSAGIAVGQVEGEAP
                     GSQAGRGGRLAVVKKTGKIAALRSEVPPELPGSWGIGHTRWATHGGVTDANAHPHLDA
                     SGKIAVVHNGIVENYRSLRSMLEKEGVRFASETDSEVIPHLVAKYYSGDLEAAVKEAI
                     ARLEGTYAVAVIHADEPGRIVGARNGGPLVVGVGDGEMFLASDVNAMIAHTKEVVYLS
                     DGELVVLDPEGFRTTDRQDRPVAKKVDQVSWEAGAIEKGDFACYMEKEIFEQPESIDR
                     ALGGRLEADSATAKLGGLNLDRRQLRQVGRVRVIAAGTSWHAGLVGAQLLESLARIPA
                     GAELASELRYRNPVVERDSLWFAVSQSGETADTLYAMREIQRKGGTVLGVCNVVGSTV
                     ARESDGGIYVHSGPEIAVASTKAFTSQLAAFYLFTLLMARMRDLSQADGARFAEALAG
                     VPAKVEETLRCRGAVQAAAKKYARATDFLFLGRGLMYPIALEGALKLKEISYVHAEGY
                     AAGEIKHGPIALVSPETPSVFLVPDDALREKTISNMREIKARGGPVIAVGVEGDDEVA
                     AIADDFIATPRMDPRFYPFTMVVPLQLFAYFTALELGRDVDQPRNLAKSVTVE"
     gene            13176..>13721
                     /locus_tag="PLG14_03285"
     CDS             13176..>13721
                     /locus_tag="PLG14_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48185.1"
                     /translation="MPRRAGRRAAARGALPAPFILLAFLAASGLAVQAAAQAAGGGAI
                     TGGAARGGAAAARPELPPGRALLLRGPEPRKGLPFFSPNAIPALVGEYEVSKSLVRVW
                     SSRSPLSFGKSWESIPIKLPASLRALRLARDGGPVIALAGPDYSLFFELPADTPAFRS
                     FLAAFERRFAFFFEGAPTDAEL"
BASE COUNT         1921 a         4929 c         4625 g         2046 t
ORIGIN      
        1 gacgagatcg acgcggccct ggacgagcag aacgtcatcc gcttcgtgaa cctcctccgg
       61 gagttcggcc gctcgagcca gttcgtcgtc atcacgcaca acaagaagac ggtgacgggc
      121 gccgacgccc tcctgggcgt cacgatggag gagtcgggcg tcaccaaggt gatcgccgtc
      181 cgcctcgcga atcccgaggg cgcggaggcc aaggtgcccg agcccctcac gccgatggac
      241 gaggaggagg tcgcctacga gtcgggcgtc gagctcccgc ccgacgagcc ttcccggggc
      301 gcccccgcgg ccggcgccgt ccagggcgga gcggccgtcg ctgccggcga ggccggtgag
      361 cccgctcccg ccgtcgagct ggccgaggcc gcggcgggcg gcgccgcaag cggcgcgacg
      421 ggcgggggcg gggaggccta ggccatggcc ctcgatccca aggccatcct ttcgaagctc
      481 ccctccctcc tcccggggct cgcgaagcgc ctcccgcggc gtcccgggaa ggcttcggag
      541 aaggggagcc tcgagccctt ctcgagcatc gaggacgaga ccccaggagc cgacctctcc
      601 ctcgagaaga acgccgcgat cgcgttcaag aggaagcgcg agcgcatcga cctgcgcgag
      661 gccgccgcgg ccttcttcgg gaacaggacg gccctccttt cgaccgccgc cgtcctcctc
      721 gtcctcctcc tcctcgccac cgccgccttc atcgccggcc gtcccccgaa gccgcttccc
      781 gcggcggccg ccgtcgaccc ggagggcgag gccctcgtgg gcaagttcat catccctccg
      841 gagacggtcc tcgagccgag ggtcgagctg gatcgcgagc ccaagcgggt gtatactgag
      901 gccgacgccg cccgtttccg caccgatccg gccggcgtcg acatccaggc cctcgccgcg
      961 cggaacgacg ccgcggccga ggcgctcttc gggacggtac cctgacgcgc gaaaccggct
     1021 tccatcgatc ggccttgcgc ctcctccgcg ccctggcctt cgccgtcctc gcggcggccg
     1081 ccctcgcggc ttgcgcctcc gctcccgcct cccccaaggc cggagacgcg gcttcgccct
     1141 ccgcgggcgc ggcaccggaa gccgcgccgc ccgccccggg agccgagggg caggccgcgc
     1201 cggccgccgc ggacgccccg tccgggcccg ccggctcgga agcgacagcc gcgcccgact
     1261 cgacttccgc ggccgccgtc ccctccgaga ccgcccctgc ctcggcgccc ggcgccggag
     1321 acgcgaaggg ccaggccgct gccaagcccg agctttcgga gtcacgcagg ctccccttca
     1381 ggctctcgct ccttctcctc aatctccccg atccggccca acccgctggc ctcgcggccc
     1441 cgaaggccgt ggcggccgcc gctccggccg ccgttcctgc accggcggcc aagccggctc
     1501 ccgtcgccct tcccgagccc gcgaagcccg caccggccaa gcccgccgcc gctccggccg
     1561 ccaaggccgc cgtccccgag aagactcccg ccgcccagcc caaggccgcc gccccggccc
     1621 cggccccggc ggcgaaaccg gcgccggcca aggccgcgcc gaccctgccc ccagcgccgc
     1681 catcgatcct ccccctgacc gatcccctcg cggagaaggc cgtggcgaag tccgcggaga
     1741 tcatgcgcaa ggtggaggcc gagcagggcc tcaggatcga gattcccttc gagggcacgg
     1801 gctggaccta cctgggcgag cgttccggca gcgagggcat cgtctacgag tcccggcgct
     1861 tcgagggctc cggcctcgtc ttcgccctgg gcgcgcagaa ggtgggtgac tacgtcctcc
     1921 gtttccagcg ccaggacctc ctccgcggca tcgtctacga cgagctcgtc ggcgtgacgg
     1981 tgaggccgag gccggccgtg cgggccgctc ccgcggcgga tcctacgccc gtctctccgg
     2041 cgtccagcgc cgccgcggca ggtgtcccgc cctcggcgag cccggccatc gcgacgccct
     2101 ctggcctccc ggccggcccc gcggccgccg ccacggcagg cggcgccgcg atcgccggga
     2161 cgggcggcgt gatgacgagc ggcgccctgg ccggcggggc cctcgcgacg ggcgccggaa
     2221 cgggcgccgc cgccacggca ggcggcgcaa tgcctggcgt caccgccggg gcgggcgccg
     2281 ccgcgactgg cggcgcgatg acgagcggcg cagccacggc cggccccggc gcggccgcga
     2341 acccggcctt gcccgccgcg gcggccgccc tcgcgcccga ttcccccgaa ggcctcctct
     2401 acctcgcgag gagcgagctc ggggcgggcc gcgtcagggg cgccctcgac gccctggacc
     2461 gcttcctctc cctctatccg gcggggatgg acgaggtcta cctcctctac ggcctggccc
     2521 atgagcagaa cggccccttc agggacatca agaaggccta cgccgagtac aagaagctcg
     2581 ccgccgacta tccgcagagc cccttctggg acagggccgc cgagcgcatc gcctacatcg
     2641 agcgccacta tctcgagatc cgctaacttt cccccctcga tttgacaagc ggctccccca
     2701 ggttctagta taataggagc tccggcgggc cgcggggcct cgacgccctc gccttcccgc
     2761 cgcccgacgc cggaagcagg agggaatccg tgaagagaat gatcctctgc gccgccctgc
     2821 tctgcgccgc ggcctcggcc gccttcgccc agaccgccaa cgccaaggct cccgcggccg
     2881 aggccgcccg ggtcgagggc cggcgctatg tcgtctattc cgagttggga ggggtcgcgg
     2941 ccgaggggct cgccgcccgc ctggacgcgc ttttcgagct ctacggcgcc tttttccgct
     3001 tcgatccggc ccttctccag ggcaagctcg tcgtgcggga attcgccggc aaggcggcct
     3061 tcgactccta cctggcgcag gtggccggcc agggcaagga ggatttcgtc tacctccact
     3121 accagagccc cgagcgccgc gagctcctgg tcttcgcgaa ggacgagccc gaccgctccg
     3181 cgagcctcgc ccaccaggcc ttcgtccagt acatcaaggc cttcgtccag aatcctccgc
     3241 tctggctccg cgacggcttc gccgtcttct tcgagacggc ggcctgggac gaggagaagg
     3301 ggagcctctc ctttagcgag aacctcgcct ggctcgagac ggcgaaggcc ctcgccgcca
     3361 agggctcctt cctccccctg gaaaggctgc tttcgatcac gcccgaggag tcccgctcct
     3421 cgctcgacgt ctactatccg gaggcctggg ccttcgtctc cttcctcgcg aacggcgccg
     3481 agggtggcta tgaccgtttc ctctgggaca cgatctccgg tctccgcaag gacgcgagcc
     3541 ttcaggagaa ccaggacgcc gcagccgccc gcctcgaggc ctggtacggc aagtccgact
     3601 cggaaaaggc cttcgcttcc tacctggagg gccgcctcac cttccccgag ctcgtgcagg
     3661 ccggcgtgaa gagctacggc gagaaggcct acgacgcggc ccgcgaggcc ttcgaggccg
     3721 cgagggagct cgacccagag tcctacgtgc cgccctacta cctgggcctc atcgcctacg
     3781 ccaagggcga cttcggcctg gcggacttcg agtacaagga cgccctgcgc ctgggctgcg
     3841 atcccgcgat cggcaactac gccctgggcc tcaacgccta cgcgcagaac cgcgcggagg
     3901 acgccaaggg cttcctgggc ctggccaagg aggccgcccc cgagcgctac ggcgccaagg
     3961 tggaggagct cctggcccgc ttccagaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnngc ttggggctgg cggcggcttg gcctgagcct aggcctgccc ttccaggcgg
     4141 cggttccgga accagagcgc gacgtccgtg gccaccatga ggccgttcag gacgtaaagc
     4201 caaaggacgc cgtcgtagcg gaccaggagc ttgttgagga tcccgcaggc gtagcccacg
     4261 aggatgacga tgagaaagaa gaggctcttt cccttggtgc tgcgggcctt ccaggatttc
     4321 gcgatggaaa ggggccaggc ggccccgaag cagagcaaca tgccgatctc gaagggactc
     4381 atggcgtaat tctctttctg gattgaggtt aaaaaaagag agggccgcgc ggggggagca
     4441 tttccggcgg ccgctctcgc gggcggctaa aggcgcccga cttgcctgga atcagtgctt
     4501 gaggacggcg aggatccgct ccaggacctt cttgcggtcg aggggcttca cgatatagct
     4561 cttcgcgccc aggaggaggg atttcttgac gacatcctcc ttgcccaggg cgctcaccat
     4621 cacgatggtc gcgttcttgt cgaactcgag gatcttctcg agggcggtca ctccgtccat
     4681 cttgggcatg gtgatgtcca tcgtcaccag gtccacgttc gggcagagct ccttgtactt
     4741 ctcgatgccg gccagtccgt cgccggccgt gccgaccacc tcgaagccct cagaggtcag
     4801 gatctggccg agctgcttcg cgatgaacat ggagtcgtcc acgatgagca cgcgatagga
     4861 cccgccgtcg gatttaatgc cgtcggggcg cttctcgttg atgttcggca tgtcggactt
     4921 ggcgatcata cgcttcaacg ctccttttct ctgaccgggc cgctttaagc ctagtggtct
     4981 actcgtaggt tatagtcgat gccggaggcc tttgcaaggc cgtcctggcc ggccgtcctg
     5041 gctggccagg acggccggcc aggagggacg ccacgtatgg cggccccggc aggcggccct
     5101 ggccggcggc cccctcaggc ggccccctca ggcggccccg gcttgcggcc ccggcttgcg
     5161 gccccggctt gcggccccgg cttgcggccg cctagatcag gtggcccatc ctgtcccgct
     5221 tggcggccag gtagccctcg tcgtgggggt tcggcggcat gaccagggga agccgctcgg
     5281 tcaccttcac cccctcggcc ctgagcttgt cgatcttgtc gggattgttc gtgaggaggg
     5341 cgatggagga gacgcccagg agggcgagca tcttcgccgc caccttgtag tcccgcccct
     5401 cgtcggggaa gccgaggtag cggttcgcct ccaccgtgtc caggccgagc tcctggaggc
     5461 ggtaggcctt gatcttgttg aggagaccga tgccccgccc ctcctgcctg aggtagagga
     5521 cggcgcccct ggggagcgct cctacctgcc ggagggccgc ctccagctgg tcgcggcagt
     5581 cgcagcgcag ggagccgagg acgtcgcccg tgtggcactc ggagtggacg cggacgggca
     5641 ggccctcggc gccggcgaca tcccccctga ccagggcggt gtgctccttg tcctcgcggg
     5701 cgtcgtagaa gccgtagagc tcgaaatcgc cgaagcgggt ggggaggtgg gcatgggcca
     5761 cgagctccac gcagaagtcc ccgtccgcgc agtaggcgcc cttgcccttg cagtagcgct
     5821 cctccagggg gaaatcgggc tcgccctcga gcttgcgcgc gagctcctcc ctccccaggg
     5881 ccttgtcgca gtccgcggtg aggcaggcgc gggtcagtcg ttccatggct tctcctttcc
     5941 ggccaggaac caatatactg gcccaggcgc ccgtcgggca aaaggcgcct agggctccgc
     6001 ggagcggagc gaaagggccc cttcgatgct cgagtccctc cttcccgtcc tccccctcgt
     6061 cctcctgctg ctcgcctggc tggccgaggc cttcctcctc ctcgccgccg ccatggccct
     6121 cctctcccac cgcctcatgg tcgggatcag ccggctggac gggaagacca tcgcggcccg
     6181 cctcctttcc aagggggagt tcggccccga ggccctggag ctcccctgga tcagggagag
     6241 gatagcctcg ccccacggct acgagctcgc cgtccacggc ctcaagggcg agggcgaccg
     6301 cctcgccatc ttccagcacg gcgtcggctg gaactggatg ggcgtcctca agtacatgga
     6361 gatcctccgc cgcgagggct ggagcgtcgc cgccctcgat tcccggggcc acggcgcctc
     6421 gggagggaag gatccctcct acggcttctt cgagaagggc gacctcctgg ccctggcgga
     6481 ctgggccctg gcccgcttcc cctcctccgg gggcttcgcc gccttcggcc tctccctggg
     6541 cgccgcctcg gtcctccagt acgcgccgct cgaccccagg ctggacgccg tggtcgccga
     6601 ctgtcccttc tcgagcgcgg cggaggagct cgaccaccgg ctcaagcgct ccttcgcccc
     6661 cttcttcctg aggcccctcg tcgtccggct catggacctc ctctgccgca ggctcgacgg
     6721 cttctccctc tacgcggcga gccccgagcg ggccatcctg gagacggaca agcccgtcct
     6781 cctcgtccac ggccgagagg acgactacgt gccctggcgc atgtcggcgc ggatggcgag
     6841 ggagcggcgg cgcctccttc ccgccgcccg gaccgagctc ctcctcgtgg agggcgcgac
     6901 ccatgcccgc tcccataagg cggcccccga ggcctacgag cgggcccttc tgggcttcct
     6961 ggacagcgcg gggcggccgt gctagtattc gcccaccatg agcatctaag gacgcggctc
     7021 ccttcttcag ggcggaccgc ctgtttcggc cccggcggag cgatacggct tgccgcccgg
     7081 gcctccttcc cacccaagaa gccgtttcgc ccgcggaggc cccctggccg ccgactgggc
     7141 gggccgccgg cgcggccccc agcgggggca aggcgcccga acgcgcgcga cgcgcgccat
     7201 tgacgaggac agattgaacg aactcaagga tacggctatc cgcccggaac gggccttcct
     7261 ggtcggggtc cagaccgaca agatctccaa ggcggaggcc gagagcctcc taaaggagct
     7321 ggggagcctc gcctccaccc tgggcgtcga gaaggcgggc tccctgctcg tgaagctccg
     7381 cgaccgcacc gccgccctcc tcctgggcac gggcaaggcg gacgaggtcg tcgccgccgc
     7441 caaggcggag ggggcggact ccatcctctt cgaccacgcc ctctcgcccg tccagcagcg
     7501 gaactgggag gcccgctccg acctcaaggt ctacgaccgc tccgagctca tcatcaagat
     7561 cttcgccagc cgcgccctca cgcgcgaggc cggcctccag gtggagctcg ccgagctcga
     7621 gttctcgctc ccgcgcctgg cccactccta cgaggccctc tcgcgccagc ggggcggccg
     7681 ctacggcacg aagggctcgg gcgagcagaa gatcgagctc gaccgccgcg agatcgagaa
     7741 gcggatccac gagatcaagg aggagctcaa gaccgcccgc aagagccgcg aggtgcagcg
     7801 gaagcggcgc gagcgccaga ccctcccccg ggccgcggtc gtcggctaca cgaacgcggg
     7861 caagtcgagc ctcctcaacg ccctcacgcg cgcgagcgtc cacgtggagg acaagctctt
     7921 cgcgaccctc gacccgacga ccaggcgcat cgccctggcc aagggcgggg ccctcctcct
     7981 caccgacacc gtcggcttcg tccgcaacct gccccacggc ctcgtggagg ccttcaaggc
     8041 caccctggag gaggcggccg cggccgacct cctggtccac gtggtcgacg cctccgatcc
     8101 cgaggccgag cggcacatgg cgacgaccga cgccgtcctg cgcgagatcg gcgcggggga
     8161 ggtgccgcgc gtcgtcgtcc tcaacaaggt cgacctcctt gaggaccgga gcctcctttc
     8221 ggggctcgcc gcgggccacg agggcgcgaa ggccgtctcc gtgaagaccg gggagggcat
     8281 ccccgagctc ctcgccgcca tagaggaggg cctcaccgcc cgggcgggcg aggtcgagct
     8341 caggatcccc gactcggact acgccttcgt ggcccttctc caccgcgagg cctccatcct
     8401 cgaggagtcg cgggacgacg aggggacgat cctccgctgc cgcctccccg accgcctcca
     8461 gtcccgcttc gagccctaca ggacggactc gggggcttcg ggggcggctt cgggcaggga
     8521 ggctgccggg gagtagggtc gcggccgagg gccgcgcgct ccgcgggaca agcgatcccg
     8581 gggccgggcg cggggatgcc gaaaaaacga aggccggccg gggcgtgaag cccggccggc
     8641 ctttttcgcc aggggcggcg ccccttggcg cggctggcgc ctggcgcgcc gtctgcggtc
     8701 gcgcgtctcc cggcgccggg aagtccggcg ccgcgaagtc cgcctagggc tgcggctctt
     8761 ccttcgcgta gaactggagg agctggacca tgaagtagat cttcttgtag acctcgacga
     8821 gccattcctt gatggggcgg ctggaggctc cctcgatctc cttccagttg atgaggatct
     8881 cgtggtggga gcggtcgtag tcctcgatga gggtcttgag gtagcggccc acgacgatga
     8941 ggttgacggc cgccttcgtg acgatgccca gggccttctc gttgatgtcc ttgagcatgg
     9001 tccggaggta cttcatctgc tccttgtcgc gctcgctctt cgcgagggcg ctccgcatcc
     9061 gcgtgccgat ctcgccctcg tcggccaggg actcgtcgaa ggccaggatc gcctccgagg
     9121 cctcgaggag gccgtggtag ccgtcggaga gctgctggga ctgcacgatg gtcgtccact
     9181 ggccgcggat gatgaggagg tcgacgagct ccctgatgtc cttcttgaag tagtccatga
     9241 ggtaggcctt gaggtagttg agggcgggcg cctgcgtgta gcccccgagc atcttcttcg
     9301 cgaagatgac gttggccttc tccgtgtagt tcttcatgcg gagcacgacg gaggtgccga
     9361 agacctgctt cgcgatctcg tcgatcttgg cgttgcgctt ttcctggacc aggcgctgga
     9421 ggagggtctc gagctgggcc ttgagcttct ggaggaaggg ctcgacgatg cgctcgttcg
     9481 ggtagcgggg ctgggcggcc caggccggcg tcttcttgat gtgcttcacg acgagctcga
     9541 ggacctggct cttgcgcacc tcggcgaccg cgggcaggag cttctgccag gcgtcgtgct
     9601 ggatgatctc cgcgcccttg tagtcgtgga gggcctggaa gatgcgcttc cagtcggcct
     9661 ccaggtccag ggggaggaag atctccagga agtcctcgat gtcgtccgcg acgtagtcgc
     9721 cgtttattgc ctcgaacttg ggcgcgcggc cgaagctgcg ctcgggcagg ctcgagtcgt
     9781 acttcttgag gaggaagtag tagtcgaagt tgacgaagtt gatgaaggag aggagggtgt
     9841 tgtaggcgga gtcgatctcc ctgatccggt ccccgtcgaa gacggcgtag aaggaggaga
     9901 ggtcgtcctt gacctcggcc tggagctcgc ggaggcctag gctcttcgcc cgctccagga
     9961 tgtgggcctc cgtcaggcgc tcggacagct ccttctggtc cttggtgagg aagctgtcga
    10021 tgacgaagga gcgcagggcg ctcgaggagg cggcgttgcc gaggaggacc tgggcggggg
    10081 ccgtgacctt gtagatctcg tagaagaagc gggccaggcc cggcagggcc tcctggccct
    10141 tgggccggta gaacttgaag cgcgaccgcg agaggtcctt ggcgatggcc ttgaggatct
    10201 tccgcttctc ggcgtcggga tcgcccatgc ccgcgaagaa ggagagcaga cgctggatga
    10261 ggttctcgcc gccccgggcc gcgtcgcggt cgggcgaagc cttcgagttc ataccgttca
    10321 ccttatatga tgggagccgc tccggtcaac tcgcggggcg cgaaagagga gctccccgga
    10381 ggcgaactcg agcggcctgt cggcgccggc cggccggagg aggagggccc ccgagggcgc
    10441 gaggccttcc aggacgcccg tgaccgtctc cccgccctcg ggggagcccg cgaggaaggc
    10501 cgcctcctcg ccccggcgcc agagccgctc ctcggcttcc tgtcgccagt ctccgtccgc
    10561 gaggacgaag gcgagctccc cgagcgcgag ctcgaggagc tcggggaggc cgaggccccc
    10621 tgggaggccg agctccaggg cgaggctcgt cgccttgcct tcgagctcgg gggggaagcc
    10681 gagctggttg acgttgaggc cgaagccgac cagggtcgcc tcggggccgg cctcgcagag
    10741 gacgcccgcg agtttcctcc cgccgaccag gacgtcgttg ggccacttga gcgcgggagg
    10801 ggaggagagg ctgccgcccc tcctgaccgc gaagacggcg gccgtccggc agagggccag
    10861 tcccacccgc aaggtgaagc ccggcagctt cgccgcctcg gggcccagga tgacggtcga
    10921 gaggaggttc tcgcccggag gcgaggccca tctgcgctcg ggaaggcggc cccgccccga
    10981 gctctgggcg tcggcgacga ccgtcgtccc gggcgggaag cccatcctta cgagcctgcg
    11041 ggcctcgtcc atcgtcgagg tggtctcgcg gaggcggaag acggccgctc cggggaaggc
    11101 gttgcggatc tcgagttgct ccattatcgc ctcactctag agcttttcgc cttccgtttc
    11161 aacaacgccg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggacagc
    11281 gcgagccgcg gggtcgggga tcgagaggag catcacatgt gcggaatcgt gggctacgcc
    11341 ggaccccggc gggccacccc catcctgctc gaggggctga agcgcctcga gtaccgcggc
    11401 tacgactcgg ccggcatcgc cgtcggccag gtcgaaggcg aggccccggg ctcccaggcg
    11461 ggccgcggag gccgcctggc cgtcgtcaag aagaccggca agatagccgc cctgcggagc
    11521 gaggtgcctc cggagctccc gggctcctgg ggcatcggcc acacccgctg ggccacccac
    11581 ggcggcgtca ccgacgcgaa cgcccacccc cacctggacg cctccggcaa gatcgccgtc
    11641 gtgcacaacg gcatcgtgga gaactaccgc tccctgcgct ccatgctgga gaaggagggc
    11701 gtccgcttcg ccagcgagac cgacagcgaa gtgatccccc atctcgtcgc caagtactac
    11761 tccggcgacc tcgaggccgc ggtgaaggag gccatcgcac ggctggaggg cacctacgcc
    11821 gtcgccgtca tccacgcgga cgagcccggc cggatcgtcg gcgcccgcaa cggcgggccc
    11881 ctcgtcgtgg gcgtgggcga cggcgagatg ttcctcgcga gcgacgtgaa cgcgatgatc
    11941 gcgcacacga aggaggtggt ctacctctcc gacggcgagc tcgtcgtcct cgatcccgag
    12001 ggcttccgca ccaccgaccg ccaggacagg ccagtcgcca agaaggtgga ccaggtctcc
    12061 tgggaggcgg gggccatcga gaagggcgac ttcgcgtgct acatggagaa ggagatcttc
    12121 gagcagcccg agtccatcga ccgcgccctg ggcgggcgcc tggaggccga ctcagcgacc
    12181 gccaagctgg gcggcctcaa cctggaccgg cgccagctcc gccaggtggg ccgggtccgc
    12241 gtcatcgccg cggggacgag ctggcacgcc ggcctcgtcg gcgcccagct cctcgagtcg
    12301 ctcgcgcgca tccccgccgg cgcggagctc gcgagcgagc tccgctaccg gaatcccgtc
    12361 gtcgagcggg acagcctctg gttcgccgtc tcccagtcgg gcgagacggc cgacaccctc
    12421 tacgcgatgc gggagatcca gaggaagggc ggcacggtcc tgggcgtctg caacgtcgtc
    12481 ggctccaccg tcgcccgcga gtcggacggc ggcatctacg tgcactcggg ccccgagatc
    12541 gccgtcgcct cgaccaaggc cttcacgagc cagctcgccg ccttctacct cttcaccctc
    12601 ctcatggcgc ggatgcggga cctctcccag gccgacggcg cgcgcttcgc cgaggccctc
    12661 gcgggcgtgc ccgcgaaggt cgaggagacc ctgcgatgcc gcggtgcggt ccaggcggcc
    12721 gcgaagaagt acgcccgcgc gacggacttc ctcttcctgg gccgcggcct catgtacccc
    12781 atcgccctgg agggagccct caagctcaag gagatctcct acgtccacgc cgagggctac
    12841 gcggcgggcg agatcaagca cggccccatc gccctcgtga gccccgagac gcccagcgtc
    12901 ttcctcgtgc cggacgacgc cttgcgcgag aagaccatct ccaacatgcg ggagatcaag
    12961 gcccgcggcg gccccgtgat cgcggtcggg gtcgaggggg acgatgaagt cgcggcgatc
    13021 gccgacgact tcatcgcgac cccgcgcatg gacccgcgct tctatccctt cacgatggtc
    13081 gtccccctcc agctcttcgc ctatttcacg gccctggagc tgggcaggga cgtcgaccag
    13141 ccgcgcaacc tggccaagtc ggtgacggtg gagtgatgcc gcgtcgggcg gggaggcggg
    13201 ccgcggcgcg gggagctctt cccgcgccgt tcatcctcct cgccttcctt gccgcctcgg
    13261 ggctcgcggt ccaggccgcg gcccaggcgg cggggggcgg cgccatcacg ggcggcgcgg
    13321 ctaggggcgg cgcggcggcg gccaggccgg agcttccgcc cgggcgcgcc ctccttctcc
    13381 gcgggcctga gccccgcaag ggcctgccct tcttctcgcc gaacgccatc cccgccctgg
    13441 tcggggagta cgaggtgtcc aagtcccttg tccgcgtgtg gagcagccgt tcgcccctca
    13501 gtttcgggaa gtcttgggaa tcgataccaa taaaacttcc cgcctctttg cgcgccttga
    13561 ggctcgcgcg ggatggcggc cccgtcatcg ccctggcggg gccagactac tctcttttct
    13621 tcgaattgcc cgccgacacg ccggccttca ggagcttcct cgccgccttc gagcgtcgct
    13681 tcgccttctt cttcgagggc gctccgaccg acgccgagct g
//
LOCUS       DAOOSJ010000038        13412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3085_length_13412_cov_6.222805, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000038 DAOOSJ010000000
VERSION     DAOOSJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13412
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_13412_cov_6.222805"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLG14_03290"
     CDS             <1..582
                     /locus_tag="PLG14_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOX48186.1"
                     /translation="PMMRSVCVFCGSSAGAGPAYVEAAEAMGRELAVLGVRLVYGGGN
                     VGTMGALARAALEAGGKVTGVIPRRLHEAVESVELTELIVAEDMHERKARMAEESDAF
                     IALPGGIGTFEELFEVWAWRQIGFHEKPVGLLDVGGFYRPLLAFLGGVVREGFLGKDS
                     LEDLVVDEEPGRLLDRLAAKGPPARTKLPERRR"
     gene            complement(676..>1365)
                     /locus_tag="PLG14_03295"
     CDS             complement(676..>1365)
                     /locus_tag="PLG14_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOX48187.1"
                     /translation="FALFPRSGGRNFDPETDEDWLRLGALAGRLHAVGRRRPAPARLR
                     LGPELAAARLEELLGLEAGEGGGGCIVHPEFAAEFEELARDALALAALRFEGSTVQRV
                     HGDLHRGNILDRPGTGLVLLDFDDMAMGPPAQDLWLLLPGRAADCGRELALLEEGYSE
                     FSSLDPWVFGLFEPLRLHRMLHFLVWRARQRGDHWFRREFPDWGGRAFWMKETEDLRD
                     ELEVLRDGARL"
     assembly_gap    1366..1465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1466..1784)
                     /locus_tag="PLG14_03300"
     CDS             complement(<1466..1784)
                     /locus_tag="PLG14_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOX48188.1"
                     /translation="MQRGSFDILSPGLAAEAVEEAYGLRLDGSVQVYPSYVNRVYGLK
                     DEEGRHFVAKFYRPGRWSREAVLDEHRFLADCLEADLPVAVPLPNDEGETLSELLLEE
                     EGGA"
     gene            complement(1804..3741)
                     /locus_tag="PLG14_03305"
     CDS             complement(1804..3741)
                     /locus_tag="PLG14_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX48189.1"
                     /translation="MNLISIDGASKTLGDSPLFEDLSLGVDEGDRIGLVGRNGAGKST
                     LLRMLSGSLEPDSGALARKRGLQVSVLPQRPSFRPGTSLRGFLLEGEAPEIALLRAYE
                     RLVAAGASSPASGQAAEAHERELERTHRALEESGALDLERRYASYCSELGLADLEAPM
                     ESLSGGMVKKAALARCLAPKSDLVLLDEPTNHLDVETIEWLERRLLASKTAFVLVTHD
                     RWFLDAVSSSILEIERGRVYRHPGNYSAYLERKAERWASMEKADSRRLANLKIELEWL
                     HRGARARAGKSRRRKELIRGMEGDYLERGASMDAFSSLESRLGRKIIEFKGVSKGYGA
                     KKVIEGLSYEFSKGDRVGVVGPNGAGKTTLLDLAAGRLEPDSGSVARGDTLKVAYFDQ
                     TGESLDRTKSVVDFVKEKAELIRRADGTVLTAEQLLERFLFPREFQKLALSRLSGGEF
                     RRLQLVRLLAESPNFLILDEPTNDLDIETIELLEDFLEGFQGCVLTVSHDRAFLDRVA
                     DFLVVLDGKGGSREFPGSYGEWRELEAEAAAEAAAQASRAAESARRREADEGRAGSED
                     GAAPAGGARKKLSFAERREFESILPELEALEEEKAALEALFSAAAADPAALKAANLRY
                     AELSPLIEAKTARWEELAERA"
     gene            complement(3930..6293)
                     /locus_tag="PLG14_03310"
     CDS             complement(3930..6293)
                     /locus_tag="PLG14_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48190.1"
                     /translation="MSPRPGLALGAAFLLGLASCLPLAAEADPLHVNLNDYPLYVKVG
                     FDAAALEPRPEGEPGWLAVPPTGELGRYARPIELDLPGVPKRPPFSLARYGQMEFSYA
                     IPFTMAPGSPLFASADSGGGSAGRQARPVPGIHLAALGDNWEIFLNGVRVRAELHLDA
                     KGKIASHRSVRDVYFPVDPDLFRPGRNLLLVRVIADPTFPPAGMHQAEPYFVGDYRSI
                     ERRNSELLPVVLCGLYLSIGLYHLFMFLVRRSDRHNLFYGLFSIDLAVYLFVRTHTAY
                     LLIPDSDVVFRVELAALSFILPFVGAFIETLNDNKVHRRTMAYGAYSALIALLEVTMP
                     VAFAHDVLRLWQYTGLAAALYYFSYGILGRFLRDGHRRWKRARDAGSSERLVSAYLRA
                     LGNTPIGNLLIGGVILFATAVFDIIDALFMQWDLVLTQYGFFVFTMGTALILANRFGF
                     LYERLQGLNLDLEDRIRLLTEASESLEASERKYKSLFHGSTEALALLDERLRFVEGNK
                     AAVELFELDRPGRKDRELGDALYVREREGRLQAERLRRAAEAQLRRAEAEELAVKLKT
                     PLGEPRPCRLRLERAGAGSGGLLLLRVLPEARDALAEAFVEGRERFEIDNTLSAAEEL
                     CRKACSRLSRYMPEEEAGFLEICLREIVVNAIEHGNLEISFDEKTESQREGRYFDFLL
                     ERRLEPAYRGRKVVLEYSVSAARATFRVTDEGKGFDHRALLAADGQPAPDLLEHGRGL
                     FMTLSAFDRVVYNEKGNQVTLVKYFPGAAAGAPGRGAAQAAEAASRN"
     gene            6427..7380
                     /locus_tag="PLG14_03315"
     CDS             6427..7380
                     /locus_tag="PLG14_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOX48191.1"
                     /translation="MRKFLDFLRSMRLALILIAYLAATGVLATLVPQGREPEFYAGAY
                     PKLVADLILSSGFSSFFRSWLFVLPSFLFFANLSACTMTRLLRELRKTGRRRHGPDIL
                     HVGLMLLVVGAVLTFSGRKEGFVRLAEGDAVELPDGRLLRLDRFEFLRYGDGRPKEWT
                     SQVSVLKDGKTEREAFPIRVNNPLKLGRLSIYQTSHSVERALVVRLAEAPSGQAVERS
                     LAQGEEFEAGGATLFFMAPDGGSGKALVRLKDAKGASSVLKLGPGEAAGPFLVASFGE
                     RDLTGLEAVVDPGYYVVLAALVLVGLGTFLAFIQKLKDQTE"
     gene            7377..8168
                     /gene="ccsA"
                     /locus_tag="PLG14_03320"
     CDS             7377..8168
                     /gene="ccsA"
                     /locus_tag="PLG14_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOX48192.1"
                     /translation="MNSLPTIAFALIALSAAVQAVFLLKGGGRKDRASRWLLLAAALL
                     LLATSVARSVEIGFPALTGTFESLVFYAALVCLVVFAYRAQKRLAYLPLVGFGATVFA
                     LVLLAVASSPLAPKAALAPIPALKSGWLVLHVSFTFVGEAFFVASFVSALAFLLSKDE
                     KRKLDYDRLTYTSIALGFPVFTAGALIFGAIWAERAWGSWWSWDPKETWALVTWLVYA
                     FYLHIRLTRRRKGALPAVAAVVGFLCTLFTFLGVNYLLPGLHSYG"
     gene            complement(8324..9889)
                     /locus_tag="PLG14_03325"
     CDS             complement(8324..9889)
                     /locus_tag="PLG14_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HOX48193.1"
                     /translation="MRKGSVEGSIRSRRGERAWIYGLGILTGVIGGLVAVAYRALAAW
                     LEGRRGGFLSRAEGGFALRAALWLAVVAAAGALTAFLVRKLPHIKGSGIPQVRATLMR
                     RISPDWRLELPAKFSGGVLALGAGLSLGREGPSIQMGALAGSGIARVAAKPELERYLV
                     TAGAAAGISAAFNAPLAGVLFCLEELHRNVSPVMLTCAMASAFAANAVSWILLGNAPV
                     FALGLGPSLPLRLYPSILLVGLCAGLLGKLFNTGLLRGQVLAARAVRSEALRVAAAFV
                     AAGSVALVLPALTGGGHLLVDEAAAGGLSVRVLALLLAGKFLFTIFCYSSGSPGGIFL
                     PMLVMGALLGGIFGQGLEAAGLAEGFGPNYVVLGMVAFFTAVVRAPITAAILISEMSG
                     SFAHFPALILVSVAASLTAGLLGSEPIYDSLLARLVPEPRAPKEEGTVVLHVPVLEGS
                     ALGSCEKARDCMPDGAVLVGVLRGEEERLPRADMAIHPGDVLEILAAEGEASEIKERL
                     LGLARPAKGSVEA"
     gene            10068..11729
                     /locus_tag="PLG14_03330"
     CDS             10068..11729
                     /locus_tag="PLG14_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48194.1"
                     /translation="MPKRLLIVGGVAAGATAAARARRLDENAEITIVEKGPYVSFANC
                     GLPYRLSGDIQKRSQLILQTPEGFEARYRVKVLLNTEALSIDRPGKKLRVRGPEGQAE
                     LPYDSLILAQGGTPFMPPIAGSDAAHVFRLWTIPDMDAIEAYIQKEKPESALVVGGGF
                     IGLEAAEAFLKRGLKVSVVELTDHLMPPADRDFGAMIGRAFEEAGAKVLVGRSVKSVD
                     AAAKTALLDDGTSVPAGLVLVSAGVRPNTELAKAAGLEIGPSGGLALDEGLRTADPSI
                     WGAGDMIEVSRIVDGKKVRVPLAGPANREGRVAATNALGGTAKFRGALGSSVFKAVDY
                     TFAMTGLSEKAARDSGFDAGAVVIHKGNHVSYMPGYEELSLKLVYDRKDGRILGAEAF
                     GKADVEKRIDVVAASILGGLTVHDLADLDLAYAPPYNSANDALNMAAFAAENELSGYS
                     PALSAAEAARLAASGEALILDVRTYGEHGRGHAEGSVHIPLDELRDRLGELSGERPIL
                     IVSRAGFEGHVALRQLVQHGFKNLRYISGGMTSLRLEPGYREAAE"
     gene            11764..12030
                     /locus_tag="PLG14_03335"
     CDS             11764..12030
                     /locus_tag="PLG14_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX48195.1"
                     /translation="MGSIKDKIKAGAAIIDVRTPDEFADGHYEGARNIPVNVLGNRLD
                     EVGPKDKPVVLYCASGARSAMAAKMLKASGFADVTNAGGLDDMP"
     gene            complement(12176..13270)
                     /locus_tag="PLG14_03340"
     CDS             complement(12176..13270)
                     /locus_tag="PLG14_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX48196.1"
                     /translation="MKTSPKVRGSAAPRFRKAKAAALAAVLAPALALAACSPRQEAGA
                     PKKLYIYNWTYYTPDSVIEKFEKEYGVDVVYDSFPSNEEMFAKLKAGGSDYDIVFPSG
                     DYVSIMAKEGMLAPLDKAKLPNFKNLDPAVLARCDFDPGNAYSVPYYMGAAGVAVNKT
                     KVPVFERSWSIFGRKDLAGKMTMLDDMREVLGDALAYSGKSVNSVSDADLELARRVVE
                     KEWLPNLLKFDAEAFAKGFAAGEFWVVQGYAESIYAEIDPAKRGDVEFFIPKEGGPMY
                     IDSMVVLKDSKNKDLAHAFIDFIHRPEMYAEFVDAFGFPATCNVPARALKKGDAWYTA
                     EALEPCELKADVGAGLEKYNAIWQEIRVGK"
BASE COUNT         1997 a         4580 c         4801 g         1934 t
ORIGIN      
        1 cccatgatgc ggagcgtgtg cgtcttttgc ggctcgagcg ccggcgcggg gccggcctac
       61 gtcgaggccg ccgaggcgat gggccgcgag ctggctgtcc tgggcgtgcg cctggtctac
      121 ggcggcggca acgtgggcac gatgggcgcc ctggccaggg cggccctgga ggccggcgga
      181 aaggtgacgg gcgtcatccc gaggcggctc cacgaggcgg tggagagcgt cgagctcacc
      241 gagctcatcg tggccgagga catgcacgag aggaaggcgc gcatggcgga ggagtcggac
      301 gccttcatcg ccctgcccgg cggcatcggg accttcgagg agctcttcga ggtctgggcc
      361 tggaggcaga tcggcttcca cgagaagccc gtcggcctcc ttgacgtggg cggcttctac
      421 cggcctctcc tggccttcct cggcggggtc gtcagggagg gcttcctcgg caaggactcc
      481 ctcgaggacc tcgtcgtcga cgaggagccg gggcggctcc tggaccggct cgcggccaag
      541 gggccgccgg cccggaccaa gctgcccgag aggcggcgct aggccgccgc cacccatccg
      601 gcgcggcggg ccgcgcaagg cgcggtccgg gcgctgcggc tggcgcccgt cacgcggcag
      661 gccgcggcgg gcctcctaga gcctcgcccc gtcgcgcagg acctcgagct cgtcgcggag
      721 gtcctcggtc tccttcatcc agaaggcgcg gccgccccag tcggggaact cgcgccggaa
      781 ccagtggtcg ccgcgctgcc tggccctcca gacgaggaag tggagcatgc ggtggagcct
      841 caggggctcg aagaggccga agacccaggg atcgaggctc gagaactcgg agtagccctc
      901 ctcgaggagg gcgagctcgc ggccgcagtc ggcggctcgg ccggggagga ggagccagag
      961 gtcctgggcg ggcggaccca tagccatgtc gtcgaagtcg aggaggacga ggcccgtccc
     1021 cggcctgtcc aggatgttgc cccggtggag gtcgccgtgg acgcgctgga cggtggagcc
     1081 ctcgaagcgg agggccgcga gcgcgagggc gtcgcgggcg agctcctcga actccgcggc
     1141 gaactcggga tggacgatgc atcctccccc gccctccccc gcctccagcc cgaggagctc
     1201 ctccaggcgc gcggccgcga gctcgggccc gagacggagc cgggccgggg ccggccgcct
     1261 ccgcccgacg gcgtggagcc gccccgcgag ggcgccgagc cggagccagt cctcgtcggt
     1321 ctcgggatcg aagttgcggc cgcccgagcg cgggaagagc gcgaannnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnccgcg ccgccctcct cttcgaggag gagctcggag
     1501 agggtctcgc cctcgtcgtt cggaaggggc acggccacgg gaaggtcggc ctcgaggcag
     1561 tccgcgagga agcggtgctc gtcgagcacg gcctcgcggc tccatcggcc tgggcggtag
     1621 aacttcgcga cgaagtgccg gccctcctcg tccttgaggc cgtagacgcg gttcacgtag
     1681 ctcgggtaga cctggacgct tccgtccagg cggaggccgt aggcttcctc gacggcctcg
     1741 gccgcgaggc cgggagacag gatgtcgaaa cttccccgct gcatggctcc tcctcgcctg
     1801 gcctcaggcc cgctcggcga gctcctccca tcgggcggtc ttcgcctcga tgagggggga
     1861 aagctcggcg tagcgcaggt tcgcggcctt gagggccgcg ggatcggcgg cggcggcgga
     1921 gaagagcgcc tcgagggcgg ccttctcctc ctccagggcc tcgagctcgg gaaggatgga
     1981 ctcgaactcg cgccgctcgg cgaagctgag cttcttcctc gcgccgcctg cgggggccgc
     2041 gccgtcctcg gacccggccc ggccctcgtc ggcctcgcgg cgcctcgcgg actccgccgc
     2101 gcgcgaggcc tgggcggcgg cctcggccgc tgcctccgcc tcgagctcgc gccactcgcc
     2161 gtagcttccc gggaactcgc ggctcccgcc cttgccgtcc aggacgacga ggaagtccgc
     2221 gacacggtcc aggaaggccc ggtcgtggga gacggtgagg acgcagccct ggaaaccctc
     2281 caggaagtcc tccaggagct cgatggtctc tatgtcgagg tcgttcgtcg gctcgtccag
     2341 gatgaggaag ttgggcgact ccgcgaggag gcggacgagc tggagccggc ggaactcgcc
     2401 gccggagagc cgcgagaggg cgagcttctg gaactcgcgg gggaagagga agcgctccag
     2461 gagctgctcc gcggtgagga cggtcccgtc cgcgcggcgg atgagctcgg ccttctcctt
     2521 cacgaagtcg acgacgctct tggtcctgtc cagggactcg cccgtctggt cgaagtaggc
     2581 gaccttgagc gtgtcgccgc gcgctacgct ccccgagtcg ggctccaggc ggccggccgc
     2641 caggtcgagg agggtcgtct tccccgcccc gttcggcccg acgacgccga cgcggtctcc
     2701 cttggagaac tcgtaggaga ggccctcgat caccttcttc gcgccgtagc ccttggagac
     2761 gcccttgaac tcgatgatct tccgcccgag ccggctctcc agggaggaga aggcgtccat
     2821 cgaggcgccc cgctcgaggt agtcgccctc catgccgcgg atgagctcct tccggcggcg
     2881 cgacttcccc gcgcgggcgc gggcgccgcg gtggagccac tcgagctcga tcttcaggtt
     2941 ggcgaggcgg cgggaatccg ccttctccat cgaggcccag cgctcggcct tgcgctcgag
     3001 ataggcggaa tagttgcccg gatggcggta gacgcggccc cgctcgatct cgaggatcga
     3061 ggaggagacg gcgtccagga accagcggtc gtgggtgacg aggacgaagg ccgtcttgga
     3121 ggcgaggagg cggcgctcca gccactcgat cgtctccacg tccaggtggt tcgtcggctc
     3181 gtcgaggagg acaaggtcgc ttttcggcgc gaggcagcgc gcgagggccg ccttcttcac
     3241 catgccgccg gagaggctct ccatcggcgc ctcgaggtcg gcgaggccga gctcggagca
     3301 gtaggaggcg tatctccgct ccaggtccag ggctcccgac tcctccaggg cgcggtgcgt
     3361 gcgctcgagc tcccgctcgt gggcctccgc ggcctggccc gaggccggcg acgaggctcc
     3421 ggcggcgacg aggcgctcgt aggcgcgcag gagggcgatc tcgggcgcct cgccctcgag
     3481 gaggaagccg cggagactcg tccccggccg gaaggagggc cgctggggga ggacggaaac
     3541 ctggaggccg cgcttgcggg ccagggcgcc cgagtcgggc tccagggagc ctgagagcat
     3601 gcggagcagg gtggacttgc cggcgccgtt ccggccgacg aggccgatcc ggtcgccttc
     3661 gtcgacgccc agggataggt cttcgaaaag cggggagtcg cccagggtct tcgacgcgcc
     3721 gtcgatggag atgaggttca cgggtggact atagccgatt ccgcgggcct tagcctacgg
     3781 ccttcggttc cgcgcggctg ggcggagggc cgtggccgcg cgtttgccgc accccccggc
     3841 ggccgtggcc gcgctggggg acgaagcggc gccggaccga gccgcggcgg ccgtgtccgc
     3901 cgctccggga gccaacgccc aggcggccct cagttcctgg aggccgcctc cgcggcttgc
     3961 gcggcgccgc ggccaggcgc tcccgcggcg gcgcccggga agtacttcac cagggtcacc
     4021 tggttgccct tctcgttgta gacgacgcgg tcgaaggcgc tcagggtcat gaagaggccg
     4081 cggccgtgct ccaggaggtc gggcgccggc tggccgtcgg cggcgaggag ggcgcggtgg
     4141 tcgaagccct tcccctcgtc ggtgacgcgg aaggtggcgc gcgcggcaga gacggagtac
     4201 tcgaggacga ccttccggcc gcggtaggcc ggctcgagcc tgcgctcgag gaggaagtcg
     4261 aagtagcgcc cctcgcgctg gctctcggtc ttctcgtcga agctgatctc caggttgccg
     4321 tgctcgatcg cgttgacgac gatctcgcgg aggcagatct cgaggaagcc cgcctcctcc
     4381 tccggcatgt agcgggacag gcgcgagcag gccttgcggc agagctcctc cgccgcgctc
     4441 agggtgttgt cgatctcgaa gcgctcgcgg ccctccacga aggcctccgc gagggcgtcc
     4501 ctggcctccg ggaggacgcg gaggaggagg aggccgcccg agcccgcgcc ggcgcgctcg
     4561 aggcggaggc ggcagggcct cggctctccc aggggggtct tcagcttgac ggcgagctcc
     4621 tccgcctcgg cccgccggag ctgggcctcg gccgcgcggc ggaggcgctc cgcctgcagc
     4681 cggccctcgc gctcgcggac gtagagggcg tccccgagct cgcggtcctt cctgccgggc
     4741 cggtcgagct cgaagagctc gacggccgcc ttgttgccct cgacgaagcg cagcctctcg
     4801 tccaggaggg cgagggcctc cgtcgagccg tggaagaggc tcttgtactt gcgctcgctc
     4861 gcctccaggc tctcgctcgc ctccgtgagg agccggatgc ggtcctccag gtccaggttg
     4921 aggccctgga ggcgctcgta gaggaaaccg aagcggttcg cgaggatgag ggccgtgccc
     4981 atcgtgaaga cgaagaagcc gtactgcgtg aggacgaggt cccactgcat gaagagggcg
     5041 tcgatgatgt cgaagaccgc ggtcgcgaag aggatgacgc cgcctatgag gaggttgcct
     5101 atcggcgtgt tgccgagggc gcggaggtag gcggatacga ggcgctccga gctccccgcg
     5161 tccctggcgc gcttccagcg ccggtggccg tcgcggagga agcggcccag gatgccgtag
     5221 ctgaaatagt agagcgccgc cgcgaggccc gtgtactgcc agagcctcaa gacgtcgtgg
     5281 gcgaaggcga cgggcatcgt cacctcgagg agggcgatga gggcggagta ggcgccgtag
     5341 gccatcgtgc gcctgtggac cttgttgtcg ttgagggtct cgatgaaggc gccgacgaag
     5401 gggaggatga agctcagggc ggcgagctcg acgcggaaga cgacgtcgga gtcggggatg
     5461 aggaggtagg ccgtgtgggt gcggacgaag aggtagaccg cgaggtcgat ggagaagagg
     5521 ccgtagaaaa ggttgtgccg gtccgagcgc ctgaccagga acatgaagag gtggtagagg
     5581 ccgatggaga ggtagaggcc gcagaggacg acgggcagga gctcggagtt acgccgctcg
     5641 atggagcggt agtctccgac gaaatagggc tcggcctggt gcatgccggc gggggggaag
     5701 gtgggatcgg cgatgacccg cacgaggagg aggttccgcc ccggccggaa gaggtcggga
     5761 tcgacgggaa agtagacgtc gcggacgctg cggtggctcg caatcttgcc cttcgcgtcg
     5821 agatggagct ccgcgcggac gcgtacgccg ttgaggaata tctcccagtt gtcgcccagg
     5881 gcggccaggt ggatgccggg cacgggccgc gcctggcggc cggcgctccc gccgcctgag
     5941 tccgccgagg cgaagagggg cgagcccggc gccatcgtga aggggatggc gtagctgaac
     6001 tccatctgcc cgtagcgggc cagggagaag ggaggccgct tgggcacccc ggggaggtcg
     6061 agctcgatgg gccgggcata gcggcccagc tctcccgtcg gcgggacggc gagccagccg
     6121 ggttcgccct cgggccgggg ctccagggca gccgcgtcga agccgacctt cacgtagagc
     6181 ggatagtcgt tcaggttgac gtggagggga tcggcctcgg ccgcgagcgg aaggcaggag
     6241 gcgaggccga ggagaaaggc cgcccctagg gcgaggccgg gtcgagggct cataggctgg
     6301 cattatagtg cctcctcgcg gaatcgccac ggggattcgc gctttttctc cccgatatgc
     6361 cttgaattga caaggccccg cgcggcgcct atcctgggac atcacttcca cctaggcgga
     6421 gcggctttgc gcaaattcct ggacttcctg cgatcgatga ggctcgccct catcctcatc
     6481 gcctatctcg ccgcgacggg cgtcctggcc accctcgtgc cccagggacg cgagccggag
     6541 ttctacgcgg gcgcctatcc gaagctcgtc gccgacctca tcctctcttc cggcttctcg
     6601 agcttcttcc gctcctggct cttcgtcctg ccctccttcc tcttcttcgc gaacctctcg
     6661 gcctgcacga tgacgaggct cctccgcgag ctcaggaaga cggggcgccg ccggcacggc
     6721 cccgacatcc tccatgtcgg cctcatgctc ctggtcgtcg gggccgtcct caccttctcc
     6781 ggacggaagg agggcttcgt gcgcctggcc gagggcgacg cggtggagct gcccgacggc
     6841 cgcctcctca ggctggaccg cttcgaattc ctgcgctacg gcgacgggcg gccaaaagag
     6901 tggacgagcc aggtctccgt gctcaaggac ggaaagaccg agcgggaggc ctttcccatc
     6961 agggtcaaca accccctgaa gctcggccgg ctctccatct accagacctc gcacagcgtg
     7021 gagcgcgccc tcgtcgtgcg gctcgcggag gcgccctcgg gccaggccgt cgagcggagc
     7081 ctcgcccaag gcgaggagtt cgaggcgggc ggcgccacgc tcttcttcat ggctcccgac
     7141 ggcgggagcg gcaaggccct cgtccggctc aaggacgcga aaggcgcttc ctcggtgctg
     7201 aagctcgggc caggcgaagc cgcgggcccc ttcctcgtcg cctccttcgg cgagcgcgac
     7261 ctgaccggcc tcgaggccgt cgtcgatccg ggatactacg tcgtcctcgc ggccctcgtc
     7321 ctcgtcggcc tcgggacctt tctcgccttc atccagaagc tcaaggacca aacggaatga
     7381 acagcctgcc cacgatcgcc ttcgccctga tcgccctttc ggcggccgtc caggccgtct
     7441 tcctcctcaa agggggcgga aggaaggaca gagcctcgcg ctggctcctc ctcgccgccg
     7501 ccctcctcct cctcgcgacc agcgtcgccc gaagcgtgga gatcggcttc ccggccctca
     7561 cgggcacctt cgagtccctc gtcttctacg ccgccctggt ctgcctggtc gtcttcgcct
     7621 atcgcgccca gaagcggctg gcctacctgc ccctcgtggg cttcggcgcc acggtgttcg
     7681 ccctcgtcct cctcgcggtg gcgagctcgc ctctcgcccc caaggcggca ctggcgccca
     7741 tccccgccct caagtcgggc tggctcgtcc tccacgtctc cttcaccttc gtcggcgagg
     7801 ccttcttcgt ggcctccttc gtctccgccc tcgccttcct cctctccaag gacgagaaga
     7861 ggaagctcga ctacgaccgc ctgacctaca cctcgatcgc cctcggcttc ccggtcttca
     7921 cggccggagc cctcatcttc ggcgcgatct gggccgagcg cgcctggggc tcctggtggt
     7981 cctgggaccc gaaggagacc tgggccctcg tcacctggct cgtctacgcc ttctacctcc
     8041 acatccgcct cacgaggcgg cggaagggcg cccttcccgc cgtcgcggcc gtggtcggct
     8101 tcctctgcac cctcttcacc ttcctgggcg tgaactacct cctgccgggc ctgcacagct
     8161 acggctgagg aggcgccgcg gttccggcct gggacccggc gccacgggcg cccggcgcca
     8221 cgggcgcccg gcgccacggg cgcccggcgc tgccggctct cggctttgct gacgccaggc
     8281 gcttcgcgct cgccgtcggc gcgaaggcgg cggccccgcg atcctaggcc tccaccgagc
     8341 ccttcgccgg ccgcgcgagg ccgagtagcc gctccttgat ctcggaggcc tccccctccg
     8401 ccgcgaggat ctcgagcacg tccccgggat ggatggccat gtccgcgcgc ggaagccttt
     8461 cctcctctcc cctgagcacg ccgacgagca cagccccgtc gggcatacag tcccgggcct
     8521 tctcgcagct ccccagggcc gatccctcga ggacgggcac gtggaggacc accgtgccct
     8581 cttccttggg agcgcggggc tcgggcacga gcctcgcgag gagggagtcg tagatcggct
     8641 ccgagcccag gaggccggcc gtcagggagg ccgccacgga gacgaggatg agggcgggga
     8701 aatgcgcgaa gctgcccgac atctcgctga tgaggatggc ggcggtgatg ggcgcccgga
     8761 ccacggcggt gaagaaggcg accatcccga ggacgacgta gttggggccg aagccttcgg
     8821 cgaggcccgc ggcctccagg ccctggccga agatgcctcc gaggagggcg cccatcacga
     8881 gcatgggaag gaagatgccg cccggcgagc ccgaagagta gcagaagatc gtgaagagga
     8941 atttccccgc gaggaggagg gccaggacgc ggaccgaaag gccgccggcg gcggcctcgt
     9001 cgacgaggag atggccgccg cccgtgagcg ccggcaggac gagggcgacg gagccggccg
     9061 cgacgaaggc ggccgcgacg cggagcgcct cgcttcggac ggcccgagcc gcgaggacct
     9121 ggccccgcag caggcccgtg ttgaagagct ttcccaggag gccggcgcag agccccacga
     9181 ggaggatgct cggatagagg cgcaagggca ggctcggccc caggccaagg gcgaagacag
     9241 gcgcgttgcc caggaggatc caggataccg cgttggccgc gaaggccgag gccatggcgc
     9301 aggtgagcat cacgggcgag acgttgcggt gcagctcctc gaggcagaag agcacgccgg
     9361 ccagcggcgc gttgaaggcg gccgagatgc cggccgcggc gccggccgtg acgaggtagc
     9421 gctcgagctc gggcttggcg gcgacacggg ctataccgga gcccgcaagg gctcccatct
     9481 ggatggaggg accctcccgc cccagggaga gccccgcgcc gagggcgagg acgcccccgg
     9541 agaatttcgc cggcagctca agcctccaat ccggggagat gcggcgcatc agcgtcgccc
     9601 ggacctgcgg gatgcccgat cccttgatgt ggggaagctt ccggacgagg aaggccgtga
     9661 gggcgccggc cgcagcgacg acggcgagcc acagggccgc gcggagggcg aagccgcctt
     9721 ccgcgcgcga aaggaagccg ccgcgacggc cctcgagcca ggcggcgagc gcgcggtacg
     9781 ctacggcgac gaggccgcca atgacgccgg tgaggatgcc gagtccgtag atccaggccc
     9841 gttcgccccg gcgggaacgg atggatcctt cgacgctgcc ttttctcatg atgccgacta
     9901 tactaatgaa gacgggcagg gaggctcaag ctcggacgtc ttcttttgac gcggccttct
     9961 cctgcgtctc gccatggagg cccgcttttt cggatatggg gctctctccg ccttcttgac
    10021 aatatatgtt tttcaatata tattaaccct gcaacaagga ggacgaaatg cccaagagac
    10081 ttctgatcgt cggcggagtg gccgccggag ccacggccgc cgcccgtgcc aggcgcctgg
    10141 acgagaacgc cgagatcacg atcgtcgaga aggggcccta cgtctccttc gcgaactgcg
    10201 gcctccccta ccggctctca ggcgacatcc agaagcggag ccagctcatc ctccagaccc
    10261 ccgagggctt cgaggcccgc taccgggtca aggtcctcct aaacaccgag gccctctcca
    10321 tcgaccggcc cggcaagaag ctccgcgtcc gcggccccga gggccaggcc gagcttccct
    10381 acgactccct catcctcgcc cagggcggga cgcccttcat gccgcccatc gccggctccg
    10441 acgccgccca cgtcttcagg ctctggacca tccccgacat ggacgcgatc gaagcctaca
    10501 tccagaagga gaagcccgag agcgccctgg tcgtaggcgg cggattcata ggcctcgagg
    10561 cggcggaggc cttcctcaag cgcggcctca aggtgagcgt cgtcgagctc accgaccacc
    10621 tcatgccccc cgcggaccgc gacttcggcg ccatgatagg acgggccttc gaggaggccg
    10681 gggcgaaggt cttggtcggc cgctcggtga agtccgtcga cgcggcggcc aagaccgccc
    10741 tcctcgacga cgggacctcg gtgcccgccg gcctcgtcct cgtctccgcg ggcgtgcggc
    10801 ccaacacgga gctcgcgaag gccgccggcc tggagatagg cccctcgggc ggcctcgccc
    10861 tggacgaggg cctgcgcacg gccgacccct ccatctgggg cgcgggcgac atgatcgagg
    10921 tgagccgcat agtcgacgga aagaaggtcc gcgtccccct cgccggcccg gcgaaccgcg
    10981 agggccgcgt cgccgcgacc aacgcgctgg gcgggacggc gaagttccgc ggggccctgg
    11041 gctcgagcgt cttcaaggcc gtcgactaca ccttcgcgat gacgggcctc tcggagaagg
    11101 ccgcgcggga ctcgggcttc gacgcgggag ccgtcgtgat ccacaagggg aaccatgtct
    11161 cctacatgcc cggctacgag gagctctccc tcaagctcgt ctacgacagg aaggacggga
    11221 ggatcctggg agccgaggcc ttcggcaagg ccgacgtgga gaaacggatc gacgtcgtcg
    11281 cggcgtccat cctgggcggg ctcaccgtcc acgacctggc cgacctggac ctggcctacg
    11341 ccccgcccta caactccgcg aacgacgccc tcaacatggc cgccttcgcc gcggagaacg
    11401 agctctccgg ctactcgccc gccctgtccg ccgccgaggc cgcccgcctc gccgcctcgg
    11461 gggaggccct catcctggac gtcaggacct acggggagca cggccgcggc cacgcggagg
    11521 gctcggtcca catccccctg gacgagctca gggaccgcct gggcgagctg tccggggaaa
    11581 ggccgatcct catcgtctcc agggcaggct tcgagggcca cgtcgccctc cgccagcttg
    11641 tccagcacgg attcaagaac ctcaggtaca tctcgggcgg catgacgagc ctccggctcg
    11701 agcccggcta ccgcgaagcc gccgagtagc ggcggcggaa cacccacacc caaggagaca
    11761 gacatgggca gcatcaagga caagatcaag gccggggcgg cgatcatcga cgtgcggact
    11821 cccgacgagt tcgccgacgg ccactacgaa ggagcccgga acatccccgt gaacgtcctc
    11881 ggcaaccgcc tggacgaggt ggggcccaag gacaagcccg tcgtcctcta ctgcgcctcg
    11941 ggcgctcgct cggccatggc cgcgaagatg ctcaaggcct cgggcttcgc cgacgtcacg
    12001 aacgccggcg gcttggacga catgccgtga gcggcggccg ctaggccggc cgcgcgcgac
    12061 ggagccgcgc ccgccgcgga accgagtccc gaaacgcggc gcggcgaaac gcgatgagct
    12121 ggaaaagaaa gggccgcccg gcgagggcgg cccctttcgc ttcatcgggg cgcggtcact
    12181 tcccgacgcg gatctcctgc cagatcgcgt tgtacttctc caggccggcg cccacgtcgg
    12241 ccttgagctc gcagggctcc agggcctccg ccgtgtacca ggcgtcgccc ttcttgagcg
    12301 cccgcgcggg gacgttgcag gtcgcgggga agccgaaggc gtcgacgaac tcggcgtaca
    12361 tctccggccg gtggatgaag tcgatgaagg cgtgggcgag gtccttgttc ttcgagtcct
    12421 tgaggacgac catggagtcg atgtacatgg ggccgccctc cttcgggatg aagaactcga
    12481 cgtcgccccg cttcgcggga tcgatctccg cgtagatcga ctccgcgtag ccctggacga
    12541 cccagaactc gccggcggcg aagcccttcg cgaaggcctc cgcgtcgaac ttgaggaggt
    12601 tgggcagcca ctccttctcg acgacgcggc gcgcgagctc caggtcggcg tccgacaccg
    12661 agttgacgga cttgcccgag taggccaggg cgtcgcccag cacctcgcgc atgtcgtcga
    12721 gcatcgtcat cttgcccgcc aggtccttcc tcccgaagat cgaccagctt ctctcgaaca
    12781 cgggcacctt cgtcttgttg acggcgacgc cggccgcgcc catgtagtag gggacggagt
    12841 aggcgttgcc cggatcgaag tcgcaccgcg cgaggacggc cgggtccagg ttcttgaagt
    12901 tgggcagctt ggccttgtcc aggggggcga gcatgccctc cttggccatg atggagacgt
    12961 agtcgcccga ggggaagacg atgtcgtagt cggagccgcc ggccttgagc ttcgcgaaca
    13021 tctcctcgtt ggaggggaag gagtcgtaga cgacgtcgac tccgtactcc ttctcgaact
    13081 tctcgatcac ggaatccggc gtgtagtagg tccagttgta gatgtagagc ttcttcggcg
    13141 ctccggcctc ctgccggggg gagcaggccg cgagggcgag ggccggcgcg aggacggcgg
    13201 cgagggccgc ggccttggcc ttgcggaagc ggggagccgc ggagccgcgg accttggggg
    13261 aagtcttcat gacgacctcc ttgacgtgaa ggatgtggcg gccgttccgg cgcgccggcg
    13321 gccccagggg ccgccttccg gcgaagcgac cctcgcgccc ctaggaatgg gcggcgaagg
    13381 tcttgaggaa attgcgcaag ggatatacga gg
//
LOCUS       DAOOSJ010000039        13262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3124_length_13262_cov_6.084122, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000039 DAOOSJ010000000
VERSION     DAOOSJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3124_length_13262_cov_6.084122"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLG14_03345"
     CDS             <1..1213
                     /locus_tag="PLG14_03345"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOX48197.1"
                     /translation="PRMKPPRAAAKAWPCLALAFALSLSIPAALSSCAGRGAPGQPAA
                     GGAAGPSADGQGGSDEALLQARAKELALRLPVVDTHIDYPEVRKPAEDLLLGTSGNFD
                     HPRAVAGGLDAAFLSIFVPAEDEEAGKAFAKAEERLSLIEADLAAHPEALALAASPEE
                     VARNAAAGRLSLALGLENAAPIEGRLERVAAFRKRGVSYVTLCHLKNNHVCDSANDES
                     GPRWGGLSPFGRELVAELNARGVMVDLSHVSEAAFWEALALTKAPPIASHSGLRSFTP
                     GFERNLSDEMLAALAAKGGVVQIPFGSSFLSEKYRQTMSYMALATVRDVALVVDRAVQ
                     VAGVDHVGIGSDFDGVGFSLPRELRDVSMYPNLVRELLRLGYSEEAIGKILGGNILRV
                     WGEAIGAGRLP"
     gene            1257..1697
                     /locus_tag="PLG14_03350"
     CDS             1257..1697
                     /locus_tag="PLG14_03350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX48198.1"
                     /translation="MRSPLVLVILLALSGSFSFFLSGYGLSRKRVSGALEFAGFMAAS
                     ALYAVGYAVELTQGELSGMLAVLMPDTGLEGADRAAERFRLALSSEPVAWKGEPIAVT
                     TSVGYCAVVPEEGERLADYLGRADLALYRAKDSGRNRSVAWTPA"
     gene            1987..2445
                     /locus_tag="PLG14_03355"
     CDS             1987..2445
                     /locus_tag="PLG14_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX48199.1"
                     /translation="MDLAKWGAPATRDLWEAFDGLRGDMDRALDFFSLPDAAGLLDRT
                     TAPAVDIVETEEEYLVVADLPGVDKRDLEVSVTGSLLAIKGVKKAEGNSGKRKVFRKE
                     TWVGSFSRTIDLPAEIDPGKIKAELKDGVLALRIAKREEAKTKLIAVNVQ"
     gene            2457..2807
                     /locus_tag="PLG14_03360"
     CDS             2457..2807
                     /locus_tag="PLG14_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX48200.1"
                     /translation="MDQRYIDNARGVAVPACSITEDEGLVTVKLEMPGVPKEELEVRI
                     EGNSLEVSGTRRRAEERGKYLLRERRADSFRKRFTLDETIDREKVAAELADGILVLKL
                     HIREAAKPRKIEIS"
     gene            2872..3108
                     /locus_tag="PLG14_03365"
     CDS             2872..3108
                     /locus_tag="PLG14_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HOX48201.1"
                     /translation="METSPLEDPSYLIRLATARMPFGKYAGRLLIDLPEGYVVWFSRQ
                     GYPDGEIGELLASLYVIKENGLESLLRPLVGGPR"
     gene            complement(3199..4131)
                     /locus_tag="PLG14_03370"
     CDS             complement(3199..4131)
                     /locus_tag="PLG14_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOX48202.1"
                     /translation="MRRRVLGSLSASLLLAFSLASCATTKVPELKYPVALAVGADQRI
                     YFADRDLGSLFRMDDIAGKGLVGYGTKGSGGPGRFLSLEGRVAFDSLGRIHIVDCKNA
                     KVIRFDDMRGGNWTELSTAAYGLPYPAGVAVDREGRVYVTDIAVHAVARFDSMGGEGF
                     VRLGERGKGEGQFSLPFAVAVDGDNRVYVADEMNGRIVRFDDMSGAGWTSLDGRGVPS
                     DRGFVAPADIKLLPDGTILVADADGGRLFSFASMDGSGRRVFGWDGVGGRTMYPLGVG
                     VDSSGRLYFTDNQAKQVIRVDGFDGAGLVGFPAP"
     gene            4394..5758
                     /locus_tag="PLG14_03375"
     CDS             4394..5758
                     /locus_tag="PLG14_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48203.1"
                     /translation="MALGFACVLLASPLAAVFAEGAPSGEAYAEAKAKALSWLEARMV
                     PNDTVPEPEPDRMRLLLSYEIPPSEPAYRYLSGRSMVYDDALAAVAFTMAGDYRNAAY
                     VLNALSRLSRADGGLWFGYNTHNSWPSEDDADGALDRTGSGAWAGYAAVYYIQKRERG
                     EPGFLARDREARSYLEMAKAMADCLLSRVVKDGKDPRAGLATGGGGGYSLGYEAKEVV
                     EVFAPGAVDWASAEHNIDLYFLLKELGALTGESRYLEAAETAKAALLGLWSARDGQYY
                     QGRKPDRIDRVLALDCASWGAMFSQAAGREDYARACLKAVEARYRSSDALPSSVGGRV
                     EGYKPYADRLLFEGAPEEVAEHYFGGAKAPTWRETEGVWGEGSLGVALAYLKQGKAGK
                     AKAILDEMLKLQTPGGGLPYFTREIPHEFVSYPSVASTAWLAIAISALEDPEGSSGFM
                     GNWR"
     gene            5755..7605
                     /locus_tag="PLG14_03380"
     CDS             5755..7605
                     /locus_tag="PLG14_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48204.1"
                     /translation="MRRAGRFPAMRLALALSLALAAAATASAQVSAGQAGAGQAGAGL
                     SGARLGRLSRVAVLDLDARNGEAGTEALAVKRCLDILGIACLVTADPAEATREAMVCA
                     AGGLWNGTLTSREFYGLAAWVESGGILLSSGGLGSKYFPLFGIGQPVDSRKRFRVRFE
                     APGTDPSLAYLDRPEERETRLGDEKLFKEVVWTRSFPVKGARSLARTEDGLSLFSVAD
                     YGKGRAYALGLSFTDAVLLPQVGGDYEAQRSWINGFEPGADVFILLLKAIFEDSFGPF
                     VYLSAVPEGRKTALLLSHDVDAQSSFAHSVDFAAMERRRGARSTFFVTTKYFADETDA
                     AYWDPARAALIRKAKELGGDVGSHSVAHSRRFGDFPSGDAAVRKADYDPVRSPTVLGE
                     LLVSKELLDGGLPGQRTRAFRAGELRIPRGLGSALELAGYRYDSSYSANDTMTNFAYR
                     MMRDRSPGSGESGIVEIPVTFDDSQGLLTASNWRSVVAAWLDVVWANAANEAISVLLI
                     HPTVAGYKLEAEELLLESLEGKGIWIGDVSSYGAFWNERAALGYEARLEGDRLVVRLG
                     GESLPPGQCLVAGRTPLVKSVEVRGSGGAVLDCETTERDGKLFIRARGAK"
     gene            7714..>8156
                     /locus_tag="PLG14_03385"
     CDS             7714..>8156
                     /locus_tag="PLG14_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48205.1"
                     /translation="MYGIDFGTSNTVVTARAGGPAAVAPAEGAAADGTARPGGPGGAA
                     SAAAARVLDLGEGGVLPSLLYFERDRRPSIGAEAMADYAAALSGSRGQANLLSRFRFF
                     QAIKLALKDPYFEGTRIFGDYVRVEALAALFLRETKRRADEALAA"
     assembly_gap    8157..8256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8257..9112
                     /locus_tag="PLG14_03390"
     CDS             <8257..9112
                     /locus_tag="PLG14_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOX48206.1"
                     /translation="DARLEGRFREAALLAGFDSVDFVYEPVAASVDALDGGRGRGTVL
                     VFDFGGGTLDISVARGGERGVEILSSAGMDLGGYLLNEDLARARIVGHFGAGGKWRTM
                     TGRWLEMPAWITNQVASFYALPLADLQRTRTTVKELVYDARREDKDKLRGLVEFLDRN
                     LAFELFARIDGAKIELSGAEESSIAYAVPPYIEFEERLARADFEALVAPRVEAARLLV
                     LEALAKAGVEAGRVDRVVRVGGSSRVPAFVRMLEGLFSGRVEEGAVFTSIASGLIAAR
                     DRGLVAGA"
     gene            9343..10272
                     /locus_tag="PLG14_03395"
     CDS             9343..10272
                     /locus_tag="PLG14_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX48207.1"
                     /translation="MRRLTSLGLLLAATVLVLGVSCAKAPEKAPIVVASMIDSEGALL
                     GKMIVQVLEANGIPVLDKTEFGTPDVLRKALEAKEVHLVVDYTGSGQYYHEGQDPAVW
                     SDAAKGYEATRRLDAAKGIVWLKPSPANNTESLAVKRSFAERTGVRDLPALAAYARRG
                     GEVKLICAQSYADNPLGLIGLEKAYGFEFRKDQLILLSSGNTAEMLKALSEGTNGVNV
                     SLVYGTDGALDKMELLVLADPKSVPPVYLPAPVILEEKLAESPKIEELLAPVFASLDL
                     EILQRLNASIAFDGRDPREVGREYLVAKGFLKK"
     gene            10269..11603
                     /locus_tag="PLG14_03400"
     CDS             10269..11603
                     /locus_tag="PLG14_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48208.1"
                     /translation="MRAPKARPARDGEDVAGQASGRARGRAQGQAAGGRGPGSGEALP
                     EGPDFLVLAPALGSLAAALGLLPFLVLRPNRVASGTALRLAEAEPAAPLLLGAAGLLL
                     LAAALAAASAAGRRASGEPRAGSAAALAAFALAALFPPGILLALALAADRAAAGLPPA
                     GRISLGSGFWLGLAAAAALVPGLAPAFGARAGKARRFALLLGALSLLGLLAAGAFDGL
                     SLAKELLLRRATFRTELARHAAYALFPSLAALAVGLPLGRAAARSKAWERPLFLAANA
                     AQVVPTLALLGLLVAPLAALGRALPFLGVRGVGWAPASLALFLYALLPVAANARAGFR
                     MVDPAAVDAGRGMGMGRARLFFAVELPLASPALVAGFRTALAQNMGNAVLAGLIGGGG
                     LGSLVFLGLAQAAPDLVLLGALSVAAAAFAADRLLALAERTLARRVFSGGAA"
     gene            11600..12730
                     /locus_tag="PLG14_03405"
     CDS             11600..12730
                     /locus_tag="PLG14_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48209.1"
                     /translation="MIEVREATKRYPAPAGRAAARPVRGGARGAGAGPAGGAKAGGAP
                     APEGILALDGVTAAFAEGELSVLLGPSGSGKSTLLRLLNRMIEPDSGLVLIDGRDSRE
                     LDPVALRRRTGYVIQGIGLFPHLSVAENIAVVPRLLGWDSGRIRRRVGELLETLRLPP
                     SYAARRPRELSGGEAQRVGVARALAADPPVLLMDEPFGALDALTREALQAEFLRIQAE
                     LGKTIVFVTHDVGEAVRLADRIFLMRAGRLVREGRPLELLADPGEAFVADFLGRRFGI
                     ELLGREAAAPFADFGQGVGAVGGRGADGVGGGAALAGTAAPTLPRGASLGEALARMLA
                     EGRTELPIEGAEGSGVLRLDAIVRGFAPGGAGGKPRGRDARN"
     gene            12717..>13262
                     /locus_tag="PLG14_03410"
     CDS             12717..>13262
                     /locus_tag="PLG14_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48210.1"
                     /translation="MRGTRRAGGLSGRRYALFVLARRWSPPLALALLFAFAAFRFPLV
                     EAALEPVLRRKSVFVQREALGVLALQHLALVALTSGPSFLLAFPLALLAARGRARNLR
                     GLLVGAASFAETLPTVALMALLVPSLGYGFAPVAIALFLYGLLPVFRNSLSGLESTPP
                     ELVDAGLGAGMTEGELLFKVRL"
BASE COUNT         1801 a         4941 c         4526 g         1894 t
ORIGIN      
        1 gccccgcatg aagccgcccc gagccgccgc gaaagcctgg ccgtgcctcg ccctcgcctt
       61 tgccctctcg ctgtccatcc cggccgcgct atcctcctgc gccggccgcg gcgcccccgg
      121 gcagcccgcg gcgggcgggg ccgcgggccc ctccgcggac ggccagggcg gctccgacga
      181 ggcgctcctc caggcccgcg ccaaggagct ggcgctgcgc ctccccgtcg tcgacaccca
      241 catcgactat ccggaggtca ggaagcccgc ggaggacctc ctcctcggca cctcggggaa
      301 cttcgaccat ccccgcgccg tggcgggggg cctggacgcg gccttcctct cgatcttcgt
      361 cccggcggag gacgaggagg cggggaaagc cttcgcgaag gcggaggagc ggctctccct
      421 catcgaggcc gacctcgcag cccatcccga ggccctggcc ctggccgcgt cgcctgagga
      481 ggtcgcgcgc aacgcggccg cggggaggct cagcctggcc ctcggcctgg agaacgcggc
      541 cccgatcgag ggaagactcg agagggtcgc cgccttccgc aaaaggggcg tgagctacgt
      601 caccctctgc cacctcaaga acaaccatgt ctgcgactcc gcgaacgacg agtcggggcc
      661 gcggtgggga ggcctctccc ccttcggcag ggagctcgtc gccgagctca acgcgcgcgg
      721 cgtcatggtc gacctctccc atgtctcgga ggccgccttc tgggaggcct tggccctcac
      781 caaggcgccg cccatcgcct cgcactcggg cctcaggtcc ttcacccccg gcttcgagcg
      841 gaacctctcc gacgagatgc tcgccgccct ggccgcgaag ggaggcgtcg tccagattcc
      901 cttcggctcg tccttcctga gcgagaagta caggcagacg atgagctaca tggccctcgc
      961 gaccgtccgc gacgtcgccc tggtcgtcga ccgggccgtc caggtcgctg gcgtggacca
     1021 cgtggggatc ggctccgact tcgacggcgt cggcttctcc ctcccccgcg agctcaggga
     1081 cgtctcgatg taccctaacc tcgtccgcga gctcctccgc ctcggctact cggaggaggc
     1141 gatcgggaag atcctgggcg ggaacatctt gcgcgtctgg ggcgaggcga tcggggcggg
     1201 ccgcctccct tgagctttgg cctcgcatgc gctagggtag tggccacgag ccctcgatgc
     1261 gaagcccact tgtcctggtc atcctcctcg ccctctcggg cagcttctcc ttcttcctct
     1321 cgggctacgg cctctcgcgg aagcgcgtaa gcggagccct ggaattcgcc ggcttcatgg
     1381 ccgcctcggc gctctacgcc gtcggctacg cggtcgagct cacgcaaggg gagctctccg
     1441 gcatgctcgc cgtcctcatg cccgacacgg ggctcgaggg cgcggacagg gcggccgagc
     1501 gcttcaggct ggccctctcc tcggagcccg tcgcctggaa gggcgagccc atcgccgtca
     1561 cgacgagcgt cggctactgc gccgtcgtcc ccgaggaggg agagcgcctg gccgactacc
     1621 tgggccgcgc cgacctggcc ctctaccgcg ccaaggactc gggcaggaac cgctccgtcg
     1681 cctggacgcc ggcctgaggg ccgcgccggg aagtcctggc tcggctccgc acaggcggag
     1741 cgcctgcgcc ggacctcgcc gtttcggatc gaaacagcga ccggttttcc gaggcggccg
     1801 cctgtttccc ctagaaacag cgggatggga acggaggggc gccgcggcgc gggccagcca
     1861 acggacaagg gaagacaaga caacaagtta ggcgcttgaa gcagagcggg ctggctttgg
     1921 cacgctcttt gctagctaat ccgtagaacc tcagggttca tacaccacat cccaaaggag
     1981 gattgtatgg atctagcgaa atggggagcg ccggcgacca gggacctctg ggaggccttc
     2041 gacggcctgc gcggagacat ggaccgggcc ctggacttct tcagcctgcc cgacgccgcc
     2101 gggctgctcg acaggacgac ggcccccgcc gtggacatcg tcgagacgga ggaggagtac
     2161 ctcgtcgtcg ccgacctgcc cggcgtcgac aagcgcgacc tggaggtgag cgtcacgggc
     2221 tccctcctcg ccatcaaggg cgtgaagaaa gccgaaggaa attcgggcaa gcgcaaggtc
     2281 ttccgcaagg agacctgggt gggctccttc tcccggacca tcgaccttcc cgcggagata
     2341 gatccaggga agatcaaggc cgagctcaag gacggcgtcc tggcccttcg catcgcgaag
     2401 cgcgaagagg ccaagaccaa gctcatcgcc gtgaacgtgc agtgaaggag gacgccatgg
     2461 accagcggta catcgacaac gcgcgcgggg tcgccgtccc cgcctgctcg atcaccgagg
     2521 acgaggggct cgtcaccgtc aagctcgaga tgcccggagt ccccaaggag gaactcgagg
     2581 tgaggatcga gggcaacagc ctcgaggtct ccggaacccg acgccgcgcg gaggagcggg
     2641 gcaagtacct cctcagggag cgccgcgccg attccttccg caagcgcttc accctggacg
     2701 agaccatcga ccgggagaag gtcgcggccg agctcgcgga cggcatcctc gtcctcaagc
     2761 tccacatcag ggaggccgcg aagcctagga agatcgagat ctcttgaccg gccggacctg
     2821 gggcccgggg cggctggccc cgggccgccg cccgtgctat cctcaagtcc catggagacc
     2881 tcgcccctcg aagacccgag ctacctcatc cgcctcgcga ccgcgcgcat gcccttcggc
     2941 aagtacgccg gccgcctcct catcgacctc cccgagggct acgtcgtgtg gttctcccgc
     3001 cagggctacc ccgacggcga gatcggcgag ctcctcgcct ccctctacgt gatcaaggag
     3061 aacggcctcg agtcgctcct acggcccctc gtgggagggc cgcgctgaaa gcggcccggc
     3121 aggcccctcc ccttcccgcg cgagccgccg cccgcgactg aggagagggc aagtccccgc
     3181 gaggaagcat cgccctccct aaggcgccgg gaatccgacg aggccggccc cgtcgaagcc
     3241 gtccacccgg atgacttgct tcgcctggtt gtcggtgaaa tagagcctgc ccgaggaatc
     3301 gacgccgacg ccaagcgggt acatggtccg gcccccgacg ccgtcccagc cgaacacacg
     3361 ccttcccgat ccgtccatcg aggcgaagga gaacagccgt cctccgtccg cgtccgcgac
     3421 gaggatggtc ccgtcgggaa gcagcttgat gtccgccgga gcgacgaagc cgcggtccga
     3481 ggggactccc cttccgtcca agctcgtcca gccggcgccg ctcatgtcgt cgaagcggac
     3541 gatccgcccg ttcatctcgt cggccacgta gacgcggttg tcgccgtcga cggcgacggc
     3601 gaagggcagg gagaactggc cctcgccctt cccccgctcg ccaaggcgca cgaagccctc
     3661 gcctcccatg gagtcgaagc gggctacggc gtggaccgcg atgtcggtca cgtagacgcg
     3721 gccctccctg tcgacggcga cgccggcggg atagggcagg ccgtacgcgg cggtcgagag
     3781 ctccgtccag ttcccgcccc gcatgtcgtc gaagcggatc accttggcgt tcttgcaatc
     3841 cacgatgtgg atgcggccga gggaatcgaa ggcgacgcgg ccctcaagac tcaggaaccg
     3901 gcccgggccg cccgagccct tcgtgccgta gccgacgagg cccttgcccg cgatgtcgtc
     3961 catccggaaa aggcttccga ggtcccggtc cgcgaagtag atccgctggt ccgctccgac
     4021 cgcgagggcc acgggatact tgagctccgg gactttcgtc gtggcgcagg aggcgaggga
     4081 gaaggccagg agcagcgagg cggagaggga gccgaggacg cgacgtctca tgatttcctc
     4141 ctcatgatgc ttcgacaatc aagtatagcc tcggcgcgac cgcgcgcaat gaaggaccga
     4201 ggcggacggc agcggagcgg gagcgcccaa cgaggcgacc gatccgccct gccgcggctc
     4261 gctctccggc tcgatcccgg ccgctcgcgg cccggaggcg gcccgctcat tgacgcggcg
     4321 tggcggcggc actactgttt ccggaggccc catcgcgggc gcaaggagag ctcaggtgag
     4381 aggatggagc tgtatggcgc tcgggttcgc gtgcgtcctc ttggcgtcgc ccctggccgc
     4441 cgtcttcgcg gagggcgcgc cttcgggcga agcctacgcc gaggccaagg cgaaggcact
     4501 ctcctggctc gaggcccgga tggtgcccaa cgacacggtg cctgagccgg agcccgacag
     4561 gatgcgcctc ctcctgagct acgagatccc tccctccgaa cccgcctatc gctacctctc
     4621 gggccgctcc atggtctacg acgacgccct cgccgccgtc gccttcacga tggcgggaga
     4681 ctaccggaac gccgcctatg tcctcaacgc cctctcgcgc ctttcgcgcg ccgacggcgg
     4741 cctctggttc ggctacaaca cccacaactc ctggccttct gaggacgacg ccgatggcgc
     4801 cctcgaccgg acggggagcg gcgcctgggc cggctacgcc gccgtctact acatccagaa
     4861 gcgggagcgc ggggagcccg gcttcctcgc gagggacagg gaggccagga gctacctcga
     4921 gatggccaag gcgatggccg actgcctcct ttcccgcgtg gtgaaggacg ggaaggaccc
     4981 ccgagcgggc ctcgcgacgg gcggcggcgg cggctattcg ctcggttacg aggcgaagga
     5041 agtcgtggag gtcttcgcgc caggcgccgt cgactgggcg agcgcggagc acaacatcga
     5101 cctctatttc ctcctcaagg agctgggcgc gctgaccggc gagtcgaggt acctcgaggc
     5161 ggcggagacg gccaaggccg ccctcctcgg gctctggagc gcgagggacg gccagtacta
     5221 ccagggcagg aagcccgacc ggatcgacag ggtcctggcc ctggactgcg cctcctgggg
     5281 cgcgatgttc tcgcaggccg cgggcaggga ggactacgcg cgcgcctgcc tcaaggcggt
     5341 cgaggccagg tacaggagct cggacgccct cccctcctcc gtgggcggac gcgtagaggg
     5401 ctacaagccc tacgccgaca ggctcctctt cgaaggcgcg ccggaagagg tggcggagca
     5461 ttacttcggc ggcgcgaagg cgccgacctg gagggagacg gagggcgtct ggggcgaggg
     5521 ctcgctgggc gtggccctcg cctatctcaa gcaaggaaag gccggaaagg cgaaggcgat
     5581 cctcgacgag atgctgaagc tccagactcc cggcggcggc cttccctatt tcacgaggga
     5641 aatcccccat gagttcgtct cctacccgag cgtcgcgagc accgcctggc tcgcgatagc
     5701 gatctccgcc ctggaggatc ccgagggcag ctctggcttc atggggaact ggagatgagg
     5761 cgcgccggcc gcttccccgc catgcggctc gccctcgcgc tgtccctggc gctggccgcc
     5821 gccgcgacgg cctcggccca ggtgagcgca ggacaggcgg gagccggcca ggcgggcgcc
     5881 ggactgtcgg gcgctcgcct gggcaggctc tcgcgggtcg ccgtcctgga cctggacgcg
     5941 cgcaacggcg aggcgggcac ggaggccctc gcggtcaagc gctgcctcga catcctcggg
     6001 atcgcctgcc tcgtgaccgc cgaccccgcc gaggccacgc gcgaggccat ggtctgcgcg
     6061 gccggcggcc tgtggaacgg cacgctcacg tcccgggaat tttacggcct ggccgcgtgg
     6121 gtggaatccg gcggcatcct cctctcctcc ggaggcctcg ggagcaagta cttcccgctc
     6181 ttcggcatcg gccaacccgt ggactcgagg aagcgcttca gggtccgctt cgaggcgccg
     6241 gggacggatc cctcgctcgc ctacctcgac cgcccggagg agcgggaaac gcgcctcggc
     6301 gacgagaagc tcttcaagga agtcgtctgg acccgctcct ttccggtgaa gggggcgagg
     6361 agcctcgcga ggaccgagga cggcctttcc ctcttctccg tggcggacta cggcaagggc
     6421 cgggcctacg ccctcggcct ctccttcacc gacgcggtcc tcctgccgca ggtcggcggc
     6481 gactacgagg cgcagcggtc ctggatcaac ggtttcgagc cgggggcgga cgtcttcatc
     6541 ctcctcctca aggccatctt cgaggactcc ttcgggccct tcgtctacct gagcgcggtc
     6601 ccggagggca ggaagaccgc cctcctcctc tcccacgacg tggacgccca gtcctccttc
     6661 gcgcactcgg tcgatttcgc ggcgatggag cggcggaggg gcgcgaggag caccttcttc
     6721 gtcacgacaa agtacttcgc cgacgagacc gacgccgcct actgggatcc ggcccgcgcg
     6781 gccctcatcc ggaaggcgaa ggagctcggc ggcgacgtgg gctcccactc cgtcgcccat
     6841 tcgcggcgct tcggcgactt cccctcgggc gacgccgcgg tccgcaaggc ggactacgat
     6901 cccgtccgct cccccaccgt cctgggcgag ctcctcgtga gcaaggagct ccttgacgga
     6961 ggcctcccgg gccagcgcac ccgggccttc agggccggcg agctcaggat cccccgcggc
     7021 ctgggctcgg ccctggagct cgccggctac cgctacgact cctcctactc cgccaacgac
     7081 accatgacca atttcgccta ccgcatgatg cgggacagga gccccggctc cggcgagtcg
     7141 ggcatcgtgg agattcccgt caccttcgac gactcccagg gcctcctcac ggcctccaac
     7201 tggcggagcg tggtcgcggc ttggctcgac gtcgtatggg cgaacgcggc caacgaggcc
     7261 atctccgtcc tcctcatcca tcccaccgtg gccggctaca agctcgaggc cgaggagctc
     7321 ctcctcgagt ccctcgaggg caagggaatc tggatcggcg acgtctcgag ctacggcgcc
     7381 ttctggaacg agcgggccgc cctcgggtac gaagccaggc tggagggcga ccgcctggtc
     7441 gtcaggctcg gcggggagtc cctgcctccg ggacagtgcc tggtcgcggg gcggacgccc
     7501 ctcgtcaaga gcgtggaggt ccggggctcg ggcggcgccg tcctcgactg cgagacgacc
     7561 gagcgggacg ggaagctctt catcagggcg cgcggcgcga aataggcgga cggcgcagga
     7621 ctgcggcgag gcgcgaaata aggcgaacgg cgcaggggct tcccgtagcg gcgtcttgac
     7681 tgcgggcggc cgatcgtgct tccctcatgc cccatgtacg gcatcgactt cggcacctcc
     7741 aacacggtcg tcacggcgag ggcgggcggc ccggccgcgg tagcccccgc ggaaggcgcg
     7801 gccgccgacg ggacggcaag gccgggcggc cccggcggcg ccgcaagcgc cgcggccgcc
     7861 cgggtcctcg acctcggcga aggcggggtc ctcccctccc tcctctactt cgagcgcgac
     7921 aggcggccct ccatcggcgc ggaggccatg gccgactacg cggcggccct ctcgggctcc
     7981 cgcggccagg ccaacctcct ctcgcgcttc cgcttcttcc aggcgatcaa gctcgcgctc
     8041 aaggatccgt acttcgaggg gacgaggatc ttcggcgact acgtccgggt cgaggccctc
     8101 gcggccctct tcctccgcga gaccaagcgc cgcgccgacg aggccctcgc ggccgannnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggac gcgaggctcg agggccggtt
     8281 ccgcgaggcg gccctcctcg ccggcttcga ctccgtcgac ttcgtctacg agcccgtcgc
     8341 cgcctcggtg gacgccctgg acggaggcag ggggaggggg accgtcctcg tcttcgactt
     8401 cggcggcggc accctcgaca tctcggtcgc gaggggcggc gagcggggcg tcgagatcct
     8461 ctcgagcgcg ggcatggacc tgggcggcta cctcctcaac gaggacctcg cgagggcccg
     8521 gatcgtcggg catttcggcg cgggcgggaa gtggcgcacg atgacgggcc gctggctcga
     8581 gatgccggcc tggatcacga accaggtcgc ctccttctac gccctgcccc tcgccgacct
     8641 ccagcggaca aggacgacgg tgaaggagct cgtctacgac gcgaggcgcg aggacaagga
     8701 caagctgcgc ggcctcgtcg agttcctgga ccggaacctc gccttcgagc tcttcgcccg
     8761 catcgacggc gcgaagatcg agctctccgg cgcggaggag agctcgatag cctacgccgt
     8821 cccgccctac atcgagttcg aggagcggct cgcgcgcgcc gacttcgaag ccctggtcgc
     8881 gccgcgcgtg gaggccgccc gcctcctcgt cctcgaggcc ctcgcgaagg ccggcgtgga
     8941 ggcggggcgg gtcgaccgcg tcgtccgcgt cggcggctcc tcgcgggtcc ccgccttcgt
     9001 ccgcatgctc gaggggctct tctccggcag ggtcgaggag ggcgccgtct tcacgagcat
     9061 cgcctcgggc ctcatcgcgg cacgggacag gggcctcgtg gccggcgcct gagggtctgc
     9121 gcctgaggcg gacgcctgag gcaggcgctt gaggcaagcg cctagggccg gcgcgcgcgg
     9181 ccgccccgga atcgcccgat ccggttttac ttcgcgggcc cgatcgccta cacttgtcca
     9241 aggggattcc acgcggcggc ccgaggcggc cgccggcccc gcccgccccg gcgtgccccg
     9301 cggcgggcgg gcggcacaag ggggatcctc caaggagcgt gtatgcgacg cctaacttcc
     9361 ctcggattgc tcctcgccgc gacggtcctc gtcctcggcg tctcctgcgc gaaggcgccg
     9421 gaaaaggcgc cgatcgtcgt cgcctccatg atcgactccg agggggcgct cctcgggaag
     9481 atgatcgtcc aggtcctcga ggcgaacggg attccggtcc tcgacaagac cgagttcggc
     9541 acccccgacg tcctcaggaa ggcgctggag gccaaggagg tccacctcgt cgtcgactac
     9601 acgggctcgg gccagtacta ccacgaggga caggatcccg ccgtctggtc cgacgccgcg
     9661 aagggctacg aggccacgag gaggctcgac gcagcgaagg gcatagtctg gctcaagccc
     9721 tcccccgcca acaacaccga gtccctcgcg gtcaagcgct ctttcgccga aaggacgggc
     9781 gtccgggacc tgcccgcgct cgcggcctac gccaggcgcg gcggcgaggt caagctcatc
     9841 tgcgcccagt cctacgccga caatcccctc gggctcatcg gcctcgagaa ggcctacggc
     9901 ttcgagttcc gcaaggacca gctcatcctc ctctcctccg gcaacacggc tgagatgctc
     9961 aaggccctgt ccgaggggac gaacggcgtc aacgtctccc tcgtgtacgg gacggacgga
    10021 gccctcgaca agatggagct cctcgtcctg gccgacccga agagcgtgcc ccccgtctac
    10081 ctcccggcgc ccgtcatcct cgaggagaag ctcgccgaga gcccgaagat cgaggagctg
    10141 ctcgcgcccg tcttcgcctc cctggacctg gagatcctcc agcgtctcaa cgcgtccatc
    10201 gccttcgacg ggcgcgaccc ccgcgaggtc gggcgcgagt atctcgtcgc gaagggcttc
    10261 ctcaagaagt gagggctccg aaggctagac cggcccgcga cggggaggac gtcgccggcc
    10321 aggcctcggg ccgcgcgcgc ggccgagccc aaggccaagc cgcaggcggc cgggggccag
    10381 ggagcggcga ggccctcccc gagggccccg acttcctcgt cctcgcgccg gccctgggct
    10441 ccctggcggc ggccctcggc ctcctgccct tcctcgtcct caggcccaac cgcgtggcct
    10501 cggggaccgc cttgcgcctc gccgaggccg agccggcggc accgctacta ctcggcgccg
    10561 ccgggctcct cctcctcgcg gccgcgctcg cggcggcgtc cgccgccggg cggcgtgcct
    10621 ccggcgagcc gcgcgccggc tccgcggccg ccctcgcggc cttcgccctc gcggccctct
    10681 tcccgccggg catcctcctc gccctggccc tcgcggcgga cagggccgcg gctggccttc
    10741 cgcccgcggg caggatctcc ctcggctcgg gcttctggct cggcctcgcg gccgcggccg
    10801 ccctggtccc cggcctcgcg ccggccttcg gcgcccgggc cgggaaggcc aggcgcttcg
    10861 ccctcctcct tggcgccttg agcctcctcg gcctcctcgc cgccggggcc ttcgacgggc
    10921 tctcgctcgc gaaggagctg cttctgcgca gggcgacctt caggacggag ctcgcgcggc
    10981 acgcggccta cgccctcttc ccgagcctcg ccgccctcgc cgtcggcctt cccctcggcc
    11041 gggcggccgc ccgctcgaag gcctgggagc ggcccctctt cctcgccgcg aacgcggccc
    11101 aggtcgtccc gaccctcgcc ctcctcggcc tcctcgtagc ccccctggcg gccctgggcc
    11161 gcgccctgcc cttcctgggc gtgcgcggcg tcggctgggc ccccgcctcc ctcgcgctct
    11221 tcctctacgc cctcctcccg gtggcggcca acgcgcgcgc cggcttccgc atggtggacc
    11281 ccgcggcggt cgacgcgggc cggggcatgg ggatggggag ggccaggctc ttcttcgcgg
    11341 tggagcttcc cctcgcctcg ccggccttgg tcgcgggctt ccggacggcc ctcgcgcaga
    11401 acatgggcaa cgccgtcctc gcgggcctca tcggcggcgg aggcctggga agcctcgtct
    11461 tcctgggcct ggcccaggcc gcgcccgacc tggtcctcct cggcgccctc tccgtcgccg
    11521 cggccgcctt cgcggccgac cgcctcctcg ccctcgcgga gcggaccctc gcgaggcggg
    11581 ttttttcagg gggcgcggcg tgatcgaggt cagggaggcg accaagcgct accccgcgcc
    11641 cgccgggcgg gccgcggcgc gcccggtccg cggcggcgcg cgaggagccg gcgcaggacc
    11701 ggcgggcggc gccaaggcgg gcggcgctcc cgcgcctgag ggcatcctcg ccctggacgg
    11761 cgtcacggcg gccttcgccg agggggaact ctccgtcctc ctcggcccct cggggagcgg
    11821 caagtcgacc ctcttgcggc tcctcaaccg catgatcgag cccgactcgg gcctcgtcct
    11881 catcgacggg cgcgactcga gggagctcga tcccgtggcc ttgcggcggc gcaccggcta
    11941 cgtgatccag ggcatcggcc tcttccccca cctctccgtg gcggagaaca tcgccgtcgt
    12001 gccccgcctc ctcggctggg actcgggcag gatcaggcgc cgcgtcggcg agctcctcga
    12061 gacgctgcgc ctcccgcctt cctacgccgc gcggcggccc cgggagctct cgggcggcga
    12121 ggcgcagagg gtcggcgtgg cccgcgccct ggcggccgat ccgcccgtcc tcctcatgga
    12181 cgagcccttc ggcgccctcg acgcactgac gagggaggcc ctgcaggcgg agttcctccg
    12241 catccaggcc gagctcggga agaccatcgt cttcgtgacc cacgacgtcg gggaggccgt
    12301 ccgcctggcc gaccgcatct tcctcatgcg cgcgggccgc ctcgtgcgcg aggggaggcc
    12361 cctggagctc ctcgccgatc cgggcgaggc cttcgtcgcc gacttcctgg ggcgacgctt
    12421 cgggatcgag ctcctgggcc gcgaggccgc cgcccccttc gccgacttcg gacagggggt
    12481 cggcgcggtc ggcgggcgcg gagccgacgg cgtgggcggc ggggctgccc tggccggcac
    12541 cgccgcgccg acccttccgc gcggcgccag cctcggcgag gccctcgcgc ggatgctcgc
    12601 cgaaggccgg accgagctcc ccatcgaagg cgcggaaggc tcaggcgtgc tcaggctcga
    12661 cgccatcgtg cggggcttcg ccccgggcgg cgcgggcggg aagcccagag gacgcgatgc
    12721 gcggaactag gcgggccggg gggctctcgg gaaggcgcta cgcccttttc gtcctcgcgc
    12781 gccgctggtc tccgcccctc gccctcgccc tccttttcgc cttcgcggcc ttccgcttcc
    12841 ccctggtcga ggccgccctg gagccggtcc tgaggcggaa gtcggtcttc gtccagaggg
    12901 aggccctggg cgtcctcgcc ctccagcacc tcgccctcgt cgccctgacg agcggcccct
    12961 ccttcctcct cgccttcccc ctcgccctcc tcgcggcccg cggacgggcg cggaacctcc
    13021 gcggcctcct ggtcggcgcc gcctccttcg cggagaccct gccgacggtg gccctgatgg
    13081 ccctcctcgt gccgagcctc ggctacggct tcgcgcccgt cgcgatagcc ctcttcctct
    13141 acggcctcct ccccgtcttc cggaacagcc tctcgggcct ggagtcgacg ccccccgaac
    13201 tcgtcgacgc gggcctgggc gcgggcatga ccgagggcga gctcctcttc aaggtgcgcc
    13261 tg
//
LOCUS       DAOOSJ010000040        13151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3157_length_13151_cov_6.218464, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000040 DAOOSJ010000000
VERSION     DAOOSJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_13151_cov_6.218464"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLG14_03415"
     CDS             <1..691
                     /locus_tag="PLG14_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX48211.1"
                     /translation="KEADVILWDGGNNDFPFYKTDLLFTVADPHRPGNEVSFYPGEIS
                     LRMADVIVINKIDTASPEAIQTVRENIQKVNPKATVIDGASPITVDKPELIKGKRVLC
                     VEDGPTLTHGHMKIGAGIVAARKFGAAELVDPRSAAVGKLAETFKIYPNIGTLLPAMG
                     YGDEQVKDLEATINKVDCDTVVIATPIDLNRIVKINKPTVKVGYDLQEIGSPNLTEVL
                     DEFCKKNKLGK"
     gene            866..1180
                     /locus_tag="PLG14_03420"
     CDS             866..1180
                     /locus_tag="PLG14_03420"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOX48212.1"
                     /translation="MRREDEAATLAVMLGAYCARRHGGKEPCESCAELLAYARKRLAA
                     CRYGEDKPTCARCPTHCYEPGHRERIREAMRSIGPRMLFIAPRAALRHALHGLSRLRK
                     PR"
     gene            complement(1327..2916)
                     /locus_tag="PLG14_03425"
     CDS             complement(1327..2916)
                     /locus_tag="PLG14_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX48213.1"
                     /translation="MTHKDCIVVGGGIAGLTAALSLAKKGKDVLLLEKNERCGGLMNS
                     FVRDGFRFEGGARALVNAGLVKPLIKEFGLDVEVLPNPITLGVEDKLLRIDGERSLDA
                     YAGLLKGLYPGSADEVDRIIQAIRGIIEDMKVLYGVDSPLFSNKKRNALLLLPSVIAW
                     MLKFFRTMHRISRMDRPFEAYLDEISSNQSLKDIIGQHFFRKTPVFFALSYFALYNDY
                     IYPKGGVGALVQAMVDAIHAHGGEVLLDTEIQAIDARGKVLTDSGGASYGYGKMIWAG
                     DLKSLYAMTADATIPDKDRKTFARRKDAILGHRGAESVFSVYLAADAPPEEFGKVASG
                     HVFYTPDRRGLGTVHTAELQELLGRWDSVRKDEVYAWLRRFCRYNTFEISIPALRDPD
                     AAPAGKTGIIISALFEYDLAEKARAAGWHEEFKSRVEEEFIDTISSSLYPGLKDKVLF
                     SFSASPSSIRRRVGSSEGSIVGWSFEGKIPVVTSMFRMAESARTALPDVYAAGKWVYS
                     PAGGPTAIMTGRIAAKRCLKS"
     gene            complement(3034..4497)
                     /locus_tag="PLG14_03430"
     CDS             complement(3034..4497)
                     /locus_tag="PLG14_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOX48214.1"
                     /translation="MNFLEAVFVLSMLVASLACAFDKRAPRGGSLTRSWTIGMTLGGM
                     AWLAIVASGRRSWRMEPAFVSFLGLFALGVIDRIRRRGDSRDKDKDRDGGTAFAAEKP
                     RFERIARRTLGVFGVAMAMVSLAAALVFPIKNLPAPSGPHQIGTASFEVVDETRTGVY
                     LDAPGTPRRILAQAWYPADDGNSFSSLPRAPWLADSPLAKAYADYVGLPHWTMSHLRL
                     IMANGRIGAQAAAGADPMPVVIISHGWTGSRVMHTDLAEELASRGLVAIVIDHPYGAL
                     SVSFPDGTVAPLYREALPDRGTTANFGEYASALVATYAADIAAVARRVRSDATVPQLA
                     GRMDAGRIALAGHSTGGGAAVLAAMDDPAFLGVVGLDAWVEPLGYPRLERGLSTPQLH
                     IGSASWKGGVNADFLSTLKRASGPWAAYRIPGSGHADFAMVRHVTSVGAWLGLAGKED
                     GFAFARKVTPIACDWLELLLTRGAEAAFSALSGLDRE"
     gene            complement(4512..5255)
                     /locus_tag="PLG14_03435"
     CDS             complement(4512..5255)
                     /locus_tag="PLG14_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48215.1"
                     /translation="MEKSLALSLAVFLAFVPASLFAQAFLDVESGVAFTGYNDVRIPS
                     ETGDLISLKSDTDSAPAYALRVRAGYTFGGRHTVLALAAPLSVYGSGVLDRDIDYQGK
                     TFLAGTEVSSLYRFDSFRLSYRYAFVKNDSLVLAAGLTGKLRSADIAIWSDTAYAHRS
                     DLGVVPLVSLLAEWRFAGPFSLLLDADALATPFGRAEDALLALQFHASDRAAARIGYR
                     VLEGGSEGGGNVYTFALFHYLTAGLTVRF"
     gene            complement(5352..6746)
                     /locus_tag="PLG14_03440"
     CDS             complement(5352..6746)
                     /locus_tag="PLG14_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48216.1"
                     /translation="MRLTILHTNDVHAEYGPWASCVAYMKRRRAELGAEGCLTLDSGD
                     HLDMSVDECGLSGGRLHLELLADAGLDAFAPGNNEFCRAPRETLASLSLASPFPWLLS
                     TVEEEDGSAFAGFRKSVIVERGGLSVGLLGALNPFSRAAEELHGLKTLDHASALEREA
                     RALRERGAGLVVLLSHCGLDEDLGLAAETKGAIDLVVGGHSHSELFEPRRVGRTIIVQ
                     AGGHGKYVGELGLKVEGGRVEVESCALREAAALAGEDAAQAAILARWRGETDRVLSEE
                     LCVLPEALPQERLLGLAAELLRRKFGADFGMIFAPAVAGSLPAGPLRLGALYGIMRSF
                     VTPASFEISGRQVEGLLRERLDPEVTEARGFGIGFRPQGLAFGRLAFAGLDWTEGSAG
                     ASGVRVAGEPLDPERWYRAGACTHLYEAESGGYPSLDGSRNLSMTRFRYLREALAEAF
                     RSGEAERLLSELAR"
     gene            complement(6743..7867)
                     /locus_tag="PLG14_03445"
     CDS             complement(6743..7867)
                     /locus_tag="PLG14_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48217.1"
                     /translation="MSRLWVRLSAMLAGVLFFVFFLQFLAIVLDEGPGPRGAGPGGAE
                     EGRAVVRHDEGEAERAEIKRRLLEFFALSALVGLAGGFLVGRVVSRPVAEIAKAAGRL
                     GSGELSARVEPRGAKELRELALSFNSMAEGLEEAERLRKNLVADLSHELRTPLAVLDG
                     SLRAALDGVYPLDEAGIANLLGQTGHLVRLVGELRELTLAEAKKLPLRLEACVLSELA
                     GDCVQALEPLAAEKSLRLECRVPRPLVAAADPTRVRQILFNLLGNAIRHSFPGGTIVV
                     EGGSEASEPQVWLAVADSGEGLLPEELARVFERFYRADPSRSRETGGSGLGLPIALAL
                     AEAQGGSLSAASAGRGRGCTFTLRLPMIHAPAQGRGRSEP"
     gene            complement(7864..8571)
                     /locus_tag="PLG14_03450"
     CDS             complement(7864..8571)
                     /locus_tag="PLG14_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX48218.1"
                     /translation="MPQRILVVDDDPSLVKILRGYLEQAGFLVLEAGDGEAALALVRR
                     ERPDLLVLDLMLPRLGGFELTRILRSTPGLESLPVVMLTARLDEADKILGLELGADDY
                     VTKPFNPRELVARVRAVLRRAGGAQAEPEPALLTVGPLELDLKRRSLRVAGRPVELTK
                     TEFSILETLMANPGFTLGREELLEKALGYSYEGMGRTLDSHVRNLRRKIEADPESPRL
                     IETMYGVGYRLNAGGRP"
     gene            8719..9684
                     /locus_tag="PLG14_03455"
     CDS             8719..9684
                     /locus_tag="PLG14_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX48219.1"
                     /translation="MKRFLALILAAGSALALFAQAPAGAPAGMPPLGAGGPPAPVDTS
                     FVKTKYLDLAYAPVSKTQTLDIYLPNSGKGPYPVIITVHGGAFAFGDNRGAELAVGLK
                     GLERGYAVVGVNYRLSGEAVFPAAIEDVKAAIRFLRANAAKYGLDPDRFALWGSSAGG
                     NLVSLAGTSGDVAVFKNPKLGNEKVSDRVQAVVDWFGPIDFAAMDGQFKASGIAGQVH
                     DSADSFESKYLGAQITTVPDLVAKTNPATYIDAKDPPFFIQHGTADATIPTQQSRDFA
                     AKLAKAMGAGKVFFETIEGAGHGGAQFETDTNLAKVLDFLDSRLK"
     gene            9915..10499
                     /locus_tag="PLG14_03460"
     CDS             9915..10499
                     /locus_tag="PLG14_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOX48220.1"
                     /translation="MQEIRFHGRGGQGTVLASIALAKAYFAAGFQVQTFPVFGSERRG
                     APVEAYLRLDDRKILVRSNVYEPDHVMVQDMQLLGVVDVTRGLKKGGWILVNSPCLPK
                     DLKPFEGYRLAYVDAGRIALGLGLGSSTQPIINTAMMGAFARMMGAPSLEAVVEAIKA
                     EMPQKREENAAAAREAYEAVVLAGLVEPRKEAAE"
     gene            10496..12244
                     /locus_tag="PLG14_03465"
     CDS             10496..12244
                     /locus_tag="PLG14_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48221.1"
                     /translation="MTREEAASFKIPLYFSRSRVSTEANRTGNWSFVQPAYRERTAPC
                     SEACPCGTDIPRVETLASRGLFGAAYRAILMENPLPGVCGRVCFHPCEGACNRGERDE
                     AVAVNALERFLEDAARRDGAQTGLSRGPSKRKRVAVIGSGPAGLSAAHFLARLGYACE
                     IFESLPEPGGMLRWGIPAYRLPPEVLRRELDDLEALGVKISCGSKKDASFLAAPGFDA
                     VFLSPGQGKAFGLGVPGEELARDGLELLKRTRADGDGIGELKAGDRVAVVGGGNTAID
                     VARTLLRKGLKPLIVYRRRREDMPAFAHEVERALAEGAELLELSAPLALSREGPALKL
                     GLQRMRAAATGADGRRKVEAVPGETGSVAVAAVFAAVGAEPEEGWKRLLGSAPTLRLA
                     RSSLSLGPVPVAFAGDLATEEKSVSEAVASGKEAALALDAYFEGGAAAVEGRLAKARV
                     GGGPSLSMEMYLGGDRLRRGKGVVRYAELEPAYFPRAARARPAALPPKASLSSFVEVE
                     AALAPDAALAEAGRCMSCGACNDCDNCRTFCPDVASRFADGVRSVDPEYCKGCGVCAQ
                     ECPRGVMTMEREEERR"
     gene            12241..>13151
                     /locus_tag="PLG14_03470"
     CDS             12241..>13151
                     /locus_tag="PLG14_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48222.1"
                     /translation="MKEVLEGSQAAAYAVRLARAKVVSAYPITPQTHIVEELAKFCAE
                     GSLDGRFICVESEHSAMAAVIGASSGGVRTFTASSSHGLALMHEMLHWASGARLPIVM
                     AEVNRALGPGWNLWMDQTDSLSQRDTGWIQLYCEDGQEVLDTTLQAYLLAEIVNLPVM
                     VVLDAFFLSHTYEPVDVPSQAEVDAFLPPYEPKFAIDASAPCGLHEMAPPSVYMEMRR
                     SIQAAMEAVPAVFARIEEDFAARFGRGYGPVEAFQCEDAEVVLVAAGTTASTARQLVL
                     DLRAKGEKVGLLKLKMFRPFPAEALARA"
BASE COUNT         2114 a         4640 c         4506 g         1891 t
ORIGIN      
        1 gaaggaggcc gacgtcatcc tctgggacgg cggcaacaac gacttcccct tctacaagac
       61 cgacctcctc ttcaccgtcg ccgatcccca ccgccccggc aacgaggtct ccttctaccc
      121 cggcgagatc tccctccgca tggccgacgt catcgtgatc aacaagatcg acaccgccag
      181 ccccgaggcc atccagaccg tccgcgagaa catccagaag gtgaacccca aggccaccgt
      241 catcgacggc gcctccccca tcacggtgga caagcccgag ctcatcaagg gcaagcgcgt
      301 cctctgcgtg gaggacggcc cgaccctcac ccacggccac atgaagatcg gcgccggcat
      361 cgtcgccgcc cgcaagttcg gcgccgccga gctcgtcgat ccccgctccg ccgccgtcgg
      421 caagctcgcc gagaccttca agatctaccc gaacatcggc accctcctgc ccgccatggg
      481 ctacggcgac gagcaggtga aggacctcga ggccacgatc aacaaggtgg actgcgacac
      541 cgtcgtgatc gccaccccga tcgacctgaa ccgcatcgtc aagatcaaca agcccacggt
      601 gaaggtcggc tacgacctcc aggagatcgg ctcccccaac ctcaccgagg tcctggacga
      661 gttctgcaag aagaacaagc tcgggaagta agcgccgtgc cgtggccgag gacagggcgc
      721 taggcgcccg gccttcggcc taggccggcc tacgtggaga cagagccgcc cggagcgatc
      781 cgggcggctt tttgtctgtt gtcgagaaag cggaaacaga ggcgggactg ccgccgatcc
      841 cgagcgcctg gacggaagga cggaaatgag gcgagaggac gaggccgcca ccctggcggt
      901 catgctggga gcctactgcg cgaggcgcca cggagggaag gagccctgcg agagctgcgc
      961 ggagctcctg gcctacgcgc gaaaaaggct cgcggcctgc aggtacggcg aggacaagcc
     1021 aacctgcgcg agatgcccga cgcactgcta cgagcctgga caccgcgaga ggatccgcga
     1081 ggcgatgcgc tctatcgggc ccaggatgct cttcatcgcg ccccgcgccg cgctgcgcca
     1141 cgccctccat gggctgtcgc ggcttcgaaa gccgcgctaa tccgagccaa ccgttccgcg
     1201 gcgcaggctc gccgcagacg gcccagcgct cctcgggacg accttgcgcg cagcgggcgg
     1261 ctcagcgctc cgcgaggctg cttggcgcgc agccggcggt tcgggcccga ggctggaacc
     1321 ggacattcat gacttgaggc aacgcttggc cgctatcctg ccggtcatga tggctgtggg
     1381 gcctcccgcc gggctgtaca cccacttgcc ggcggcgtag acgtcgggca gggctgtcct
     1441 ggccgattcc gccatgcgga acatgctcgt cacgacgggt atcttgccct cgaacgacca
     1501 gccgacgatg gatccttccg agctcccgac ccggcggcga atcgacgacg gcgaggcaga
     1561 gaagctgaag aggaccttgt ccttgagacc cgggtagagg ctcgacgata tcgtgtctat
     1621 gaattcttcc tcgacccgcg acttgaattc ctcgtgccaa cccgccgccc gcgccttttc
     1681 ggccagatca tattcgaaga gcgcgctgat gatgatgccc gtcttgcccg cgggggcggc
     1741 gtccggatcc cgcagcgcag ggatcgagat ctcgaaggtg ttgtagcggc agaagcgccg
     1801 cagccacgcg tacacttcgt cctttcgcac cgagtcccag cgccccagga gctcctggag
     1861 ctcggcggtg tggacggtcc cgagccccct cctgtccggc gtgtagaaga cgtgcccgct
     1921 cgccaccttc ccgaactcct cgggcggcgc gtcggcggcg aggtacaccg agaagacgga
     1981 ttccgcgcct ctgtggccaa ggatcgcgtc cttgcgccgc gcgaaggtct tcctgtcctt
     2041 gtccgggatc gtcgcgtcgg cggtcatggc gtacaaggac ttcaggtctc cggcccagat
     2101 catcttgccg tagccgtacg atgcgccgcc tgaatccgta aggaccttcc cccgcgcgtc
     2161 gatcgcctgg atctcggtat cgaggaggac ctcgcccccg tgtgcgtgta tcgcgtcgac
     2221 catggcctgg accagcgccc ctaccccgcc cttggggtag atgtagtcgt tgtacagcgc
     2281 gaaatagctc agcgcgaaga acacgggcgt cttcctgaag aaatgctggc cgatgatgtc
     2341 cttcagcgac tggttggagg agatctcgtc caggtaggcc tcgaaaggcc tgtccattct
     2401 ggaaatgcga tgcatcgtcc ggaagaactt gagcatccag gcgatcaccg aaggcaggag
     2461 aaggagcgcg ttcctcttct tgttggagaa caggggacta tcgacgccgt agagcacctt
     2521 catgtcctcg atgatgcccc ggatggcctg gatgatcctg tccacctcgt ccgcgctccc
     2581 cggatacagg cctttgagca ggccggcgta ggcgtcgagg cttcgctcgc cgtcgatgcg
     2641 cagcagcttg tcctcgacgc ccagggtgat cggattgggc aagacctcga catcgaggcc
     2701 gaattccttg atcagcggct tcacaaggcc tgcgttgacc agggcgcggg cgccgccttc
     2761 gaagcggaag ccgtcgcgca cgaaggaatt catgaggccg ccgcaacgct cgttcttctc
     2821 gaggaggagc acgtccttgc ccttcttcgc gagactcaag gccgcggtaa gccccgcgat
     2881 gcccccgccg accacgatgc agtccttatg cgtcacgaca tgactccttt ctctcgccgc
     2941 ggcacggtcg ccatgaccca caaagtagca tagcgcttcc gccggcgcat ccgccagctt
     3001 cgccgcctgc ggaagccggg tcgcggcgcg cgttcattcg cggtcgagtc cggacagcgc
     3061 cgaaaaggcg gcttccgcgc ctcgggtcag gaggagctcc agccagtcgc aggcgatagg
     3121 cgtgactttc cgggcgaacg cgaagccgtc ctcttttccg gccagtccga gccaggctcc
     3181 gacgctggtg acatgccgca ccatggcgaa atcggcgtgg cccgaaccgg gtatccggta
     3241 cgccgcccag gggccggagg ccctcttcag ggtggacagg aagtccgcgt tgacgccgcc
     3301 cttccatgaa gcgctgccga tatgcagttg cggcgtggaa aggccccttt ccagtcgggg
     3361 atagccgagg ggctccaccc aggcgtccag gccgacgacg cccaagaaag ccggatcgtc
     3421 catggcggcg agcaccgccg cgccgccccc ggtggaatgg ccggccaggg cgatccgtcc
     3481 ggcgtccatc cggcctgcga gctgcggcac cgtggcgtcc gaacgtacgc gacgagccac
     3541 cgcggcgatg tcggccgcgt aggtggccac gagtgcggac gcgtactcgc cgaaattcgc
     3601 ggtggtcccg cggtccggga gcgcctcgcg gtacagcggc gccacggttc cgtccggaaa
     3661 gctgactgag agggctccgt aggggtggtc gatgacaatg gccacgagcc cgcggctggc
     3721 cagctcctcg gccaagtccg tgtgcatgac gcgagagccg gtccagccgt gcgagatgat
     3781 gacgaccggc atcggatccg cgccggccgc cgcctgggcg ccgatcctgc cgttggccat
     3841 gatgagccgc aggtgggaca tcgtccaatg ggggaggccg acgtagtccg cgtaggcctt
     3901 ggccagcggc gagtccgcca accatggcgc gcggggcagg gacgagaagg agttgccgtc
     3961 atcggcgggg taccaggcct gcgccaggat gcggcgcggc gttccaggcg cgtccaggta
     4021 gacgccggtc cgcgtctcgt ccaccacctc gaagctggcg gtgcctattt ggtgagggcc
     4081 ggaaggtgcg ggcaggtttt tgatcgggaa gaccagggcg gccgcgagcg aaaccatggc
     4141 catggcgacg ccgaagacgc cgagcgtcct gcgggcgatc ctctcgaatc gcggtttctc
     4201 cgccgcgaag gccgtgccgc cgtccctgtc cttgtccttg tctcgagaat ccccgcggcg
     4261 gcggatccgg tcgatcactc caagcgcgaa aaggcccagg aacgacacga aggccggctc
     4321 catgcgccag cttcggcgcc ccgacgcgac gatcgccagc cacgccatgc cgccgagcgt
     4381 catgccgatt gtccaggaac gggtcaagct cccgccgcgc ggcgcgcgct tgtcgaaagc
     4441 gcaggccaag ctggctacga gcatggacag gacgaaaacg gcttccagaa agttcatgcg
     4501 aggcaatccg cctagaaacg gacggtgagc cccgcggtca ggtagtggaa gagcgcgaag
     4561 gtgtagacgt tcccgccgcc ctccgagccg ccctccagga cgcggtagcc gatccgcgcg
     4621 gccgcccggt ccgaggcatg gaactggagg gcgagaaggg cgtcctcggc ccggccgaag
     4681 ggcgtcgcga gggcgtccgc gtcgaggagg aggctgaagg gcccggcgaa gcgccactcg
     4741 gccaggaggc tcacgagggg cacgacgccc aggtcgctcc ggtgggcgta tgccgtatcg
     4801 ctccagatcg cgatgtcggc gctcctgagc ttgcccgtga ggccggccgc gaggacgagg
     4861 gagtcgttct tcacgaaggc gtagcggtag ctcaggcgga aggagtcgaa gcggtagagc
     4921 gaggagacct ccgtgcccgc gaggaaggtt ttcccctggt agtcgatgtc cctgtccagg
     4981 acgccggagc cgtagacgct caagggggcg gccagggcca ggaccgtgtg gcgcccgccg
     5041 aaggtgtagc ccgcccgtac ccgcagggcg taggccggcg ccgaatccgt gtcggacttg
     5101 agggagatca ggtctccggt ctcgctcggg atgcgaacgt cgttgtagcc cgtgaaggct
     5161 acgccgctct ccacgtccag gaaggcctgg gcgaagaggg aagcgggaac gaaagcgagg
     5221 aagacggcga gggaaagggc cagcgacttt tccacgatct tctccttgtc gcgcctggcg
     5281 ccgcgtgcgg gcgcagggcc tcgttttaag atacgcatcg cgcgggaaga aggcaaaacc
     5341 ggggccgtcg cttaccgggc gagttccgac aggagccttt ccgcctcgcc cgacctgaag
     5401 gcctcggcga gggcctcgcg gaggtagcgg aaccgcgtca tcgaaagatt ccgcgagccg
     5461 tccagggagg ggtagccgcc cgactcggcc tcatagagat gggtgcaagc gccggccctg
     5521 taccagcgct caggatcgag gggctcgccg gcgacgcgga cgcctgaggc gcctgcgctg
     5581 ccttcggtcc agtccaggcc cgcgaaagcg aggcggccga aggcgaggcc ctggggcctg
     5641 aagccgatgc cgaagcccct ggcctccgtg acctcgggat cgagccgctc gcggaggagg
     5701 ccctccacct gcctccccga gatctcgaag gaggcgggcg tgacgaagct ccgcatgatc
     5761 ccgtagaggg cgcccaggcg caaggggccg gcaggcaggc tccccgcgac ggcaggggcg
     5821 aagatcatcc cgaaatcagc gccgaacttg cgccgcaaga gctcggccgc gaggccgagg
     5881 agccgctcct gcggaagggc ctcggggagg acgcagagct cctcggagag gacgcggtcc
     5941 gtctcgccgc gccagcgcgc gaggatggcg gcctgcgcgg cgtcctcgcc ggcgagggcg
     6001 gcggcttcgc ggagggcgca gctctcgacc tcgacccggc cgccctcgac cttcagcccg
     6061 agctcgccga cgtacttgcc gtggccgccg gcctggacga tgatcgtccg cccgacacgg
     6121 cgcggctcga aaagctcgct gtgggaatgc ccgccgacga ccaggtcgat cgcgcctttc
     6181 gtctccgcgg ccaggccgag atcctcgtcc aggccgcagt gcgacaggag gacgacgagg
     6241 cccgcgcccc tctcacgcag ggcccgggcc tcgcgctcca gggcggaggc gtggtcgagg
     6301 gtcttgaggc cgtgaagctc ctcggcggcg cgggagaatg ggttcagggc cccgaggagg
     6361 ccgacggaga gccctccccg ctccacgatg accgatttcc ggaagcccgc gaaggccgag
     6421 ccgtcctcct cctccacggt ggagaggagc caggggaagg gcgaggcgag gctcagggag
     6481 gcgagggtct cgcgcggcgc gcggcagaac tcgttgttgc ccggcgcgaa ggcgtccagg
     6541 cccgcgtcgg ccaggagctc gaggtggagc ctgccgccgg agaggccgca ctcgtcgacg
     6601 ctcatgtcca ggtggtcgcc cgagtccagg gtgaggcagc cctcggcccc aagctccgcc
     6661 cgcctgcgct tcatgtaggc gacgcagctc gcccagggcc cgtactccgc gtggacgtcg
     6721 ttggtgtgca ggatggtcag cctcatggtt cgctcctccc gcggccctgg gccggggcat
     6781 ggatcatagg caggcgcagc gtgaaggtac agcccctgcc ccggccagcg ctcgcggcgg
     6841 agaggctccc gccctgggcc tcggcgaggg ccagggcgat ggggaggccg aggccgctcc
     6901 cgcccgtctc gcgggagcgg gagggatccg cgcggtagaa gcgctcgaag accctggcga
     6961 gctcctcggg gaggaggccc tcgcccgagt cggccaccgc gagccatacc tgcggttccg
     7021 aggcctcgga gccgccctcg acgacgatgg tcccgcccgg gaaggagtgg cggatggcgt
     7081 tgccgaggag attgaagagg atctgcctga cgcgcgtcgg atcggcggcc gcgacgaggg
     7141 gccgcggaac gcggcactcg aggcgcaggg acttctcggc ggcgaggggc tcgagggcct
     7201 ggacgcagtc tccggcgagc tccgaaagca cgcaggcttc gagccgcagg ggaagcttct
     7261 tcgcctcggc aagggtgagc tcgcggagct cgcctacgag gcgcacgagg tggcccgtct
     7321 ggccgaggag gttcgcgatg cccgcctcgt ccaggggata gacgccgtcc agggcggccc
     7381 tgaggctgcc gtccaggacg gcgaggggcg tccggagctc gtgggaaagg tcggccacga
     7441 ggttcttccg gaggcgctcg gcctcctcga gcccttcggc catcgaattg aaggagaggg
     7501 cgagctcgcg gagctccttc gcgcccctgg gctccacgcg cgcggagagc tcgcccgagc
     7561 ccaggcggcc cgcggccttc gcgatctccg cgacgggtcg gctcacgacg cggccgacga
     7621 ggaagccgcc cgcgaggccc acgagggccg agagggcgaa gaactccagg agccgccgct
     7681 tgatctccgc cctttccgcc tcgccctcgt cgtggcggac gacagcccga ccttcctccg
     7741 ctccgcccgg ccctgccccg cgcggcccgg gcccttcatc gaggacgatg gcgaggaact
     7801 ggaggaagaa gacgaagaag aggacgccgg ccagcatcgc ggagaggcgg acccagagcc
     7861 tgctcacggc ctacctcccg cgttgaggcg atagccgacg ccgtacatcg tctctatgag
     7921 ccgggggctc tcggggtcgg cctcgatctt cctgcgcagg ttccgtacgt ggctgtccag
     7981 ggtccggccc atgccctcgt aggaatagcc caaggccttc tccaggagct cctcgcggcc
     8041 caaagtgaag ccggggttcg ccatgagggt ctcgaggatg gagaactcgg tcttcgtgag
     8101 ctccacgggc cttcccgcga cgcggaggct ccggcgcttc aggtcgagct cgaggggacc
     8161 gactgtgagc agggccggct cgggctccgc ctgggctcct ccggcgcgcc gcaggacggc
     8221 ccggacgcgg gccacgagct ctcgggggtt gaagggcttc gtgacgtagt cgtcggcccc
     8281 gagctccagg cccaggatct tgtcggcctc gtccaggcgg gcggtgagca tcacgacggg
     8341 gagggactcg aggcccggcg tcgagcgcag gatgcgggtg agctcgaagc cgcccaggcg
     8401 gggcaacatg aggtccagga cgaggaggtc gggcctctcg cggcggacca gggcgagggc
     8461 ggcctcgccg tcccccgcct ccaagacgag gaagccagcc tgctccaggt agccccggag
     8521 gatcttgacg agggaggggt cgtcgtcgac gacgaggatt cgctgcggca tggccctact
     8581 atagcaccgg cggggcctcg ggcaactcgg ccgggaagcc ccctcccgcg attcgagaat
     8641 gtatccagtt tccctcgaga attcctccag attcgtcggc tatgctttgg gcatacccta
     8701 ggaacggagg aaccgaacat gaagcgtttt ttggccctga tcctcgcggc gggctctgcc
     8761 ctcgccctat tcgcccaggc tcccgcggga gccccggccg gcatgccgcc cctgggggcc
     8821 ggcggtccgc ccgcccccgt cgacacctcc ttcgtgaaga cgaagtacct cgatctcgcc
     8881 tacgccccgg tctccaagac ccagaccctg gacatctacc tgccgaattc ggggaaaggg
     8941 ccctaccccg tcatcatcac cgtccacggc ggcgccttcg ccttcggcga caaccgcgga
     9001 gccgagctcg ccgtcggcct caagggcctc gagcgcggct acgccgtcgt cggcgtcaac
     9061 taccggctct ccggcgaggc ggtcttcccc gccgcgatag aggacgtgaa ggcggccatc
     9121 cgcttcctcc gcgccaacgc cgccaagtac ggcctggatc ccgatcgctt cgcgctctgg
     9181 ggcagctcgg ccggcggcaa cctcgtctct ctggccggca cctcgggcga cgtggccgtg
     9241 ttcaagaacc ccaagctcgg aaacgaaaag gtctccgacc gggtccaggc cgtcgtggac
     9301 tggttcggcc ccatcgactt cgcggccatg gacggccagt tcaaggcctc cggcatcgcg
     9361 ggccaggtcc acgactccgc cgactccttc gagtccaagt acctaggcgc ccagatcacg
     9421 accgtgcccg acctcgtcgc gaagacgaat cccgccacct acatcgacgc gaaggatccg
     9481 cccttcttca tccagcacgg cacggccgac gcgacgatcc cgacccagca gtcgcgcgac
     9541 ttcgccgcca agctcgcgaa ggccatggga gccggcaagg tcttcttcga gacgattgag
     9601 ggcgcgggcc acggcggggc ccagttcgag acggacacga acctcgcgaa ggtcctcgac
     9661 ttcctggaca gccggctcaa gtagccgcgt attccggccc gcccaggccc atgccgacgc
     9721 gcctgggcgg ccgctccccg ccccttcgca cttcccctcc cataaaagtt gacatttggc
     9781 gttaatggat atatcctcag ctgtcgcccg gggcccgccc cgggcgactc gcgcaaacaa
     9841 cgtctctgat acaacgaccc tccctcgacc tcacacagcc gaccagggcc tatacctact
     9901 caaagaggca tcgtatgcag gagatacgct ttcacggccg cgggggacag ggcacggtcc
     9961 tggcctccat agccctcgcc aaggcctact tcgccgccgg cttccaggtg cagaccttcc
    10021 cggtcttcgg ctcggagcgg cgcggagcac ccgtcgaggc ctacctgcgg ctcgacgacc
    10081 ggaagatcct ggtccggagc aacgtctacg agcccgacca cgtcatggtc caggacatgc
    10141 agctcctggg cgtcgtggac gtgacccggg gcctcaagaa gggcggctgg atcctcgtca
    10201 actcgccctg cctgccgaag gacctcaagc ccttcgaggg ctacaggctc gcctacgtcg
    10261 acgcgggccg gatcgccctg gggctcgggc tcggctccag cacccagccc atcatcaaca
    10321 ccgccatgat gggcgccttc gcgcggatga tgggcgcgcc ctccctggag gcggtggtcg
    10381 aggcgatcaa ggccgagatg ccgcagaagc gcgaggagaa cgccgccgcg gccagggagg
    10441 cctacgaggc cgtcgtcctg gccggcctcg tcgagccccg gaaggaggcc gccgaatgac
    10501 cagggaagag gccgcgtcct tcaagatccc cctctacttc tcgcgctccc gcgtctccac
    10561 ggaggcgaac aggacgggca actggagctt cgtccagccg gcctaccgcg agcggacggc
    10621 tccctgcagc gaagcctgtc cctgcggcac cgacatcccc cgcgtggaga ccctggcctc
    10681 gcggggcctc ttcggcgccg cctaccgcgc catcctcatg gagaatcccc tgcccggcgt
    10741 ctgcggccgg gtctgcttcc acccctgcga gggggcctgc aaccgcggcg agcgcgacga
    10801 ggccgtggcc gtcaacgccc tggagcgctt cctcgaggac gcggccaggc gcgacggggc
    10861 tcaaacgggc ctctcgcgcg gcccctccaa gaggaagcgc gtcgcggtga taggctcggg
    10921 ccccgccggc ctctcggccg cccacttcct cgcgcgcttg ggctatgctt gcgagatctt
    10981 cgagtccctt cccgagccgg gcggcatgct ccgctggggc atccccgcct accggcttcc
    11041 gcccgaggtc ctgcgccgcg agctcgacga cctcgaggcc ctgggcgtca agatcagctg
    11101 cggctcgaaa aaggatgcct ccttcctggc cgccccgggc ttcgacgccg tcttcctgag
    11161 ccccggccag ggcaaggcct tcggcctggg cgtcccgggc gaggagctcg cgcgggacgg
    11221 cctcgagctc ctcaaacgga cgagggccga cggcgacggg ataggcgaac tcaaggcggg
    11281 cgacagggtc gccgtcgtgg gcggcggcaa caccgccatc gacgtggcca ggaccctcct
    11341 ccgcaagggc cttaagcccc tcatcgtcta ccgccgcagg cgggaggaca tgcccgcctt
    11401 cgcccacgag gtcgagcggg ccctggccga gggcgcggag ctcctcgaac tctcggcccc
    11461 cctcgcccta agccgcgagg ggccggcctt gaagctcggc ctccagcgga tgcgcgcggc
    11521 cgcgaccggc gccgacggga ggcgcaaggt cgaggcggtc ccgggcgaga cgggctcggt
    11581 ggccgtcgcc gccgtcttcg cggcggtggg cgccgagccg gaggaaggct ggaagcggct
    11641 cctcggatcc gcgcccaccc tgaggctcgc ccgctctagc ctctccctgg gccccgtccc
    11701 cgtcgccttc gcgggcgacc tcgcgaccga ggagaagagc gtctccgagg ccgtcgcctc
    11761 cggcaaggag gccgccctgg ccctggacgc ctatttcgag gggggagccg cggccgtgga
    11821 gggaaggctc gccaaggccc gcgtcggcgg cggcccctcc ctctccatgg agatgtacct
    11881 tggaggcgat cgcctaaggc gcggcaaggg cgtcgtccgc tacgccgagc tggagcccgc
    11941 ctacttcccg cgcgccgcgc gggccaggcc cgccgcgctg ccgcccaagg cgagcctttc
    12001 ctccttcgtc gaggtcgagg cggccctggc gccggacgcg gccctggcgg aggccggacg
    12061 ctgcatgagc tgcggcgcct gcaacgactg cgacaactgc cgcaccttct gccccgacgt
    12121 cgcctcgcgc ttcgcggacg gggtccggag cgtcgacccg gagtactgca agggctgcgg
    12181 cgtctgcgcg caggagtgcc ctcgcggagt catgacgatg gagagggagg aggagagacg
    12241 atgaaggaag tccttgaagg cagccaggcc gcggcctacg ccgtgcggct cgcgcgcgcc
    12301 aaggtcgtct cggcctatcc catcacgccc cagacccaca tcgtggagga gctcgccaag
    12361 ttctgcgccg agggcagcct ggacggccgc ttcatctgcg tggaaagcga gcactcggcg
    12421 atggccgccg tcatcggcgc ctcctccggc ggcgtccgca ccttcaccgc gagctcctcc
    12481 cacggcctcg ccctcatgca cgagatgctc cactgggcct cgggcgcacg cctgcccatc
    12541 gtgatggcgg aggtgaaccg cgccctcggc cccggctgga acctctggat ggaccagacg
    12601 gacagcctct cccagcgcga cacgggctgg atccagctct actgcgagga cggccaggag
    12661 gtcctggaca cgaccctgca ggcctacctc ctcgcggaga tcgtcaacct ccccgtgatg
    12721 gtggtcctgg acgccttctt cctctcccac acctacgagc ccgtcgacgt gccctcccag
    12781 gccgaggtcg acgccttcct cccgccctac gagcccaagt tcgcgatcga cgcctccgcg
    12841 ccctgcggcc tccacgagat ggcgccgccc tccgtctaca tggagatgcg gcggagcatc
    12901 caggccgcca tggaggccgt ccccgcggtc ttcgcgagga tcgaggagga cttcgccgcc
    12961 cgcttcggcc ggggctacgg ccccgtggag gccttccagt gcgaggacgc cgaggtggtc
    13021 ctggtggcgg ccggcacgac ggccagcacc gcgaggcagc tcgtcctcga cctccgcgcc
    13081 aagggcgaga aggtcggcct cctcaagctc aagatgttca ggcccttccc cgcggaggcc
    13141 ctggccaggg c
//
LOCUS       DAOOSJ010000041        13006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3210_length_13006_cov_6.223689, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000041 DAOOSJ010000000
VERSION     DAOOSJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_13006_cov_6.223689"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PLG14_03475"
     CDS             complement(<1..1598)
                     /locus_tag="PLG14_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOX48223.1"
                     /translation="MVVKGDRWVDEEGRRLVLRGANLSGSAKVPLRPDGSTFLKEGFY
                     DGKGVSFVGRPLPLGEAEEHFERLARWGLRFHRLLVTWEAVEHDGPGVYDEAYLDYLE
                     ALAAKAGELGHTLFVDPHQDVWSRWTGGDGAPLWTLEGLGFRPEDFHATGAAILHQEM
                     GEAYPPMVWFTNYNRLACATTWSLFFGAEAYAPGLRVEKGLPGEGEPYADFLQGRYIA
                     AMAKVAARLSRLPNVVGFDSLNEPSAGFIGLRDLSRLERCFAKTGPMPTPWEAIQAGA
                     GFPVEVEVYGIRGTRQRAVGKTTLGEAGRRAWAEGRDCVWARAGVWGLEGGKPVLRAP
                     GHFARAGGREADVAADFLKPFIARYARAVRGAAEGGKRLAVFVEGVPNAERPSWGPKD
                     EGPVVDATHWYDDFTLGLKRWTGFLAYDSRAERLVFGPRRVRRYFKEALAELRSWSRK
                     AMGGCPTLLGEFGLPFDLNKRRAYEGRRAGDYRLHEAALSAYYDAVDESLLDSTIWNY
                     TPDNTHARGDQWNGEDLSIYCADEA"
     gene            1802..2575
                     /locus_tag="PLG14_03480"
     CDS             1802..2575
                     /locus_tag="PLG14_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX48224.1"
                     /translation="MDRGDLNGKILYDDGEHKFVWLGADPDYRRGVVQTMQYLIVDKG
                     RGWLLDPGGVHLFSRVVASASRYLPLDRIEGIFFSHQDPDVSSGIALWLGVTPAKIYI
                     SELWLRFLPHFGIVDQSRVASLEALGKGVSLGSGARLSFVPTHFMHSPGAFSLYDERA
                     RILFSGDVGAAVFPDGQESLFVDDFKAALPHLEGFHRRFIASNSVARRWSDLVARLSP
                     AMIAPQHGGVYRGQAAKDFLSWLRELRCGVDLLDELYRE"
     gene            2586..3428
                     /locus_tag="PLG14_03485"
     CDS             2586..3428
                     /locus_tag="PLG14_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48225.1"
                     /translation="MSDENYREAIDRLSAGYNKSVVLASVTNRSLEILDASIDEVEGS
                     LSSVVAAFEEIRATSRSTADNAERIDGMMGDILSKNGRTDSGVSERVAEIERAAADAK
                     RIAELFAELRGKTQRIEDVTGAIRDVSDRTNILAINASIEAARAGSVGKGFRIIANEV
                     RALSGRTGDFAKEIESTTGEFRQAVAAIDERMRGFLELLERFKASFTEVLGNFRENAA
                     SVDQAGRFLAEISGAIREENLALNEGLDSLERISGSMKDTHAVFGALTKSHAFLDELL
                     ERRS"
     gene            3425..4519
                     /locus_tag="PLG14_03490"
     CDS             3425..4519
                     /locus_tag="PLG14_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HOX48226.1"
                     /translation="MSGAPESRAAEPGDAAAGRGAGRESAPAAAKAGAPPARRPLAVI
                     TDDRFGDGASIERAVLEPAGVELAVAACSSSAEVVASCRLADALLANLAPIDAAALAG
                     LERCVVVSRYGIGLDSVDLAAARARGIAVRNVPGYCDAEVAEHAIGLILALARRIPER
                     DRLVREGAWNDAAPNRRVAGTVLGIVGFGGSGRALARAAVGFGFKAILVTSPRITAGR
                     VERGVGREFAEAARSAGCDLRPAAFEEVLTRSDWLSLNLPLLPETRGMMDAAAFARMK
                     EGACLVNTARGGLVDEAALAAALESGRLGGAALDVFAAEPLPAASPLRRLPNLVFTDH
                     SAYASAESVADLRRRTAENALEELRKAGRL"
     gene            complement(4524..5273)
                     /locus_tag="PLG14_03495"
     CDS             complement(4524..5273)
                     /locus_tag="PLG14_03495"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48227.1"
                     /translation="MKRILLALTLVLAAASLTGAFEVESLPLHLLLAGKAEAGPPLVR
                     GDHLVLSAKGPYRFVAAAFDFEGYRELHLFERNAQGVFVLAFPIPLKRREPLAYRLVV
                     DGTWMVDPRNPLKAEDRGSGLALSLAEVPYLSDERPGLYGFGAGEGRGVRFLFKGAPG
                     EIVTLAGDFNNWDPFLHELRETSPGVYELVLPLPEGSHRYAFVYRGEYLADPLNLEKA
                     TSPEGRVVSVIRVAEGGPAGAATAVSSAARR"
     gene            5375..>8649
                     /locus_tag="PLG14_03500"
     CDS             5375..>8649
                     /locus_tag="PLG14_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48228.1"
                     /translation="MPRLQDIERFKRDLAALSRESEILSRWGEVKEEPSPPEGAVAEP
                     EKPRTAPRGLAAKAAALRSAASPEAAVPDEGLPPDFASLLADLPLEEVAAESEELPPL
                     EEPEEEPAAGPEEAEELSALESFDLAAFAEPEGGAFGEETPEPQGLESFAEAEAPSEG
                     LADGFGTDLGAGFEAEALPDLEAEAPGSATAGEPALPSELESFDLPDLEAEAPGSAPA
                     EEPALPSELESFDLPDLEAEAPGSAPAEEPALPSELESFDLPDLGSEPLAEGAFELPA
                     EEAFELPSEEPSALPAEEGGPEAAGAASPGPGGDEFAIPDFDLGESPAEGLPEPPSGP
                     EKGGRAEPSSGAAGQPPSEAEPAGFDAFDSFSFEEGMTSFGEGSLGGEDLDRELASLG
                     DEAGAAETFNLDKEWGVDFEIPGEPAAAAERPAPGRRAPPPPKAPSSPRDAGAAPPRR
                     GQPSVEERLRPVALDEGQVDRLQDRLLGLPLNLRVAVEEAVANERGTEAQRSRLVWLL
                     VESAPIEEIASLAGRILQRRLAVPKGYEKSTGAAHEAEKGSFRYILAHTVIPVLRTAL
                     LVLAGAAALGWLGWKFVYRPLAANALYRSGYERIAEDRYPDAEAAFAKAGKIRDVKAW
                     YYRYAEAYAAKRQYILAERKYAALLARYPAETKGALDWARLEKDQLKFKEAAEILQKR
                     ILERAYHQKDALLLLGDVYLAWAEEEPKYYEEARRAYAALIERYGSEDAYLERMLLYF
                     VRTDKLEEVLPLKEGFMSAKKLFPSADTLAELGGYLLDKGKVDEVHRVLIAAQAKDPS
                     VPEAHYHLARYFEKAGAPAEERKALDKAVAAFARVPVLSARRLGLYVDALVRRGNFRL
                     DAREYLAAEEDFTVAADLYGKALELRRLKRSPRYAAAYAGLAEVAYWMRDDLPAALDL
                     LARAEAHGYDTQRARYQRGYALYRLGRFSESLEQFYRAGLLGESSPYLSFAFGCALYA
                     RADYHAAEGYFRRVVEAMELELERLDLPDPQGKATHGEIVELLMLAQNNLGASLYRVG
                     NRLGNPAIRSKAMAGFAESARLFDAMERDQSTMARPEAKNLGFLNMDYVLHPQRGIDL
                     AVYG"
     assembly_gap    8650..8749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9036..9107)
                     /locus_tag="PLG14_03505"
     tRNA            complement(9036..9107)
                     /locus_tag="PLG14_03505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9073..9075),aa:Val,seq:cac)
     gene            9371..9994
                     /locus_tag="PLG14_03510"
     CDS             9371..9994
                     /locus_tag="PLG14_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOX48229.1"
                     /translation="MERSLATMRASRGAAKIGEELRIELLRKGIHLLIAFVPGIARVD
                     LRLALGLLGLGTLAYAACESLRMSGVVVPLVSRVTALAARRRDAGKFVLGPVTLGLGA
                     MLALLLYPDPAASMAIYALAFGDGLSSLVGKTFGQIRLPFTKGKSLEGSLTCLTAVFV
                     SSFALTRRLGPSLALAAFATVVEAAPLKDFDNIVLPVLCGAAAVLLF"
     gene            complement(10038..10865)
                     /locus_tag="PLG14_03515"
     CDS             complement(10038..10865)
                     /locus_tag="PLG14_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48230.1"
                     /translation="MTITARRRIYWVLFAFACLGLAASAFLALPVFTGKADTSFLQAS
                     GGLPQVAFLGASLPSPSLAAAGVLLSALFSALCLGYVLYSFRKTVSSEIFFFAFWVLS
                     LAFESGRLAVFSLASGGAPTRLVLAATRLVLAGRFCGVLSIFAAGLYAAGFRNEKLGS
                     VLLFVVVIGGTLAWAVPLNTGVYEATLLVRPGYAKLLELLALLAGLATAANFVYAANS
                     TGERSYRAVALGAAALLLGQRLLVTQVAPLALALGFALLLAGAWLFVSRLHAYYLWQ"
     gene            11053..11745
                     /locus_tag="PLG14_03520"
     CDS             11053..11745
                     /locus_tag="PLG14_03520"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HOX48231.1"
                     /translation="MTKRFIFAMLVALALVLPAAAGDVATFVNLGFSADSAFFMFGQY
                     GVEAGSGRPYAELYLVDTRKNDFAPKGVQRKAYDARLEPGQDTSGALYALFAESASLA
                     KSARIDHLRPGRLLYVLLDGEEPPASLAFRDFKTEASFDVSLQKSVAESKGSVVSSFG
                     LSVAVKAKDGRSRSVTGGNPGIKRTGVKDYVIRRIIAAPDEKTLVLIIEKRLADKGDA
                     SVRYMVETVKLP"
     gene            11921..12844
                     /locus_tag="PLG14_03525"
     CDS             11921..12844
                     /locus_tag="PLG14_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48232.1"
                     /translation="MSSSGRAGRIGARGARALAAAAGAAALVFLLAGVPILVTAKDGM
                     PVLAPKALLEAMAGWARGLSDGSSFRYEIGSTSWSLLEEAPLFLAVSFANLAVPGAAG
                     VFLGALAGTVLRPRRRGWPDRAVDALAATPDFIVALAAQVVAIGLLAPLGFRIRIGPG
                     RSGLSLLGLAVMGIYPFCFAYRAAARASRAVEGSDYVAYARAKGLPEREILRRHVGAA
                     VAPALANELPTILSFMQGTLFIVEHLFGLPGVARFLFSAAFSGRRRGFYEVYQYDLGV
                     FVLAGLGLAYALVYAFVRLALALARKALAHE"
BASE COUNT         1854 a         4810 c         4465 g         1777 t
ORIGIN      
        1 gcctcgtcgg cgcagtagat ggacaggtcc tcgccgttcc actggtcgcc ccgggcgtgc
       61 gtgttgtcgg gcgtgtagtt ccagatcgtc gaatcgagga ggctttcgtc caccgcgtcg
      121 tagtaggccg agagggccgc ctcatgcaag cggtagtcgc cggcccgccg gccctcgtag
      181 gcccgccgct tgttgaggtc gaagggcagg ccgaattccc cgagaagggt cgggcagccg
      241 cccatcgcct tccggctcca ggagcggagc tcggcgaggg cctccttgaa gtagcgcctg
      301 acgcgccggg ggccgaagac gaggcgctcg gcgcgggagt cgtaggcgag gaagcccgtc
      361 cagcgcttca ggcccagggt gaagtcgtcg taccagtgcg tcgcgtcgac gaccggcccc
      421 tcgtccttgg gcccccagga gggcctctcg gcgttcggca cgccttcgac gaagacggcg
      481 agccgctttc cgccctccgc cgcgccccgc acggcccggg cgtagcgcgc gatgaagggc
      541 ttgaggaagt cggccgcaac gtccgcctcg cggccgcccg cccgggcgaa atggccggga
      601 gccctgagga cgggcttccc gccctcgagg ccccagacgc cggcgcgggc ccagacgcag
      661 tcccgcccct cggcccaggc gcggcgtccc gcctcgccga gcgtggtctt ccccaccgcg
      721 cgctggcggg tccccctgat gccgtagacc tcgacctcca cggggaagcc cgcgccggcc
      781 tggatcgcct cccagggcgt cggcatcggg ccggtcttcg cgaagcagcg ctcgagccgg
      841 gagaggtccc tgaggccgat gaagccggcg gagggctcgt tcaggctgtc gaagccgacg
      901 acgttcggaa gcctgctgag ccgggccgcc accttggcca tcgcggcgat gtagcggccc
      961 tggaggaagt ccgcgtaggg ttcgccctcg cccggcagtc ccttctccac gcggaggccg
     1021 ggagcgtagg cctcggcgcc gaagaagagg ctccacgtcg tggcgcaggc caggcggttg
     1081 tagttcgtga accagaccat cggcggatag gcctcgccca tctcctggtg gaggatggcc
     1141 gcgccggtcg catgaaaatc ctcgggccgg aagcccagcc cctccagggt ccacagcggc
     1201 gccccgtcgc cgcccgtcca gcggctccac acgtcctggt gggggtcgac gaagagggtg
     1261 tggccgagct cgccggcctt cgcggccagg gcctccaggt agtcgaggta ggcctcgtcg
     1321 tagacgccgg gtccgtcgtg ctccaccgcc tcccaggtga cgaggagccg gtggaagcgg
     1381 aggccccagc gcgcgagccg ctcgaagtgc tcctccgcct ccccgagggg gaggggacgg
     1441 ccgacgaagg agacgccctt cccgtcgtag aagccttcct tcaggaaggt cgagccgtcg
     1501 ggcctaagcg gcaccttcgc gctgcccgag aggttcgcgc cccggaggac gagccgccgg
     1561 ccctcctcgt ccacccacct gtcgcccttc acgaccatcg ccgcatgcct ccctgggcgc
     1621 gcggacctcg agggcccgct gcgccatgac gccagtttag cataggctaa accggcgggc
     1681 ggcgcgaaaa tgcggtccgc gagggaaagc cgcgttccgc ggggaaggcc ggagcttcgc
     1741 gcgggaagtc gacgggcccc gccgcttccg ctatactggg actccgcaag ggggagacat
     1801 gatcgaccgc ggcgacctca acggaaagat cctctacgac gacggcgagc acaagttcgt
     1861 ctggctcggg gccgaccccg actatcggcg cggggtcgtc cagaccatgc agtacctcat
     1921 cgtggacaag ggacggggct ggctcctcga tccgggcggg gtccacctct tctcccgcgt
     1981 cgtcgcgagc gcgagccgct acctcccgct cgacaggatc gagggcatct tcttctcgca
     2041 ccaagacccc gacgtctcct ccggcatagc cctctggctc ggcgtcacgc cggcgaagat
     2101 ctacatctcc gagctctggc tccgcttcct gccccacttc ggcatcgtgg accagtccag
     2161 ggtcgcctcc ctcgaggccc tgggcaaggg cgtctccctg ggctcgggcg ccaggctctc
     2221 cttcgtcccg acccacttca tgcactcgcc gggggccttc tccctctacg acgagcgcgc
     2281 gaggatactc ttctcgggcg acgtcggcgc ggccgtcttc cccgacggcc aggaaagcct
     2341 cttcgtcgac gacttcaagg cggccctccc ccacctcgag ggcttccacc gccgcttcat
     2401 cgcctccaac tccgtcgccc gccgctggtc ggacctggtc gccaggctct cgcccgcgat
     2461 gatcgcgccc cagcacggcg gcgtctaccg cggccaggcc gcgaaggatt tcctgtcctg
     2521 gctcagggag ctccgctgcg gcgtcgacct cctcgacgag ctctacaggg aataggaggc
     2581 gacccttgtc cgacgagaac taccgcgagg ccatagaccg cctctccgcg ggctacaaca
     2641 agagcgtcgt cctcgcctca gtgacgaacc gctccctcga gatcctcgac gcctcgatcg
     2701 acgaggtcga gggatccctc tcctccgtcg tcgccgcctt cgaggagatc cgcgcgacga
     2761 gccggagcac ggccgacaac gcggagcgca tcgacgggat gatgggcgac atcctctcca
     2821 agaacggccg caccgactcg ggcgtctccg agcgggtcgc ggagatcgag agggcggccg
     2881 cggacgcaaa gcgcatcgcg gagctcttcg ccgagctccg cggcaagacc cagcgcatag
     2941 aggacgtgac gggcgcgatc cgcgacgtgt cggaccgcac gaacatcctc gccatcaacg
     3001 cctccatcga ggcggcgcgc gccggctccg tcgggaaggg cttccgcatc atcgcgaacg
     3061 aggtgcgggc cctctccggg cggacgggcg acttcgcgaa ggagatcgaa tcgacgaccg
     3121 gcgagttccg gcaggccgtg gccgcgatcg acgagcggat gcgcggcttc ctcgagctcc
     3181 tggagcgctt caaggcctcc ttcaccgagg tcctcggcaa cttccgggag aacgccgcga
     3241 gcgtcgacca ggcgggccgc ttcctcgccg agatctcagg cgcgatcagg gaggagaacc
     3301 tggcgctgaa cgaggggctc gactccctgg agcgcatctc cggctcgatg aaggacaccc
     3361 acgccgtctt cggcgccctc acgaagagcc atgccttcct ggacgagctc ctggagaggc
     3421 gctcgtgagc ggcgccccgg aatcgcgcgc ggcggagccg ggcgacgccg ctgccggacg
     3481 cggcgcgggc cgcgaaagcg ccccggccgc ggccaaggcg ggcgcccccc ccgcccggcg
     3541 ccccctcgcc gtcatcaccg acgaccgctt cggcgacggc gcgtcgatcg agcgcgccgt
     3601 cctcgagccg gcgggggtcg agctcgccgt cgcggcctgc tcgagctcgg ccgaggtcgt
     3661 cgcctcctgc cgcctcgcgg acgccctcct cgcgaacctg gcgcccatcg acgcggccgc
     3721 cctggcgggc ctggagcgct gcgtcgtggt gtcgcgctac gggatcggcc tcgactccgt
     3781 cgacctggcc gcggcccggg cgcgcggcat agccgtgcgc aacgtccccg gctactgcga
     3841 cgccgaggtc gccgagcacg ccatcggcct catcctggcc ctggcccggc gcatccccga
     3901 gcgcgaccgc ctcgtccgcg agggcgcctg gaacgacgcc gcgccgaacc gccgcgtggc
     3961 gggcaccgtc ctcggcatcg tcggcttcgg cggctcgggg cgggccctgg cccgcgccgc
     4021 ggtcggcttc ggcttcaagg ccatcctcgt cacgagcccc cgcatcacgg ccggcagggt
     4081 cgagcggggc gtcggcaggg agttcgccga ggcggcgcgc tcggcgggct gcgacctccg
     4141 gcccgccgcc ttcgaggagg tccttacgcg ctccgactgg ctttcgctca acctgcccct
     4201 ccttccggag acgcggggca tgatggacgc ggcggccttc gcgcggatga aggagggcgc
     4261 ctgcctcgtc aacacggcgc gcggcggcct cgtcgacgag gcggccctcg cggcggccct
     4321 ggaaagcggc cgcctgggcg gcgccgccct cgacgtcttc gccgccgagc ccctccccgc
     4381 ggccagcccc ctgcggcgcc ttccgaacct cgtcttcacc gaccactcgg cctacgcctc
     4441 cgcggagtcc gtcgccgact tgcggcggcg cacggcggag aacgcgctgg aggagctcag
     4501 gaaggcgggg aggctctagg gagtcagcgc ctcgcggcgg aggagacggc cgtcgccgcg
     4561 ccggcggggc ctccctccgc cacgcggatg acggagacga cgcggccctc gggcgaggtg
     4621 gccttctcca ggttgagcgg atcggcgagg tactcgccgc ggtagacgaa ggcgtagcgg
     4681 tggctcccct cgggcagggg caggacgagc tcgtagacgc cgggagaggt ctcgcggagc
     4741 tcgtggagga agggatccca gttgttgaag tcgcccgcca gggtgacgat ctcccccgga
     4801 gcgcccttga agaggaagcg gacgcccctc ccctccccgg cgccgaagcc gtagagcccc
     4861 ggacgctcgt ccgagaggta gggcacctcc gcgagcgaga gggccaggcc cgagccgcgg
     4921 tcctccgcct tgagcgggtt gcgggggtcg accatccagg tcccgtccac gacaagccgg
     4981 taggccaggg gctcgcggcg cttgagcggt atggggaagg cgagcacgaa gacgccctgc
     5041 gcgttcctct cgaagaggtg cagctcgcgg tagccttcga agtcgaaggc ggcggcgacg
     5101 aagcggtagg gccccttggc cgagaggaca aggtggtcgc cgcgcacgag gggcgggccg
     5161 gcctcggcct tccccgcgag gaggaggtgg aggggcaggg actcgacctc gaaggccccg
     5221 gtcagggacg cggctgcgag gacgagcgtg agggcgagga gtatgcgctt catagggcct
     5281 tcgtactatg gatatcggcc ccgccgcccg gcctcttgaa gctaatttcc tatacaaacg
     5341 ggcgattgtt ccgttaatat aggctaggac taccatgccg cggctgcagg acatcgaaag
     5401 gttcaagcgc gatctcgccg ccctctcgcg ggaatcggag atcctctccc gttggggcga
     5461 ggtgaaggag gagccgtctc ctcccgaagg cgccgtcgcc gagcccgaga agccgcggac
     5521 agccccccgc ggcctcgccg ccaaggccgc cgccctccgc tccgccgcct cgcccgaggc
     5581 cgccgtcccc gacgagggcc tccctcccga tttcgcgagc ctcctcgccg atctccccct
     5641 ggaggaggtg gccgccgagt ccgaggagct gccgccgctc gaggagcccg aggaggagcc
     5701 cgccgccggc cccgaggagg ctgaggagct ctccgccctg gaaagcttcg acctcgccgc
     5761 cttcgccgag cccgagggcg gagccttcgg ggaggagacg cccgagcccc agggcctcga
     5821 gtcctttgcc gaggccgaag cgccctccga gggcctggct gatggcttcg ggacggacct
     5881 tggagccggc ttcgaggccg aggccctccc cgacctggag gccgaggcgc cgggttcagc
     5941 gacggccgga gagccggccc tcccctccga gctcgagagc ttcgacctcc ccgacctcga
     6001 ggccgaggcg ccgggttccg cgccggccga agagcctgcc ctcccctccg agctcgagag
     6061 cttcgacctc cccgacctcg aggccgaggc gccgggttcc gcgccggccg aagagccggc
     6121 cctcccctcc gagctcgaga gcttcgatct ccccgacctg ggctccgagc ccctggccga
     6181 gggggctttc gagcttcccg cggaggaggc cttcgagctt ccgtccgagg agccgtcggc
     6241 cctgcccgcg gaagagggcg gtccggaggc ggccggcgcc gcgtctccgg gcccgggcgg
     6301 cgacgagttc gcgatacccg acttcgatct cggggagagc cccgccgagg gcctccccga
     6361 gcccccctcg ggccccgaga agggcggccg ggccgagccc tcgtccggcg ccgccggcca
     6421 gcccccctcc gaggccgagc cggccggctt cgacgccttc gactccttca gcttcgagga
     6481 gggcatgacc tccttcggcg agggaagcct gggcggcgag gacctggacc gggagctcgc
     6541 ctccctgggc gacgaggccg gcgccgcgga gaccttcaac ctcgacaagg aatggggcgt
     6601 cgacttcgag attcccggcg agccggcggc cgcggccgag cggcccgcgc cggggcggcg
     6661 cgccccgccc ccgcccaagg ccccctcctc gccccgggac gcgggcgccg ccccgccccg
     6721 ccgcggccag ccctccgtgg aggagcgcct ccgcccggtg gccctggacg agggccaggt
     6781 ggaccgcctc caggaccgcc tcctcggcct gcccctcaac ctgcgcgtcg ccgtggaaga
     6841 ggcggtggcc aacgagcggg gcacggaggc ccagcgctcc cgcctcgtct ggctcctcgt
     6901 cgagtcagcg ccgatagagg agatcgcctc gctcgcgggg cggatactcc agcggcgcct
     6961 cgccgtcccc aagggctacg agaagagcac cggcgccgcc cacgaggcgg agaagggcag
     7021 cttccgctac atcctcgccc acacggtcat ccccgtcctc aggaccgccc tcctcgtcct
     7081 cgcaggcgcg gcggccctgg gctggctggg ctggaaattc gtctaccgcc ccctcgccgc
     7141 gaacgccctc taccgctcgg gctacgagcg catcgcggag gaccgctacc ccgacgccga
     7201 ggccgccttc gccaaggccg gcaagatcag ggacgtgaag gcctggtact accgctacgc
     7261 ggaggcctac gcggccaagc gccagtacat cctcgcggag cggaagtacg cggctctcct
     7321 ggcccgctat cccgcggaga ccaagggagc cctggactgg gcgcgccttg agaaggacca
     7381 gctcaagttc aaggaggccg cggagatcct gcagaagcgg atcctggagc gggcctacca
     7441 ccagaaggac gccctcctcc tcctgggcga cgtctacctc gcctgggccg aggaggagcc
     7501 gaagtactac gaggaggcgc ggcgcgccta cgccgccctc atcgagcgct acggctcgga
     7561 ggacgcctat ctcgagcgca tgctcctcta cttcgtcagg accgacaagc tcgaggaagt
     7621 cctccccctc aaggaaggct tcatgtcggc caagaagctc ttcccctcgg ccgacaccct
     7681 cgccgagctc ggcggctacc tcctcgacaa gggcaaggtc gacgaggtgc accgcgtcct
     7741 catcgcggcc caggccaagg atccctcggt gccggaggcg cactaccacc tggcccgcta
     7801 cttcgagaag gccggggccc ccgcggagga gcggaaggcc ctcgacaagg ccgtggcggc
     7861 cttcgcccgc gtgcccgtcc tgtcggcgcg gcgcctcggc ctctacgtcg acgccctcgt
     7921 gcgccgcggg aatttccgcc tcgacgcccg cgagtacctc gcggcggagg aggacttcac
     7981 ggtcgccgcc gacctctacg gcaaggccct ggagctcagg cgcctcaagc ggagcccccg
     8041 ctacgccgcg gcctacgcgg gcctggcgga ggtggcgtac tggatgcgcg acgacctgcc
     8101 cgccgccctc gacctcctgg cccgggcgga ggcccacggc tacgacaccc agcgcgcgcg
     8161 ctaccagcgc ggctacgccc tctaccgcct gggccgcttc tccgagtccc tggagcagtt
     8221 ctaccgcgcc ggcctcctgg gcgagtcgag cccctacctc tccttcgcct tcggctgcgc
     8281 cctctacgcc cgcgccgact accacgccgc ggagggctac ttccgccgcg tcgtcgaggc
     8341 gatggagctg gagctcgagc gcctggacct gcccgatccc caaggcaagg ccacccacgg
     8401 cgagatcgtc gagctcctca tgctcgcgca gaacaacctg ggcgcctcgc tctaccgggt
     8461 gggcaaccgc ctggggaatc cggccatccg ctccaaggcc atggcgggct tcgcggagtc
     8521 agcgaggctc ttcgacgcca tggagcgcga ccagtcgacg atggcgcgcc ccgaggcgaa
     8581 gaacctcggc ttcctcaaca tggactacgt cctccatccc cagcgcggca tcgacctggc
     8641 cgtctacggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt caacatggac
     8761 tacgtcctcc atccccagcg cggcatcgac ctggccgtct acggcgccat cgagcgcgac
     8821 atgcgcttcc cgaagcagcg ctgaagcggc gcgggagcgg cgcgggagcg gcgcgggagc
     8881 ggcgcgggag cggcgctgaa gcggcacggc gcaagggcgg gcacagcgca ggcgcggcgc
     8941 gggcgcgacg cgggcgcggg cgcggttgga ggccgagggg catgaggctg gtccgggttc
     9001 gcttggaggg ggcggtttaa aatagaaaag tccggtgggc gatattgggt ttgaaccaat
     9061 gacttctacc gtgtgaaggt agcactctcc cgctgagtta atcgcccacc ggactttccg
     9121 gacggctccc gggaggggcc gcaggaagga atctagcatc gggacccttc actgtcaagg
     9181 cgcgggcccg gccaattcct tgatcgccgc tgtcgagtaa atcatacgcc cgcgcggcgg
     9241 cgcttttcaa gaccgggcta tatgcctaga atgaatggag atagccgcgc cgatccggag
     9301 cgacgaatct ggcatggttc ttgcattaaa gattgaatag acaaacgaac ggatttgaag
     9361 gggatgcgcc atggagagaa gcctcgccac gatgagggcc tcgaggggag ccgccaagat
     9421 aggcgaagag ctcaggatcg agctcctccg caagggcatc catctcctca tcgccttcgt
     9481 gccgggcatc gcgcgcgtcg acctccgcct ggccctcggc ctcctgggcc tcgggaccct
     9541 ggcctacgcc gcctgcgagt ccctgcgcat gtcgggcgtg gtcgtgcccc tcgtctccag
     9601 ggtcaccgcc ctcgcggcca ggcgccgcga cgccggcaag ttcgtcctcg gccccgtcac
     9661 cctgggcctg ggcgcgatgc tcgccctcct cctctatccc gatcccgcgg cctccatggc
     9721 catctacgcc ctggccttcg gcgacggcct ctcgagcctc gtcggcaaga ccttcggcca
     9781 gatccgcctc cctttcacga agggcaagtc gctggagggc agcctcacct gcctgacggc
     9841 cgtcttcgtc tcttccttcg ccctgacgcg gcgcctcggc ccctccctgg ccctggccgc
     9901 cttcgcgacg gtcgtggagg ccgcccccct caaggacttc gacaacatcg tcctgcccgt
     9961 cctctgcggc gcggccgccg tcctcctctt ctagccttcc tcagcccggc cccggcccgc
    10021 ggcggcagcc gcgccggcta ttgccagagg tagtaggcgt ggaggcggga gacgaaaagc
    10081 caggcgcccg ctagaagcag ggcgaagccg agggccaggg cgaggggcgc cacctgcgtg
    10141 acgaggaggc gctggcccag caggagggcc gcggcgccca gggccacggc ccggtaggag
    10201 cgctcgcccg tcgagttcgc cgcgtagacg aagttggccg ccgtggccag ccccgcgagc
    10261 agggccagga gctcgaggag cttggcgtag cccggcctca cgaggagggt agcctcgtag
    10321 acccccgtgt tgagggggac ggcccaggcc agggtgccgc cgatgacgac cacgaagagg
    10381 aggaccgagc cgagcttctc gttgcggaag cccgccgcgt agagtccggc cgcgaagatc
    10441 gacaggacgc cgcagaagcg ccccgcgagg acgaggcgcg tcgcggccag gacgaggcgg
    10501 gtgggcgctc cgcccgaggc gaggctgaac acggcgagcc ggcccgactc gaaggcgagg
    10561 gagagcaccc agaaggcgaa gaagaagatc tccgaggaga cggtcttcct gaaggaatag
    10621 aggacatagc ccaggcagag ggctgagaag agggccgaga ggaggacgcc ggccgcggcc
    10681 aggctcggcg agggcaggga agcgccgagg aaggcgacct gggggaggcc gcccgaggcc
    10741 tggaggaagg aagtgtccgc cttgcccgtg aatacgggca gggccaggaa ggccgaggcc
    10801 gccaggccca ggcaggcgaa ggcgaacagc acccaataga tccggcgtct ggcggttatc
    10861 gtcacggcat tgagcatagc gcggcctcgg ggctttggaa agtcgtcgcg gggccggccg
    10921 aggacttgct gcgccgccgc cgcggctcgg cccgcgccgt ccgcccgcgc cgccgtgggc
    10981 cgccgcggac cgtccgcgct ttccggtaag gcccgggccc ctcccggccg atactgtcgc
    11041 taggagcccg ccatgacgaa acgcttcatt ttcgcgatgc tcgtagcgct cgccctcgtc
    11101 ctgccggccg cggccggcga cgtcgcgacc ttcgtgaacc tcggcttctc cgccgattcg
    11161 gccttcttca tgttcggcca gtacggggtc gaggccggaa gcggcaggcc ctacgccgag
    11221 ctctacctcg tcgacaccag gaagaacgac ttcgccccca agggcgtcca gcgcaaggcc
    11281 tacgacgcca ggctcgagcc gggccaggac acgtcgggcg ccctctacgc gctcttcgcc
    11341 gagtccgcat ccctcgccaa gtccgccagg atcgaccacc tcaggccagg ccgcctcctc
    11401 tacgtcctcc tggacgggga ggagccgccc gcctcgctcg ccttccgcga cttcaagacc
    11461 gaggcctcct tcgacgtctc cctccagaag agcgtcgccg agtccaaggg gagcgtcgtc
    11521 tcctccttcg gcctctccgt cgccgtgaag gcgaaggacg gccgctcccg ctccgtgacg
    11581 ggcggcaacc ccggcatcaa gcggacgggc gtgaaggact acgtgatcag gcggatcatc
    11641 gccgcccccg acgagaagac cctcgtcctc atcatcgaga agcggctcgc cgacaagggc
    11701 gacgcctcgg tgcgctacat ggtggagacg gtcaagctcc cctagaaagg caagtgcccg
    11761 aaggcgggat ccccgactcg ccctcgccgc tcccccgcga tccgggggag cggatttttt
    11821 ggagccgcct gggtgcccgg cggcggctcg gggcttcgac gccggccttg gcgcggcgcc
    11881 ggcccgaggc ggagcgggaa aggaaggatt cgtgcgcgac atgtcgtcgt cgggacgggc
    11941 cgggaggata ggcgcccggg gggcgagggc cctggccgcc gccgccggcg cggccgccct
    12001 cgtcttcctc ctcgccggcg tcccgatcct cgtcaccgcg aaggacggga tgcccgtcct
    12061 cgcgcccaag gccctcctcg aggccatggc cggctgggcc cgcggccttt ccgacggcag
    12121 ctccttccgc tacgagatcg ggtccacgag ctggagcctc ctcgaggagg ctcccctgtt
    12181 cctggccgtc tccttcgcga acctggccgt cccgggcgcc gcgggcgtct tcctgggcgc
    12241 cctcgcgggc acggtcctgc gcccgcgccg caggggctgg cccgaccggg ccgtcgacgc
    12301 cctggccgcg acccccgact tcatcgtcgc cctcgccgcg caggtcgtcg ccatcggcct
    12361 cctcgctccg ctcggcttca ggatcaggat aggaccggga cgctcgggcc taagcctcct
    12421 cggcctcgcc gtgatgggga tctacccctt ctgcttcgcc tatcgcgccg cggcgcgggc
    12481 ctcgcgcgcc gtggagggct cggactacgt cgcctacgcc cgggccaagg gcctccccga
    12541 gcgggagatc ctcaggcgcc acgtgggggc ggccgtcgcg cccgccctgg ccaacgagct
    12601 cccgacgatc ctctccttca tgcagggcac gctcttcatc gtcgagcacc tcttcggcct
    12661 gcccggcgtc gcgcgcttcc tcttctcggc ggccttctcg ggccggcgcc gcggcttcta
    12721 cgaggtctac cagtacgacc tcggcgtctt cgtcctcgcg gggctcggcc tggcctacgc
    12781 cctcgtctac gccttcgtcc ggctggccct ggccctggcc aggaaggcgc tggcccatga
    12841 ataggcgact cgccgcgggc gtcgccctcc tcgccctcct cctcgcggcc tccctcctcg
    12901 cgccgctgta cgcgcccttc gagcccggcc atacggagag cctccgccgg gtggagaggg
    12961 acggcgaggt cgcctacctc ttctctcccg aggcgcccaa cgagag
//
LOCUS       DAOOSJ010000042        12947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3234_length_12947_cov_6.433913, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000042 DAOOSJ010000000
VERSION     DAOOSJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_12947_cov_6.433913"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLG14_03530"
     CDS             complement(<1..802)
                     /locus_tag="PLG14_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOX48233.1"
                     /translation="MADDRTKKEERAETKAAVTQSAAHEAAETAEDGAAAERALSEQA
                     RIRREKLAKLQAAGKDPFAVVKYDQTHHSLQIKEGFESLDGAEVSVAGRIMSFRDIGK
                     ASFVDLQDRDGRIQIYLNADHLGAEPYAELKTLDMGDIIGVKGKVFKTRRGEISVEAA
                     SVTLLTKALEPLPEKFHGLKDTEVRYRRRYLDLVMNRETMDTFRKRTKIIASIREYLD
                     SHEFIEVETPILSTIASGAAARPFTTKSNALNLQLYLRIATELYLKRCI"
     gene            complement(1031..3679)
                     /locus_tag="PLG14_03535"
     CDS             complement(1031..3679)
                     /locus_tag="PLG14_03535"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HOX48234.1"
                     /translation="MNPHELPVYQQKERILEVLKDHQVVVVESPTGSGKTTQLPIILH
                     EAGYSKFGLIGVTQPRRIAALSVSDFIARQIGTTVPGVVGYKMRFEDKTNDSTAIKIM
                     TDGILLQEMKLDPALSKYSVLMVDEAHERSLNIDFILGLVKRVIEARPEFKVIVSSAT
                     INAEVFSEYFGQCPIVKIETPMYPVTVVYDPAQAAPEDEGSRFAAEDALQAKIEEIVG
                     RIVGERREGDILVFLPGEKAIKDCMKRLAFGPVGDRLHVIPLYARLGKDEQERVFEVP
                     PPGKTKVVISTNIAETSLTIDGITSVIDSGLAKLNYYNPKSFTSSLIETPISKASCNQ
                     RKGRAGRTRPGVCYRLYGRSDFEGRPLFTTEEIYRTDLTEVVLRMAELGISEFERFEF
                     ISQPPKSGILGAEEALALLDALTPERGLTRVGEMMCAFPLLPRHSRIIVEAVLQYPDV
                     VEEALVGTSFLSTTSPYVLPPGEEMEARRAHHAFRDPSGDFVSYLKIFAAFREARDKA
                     KFCERSYLDERTMLEIFRIKEQLELIVSGLGVPIVGGGRCEDYLCAVSRGLIQFVCAR
                     QDRGLFRSLTAEKIQIHPGSVMYKENPNYIVAGEIVRTTRMYAMSVSPLEKAWLSRIS
                     PRLEGLLRGGYVSAAAGRSREEGYAERGRPGERPGERHRLERGEADRRSGRHGRREED
                     EALAEPAAGAERPVRDFTNKIKIGSEVFDLAVDKKKKKVVSLEWQKLAKVREELDRES
                     LAVYKGMKGVVLVGHRKLLEGEKLDLILRLAPSLDPERDLARDWPRKKNFDSGEGLHE
                     LLASLDHVLQVARAKDQSAELGFVALFTDSEGNYWFRCSRGFHTALNESVASLDALAD
                     ALGEGASPEEKEKVSALYRKLTSFFE"
     gene            3750..5072
                     /locus_tag="PLG14_03540"
     CDS             3750..5072
                     /locus_tag="PLG14_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOX48235.1"
                     /translation="MRELFTDVAVLAALAAIAAGRLPRLRMNRATIALAAAALLVAAG
                     SIGLEEAFAAIDLGTVCLLLSMMVIVANLRLSGFFEAAGAKLLAAARGPRQLLALVVL
                     ASGFLAALFLNDTVCLMLTPLVVELALRSRRDPLPYLVALATAANVGSAATIVGNPQN
                     MLIGASSGIPFGRFLLMLGPPSLAGLLVVYLVVVACFPAEFRKGESLPVLQGSRARTY
                     GPLLWKSLGAALLMLVLLFAGVPAPLASLVAAALLLVTRRVKPERVFAEVDFSLLVFF
                     AGLFVITKTVEGTGAFRLLVERSMSLVSGGAAATGTGAAAGGSAAVGGAAAGLGAAGG
                     IAGGAALGRFAILTALFSNLVSNVPTVMLFRPLVAWFPDAQKAWLVLAMASTYAGNLT
                     LLGSVANLIVAESAKRRGVDLSFGKYLKAGLPITVLTTAIGTLWLLLV"
     gene            5167..6459
                     /gene="xseA"
                     /locus_tag="PLG14_03545"
     CDS             5167..6459
                     /gene="xseA"
                     /locus_tag="PLG14_03545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOX48236.1"
                     /translation="MYGEERHVYSVSELTGLVKDTLEGGFPAVLVEGEITNCRPASSG
                     HLYFSLKDRQAMLQAVMFRYRTRGLGFEPADGVLVRARGAITVYAARGQYQLLCEGLE
                     RAGQGDLLAMLEERKRRLAAEGLFDESRKRALPRFPRRVAVVTSPTGAAVRDIINVLR
                     RRNSGLALRVLPAAVQGEAAPREIAAQIERANRYDLGDVLIVGRGGGSLEDLLAFSDE
                     AVVRAVAASRIPVISAVGHEIDWALSDFAADLRAPTPSAAAELVSESREALAREVAQL
                     SGELATAMRSRLDRARLLLGRFEPAAVEGRFMKLLHPRIRRLDEARDELAGRMRELAV
                     GARHRLELARGALEASSPEAVLARGYAVVRLASPSAVEAARTAADAGAGVARPGGGSA
                     RPAEGPALRSSEGLKAGDLLDIRFSTGGARARTEEVRG"
     gene            6456..6689
                     /gene="xseB"
                     /locus_tag="PLG14_03550"
     CDS             6456..6689
                     /gene="xseB"
                     /locus_tag="PLG14_03550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOX48237.1"
                     /translation="MKKFEERLERLEELAERIKESDLPLEEAVAVFEEGLKLAKGLEK
                     DLDKIQGRVEILLNEPEGDEEKPELGLFDADED"
     gene            6881..7894
                     /locus_tag="PLG14_03555"
     CDS             6881..7894
                     /locus_tag="PLG14_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOX48238.1"
                     /translation="MTHASPRDRLGSLLSTLLALAGACFLLFAAIPASAEETIRDRAS
                     YAAVKELVVDGGPVGIEARSGAAGSALLVERRDFPPDYRLLADRRGDRLELRVEAPAF
                     QLGFGFAARRPRIILSIPRGALVELGSSSGDISLRGHEALRAVLRSSSGDIEVSDSSA
                     GLELESSSGSIAAARCSGAIAARSSSGGIELEACRGAKDLLTSSGDIALRGSAGDARA
                     RTSSGSFSAFDVSGDLFVETTSGSVSLEGTKGKLSALTGSGDVSGRGVRLTGDSTFGT
                     SSGEVSLELENAASELRFELSSSSGSLAAAGSRGEKRLVAGSGRILVKGSSSSGDQGY
                     STR"
     gene            complement(7973..8371)
                     /locus_tag="PLG14_03560"
     CDS             complement(7973..8371)
                     /locus_tag="PLG14_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOX48239.1"
                     /translation="MSATGVSIASIVLREGLVLVARRERSGPLGGLWEFPGGKVEEGE
                     SEAEALEREFLEEFGAKAKAERRLGETTFMHKGKDRRLVAWLASLPEGERLELREHLE
                     CRWLPPAEISDLDLADSDRKLLPIVEALGP"
     gene            complement(8368..8892)
                     /locus_tag="PLG14_03565"
     CDS             complement(8368..8892)
                     /locus_tag="PLG14_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48240.1"
                     /translation="MIQFYFLSVLFNLVGGYALVEDSARNRHASLEGLRTFLRDPTVR
                     LVIGILSAVVGAFKLLTVVRGDIPVVGDFLPAVAGIAVGATLLLDIYRSPSGDAEGAA
                     SKADGASEGAADGAAAQAGRAESSKAGGSEAKGKARGPGRLESLLREHEEAVGMAGMI
                     AALVHFLFPAVLFL"
     gene            complement(9046..9600)
                     /locus_tag="PLG14_03570"
     CDS             complement(9046..9600)
                     /locus_tag="PLG14_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48241.1"
                     /translation="MSDKDLRGGERPGLGGESPGLRYSRERRLERASPEVRWLNSRYG
                     AKKPGLLKSLFATRASSLLFLTILGLVVAFLLVPLFEGVSKKGGRIGEARFSASALYF
                     EDRVLVAVSRTGGPESAGDDESLVVLAEAEGGAGPRRFEFPFGARVSGDYRLALDSPG
                     RKPKRVALRLSLGGASLDLVLPVD"
     gene            complement(9597..11195)
                     /locus_tag="PLG14_03575"
     CDS             complement(9597..11195)
                     /locus_tag="PLG14_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOX48242.1"
                     /translation="MRPLVASAEALRLDAAVQEAWALQPLVLMENAAARLWLALEPRL
                     GEAGRASPLVALAGGGNNGGDALALLRQARFAGRTDLAAIVAAERMGELAALQRRSLE
                     AAGIAVLSWTEEGDRARGLVGRAGLLLDGLCGAGLKGGLRGPAAGLAAFAAASGRPVA
                     AIDLPSGLSDSMEPDFPVLRSAFCLSIEPRKASLYYPGFREAAGEIVAVEGVFPRDAD
                     LEPSAFLLEEGDLAALAPRPDPAAHKGLRGRLAVFAGSPGASGAAIFASKAAQAASAG
                     LVCLYAPSALYGPLSSDASALGGALVREEEAAFGLPGSVGEDGAFDAVLAGPGWGFRS
                     SASEEEAKRSDSERARLLERLLASGLPAVLDAGALRLLPLVAPGGWKPHHALILTPHP
                     GEFAALSGIPAREVLASPEAPLLEAARRYGAVVVLKAAVTWIAAPDGRLAVYDGREPS
                     LACAGSGDVLAGAAAGLLASRAAAAPVGLAASASPVDLAFDAARGAVLAHGLAGRRAR
                     ARLGWYEAGALPGELARLLSEALP"
     gene            complement(11192..12448)
                     /locus_tag="PLG14_03580"
     CDS             complement(11192..12448)
                     /locus_tag="PLG14_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48243.1"
                     /translation="MKRDKTLSARPARLGAALFALLSAAALFSCGGRSSVAVGREQLF
                     TLGYGPAEDQVDLFQLENSAGPLTTRIAMREGIFYVANGNGAKVVRFSSFGDVLSMLY
                     NPERNPEPLLLKPSAGGEAAGRRAFQYPFRAVGEVAVDSRQTVYVEDRLPPERRVFDK
                     EANSMLDYVVLRFDKDGQFLDYLGQEGVGGTPFPFIVGVWVTAGDDCVVVSVTQSAWL
                     VHWFDPKGLVRHALKIRRDSLPQPAKDAGLIASLDRVVPDSAGKNLILKVDYYREGID
                     PSTKSSSGIEYAGSWAFRMDPSSAAISDRWEIPAETKTAKQGPDGSQARYVRIPEFLG
                     VSGDRLFFITADDDGRSFLSIYDTGARDMKRYSIEIASDELYYSTLFLSPEGILCALL
                     GTKYEARVVWWRFDKLLGAPAKSGEK"
     gene            12656..>12947
                     /gene="secA"
                     /locus_tag="PLG14_03585"
     CDS             12656..>12947
                     /gene="secA"
                     /locus_tag="PLG14_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697621.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOX48244.1"
                     /translation="MSTNILAALFGTKKERDIKGLLPILHSVNEKESWALGLPSSSFP
                     AETDRLRARYKAGETLDQLLPEAFALFREAARRVLGERPFDTQIMGGIVLHQG"
BASE COUNT         1799 a         4470 c         4780 g         1898 t
ORIGIN      
        1 cgatgcagcg cttgaggtag agctcggtgg cgatgcggag gtagagctgg aggttgaggg
       61 cgttggactt cgtggtgaag gggcgggccg cggcgccgga ggcgatcgtc gagaggatgg
      121 gggtctccac ctcgatgaac tcgtggctgt ccaggtactc gcggatggag gcgatgatct
      181 tggtgcgctt gcggaaggtg tccatcgtct cgcggttcat cacgaggtcg aggtagcgcc
      241 ggcggtagcg tacctccgtg tccttgaggc cgtggaactt ctcgggcagg ggctcgaggg
      301 ccttggtgag gagggtgacg gaggcggcct ccacggagat ctcgccgcgg cgggtcttga
      361 agaccttgcc cttcacgccg atgatgtcgc ccatgtccag ggtcttgagc tcggcgtagg
      421 gctccgcgcc caggtggtcc gcgttgaggt agatctggat gcggccgtcg cggtcctgga
      481 ggtcgacgaa ggaggccttg ccgatgtcgc ggaaggacat gatgcggccg gcgaccgaga
      541 cctccgcgcc gtcgaggctc tcgaagcctt ccttgatctg gagcgagtgg tgggtctggt
      601 cgtacttgac gacggcgaag ggatccttgc ccgcggcctg gagcttggcg agtttctcgc
      661 ggcggatccg cgcctgctcg gagagggcgc gctcggcggc ggcgccgtcc tcggcggtct
      721 ccgcggcctc gtgggcggcg gactgggtca cggcggcctt ggtctcggcc cgctcttcct
      781 tcttggtcct gtcatcggcc atggtatccc tcggtggctt cgtagaattg ggggcattat
      841 agcggaaggg gcggttttgc ggcaagcgcg gaggccgggg cccgggcggc gccgcggcac
      901 gagcagcgcc gcgacctgac ccggccgctc gcgggagccg ggggcggcgg agcggaccgg
      961 ccgtacgcgg gagccgggac agcgcgtccc aggcccgggg gcctcagggc cgcgcccggg
     1021 cctgagaggc ctactcgaag aagctcgtga gcttccggta gagggcgctc accttctcct
     1081 tctcctcggg gctggcgcct tcgcccaggg cgtcggccag ggcgtccagg gaggcgacgc
     1141 tctcgttcag ggccgtgtgg aagccccgcg agcagcggaa ccagtagttg ccctccgagt
     1201 cggtgaagag ggccacgaag ccgagctccg ccgactggtc cttggcgcgg gccacctgga
     1261 ggacgtggtc cagggaggca aggagctcgt ggaggccctc gcccgagtcg aagttcttct
     1321 tccgcggcca gtcgcgcgcg aggtcgcgct cggggtccag gctgggcgcg aggcggagga
     1381 tgaggtcgag cttctcgccc tcgaggagct tccggtgccc gacgaggaca acgcccttca
     1441 tgcccttgta gacggccagg gactctcggt cgagctcctc gcgaaccttc gcgagcttct
     1501 gccactcgag ggagacgacc ttcttctttt tcttgtcgac ggccaggtcg aagacctcgc
     1561 tgccgatctt gatcttgttc gtgaagtccc ggactggcct ttcggcgccg gccgcgggtt
     1621 cggcgagggc ctcgtcctcc tcgcggcggc cgtggcggcc gctccgcctg tcggcctcac
     1681 cgcgctccag gcgatggcgc tcgccgggcc gctcgcccgg ccggccgcgc tcggcgtagc
     1741 cctcctcgcg ggagcgcccc gcggccgcgg agacgtagcc gccgcgcagg aggccctcca
     1801 gccgggggga gatccgggag agccaggcct tctccagggg ggagacggac atcgcgtaca
     1861 tgcgcgtggt gcgcacgatc tcgcccgcga cgatgtagtt cgggttctcc ttgtacatca
     1921 ccgagccggg atggatctgg atcttctccg ccgtgaggct gcggaagagg ccccggtcct
     1981 ggcgggcgca gacgaactgg atgaggccgc ggctcacggc gcagaggtag tcctcgcagc
     2041 ggccgccccc gacgatggga acgcccaggc cggagacgat gagctcgagc tgctccttta
     2101 tgcggaagat ctccagcatg gtgcgctcgt ccaggtagct ccgctcgcag aacttcgcct
     2161 tgtccctggc ctcgcggaag gccgcgaaga tcttgaggta ggagacgaag tcgcccgagg
     2221 ggtcgcggaa ggcgtggtgg gcccgccggg cctccatctc ctcgccgggg ggcaggacgt
     2281 aggggctcgt ggtcgagagg aagctcgtgc cgaccagggc ctcctccacg acgtcgggat
     2341 actggaggac ggcctccacg atgatgcggc tgtgccgggg gaggaggggg aaggcgcaca
     2401 tcatctcgcc gacgcgggtg aggccgcgct ccggcgtgag ggcgtccagg agggccaggg
     2461 cctcctcagc gcccaggatg ccggacttcg gaggctggga gatgaactcg aagcgctcga
     2521 actcggagat gccgagctcc gccatgcgca ggacgacctc cgtgaggtcg gtgcggtaga
     2581 tctcctccgt cgtgaagagg gggcggccct cgaagtcgct ccggccgtag aggcggtagc
     2641 agacgccggg cctggtgcgg cccgcccggc ccttgcgctg gttgcaggag gccttggaga
     2701 tgggcgtctc gatgaggctg gaggtgaagc tcttagggtt gtagtagttg agcttggcca
     2761 gccccgagtc gatgacgctc gtgatgccgt ctatcgtgag ggaggtctcc gcgatgttcg
     2821 tggagatgac gaccttcgtc ttgcccgggg gcgggacctc gaagacgcgc tcctgctcgt
     2881 ccttgccgag gcgggcgtag agggggatga cgtggaggcg gtcgccgacg gggccgaagg
     2941 ccaggcgctt catgcagtcc ttgatggcct tctctcccgg gaggaagacc aggatgtcgc
     3001 cctcgcgccg ctcgcccacg atgcggccga cgatctcctc gatcttggcc tggagggcgt
     3061 cctccgcggc gaagcggctg ccctcgtcct cgggggccgc ctgggcgggg tcgtagacga
     3121 cggtgacggg gtacatcggg gtctcgatct tcacgatggg gcactggccg aagtactcgg
     3181 agaagacctc cgcgttgatc gtcgccgagg agacgatgac cttgaactcg gggcgggcct
     3241 cgatgacccg cttcacgagg cccaggatga agtcgatgtt gaggctccgc tcgtgggcct
     3301 cgtcgaccat gaggacggag tacttggaga gggcggggtc gagcttcatc tcctggagga
     3361 ggatgccgtc cgtcatgatc ttgatggcgg tcgagtcgtt cgtcttgtcc tcgaagcgca
     3421 tcttgtagcc gacgacgccg ggcacggtcg tgccgatctg cctcgcgatg aagtcggaga
     3481 ccgagagggc ggcgatgcgc cggggctggg tgacgccgat gaggccgaac ttcgagtagc
     3541 ccgcctcgtg gaggatgatc ggcagctggg tggtcttgcc ggagcccgtg gggctctcta
     3601 cgacgacgac ctggtggtcc ttgaggacct cgaggatgcg ctccttctgc tggtagaccg
     3661 ggagctcgtg tggattcatg ggaaagattg tagcgttttc cgccgccttg cgccaggagg
     3721 ggcgcggccg ctatactcgc ggcaaaacca tgagagagct cttcaccgac gtcgccgtcc
     3781 tcgcggccct tgccgcaata gccgccggcc gcctgccccg cctccgcatg aaccgcgcca
     3841 ccatcgccct ggccgccgcc gccctcctcg tcgccgcggg ctccataggc cttgaggagg
     3901 ccttcgccgc gatcgacctg ggcaccgtct gcctcctcct ttcgatgatg gtcatcgtcg
     3961 cgaacctccg gctctcgggc ttcttcgagg cggcgggagc gaagctcctg gccgcggccc
     4021 ggggcccgcg gcagctcctg gccctggtcg tcctggcctc gggcttcctc gcggccctct
     4081 tcctcaacga cacggtctgc ctcatgctca cccccctcgt cgtcgagctc gccctgcgct
     4141 cgaggaggga tcccctgccc tacctggtcg ccctggcgac ggcggccaac gtgggttcgg
     4201 cggcgacgat cgtcggcaat ccgcagaaca tgctcatcgg ggcctcgagc gggattccct
     4261 tcggccgctt cctcctcatg ctcggtccgc cctccctcgc cggcctcctg gtcgtctacc
     4321 ttgtcgtcgt cgcctgcttc cccgcggaat tccggaaggg cgagtccctg cccgtcctcc
     4381 agggctcgcg ggcgcggacc tacggccccc tcctctggaa gagcctgggc gcggccctcc
     4441 tcatgctcgt actccttttc gcgggggttc cggcccccct cgcctccctg gtcgccgccg
     4501 ccctcctcct ggtcacgcgg cgggtcaagc cggagcgggt cttcgcggag gtggacttct
     4561 ccctcctcgt cttcttcgcg gggctcttcg tcatcacgaa gacggtcgaa ggtacggggg
     4621 ccttccgcct cctggtggag cgctcgatgt ccctggtctc gggcggcgcg gccgctactg
     4681 gaaccggcgc cgccgccggg ggctccgcgg cggtgggcgg ggccgcggcg ggcctcggcg
     4741 cggccggagg catcgccggg ggcgcagccc tgggccgctt cgccatcctc accgcgctct
     4801 tctcgaacct ggtctccaac gtgcccacgg tgatgctctt caggcctctc gtcgcctggt
     4861 tcccggacgc acagaaggcc tggctcgtcc tcgcgatggc gagcacctac gcgggcaacc
     4921 tcaccctctt aggctccgtc gcgaacctca tcgtcgccga aagcgccaag cgccgcggcg
     4981 tcgatctctc cttcgggaag tacctgaagg ccggcctccc gatcacggtc ctcacgacgg
     5041 ctatcgggac gctctggctc ctcctcgtct agggcaggcc gccggggagc ggccaccccg
     5101 cgcgcgagcg cttccgtcaa ggcctccccg ttgcgccccc cgcgccggcg cgctatcatg
     5161 cgggcgatgt acggcgaaga gcgacacgtc tactcggtct ccgagctcac cggcctcgtg
     5221 aaggacacgc tggagggcgg cttccccgcc gtcctggtcg agggcgagat cacgaactgc
     5281 cggcccgcga gctcaggcca cctctacttc agcctcaagg accgccaggc catgctgcag
     5341 gcggtgatgt tccgctaccg gacgaggggc ctgggcttcg agcccgccga cggcgtcctc
     5401 gtgcgggcga ggggcgccat caccgtatac gccgcgcgcg gccagtacca gctcctctgc
     5461 gagggactgg agcgcgccgg acagggcgac ctcctggcca tgctggagga gaggaagcgc
     5521 cgcctggcgg ccgagggcct cttcgacgag tcgaggaagc gcgccctgcc ccgcttcccg
     5581 cggcgggtcg ccgtcgtcac ctcgcccacg ggcgccgcgg tgcgcgacat catcaacgtc
     5641 ctaaggcggc gcaactccgg gctcgcgctc cgcgtcctgc ccgcggccgt ccagggcgag
     5701 gccgcgccgc gcgagatcgc cgcgcagatc gagcgggcca accgctacga cctgggcgac
     5761 gtcctcatcg tgggccgcgg cggcggctcc ctggaggacc tcctcgcctt ctccgacgag
     5821 gccgtcgtgc gcgccgtcgc cgcctcccgg atccccgtga tcagcgccgt cggccacgag
     5881 atagactggg ccctctccga cttcgccgcc gacctgcgcg cgcccacgcc cagcgccgcc
     5941 gccgagctcg tctccgagag ccgcgaggcc ctggcccgcg aggtggccca gctctccggc
     6001 gagctcgcga cggccatgcg ctcgcgcctg gacagggccc gcctcctcct gggccgcttc
     6061 gagcccgccg ccgtggaggg ccgcttcatg aagctcctcc acccgcggat ccgccggctg
     6121 gacgaggcga gggacgagct cgcggggagg atgcgcgagc tcgccgtcgg cgccaggcac
     6181 cgcctggagc tcgcgcgcgg agccctggag gcctcgagcc ccgaggccgt cctggcgcgc
     6241 ggctacgcgg tggtgcggct ggcctctccc tcggccgtcg aggccgcgcg gaccgccgcc
     6301 gacgcggggg ccggagtagc gcggcccggt ggcggatcgg cgcgtcccgc ggaggggccg
     6361 gccctgcgga gctcggaggg cctcaaggcg ggcgacctcc tggatatacg attctctaca
     6421 ggcggggcca gggcccggac ggaagaggtg cgcggatgaa gaagttcgaa gagaggctcg
     6481 agcggctcga ggagctggcc gagcgcatca aggagagcga ccttcccctc gaggaggcgg
     6541 tggccgtctt cgaggagggc ctcaagctcg cgaagggcct ggagaaggac ctggacaaga
     6601 tccagggcag ggtggagata ctgctcaacg aacccgaggg cgacgaggag aagcccgagc
     6661 tcggcctctt cgacgccgac gaggactgag gcgcgcgtat tttccgctcc ggcgccttga
     6721 ttccgcgccc ccctcgcgat atttttcccc cgtaacgccg gcgtcgccgg cttccgcgcc
     6781 cgcgcgtcgg ggcggagtga atggaggcgg ccccgcaggg gcgcctaccg ccgctgcccg
     6841 gcatgcgcgt cacgagagcg cgggccggca aggagagccc atgacccacg caagcccgag
     6901 ggaccgcctc ggctccctcc tatccaccct tctcgccctc gcgggcgcct gcttcctcct
     6961 cttcgccgcg atccccgcct cggccgagga gacgatacgc gaccgcgcct cctacgccgc
     7021 ggtgaaggag ctcgtcgtgg acggaggccc cgtcggcatc gaggcgcggt ccggcgccgc
     7081 gggctcggcc ctcctcgtcg agcgccgcga ctttccgccc gactacaggc tcctcgccga
     7141 caggcgcggg gacaggctcg agctccgcgt cgaggcgccg gccttccagc tcggcttcgg
     7201 cttcgccgcg cgtaggcctc gcatcatcct ttcgattccg cgcggcgccc tggtcgagct
     7261 cggctcctcc tcgggcgaca tctccctgcg gggccacgag gccctccgcg ccgtcctccg
     7321 ctcgagctcg ggcgacatag aagtctccga cagctccgcc gggctcgagc tcgagtccag
     7381 ctcgggctcc atcgccgccg cgcgctgctc gggcgcgatc gccgcgcgct cgagctcggg
     7441 cggcatcgag ctcgaggcct gcaggggagc caaggacctc cttacgagct ccggggacat
     7501 agccttgcgc ggctcggcgg gcgacgcgag ggcgaggacc tcctccggga gcttctccgc
     7561 cttcgacgtc tcgggcgacc tctttgtgga gaccacctcg ggaagcgtca gcctggaggg
     7621 caccaagggc aagctgtccg ccctgacggg ctcgggcgac gtctcgggca ggggagtccg
     7681 cctcacgggc gactccacct tcggcacgag ctcgggcgag gtgagcctgg agctcgagaa
     7741 cgcggcctcg gagctgcgct tcgagctttc gagctcgagc ggctccctgg cggcggccgg
     7801 ctcccgcggc gagaaaaggc tcgtcgccgg ttcgggacgc atcctcgtga agggcagctc
     7861 gagctcgggc gaccagggct actcgacgcg ttagtccgga tagacaggaa ggacgcgaaa
     7921 aggggctgcc ctcttggaca gccccttgcc gtgtactgcg ccgcccaagg agctacggcc
     7981 ccaaggcctc cacgatgggg aggagcttcc ggtccgaatc ggccaggtcg aggtcggaga
     8041 tctccgcagg ggggagccag cggcactcca ggtgctcgcg gagctcgagg cgctcgccct
     8101 cggggaggct cgcgagccaa gcgacgaggc gcctgtcctt gcccttgtgc atgaaggtgg
     8161 tctcgcccag gcgcctctcg gccttggcct tggcgccgaa ctcctccagg aactcgcgct
     8221 ccagggcctc cgcctcgctc tcgccctcct ccaccttgcc gcccgggaat tcccagaggc
     8281 cgcccagggg gcccgagcgc tcgcggcgcg cgacgaggac caggccctcg cggaggacga
     8341 tcgaggcgat cgagacgccg gtcgcgctca caggaagagc accgccggga agaggaagtg
     8401 gacgagggcg gcgatcatgc ccgccatgcc cacggcctcc tcgtgctcgc ggaggaggct
     8461 ctcgagcctg cccggccccc tcgccttgcc cttggcctcg gagccgccgg ccttggagga
     8521 ctcggcgcgc ccggcctggg ccgccgcgcc gtcggccgcg ccttccgagg cgccatcggc
     8581 cttggaggcg gcgccttccg cgtcgcccga gggggagcga tagatgtcga ggaggagggt
     8641 ggcgccgacg gcgatgcccg ccacggcggg caggaagtcg ccgacgacgg ggatgtcgcc
     8701 gcgcacgacg gtgaggagct tgaaggcgcc gacgacggcg gagaggatgc cgatcacgag
     8761 gcgcacggtc gggtcgcgga ggaaggtgcg caggccctcc agggaggcat gcctgttcct
     8821 cgcggaatcc tcgacaaggg cgtagccgcc cacgaggttg aaaagcaccg acaggaaata
     8881 gaactgtatc atcgcagact ccgaggcgcg ccccgcgggg gagagagccc gcggttacag
     8941 tataggaaag cccggctggg gtcaaggcgg ccccttgtcc gcggggccgt ggggacgcgg
     9001 cgccgcgccc gccgattcct tgtccgcggg gccgcccggc cgcggctagt cgaccggaag
     9061 gacgaggtcg aggctcgctc cgcccaggga aagccgcagg gccacccgct tgggcttcct
     9121 gcccggcgag tcgagggcga ggcgatagtc gcccgagacc cgcgcgccga aggggaattc
     9181 gaagcgcctg ggccccgcgc ctccctcggc ctccgcgagg acgacgaggc tctcgtcgtc
     9241 gcccgccgac tctggcccgc ccgtgcgcga gaccgcgacc aggaccctgt cctcgaagta
     9301 gagggccgag gcggagaagc gggcctcgcc gatgcgtccg cctttcttgg acacgccttc
     9361 gaagaggggc acgaggagga aggcgacgac caggccgagg atggtcagga agaggaggct
     9421 cgaggccctg gtcgcgaaca gcgacttgag gagtcccggc ttcttggccc cgtagcgcga
     9481 gttgagccaa cgcacctcgg gggaggcgcg ctcgagccgt cgctcgcgcg agtagcgcag
     9541 gcccgggctc tccccgccca gtcccggcct ctccccgccc ctcagatcct tgtcgctcat
     9601 ggcagcgcct ccgataggag cctcgcgagc tcgccgggca gtgcgccggc ctcgtaccag
     9661 ccaaggcggg cccgcgcgcg ccgacccgcg aggccgtggg cgaggacggc gccccgggcc
     9721 gcgtcgaagg caaggtcgac cggactcgcg gaggccgcga ggccgactgg ggccgccgcg
     9781 gcgcgcgagg cgaggaggcc ggccgcggcg cccgcgagga catcgcccga gcccgcgcag
     9841 gcgagggagg gctcgcggcc gtcgtagacg gccagcctgc cgtcgggagc cgcgatccag
     9901 gtgaccgccg ccttgaggac gacgacggcc ccgtatcgcc gcgccgcctc gaggagggga
     9961 gcctctggcg aggcgaggac ttcccgcgcc ggtatgccag aaagggcggc gaattccccc
    10021 ggatggggcg tgaggatcag ggcatggtgg ggcttccagc cgccgggcgc gacgagaggc
    10081 aggagcctca aggcgcccgc gtccaggacg gcgggcaggc cggaggcgag gaggcgttcg
    10141 aggagccttg cgcgctccga gtcgctccgc ttggcttcct cctcggaggc cgaagagcgg
    10201 aagccccagc ccggccccgc gaggacggcg tcgaaggcgc catcctcgcc gacagagcca
    10261 ggcaggccga aggccgcctc ctcctccctg acgagggctc cgcccagggc cgaggcgtcg
    10321 gaggagaggg gaccgtagag ggctgagggc gcgtagaggc agacgagacc ggcggaggcc
    10381 gcctgagcgg cctttgaggc gaagatggcc gcccccgagg cccccggcga gcccgcgaag
    10441 acggccagcc ggccgcggag gcccttgtgc gcggcgggat cgggccgcgg cgccagggcg
    10501 gcaaggtcgc cctcctcgag gaggaaggcc gagggctcga ggtccgcgtc ccgggggaag
    10561 accccctcga ccgcgacgat ctcgcccgcg gcctcgcgga agcctggata gtagagggaa
    10621 gccttgcgcg gctcgatgga gaggcagaag gcggacctga ggacgggaaa gtccggctcc
    10681 atggagtcgg agagccccga ggggaggtcg atcgcggcga cgggccggcc cgaggcggcg
    10741 gcgaaggccg cgagcccggc ggcggggccg cggaggccgc ccttgagccc cgcgccgcag
    10801 aggccgtcta ggaggaggcc ggcccggccg acgaggccgc gggcccggtc gccctcctcc
    10861 gtccaggaga ggacggcgat gcccgcggcc tcgaggctcc gccgctggag ggcggcgagc
    10921 tcgcccatcc tctcggcggc gactatcgcg gccaggtcgg tcctgcccgc gaagcgggcc
    10981 tgcctgagga gggccagggc gtcgccgccg ttgttgccgc ctcccgcgag ggctacgagg
    11041 gggctcgccc tccccgcctc gcccaggcgc ggctcgaggg ccagccagag ccgcgcggcc
    11101 gcgttctcca tgaggacgag aggctgaagg gcccaggcct cctggacggc ggcgtccagg
    11161 cgcagggcct cggccgaggc gacgaggggc ctcacttctc tccgctcttg gccggcgcgc
    11221 ccaggagctt gtcgaagcgc caccagacga cgcgggcctc gtacttggtc ccgaggaggg
    11281 cgcagagtat gccctcgggc gagaggaaga gggtcgagta gtagagctcg tcggaggcga
    11341 tctcgatgct gtagcgcttc atgtcccgag cgcccgtgtc gtagatcgag aggaagctcc
    11401 gcccgtcgtc gtcggcggtg atgaagaaga ggcggtcgcc cgagacgccg aggaattccg
    11461 ggatgcgcac gtagcgcgcc tgggagccgt ccggcccctg cttggccgtc ttggtctccg
    11521 cggggatctc ccagcggtcg gagatcgccg cggaggaggg atccatcctg aaggcccagg
    11581 agcccgcgta ctcgatgccg gaggaggact tggtcgaggg gtcgatgccc tcgcggtagt
    11641 agtcgacctt gaggatcagg ttcttgccgg ccgagtcggg gacgacgcgg tccagggagg
    11701 cgatgaggcc cgcgtccttc gcgggctggg gcagagagtc ccgcctgatc ttgagggcgt
    11761 gcctcacgag gcccttcggg tcgaaccagt ggacgagcca ggcgctctgc gtgacggaga
    11821 cgacgacgca gtcgtccccc gccgtcaccc agacgccgac gatgaagggg aagggcgtgc
    11881 cgcccacgcc ctcctggccc agatagtcga ggaactggcc gtccttgtcg aagcgcagga
    11941 cgacgtagtc gagcatgctg ttcgcttcct tgtcgaagac gcggcgctcg gggggcaggc
    12001 ggtcctcgac gtagacggtc tggcgcgagt cgacggccac ctcgcccacc gcgcggaagg
    12061 gatactggaa ggcccgcctg cccgcggcct cgcctccggc ggagggcttg aggaggaggg
    12121 gctcggggtt gcgctcgggg ttgtagagca tggagaggac gtcgccgaag gaggagaagc
    12181 ggacgacctt ggcgccgttg ccgttcgcga cgtagaagat gccctcccgc atggcgatgc
    12241 gggtcgtgag cgggccggcc gagttctcga gctggaagag gtcgacctgg tcctcggcgg
    12301 ggccgtagcc gagggtgaag agctgctccc tcccgacggc gacgctgctc ctgccgccgc
    12361 aggaaaaaag cgccgcggcc gagaggaggg cgaagagggc cgcgccgagc cgggcgggcc
    12421 tagcggagag ggttttgtcg cgcttcatac ggcttaaagt agtacctccc gcggccccaa
    12481 gtgtcaacct tgggccgggc ccggccccgc ccgcggagct ccgcggcgga agggcccgcg
    12541 gcgggccggc gcgcttccat cggcgctggg cgcggccttg ggcgcgccgc gaaaggccct
    12601 ccggagagcc gccggagctt gacccctccg attcaatgaa ggtacattaa cgggcatgag
    12661 cacgaacatc ctcgccgcgc ttttcgggac gaagaaggag cgcgacataa agggcctcct
    12721 ccccatcctc cactcggtca acgagaagga gtcctgggcc ctcggcctcc cctcctcctc
    12781 cttccccgcg gagaccgacc ggctccgcgc ccgctacaag gccggcgaga ccctcgacca
    12841 gctccttccc gaggctttcg ccctcttccg cgaggcggcc aggcgggtcc tgggcgagcg
    12901 gcccttcgac acccagatca tgggcggcat cgtcctccac cagggca
//
LOCUS       DAOOSJ010000043        12944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3235_length_12944_cov_6.486694, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000043 DAOOSJ010000000
VERSION     DAOOSJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_12944_cov_6.486694"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLG14_03590"
     CDS             <1..781
                     /locus_tag="PLG14_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOX48245.1"
                     /translation="VAGGRRISVKPTLLVTLVGISPDVRKTPSTDFLEAGDLLYLVGS
                     GGAWELGCSILERVLAARAAEGLRGEAAREARGSFSLGPAPRARPLEHRGAYRALAKA
                     ISGRACRSCHDLSDGGLAAALAESCLGGRLGAELRLDGLAEELAARLAAAAEEAARAG
                     SAAPGGGAGGSAAGGAAVSAASPGGIAPRLLFSEAPGRLLVSVRPEDRERFERSMAGE
                     ACRLVGRTCAEPRIGALLQGAAVLDLGLDEALAAFKTPISR"
     gene            791..1594
                     /locus_tag="PLG14_03595"
     CDS             791..1594
                     /locus_tag="PLG14_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOX48246.1"
                     /translation="MEPKAIIVTGNGINSDAELAEAFRLAGAAPERVHLSDLVAEPER
                     LLRARLLGFPGGFSYGDHLGSGQVLALLCRRALKPALERFVAEGGLVIGVCNGFQALA
                     KMGMLPNRGPAAGLQPWEREVTLVHNPDAHYVDKWVPLRFEPASRCAWTRGLPPRLLP
                     IRHGEGRFVARDAATLAALEDEGLVALRYGLTGGRPAPGAAPSPDNPNGSEADIAGIC
                     DSTGRVFGLMPHPEAFLVPENHPDRRRGRRDPVGLDIFENGVRAAREAT"
     gene            1693..2751
                     /gene="dgcA"
                     /locus_tag="PLG14_03600"
     CDS             1693..2751
                     /gene="dgcA"
                     /locus_tag="PLG14_03600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HOX48247.1"
                     /translation="MSDIPEPGAALADSERDDYEKQIFDLRQLLEVSKSLNSTLDYNI
                     LIDSILFTIMGQMKVIKAGLFAKKGIDTPYFSLHRNYKGFDVDHRVNYSISEDHPAIK
                     LFTRQYGCYTLEDIKRRFGSLQGLEGLASLEPSLIVPLKAKGVINGILVLGERIDDSG
                     FDAYEREYILTLAALAAVAINNAFLFEMTTTDMMTKLKMKHYFYTVLIERMEQAALAG
                     KPLSIVMLDIDFFKKFNDTHGHLCGDAVLKQVARILQSCVRAVDLAARYGGEEFCVLL
                     PDADRERAKAVAERIRQSVAGTPTEYEGLSLLVTVSVGVAQYDPARDVSAKSLIDRAD
                     RALYFSKQNGRDRVTAAE"
     gene            2945..3199
                     /locus_tag="PLG14_03605"
     CDS             2945..3199
                     /locus_tag="PLG14_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX48248.1"
                     /translation="MSLQRAGAPLRHDCGGKALCGTCRVRVLSGAEGLSPMGERERLR
                     LEAVGAPLDGSVRLACQARASRDAEIEALLGPAEGKGEGR"
     gene            3196..4929
                     /locus_tag="PLG14_03610"
     CDS             3196..4929
                     /locus_tag="PLG14_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX48249.1"
                     /translation="MRHWWEDAVFYQIYPRSFADSGGDGVGDLPGIRSRLGYLAELGV
                     DALWISPFFPSPMKDFGYDVSDYRGVDPLFGTLDDFKALSAEAGRLGLRIVLDLVANH
                     SSDEHPWFKAARASREAKEHGYYLWKPLEGKVPNNWKSLFELRSAWYPNPQTGERYLA
                     TFTRSQPEFDWRNPELRREMYDVMRYWYGLGVDGFRLDVATAYFKDAEFRSNPFSWKA
                     NPDLFQRHVYDRNRPEVHEVFREMRKVADEAGERVLIGETHGQEAELAASCHGEAGDG
                     LHMAFNFDFLNQPWSAAAFRRSAEAWYAALPEGAWPNFTLSNHDRPRAAFRFRSRDPR
                     VTEGRARVAAAMLLTLRGTPFVYYGEELAMGCVRLPRASLRDPLGIRTWPLGFLGRDP
                     ERTPMQWGSGPGAGFTKGEPWLPLNPDWRERNVEAQAADPASTLAWYKSLLGLRRSRE
                     ALRSGSISFLDAGRGSRASEVLAYERRLGVSAAGQAGPGGAAGAGSGAAEGSAAGPAE
                     GPVAAAAGSGGRLFVLLNFSSRPRKAGLPGRARVLLGGAPLDGSPRTAGEVLGPDLEL
                     RGLEALVLEAL"
     gene            complement(4926..5924)
                     /gene="holA"
                     /locus_tag="PLG14_03615"
     CDS             complement(4926..5924)
                     /gene="holA"
                     /locus_tag="PLG14_03615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOX48250.1"
                     /translation="MKIEPCYLLAGPETGKRSAFVAELREAIARADGAPAEEHRLYST
                     DAGVGELLALLRNGSLFASRRLVEYRGAEAIKGKEDTGALAAYLASPAPDAVLLLVTD
                     AFYAEKAVEEAVGKERKKTFYELFESEKPRWVERRLRELGLGIDEEGVEALLELVEND
                     TSALESACSRLSVLFPQGSVLGEAEIEAAVARNRQEDAFSLFARMAEDEPAWALECLE
                     TVLADRQGGAVQILSALVWSFRRLLRLHLLLEGGEGFETACLKSGIRAKSLQGLHRKA
                     LARYERRDCERIIGLAAEIDARARAAGSVLERPLLQLFVYGAMVKKGRLELSVPSA"
     gene            complement(5926..6525)
                     /gene="lexA"
                     /locus_tag="PLG14_03620"
     CDS             complement(5926..6525)
                     /gene="lexA"
                     /locus_tag="PLG14_03620"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOX48251.1"
                     /translation="MKSLTLRQQEVLDFIADFIEEHAYPPTIREIADSFSISVKGAYD
                     HVKALEKKGKLRLGENRSRALEIIRPERAERSVVQLPLLGVVAAGKPVFAEENFSGSV
                     PVPADMVRSSSCFALTVRGDSMKDAGIFNGDIAVIEERQSAENGEIVVAMIDDAVTLK
                     RFYRENNRVKLVAENPAYSPIFTQDVRIVGRLRGLIRKY"
     gene            complement(6522..7919)
                     /locus_tag="PLG14_03625"
     CDS             complement(6522..7919)
                     /locus_tag="PLG14_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX48252.1"
                     /translation="MASILIIDDEPGIRSAVRGILEDERHKAFAAEDALVGLEILERE
                     RVDLVLLDVWLPRMGGLEALAEFKRRRPELEVIVISGHGTIDMAVRAIKLGAFDFIEK
                     PLSIDRLLTGVRNALEITKLRRENRSLKSAGRAACEELVGGSAAMATVRSLAEQAAAS
                     DARVLISGENGTGKELVARLIHAKSGRAEAPFVAVNCAAIPEGLLESELFGHEKGAFT
                     DAVSRRKGRFEAADGGTLFLDEVADLSPSAQAKLLRVLQEMRFERLGSEETVSVDVRV
                     LSASNKDLREEVAAGRFREDLYFRLAVVPIRMPALRERLEDLPALVGRFLELSGGGRS
                     LAGDALAALAARPWPGNVRELRNAVERLRVLSDEEPIGAAEVERVLGEAGARTGGAGG
                     NGEGKGGGAKETLVAPRFLKLGLQPAKELFEREYLVHKLRECGYNVSRTAEAIGVYPS
                     GLHAKLKKLGIEPEK"
     gene            complement(7984..9921)
                     /locus_tag="PLG14_03630"
     CDS             complement(7984..9921)
                     /locus_tag="PLG14_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48253.1"
                     /translation="MSGGGAGGPGPAGTDPATADAASGAAPAATADAASGAAPSPVAA
                     AAGAPGSGARRPAPDSPKRSRGLTDLLALLVVYAAVALGAYVYARGVLSEAPSFPGAE
                     ERLPLLLLGVLPAGFLLVFAYRLRRLAAELRGRRYGSRLKARLVALFLVAVAASSIPQ
                     GVFLLRLARRAQSQAASADIRRGLEGGLRLVLAAYDEDLRRLEYAARNDLPALAPGRL
                     PERPDRLLEALRAREPRMEAVELFERGASAAFAGDRAARLAEVPGAAASPAAAAPGPL
                     PASQEGGVGRLRYLVPWGPSGAVVLTLRLPEGFDRTAGELARGKSQAELMAAFSPRWG
                     RLLLLLYAYLVLPLLLLAALFGVAAAELVVEPLASLEEATRRVASGELSLRLLAKPGD
                     ETGRLVASFNAMLGGIERYREGDLRKGKIDAWKDIAQRLAHELKNPLTPIRLAAERLL
                     RLAGKDPERALELIEPSALAIVAEVESMDALLSDFRSFAALPEPVRGWTELRGVVEEA
                     VALYAASYPGLRFELAGLPEGILLLADRAQLKRALSNLLSNAVDAMEGSGRVEFAAEL
                     VKTAESRYCRLLVRDDGRGMPAEVLGRIFAPYFTTKPAGTGLGLSIVERIVADHGGSI
                     RAESEEGVGTSFYIDLPAAGP"
     gene            complement(9918..10184)
                     /locus_tag="PLG14_03635"
     CDS             complement(9918..10184)
                     /locus_tag="PLG14_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOX48254.1"
                     /translation="MTERNVTIKNRAGIHARPAALIVQTSSKFGSKIWLEKGSDKINA
                     KSIMGIITLGASFGTPIRILADGADEEEAAAAIERLFESRFEEE"
     gene            complement(10251..11240)
                     /gene="hprK"
                     /locus_tag="PLG14_03640"
     CDS             complement(10251..11240)
                     /gene="hprK"
                     /locus_tag="PLG14_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOX48255.1"
                     /translation="MEEQRFTVLDLLDLDLKEQNALDLRCICGRKGLSREITVPDLNR
                     PGLALSGFFESFAFQRLQVFGRGENAYLKKIEEEDKTESFEKLFSYPIPACIFTHNLA
                     PGRNFFRLAEEGGCPVLQTGLSSSELTSRILRVLSDIFAPKATLHGVLVEVYGIGILL
                     AGDSGVGKSETALELVERGHRLVCDDVVEIRCVNGSILMGQGANKVIGHHMEIRGLGI
                     INVTHLFGVGAIRDKKQIQLVCNLEEWDADKVYDRIGTEEKTTDILGVRVPQLVIPVK
                     PGRNVPIIIETAAMNERLKKMGYHSAKEFNQNILRWLESENARAAYFNRDDYL"
     gene            complement(11400..11690)
                     /gene="raiA"
                     /locus_tag="PLG14_03645"
     CDS             complement(11400..11690)
                     /gene="raiA"
                     /locus_tag="PLG14_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOX48256.1"
                     /translation="MTVDVRSVHFNLSDTSRDYLDKKLERIGYADDKVVDLIFVFTKE
                     KDFKLEATVNFRWGTQAHVEERDADVNPGIDKLIDRLEQKITKEKEKVQEKR"
     gene            complement(11830..>12944)
                     /gene="rpoN"
                     /locus_tag="PLG14_03650"
     CDS             complement(11830..>12944)
                     /gene="rpoN"
                     /locus_tag="PLG14_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOX48257.1"
                     /translation="GLDDDSKRMFIEGAIARPETLQEHLLWQLRLQPLDEDSRAIGEA
                     LIQNLDDDGFHKEDPYSLKPGVPRGKVDEALALVRGLEPAGTCTADYRESLRVQAALA
                     PAAPAAALAILDGHVELLEKGRHAEIQKRLKLGEAELALALAFIKELSPFPGRAYSKE
                     EPRYVLPDLLVKLKDGDFVIVLNDEEIPVLGIDPFFDSLADKKGGDKATGAYVRDNVK
                     RAKFFIHSIRQRNQTLLKVARAVVEFQRAFFAKGPKHLAPLTLKDVAAEVGVHETTVS
                     RIAHSKYLQTDFGIFELRYFFTNSISGAGSSGSRFSKEGVKQVIKEIIEGEDSALSDR
                     DIAEHLARRGITLARRTVAKYRGELDLDSSFGRKRL"
BASE COUNT         1768 a         4538 c         4710 g         1928 t
ORIGIN      
        1 ggtggcgggc gggcgccgga tctcggtgaa gccgaccctc ctcgtcaccc tggtcgggat
       61 ctcgcccgac gtcaggaaga cgccctccac cgatttcctt gaggcggggg acctcctcta
      121 cctcgtgggt tcgggcgggg cctgggagct cggctgctcc atactggagc gggtcctcgc
      181 ggcccgcgcc gccgaaggcc tgcggggcga ggccgcccgc gaggcgcgcg gctccttctc
      241 cctgggcccg gctccgcggg cgaggcccct cgagcaccgc ggcgcctacc gcgccctcgc
      301 gaaggcgatc agcggccgcg cctgccgctc ctgccacgac ctctccgacg gcggcctcgc
      361 ggccgccctg gcggagtcct gcctgggcgg ccgcctgggc gccgagctca ggctcgacgg
      421 cctggcggag gagctcgcgg cgcggctcgc cgcggccgcc gaggaggccg cccgcgccgg
      481 ctcggcagcg ccaggcggcg gcgcgggcgg gagcgcggcc ggcggcgcgg cagtgagcgc
      541 agcgtctccc ggcggaatcg ccccgcgcct cctcttttcg gaagcgccgg gccggctcct
      601 cgtctccgtg cgtcccgagg acagggagcg cttcgagcgg agcatggccg gcgaggcctg
      661 ccgccttgtc gggcggacct gcgccgagcc gcggatcggc gccctccttc agggcgccgc
      721 cgttttggac ctcggcctgg acgaggccct ggcggccttc aagacgccca tctcgcggta
      781 aggggagagc atggaaccca aggccatcat cgtcaccggc aacggcatca actccgacgc
      841 ggagctcgcg gaggccttcc gcctcgccgg cgcggcgccc gagcgcgtcc atctctccga
      901 cctggtcgcg gagccggagc gactgctccg cgcccgcctc ctcggcttcc cgggcggctt
      961 ctcctacggc gaccacctgg gctcgggcca ggtcttggcc ctcctctgca ggagggcgct
     1021 caagccggcc ctggagcgct tcgtcgccga gggaggcctg gtcatcggcg tctgcaacgg
     1081 cttccaggcc ctcgcgaaga tgggcatgct gccgaaccgc gggcccgcgg cggggctcca
     1141 gccctgggag cgcgaggtca ccctggtgca caatcccgac gcgcactacg tcgacaagtg
     1201 ggtgcccctc cgcttcgagc ccgcctcccg ctgcgcctgg acccgcggcc tcccgccgcg
     1261 cctcctcccc atccgccacg gcgaggggcg cttcgtcgcc cgcgacgccg cgaccctggc
     1321 cgccctggag gacgagggcc tcgtggccct gcgctacggc ctcacgggcg gaaggcccgc
     1381 gccgggagcc gccccctcgc ccgacaaccc gaacggctcg gaggccgaca tcgcgggcat
     1441 ctgcgactcg acgggccggg tcttcggcct catgccccac cccgaggcct tcctcgtccc
     1501 ggagaaccat cccgaccggc gcagggggcg ccgcgacccc gtcggcctgg acatcttcga
     1561 gaacggggtg agggcggccc gcgaggcgac ctagccgccg gacggatcgt cggccaaaac
     1621 aaaaggctgg cgctccgacc ggagccgggg tatactcgcc cgcatggcct cttgggaggg
     1681 gcaaaaaagt acatgagcga catccctgag cctggggcgg ccctcgccga ttccgagcgc
     1741 gacgactacg agaagcagat cttcgacctg cgccagcttc tcgaagtctc caagagcctc
     1801 aactcgaccc tcgactacaa catcctcatc gactccatcc tcttcaccat catggggcag
     1861 atgaaggtga tcaaggccgg cctcttcgcg aagaagggca tcgacacgcc ctacttcagc
     1921 ctgcaccgca actacaaggg cttcgacgtc gaccaccggg tcaactactc gatctccgag
     1981 gaccaccccg cgatcaagct cttcacgcgc cagtacggct gctacacgct ggaggacatc
     2041 aagcggcgct tcggctccct ccagggcctc gagggcctgg cttccctgga gccctccctc
     2101 atcgtgcccc tcaaggccaa gggcgtcatc aacggcatcc tggtcctggg cgagcggatc
     2161 gacgactcgg gcttcgacgc ctacgagcgc gagtacatcc tgaccctcgc ggccctggcc
     2221 gcggtcgcca tcaacaacgc cttcctcttc gagatgacga cgacggacat gatgacgaag
     2281 ctcaagatga agcactactt ctacacggtg ctcatcgaga ggatggagca ggcggccctc
     2341 gcgggcaagc cgctgtccat cgtgatgctc gacatcgact tcttcaagaa attcaacgac
     2401 acccacggcc acctctgcgg cgacgccgtg ctcaagcagg tggcgaggat cctccagtcc
     2461 tgcgtcaggg ccgtcgacct ggccgcgcgc tacggcggcg aggagttctg cgtcctcctg
     2521 cccgacgccg accgcgagcg ggccaaggcc gtggccgagc gcatcaggca gagcgtcgcc
     2581 ggcacgccca ccgagtacga gggcctctcc ctcctcgtca ccgtctccgt cggcgtcgcg
     2641 cagtacgatc cggcccgcga cgtctccgcc aagtccctca tcgaccgcgc cgaccgcgcc
     2701 ctctacttct ccaagcagaa cggccgcgac cgcgtcaccg cggcggagta gcgccgtggc
     2761 gcggctctcc ttccgccctt gggccggccc ggacgccgac gagggccgag gcccggccgt
     2821 cgggacgcgc ggctcggcgt tcgctggtgt cgccggcgct ccagccgcgg gcgccggccg
     2881 cgcccccagc gaggccggcc cggcccccct cgacgcacag acctctccgg ccttcagcct
     2941 cctcgtgtcc ctgcagcgcg cgggcgcgcc cctgcgccac gactgcggcg gcaaggccct
     3001 ctgcggcacc tgccgcgtgc gggtcctctc gggggcggag ggcctttcgc cgatgggcga
     3061 gcgcgagcgg ctccggctgg aggcggtcgg cgccccgctc gacggctcgg tccggctcgc
     3121 ctgccaggcc cgcgcctcgc gcgacgccga gatcgaggcc ctgctcgggc ccgcggaagg
     3181 gaagggggag gggcgatgag gcattggtgg gaggacgcgg tcttctatca aatctatccg
     3241 cggagcttcg cggactcagg aggcgacggc gtaggcgacc tgccgggcat acgctcccgc
     3301 ctgggctacc tcgcggagct gggcgtcgac gcgctgtgga tctcgccctt cttccccagt
     3361 ccgatgaagg acttcggcta cgacgtctcc gactaccgcg gcgtcgatcc cctcttcggg
     3421 accctggacg acttcaaggc cctctccgcg gaggccgggc ggctcggcct ccgcatcgtc
     3481 ctggacctgg tcgcgaacca cagttccgac gagcacccct ggttcaaggc cgcccgcgcc
     3541 tcacgggagg ccaaggagca cggctactac ctctggaagc cccttgaggg gaaggtcccg
     3601 aacaactgga agagcctctt cgagctccgc tccgcctggt acccgaatcc gcagactggc
     3661 gagcgctacc tcgccacctt cacgcggagc cagcccgagt tcgactggcg gaaccccgag
     3721 ctccggcgcg agatgtatga cgttatgcga tactggtacg gcctgggagt ggacggcttc
     3781 cgcctggatg tcgcgaccgc ctacttcaag gacgcggagt tccgctcgaa ccccttctcc
     3841 tggaaggcga accccgacct cttccagcgg cacgtctacg accgcaaccg ccccgaggtc
     3901 cacgaggtct tccgcgagat gcggaaggtc gccgacgagg cgggggagcg cgtcctcatc
     3961 ggcgagaccc acggccagga ggccgagctc gccgcctcct gccacggcga ggccggcgac
     4021 gggctccaca tggccttcaa cttcgacttc ctgaaccagc cctggagcgc cgcggccttc
     4081 cgccgctcgg cggaggcctg gtacgcggcc cttcccgaag gcgcctggcc caacttcacc
     4141 ctctccaacc acgaccgccc gagggcggcc ttccgcttcc gctcccgcga tccccgggtc
     4201 acggagggac gggcccgcgt cgccgcggcc atgctcctca ccctgagggg cacgcccttc
     4261 gtctactacg gcgaggagct cgcgatgggc tgcgtccgcc tgcccagggc cagcctccgc
     4321 gaccccctgg ggatccgcac ctggcccctg ggcttcctgg gccgcgatcc cgagcggacg
     4381 cccatgcaat ggggctccgg gccgggggcc ggcttcacga agggcgagcc ctggctcccc
     4441 ctcaatcccg actggcgcga gaggaacgtg gaagcccagg ccgccgatcc ggcctccacg
     4501 ctcgcctggt acaagagcct cctcggcctg cgccgctcgc gggaagcgct ccgctcgggc
     4561 tcgatctcct tcctggacgc ggggagggga tcccgggcct cggaggtcct cgcctacgaa
     4621 cgccgcctcg gggtctcggc ggcaggccag gcgggcccgg gaggagccgc gggcgcggga
     4681 agcggcgccg ccgagggctc ggccgcgggc ccggccgagg gcccggtcgc ggccgcggca
     4741 ggctccggcg ggcgcctctt cgtcctcctc aacttctcct cgaggccgag gaaggccggg
     4801 cttcccggca gggcccgggt cctcctgggc ggcgcacccc tggacggctc gccccgaacc
     4861 gcgggcgaag tcctcgggcc tgacctcgag ctccgcggcc tcgaggccct ggtcctggag
     4921 gcgctctagg cggagggaac cgagagctcg agcctgccct tcttgaccat cgcgccgtag
     4981 acgaagagct ggaggagggg gcgctcgagg accgagcccg cggcgcgggc ccgcgcgtcg
     5041 atctcggcgg caaggccgat gatccgctcg cagtcgcgcc tttcgtagcg cgcgagggcc
     5101 ttgcggtgca gtccctggag gctcttggcc cgtatcccgc tcttgaggca ggccgtctcg
     5161 aagccctcgc cgccttcgag gaggaggtgg agccgcaaga ggcggcggaa gctccagacg
     5221 agggcggaga ggatctgcac cgcgccgccc tggcggtcgg ccaggacggt ctccaggcac
     5281 tccagggccc aggcgggctc gtcctccgcc atccgcgcga agaggctgaa ggcgtcctcc
     5341 tggcggttcc tcgcgacggc ggcctcgatc tccgcctcgc ccaggacgga gccctgggga
     5401 aagaggacgg agaggcggga gcaggcggac tcgagggcgg aggtgtcgtt ctccacgagc
     5461 tcgaggaggg cctccacgcc ctcctcgtcg atgcccaggc ccagctcgcg gaggcggcgc
     5521 tcgacccagc gcggcttctc gctctcgaag agctcgtaga aggtcttctt gcgttccttg
     5581 ccgacggcct cctccacggc cttctccgcg tagaaggcgt cggtgacgag gaggaggacc
     5641 gcgtcggggg cgggcgaggc gaggtaggcg gcgagggcgc ccgtgtcctc cttgcccttg
     5701 atcgcctccg ccccgcggta ctcgacgagg cggcggctcg cgaagaggga gccgttccgc
     5761 aggagggcca ggagctcgcc gaccccggcg tcggtcgagt agaggcggtg ctcctccgcg
     5821 ggggccccgt cggcgcgggc gatcgcctcg cggagctcgg cgacgaaggc cgaccgcttc
     5881 ccggtctccg ggccggcgag gaggtagcag ggctcgatct tcacgctagt atttccggat
     5941 gaggcccctg aggcggccga cgatgcgcac gtcctgggtg aagatcggcg agtaggccgg
     6001 attctccgcg acgagcttca cgcggttgtt ctcgcggtag aagcgcttca gggtcactgc
     6061 gtcgtcgatc atcgcgacca cgatctcgcc gttttccgcc gactgccgct cctcgatgac
     6121 cgcgatgtcg ccgttgaaga tgcccgcgtc cttcatggag tcgccgcgca cggtcagggc
     6181 gaagcaggag gaggagcgga ccatgtccgc cggcacgggc accgagcccg agaagttctc
     6241 ctccgcgaag acgggcttgc ccgcggccac gacgcccagg agggggagct ggacgacgga
     6301 gcgctccgcc cgctcgggcc gtatgatctc gagggcccgg gaacggtttt cccccagcct
     6361 gagcttcccc ttcttctcca gggccttcac gtggtcgtag gcccccttca cggagatgga
     6421 gaagctgtcg gcgatctcgc ggatcgtcgg cggataggcg tgctcctcga tgaagtcggc
     6481 gatgaagtcg aggacttcct gctggcgaag ggtcaggctc ttcatttttc cggctcgatc
     6541 cccaatttct tgagcttggc gtggaggccg ctcgggtaga ccccgatcgc ctccgccgtc
     6601 ctcgatacat tatatccgca ttcgcgcagc ttgtgcacga gatattcccg ctcgaagagc
     6661 tccttggccg gctggaggcc gagcttgagg aagcgcggcg cgacgagggt ctccttggcg
     6721 ccgccgccct ttccctcgcc gttcccgccc gctcctccgg tccgcgcgcc ggcctcgccc
     6781 aggacgcgct ccacctcggc cgcgccgatt ggctcctcgt cggagaggac gcggagccgc
     6841 tccacggcgt tcctgagctc tcggacgttc cccggccagg gcctggcggc cagggcggcg
     6901 agggcgtccc cggccaggct cctgccgccg cccgagagct cgaggaagcg gcccacgagg
     6961 gcgggaaggt cctccaggcg ctcgcgcaag gcgggcatcc ggatcggcac gacggccagg
     7021 cggaaataca ggtcctcgcg gaagcggccc gcggcgacct cctcgcgcag gtccttgttg
     7081 gaggcggaca ggacgcggac gtcgacggag acggtctcct cggagcccag gcgctcgaag
     7141 cgcatctcct ggaggacgcg gaggagcttg gcctgggcgg agggggagag gtcggcgacc
     7201 tcgtccagga agagggtgcc gccgtcggcc gcctcgaagc ggcccttgcg ccgagagacg
     7261 gcgtcggtga aggcgccctt ctcgtggccg aagagctcgc tctccagcag gccctcgggt
     7321 atggccgcgc agttgacggc gacgaagggc gcctccgccc ttccgctctt cgcgtggatg
     7381 agccgcgcga cgagctcctt gcccgtgccg ttctcgcccg agatgaggac ccgcgcgtcg
     7441 gaggccgccg cctgctccgc gaggctccgc accgtcgcca tcgcggccga gccgccgacg
     7501 agctcctcgc aggcggccct gccggcgctc ttcaggctcc ggttctcccg cctgagcttc
     7561 gtgatctcga gggcgttcct gacgccggtg aggaggcggt cgatggagag gggcttctcg
     7621 atgaagtcga aggcgccgag cttgatcgcc ctgacggcca tgtcgatggt gccgtggccg
     7681 gagatgacga tcacctcgag ctcgggccgg cggcgcttga attcggccag ggcctcgagg
     7741 ccgcccatcc ggggcagcca cacgtccagg aggacgaggt cgacgcgctc ccgctccagg
     7801 atctcgaggc cgacgagggc gtcctcggcg gcgaaggcct tgtggcgctc gtcctcgagg
     7861 atgccgcgca cggcgctgcg gatgccgggc tcgtcgtcga tgatcaggat gctcgccatg
     7921 ggctccctcg aggctccttg gggcgccctt ccggggcccc gctccgccgg cgccgcccct
     7981 cgctcagggg ccggccgcgg gaaggtctat gtagaaggag gtgccgacgc cctcctcgga
     8041 ttccgcccgg atcgagccgc cgtggtcggc gacgatgcgc tccacgatgg agaggccgag
     8101 tcccgtgccc gcgggcttgg tcgtgaagta gggagcgaag atccggccca ggacctccgc
     8161 gggcatgccg cggccgtcgt cgcgcacgag gagcctacaa tatctcgatt cggcggtctt
     8221 tacaagctcg gcggcgaact cgacccggcc cgagccctcc atggcgtcca cggcgttcga
     8281 aaggaggttc gagagggcgc gcttgagctg ggcccggtcg gcgaggagga ggatgccctc
     8341 gggcaggcca gcgagctcga agcggaggcc tgggtaggag gccgcgtaga gggcgaccgc
     8401 ctcctccacg acgccgcgga gctccgtcca gccgcggacg ggctcgggca gggccgcgaa
     8461 ggagcggaag tccgagagga gggcgtccat cgactccacc tccgcgacga tggcgagggc
     8521 cgagggctcg atgagctcga gggccctctc ggggtccttc cccgcgaggc ggaggaggcg
     8581 ctcggccgcg aggcggatcg gcgtcagggg gttcttgagc tcgtgggcca ggcgctgggc
     8641 tatgtccttc caggcgtcga tcttgccctt cctgaggtcg ccttcgcggt agcgctcgat
     8701 cccgccgagc atcgcgttga aggaggcgac gaggcggccc gtctcgtcgc cgggcttggc
     8761 caggaggcgc agggagagct cgcccgaggc gacgcgcctg gtcgcctcct ccagggaggc
     8821 gaggggctcg acgacgagct ccgccgcggc gacgccgaag agggcggcga ggaggaggag
     8881 gggaaggacg aggtaggcgt agaggaggag gaggagcctg ccccagcggg gcgagaaggc
     8941 ggccatgagc tcggcctggc tcttccccct cgcgagctcg cccgcggttc ggtcgaagcc
     9001 ctcggggagg cgcagggtga ggacgacggc gcccgagggg ccccagggga cgaggtagcg
     9061 gaggcggccg acgccgcctt cctggctcgc ggggagaggg cccggcgccg cggcggccgg
     9121 ggacgcggcg gcgccgggga cttccgcgag gcgggcggcc cggtcccccg cgaaggccgc
     9181 gctcgcgccc ctttcgaaga gttcgacggc ctccatccgg ggctcgcggg cgcggagggc
     9241 ctcgagaagg cggtcgggcc gctccgggag cctgcccggg gcgagggccg gcaggtcgtt
     9301 cctcgcggcg tactcgagac gccgcaggtc ctcgtcgtag gcggcgagga cgaggcggag
     9361 gccgccctcc aggccgcgcc tgatgtcggc ggaggcggcc tgggactggg cccgccgggc
     9421 gagcctcagg aggaagacgc cctgggggat ggaggaggcg gcgacggcga ccaggaagag
     9481 ggcgaccagg cgggccttga gccgggagcc gtagcgccgc cctcggagct cggcggcgag
     9541 gcggcggaga cggtaggcga agacgaggag gaagccggcg ggcaggacgc cgaggaggag
     9601 gagggggagc ctctcctcgg ccccggggaa ggatggcgcc tcggagagga cgccccgcgc
     9661 atagacgtag gccccgaggg cgacggcggc gtagacgacg aggagggcga ggaggtcggt
     9721 gaggccgcgg ctgcgcttgg gagaatcggg tgcggggcgg cgggcgcctg atcccggagc
     9781 cccggcggcc gcggctaccg ggcttggggc cgcgccggag gcggcgtccg ccgtggccgc
     9841 cggtgccgcg ccggaggcgg cgtccgccgt ggcgggatcc gtacccgcgg gcccggggcc
     9901 cccggccccc cctccgctca ttcctcctcg aagcggctct cgaaaagccg ctcgatggcc
     9961 gcggcggcct cctcctcgtc ggcgccgtcc gcgaggatgc ggatgggggt gccgaagctc
    10021 gccccgaggg tgatgatgcc catgatggac ttcgcgttga tcttgtcgct tcccttctcg
    10081 agccagatct tggagccgaa cttgctcgag gtctggacga tgagggcggc cggcctcgcg
    10141 tggatgccgg cccggttctt gatggtcacg ttgcgctcgg tcatggaagg ccttcctcgg
    10201 ttcgggcttt cgcgggcgcc gtgttccggg ccgcccgccg gcttggttcg ctagaggtaa
    10261 tcgtcccggt tgaagtacgc ggcccgggcg ttctcgctct cgagccagcg caggatgttc
    10321 tggttgaact ccttcgccga gtggtagccc atcttcttca ggcgctcgtt catcgccgcc
    10381 gtctcgatga tgatgggcac gttgcgcccc ggcttcacgg ggatgacgag ctgggggacg
    10441 cggacgccca ggatgtccgt ggtcttctcc tcggtgccga tgcggtcgta gaccttgtcc
    10501 gcgtcccact cctccaggtt gcagacgagc tggatctgct tcttgtcgcg gatggcgcct
    10561 acaccgaaga ggtgggtgac gttgatgatg cccaggcccc ggatctccat gtggtggccg
    10621 atgaccttgt tcgcgccctg gcccatgagg atggagccgt tcacgcagcg gatctccacg
    10681 acgtcgtcgc agaccaggcg gtggccgcgc tcgacgagct ccagggcggt ctcgctcttc
    10741 ccgacgcccg agtccccggc aaggaggatg ccgatgccgt agacctccac gaggacgccg
    10801 tgcagggtcg ccttgggcgc gaagatgtcg gagaggacgc ggaggatgcg gctcgtgagc
    10861 tcggaggagg agaggccggt ctggaggacg gggcagccgc cctcctccgc gaggcggaag
    10921 aagttccggc cgggcgcgag gttgtgggtg aagatgcagg ccgggatcgg gtaggagaag
    10981 agcttctcga agctctcggt cttgtcctcc tcctcgatct tcttgaggta ggcgttctcg
    11041 ccgcggccga agacctggag gcgctggaag gcgaaggact cgaagaagcc cgagagggcg
    11101 agcccggggc ggttcaggtc ggggacggtg atctcgcggg agaggccctt cctgccgcag
    11161 atgcagcgga ggtcgagggc gttctgctcc ttcaagtcga ggtcgaggag gtcgaggacc
    11221 gtgaagcgct gctcttccat ttgtaggcca atcctactac gaaagcgccg cgcgcgcaaa
    11281 cggggggcct tggggcgccc gcgcgggctc cgccattccg tgccccgcgc cggcctggcg
    11341 gcccggagac ggcgcggccc cgggggatcc ccggggccgc cttcggtccc ttccggagcc
    11401 tagcgcttct cctggacctt ctccttctcc ttcgtgatct tctgctccag cctgtcgatg
    11461 agcttgtcga tgccggggtt cacgtcggcg tcccgctcct cgacgtgggc ctgggtgccc
    11521 caccggaagt tgacggtggc ctccagcttg aagtccttct ccttcgtgaa gacgaagatg
    11581 agatcgacga ccttgtcgtc cgcgtacccg atccgctcga gtttcttgtc caggtagtcc
    11641 ctgctcgtgt cgctcaggtt gaaatggacg gaacggacgt cgacggtcat gcatgcctcc
    11701 ttctcgctcg gggctcccgc ctgccgggtc ccggaggcgt cctggggcgt cgcccagggc
    11761 ggcccggggg ctccggcgcc ggtcagccct ataacgtcgg ccccggggcg gggccgggcg
    11821 tctccttccc tacagccgct tgcggccgaa cgacgagtcg aggtcgagct ccccgcggta
    11881 cttggccacg gtgcgccgcg cgagcgtgat gccgcgccgc gcgaggtgct cggcgatgtc
    11941 gcggtccgag agcgcggaat cctcgccctc gatgatctcc ttgatgacct gcttcacgcc
    12001 ctccttggag aagcgcgagc ccgaggagcc ggcgccggag atcgagttcg tgaagaagta
    12061 gcgcagctcg aagatgccga agtcggtctg gaggtacttg gagtgggcga tgcgggagac
    12121 ggtcgtctcg tggacgccga cctccgcggc gacgtccttc agggtgaggg gggccaggtg
    12181 cttgggcccc ttcgcgaaga aggcgcgctg gaactccacg acggcccgcg cgaccttgag
    12241 gagggtctgg ttccgctggc ggatcgagtg gatgaagaac ttggcccgct tcacgttgtc
    12301 gcggacgtag gcgcccgtcg ccttgtcgcc gcccttcttg tccgcgaggc tgtcgaagaa
    12361 ggggtcgatg cccaggacgg ggatctcctc gtcgttgagc acgatgacga agtcgccgtc
    12421 cttgagcttc acgaggaggt cggggaggac gtagcgcggc tcctccttgg agtaggcgcg
    12481 gccggggaag ggagagagct ccttgatgaa ggcgagggcc agggcgagct cggcctcgcc
    12541 cagcttgagg cgcttctgga tctccgcgtg ccggcccttc tccaggagct cgacgtggcc
    12601 gtccaggatg gccagggcgg cggcgggggc cgcgggggcc agggccgcct ggacccggag
    12661 gctctcccgg tagtcggccg tgcaggtgcc cgccggctcg aggccgcgga ccagggccag
    12721 ggcctcgtcc accttgccgc gcggcacgcc gggcttgagg ctgtaggggt cctccttgtg
    12781 gaagccgtcg tcgtcgaggt tctggatgag ggcctcgcct atggcgcggg aatcctcgtc
    12841 caggggctgg aggcggagct gccagaggag atgctcctgg agggtctccg ggcgggcgat
    12901 ggccccctcg atgaacatcc gcttggagtc gtcgtcgagg ccgg
//
LOCUS       DAOOSJ010000044        12907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3254_length_12907_cov_6.254902, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000044 DAOOSJ010000000
VERSION     DAOOSJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_12907_cov_6.254902"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLG14_03655"
     CDS             complement(<1..579)
                     /locus_tag="PLG14_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48258.1"
                     /translation="MRLSRSAASFLALAFLFAGGYPRGAVAQGAAQGAAAGAAQGPAR
                     PASPAASGADQSAATPAQTAPPGAAAVSASALILGPEDLRIEQLTDGGYHVYVRRKPG
                     LGSVLLTESTKDPARKADSYAYRAAEWNPVNGDERRILDGAFIPPEKKLYSIIDSSPE
                     PDPVFGEAYHLFIPWVVEWGYAWSRNGRTFVSD"
     gene            complement(612..1898)
                     /locus_tag="PLG14_03660"
     CDS             complement(612..1898)
                     /locus_tag="PLG14_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX48259.1"
                     /translation="MRNLALLLASAAAALALSACAAPAAAEKGPPPASRFRAEAESVV
                     ARLSLDEKCAQVLLVGVGGRGSPSADSLELVSSLAPGGVVLFGFNIGEEASSLGPFVA
                     RLQDAAAASAPRLPLLVALDHEGGSVFRFRSGVTRLPPPAEVGARGAPYAGLLGERAG
                     LELKALGVNLVLAPVVELLDADNRALLGGRSYGAEPAVVDAAAGAFIRGLQASGVAAT
                     AKHFPGNAGLDPHKGLPVLAASKAELEASYFPRFAAASRAGVGAVLLSHVLVPAMDAK
                     RPATLSPALIEELKGRLRFEGVVLTDDLFMGAVAGERPPERSAVEALAAGADLLMLSA
                     GGSAARVRQAIRRAVESGALPEARLDDACARVVAMKLRFSLAEAFDREARAERLAAFP
                     GLVEESSLLLRARLAEAGKSGGKAPSFPAAAGTKTP"
     gene            2048..2701
                     /locus_tag="PLG14_03665"
     CDS             2048..2701
                     /locus_tag="PLG14_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOX48260.1"
                     /translation="MDELREAAFCPTGRKQGDVAAAVIHIVGGALAAAGLPVLVVGAA
                     AERAAALVVGFSLYGASLLYYFAISTLAHFLEAPGARRVFATLDEAGDFFLIAGSWTP
                     LCLTVFKGPLGWALFGVLWGLALFDFLVALLYTSRAKNLVLAAYYLGVTLVLPLVAPF
                     RGLLGEILFAWLVLAGLLYAAGLLFRSRDEMPYHHGIWHAFTLAASICHFFGLLSLL"
     gene            complement(2807..3829)
                     /locus_tag="PLG14_03670"
     CDS             complement(2807..3829)
                     /locus_tag="PLG14_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOX48261.1"
                     /translation="MQRILPIASGKGGVGKSVLTANLGLALARAGKTVVLVDLDLGAS
                     NLHTCLGLRNRHAGLGSFVWKAEKSLSGLLVETGFERLWLVPGDNLLPGTANLEWNSK
                     RRILKELSKLPADFILLDLGAGSSYNVVDFFVAAPSGLLVLCPEVTSVLNAYSFLKTA
                     AFRILHRSFPEGSEERAEVLAYAGTKTEGSGLSFLDFARGLAERRPERGGPALEALSR
                     LLPRVVLNRGKSPEDAELGYRLRDISRKNLGLSLEFSGFLLEDPGVAKSVAARRPLLD
                     LDASSPFARGVQALATRLAQAPEASPPLATEGPDGEAEAELAVAAAGQNEGEDELARL
                     AAEAFA"
     gene            4083..>5755
                     /locus_tag="PLG14_03675"
     CDS             4083..>5755
                     /locus_tag="PLG14_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOX48262.1"
                     /translation="MAAPFPSALATRPGRKAPASAAPAAILVAAAFLALALPAAAAES
                     PRPSVETEATAAEGLAGLSVRAFASDTGEELKGAFGGAELSVNGAVVGRTPYSDARIP
                     PGTYLLRVEAEGYYPSEILLGLAEKTLYKLVFHLARMTGYLAVEVSPPDAEILVDGKS
                     RRPGLIELPTGGRRLVVRRFGYAEREYALSIEDRATSFLRVELEKAPFAVSELRSSRA
                     AFNPRNAGLLGRCALDFEVSSYGSGRLSILGPSGDAVAAAAFPSFETWRQGFVWRGRG
                     ADGLPLPDGAYLARLEAWPAAPEGAAGSAAPPLVREVAIGLDSRIVVEPRAVQAASAG
                     LLYFPDPRALPAGSLALELSASGGADFRTPPAFGLGASYSLGRAGLALALTAEPGESG
                     GEAGAETALSLSFALVREPGPFAAALHLRGSWSSAVAPIMAGAYPGGLAELALPAALS
                     LGAFRLGFAPGISAAIAADGSEAAPRPFLRAGAWLSGSRFLAGLSGELATGGLGKSPL
                     EPDWPARLALEGRLVLDPSPLLVGGGLFLDLEPGADPALRLGLALGLLL"
     assembly_gap    5756..5855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5928..7196
                     /locus_tag="PLG14_03680"
     CDS             5928..7196
                     /locus_tag="PLG14_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOX48263.1"
                     /translation="MRSTVSIVPCPEYEAKALDRAVAEAVELAGGFEARGAAVLLKPN
                     LLNANGPERGVTVHPAIVAAAVRWLKVAGASRVLVGDSPGWQTQELVGRKTGVKDAVL
                     QSGGEWADFSTEAEFELPEGRLARRFRLAAPLAEADLLVSLPKLKTHQLMYYTGAVKN
                     LFGLVPGLQKSAFHLRFPGRGEFAGMLSDLALASAPDFSLMDAIVAMEGPGPNNGRPR
                     SLGLVLASRDPLALDWTAAGLIGYEPGTVPYLADLMGRMAAKAGDAARPERGPAPESA
                     REPAEDGARNGGRDEAPEASRRRAFPSWAASPAEIVLAGLPFEEAKPEAFELVPLLKE
                     NDFLSKKLPPWLHRLVKGAMVPRPRFDHGKCVRCSGCVRICPAKALVLAPSARDPRGR
                     IEIDYERCIRCYCCHEICPEDAIDLVRRWL"
     gene            7196..8962
                     /locus_tag="PLG14_03685"
     CDS             7196..8962
                     /locus_tag="PLG14_03685"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOX48264.1"
                     /translation="MEAQVPWDGLLDFGPSSAYRRLDLPPPEPFAAQRRGGAGESLLY
                     VEEASLAALAREAFEEIAFRLPTGQLEGFAAVLGDPEASSAERFVAASLVRNAAVAAE
                     GVLPLCQDTGSAVAYAWKGAGIASSGRDEEALAAGAAEAYAARRLRASVLAPTGFLSE
                     RNTGSNLPALVDIRQAAGHEYRFCFAAKGGGSQNRTSLSMEPPSLLSEAALEKKLAAL
                     VAALGASGCPPYRISLVLGGRSPDEALYALALAGLGLLDGLPGTPDASLADAGAGGAD
                     LRGGPGAGRGEGRPRGLGLPLRDRGWEDRITRLAAATGVGAQFGGSRLALDARAVRLP
                     RHAASLPLAFGVACAAHRRARAVVRADGFFLERMEADPARFLPRELPLFEGAARVDLD
                     RPLGELAALLGSMRPGAPLLLSGTVVTARDAAHARFRALLAEGLPLPGYLLRHPVFYA
                     GPTEAAPGEASGSFGPTTAGRMDSYLDALMERGASLVTIAKGGRSPAAAGAIGRRGGA
                     YLAVIGGAAALAARESVVSSEVIDHADLGMEAVRRVVLKDLPAILAVDGSGRDFYAEL
                     AAGGSAAAGPGAAKGGDAGAGR"
     gene            8959..10164
                     /locus_tag="PLG14_03690"
     CDS             8959..10164
                     /locus_tag="PLG14_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48265.1"
                     /translation="MRKGAFRLGLGYFSFLAVFGVSTPYLQILLRGLGYGPAAVGVLL
                     GVFELVGIASPFFVGKIADSRGRSRPVLAASCLATLAALPLLVLVRGVAATVAGLVLL
                     AVGLKALAPLMDAVSTNYAESRPELGIRYGNLRAAGSAGFILVALALQALPGFGEGSP
                     ERIALAMGLLAAAFLAYLPLLPETAGGPRRPKPGPAGRRGFAPGFGLGLAVIALSRVA
                     MAPVSSFFSLYVKEELRWNAVGALWALGAAAEIPLMLLAHRIVARLGAMGAIAVSTAA
                     VALRLAVCAAFPSPAGAVAAQLLHALCYGLFQPAAVSFVALSAPPERRASAMALYMSL
                     GVGLPTFLGSALGGLVVEGSGYRVLFASYIPFALASITLWAAGGKRLAGSPRAPSGGP
                     RPSRRRADA"
     gene            complement(10112..10582)
                     /locus_tag="PLG14_03695"
     CDS             complement(10112..10582)
                     /locus_tag="PLG14_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48266.1"
                     /translation="MGEGDYAAYRGACVEELARALAESQGLSLDAAAARAAAEQDELL
                     PAGEATPGQRLFVARLAPGGKAVGRAWFGEVPGGEEPGTAFLFHLEVAPAFRGRGLGA
                     ALLDGAEARARALGFARMRLYVLSSNAAARALYARAGFRRLRGDGKGEVLRKEL"
     gene            complement(10699..11286)
                     /locus_tag="PLG14_03700"
     CDS             complement(10699..11286)
                     /locus_tag="PLG14_03700"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOX48267.1"
                     /translation="MVAGDGSAGRKWKRLSERKLLDCPIFTVGELESESPDGRRGRFV
                     RLEAPDWATVVPFLPAGEGGALPSFLMVRQYRHGSDEASVEFPGGVVEPGEDPAAAAA
                     RELEEETGWRAGRIRSAGWTYPNPAFMGNRFHVFVADRLEPTGRRALDEHEIVDAFLV
                     PAVEVRELMGSGEYRHALMAAALLMAERVLEGGEG"
     gene            11548..11883
                     /locus_tag="PLG14_03705"
     CDS             11548..11883
                     /locus_tag="PLG14_03705"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX48268.1"
                     /translation="MQDWALEFPGAVTVCDRDFTIIYMNDKSAKTFAKWGGEGLLGKS
                     LVDCHKPESIATMRRILETGEPNVYTIEKEGQRKLIYQAPWRKDGAIAGLVELSLVVP
                     AEMPHFVRS"
     gene            complement(11970..12503)
                     /locus_tag="PLG14_03710"
     CDS             complement(11970..12503)
                     /locus_tag="PLG14_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOX48269.1"
                     /translation="MTEKTFMAGFGGQGIISLGQLWVYCAMKEGLEVTFFPFYGAEKR
                     GGVARASVIVSTKEIASPLVTSPDSSIVMNIDSLPICEEVAKQGGLMLVNSSLVKVDP
                     KRKDLKVLKIACNDIAEQIGDAKIANMVMMGALSRATGAVKLDKLEAVLKSFFPESKH
                     KFIPMNMAAIEAGMKAV"
     gene            complement(12500..>12907)
                     /locus_tag="PLG14_03715"
     CDS             complement(12500..>12907)
                     /locus_tag="PLG14_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX48270.1"
                     /translation="IFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDPVDAGMGYPIQ
                     VCELLATLGGTRYLARGAVNTPANVRKTKQYIKKAFEAQLRGEGFGLVEILSPCPTNW
                     QMKPGEANEWLEQNMMPYYKLGEIKNTLEEAAK"
BASE COUNT         1582 a         4790 c         4660 g         1775 t
ORIGIN      
        1 gtcggagacg aaggtccggc cgttccgcga ccaggcgtag ccccattcga cgacccaggg
       61 gatgaagagg tggtaggcct cgccaaagac cggatcgggc tcggggctgg agtcgatgat
      121 cgagtagagc ttcttctcgg gagggatgaa ggcgccgtcc aggatgcgcc tctcgtcgcc
      181 gttcaccggg ttccattcgg cggcgcggta ggcgtagctg tcggccttcc tggcggggtc
      241 cttcgtgctc tcggtgagga ggacgctccc caggccgggc ttccgccgga cgtagacgtg
      301 atagccgccg tcggtgagct gctcgatgcg caggtcctcg gggccgagga tcagggccga
      361 ggccgagacg gcggccgcgc cagggggcgc cgtctgggcc ggcgtggccg ccgactggtc
      421 tgcgccggaa gccgcaggcg acgcgggccg ggccgggcct tgtgccgcgc cggcggccgc
      481 gccctgcgcc gcgccctgcg cgacggctcc gcgcggatac ccgcccgcga agaggaaggc
      541 cagggcgagg aaggaagcgg cggaccttga aagcctcacc ctcccatcat cggctggcgg
      601 ggaggcccgg tttagggagt cttcgtgccc gccgccgcgg ggaaggaggg cgccttgccg
      661 ccggacttgc ccgcctcggc gagcctggcg cggaggagga gggaactctc ctccacgagg
      721 cccggaaagg cggccaggcg ctccgccctc gcctcgcggt cgaaggcctc ggccagggag
      781 aagcggagct tcatcgcgac gaccctggcg caggcgtcgt cgagcctcgc ctcggggagg
      841 gcgccggact cgacggcgcg gcggatcgcc tggcgcacgc gggcagccga gccccccgcc
      901 gacagcatga ggaggtcggc gccggccgcg agggcctcca cggccgagcg ctcgggcggc
      961 cgctcccccg cgacggcgcc catgaagagg tcgtcagtga ggacgacgcc ctcgaagcgc
     1021 aagcgtccct tgagctcctc gatcagcgcg ggcgagaggg tggcgggccg cttcgcgtcc
     1081 atcgcgggca cgaggacgtg ggagaggagg acggcgccga cgccggcccg cgaggccgcg
     1141 gcgaagcgcg ggaagtagga ggcctcgagc tcggccttgg aggccgcgag gacgggcagg
     1201 cccttgtggg ggtcgaggcc cgcgttgccg gggaagtgct tcgcggtggc ggcgacgccc
     1261 gaggcctgga ggccgcggat gaaggcgccg gccgcggcgt ctaccacggc gggctcggcg
     1321 ccgtaggagc ggccgcccag gagggcccgg ttgtccgcgt ccaggagctc gacgacgggg
     1381 gccaggacga ggttgacgcc cagggccttg agctcgaggc cggccctctc gcccaggagg
     1441 ccggcgtagg gggcgcccct cgccccgacc tcggccggcg gcggcagccg cgtgacgccg
     1501 ctccggaagc ggaagaccga gccgccctcg tggtccaggg cgacgaggag gggaaggcgc
     1561 ggggccgagg cggccgccgc gtcctggagc cgcgccacga aggggccgag ggaggaagcc
     1621 tcctcgccga tgttgaagcc gaagaggacg acgccgcccg gcgcgaggga ggagacgagc
     1681 tcgaggctgt cggcgctcgg cgagccccgc cctcccacgc cgacgaggag gacctgcgcg
     1741 cacttctcgt ccagggagag ccgggccacg acggactcgg cctcggcgcg gaagcgcgag
     1801 gcgggcgggg gccccttctc ggccgcggcc ggggcggcgc aggcggagag ggccagggcg
     1861 gcggccgcgg aggcgaggag gagggcgagg ttgcgcatcg ggaggactct agcccggccg
     1921 gggccggggc ggcaaggagg cggaggctcc aaggctcggc ccgcccgtcg cgggctaggc
     1981 gccggcgcgg agcctcgccc ttccgggggc tggacaggag ggcatcgccg gcgctctact
     2041 tgtgccaatg gacgagttgc gcgaagcggc cttctgcccc accggccgga aacagggcga
     2101 cgtggccgcc gccgtcatcc acatcgtcgg gggcgccctg gccgcggccg gcctcccggt
     2161 cctcgtcgtc ggcgccgcgg cggagcgcgc ggccgccctc gtcgtgggct tctccctcta
     2221 cggggcgagc ctcctctact acttcgcgat ctccaccctg gcgcatttcc tcgaagcccc
     2281 cggcgcgcgg cgcgtcttcg cgaccctcga cgaggccgga gacttcttcc tcatcgccgg
     2341 ctcctggacg ccgctctgcc tcacggtctt caagggcccc ctgggctggg cgctcttcgg
     2401 cgtcctctgg ggcctggccc tcttcgattt cctcgtcgcc ctgctctaca cgagccgggc
     2461 caagaacctg gtcctggccg cctactacct gggagtcacc ctggtcctgc ccctcgtcgc
     2521 gcccttccgc ggactcctgg gcgagatcct cttcgcctgg ctcgtcctgg cgggcctcct
     2581 ctacgcggcg ggcctcctct tcaggtcgcg ggacgagatg ccctaccacc acggcatctg
     2641 gcacgccttc accctcgccg cctcgatctg ccacttcttc ggccttctct cccttctttg
     2701 aagccgcctc gcgccccagg gccgccgcgc ccgaggcggc ggtccggagc gagaggcggc
     2761 ggtccggcgc gagaggcccg gcgcgggaga ccgcggcctt gcggcgctag gcgaaggcct
     2821 cggcagcgag gcgggcgagc tcgtcctcgc cctcgttctg gcccgcggcc gcgacggcca
     2881 gctcggcctc ggcctcgccg tccgggcctt cagtggcgag gggcgggctc gcctcgggag
     2941 cctgggcgag gcgcgtcgcg agggcctgga cgccgcgggc gaagggcgag gaggcgtcga
     3001 ggtccaggag tgggcggcgc gccgcgacgc tcttagcgac gcccggatcc tcgaggagga
     3061 agccggagaa ctcgagactg agtcccaggt tcttcctcga gatgtcccgg aggcggtagc
     3121 cgagctcggc atcctccggc gacttgccgc ggttcaggac gacgcgcggc aggaggcggg
     3181 agagggcctc cagggccggc ccgcccctct cgggacgcct ctcggccagt ccccgcgcga
     3241 agtccaggaa ggacaggccc gagccctcgg tcttcgtccc cgcgtaggcc aggacctcgg
     3301 ccctctcctc gctgccttcg gggaaggagc ggtggaggat ccggaaggcg gcggtcttca
     3361 ggaaggagta ggcgttgagg acggaggtta cctcggggca gaggacgagg aggcccgaag
     3421 gcgcggcgac gaagaaatcg acgacgttgt acgacgagcc cgcgcccagg tcgaggagta
     3481 tgaagtccgc ggggagcttc gacagctcct tgaggatcct ccgcttcgag ttccattcga
     3541 ggttggcggt gccggggagg aggttgtcgc cgggcacgag ccagagccgc tcgaagcccg
     3601 tctcgacgag gaggccggag aggctcttct ccgccttcca gacgaaggag cccaggcccg
     3661 cgtgcctgtt ccggaggccc aggcaggtgt ggaggttcga ggcgcccagg tccaggtcga
     3721 cgaggacgac ggtctttccc gcgcgggcca gggcgaggcc caggttggcg gtgaggacgc
     3781 tctttccgac gccgcccttt ccggaggcga tgggcaggat ccgctgcatc aggactccta
     3841 ttcagcgagg actctagcgc ctccggcccc ggatggcaag gccgcccgcg tctcggcggc
     3901 gaaggccgcg cagggggagc ggcgtccgcg gcgcgggcgg aggggcggcg gcggcgctca
     3961 ggtggaccgc cgtcggccgc gttggcgggg cggcggcggc gtgcgggcag aggggcggcg
     4021 cggcgcgcgg gccgcccggg ccgggcgttg ccgaagcccc tgggccgcgc tatggtggag
     4081 gcatggcagc ccccttcccc tccgccctcg cgacccggcc agggcggaag gcccccgctt
     4141 cagccgctcc ggccgcgatc ctcgtcgcgg ccgccttcct cgccctggct ctccccgccg
     4201 ccgccgcgga gagcccgcgc ccctccgtgg agaccgaggc gacggcggcc gaggggctcg
     4261 cgggcctgtc ggtgcgcgcc ttcgcctccg acacgggaga ggagctcaag ggggccttcg
     4321 gcggcgccga gctcagcgtc aacggagccg tggtcgggag gacgccctat tcggacgcgc
     4381 ggatcccgcc gggcacctac cttctcaggg tggaggccga aggctactac ccctccgaga
     4441 tcctcctcgg gctcgcggag aagaccctct acaagctcgt cttccacctc gcgaggatga
     4501 cgggctacct cgccgtcgag gtctcgcccc ccgacgcgga gatcctcgtc gacggcaaga
     4561 gccgcaggcc gggactcatc gagcttccca cgggcggccg caggctggtc gtccgccgct
     4621 tcggctacgc cgagcgcgag tacgcgctat ccatcgagga ccgcgcgacc tccttcctca
     4681 gggtcgagct cgagaaggcc cccttcgcgg tctcggaact ccgctcctcg cgcgcggcct
     4741 tcaatccgcg caacgcgggc ctcctcggaa ggtgcgccct cgacttcgag gtgagctcct
     4801 acggctcggg gaggctctcg atcctcggcc cctcgggaga tgccgtcgcg gccgccgcct
     4861 tcccctcctt cgagacctgg cggcagggct tcgtgtggag aggccgaggg gccgacggcc
     4921 tcccccttcc cgacggagcc tacctcgcgc ggctcgaggc ctggcccgcc gcgccggaag
     4981 gcgcggccgg aagcgcggcc ccgcccctag tccgcgaggt cgccatcggg ctcgactcgc
     5041 gcatcgtcgt cgagcccagg gccgtccagg ccgcctccgc cggactcctc tacttccccg
     5101 atccccgggc cctccccgcg gggagcctcg ccctggagct ctcggcctcc ggcggcgcgg
     5161 acttcagaac tcctccggcc ttcggcctcg gggcctccta ctccctgggc cgggcggggc
     5221 tcgccctcgc cctgacggcc gaaccggggg aatccggagg cgaggccggg gccgagacgg
     5281 ccctgtccct ctccttcgcc ctcgtccgag agcccgggcc cttcgccgcg gccctccacc
     5341 ttcgcgggag ctggtcctcg gccgtagcgc ccatcatggc cggagcctat cccggcggcc
     5401 tcgccgagct cgccctgcca gctgccctct ccttgggcgc cttccgcctg ggcttcgccc
     5461 ccggaatctc ggcggcgatc gcggccgacg gctcggaggc ggcgccccgg cccttcctcc
     5521 gcgcgggagc ctggctctcg ggcagccgct tcctcgctgg cctgtcgggc gagctcgcga
     5581 ccggcggctt gggcaaaagc cccctcgagc ccgactggcc ggcccgcctc gccctggagg
     5641 gccggctcgt cctcgatccc tcgcccctcc tcgtcggggg cgggctgttc ctcgacctgg
     5701 agccaggcgc cgatccggcc ctgcggctcg gcctcgccct cggcctcctc ctctannnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatagc ccgccccgtt ctcctccgcc
     5881 cgccttctct gctatgctct ccgcgagaag cgcgaaggag agaagccatg cgatcgaccg
     5941 tctcgatagt cccttgtccc gagtacgagg ccaaggccct ggaccgcgcc gtcgcggagg
     6001 cagtcgagct cgcgggcggc ttcgaggcga ggggcgcggc cgtcctcctc aagcccaacc
     6061 tcctcaacgc gaacggcccc gagcgcggcg tcaccgtcca tccggctatc gtcgccgcgg
     6121 ccgtgcgctg gctcaaggtc gccggcgcct cccgcgtcct cgtcggcgac tcgccgggct
     6181 ggcagaccca ggagctggtc ggaaggaaga ccggcgtcaa ggacgcagtg ctccagtcgg
     6241 gaggcgagtg ggccgacttc tcgacggagg ccgagttcga gctgcccgag ggccgactgg
     6301 ccaggcgctt ccgcctggcc gctcccctgg ccgaggccga cctcctcgtg agcctcccga
     6361 agctcaagac ccaccagctc atgtactaca cgggcgcggt caagaacctc ttcggcctcg
     6421 tgccggggct ccagaagagc gccttccacc tgcgcttccc cggccgcggc gaattcgcgg
     6481 gcatgctctc cgacctcgcc ctggcctcgg cccccgactt ctcgctcatg gacgcgattg
     6541 tcgccatgga ggggccgggg cccaacaacg gcaggcccag gagcctcggc ctcgtgctcg
     6601 cctcccgcga ccccctggcc ctggactgga cggcggccgg cctcatcggc tacgagccag
     6661 gaaccgtgcc ctacctcgcc gacctcatgg gccgcatggc cgcgaaggcc ggcgacgcgg
     6721 cgcggcctga acgcggcccc gcccccgaga gcgcgcggga gccggcggag gacggagccc
     6781 ggaacggagg ccgggacgag gcgcccgagg cgtcccggag gcgcgccttc ccctcctggg
     6841 ccgcctcgcc cgcggagatc gtcctcgcgg ggctcccgtt cgaggaggcg aagcccgagg
     6901 ccttcgagct cgtccccctc ctcaaggaga acgacttcct ctcgaagaag ctgcccccct
     6961 ggctccaccg cctcgtcaag ggagcgatgg tgccgaggcc gaggttcgac cacgggaagt
     7021 gcgtgcgctg ctcgggttgc gtgcgcatct gcccggccaa ggccctcgtc ctcgccccct
     7081 cggcccgcga cccgcgcggc aggatcgaga tagactacga gcgctgcatc cgctgctact
     7141 gctgccacga gatctgcccc gaggacgcga tcgacctcgt gaggcggtgg ctctgatgga
     7201 ggcgcaagtt ccctgggacg gcctcctcga tttcggcccg agttccgcct accggcgcct
     7261 cgacctgcct cctcccgagc ccttcgccgc ccagcgacgc ggaggcgcgg gcgaaagtct
     7321 cctctacgtc gaggaggcct cgctcgccgc ccttgcccgc gaggccttcg aggagatcgc
     7381 cttcaggctg ccgaccgggc agctcgaggg cttcgccgcc gtcctcggcg accccgaggc
     7441 ctcctccgcc gagcgcttcg tcgccgcgag cctcgtccgc aacgcggccg tcgccgcaga
     7501 gggcgtcctc cccctctgcc aggacacggg ctcggcggtc gcctacgcct ggaagggagc
     7561 cgggatcgcc agctcgggtc gcgacgagga ggctctcgcg gccggcgcgg ccgaggccta
     7621 tgcggcgcgc cggctccgcg cctcggtcct cgccccgacg ggcttcctct ccgagcgcaa
     7681 cacgggctcg aacctccccg ccctcgtcga catcaggcag gccgcgggac atgagtaccg
     7741 cttctgcttc gccgccaagg gcggcggctc gcagaaccgc acgagcctct ccatggagcc
     7801 gccctccctc ctctcggaag cggccctcga gaagaagctc gccgccctcg tcgccgccct
     7861 gggcgcctcg ggttgccccc cctaccggat ctccctcgtc ctgggcggcc ggagccccga
     7921 cgaggccctc tacgccctgg ccctcgcggg gctcggcctc ctggacggac ttcccggaac
     7981 ccccgacgcc tccctggccg acgcgggcgc cggaggcgcg gacctccgcg gaggcccggg
     8041 cgcgggccgc ggggaaggcc ggccccgggg cctcggcctg cccctccgcg accgcggctg
     8101 ggaggatcgc ataacgcgcc tcgcggcggc gacgggcgtc ggggcccagt tcggcggctc
     8161 gaggctcgcc ctggacgccc gggcggtcag gctcccccgc cacgcggcca gcctccccct
     8221 cgccttcggc gtcgcctgcg cggcgcatcg gcgcgcgcgg gccgtcgttc gcgcggacgg
     8281 cttcttcctg gagcgcatgg aggccgaccc ggcccgcttc ctcccccggg agctccccct
     8341 tttcgaaggc gccgccaggg tcgacctgga caggcccctc ggcgagctcg cggccctcct
     8401 cggctccatg aggccaggcg cgcccctcct cctctccggc acggtcgtga ctgcccgcga
     8461 cgccgcccac gcccgtttcc gcgcgctcct cgcggagggc cttcccctcc ccggctacct
     8521 gctccgccat cccgtcttct acgcgggccc cacggaggcg gcgccgggcg aggcctcggg
     8581 ctccttcggc ccgacgacgg cggggcggat ggactcctac ctcgacgcgt tgatggaacg
     8641 gggagcctcc ctcgtcacca tcgcgaaggg cgggcggagc cccgcggccg ccggggcgat
     8701 cgggcggcgc ggcggcgcct acctcgccgt gatcggcggg gcggcggccc tcgcggcccg
     8761 ggagagcgtc gtctcgagcg aggtgatcga ccacgccgac ctgggcatgg aggcggtgag
     8821 gcgcgtcgtc ctcaaggacc ttcccgccat cctcgccgtc gacggctccg ggcgcgactt
     8881 ctacgcggag ctggccgcgg ggggaagcgc ggcggccggg ccgggcgcgg cgaaaggcgg
     8941 agacgcggga gccgggcgat gaggaaggga gctttccgcc tcgggctcgg ctacttctcc
     9001 ttcctcgcgg tcttcggcgt ctccacgccc tacctgcaga tcctcctccg cggcctgggc
     9061 tacggaccgg ccgccgtggg cgtcctcctc ggcgtcttcg agctcgtggg catcgcctcg
     9121 cccttcttcg tcgggaagat agccgactcg aggggacgat ccaggcccgt cctcgcggcc
     9181 tcctgcctcg ccacgctggc cgccctgccc ctcctcgtcc tcgtccgggg cgtcgcggcg
     9241 accgtcgcgg gcctcgtcct cctcgcggtc ggcctcaagg ccctcgcgcc cctcatggac
     9301 gccgtctcca cgaactacgc cgagtcgagg cccgagctcg gcatcaggta cgggaacctg
     9361 cgcgcggcgg gctcggccgg cttcatcctc gtcgccctgg ccctccaggc cctgcccggc
     9421 ttcggcgagg gctcgcctga gcgcatagcc ctggccatgg gcctcctggc ggcggccttc
     9481 ctcgcctacc tgcccctcct gcccgagacg gcgggaggcc cgaggaggcc caagcccggt
     9541 ccggcaggac ggcggggctt cgcccccggc ttcggcctcg gcctcgccgt catagccctg
     9601 agccgcgtcg ccatggcgcc cgtgagctcc ttcttctccc tctacgtgaa ggaggagctg
     9661 cgctggaacg cggtgggcgc gctctgggcc ctgggcgcgg cggcggagat ccccctcatg
     9721 ctcctcgccc accgcatcgt ggccaggctc ggcgcgatgg gcgccatagc agtctccacg
     9781 gccgccgtcg ccctgaggct cgccgtctgc gcggccttcc ccagcccggc gggggcggtc
     9841 gcggcccaac tcctccacgc cctctgctac ggcctcttcc agccggcggc cgtctccttc
     9901 gtcgcccttt ccgccccgcc cgagaggcgg gcgagcgcga tggccctcta catgagcctg
     9961 ggcgtgggcc tccccacctt cctcgggagc gccctcgggg gcctcgtcgt ggagggctcg
    10021 ggctatcgcg tcctcttcgc ctcctacatc cccttcgccc tcgcgtcgat cacgctctgg
    10081 gcggcgggag ggaagcggct cgccgggagc cctagagctc cttccggagg acctcgccct
    10141 tcccgtcgcc gcgcagacgc ctgaagccgg ccctcgcgta gagggccctc gccgcggcgt
    10201 tggaggaaag cacgtagagg cgcatccgcg cgaagccgag ggcgcgtgcc cgggcctccg
    10261 ccccatcgag gagggcggcg ccgaggcctc tgccgcggaa ggcgggcgcc acctcgagat
    10321 ggaagaggaa ggccgtgccg ggctcctcgc cgccggggac ctccccgaac caggcgcggc
    10381 cgacggcctt gccgcccggg gcgaggcggg cgacgaagag gcgttggccg ggcgtcgcct
    10441 cgcccgcggg aaggagctcg tcctgctcgg cggcggcgcg agcggcggcc gcgtcgaggg
    10501 agaggccctg cgactccgcg agggcgcggg ccagctcctc gacgcaggcc ccgcgatagg
    10561 cggcgtagtc gccctctccc atgggctcga ggacgaggga ggccgcggcc gggtcgcgct
    10621 ccgctgacgg tttgcccgcc gccgccggga atcgcgccgc cgccttgcgg cgcgccgctt
    10681 cgggcggccg ccgagccctc acccttcgcc gccctcgagg acgcgctcgg ccatgaggag
    10741 ggccgcggcc attagggcgt ggcggtactc gccggagccc atcagctcgc ggacctcgac
    10801 ggcgggaacg aggaatgcgt cgactatctc gtgctcgtcg agcgcgcggc ggccggtcgg
    10861 ctccaggcgg tcggcgacga agacgtggaa gcggttcccc atgaaggcgg ggttcgggta
    10921 ggtccagcca gcgctccgga tccggcccgc cctccagccg gtctcctctt cgagctcgcg
    10981 cgccgccgcg gcggcgggat cctcgccggg ctcgacgacg ccgccgggga actcgacgga
    11041 ggcctcgtcg gagccgtggc ggtactgccg caccatgagg aaggagggca aggccccgcc
    11101 ctcgcccgcg ggaaggaagg gaacgacggt cgcccagtcg ggggcctcga ggcggacgaa
    11161 gcggccgcgc ctcccgtcgg gcgactcgct ctcgagctcg cccaccgtga agatcggaca
    11221 gtcgaggagc ttccgctccg agagcctctt ccatttcctg ccggccgacc cgtcgcccgc
    11281 gaccatgccc gtcctccctt cgccggcgac ggcgcgccgg cgatgccgct tggacgatga
    11341 tacgccccct cggccccgcg cggctaggcg acggaggccg aggggcgggc aggacgccgg
    11401 ccaggcaggc ggccccgccc agaggggggc cgccgcgtag cgcgcggccg acgcgggctc
    11461 gaggcgtcgc gggcgcgggc gcaaggcggg ctggacgccg gccccccggg ccgcacatac
    11521 tgtcgccaag gatcaaagga gcgagccatg caggattggg cactggaatt ccccggggcg
    11581 gtgacggtct gcgaccggga cttcacgatc atctacatga acgacaagtc ggcgaagacc
    11641 ttcgccaagt ggggcggcga aggcctcctc gggaagagcc tcgtcgattg ccacaagccg
    11701 gagtcgatcg cgacgatgcg ccgcatcctg gagacgggcg agcccaacgt ctacaccatc
    11761 gagaaggaag ggcagaggaa gctcatctac caggctccct ggaggaagga cggcgcgata
    11821 gccggcctcg tcgagctctc cctcgtggtc cccgcggaga tgccgcactt cgtccggagc
    11881 tagggcccag gaaatggcag gggccgccct ctcaggcggc ccccgcggcg agcgccgggc
    11941 ccggtctccc gggcccggcg ggagaaggac tagacggcct tcatccccgc ctcgatggcg
    12001 gccatgttca tcgggatgaa cttgtgcttg gactcgggga agaagctctt gagaacggcc
    12061 tcgagcttgt cgagcttgac ggcgccggtc gccctggaga gggcgcccat catcaccatg
    12121 ttggcgatct tggcgtcgcc gatctgctcg gcgatgtcgt tgcaggcgat cttcaggacc
    12181 ttgaggtcct tgcgcttcgg gtcgaccttg acgaggctcg agttgacgag catgagcccg
    12241 ccctgcttgg cgacctcctc gcagatcggg agcgagtcga tgttcatcac gatggacgag
    12301 tcgggggagg tcacgagggg gctcgcgatc tccttcgtcg acacgatgac ggaggcgcgg
    12361 gcgacgccgc cgcgcttctc cgcgccgtag aaggggaaga aggtgacctc gaggccctcc
    12421 ttcatcgcgc agtagaccca gagctggccc agggagatga tgccctggcc gccgaagccc
    12481 gccatgaagg tcttctcggt catttcgcgg cctcctcgag ggtgttcttg atctcgccga
    12541 gcttgtagta gggcatcatg ttctgctcga gccactcgtt cgcctcgccg ggcttcatct
    12601 gccagttcgt cgggcagggg gagaggatct cgaccagccc gaagccctcg ccgcggagct
    12661 gcgcctcgaa ggccttcttg atgtactgct tggtcttcct gacgttggcg ggggtgttga
    12721 ccgcgccgcg ggccaggtac cgggtgccgc ccagcgtcgc gaggagctcg cagacctgga
    12781 tgggatagcc catgccggcg tcgacgggat cgcggccgta gggggcggtg gtggccttct
    12841 ggccgacgag ggtggtcggg gccatctggc cgccggtcat gccgtagatc gcgttgttca
    12901 cgaagat
//
LOCUS       DAOOSJ010000045        12857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3268_length_12857_cov_5.811904, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000045 DAOOSJ010000000
VERSION     DAOOSJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_12857_cov_5.811904"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLG14_03720"
     CDS             complement(<1..385)
                     /locus_tag="PLG14_03720"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HOX48271.1"
                     /translation="MQTLLDRLVAEGRSLAERGRPASYIPALAKADPGLVGLALADGE
                     GRLLEAGDSRARFTIQSVSKAFALAFALEARGEEAVFSRVGKEPSGDPFNSIIRLETS
                     DRRKPFNPMINAGAIVVASLLPGEGP"
     gene            complement(546..725)
                     /locus_tag="PLG14_03725"
     CDS             complement(546..725)
                     /locus_tag="PLG14_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48272.1"
                     /translation="MPERAPDCLKCLHFRVTWEPRFPRSCEVFGIKSRRLPSLEVFGA
                     TGRHCPAFELKPGLK"
     gene            813..1448
                     /locus_tag="PLG14_03730"
     CDS             813..1448
                     /locus_tag="PLG14_03730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX48273.1"
                     /translation="MDRSSLVRRPFPYSNRNLCLYLIGANILVTAFGYLSPAVPYLLA
                     MNPMSVLSGRIWQPFTYMFVHADLSHLLVNMLGLLFFGTQVERELGSSEFLLYYLLTG
                     FLAGLFSLAAYLLAGAYSVFLLGASGAVFAVLLAYAALFPNSDIYVWGILPVRAPVLV
                     VGYTLIEVVSQLFSFRSSVAHLTHLAGFGFGWLYFLVRFGVNPGRRFFPRR"
     gene            1453..2151
                     /locus_tag="PLG14_03735"
     CDS             1453..2151
                     /locus_tag="PLG14_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48274.1"
                     /translation="MARDSLPRTHSRVALAIGLGLGLGLLAARAASAEPTLRGDFWAE
                     LEPVPQEGEPWPLPVETAAERLVEEAAYVFSGMVSGFEFAYTPLDRARGLEESFKLEP
                     VGVLERGDPRLRPGKARLEAGRLVGPVEFLADASDALELEGYGREPWKSAQGRGYAEL
                     VLGWKGRRVAYEEAAREAARSLLRLLEPNKPRLVRGRLVFASPPRVAIVDGRYAVTAR
                     CRVEVTEVLSYGVY"
     gene            complement(2308..3192)
                     /locus_tag="PLG14_03740"
     CDS             complement(2308..3192)
                     /locus_tag="PLG14_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOX48275.1"
                     /translation="MEPISARYREQILRMMSLSKGQTQVTDRNVHQPGNPELLPFIDK
                     IVEENLLPGSEVRHPERLVELLDAAKRGEPCLLLLEHYSNFDLPVFHYLLRKSGPRGD
                     EAADALIAIAGIKLNESNPAILAFTEAYSRIVIYPSRSLEIIKRNLKDPKELVAEMMR
                     GSTVNRAAMHALAELKTSGNIILVFPSGTRFRPWDPSTKRGVREIDSYVKSFAKMCLV
                     SVNGNILRLNPSGDMAEDLVCQDRVVFDVGPVTDCEAFRDEVKHEHRLRDDKKQAVVD
                     EIMAGLERMHDEVEKARL"
     gene            complement(3255..4919)
                     /locus_tag="PLG14_03745"
     CDS             complement(3255..4919)
                     /locus_tag="PLG14_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48276.1"
                     /translation="MPDEIDPEIAALIGGSVEEYKPGAKPRPAGAPLPPDFESLFGDL
                     GVTAENREKRESDVDFSRKTIAPVEKPEETEPRDLFSDPDYYKKALGGEGDEAQKVHE
                     LLSKYLKATDPKDKGVYRQQLIPSYWYLAAKCALRCASPQVIMPKRLLVRFGALLPSL
                     LSPEIKDVFQRIVFDKLIDEPVYYVDEWIRAVTTGQINPSATDEVKPQRGDDRSHFTA
                     IMQRAQGKRDASEGIMKAKAEERRVLENLLREKVEVVCRHDSQPGLLHVPAPYTEQQK
                     KAMSEFTEIFRRMASADKELVQTMAEFERAGDELKSAGEKAQALGRDAKADFQALAQE
                     FETIKQMSKLCIGRQGNHFPILSREYFHGGIRDVGSRENVVRLLAWVESIDSEAYCRP
                     YKNTMNRIVPYIVLLPCYGDQGICWEPFDRYNRASSRGRVALPMYAKNLQAAVVTAVA
                     DLRWQVAKEKASYYWMEEGLTGNYYQWFTSKKLKGDVKEYFIQDYILWITKESEGTQK
                     LDKEVRAIFWRFLPFTKEIKEKLKTRSYVYQDLYQKDINRSLSDGY"
     gene            5101..5334
                     /locus_tag="PLG14_03750"
     CDS             5101..5334
                     /locus_tag="PLG14_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48277.1"
                     /translation="MPVTVSFVEVDGIALRSAASCRLASGRDPPLALGILPRFRRFAA
                     RGGSFNSVHLNFVWKTKTATVAGDRFFRGGGRN"
     gene            complement(5316..5389)
                     /locus_tag="PLG14_03755"
     tRNA            complement(5316..5389)
                     /locus_tag="PLG14_03755"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5353..5355),aa:Ala,seq:ggc)
     gene            5571..>7372
                     /locus_tag="PLG14_03760"
     CDS             5571..>7372
                     /locus_tag="PLG14_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48278.1"
                     /translation="MKPTTYNAFALCGLGLERVLFMELERLGMEPTGRSPGRVFFAAD
                     AAGLFRANLCLRTAERVLVEAARYRAEDFDALFEGARAPAWEDFFGLEDRLVIERVRA
                     NRSQLDAQTSIQSVVHKAVYEKLGQAYGLERLPETGRERSVRVYLEDDECVIGIDVSG
                     EALHKRGYRKSAGLAPLKETVAAGVLLLAGWSRRLPLLDPFCGSGTLLIEAALFGIDS
                     APGLGRDFELETMPLADPEAFEAERAAAKARIKTDAELFLVGSDVDEAVLAGARANAE
                     RAGVAHLIDFRAGKAEDAEPLAEKGLLITNPPYGERLGTEEEAEDLYRRLGALAPRFK
                     GWGLGFVTNRSDFGDFFGRRAVTERRVMNGAEEQWFHWYPEGFEDRPARGRREEAEPR
                     DQAGRPPRRGKPAPVHAKEKREDSASGPRPRKWEGPDKPGWKPRPAGAGRYRDFEPRD
                     GAERRIAGGFKRHEGGTGPRRENPRRDDERRDQARPEAGRRERYAGSRDEGRSGPPRQ
                     GAPKARWEGPDKPGWKPRPREDARDERRPFAPRRDEPRRDEERRPAYPRRDEARGGDE
                     RRPAYPRRDEARGSDERRPSYPRRDGERKPSYTRR"
     assembly_gap    7373..7472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7473..7784
                     /locus_tag="PLG14_03765"
     CDS             <7473..7784
                     /locus_tag="PLG14_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48279.1"
                     /translation="PRRDDPRRDDDRRPPFARREDTRRDEPRREAYPREGSGEQGKKP
                     KVFRIEPPKRPAPKPVEGSESKPRRGRPPKKDEPASKKSSFFGGSRGPGRIDRNRGED
                     S"
     gene            complement(7854..9356)
                     /locus_tag="PLG14_03770"
     CDS             complement(7854..9356)
                     /locus_tag="PLG14_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOX48280.1"
                     /translation="MGPIDTIGNIPENLDELFPPGLSPDRRARARTLFLKKLSLAAHR
                     QYGGKMQTLPRCALPSRDWLDVWYTPGVSAVSTTIRDAALGRAAAAGGPGAAEDPSFE
                     LSSRGNTVAVVSDSTRVLGDGDCTPSGGLGVMEGKAFLMKYLGGVDAVPLCVDSRLGA
                     AEPDPDNLIRFVRMAQASFGAVNLEDISQPNCFRVLDELQDCEIPVWHDDAQGTAAVT
                     LAGLLNALKLAGKELSEARIVLLGAGAANSTTARFILAAGGDPGRLVLADKEGCLHSG
                     RADLESDPRAYRQWELARSTNPRRLASVAEAVRGADALVALSAPGPGTVKPEWVASMA
                     ERSIVFACANPVPEIWPHAAKEAGAFIVATGRGDFPNQVNNSVCFPGLLKGVLLARAR
                     RVTDAMAIRAARSLASFAERRGIGPESIMPTMDETEVFAIEAAEVAEEAEAEGLARRP
                     IPRAEAYARAKADAEAARSLFRRMQEEGYIPAPDPALVQAALREALAETE"
     gene            9439..10968
                     /gene="zwf"
                     /locus_tag="PLG14_03775"
     CDS             9439..10968
                     /gene="zwf"
                     /locus_tag="PLG14_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOX48281.1"
                     /translation="MTPERLDAASCDLDPDRRPLEPVVVVVFGATGDLTHRKIAPALY
                     NLARSGVLNPESALLGFARRPIPPEAFRAGLREACAGHSHTGLDPALWERFAPRLSYL
                     QGDLDDPAAYARLAALLAESGKPRNALFYLAVAPDQFGLVARRLAAAGLGTAGRDPEE
                     AGPWRRLVVEKPFGCDRHSAHELNRLLQDCFAEKDIFRIDHYLGKETVQNLLYFRFAN
                     SVFEPLWNRRYVERVEIDVFETGGIGTRGGYYDRAGAARDMLQNHLVQLLCLTAMEPP
                     STLDPESIREEKVKVLRSIPAYSLEEYAERSVRGQYLGGRSPEGRDIPDYLSEPKVRP
                     GSRTETYAALRLEVDNWRFAGVPFLLRTGKALDRQASEIRVHFKRPPETLFAGYCRDK
                     LEPNSLVVRIQPEEGLLLAFNSKTPGVAGLKRGDLRFSYRERNTDYFPEAYERLLADA
                     MAGDSTLFIRFDEAEEAWRVIDGLEEAWASPSAPPLVRYPAGASAESRPLAPPEPVAD
                     A"
     gene            11125..11817
                     /gene="pgl"
                     /locus_tag="PLG14_03780"
     CDS             11125..11817
                     /gene="pgl"
                     /locus_tag="PLG14_03780"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOX48282.1"
                     /translation="MRVFSFDDEASWIEAASGELVQAAARARLEGRGDLQLCLAGGAT
                     PAALYAALARPGPAASALRGLAVELWLGDERAVPPEDSERNGALAARAFSGAAWDPEP
                     VLRPWPAGPAAEAAAAYAALLLERLGPRPAFDLAFLGLGADGHTASLFPGQAILGEES
                     ALAAASLAPSPPRERMSLTYPALLGSRRIRFLARGAAKAAVVAALEREEPALPASRLA
                     RSADAAVLYCGV"
     gene            11882..12364
                     /locus_tag="PLG14_03785"
     CDS             11882..12364
                     /locus_tag="PLG14_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48283.1"
                     /translation="MKKTRMLLAAALAAGLVLVPAAFAEEAGPLRRELKELAIQPAEI
                     EAIEKIVEKDAVELAKASGDIKVAQARLERLMLDRPPQMDEIRKLVRSSLELEYAIRM
                     IRIERSIALRQLLGDRRWASLERLVRAYAQARRSGKLKEGDGAEAPAKFLGLLERLEW
                     "
     gene            12358..>12857
                     /locus_tag="PLG14_03790"
     CDS             12358..>12857
                     /locus_tag="PLG14_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX48284.1"
                     /translation="MVIGGEAEELELARRFCRDGDRRAFEELVRLRLPQIRRFLAVRL
                     SEPEEAQAAEQEVLVRLFRSLARFRGESSLSTWIFRLCSASAADLVRQRSRERRRATR
                     LGLSSLEADPPDPGSPGAGSPELGLERKEDAAALRRALAALGEPNASLVYLRDAEGLS
                     VGELAR"
BASE COUNT         1748 a         4555 c         4471 g         1983 t
ORIGIN      
        1 cggggccttc gccgggaagc aggctcgcca cgacgatggc gccggcgttg atcatcggat
       61 tgaagggctt gcgcctgtcc gaggtctcca gcctgatgat cgagttgaag ggatcgcccg
      121 agggctcctt cccgacgcgc gagaagacgg cctcctcgcc cctggcctcc agggcgaagg
      181 cgagggcgaa ggccttggac acgctttgga tcgtgaagcg ggcgcgggag tcgccggcct
      241 cgaggaggcg gccttcgccg tcggcgaggg cgagcccgac gaggccggga tcggccttgg
      301 ccagggcggg aatgtaggag gcggggcggc cccgctcggc gaggctcctc ccttcggcga
      361 cgaggcggtc gagcaacgtt tgcatgcgtg aagtatagcg gccgcgggct ctccgcctca
      421 atacgagggg tggctgaccg agcgtggcgc gcgggcaggg cccggacggc ccgccagagg
      481 gaacggctcc gtgcgagcgg cgggcgcgcc gcgcccgcgg ttccggctcg gcgcgcgccg
      541 gcgggtcatt tcaggccggg cttgagctcg aaggcggggc aatgcctccc cgtcgccccg
      601 aagacctcca gggagggcag gcgccggctc ttgatgccga agacctcgca ggagcgcggg
      661 aaccgcggct cccaggtgac gcggaagtgc aggcatttca ggcagtcggg cgctcgctcg
      721 ggcatcgcgg tatctccgcc tggcatggta gcgcgggcgg cggccttccc gcaagcttgc
      781 cccggggccg aatattccgt acagtagagt ccatggatcg cagcagtctc gtacgcaggc
      841 cttttcccta ctcgaaccgg aacctctgcc tctatctcat cggcgcgaac atcctggtga
      901 cggcctttgg ctacctctct cccgccgtgc cctaccttct cgcgatgaat cccatgtcgg
      961 tgctctcagg caggatctgg cagcccttca cctacatgtt cgtccacgcc gacctctcgc
     1021 acctcctcgt caacatgctc ggcctcctct ttttcggcac ccaggtcgag cgcgagctcg
     1081 gcagctcgga gttcctcctc tactacctcc tcacgggctt cctggccggc ctcttctccc
     1141 tggccgccta cctcctcgcg ggggcctact cggtcttcct cctgggcgcc tccggcgcgg
     1201 tcttcgccgt cctcctcgcc tatgcggccc tcttcccgaa ctcggacatc tacgtctggg
     1261 gcatccttcc cgtgcgggcg ccggtcctcg tcgtcggcta caccctcatc gaggtcgtct
     1321 cccagctctt cagcttcagg agttccgtcg cccacctcac ccacctggcg ggcttcggct
     1381 tcggctggct ctacttcctg gtccgcttcg gcgtcaatcc cggacggcgc ttcttcccga
     1441 ggcgctagag ccgtggcccg agacagcctt ccccggaccc atagccgcgt cgccctcgcc
     1501 ataggcctcg ggctcggcct gggcctcctg gccgcgcgcg cggcgtcggc cgagcccacg
     1561 ctccgcggcg acttctgggc cgagctggaa cccgtcccgc aggagggcga gccctggccc
     1621 cttccggtgg agacggccgc ggagcgcctt gtggaggagg ctgcctacgt cttttccggc
     1681 atggtgtcgg gcttcgagtt cgcctacacg cccctggacc gcgcgagggg cctggaggag
     1741 agcttcaagc tcgagcctgt cggcgtcctc gagcgcggcg acccgcgcct caggcccggg
     1801 aaggccaggc tcgaggcggg gcggctcgtc ggaccggtgg agttcctcgc ggacgcgagc
     1861 gacgccctgg agctcgaggg ctatggccgc gagccctgga agagcgcgca ggggaggggc
     1921 tacgccgagc tcgtcctggg ctggaagggt cggcgggtcg cctacgagga ggccgcgcgg
     1981 gaggcggcga gatccctcct caggctcctc gagccgaaca agccgcgcct cgtgcgcggg
     2041 cgcctcgtct tcgcctcgcc gccgcgcgtc gccatcgtgg acggacgcta cgcggtgacg
     2101 gcgcgctgcc gcgtggaggt gacggaagtc ctctcctacg gcgtctattg agccggcgcc
     2161 tttgtaggag cgggcggcgc ggacggcgcg gacggcgcgg acggcgtggc cgacgcggtg
     2221 ccgccgcggc ttgcggggcc ttgggcgttt aaatgagaag agggcggcca atggccgccc
     2281 tctcgctttg tccgccggcg cctccgtcta gaggcgggcc ttctcgacct cgtcgtgcat
     2341 ccgctccagg ccggccatga tctcgtccac gaccgcctgc ttcttgtcgt cgcggagccg
     2401 gtgctcgtgc ttcacctcgt cgcggaaggc ctcgcagtcg gtgacggggc cgacgtcgaa
     2461 gacgacgcgg tcctggcaga ccaggtcctc cgccatgtcc ccgctcgggt tgaggcggag
     2521 gatgttgccg ttgacggaga cgaggcacat cttggcgaag gacttcacgt aggagtcgat
     2581 ctcccggacg ccgcgcttcg tggagggatc ccagggccgg aagcgcgtgc ccgaggggaa
     2641 gacgaggatg atgttgccgc tggtcttgag ctcggccagg gcgtgcatcg cggcccggtt
     2701 gacggtcgag ccgcgcatca tctcggcgac gagctccttc gggtccttca ggttgcgctt
     2761 gatgatctcg agcgagcggc tcgggtagat gacgatgcgc gagtaggctt ccgtgaaggc
     2821 caggatggcg gggttggact cgttgagctt gatgcccgcg atggcgatga gggcgtcggc
     2881 cgcctcgtcg ccgcggggcc ccgacttccg gagaaggtag tggaagacgg ggaggtcgaa
     2941 gttggagtag tgctcgagga ggaggaggca gggctcgccg cgcttggccg cgtccaggag
     3001 ctcgacgagg cgctcgggat gcctgacctc ggagccgggg aggaggtttt cctcgacgat
     3061 cttgtcgatg aaggggagga gttcggggtt gccgggctgg tggacgttgc gatccgtcac
     3121 ctgggtctgg cccttggaca gggacatcat cctgaggatc tgctctcgat accgcgcgct
     3181 gatgggctcc atgccggtct cctcgagggg cgagtataga cagagtcccc cgggctgtca
     3241 acgtcgggga cttttcagta gccgtcggag agcgagcggt tgatgtcctt ctggtagagg
     3301 tcctggtaga cgtagctccg ggtcttgagc ttctccttga tctccttggt gaaggggagg
     3361 aagcgccaga agatcgcccg gacctccttg tcgagcttct gcgtcccctc gctctccttc
     3421 gtgatccaca ggatgtagtc ctggatgaag tactccttga cgtcgccctt gagcttcttg
     3481 gacgtgaacc actggtagta gttgccggtg aggccctcct ccatccagta ataggaggcc
     3541 ttctccttgg cgacctgcca gcgcaggtcc gcgaccgcgg tgacgaccgc ggcctggagg
     3601 ttcttcgcgt acatgggcag ggccacgcgg ccccggctgg aggcgcggtt gtagcggtcg
     3661 aagggctccc agcagatgcc ctggtccccg tagcagggga gcaggacgat gtagggcacg
     3721 atccggttca tcgtgttctt gtagggccgg cagtaggcct cgctgtcgat gctctccacc
     3781 caggcaagga ggcggacgac gttctccctc gagcccacgt cgcggatgcc cccgtggaag
     3841 tactcacggg agaggatggg aaagtggttg ccctgcctgc cgatgcagag cttggacatc
     3901 tgcttgatcg tctcgaactc ctgggccagg gcctggaagt ccgccttggc gtcgcggccc
     3961 agggcctggg ccttctcgcc cgcggacttg agctcgtcgc ccgcccgctc gaattcggcc
     4021 atcgtctgga cgagctcctt gtcggccgac gccatccgcc ggaagatctc cgtgaattcg
     4081 gacatcgcct tcttctgctg ctcggtgtag ggcgcgggga cgtggaggag gccgggctgg
     4141 ctgtcgtgcc tgcagacgac ctccaccttc tccctgagga ggttctcaag gacgcggcgc
     4201 tcctcggcct tggccttcat gatgccctcc gaggcgtcgc gcttgccctg ggcccgctgc
     4261 atgatggcgg tgaaatggct gcggtcgtct ccgcgttggg gcttcacctc gtcggtcgcg
     4321 ctcgggttga tctggccggt cgtgacggcc ctgatccact cgtccacgta gtagacgggc
     4381 tcgtcgatga gcttgtcgaa gacgatcctc tggaagacgt ccttgatctc cggggagagg
     4441 aggctgggga ggagggcgcc gaagcgcacg aggagccgct tgggcatgat gacctgcggc
     4501 gaggcgcaac ggagcgcgca cttcgcggcg aggtaccagt aggaggggat gagctgctgg
     4561 cggtagacgc ccttgtcctt ggggtccgtc gccttcaggt acttggagag gagctcgtgg
     4621 accttctgcg cctcgtcgcc ctcgccgccc agggccttct tgtagtagtc cgggtcggag
     4681 aagaggtccc ggggctcggt ctcctcgggc ttctccaccg gggcgatggt cttgcggctg
     4741 aagtcgacgt cggactcgcg cttctcccgg ttctcggccg tgacgcccag gtcgccgaag
     4801 aggctctcga agtccggggg gaggggcgct cccgccggcc tgggcttggc tccgggcttg
     4861 tattcctcga cgctgccgcc gatgagggcc gctatctcag gatcgatctc gtcgggcacg
     4921 gggacgctcc gcttgggcca ggtgctcggc gtcccgcgcc atccttgccg ggatgggctt
     4981 cgagcgccgg cgatacgatc attctaccgc ctcggattgc cgccttcaag ggcgaaggtc
     5041 cctccgtcgc gccaaggcgc gcccttcaat tccgtccacc taaaataaaa acggccaccg
     5101 ttgccggtga ccgtttcttt cgtggaggtg gacggaattg ccctccgctc ggcggcgtcc
     5161 tgccgcctcg cttcgggccg cgatccgccg ctggcgctcg gcatcctgcc tcgcttccgc
     5221 cgcttcgcgg cgcgcggcgg atcgttcaat tccgtccacc tcaacttcgt ctggaaaaca
     5281 aaaacggcca ccgttgccgg tgaccgtttc tttcgtggag gtggacggaa ttgaaccgtc
     5341 gacctcttga atgccattca agcgctctag ccaactgagc tacacccccg aaagcaagag
     5401 catcttactt acaatggatt ggagtgtcaa tacgaggcga gaaatcggaa gcgttttttg
     5461 cgcaagatca gcgcggagct ggcgttggcc ttggacgggg cttgcgggcc gcggcgaagc
     5521 tccgcatgcg ccccttgtgc ctcgaaggct tcgcggtaca tactattagg gtgaaaccca
     5581 cgacgtataa tgcattcgct ctctgcggcc tgggcctgga gcgcgtactt ttcatggagc
     5641 tcgagaggct cggcatggag cccacgggac gctccccggg gcgggtcttc ttcgccgccg
     5701 acgcggccgg cctcttccgg gccaacctgt gcctgaggac cgccgagcgc gtcctcgtcg
     5761 aggcggcgcg ctaccgggcc gaggacttcg acgccctctt cgagggcgcc cgcgcccccg
     5821 cctgggagga tttcttcggc ctggaggacc gcctggtcat cgagcgcgtc cgggccaacc
     5881 gctcccagct cgacgcccag acctccatcc agtccgtcgt ccacaaggcg gtctacgaga
     5941 agctcggcca ggcctacggc ctcgagcgcc tgcccgagac tggccgagag cgctcggtcc
     6001 gcgtctacct cgaggacgac gagtgcgtca tcggcatcga cgtctccggc gaggccctcc
     6061 acaagcgcgg ctaccgcaag agcgccggcc tcgcccccct gaaggagacg gtcgccgccg
     6121 gcgtcctcct cctcgcgggc tggagccggc gccttcccct cctcgacccc ttctgcggct
     6181 cgggcaccct cctcatcgag gcggccctct tcggcatcga ctcggcgccg gggctcggcc
     6241 gcgacttcga gctcgagacc atgcccctcg ccgatcccga ggccttcgag gccgagcggg
     6301 ccgcggcgaa ggcgaggatc aagaccgacg cggagctctt cctcgtgggc agcgacgtcg
     6361 acgaggccgt cctggccggc gcgcgggcca acgccgagcg cgcgggcgtc gcccatctca
     6421 tcgacttccg cgcgggcaag gccgaggacg ccgagcccct cgccgagaag ggcctcctca
     6481 tcaccaatcc gccctatggc gagcgcctgg gcacggagga ggaggcggag gatctctacc
     6541 gccgcctcgg cgccctggct ccccgcttca agggatgggg cctcggcttc gtcaccaacc
     6601 gctccgactt cggcgacttc ttcggccgcc gcgccgtcac ggagcggcgc gtcatgaacg
     6661 gcgccgagga gcagtggttc cactggtatc ccgaaggctt cgaggacagg ccggcccgcg
     6721 gccgccgcga ggaggccgag ccgcgcgacc aggctggaag gccgccgcgc cgcggcaagc
     6781 ctgcgcctgt ccatgccaag gagaagcgcg aggactcggc ctccgggccc aggccgcgca
     6841 agtgggaagg ccccgacaag ccgggctgga agccccggcc tgccggcgcc gggcggtatc
     6901 gcgatttcga gccccgggac ggcgccgagc gccgcatcgc cggcggcttc aagcgccacg
     6961 agggcgggac gggcccgcgc cgcgagaacc cgcgccgcga cgacgagcgc cgtgaccagg
     7021 cgaggcccga ggcggggcgc cgcgagcgct acgccggctc cagggacgag ggccggtccg
     7081 gcccgcctcg gcaaggcgcc ccgaaggccc gctgggaagg tcccgacaag ccgggctgga
     7141 agccccggcc gcgggaggat gcccgcgacg agcgcaggcc cttcgcgccg cgccgcgatg
     7201 agccgcgccg cgacgaggag cgcaggcccg cctacccgag gcgcgacgaa gcgcgcggcg
     7261 gcgacgagcg caggcccgcc tacccgaggc gcgacgaagc gcgcggcagc gacgagcggc
     7321 gcccgagcta cccgcgccgc gacggggagc gcaagccttc ctatacgaga cgnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgcgccg cgacgatccg cgtcgcgacg
     7501 acgatcgccg ccctcccttc gcgcggcgcg aggatacgcg gcgcgacgag cctcggcgcg
     7561 aggcctatcc ccgcgagggc tcgggcgagc agggcaagaa gcccaaggtc ttcaggatcg
     7621 agccgcccaa gcggcccgcg cccaagcccg ttgagggctc agagtcgaag ccccggcgcg
     7681 gccgccctcc caagaaggac gagccagcct cgaagaagtc gagcttcttc ggcgggagcc
     7741 gcggtcccgg ccggatcgac cgcaaccggg gcgaggacag ctagaacgac gagaggggcg
     7801 cctgagccgg gcgcccctct ctttttcatg gtcggatctc ctgtccgggg cgcctactcg
     7861 gtctccgcca aggcctcgcg gagcgcggcc tggaccaggg ccggatccgg cgcggggatg
     7921 tagccctcct cctgcatgcg cctgaagagc gagcgggccg cctcggcgtc ggccttggcc
     7981 cgcgcgtagg cctccgcgcg ggggatgggc ctcctcgcca ggccctcggc ctcggcctcc
     8041 tccgccacct ccgccgcctc gatcgcgaag acctcggtct cgtccatcgt cggcatgatg
     8101 ctctcggggc cgatgccgcg gcgctccgcg aaggaagcga ggctcctcgc ggccctgatg
     8161 gccatcgcgt ccgtgacgcg cctggcccgc gcgaggagga cgcccttcag gaggccgggg
     8221 aagcagaccg agttgttcac ctggttcggg aagtcgccgc ggcccgtcgc gacgatgaag
     8281 gcgcccgcct ccttcgccgc gtggggccag atctcgggaa cggggttggc gcaggcgaag
     8341 acgatggagc gttcggccat cgaggcgacc cattcgggct tcacggtgcc cggccccggg
     8401 gcggagaggg cgacgagggc gtcggccccc ctgacggcct cggcgacgga ggcgaggcgg
     8461 cgggggttcg tggagcgggc gagctcccac tggcggtagg cgcgcgggtc ggactcgagg
     8521 tcggcgcggc cggagtgcag gcagccctcc ttgtcggcca ggacgaggcg cccggggtcg
     8581 ccgcccgcgg cgaggatgaa gcgggccgtc gtcgagttcg cggctcccgc gcccaggagg
     8641 acgatcctcg cttccgaaag ctccttgccc gcgagcttga gggcgttgag gaggccggcc
     8701 agggtgacgg ccgccgtgcc ctgggcgtcg tcgtgccaga cggggatctc gcagtcctgg
     8761 agctcgtcca ggacgcggaa gcagttgggc tgggagatgt cctcgaggtt caccgcgccg
     8821 aaggaggcct gggccatcct cacgaagcgg atgaggttgt cggggtcggg ctcggccgca
     8881 ccgaggcggc tgtcgacgca gagcggcacg gcatcgacgc cgcccaggta cttcatgagg
     8941 aaggccttgc cctccatcac cccgaggccg cctgagggcg tgcagtcccc gtcgccaagg
     9001 acccgggtcg agtcgctcac gacggcgacg gtgttgccgc gggaggagag ctcgaaggag
     9061 ggatcctcgg ccgcgccggg gccgcccgcc gccgcagcgc ggcccagcgc cgcgtcgcgg
     9121 atcgtcgtgg agacggccga cacgccgggc gtgtaccaga cgtccagcca gtcccgcgag
     9181 ggcagggcgc agcgggggag ggtctgcatc ttccccccgt actggcggtg ggcggccagg
     9241 gagagcttct tcaagaagag ggtccgcgcc ctggccctcc tgtcggggga gagtcccgga
     9301 ggaaagagct cgtcgaggtt ttccggaatg ttgccgatag tatcgatggg gcccatggac
     9361 tcacctccgc gcgatcttcc gacctttcgc ggagtatagc gcggcgtccg cgggcgccgg
     9421 aagggagcgg gacaggatat gacgccggaa cgcctcgatg ccgcctcctg cgatctcgat
     9481 cccgaccgcc gtcccctgga gcccgtcgtc gtcgtcgtct tcggcgcgac cggcgacctt
     9541 acccaccgca agatcgcgcc cgcgctctac aacctagcca ggagcggcgt cctcaatccc
     9601 gaaagcgccc tcctcggctt cgcgcggcgc cccatcccgc cggaggcttt ccgcgccgga
     9661 ctccgcgagg cctgcgctgg ccactcccac acgggactcg acccggccct ctgggagcgc
     9721 ttcgcgccgc gcctgtccta cctccaaggc gacctggacg atcccgccgc ctacgcaagg
     9781 ctcgccgccc tcctcgccga gtccggcaag ccgaggaacg ccctcttcta cctcgccgtc
     9841 gcccccgacc agttcggcct ggtggccagg cgcctcgcgg cggcgggcct gggcacggcc
     9901 ggccgcgatc ccgaggaggc cggcccctgg cgccgcctcg tcgtggagaa gcccttcggc
     9961 tgcgaccggc acagcgccca cgagctcaac cgcctcctcc aggactgctt cgccgagaag
    10021 gacatcttcc ggatcgacca ctacctcggg aaggagacgg tccagaacct cctctacttc
    10081 cgcttcgcga actccgtctt cgagcccctc tggaaccggc gctacgtgga gcgcgtggag
    10141 atcgacgtct tcgagaccgg cggcatcggg acgaggggcg gctactacga ccgggccggc
    10201 gcggcccgcg acatgctcca gaaccacctg gtccagctcc tctgcctcac cgcgatggag
    10261 ccgccctcga ccctggatcc cgagtccatc cgcgaggaga aggtgaaggt cctgcgctcc
    10321 atccccgcct acagcctcga ggagtacgcc gagcgctcgg tgcgcggcca gtacctgggc
    10381 gggaggagtc ccgagggccg ggacatcccc gactacctct cggagcccaa ggtgcgcccg
    10441 ggctcccgca cggagaccta cgccgccctg cggctcgagg tggacaactg gcgcttcgcc
    10501 ggcgtgccct tcctcctcag gacgggcaag gccctggacc gccaggcaag cgagatccgc
    10561 gtccacttca agcgcccgcc cgagaccctc ttcgcgggct attgccgcga caagctcgag
    10621 ccgaacagcc tcgtcgtccg catccagccc gaggaggggc tcctcctcgc cttcaactcg
    10681 aagacccccg gcgtggcggg cctgaagcgc ggcgacctgc gcttctccta ccgcgagcgg
    10741 aacacggact atttccccga agcctacgag cgcctcctcg ccgacgcgat ggccggcgat
    10801 tcgaccctct tcatccgctt cgacgaggcc gaggaggcct ggcgcgtcat cgacggcctg
    10861 gaggaggcct gggcctctcc ctcggctccg cccctcgtcc gctatccggc cggcgcctcc
    10921 gccgagagcc ggcccctcgc gccgcctgag cccgtcgcgg acgcctagct tcgcccctcg
    10981 tggacgccta gcttcgcggc ccctgacgcc gccgcaaagc cgtggccgcg tgcggcgcgg
    11041 ccgccatgcc ccgccgcccc tgacgccgca gccttatccc cgccgcccct gacgccgccg
    11101 cagccctggg cctatactcg acgcatgcga gtcttctcct tcgacgacga ggcctcctgg
    11161 atcgaggccg cctccggcga gcttgtccag gccgccgccc gcgcccgcct tgaggggcgc
    11221 ggcgacctcc agctctgcct cgcgggcggc gcgacgcctg cggccctcta cgccgcgctg
    11281 gcccggcccg gacccgcggc cagcgccctc cggggccttg cggtcgagct ctggctcggc
    11341 gacgagaggg ccgtcccgcc ggaggattcc gagcggaacg gcgccctggc cgcgagggcc
    11401 ttttccggcg cggcctggga tcccgagccc gtcctgcggc cctggccggc gggcccggcg
    11461 gcggaggccg cggccgccta cgcggccctc ctcctcgagc gcctcggccc ccgtcccgcc
    11521 ttcgacctcg ccttcctcgg cctgggcgcg gacggccaca ccgcgagcct cttcccaggc
    11581 caggccatcc ttggcgagga gtcggcgctc gccgccgcct ctctcgcgcc ctcgcccccc
    11641 cgcgagcgca tgagcctcac ctatccggcc ctcctcggct ccaggaggat acgcttcctc
    11701 gccaggggcg cggcgaaggc cgccgtggtc gccgccctgg agcgggagga gccggccctg
    11761 ccggcctcgc gcctcgcccg ctccgccgac gcagccgtcc tatattgcgg ggtctaggcg
    11821 cttttcgcgg aaccgcgggc gccgtcgcgg cgactaacgt aacgcaacgg aggtggtgaa
    11881 gatgaagaag acccgcatgc tcctcgccgc ggccctggcc gccggcctag tcctggttcc
    11941 cgcagccttc gccgaggagg ccggccccct gcgccgcgag ctcaaggagc tcgccatcca
    12001 gcccgcggag atcgaggcca tcgagaagat cgtcgagaag gacgccgtgg agctcgccaa
    12061 ggcgtcgggc gacatcaagg tcgcgcaggc ccgcctggag cggctcatgc tggaccggcc
    12121 gccccagatg gacgagatca ggaagctcgt gcgctcgagc ctggagctgg agtacgcgat
    12181 caggatgatc cgcatcgagc ggagcatcgc cctccgtcag ctcctgggcg accggcgctg
    12241 ggcgagcctg gagcggctgg tcagggccta cgcccaggcg cggcgctcgg gcaagctcaa
    12301 ggaaggcgac ggggccgagg cgcccgccaa gttcctgggc ctcctggagc gcctggagtg
    12361 gtgattggcg gagaggcgga agagctcgag ctcgcgaggc gattctgccg cgatggcgac
    12421 aggcgcgcct tcgaggagct cgtccgcctc cgccttcccc agatcaggcg cttcctcgcg
    12481 gtgcgcctct ccgagcccga ggaggctcag gcggccgagc aggaggtcct cgtgcggctc
    12541 ttccgctccc tggcccgctt ccgcggcgag tcgagcctct caacctggat cttccggctc
    12601 tgctccgcct cggccgccga cctggtccga cagaggtcga gggagaggcg gcgggctacg
    12661 cggctcggcc tctcttccct cgaggccgat ccgcccgacc ccggaagccc gggcgcggga
    12721 agtcccgagc tcgggctcga gcgcaaggag gacgcagcgg cgctgcgccg cgcgctcgcc
    12781 gccctgggcg agccgaacgc ctccctggtc tacctgcgcg acgccgaggg gctctccgtc
    12841 ggcgagctcg cgcgctg
//
LOCUS       DAOOSJ010000046        12582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3351_length_12582_cov_4.926239, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000046 DAOOSJ010000000
VERSION     DAOOSJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_12582_cov_4.926239"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="PLG14_03795"
     CDS             complement(<1..>1406)
                     /locus_tag="PLG14_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48285.1"
                     /translation="LSYSDPEFELGPELKIGRETYALFSAPSLKADASAALQDGSYAS
                     GGLSLGAVLGPLGLKASMRGSASAEGAALRGGHEAALPLGPLALADRFDFDPEDGSFG
                     KEVGLSLKAGKALGLALNGTTLWSPSAASLGSLAQSWGLKLDAGAGRFLGELKGASAA
                     RPSSPIDLDAKYFGAWAESFAYLLPAAEAEADSRSASLSLSGRLDAEREILSAMASFS
                     GLPQAAGGALRDDAAKLRIQAPLKLKSGLALAPYYQRAWSERRGGAGSGILEGSALAL
                     GDLASAEPLWRGIPFAELFDAASVSTFGAYTGPDGAGLSKATYKPEAGVILSREYGSA
                     WWDLLAPSALSLSYARELIRADSSLTSTQVLEWSAKSGAINLFGAFGSYPATSLFESD
                     EYFQAVQGRFAKVEGESAIRKKLILQELATFYAKGNLDSLTVESRFSYSEEPALTEWS
                     ERLNLTLSVTRSRHWLLDL"
     assembly_gap    1407..1506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1507..3299)
                     /locus_tag="PLG14_03800"
     CDS             complement(<1507..3299)
                     /locus_tag="PLG14_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48286.1"
                     /translation="MVKRSASRAAALLLGSVLASAFPLGVAGQVSGQAEDPATVLSAG
                     AFVPRTLPTEAPKSLFAVDLDGEGGEADAELDIRGSWEASLLAEASLEAGGGGLALSE
                     AQPLLFTQRPDLFLSFLLFKKVFVEARVSEDLAEARYSFGYRGGEGEALKEVRAGNDG
                     ISFPALPFLSFGSGSRRSFGAAAKVETEAFSGSAMLRYDQADRVEKRFVGRSEVTETV
                     VAASGFQRGRYFATTSGAAANLVLYVESSAGTFQGSDGKAYRRLEAGEYSYSAATGFI
                     ALAAAATTRVAASYDGSAGTLTVGGQTGCVLLFEPEVFGANTEVLCRYPVLGASSAEV
                     FVRDRASGLRDDDYEARFDSGGFIEVTKGGQTVPTHADYRQPFLSEMADLYTTDFSED
                     DPDILAALEAPLLTKEIVVRRLAAAAEITIDKDVIEGSVEVRRNGVPDYAFTVDADSG
                     LLKLASEPGLEEEILVSYLRESSERRAGSLAAGLAGVFDLGEERAAWAALGLRWSVPG
                     QSYAEGGTSNPGSVVLTAGERDGEGRFRHKAALAGSWTTEEASGRYRVEGMAGAGSYA
                     TSFGVVSALPAGTEVAELEEADLEGLFPAYIG"
     gene            3365..4669
                     /locus_tag="PLG14_03805"
     CDS             3365..4669
                     /locus_tag="PLG14_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48287.1"
                     /translation="METRNDPLARLLRVALDILIVLAVALVFAEEAAAGMGAAWTLRR
                     VLALASAAFDLFFAAEFSARLALAVSDGEAGLYLVEKGGWLDGASSLPILVFVSGPFL
                     FGTLAGVHPAVSVVGSMGAIRILRSLRLLRSLRLLRFLRASGGGSGLAARRHLEKAAG
                     LALGAAVAAALLIEAASALGAWPDARSVLEDKRRATVATLAGAPGADGAEAAAAVDAD
                     LLLVRENGKIVYTRYSSEEYRRRFGPDEIGYLADQGGTEAFFSISAENRARAASALAS
                     ALAALAAFLAVILGYGPYFAAGVSKPVNASLALLEGGGDRSGLFPPPGRDEEEPFRLS
                     LALAEYLDRQRGIDFEAPPLFDEAELPASAQQASSPAHESAPAMEPPRAASNAVTAAD
                     PAPRPASLFAAAARFRSGQGAAPAGEEELEALLDPEAGREGP"
     gene            4666..4959
                     /locus_tag="PLG14_03810"
     CDS             4666..4959
                     /locus_tag="PLG14_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOX48288.1"
                     /translation="MMVVSMIQIIIEVPEAATIKDKRRIVTMTKERLRTKFRISCAEV
                     DLLDSLRFAQLGGAIVSNSREFGEKVMQKALSFVEDGLALKVHDAQIHSELYD"
     gene            4971..5603
                     /locus_tag="PLG14_03815"
     CDS             4971..5603
                     /locus_tag="PLG14_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48289.1"
                     /translation="MLDPMSRRRGALAAAVLASAVLLASCVGVDAEATVARDGSGRLK
                     LRYAVSKMLVSLGALEANERLLPFPVSREDFERTVRGIPGLGLVSYAQKETEEDLVVD
                     AELSFATPQALAAFLDPKGERAAYSESGGEKRLRLVLAEGQAKLDPDLDKLVRAAFAP
                     YSVSLSVKLPSAAKSAGIGKSARGGLDLSYSSPVVDLVTSGSPVVWEIRW"
     gene            5630..6868
                     /locus_tag="PLG14_03820"
     CDS             5630..6868
                     /locus_tag="PLG14_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48290.1"
                     /translation="MTKMTTRRFALLRGLSFASALAAALLFVACATAPALAGPRPAPG
                     GFAPAGSAADAPAKAAPGAPAVMAAGPSAAETEASSLMDKARAAIDSNHLAEGIRSYV
                     SVIAIHEESPSPATKALADAAASELARIGARLSIEPGSEWVDAKGGQLAGASRAVGKE
                     GALSPSVYLYENFGAGKSPVADAPIYFEFARNSGSVVSLVTTDAYGKANTTIARLDEP
                     GKEALVRAYPLFRARGRTYAFKSVFRDFSFLPPANVARIVALETGEFGATDNPQVVDA
                     LASVLKPLGLQFVPYNGKLAQDSFRRAFGGDRAALSSFGAVAEEPYAAFVLVEVSPTR
                     QMELNGKKYNIFTAEAAATFRLVRSDGTIVFALPLDRLKGQGGTPEAAVQDALRRARE
                     ALSPELEKRLPAIKAALEKE"
     gene            complement(6843..8039)
                     /gene="dinB"
                     /locus_tag="PLG14_03825"
     CDS             complement(6843..8039)
                     /gene="dinB"
                     /locus_tag="PLG14_03825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOX48291.1"
                     /translation="MGDVVFHVDLDAFFASVEQLDRPELRGRPVVVGAAPGRRGVVST
                     CSYEARAYGIHSAMPISEAWRRCPGAAFLPVRMERYAEISERVMEAFGEFSPEVRRVS
                     IDEAFLGMSGTERLWGPPEAAAAALKARVRERTGLSISVGVAANHYVAKIASGLRKPD
                     GLVVVEPGGEAAFILALPLGKLWGAGEKTQERFAELGIRTTAQLAAMGERSLASLFGG
                     SGGRFLYEAVRGRDPGIAPREAESRSVSAERTFELDVSDRSLLETLLVELADQLAYRL
                     WSEDLRSRRVFIKLRFQDFSTLTRRRSRPEPFASAEEARAAALALLDSAWDGRRAVRL
                     LGLGFAELEPRGTPLQGELFDGGSARKRAAEEAVFEIERRGLGSLRRARLVDRGGDPT
                     PSREPP"
     gene            complement(8049..10454)
                     /locus_tag="PLG14_03830"
     CDS             complement(8049..10454)
                     /locus_tag="PLG14_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48292.1"
                     /translation="MKIPPLAGLALAVFLLGGAAPAGAESPLSVPTPGPASAVLATPG
                     PASAATAVPGASGSGAAPGPASGSGAAPGAAPGASPAAASSLGSDRAALHLAALASFG
                     PRRENSEAERKAAAYLRAACAAAGARLRDYDLARPGAGHSYSSLFEVGFEGTRPDRLV
                     FALPLGAFEDGEPGQGDAGLALALAEIERLAAPGRGELPIGVSFLFLGAERRGRASEP
                     GSLGYGSESWIAGKEGDPPLAVVYLDFDAMPSAIGLLNSGGGLLSPYWHYERTRAAAA
                     ASGFPYRLFANRMQVSRLGLSDAPSHISPYLLAGIPAVELRGEEGPPASPGAARAAFS
                     RLVDGLVAGNAEGFLETWDRHYLSFQLGRASFVLREKAYVSLLLAFCAGVAALLAAFT
                     LARRDAAKALARRVPSMLGQFLVLFAATTAVYALCRLAAGLESLVLGSKEGWRLAPRA
                     FAGARLLGSLLLCLAAISLLVDRRLLTSNPYFYEFASLVCLGLDVFAFSAMSPPLAFY
                     FAWAFLVVGASLAARRPWTSLAAYALMYAPFLLLVADLAVAPEYGLYERLVSPSLPGA
                     LLLAAAALPFNAFTASPLLFFRPHTAKGKKAEALLFALLALAVEGSALGYAVLSPSRN
                     LAALTLSESVDQDKGSFEARLEGRRRIGRVELDRGGEALELSSGGDSAAFVGAEGSAW
                     VSIRAERRSFLGRSLFRTTLDFAKPPNSLRLRLEGGEDLRIYDCSLPYRVSIDGRRAE
                     IFVGDNPPDPLVVELAAPRGFSAAFVAEASYLEPLVPYGEARGADVVPGRFGLRASAE
                     LGD"
     gene            10569..11729
                     /locus_tag="PLG14_03835"
     CDS             10569..11729
                     /locus_tag="PLG14_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48293.1"
                     /translation="MQERKSVLDLPIKVVLTEEGTSFFIRKGKSLKKFKLADKVEEYG
                     IVLDSFSPASLQRMMLVDYISKVEISKGEFMSSRQEIMDLSKLVVFSVLYRQYDSYIF
                     ARVLASDVIKKWNRLNPANIIDEKTRINDGFLQTVLKEKDKEIREIKEQILAPMCAYI
                     SKNSSLLPEEKNIQLLLSEKFLNNLRPFTWFIIAKFKGADGYDQLLKDIRTSLAEYME
                     KSKIAEYVSLMIMELAMNAENSNLKREAKAIFKGAVDMNAVLFDPNIRRQVIDSLERR
                     GELVYLAWKIGSRNASIGTQGRLQITLFNKESEYQNMKEAFDEKKNADLKKKSLQDFY
                     KQLPGGEANAELGLYYLSYLSEACEKVNVKFESLVNQLPGSDLTVMTLVLNL"
     gene            11939..>12582
                     /locus_tag="PLG14_03840"
     CDS             11939..>12582
                     /locus_tag="PLG14_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOX48294.1"
                     /translation="MAKTKYVYAFGGGVAEGAGISKDILGGKGAGLMEMTKIGLPVPA
                     GFTIGIPACEEYYKAGRKLPKGIVDEVKLAVAKLEKTAGKKLGDSKDPLLVSVRSGAA
                     RSMPGMLETILNLGLNDKSVEGLAARTANPRFAYDSYRRFIQMYATTAMGLSKEPMEE
                     MLADMKKKLGVKNDTDLSADNLKKLCEEFKDFYKQKKGQAFPQDPMLQLWGAIDA"
BASE COUNT         1972 a         4460 c         4304 g         1746 t
ORIGIN      
        1 aggtcgagga gccaatgccg gctcctcgtg acggagagcg tcaggttgag ccgctcgctc
       61 cactccgtca gggccggctc ctcggagtag gagaagcggc tttccacggt gaggctgtcg
      121 aggttgccct tcgcgtagaa ggtggcgagc tcctggagga tgagcttctt gcggatggcg
      181 gactcgccct cgaccttggc gaagcggccc tggacggcct ggaagtactc gtccgactcg
      241 aaaaggctcg tggcgggata ggacccgaag gcgccgaaga ggttgatggc ccccgacttc
      301 gcgctccact cgaggacctg ggtcgaggtc aggctcgagt cggcacggat gagctccctg
      361 gcgtaggaga gggacagggc cgagggggcc aggaggtccc accaggccga gccgtactcc
      421 cgcgagagga tgacgccggc ctcgggcttg taggtcgcct tggagagacc ggctccgtcc
      481 ggtcccgtgt aggctccgaa ggtcgaaacg ctcgcggcgt cgaagagctc ggcgaagggg
      541 atgccccgcc agagcggctc ggccgaggcg aggtctccga gggcgagggc gctcccttcg
      601 aggatgcccg agccggcccc gccgcgccgc tcgctccagg cccgctggta gtagggcgcg
      661 agcgcgaggc cgcttttcag tttcaggggc gcctggatcc gcagcttggc cgcgtcgtcg
      721 cgcagggctc ccccggcggc ctggggcagg cccgagaatg aggccatggc cgagaggatc
      781 tcccgctcgg cgtcgagcct tcctgagagc gacagcgacg cggagcggct gtcggcctcg
      841 gcctccgcgg cggggagaag gtaggcgaag gactcggccc aggcgccgaa gtacttcgcg
      901 tcgaggtcga tcggggacga gggcctcgcg gcgctcgccc ccttgagttc gccgaggaag
      961 cggccggcgc ctgcgtcgag cttgaggccc caggattggg cgagggagcc caaggaggcg
     1021 gccgaggggc tccagagcgt cgttccgttc agggcaagtc ccagggcctt cccggccttc
     1081 agcgagagcc cgacctcctt cccgaaggag ccgtcttcgg gatcgaagtc gaagcggtcc
     1141 gcgagggcga gggggccgag aggcagggcc gcctcgtggc cgcctctgag ggccgcgcct
     1201 tccgcgctcg ccgagccacg catcgaggcc ttgaggccca aggggcccag caccgcgccc
     1261 aggctcaagc cccccgaggc gtagctgccg tcctggaggg cggccgaggc gtcggccttc
     1321 agtgaaggcg cgctgaagag ggcgtaggtt tcccttccta ttttgagctc ggggccgagc
     1381 tcgaactcgg gatcagagta ggagagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnccaa tgtaagccgg aaagagtcct tcgaggtcgg cctcctcgag ctcggcgacc
     1561 tcggtccccg caggaagagc actgaccacg ccaaacgagg tcgcataact tcctgcgccc
     1621 gccatgccct cgacgcggta gcgcccggag gcctcctccg tcgtccagga gcccgcgagg
     1681 gcggccttgt gcctgaagcg tccctcgccg tcccgctcgc cggccgtgag gacgacgctg
     1741 cccgggttgg aggtcccgcc ttcggcgtag ctctggccgg ggacgctcca ccgcaagccc
     1801 agggctgccc aggcggctcg ctcctccccc aggtcgaaga cgccagcgag gcccgcggcc
     1861 aggcttcccg cccggcgctc ggagctctcg cggagatacg agacgaggat ctcctcctcg
     1921 aggcccggct cggacgcgag cttgaggaga cccgaatccg cgtcgaccgt gaaggcgtag
     1981 tcggggacgc cgttcctccg gacctcgacc gagccctcga tgacgtcctt gtctatcgtt
     2041 atctcggcgg ccgcggccag gcgcctcacg acgatctcct tcgtgaggag gggcgcctcc
     2101 agagcagcga ggatgtcggg gtcgtcctct gagaagtcgg tcgtgtagag atccgccatc
     2161 tcggaaagga atggttgacg gtagtcggcg tgcgtgggga cggtctgccc gcccttcgtc
     2221 acctcgatga agcctcctga gtcgaaacgc gcctcgtaat catcgtccct gaggcccgag
     2281 gcccggtcgc gcacgaagac ctccgcgctc gaggcgccca ggacgggata gcggcaaagg
     2341 acttcggtat tggcgccgaa gacttccggt tcgaagagca ggacgcaacc tgtctgtccg
     2401 cctactgtca aagtccccgc cgagccgtcg tagctcgcgg cgaccctggt cgtagccgcg
     2461 gccgccaggg ctatgaaacc ggtggccgcc gagtaggagt actctcccgc ctccagtcgg
     2521 cgataggcct tgccgtcgct gccttggaag gtaccggcgg agctctccac gtagaggacc
     2581 aggttcgcgg cggcgccgct cgtcgtcgcg aaatacctgc cccgctggaa gccgctcgcc
     2641 gcgacgacgg tctccgtcac ctcggatcgg ccgacgaagc gcttctccac gcggtcggcc
     2701 tggtcgtagc ggagcatggc gctgccggag aaggcctccg tctccacttt ggcggccgcg
     2761 ccgaaggagc ggcggctgcc tgagccgaag gagaggaagg gaagcgcggg gaaggagatg
     2821 ccgtcgttgc cggctcggac ttccttgagc gcctcgccct ctccgccgcg atagccgaac
     2881 gaatacctcg cctccgccag gtcctcgctg acgcgggcct cgacgaacac cttcttgaag
     2941 aggaggaagg agaggaagag gtcgggcctc tgggtgaaca ggaggggctg ggcctcggag
     3001 agggcgaggc ccccgccgcc cgcctccagg ctcgcttcgg cgaggaggct cgcctcccag
     3061 gagccgcgga tgtcgagctc ggcgtcggcc tcgccgcctt cgccgtccag gtccacggcg
     3121 aagaggctct tgggcgcctc cgtgggaagg gtccgcggca cgaaggcgcc ggcgctcagg
     3181 actgtcgccg gatcctcggc ctggcccgaa acctggcccg ctacgcccag gggaaaggcc
     3241 gaggcgagga ccgagccgag gaggagggcc gcggcccggg aagcgcttct ctttaccatc
     3301 gcgaaggcct atgatagtat tatagcacgg gcgggcggta aagccgtccc gaagaagaag
     3361 cgtgatggaa acgagaaacg accccctggc gcggctcctc cgcgtcgcgc tcgacatcct
     3421 catcgtcctg gccgtcgccc tcgtcttcgc cgaggaggcc gccgccggca tgggggcggc
     3481 ctggaccctc cgccgggtcc tcgccctggc gagcgccgcc ttcgacctct tcttcgccgc
     3541 cgagttctcc gcccgcctcg cgctcgccgt ctccgacggc gaggccgggc tctacctcgt
     3601 cgagaagggc ggctggctcg acggagcctc ctccctcccg atcctcgtct tcgtctcggg
     3661 ccccttcctc ttcggcaccc tcgcgggcgt ccatcccgcc gtgtccgtcg tgggctcgat
     3721 gggcgccatc cgcatactcc gcagcctgcg cctcctccgc tcgctccgcc tcctccgctt
     3781 cctccgcgcc tccggcggcg gctccggcct cgcggcgcgc aggcacctgg agaaggcggc
     3841 cggactcgcg ctcggggcgg ccgtcgccgc cgccctcctc atcgaggccg cctccgccct
     3901 cggcgcctgg cccgacgcgc gcagcgtcct agaggacaag cggcgcgcta ccgtggcgac
     3961 cctggccggg gctcccggcg ccgacggcgc cgaggcggcg gcggcggtgg acgcggacct
     4021 cctcctcgtg cgggagaacg gaaagatcgt ctacacccgc tactcctccg aggagtaccg
     4081 gcgccgcttc ggccccgacg agatcggcta tctcgccgac cagggcggga cggaggcttt
     4141 cttctccatc tccgcggaga accgcgccag ggccgcctcg gccctggcct ccgcgttggc
     4201 cgccctcgcg gccttcctgg ccgtcatcct gggctacggc ccctacttcg cggccggcgt
     4261 ctccaagccg gtcaacgcgt cgctcgccct gctcgagggc ggcggggacc gctccggcct
     4321 cttccctcct cccggccggg acgaggagga gcccttccgc ctgtccctgg ccctcgccga
     4381 gtacctggac aggcagcgcg ggatcgattt cgaggcgccg cccctcttcg acgaggccga
     4441 gcttccggcc tccgctcagc aagcctcgtc gccggcccac gagagcgcgc cggccatgga
     4501 gccgccgcgc gccgcgtcga acgccgtgac agccgcggac ccggcgccgc ggccggcctc
     4561 tcttttcgcg gcggccgcgc ggttccgctc cggccagggc gccgcgcccg cgggggagga
     4621 ggagctcgag gccctcctcg atcccgaggc ggggcgcgag ggcccatgat ggtcgtctcc
     4681 atgatccaga tcatcatcga ggtgccggag gccgccacca tcaaggacaa gcgccggatc
     4741 gtcacgatga cgaaggagcg gctcaggacc aagttccgca tatcctgcgc ggaggtcgac
     4801 ctcctcgact ccctcaggtt cgcgcaactc ggcggggcca tagtctcgaa ctcccgggag
     4861 ttcggcgaaa aagtcatgca gaaggcgctt tccttcgtcg aggacgggct cgcgctcaag
     4921 gtccacgacg cccagataca ctcggagctc tacgactgac ggaggttccc ttgctcgatc
     4981 ccatgtccag gcggaggggc gcgctcgccg ccgccgtcct cgcttccgcg gtcctcctcg
     5041 cctcctgcgt cggcgtcgac gcggaggcga ccgtggccag ggacggttcg gggcgcctca
     5101 agctccgcta cgccgtctcg aagatgctcg tctcactggg ggccctggag gcgaacgaga
     5161 ggctcctgcc cttccccgtc tcccgcgagg acttcgagcg cacggtccgg ggcattcccg
     5221 ggctcggcct cgtctcctac gcgcagaagg agaccgagga ggacctcgtc gtcgacgcgg
     5281 aactctcctt cgccacgccc caggccctgg ccgccttcct ggatcccaag ggcgagcgcg
     5341 cggcctactc cgagtcgggc ggggagaagc gcctcaggct cgtcctcgcg gaggggcagg
     5401 ccaagctcga tcccgacctg gacaagctcg tccgggcggc cttcgcgccc tactcggtgt
     5461 cgctctccgt gaagcttccc tccgccgcca agtcggccgg catcggcaag tccgcgaggg
     5521 gcgggctcga cctctcgtat tccagccccg tcgtcgacct ggtgacgagc gggagccccg
     5581 tcgtctggga aatccgctgg tgacatccgc tggcaaagga caaggaggct tgaccaagat
     5641 gacaacgcgc cgcttcgcct tgctccgcgg cctctcgttc gcctcggccc tggccgcggc
     5701 cctcctcttc gtcgcctgcg ccaccgcgcc cgccttggcc ggcccgcggc cggccccggg
     5761 cggcttcgcg cccgcggggt ccgccgccga tgcccccgcg aaggccgcgc caggcgctcc
     5821 cgccgtcatg gcagccggac cctccgccgc ggagacggaa gcctcctccc tcatggacaa
     5881 ggcgcgggcc gccatcgact cgaaccacct cgccgagggc atcaggtcct acgtgtccgt
     5941 catcgcgatc cacgaggagt ccccctcccc cgccacgaag gccctcgccg acgcggccgc
     6001 ctccgagctc gcccgcatcg gcgcgcggct gtccatagag cccggcagcg agtgggtcga
     6061 cgcgaagggc ggccagctcg cgggcgcctc gcgggccgtg ggcaaggaag gcgccctctc
     6121 gccctccgtc tacctctacg agaatttcgg cgcgggcaag tcgcccgtcg ccgacgcgcc
     6181 catctacttc gagttcgcgc ggaactcggg ctccgtggtc tccctggtca cgaccgacgc
     6241 ctacggcaag gccaacacca cgatcgcccg cctcgacgag ccgggcaagg aggccctggt
     6301 ccgcgcctat cccctcttcc gggcccgcgg caggacctac gccttcaaga gcgtcttccg
     6361 cgacttctcc ttccttccgc cggccaacgt cgcgaggatc gtcgccctgg agacgggcga
     6421 attcggcgcg accgacaatc cccaggtcgt cgacgccctc gcctccgtcc tcaagcccct
     6481 cggcctccag ttcgtgccct acaacggcaa gctggcccag gactccttca ggcgcgcctt
     6541 cggcggggac agggccgccc tctcctcctt cggcgccgtc gccgaggagc cctacgcggc
     6601 cttcgtcctc gtcgaggtct ccccaacgcg gcagatggag ctcaacggca agaagtacaa
     6661 catcttcacg gccgaggccg cagcgacctt caggctcgtc cgctccgacg gcacgatcgt
     6721 cttcgccctg cccctcgacc ggctcaaggg ccaggggggc acgcccgagg ccgccgtcca
     6781 ggacgcgctg cgccgcgccc gcgaggcgct ctcgcccgag ctcgagaagc ggctgcccgc
     6841 gatcaaggcg gctctcgaga aggagtaggg tccccgcccc tgtcgacgag ccgcgcgcgg
     6901 cggaggctcc cgagccccct ccgctcgatc tcgaagacgg cctcctcggc cgccctcttg
     6961 cgcgccgagc cgccgtcgaa gagctcgccc tgcaggggcg tcccgcgcgg ctcgagctcg
     7021 gcgaagccga ggccgaggag gcgcaccgcc ctgcgcccgt cccaggccga gtcgaggagg
     7081 gccagggccg cggcccgcgc ctcctcggcg ctcgcgaagg gctcgggcct cgacctcctg
     7141 cgcgtcaggg tgctgaagtc ctggaacctg agcttgatga agacgcgcct ggaccgcagg
     7201 tcctcgctcc agagccggta ggcgagctgg tcggcgagct ccacgaggag ggtctctagg
     7261 aggctccggt ccgagacgtc gagctcgaag gtgcgctcgg cgctgaccga gcggctctcc
     7321 gcctcgcgcg gggcgatgcc cggatcccgc cccctgaccg cctcgtagag aaatcgcccg
     7381 cccgagcccc cgaagagcga agcgagggat cgttcgccca tcgcggcgag ctgggccgtg
     7441 gtccggatgc cgagctccgc gaagcgctcc tgcgtcttct cccccgcgcc ccagagcttg
     7501 cccaggggca aggcgagtat gaaggccgcc tccccgcccg gctcgacgac gaccaggccg
     7561 tccggcttcc tgaggcccga ggcgatcttc gcgacatagt ggttggcggc caccccgacg
     7621 gagatcgaga gccctgtgcg ctcgcgcact cgcgccttga gggcggcggc cgcggcttcc
     7681 ggcgggcccc agagccgctc ggtgccggac atgccgagga aggcctcgtc tatggagact
     7741 cgcctgacct cgggcgagaa ttcgccgaag gcctccatca ccctctccga gatctccgcg
     7801 tagcgctcca tcctgaccgg gaggaaggcc gcgcccgggc agcggcgcca ggcctcggag
     7861 atcggcatgg cggagtggat cccgtaggcg cgggcctcgt aggagcaggt ggagacgacg
     7921 ccgcgcctcc ccggcgcggc tccgacgacg acggggcgcc cgcggagctc cggccggtcg
     7981 agctgctcga cggaggcgaa gaaggcgtcc agatcgacgt ggaagacgac gtcgcccatc
     8041 gcgcgcgcct agtccccgag ctcggcggag gccctgagcc cgaagcgccc ggggacgacg
     8101 tccgctcccc gcgcctcccc gtagggcacg aggggctcga ggtaggaggc ttcggccacg
     8161 aaggccgcgg agaagcccct gggggccgcc agttccacga ccagggggtc gggagggttg
     8221 tctccgacga agatctcggc ccggcgcccg tcgatggaga cgcggtaggg aagcgagcag
     8281 tcgtagatcc gcaggtcctc cccgccctcg aggcgcaggc ggagcgagtt gggcggcttc
     8341 gcgaagtcga gcgtggtcct gaagaggctc ctccccagga aggaccggcg ctccgcgcgg
     8401 atcgagaccc aggccgagcc ctcagcgcct acgaaggccg ccgagtcgcc gccggaggag
     8461 agttccaggg cctcgccgcc gcggtcgagc tccaccctgc cgatccttct ccgcccctcg
     8521 agcctcgcct cgaagctccc cttgtcctgg tcgacggact ccgagagcgt cagggcggcg
     8581 aggttcctgg acggggacag gaccgcgtag cccagggccg agccctcgac ggccagggcg
     8641 aggagggcga agaggagggc ctccgccttc ttgcccttgg ccgtatgggg cctgaagaag
     8701 aggaggggac tcgcggtgaa ggcgttgaag ggcagcgccg ccgcggccag caggagggct
     8761 cccgggaggc tcggcgagac gagcctttcg tagagcccgt actcgggcgc cacggccagg
     8821 tccgcgacga ggaggaggaa gggcgcgtac atgagggcgt aggccgcgag gctcgtccag
     8881 ggccgcctgg ccgcgagcga ggcgcccacg accaggaagg cccaggcgaa gtagaaggcc
     8941 aggggcgggc tcatcgcgga gaaggcgaag acgtcgaggc ctaggcagac gagggaggcg
     9001 aattcgtaga agtaggggtt ggaggtgagg aggcgcctgt ccacgaggag ggagatggcc
     9061 gcgaggcaga ggagcaggga gcccaggagc ctcgcgccgg cgaaggccct cggagccagg
     9121 cgccagccct ccttcgagcc gaggaccagg gactcgaggc cggccgcgag gcggcacagg
     9181 gcgtagacgg ccgtcgtggc cgcgaacagg accaggaact gcccgagcat cgaggggacg
     9241 cggcgggcca gggccttggc ggcgtctcgc ctcgcgaggg tgaaggccgc gaggagggcc
     9301 gcgacgcccg cgcagaacgc gagaaggagg gagacgtagg ccttctccct gaggacgaag
     9361 gaggcgcgcc cgagctggaa gctcaggtag tgcctgtccc aggtctcgag gaagccttcc
     9421 gcgttgcccg cgaccaggcc gtcgacgagg cgcgagaagg cggcccgagc cgcgcccggc
     9481 gaggcgggcg gcccttcctc cccgcggagc tcgacggccg gaatgccggc caggaggtag
     9541 ggggaaatgt ggctcggggc gtccgagagg ccgagcctgg agacctgcat gcggttcgcg
     9601 aagaggcggt aggggaagcc ggaggcggcc gccgccgccc tggtccgctc gtagtgccaa
     9661 tagggcgaga ggaggccgcc gcctgagttg aggaggccga tggccgaggg catggcgtcg
     9721 aagtcgaggt agaccacggc gaggggcggg tcgccctcct tcccggcgat ccaggactcc
     9781 gagccgtagc ccagggagcc cggctcggag gccctgccgc gccgctccgc ccccaggaag
     9841 aggaaggaga cgccgattgg aagctcgccc ctcccgggcg ccgcgagcct ctcgatctcg
     9901 gccagggcca gggccagccc cgcgtcgccc tgccccggct cgccgtcctc gaaggccccg
     9961 agaggcaggg cgaagacgag gcggtccggc ctcgtcccct cgaagccgac ctcgaagagg
    10021 ctcgagtagg aatgcccggc gccgggcctc gcgaggtcgt agtccctgag cctcgcgccc
    10081 gccgcggcgc aggctgcccg caggtaggcc gcagccttcc gctcggcctc ggagttctcc
    10141 cggcgaggcc cgaaggaggc cagggcggcg agatggagcg ccgcgcggtc cgagccgagc
    10201 gaggaggccg cggctggcga cgcgccggga gccgcgccgg gagccgcgcc ggagcctgac
    10261 gcgggacctg gagccgcgcc ggagcctgaa gctccgggaa ccgccgtggc ggcggacgcg
    10321 ggcccgggcg ttgcgaggac ggcggacgcg ggcccgggcg tcggcacgga gaggggagac
    10381 tcggctcccg cgggcgcggc cccgcccagg aggaagacag cgagggccag gccggcgaga
    10441 ggggggatct tcacgggctt gagcataggg agggctctct cctttcgtca atactgttta
    10501 ccccgtgccg ataatagtct atactggcgc ggaatcggca ttcgagccgg ccgaggcgaa
    10561 gggcgtgcat gcaggaacgt aagagcgtat tggatctgcc catcaaggtg gtcctcaccg
    10621 aggaggggac ctctttcttc atccgcaagg ggaagagcct caagaagttc aagctcgccg
    10681 acaaggtcga ggagtacggc atcgtcctcg acagcttcag ccccgcctcc ctccagcgca
    10741 tgatgctcgt cgactacatc tccaaggtgg agatctccaa gggggagttc atgtcctccc
    10801 gccaggagat catggacctc tccaagctcg tggtcttctc cgtcctctac cgccagtacg
    10861 actcctacat cttcgcgcgg gtcctcgcga gcgacgtcat caagaagtgg aaccgcctca
    10921 acccggccaa catcatcgac gagaagacga ggatcaacga cggcttcctc cagacggtcc
    10981 tcaaggagaa ggacaaggag atccgcgaga tcaaggagca gatcctcgcc ccgatgtgcg
    11041 cctacatcag caagaactcc tccctcctcc ccgaggagaa gaacatccag ctcctcctct
    11101 ccgagaaatt cctcaacaac ctccggccct tcacctggtt catcatcgcc aagttcaagg
    11161 gggcggacgg ctacgaccag ctcctcaagg acatccgcac ctcgctcgcg gagtacatgg
    11221 agaagtccaa gatcgcggag tacgtctccc tcatgatcat ggagctcgcg atgaacgcgg
    11281 agaactccaa cctgaagcgc gaggccaagg ccatcttcaa gggcgccgtg gacatgaacg
    11341 ccgtgctctt cgaccccaac atacgcaggc aggtcatcga ctccctggag cggagggggg
    11401 agctcgtcta cctggcctgg aagatcggct cccggaacgc ctccatcggc acccagggcc
    11461 gcctccagat cacgctcttc aacaaggagt ccgagtacca gaacatgaag gaggccttcg
    11521 acgagaagaa gaacgccgac ctcaagaaga agagcctcca ggacttctac aagcagctcc
    11581 ccggcggcga ggccaacgcc gagctcggcc tctactacct ctcctacctc tccgaggcct
    11641 gcgagaaggt gaacgtcaag ttcgagtccc tcgtcaacca gctccccggc tccgacctca
    11701 cggtcatgac cctcgtcctg aacctctagc cctcctgccg cgttaggcgc ttcccaaaag
    11761 ccatccatgc cccttccggc cgcgccagga gggcctttcc gcggaaaggg cccgcgcgcg
    11821 ggcctggcct cccctcccgg gggcgcgccg ggcgttcctt gacatagaat gccctttgct
    11881 gtagcttgac acggtattct acgtggactc tacccttagt acggaggaat aagcagagat
    11941 ggccaagacc aagtatgtct acgcattcgg tggcggcgtc gcggagggcg ccgggatctc
    12001 caaggatatc ctcggcggca aaggcgccgg actcatggag atgaccaaga tcggcctgcc
    12061 cgtccccgcc ggattcacga tcggcatccc cgcttgcgag gagtactaca aggccggccg
    12121 caagctcccc aagggcatcg tcgacgaggt caagctcgcc gtggccaagc tcgagaagac
    12181 cgccggcaag aagctcggcg actccaagga ccccctcctc gtctccgtcc gctccggcgc
    12241 cgcccgctcg atgcccggca tgctcgagac gatcctcaac ttgggcctca acgacaagtc
    12301 cgtcgagggc ctggccgcca ggaccgccaa cccccgcttc gcctacgaca gctaccgccg
    12361 cttcatccag atgtacgcga ccaccgccat gggcctctcc aaggagccca tggaggagat
    12421 gctcgccgac atgaagaaga agctcggcgt caagaacgac accgacctct ccgccgacaa
    12481 cctcaagaag ctctgcgagg agttcaagga cttctacaag cagaagaagg gccaggcctt
    12541 cccccaggac ccgatgctcc agctctgggg cgccatcgac gc
//
LOCUS       DAOOSJ010000047        12576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3354_length_12576_cov_5.536379, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000047 DAOOSJ010000000
VERSION     DAOOSJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_12576_cov_5.536379"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /gene="murD"
                     /locus_tag="PLG14_03845"
     CDS             complement(<1..1344)
                     /gene="murD"
                     /locus_tag="PLG14_03845"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOX48295.1"
                     /translation="MGLGLNGGGLASARFLAERGALVTVTDMKDEAALAAPLGALAGL
                     PIRFVLGRHDLEDFRNADLVLKNPAVRPDSPYLLASRAVETDLSLFLRLSRAKIIAVT
                     GSKGKSSVSSALYHGLKENGRRAFLGGNITVSPLTFIDEAGEDATVVLELSSWQLADM
                     KGLGVLKPEVAVLTSMMPDHMNRYSSMEEYMADKRLIYADQDGTCHTICNRDDAWNRS
                     FAAETRGRVSWYADEREEGLPGAWLEGPGGRGLYSADGSPSGAETILPEKLLVPGRHQ
                     RKNLLGAALALRVFGLPAEGVAKAMASFPGVEHRLELFAEAGGVSWYNDSAATIPQAV
                     EAALGSLDAPVVLITGGTDKNLDFEPVRAAFRKPKAVILLSGSGTEKLRPLLEADGVA
                     YEGPYDGLEKAVAAAAARAGRGDAVLLSPGCTSFGMFLHEFDRGRKFKETVRALLE"
     gene            complement(1379..2338)
                     /locus_tag="PLG14_03850"
     CDS             complement(1379..2338)
                     /locus_tag="PLG14_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48296.1"
                     /translation="MEQLLNEIRVSYMDPEINRLLGSIPEAAPDGRAKTHYNSGEELF
                     LELETELFVPRLPIHHDVRVPEPSASYAASLRDVARQLAELLPGAFRGLTYYFDPAEI
                     LKPCFYRLYKVEESVYLYLLRLDLVHRAFEGEVLEPGTNDVTPAYRTKRLYAESEIIP
                     LDAVMWELGRVKAFRIRQLVSNTWIGETGRGYLVRGIWMDAGLSKFFSRLFLPDGART
                     YPFYPFFCKYKTVCAGAPSLTTSYRRKVLPLLHRAIGFLVPEMERIQNSLKNGDFSET
                     MPEFAELKARVPAAWREIFSGFASKPYLNERDLKEFSLEIPDL"
     gene            2446..3507
                     /locus_tag="PLG14_03855"
     CDS             2446..3507
                     /locus_tag="PLG14_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOX48297.1"
                     /translation="MKATTYALAAGLLAALTPSWAQSPAKAAAEIVYFEGKDLSVLRA
                     GKPLSVGDPIGLELFQDDQVQTGPKSTAEILLKAKGAVVKLSENTVLSLRGLGAETTS
                     LELLYGRVRSKVAKLAAKDSFEIRSSSVVAGVRGTDFGCDLVAPRSLSGAAGSGSAPL
                     VRVYCFSGSVEVSLQPLATPAAAAGPAAAASAGAPPAPLPLPAPILVEAGKMVLVDLG
                     SSPAASAPPPLEAKPLDEDLRRFWTANDFESLEVSGAAVPALLGAAVPAPAAFDFAAA
                     RASLARKNASLTGGLALAAVGAALGASSLFIAPNDAELGSALGLAGALCSGASVPVLI
                     FSLSVNPLAAPRKSGEGER"
     gene            3504..4316
                     /locus_tag="PLG14_03860"
     CDS             3504..4316
                     /locus_tag="PLG14_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOX48298.1"
                     /translation="MSAWFLGLPHPAQALLATLFTWGLTALGAAMVFFFKSINRKVMD
                     GMLGFAGGVMIAASFWSLLAPSIELAESMGLPGWLPAVVGFLAGGLFLKLVDKVLPHL
                     HIEAKLEEAEGPKTTWKRSVLLVLAITLHNIPEGLAVGVGFGALGAGIPSATMAGALA
                     LAMGIGLQNFPEGAAVSIPLRREGMSRWKSFWYGQLSGVVEPVAGFLGALLVVAMRPL
                     LPYALAFAAGAMIYVVVEEVIPESQGSGNSDISTLGALGGFALMMFLDVALG"
     gene            complement(4458..6242)
                     /locus_tag="PLG14_03865"
     CDS             complement(4458..6242)
                     /locus_tag="PLG14_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48299.1"
                     /translation="MKDMKKVSHISWIWGVWRERKGLIALLFALTLLSSAVAVAYPYL
                     SKLLIDMLEGLLSKPGGPEEAGRAIDRLAGLVLAVGASGFVASLFPGVRGAVNSVFEH
                     LIRMKFFANVMGKDRRFFQAFRSGDVTTRLTDDLYDFPKLSWFLCSGIFRAVESISKI
                     AFCLAAMLLLNWRLTLLSLIPLPVMIAVFYVAQDRIYDTFRKNQEAISDINSQLEMSF
                     SGVRIIKAYSCERKYERFFSEALAKRFGTEMRVTRLGTLIDLIYMYIDYAAQIGIIFA
                     GGYMAVKGDISIGTFYAFYNYLGMLIYPILDIPQLFVSGKRAFVNIDRLEEMRAFPDP
                     VRPASPFPVARVERIEARGLSFSYEGREEPALDGVDFSLERGERLAVIGPVGSGKSSL
                     VKMMAGLLEPSSGEILLNGRPVREADRESLAAALGYVPQEALLFSGTIRDNVAFGAPG
                     EGRSIEDEAFRAAVAEAQLAGEIASFSDGEATLLGQRGVALSGGQRQRLAIARALARK
                     PSLLLFDDITASLDAANEERLWRALDSRSRDLSCVVVSHRLSTLQYADKVLFLDRGRV
                     SAFGRHEELLRVNAAYRSFIEEQMTMKA"
     gene            complement(6239..8197)
                     /locus_tag="PLG14_03870"
     CDS             complement(6239..8197)
                     /locus_tag="PLG14_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48300.1"
                     /translation="MPKSTRSRRLAGYVGDFKGLFLLGFLALSLVTAGQLCGPLILRS
                     IIDESIPKRDLAGMLLRALAYLGLVVGMGGLTYYGTMVIARLGLEVVTRIKKDLFSHF
                     LTLPVAYFDKHPVGELMARVESDTEKVKELFSRIGITLATNVLFFGGMLAVCFSMEPR
                     ITAYIAAAIPVALAFVIFFFDKLRVLYEASRKRYAAVTARVAEFVQGSEVLRAFGREA
                     WAVSALDEESKGKRDIEVKASMIEYSAMGALGFLIGPCFMAGIVKLLAPEILAGAMTL
                     GTLLVFLEYGRRLFDPLMAIAENVRGIQQARVSLKRIFDILSLPPEEGRAGAARDVDA
                     RNGAPLAGDARAVAAFEHEIEFRKVWFRYKEEEWVLKDVSFTIPKGKCLALVGPSGSG
                     KTTAVSLLCRFYEPTRGEILVDGRPLRELDLEAWRRKIGLVLQDVYLFPGPVLENVRV
                     YDDSVTETAVRGALDAVQASDFVERLPDGLSSEIRERGSNISSGEKQLLSFARALAFG
                     PEIVVLDEATASIDVKTERRIAEGMKGLLSGRTSVIVAHRLSSIAGADEILFFKDGRI
                     AARGRHGELLSAFPEYAELVRLQFPECGPGGAGGGRGPEGADAACPDAEGAGAGGAAA
                     PGAEPAGARSEGSPTEGALAAQAPGVEA"
     gene            8345..10498
                     /locus_tag="PLG14_03875"
     CDS             8345..10498
                     /locus_tag="PLG14_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48301.1"
                     /translation="MRRIERHPIVSPAAGRREVSFSFDGESLTGYEGESLSSALAAAG
                     VVAFSRHRKGEAPQGIFCANGQCSQCTVVIDGLAKKACVTPLAAGMDVRSLSGLPVLP
                     EDDTPFAGAEKRSLSADVLVIGGGPSGLSAAAELARLGLSVILADDKASLGGKLVLQT
                     HKFFGSEEDCYAGTRGVDIAKRLEEELRSLPNVTVLANSPVVGIYKDRKAGVYVDYQR
                     YALVDFSALVVATGAREKSVLFPGNDLPGVYGAGAFQTLVNRDLVRPARRVLIVGSGN
                     VGLIAAYHALQAGIEVAGIVDVLPKAGGYKVHADKIVRMGVPIYLGTTVVAVEGSGRV
                     ERATTARVDSSYRPLLETARTYEVDTVLVAAGLSPCDEYYRMARAFGFLAVTAGDAEE
                     IAEASSAMFGGKIAALSLAKLLEKKVEIDPSWVAKREVLKSRPGDRLPREAVAAGDSW
                     KPVFFCEEEIPCNPCTTVCPTKSIRLRPRKGSLLDLPYFEGKDCRGCAACVAACPGLA
                     VSLVRRIDEAWTEVLLPYEFLADFEAGARLPLLDMEGAFLEEAELLKKTFYKKHKTWV
                     LSLKVSAANAPRAIGVRVQAESATRPLPEPSFSHLPDEAVVCRCERITVGEIVDFVKR
                     NDVRDVNQLKSLRVGMGACGSKTCSVLLPQVLRKAGLDPAAVAPGSLRPLGMEVPMGA
                     LVNEGGYDGGGAGSEGADRKPRSAAAAAAGARGEP"
     gene            10495..11667
                     /locus_tag="PLG14_03880"
     CDS             10495..11667
                     /locus_tag="PLG14_03880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48302.1"
                     /translation="MRSYDIVIAGAGSIGVPLSYYCAKKGFKVAVVEKEASWGRGQNR
                     AAIGGLRATHSDPAKIRICRESIDIVSGMKEELGIDVEWRRGGYLFVAYDEEREAAFR
                     KLLEIQKAAGLDIDWVGPGRVAELSPGINMKDLRGGTFSPGDGYASTLMTATAFHRLA
                     MEAGVDFFFGETIQGLEVSGGKVKSLKTGKESFSAPLFVNAAGADAADVAAMAGAELP
                     IHPDCHEAGVTEPVERFLEPMVVDIRSDAESGNYYFYQAATGQVVFCITPRPQVWGKD
                     KDSTSAFLPLCVRRMLELYPRLRNLKVRRTWRGMYPMTPDGFPIVGYPREADNFLQAV
                     GMCGQGFMMGPGLGRILASAIASGSSPARPEGQTEYGFVFEELAPYRKFEGMELLK"
     gene            11864..12400
                     /locus_tag="PLG14_03885"
     CDS             11864..12400
                     /locus_tag="PLG14_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOX48303.1"
                     /translation="MPLSTFVLGELSRYGPLHGLELKRRFETAGIVSWGPVAATSVYR
                     ALAGLERKGLVRRIQGRFRANRKPYAVTLAGRREFAFRLRAELKSPRTYRNPLNIAVE
                     YARLLPPRELAGVMEERLGALRGLERGVSERLGGFSPDAAPAPGEVLATCERILWKTR
                     AEIRFAVRMLSLARASPG"
BASE COUNT         1878 a         4367 c         4421 g         1910 t
ORIGIN      
        1 ttcgaggagg gccctgaccg tctccttgaa cttcctgccg cggtcgaact cgtggaggaa
       61 catcccgaag gaggtgcagc ccggggagag gaggacggcg tcgccgcgcc ccgcgcgggc
      121 ggccgccgcg gccacggcct tctccaggcc gtcgtagggg ccctcgtagg ccacgccgtc
      181 ggcctccagg aggggacgga gcttctccgt gcccgagccc gagaggagga tgacggcctt
      241 cggcttcctg aaggcggcgc ggaccggctc gaagtcgagg ttcttgtcgg tgccgcccgt
      301 gatgaggacg acgggcgcgt ccaggctgcc tagggcggcc tcgacggcct ggggtatggt
      361 ggcggcggag tcgttgtacc agctcacccc gcccgcttcg gcgaagagct ccaggcgatg
      421 ctcgacgccc gggaagctgg ccatcgcctt cgccacgccc tcggccggca ggccgaagac
      481 gcggagcgcc agggccgcgc ccaggaggtt cttccgctgg tgccgtcccg ggacgaggag
      541 cttctccggc aggatggtct cggccccgga aggggagccg tcggcggagt agagcccccg
      601 ccctcccggc ccctcgagcc aggcgccggg caggccctcc tcgcgctcgt ccgcgtacca
      661 gctcaccctg cctcgggtct ccgcggcgaa ggagcggttc caggcgtcgt cccggttgca
      721 gatcgtgtgg caggtcccgt cctggtcggc gtagatgagc cgcttgtccg ccatgtactc
      781 ctccatggag gagtagcggt tcatgtggtc gggcatcatg ctcgtgagga cggccacctc
      841 gggcttgagg actccgaggc ccttcatgtc ggcgagctgc caggaggaga gctccaggac
      901 gaccgtcgcg tcctcgccgg cctcgtcgat gaacgtgagg ggggagaccg tgatgttgcc
      961 gcccaggaag gcgcggcggc cgttctcctt gagtccgtgg tagagggcgc tcgagacgct
     1021 cgacttgccc ttggagcccg tgacggcgat gatcttcgcg cgcgagaggc ggaggaagag
     1081 ggagaggtcg gtctccaccg cgcgggaggc gaggaggtag ggcgagtccg gcctgacggc
     1141 ggggttcttg agcacgaggt cggcgttccg gaaatcctcc aggtcgtggc ggcccaggac
     1201 gaagcggatg ggcaggcccg ccagggcgcc cagcggagcc gcgagggcgg cttcgtcctt
     1261 catgtccgtg accgtgacca gggctccccg ctcggcgagg aaacgggcgc tcgcgaggcc
     1321 gccgccgttc aggcccaggc ccatcaccgt gaccttgagg ccgcgcaggt cctgggcgct
     1381 agagatcggg gatttcaagg gagaactcct tcaggtcgcg ctcgttgagg tagggtttcg
     1441 aggcgaagcc cgagaagatc tccctccagg cggccggcac cctggccttg agctcggcga
     1501 actcgggcat tgtctccgaa aagtcgccgt ttttcaagga gttctggatc cgctccatct
     1561 cggggaccag gaagccgatg gcccggtgga ggagggggag gaccttgcgg cggtaggagg
     1621 tcgtgagcga aggcgctccc gcgcagacgg tcttgtactt gcagaagaag gggtagaagg
     1681 ggtaggtgcg ggcgccgtcg gggaggaaga ggcgggaaaa gaacttggag aggcccgcgt
     1741 ccatccagat gccccgcacg aggtagccgc ggccggtctc gccgatccag gtgttcgaga
     1801 cgagctgcct gatcctgaag gccttcaccc gcccgagctc ccacatgacg gcgtccaggg
     1861 ggatgatctc gctctccgcg tagagccgtt tcgtgcggta ggcgggcgtc acgtcgttcg
     1921 tgcccggctc caggacctcg ccctcgaagg cgcggtggac gaggtcgagc ctgaggaggt
     1981 agaggtagac ggactcctca accttgtaga ggcggtagaa gcagggcttc aggatctccg
     2041 cggggtcgaa gtagtaggtg aggcccctga aggcccccgg aagcagctcg gcgagctggc
     2101 gggcgacatc cctgagcgag gcggcgtagg aggcgctcgg ctccgggacg cggacgtcgt
     2161 ggtggatggg cagccggggc acgaagagct cggtctcgag ctcgaggaag agctcctccc
     2221 ccgagttgta gtgggtcttg gccctgccgt cgggggcggc ctcggggatc gagccgagga
     2281 ggcggttgat ctcggggtcc atgtaggaga cccggatctc gtttaaaagc tgctccatgg
     2341 taatcccgta cactatagcc cttcccggac caggatgcaa aagaaaaggg cggcggaccc
     2401 cgcgccgcct ttgtaatccc cggaggcccg gcttatattc gaagcatgaa agccacaacc
     2461 tacgccctgg ccgcaggcct tctcgcggcc ctcacccctt cctgggccca gtcgcccgcc
     2521 aaggccgcgg ccgagatcgt gtatttcgag ggcaaggacc tctccgtgct ccgcgcgggg
     2581 aagcccctct ccgtcggcga ccccatcggc ctcgagctct tccaggacga ccaggtccag
     2641 acgggaccca agtcgacggc tgagatcctc ctcaaggcca agggcgccgt cgtgaagctc
     2701 tccgagaaca cggtcctctc cctgcgcggc ctgggcgcgg agaccacctc cctcgagctc
     2761 ctctacgggc gggtccgctc caaggtcgcg aagctcgccg cgaaggacag cttcgagatc
     2821 aggagctcct ccgtcgtcgc cggcgtccgg ggcacggact tcggctgcga cctcgtcgcc
     2881 ccgcgcagcc tctcgggcgc ggcggggagc gggagcgccc ccctcgtgcg ggtctactgc
     2941 ttctcaggca gcgtcgaggt ctccctccag cccctcgcca cgcctgccgc cgcggccggt
     3001 ccggcggcgg cggcctcggc gggcgcgcct cccgcgcccc tccccctgcc ggcgcccatc
     3061 ctcgtggagg ccggcaagat ggtcctcgtc gacctgggct cctccccggc cgcctcggcg
     3121 ccgcctcccc tcgaggccaa gcccctggac gaggacctga ggcgcttctg gacggccaac
     3181 gacttcgaga gcctcgaggt ctcgggcgcg gccgtccccg cccttctcgg cgccgccgtg
     3241 cccgccccgg cggccttcga cttcgccgcg gcccgtgcct ccctggcccg gaagaacgcc
     3301 tcgctgaccg gaggcctggc cctggccgcc gtcggcgcgg ccctcggcgc ctcctccctc
     3361 ttcatcgcgc ccaacgacgc cgagctcggc tcggccctgg gcctggccgg ggccctctgc
     3421 tcgggggcga gcgtccccgt cctcatcttc tccctctcgg ttaatcccct ggccgcgccc
     3481 aggaagagcg gggaggggga gcgatgagcg cctggttcct cggcctgccc catcccgccc
     3541 aggccctcct cgccaccctc ttcacctggg gcctgactgc cctgggcgcg gcgatggtct
     3601 tcttcttcaa gtcgatcaac cgcaaggtca tggacgggat gctcggcttc gcgggcggcg
     3661 tcatgatcgc cgcgagcttc tggtccctcc tcgcgccgag catcgagctc gcggagagca
     3721 tgggcctgcc cggctggctg cccgccgtcg tcggcttcct cgcgggcggc ctcttcctga
     3781 agctcgtgga caaggtcctc ccccacctcc acatcgaggc gaagctcgag gaggcggaag
     3841 ggcccaagac gacctggaag aggagcgtcc tcctcgtcct cgccatcacc ctgcacaaca
     3901 tccccgaggg cctcgccgtc ggcgtcggct tcggcgccct tggcgcgggc atcccctcgg
     3961 ccacgatggc gggggccctt gccctggcca tgggcatcgg cctccagaac ttcccggaag
     4021 gcgcggccgt ctccataccc ctgcgccgcg agggcatgtc gcgctggaag agcttctggt
     4081 acggccagct ctcgggcgtc gtggagcccg tcgccggctt cctgggcgct ctcctcgtcg
     4141 tcgcgatgcg ccccctcctg ccctacgccc tcgccttcgc ggcgggcgcc atgatctacg
     4201 tcgtcgtgga ggaggtcatc cccgagtcgc agggctcggg caactccgac atctcgaccc
     4261 tgggcgccct gggcggcttc gccctcatga tgttcctgga cgtggccctg ggctgaaggc
     4321 cgggccgctg tcggccctta gccaggccgg ccgcgcccgg agcgcgggcg gccgtccggc
     4381 tccgcgcgtc gaaagaaaga aggggcgccc cgcgggcgcc ccttctttct tgcctgcgtc
     4441 cgcggacgta ggccggctta ggccttcatc gtcatctgct cctcgatgaa ggaccggtag
     4501 gcggcgttga cgcgcaggag ctcctcgtgg cggccgaagg cggagacgcg gccgcggtcc
     4561 aggaagagga ccttgtccgc gtactggagg gtggagaggc ggtgggagac caccacgcag
     4621 gacaggtcgc ggctccgcga gtccagggcg cgccagagcc tctcctcgtt ggcggcgtcc
     4681 aggctcgccg tgatgtcgtc gaagagcagc aggctgggct tccgcgccag ggcgcgggcg
     4741 atggccaggc gctggcgctg gccgcccgag agggcgacgc cgcgctgccc gaggagggtc
     4801 gcctcgccgt cggagaagga ggctatctcg cccgcgagct gggcctcggc caccgcggcg
     4861 cggaaggcct cgtcctcgat gcttcggccc tcgcccgggg cgccgaaggc gacgttgtcg
     4921 cgtatcgtgc cggagaagag gagggcctcc tgggggacgt agcccagggc cgcggcgagc
     4981 gactccctgt ctgcctcccg gacgggcctg ccgttgagga ggatctcgcc ggaggagggc
     5041 tccaggaggc cggccatcat cttcacgagg ctcgacttgc ccgagccgac ggggccgatg
     5101 acggccaggc gctcgccgcg ctccagcgag aagtcgacgc cgtccagggc gggctcctcg
     5161 cggccctcgt aggagaagga gaggcccctc gcctcgatcc gctccacccg cgcgacgggg
     5221 aagggggagg cgggccggac ggggtcgggg aaggcgcgca tctcctccag cctgtcgatg
     5281 ttgacgaagg cgcgcttccc ggagacgaag agctggggga tgtccaggat ggggtagatg
     5341 agcatcccca ggtagttgta gaaggcgtag aaggtgccga tggagatgtc tcccttcacc
     5401 gccatgtagc cgcccgcgaa gatgatgccg atctgggcgg cgtagtcgat gtacatgtag
     5461 atgaggtcga tcagggtgcc gagcctcgtc acgcgcatct cggtgccgaa gcgcttggcc
     5521 agggcctcgg agaagaagcg ctcgtacttc cgctcgcagg agtaggcctt gatgatcctc
     5581 acgcccgaga agctcatctc cagctggctg ttgatgtcgg agatggcctc ctggtttttc
     5641 cggaaggtgt cgtagatccg gtcctgggcg acgtagaaga ccgcgatcat cacggggagc
     5701 gggatgaggg agaggagggt gagcctccag ttgaggagga gcatcgccgc gaggcagaag
     5761 gcgatcttgc tgatcgactc gacggcgcgg aagatgccgg agcagaggaa ccaggagagc
     5821 ttggggaagt cgtagaggtc gtccgtgagc cgcgtcgtca cgtcgccgga gcggaaggcc
     5881 tggaagaagc ggcggtcctt ccccatcacg ttcgcgaaga acttcatccg gatgaggtgc
     5941 tcgaagaccg agttgacggc gccgcggact ccggggaaga gggaggccac gaagcccgag
     6001 gcgccgacgg ccagcaccag gcccgccagg cggtcgatgg ccctcccggc ctcctccggg
     6061 ccgccgggct tcgacaagag gccctccagc atgtcgatga ggagcttcga gaggtaggga
     6121 taggcgacgg cgacggcgct cgagaggagg gtgagggcga agaggagggc gatgaggccc
     6181 ttccgctcgc gccagactcc ccagatccac gagatgtgcg aaaccttctt catgtccttc
     6241 atgcctcgac tcccggggcc tgcgcggcca gggcgccctc tgtgggcgag ccctcggagc
     6301 gcgcgcccgc gggctccgcg cccggagccg cggctccgcc cgcgcccgcg ccttcggcgt
     6361 cagggcaggc cgcgtcggcg ccttccggcc cgcgcccccc gcccgccccg ccggggccgc
     6421 attcggggaa ctggagccgg acgagctccg cgtattcggg gaaggccgag aggagctccc
     6481 cgtggcggcc cctcgcggct atccgcccgt ccttgaagaa gaggatctcg tccgccccgg
     6541 cgatgctgga gagccggtgc gcgacgatga cggaggtgcg gcccgagagc aggcccttca
     6601 tgccctcggc gatgcgccgc tcggtcttca cgtcgatgct ggccgtcgcc tcgtccagga
     6661 cgacgatctc cggcccgaag gccagggcgc gcgcgaagga gaggagctgc ttttccccgg
     6721 aggagatgtt cgagccgcgc tcgcggatct cggaggagag gccgtcagga aggcgctcca
     6781 cgaagtccga ggcttgcacg gcgtccaggg cgcctcgcac cgccgtctcc gtgaccgagt
     6841 cgtcgtagac gcggacgttc tccaggacgg gcccggggaa gaggtagacg tcctggagga
     6901 cgaggccgat cttgcgcctc caggcctcca ggtcgagctc cctcaagggg cggccgtcga
     6961 ccaggatctc gccgcgcgtg ggctcgtaga agcggcagag gagtgagacc gccgtcgtct
     7021 tgccggagcc cgaggggccg accagggcca ggcacttgcc cttgggtatc gtgaaggaga
     7081 cgtccttgag gacccactcc tcctccttgt acctgaacca gaccttcctg aactcgatct
     7141 cgtgctcgaa ggccgcgacg gcccgggcgt ctccggccag aggcgcgccg ttccgcgcgt
     7201 cgacgtccct cgctgcgccg gcccggccct cctcgggggg aagggagagt atgtcgaaga
     7261 tgcgcttcag ggagacccgc gcctgctgga tgccgcgtac gttctccgct atggccatga
     7321 gggggtcgaa gaggcgcctt ccgtactcca ggaagacgag gagggtgccg agggtcatgg
     7381 ctcccgcgag gatctccggc gcgaggagct tcacgatgcc cgccatgaag caggggccga
     7441 tgaggaagcc caaggccccc atcgccgagt attcgatcat cgaggccttc acctcgatgt
     7501 cgcgcttgcc cttcgactcc tcgtccaggg cagagacggc ccaggcctcc ctgccgaagg
     7561 cgcgcaggac ctcggagccc tggacgaact cggcgacccg ggcggtgacg gcggcgtagc
     7621 gcttcctgga ggcctcgtag aggacgcgga gcttgtcgaa gaagaagatc acgaaggcca
     7681 gggccaccgg tatggccgcc gcgatgtagg ccgtgatccg cggctccatg gagaagcaga
     7741 ccgcgagcat gccgccgaag aagaggacgt tcgtcgccag ggtgatgccg atcctggaga
     7801 agagctcctt caccttttcc gtgtcgctct ccaccctggc catgagctcg cccacggggt
     7861 gcttgtcgaa gtaggcgacg gggagggtga ggaagtggga gaagaggtcc ttcttgatcc
     7921 gcgtgacgac ctcgaggccg agcctcgcga tcaccatcgt gccgtagtag gtgaggccgc
     7981 ccatgcccac gaccaggccc aggtaggcca gggcgcggag gagcatgccc gcgaggtccc
     8041 gcttcgggat gctctcgtcg atgatgctcc ggaggatgag ggggccgcag agctgccccg
     8101 ccgtgacgag ggagagggcc aggaagccga ggaggaagag gcccttgaag tctccgacgt
     8161 agccggccag gcgccgggag cgggtggatt tcggcatgat aaggaactat agcgcggcgc
     8221 gcggcccgat ttgaagggac gaatctcgct tcattgtttt ttactccttt tcagatattc
     8281 cactgatatt tcagcttgac aggctgacgg aatcgggtct tcaatagttc cggagccaaa
     8341 cgacatgcga agaatcgaac gacatcccat cgtgagcccc gcggccggaa ggcgcgaggt
     8401 ctccttcagc ttcgacgggg agagcctcac gggctacgag ggcgagtccc tctcctcggc
     8461 cctcgccgcc gccggcgtgg tcgccttctc ccgtcaccgg aagggcgagg cgccccaggg
     8521 catcttctgc gccaacggcc aatgctcgca gtgcaccgtc gtcatcgacg gcctcgccaa
     8581 gaaggcctgc gtgacccccc ttgccgccgg catggacgtg aggagcctct ccgggctgcc
     8641 cgtcctcccg gaggacgaca cccccttcgc cggggcggag aagcggagcc tctccgccga
     8701 cgtcctcgtg atcggcggcg gcccctccgg cctctcggcc gcggcggagc tcgcccgtct
     8761 cggcctctcc gtcatcctgg ccgacgacaa ggcctccctg ggcgggaagc tcgtcctcca
     8821 gacccacaag ttcttcggct cggaggagga ctgctacgcg ggcacccgcg gcgtggacat
     8881 agccaagcgc ctggaggagg agctccggag cctcccgaac gtcaccgtcc tcgcgaactc
     8941 ccccgtcgtc gggatctaca aggacaggaa ggccggcgtc tacgtcgact accagcgcta
     9001 cgccctcgtg gacttctccg ccctcgtcgt cgccacgggc gcccgcgaga agagcgtcct
     9061 cttccccggc aacgacctcc ccggcgtcta cggcgccggc gccttccaga ctctcgtgaa
     9121 ccgcgacctc gtgcggccgg cgaggcgggt cctcatcgtg ggctcgggca acgtgggcct
     9181 catcgccgcc taccacgccc tccaggcggg catcgaggtg gcgggcatcg tggacgtcct
     9241 gccgaaggct ggcggctaca aggtccacgc ggacaagatc gtcaggatgg gcgtgcccat
     9301 ttacctcggg acgaccgtcg tcgcggtgga gggctccggc cgcgtggagc gggccacgac
     9361 cgcccgcgtc gactcctcct ataggcccct cctcgagacc gccaggacct acgaggtcga
     9421 caccgtcctc gtggccgccg gcctctcccc ctgcgacgag tactaccgca tggccagggc
     9481 cttcggcttc ctcgccgtca cggcgggcga cgccgaggag atcgccgagg cctcgagcgc
     9541 gatgttcggc gggaagatcg ccgccctctc cctcgcgaag ctcctggaga agaaggtgga
     9601 gatcgacccc tcctgggtgg ccaagcgcga ggtcctcaag tccaggcccg gcgaccgcct
     9661 gccccgcgaa gccgtcgccg cgggagactc ctggaagccg gtcttcttct gcgaggagga
     9721 gatcccctgc aatccctgca cgacggtctg cccgaccaag tccatcaggc tccggccgcg
     9781 caagggcagc ctcctcgacc tgccctactt cgagggcaag gactgccgcg gctgcgcggc
     9841 ctgcgtcgcg gcctgcccgg gcctggccgt ctccctggtc cgccgcatcg acgaggcctg
     9901 gaccgaggtc ctcctgccct acgagttcct cgcggacttc gaggcgggcg ccaggctccc
     9961 cctcctcgac atggagggag ccttcctcga ggaggccgag ctcctgaaga agaccttcta
    10021 caagaagcac aagacctggg tcctctccct caaggtgagc gcggccaacg cgcccagggc
    10081 catcggcgtc agggtccagg ccgagtcggc cacgaggccc ctgcccgagc cgagcttctc
    10141 ccatctcccc gacgaggccg tcgtctgccg ctgcgagcgg atcacggtcg gcgagatcgt
    10201 cgacttcgtg aagaggaacg acgtgcggga cgtgaaccag ctcaagagcc tccgcgtcgg
    10261 catgggcgcc tgcggctcca agacctgctc cgtcctcctg ccgcaggtct tgcgcaaggc
    10321 gggcctggac ccggccgcgg tcgcgccggg ctccctgcgt cccttgggga tggaggtgcc
    10381 catgggcgcc ctcgtcaacg agggcggcta cgacggggga ggggcgggaa gcgaaggcgc
    10441 ggacaggaag ccgcggtcgg ccgccgcggc cgccgccggc gcgagggggg agccgtgagg
    10501 agctacgaca tcgtgatcgc gggggccggc tcgatcggcg tccccctgtc ctactattgc
    10561 gcgaagaagg gcttcaaggt ggccgtcgtc gaaaaggagg cctcctgggg ccgcgggcag
    10621 aaccgcgccg ccatcggcgg gctccgggcg acccactccg accccgcgaa gatcaggatc
    10681 tgccgcgagt ccatcgacat cgtgtcgggg atgaaggagg agctcgggat cgacgtcgag
    10741 tggcgccggg gcggctacct cttcgtcgcc tacgacgagg agcgcgaggc cgccttcagg
    10801 aagctcctcg agatccagaa ggccgccggc ctcgacatcg actgggtcgg acccggacgc
    10861 gtcgccgagc tctcgcccgg catcaacatg aaggacctcc ggggcggcac cttcagcccc
    10921 ggcgacggct acgcctccac cctcatgacc gcgacggcct tccaccggct tgcgatggag
    10981 gccggcgtcg acttcttctt cggcgagacc atccaaggcc tcgaggtctc gggcggaaag
    11041 gtgaagagcc tcaagaccgg gaaggaaagc ttctccgcgc ccctcttcgt caacgccgcc
    11101 ggcgccgacg cggccgacgt ggcggcgatg gcgggcgccg aactccccat tcaccccgac
    11161 tgccacgagg cgggcgtcac cgagcccgtc gagcgcttcc tcgagcccat ggtcgtcgac
    11221 atccgctcgg acgcggagtc gggcaactac tacttctacc aggcggccac cggccaggtc
    11281 gtgttctgca tcacgccccg gccccaggtc tggggcaagg acaaggactc gacctccgcc
    11341 ttcctccccc tctgcgtgcg gaggatgctc gagctctatc cccgcctgcg gaacctcaag
    11401 gtcaggcgga cctggcgcgg catgtacccg atgaccccgg acggcttccc catcgtgggc
    11461 tacccccgcg aggcggacaa cttcctccag gccgtgggca tgtgcggcca gggcttcatg
    11521 atggggccgg gcctggggcg catcctcgcc tccgccatcg cctcggggag ctcccccgcg
    11581 aggccggagg gccagaccga gtacggcttc gtcttcgagg agctcgcccc ctaccgcaag
    11641 ttcgagggca tggagctgct caagtagccc ctcagggagt cagtccgggg gaaggagcct
    11701 tccaggaagg ttccttcctc cgggtccccg tcctccaagg aagcttctca tgcaagcccc
    11761 ggccggcgac gcgaagggtg agtccttgcg ctcctcttgg tttagcatat gctaaactaa
    11821 agggggctca gggatcatca aggccccccg cgaaggaggg tccatgccgc tctccacctt
    11881 cgtcctgggc gagttgtccc gctacgggcc ccttcacggc ctcgagctca agcgccgctt
    11941 cgagaccgcg gggatcgtga gctggggccc cgtcgccgcg acctcagttt accgcgccct
    12001 cgccggactc gagcggaagg gcttggtccg ccggatacag ggccgcttcc gcgccaaccg
    12061 gaagccctac gccgtgactc tggcgggccg ccgggagttc gcctttcgcc tccgcgcgga
    12121 gctcaagtcg ccccgcacct accgcaatcc gctcaacata gccgtggagt acgcgcgcct
    12181 actcccgccc cgcgaactcg cgggcgtgat ggaggagcgc ctcggcgcgc ttcgcggcct
    12241 ggagaggggc gtctccgagc gcctgggcgg cttctcgccg gacgcggctc ccgcgccggg
    12301 cgaggtcctc gccacctgcg agcgcatcct ctggaagacg cgggcggaga tccgcttcgc
    12361 ggtccggatg ctttccctag cccgcgcctc gcccggctag ggcgccgccc cggatcggtg
    12421 gccgaaccgc acccttcgcg ggcgccgctc ccgccgcggg cgcgtcgcgg gggcgcgtcg
    12481 tgggcgcgtc gccgccagcg ccggcccggt cggggcctcg cgcctagagc ccggcccggt
    12541 cggggcctcg cgcctagagc ccgaagagga gggccg
//
LOCUS       DAOOSJ010000048        12518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3366_length_12518_cov_5.681216, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000048 DAOOSJ010000000
VERSION     DAOOSJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3366_length_12518_cov_5.681216"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..1262)
                     /locus_tag="PLG14_03890"
     CDS             complement(363..1262)
                     /locus_tag="PLG14_03890"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOX48304.1"
                     /translation="MILAIDARNRSVSVGFRSGGAWLAKRRFGASSERSADEYVFLLE
                     AAAAALLRSAPSVAAGVAPPGEGAADRAGASLRAGGLPPIEAAWISSVAPSLTPRLAE
                     AVSAAFGVEASVVGPGTRTGLKIRTDQPSEVGSDLVVAAVAARELAGAPSIVVSFGDA
                     VAVSAVNAAGEFVGAAIAPGLETGAASLHQAAALIPEVRLEAPARAIGRNSAESVRAG
                     LVLGYAGLVSRLVALMRAELAADSPGAPLAAVIGAGDAIGRGILAAAGIEGPSGAAGK
                     AGFLFEPDLVLEGLAIIAARNRQ"
     gene            complement(1264..1839)
                     /locus_tag="PLG14_03895"
     CDS             complement(1264..1839)
                     /locus_tag="PLG14_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOX48305.1"
                     /translation="MARESSPVRKAVVAGALGAVSIALFLTPFGYIPWLAGASLTVMH
                     VPAILGAVLEGPVVGMVVGAIFGATSLVKAATAPQGPIDVFFVNPVVSVLPRVLVGLA
                     AWAVYHAFRGKVPALAAAVAGAAGSLANSVLVLGALVAFGALPLAVAGSVLAANGLVE
                     AAAAAVLTLGVVAAWRGVGGKGKARLADEGK"
     gene            complement(1864..2718)
                     /locus_tag="PLG14_03900"
     CDS             complement(1864..2718)
                     /locus_tag="PLG14_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOX48306.1"
                     /translation="MKDLEFFRNLSVGQYVDTGSRLHALGPGTKYLWLLVLCVLAIAA
                     PTPAGAALPFLLALALGKAAGLAPSFLVRGLKPALPVFALAASLQFLFAWPGDASRVL
                     LSLGPVSATWREAWMAVMLVLRTASMIAVVGLFTAVTPESEAARGVEEGLEPLSRLGL
                     PVHRLALAVATAIRFVPIVAGELESIVKAQASRGARFGAGRGGPLAKARAYLPLFVPV
                     TVRALERAELLAEAMEARCYTGEGRSRYARPDNAKGEGAVRWAAFLLGTAALAADALI
                     LEPRIRPF"
     gene            complement(2715..>3654)
                     /locus_tag="PLG14_03905"
     CDS             complement(2715..>3654)
                     /locus_tag="PLG14_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48307.1"
                     /translation="LPPSSAVPAEPAFDLREVSYSYLHGTINETKALSAVSLALSRGM
                     SLALVGRTGSGKSTLLQLLDALAAPASGRVVSLGVEIPSRASRPAPKSAKARRVAEEA
                     VLGLRTRAPLSVQRPETALFERYAADDVAFGPRNLGLSGAPLVERVRRSMEETGLPYA
                     EFRDRPVAALSGGEKRRLAIAGVLALDPEALLLDEPTSALDPPTQLAVLAIVRGRLRS
                     GSTVAMATHSMEEAALADRVAVFSRGRLAAFGPPARLFYEAYDPAWGVARPFAVELAL
                     ALEGLGVPVLSGGERPLSGEDVARAVDALRENGGTP"
     assembly_gap    3655..3754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3755..4479)
                     /locus_tag="PLG14_03910"
     CDS             complement(<3755..4479)
                     /locus_tag="PLG14_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48308.1"
                     /translation="MPLIELESVSYAYPEAERPAVDSVSLSVEAGEYLAVLGANGSGK
                     SSLLRLLNGQRRPASGSVRVAGLDASDPENAHRVRSLLALVFQSPPDQIVSSVVEEDV
                     AFGPENLGLPRGEIGLRIEEALAAAGLSEERRRPTHSLSAGQQQRLAIAGALAMRPAC
                     VAFDEATSMLDPPSRAAVLDIMDGLAARGVAVIHVTHDMAEAARASRVVVLSAGRIAF
                     EGSPEELFAAPASGEPGARPLALA"
     gene            complement(4785..5714)
                     /locus_tag="PLG14_03915"
     CDS             complement(4785..5714)
                     /locus_tag="PLG14_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOX48309.1"
                     /translation="MNEYLDLGSEVAAALAAGKPVVALESTIISHGMPYPQNVETALR
                     VEAAVREAGAVPATIAILGGRLKAGLDESQIRTLGIRGREVVKASRRDLPRLAASAGS
                     ASPIDGATTVAATMIVAALAGIKVFATGGIGGVHRGAERSLDISADLEELAATDVAVV
                     CAGAKSILDLPKTLEYLETKGVPVIGYGTDEFPAFYTPRSGLRLEERADEPAEIARRL
                     KAKWAMGLRGGVVVANPVPESEAMDPLVIDEAIARALAEAEAEGVKGKEVTPFLLAKV
                     KELTGGESLESNIALVLNNARLAARIAAAYSRL"
     gene            complement(5707..6690)
                     /locus_tag="PLG14_03920"
     CDS             complement(5707..6690)
                     /locus_tag="PLG14_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOX48310.1"
                     /translation="MNRSGYVAVVGGINMDIQGRSAGPFRAGDSNPGVAHMSPGGVGR
                     NVAENLVRLGLRTELVAVIGDDPLSAALAESCGRLGIGLRGALRLSQTAASQYICLLD
                     EAGSLVGAVAAMDSIERLTPDRLAERSGLLDGASLIVADANLPEATLAWLAERYGGGG
                     EGLALGSKPPLLALDPVSVAKAGKAKALVGSFSFAKPNRAEAEILSGLPIASRADLPA
                     AAAALRAKGLSDAFISMGGEGLYYEGQGSGGLLARGFVRPPALPVANVSGAGDAALAA
                     LVWGLLSDYDIKERAALAAAAASLAASSLSTVSPEMGAARLLELAKGVVHE"
     gene            6925..7395
                     /locus_tag="PLG14_03925"
     CDS             6925..7395
                     /locus_tag="PLG14_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOX48311.1"
                     /translation="MDQIFERLDRLFKSWVAPEAEETVFGGRRSTGDPDLDDAMSELD
                     DFLDRDRTAAEARERERLAREARARAQSSSSGRPAGPPQALVAAYSTLGLAYGAPFPQ
                     VKAAYKKLLMQHHPDRNGGSAESQKRATELSAKINAAYQRIETWTTTGKLPEEG"
     gene            7476..7913
                     /gene="fliS"
                     /locus_tag="PLG14_03930"
     CDS             7476..7913
                     /gene="fliS"
                     /locus_tag="PLG14_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOX48312.1"
                     /translation="MNANPLAAYKETRVKTASPAQLLVMLYDEALKQCDIAVDYLKGD
                     IKKHPQNIEKVNAALGKVQDILTELMASLDFDAGGEIAQNLFSLYVWFGREILDANLT
                     KEVDRVCAVRAMLSELRGAWAEAGAKMQGGVGSQAPVGVNIAG"
     gene            7930..8412
                     /locus_tag="PLG14_03935"
     CDS             7930..8412
                     /locus_tag="PLG14_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgN"
                     /protein_id="HOX48313.1"
                     /translation="MAVTYNGELERRAAVLRRFRELLVQQRAKFERYLLVLDHERKDI
                     ESGDVDRLVAHVELEEDIVSEIFTFQKVLDPLEDLYRAAYPSGLPAAAGAEDVSEIKT
                     ALVDLRTEVLKRNEENRGLLKARMEVLRAEVLSLRNPLASRKSVYSGGGEGGLLDIKG
                     "
     gene            8520..11414
                     /gene="polA"
                     /locus_tag="PLG14_03940"
     CDS             8520..11414
                     /gene="polA"
                     /locus_tag="PLG14_03940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOX48314.1"
                     /translation="MKSPLYLLDSYAFIYRSYFAFLSRPLRNASGANVSAAFGFFRFL
                     FNLFDERNPGAFAAVFDPKGKNFRHELYPEYKANRQKTPEDLHAQVPLVEEILKALGL
                     PILRAEGYEADDVIATLAERCRAEGRECWIVSGDKDLLQLVGGPVKALRPDSNFAYSP
                     YGPEEVRAEWGVSPERILDYLTLTGDASDNVPGVPGVGDKTAQKLLSEYDGVDEIYAR
                     LAEVKPEGLRRKLEAGRESAELSKRLITLAKDVALPLGGLEELEVGPLDRVAANPLFL
                     REGMRSLAVPTKEAAAKAGPAAGAELFESGASSGAGAGGPAVAAIAPDGVAAALGLGA
                     GQGSAAGPSGSAAPEAAEGLPPELSGEGSYETVATGEALRSWVDRCLAAGAFAFDCET
                     DSLDERAARPVGFSLSYEPRKACYVPLLAPDGPCLAAEAAAAELRRLFAAKEALLVGQ
                     NVKYDYAVMRRFAGPMECRLFDTMVAAWLLDAEAGTYGLEALAERHLGYRGLEFEEVV
                     PKGSTFADVPVEKATRYAAEDADLTYRLYRLFGPRLASAGQEGLFRDLEMPLVPILAG
                     MEEAGIGVEAPELKAYGVELEARLAGIEREIYALVGREFNIASTKQLQEILFLERKLP
                     AGKKTKTGFSTDTSVLEELAGLDPVPALILKHRGLSKLKSTYVEALVRLAEEGRAAAG
                     TAAGGETRIHTHYAQAGTATGRLSSRDPNLQNIPIRDEEGRRIRQAFVASPGRLLVSA
                     DYAQIELVVLAHLSGDPGLERAFREGVDVHRRTASLLFKAAEAEVSPEQRRIAKTINF
                     GVIYGMSAFRLANELKIPRGEAQGFIDAYFATYAGVTDFIRKTVAETEERGYTTTLLG
                     RRRPIPAINSKNKTEKQAAERVAVNTPIQGSAADIIKLAMIRVDRALRAELPRCRLLL
                     QVHDELIVEAPEAEAEAAAALMRREMEAAVVLAVPLRASVEWARSWGDMH"
     gene            11430..12065
                     /gene="coaE"
                     /locus_tag="PLG14_03945"
     CDS             11430..12065
                     /gene="coaE"
                     /locus_tag="PLG14_03945"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOX48315.1"
                     /translation="MLLGLTGGYCAGKNAVAALLEGRGYLCVDVDRLGHEALAGAEGA
                     RAVRARFGEAAIGPDGGVDRKALAAAVFGDPAALADLEAIVHPLVYRLLWALLAPARA
                     EGRDLCINAALLYRMPEAASCDAILEVRAPLPLRLRRGRERDGLPPRRVLERIWSQRS
                     FWRRRREAKRPVVVVRNGGGLDDLEAEVGRALARAESARKAFTAPPRLPIG"
     gene            12125..>12518
                     /locus_tag="PLG14_03950"
     CDS             12125..>12518
                     /locus_tag="PLG14_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48316.1"
                     /translation="MSAESKKLIWISVAVCAFVLVLALAGLFLFAPKQGGGSAPASIG
                     NVAAPKAADPQDYLLAPPPAPSLEAPRSSEGDIIVIYGDKPTELAPPPGAEGGAAAAV
                     PGAAAGANPSAAGVQPAPAASAQPAAKPA"
BASE COUNT         1828 a         4420 c         4648 g         1522 t
ORIGIN      
        1 gatggcgttg tccacgacct ccttgaggag gacgtagatg ccgtcgtcgg ggttggagcc
       61 gtccccgagc cgcccgatgt acatgcccgt cctgaggcgg atgtgctcga gggaggagag
      121 ggtcttgatc ttggactcgt cgtagctctt cgaggatgcc ttggccatga ttggcggaat
      181 tctagcacga taccgcccgg cttgaaaagg cgattcaccg aggaaggagg cgcgccggcc
      241 gggcttggcc ccggcgagcg tcggctgcgg gcgtccgccg cagggcggcg gctacgcgcc
      301 gatgggttcc gggcgcggcc gccgcgggct acggccacgc gccgccgccc gcgcggcgcc
      361 ctctattgcc ggttccgcgc cgcgatgatg gcgagtccct ccaggacgag gtcgggctcg
      421 aaaaggaagc ctgccttccc ggccgcgccg gacggtccct cgatccccgc cgccgcgagg
      481 atgccgcggc ctatggcgtc gcccgcgccg atcaccgccg ccaagggcgc ccccggagag
      541 tcggccgcga gctcggcgcg catgagggcg acgaggcgcg agacgaggcc cgcgtagccg
      601 aggaccaagc cggcccggac cgactcggcg ctgttgcggc cgatggccct ggcgggcgcc
      661 tccagcctga cctcggggat gagggcggcc gcctggtgga gggaggccgc cccggtctcg
      721 aggcccggcg ctatcgccgc gccgacgaac tcgcccgcgg cgttgaccgc ggagacggcg
      781 accgcgtcgc cgaagctgac gacgatcgag ggcgcgcctg cgagttcgcg ggcggccacg
      841 gccgccacga cgaggtcgct cccgacctcg gagggctggt cggtcctgat cttgaggccg
      901 gtccgcgtcc ccggcccgac gacggacgct tccacgccga aggccgcgga cacggcctcg
      961 gcgaggcggg gcgtcaggga gggcgcgacg ctggagatcc aggccgcctc tatcggaggc
     1021 aggccccccg cgcggagcga ggcgccggct cggtcggccg ctccctcgcc cgggggggct
     1081 acgccggccg cgaccgacgg cgcggagcgg agcagggccg cggccgccgc ctcgaggagg
     1141 aagacgtact cgtcggcgct gcgctcggac gaagcgccga agcgccgctt cgcgagccag
     1201 gcgccgcccg agcggaagcc caccgagacg ctccggttcc gcgcgtcgat cgcgaggatc
     1261 acgtcacttg ccctcgtcgg ccaggcgggc cttgcccttg ccgccgacgc cgcgccaggc
     1321 cgcgacgacg cccagggtta ggacggccgc ggcggcggcc tcgacgaggc cgttcgcggc
     1381 caggacggag cccgccacgg cgaggggcag ggcgccgaag gccaccaggg cgcccaggac
     1441 gaggacggag ttcgcgaggg agcccgcggc gccggcgacc gcggccgcga gggccgggac
     1501 cttgcccctg aaggcgtggt agacggccca ggccgcgagg ccgacgagga cgcggggcag
     1561 gacggagacg acggggttca cgaagaagac gtcgatgggg ccctggggcg ccgtcgccgc
     1621 cttgacgagg ctcgtggcgc cgaagatcgc gccgacgacc atgccgacga cggggccctc
     1681 gaggaccgcg cccaggatcg cgggcacgtg catcacggtg agcgatgccc cggcgagcca
     1741 gggtatgtag ccgaagggcg tgaggaagag ggcgatcgag acggcgccga gggcgccggc
     1801 cacgacggcc ttcctgacgg gtgacgattc gcgagccatg ggacctccca tcccggtatc
     1861 cgcctagaag ggtcgaatcc ggggttcgag tattagggcg tccgccgcca gggcggccgt
     1921 cccgaggagg aaggcggccc agcggaccgc cccctcgccc tttgcgttgt cgggccgcgc
     1981 gtagcggctg cgcccctctc ccgtgtagca gcgggcctcc atcgcctcgg cgaggagctc
     2041 agcgcgctcc agggcgcgca cggtgacggg cacgaagagc ggcaggtagg ccctcgcctt
     2101 ggccaggggc cctcccctcc ccgccccgaa gcgcgcgccg cgggaggcct gggccttcac
     2161 gatggactcg agctcgcccg cgacgatggg gacgaagcgg atcgcggtcg cgacggccag
     2221 ggccaggcgg tggacgggga ggcccaggcg ggagaggggc tcaaggccct cctcgacgcc
     2281 gcgggcggcc tcggactcgg gggtgaccgc ggtgaagagg ccgacgaccg cgatcatcga
     2341 ggccgtgcgg aggacgagca tgacggccat ccaggcctcc ctccaggtcg cggagaccgg
     2401 accgagggag aggaggacgc gcgaggcgtc gccgggccag gcgaagagga actggaggga
     2461 ggcggccagg gcgaagaccg gcagggccgg cttgaggccc cggaccagga aggagggcgc
     2521 gaggccggcg gccttcccga gggcgagggc gaggaggaag ggcagcgcgg ccccggccgg
     2581 cgtcggcgct gctatcgcga ggacgcagag gacgaggagc cagaggtact tggtccccgg
     2641 gcccagggcg tggaggcgcg aacccgtgtc gacatactgg ccgacggaga ggttgcggaa
     2701 gaactcgagg tccttcaagg cgtgcctccg ttttcgcgca acgcatccac cgccctggcg
     2761 acgtcctccc cggagagggg cctttcgccg cctgagagga cggggacgcc caggccctcg
     2821 agggcgaggg cgagctcgac ggcgaagggc cgcgcgacgc cccaggccgg gtcgtaggcc
     2881 tcgtagaaga gcctcgcggg cgggccgaag gcggccaggc ggccccggga gaagaccgcg
     2941 acccggtccg cgagggcggc ctcctccatc gagtgcgtcg ccatcgcgac ggtggacccg
     3001 gagcggaggc ggccgcggac gatggcgagg acggcgagct gcgtgggcgg gtctagggcg
     3061 gaggtcggct cgtcgaggag gagggcctcg gggtcgaggg cgaggacgcc ggctatcgcg
     3121 aggcggcgct tctcgcctcc cgagagggcc gcgacggggc ggtcgcggaa ctccgcgtag
     3181 ggaaggcccg tctcctccat ggagcgcctg acgcgctcca cgaggggcgc gcccgagagg
     3241 ccgaggttgc gcggcccgaa ggccacgtcg tcggccgcgt agcgctcgaa gagggcggtc
     3301 tcgggccgct ggacggagag gggggccctg gtcctcaagc cgaggacggc ctcctcggcc
     3361 acgcgccgcg ccttggcgct tttcggggcc gggcgggagg cgcgcgaggg gatctccacg
     3421 ccgagggaga cgacgcgccc ggaggcgggg gccgcgaggg cgtcgaggag ctggaggaga
     3481 gtcgatttgc ccgagcccgt gcgcccgacg agggccaggc tcatgccgcg gctcaaggcg
     3541 agggagacgg ccgagagcgc cttcgtctcg ttgatcgtgc cgtggaggta ggagtaggag
     3601 acctcgcgaa ggtcgaaggc gggctcggcg ggcacggcgg aggaaggcgg aagcnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgagg gccaggggcc gggcgccggg
     3781 ctcgccggag gcgggcgcgg cgaagagctc ctcgggactg ccctcgaagg cgatccgccc
     3841 cgcggagagg acgacgacgc gggaggcgcg ggccgcctcg gccatgtcgt gggtcacgtg
     3901 gatgacggcg acgccgcggg cggccaggcc gtccatgatg tcgaggacgg ccgcgcgcga
     3961 gggcgggtcg agcatcgagg tcgcctcgtc gaaggcgacg caggcgggcc gcatcgcgag
     4021 ggcgcccgcg atcgcgaggc gctgctgctg gccggcggag agggagtggg tggggcggcg
     4081 gcgctcctcg gagaggccgg ctgcggcgag ggcctcctcg atgcggaggc cgatctcccc
     4141 gcggggcagg ccgaggttct cggggccgaa ggccacgtcc tcctccacga cgctggagac
     4201 gatctggtcg ggcggggact ggaagacgag ggcgaggagg gagcggacgc gatgggcgtt
     4261 ctcaggatcg gaggcgtcca ggcccgcgac gcggaccgag cccgaggccg ggcggcgctg
     4321 gccgttgagg agccgcaaga ggctcgactt gcccgagccg ttcgcgccca ggacggcgag
     4381 gtactcgccc gcttccacgg agagcgagac cgaatcgacg gcgggcctct ccgcctccgg
     4441 ataggcgtag gagaccgatt ctagctctat gagcggcatc ggcgctccct tcgtgaggga
     4501 ggccgcgggg atgatcgcat ctacgaacct cgcgtctcgg cggcggaccg tccaagcgtt
     4561 gagtcaaaca tagacaaagc gggcgatttt gtcaattcgc agggcttcga gctcttcgag
     4621 ggacagagct cggtttcgag agacattcga gggacagagc tcggtttcaa gagcctcttc
     4681 gagggacaga gccgcgcggt tatacaaggg cggcgcccga acccttgcgg gtccgggcgg
     4741 gcgtcattcc acgacttggc cctgcccccc gcccaatcca gcgactagag cctggaatag
     4801 gccgcggcga tccgggcggc caggcgggcg ttgttgagga ccagggcgat gttcgactcg
     4861 aggctctcgc ccccagtgag ctccttcacc ttggcgagga ggaagggcgt cacctccttg
     4921 cccttgacgc cctcggcctc ggcctccgcg agggcccggg ctatggcttc gtcgatgacg
     4981 agggggtcca tagcctcgga ctcggggacg gggttcgcga cgacgacgcc cccgcggagg
     5041 cccatggccc acttggcctt gaggcggcgg gctatctcgg cgggctcgtc ggcccgctcc
     5101 tccaggcgga ggcccgagcg gggcgtgtag aaggcgggga actcgtcggt gccgtagccg
     5161 atgacgggca cgcccttcgt ctccaggtac tcgagggtct tcggcaggtc gaggatggac
     5221 ttggcccccg cgcagacgac ggcgacatcg gtcgccgcga gctcctcgag gtcggcggag
     5281 atgtcgaggc tccgctcggc gccgcggtgg acgccgccga tgccgccggt cgcgaagacc
     5341 ttgatgccgg ccagggccgc gacgatcatc gtggcggcca cggtcgtggc gccgtcgatc
     5401 ggggaggccg agccggcgga ggcggcgagg cgcggcaggt cgcggcggct cgccttgacg
     5461 acttcgcgac cgcggatgcc cagggtgcgg atctgggact cgtcgaggcc ggccttgagg
     5521 cggccgccga ggatggcgat ggtggcgggg acggcgccgg cctcgcggac ggccgcctcc
     5581 acgcgcaggg ccgtctccac gttctgcgga tagggcatgc cgtgggagat gatggtcgac
     5641 tccagggcga cgacgggctt gccggcggcg agggccgcgg cgacttccga tccgaggtcc
     5701 aggtactcat tcatggacga ctcctttcgc gagctcgagg aggcgcgcgg cccccatctc
     5761 tgggctcacc gtcgagaggg acgaggcggc cagggaggcg gccgcggccg ccagggccgc
     5821 ccgctccttg atgtcgtagt cggagaggag gccccagacc agggccgcga gggccgcgtc
     5881 gcccgcgccc gagacattgg cgacggggag ggcggggggc cggacgaagc cccgcgcgag
     5941 gaggccgcct gagccctgcc cctcgtagta gagtccctcg ccgcccatgg agatgaaggc
     6001 gtcggagagg cccttggcgc gcagggcggc ggccgcggcg gggaggtcgg cgcgggaggc
     6061 gatggggagg ccggagagga tctcggcctc cgcgcggttc ggcttcgcga aggagaagct
     6121 cccgaccagg gccttggcct tgccggcctt ggcgacggag acgggatcga gggcgaggag
     6181 ggggggcttg gagccgaggg cgagtccctc ccctcccccg ccgtagcgct cggcgagcca
     6241 ggccagggtg gcctcgggaa ggttcgcgtc ggcgacgatg agggaggcgc cgtcgaggag
     6301 gccggagcgc tcggccaggc gatcgggcgt gaggcgctcg atggagtcca tcgcggctac
     6361 ggcgcccacg aggctccccg cctcgtcgag gaggcagatg tactgggagg cggcggtctg
     6421 ggagaggcgc agggcgcccc ggaggccgat gccgaggcgg ccgcaggact ccgcgagggc
     6481 ggccgagagg ggatcgtccc cgatgacggc gacgagctcg gtgcggaggc ccaggcggac
     6541 caggttctcg gcgacgttcc tgccgacgcc gcccggactc atgtgggcaa cgccgggatt
     6601 ggagtccccg gcccggaagg gccccgcgga gcgtccctgg atgtccatgt tgatgccgcc
     6661 cacgacggcg acgtagccgc tccggttcat gttcctcctg ccggaaagcc ccgaacgggg
     6721 ctcccgggag ccccgttcgg ggccggagta gagccataca ccgggaaggc cgtccgcggc
     6781 aaggaccgcg ggggttttcc ggaccggcga agccggcggt cgcgccgggg gccgtccggg
     6841 gacagatccg gatccgggga agggcggccg acttgaacag gcccccgcga tggggtaaag
     6901 tggagattct aaggggtcag catcgtggat cagatctttg agcgtctcga ccgcctcttc
     6961 aagtcctggg tggctcccga agccgaggag accgtcttcg gcggccgccg ctccacgggc
     7021 gatcccgacc tggacgacgc catgagcgag ctcgacgact tcctggaccg ggacaggacg
     7081 gccgccgagg cccgcgaacg ggagcgcctg gcccgcgagg ccagggcccg cgcccaatcc
     7141 tcctcctccg gaaggccggc gggccctccc caggccctgg tcgccgccta ctcgaccctc
     7201 ggcctggcct acggagcgcc cttcccgcag gtgaaggccg cctacaagaa gctcctcatg
     7261 cagcaccatc ccgaccgcaa cggcggcagc gccgaaagcc agaagcgcgc caccgagctc
     7321 tccgcgaaga tcaacgctgc ctaccagcgc atcgagacct ggacgacgac gggaaagctt
     7381 cccgaggaag gctgagccgg cggccgtttc ccctcaagag agggggggcg aaggccgaga
     7441 atcgagctag acccaaaggg gagggaagga ccggaatgaa cgccaatccg ctcgcagcct
     7501 acaaggagac tcgcgtgaag accgcgagcc ccgcccagct cctcgtcatg ctctacgacg
     7561 aggccttgaa gcagtgcgac atcgccgtcg actacttgaa gggcgacatc aagaagcatc
     7621 cccagaacat cgagaaggtc aacgcggccc tgggcaaggt ccaggacatc ctcaccgagc
     7681 tcatggccag cctggacttc gacgccggcg gagagatcgc ccagaacctc ttctccctct
     7741 acgtctggtt cggccgcgag atcctcgacg cgaacctcac gaaggaagtc gaccgcgtct
     7801 gcgccgtcag ggccatgctc tcggagctcc gcggcgcctg ggccgaggcc ggcgcgaaga
     7861 tgcagggcgg cgtcggctcc caggcccccg tcggcgtcaa catcgcgggc tagggccgga
     7921 gacggcgagg tggcggtgac ctacaacggc gagctcgagc ggcgggccgc cgtgctccgc
     7981 aggttccgcg agctcctggt ccagcagcgg gccaagttcg agcgctacct cctcgtcctg
     8041 gaccacgagc ggaaggacat cgagtcgggc gacgtggacc gcctcgtcgc ccacgtggag
     8101 ctcgaggagg acatcgtctc cgagatcttc accttccaga aggtcctcga tcccctggag
     8161 gacctctacc gcgccgccta cccctcgggc ctccccgccg cggcgggagc cgaggacgtc
     8221 tccgagatca agaccgccct cgtcgacctc aggaccgagg tcctgaagcg caacgaggag
     8281 aaccgcggcc tcctcaaggc ccgcatggag gtcctgcgcg ccgaggtcct ctccctccgc
     8341 aaccccctcg cctcccgcaa gtccgtctac tcgggcggcg gcgagggcgg cctcctcgac
     8401 atcaagggct agagcagggc ccagggccgg ggccggcgtc ccgcccgggg ccgcccgagc
     8461 cgcctttccg gaccgcggcc gccattgcgc gcccgggcca aagccgctat ccttcccgca
     8521 tgaagagccc cctctacctc ctcgactcct acgccttcat ctaccgctcc tatttcgcct
     8581 tcctctcgcg gcccctccgc aacgcctcgg gcgccaacgt ctccgcggcc ttcggctttt
     8641 tccgcttcct cttcaacctc ttcgacgagc ggaaccccgg cgccttcgcc gccgtattcg
     8701 acccgaaggg caagaacttc cgccacgagc tctatcccga gtacaaggcc aaccggcaga
     8761 agacgcccga ggacctccac gcccaggtcc ccctcgtcga ggagatcctc aaggccctgg
     8821 gcctccccat cctccgcgcc gagggctacg aggccgatga cgtcatcgcc accctggccg
     8881 agcgctgccg cgccgagggc cgcgagtgct ggatcgtctc cggcgacaag gacctcctcc
     8941 agctcgtcgg cgggcccgtg aaggccctgc gccccgactc gaacttcgcc tacagcccct
     9001 acgggcccga ggaggtgcgg gccgagtggg gcgtcagccc cgagcgcatc ctcgactacc
     9061 tcaccctgac gggcgacgcc tccgacaacg tgcccggcgt gccgggcgtc ggcgacaaga
     9121 ccgcgcagaa gctcctttcc gagtacgacg gcgtcgacga gatctacgcc cgcctcgccg
     9181 aggtgaagcc cgagggcctg cgcaggaagc tcgaggcggg cagggagagc gccgagctct
     9241 cgaagcgcct catcaccctc gccaaggacg tggccctccc cttgggcggc ctcgaggagc
     9301 tcgaggtcgg gcccctggac cgcgtcgcgg ccaatcccct cttcctccgc gaggggatgc
     9361 ggagcctcgc cgtgccgacc aaggaggcgg ccgcgaaggc cggcccggcc gccggcgccg
     9421 agctcttcga gtcgggcgcc tcgagcgggg ccggcgcggg cggcccggcc gtcgcggcca
     9481 tcgcgcccga cggcgtcgcg gccgcgctgg gcctgggcgc gggacaaggg tccgccgccg
     9541 gcccctcggg ctccgccgcg cccgaggccg ccgagggcct cccgcccgag ctctcgggcg
     9601 agggctccta cgagaccgtc gcgacgggcg aggccttgcg ttcctgggtg gaccgctgcc
     9661 tggccgccgg ggccttcgcc ttcgactgcg agaccgactc cctggacgag cgcgcggcga
     9721 ggcccgtcgg cttctccctc tcctatgagc cgcgcaaggc ctgctatgtc cccctcctcg
     9781 cgcccgacgg cccctgcctc gccgccgagg cggcggccgc cgagctcagg cgcctcttcg
     9841 ccgccaagga ggccctcctc gtcggccaga acgtcaagta cgactacgcg gtgatgcggc
     9901 gcttcgcggg gccgatggag tgccgcctct tcgacacgat ggtcgccgcc tggctcctcg
     9961 acgcggaggc cgggacctat ggcctcgagg ccctggccga gcggcacctc gggtaccggg
    10021 gcctcgagtt cgaggaggtc gtccccaagg gctccacctt cgcggacgtc cccgtggaga
    10081 aggcgacgcg ctacgcggcg gaggacgccg acctcaccta caggctctac aggctcttcg
    10141 ggccccgcct cgcctcggcc gggcaggagg gcctcttccg cgacctggag atgcccctcg
    10201 tccccatcct cgcgggcatg gaggaggcgg ggatcggggt cgaggcgccg gagctcaagg
    10261 cctacggggt cgagctcgag gcgaggctcg ccgggatcga gcgcgagatc tacgccctgg
    10321 tcggccgcga gttcaacatc gcctccacga agcagctcca ggagatcctc ttcctggaac
    10381 ggaagctgcc cgcgggcaag aagaccaaga cgggcttctc cacggacacg agcgtcctcg
    10441 aggagctcgc cggcctggat cccgtgcctg ccctcatcct caagcaccgc ggcctctcca
    10501 agctcaagag cacctacgtc gaggccctgg tgcggctggc cgaggagggc cgggccgcgg
    10561 ccggaaccgc ggcgggtggc gagacccgca tccacacgca ctacgcccag gcggggacgg
    10621 cgacgggccg cctctccagc cgcgacccca acctccagaa catcccgatc cgcgacgagg
    10681 agggcaggcg catcaggcag gccttcgtcg cctccccggg gaggctcctc gtctccgccg
    10741 actacgcgca gatcgagctc gtggtcctgg cccacctctc gggcgacccg ggcctggagc
    10801 gcgccttccg cgaaggcgtc gacgtccacc gccgcacggc gagcctcctc ttcaaggccg
    10861 ccgaggccga ggtgagcccc gagcagcgcc ggatcgcgaa gaccatcaac ttcggcgtca
    10921 tctacgggat gagcgccttc aggctcgcga acgagctcaa gatcccccgc ggcgaggccc
    10981 aaggcttcat cgacgcctac ttcgccacct acgccggggt cacggacttc atccggaaga
    11041 ccgtcgcgga gacggaggag cggggctaca cgacgaccct cctggggcga aggcgcccca
    11101 tccccgcgat caactccaag aacaagacgg agaagcaggc cgccgagcgc gtggcggtca
    11161 acacgcccat ccagggcagc gccgccgaca tcatcaagct cgcgatgatc cgcgtggacc
    11221 gcgccctgcg cgcggagctc ccccgctgcc gcctcctcct ccaggtccac gacgagctca
    11281 tcgtcgaggc gccggaggcc gaggccgagg ccgcggccgc actcatgcgc cgcgagatgg
    11341 aggccgccgt cgtcctcgcc gtccccttga gggcgagcgt ggaatgggcc cgctcctggg
    11401 gcgacatgca ctaggcggga ggggaagcga tgctgctcgg actcaccgga ggctactgcg
    11461 ccggaaagaa cgcggtcgcc gccctcctgg aggggcgggg ctacctctgc gtcgacgtgg
    11521 accgcctggg ccacgaggcc ctcgcgggcg cggagggcgc ccgggcggtg cgggcccgct
    11581 tcggcgaggc cgcgatcggc ccggacggcg gggtcgacag gaaggccctc gcggcggccg
    11641 tcttcggcga ccccgcggcc ctggccgacc tggaggccat cgtccacccc ctcgtctacc
    11701 gcctcctctg ggccctcctg gccccggccc gggccgaggg ccgcgacctc tgcatcaacg
    11761 ccgccctcct ctaccggatg ccggaggcgg cctcctgcga cgccatcctg gaggtgcggg
    11821 ctcccctccc cctgcgcctc cgccgcggcc gcgagcggga cggccttccg ccccgccgcg
    11881 tcctggagcg gatctggagc cagcggagct tctggcgccg ccgccgcgag gcgaagaggc
    11941 ccgtcgtcgt cgtccgcaac ggcggcggcc tcgatgacct ggaggccgag gtcgggcgcg
    12001 cgctcgcccg ggccgagtcg gcgcgaaagg cctttaccgc ccctccccga ttgccgatag
    12061 ggtaaatgga atagagcgcc gtcgcctccg ccttcggagg gggcgctccc gggaggggct
    12121 cgtcatgtcc gccgaaagca agaagctcat ctggatatcg gtcgccgtct gcgcctttgt
    12181 cctcgtcctg gccctcgccg gcctcttcct cttcgccccc aagcagggcg gggggtccgc
    12241 gcccgccagc ataggcaacg tggcggctcc caaggccgcc gatccccagg actacctcct
    12301 cgcccctccc ccggccccga gcctggaggc cccgaggagc tccgagggcg acatcatcgt
    12361 gatctacggc gacaagccga cggagctcgc ccctcccccg ggcgccgagg gcggagccgc
    12421 ggccgccgtc ccgggcgccg ccgcaggcgc caacccctcc gcggcgggag tccagccggc
    12481 tcccgcggcg tccgcgcagc cggccgccaa gcccgccg
//
LOCUS       DAOOSJ010000049        12408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3392_length_12408_cov_6.236461, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000049 DAOOSJ010000000
VERSION     DAOOSJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_12408_cov_6.236461"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /gene="dnaE"
                     /locus_tag="PLG14_03955"
     CDS             complement(<1..2177)
                     /gene="dnaE"
                     /locus_tag="PLG14_03955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX48317.1"
                     /translation="MADFVHLHNHSDYSLLDGAAGVGAMVAKAKALGMPGLALTDHGN
                     MFGAVKFYDACKDAGVNPIVGSEFYVAGGSRREKTGTEGGNKYHHLVLLAQDAEGYKN
                     LLKLSSASYTEGFYYKPRIDDEILAAHAKGLIAGSACLGGEIPGLILRGKEAEAERKA
                     LWYAELFGKDRFYLELQDHGIPEQKPVNKALVAMARRTGLPLVATNDMHYLDRSDAEA
                     HDVLLCIGTNRKLAEPGRMRFHGPEFYMKTAEEMAAIFGEVPEALSNTLRVNEMVRLD
                     LAYPGPLLPDYEIPPEFSGPEDYLRHLTYEGLKRRYPDGGPEVRARAEYELDVIIKMQ
                     FTGYFLIVWDFINWSKEHGIPVGPGRGSGAGSIVAYSLRITDIDPIRYKLLFERFLNP
                     ERISMPDFDIDFCFERRGEVIEYVTRKYGADRVGQIVTFGTLKAKAVIKDVARALDIS
                     FADSNEIAKLVPEDPKMTLKKAFELEPKLKALAEDQRYKGLFEVATRLENRNRHTSFH
                     AAGIVIGKSELTDYVPLFKDPKTGIVATQFTMDYLEKCGLVKMDFLGLKTLTLIKNTI
                     DLVRRRGVEIEESAIPEDDAKTFELLGEGKSTSVFQFESSGMQGILKQAKPSCMEDLI
                     ALNALYRPGPMAYIPQFIDSKWGRQAIAYPDPCLEEILKETYGVIVYQEQVMQVAQKI
                     GGYSLGQADLLRRAMGKKKVEVMVKEKEKFVKSAVANGFKAEDA"
     gene            2402..2992
                     /locus_tag="PLG14_03960"
     CDS             2402..2992
                     /locus_tag="PLG14_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOX48318.1"
                     /translation="MSYPTPAHLERINIQPGERRASAKPVLLSTLLGSCVAVCLYDEK
                     TGIAGLNHFLLAAPRYAKDMPMTHTEAGRYGINAMELLINDMVGLGASRQRLKAKAFG
                     GATVIGGARDNFFCVHEVNQRFIREYLGTEGIPLVAEDLGGDRGRLVYFHTDTFQVFR
                     RYIQRSQLGRIEEDERLFWKAKVEKPEAEEGKVILF"
     gene            3135..4835
                     /locus_tag="PLG14_03965"
     CDS             3135..4835
                     /locus_tag="PLG14_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48319.1"
                     /translation="MSSSDSAGKSAGTEGTEPLLSEAEALLRSDPASARELATAIAVD
                     AELAADRPLAAYALCLSGAASLHLGDIAVGLGDLERSRRLASGGGASPALRIRIANGI
                     GSALHQLGRYAEADRAFERALQSARRLGQPSGVLRCLVNYGFAKSEAGRHEDALAHFT
                     EALDLSLEASGGDYERAEALPVLLGNIGLALLRLGRVEDARSYLGRAVEASAARGDQV
                     GLANCLCTLGESHLGENPDEAEELLAESLSTCLRIGANMTGVRSALVLARHSLDSGQL
                     DEAEAVIAEWLPRAERGDSLRHAELVRLKGRLLSLRGRFSEAYEASELAGALERKVGF
                     ASAATASLAIDRGKYARRSKRLVERVESWNEAVNLTLAALIEAKDAPTGRHVERAAEI
                     ARRLGERLIASAAIPGLSEALLDAIVGSTPLHDIGKIAVPDSIIGKPGPLDSGERERM
                     QRHVVVGKEILLDAAMRVSFEPRIKVAAEIAGYHHERWDGTGYPEGRSGDAIPISARI
                     VAVADVYDAIRSERPYKPAHGREAAASYVRDNASLHFDPRVVAAFLDIEDEIAELYAS
                     "
     gene            complement(4959..6359)
                     /locus_tag="PLG14_03970"
     CDS             complement(4959..6359)
                     /locus_tag="PLG14_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX48320.1"
                     /translation="MRVLIVDDERNIRESLGRLLALEGIESATAPDGEAGAALLSEEA
                     FDAVVLDLKMPGMGGQGLLEWMRAEGLRTPAVMISALGEIGDAVNALKTGASDYLVKP
                     FDPAELVARLKSLVAARRREDLVEAGARTRPSGGGLLGESRAMRDIRRLVGKVAARDA
                     TVLVTGESGTGKEVVARAIHEASPARDEPFVAVNLGALPLELAESELFGYEKGAFTGA
                     DDRKPGLFELAGSGILFLDEVGEMPLSLQVKLLRALQERKTRRLGGTRDIPVRARLVA
                     ATNRDLKAAVAEGRFREDLFYRLDVVRVEVPPLRERAEDIPLLAGHILGKIAARSGRP
                     AKRLAGEALAALSAYRFPGNVRELENVLERAAILCEGEEILLGELGLPEGGPAQPGAR
                     GAPRGGPGGAAGNGGGAGRPGALGRQGGSLESLERDAVAAALEKWGGNRTKAALELGV
                     SRRTILNKIKLYGLEG"
     gene            complement(6356..7864)
                     /locus_tag="PLG14_03975"
     CDS             complement(6356..7864)
                     /locus_tag="PLG14_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX48321.1"
                     /translation="MMKPMKLRHLSLVLALLAFAGATAVDVAIFRGMSARNRLESRND
                     AERRMNILLSSLRDYPDFGAAIEALPSLRGKVLGVAAFDAEGRTLYSFGKMPELGASA
                     KELAAELEGGRPGDAAAGQASSAEESAAAAKAAAAAASAGLDGDGRIYRENPADSSLV
                     ILLDPKPKGPPPPRRDGGRTDGRPEDQPKDGDEGREPSFFFSTLRDAELLALEVEAGE
                     YWAAERFHRFLFPAAELLLACLVVLVRGLVLRNAEYRKRIEEQRSLVMLGTAASTIAH
                     EIKNPLLSIKLQTSILLRTCPESASRELRIIDDEVARLSALSGRVSDYLRDPAGRPVP
                     VDAAEVASSVSLRRLGRDIVAVRGKAAVLADPARLRSVLENLLENAIESGGPEEELAV
                     EVGAAEGEARIDVLDRGSGIAPGDRERVFDPFYTTKSRGTGIGLSVCRRFSEAAGGGI
                     SLEERQGGGTRARLVLPLAAAAPPGGEARGGETRLRDERRAAGDGVPGGGAP"
     gene            complement(7879..9312)
                     /locus_tag="PLG14_03980"
     CDS             complement(7879..9312)
                     /locus_tag="PLG14_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX48322.1"
                     /translation="MRPNRLAALALGFGLALGSALGAAALPLEAPGQPAASAAAAALA
                     AAPASTDAAAENAASASAPLGLEEAKSLALARSAAVRQALLAVDEAVLAERLESYSFL
                     PSLSVDAGGKYAYGSSASGAVAASAGVGIAQPLYDGGRSALRKAIDAAATGMARESVR
                     SAVSSLEAEVESAYFGVLEAEAALEAAKSDLESSSLHLGLATAKYEASALSRPDLLEV
                     ESEAATAEAAFVKARREVSLSRAALASLTGLPSGTALAGVDAARYDQAARGLALASDE
                     AVSAAASRLAAAAAESDPETASAALASGKAAASVKLAERAWLPGVSAGYSHTVPLGAG
                     ESLGLGSGSLSVSASLSLDFWTKKAGLESARLAAGKAAIGLEEAKRKAALGVESAAYD
                     CAALARSLVASAKALEHAESRLEAVRELFALSAASSAELSDAEALAGSARKALVAARF
                     GFISSLSGLGRLAGLEDEELLIAALRG"
     gene            complement(9323..10513)
                     /locus_tag="PLG14_03985"
     CDS             complement(9323..10513)
                     /locus_tag="PLG14_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48323.1"
                     /translation="MTASKLAVLSARSIFRNRMRSILTSLGIIIGVCSVIVMVAVGEG
                     SQAQIKARISSMGTNLIMVMPPRGPREANRLSTADVKRLRAESSSLAAISGTVRLSAK
                     VVGGSGYYETSVQGVEPSYLEIKGWGVESGDFFSEADLASRSKVAVLGATVAKELFGT
                     DDPLGERVRIGATPFTVVGVLSKKGSSGMGDDQDDVVMVPLDTALTRLNKDRYINSIE
                     ASAVGEEFMDAAQAEVESILREAHRIQPGAEADFDVMNQAEIIATASETSKTLTTLLA
                     AIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMSVGARKRDILLQFLAESVILSLLGG
                     LVGILLAVLVCVVLGSAGIPAIVNPAVVATSAAFAAAVGVFFGYYPARKASNLYPIDA
                     LRYE"
     gene            complement(10510..11289)
                     /locus_tag="PLG14_03990"
     CDS             complement(10510..11289)
                     /locus_tag="PLG14_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48324.1"
                     /translation="MSAVIELSGIKRHYEVGEVVVKALRGVDLRVEQGEFVAVMGPSG
                     SGKSTLMNVLGCLDKPTEGIYLLDGVDASKAGADQFAEIRNRKIGFVFQGFNLLSRTT
                     ALENVELPLFYARGGARGARRGDGEERARRALEEVGLGGRIRHKPSQLSGGQQQRVAI
                     ARAIVNDPAFILADEPTGNLDTEMTLEVMSLFQDLNGRGKTIVMVTHEPEVAAYTRRV
                     IAMRDGAIVSDEPVRARREAAADLAAWKERNALLSGGRRVS"
     gene            complement(11286..>12408)
                     /locus_tag="PLG14_03995"
     CDS             complement(11286..>12408)
                     /locus_tag="PLG14_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX48325.1"
                     /translation="TLLNTDLLELQRKEALAEVRKARAAYELQALDERNKAKLFEKGL
                     VSDYENKTSKATLESCAASLASAESALEVIELKLGSYARVVSPIDGIVLDRNVDVGQG
                     VVEGSSSNSSSLFTLAKDLSRMEIKAEVDELDIASIKVGQEARFTVEACPGLSFKGSV
                     REIRLVPESSDNVVSYCVMVAADNKEGRLFPGMTADIEFVKESREGVLVVPNAALKFT
                     PPGLSEEEVARLVFAAGLSGLGEAERAEALSRYDEAAKAAKDAAAASGGQAQAGGLAG
                     MVMQGPGPGGPPGMRGRATAKAGSSGQAAGFAAGAAGPEAGGLSGAAAELKRLWYLDG
                     SGAFGVILVEAGASDGTYTEVSSDAELEGRKVVLKARAE"
BASE COUNT         1720 a         4467 c         4390 g         1831 t
ORIGIN      
        1 gcgtcctcgg ccttgaagcc gttcgcgacc gcgctcttca cgaacttctc cttctccttg
       61 accatgacct cgaccttctt cttgcccatc gcgcggcgga ggaggtcggc ctggcccagg
      121 ctgtagccgc cgatcttctg ggcgacctgc atgacctgct cctggtagac gatgacgccg
      181 taggtctcct tgaggatctc ctccaggcag gggtcgggat aggcgatggc ctgcctgccc
      241 cacttcgagt cgatgaactg gggtatgtag gccatggggc ccgggcggta gagggcgttc
      301 agggctatga ggtcctccat gcaggagggc ttggcctgct tgaggatgcc ctgcatgccc
      361 gaggactcga actggaagac gctcgtgctc ttgccctcgc ccaggagctc gaaggtcttc
      421 gcgtcgtcct ccggaatcgc ggactcctcg atctcgacgc cgcgcctcct gacgaggtcg
      481 atcgtgttct tgatgagggt gagggtcttg aggcccagga agtccatctt cacgaggccg
      541 cacttctcca ggtagtccat cgtgaactgc gtcgcgacga tgcccgtctt agggtccttg
      601 aagagcggca cgtagtcggt gagctcgctc ttcccgatga cgatgcccgc ggcgtggaag
      661 ctcgtgtggc ggttccggtt ctccaggcgc gtggcgacct cgaagaggcc cttgtagcgc
      721 tggtcctccg ccagggcctt gagcttgggc tcgagctcga aggccttctt gagcgtcatc
      781 ttcgggtcct cgggcacgag cttcgcgatc tcgttcgagt cggcgaagct gatgtccagg
      841 gcccgcgcca cgtccttgat gacggccttg gccttcaggg tcccgaaggt gacgatctgg
      901 ccgacgcggt cggcgccgta cttcctcgtc acgtactcga tgacctcgcc ccggcgctcg
      961 aagcagaagt cgatgtcgaa gtcaggcatg gagatgcgct cggggttgag gaagcgctcg
     1021 aagaggagct tgtaccggat cgggtcgatg tccgtgatgc gcagggagta ggcgacgatg
     1081 gagcccgcgc ccgagccgcg gccggggccg acggggatgc cgtgctcctt ggaccagttg
     1141 atgaagtccc agacgatgag gaagtagccc gtgaactgca tcttgatgat gacgtcgagc
     1201 tcgtactcgg cgcgggccct gacctcgggg ccgccgtccg gatagcggcg cttgaggccc
     1261 tcgtaggtca ggtggcggag gtagtcctcg gggccggaga attccggggg gatctcgtag
     1321 tcgggaagga gggggccggg ataggccagg tcgagcctga ccatctcgtt caccctgagg
     1381 gtgttcgaga gggcctcggg cacctccccg aagatggcgg ccatctcctc ggccgtcttc
     1441 atgtagaact ccgggccgtg gaagcgcatg cgccccggct ccgcgagctt gcggttcgtc
     1501 ccgatgcaga gcaggacgtc gtgggcctcg gcgtcggagc ggtccaggta gtgcatgtcg
     1561 ttggtcgcga cgagggggag gcccgtacgc ctcgccatcg cgaccagggc cttgttgacg
     1621 ggcttctgct cggggatgcc gtggtcctgg agctcgaggt agaagcggtc cttcccgaag
     1681 agctccgcgt accagagggc cttcctctcg gcctccgcct ccttgcccct gaggatgagg
     1741 cccgggatct cgccgcccag gcaggccgag cccgcgatga ggcccttcgc gtgggccgcc
     1801 aggatctcgt cgtcgatccg cggcttgtag tagaaaccct ccgtgtagga ggccgaggag
     1861 agcttgagga ggttcttgta gccctccgcg tcctgcgcga ggaggaccag gtggtggtac
     1921 ttgttgccgc cctcggtgcc cgtcttctcg cgcctcgagc cgcccgcgac gtagaactcc
     1981 gagccgacta tcgggttgac gcccgcgtcc ttgcaggcgt cgtagaactt cacggcgccg
     2041 aacatgttgc cgtggtcggt cagggccagg cccggcatgc cgagggcctt ggccttggcg
     2101 accatggcgc ctacgcccgc cgcgccgtcg aggagggagt agtctgagtg gttgtggagg
     2161 tggacgaagt cggccatggc ggggagtata gcctccgggc cggggagcga gaaaggggcc
     2221 ccgcgggccg ccggcggcgc cgcccctccc cgccgccccg gcctgccgcg gcgggcgcgg
     2281 acgcggcggc ctacgacccg ccggccatgg cccaggcgtt ttttcgggcc gggaggcctc
     2341 ccattcgggc atgccgtggc tatacttaag cggggggaag gccttcccct cggaggctcg
     2401 cttgtcctac ccgacgccgg cgcaccttga gagaatcaac atccagcccg gggagcggcg
     2461 cgcctcggcc aagcccgtcc tcctctcgac cctcctcgga tcctgcgtgg ccgtctgcct
     2521 ctacgacgag aagaccggga tagccggcct caaccacttc ctcctcgcgg ccccccgcta
     2581 cgccaaggac atgcccatga cccacacgga ggccgggcgc tacgggatca acgcgatgga
     2641 gcttctcatc aacgacatgg tgggcctggg cgcctcccgc cagcgcctca aggccaaggc
     2701 cttcggcggc gcgaccgtca tcggcggcgc ccgcgacaac ttcttctgcg tccacgaggt
     2761 caaccagcgc ttcatccggg agtacctcgg gacagagggc atccccctcg tcgccgagga
     2821 cctgggcggg gaccgaggga ggctcgtcta cttccacacc gacaccttcc aggtcttcag
     2881 gcgctacatc cagcggagcc agctcggcag gatcgaggag gacgagcgcc tcttctggaa
     2941 ggccaaggtc gagaagcccg aggccgagga aggcaaggtc atcctcttct aggccggccc
     3001 tcggccccgc ggcgccctct gtgttgccgg ccgccgcggc ggcggcttcc gcggcggcgg
     3061 cttcagtagc ggtggaggcc tccgcggcgg cgcgtgattg agccctgcgg ccattccggt
     3121 gtaggatgag gcctatgtcc tccagcgatt ctgccggcaa gagcgccgga accgagggta
     3181 ccgagcccct cctttcggag gccgaggcgc tgctccgcag cgacccggcc tccgcccgcg
     3241 agctcgccac ggccatcgcc gtcgacgccg agctcgccgc cgacaggccc ctggccgcct
     3301 acgccctctg cctctcaggc gccgcctccc tgcacctggg cgacatcgcc gtgggcctcg
     3361 gcgacctgga gcggagccgc cgcctcgcct cgggcggcgg ggcgagcccc gccctgagga
     3421 tcaggatcgc gaacggcatc ggctcggccc tccaccagct cgggcgctac gcggaggccg
     3481 accgcgcctt cgagcgggcc ctccagtcgg cgcgccgcct gggccagccg agcggcgtcc
     3541 tgcgctgcct cgtgaactac ggcttcgcga agtcggaggc gggccggcac gaggacgccc
     3601 tggcccattt caccgaggcg ctcgacctct ccctcgaggc ctccggcggc gactacgaga
     3661 gggccgaggc cctgcccgtc ctcctcggca acatcggcct cgccctcctc aggctcggcc
     3721 gcgtcgagga cgcccgctcc tacctgggcc gggccgtcga ggcctcggcc gcccgcgggg
     3781 accaggtcgg gctcgcgaac tgcctctgca ccctcgggga gtcccatctg ggcgagaacc
     3841 ccgacgaggc cgaggagctc ctcgccgagt ccctgtccac ctgcctgcgc atcggcgcga
     3901 acatgaccgg ggtccgctcc gcgctggtcc tagcccgcca ctccctagac tcgggccagc
     3961 tcgacgaggc ggaggccgtc atcgccgagt ggctgccccg cgcggagcgc ggcgactccc
     4021 tccgccacgc cgagctcgtc cggctgaaag gacgcctcct ctccctgcga ggccgcttca
     4081 gcgaggccta cgaggcgagc gagctcgcgg gcgcgctcga gcgcaaggtc ggcttcgcct
     4141 ccgccgcgac ggcctcgctc gccatcgacc gcggcaagta cgccaggcgc tcgaagcgcc
     4201 tcgtcgagcg ggtcgagagc tggaacgagg cggtcaacct cacgctcgcg gccctcatcg
     4261 aggcgaagga cgccccgacg ggccgccacg tggagcgggc ggcggagatc gcgcggcgcc
     4321 tgggcgagcg gctcatcgcc tccgcggcca tacccggcct gagcgaggcc ctcctggacg
     4381 cgatcgtcgg ctccacgcct ctccacgaca tcggcaagat agccgtgccg gactccatca
     4441 tcggcaagcc cggccccctc gactcgggcg agcgcgagcg catgcagcgc catgtcgtcg
     4501 tcggcaagga gatcctcctg gacgcggcga tgcgcgtgag cttcgagccg cgcatcaagg
     4561 tcgccgcgga gatcgcgggc taccaccacg agcgctggga cggcacgggc tatccggagg
     4621 gcaggtcggg cgacgccatc ccgatctccg cgaggatcgt cgccgtggcc gacgtctacg
     4681 acgccatccg ctcggagcgg ccctacaagc ccgcgcacgg acgcgaagcc gcggcctcct
     4741 acgtcaggga caacgcgagc ctccatttcg acccgcgcgt cgtcgccgcc ttcctcgaca
     4801 tcgaggacga gatcgccgag ctctacgcct cctaggcccc gcgccgcccg tcgccgccgc
     4861 ggggcgccgc gtccagggcc gggagccgga ccggcgccga cggccgcgag ggcagactcg
     4921 gaccggggag cccggccgcg acggcctggc aggcgtggct agccctcgag cccgtagagc
     4981 ttgatcttgt tgaggatggt ccgccggctg acgcccagct ccagggcggc tttggtgcgg
     5041 tttccgcccc acttctcgag cgccgccgcc acagcgtccc gctccaggct ctctagggag
     5101 ccgccctggc ggcccagggc gcccgggcgg cccgcgcctc ccccgttccc cgccgccccg
     5161 cccggcccgc cccgcggcgc gccgcgcgcg ccaggctgcg cgggcccgcc ctcggggagg
     5221 ccaagctctc caaggaggat ctcctccccc tcgcagagga tggcggcccg ctccaggacg
     5281 ttctcgagct cgcgcacgtt gccggggaaa cggtaggccg agagggccgc gagggcctcg
     5341 cccgcgaggc gcttggccgg ccggccggac cgggcggcga tcttgcccag gatgtggccc
     5401 gcgaggaggg ggatgtcctc cgcccgctcg cgcaggggag gcacctcgac gcggacgacg
     5461 tcgagccggt agaagaggtc ctcgcggaag cgcccttcgg cgacggcggc cttgaggtcg
     5521 cggttcgtcg cggcgacgag cctggcccgc acggggatgt ccctcgtgcc gcccagccgc
     5581 ctcgtcttcc gctcctggag cgcccgcagg agcttcacct ggagcgagag cggcatctcg
     5641 cccacctcgt ccaggaaaag gatgcccgag cccgcgagct cgaagagacc gggcttccgg
     5701 tcatccgcgc ccgtgaaggc ccccttctcg tagccgaaga gctcgctctc cgcgagctcc
     5761 aggggcaggg ctcccaggtt cacggcgacg aagggctcgt ccctggccgg cgaggcctcg
     5821 tggatcgccc tggctacgac ctccttgccg gtcccgctct cgcccgtgac gaggacggtg
     5881 gcgtccctcg ccgccacctt gcccaccagc cgccgtatgt cgcgcatcgc gcggctctcg
     5941 ccaagcaggc cgccgcctga cggcctggtc cgcgcgcccg cctccacgag gtcctcgcgc
     6001 ctccgcgcgg cgaccaggga cttgagcctc gccacgagct cggccggatc gaagggcttc
     6061 acgaggtagt cgctcgcccc cgtcttgagg gcgtttaccg cgtccccgat ctcccccagg
     6121 gcggagatca tcacggccgg cgtccggagg ccctctgcgc gcatccactc caggaggccc
     6181 tgcccgccca tgcccggcat cttgaggtcc aggaccaccg cgtcgaaggc ctcctccgag
     6241 aggagggcgg ctccggcctc gccgtcgggc gccgtcgccg actcgatgcc ctccagggcc
     6301 aggaggcgcc ccagggattc cctgatgttg cgctcgtcgt cgacgatgag gaccctcatg
     6361 gcgccccgcc tccggggacg ccgtcccccg cggctcggcg ctcgtcgcga aggcgcgtct
     6421 ccccgccgcg cgcctcgccg cccggcggag ccgccgcggc cagcggcagg acgagccgcg
     6481 ccctcgtccc gcctccttgc ctctcctcca ggctgatccc gccgcccgcc gcctcggaga
     6541 agcgcctgca gacggagagg ccgatcccgg tgccccggct cttcgtggtg tagaagggat
     6601 cgaagacccg ctcccggtcc ccgggcgcta tgccggagcc gcggtccagc acgtcgatcc
     6661 gcgcctctcc ctcggccgcg ccgacctcga cggcgagctc ctcctcgggc ccgcccgact
     6721 cgatggcgtt ctcaaggagg ttctcgagga cggagcgcag ccgcgcggga tcggcgagga
     6781 cggcggcctt tccgcgcacg gccacgatgt cgcggcccag gcgcctgagc gagaccgagg
     6841 aggcgacctc cgcggcgtcc acgggaacgg ggcgccccgc cggatcgcgg aggtagtcgc
     6901 tcacgcggcc cgacagggcg gagagcctcg cgacctcgtc gtcgatgatg cggagctctc
     6961 ggctggccga ctcggggcag gtgcgaagga ggatgctcgt ctgcagcttt atcgagagga
     7021 ggggattctt gatctcgtgg gcgatcgtgc tcgcggcggt gccgagcatc acgaggctgc
     7081 gctgctcctc gatccgcttc ctgtactccg cgttgcgcag gacgaggccg cgcacgagga
     7141 ctacgaggca ggcgaggagg agctccgccg cggggaatag gaagcggtgg aagcgttcgg
     7201 cggcccagta ctccccggct tcgacctcca aggcgaggag ttcggcgtcg cgcaacgtcg
     7261 aaaagaagaa ggatggctcc cttccctcgt ccccgtcctt gggctggtcc tcgggccgtc
     7321 cgtcggtccg gccgccgtcg cgccggggcg gcggaggccc cttgggcttc ggatcgagga
     7381 ggatgacgag ggaggagtcc gccggattct cgcggtagat ccggccgtcg ccgtcgagcc
     7441 cggccgaggc agccgcggcg gcggccttag cggcggcggc ggactcctcc gccgaggagg
     7501 cctgtccggc cgccgcgtcg cccggccgcc ccccctcgag ctcggccgcg agctccttgg
     7561 cggacgcgcc gagctcgggc atcttgccga aggaatagag ggtcctccct tccgcgtcga
     7621 aggcggcgac gcccaggacc ttgccgcgca gcgagggcag ggcctcgatg gccgccccga
     7681 agtcgggata gtcccggagg ctcgagagga ggatgttcat ccggcgctcc gcgtcgttcc
     7741 ggctctccag cctgttccgc gcggacatgc cgcggaagat cgcgacgtcg acggcggtgg
     7801 ctcccgcgaa ggcgaggagg gcgaggacga gggagaggtg gcgcagcttc atgggcttca
     7861 tcatccgccg ggattctatc atccccgcaa ggccgcgata aggagctcct cgtcctccag
     7921 gccagcgagc cgtcccaggc cggagaggga ggagatgaag ccgaagcggg ccgcgaccag
     7981 ggccttcctg gccgagcccg cgagggcctc ggcgtcggag agctcggccg acgaggcggc
     8041 ggagagggcg aagagctcgc gcaccgcctc gagccgcgac tcggcgtgct ccagggcctt
     8101 cgccgaggcg acgaggctcc tcgccagggc cgcgcagtcg taggccgcgc tctccacgcc
     8161 gagggccgcc ttgcgcttcg cctcctcgag cccgatcgcc gcctttcccg ccgcgagcct
     8221 cgcggattcc agcccggcct tcttcgtcca gaagtccagg gagagggagg cgctcaccga
     8281 gagggagcca gagcccaggc caaggctttc ccctgccccc aggggaacgg tgtgggagta
     8341 gcccgcggag acgccgggta gccaggccct ctccgcgagc ttcacggaag cggccgcctt
     8401 gccgctcgcg agggccgcgc tcgcggtctc cggatccgat tcggccgcgg cggccgccag
     8461 cctcgaagcg gccgcggaga ccgcctcgtc ggaggcgagg gcgaggccgc gggcggcctg
     8521 gtcgtagcgg gcggcgtcga cgccggccaa ggccgtcccc gaaggaaggc ccgtgagcga
     8581 ggcgagggcc gcgcgagaga gcgagacctc cctcctcgcc ttgacgaagg ccgcctcggc
     8641 cgtcgccgcc tccgactcga cctcgagaag gtcgggccgc gagagggcgg aggcctcgta
     8701 cttcgccgtc gctagcccga ggtgaagaga ggaggactcg aggtcgctct tcgcggcctc
     8761 gagcgccgcc tcggcttcga ggacgccgaa gtaggcggac tcgacctcgg cctcgagaga
     8821 ggagaccgcc gagcggaccg actccctggc catgcccgtg gccgccgcgt cgatggcctt
     8881 cctgagggcg ctccgccctc cgtcgtagag gggctgggcg atcccgacgc ctgcgctcgc
     8941 cgcgacggct ccggaggccg agctcccgta ggcgtacttc ccgcccgcgt ccacagagag
     9001 cgaaggcagg aacgagtagg actcgagccg ctcggccagg acggcctcgt cgaccgcgag
     9061 gagggcctgg cgcacggcgg ccgagcgggc cagggcgagg gacttggcct cctcgaggcc
     9121 gaggggcgcg gacgcggaag ccgcgttttc ggccgcggcg tccgttgaag ccggcgcggc
     9181 tgccagcgcg gcggcggccg cggaggcggc gggctggccc ggggcctcga ggggaagcgc
     9241 cgcggcgccg agcgcggagc cgagcgcgag gccgaagcct agcgcgaggg cggcgagcct
     9301 gttgggtctc atggaaatct ccttattcat agcggagcgc gtcgatggga tagaggttgg
     9361 acgccttccg cgcggggtag tagccgaaga agacgccgac ggcggcggcg aaggcggccg
     9421 aggtcgcgac gacggcggga ttcacgatgg ccggtatccc ggcggagccg aggaccacgc
     9481 agacgaggac ggcgaggaga atgccgacca gccctcctag gaggctcagg atgacggact
     9541 ccgcgaggaa ctggaggagg atgtccctct tccgcgctcc caccgacatg cgtatgccta
     9601 tctccctcgt gcgctcggtc accgagacca gcatgatgtt catgatgccg atgccgccca
     9661 cgaggaggga gacgccggct atcgcggcga ggagggtcgt gagggtcttg gaggtctcgc
     9721 tcgccgtcgc gatgatctcg gcctggttca tcacgtcgaa gtccgcctcg gcgcccggct
     9781 ggatccggtg ggcctcccgg aggatcgact ccacctcggc ctgggcggcg tccatgaact
     9841 cctcgccgac cgcgctcgcc tcgatcgagt tgatgtagcg gtccttgttg agccgcgtga
     9901 gggcggtgtc gagggggacc atcaccacgt cgtcctggtc gtcgcccatc ccgctcgagc
     9961 ccttcttgga caggacgccg acgacggtga agggcgtcgc cccgatcctg acgcgctcgc
    10021 ccaggggatc gtccgtcccg aagagctcct tcgcgacggt ggccccgagg acggcgacct
    10081 tgctccgcga ggccaggtcc gcctcggaga agaaatcccc gctctccacg ccccagccct
    10141 tgatctcgag gtaggagggc tcgaccccct gcacggaggt ctcgtagtag cccgagccgc
    10201 cgacgacctt ggcggagagg cgcacggtgc cggatatcgc ggccaggctg ctcgactcgg
    10261 ccctgagccg cttcacgtcc gccgtggaga gccggttggc ctcgcggggt ccgcggggcg
    10321 gcatcaccat gatgaggttc gtgcccatgg aggagatgcg cgccttgatc tgggcctggg
    10381 agccctcccc caccgcgacc atgacgatca ccgagcagac gccgatgatg atcccgaggg
    10441 aggtgaggat gctccgcatc cggttgcgga agatgctgcg ggcagagagg acggccagct
    10501 tggacgcggt cacgagaccc tccttccgcc cgagaggagc gcgttgcgct ccttccaggc
    10561 ggccaggtcg gcggcggcct cgcgccgcgc ccggaccggc tcgtcggaga cgatggcccc
    10621 gtcgcgcatc gcgatcaccc ggcgcgtgta ggccgcgacc tcgggctcgt gcgtgaccat
    10681 cacgatggtc ttcccgcgcc cgttcaggtc ctggaagagg ctcatgacct ccagcgtcat
    10741 ctccgtgtcc aggttgcccg tcggctcgtc ggccaggatg aaggccggat cgttgacgat
    10801 ggccctcgcg atcgcgaccc gctgctgctg gccgccggag agctgggagg gcttgtggcg
    10861 gatccggccg cccaggccca cctcctccag ggcgcgccgc gcccgctcct cgccgtcgcc
    10921 ccgcctcgcg ccccgggcgc cccccctggc gtagaagagg ggcagctcca cgttctccag
    10981 ggccgtcgtc cgcgacagga ggttgaagcc ctggaagacg aagccgatct tgcggttgcg
    11041 gatctcggcg aactggtcgg cgcccgcctt ggacgcgtcg acgccgtcca ggaggtagat
    11101 gccctccgtc ggcttgtcca ggcagccgag cacgttcatg agggtggact tccccgagcc
    11161 cgaagggccc atcacggcga cgaactcgcc ctgctcgacc ctgaggtcga cgccgcgcag
    11221 ggccttcacg acgacctcgc cgacctcgta gtgccgcttg atccccgaaa gctcgatgac
    11281 cgcgctcatt ccgccctcgc cttgaggacg accttccgtc cctcgagctc cgcgtcggag
    11341 gagacctcgg tgtacgtacc gtcgctcgcg ccggcctcga ccaggatgac gccgaaggcg
    11401 cccgagccgt cgaggtacca gagccgcttg agctcagcgg cggccccgga gaggccgccg
    11461 gcctccgggc ccgcggcgcc ggcggcgaag cccgcggcct ggccggagga gccggccttc
    11521 gccgtcgccc gtccgcgcat gccgggaggg ccgccggggc cgggcccctg catcaccatg
    11581 ccggccagcc caccggcctg cgcctggccg cccgaggccg cggcggcgtc cttggccgcc
    11641 ttggccgcct cgtcgtagcg cgacagggcc tcggcccgct ccgcctcgcc caggcccgag
    11701 agcccggccg cgaagaccag cctcgcgacc tcctcctcgg agaggccggg gggcgtgaac
    11761 ttgagggcgg cgttcggcac gaccaggacc ccctcgcggc tctccttcac gaactcgatg
    11821 tccgcggtca tgccggggaa gagccttccc tccttgttgt cggcggccac catcacgcag
    11881 taggagacga cgttgtccga cgactcgggc accagcctga tctcccgcac gctgcccttg
    11941 aaggaaaggc cggggcaggc ctcgaccgtg aagcgggcct cctgcccgac cttgatggag
    12001 gcgatgtcga gctcgtcgac ctcggccttg atctccatcc gcgacaggtc cttcgcgagg
    12061 gtgaagaggc tcgaggagtt ggaggaggag ccctccacca cgccctggcc gacgtccacg
    12121 ttccggtcca gcacgatgcc gtcgatgggc gagacgacgc gggcgtagct cccgagcttg
    12181 agctcgatca cctccagggc cgactcggcg gaggcgaggg aggcggcgca ggactcgagg
    12241 gtcgccttgc ttgtcttgtt ctcgtagtcg gagacgagcc ccttctcgaa gagcttcgcc
    12301 ttgttccgct cgtccagggc ctggagctcg taggcggcgc gggccttcct gacctcggcc
    12361 agagcctcct tcctctggag ctccaggagg tcggtgttga ggagggtc
//
LOCUS       DAOOSJ010000050        12080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3510_length_12080_cov_6.849064, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000050 DAOOSJ010000000
VERSION     DAOOSJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_12080_cov_6.849064"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLG14_04000"
     CDS             <1..839
                     /locus_tag="PLG14_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOX48326.1"
                     /translation="VGDAVIFRHTLFSLPFALAAVLLETAGKPPLGKLLLIVAAAASG
                     RNLANALNRLIDRDIDARNPRTAGRHLPSGRLESRDLVLFSAAMGAVLVASAAALGWL
                     CLALLPLAGIAVFGYSYTKRFTALCHFWLGATCAIAPMGALVALSGRLFVPRYFVLAG
                     AVALWVAGFDILYALQDIEVDRREGLHSVPARLGAKGARAVAALAHLGTLAGLAALPF
                     LWPLGPAYWAGFAVAAALLAGEHLVALGGTERHIRIAAYGINEVIPLVFLAAVTLDVY
                     MV"
     gene            847..1515
                     /locus_tag="PLG14_04005"
     CDS             847..1515
                     /locus_tag="PLG14_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOX48327.1"
                     /translation="MPRYLVCLTGASGAIYGLRLLKALAATEAELHLVASAWGERVVL
                     EETGRPLSAWLEELGGAAPGPCAAPAGTPGAAPGAAEAHKCRITRHSTDDLAAPVASG
                     SFRLDGTAVVPCSMGTAGALASGLVQNLVHRAGAVALKEGWPLVLAPRESPLSLVDLR
                     NLSALAEAGAAIMPASPGFYHRPATIEELVDGFVARILDRLGAPNPLRRPWQGPATNQ
                     GDLP"
     gene            1512..2441
                     /locus_tag="PLG14_04010"
     CDS             1512..2441
                     /locus_tag="PLG14_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOX48328.1"
                     /translation="MNLVKKAAGLGLLLAAIAASPAAAAPAALKVGVLPDADSLPLLV
                     ADAEGRFAKEGLTVELVPFKTAIERDAALQSGAVDGVVSDLLAAALATQGGFEVRVTS
                     LTDGRYGVVTAPGSGLEKAAQLSGVEVGISSNTIIQYAVDSLLSKAGLPPAEIKGLAV
                     PKIPVRMELLLTGQLKAACLPEPLLTAAQVRGARLVAASDDAGLRAGVLLFSRKAVDS
                     RQAELKAFYRAYASAAAAINADQAKYRPFLVEKAGFPEEVREAYRFVRYERPRLPTEA
                     DVKAVLSWMAAKGLLKADIAPAALLDGRALSGL"
     gene            2449..3300
                     /locus_tag="PLG14_04015"
     CDS             2449..3300
                     /locus_tag="PLG14_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48329.1"
                     /translation="MLEFEGVSIAYRVPDGRLLAVEGASASLPEGAIGALVGPSGCGK
                     TSLVQAAAGLLKPAAGRVLVAGKELRAVRERTAVIFQDFGLLPWKTVEENAELPLLLR
                     RIPRAERRRRVAPVLEELGLLPYRSFFPARLSGGMRQRVAVARALASDPDLLLMDEPF
                     SSLDALTREGLQESLLSAARRRRMTVLIVTHSIEEAAFLSDRAYVMTGRAPGRVSALV
                     EPPAGTAEDSRAAAAGGGQAVGGADQRGPAFRGPAYRGPAYRGSPLYFEYCVAIRRSL
                     EAAEAGA"
     gene            3297..4076
                     /locus_tag="PLG14_04020"
     CDS             3297..4076
                     /locus_tag="PLG14_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48330.1"
                     /translation="MSSARRAMRRLTASKALRSLAAFAVLVAAWAGAAALVAKPFLPA
                     PGLVFERLGARLVDGSLGFHTAVSARRILLALCLAAPAATALGLAAGRSGRLDAFVSP
                     FVYLLHPLPKVAFLPVIMLFFGLGDGAKVFLIGLIIFGQILVAARDAARAVPPALLDS
                     VRSLGAGRLGLLRHVVFPAALPALLTALRVGLGTAIAVLFLAETFATESGLGWYIIDA
                     WSRVDYVDMYAAIAALSAFGFLSFLLVDAAEAFFCRWRRTD"
     gene            complement(4266..5915)
                     /locus_tag="PLG14_04025"
     CDS             complement(4266..5915)
                     /locus_tag="PLG14_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOX48331.1"
                     /translation="MRDLVPLPRSLEELPGALDLSRGGLVAAAPGLEPQGRLAAAWLA
                     EALGRPFQSAVGADAGSARARDGAAGGQAAEPAADRAGFDRAAGAAAPAEPPLVELRL
                     DAALSGPEDYVLTIGPGGAAVAASSPEGAVRGLSTLRQLALSEGPVIRSLKIEDGPRF
                     PWRGLMLDCARSFFRVEFIERLLDLAALHKLNVFHWHLTDDQAWRLDLPGLPELAAAG
                     SRRRDSRIHGVAWREGSYSAADARRVVAYAAERGIMVVPEIETPGHAIALLSSHPELS
                     CAGAAAGGLPFLPEDRFGVFEDILCAGNEEVFALLGKVFDGLAGLFPGPFVHVGGDEA
                     PKARWLLCPKCRSVMKARNLRGPGGELDPERLQSWFMGRVAALLAERGKRMVGWDEIL
                     EGGVAGPGGEGVPRDAVAMSWRGYSGGAEGARAGLEVVMCPQTKACYLDHKHLDLPEE
                     PGNLGVCTVRDSYAFEPLPAGLSPEEAALVKGGQANLWTEMVYFGRQAEYLLFPRLAA
                     LSEALWSPKEARCFEDFARRLPSHGRRLDALGVNRYRGPLF"
     gene            complement(5929..7284)
                     /locus_tag="PLG14_04030"
     CDS             complement(5929..7284)
                     /locus_tag="PLG14_04030"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOX48332.1"
                     /translation="MRKETKLRADGFPADFLWGCSTASYQIEGATAADGRGPSIWDAF
                     CREPGRVLGGQNGDFACDSYHRAAEDVALLKGLGVGAYRFSVAWPRLQPEGRGMPLQA
                     GLDYYSRLVDSLLEAGIEPWASLYHWDLPLALEEAGGWPARDTALRYAEYAEICFRFL
                     GGRVKRWATLNEPWCSAILGYKTGEHAPGRRDARAAYRAVHHLLLGHGLAVRAFRATG
                     LPGEIGIVVNPATPRPATARPADLAAADRAADERTALWLDPVHGRGYPARHLAAQGAD
                     MPVLAGDMEAIAAPIDFVGVNYYNEDAVEAAPGGPEAPEGFRSVPTWQEKTEMGWDIE
                     PRGLYRVLSKIAERWPVKALYVTENGAAFRDEADAAGRVRDLDRVDYYRGHLGACRDA
                     VRDGVPLRGYFAWTLMDNFEWAWGYSRRFGLVSVDQRTGERRPKASYFYYRDVVAGHG
                     L"
     gene            complement(7290..8150)
                     /locus_tag="PLG14_04035"
     CDS             complement(7290..8150)
                     /locus_tag="PLG14_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOX48333.1"
                     /translation="MSEELKTTFAGRLSAAVGRGLAYATMVLFSAMTLYPIFWLAMNS
                     FKTTREFQVSQLGFPKSPTLANYKEAWEMGEFAKLFPNSLVYTIGATAGIIFFALLAG
                     FAFAKLKSRATKAIYGSFVVGILLTTNTLMIPLFLEMNLLGIYNTRLAVLLVYVGAGL
                     PTAIYLSTEYIKSIPTALVESARIDGAGFLRIFMKIILPMTLPVATTLAILNITSIWN
                     EFALINILVSRTELKSLPLGMFKFSGSLSTDYGKQFAALTIGMAPMLLFYVAFRKQIT
                     KGVAAGAVKG"
     gene            complement(8155..9276)
                     /locus_tag="PLG14_04040"
     CDS             complement(8155..9276)
                     /locus_tag="PLG14_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOX48334.1"
                     /translation="MASRKRVDLEESRAYWTMVLPAFLIYLLVMAFPIVLSILLSVSK
                     YSGGKMFGGEPWGFGGLSAYRRIFADPFFWAALKNNLYIVLVSVLGQLPLGFVFAYII
                     YRRSVKWPDFWQGVLYVPNIISIIVVGLLWQVIFSPSGPIAELMNSAYRADFLGKVTG
                     IFDAAGGFNVTDGLVRKVLDAAGPDALGYFTDPVPELKELILSYGPDQLPTVLGDLTN
                     LFARKWSPTFLNKPDVAMLPVLFVILWMYTGMYLILFLANMQKIDSQIIEAARIDGAT
                     EGQVMRLVILPALSGTIVNSAILAVSGSLSSFALVFAMTGGGPSRVTEILSIYMYNNA
                     FLGRPNFPLANAISLVMVMISFLLIALTKALEKRFGGKE"
     gene            complement(9463..10764)
                     /locus_tag="PLG14_04045"
     CDS             complement(9463..10764)
                     /locus_tag="PLG14_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX48335.1"
                     /translation="MKRAILCLMLALAAFGAFAQEKVTLNVFNYQQADQAGYKEDVAI
                     WEKFQKDNPDIVLNMEVLFNDPYHQKLQAYAAAGTLPDVFYVWPGARSAVIHEKKLSK
                     DLAKLLGADYLKDFTAAATNPNNQLGKYMAMLPQSFTYTSVMYVNKKLLADNGFAMPK
                     SYADLKKMVPKLKAKGINVAMLPNKDEWPMQSCLFSTVAGRMAGDQFFNDVLAGKAKF
                     TDKPFVESLKVIENLYKDGILAREDNQGGYGEAPGLFVSGKAAIYIDGDWRTGAYITD
                     KASGKALIDPKAQATDFELLPFVSIPGEKNPGVLSAVAGTGWAISGSLPSGSAKEKAA
                     VRLVKYLYSPEVQAIRYGTGAYVPTRKGVKAELEPLPAKLPPFYAANGKTCYVIDGVL
                     DPAVYNVINAGLKGIGLGTMTSEQVAADVQKAYETWKAATK"
     gene            complement(10817..11971)
                     /locus_tag="PLG14_04050"
     CDS             complement(10817..11971)
                     /locus_tag="PLG14_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOX48336.1"
                     /translation="MIKQSAPGTTTAARIFRTIWRHPRISRVGIAERLGLDKSTVTNQ
                     VSQLIDLGLIEEVEEGASSSKGGRKPIQLGIRKSFGRVIGIELQVESYLAVAVDLSGE
                     ILGVRSGAIRLDSANFADTVERIVAETRKAFGGGTGPLLGVGVGTGGLVDYKRSLIRY
                     SVPLDITEPLDFGRKVASRLPVPCFIENDANCCAWGELAFNRSEELRDFLFALIEFRR
                     DPTSIGSYGGLGVGFGVVLGGKLHPGAHGNAGEFRSAFCDGRGERQFSLSREELSRFA
                     TDREVILKVTDELSRNLAMLVNTMDFDRVYIGGDIEALPVDVPAAFRRRLEENWMYPF
                     PKGVEIRYSSLGEKAVAYGAAGMVLDRLASESLIPGLYPGSAKAPVGGPK"
BASE COUNT         1680 a         4261 c         4321 g         1818 t
ORIGIN      
        1 tggtgggcga cgcggtgatc ttccgccaca ccctcttctc cctccccttc gccctcgccg
       61 cggtccttct cgaaaccgcg gggaagccgc ccctcggcaa gctcctcctc atcgtcgcgg
      121 ccgcagcctc ggggcggaac ctcgcgaacg ccttgaaccg cctcatcgac cgcgacatcg
      181 acgcgcggaa tccgaggacg gccggccgcc accttccctc gggaaggctc gaaagccgcg
      241 acctcgtcct cttctccgcg gcgatgggcg ccgtcctcgt ggcctcggcg gcggccctgg
      301 gctggctctg cctcgccctc cttcccttgg ccgggatcgc agtcttcggc tactcctaca
      361 cgaagcgctt caccgccctc tgccacttct ggctcggcgc gacctgcgcg atagccccga
      421 tgggcgccct cgtcgccctc tcggggaggc tcttcgtccc gcgctacttc gtcctcgcag
      481 gcgcggtggc cctctgggtc gcgggcttcg acatcctcta cgccctccag gacatcgagg
      541 tggacaggcg cgagggcctc cactcggtcc cggcccgcct cggcgcgaag ggggcgcggg
      601 ccgtcgcggc cctcgcccac ctcggaaccc tcgcgggcct ggccgccctc cccttcctct
      661 ggcccctcgg accggcctac tgggccggct tcgccgtcgc ggccgctctc ctcgctggcg
      721 agcacctcgt cgccctgggc ggcaccgagc gccacatacg gatcgcggcc tacgggatca
      781 acgaggtcat ccccctcgtc ttcctggccg ccgtcaccct cgacgtgtat atggtataat
      841 cgggcgatgc cgcgctacct cgtctgcctg accggcgcct ccggcgcgat ctacggcctc
      901 cgcctcctca aggccctggc cgcgacggag gcggagctcc acctcgtggc ctccgcctgg
      961 ggcgagcgcg tcgtcctgga ggagacgggc cgccccctct cggcctggct cgaggagctc
     1021 ggcggcgcgg cgccggggcc ttgcgcggcg ccggccggga cgccaggcgc cgcgcccgga
     1081 gccgccgagg cgcataagtg ccgcataacg cgccactcga ccgacgacct ggccgcgccc
     1141 gtcgcctcgg gctccttcag gctggacggg acggccgtcg tcccctgctc catgggcacg
     1201 gccggcgccc tggcctccgg cctcgtccag aacctcgtcc accgcgccgg ggccgtcgcc
     1261 ctcaaggagg gctggcccct cgtcctcgcg ccgcgcgagt cgcccctctc gctcgtcgac
     1321 ctgcggaacc tctcggccct cgccgaggcc ggcgcggcga tcatgcccgc ctcccccggc
     1381 ttctaccacc gcccggccac catcgaggag ctcgtcgacg gcttcgtcgc gaggatcctg
     1441 gaccgcctgg gcgcgcccaa ccccttgcgg aggccctggc agggccccgc gaccaaccaa
     1501 ggagatctcc catgaacctc gtcaagaagg cggccggcct cggcctcctc ctcgcggcca
     1561 tcgccgcctc ccccgccgcc gccgcgcccg cggccctcaa ggtcggcgtc ctccccgacg
     1621 ccgactccct ccccctcctc gtcgccgacg cggagggccg cttcgccaag gagggcctga
     1681 cggtggagct cgtgcccttc aagacggcga tcgagcgcga cgcggccctg cagtctggcg
     1741 cggtcgacgg tgtcgtctcc gacctcctgg ccgcggccct cgcgacccag ggcggcttcg
     1801 aggtccgcgt cacgagcctc acggacggcc gctacggcgt cgtgaccgcc cccggctcgg
     1861 gactcgagaa ggccgcccag ctctcgggcg tcgaggtcgg catctcatcc aacaccatca
     1921 tccagtacgc ggtcgactcc ctcctctcca aggcgggact gcccccggcg gagatcaagg
     1981 gcctcgccgt gcccaagatc ccggtgcgca tggagctcct cctcacgggc cagctcaagg
     2041 ccgcctgcct ccccgagccc ctcctcaccg cggcccaggt tcgcggcgcg aggctcgtcg
     2101 cggccagcga cgacgcgggc ctccgcgccg gcgtcctcct cttctcccgg aaggccgtcg
     2161 actcccgcca ggccgagctc aaggccttct accgcgccta cgcctcggcc gccgcggcca
     2221 tcaacgccga ccaggcgaag taccgcccct tcctcgtgga gaaggcgggc ttccccgagg
     2281 aggtccgcga ggcctaccgc ttcgtgcgct acgagaggcc ccgcctcccc acggaggccg
     2341 acgtgaaggc cgtcctgtcc tggatggccg cgaagggcct cctcaaggcc gacatcgccc
     2401 ccgccgccct cctcgacggc cgcgccctct ccggcctcta gcgggtccat gctcgaattc
     2461 gaaggggtct cgatagccta tcgagtcccc gacggccgcc tcctggccgt cgagggagcc
     2521 tccgcgagcc tccccgaagg cgcgataggc gccctcgtcg gaccctcggg ctgcgggaag
     2581 acgagcctcg tgcaggcggc ggccggcctc ctaaagcccg ccgccggccg cgtcctcgtc
     2641 gcggggaagg agctccgcgc cgtccgggag cgcacggccg tgatcttcca ggacttcggc
     2701 ctcctcccct ggaagaccgt ggaggagaac gcggagctcc ccctcctcct ccgcaggatc
     2761 ccgcgggccg agcgcaggag gcgcgtcgcg cccgtcctcg aggagctcgg cctccttccc
     2821 taccgctcct tcttccccgc gaggctctcc ggcggcatgc gccagcgcgt cgccgtcgcg
     2881 cgcgccctcg cctccgaccc cgacctcctc ctcatggacg agcccttctc cagcctggac
     2941 gcgctgaccc gcgaaggtct ccaggagagc ctcctttcgg ctgccaggcg gagacgcatg
     3001 acggtcctca tcgtcacgca ctcgatagag gaggcggcct tcctctccga ccgggcctac
     3061 gtgatgacgg gccgcgcgcc cggccgcgtg agcgccctcg tcgagccgcc tgccgggaca
     3121 gcggaagact cccgcgccgc cgcggccggc ggcggccagg ccgtcggcgg cgctgaccag
     3181 cgcggtcccg ccttccgcgg tcccgcgtat cgcgggcccg catatcgcgg gagtcccctc
     3241 tatttcgagt actgcgtcgc gatcaggcgc agcctggaag cggcggaggc gggggcatga
     3301 gcagcgcgcg acgggccatg cgccgcctca ccgcctccaa ggccctgcgc tccctcgcgg
     3361 ccttcgccgt cctcgtcgcg gcctgggcgg gggcggccgc cctcgtcgcg aagcccttcc
     3421 tcccggcgcc cggcctcgtc ttcgaaaggc tcggcgcgag gctcgtcgac ggctccctcg
     3481 gcttccacac ggcggtctcg gcccgccgca tcctcctcgc cttatgtctc gccgcgcccg
     3541 ccgccacggc cctgggcctc gccgccggcc gctcggggcg cctggacgcc ttcgtctcgc
     3601 ccttcgtcta cctcctccac cccctgccca aggtggcctt cctcccggtc atcatgctct
     3661 tcttcggcct gggcgacgga gccaaggtct tcctcatcgg cctcatcatc ttcgggcaga
     3721 tcctcgtcgc cgcccgcgac gccgcgcggg ccgtgccgcc ggccctcctc gactcggtgc
     3781 gttccctggg cgcgggccgc ctgggcctcc tccgccacgt cgtcttcccg gccgccctgc
     3841 ccgccctcct caccgccctg cgcgtgggcc tggggacggc catcgccgtc ctcttcctgg
     3901 cggagacctt cgcgacggag tcgggcttgg gttggtacat catcgacgcc tggtccaggg
     3961 tcgactacgt ggacatgtac gccgcgatag ccgccctctc ggccttcggc ttcctctcct
     4021 tcctcctcgt cgacgcggcg gaggccttct tctgccgctg gaggaggacg gactaggccg
     4081 aggggccggc tgggcccgcc gcgcggcggc ccagggcggc gcgccgaggc ccggggccct
     4141 cgcggcggca ggtgcggctt cgcgcccggg cccggggctt tgcggcctcc gacgcgggcc
     4201 cgcgcgcggc gtccgtcggc gaccgaccct cgtcggcccg agcgaaccga ggcgagctcg
     4261 gctggctaga agagcgggcc gcggtagcgg ttgacgccca gggcgtccag gcggcggccg
     4321 tgtgagggca ggcgccgcgc gaagtcctcg aagcagcgcg cctctttcgg gctccagagc
     4381 gcctctgaca gggccgcaag ccgcgggaag agaaggtact cggcctggcg gccgaagtag
     4441 accatctcgg tccagaggtt ggcctggcct cccttgacca gggccgcctc ctcgggcgac
     4501 aggcccgcgg gcaggggctc gaaggcgtag ctgtcgcgca ccgtgcagac gccgaggttg
     4561 cccggctcct ccggcaggtc caggtgcttg tggtcgaggt agcaggcctt ggtctgcgga
     4621 cacatcacga cctccaggcc ggcccgcgcg ccctccgcgc cgccagaata gccgcgccag
     4681 gacatggcga cggcgtcccg agggacgccc tccccgccgg ggccggccac gccgccctcc
     4741 aggatctcgt cccagccgac catccgcttg cctcgctccg ccaggagggc ggcgacgcgg
     4801 cccatgaacc aggactggag cctctcggga tcgagctcgc cgcccggacc ccgcaggttg
     4861 cgcgccttca tcacgctccg gcacttgggg cagaggagcc acctggcctt gggcgcctcg
     4921 tcccccccga cgtgcacgaa gggccccggg aagagcccgg ccaggccatc gaagaccttg
     4981 ccgaggagcg cgaagacctc ctcgttcccc gcgcagagga tgtcctcgaa gacgccgaag
     5041 cggtcctcgg gcaggaaggg caggcctccc gccgcggcgc ccgcgcagga gagctcgggg
     5101 tgggaggaaa ggagggcgat cgcgtggccg ggcgtctcga tctcggggac caccatgatg
     5161 ccgcgctcgg cggcgtaggc gacgacgcgc ctcgcgtcgg cggccgagta ggagccctcg
     5221 cgccaggcga cgccgtggat gcgcgagtcc cgccgccggg agcccgccgc ggcgagctcg
     5281 ggcaggccgg gcaggtccag gcgccaagcc tggtcgtccg ttaggtgcca gtggaagacg
     5341 ttgagcttgt gcagggccgc gaggtccagg agccgctcga tgaactcgac gcggaagaag
     5401 ctccgcgcgc agtcgagcat gaggccccgc caggggaagc gcgggccgtc ctcgatcttg
     5461 agggagcgga tgaccggccc ctcggagagg gcgagctggc gcagggtgga aaggccgcgg
     5521 acggcgcctt ccggcgacga ggccgcgacc gcggcgcccc cgggcccgat cgtgaggacg
     5581 tagtcctcag gcccggacag ggccgcgtcg agccggagct ccactaaggg cggctcggcc
     5641 ggagcggcgg ccccggccgc gcgatcgaag cccgcgcggt cggcggccgg ctcggcggcc
     5701 tgcccccccg cggccccgtc ccgcgcgcgg gcgcttcccg cgtcggcgcc gaccgccgac
     5761 tggaagggcc ggccaagggc ctcggcgagc caggccgccg cgaggcggcc ctggggctcc
     5821 aggcccggcg ccgccgcgac caggccgccc ctcgaaaggt ccagggcgcc gggcagctcc
     5881 tcgagggacc gcggaagggg aacgaggtcg cgcatcgcgc ccatcggact agagcccgtg
     5941 cccggcgacg acgtcgcggt agtagaagta actcgctttc ggcctccgct cgcccgtccg
     6001 ctggtccacg ctcacgaggc cgaagcgtcg cgagtagccc caggcccact cgaagttgtc
     6061 catgagagtc caggcgaagt agccgcgcag ggggacgccg tcccggaccg cgtcgcggca
     6121 ggccccgagg tggccgcggt agtagtcgac gcggtccagg tcccgcaccc tgcccgccgc
     6181 gtcggcctcg tcgcggaagg cggcgccgtt ctcggtgacg tagagggcct tcacgggcca
     6241 gcgctccgcg atcttggaaa ggacgcggta gaggccccta ggctcgatgt cccagcccat
     6301 ctcggtcttc tcctgccagg tcgggacgga gcggaaaccc tcgggagcct cggggccgcc
     6361 cggcgccgcc tccacggcgt cctcgttgta gtagttgacg ccgacgaagt cgatcggcgc
     6421 cgcgatggcc tccatgtcgc cggcgaggac gggcatgtcc gcgccctggg ccgcgaggtg
     6481 gcgggcggga tagccccggc cgtggaccgg gtcgagccag agggcggtgc gctcgtccgc
     6541 ggcgcggtcc gccgccgcga ggtccgcggg ccgggccgtg gccggccgcg gagtggccgg
     6601 gttgacgacg atgccgatct cgccggggag gcccgtggcg cggaaggcgc ggacggcgag
     6661 gccgtggccg aggaggaggt ggtggacggc gcggtaggcc gccctcgcgt cgcggcgccc
     6721 gggcgcgtgc tcgcccgtct tgtagcccag gatggccgag caccaaggct cgttgagcgt
     6781 cgcccaacgc ttcacccgac cgcccaggaa gcggaagcag atctccgcgt actccgcgta
     6841 gcgcagggcc gtgtcgcgcg cgggccagcc gccggcctcc tccagggcca ggggaaggtc
     6901 ccagtggtag aggctcgccc agggctcgat gcccgcctca aggagggagt cgacgaggcg
     6961 cgagtagtag tcgaggccgg cctggagggg catgcccctg ccctccggct ggagccgcgg
     7021 ccaggcgacg gagaagcggt aggccccgac cccgagcccc ttgaggaggg cgacgtcctc
     7081 cgccgcccgg tggtaggagt cgcaggcgaa gtcgccgttc tgcccgccca ggacgcggcc
     7141 gggctcgcgg cagaaggcgt cccagatgga ggggccgcgc ccgtcggcgg ctgtcgcccc
     7201 ctcgatctgg tagctcgcgg tggagcagcc ccagaggaaa tccgcgggga atccgtccgc
     7261 gcggagcttc gtttctttgc gcatggcgac tatcctttga cggcgccggc cgcgacgccc
     7321 ttcgtgatct gcttgcggaa cgcgacgtag aagaggagca tcggcgccat gccgatcgtg
     7381 agcgccgcga actgcttgcc gtagtcggtg gacagggagc ctgagaactt gaacatgccg
     7441 agcggcaggg acttgagctc cgtcctcgac accaggatgt tgatgagggc gaactcgttc
     7501 cagatgctcg tgatgttgag tatggcgagg gtcgtcgcga ccggcaaggt catcgggagg
     7561 atgatcttca tgaagatcct gaggaagccc gccccgtcga tgcgcgccga ctcgaccagg
     7621 gccgtcggga tcgacttgat gtactccgtg gagaggtaga tcgccgtggg gagccccgcg
     7681 ccgacgtaga cgaggaggac ggccaggcgc gtgttgtaga tcccgaggag gttcatctcc
     7741 aggaagagcg ggatcatgag ggtgttcgtc gtgaggagga tgccgaccac gaagctgccg
     7801 tagatggcct tcgtggcgcg ggacttgagc ttcgcgaagg cgaagccggc gagcagcgcg
     7861 aagaagatga tgcccgccgt ggcgcctatc gtgtagacga gggagttcgg gaagagcttc
     7921 gcgaactcgc ccatctccca ggcttccttg tagttcgcca gggtcgggct cttggggaag
     7981 ccgagctggc tcacctggaa ctcccgcgtc gtcttgaagg agttcatcgc gagccagaag
     8041 atcgggtaca gggtcatggc ggagaagagg accatggtcg cgtaggcgag gccccgcccg
     8101 acggcggcgg agagcctgcc agcgaatgtc gtcttgagct cttcggacat ggcgctactc
     8161 cttcccgccg aagcgcttct cgagggcctt cgtgagggcg atgaggagga agctgatcat
     8221 caccatgacg agggagatgg cgttcgcgag ggggaaattc ggacgcccca ggaaggcgtt
     8281 gttgtacatg tatatcgaga ggatctccgt gacccgggag ggcccgccgc ccgtcatcgc
     8341 gaagacgagg gcgaagctgg agagggagcc ggagaccgcg aggatggccg agttgacgat
     8401 ggtgcctgac agggcgggca ggatgacgag ccgcatgacc tggccctcgg tcgccccgtc
     8461 gatgcgggcg gcctcgatga tctgcgagtc gatcttctgc atgttcgcca ggaagaggat
     8521 gaggtacatg cccgtgtaca tccagaggat gacgaagagg acggggagca tcgcgacgtc
     8581 gggcttgttg aggaaggtcg ggctccactt gcgcgcgaag aggttcgtga ggtcgcccag
     8641 gaccgtcggg agctggtcgg gcccgtagga gaggatgagc tccttgagct cggggaccgg
     8701 gtccgtgaag tagccgaggg cgtcaggccc cgcggcgtcc aggaccttcc gcacgagccc
     8761 gtccgtcacg ttgaagccgc ccgcggcgtc gaagatgccc gtcaccttgc ccaggaagtc
     8821 ggcgcggtag gccgagttca tgagctcggc tatcgggccg ctcggcgaga agatgacctg
     8881 ccagaggagg cccacgacga tgatggagat gatgttgggg acgtagagga cgccctgcca
     8941 gaagtcgggc cacttcaccg agcgccggta gatgatgtag gcgaagacga agcccagggg
     9001 gagctggccc aggacggaca cgaggacgat gtagaggttg ttcttcaggg ccgcccagaa
     9061 gaaggggtcg gcgaagatgc gcctgtaggc ggagaggccg ccgaagcccc agggctcccc
     9121 gccgaacatc ttgccgcccg aatacttcga gacggagagg aggatcgaga ggacgatggg
     9181 gaaggccatc acgaggagat agatgaggaa ggccggaagg accatcgtcc agtaggcgcg
     9241 gctctcctcc aggtcgaccc ttttcctgct tgccatggat acctcgaaag tggaaatggt
     9301 ggagcggggc tcttgcgtgg gggtcgcggc gcggagcctt gcgcccgtcg cggccggggc
     9361 cgcggcccat gcgcccaggc ggccaggccc cggccgggcc gcctgggcgc ggaactcggg
     9421 gcgcggccgc ccttcaaggc gggcggccgc gcctcgatcc gcttacttgg tcgcggcctt
     9481 ccaggtctcg taggccttct ggacgtcggc ggcgacctgc tcggaggtca tggtgccgag
     9541 gccgatgccc ttgaggccgg cgttgatcac gttgtagacg gccgggtcga ggacgccgtc
     9601 gatgacgtag caggtcttgc cgttcgcggc gtagaagggg gggagcttgg cggggagcgg
     9661 ctcgagctcg gccttgacgc ccttcctggt cgggacgtag gcgcccgtgc cgtagcggat
     9721 ggcctggacc tcgggggagt agaggtactt caccaggcgg acggcggcct tctccttcgc
     9781 ggagccggag gggagggagc cggagatcgc ccagccggtg ccggcgacgg cggagagcac
     9841 gccggggttc ttctcgccgg ggatggagac gaagggcagg agctcgaagt cggtcgcctg
     9901 ggccttcggg tcgatgaggg ccttgccgga ggccttgtcg gtgatgtagg cgccggtgcg
     9961 ccagtcgccg tcgatgtaga tcgcggcctt gccggagacg aagaggccgg gggcctcgcc
    10021 gtagccgccc tggttgtcct cgcgggccag gatgccgtcc ttgtagaggt tctcgatcac
    10081 cttcagggac tcgacgaagg gcttgtcggt gaacttggcc ttgccggcca ggacgtcgtt
    10141 gaagaactgg tcgccggcca tgcggcccgc gacggtggag aagaggcagg actgcatcgg
    10201 ccactcgtcc ttgttgggga gcatggcgac gttgatgccc ttggccttga gcttgggaac
    10261 catcttcttg aggtcggcgt agctcttggg catggcgaag ccgttgtcgg ccagaagctt
    10321 cttgttgacg tacatcacgg aggtgtaggt gaaggactgg ggcagcatgg ccatgtactt
    10381 gccgagctgg ttgttcgggt tcgtggcggc ggcggtgaag tccttgaggt agtcggcccc
    10441 gaggagcttc gcgaggtcct tggagagctt cttctcgtgg atgacggcgg agcgggcgcc
    10501 cggccagacg tagaagacgt cgggcagcgt gccggcggcg gcgtaggcct ggagcttctg
    10561 gtggtagggg tcgttgaaga ggacttccat gttgaggacg atgtcgggat tgtccttctg
    10621 gaacttctcc cagatcgcca cgtcctcctt gtagccggcc tggtcggcct gctggtagtt
    10681 gaagacgttg agcgtcacct tctcctgggc gaacgctccg aaggcggcga gcgccagcat
    10741 gagacacagg atcgcgcgtt tcatcgggac ctccttggaa gagcgggcgc cttcgcgccc
    10801 gcgcgggaga cgggaactat ttcgggcccc cgacgggggc cttggccgaa ccgggataca
    10861 ggccggggat gaggctctcc gaggccaggc ggtccaggac catgcccgcg gcgccgtagg
    10921 cgaccgcctt ctcgcccagg ctcgagtagc ggatctccac gcccttgggg aaggggtaca
    10981 tccagttctc ctcgagccgc cgcctgaagg ccgcgggaac gtccacgggc agggcctcga
    11041 tgtcgccgcc gatgtagacg cggtcgaagt ccatcgtgtt gacgagcatg gccaggttgc
    11101 gcgacagctc gtcggtcacc ttgaggatga cctcgcggtc cgtcgcgaag cggctgagct
    11161 cctcgcggga gagggagaac tgccgctcgc cccgcccgtc gcagaaggcg ctccggaact
    11221 cgcccgcgtt gccgtgggcg cccggatgga gcttcccgcc caggacgacg ccgaagccga
    11281 cgcccaggcc gccgtagctc ccgatggagg tcgggtcgcg gcggaattcg atgagggcga
    11341 agaggaagtc gcggagctcc tcgctccggt tgaaggcgag ctcgccccag gcgcagcagt
    11401 tggcgtcgtt ctcgatgaag caggggacgg ggaggcggct cgcgaccttc ctgccgaagt
    11461 ccaggggctc ggtgatgtcg aggggcacgg agtacctgat gaggctccgc ttgtagtcga
    11521 cgaggccgcc cgtgcccacg ccgacgccca ggagggggcc cgtcccgccg ccgaaggcct
    11581 ttctcgtctc ggccacgatg cgctccaccg tgtcggcgaa gttggccgag tcgaggcgga
    11641 tggccccgct cctcaccccg aggatctcgc cggagagatc gacggcgacc gcgaggtagg
    11701 actcgacctg gagctctatg ccgatgacgc ggccgaagga cttcctgatg ccgagctgga
    11761 tgggcttccg cccccccttg ctcgacgagg ctccctcctc gacctcctcg atgaggccga
    11821 ggtcgatgag ctggctcacc tggttcgtga cggtggactt gtcgaggccg aggcgctcgg
    11881 cgatgccgac gcggctgatg cgcggatggc gccagatcgt gcggaagatc ctggccgctg
    11941 tcgtcgtgcc gggcgcgctc tgcttgatca cgatccaaaa gccccttgga aacgatgctt
    12001 ggttggttga tttaagcaac caagcatggt ataccatggg tcttgggagc cgtcaactgg
    12061 cttttttcgg ccttcgagcc
//
LOCUS       DAOOSJ010000051        11969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3557_length_11969_cov_5.539365, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000051 DAOOSJ010000000
VERSION     DAOOSJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_11969_cov_5.539365"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PLG14_04055"
     CDS             complement(<1..951)
                     /locus_tag="PLG14_04055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOX48337.1"
                     /translation="MASHHPAFELLSDREIPEFRARGLLFRHKATGCEVYRLVSEDEE
                     NSFAFAFRTPPPDSSGVAHIVEHSVLCGSERYPVKDAFLVMARRSLATFLNAWTYPDK
                     TVYPAASAAPADYFNLMSVYGDAVFFPRLSEDTFLQEAHHLELSEEGSLDVKGVVYNE
                     MRGDYSSAESLAATAAATSLFTPGHPYSFDSGGDPEAIPSLSYEAFKEFWAACYHPSN
                     CRIFLHGNIDTERQLAFLEERFLGRAEPGKRFAARKPSPGIPFEPPFAAPRRLEVPCP
                     ASEGSEGATTIIVNWLSRPAADAAEALALEVLSEILLGHDG"
     gene            1037..1558
                     /locus_tag="PLG14_04060"
     CDS             1037..1558
                     /locus_tag="PLG14_04060"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOX48338.1"
                     /translation="MSIVTKTGDEGLTSLWSGERVWKDDLRVEAYGTVDELSSFLGLA
                     RHSAALPETLAAVEEVQRDLVRVAGELASRGTPFSRPLLQADEERLTKATEALEARIP
                     LKGFVLPGMTPGSAALDAARTVARRAERRVVALAKEVEVGEPLRRYLNRLSDYLFMLA
                     RAEEAAAGALRYA"
     gene            1555..3210
                     /locus_tag="PLG14_04065"
     CDS             1555..3210
                     /locus_tag="PLG14_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX48339.1"
                     /translation="MIKLSNLRVAYGGRLIFRDEEFLLRPGDKIGLVGPNGAGKTTLF
                     RVLAGLEKPDGGTVSMDPGTTIGYFSQDVGEMSGRSVLEEVLAGAGRVREIGTELEAL
                     ERRMADPEDRGLTDSEMDRYGELQHEFAHRGGYDLEPRAEAILSGLGFERARFAEPVE
                     NFSGGWKMRIALARILLLEPSVLLMDEPTNHLDLESIVWLEAWLKAFQGDLVMTSHDR
                     EFMTRICGRTVEVAQGAVTTYSGDYDFYLREREIRREQLVASQKRQEAMLAKEEEFIA
                     RFAARASHAAQVQSRVKTIEKIERIVVPADPKAIKVRFEDCPRSGDIVVQMEGLAKSW
                     PAPGGGSRAVFEGATGVVRRRDKVALVGVNGAGKSTLLKVVAGLAEPSAGTCSLGPSV
                     RLGYFSQYSSDVLVPSRTIFEEVKERLPRASDGQVKNILGAFQFTGDDTEKRVSVLSG
                     GERSRVMLALILAEPVNFLVLDEPTNHLDIASREVLLDALCEFDGSVMIVSHDRYFLR
                     RLATRVFEIDRGSLSAYEGDYGYYLEKSARVRAEGALVAAGAG"
     gene            3380..5008
                     /locus_tag="PLG14_04070"
     CDS             3380..5008
                     /locus_tag="PLG14_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX48340.1"
                     /translation="MRLSLACLALAFSLAAPPLASAAPAASAAAPAGERILVGYYDAR
                     PSCWRGEDGEAKGIFVDILKEVAVREGWSLEFSYQGWDGLLEALKSGSVDLVPAIARS
                     EPRSSYAAFTEETIFLDWGAVFVGRSGKIRSILDLGGRRVGALENDIWFSGPGALRDL
                     CASFLVEPDYVFFPDYSSLFRALGSGAIDAAAGSNSLGVYWESMEPIAATPIVYLPLE
                     LRVAGPARGSRGSRLVGAVDRRVRELKEETPGALRSVLERYAVPARREYVTPPWVVPS
                     LLLLAAFLALIVLLLLLQAAALRRSHAEAAAALAGLEEAHGSLESSFKEKDLLLHELS
                     HRVKNNFQLILSLISMAQAGEEEERGGSLAEIREKIYALSVAEELLHGGGGVDEAAVR
                     AFVERLASRLSELYGASREDLEISVRLGRPYIDPSAATPLAQALSELAANACRHGRGE
                     DGRASLSIAIESAPDGSGSIVVSDRGPGLPPGFSLARDARLGLGLVSALAAQLRGRLE
                     AGPGPGGRGASFAIRLPAEYWLAPEARPAPGAKP"
     gene            complement(5197..8364)
                     /gene="dnaE"
                     /locus_tag="PLG14_04075"
     CDS             complement(5197..8364)
                     /gene="dnaE"
                     /locus_tag="PLG14_04075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX48341.1"
                     /translation="MRVPLLVSSAYSLGFGAVLPAALARRARELGYGALALSDRNGVY
                     GLPAFVEAAEAEGRRPIMGTELSWPGGRALLLAKDREGFARISRLLTARAEGRLDARR
                     ELADDTRGLVAATDDEALLSLAAGGLADRCASGGASVGALGGAAAGSESASGGASGGR
                     LHALLSPCNRPRWRALLRTGARPLATGEVRFLEAGDRELQRLLLAIAGGKTVAEVGEA
                     ELSDPGALLLSPAAFEAAYAEVPEALAANEEAVESLGLSSLFGGWIFPRRQGCALADG
                     AGAVERGGGGAGRGSGRAGGGADDSSDAGGGAGRGASEAGASGGAPEECGGAAALLRR
                     LVLEGAERRYGKAGPEVLARIDYELGIIEEKGFSDYFLVVRDIAGRASRTCGRGSAAA
                     SVVSYALGITDVDPIRHGLYFERFLNPGRLDPPDIDVDFAWDERDALLAEVLADFGED
                     RAARVANHVRFKERSALREAARAYGMPEGEISGFERRAAADGEAAALEGACDDWRAIA
                     RLAPRLVGLPRHLGVHSGGLVVAPGPLSAIVPLERTGSGTRVTAWDKEGVEAAGLVKI
                     DLLGNRSLAVVRDALANLAENGEGIDEDAWKPLEDEATIEMLGRGDTMGVFYVESPAM
                     RLLQKKTGRGDFEHLVIHSSMIRPAANRYIDEYIDRLGGKPYAPIHPLLDGVLAETFG
                     IMCYQEDVCKAAIALAGFGAAEADGMRKVLSKKDAKERLASFRARFEAGARERGVDEG
                     TVAEVWAMIESFSGYSFVKAHSASYAMLSFKSAFLRRHHPAEFMAAVISNRGGYYSTL
                     AYASEARRMGLGLLGPDANESGLRCRGRGSGLRFGLGMVAGLGQAAASRLVEERKARG
                     PYRSVEDLARRARPDRSDAEALAGSGALDSLSPGLSRADKLMRLLSAAATREEGEGPG
                     LFPDEGPALPRRASKETERRRLEAEMRYLGTTLAVHPLALWPEALSRRRSLAKDLSSL
                     VGRRIELVGWPIAGKTVLAAGEEPMEFVSFEDETALYETVLFPEAYRGYRRLLLEERP
                     LMVRGKVEEDRGGITLAVSSLERIG"
     gene            complement(8374..9546)
                     /locus_tag="PLG14_04080"
     CDS             complement(8374..9546)
                     /locus_tag="PLG14_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOX48342.1"
                     /translation="MASGSILHVNAVGLLAAIEEGLDGSLKGRPFVVANEGAPRAIVL
                     DLSPEAHREGLRRGMALKEARLLCPGLAARPPRPELRDLADERLARAALEFSPLVERA
                     GAGHLFVDLAGTGRLFGPPEDAAQRLRARMAEETGLRPCLALASNKTVSKVATRVFRP
                     GGFVALSPGEESLLLGRQPVGLLPGVGPVLRSRLLLLGIDEIGELAGLSRPEALALGP
                     RGPELAARARGLDASPVDPEPPGRRSLRGEALLEPDTSDPEVLRLRLGALAAGLGFSL
                     RAGGSGAARATVSLVYADGERGSGSARAPGLRARDDELLDLALAALARARKRRVRVRR
                     LCLELSEFVPAGPELDLFEPEDSRLSRLQPALDRIRGKYGAKALSPGALLALGAGA"
     gene            complement(9562..10374)
                     /locus_tag="PLG14_04085"
     CDS             complement(9562..10374)
                     /locus_tag="PLG14_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOX48343.1"
                     /translation="MSSLPPPACALRIGTSGYDYPEWEGVLYPKGLGRKEYLGAYSEV
                     FDAVELNFSYYGMPKAEQIRGMLGRTRRPIDFSIKANQALTHKVDPATWKDDALEFAR
                     GAAPLLEAGRLAAVLLEFPFSFHYKEEERRYLGRLLGELSSFPLAVEFRNAEWYGARV
                     IEGLKERRVALCSVDLPRLEGLPPLSDLVTAELAYVRFHGRNAAAWWTGDSGSRYEYR
                     YSPEELGAWIPRLEAMSAEASKLLVFFNNHRRGDAADNARALSRLAKAARLA"
     gene            complement(10456..11739)
                     /locus_tag="PLG14_04090"
     CDS             complement(10456..11739)
                     /locus_tag="PLG14_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOX48344.1"
                     /translation="MNMKDRIERAYKGKPSPDREAEEAPRLPGEGRRLPEAAPRGPAA
                     ARPAPRREAAPREAAPREAADAARRPTAEPRRPEARTPKALAKAEGQAERLLKTGDER
                     GISKAAKFLLLLGSEEASKVLAHLGAKEIEELSREIIQVKSIDAIEANEILAEFGWLV
                     KTKGYAIEGGPETAEKMLTAAFGPDRARDLMRKAAPETLRPFRFLNDFEPRELQLILK
                     EESPQVLAVILPYLEPRRASGLLERLPEELRIELVKRIARLEKVSPEIIRRVEEGLKD
                     RIRKIGTVSVEEIDGRAALAGILRHVDPRLEGRVLDALDDESPELSKDVRERLFTLDD
                     VLRVPGKYLQKALRDFQDRDLALLLKGRGEDFKAKILGSVSQGRRALVLDEYAVLGAV
                     RREDAAEAAREFLAYLKRAWEDGDLVLEGEDELVE"
BASE COUNT         1618 a         4271 c         4447 g         1633 t
ORIGIN      
        1 accgtcgtgg ccaaggagaa tctcggagag gacctccagg gcgagggcct cggccgcgtc
       61 ggcggcgggc cgggagagcc agttcacgat gatcgtcgtc gcgccctcgg agccctccga
      121 tgctgggcag gggacctcca ggcggcgggg agcggcgaag gggggctcga aggggatccc
      181 gggcgagggc ttccgggcgg cgaagcgctt gccgggctcg gcccgcccca ggaagcgctc
      241 ctccaggaag gcgagctggc gctccgtgtc gatgttgccg tggaggaaga tcctgcagtt
      301 ggaggggtgg tagcaggccg cccagaactc cttgaaggcc tcgtagctca aggaagggat
      361 ggcctcgggg tcgccgccgg agtcgaagga gtaggggtgg ccgggggtga agaggctggt
      421 cgcggcggcc gtcgccgcga gggactcggc cgaggagtag tcgccgcgca tctcgttgta
      481 gaccacgccc ttcacgtcca ggctcccctc ttccgagagc tcgaggtggt gggcctcctg
      541 gaggaaggtg tcctcggaga ggcgggggaa gaagacggcg tcgccgtaga cggacatcag
      601 gttgaagtag tcggccggcg ccgcgctggc ggcggggtag acggtcttgt cggggtaggt
      661 ccaggcgttg aggaaggtgg ccaggctccg cctggccatc acgaggaagg cgtccttcac
      721 cgggtagcgc tccgagccgc agaggacgga gtgctcgacg atgtgggcca ctccgctcga
      781 gtcggggggc ggcgtgcgga aggcgaaggc gaagctgttc tcctcgtcct ccgagacgag
      841 gcggtagacc tcgcagcccg tcgccttgtg gcggaagagg aggccgcggg cgcggaactc
      901 ggggatctcg cggtcggaaa ggagctcgaa ggcggggtgg tgggaggcca tggatagaag
      961 atggcgaggc cggaggcgag cgtcaacagg gggccgggcc ttcccgctcc gcctcccggc
     1021 gggctatatt ccccccatga gcatcgtgac gaagacggga gacgagggcc tgacgagcct
     1081 ctggtcggga gagcgggtct ggaaggacga cctcagggtc gaggcctacg gcacggtgga
     1141 cgagctgtcc tccttcctcg gccttgcgcg gcactcggcc gccctgcctg agaccctggc
     1201 cgccgtcgag gaggtccagc gcgacctcgt gcgcgtcgcc ggggagctcg cctcccgcgg
     1261 gacgcccttc tcccgccccc tcctccaggc ggacgaggag aggctcacga aggcgacgga
     1321 ggccctggag gcgcgcatcc ccctcaaggg cttcgtcctc cccgggatga cccccggctc
     1381 ggcggccctc gacgccgcca ggacggtcgc gcgccgcgcc gagcgccgcg tcgtcgccct
     1441 cgcgaaggag gtcgaggtgg gcgagcccct ccgccgctac ctgaaccgcc tctcggacta
     1501 cctcttcatg ctcgcgaggg cggaggaagc cgccgcgggc gccctccgct acgcatgatc
     1561 aagctctcga acctccgcgt cgcctacggc gggcgcctca tcttccgcga cgaggaattc
     1621 ctcctcaggc ccggcgacaa gatcggcctc gtcgggccga acggcgcagg caagaccacc
     1681 ctcttcaggg tcctcgcggg cctggagaag ccggacggcg ggaccgtctc catggacccc
     1741 ggcacgacga taggctactt ctcccaggac gtcggcgaga tgtcggggag gagcgtcctg
     1801 gaggaggtcc tggccggggc cggccgcgtc cgcgagatcg ggactgagct cgaggccctg
     1861 gagcggcgca tggccgaccc ggaggacagg gggctcaccg attccgagat ggaccgctac
     1921 ggggagctcc agcacgagtt cgcccaccgc ggcggctacg acctggagcc ccgggcggag
     1981 gccatcctct ccggcctcgg cttcgagcgc gcgcgcttcg cggagcccgt ggagaatttc
     2041 tccggcggct ggaagatgcg gatcgccctc gcgcgcatcc tcctcctcga gccctccgtc
     2101 ctcctcatgg acgagcccac gaaccacctg gacctggagt ccatcgtctg gctggaggcc
     2161 tggctcaagg ccttccaggg cgacctcgtg atgacgagcc acgaccggga gttcatgacg
     2221 cggatctgcg gccgcaccgt ggaggtggcc cagggcgcgg tcacgaccta ctcgggcgac
     2281 tacgacttct acctccgcga gcgggagatc cgccgcgagc agctcgtggc ctcgcagaag
     2341 cgccaggagg ccatgctcgc caaggaggag gagttcatcg cgcgcttcgc cgcccgggcc
     2401 tcccacgcgg cccaggtcca gtcccgcgtg aagaccatag agaagatcga gcgcatcgtc
     2461 gtccccgccg accccaaggc gatcaaggtc cgcttcgagg actgcccgcg gagcggcgac
     2521 atcgtcgtcc agatggaggg gctcgcgaag tcctggccgg ctcccggcgg cggctcgcgg
     2581 gcggtcttcg agggcgcgac gggcgtcgtc cgccgccgcg acaaggtggc cctggtgggc
     2641 gtgaacggcg cgggcaagtc caccctcctc aaggtggtcg ccggcctcgc cgagccgagc
     2701 gccgggacct gctccctggg gccgagcgtg cgcctgggct acttcagcca gtactcgagc
     2761 gacgtcctcg tcccctcgcg gacgatcttc gaggaggtga aggagcggct gccccgggcc
     2821 tccgacggcc aggtcaagaa catcctgggc gccttccagt tcacgggcga cgacacggag
     2881 aagcgcgtct ccgtcctctc gggcggcgag aggagccgcg tcatgctcgc cctcatcctg
     2941 gccgagcccg tcaacttcct ggtcctcgac gagcccacga accacctcga catcgcgagc
     3001 cgcgaggtcc tcctggacgc gctctgcgag ttcgacggct cggtgatgat cgtgagccac
     3061 gaccgctact tcctcaggcg cctcgccacc cgcgtcttcg agatcgaccg gggctcgctg
     3121 tccgcctacg agggcgacta cggctactac ctcgagaaat cggcgagggt cagggccgag
     3181 ggcgccctcg tcgcggccgg agccggctga ggctcgccgc cggcggccag ggaggcgacg
     3241 atgcgcgtgg cccgccgcgc gagccgcggc gccccaagcc gggtccagcc gcgcggccga
     3301 ccgcgctccc ggcttgcatt cccggcggag tcgtgcgaga ttggaaaccc cgcgcctcaa
     3361 gcccgcggat ggaggatcct tgcggctctc cctagcctgt ctggccctgg ccttctcgct
     3421 cgccgccccg cccctcgcct cggccgctcc cgcggcctca gccgccgcgc ccgcgggaga
     3481 gcggatcctc gtcggctact atgacgcgcg tccctcctgc tggcgcggcg aggacggcga
     3541 ggccaagggc atcttcgtcg acatcctgaa ggaagtcgcg gtccgcgagg gctggagcct
     3601 cgaattctcc taccagggct gggacgggct cctcgaggcc ctcaagtccg gctcggtcga
     3661 cctcgtgccg gccatcgccc ggagcgagcc gcgctcctcc tacgccgcct tcacggagga
     3721 gacgatcttc ctcgactggg gggcggtctt cgtgggaagg agcggcaaga tccggtccat
     3781 cctcgacctg ggaggccggc gcgtcggcgc cctcgagaac gacatctggt tctccgggcc
     3841 gggagccctg cgcgacctct gcgcctcctt cctcgtcgag cccgactacg tcttcttccc
     3901 cgactacagc tccctcttcc gcgcgctggg ctcgggcgcg atcgacgcgg ccgccggcag
     3961 caactccctg ggcgtctact gggagtcgat ggagcccata gcggccacgc ccatcgtcta
     4021 ccttcccctc gagctccgcg tcgcggggcc ggcccgcggc tcccgcggct cccgcctggt
     4081 cggggcggtg gaccgccgag tgcgcgagct caaggaggag acccccggag ccctgcgctc
     4141 cgtcctggag cgctatgccg tgcccgcgcg ccgcgagtac gtcaccccgc cctgggtcgt
     4201 cccctccctc ctcctcctcg cggccttcct ggccctcatc gtcctcctcc tccttctcca
     4261 ggccgcggcc ctccggcgct cgcacgccga agcggccgcc gccctggcgg gcctcgagga
     4321 ggcgcacgga agcctcgagt cctccttcaa ggagaaggac ctcctcctcc acgagctctc
     4381 ccaccgggtg aagaacaact tccagctcat cctgagcctc atctccatgg cccaggcggg
     4441 cgaggaggag gagcgcggcg gcagcctggc cgagatcagg gagaagatct acgccctctc
     4501 cgtggcggag gagctcctcc acggcggcgg gggcgtggac gaggcggcgg tccgcgcctt
     4561 cgtggagcgg ctcgcctcca ggctctccga gctctacggc gcgagccggg aggacctgga
     4621 gatctcggtc cgcctcgggc ggccctacat tgacccgagc gcggccacgc ccctcgccca
     4681 agccctgagc gagcttgcgg ccaacgcctg caggcacggg cgcggagagg acgggcgggc
     4741 ctccctgtcc atcgccatcg agtcggctcc cgacggctcg gggagcatcg tcgtgtccga
     4801 ccgcgggccg ggcctccctc cgggcttcag cctcgcgcgc gacgcgcgcc tgggcctcgg
     4861 actcgtaagc gccctcgccg cccagctccg cgggcgcctg gaggccgggc ccggaccagg
     4921 aggcaggggc gcgtccttcg cgatccgcct ccccgccgaa tactggctcg cgcccgaagc
     4981 gaggcccgcg cccggagcga aaccttagcc cgaagcgaaa ccttagctcg gcgcgaagcc
     5041 cgcggcgcaa gccaagccag cgccctagga gcctgtccga gaaacctcgc acgccgcatg
     5101 cacttgccga ttcggctcgc caaggctcgc cgaatccgcc ctgtgttcat cgcgcctctc
     5161 tgttcatgcc ttcaccatcg atttccggac aggctcctag ccgatcctct cgagcgagct
     5221 cacggcgagg gtgatcccgc ccctgtcctc ctcgaccttg ccgcgcacca tgaggggcct
     5281 ctcctcgagg aggaggcgcc tatagccgcg gtaggcctcg ggaaagagga cggtctcgta
     5341 gagggccgtc tcgtcctcga aggagacgaa ctccatcggc tcctcgcccg cggccaggac
     5401 cgtcttcccc gcgatgggcc agcccacgag ctcgatccgc ctgccgacca aggaggaaag
     5461 gtccttcgcg agggagcgcc ttcgcgagag ggcttcgggc cagagggcca ggggatggac
     5521 ggccagggtc gtcccgaggt agcgcatctc cgcctccagg cgccgccgct ccgtctcctt
     5581 ggaggcgcgg cggggaaggg cgggtccctc gtcggggaag aggccggggc cctcgccctc
     5641 ctccctcgtg gccgccgcgg agagtaggcg catgagcttg tccgcgcggg agaggcccgg
     5701 agagagggag tcgagggcgc ccgagcccgc gagggcctcg gcgtcggacc tgtccggccg
     5761 cgcccgcctc gccaggtcct cgacggagcg gtaggggccg cgcgccttcc tctcctcgac
     5821 gaggcgcgag gccgcggcct ggcccaggcc cgcgaccatg ccgagtccga agcggagccc
     5881 gctcccgcgg ccgcggcagc ggagcccgct ctcgttcgcg tcggggccga gcagcccaag
     5941 gcccatccgc ctcgcctcgc tcgcgtaggc cagggtcgag tagtagccgc cgcggttcga
     6001 gatgacggcc gccatgaact ccgcggggtg gtggcggcgg aggaaggccg acttgaagga
     6061 gagcatcgcg tagctcgccg agtgggcctt cacgaaggag tagccggaga aggactcgat
     6121 catcgcccag acctcggcga ccgtcccttc gtccacgccg cgctcccgcg cccccgcctc
     6181 gaacctcgcc ctgaaggagg cgagcctctc cttcgcgtcc ttcttggaga ggaccttccg
     6241 catcccgtcg gcctcggcgg cgccgaagcc ggccagggcg atcgccgcct tgcagacgtc
     6301 ctcctggtag cacatgatgc cgaaggtctc cgccaggacg ccgtccagga ggggatggat
     6361 gggggcgtag ggcttgccgc ccaggcggtc gatgtactcg tcgatgtagc ggttggcggc
     6421 gggcctgatc atcgaggagt ggatgacgag gtgctcgaag tcgcccctgc ccgtcttctt
     6481 ctggaggagg cgcatcgccg ggctctccac gtagaagacg cccatcgtgt cgccccggcc
     6541 gagcatctcg atggtcgcct cgtcctcgag gggcttccac gcgtcctcgt cgatgccctc
     6601 gccgttctcc gcgaggttcg cgagggcgtc gcgcacgacg gccagggagc ggttccccag
     6661 gaggtcgatc ttcacgaggc cggcggcctc gacgccctcc ttgtcccagg ccgtgaccct
     6721 ggtccccgag cccgtccgct ccaggggcac gatggccgaa agagggccgg gcgcgacgac
     6781 gaggccgccc gagtggacgc ccaggtgccg cggcaggccg acgaggcggg gggccaggcg
     6841 ggctatagcc cgccagtcgt cgcaggcccc ctccagggcc gccgcctctc cgtccgccgc
     6901 ggccctgcgc tcgaaaccgg agatctcgcc ctccggcatg ccgtaggcgc gggccgcctc
     6961 gcggagggcc gagcgctcct taaagcgcac gtgattggcg acgcgggccg ccctgtcctc
     7021 gccgaagtcc gcgaggacct cggccaggag ggcgtcgcgc tcgtcccagg cgaagtcgac
     7081 gtcgatgtcc ggcgggtcga ggcggccggg gttgaggaag cgctcgaagt agaggccgtg
     7141 gcggatcggg tccacgtcgg tgatgcccag ggcgtaggag acgacgctcg ccgccgcgct
     7201 gccccggccg caggtcctcg aggcccgccc cgcgatgtcg cgcacgacca ggaagtagtc
     7261 ggagaagccc ttctcctcga tgatcccgag ctcgtagtcg atccgcgcga ggacctcggg
     7321 gcccgccttc ccgtagcgcc gctccgcccc ttccaggacg aggcggcgga ggagggccgc
     7381 ggcgccgccg cactcctcag gggcgccgcc cgaggcgccg gcctccgagg cgcccctgcc
     7441 ggcgccgccg cctgcgtcgc tcgagtcgtc cgccccgccg cccgcgcggc cagaaccgcg
     7501 gcccgcgccg cccccgccac gctcaacggc gccggcgcca tccgcgagag cgcagccctg
     7561 ccggcgcggg aagatccagc cgccaaagag gctcgaaagc ccgagcgact cgacggcctc
     7621 ctcgttggcc gcgagggcct cggggacttc ggcgtaggca gcctcgaagg ccgcgggcga
     7681 aaggaggagg gcgcccggat cggagagctc cgcctccccg acctcggcga ccgtcttccc
     7741 tccggcgatc gcgaggagga ggcgctggag ttccctgtcc ccggcctcga ggaagcggac
     7801 ctcgcccgtc gcgaggggcc tcgcccccgt gcggaggagg gcccgccagc ggggccggtt
     7861 gcagggcgag aggagggcgt ggaggcggcc gcccgaagcg ccgcccgagg cgctctccga
     7921 gcctgccgcc gcgccgccta acgcgcctac cgaagcgccg cccgaggcgc agcggtccgc
     7981 gaggccgccc gcggccaggg agaggagggc ttcgtcgtcc gtcgcggcga cgaggccccg
     8041 cgtatcgtcg gcgagctcgc gcctcgcgtc cagccttccc tcggcgcggg ccgtgaggag
     8101 gcgcgatatg cgcgcgaatc cctccctgtc cttcgcgagg aggagggccc ggccgccggg
     8161 ccaggagagc tccgtcccca tgatgggacg gcgcccctcg gcctcggcgg cctcgacgaa
     8221 ggcggggagg ccgtagacgc cgttcctgtc cgagagggcc agggcgccgt agccgagctc
     8281 cctcgcccgc ctcgcgaggg cggcgggcaa gacggcgccg aagcccaggg agtaggcgga
     8341 ggagacgagg agcgggacgc gcatgcgggg cctctaggcc ccggccccta gggccaggag
     8401 ggcgccgggg gagagggcct tggcgccgta cttgcccctg atcctgtcca gggccggctg
     8461 gagcctcgag agccgcgagt cctcgggctc gaagaggtcg agctcgggcc cggccgggac
     8521 gaattccgag agctcgaggc agaggcgcct gacgcggacc cgccgtttcc gcgcccgggc
     8581 cagggcggcc agggccaggt cgaggagctc gtcgtcgcgg gcccggaggc ccggggcgcg
     8641 ggccgagccc gagccccgct ccccgtcggc gtagacgagg gacaccgtgg ccctcgccgc
     8701 gcccgagccg ccggcccgca gggagaagcc gaggccggcg gcgagggcgc ccaggcggag
     8761 gcgcaggacc tcagggtcgc tcgtgtccgg ctcgaggagg gcttcgccgc ggagggagcg
     8821 ccggcccggg ggctcggggt cgacggggga ggcgtccagg cccctcgccc tggccgcgag
     8881 ctcgggcccc ctgggtccca gggccagggc ctccggccgc gagaggccgg cgagttcgcc
     8941 tatctcgtcg attccgagga ggaggagtcg ggagcggagg acggggccga cgcccgggag
     9001 gaggccgacg ggctggcgcc cgaggaggag ggactcctcg ccgggggaga gggcgacgaa
     9061 gccccctggc ctgaagaccc tggtcgcgac cttggagacc gtcttgttgg aggcgagggc
     9121 caggcagggc cggaggcccg tctcctccgc catcctcgcg cggaggcgct gggccgcgtc
     9181 ctcgggcggg ccgaagaggc ggcccgtgcc cgcgaggtcg acgaagaggt ggcccgcgcc
     9241 ggcccgctcg acgagggggc tgaactccag ggcggcgcgc gcgagccgct cgtcggcgag
     9301 gtcgcggagc tcgggccgcg gcggccgcgc cgcgaggccc gggcagagga gccgcgcctc
     9361 cttcagggcc atgccgcgcc tgagaccctc gcggtgggcc tcgggcgaga ggtccaggac
     9421 gatggcgcgg ggcgccccct cgttggccac gacgaagggc ctgcccttga ggctcccgtc
     9481 gaggccctcc tctatcgcgg ccaggaggcc gacggcgttg acgtggagga tgctgcccga
     9541 ggccatgccc gcgcccccct tttaggcgag cctcgcggcc ttggcgaggc gggagagcgc
     9601 gcgcgcgttg tccgccgcgt cccccctgcg gtggttgttg aagaagacga ggagcttcga
     9661 ggcctcggcg ctcatcgcct cgagcctcgg gatccaggcc ccgagctcct cgggcgagta
     9721 gcggtactcg tagcgcgagc ctgagtcccc cgtccaccag gcggcggcgt tgcggccgtg
     9781 gaagcgcacg taggcgagct cggcggtgac gaggtcggag agcggcggaa ggccctccag
     9841 gcggggcagg tccacggagc agagggcgac gcgccgctcc ttcaggccct cgatgacgcg
     9901 ggcgccgtac cattcggcgt tgcggaactc gacggcgagg ggaaaggagg agagctcccc
     9961 gaggagcctc cctaggtagc gccgctcctc ttccttgtag tggaaggaga aggggaactc
    10021 gaggaggacg gcggccaggc ggccggcctc gaggaggggg gcggcgcccc gggcgaattc
    10081 gagggcgtcg tccttccatg tggcaggatc gaccttgtgg gtcagggcct ggttggcctt
    10141 gatggagaag tcgatgggcc gcctcgtcct tccgagcatg ccgcggatct gctcggcctt
    10201 gggcatcccg tagtaggaga agttgagctc gaccgcgtcg aagacctcgc tgtaggcgcc
    10261 caggtactcc ttccgcccga ggcccttggg gtagaggacc ccctcccatt cggggtagtc
    10321 gtagcccgag gtgccgatcc tcagggcgca ggcgggcggg ggaaggctgc tcatgcccta
    10381 gttgtactac acgcgcgtgt agtaatcaag ggagttcccc gccccggcgc cgtttgcggg
    10441 cgcggccccc gccgcctatt cgacgagttc gtcctcaccc tcgaggacga ggtccccgtc
    10501 ctcccaggcc cgcttcaggt aggccaggaa ctcccgggcg gcctcggccg cgtcctcgcg
    10561 gcggacggcg cccaggacgg cgtactcgtc caggacgagg gcgcgccttc cctgggagac
    10621 gctgccgagg atcttcgcct tgaagtcctc gccgcggccc ttgaggagga gggccaggtc
    10681 gcggtcctgg aagtcgcgca gggccttctg caggtacttc cccggaaccc ggaggacgtc
    10741 gtccagggtg aagagccgct cgcggacgtc cttggagagc tcggggctct cgtcgtccag
    10801 ggcgtcgagg acgcgtccct ccaggcgggg gtcgacgtgg cggaggatgc cggccagggc
    10861 cgccctgccg tcgatctcct cgacggagac ggtgcctatc ttcctgatcc tgtccttgag
    10921 gccctcctcg acgcggcgga tgatctccgg gctcaccttc tcgaggcggg cgatgcgctt
    10981 cacgagctcg atgcggagct cctcgggcag gcgctccagg aggccggagg cccgccgggg
    11041 ctccaggtag gggaggatga ccgcgaggac ctggggcgac tcctccttga ggatgagctg
    11101 gagctcgcgg ggctcgaagt cgttgaggaa gcggaagggc cgcaaggtct cgggcgccgc
    11161 cttccgcatg aggtcgcggg cccggtcggg gccgaaggcg gcggtgagca tcttctccgc
    11221 ggtctcgggc ccgccctcga tggcgtagcc cttggtcttc acgagccagc cgaactccgc
    11281 gaggatctcg ttcgcctcga tggcgtcgat gctcttcacc tggatgatct cgcgggagag
    11341 ctcctctatc tccttggcgc ccaggtgggc caggaccttg gaggcctcct ccgagccgag
    11401 gaggaggagg aacttggccg ccttcgagat tccgcgctcg tcgccggtct tgaggaggcg
    11461 ctcggcctgg ccctccgcct tggccagggc cttgggcgtc ctggcctccg gacggcgggg
    11521 ctccgcggtc ggccggcggg ccgcgtccgc cgcctcccgg ggagcggcct ctcggggcgc
    11581 cgcctcgcgg cgaggggcgg gtcgggcggc ggcgggcccg cgcggggccg cctcggggag
    11641 gcgccgtccc tcgccgggta ggcgcggggc ctcctcggcc tcgcggtcgg gcgagggctt
    11701 gcccttgtag gcgcgctcga tgcggtcctt catgttcatg ccgccatcct agccgcgggc
    11761 aggacccgag tcaatcgaag ccgcccccgg ccggggcagg gccagggtca atcgaagccg
    11821 gccaggggcg ccgtcgccgc gggcgaagag ggcccgaggg agcgcctcgc gagctcgagg
    11881 cgcagcttga actcggcctc catgagctcg gggaggggca ggggctcgct gaggcggggg
    11941 gggaggggga tgccggcctc gtcggtgac
//
LOCUS       DAOOSJ010000052        11830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3614_length_11830_cov_7.150064, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000052 DAOOSJ010000000
VERSION     DAOOSJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_11830_cov_7.150064"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLG14_04095"
     CDS             <1..428
                     /locus_tag="PLG14_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48345.1"
                     /translation="LEAMFKEAARVLAPGGLFAVASQSHAQIAARPVARFFPETVAAD
                     RARYPDSGRLAAAAAPAGFAFLGDEAYGEGEPVVLDESFLELAERKGYSVLAAIGEEA
                     YRKGLAALRAALAESVREGRPLELPAAGGTVHWFAKAAR"
     gene            complement(547..1944)
                     /locus_tag="PLG14_04100"
     CDS             complement(547..1944)
                     /locus_tag="PLG14_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOX48346.1"
                     /translation="MELPYAEPYRIKTIETIRRSTRKEREAWIAEAKYNLFNLRSEQV
                     YVDLLTDSGTGAMSSAQWAEMMLGDESYAGASSYYKLKDSVKEVMGFDWFLPAHQGRA
                     AENVLMSALIGTGHEGRGKVVPGNSHFDTTKGHIESRGAHAVDCTVDEAFDTTLERPF
                     KGNVDLSKLEKVFKEHKREDIPLCILTVTCNTSGGQPVSMENVKGVSRLCRKYGVPLF
                     LDAARFAENCYFIKTREKGYARKDIRDIAREMFSYADGATMSSKKDGIVNIGGLILFR
                     EEEMWRRCSTFNICYEGFNTYGGMAGRDLGALSVGLKEATEFDYLESRIGQVAYLGRK
                     LTDYGVPVMLPYGGHAIFVDAKRFLPKVPKEEFVAQTLGVEAYLEGGVRGVEIGAILA
                     DRDPDTRENRYPALELLRLAIPRRTYTNAHMDYVAAALANVFERRDSIKRGYVIKKEA
                     PLLRHFTVELAPAKK"
     assembly_gap    1965..2064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2065..3837
                     /locus_tag="PLG14_04105"
     CDS             <2065..3837
                     /locus_tag="PLG14_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48347.1"
                     /translation="LWRYYKPYKWILFTDLFAVVAGAAFGLAVPYLLKILVDRAVPSR
                     DSGALLAVAATVAVLALARYAANYYSLYMGHALAARMERDMRRDLFRKIEGMSFSFFD
                     ERKTGELMSRMINDVGKVSDAVNHAPEDLLLAAFTIAGSYAVLFSLHPPLALVSLVPL
                     PLMVLYSGLLGGRILKGFERANDAVAEVNAKVENIVAGIRVVQSFAREEEEERRFASL
                     NEENYRAWNSVLRTLGWYFGGVDLMRDLSRLLIVAAGGWFAIQGSLSLGAFVAFVSYV
                     GIYLEPIERLTRTVESIQRMAAGLKSFFAIMDEESAVRDAPGARELPPAVLGGICFEG
                     VTFSYGGDRHVFRELDLGIAPGAKVALVGPSGAGKTTFCNLIPRFYEPQSGRVLLDGV
                     DVREYRLKELRRAVGIVQQDVFLFAGTVFENIAYGKPGASREEVERAARDANAAEFVE
                     ALPKGYDTEIGERGVKLSGGQKQRLAIARAFLKDPPVLVLDEATSSLDAKSERAVQEA
                     IARLARGRTTLIIAHRLSTVREADEILVLTEEGLAQRGRHEDLVREAGPYAELYAAQG
                     AGILRDEEGGDGEGEAEEEAEAAL"
     gene            3872..5116
                     /locus_tag="PLG14_04110"
     CDS             3872..5116
                     /locus_tag="PLG14_04110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48348.1"
                     /translation="MRDWKTSFRALWIAEFIAITGFQTSNPILPLYLGELGLSDPQAV
                     YWWTGAINSSAAIALAVFAPIWGAVADGYGRRLMLLRAMVGGTILVGLMAFAREPWQI
                     LALKTLQGCVTGTVAAATVLTASIVPAKEAGYRLGLLQMAVFLGNSAGPLFGGVVADL
                     AGARFTFLATSALLAFSVYIVLTRVEEKFTPKPKSKSLLRNALPDFSVLAKSPALASL
                     LFVTFSVQLANMVVGPIIPLIVKELAGGGAGIASLSGLIIAASSMAGALAAVVVGKIS
                     ARLGYGRALLLCLGGAFVFYLPQGLVRSPYALLALRVGSGFFLGGTMPSVNALIASLC
                     DRDKQGSTYGLSASVSSAGGAIGPALGAVVATSAGNAAVFYATSAILALTGLAVGAGV
                     RARAKGPHGGSADGAPTGGAPA"
     gene            5269..5778
                     /locus_tag="PLG14_04115"
     CDS             5269..5778
                     /locus_tag="PLG14_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48349.1"
                     /translation="MRIERRESADDGARGGDARLERALKVAAAALAVAILLGTAYGLA
                     TGSRGKKLAREAEAALAAPALAEGSAVFKGLGTVRASTRDEPKAVVVATLSFPYPASE
                     RAFREELARKAPALKAAAVAYLSRRSAAELHPAYEGAVKAGLRDALNELLSLGKVEEL
                     WMSDFAVIK"
     gene            complement(5786..6451)
                     /locus_tag="PLG14_04120"
     CDS             complement(5786..6451)
                     /locus_tag="PLG14_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX48350.1"
                     /translation="MENSDGFFLERARDFAAARHEGSGPCHDFSHVERVYRLAERIAR
                     SEGADLLTVRLAALLHDIGRGLEPRIGPEPDRHEELSAQIAAPFLRGLGLPEARVEAV
                     LEAILRHRHRRGRAPESLEARCLYDADKLDSLGAVGAARAYLWLGEHGRSVHYPEAEW
                     AGVDPASNATEVDSFQREWRIKLSRLKDRLYTEEGRRLAGERHARMERILMEIEDEVA
                     GRS"
     gene            6570..7829
                     /gene="pepT"
                     /locus_tag="PLG14_04125"
     CDS             6570..7829
                     /gene="pepT"
                     /locus_tag="PLG14_04125"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOX48351.1"
                     /translation="MKSYADILSKPIAEKVAERLVRYAKIGTTSDRHVEAIPSTQTQW
                     ELSRLLVEELKGLGLKDVTLDEHCYLLARLPASPGLESAPSIGFMAHVDTASDVSGTD
                     VKPRLVKGYDGKALELSPGYVLDPREFPDLAEHAGDTIIVTDGRTLLGADDKAGVAEI
                     VTAVEWLLAHPEVKHGPIEIAFTPDEETGKGMDLFPLKNLRSVACYTFDGGKAGEVEA
                     ECFNAYEVKAEFTGKAIHIGSARGKLANAVTMAASFVSMLPWSEAPESTDGWFGYFCP
                     LEIKGGIEKASVDVFLRDFSKEGMDRRIETCKALAKAVEAQFPLGSVKLEVNKQYVNM
                     REKLDARPEVLERAMEASRRAGVEPFVKPIRGGTDGARLTELGVPTPNLFTGGYNYHS
                     RFEWASVSEMALATETVVELAKLWATK"
     gene            complement(8170..9357)
                     /locus_tag="PLG14_04130"
     CDS             complement(8170..9357)
                     /locus_tag="PLG14_04130"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOX48352.1"
                     /translation="MIMKEVFIADAARTAVGSFGGSLASVSPVELGRTVVEALLKRGS
                     LQGADVDELLLGCVLQASLGQNVARQILVKSGIPVEKTAMTINMVCGSGLRSVAAAAQ
                     EIKAGDANLVVAGGVENMSLAPYYLKQARTGYRMGNGEMVDGMIYDGLWDIFNGYHMG
                     ITAENVAAKYGITRAEQDAFSAASQNKAEKAIAEGRFKDEIVPVAIPQKKGDPIVFAT
                     DEFVRAGVTAEALAKLRPAFKPDGTVTAGNASGINDGAAAILVADSDGMKRGKLAPMA
                     RIASYGWHGCDPAIMGVGPVEAVRLALRRAGWKLEDVDLIEANEAFAAQSLAVAKELK
                     FDMARVNVNGGAIAIGHPIGASGARILTSLLYEMKKRGAKKGLATLCIGGGMGVAMCV
                     ERV"
     gene            complement(9471..10133)
                     /locus_tag="PLG14_04135"
     CDS             complement(9471..10133)
                     /locus_tag="PLG14_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOX48353.1"
                     /translation="MEYQENKDIIAKRIALMLKTGDVVNLGIGLPTLVANYVPSDVVV
                     ILQSENGLIGLGPAPEKGKENKDLTNAGAQPVTILPGGAFFDSATSFGIIRGGHVDAT
                     VLGVLEVDQEGNLANYKIPGKMVPGMGGAMDLVAGSRVVIAATTHFEKSGAAKLKKRC
                     TLPLTAAHEVDYVVTDLGLFHVEKGAFVLKEYFAPYTPEWIKEKTDANVVIAPDCKLF
                     QA"
     gene            complement(10145..10816)
                     /locus_tag="PLG14_04140"
     CDS             complement(10145..10816)
                     /locus_tag="PLG14_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOX48354.1"
                     /translation="MTQKPLIAAAEAARLVKDGMTVHVGGFLGCGSPERIIAALDAQG
                     TKDLTLVCNDTAVLDLKNGRENGVSVLVKNKRFKRMIVSHIGTNPETQRQMNAGEAVV
                     DLVPQGTLAERIRAAGVGLGGVLTPTGVGTEVEQGKQKLSVGGKDYLLEAALPGDIAL
                     IKAKKADKAGNLVYSMTARNFNPLMATACAVVVAEVEEIVEIGEIDPDQVHTPSIFVD
                     YLVKA"
     gene            11053..>11830
                     /locus_tag="PLG14_04145"
     CDS             11053..>11830
                     /locus_tag="PLG14_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HOX48355.1"
                     /translation="MKGLTRFFVKVSQKYLPDAFLFAIILTVIAFGLALGLTGKTVIQ
                     VLAAWGGGLWGILAFAMQMVLVLVTGHALASSPAIKRGLVAISSVAKTPVQGLMLTSF
                     VTAIACWLNWGFGLIVGALLAREVAKRVRGIDYGMLVAAAYSGFVVWHGGISGSIPLA
                     IASKGHIVENLIGIVPVSRTIFAPFNLIISFAVIFALPILFMFMAKGKTDVVSIDPAL
                     LADEPIPEEDRKRSPAARLENSRIVNWVFGLMGVAYLVYHF"
BASE COUNT         1646 a         4216 c         3934 g         1934 t
ORIGIN      
        1 acctggaggc gatgttcaag gaggcggcgc gcgtcctcgc gcccgggggc ctcttcgccg
       61 tcgcgagcca gagccacgcc cagatcgcgg cccgccccgt cgcgcgcttc ttccccgaga
      121 cggtcgcggc cgaccgggcg cgttatccgg actcagggcg gctcgcggcc gctgcggctc
      181 ccgccggctt cgccttcctg ggcgacgagg cctacggcga gggcgagccc gtcgtcctgg
      241 acgagagttt cctcgagctc gcggagagga agggctactc ggtgctcgcg gccatcggcg
      301 aggaggccta ccggaagggg ctcgccgcgc tccgcgccgc cttggcggag tcggtccgcg
      361 aggggcggcc cctcgagctc cccgcagccg ggggcacggt ccactggttc gcgaaggccg
      421 cccgctaggg cgggccgcgg ccaaatgaaa ggggccgcca ggaatcgctt ccttggcggc
      481 cccttgtccg cgagcttcgc ggccccgtct acttcttcgc gggcgcgggt cagcggagag
      541 cgcgccttac ttcttcgcgg gcgcgagctc gacggtgaag tggcggagga ggggggcctc
      601 cttcttgatg acgtagccgc gcttgatcga gtcgcggcgc tcgaagacgt tcgcgagggc
      661 cgcggcgacg tagtccatgt gcgcgttcgt gtaggtgcgg cgggggatgg cgagcctgag
      721 gagctcgagg gccgggtagc ggttctcgcg cgtgtcggga tcgcggtcgg ccaggatggc
      781 gccgatctcc acgccgcgga cgccgccctc caggtaggcc tcgacgccga gggtctgggc
      841 gacgaactcc tccttcggga ccttgggcag gaagcgcttc gcgtccacga agatcgcgtg
      901 cccgccgtag ggaagcatga cggggacgcc gtagtccgtg agcttccgcc ccaggtaggc
      961 gacctggccg atccgggact cgagatagtc gaactcggtg gcctccttca ggccgacgga
     1021 gagggcgccc aggtcgcggc ccgccatgcc gccgtaggtg ttgaagccct cgtagcagat
     1081 gttgaaggtg gagcagcggc gccacatctc ctcctcgcgg aagaggatga ggccgccgat
     1141 gttcacgatg ccgtccttct tgctcgacat cgtcgcgccg tcggcgtagg agaacatctc
     1201 ccgcgcgatg tcgcggatgt ccttcctcgc gtagcccttc tcccgggtct tgatgaagta
     1261 gcagttctcc gcgaagcggg ccgcgtccag gaagaggggc acgccgtact tcctgcagag
     1321 cctggagacg cccttgacgt tctccatgga gacgggctgg ccgccggagg tgttgcaggt
     1381 gacggtgagg atgcacagcg ggatgtcctc gcgcttgtgc tccttgaaga ccttctcgag
     1441 cttcgaaagg tcgacgttgc ccttgaaggg ccgctccagc gtcgtgtcga aggcctcgtc
     1501 cacggtgcag tcgacggcgt gggcgccgcg gctctcgatg tggcccttgg tcgtgtcgaa
     1561 gtgggagttg ccgggcacga ccttgcccct gccctcgtgg cccgtgccga tgagggcgga
     1621 catgaggacg ttctccgcgg cgcggccctg gtgggcgggc aggaaccagt cgaagcccat
     1681 gacctccttc acggagtcct tgagcttgta gtaggaggag gcgccggcgt agctctcgtc
     1741 gccgagcatc atctccgccc actgagctga ggacatcgcg ccggtgccgg agtcggtgag
     1801 gaggtcgacg tagacctgct cgctcctgag gttgaagagg ttgtatttcg cctcggcgat
     1861 ccaggcttcg cgctccttgc gcgtcgaccg cctgatcgtc tcgatggtct tgatcctgta
     1921 gggctcggcg tagggcagtt ccatgggttc ctccgttagt atggnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnctctgg cgctactaca agccctacaa gtggatcctc
     2101 ttcaccgacc tcttcgccgt ggtcgcgggc gccgccttcg gcctcgccgt gccctacctc
     2161 ctcaagatcc tcgtggacag ggccgtgccc tcgcgcgact cgggcgccct cctcgccgtc
     2221 gccgcgaccg tggccgtcct ggccctcgcg cgctacgcgg ccaactacta cagcctctac
     2281 atgggccacg ccctggccgc caggatggag cgcgacatgc gccgcgacct cttccggaag
     2341 atcgagggga tgagcttctc cttcttcgac gagcggaaga ccggcgagct catgtcgagg
     2401 atgatcaacg acgtgggcaa ggtctccgac gcggtgaacc acgcgccgga ggacctcctc
     2461 ctcgccgcct tcacgatcgc gggctcctac gccgtcctct tctccctcca cccgcccctc
     2521 gccctcgtct ccctcgtccc cctccccctc atggtcctct actcgggcct cctgggcggc
     2581 aggatcctca agggcttcga gcgcgcgaac gacgcggtcg cggaggtgaa cgcgaaggtg
     2641 gagaacatcg tcgcgggcat ccgcgtcgtg cagtccttcg cccgcgagga ggaggaggag
     2701 cggcgcttcg cctcgctcaa cgaggagaac taccgcgcct ggaactcggt cctgcggacc
     2761 ctgggctggt acttcggcgg cgtcgacctc atgcgcgacc tctcgcgcct cctcatcgtc
     2821 gcggccggcg gctggttcgc gatccagggc tccctgagcc tgggcgcctt cgtggccttc
     2881 gtctcctacg tcggcatcta cctcgagccc atcgagcgcc tgacgcgcac ggtcgagtcg
     2941 atccagcgca tggccgccgg cctcaagagc ttcttcgcga tcatggacga ggagagcgcg
     3001 gtgcgcgacg cgcccggcgc gcgggagctc ccgcccgcgg tccttggcgg catctgcttc
     3061 gagggcgtga ccttctccta cggcggggac aggcacgtct tcagggagct cgacctcggg
     3121 atcgccccgg gggccaaggt ggccctggtc ggcccctcgg gcgccggcaa gacgaccttt
     3181 tgcaacctca tcccccgctt ctacgagccc cagtccggcc gcgtcctcct ggacggggtc
     3241 gacgtccgcg agtacaggct caaggagctc cgccgggccg tcggcatcgt ccagcaggac
     3301 gtcttcctct tcgccggcac cgtcttcgaa aacatcgcct acgggaagcc gggcgcctcg
     3361 cgggaggagg tggagcgcgc cgcccgggac gcgaacgcgg cggagttcgt cgaggccctg
     3421 cccaagggct acgacacgga gatcggcgag cggggcgtga agctctcggg cggccagaag
     3481 cagcgcctcg ccatcgcgcg tgccttcctc aaggaccctc ccgtcctcgt cctggacgag
     3541 gcgacgagct ccctggacgc gaagagcgag cgggcggtgc aggaggccat cgcgcggctc
     3601 gctcggggcc gcacgaccct catcatcgcc caccgcctct ccaccgtgcg cgaggccgac
     3661 gagatccttg tcctcacgga ggagggcctg gcccagcggg gccgccacga ggacctcgtc
     3721 cgcgaggcgg ggccctacgc cgagctctac gccgcccagg gcgcggggat cctccgcgac
     3781 gaggagggcg gcgacgggga gggcgaggcc gaggaggagg ccgaggcggc gttgtaatcg
     3841 aggggccctc cgcgctagga tggggcatcc tatgcgcgac tggaagactt ccttcagggc
     3901 gctctggata gccgagttca tcgcgatcac cggcttccag acctccaacc ccatcctgcc
     3961 cctctacctg ggcgagctcg gcctctccga cccccaggcg gtctactggt ggacgggcgc
     4021 gatcaactcc tccgccgcca tcgccctcgc ggtcttcgcg ccgatctggg gcgccgtcgc
     4081 cgacggctac ggcaggcgcc tcatgctcct ccgcgccatg gtcggcggga ccatcctcgt
     4141 gggcctcatg gccttcgcga gggagccctg gcagatcctc gccctcaaga ccctccaggg
     4201 ctgcgtcacg ggcaccgtcg ccgccgcgac cgtcctcacc gcgagcatcg tgccagccaa
     4261 ggaggcgggc taccggctcg gcctcctcca gatggcggtc ttcctgggga actcggccgg
     4321 ccccctcttc ggcggcgtcg tcgccgacct cgccggggcg cgcttcacct tcctcgccac
     4381 ctcggccctc ctcgccttct cggtctacat cgtcctcacc cgggtcgagg agaaattcac
     4441 cccgaagccc aagtcgaagt ccctcctcag gaacgccctc cccgacttct ccgtcctcgc
     4501 caagagcccg gccctcgcga gcctcctctt cgtcaccttc tccgtccagc tcgcgaacat
     4561 ggtcgtgggc cccatcatac ccctcatcgt caaggagctc gcgggaggcg gagcgggcat
     4621 cgcgtcgctc tcggggctca tcatcgcggc gagctcgatg gcgggagccc tcgcggcggt
     4681 ggtggtcggc aagatctcgg cgcggctcgg ctacggccgg gccctcctcc tctgcctggg
     4741 cggcgccttc gtcttctacc tgccgcaggg cctcgtccgc tccccctacg ccctcctcgc
     4801 cctgcgcgtc ggctcgggct tcttcctggg cggcaccatg ccgagcgtga acgccctcat
     4861 cgcgagcctc tgcgaccgcg acaagcaggg ctcgacctac gggctctcgg cctccgtctc
     4921 cagcgccggc ggcgcgatcg ggcccgccct cggcgccgtc gtcgcgacga gcgcgggcaa
     4981 cgccgcggtc ttctacgcga cgagcgccat cctggccctg acgggcctgg ccgtcggcgc
     5041 cggcgtgcgg gccagggcca agggtccgca cgggggctcc gcggacggcg ccccgacggg
     5101 cggcgccccg gcctgagcgg cgcgccttgg cctgcgtggc gcgcctccgc ctagcggctc
     5161 ctcgggcggc gccggaggcg gcgctcgcgg cgaccgcgcc gccgagggcg cgccgcgtcg
     5221 ggcgcgccgc acgttgactg gcccgtcccg ccgtcctaca atcggcccat gaggattgag
     5281 cgaagagaaa gcgccgatga cggcgcgcgc ggcggcgacg cgaggctcga gagggccctc
     5341 aaggtggccg cggccgcgct cgccgtcgcc atcctcctgg gtacggccta cggcctggcc
     5401 acggggagcc gcggcaagaa gctggcccgg gaagccgagg ccgccctggc ggcgccggcc
     5461 ctggccgagg gcagcgccgt cttcaagggc ctcgggacgg tcagggcctc cacgagggac
     5521 gagcccaagg ccgtcgtcgt cgcgaccctg agcttcccct accccgcctc cgagcgcgcc
     5581 ttccgcgagg agctcgccag gaaagcgccg gccctcaagg ccgcggccgt cgcctacctg
     5641 tcgcgccgct cggcggccga gctccatccc gcctacgaag gcgccgtcaa ggccggcctc
     5701 cgcgacgccc tcaacgagct cctttccctg ggcaaggtcg aggagctctg gatgtccgac
     5761 ttcgcggtga tcaagtagcc ggattctagc tccgccccgc cacctcgtcc tcgatctcca
     5821 tgagtatgcg ctccatccgc gcgtggcgct cccccgcgag gcgccggccc tcctccgtgt
     5881 agaggcggtc cttgagccgc gagagcttga tgcgccattc ccgctggaag gagtccacct
     5941 ccgtcgcgtt gctcgcagga tcgacgcccg cccactccgc ctccggatag tgtacggagc
     6001 ggccgtgctc gcctagccag aggtaggccc gcgccgcgcc caccgcgcca agggagtcga
     6061 gcttgtccgc gtcgtagagg cacctcgcct ccaggctctc cggcgcgcgg ccgcggcggt
     6121 gccggtggcg gaggatggcc tccaggacag cctcgaccct cgcctcgggc aggccgaggc
     6181 cgcggaggaa gggcgcggcg atctgcgcgg agagctcctc gtggcggtcg ggctcggggc
     6241 cgatgcgcgg ctcgaggccc cgtccgatgt cgtggagaag ggccgcgagg cggaccgtga
     6301 ggaggtcggc gccctcggag cgggcgatgc gctcggcgag gcggtagacc cgctcgacgt
     6361 gggagaagtc gtggcagggc cccgagccct cgtgcctggc cgccgcgaag tcccgcgctc
     6421 tttcaaggaa gaacccgtcg ctgttttcca tcgcggacaa ggatacgcgg cccggggccg
     6481 gggccgcaag cgccccgggc gaaggcggca gagacaggcg acggagatag cgcttgcccg
     6541 cgcggcgttt ccggtgcata ctctcggcca tgaaatccta cgccgacatc ctctccaagc
     6601 ccatagcgga aaaggtcgcc gagcgcctcg tgcgctacgc gaagatcggg acgacgagcg
     6661 accgccacgt cgaggccata ccctcgaccc agacccagtg ggagctctcg cgcctcctcg
     6721 tcgaggagct caagggcctc ggcctcaagg acgtgacgct cgacgagcac tgctacctcc
     6781 tcgcgcgcct ccccgcgagc cccggcctcg agtcggcgcc ctccatcggc ttcatggccc
     6841 acgtggacac ggcgagcgac gtctccggga cggacgtgaa gccccgcctc gtgaagggct
     6901 acgacggcaa ggccctcgag ctctctcccg gctacgtcct cgacccgagg gagttccccg
     6961 acctcgccga gcacgcgggc gacaccatca tcgtcaccga cggccggacc ctcctcggcg
     7021 ccgacgacaa ggccggcgtc gcggagatcg tgaccgccgt cgagtggctc ctcgcccacc
     7081 ccgaggtcaa gcacggcccg atcgagatcg ccttcacccc cgacgaggag acgggcaagg
     7141 gcatggacct cttcccgctc aagaacctgc gctccgtcgc ctgctacacc ttcgacggcg
     7201 gcaaggccgg cgaggtggag gcggagtgct tcaacgccta cgaggtcaag gccgagttca
     7261 cgggcaaggc catccacatc ggctcggccc gcggcaagct cgccaacgcc gtcacgatgg
     7321 ccgcctcctt cgtctccatg ctcccctgga gcgaggcgcc cgagtccacc gacggctggt
     7381 tcggctactt ctgccccctc gagatcaagg gcggcatcga gaaggcctcc gtcgacgtct
     7441 tcctccgcga cttctccaag gagggcatgg accgccgcat cgagacctgc aaggccctcg
     7501 cgaaggccgt ggaggcgcag tttcccctcg gctccgtcaa gctcgaggtc aacaagcagt
     7561 acgtcaacat gcgcgagaag ctcgacgcga ggcccgaggt cctggagcgc gcgatggagg
     7621 cctcccgccg cgccggcgtc gagcccttcg tgaagccgat ccgcggcggc accgacggcg
     7681 cccgcctcac cgagctcggc gtcccgacgc ccaacctctt cacgggcggc tacaactacc
     7741 acagccgctt cgagtgggcc tccgtctccg agatggccct cgccacggag accgtggtcg
     7801 agctcgccaa gctctgggcg actaagtagc cttcgcccgc ctttccccat aacgaggggc
     7861 ctcccgggaa tcgcttcccg ggaggcccct ctctcttgcc ccgcgtcgcc gccattacgc
     7921 ggcggcgcgg cgtccagcgc cgcgcttcac gcggcgaaag tccctaaacg cgctccacgc
     7981 acatcgcgac gcccacgacg ttgcccgcgg ctacgcgggc actacaatac tcttcccctc
     8041 gtcgccaagc gcgaagcgct tgatgccgcc gccgatgccg ccgcccgcgt tagcgggcac
     8101 gtcaatacct tccattcggc tccggcggcg gcgcagcgtc cagctccgcg cttcacgcgg
     8161 cgaaagtccc tatacgcgct ccacgcacat cgcgacgccc atgccgccgc cgatgcacag
     8221 ggtggcgagg cccttcttcg cgccccgctt cttcatctcg tagaggaggc tcgtaaggat
     8281 gcgggcgccg gaggcgccga tcgggtggcc gatcgcgatc gctccgccgt tcacgttgac
     8341 ccgggccatg tcgaacttga gctccttggc cacggccagg gactgggcgg cgaaggcctc
     8401 gttggcctcg atgaggtcca cgtcctcgag cttccagccc gcgcgcctga gcgcgaggcg
     8461 gacggcctcc acggggccga cgcccatgat cgcggggtcg cagccgtgcc agccgtagga
     8521 ggcgatgcgc gccatcggcg cgagcttgcc gcgcttcatg ccgtcggagt cggcgacgag
     8581 gatggcggcc gcgccgtcgt tgatgccgga ggcgttgccc gcggtcacgg tgccgtcggg
     8641 cttgaaggcg ggccggagct tggccagggc ctccgcggtc acgcccgcgc ggacgaactc
     8701 gtcggtcgcg aagacgatcg ggtcgccctt cttctggggg atggcgacgg gcacgatctc
     8761 gtccttgaag cggccctcgg cgatcgcctt ctcggccttg ttctgcgagg ccgcggagaa
     8821 ggcgtcctgc tcggcgcggg tgatgccgta cttcgcggcg acgttctccg ccgtgatgcc
     8881 catgtggtag ccgttgaaga tgtcccagag gccgtcgtag atcatgccgt ccaccatctc
     8941 gccgttgccc atgcggtagc cggtgcgggc ctgcttgagg tagtagggcg cgagactcat
     9001 gttctcgacg ccgcccgcga cgaccaggtt ggcgtcgccg gccttgatct cctgggcggc
     9061 ggccgcgacg gagcggaggc cggagccgca gaccatgttg atcgtcatcg cggtcttctc
     9121 gacggggatg ccggacttga cgaggatctg gcgggcgacg ttctggccca ggctggcctg
     9181 gaggacgcag ccgaggagga gctcgtcgac gtcggcgccc tggagggagc cgcgcttgag
     9241 gagcgcctcc acgaccgtgc ggccgagctc gacagggctg acggaggcga ggctcccgcc
     9301 gaaagagccg accgcggtgc gcgcggcgtc ggcgatgaaa acttccttca tgatgatgct
     9361 ccttgcgggg ccctgcccgc gggatccctc ccgccggcgg cccctcgacg cggcgccccg
     9421 ggccctcaag cgggcgcggg gcgtctggcg aactgccttc cctccggcct tcaggcctgg
     9481 aagagcttgc agtccggcgc gatcacgacg ttcgcgtcgg tcttttcctt gatccactcg
     9541 ggggtatagg gcgcgaagta ctccttgagc acgaaggcgc ctttctccac gtggaagagc
     9601 ccgaggtcgg tcacgacgta gtcgacctcg tgcgccgcgg tgagcgggag cgtgcagcgc
     9661 ttcttgagct tcgccgcgcc ggacttctcg aagtgggtgg tcgcggcgat gacgacgcgg
     9721 gagcccgcga ccaggtccat cgcgccgccc atgcccggga ccatcttgcc cgggatcttg
     9781 tagttggcga ggttcccctc ctggtcgacc tccaggacgc cgagcaccgt cgcgtcgaca
     9841 tggccgccgc ggatgatgcc gaagctcgtc gccgagtcga agaaggcgcc gcccggcagg
     9901 atggtgaccg gctgggcgcc cgcgttcgtg aggtccttgt tctccttgcc cttctcgggc
     9961 gcgggcccga ggccgatgag gccgttctcg ctctggagta tcacgacgac gtcggagggc
    10021 acgtagttgg ccacgagggt cggaaggccg atgccgaggt tcactacgtc gcccgtcttg
    10081 agcatgaggg cgatgcgctt cgcgatgatg tccttgttct cctggtattc catagtcccc
    10141 tcccttaggc cttcacgagg tagtcgacga agatggaggg cgtgtggacc tggtcgggat
    10201 cgatctcgcc gatctccacg atctcctcca cctcggcgac gacgaccgcg caggccgtcg
    10261 ccatgagggg gttgaagttg cgcgccgtca tcgaatagac gaggttgccg gccttgtcgg
    10321 ccttcttcgc cttgatgagg gcaatgtcgc cggggagggc cgcctcgagg aggtagtcct
    10381 tgccgccgac ggagagcttc tgcttgccct gctcgacctc cgtgccgacg cccgtcggag
    10441 tgaggacgcc gcccaggccc acgccggccg cgcggatgcg ctcggccagg gtgccctggg
    10501 gcacgaggtc gacgacggcc tcgccagcgt tcatctggcg ctgggtctcg ggattcgtgc
    10561 cgatgtgcga cacgatcatg cgcttgaagc gcttgttctt gacgaggacg gagacgccgt
    10621 tctcgcggcc gttcttgagg tccagcaccg ccgtgtcgtt gcagacgagg gtgagatcct
    10681 tggttccctg ggcgtctagg gccgcgatga ttctctcggg agatccgcag ccgaggaagc
    10741 cgccaacgtg gaccgtcatc ccgtccttca cgagcctcgc ggcttccgcc gccgctatga
    10801 ggggtttttg agtcactagg ctatcctccg gaaaaagatg aaagaagaaa ggccgtcctc
    10861 gggccattgc cttcgggcga tcgattcatt atagcattat tttataaact atgataaaca
    10921 tgttcaatat ctcaggcgca tttatatcta cgcccccgat gctttcaccg ggatccgtcg
    10981 ggatcccttg agagacgcgc ggccttcaaa ggccgcgcgg cgaagccata tcttttcatc
    11041 aggaggaaga ccttgaaagg tctaaccagg ttcttcgtaa aggtctcgca aaagtacctt
    11101 cccgacgcgt tcctcttcgc gatcatcctc accgtgatcg ccttcggact cgcccttggc
    11161 ctcaccggca agacggtcat ccaggtcctc gcggcctggg gcggcggcct ctggggcatc
    11221 ctcgccttcg cgatgcagat ggtcctcgtc ctcgtgaccg gccacgccct cgcctccagc
    11281 cccgcgatca agcgcggcct ggtcgcgatc tcctccgtgg ccaagacgcc cgtccagggc
    11341 ctcatgctca cgtccttcgt cacggcgatc gcctgctggc tcaactgggg cttcggcctc
    11401 atcgtcggtg ccctcctcgc ccgcgaggtc gcgaagcgcg tgcgcggcat cgactacggc
    11461 atgctcgtcg ccgctgccta ctcgggcttc gtcgtgtggc acggcggcat ctcgggctcc
    11521 atccccctcg ccatcgcctc caaggggcac atcgtcgaga acctgatcgg catcgtgccg
    11581 gtgagccgga cgatcttcgc gcccttcaac ctaatcatct ccttcgcggt gatcttcgcc
    11641 ctccccatcc tcttcatgtt catggccaag ggcaagaccg acgtcgtctc catcgacccc
    11701 gccctcctcg ccgacgagcc catccccgaa gaggaccgca agcgcagccc cgccgcgcgc
    11761 ctggagaact cccgcatcgt caactgggtc ttcggcctca tgggcgtcgc ctacctcgtc
    11821 taccacttcg
//
LOCUS       DAOOSJ010000053        11751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3632_length_11751_cov_5.758037, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000053 DAOOSJ010000000
VERSION     DAOOSJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11751
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_11751_cov_5.758037"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLG14_04150"
     CDS             complement(<1..359)
                     /locus_tag="PLG14_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOX48356.1"
                     /translation="MEREKIEERARDYVARETDAAFKAEVEKLLAAGDWKELEDRFYR
                     DLEFGTGGLRGVIGGGYNRMNSLVVTRATQGLCDYLRKTFPGKSLSACVAYDSRRYSD
                     VFAEMTARVFAANGVKA"
     gene            complement(463..1113)
                     /locus_tag="PLG14_04155"
     CDS             complement(463..1113)
                     /locus_tag="PLG14_04155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX48357.1"
                     /translation="MIKLVMFDLDGTLADTIEDIAACVNGALARRGLPAHDIPAYKLM
                     VGNGFRTLIERAAPPEARDARLLEELRAEAQAAYDAEPVALTRPYAGVPELLSALKAR
                     GLALAVLSNKPDGLAVQVVERLFPGAGFSLVRGETPAFPRKPDPAGALDIAQRLGADP
                     SECLYVGDTDVDMETARRAGMTAVGAAWGFRGRKELEEAGADRVIAAPGELLALLD"
     gene            1238..2020
                     /locus_tag="PLG14_04160"
     CDS             1238..2020
                     /locus_tag="PLG14_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HOX48358.1"
                     /translation="MKDRDRARGGEAFLAYYGAIYQERWPALAKALSGASDSVEYDAG
                     GYEPYRLDAASVFAAESLPIPEEGEILDACAAPGGKSVVLASRMGPGLRLVANELSSE
                     RRRRLSEALDRSLPAPLRARVAVSGEDAGAMCRRNLGRFSAILLDAPCSSERHVLADA
                     QALAEWTPSRPRQLALRQWALLSSAFLMLKAGGSLVYSTCSINPAENDGVAARLAAKY
                     GESLSFDPPAHGRGERTERGLLVMPDRAGGAGPMYVCRVRKA"
     gene            complement(2115..2471)
                     /locus_tag="PLG14_04165"
     CDS             complement(2115..2471)
                     /locus_tag="PLG14_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48359.1"
                     /translation="MTFSEKMQDFINKGIAASKDLAAKAGSQAQTWGEMGVLKIEIVQ
                     LRSQAEKTTAKLGAEVYAAFAEKGQKTLSAESPAVRDLISRIGEIEKLIGEKEERYGK
                     LGGKDSDLDPEPPRQQ"
     gene            2622..3533
                     /locus_tag="PLG14_04170"
     CDS             2622..3533
                     /locus_tag="PLG14_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOX48360.1"
                     /translation="MEPFAIVLFAVLAWWALWALIALFKKAFAMFVFLLVSGAAFAFS
                     GFSLGLASGGSPFALILFVIPVLILPPMVRIVTEYERGVLFRLGRMKELLTPGLNLVF
                     PLGIDIVRKVDLRTFTIDVPKQEVITKDNVPVLVDAVVYFNVFDPKLAVTKVADYAKS
                     TSLLSQTILRSILGQHELDEMLTKRAELGVLLQKLLDDATDPWGIKVTAVEIKTVELA
                     DTMKRAMARQAEAERERRAKIIAAEGELQASEKLSQAAGVLATQPQSLQLRYLQTLTE
                     IAVEKNSTILFPLPMEFLRFLEAKGKA"
     assembly_gap    3613..3712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3892..4368
                     /gene="nrdR"
                     /locus_tag="PLG14_04175"
     CDS             3892..4368
                     /gene="nrdR"
                     /locus_tag="PLG14_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOX48361.1"
                     /translation="MRCPHCGSMDDKVIDSRTLANGESIRRRRECVSCGFRFTSYERI
                     EEKPLMVVKRDGRREPFERQKLERGVVRALEKRPVSQLSIENLVNEIEDEAAMKGKTA
                     NEIPSAELGEMLLTRLYAIDKVAYVRFASVYRKFETTDEFIQVIEGLGKVNREGRA"
     gene            4368..6470
                     /locus_tag="PLG14_04180"
     CDS             4368..6470
                     /locus_tag="PLG14_04180"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOX48362.1"
                     /translation="MADQAVFPEWKAFLGTGETGPVRYVAKRSGEVERYERSKIESAV
                     SKAFQAVGELEAASREGRVEAVAALVESKLKALIAGRHPNSIPAIEEIQDLVEEALIE
                     AGEARTAKAYILYRARREALRDSRRLMLDINTTMDGYLSQADWRVKENANVNYSLGGL
                     ILHNSGTITANYWLRNIYPEEVAEAHRNADFHLHDLSMFSGYCAGWSLRQLIAEGLGG
                     VPDKISSKPARRLSTLVQQMVNFLGVLQNEWAGAQALSSFDTYLAPFVKKDAMSEFEV
                     RQCVQSFLFGVNTPSRWGSQAPFTNVTLDWTCPPDLRDRPAVVGGEEMPFSYGDCQAE
                     MDLVNKVFIELMIAGDSEGRGFQYPIPTYNVTKDFDWDSPNARLLFEMTAKYGTPYFQ
                     NFVSSDLDPSDVRSMCCRLQLDKRELRKRGGGLFGSDELTGSVGVVTVNMPRIGYLSK
                     DRSEFYSRLDRLMDLAALSLTVKRKVITRLLDGGLFPYTKRYLGTFDNHFNTIGLVGM
                     HECAKNFLGAAYGIETEEGRAFALEVLEHMRNRLQDYQEKTGELFNLEATPAESTSYR
                     LAKHDKERWPDIIVSGEREPYYTNSSQLPVGRTDDIFEALDHQEELQARYTGGTVFHA
                     FLGEAPEDWRAVRDLVKAVASNYRVPYYTISPTFSVCPVHGYLAGEHWSCPKCKAERE
                     ESIRREIAELEHRLAAKA"
     gene            6482..7090
                     /gene="nrdD"
                     /locus_tag="PLG14_04185"
     CDS             6482..7090
                     /gene="nrdD"
                     /locus_tag="PLG14_04185"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOX48363.1"
                     /translation="MSENAAVLQRIADLKAELGRVEGTQTEVYSRIVGYYRSVRNWNA
                     GKRDEYSQRRTYGMPSGLPSATAPAPAALVKAPPEAAPAKAAPPLAAASHAASPAPAR
                     APSGAPAELFASAHPERSGMLVFTRKACPNCPPVANYVESAGLPAVFVDVDAEEGLAL
                     ARRHGVMATPTVVGLDSEGREAFRACGLGELKARLEPQAARV"
     gene            7148..7840
                     /locus_tag="PLG14_04190"
     CDS             7148..7840
                     /locus_tag="PLG14_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOX48364.1"
                     /translation="MLRSLAIRKTSLIDFPGRVAAVFFLPGCDFRCPYCHNAELVDLE
                     DPPEGLTPLEEALAFLERRKGLLGGLVLSGGEPLLHPEVARIAAAARGLGLAVKLDTN
                     GSFPGRIASVGADYVALDLKTSPGRYGLVAPDLKDSGRLVLESLGAIRASGVPFEIRI
                     TCAPGLVGPADAKAVAEVLEPGDRVYLQPFRPGGCLDPAWDGLEPLGPAEMAAILETI
                     RAAAPLASIRGA"
     gene            7901..>11751
                     /locus_tag="PLG14_04195"
     CDS             7901..>11751
                     /locus_tag="PLG14_04195"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX48365.1"
                     /translation="MSSDPLAVFHPLVAEWFREEKGEPTAVQAETWARVARGEHVLAV
                     APTGSGKTLAAFLHAISRLVSGELPAGGLSVLYVSPLKALNEDIRRNLESPLGELALR
                     FRGRGAAFPAIRVETRSGDTPEADRRRFLTRPPAILCTTPESLAILLDSPRARPILAG
                     VRLLVLDEVHAVAGNKRGSLLACSVGRLALLAGEFQRVALSATVRPPEAAADFVGGLR
                     LLPPDGSGTARRERRPVAVVAPYAEKRLSLSVEWPEAAPLGPAGLAGAEGAAAAEPDA
                     TRYAAVVPAIASRLKEAKSVIVFCDARRRAERLAFLLNEREGPGTAYAHHGSLSKEAR
                     RGVEERLKSGELKCVAATASLELGIDVGSVEEVILAGSPPEIAAALQRLGRSGHGVGE
                     ASRGVLYPFNGMDLLLAAAVAKGVEERAVEELRVPESPLDILAQVLLEMAAEGPWKID
                     ALYEAVTCFRPFEGLSRASFDSVLEMLAGRYADSRVRELEPRLYLDRATGTARAREGV
                     RALLYSSGGSIPDRGLYAMRLKGSGAKIGELDEEFVWERKAGDAFTLGSQSWRIVEIG
                     AEAVTVAPLGRGADFMPFWKGQARYRSAEASDRLLATLERLGKAGEAEGARLLAAERG
                     FSEAAAAAASRFAAAQKAAQKGAVLPGPSSVALESYADPARRGDSRCLVLYTLRGGAV
                     NEPLALALAEAWEEAFGLSPDAMADDDSVLLIVPEEGGAAGVEGSAAARLQGLIRSLA
                     EGKRLESLLRRRLEGSGVFGAQFRENAGRALLLPKGLPGKRVPLWITRLRSKRLFEAT
                     RGYADFPVTAETWRSCLADLFDLPRLRGLLEGIASGRIEVGAFETRAPSPFAREALWR
                     ETNEYLYRGDALGGKAASSLGDAAIEAALRSSRLRPRLDPDLVAGFVRKLKRLLPGWG
                     PSDPLELAEWIKERVLVPAAELPGILAAADPSCVEALRTALAEDAGLGGRVLALRLEG
                     ASEEVLVHAERLGALRSDPAALVAEWMRREGPVPADKVAALFGLPLERIEDLFEELEE
                     EGAVVLGDFGEEGGPLRAVDAENLESLLRLSRRAARPSVEARPAKDLFRLVAAVQELR
                     AGPAPSVPAGAGPEDARSALERALDRLAGYSGPASLWESEFLPARVPGYSPSLLDAAL
                     SAEPRLWFGTGRERLAFARAEELELFAKGGASRLVPPDSPPLDFWELKERAGLSSKEL
                     ALALWAEAWKGLAGAEGFEAVRKGLAVRFGRDLPDAVPAEAAPEPFGARRRVPRALRE
                     RWKAGAPVAGRWFALDLGPDEG"
BASE COUNT         1614 a         4335 c         3968 g         1734 t
ORIGIN      
        1 taggccttga cgccgttggc cgcgaagacg cgggcggtca tctcggcgaa gacgtccgag
       61 tagcggcggg aatcgtaggc gacgcaggcc gacaggctct tcccggggaa ggtcttcctg
      121 aggtagtcgc agaggccctg ggtggccctg gtgacgacga ggctgttcat ccggttgtag
      181 ccgccgccga tgacgccgcg gaggccgccc gtgccgaact cgaggtcgcg gtagaaccgg
      241 tcctcgagct ccttccagtc gccggcggcg aggagcttct ccacctcggc cttgaaggcg
      301 gcgtcggtct cccgggcgac gtagtcgcgg gcccgttctt cgatcttctc gcgttccatg
      361 ctcaactcct tggcgtcgtg gatgcctgtc ctacctattc taggccggga aggcgcggct
      421 gggaaagtgt ctccggaccg ggaagcgggt cttccgggtc tttcaatcga gaagggcgag
      481 gagctctccc ggcgcggcga tgaccctgtc ggcgccggcc tcctcgagct ccttgcggcc
      541 ccggaagccc caggccgccc cgacggccgt catgccggcg cgcctggccg tctccatgtc
      601 gacgtccgtg tcgccgacgt agaggcactc cgaggggtcc gcgccgagcc tttgcgcgat
      661 gtccagggct cccgcggggt cgggcttccg ggggaaggcg ggggtctccc cgcggaccag
      721 ggagaagccc gctccgggga agagccgctc gacgacctgg acggccaggc cgtcgggctt
      781 gttggagagg acggccaggg ccagtccgcg ggccttgagg gcggacagga gctcgggcac
      841 gcccgcgtag ggccgcgtga gggccacggg ctcggcgtcg taggcggcct gggcctcggc
      901 cctgagctcc tcgaggaggc gggcgtcgcg ggcctccggg ggagccgcgc gctcgatgag
      961 ggtcctgaag ccgttcccca ccatgagctt gtaggcggga atgtcgtggg cggggaggcc
     1021 gcggcgggcc agggcgccgt tcacgcaggc cgcgatgtct tctatcgtgt cggccagggt
     1081 gccgtcgagg tcgaacatga cgagcttgat catgcttttc tttcctgggc gcttcaccga
     1141 gggccgcgcc acggggtagg ataggcccga ggcgcgaccg ggcgccgccc caaggcccgg
     1201 ccagtctagc gggcaatctc aaggagcgtc aattcccgtg aaggatcgag accgcgcgag
     1261 gggaggggaa gccttcctcg cctactacgg agccatctac caagagcgct ggccggccct
     1321 ggccaaggcc ctctcgggcg cgtcggattc ggtggagtac gacgcgggag gctacgagcc
     1381 ctacaggctg gacgcggcct ccgtcttcgc ggcggaatcc ctccccatcc cggaggaggg
     1441 cgagatcctg gacgcctgcg ccgcccccgg cggcaagagc gtcgtcctcg cgagccgcat
     1501 ggggccgggc ctccgcctgg tcgccaacga gctctcctcg gagcggcgcc gccgcctctc
     1561 ggaggccttg gaccggagcc tgcccgcgcc tctgcgggcc cgggtggccg tctccggcga
     1621 ggacgcggga gccatgtgcc gtcggaacct gggccgcttc tcggccatcc tcctcgacgc
     1681 gccctgctcc agcgagcggc acgtcctcgc cgacgcccag gccctggcgg agtggacgcc
     1741 ttcgcggccc aggcagctcg ccctgaggca gtgggccctc ctctcctcgg ccttcctcat
     1801 gctcaaggcg ggcggcagcc tcgtctactc gacctgctcc ataaacccgg cggagaacga
     1861 cggcgtcgcc gcccgcctgg ccgcgaaata cggcgagtcc ttgagcttcg acccgcccgc
     1921 ccatggccgc ggcgagcgga cggagcgcgg cctcctcgtg atgcccgacc gcgcgggcgg
     1981 ggccgggccg atgtacgtct gccgggtccg caaggcctag cgcgcccgcg ggcgcacgcc
     2041 acgccgccgg cgccatgctt gcgggcgcgc ggcccgcgac gtggagcccg aggggccgcc
     2101 ctgccttccg cggcctactg ctggcgcggc ggctcgggat ccaggtcgga gtccttgccg
     2161 ccgagcttgc cgtaccgctc ctccttctcg ccgatgagct tctctatctc gccgatccgc
     2221 gagatgaggt cgcggacggc cggagactcg gcggagaggg tcttctgccc cttctcggcg
     2281 aaggcggcgt agacctcggc cccgagcttg gccgtggtct tctcggcctg gctccggagc
     2341 tggacgatct ctatcttgag gacgcccatc tcgccccagg tctgggcctg gctcccggcc
     2401 ttcgccgcga gatccttcga cgccgcgatg cccttgttga tgaaatcctg catcttctcg
     2461 gaaaaggtca tgtgagcctc cgtccctgaa tggtagcgcg ttccgggcgg atcggccagc
     2521 ccggccgccg cgcgccggag ccgcccggtc gagacctcgg ccggcgcgag gcgccttgcc
     2581 tcgggccccg gatgcgtcta gaattcgagc cggaggcaac catggaacct ttcgccatcg
     2641 tgctcttcgc cgtcctggcc tggtgggcgc tctgggccct catagccctc ttcaagaagg
     2701 ccttcgcgat gttcgtcttc ctcctcgtct cgggcgcagc cttcgcgttc agcggcttct
     2761 ccctcggcct cgcctcgggc ggctcgccct tcgccctcat cctcttcgtc atccccgtcc
     2821 tcatcctgcc tccgatggtc aggatcgtga cggaatatga gcggggcgtc cttttccggc
     2881 tcggaaggat gaaggaactc ctgacgccgg ggctcaacct cgtcttcccc ctgggcatcg
     2941 acatcgtgcg caaggtcgac ctgcgcacct tcacgatcga cgtcccgaag caggaggtga
     3001 tcacgaagga caacgtcccc gtcctcgtgg acgccgtcgt ctacttcaac gtcttcgacc
     3061 cgaagctcgc cgtcacgaag gtcgccgact acgccaagtc gacgagcctc ctctcccaga
     3121 ccatcctccg ctccatcctc ggccagcacg agctggacga gatgctcacg aagcgggccg
     3181 agctcggcgt cctcctccag aagctcctgg acgacgctac cgacccctgg ggcatcaagg
     3241 tgacggccgt ggagatcaag acggtcgagc tcgccgacac gatgaagcgg gccatggccc
     3301 gccaggccga ggccgagcgc gagcgccgcg cgaagatcat cgcggcggag ggcgagctcc
     3361 aggcgagcga gaagctttcc caggccgccg gcgtcctcgc gacccagccc cagtccctcc
     3421 agctgcgcta cctccagacc ctcaccgaga tagcggtgga gaagaactcg acgatcctct
     3481 tccccttgcc catggagttc ctgcgcttcc tggaggcgaa gggaaaggcc tgaaggccgg
     3541 gggccttcca gccggtccgc cggggcgcgc gggcctacgg cggctttccc caaagccgcc
     3601 gcaggccgac cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgtggc
     3721 gcagctgtgt taaccgtcag cttggcctga gtcaagcggt caaagaaaaa tttactctcc
     3781 cggaaagcct tgacaggggg cggtgtctac cctatatgct gtagctggtt tcaagaacta
     3841 ccctacccct ggtagggata ttggccaaat tggatacatg acaggaacac gatgcgctgc
     3901 ccccactgcg gaagcatgga cgacaaggta atcgactcca ggaccctcgc gaacggcgag
     3961 agcatcaggc gaaggcgcga gtgcgtctcc tgcggcttcc gcttcacgag ctacgagagg
     4021 atcgaggaaa agcccctcat ggtcgtcaaa cgcgacggcc ggcgcgagcc cttcgagcgc
     4081 cagaagctcg agcggggcgt ggtgcgggcc ctcgaaaagc ggcccgtctc ccagctttcc
     4141 atagagaacc tcgtcaacga gatcgaggac gaggccgcca tgaagggcaa gaccgcgaac
     4201 gagatcccga gcgccgagct cggcgagatg ctcctcaccc ggctctacgc catcgacaag
     4261 gtcgcctacg tgcgcttcgc ctccgtgtac cgcaaattcg agactaccga cgagttcata
     4321 caagtgatcg agggcctggg caaggtcaat cgcgagggga gggcatgatg gcggaccagg
     4381 cggtgtttcc cgagtggaag gcgtttctcg ggacgggcga gacgggcccc gtccgctacg
     4441 tcgccaagcg ctcgggcgag gtcgagcgct acgagcgctc caagatcgag tcggccgtct
     4501 ccaaggcctt ccaggccgtg ggcgagctcg aggccgcctc ccgcgagggg cgggtcgagg
     4561 cggtcgccgc cctggtcgag tcgaagctca aggccctcat cgcggggcgc caccccaact
     4621 cgatccccgc gattgaggag atccaggacc tcgtggagga ggccctcatc gaggccggcg
     4681 aggccaggac ggcgaaggcc tacatcctct accgcgccag gcgcgaggcc ctgcgcgact
     4741 cccgcaggct catgctcgac atcaacacga cgatggacgg ctacctgtcc caggccgact
     4801 ggcgggtcaa ggagaacgcc aacgtcaact actccctggg cggcctcatc ctccacaact
     4861 cgggcacgat cacggccaac tactggctcc ggaacatcta ccccgaggag gtcgcggagg
     4921 cgcaccggaa cgcggacttc cacctccacg acctctccat gttctcgggc tactgcgccg
     4981 gttggtccct gcgccaactc atcgccgagg gcctgggcgg cgtccccgac aagatcagct
     5041 ccaagccggc gcgccgcctc tccaccctcg tccagcagat ggtcaacttc ctgggcgtcc
     5101 tgcagaacga gtgggcgggc gcgcaggccc tctcctcctt cgacacctac ctcgcgccct
     5161 tcgtgaagaa ggacgcgatg agcgagttcg aggtcaggca gtgcgtccag tccttcctct
     5221 tcggcgtcaa cacgccgagc cgctggggca gccaggcccc cttcaccaac gtgaccctgg
     5281 actggacctg cccgcccgac ctccgggacc ggcccgccgt cgtgggcggc gaggagatgc
     5341 ccttcagcta cggcgactgc caggcggaga tggacctggt caacaaggtc ttcatcgagc
     5401 tcatgatcgc gggcgactcc gagggccgcg gcttccagta ccccatcccc acctacaacg
     5461 tcacgaagga cttcgactgg gattcgccca acgcgcgcct cctcttcgag atgaccgcca
     5521 agtacggcac cccctacttc cagaatttcg tgagctccga cctggacccg agcgacgtgc
     5581 gctccatgtg ctgccgcctg cagctcgaca agcgcgagct ccgcaagcgg ggcggcggcc
     5641 tcttcggctc cgacgagctc acgggctcgg tcggcgtcgt gaccgtcaac atgcccagga
     5701 tcggctacct ttccaaggac cgctcggagt tctactcccg cctggaccgc ctcatggacc
     5761 tggcggccct atccctcacc gtgaagcgga aggtcatcac ccgcctcctg gacggcggcc
     5821 tcttccccta cacgaagcgc tacctcggga ccttcgacaa ccacttcaac accatcggcc
     5881 tcgtcggcat gcacgagtgc gcgaagaatt tcctgggcgc ggcctacggc atcgagacgg
     5941 aggagggcag ggccttcgcc ctcgaggtcc tggagcacat gcggaaccgc ctccaggact
     6001 accaggagaa gacgggcgag ctcttcaacc tggaggcgac ccccgcggag tccacctcct
     6061 accgcctcgc gaagcacgac aaggagcgct ggcccgacat catcgtctcg ggcgagcgcg
     6121 agccctacta cacgaactcg agccagctcc ccgtcggccg cacggacgac atcttcgagg
     6181 ccctggacca ccaggaggag cttcaggccc ggtacacggg cggcaccgtc ttccacgcct
     6241 tcctgggcga ggcgcccgag gactggcgcg cggtgcgcga cctcgtcaag gcggtggcct
     6301 cgaactaccg cgtgccctac tacacgatat cgccgacctt ctcggtctgc cccgtgcacg
     6361 gctacctggc cggagagcac tggagctgcc cgaagtgcaa ggccgagagg gaagagtcga
     6421 tccgccggga gatagccgag ctcgagcaca ggctcgcggc gaaggcgtga aggagaagag
     6481 catgagcgag aacgccgccg tactgcagcg cattgccgac ctcaaggccg agcttggccg
     6541 ggtcgaaggc acccagaccg aggtctacag ccgcatcgtc ggctactacc gttccgtccg
     6601 caactggaac gcgggcaagc gcgacgagta ctcgcagcgc cggacctacg gcatgccgag
     6661 cggcctgccc tccgccaccg cccccgcgcc cgcggccctt gtgaaggctc cgcccgaggc
     6721 cgcgccggcc aaggccgcgc cccccctcgc ggcggcctcc catgccgcgt cgcccgctcc
     6781 cgcccgcgcg ccctccgggg cgcccgcgga gctcttcgcc tcggcccacc ccgagcgcag
     6841 cggcatgctc gtcttcaccc ggaaggcctg ccccaactgc ccgcccgtcg ccaactacgt
     6901 ggagagcgcg ggcctgcccg ccgtcttcgt cgacgtcgac gccgaggagg gcctcgccct
     6961 cgcgaggcgc cacggcgtga tggcgacccc gaccgtggtc ggcctcgatt ccgagggccg
     7021 cgaggccttc cgggcctgcg gcctgggaga gctcaaggcc cgcctggagc cccaggcggc
     7081 gcgggtctag cctcaaggcc caggcgcccc ggctcttgtc cggccggggc gccgcctcca
     7141 tccctccatg ctccgatcgc tggccatccg caagacgagc ctcatcgatt ttcccggccg
     7201 cgtcgccgcg gtcttcttcc tccccggctg cgacttccgc tgcccctact gccacaacgc
     7261 cgagctcgtc gaccttgagg atccgccgga gggcctgacg ccgctggagg aggccctcgc
     7321 cttccttgag cggaggaagg ggctcctggg cggccttgtc ctctcaggcg gagagcccct
     7381 cctccatccg gaggtcgccc ggatcgcggc cgcggcgcgc gggctcgggc tggcggtgaa
     7441 gctcgacacg aacggctcct tccccgggcg catcgcctcg gtcggcgcgg actacgtggc
     7501 cctcgacctc aagacctccc ccggccgcta cggcctcgtc gcgccggacc tcaaggactc
     7561 gggcaggctc gtcctcgagt ccctgggggc gatccgcgcc tcgggcgtcc ccttcgagat
     7621 ccgcataacc tgcgcgccgg gcctcgtcgg gcccgcggac gccaaggccg tcgcggaggt
     7681 cctggagccc ggggacaggg tctacctcca gcccttccgt ccgggcggct gcctcgatcc
     7741 cgcctgggac ggactcgagc ccctgggccc ggccgagatg gccgcgatcc tggagacgat
     7801 cagggcggcc gctccgcttg cttcgatccg cggcgcctaa aggccgcgcc tgagccgcgc
     7861 cgccttgcgg gagggcctga ggcggcgcta cagtgcctcc atgagctccg atcccctggc
     7921 cgtcttccac cccctggtcg ccgaatggtt ccgcgaggag aagggcgagc cgacagccgt
     7981 ccaggcggag acctgggccc gcgtggcccg cggggagcac gtgctcgcgg tggcgccgac
     8041 gggttcgggc aagaccctcg cggctttcct gcacgcgatc tcccgcctcg tctcggggga
     8101 gcttcccgcc ggcggcctct ccgtgctcta cgtctccccc ctcaaggccc tcaacgagga
     8161 catcaggcgg aacctcgagt cgccgctcgg cgagctcgcc ctgcgcttcc gcgggcgcgg
     8221 cgccgccttc cccgcgatcc gggtggagac gaggtcgggc gacacgcccg aggcagaccg
     8281 gcgccgcttc ctgacccgcc cccccgccat cctctgcacc accccggaga gcctcgccat
     8341 tctcctcgac tcgccccgcg cccgacccat cctcgcgggc gtgaggctcc tcgtcctgga
     8401 cgaggtgcac gcggtggccg gcaacaagcg cggctccctc ctcgcctgct cggtcggccg
     8461 cctggccctc ctcgcggggg agttccagcg cgtggccctc tccgcgaccg tccgcccccc
     8521 cgaggccgcg gccgacttcg tgggaggcct ccgcctcctt cctccggacg gctccgggac
     8581 ggcgaggcgc gagcggcggc ctgtcgccgt cgtggccccc tatgctgaga aacgactgtc
     8641 cctctcggtc gagtggcccg aggcggcgcc cctcggcccc gccggcctcg cgggcgcgga
     8701 aggcgcggcc gcggccgagc ccgacgcgac gcgctacgcg gccgtcgtgc ccgccatcgc
     8761 ctcaaggctc aaggaggcca agagcgtcat cgtcttctgc gacgcgaggc gccgcgccga
     8821 gcgcctggcc ttcctcctca acgagcgcga ggggccgggc accgcctacg cgcaccacgg
     8881 ctccctctcc aaggaggcgc ggcgcggcgt ggaggagcgg ctcaagtcgg gcgagctcaa
     8941 gtgcgtcgcc gcgacggcct ccctggagct cggcatcgac gtgggctcgg tggaggaggt
     9001 gatcctggcc ggctcgcccc cggagatcgc cgcggccctc cagcgcctgg gccgttcggg
     9061 ccacggcgtc ggggaggcct cccgcggcgt cctctacccc ttcaacggga tggacctcct
     9121 cctcgccgcg gcggtggcga aaggcgtgga ggagcgggcc gtcgaggagc tccgcgtgcc
     9181 ggagtcgccg ctcgacatcc tggcccaggt cctcctggag atggcggcgg aggggccctg
     9241 gaagatcgac gcgttgtacg aagccgtcac ctgcttcagg cccttcgagg ggctctcccg
     9301 cgcctccttc gactcggtcc tggagatgct cgcgggccgc tacgccgact cgcgcgtgcg
     9361 cgagctcgag ccgcgcctct acctggaccg ggcgaccggc acggccaggg ccagggaggg
     9421 agtccgggcc ctcctctact cctcgggagg ctcgattccc gatcgtggcc tctacgcgat
     9481 gcgcctcaag ggcagcggcg ccaagatcgg cgagctcgac gaggagttcg tctgggagcg
     9541 caaggccggc gacgccttca ccctgggctc ccagtcctgg cgcatcgtgg agatcggggc
     9601 cgaggcggtg acggtcgcac cccttgggcg cggcgcggac ttcatgccct tctggaaggg
     9661 tcaggcccgc taccgcagcg ccgaggcctc ggaccggctg ctcgcgacgc ttgagcggct
     9721 cgggaaggcg ggcgaggccg agggggcgcg cctcctcgcg gccgagcgcg gcttctccga
     9781 ggcggcggcg gcggcggcct cgcgcttcgc cgcggcgcag aaggcggccc agaagggcgc
     9841 ggtcctgccc ggcccctcga gcgtcgccct ggagtcctac gccgaccccg cgcgccgcgg
     9901 cgactcccgc tgcctcgtgc tctacaccct caggggaggc gcggtcaacg agcccctggc
     9961 cctggccctc gccgaggcct gggaagaggc cttcggcctt tcccccgacg cgatggccga
    10021 cgacgattcc gtcctcctca tcgtacccga ggaggggggc gccgccggcg tggaggggag
    10081 cgcggcggcc cgcctccaag ggctaatccg gagcctcgcg gagggaaagc gcctcgagtc
    10141 cctcctccgg cgccgcctcg agggctcggg cgtcttcggc gcccagttcc gggagaacgc
    10201 gggccgcgcc ctcctcctgc ccaagggcct gccggggaag cgcgtgccgc tctggatcac
    10261 gaggctccgc tccaagcggc tcttcgaggc gacgaggggc tacgccgact tcccggtgac
    10321 ggcggagacc tggcggagct gcctggccga cctcttcgac cttccccggc tccgcgggct
    10381 cctggagggc atcgcgtcgg ggcgcataga ggtgggagcc ttcgagacgc gggctccctc
    10441 gcccttcgcc cgcgaggccc tctggcgcga gacgaacgag tatctctatc ggggcgacgc
    10501 gctgggcggc aaggccgcct cctcgcttgg cgacgcggcc atcgaagcgg ccctgcgttc
    10561 ctcgcgcctc cggccccgcc tggacccgga tctcgtcgcg ggcttcgtgc ggaagctcaa
    10621 gcgcctcctt ccgggctggg ggccttccga tcccctcgag ctcgccgaat ggatcaagga
    10681 acgcgtcctc gtccccgccg ccgagctccc cggcatcctc gccgcggccg atccctcctg
    10741 cgtcgaggcc ctgcggacgg ccctggccga ggacgccggc ctgggcggcc gcgtccttgc
    10801 cctgcgcctc gagggcgcct cggaggaagt cctcgtccac gccgagcggc tcggagccct
    10861 gcgctccgac cccgcggccc tcgtcgccga atggatgcgc cgcgagggcc ccgtgccggc
    10921 cgacaaggtg gccgcgctct tcggccttcc cctagagcgg atcgaggatc tcttcgagga
    10981 gctcgaggag gagggcgccg tcgtcctcgg agacttcggc gaggagggcg gcccgcttcg
    11041 cgccgtcgac gccgagaacc tcgagtccct cttgcggctg tcgcgcaggg cggcccggcc
    11101 ctcggtggag gcaaggccgg cgaaggacct cttccgcctc gtcgccgcgg tccaggagct
    11161 ccgcgccggg ccggcaccct ccgtccccgc gggcgccggg cccgaggacg cgcgctccgc
    11221 gcttgagagg gccctcgaca ggcttgcggg ctactccggc ccggcttcgc tctgggagtc
    11281 ggagttcctg ccggcgaggg tgccgggcta ttccccgtcc ctgctcgacg ccgctctctc
    11341 cgcggagccg cggctctggt tcgggaccgg ccgcgagcgg ctcgccttcg cccgggccga
    11401 ggagctcgag ctcttcgcga agggcggcgc ctcccgcctc gtccctcccg attcgccgcc
    11461 cctcgatttc tgggagctca aggagcgggc cggcctctcc tccaaggaac tcgccctcgc
    11521 gctctgggcg gaggcctgga agggcctcgc gggcgcggag ggcttcgagg cggtgaggaa
    11581 gggcctggcc gtccgcttcg gccgcgacct gccggacgcc gtcccggccg aggcggctcc
    11641 cgagcccttc ggcgcgaggc gccgcgtgcc gagggccctc cgcgagcgct ggaaggccgg
    11701 cgctccggtc gcgggccggt ggttcgccct cgacctgggc cccgacgagg g
//
LOCUS       DAOOSJ010000054        11668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3654_length_11668_cov_5.885818, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000054 DAOOSJ010000000
VERSION     DAOOSJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_11668_cov_5.885818"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLG14_04200"
     CDS             complement(<1..1321)
                     /locus_tag="PLG14_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48366.1"
                     /translation="MITVTDLELSFGERTLFKDVNLKFTPGNCYGIIGANGAGKSTFL
                     KLLSGELRQDKGEIVIGSGKRMAVLAQDHFAFEENRVLDTVIMGWQKLFQVKLEREAL
                     YEKADFTEADGIRASELEGEFAELGGWEAEAQAAILLSGLGVPEAHHEKLMKEVDEGV
                     KVRVLLAQALFGNPDILLLDEPTNGLDLESIDWLEDFLIEFPNIVIVVSHDRHFLNAV
                     CTHTCDIDFGKVRMYSGNYDFWFKMNRILVQQQKDLKKRNEDKAKDLKDFIQRFSANA
                     AKSKQATSRKKILEKLELDAIVPSSRRFPYVGFKPDRDVGNNVLKVVKLEKSFEGEKV
                     LSGYDLTVNRGDKIAFVGREHLAKTMLFEIAAGEQAADSGDVYWGQTISFSYFPKDNT
                     RLFDSDLSITDWLKQYANSDDDTYIRSFLGRMLFSGDEALKPVRVLSG"
     gene            1433..2596
                     /locus_tag="PLG14_04205"
     CDS             1433..2596
                     /locus_tag="PLG14_04205"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX48367.1"
                     /translation="MSEHGRLPLRKYNAPEILFGEGAARKAGSYALRFGASKVLLVTD
                     PGVRAAGIVGPIEDSLKEAGLGCVVFDGVSPNPRDEEARAGLELYRERNCDLIVAVGG
                     GSPMDCAKGIGLSAANGRDVLEFEGIDAVPEPGPPLLCVPTTAGTSADVSQFAIINDT
                     KRHVKIAIVSKKAIPDAALVDPLATVTMPPELTAATGMDALVHAMEAYVSTAASALTD
                     IDALEAARLVAANLPGAVADGGNLERRSGMTLASLLAGRAFSNASLGVVHAMAHVLGG
                     ALDLPHGVCNALLLEAAVAANYQSAAARYDEFAAVLARGLGEEAGTGLPSLLGLIRRL
                     KSLVGLDYGLARLGLKTEDIPRLAGFALKDACLVTNPRVFGLAELEAIYAGAL"
     gene            2580..3932
                     /locus_tag="PLG14_04210"
     CDS             2580..3932
                     /locus_tag="PLG14_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48368.1"
                     /translation="MPERSSRAAPGGTTSAPGGGANGGAGRTEGDLSRGEAQAGGGRE
                     RLRDRFMGIDESFSVKSYYPQLKERLRELEAERTRALESEARLAKLNTELESLVESRT
                     AALRSAMSGLTEANAELERTLSDLRAAQDRLVLSEKMAAVGRLVAGIAHDVNSPLGAL
                     LSSVSSIDEGLVGYLESFPSFYASLGKGDFGRYLGLVAAGAEKARSLESGRDRARKDR
                     LAELLAPECPDALSVAEAAEELGVFDEAEAAVGEIGFRRAARIIAEAAKTVALIRSAA
                     VAAEATRRTARIIEALRRFSLGSEAKSSESIALREEFAGVLSLFQDKFKRELAVDLDM
                     DEEIRVSGDREALGQVWIGLVANALQAMEYRGRLGLSAREEGGSVLVSVSDTGRGIRP
                     EDRDRVFEPFFTTKKLGEGMGLSLSIARKVVEANGGEIDFESEPGRTVFRVRLKAARK
                     "
     gene            4060..6150
                     /locus_tag="PLG14_04215"
     CDS             4060..6150
                     /locus_tag="PLG14_04215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOX48369.1"
                     /translation="MQSDNSSSSGASAAPSALGGAATPPPAWDLSPEYASFDDPAFAS
                     DLARVEALAAELEALAPLAAAALARGAGLEALDPASEPGLVAAVLSSFAIGVEIDERF
                     GNLRVYANCVASVDGGDARAKEYRGRFGSLGARIEKADSAFALLLARAPEAFVKAVLA
                     APEAAGQAFRVSQARKYRDRLLELAEEKTIAALAPDGRDAWGRLYDSLSGTAKALYRG
                     PSPEGGADASAPREIGLSEAASLLRDPDRRVREAAFRAQTEAFRAHEESLAAILNSIA
                     GFRLAEYGMRSRKAPMHFLDAALASNRLERATLDAMLGVVAEERELGRRALRLQARLM
                     GVPRLACYDTLAPAPRLPGGEGRAYSFDEGLELVRGAYASVDPAMGDFVLEMRDARRI
                     EARVLPAKRPGAYCTRFPKTRTPRVFLTYRGALSDVSTLAHELGHAYHGKLLASLPLA
                     ETEYPMNLAETASTFAETALGDYLDSLGQGAGAAGDRAAGGAAGPGGGAAADGPGSGA
                     AGGAGAAEGQAAGGAAGLSQAELAGLLEVAWSDAQDAATFLVNIPARFAFEKAFYERR
                     SEKPLGPAALSALMDETMREHYGDALSEYDAGFWRSKLHFYMSGLSFYNFPYTFGYLF
                     SLGVYSRRAELGKGFHEAYCALLRDTGRMETEALAERHLGVDLRKPDFWRASVDIVRR
                     KVDRFQALVERLAP"
     gene            complement(6147..7259)
                     /locus_tag="PLG14_04220"
     CDS             complement(6147..7259)
                     /locus_tag="PLG14_04220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOX48370.1"
                     /translation="MGAVSYTILVVDDSATNRSYLKYILEEDGYRVVQAANGEACLKS
                     AGAEKPILILLDVVMPGIDGFETLRRLKEEPGSARTPVIMLTSLEDQDSKLKAFELGA
                     VDYIVKSANPMEVRARVRVHVRLALANEELVAARRESIMQIAQAQRSLLVRPQELPKA
                     RFSVYYRSLHEAGGDFYDVVPVAEDVFLYLMADVAGHDLGTSYVTPAVKVLLKQFSTP
                     AYSLEETVGLMNGVLSQTVCADTYLTAYALRLNRRSRKAVYMAAGHPSALYLPARGEA
                     RFLECENPMIGMIEESTYRSESMEVEEGDRFVLFTDGLVECGPSPEAWKRSRGGLLDV
                     VGGLRGAPLAKLPEALVLAMGADRAKDDVAVLAVEV"
     gene            7413..7994
                     /locus_tag="PLG14_04225"
     CDS             7413..7994
                     /locus_tag="PLG14_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX48371.1"
                     /translation="MAEEELEDQVLVHRSLAGDEVAFRRLVERYADRVLRFCEARLGS
                     GEDAADAAQEVFIRVYRSLGSYRLGASFPAWLFAIAANRVRTRWTRAATERKKIERSA
                     AEAEGASSPDPEDEALRSLQGEAVRSAVAALPKEQRDCVELYYFGGLGVAECAEALGL
                     GAEAVKSRLFRARRTLRAALETGQPKPPSEGIL"
     gene            7997..8686
                     /locus_tag="PLG14_04230"
     CDS             7997..8686
                     /locus_tag="PLG14_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOX48372.1"
                     /translation="MDCKEVEGLLRSWEEGRNPPEAALKELIGHIVSCASCSAARGGI
                     LPLLRLELGREAREEAGPTAPSAAARSLADGVMARLSSLEAKAPASGGRRPTRPVLVL
                     QADMPRRKRVAWPLLATAAAALLVAGLGFGLHFFGPGDGGVVTVRFVLDAPEAKSVYL
                     AGNFSDWKGESLPLERKTPGEAWELTLKLRKDNMYVYNFIVDGERWIVDPSVPETVED
                     GFGGSSSLLRL"
     gene            8695..9246
                     /locus_tag="PLG14_04235"
     CDS             8695..9246
                     /locus_tag="PLG14_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48373.1"
                     /translation="MKAFIGAKRLMYAAAAAAFVLAAAGAASAAESAPAAGAAGAAFE
                     TGAASAPGSGDQSSDEAWDEAFALAYAAALAGAPFEPSALALRIVEAETSALRRDWPP
                     EAAASRVLELSLCAESALRFGSSPQETRALIRARLRFDGPGPSASRFSKELGRSLKAS
                     ASGGAKGAAAPGGFGGFGGRGRR"
     gene            9243..9959
                     /locus_tag="PLG14_04240"
     CDS             9243..9959
                     /locus_tag="PLG14_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48374.1"
                     /translation="MSLLRARLLLVPLLLAAASLAAGQGTRDWFERDPEAAAYAGIRS
                     GVEALSLRAQGEGLPESLLLRRLVEGAAKRAEPERLLLALSEETERLVALKKALGGRG
                     LLPRDPAKAEGILAEGSVILRAGVGLPLLLSAVDASQSGGAGPDASAARAYAALGAAI
                     SVNARFPLGEAAFRALALALAGSSLPEGRFGAFVAVFARGRASGLAADRIAEVSVAVL
                     DAGGSLDRVEKELERRMRKP"
     gene            9956..10879
                     /locus_tag="PLG14_04245"
     CDS             9956..10879
                     /locus_tag="PLG14_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48375.1"
                     /translation="MTRLAARRFAVLTALLSLAASPSLAEAVLGAGMVYDEASGPSLA
                     ASAQGAVETIGSRLALRLEGEGEASVALPAPLGAAPFDPSASGAEAFGLVELSYTAGS
                     LNCLGAASGGLSLEEGAVSYSAGLRGRVAVQALELSASLSPRLGFDSSAQGGLELGLL
                     GECSFLAADLVLKPRLDLSAFDFRDGSRAFRAMPGLALSWYPLVPLSISFEAGYRREW
                     TAEGAATSGIPLSLSLFASPLDKVFLRAGAEALAPLPSGALSDAAAYLELSFALAGRA
                     GRELRLPLRLSYDGDAEARYSLYAGLELSFD"
     gene            11017..11556
                     /locus_tag="PLG14_04250"
     CDS             11017..11556
                     /locus_tag="PLG14_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48376.1"
                     /translation="MKRLATLVLLAVIVLGAGAQTIGLEEPALEAAIREMNGIPQEQL
                     AAYYADLALRYRFKAKDIERLALRYRLEAGEVCLVAFMARTRSMTMERVAKMKTPDQT
                     WGEVATRLRLNQGSPEFGKLKEDAEGDLLRVRDRERLRLETQTQLQTQTQLQTQTQTQ
                     LKTQDGEGSGSGAGTGKGK"
BASE COUNT         1641 a         4171 c         4091 g         1765 t
ORIGIN      
        1 ctcccgagag gacgcgcaca ggcttcaggg cctcgtcccc ggagaagagc atccggccca
       61 ggaaggagcg gatgtaggtg tcgtcgtcgg agttcgcgta ctgcttgagc cagtccgtga
      121 tggagaggtc cgagtcgaag agcctcgtgt tgtccttggg gaagtaggag aaggagatgg
      181 tctggcccca gtagacgtcg ccggagtcgg cggcctgctc gcccgcggct atctcgaaga
      241 gcatggtctt cgcgaggtgc tcgcggccga cgaaggcgat cttgtcgccg cggttcaccg
      301 tgaggtcgta gccggagagg accttctcgc cctcgaagct cttctcgagc ttgacgacct
      361 tgaggacgtt gttgccgacg tcccggtcgg gcttgaagcc gacgtagggg aagcgccggg
      421 aggagggcac gatcgcgtcg agctcgagct tctccaggat cttcttgcgg gaggtggcct
      481 gcttgctctt ggccgcgttc gcggagaagc gctggatgaa gtccttgagg tccttcgcct
      541 tgtcctcgtt ccgcttcttg aggtccttct gctgctggac gaggatgcgg ttcatcttga
      601 accagaagtc gtagttgccc gagtacatcc tcaccttgcc gaagtcgatg tcgcaggtgt
      661 gggtgcagac ggcgttgagg aagtgccggt cgtgggagac gacgatgacg atgttcggga
      721 actcgatgag gaagtcctcc agccagtcga tggactcgag gtccaggccg ttggtcggct
      781 cgtcgaggag gaggatgtcg gggttgccga agagggcctg ggccaggagg acgcggacct
      841 tcacgccctc gtcgacctcc ttcatcagct tctcgtggtg ggcctcgggc acgcccaggc
      901 cggagaggag gatggcggcc tgcgcctcgg cctcccagcc gcccagctcg gcgaattcgc
      961 cctcgagctc gctggcgcgg atgccgtcgg cctccgtgaa gtcggccttc tcgtagaggg
     1021 cctcgcgctc cagcttcacc tggaagagct tctgccagcc catgatgacg gtgtcgagga
     1081 cgcggttctc ctcgaaggcg aagtggtcct gggccaggac ggccatgcgc ttgcccgagc
     1141 cgatgacgat ctcgcccttg tcctgcctga gctcgcccga gaggagcttg aggaaggtgg
     1201 acttgcccgc gccgttggcg ccgatgatgc cgtagcagtt gccgggggtg aacttgaggt
     1261 tcacgtcctt gaagagagtc cgctccccga aggagagctc gaggtctgtt actgtgatca
     1321 cccctcattt ttcgcagaaa accgcaggat attcaagggg gtggcatccg gcgcggccgc
     1381 ttcggggcgg acgaatttgc gaaatgcccc gagatcggcc atagttggga ccatgagcga
     1441 acatggccgc ctgcccctgc gcaagtacaa cgcgccggag atcctcttcg gcgaaggtgc
     1501 cgccaggaag gcggggagct acgccctgcg cttcggcgcc tcgaaggtcc tcctcgtcac
     1561 cgacccgggc gtccgcgcgg cgggcatcgt cgggccgata gaggattccc tcaaggaggc
     1621 gggcctgggc tgcgtggtct tcgacggagt gagcccgaac ccccgcgacg aggaggcgag
     1681 ggcgggcctc gagctctacc gggagcggaa ctgcgacctc atcgtcgcgg tcggcggcgg
     1741 cagccccatg gactgcgcca agggcatcgg cctctccgcg gcgaacggcc gggacgtcct
     1801 ggagttcgag ggcatcgacg cggtgcccga gccggggccg cccctcctct gcgtcccgac
     1861 gaccgcgggc acgagcgccg acgtctccca gttcgccatc atcaacgaca cgaagcgcca
     1921 cgtgaagatc gccatcgtct ccaagaaggc catacccgac gcggccctcg tcgatcccct
     1981 cgccaccgtc acgatgcccc ccgagctcac cgcggcgacg ggcatggacg ccctggtcca
     2041 cgcgatggag gcctacgtct ccaccgccgc ctcggccctc accgacatcg acgcattgga
     2101 ggcggcgagg ctcgtggccg cgaacctccc cggcgcggtg gccgacggcg ggaacctgga
     2161 gaggcggagc gggatgaccc tcgcgagcct cctcgcgggc agggccttct ccaacgcgag
     2221 cctgggcgtc gtccacgcga tggcccacgt cctgggcggc gccctcgacc ttccccacgg
     2281 cgtctgcaac gccctcctcc tcgaggccgc cgtcgccgcc aactaccagt ccgccgccgc
     2341 ccgctacgac gagttcgccg ccgtcctggc ccgtggcctg ggcgaggagg ccgggacggg
     2401 cctgccttcc ctcctcggcc tcatccgccg cctcaagtcc ctggtcggcc tcgactacgg
     2461 cctcgcccgg ctggggctca agaccgagga catcccgcgc ctggcgggct tcgccctcaa
     2521 ggacgcctgc ctcgtgacga atccccgggt cttcggcctg gcggagctgg aggcgatcta
     2581 tgccggagcg ctctagcagg gctgcgcccg ggggcacgac gtccgcgccc ggcggcgggg
     2641 ccaacggcgg cgccggccgc accgagggcg acctttcccg cggcgaggcc caggccggcg
     2701 gcggccgcga gcggctccgc gaccgcttca tggggatcga cgagtccttc tcggtgaaga
     2761 gctactatcc ccagctcaag gagcggctcc gcgagctcga ggccgagcgg acccgcgccc
     2821 tcgagagcga ggcccgcctc gccaagctca acacggagct cgagagcctg gtggagagcc
     2881 ggacggctgc cctgcggagc gcgatgtccg gcctgacgga ggccaacgcc gagctcgagc
     2941 gcaccttgag cgacctgcgc gccgcccagg acaggctcgt cctctccgag aagatggccg
     3001 cggtcggacg cctggtcgcg ggcatcgccc acgacgtcaa cagccccttg ggcgccctcc
     3061 tgtcctccgt ctcatccatc gacgagggcc tcgtgggcta cctcgaatcc ttcccctctt
     3121 tctacgcctc gctcgggaag ggcgatttcg gccgctacct cggcctggtc gcggcgggag
     3181 ccgagaaggc gcggagcctc gagtcggggc gcgaccgggc caggaaggac aggctcgctg
     3241 agcttctggc ccccgagtgc ccggacgccc tctccgtcgc cgaggcggcg gaggagctgg
     3301 gcgtcttcga cgaggccgag gcggccgtcg gggagatcgg cttccggcgg gcggcgcgca
     3361 tcatcgcgga ggcggccaag acggtcgcct tgatccgctc cgccgccgtg gccgccgagg
     3421 cgacccgtcg gacggcccgc atcatcgagg ccctgcggcg cttctccctc gggtcggagg
     3481 ccaagtcgag cgagagcatc gccctccgcg aggagttcgc gggcgtcctg tcgctcttcc
     3541 aggacaagtt caagcgcgag ctcgcggtgg acctggacat ggacgaggag atccgggtct
     3601 cgggcgaccg cgaggccctc ggccaggtct ggataggcct ggtcgcgaac gccctccagg
     3661 ccatggaata ccgcggccgc ctgggcctct ccgcgcggga ggagggaggc tccgtcctcg
     3721 tctccgtctc cgacacgggc cgcggcatac gtcccgagga ccgcgaccgc gtcttcgagc
     3781 cctttttcac cacgaagaag ctgggcgagg ggatgggcct cagtctcagc atcgcgcgga
     3841 aggtcgtgga ggccaacggc ggggaaatcg acttcgagag cgagccgggc cgcaccgtct
     3901 tccgggtgag gctcaaggcc gcacggaagt agcctgaggc cgccgccccg agggtcgggg
     3961 tctctggctg accgctcggc cttcctggcc gcggttgtct tcctgagcgc ggcggctgtc
     4021 gctttacgcg gaggggaagc cgtgctaggc tcctcgccca tgcagtccga caacagctcc
     4081 tcttccggcg cctccgccgc gccttcggcc ctagggggcg cggcaacccc gccgcccgcc
     4141 tgggacctct cccccgagta cgcctccttc gacgacccgg ccttcgcctc cgacctcgcg
     4201 cgcgtggagg ccctcgccgc cgagctcgag gccctggcgc ccctggccgc cgccgccctg
     4261 gcccgcgggg ccggcctcga ggccctcgat ccggcctccg agcccggact cgtcgccgcc
     4321 gtcctctcct ccttcgcgat aggcgtcgag atcgacgagc gcttcggcaa tctccgggtc
     4381 tacgcgaact gcgtggcctc ggtggacggc ggcgacgccc gggccaagga gtaccgcggc
     4441 cgcttcggct ccctgggcgc gaggatcgag aaggccgact cggccttcgc cctcctcctc
     4501 gcccgtgcgc cggaggcctt cgtcaaggcc gtcctcgccg cgcccgaggc ggcgggccag
     4561 gccttccgcg tctcccaggc caggaagtac cgcgaccgcc tcctcgagct cgccgaggag
     4621 aagacgatag ccgcccttgc ccccgacggc cgcgacgcct ggggccgcct ctacgactcg
     4681 ctctccggca cggcaaaggc cctctaccgc ggcccctcgc cggagggcgg ggcggacgcg
     4741 tccgcgcccc gcgagatcgg cctctccgag gccgcctccc tgctccgcga ccccgaccgc
     4801 cgggtccgcg aggcggcctt ccgcgcccag acggaggcct tccgcgccca cgaggagagc
     4861 ctcgcggcca tcctcaactc catagcgggc ttccgcctgg ccgagtacgg catgcgctcc
     4921 cggaaggcgc ccatgcactt cctggacgcg gccctggcct ccaaccgcct ggagcgcgcg
     4981 accctggacg cgatgctcgg cgtcgtcgcg gaggagcggg agctcggccg ccgcgccctg
     5041 cgcctccagg cccgcctcat gggcgtcccg cgcctcgcct gctacgacac cctggctccc
     5101 gcgccgcggc ttccgggcgg ggagggcagg gcctactcct tcgacgaggg cctcgagctc
     5161 gtgcgcggcg cctacgcctc cgtcgacccc gcgatgggcg atttcgtcct ggagatgcgc
     5221 gacgcgcggc gcatcgaggc gcgggtcctc cccgcgaagc ggcccggggc ctactgcacg
     5281 cgcttcccca agacgaggac cccccgcgtc ttcctcacct accgcggcgc cctctccgac
     5341 gtctccaccc tcgcccacga gctcggccac gcctaccacg ggaagctcct cgcgagcctt
     5401 ccccttgcgg agaccgagta tccgatgaac ctcgcggaga ccgccagcac cttcgcggag
     5461 acggccttgg gggactacct cgactcgctt gggcagggcg cgggggccgc gggcgaccga
     5521 gccgcgggcg gcgcggccgg gccgggcgga ggcgccgcgg cggacggccc tggctcggga
     5581 gccgcgggcg gcgcgggggc cgcggaagga caagccgcgg gcggcgcggc cgggctctcc
     5641 caggccgaac tagcgggcct cctcgaggtg gcctggtccg acgcccagga cgccgccacc
     5701 ttcctcgtga acatcccggc ccgcttcgcc ttcgagaagg ccttctacga gcggcggtcg
     5761 gagaagcccc tcgggcccgc cgccctctcg gccctcatgg acgagacgat gcgcgagcac
     5821 tacggcgacg cgctatccga gtacgacgcg ggcttctggc gctcgaagct ccacttctac
     5881 atgagcgggc tctccttcta caacttcccc tacaccttcg gctatctctt cagcctcggc
     5941 gtctactcaa ggcgcgccga gctcgggaaa ggcttccacg aggcctactg cgccctcctc
     6001 cgcgacacgg gcaggatgga gaccgaggcc ctcgccgagc ggcacctggg cgtcgacctg
     6061 cgcaagccgg acttctggcg cgcgagcgtg gacatcgtga ggagaaaggt ggaccgcttc
     6121 caggccctgg tggagcggct cgcgccctag acctcgacgg cgagcacggc gacgtcgtcc
     6181 ttggccctgt ccgcgcccat cgcgaggacc agggcctcgg gcagcttcgc gaggggggcg
     6241 ccgcggaggc cgccgacgac gtctaggagg ccgcccctcg agcgcttcca ggcctccggg
     6301 ctcgggccgc actccacgag gccgtccgtg aagaggacga agcggtcgcc ctcctcgacc
     6361 tccatcgact cggagcggta ggtcgactcc tcgatcatgc cgatcatcgg gttctcgcac
     6421 tccaggaagc gggcctcgcc gcgcgcgggc aggtagaggg ccgaggggtg gccggccgcc
     6481 atgtacacag ccttcctcga gcggcggttc aggcgcaggg cgtaggcggt gaggtaggtg
     6541 tcggcgcaga cggtctggga gaggacgccg ttcatgaggc cgacggtctc ctcgagggag
     6601 taggccggcg tcgagaactg cttgaggagg accttcaccg ccggcgtgac gtagctcgtc
     6661 cccaggtcgt ggcccgcgac gtcggccatg aggtagagga agacgtcctc ggcgacgggc
     6721 acgacgtcgt agaagtcccc gcccgcctcg tggaggctcc ggtagtagac ggagaagcgg
     6781 gccttgggca gctcctgggg gcgcacgagg agggagcgct gggcctgggc tatctgcatg
     6841 atgctctccc gccgggcggc gacgagctcc tcgttcgcga gggccagtcg cacgtggacg
     6901 cggaccctgg cccgcacctc catcgggttg gccgacttca cgatgtagtc gacagccccg
     6961 agctcgaagg ccttgagctt cgagtcctgg tcctccaggc tcgtgagcat gatgacggga
     7021 gtgcgcgccg agcccggctc ctccttgagc cgcctcaggg tctcgaagcc gtctatgccg
     7081 ggcatgacca cgtccaggag gatgaggatg ggcttttccg ccccggcgga cttgaggcag
     7141 gcctcgccgt tcgcggcctg gaccacgcgg tagccgtcct cctccaggat gtacttgagg
     7201 tagctccggt tcgtggccga gtcgtcgacg acgagtatgg tgtaggaaac ggcgcccatg
     7261 ctccccccag ggggaaagta taagcgggag gcgcggctcg gcgccagggg aaagccgacg
     7321 gcgccagggg caagccgacg gcgccaggga aaagccggag cgccggagga aggggggcga
     7381 gtttccgccg cccgcgcgct atacttgccc ccgtggccga agaagagcta gaagaccagg
     7441 tcctcgtcca tcggagcctc gcgggagacg aggtggcctt caggcgtctc gtcgagcgct
     7501 acgccgaccg cgtcctgcgg ttctgcgagg ctcgcctagg ctcgggcgag gacgcggccg
     7561 acgccgccca ggaggtcttc atccgggtct accgctccct cggaagctac aggctcggcg
     7621 cgagcttccc cgcctggctc ttcgccatcg ccgcgaacag ggtcaggacc aggtggaccc
     7681 gggccgcgac cgaacggaaa aaaatcgagc gatccgcggc cgaggccgag ggcgcctcct
     7741 cgcccgatcc cgaggacgag gccctgcgct ccctccaggg cgaggcggtc aggtcggccg
     7801 tggcggccct tccgaaggag cagcgggatt gcgtggaact ctactatttt ggcggcctgg
     7861 gcgtggcgga atgcgccgag gcgctcggcc tgggcgcgga ggccgtgaag tcccggctgt
     7921 tcagggccag gcgcacgctt cgggcggcgc tcgaaaccgg gcaaccgaag cccccttccg
     7981 agggtatact atgacgatgg actgcaagga agtcgaagga ctcctccgct cctgggagga
     8041 gggccggaat ccgcctgagg ccgcgctcaa ggagctcata gggcacatcg tgtcctgcgc
     8101 ctcctgctcc gcggcccgcg ggggcatcct tcccctgctc cgcctcgagc tcgggcgcga
     8161 ggcccgggag gaggccggcc ccacggcccc gagcgccgcc gcacggtcct tggccgacgg
     8221 agtgatggcc cgtctttcct ccttggaggc gaaggcgccg gcttccggag ggcgacgccc
     8281 gaccaggccc gtcctggtcc tccaggcgga catgccgcgg agaaagcgcg tcgcttggcc
     8341 cctcctcgcg acggcggccg ccgccctcct cgtcgccggc cttggcttcg gactccactt
     8401 cttcggaccc ggggacggag gagtcgtgac cgtccgcttc gtgctcgacg ctccggaggc
     8461 gaagtcggtc tatctcgccg ggaatttctc ggactggaag ggcgagagcc tccccctcga
     8521 gcgcaagacg ccgggagagg cctgggagct caccttgaag ctgcgcaagg acaacatgta
     8581 cgtgtacaat ttcatcgtgg acggcgagcg ctggatcgtc gatccgtcgg tgcccgagac
     8641 cgtcgaagac ggcttcggcg gctcgagcag cctcctcagg ctctaggggg gaacatgaaa
     8701 gccttcatcg gcgcgaagcg cctcatgtac gccgcggcgg ccgcggcctt cgtcctcgcc
     8761 gcggcaggcg ccgcctcggc ggcggaatcg gcccccgcgg ccggcgcggc cggagcggcg
     8821 ttcgagaccg gcgccgcctc ggcgccaggc tcgggagacc agtcttcgga cgaagcctgg
     8881 gacgaagcct tcgcgctggc ctacgccgcc gccctcgccg gcgcgccctt cgagccctct
     8941 gccctggccc tgcggatcgt cgaggcggag acaagcgcct tgcggcggga ttggccgccc
     9001 gaggccgccg cctcaagagt cctggaactc tcgctttgcg cggagagcgc cttgcgtttc
     9061 gggtcgagcc cccaggagac cagggccctc atccgcgcgc gcctccgctt cgacggcccg
     9121 gggccttcgg cctcccgctt ctcgaaggaa ttgggtagga gcctcaaggc ctcggcctca
     9181 ggcggcgcca agggggccgc ggcacccggc ggcttcggcg gcttcggagg gcggggccgg
     9241 cgatgagcct cctcagggcg cgcctccttc tcgtcccgct cctcctggcc gcggcctccc
     9301 tggccgcggg gcaggggacg cgcgactggt tcgaacgcga ccccgaggcg gcggcctacg
     9361 cgggcatacg ctccggcgtg gaagccctct ccctccgcgc tcagggagag ggcctgcctg
     9421 agagccttct cctgcgcagg ctcgtcgagg gcgccgcgaa gcgcgcagag cccgaacgcc
     9481 tccttctcgc gctttccgag gagacggagc gcctggtcgc gctcaagaag gcgctcggcg
     9541 ggcggggcct cctcccccgg gacccggcca aggccgaagg gatcctcgcc gagggctcgg
     9601 tcatcctgag ggcgggcgtc ggcctgcccc tccttctttc cgccgtggac gcctcgcaaa
     9661 gcggcggggc cggtccggat gcctccgcag cccgggccta cgccgcccta ggcgcggcga
     9721 tatcggtcaa cgcgcgcttt cccttgggag aggccgcctt ccgggcactc gccctcgccc
     9781 tggccggaag ctccctgccc gaaggccgct tcggggcctt cgtcgcggtc ttcgcgaggg
     9841 ggcgggcctc ggggctggcc gccgacagga tcgcggaggt ctccgtggcc gtcctggacg
     9901 ccggaggctc gctggaccgg gtggagaaag agctggaacg gaggatgaga aagccatgac
     9961 ccgactcgca gcccgccgct tcgcggtctt gaccgccctg ctctcgctgg cggcctcccc
    10021 ctccctagcc gaggccgtcc tcggggcggg gatggtctac gacgaggctt ccggcccctc
    10081 cctcgccgct tcggcgcaag gcgccgtgga gacgatcggc tcccgcctag ccctaaggct
    10141 ggagggagaa ggcgaagcct ccgtcgccct ccctgccccc ctcggagccg cgcccttcga
    10201 tccctcggcg tccggggcgg aggcctttgg cctcgtcgaa ctctcctaca cggcgggctc
    10261 actgaactgc ctgggcgcgg cgagcggcgg actatccctg gaagaaggcg cggtctcgta
    10321 cagcgcgggg cttcggggcc gcgtcgcggt ccaagccctg gagctctccg cctccctctc
    10381 gccccgcctg ggtttcgatt cgtcggcgca agggggactg gagcttggcc tccttggcga
    10441 gtgttccttc ctggccgcgg acctggtcct gaagccgcgc ctggaccttt cggccttcga
    10501 cttccgcgac ggttcccggg ccttccgcgc gatgcccggc ctcgccctgt cctggtatcc
    10561 gctcgttcct ctctcgatct ccttcgaggc gggctaccgg cgggaatgga cggcggaagg
    10621 cgccgcgacc tcggggatcc ccttgagcct ctcgcttttc gcctctcccc tcgacaaggt
    10681 cttccttagg gcgggagccg aggccctggc gccgctcccc tcgggtgccc tgtcggacgc
    10741 ggccgcctat ctcgagctgt ccttcgccct ggcggggcgc gcgggaaggg agctccggct
    10801 gcctctgcgc ctctcctacg acggcgacgc cgaagcgcgc tacagcctct acgcgggact
    10861 agagctttcc ttcgattgag cggaccgtcg ccacgggctg cgtcacgtcg cctcggcggc
    10921 tacggctccg gcgaattcga cagggccgcg caaccggcgg cggaaacggg gggtatcctt
    10981 catgccggat cgagacggtc caggcaggag gatttcatga aacgactggc aacgctggtt
    11041 ttattggcgg tcatcgtcct cggagcgggc gcgcagacga tcgggctcga ggagccggcc
    11101 ctggaggcgg ccatacgcga gatgaacggg atcccccagg agcagctcgc cgcgtactac
    11161 gcggaccttg ccctgcggta tcggttcaag gcgaaggaca tcgagcgcct cgcgctccgc
    11221 tacaggctcg aggccggcga agtctgcctc gtcgccttca tggcccggac ccgctccatg
    11281 accatggagc gcgtggcgaa gatgaagact cccgaccaga cctggggaga ggtggccact
    11341 aggctcaggc tcaaccaggg ttcgcctgag ttcggcaagc tcaaggaaga cgctgagggc
    11401 gacctcctgc gcgtccgcga cagggagcgg ctcaggcttg agacgcagac gcagctccag
    11461 acccagacac agctccagac gcaaactcag acccaactca agacccagga tggcgagggc
    11521 tcaggctcgg gtgcggggac cggcaaaggc aagtgaagga agggcgtcgc atggcgccct
    11581 cgcgataggg ccaggttcct cctcgagcct ggcccgcccg acttcttccg cccaggcgga
    11641 aggagccggg tttttcttgg aaagcgcc
//
LOCUS       DAOOSJ010000055        11578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3694_length_11578_cov_5.177731, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000055 DAOOSJ010000000
VERSION     DAOOSJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3694_length_11578_cov_5.177731"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLG14_04255"
     CDS             <1..392
                     /locus_tag="PLG14_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOX48377.1"
                     /translation="AGQKAAYAEYSGLVESGLAREIARIDLPLSLYTEWYWQIDLHNL
                     FRFLALRLDSHAQKEIRAYAEVLLEVSRRVAPAATESFERHQLTGLRFSGPEAEELRA
                     RLSGGKGSLSGKELERFEEKLKSGKQL"
     gene            complement(485..1534)
                     /locus_tag="PLG14_04260"
     CDS             complement(485..1534)
                     /locus_tag="PLG14_04260"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOX48378.1"
                     /translation="MAANGFPDPPEAAVTRGSFELGRFNAPFPRANMLDVARPYHYAV
                     PRFVKNLRLKEWQAFQFGDERWFFFSALYNAKLMSLAIFHAYDRKTRRRHSIQRVIPG
                     SAFPFGERLSGSRLSYRGARSRLEYACLLDEGAIEVRASRSHRDAARAFEGSFRFSYG
                     PKACAPSAACLPLGLNRAMYSMKTLMPLTGEFQAGGETFRLDPPGAMGVIDEHKGFYP
                     WQMRYDWVTGFGLDAKGRRVGFNLTSNQVRDQARYNENCLWINNKVWPLPPVRVTRPR
                     GPEGEWIVQDTEGMVDLVFVPEARNDVRFNLGFAKSDYHGPFGSFRGVLKNGEGEKIE
                     AERLYGAGEQKYLRL"
     gene            complement(1544..2428)
                     /locus_tag="PLG14_04265"
     CDS             complement(1544..2428)
                     /locus_tag="PLG14_04265"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX48379.1"
                     /translation="MDPAQDVLGFVGIGVMGKSMAGHLLKAGYRVLVYTRTKDKARDL
                     LSLGAEWAESPAVLAPRCKAVFTMVGYPSDVEEVYFGPKGLIENARPGSFLVDTTTSK
                     PELARRIAQAAKARGVAALDAPVSGGDVGARNAALTIMVGGERADFEAVRPLLEVMGK
                     TVILQGPAGAGQHTKMANQIVIAGNLAGAVEALCYSRAAGLDPRTVLASIGAGSAGSW
                     QLNTMAPRMLDGDFAPGFYVKHFLKDLRIALEAAKEMKLKLPLLSLAEGLFARLSNEG
                     FGDSGTQALYLLYERGLV"
     assembly_gap    2481..2580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      2811..3095
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3119..4270
                     /locus_tag="PLG14_04270"
     CDS             3119..4270
                     /locus_tag="PLG14_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX48380.1"
                     /translation="MRRIILSVLVLISIQAAALAAPTAPAAQAQAQAQARIRYATGFT
                     VQYREGYKLVTVTKPWPGAAEAYEYVLYPRGSRKPDYRPSGAAKPRFVETPLRRVVSY
                     SSTYLPQILAAGGLESLVGVDNASYVSSPEVRARVAAGKTVETSRNWAPNVELLLSLA
                     PDGIFAYGVGNEWDFHPKLEEARLPVIMDGEWNEAEPLARAEWMKFIALFYDAEAEAE
                     ARFAAVEKEYLRLKALAASSPRRPKVLVNAPFQGVWTLSGGKSYQARFIADAGADYLW
                     ADDPSSGGISLSVEAAYERGSKAEVWINPGAAKSLAELAAMDRRFAALPPLARGEVYG
                     NVRRLNEAGANDYFESGVMRPDLVLADLVRLFHPELGLPGEPYYYLRLK"
     gene            4267..5310
                     /locus_tag="PLG14_04275"
     CDS             4267..5310
                     /locus_tag="PLG14_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOX48381.1"
                     /translation="MRKIRPRLLALAGAVLLLFALDLLSGSVRIPLAEALGILLGRAA
                     ENPVHGTILLLFRLPKALVALAGGAALAASGLLLQTVFRNPLAGPDVLGINSGASVGV
                     AFLVLAAGGSGAAGASSLLGSLGLAGYALLALAASLGAGLVLGLILLLSRRFEDAVTL
                     LILGMLVGYVAGAAVSLLVYFGAPSKVQLYLSWTYGSFGGVTVRELPVFLGAIGLGFL
                     LLLRAGKPLNALLLGERYAASLGVDVRRERGRAIAAAALLTGAATAFCGPIAFVGIAA
                     PQAARRFLKSSDHGLLIPASALTGALFCLGADVVAQLPRNGSVLPVNPLLALVGAPII
                     LSVFMRRSAEERP"
     gene            5307..6398
                     /locus_tag="PLG14_04280"
     CDS             5307..6398
                     /locus_tag="PLG14_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48382.1"
                     /translation="MSGPILEARSLSIGYPARAGGRERLVAAGLDFALSRGDFVCLLG
                     ANGAGKTTLLKTVAGMQPPLSGECRIEGKPVSRLGLEERAGLVALVLTEQPGGGWFSA
                     YEIAAFGRYPYTDSRGRLSAEDRKAVLGALAAVGMEGFAARPFAELSDGEKRKILVAR
                     ALAQDTPLLILDEPTVFLDAPSRLELFYLARRLAREAGKAVVLSTHDVELALAKADRL
                     WLLGKGGSFAEGLPEELALSGKLGEALSTEAVRYDAESGGFRPAGASPQPAIALVLEA
                     AAAPHGEAAAEDHALALWARRLVSRLGYRPAGPGAKTALRLRLFPAAAPGAEPRWRIE
                     REGAEPREGSGLAALARALEAGQETVRKG"
     assembly_gap    6530..6629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6630..8198)
                     /locus_tag="PLG14_04285"
     CDS             complement(<6630..8198)
                     /locus_tag="PLG14_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48383.1"
                     /translation="MRSITAKIIVLAATAVILVAGVLAGTFVVVLSRSSEAAVASLER
                     TLREDFDLLIKSEVETAASMLKRIAKLRDEGKLSEREADETARALLRDIRFGADGYFW
                     ADTKEGVNVVLLGGASEGKSRIDLKDVKGFELVKAIIAAGVAGGGYTDYWFPRAGETE
                     ALPKRGYSLLVPEFGWVVGSGNYIDSIDKIAAAKRAEAAAARRAALVLVLLIAASFAA
                     AAVGVSVFVGRRISRPLVYATERVRRIASGDLTGAFDAAFLARKDEAGQIVQGLSEMR
                     DDLAALIKGAGDSARRVAKGSEEFKAAAEAIAEGSSRQAAGAEEVSSSVEELTATARR
                     NAEGARETSGIALESSKEASSSEAAFEKAAAALHSIADRISVIEDISRQTNMLALNAA
                     IEAARAGESGKGFAVVAQEVRKLAERSRLAAEEIRGISADTQASSDLAKAALGRLVPG
                     IVKTAELVEEIGASSREQEAGTDQIAKAVVELDTVVQRNAAAAEELAASANSLADEAH
                     RLEASVASFKVDEEA"
     gene            8424..8942
                     /locus_tag="PLG14_04290"
     CDS             8424..8942
                     /locus_tag="PLG14_04290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOX48384.1"
                     /translation="MRYSVVKKQNNSRKCFVCGLGNDSGLHAAFYETEAGELVALVRP
                     EEEHQSYPGRMHGGVAAALLDETIGRAIAIGKDEDVWGVTVELTTKYRKPLPLGQELK
                     VVGRVTKDGSRFFEGSGEILLENGDVAVQATGRYLKVPIGKITDSGMGEADWFLAPAK
                     EDPEAIELPGRD"
     gene            complement(9107..10096)
                     /locus_tag="PLG14_04295"
     CDS             complement(9107..10096)
                     /locus_tag="PLG14_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOX48385.1"
                     /translation="MTRAERAAAIHASGSSCAQAVFAAFAEDLGLDEGTAQRIASGLG
                     AGYGRLQLTCGAVSGGVLALGLALGNARGEEQDRKEAAYAASRELALAAAAEFGASDC
                     RALLRGADLRTEEGKARVKAEGLSDLVCKPIVGRCVELVEERLAAAGLIRQAPAGGLG
                     ADGPRGGGGRSLPEGLAIEAAGPAEAGLLGEFRARMFAEMDEANAVPAVRAAVASGAA
                     EYFRRKAGQPDQVNLVARLGGRVVGCAAMILEERPARPGDGRPLGGFVHNVYVLPEAR
                     GKGVARALMERLRAEAEARGVSRLSLRASAAGRPLYLGLGWAQNPKDLELKLT"
     gene            complement(10108..10971)
                     /locus_tag="PLG14_04300"
     CDS             complement(10108..10971)
                     /locus_tag="PLG14_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HOX48386.1"
                     /translation="MSDKIMMAALAAERNEITEFHIYSRLAAGCRDAKNAEVLSRIAA
                     EEKKHAEFWAAKTGVAPRPNRLRVFRVVLLARLLGLTFVLKQMERNEGTASRRYADLG
                     TAYPETARLSAEEGAHEKALLGMLDEERLQYVGSVVLGLNDALVELTGALAGFTLALA
                     DTKIISLAGLVTGISAAFSMAASDYLSSKAENDPRAAKSALYTGVAYFFTVILLILPF
                     LLLSTKILALGITLATAVLIIFLFNYYLAVAKDIDFKRRFLEMTFISLGVSAFSFLIG
                     FVLKGVLGVDA"
     gene            complement(11102..>11578)
                     /locus_tag="PLG14_04305"
     CDS             complement(11102..>11578)
                     /locus_tag="PLG14_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOX48387.1"
                     /translation="GEVFMVPAFVGMGAPYWDSEVRGTVVGITRGTTRAHFVRAALEA
                     IAYQSRDLLVAMEKDFGRPISCIKADGGASANNFLMQFQADVLDRSVVLPEVGEITAL
                     GAAYLAGLSVGYWESLAEVERNWRRKREYLPEMAEEKRERLLASWDKAVASARSYK"
BASE COUNT         1549 a         4252 c         3953 g         1624 t
ORIGIN      
        1 aggcggggca gaaggccgcc tacgccgagt actcgggcct cgtcgagtcg ggcctggcgc
       61 gggagatcgc ccgcatcgac ctgccactct ccctctacac cgagtggtat tggcagatcg
      121 acctccacaa cctcttccgc ttcctcgccc tgcgcctgga ctcccacgcg cagaaggaga
      181 tccgcgccta cgcggaggtc ctcctcgagg tctcgcggcg cgtcgccccc gccgcgacgg
      241 agagcttcga gcgccaccag ctgacgggcc tgcgcttctc cggccccgag gcggaggagc
      301 tccgcgcgcg gctttcgggc gggaagggga gcctctcggg caaggagctc gagcgcttcg
      361 aggagaagct caaatcggga aagcagctct aggaaaaggg cgtagccgcg cgggcgccta
      421 cgtctggacg aggctcgcct aggcggcgcg gaccggcgct cggcccgcgg cggcccccag
      481 ccggctagag cctgaggtac ttctgctcgc cggccccgta gagccgctcg gcctcgatct
      541 tctcgccctc gccgttcttg aggacgccgc ggaaggagcc gaagggcccg tggtagtcgc
      601 tcttcgcgaa gcccaggttg aagcggacgt cgttcctggc ctcggggacg aagaccaggt
      661 ccaccatccc ctcggtgtcc tggacgatcc actcgccctc ggggccccgg ggccgcgtca
      721 cgcggacggg cggcaggggc cagaccttgt tgttgatcca gaggcagttc tcgttgtagc
      781 gagcctggtc gcgcacctgg ttgctcgtga ggttgaagcc gacgcggcgg cccttcgcgt
      841 ccaggccgaa gcccgtcacc cagtcgtagc gcatctgcca ggggtagaag cccttgtgct
      901 cgtcgatgac gcccatcgcg ccgggcgggt ccaggcggaa ggtctcgccg ccggcctgga
      961 attcgcccgt gaggggcatg agcgtcttca tggagtacat ggcccggttc aggccgaggg
     1021 gcaggcaggc cgcgctgggc gcgcaggcct tgggtccgta ggagaagcgg aagctgccct
     1081 cgaaggcgcg ggccgcgtcg cggtggctcc gcgaggcgcg tacctcgatg gccccctcgt
     1141 ccaggaggca ggcgtactcc aggcggctcc gcgcgccgcg gtaggagagg cgcgagcccg
     1201 agagccgctc cccgaagggg aaggcggagc cggggatgac gcgctggatc gagtggcggc
     1261 gcctcgtctt ccggtcgtag gcgtggaaga tcgcgaggct catgagcttc gcgttgtaga
     1321 gggcggagaa gaagaaccag cgctcgtcgc cgaactggaa ggcctgccac tccttgaggc
     1381 ggaggttctt cacgaagcgg ggcaccgcat agtggtaggg cctcgccacg tccagcatgt
     1441 tggccctcgg gaagggcgcg ttgaagcgcc cgagctcgaa ggagccccgg gtcaccgccg
     1501 cctcgggggg atcgggaaag ccgttggccg ccatccgccc tctctagacg agcccgcgct
     1561 cgtagaggag atagagggcc tgggtgcccg agtcgccgaa gccctcgttc gaaaggcgcg
     1621 cgaagaggcc ctcggccagg gaaaggaggg gaagcttgag cttcatctcc ttggccgcct
     1681 cgagcgcgat ccttaggtcc ttgaggaaat gcttcacgta gaagccgggg gcgaagtctc
     1741 cgtccagcat gcggggcgcc atcgtgttga gctgccagct ccccgcggat cccgcgccga
     1801 tgctcgccag gaccgtccgg gggtccaggc ccgcggcgcg cgagtagcac agggcctcca
     1861 cggcgcccgc caggttgccc gctatgacga tctggttcgc catcttcgtg tgctgccccg
     1921 cgcccgccgg gccctggagg atcaccgtct tccccatgac ctcgaggagg ggcctcaccg
     1981 cctcgaagtc ggcccgctcg ccgccgacca tgatggtgag ggcggcgttc ctggcgccca
     2041 cgtcgccgcc ggagaccggc gcgtccaggg ccgcgacgcc gcgggccttg gccgcctggg
     2101 cgatccgcct cgcgagctcg ggcttggagg tcgtcgtgtc gacgaggaag gagccgggcc
     2161 tcgcgttctc gatgaggccc ttcggcccga agtagacctc ctcgacgtcg ctcgggtagc
     2221 ccaccatcgt gaagacggcc ttgcacctgg gggccaggac ggcggggctc tccgcccact
     2281 ccgcgcccag ggagaggagg tcgcgggcct tgtccttcgt cctcgtgtag acgaggacgc
     2341 gatagccggc cttgaggagg tgccctgcca tgctcttccc catgacgccg atgccgacga
     2401 agccgagaac gtcctgcgcg ggatccatat ccaaagccct ccgttgcggt cgagtggact
     2461 tatatcctag cgtgccggcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 catatccaaa gccctccgtt gcggtcgagt ggacttatat cctagcgtgc cggccagctt
     2641 cgggcaacag gagggggcgt tcctccccgc agagccgccc gcgcgccgcc ggccccggcc
     2701 agggagccgc gaaccgcggg ggcccgcgta ggccctcgga ggcaagcgcc gtcgccctcc
     2761 gcccccgcgc cgccggcccc gcttgcctcc cccgcctgag gcggagtagg ctggacttca
     2821 cggtttcggg ttccttcccc cggcgcgtcg cggcgggggc gggtgaaaag ggaaggcggt
     2881 gcggccaagg gccggcgaag ggagagctcc ctcgcccagg actcgggcca attccgccgc
     2941 agcccccgct actgtaagcg ccgtcgcgcg ggaatcggcc actgggcagg cagcccggga
     3001 aggcgcccgc ccgcccgccg tccctcgcgc tcaaaaagcc ggggatgggg ggcgcgccgc
     3061 gagccaggag acctgcccga agccctcgct ttccaactct tcggagggag gaggaggcat
     3121 gcgcaggata atcctgtccg tcctcgtatt aatttccatt caggcggccg ccctggccgc
     3181 gccgaccgcg cccgccgccc aggcccaggc ccaggcccag gccaggatcc gctacgccac
     3241 gggcttcacg gtccagtacc gggagggcta caagctcgtc acggtcacca agccctggcc
     3301 gggcgccgcg gaggcctacg agtacgtcct ctacccccgc ggctccagga agcccgacta
     3361 ccgcccctcg ggcgcggcca agccccgctt cgtggagacg ccactgcgac gcgtcgtgtc
     3421 ctactcctcg acctacctgc cccagatcct cgccgcgggg ggcctcgaga gcctcgtggg
     3481 cgtcgacaac gcctcctacg tgagctcccc cgaggtccgc gcccgcgtgg ccgccggcaa
     3541 gaccgtggag acctcccgca actgggcgcc caacgtggag ctcctcctct ccctcgcccc
     3601 cgacgggatc ttcgcctacg gcgtcggcaa cgagtgggac ttccacccca agctggagga
     3661 ggcccgcctt cccgtcatca tggacggcga gtggaacgag gccgagcccc tggcccgcgc
     3721 cgagtggatg aagttcatcg ccctcttcta cgacgctgag gccgaggccg aggcccgctt
     3781 cgccgccgtg gagaaggagt acctccgcct gaaggccctg gccgcgtcga gcccccgccg
     3841 gcccaaggtc ctcgtcaacg cgcccttcca gggcgtctgg accctctccg gcggcaagag
     3901 ctaccaggcc cgcttcatcg cggacgcggg cgcagactac ctctgggccg acgatccctc
     3961 ctcggggggc atctccctct ccgtggaggc ggcctacgag cgcggctcca aggccgaggt
     4021 ctggatcaac cccggcgcgg ccaagagcct cgccgagctc gcggccatgg accgccgctt
     4081 cgccgccctc ccccccctcg cgaggggaga ggtctacggc aacgtccgcc gcctgaacga
     4141 ggccggggcc aacgactact tcgagtcggg cgtcatgcgc ccggacctcg tcctcgccga
     4201 cctggtccgc ctcttccacc ccgagctcgg cctccccggc gagccctact actatctgag
     4261 gctcaagtga ggaagatccg gccgcggctc ctcgccctcg ccggggccgt cctcctcctc
     4321 ttcgccctcg acctcctctc gggctcggtg aggatccccc tcgccgaggc cctggggatc
     4381 ctccttggcc gggcggcgga gaaccccgtc cacgggacca tcctcctcct cttccgcctg
     4441 cccaaggccc ttgtcgcgct cgcgggcggc gcggccctgg ccgcgagcgg cctcctcctc
     4501 cagaccgtgt tcaggaatcc cctcgccggc cccgacgtcc tcggcatcaa ctcgggggcg
     4561 agcgtcggcg tcgccttcct cgtcctcgcg gcggggggct cgggcgccgc gggggcttcg
     4621 agcctcctcg ggagcctcgg cctcgcgggc tacgccctcc tcgccctggc cgcgagcctc
     4681 ggcgcgggcc tcgtcctcgg cctcatcctc ctcctctcgc ggaggttcga ggacgcggtc
     4741 accctcctca tcctgggcat gctcgtcggc tacgtcgcgg gggccgccgt ctccctcctc
     4801 gtctacttcg gcgcgccctc caaggtccag ctctacctct cctggaccta cggctccttc
     4861 ggcggcgtca ccgtccggga gctccccgtc ttcctgggag cgataggcct gggcttcctc
     4921 ctccttctcc gggcgggaaa gcccctcaac gccctcctcc tcggggagcg ctacgccgcg
     4981 agcctgggcg tcgacgtgcg ccgcgagcgt ggccgggcca tagccgccgc cgccctcctc
     5041 accggcgccg cgacggcctt ctgcggcccc atcgccttcg tcggcatcgc ggcgccccag
     5101 gccgcccggc gcttcctcaa gagctccgac cacggcctcc tcatcccggc ctcggccctg
     5161 acgggagccc tcttctgcct gggcgccgac gtcgtcgccc agctcccccg gaacggctca
     5221 gtcctcccgg tgaaccccct cctcgccctc gtgggcgcgc cgatcatcct ctcggtcttc
     5281 atgcgccgca gcgcggagga gcgcccgtga gcggccccat cctggaggcc aggagcctct
     5341 ccatcggcta ccccgcccgc gcgggcggcc gggagcgcct cgtcgcggcc ggcctcgact
     5401 tcgccctttc gcgcggcgac ttcgtctgcc tcctgggcgc gaacggcgcg ggcaagacga
     5461 ccctcctcaa gacggtggcc ggcatgcagc cgcccctctc gggcgaatgc cggatcgagg
     5521 ggaagcccgt ctcgcgcctc ggcctcgagg agagggccgg cctcgtcgcc ctcgtcctca
     5581 cggagcagcc cggcggcggc tggttcagcg cctacgagat cgcggccttc ggccgctacc
     5641 cctacacgga ctcgcggggc cgcctctctg cggaggacag gaaggctgtc ctgggcgccc
     5701 tcgcggcggt cggcatggag ggcttcgccg cccgcccctt cgcggagctc tccgacggcg
     5761 agaagcgcaa gatcctcgtg gcccgggccc tggcccagga cacgcccctc ctcatcctgg
     5821 acgagccgac cgtcttcctc gacgcgccct ccaggctcga gctcttctac ctcgcccgcc
     5881 gcctggcccg cgaggcaggc aaggccgtcg tcctctccac ccacgacgtg gagctcgccc
     5941 tcgcgaaggc ggacaggctc tggctcctcg gcaagggggg aagcttcgcg gagggcctcc
     6001 ccgaggagct cgccctctcg ggcaagctgg gcgaggccct ctccacggag gcggtgcgct
     6061 acgacgccga gtcggggggc ttccggccgg cgggagcctc gccccagccc gccatcgccc
     6121 tcgtgctcga ggcggcggcg gcgccccacg gcgaggccgc ggcggaggac cacgccctgg
     6181 ccctctgggc caggcgcctc gtctcgcgcc tcggctatag gccggcgggc ccgggcgcca
     6241 agacggcgct ccgccttcgc ctcttccccg ccgccgcccc cggcgccgaa ccgcgctggc
     6301 gcatcgaaag ggaaggcgcg gagccccggg aaggctcggg cctcgccgcc ctggcccgcg
     6361 ccctcgaggc tggccaggag acggtccgca agggctagac cgccgcggcc tggcggcgcg
     6421 ccgctacgcc ttcagtactt gtacccgaag cgccggagca tcgccttgcg gtctgccttg
     6481 tcggcctcgc cctcgacgcc cttgggggag gagccgtcca cgacgcccan nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcctcctcat ccaccttgaa ggaggcgacg
     6661 gaggcctcga gcctgtgcgc ctcgtcggcc agggagttgg cggaggcggc cagctcctcc
     6721 gcggccgcgg cgttgcgctg gaccaccgtg tccagctcga cgacggcctt cgcgatctgg
     6781 tcagtgcccg cctcctgctc gcggctggag gcgccgatct cctcgacgag ctcggcggtc
     6841 ttcacgatgc cgggcaccag gcgccccagg gccgccttgg ccaggtcgga ggaggcctgg
     6901 gtgtcggcgg agatgccgcg gatctcctcg gcggccaggc ggctccgctc ggcgagcttg
     6961 cgcacctcct gggcgacgac ggcgaagccc ttgccgctct cgcccgcgcg cgccgcctcg
     7021 atggccgcgt tgagcgcgag catgttggtc tgccgggaga tgtcctcgat gacgctgatg
     7081 cggtcggcga tggagtggag ggcggccgca gccttctcga aggccgcctc gctggacgag
     7141 gcctccttgg aggactccag ggctatcccg ctcgtctcgc gcgccccctc cgcgttcctg
     7201 cgcgccgtcg cggtgagctc ctccacggag gaggatacct cctcagcgcc ggccgcctgc
     7261 ctgctcgagc cctcggctat ggcctcggcc gccgccttga actcctcgct gcccttcgcg
     7321 acgcggcggg cggagtcgcc cgcgcccttg atgagggcgg ccaggtcgtc gcgcatctcc
     7381 gaaagcccct gaacgatctg cccggcctcg tccttccgcg ccaggaaggc cgcgtcgaag
     7441 gccccggtca ggtcgccgga ggcgatgcgc cgcacccgct ccgtcgcgta gaccaagggg
     7501 cgggagatgc gcctgcccac gaagacggag acgccgacgg cggccgcggc gaaggaggcc
     7561 gctatgagga ggaccaggac cagggcggcc cgccgcgccg ccgccgcctc ggcccgcttg
     7621 gccgcggcga tcttgtcgat ggagtcgatg tagttgcccg agccgacgac ccagccgaac
     7681 tcgggcacga gaaggctgta gccgcgcttg ggcagggcct ccgtctcgcc ggcccggggg
     7741 aaccagtagt ccgtgtagcc gcctcccgcg acgccggccg cgatgatcgc cttcacgagc
     7801 tcgaagccct tcacgtcctt caggtcgatg cggctcttcc cctcggaggc gccgcccagg
     7861 aggacgacgt tgacgccctc cttcgtgtcg gcccagaagt agccgtcggc cccgaagcgg
     7921 atgtcgcgga ggagggcccg cgccgtctcg tctgcctccc gctccgagag cttcccctcg
     7981 tcgcggagct tcgcgatgcg cttgagcatg ctcgccgccg tctccacctc ggacttgatg
     8041 agaaggtcga agtcctcccg cagggtcctc tcgagcgagg cgacggcggc ctccgacgag
     8101 cgcgaaagga cgacgacgaa ggttcccgcg aggacgcccg cgacgaggat gacggccgtg
     8161 gcggccagga cgatgatctt ggcggtgatg gaacgcaagg cttgctcctt ctatcatatg
     8221 gccgcggcgg aaggcgctac gggctgctgt aagtataggc ccgacgctcc gggccgcaag
     8281 cccgcgaact ccggacgccg gggcgcacct ctcggatgcc gggcgccggc ccgctccgcc
     8341 cgccccattg cgacaaagcg acatattgtg tacaccgtgt cggtcacggg cgcgacaggg
     8401 cgtccgcgcg agggagaaac gccatgcgct attcggtcgt gaaaaagcag aacaattcca
     8461 ggaagtgctt cgtctgcggc ctcgggaacg actcgggcct ccacgcggcc ttctacgaga
     8521 ccgaggccgg cgagctcgtc gccctcgtgc ggcccgagga ggagcaccag agctaccccg
     8581 gccgcatgca cggcggtgtc gccgccgccc tcctcgacga gacgatcggc cgggccatcg
     8641 ccatcggaaa ggacgaggac gtatgggggg tcaccgtcga gctcacgacc aagtaccgca
     8701 agcccctccc cctgggccag gagctcaagg tcgtgggccg cgtgaccaag gatggctcgc
     8761 gcttcttcga gggttcgggc gagatcctcc tcgagaacgg cgacgtcgcc gtccaggcga
     8821 cgggccgcta cctcaaggtc ccgataggca agatcaccga ctcggggatg ggcgaggccg
     8881 actggttcct cgcccccgcc aaggaggatc ccgaggccat cgagctgccc ggtcgcgact
     8941 gagcggcgcg cgcggcccag gccgtggcgc gccgccggga gtgacggtgg ccgcggccga
     9001 gcggccgacg ccggggcacg cgactggcga catcgcataa gcgatgcacc gccggcgccg
     9061 gccagtccgg cgcgccgccc gagcggcggc gcgcggccgc acttcgctat gtaagcttga
     9121 gctccaggtc cttggggttc tgcgcccagc ccaggcccag gtagaggggc cgcccggccg
     9181 ccgaggccct gaggctcagc ctcgaaacgc cgcgggcttc cgcctcggca cggagccgct
     9241 ccatgagagc cctggccacg cccttccccc gcgcctccgg aaggacgtac acgttgtgga
     9301 caaagcctcc caggggccgt ccgtcgccgg gcctcgcggg ccgctcctcc aggatcatcg
     9361 ccgcgcagcc gacgacccgg ccgcccagcc gggcgaccag gttgacctgg tcgggctggc
     9421 ccgccttccg ccggaaatac tccgcggccc cggatgcgac ggcggcgcgg accgccggga
     9481 ccgcgttcgc ctcgtccatc tccgcgaaca tgcgggcgcg gaactcgccg agcaaccccg
     9541 cctcggccgg ccccgcggct tcgatcgcca ggccctcggg caggctcctg ccgccgccgc
     9601 ccctcgggcc gtccgctccg aggccgcctg ccggggcctg gcggatgagc cccgcggcgg
     9661 ccaaccgctc ctccacgagc tcgacgcatc ttcccacgat gggcttgcag accaggtcgg
     9721 aaagcccctc ggccttgacc ctcgccttgc cctcctcggt cctgaggtcg gcgccccgga
     9781 gcagcgccct gcagtcggag gcgccgaact cggccgccgc cgccaaggcg agctcccggg
     9841 aggcggcgta ggccgcctcc ttcctgtcct gctcctcgcc ccgggcgttc cccagggcca
     9901 ggcccagggc caggacgccc cccgacacgg ccccgcaggt cagctggagc cggccgtagc
     9961 ccgcgcccag gccggaggcg atccgctggg ccgtcccttc gtccaggcca aggtcctccg
    10021 cgaaggccgc gaacaccgcc tgggcgcagg acgagcccga ggcgtggatg gcggccgccc
    10081 tctcggcgcg cgtcatcgca ggcgccctca ggcgtccacc ccgaggacgc ccttaaggac
    10141 gaagccgatg aggaaggaga aggcggagac gcccaggctg atgaaggtca tctccaggaa
    10201 gcgccgcttg aagtcgatgt ccttcgcgac ggccaggtag tagttgaaga ggaagatgat
    10261 gaggacggcc gtggccaggg tgatgccgag ggccaggatc ttggtggaga ggaggaggaa
    10321 gggcaggatg aggaggatga ccgtgaagaa gtaggcgacg cccgtgtaga gcgcggactt
    10381 cgccgcgcgc gggtcgttct ccgccttgga ggagaggtag tcggaggccg ccatggagaa
    10441 ggccgcggag atgcccgtca ccaggcccgc cagggagatg atcttcgtgt ccgccagggc
    10501 cagggtgaag cccgcgaggg cgcccgtgag ctcgaccagg gcgtcgttca ggcccaggac
    10561 cacggagccg acgtactgga gccgctcctc gtccagcatg ccgagaaggg ccttctcgtg
    10621 ggctccctcc tccgcggaga ggcgggccgt ctcggggtag gccgtgccca ggtcggcgta
    10681 gcgcctggag gccgtgccct cgttcctctc catctgcttg aggacgaagg tgaggccgag
    10741 gagcctggcg aggaggacga cgcggaagac gcggaggcgg ttgggcctgg gcgcgacgcc
    10801 cgtcttcgcc gcccagaact cggcgtgctt cttctcctcg gcggcgatgc gcgaaagcac
    10861 ctccgcgttc ttggcgtccc tgcagcccgc ggccaggcgg gagtagatgt ggaattcggt
    10921 gatctcgttg cgctcggcgg cgagcgcggc catcatgatc ttgtcgctca tgccgccgag
    10981 tatagcgccg ccccggcgcg cgcgcgaggg gcggcacgca gggaaggcgc gcgcgagggg
    11041 cggcccgacg gggaccgcgc gcgggggggc gccgccgtag cgccgcggcc gcccgccgcc
    11101 tttacttgta gctccgcgcg gaggcgaccg ccttgtccca ggaggccagg agccgctccc
    11161 gcttctcctc cgccatctcg ggcaggtact cgcgcttccg cctccagttg cgctccacct
    11221 cggccaggct ctcccagtag cccacggaga ggcccgccag gtaggcggcg cccagggcgg
    11281 tgatctcgcc cacctcgggc aggaccacgg agcggtccag gacgtcggcc tggaactgca
    11341 tgaggaagtt gttcgcggag gcgccgccgt cggccttgat gcaggagatg ggccgtccga
    11401 agtccttctc catcgcgacc aggaggtcgc gcgactggta ggcgatggcc tccagggcgg
    11461 cgcggacgaa gtgggccctc gtcgtgcccc ttgtgatgcc gaccacggtg ccgcgcacct
    11521 cggagtccca gtagggcgcg cccatgccga cgaaggccgg caccatgaag acctcgcc
//
LOCUS       DAOOSJ010000056        11244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3822_length_11244_cov_5.615158, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000056 DAOOSJ010000000
VERSION     DAOOSJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_11244_cov_5.615158"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLG14_04310"
     CDS             complement(<1..406)
                     /locus_tag="PLG14_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX48388.1"
                     /translation="MELVYGHPKSLVSVQSKYCPGCGHGVIHRLICEAIDELGVQDRS
                     VIMNPVGCSIWADLYFDFDSVQAPHGRTPAAATGVKRMLPDHLVVCYQGDGDLAAIGT
                     AEIVHAANRGEHFTGIFVNNAIYGMTGGQMAPT"
     gene            complement(424..1488)
                     /gene="vorB"
                     /locus_tag="PLG14_04315"
     CDS             complement(424..1488)
                     /gene="vorB"
                     /locus_tag="PLG14_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOX48389.1"
                     /translation="MADEKRLMKGNEAIGEAAVRAGCRAYFGYPITPQNELTAYMAKH
                     MLDKKRTFIQAESETSAINMVFGAAATGARAMTSSSSPGISLKQEGISYACGADLPMV
                     IVNVARSGPGLGGISPGQADYFQSTRGGGHGDYFHIVLAPKSVQEAADLTYKAFDLAD
                     KWRMPVMILADGLIGQMMEGVVFPPARDPESLPKKAWAAGNRGARKENNHVTSINLVP
                     LELEALVKARFERYAEIKKAEVAYEEVNVDKADLILVGYGTSSRVALGALEMAKAEGL
                     ELGLFRPISLWPFPEKRIAELAAKGKKFLAVEMSMGQMVEDVRLAANGKGEVHFYGRC
                     GGVIPSEEEVLAEAKRLLGK"
     gene            complement(1500..1715)
                     /locus_tag="PLG14_04320"
     CDS             complement(1500..1715)
                     /locus_tag="PLG14_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX48390.1"
                     /translation="MANKGKPEIDRERCKGCHLCIRACPTKVLAPDDEPNSWGYYPAK
                     AVALDKCIACGNCYAVCPDVAITVYKL"
     gene            complement(1734..2630)
                     /locus_tag="PLG14_04325"
     CDS             complement(1734..2630)
                     /locus_tag="PLG14_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOX48391.1"
                     /translation="MKTIAEIIEAAKAGGRKRIAVAAAQEHTALEAAVDAWRHGLAEP
                     VLIGDLKAIDAIAAELKLDISAFERIEEKDFARAAAKAVELVRTGKADMLMKGLLDTS
                     ILLKAAFNKETGINAGRLASHVAVMETAHYHKLFLVTDAAINIAPDLSAKIDIIANAV
                     QVARAIGVETPKVALLAAVEKVNYDKMPCTVDAAVIAQMNRRGQVKGCVVDGPLALDN
                     AVSAESAKIKKIVSEVAGDADILVVPDIEAGNVLYKSLLDLGGAKGAGLVMGAAKPIV
                     LTSRADTAETKLASIALAALAS"
     gene            complement(2641..3708)
                     /gene="buk"
                     /locus_tag="PLG14_04330"
     CDS             complement(2641..3708)
                     /gene="buk"
                     /locus_tag="PLG14_04330"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOX48392.1"
                     /translation="MASTILVINPGSTSTKIGVFEDLKQVFTEGIEHSAEEIGKFPNI
                     AAQYDFRRRHIEAALEKRGFDKKRLSAVVGRGGLLRPIPGGVYAVNEAMKADLSAAKY
                     GEHASNLGALIADALGKELGIPAFIADPVVVDELDEVARVSGHKLFSRISIFHALNQK
                     AVARRWCKENGRKYEESTLIVAHMGGGVSVGLHKDGRVVDVNNALNGEGPFSPERSGT
                     LPAAQLARLCFSGKHTEKEVLKMITGQGGLVSFMGSNDMRVAENAFRAGDPAGTLYYK
                     AFIYQVGKAVGALAAAACGKVDAVILTGGIAYGKEVVHGVAEMCGFIAPVVAYPGEGE
                     LEALALAGSSALSGEAKVQSY"
     gene            complement(3854..5806)
                     /locus_tag="PLG14_04335"
     CDS             complement(3854..5806)
                     /locus_tag="PLG14_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48393.1"
                     /translation="MGRELDVYQLVRSFAHKNALTEIDYRSFADACQRQARQSVTADP
                     LFRDLAINPDTVLIPRLFLVAKDRKLALEMAGNDIRTIVLPERYSDAFFQEYRRIEEN
                     PDVPFPDEESLRLIVPPEWIQTVALDTDLGTVSEKGESPLVPLYRLLFPDNQRPIVVP
                     SAYVPDKLLECAVLKVRQYLRRGGNKDYIQNKLLYAFSAKEIQLKEALGAILTKPFEA
                     IAELQRSSSDFIFPFWAYLTSHLKKDLDKKTDKTAEDLAVCQAASLCEFYANHYKGKA
                     QRLVDLEAAFKTLDQSLRKPPYSYTIEDVLAFRDAKGQPLLGKYSREDLEARLREQTT
                     KGDPSRLPELLVVATGQGRRSYVAKDRLFPLALRLIGEARADLRSRLLDQWRRVLEDF
                     RKSPAMEDDGAYAAELALLVQSRFPLLDALVQEGFLPLVYDELAMRNEAPPDLDHLFY
                     KGALVPMDELLDLPRKVLLSDAKMLLPFWYSVPILAALVRFVRRLGGAERREAGSEKR
                     KSALSAVPKVQAGAAPKAESANGAGAPAKDRRVEFSQAAAAVARELVPSGYSPDEYLR
                     ELEGRWNTLLNPAAKANLTEDVNSLVRDYLRGVLRTMRAGAFTTERVRGLAATLADSP
                     TLLKIKNHAALELYIQLYMVKVLKRP"
     gene            6209..6865
                     /locus_tag="PLG14_04340"
     CDS             6209..6865
                     /locus_tag="PLG14_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX48394.1"
                     /translation="MKRKAGDAEETLFGPGPSPGEPSQAEGLLRGYLRVLALKRLYRQ
                     GWLKRGLPEAACESVADHSFGTALLTLLLAGKPPYEGLDGEKACLMALVHELCEVYAG
                     DLTPDDGLGPAEKRKFERDSLLRVLRGLPDGGRLRGLWEEFEEGSSPEARLVKQLDRL
                     EMGIQAAVYRAEGAPRMEEFLESARFALKDGLLAEILSLAEGKAPSGPQAARPDALSP
                     "
     gene            6944..7546
                     /locus_tag="PLG14_04345"
     CDS             6944..7546
                     /locus_tag="PLG14_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX48395.1"
                     /translation="MNVSFTLSPKRQKREIEIFEAALSVFAEYGFRKAGIEDIAGRLE
                     IAPATLYRYARDKRDLYRKAVEYAFEAWQDAALAAAAEVRDPVGRFRTLCRAAFEHLG
                     REPRLRRILAADPALFPVADAEDPYSSINERSRRLLERTIEEGVEAGAFAAPDPRAAS
                     ILLFSLYRLFIDKAYVEEEADEAALFEKALDLVLDGLRAR"
     gene            7570..7971
                     /locus_tag="PLG14_04350"
     CDS             7570..7971
                     /locus_tag="PLG14_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOX48396.1"
                     /translation="MGELFGYLDADWTAEARKRLAEALDPEKMNGITTSMADIYEACP
                     DGRMRWMFVRCEGGRLASFEVGEGEAPEAEFRILGSYETFAAISRGELGSQKALMTGK
                     LKLKGNMAKALRLAPLADRINKVLSTIPAKY"
     gene            7990..9213
                     /locus_tag="PLG14_04355"
     CDS             7990..9213
                     /locus_tag="PLG14_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOX48397.1"
                     /translation="MSYENDPKSPYYIDNRARVAAIQASMKARGLDLYLGSRPRTLSW
                     VLDAFCPWRSYLAIPRSGDPVVFTFIVDSTRLADETWLPLDNVRAYAPMGGQDQVSVI
                     SAYVTEELGLSGGRIGAETGLSVYTPEGNLSKYEWDALEAALPGFALENAHELVDELQ
                     LVKDPGTMARFREASRMVDEGQKAARAALEGGGWKGMTETEIAGIGALAMRRAGSVSE
                     WNFAGLNEISSGYRTALGACTPPTNRVFQAGEPLMLDFHSMFKLALGDYSHNYLIGPA
                     SKTLRRHADNFVDLVDTVLKAYRAGRTPSDMASVMSARAEALGCADYVYPGCEHGIGL
                     FGDEWRIGARNDGPFPYWTDPDHVYRKDEMLICAMQYAAPADSVGFRYENPIIIGEGG
                     CEWLSKFPLAIEEIA"
     gene            complement(9210..10685)
                     /locus_tag="PLG14_04360"
     CDS             complement(9210..10685)
                     /locus_tag="PLG14_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48398.1"
                     /translation="MAEKRAGVSIGVKAFLASFLILLALMAGAGVLTRVLPAGAFDRV
                     AGPSGPVIDPASYRILPRPDYPVWRWFLAPVEVLGSEDALLVLVIVAFIAVVGGSFAV
                     LQKAGILEAAVRSLASRAGGRKYRLLALVALFFMLIGSLMGIFEEVIPLVPVAIALAA
                     SLGWDLMVGLGMSILATGFGFSAAIANPFSIGTAQRLAGLPVFSGAAFRVLVFAVVYA
                     LYAAWLVGAAKRSERRARAALDSGGAVAQAGGAATQAGAQGAGVGEGGEAGLDPAKVA
                     RGVRFFAGAAVLLLLVVVAISVTQTLSDYSMPITALLFLVAGSGSGLVAGLRPRKALK
                     AFLEGIVGILPGIVLILMAVSVKRIIVTGGIMDTILESARRAVAGSSGYGAAALIYLF
                     TLAMNFFIGSAAAKAFLLIPILAPLGDLAGVSRQVVVQAFAFGDGFSNMLYPTNAVLL
                     ISLGIAGLSWPAWLRRTWKLQAGMLAATMGLLMLAVAIGYR"
     gene            10845..>11244
                     /locus_tag="PLG14_04365"
     CDS             10845..>11244
                     /locus_tag="PLG14_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48399.1"
                     /translation="MAYALFALLAALGTALAAAVLRAIATPRAAPPSPPLPDSALAEA
                     AGVEEKLAAALRRATVSSFDPGKEDGEAFKALKRDLAALFPRVHAELGREELGDRALL
                     FRWEGSDPSLAPAILCAHFDVVPADDAAEWR"
BASE COUNT         1741 a         3715 c         3979 g         1809 t
ORIGIN      
        1 tggtcggggc catctggccg ccggtcatgc cgtagatcgc gttgttcacg aagatgcccg
       61 tgaagtgctc gccgcggttc gcggcgtgga cgatctcggc ggtgccgatg gcggccaggt
      121 cgccgtcgcc ctggtagcag acgaccaggt ggtcggggag catgcgcttc acgccggtcg
      181 ccgcggcggg ggtgcggccg tggggcgcct ggacggagtc gaagtcgaag tagaggtcgg
      241 cccagatcga gcagccgacg gggttcatta tgacggagcg gtcctgtacg cccagctcgt
      301 cgatcgcctc gcagatgagg cgatggatga cgccgtggcc gcagccgggg cagtacttgc
      361 tctggaccga taccaggctc ttggggtggc cgtagacgag ttccatgtcg ctttctcctt
      421 cgatcacttg ccgaggaggc gcttggcctc ggcgaggact tcttcctcgc tcgggatgac
      481 gccgccgcag cggccgtaga agtggacttc gcccttgccg ttggcggcca ggcgcacgtc
      541 ctcgaccatc tggcccatgc tcatctccac ggcgaggaac ttcttcccct tcgcggcgag
      601 ctcggcgatg cgcttctcgg ggaagggcca gaggctgatg gggcggaaga ggccgagctc
      661 gagcccttca gccttcgcca tctccagggc gccgagggcc acgcggctcg aggtgccgta
      721 gccgaccagg atgaggtccg ccttgtcgac gttgacctcc tcgtaggcga cctcggcctt
      781 cttgatctcc gcgtagcgct cgaagcgcgc cttcaccagg gcctcgagct cgagcggcac
      841 gaggttgatg gaggtgacgt ggttgttctc cttgcgcgcg ccgcggttgc cggcggccca
      901 ggccttcttg ggcagggact cggggtcgcg ggcgggcggg aagacgacgc cctccatcat
      961 ctggccgatg aggccgtcgg cgaggatcat gacgggcatg cgccacttgt cggcgaggtc
     1021 gaaggccttg taggtgaggt cggcggcctc ctgcacgctc ttgggcgcga ggacgatgtg
     1081 gaaatagtcg ccgtggccgc cgccgcgggt cgactggaag tagtcggcct ggccggggga
     1141 gatgccgccg aggccggggc ccgagcgcgc gacgttcacg atgaccatcg ggaggtcggc
     1201 gccgcaggcg taggagatgc cctcctgctt gagggagatt cccgggctcg aggagctcgt
     1261 catcgcgcgc gcgccggtcg cggcggcgcc gaagaccatg ttgatcgcgg aggtctcgct
     1321 ctcggcctgg atgaaggtcc gcttcttgtc gagcatgtgc ttcgccatgt aggcggtgag
     1381 ctcgttctgg ggcgtgatcg ggtacccgaa gtaggcgcgg cagccggccc ggacggcggc
     1441 ctcgccgatg gcctcgttgc ccttcatgag gcgcttttcg tcagccatgt cgctcctcct
     1501 tagagcttgt agacggttat cgccacgtcg gggcagaccg cgtagcagtt gccgcaggcg
     1561 atgcacttgt cgagggcgac cgccttcgcg gggtagtagc cccacgaatt cggctcgtca
     1621 tccggcgcga ggaccttggt cggacaggcg cggatgcaga ggtggcagcc cttgcagcgc
     1681 tcccggtcga tctcgggttt gcctttgttg gccatggatc tctccttttc cttctagctc
     1741 gccagcgcgg cgagggctat ggaggctagt ttcgtctccg cggtgtcggc gcggctggtg
     1801 agcacgatcg gcttggcggc gcccatcacg aggcccgccc ccttcgcccc gcccaggtcg
     1861 aggaggctct tgtagagcac gttgcccgcc tcgatgtcgg ggaccacgag gatgtcggcg
     1921 tcgccggcga cctcggacac gatcttcttg atcttggcgc tctccgcgct caccgcgttg
     1981 tccagggcga gcggcccgtc cacgacgcag cccttcacct ggccccggcg gttcatctgc
     2041 gcgatgaccg ccgcgtccac ggtgcagggc atcttgtcgt agttgacctt ctccaccgcg
     2101 gccaggaggg cgaccttggg cgtctccacg ccgatcgcgc gggcgacctg gacggcgttg
     2161 gcgatgatgt cgatcttcgc ggacaggtcg ggggcgatgt tgatcgcggc gtccgtgacc
     2221 aggaagagct tgtggtagtg ggcggtctcc atcacggcga cgtggctcgc gaggcggccg
     2281 gcgttgatgc cggtctcctt gttgaaggcg gccttgagga ggatggaggt gtccaggagg
     2341 cccttcatga gcatgtcggc cttgccggtc ctgacgagct ccacggcctt ggcggccgcg
     2401 cgggcgaagt ccttctcctc gatccgctcg aaggcggaga tgtcgagctt gagctcggcg
     2461 gcgatcgcgt cgatcgcctt caggtcgccg atgaggacgg gctcggccag gccgtgcctc
     2521 cacgcgtcga cggccgcctc cagggcggtg tgctcctggg cggcggcgac ggcgatcctc
     2581 ttgcggccgc ccgccttcgc cgcctcgatg atctcggcta tggtcttcat caggattctc
     2641 ctagtagctc tggaccttgg cctcgcccga gagggcgctc gagcccgcga gcgcgagcgc
     2701 ctcgagctcg ccctcgccgg ggtaggcgac gacgggggcg atgaagccgc acatctcggc
     2761 gacgccgtgg acgacttcct tcccgtaggc gatgccgccc gtgaggatga ccgcgtccac
     2821 cttcccgcag gcggcggccg cgagggcgcc gaccgccttg ccgacctggt agatgaaggc
     2881 cttgtagtag agggtcccgg cggggtcgcc cgcgcggaag gcgttctccg ccacgcgcat
     2941 gtcgttcgag cccatgaagg acacgaggcc tccctgtccg gttatcatct tcaggacctc
     3001 cttctccgtg tgcttgccgg agaagcagag cctggcgagc tgggcggcgg gcagggtgcc
     3061 cgagcgctcg gggctgaagg gaccctcgcc gttcaatgcg ttgttgacgt cgacgacgcg
     3121 gccgtccttg tggaggccga cggagacgcc gccgcccatg tgggcgacga tgagcgtgga
     3181 ctcctcgtac ttccggccgt tctccttgca ccagcgcctg gcgacggcct tctggttcag
     3241 ggcgtggaag atggagatcc gggagaagag cttgtggccg gagacgcggg ccacctcgtc
     3301 gagctcgtcg acgacgacgg gatcggcgat gaaggcgggg atcccgagct ccttgccgag
     3361 ggcgtcggcg atgagggcgc cgaggttgga ggcgtgctcg ccgtatttcg cggcggagag
     3421 gtcggccttc atcgcctcgt tgacggcgta gacgccgccg gggatgggcc ggaggaggcc
     3481 gccgcggccg acgacggccg aaagccgctt cttgtcgaag ccgcgcttct ccagggccgc
     3541 ctcgatgtgc ctccggcgga agtcgtactg cgcggcgatg ttcgggaact tgccgatctc
     3601 ctcggccgag tgctcgatgc cctcggtgaa gacctgcttc aggtcctcga agacgccgat
     3661 cttcgtcgac gtggaaccgg gattgatgac gagaatcgtg gaagccatga ctgctccttg
     3721 ccctatgggc ttggggactt cgctttcagc cgagtatatc atgttttgga gaaagcgcaa
     3781 ccagaaaaca cacctatcaa aaggtcgttt cgtcaaacga aacgccccag agtggtttag
     3841 ctggcaaaac gaactaaggg cgcttgagca ccttgaccat gtaaagctgg atgtagagtt
     3901 cgagggcggc gtggttcttg atcttgagga gggtcgggga atcggccagg gtggcggcga
     3961 gaccgcgcac ccgctccgtc gtgaaggcgc cggcccgcat ggtgcgcagg acgccgcgga
     4021 ggtagtcgcg gaccaggctg ttcacgtcct ccgtgaggtt ggccttggct gcggggttca
     4081 agagggtgtt ccagcgccct tcgagctccc gcaggtactc gtcggggctg tagccggagg
     4141 ggacgagctc gcgggccacg gcggcggcgg cctgggagaa ctcgacccgc ctgtccttgg
     4201 ccggggcgcc ggccccgttc gcggactcgg ccttgggcgc ggcccccgcc tggaccttcg
     4261 ggaccgcgct cagggcggac ttccgcttct cggatccggc ctcccggcgc tcggcgccgc
     4321 ccaggcggcg gacgaagcgg accagggccg cgaggatggg caccgagtac cagaaaggca
     4381 ggagcatctt cgcgtcggag aggaggacct tcctcggaag gtccaggagc tcgtccatgg
     4441 ggacgagggc acccttgtag aagaggtggt ccaggtcggg cggagcctcg ttccgcatcg
     4501 cgagctcgtc gtagacgagg gggaggaagc cctcctggac cagggcgtcg aggaggggga
     4561 agcgcgactg gacgaggagg gcgagctccg cggcgtaggc cccgtcgtcc tccatggccg
     4621 gactcttgcg gaagtcctcg aggacgcggc gccactggtc caggaggcgg ctccgcaggt
     4681 cggcgcgggc ctcgccgatg agccgcaggg ccaggggaaa gaggcggtcc ttcgcgacgt
     4741 agctccgccg gccctggccg gtcgcgacga cgagaagctc ggggaggcgg gagggatcgc
     4801 ccttggtcgt ctgctccctg agccgcgcct ccaggtcctc cctcgagtac ttgccgagga
     4861 ggggctggcc cttggcgtcg cggaaggcca gcacatcctc gatggtgtag ctgtagggag
     4921 gcttccggag ggactggtcg agggtcttga aggccgcctc caagtcgacg aggcgctggg
     4981 ccttgccctt gtagtggttc gcgtagaatt cgcagagcga ggcggcctgg cagacggcca
     5041 ggtcctcggc cgtcttgtcc gtcttcttgt ccaggtcctt cttgaggtgg ctcgtgaggt
     5101 aggcccagaa ggggaagatg aagtcgctgg aggagcgctg gagctcggct atggcctcga
     5161 agggcttcgt gaggatggcg cccagggcct ccttgagctg tatctccttg gcggagaagg
     5221 cgtaaaggag cttgttctgg atgtagtcct tgttgccgcc ccggcgcagg tactgcctga
     5281 ccttgaggac ggcgcactcc aggagcttgt cgggcacgta ggcggagggg acgacgatgg
     5341 gccgctggtt gtcggggaag agcaggcggt agagggggac gaggggggac tcccccttct
     5401 ccgaaaccgt gcccaggtcg gtgtccaggg cgacggtctg gatccactca ggcgggacga
     5461 tgagcctcag ggactcctcg tcgggaaagg gcacgtcggg attttcctcg atccggcggt
     5521 actcctggaa aaaagcgtcg gaatagcgct cgggtaggac gatggtcctg atgtcgttcc
     5581 cggccatctc cagggccagc ttgcggtcct tcgcgaccag gaagaggcgg gggatgagca
     5641 cggtgtcggg gttgatggcc aggtcgcgga agagcggatc ggcggtgacg gactgcctgg
     5701 cctggcgctg gcaggcgtcg gcgaaggagc gatagtcgat ctcggtgagg gcgttcttgt
     5761 gcgcgaagga gcgcaccagc tgatagacgt ccagttcccg gcccataggc caccaatggt
     5821 agcctaaagc cccggcctag gcaagcgacc aaatgaaacg gccgcccaca gaggggcggc
     5881 cattcgcgtt ccgaagatcg cggcgctact ttcgggtctt gtgccgattg tttctcttct
     5941 tcttcttgcg cttgtgcgtc gctatcttct tgagctttct ctttcgtccg cagggcactt
     6001 tagcctctcc ttcgcgtagc gatccggaaa agtacggagt ctcaagtcct ccgcatgaat
     6061 tcgcttcatt tattaaacca tagcccccct ccctcgtcaa gaggtcgagg cgccggcttg
     6121 ggatcgccgg gacgccaggc cccccgtggg ctgtcgaggc ccgaccgcgt ccttgccccc
     6181 gcccggcctc cctgctaggc tcaaagccat gaagcgaaaa gccggcgacg cggaagagac
     6241 gctgttcggg ccggggccga gccccggcga acccagccag gccgaggggc tgctccgcgg
     6301 ctatctccgc gtcctcgccc tgaagcgcct gtaccgccag ggctggctca agcgcggcct
     6361 tcccgaggcg gcctgcgagt ccgtcgcgga ccacagcttc ggcacggcgc tcctcaccct
     6421 cctcctcgcg ggaaagccgc cctacgaagg gctcgacggg gagaaggcct gcctcatggc
     6481 ccttgtccac gagctctgcg aggtctacgc gggcgacctc acgcccgacg acggccttgg
     6541 gccggcggag aagcggaagt tcgagcggga ctcccttctc cgcgtcctca ggggcttgcc
     6601 ggacggcgga cgcctgcgag ggctctggga ggagttcgag gagggctcga gccccgaggc
     6661 caggctcgtc aagcagctcg accgcctgga gatgggcatc caggcggcgg tctaccgggc
     6721 ggagggagcg ccgaggatgg aggaattcct ggagagcgcg aggttcgccc tcaaggacgg
     6781 cctcctcgca gagatcctct cgctcgccga gggaaaggct ccgtccggcc cccaagccgc
     6841 tcgccctgac gcgctctccc cctagcctgc ccgctcggcc catcctacgg acttgagcca
     6901 aaaaaacgct tgacgggacc gccactcatg ttaaagtgaa tatatgaatg tttctttcac
     6961 tttatctcct aaacggcaga agcgggagat cgagatattc gaggccgcgc tctcggtctt
     7021 cgcggagtac ggcttcagga aggcgggcat cgaggacata gcggggcggc tcgagatcgc
     7081 ccccgcgacc ctctaccgct acgcccgcga caagcgggat ctctacagga aggccgtaga
     7141 gtatgccttc gaggcctggc aggacgcggc cctcgccgcg gcggcggagg tccgggatcc
     7201 ggtcggccgg ttcaggaccc tctgcagggc cgccttcgag cacctgggca gagagccccg
     7261 gctccggagg atcctcgcgg ccgaccccgc cctcttcccg gtcgccgacg cggaggatcc
     7321 ctactcctcg atcaacgagc gctcgcggcg tcttctggag cgcaccatcg aggaaggcgt
     7381 ggaggcggga gccttcgcgg cgcccgaccc cagggcggcc tccatcctcc tcttctcgct
     7441 ctaccgcctc ttcatcgaca aggcctacgt ggaagaggag gccgacgagg ccgccctctt
     7501 cgagaaggcc ctcgacctcg tgctcgacgg gcttcgcgcc cgataatccc tttcccggag
     7561 gcgacgacca tgggagaact cttcggctac ctggacgcgg actggaccgc agaggcgagg
     7621 aaacgcctcg ccgaggccct cgatcccgag aagatgaacg gcatcacgac ttcgatggcc
     7681 gacatctacg aggcctgccc cgacggcagg atgcgctgga tgttcgtccg ctgcgagggc
     7741 ggcaggctcg cgagcttcga ggtcggcgag ggcgaggcgc ccgaggcgga gttccgcatc
     7801 ctcgggagct acgagacctt cgcggccatc tcccgcggcg agctcggctc gcagaaggcc
     7861 ctcatgaccg ggaagctcaa gctcaagggc aacatggcca aggcccttcg gctcgccccc
     7921 ctcgcggacc ggatcaacaa ggtcctttcg accatccccg cgaagtactg aggaaggagg
     7981 agtcaagcca tgtcctacga gaacgatcca aagagcccct actacatcga caaccgcgcc
     8041 cgcgtcgccg cgatccaggc ctccatgaag gcccggggcc tcgacctcta cctgggcagc
     8101 cgtccgcgga ccctgtcctg ggtcctggac gccttctgcc cctggaggtc ctacctcgcc
     8161 atcccccggt cgggcgatcc cgtcgtcttc accttcatcg tcgactcgac gcggctcgcc
     8221 gacgagacct ggctgcccct cgacaacgtg agggcctacg ctcccatggg cggacaggac
     8281 caggtctccg tcatctcggc ctacgtcacg gaggagctcg gcctctcagg cgggcggatc
     8341 ggcgcggaga ccggcctctc cgtctacacg ccggagggca acctctccaa gtacgagtgg
     8401 gacgccctgg aggcggccct cccgggcttc gccctggaga acgcccacga gctcgtcgac
     8461 gagctccagc tcgtcaagga cccaggcacg atggcgcgct tccgggaggc ctcgcgcatg
     8521 gtggacgagg ggcagaaggc ggcccgcgcg gcgctcgagg ggggcggctg gaagggcatg
     8581 accgagaccg agatcgccgg cataggcgcc ctcgcgatgc gccgggcggg ctccgtctcc
     8641 gaatggaact tcgcgggcct caacgagatc tcctcgggct accgcacggc cctgggcgcc
     8701 tgcacgccgc ccacgaaccg cgtcttccag gcgggcgagc ccctcatgct cgacttccac
     8761 tcgatgttca agctggccct gggcgactat tcgcacaact acctcatcgg cccggcctcg
     8821 aagaccctgc gacgccacgc ggacaacttc gtcgacctcg tcgacacggt gctcaaggcc
     8881 taccgcgccg gccggacgcc ctcggacatg gcctccgtca tgtcggcccg cgcggaggcc
     8941 ctgggctgcg ccgactacgt ctaccccggc tgcgagcacg gcatcgggct cttcggggac
     9001 gagtggcgca tcggggctag gaacgacggg cccttcccct actggaccga cccggaccac
     9061 gtctaccgga aggacgagat gctcatctgc gcgatgcagt acgcggctcc ggccgattcg
     9121 gtcggcttcc gctacgagaa tcccatcatc atcggggagg gcggctgcga gtggctctcc
     9181 aagttccccc tggcgatcga ggagatcgcc tagcggtagc cgatcgcgac ggcgagcatg
     9241 aggaggccca tcgtcgcggc cagcatcccg gcctggagct tccaggtccg cctgagccag
     9301 gcgggccagg acaggccggc gatgccgagg ctgatgagga ggacggcgtt cgtcgggtag
     9361 agcatgttgg agaagccgtc gccgaaggcg aaggcctgga cgaccacctg ccgcgacacg
     9421 cccgcgaggt ccccgagcgg ggcgaggatc gggatgagga ggaaggcctt cgccgccgcc
     9481 gagccgatga agaaattcat cgccagggtg aagaggtaga tgagggcggc ggccccgtag
     9541 cccgaggagc ccgcgacggc gcggcgcgcc gattccagga tggtgtccat gatgccgccc
     9601 gtgacgatga tccgcttcac gctcacggcc atgaggatga ggacgatgcc gggaaggatg
     9661 cctacgatgc cctccaggaa ggccttcagg gccttcctgg gccgcagtcc cgcgacgagg
     9721 ccggagcccg agcccgcgac gaggaagagg agggccgtga tcggcatcga gtagtccgag
     9781 agggtctgcg tgaccgagat ggccacgacg acgaggagga ggaggacggc ggccccggcg
     9841 aagaagcgca cgcctctcgc caccttcgcg ggatcgaggc cggcttcgcc tccctcgcct
     9901 acgcccgctc cctgcgcgcc cgcctgggtc gcggcgccgc ccgcctgggc cacggcgccg
     9961 cccgagtcga gggccgcccg ggcccgccgc tcggagcgct tggccgcccc gaccagccag
    10021 gccgcgtaga gggcgtagac gaccgcgaag acgaggacgc ggaaggccgc gcccgagaac
    10081 acggggaggc cggccaggcg ctgggccgtg ccgatggaga agggattggc gatggcggcg
    10141 gaaaagccga agcccgtcgc gaggatgctc atccccaggc ccaccatgag gtcccagccg
    10201 agcgaggccg ccagggcgat ggcgacgggc acgaggggga tgacctcctc gaagatgccc
    10261 atgagcgagc ctatgagcat aaagaagagg gccacgaggg cgaggaggcg gtacttgcgg
    10321 ccgcccgccc gcgacgcgag cgacctcacg gccgcttcca ggatgcccgc cttctggagg
    10381 acggcgaagg agcctcccac gacggcgatg aaggccacga tgaccagcac cagcagggcg
    10441 tcctcggagc ccaggacctc cacgggagcc aggaaccagc gccagacggg atagtcgggg
    10501 cgcggcagta ttcggtagct agccgggtcg atgacgggcc ccgaggggcc cgcgacgcgg
    10561 tcgaaggccc cggcgggcag gactcgcgtg aggacgcccg cgccggccat gagggcgagg
    10621 aggatgagga agctcgcgag gaaggccttg actccgatgg acacgccggc gcgcttttcc
    10681 gccatggatc gctccttgcg cccctattgc aggaccgggg cgcgacgccc agtatagccg
    10741 ccgcgcgcgg cgcctgtcga acaaaatcgc ccatcgcgcc gcttgcgccc gttgaccagg
    10801 ccgcgccccg ggcggatact ggtagcccgc aaggagtcat cgcaatggcc tacgcgctct
    10861 tcgccctgct cgcagccctc gggaccgccc tcgcggcggc cgtcctccgc gccatcgcca
    10921 cgcccagggc ggcgccgccc tcccctcccc ttcccgactc ggccctggcg gaggccgccg
    10981 gcgtggagga gaagctggcc gccgcgctgc gccgcgccac cgtctcctcc ttcgatcccg
    11041 ggaaggagga cggcgaggcc ttcaaggccc tgaagcgcga cctcgccgcc ctcttccccc
    11101 gcgtccacgc ggagctcggc cgggaggagc tcggcgaccg ggccctcctc ttccgctggg
    11161 agggaagcga tccctccctc gcgcccgcca tcctctgcgc ccacttcgac gtcgtccccg
    11221 ccgacgacgc cgcggaatgg cgcc
//
LOCUS       DAOOSJ010000057        11238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3825_length_11238_cov_6.106054, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000057 DAOOSJ010000000
VERSION     DAOOSJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_11238_cov_6.106054"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..2125
                     /locus_tag="PLG14_04370"
     CDS             164..2125
                     /locus_tag="PLG14_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48400.1"
                     /translation="MRSPLLPPRSGLLAILLSLLVLEAGAAPSAPAKPAAPAAPAKPA
                     APAATKPAAAPAKLPGLPGLLPAESAKKAALLLEAIDAANKGIEARLDAARKAGGAAE
                     LAALEAAAVLEASQGGLSAISPDASYSGAEAAAAALAAARTKAFAAALAVAEGKAMAE
                     GKSVAELSALRAAKAKELAALVAAAGLGEKAALAAEPELLRRSKGLPELFPEGAAAGK
                     ALMKAGAAGRKIWLSALSACDAEAAAEALVAARPEAAKAAPAASSALDGLEAALRAFR
                     AWRTAAPFALHPSAFMEGGQEGAPGTAPLNAAYQALGALGEERAVALLKAFEASPYRV
                     SEASAAQRLSLALEAMTVERRRGAALGLGLSPADCLGLALLCGRAAAPAAKEPPAAKE
                     PAAAKEPAAAKEPAAAGTAPLAPAAAAGKAAGQATSAHEAAALEASVRELNRLKAELA
                     AAEGAPDRGGRERTIALALLARRDLLALAAQDERYAALFSAAGKSLGPVFREAEKGAL
                     ERAARDPAAAKRLARLKALHKAEPRLSLAEGKPEAGVRRFSLLARFPAAEGAEAVAAM
                     DPALAGPAYALAFAEAAGLASELKSLGPAAFLERYGVRFVPAYPGARDLVALPASAFP
                     PPPKGADAALAAARLAAAFEAGLCAEATR"
     gene            2122..3990
                     /locus_tag="PLG14_04375"
     CDS             2122..3990
                     /locus_tag="PLG14_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOX48401.1"
                     /translation="MRQRRGFSLGFSGGALAGAARALLSALLLSILLAAALSACGGKP
                     GGPDEEADSAKARALEAEKAAAQPDPRLAYASSRLLSGLPDPLGEPMVLAAPRGLPRL
                     GPVASKDGRYLAYALAGPERLVLVDTVSGTAAAETRLGSPAVALAFLEAPGALLVAAC
                     LDGSLAAYDEGLAESWRRSGGPASALVTLPEKRLAVVEEGGTVHLLPAEGGEPLWRAE
                     AGGRIFAAAYSPGALCLLAAAPQSPAAAPEAPTASGPAAAVSPPPAAPEAAIASGPAA
                     SPPAASPPAAAPTAASPSSGTASPAASPTAAGASSYLWRLFALSESDGRELWSRELES
                     PAAHLSADGSRLALVTLSGGIELREAKDGILVARGAGRFDPGVPPILAGSSVFAALSG
                     GGPAELDAATGAPKPSPAYPPEVRGGAISTLAADEDRVFAARGGSLAAWSRHSGSEPR
                     MAELPIAPAASPVSAGGRLWLLGPGGELLSLGGPASDGQALRSPLDALAPPGETAEAI
                     ASSLEKFKPRNGLPLDRYLRFDLFADGLPIGGEVPFVALRRSAGKAEKGLLYAAPEGG
                     RILLAIFSESGEELSANVDELGAEARHEARFDEGKTYWLLTGRLDAEGGAYRIFYR"
     gene            complement(4148..5422)
                     /locus_tag="PLG14_04380"
     CDS             complement(4148..5422)
                     /locus_tag="PLG14_04380"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOX48402.1"
                     /translation="MPKVLSIVLGGGKGTRLYPLTKDRAKPAVPFGGKYRIVDIPISN
                     CINSGFRQIYILTQFNSASLHLHLARTYSFDSFSRGFVEILAAEQTLVHSGWYEGTAD
                     AVRKNLMHFRPQNPSHYLIVSGDQLYRMDLADMFKRHVESGCGLTIACTSVSREDASD
                     LGIAKVDAKSRIVEFMEKPGPTKDVSAYKIPAELLDDRRTGGKDYLASMGIYIFDAAL
                     MEKSLEGDQNDFGKEIIPSILAKMPVNAYVFNGYWEDIGTIKSFYEANLDLASINPQF
                     NFYDEERPIYTHKRNLPASKLNYSTLNLSLTADGCIITNAGLNHSIVGVRTIIESGAS
                     LDGVVCMGADYYESEAQKEANAKGGVPNIGIGRGSIIKGAIIDKNARIGENCRIGIDL
                     MDRPEGEYPTHHVVDGIIVIPKNTVIPSGTSI"
     gene            5488..7863
                     /locus_tag="PLG14_04385"
     CDS             5488..7863
                     /locus_tag="PLG14_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX48403.1"
                     /translation="MIDASARTFIDEILADPDVAPRIAHVESAPARDADWAPLPDGLD
                     PRLLEALKAKGYERLYSHQRASYDAIRAGENVVVVTPTASGKTLCYNLPVVQTLLEEP
                     EARALYLFPTKALSQDQQAELNEVALGGSLPVGIYTYDGDTPSSLRVAARDSGRIVIT
                     NPDMLHSGVLPNHAKWIRFFAGLRYVVVDEAHAYRGVFGSHVANVIRRLKRVAAFYGA
                     HPRFVLCSATIGNPEELASALVEEPVRALSENGAPRGERRVVFYNPPLLDPVQGIRKS
                     TATESEKLALLLLRRGVKTILFARSRLKVELIASYISKALENLYTGNGGVRVSPYRSG
                     LLPSERRAIEKGLREGSIQGVVSTNALELGIDIGGLDAAVVAGFPGSFASFWQQAGRA
                     GRRGSVSVAFLVASSSPLDQFILAHPEYFFGRSPESARIDPDNPYIFADHAKCAAFEL
                     PFKEGESLGKAGPEATAEVLSMLEEEGLVRLTGGRWYWSAAGYPSEAISLRSATADNV
                     VIVDRTGGGARVIGEMDRPSAKELIFDDAVYIHLGRQYKVRKLDLANKTCFVEEKEVD
                     YWTDSIVKTDLEVLTEDEEGLLAAQGGRGPLASWVLGDVLVRTEAEKYKKLRFHTHEN
                     VGYGEISLPPEEMQTRSLVLLLPPGSAGGDLVAACPEGGKAALLAGAARLIHDLAPVF
                     LMCDGRDLGLAERVRDPHFGVPAIYFYDRYPGGTGLAEGLAELLGPAIRAARERLASC
                     GCEEGCPSCVGVDFGEAAGPQAARGGRAVKASVLELLSALSEAAPGDGQEP"
     gene            7847..9298
                     /locus_tag="PLG14_04390"
     CDS             7847..9298
                     /locus_tag="PLG14_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOX48404.1"
                     /translation="MAKNLKGRLARLRELGLVKAAELEAPGAGEKGAFSGGAAEGEAA
                     AQGGAPVGEGGSTSPSARRRPGAGARGARAGGGERPGVPSFLAGWTQAAELVWERELR
                     FPSHLPPLIDPRPFAAMSRKAARGSLDAAKPGVRSAAKPGARAADGAAPLGAGGRPEA
                     ATRGAGLAGGADGLLPSESLRFFDLETTGLSGGSGTVAFLAAVGRIEAGEFAVRQLFL
                     ADYPGERAWLEALLSLLSEGSVVTTYNGRAFDLPLLRTRLVMNGMRPPSFAEIDALFV
                     ARRLWRRVHGGASLSLLERLVLDLERELDVPGSAIPDIWFSYLREGDHPLMAAAMAHN
                     AEDVSGLERLAARAARIFEEPRAWAASGAVDRAGLGRGLLALGRADEGEELLEAALGE
                     GDAAAGLLLSRRYRREGRLEDRRRVVGMLPEGLESCVERAKFHEHALKDFRAALAWTD
                     RAISWAREGGGTEAFEALEARRARLLRKLERGG"
     gene            complement(9412..10434)
                     /gene="rlmN"
                     /locus_tag="PLG14_04395"
     CDS             complement(9412..10434)
                     /gene="rlmN"
                     /locus_tag="PLG14_04395"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOX48405.1"
                     /translation="MELSLTGMGPAEVAAACGFKAAFRGRQVYRWLARGASSFEEMSD
                     LPKPERERLSALFPRIYSSEAAAALEDPDGSLKLQLRLRDGAAVECVLLEDIEGRKTA
                     CLSSQVGCPMACAFCKTGTLGYLRDLGPDEILEQYHELKRRRGDISNIVFMGMGEPLL
                     NLAAVRKAVSILSDPKGIGLSPRRITISTCGIVPGILDLAEHGPKLRLAISLTAADDD
                     LRSELMPVNRKWGLAELKAALLKYQEATGERVTLEAALMGGRNSRPEDARALIRWIRP
                     LKAQVNLIPWNRVPGLPFVEPTRDEVETFQAILDEAGLTSTRRMRRGRGVMGACGQLG
                     DTLLSK"
     gene            complement(10435..>11238)
                     /locus_tag="PLG14_04400"
     CDS             complement(10435..>11238)
                     /locus_tag="PLG14_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48406.1"
                     /translation="AIEIWEQYLLHDNKNITVLTRVADAYRKVRDFRKSKAIYLRVLE
                     MEQDNPYALIGLGHLHYDFKEYKDALGYWQRMVELQGEGVDIRVLTSIGNCYRKLKQF
                     DLGVPYFERALGKEPDNFYALFGLADCYRGSGQQHRSLDYWKQILAKDPRNKVILTRG
                     GDAYRSMGEYDKAAEFYERALNIEFDVYAVLGLAVIARMQGKYQDAVTSLRKLVQNDP
                     KNYRLYLELSQCYVGLGDKQRALEALVDFQRLGIRNVYVQDAVAKLQRA"
BASE COUNT         1471 a         4042 c         4095 g         1630 t
ORIGIN      
        1 agggcgggct cggcctgcgt cgacggggcc ctgcccctcg agggcttcga ggccgatttc
       61 cgaggcggct accgcccagg cccggcgggt ccgggcgctc cggacatcgg ggccgacgaa
      121 ttccgggaat gagcccgacc ggcgcgatag agttgtgatg gccatgcgaa gtccccttct
      181 cccgccgaga tccggcctcc tagcgatcct cctctccctt ctcgtcctgg aggccggggc
      241 cgcgccgagc gctccggcca agcctgccgc gcccgcggcg ccggccaagc cggcggctcc
      301 ggcggcgacc aagccggccg ccgcgcccgc gaagctcccg gggctgcccg gcctccttcc
      361 cgcggaatcg gcgaagaagg cggccctcct cctggaggcg atcgacgcgg ccaacaaggg
      421 gatagaggcc cgcctggacg cggcgaggaa ggcgggcggg gcggccgagc tcgccgcctt
      481 ggaggcggca gccgtcctcg aggcctccca aggcggcctt tcggccattt cccccgacgc
      541 gtcctactcg ggagccgagg cggccgccgc ggccctggcc gccgcgagaa ccaaggcctt
      601 cgccgcggcc ctcgccgtcg ccgagggcaa ggccatggcc gaggggaaga gcgtcgccga
      661 gctatccgcc ctgcgcgcag cgaaggcgaa ggaactcgcc gccctcgtcg cggcggccgg
      721 cctgggagag aaggccgcct tggccgccga gcccgagctc ctcaggcgct cgaagggcct
      781 gcccgagctc ttccccgaag gcgcggcggc ggggaaggcc ctcatgaagg ccggagccgc
      841 cggacggaag atctggctgt cggccctctc ggcctgcgac gccgaggccg ccgccgaagc
      901 cctcgtggcc gcgaggcccg aagccgccaa ggccgctccc gcggcctcga gcgccctgga
      961 cgggctcgaa gccgccctcc gcgccttccg cgcctggcgc acggccgcgc ccttcgccct
     1021 ccatccctcg gccttcatgg aaggcggcca ggaaggcgcg ccgggaacgg cgccgctcaa
     1081 cgcggcctac caggccctcg gggcgctggg cgaggagagg gccgtcgccc tcctcaaggc
     1141 cttcgaggcg agtccctatc gagtctcgga agcgagcgcc gcccaaagac tgtccctcgc
     1201 tctcgaggcg atgacggtcg agcgccgccg cggagcggct ctcggcctgg gcctgtcgcc
     1261 ggccgattgc ctcggcctgg cccttctctg cggacgcgcc gcggccccgg ccgcgaagga
     1321 gccgcccgcc gcgaaggagc cggccgccgc gaaggagccg gccgccgcga aggagccggc
     1381 cgccgccggg accgcgcccc tggcccccgc ggcggcggcg ggcaaggcgg cgggccaggc
     1441 gacctcggcg cacgaggcgg cggccctcga ggcctccgtc cgcgagctca accgcctgaa
     1501 ggccgagctc gcggccgccg agggcgcgcc cgaccgaggc gggcgggaga ggacgatagc
     1561 cctcgccctc ctcgcccgac gcgatctcct ggccctggcg gcgcaggacg aacgttatgc
     1621 ggccttgttc tccgcggcgg gcaagagcct ggggccggtc ttccgggagg cggagaaggg
     1681 agccctggag cgcgccgcga gggacccggc ggccgccaag aggctcgcga ggctcaaggc
     1741 cctacacaag gccgagcccc gcctttccct cgcggagggc aagcccgagg cgggggtccg
     1801 gcgcttctcc ctcctcgccc gcttcccggc ggcggagggc gcagaggccg tcgcggccat
     1861 ggatccggcc ctggccggcc cggcctacgc cttggccttc gccgaggccg cgggactcgc
     1921 gtccgagctc aagtccctgg ggcccgccgc cttcctcgag cgctacggcg tccgcttcgt
     1981 tccggcctat ccgggcgccc gcgacctcgt ggcgcttccc gcctcggcct ttccgcctcc
     2041 gcccaagggc gcggacgccg ccctggcggc ggcccggctc gccgcggcct tcgaggccgg
     2101 gctttgcgcg gaggcgacgc gatgaggcag cgccgcggat tctccctcgg gttctctggc
     2161 ggagccctcg ccggcgccgc gcgcgcgctc ctgtccgcgc tcctcctctc catcctcctc
     2221 gccgcggccc tctcggcctg cggcggcaag ccgggcggcc ccgacgagga ggctgattcc
     2281 gccaaggccc gcgccctgga ggccgagaag gccgcggccc agcccgatcc ccgcctggcc
     2341 tacgcctcct cgcggctcct ctcgggactg cccgacccgc tcggcgagcc catggtcttg
     2401 gccgcgccgc ggggccttcc gaggctcggc cctgtggcct cgaaggacgg acgctatctc
     2461 gcctacgcct tggccggccc cgagcgcctc gtcctcgtcg acacggtttc ggggacagcc
     2521 gcggcggaga ccaggctggg ttcgcccgcc gtggcgctcg ccttcctcga agccccaggc
     2581 gccctcctcg tcgcggcttg cctggacgga tccctcgccg cctacgacga agggctcgcg
     2641 gagtcttgga gaagaagcgg aggcccggcc tcggccctcg tcaccctgcc ggaaaagcgg
     2701 ctggccgtcg tcgaggaggg cgggaccgtc cacctccttc ccgccgaggg cggcgagccg
     2761 ctctggcgcg ccgaggcagg aggccgaatc ttcgccgccg cctactcgcc cggcgcactc
     2821 tgcctccttg ccgcggctcc gcaaagcccg gccgcggcgc cagaagcacc gaccgcttcc
     2881 ggcccggccg ccgccgtctc gccgcccccc gcggcgccag aagccgcgat tgcttccggc
     2941 ccggccgcat cgccgccggc cgcatcgccg ccggccgcgg cgccgacggc cgcctcgccg
     3001 agttcgggta cggcgtcgcc ggccgcatcg ccgaccgccg ccggagcctc ttcttacctc
     3061 tggcgactct tcgcgctctc ggagtccgac ggacgcgaac tgtggagcag ggagctcgag
     3121 tcgccggcgg cgcatctctc ggccgacggc tcccgcctcg ccctggtcac cctgtcgggc
     3181 ggcatcgagc tcagggaggc caaggacggg attctcgtcg cccgcggcgc cggccgcttc
     3241 gatccgggag ttccgcccat cctcgctggg agctcggttt tcgcggccct gtcgggcggc
     3301 ggcccggcgg agctcgacgc cgcgacgggc gcgccgaagc cgagtcctgc ctatccccct
     3361 gaagttcggg gcggcgcgat ctcgaccctg gccgcggacg aggacagggt cttcgcggcc
     3421 aggggcggga gcctcgcggc ctggtccagg cactcaggct ccgagccccg gatggccgag
     3481 ctgccgatcg ccccggcggc ttccccggtc tcggccggag gaaggctctg gctgctgggc
     3541 ccgggcggcg aactcctctc cctcggaggg ccggcctcgg acgggcaggc cctccggtcg
     3601 ccgctcgacg ccctcgcgcc ccccggcgag acggccgagg ccatcgcctc gagcctcgag
     3661 aaattcaagc cgcggaacgg gctccccctg gaccgctacc tgcgcttcga cctcttcgcc
     3721 gacggcctgc ccatcggcgg ggaagtccct ttcgtggcgc tcaggcggag cgcgggcaag
     3781 gctgagaagg gtcttctcta cgcggcgccg gagggaggca ggatcctcct cgcgatcttc
     3841 tcggagtccg gcgaggagct ttccgccaac gtcgacgaac tcggggcgga agcccggcac
     3901 gaggcgcgct tcgacgaggg caagacctac tggctgctca cgggaaggct cgacgccgag
     3961 ggcggcgcat acaggatctt ctaccgctga gggcccggcg cccggaccgc cgccgccggc
     4021 gacgcctcgc agcccgccgc gcgcggcggg gccacatcgt gggcggccac cgcgcgcggc
     4081 gagcccgacg caaagcaaga ggccgcccct ttcggggcgg cctcgcctgt ctagagccgg
     4141 cgcccggcta gatggaggtc ccgctcggga tgaccgtgtt cttcgggatc acgatgatcc
     4201 cgtcgaccac gtggtgggtc gggtactccc cctcggggcg gtccatcagg tcgatgccga
     4261 tgcggcagtt ctcgccgatc cgcgcgttct tgtcgatgat ggcgcccttg atgatcgagc
     4321 ccctgccgat gccgatgttc ggcacgccgc ccttcgcgtt ggcctccttc tgcgcctcgc
     4381 tctcgtagta gtcggcgccc atgcagacga cgccgtccag gctcgcgccc gactcgatga
     4441 tcgtgcgcac gccgacgatc gagtggttga ggccggcgtt cgtgatgatg cagccgtcgg
     4501 cggtcaggga gaggttcagg gtcgagtagt tgagcttcga ggcgggaagg ttgcgcttgt
     4561 gcgtgtagat cggcctttcc tcgtcgtaga agttgaactg gggattgatg gaggccaggt
     4621 ccaggttggc ctcgtagaag gacttgatgg tgccgatgtc ctcccagtag ccgttgaaga
     4681 cgtaggcgtt gaccggcatc ttcgcgagga tggaggggat gatctccttg ccgaagtcat
     4741 tctggtcgcc ctccagggac ttctccatga gggcggcgtc gaagatgtag atgcccatgc
     4801 tcgccaggta gtccttgccg cccgtcctcc ggtcgtccag gagctccgcc gggatcttgt
     4861 aggcggagac gtccttcgtc gggcccggct tctccatgaa ctcgacgatg cggctcttgg
     4921 cgtcgacctt ggcgatgccg aggtcgctcg cgtcctcccg ggagacggag gtgcaggcta
     4981 tcgtgaggcc gcagccggac tcgacgtggc gcttgaacat gtcggccagg tccatgcggt
     5041 agagctggtc gccggagacg atgaggtagt ggctggggtt ctggggccgg aagtgcatga
     5101 ggttcttgcg cacggcgtcg gccgtgccct cgtaccagcc ggagtggacg agggtctgct
     5161 ccgccgcgag gatctccacg aagccgcgcg agaagctgtc gaagctgtag gtcctggcga
     5221 ggtggaggtg gagggacgcg gagttgaact gggtgaggat atatatctgg cggaagcccg
     5281 agttgatgca gttggagatc gggatgtcga cgatgcggta tttgccgccg aagggcacgg
     5341 ccggcttggc cctgtccttc gtgagggggt agaggcgggt gcccttgccc ccgccgagca
     5401 cgatggagag tactttcggc atgtctaggc tcctgttccg cgcgccttct gtttacgaag
     5461 ggcgcgccat ggcgtatata atagggcatg atcgacgctt ccgccaggac attcatcgac
     5521 gaaatcctcg ccgaccccga tgtcgccccc aggatagccc acgtggagag cgcccccgca
     5581 cgggacgcgg actgggcccc cctccccgac ggcctcgacc cgcgcctcct cgaggccctg
     5641 aaggccaagg gctacgagcg gctctactcc caccagcggg cctcctacga cgcgatccgg
     5701 gccggcgaga acgtcgtcgt cgtcaccccg acggcgagcg gcaagaccct ctgctacaac
     5761 ctgcccgtcg tgcagaccct cctcgaggag ccggaggcca gggccctcta cctctttccg
     5821 acgaaggccc tctcccagga ccagcaggcc gagctcaacg aggtggccct gggcggctcc
     5881 ctccccgtcg ggatctacac ctacgacggg gacacgccga gctccctccg agtggccgcc
     5941 cgcgactcgg gcaggatcgt catcaccaac cccgacatgc tccactccgg agtccttccg
     6001 aaccacgcca agtggatccg cttcttcgcg ggcctccgct acgtcgtcgt cgacgaggcg
     6061 cacgcctaca ggggcgtctt cgggagccac gtcgcgaacg tcatccggcg cctgaagcga
     6121 gtcgcggcct tctacggcgc ccatccccgc ttcgtcctct gctcggcgac gataggcaac
     6181 cccgaggagc tcgcctccgc cctcgtcgag gagcccgtgc gcgccctttc ggagaacggc
     6241 gcgccccggg gcgagcggcg cgtggtcttc tacaacccgc ccctcctcga cccggtgcag
     6301 ggcatccgga agagcacggc gacggagtcc gagaagctcg ccctcctcct cctccggcgg
     6361 ggggtgaaga cgatcctctt cgcccgctcc aggctcaagg tggagctcat cgcctcctac
     6421 atctcgaagg ccctggagaa cctgtacacc gggaacggcg gcgtccgcgt gagcccctac
     6481 cgctcgggcc tcctcccgag cgagaggcgg gccatagaga aggggctccg cgagggctcg
     6541 atccagggcg tcgtctcgac gaacgccctg gaactcggca tcgacatcgg cggcctggac
     6601 gcggccgtcg tcgcgggctt cccggggagc ttcgcgagct tctggcagca ggcggggagg
     6661 gcggggcgcc ggggctccgt ctccgtcgcc ttcctcgtcg cctcctcctc gcccctcgac
     6721 cagttcatcc tggcccatcc cgagtacttc ttcggcagga gccccgagtc ggcgcggatc
     6781 gacccggaca acccctacat cttcgccgac cacgcgaagt gcgccgcctt cgagcttccc
     6841 ttcaaggagg gcgagtccct gggcaaggcc ggccccgagg cgacggccga ggtcctctcc
     6901 atgctcgagg aggagggcct ggtccggctc acgggagggc gctggtactg gtcggcggcc
     6961 ggctacccga gcgaggcgat ctccctgcgc tcggcgacgg cggacaacgt cgtcatcgtc
     7021 gacaggacgg gcggcggggc ccgcgtcatc ggcgagatgg acaggccgag cgccaaggag
     7081 ctcatcttcg acgacgcggt ctacatccac ctcgggcgcc agtacaaggt gcggaagctc
     7141 gacctcgcga acaagacctg cttcgtggag gagaaggagg tcgactactg gaccgactcg
     7201 atcgtgaaga ccgacctcga ggtcctcacg gaggacgagg agggcctcct cgccgctcag
     7261 ggcggccggg gcccgctcgc ctcctgggtc ctgggcgacg tcctcgtccg gaccgaggcc
     7321 gagaaataca agaagctccg cttccacacc cacgagaacg tcggctacgg ggagatcagc
     7381 ctcccgccgg aggagatgca gacgaggagc ctcgtcctcc tcctgcctcc ggggagcgcc
     7441 gggggcgacc tcgtcgcggc ctgccccgag ggagggaagg cggccctcct cgcgggcgcc
     7501 gcccgcctca tccacgacct ggcccccgtc ttcctcatgt gcgacggccg cgacctgggc
     7561 ctcgcggagc gggtccgcga tccccacttc ggcgtcccgg cgatctactt ctacgaccgc
     7621 tatcccggcg gcacgggcct ggccgagggc ctcgcggagc tcctcgggcc cgcgatccgc
     7681 gccgccaggg agcgcctggc ctcctgcggc tgcgaggagg gctgtccctc ctgcgtcggg
     7741 gtggacttcg gcgaggccgc cggcccccag gcggcgcggg gcgggcgggc cgtcaaggcc
     7801 tcggtcctcg agctcctttc ggccctctcc gaagccgcgc ccggcgatgg ccaagaacct
     7861 taaaggacgg ctcgcccgcc tccgcgagct cggcctcgtc aaggctgcgg agctcgaggc
     7921 cccgggcgct ggtgagaagg gcgccttctc gggcggcgcg gccgaaggcg aggccgcggc
     7981 ccagggtggc gcaccggtgg gcgagggcgg ctcgacctcg ccttcggcta gacggcggcc
     8041 cggggcgggg gcgcgcggag cgcgggccgg gggcggcgaa aggccgggag tgccttcctt
     8101 cctggccggc tggacccaag ccgccgagct cgtatgggag agggagctcc gcttcccctc
     8161 ccatcttccg cccctcatcg accccaggcc cttcgcggcg atgtcccgca aggccgcccg
     8221 cggctcgctc gacgcggcga agcccggagt ccggtcggcg gcgaagcccg gcgcccgggc
     8281 ggcggacggg gcggctccgc tgggcgcggg agggaggccg gaagccgcta cgcggggcgc
     8341 cggcttggcg ggcggggccg acggcctcct cccctcggag agcctccgct tcttcgacct
     8401 ggagacgacg ggactctcgg gcggctcagg cacggtggcc ttcctcgccg ccgtgggccg
     8461 gatcgaggcg ggggagttcg cggtgcgcca gctcttcctc gcggactatc ctggcgagag
     8521 ggcctggctc gaggcgctcc tgtccctcct ttccgagggc tccgtcgtga cgacctacaa
     8581 cggacgggcc ttcgacctgc ccctcctccg gacccgcctc gtgatgaacg ggatgcggcc
     8641 gccgtccttc gcggagatcg acgcgctctt cgtcgcgcgc aggctctggc ggcgggtcca
     8701 cgggggagcc tccttgagcc tcctcgagcg gctggtcctg gacctggagc gggagctcga
     8761 cgtgccgggc tcggcgattc cggacatctg gttctcctac ctcagggagg gggaccatcc
     8821 cctcatggcc gcggcgatgg cgcacaacgc ggaagacgtg tccggcctgg agcggctcgc
     8881 cgcccgggcc gcccggatct tcgaggagcc ccgcgcctgg gccgcctcgg gcgccgtgga
     8941 tcgcgccggg ctggggaggg gcctcctcgc gctggggcgc gccgacgagg gcgaggagct
     9001 cctcgaggcc gcgctcggcg agggggacgc ggccgccggc ctcctcctct cgcggcgcta
     9061 caggcgcgag gggcgcctgg aggacaggcg ccgcgtcgtc gggatgcttc ccgagggcct
     9121 tgagagctgc gtggagaggg ccaagttcca cgagcacgcg ctaaaggact tccgggccgc
     9181 cctcgcgtgg acggaccgcg ccatctcctg ggcgcgggag ggcggcggga ccgaagcgtt
     9241 cgaggcgctc gaggccaggc gggccaggct cctgcggaag ctggagaggg gcgggtaggt
     9301 ggaggcggcg cgcgcgtcgg gacgcgccgc gcggtggcct ggagggggcg cggggaaggc
     9361 cgttgcggcg cggccggacg ggcgcggccc ggccgacagc ggccggcgac tctacttgga
     9421 caggagcgtg tcgccgagct ggccgcaggc gcccatgacg ccgcggccgc ggcgcatgcg
     9481 ccgggtcgag gtcaggcccg cctcgtccag gatggcctgg aaggtctcga cctcgtcgcg
     9541 agtcggctcg acgaagggca ggccggggac gcggttccag gggatgaggt tcacctgggc
     9601 cttgaggggc cggatccagc ggatgagggc gcgggcgtcc tcgggacggg agttccggcc
     9661 gcccatcagg gccgcctcca gcgtcacgcg ctcccccgtc gcctcctggt acttgaggag
     9721 ggcggccttg agctccgcga ggccccactt ccggttgacc ggcatgagct cgctccgcaa
     9781 gtcgtcgtcg gcggcggtca gggatatggc gaggcgcagc ttggggccgt gctccgcgag
     9841 gtccaggatg cccggcacga ttccgcaggt ggagatcgtg atcctgcgcg gggagaggcc
     9901 tatgcccttc gggtccgaga ggatggagac ggccttccgc acggcggcga ggttgaggag
     9961 gggctcgccc atgcccatga agacgatgtt cgagatgtcg ccgcgccttc tcttgagctc
    10021 gtggtactgc tccaggatct cgtcgggccc caggtcccgg aggtagccca gggtgccggt
    10081 cttgcagaag gcgcaggcca tggggcagcc gacctggctc gagaggcagg cggtcttgcg
    10141 cccctcgatg tcctcgagga ggacgcactc gacggcggcg ccgtcgcgga ggcggagctg
    10201 gagcttgagg gagccgtcgg ggtcctcgag cgcggccgcg gcctcggagg agtagatccg
    10261 cgggaagagg gccgagagcc gctcgcgctc gggcttcgga aggtcgctca tctcctcgaa
    10321 ggaggaggcc ccgcgggcca gccagcggta gacctggcgg ccgcggaagg cggccttgaa
    10381 gccgcaggcc gcggccacct cggccgggcc catgcccgtc agggagagct ccacctaggc
    10441 ccgctgcagc ttggcgacgg cgtcctggac gtagacgttg cggatgccca ggcgctggaa
    10501 gtcgaccagg gcctccaggg cgcgctgctt gtcgcccaag cctacgtagc actgggagag
    10561 ctccaggtag aggcggtagt tcttggggtc gttctgcacg agcttgcgga ggctcgtgac
    10621 ggcgtcctgg tacttgccct gcatccgcgc gatcacggcc agacccagga ccgcgtagac
    10681 gtcgaactcg atgttgaggg cgcgctcgta gaactccgcg gccttgtcgt actcgcccat
    10741 gctgcggtag gcgtcgccgc cgcgcgtgag gatgaccttg ttgcgcgggt ccttggcgag
    10801 gatctgcttc cagtagtcga gggagcggtg ctgctggccg gagccgcggt agcagtcggc
    10861 gaggccgaag agggcgtaga agttgtcggg ctccttgccc agggcccgct cgaagtaggg
    10921 gacgccgagg tcgaactgct tgagcttgcg gtagcagttc ccgatggagg tgaggacgcg
    10981 gatgtccacg ccctcgccct ggagctcgac catgcgctgc cagtagccca gcgcgtcctt
    11041 gtactccttg aagtcgtagt gcaggtgccc gaggccgatg agggcgtagg ggttgtcctg
    11101 ctccatctcc aggaccctga ggtagatggc cttcgacttc ctgaagtcgc gcaccttgcg
    11161 gtaggcgtcg gcgacgcggg tgaggacggt gatgttcttg ttgtcgtgga ggaggtactg
    11221 ctcccagatc tcgatggc
//
LOCUS       DAOOSJ010000058        11169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3852_length_11169_cov_4.900756, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000058 DAOOSJ010000000
VERSION     DAOOSJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3852_length_11169_cov_4.900756"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /gene="iadA"
                     /locus_tag="PLG14_04405"
     CDS             <1..1178
                     /gene="iadA"
                     /locus_tag="PLG14_04405"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOX48407.1"
                     /translation="MFLLVKNADVFAPERLGRRDILAAGGKILAMAERIDPSGLPGEV
                     EVLDAEGLFLAPGLIDAHVHAIGGGGEGGYATRTPELRASDCLRAGVTTVVGTLGTDG
                     VARSMEALVAKAYALREDGVSAFCYTGSYRVPPRTVTGDAMKDIMMVEPVIGVGEVAL
                     SDHRSSLPGLAELARLASEARVGGMLSGKAGVVNFHIGDAPAALSPLEELVASGELPR
                     SQFLPTHCNRSRAVFESALAWARAGGCADFTTSTVQRFLDDGELGASKALRLFREAGI
                     PLERATFTSDGQGSLPRFDAEGNFAGLGLGSSASLWEALREAILAEGLPPEEALKPAT
                     SSPAAILKLRGKGRVALGADADLILVDPREYRLAATISGGKVVAREGKALAAERFES"
     gene            1229..3409
                     /locus_tag="PLG14_04410"
     CDS             1229..3409
                     /locus_tag="PLG14_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX48408.1"
                     /translation="MKRVCLVAGSANTRLELERQLAEYLEGGAELLSYSIDAGIDRPI
                     EGDLVVASSELVGEELSSSGLLAPGTSLLVGRRTVDCAHLDLVVALPPGTKALFVNDR
                     PETAEACIAALAELGLDGVDYLPYYPGAPSPPPDIGIAVTAGEAALVPPGIAEVIDIG
                     VRILDFGTIAEILVRLGLLERGVGSFSRRYLAAVVSVARRLARSTEETRRLNAHLSSV
                     IDSLSHGILVYDASGRVAVCSENLRSLLGLRPGEGPGRSLPSIIRNAGLLSFLEGPGA
                     EESGVFRLAEGEVLVSRFRAEGGSTIAAFRDDRGADAEGRRLAREYRKRGHVAKYTMD
                     DIVGSGEPILRAKRIALRLAGTDLSILIHGESGTGKELFASAIHAASGRASGPFLAVD
                     LGSLSDDLIESELFGYEEGAFTGAKKGGKAGLFELADGGTLFLDEIGNISPKVQRRLL
                     RVLQEKEVLRVGGSEIKRVDVRVIAATNEDLLERAARGEFREDLYFRLKMGWIRIPPL
                     RERIEDIPILVARLLSLEGARDIPVAPEVLEALAARDWPGNVRELRNTLTYMLAVREG
                     PGLGPGDFPEASYFAQAACLDSGAAGKGRGLPESLAKRFAPASFRSGDASGAFAAAAA
                     AAGFVGAALFAAGLDAGPAAAAGLEPEDRFVLLAVAELEAGGRHAGREAVAALCAGRG
                     LKLGPGAVRARFDRLAARGYIVSSRGRGGTSLTEEGRLLLEAQA"
     gene            3631..4887
                     /locus_tag="PLG14_04415"
     CDS             3631..4887
                     /locus_tag="PLG14_04415"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOX48409.1"
                     /translation="MSNGDLVIVGGAEMLERKSILSRFVELSGGPEGRFLVLASATGS
                     PLEGFERQRRWLGEAGVAPERVELLRVSGKVDGWERGAWDGAELDRLEHADGLWLLGG
                     DQNLTIALLQDEAGGDSPLLAAIRRRAARSHAEGGLVVGGTSAGAAVLSDPMLCAGTS
                     FGALALPRAKGPGEAEMSPALLVGRGFGLFPEGVVDQHFDARARLARLAEAALVEDGA
                     RRPAFGIAEATALVYEGGPRRISVTGAGTVCVLDPRGAKRSLVPTKAGPRLRIEDLLF
                     HCLTEGDSYFPGEERVDFGLKEELPPSGAAFASERPEASGVLSPYSRLADFAARMLLD
                     NAPGLLFLDPRSGRRYARSLLVEETGLPEGGSACLGWEIRLGRHLPGREGPGRKEGRL
                     YYDGRYSFENVYVDILPLEIDINRPE"
     gene            4993..6882
                     /locus_tag="PLG14_04420"
     CDS             4993..6882
                     /locus_tag="PLG14_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX48410.1"
                     /translation="MRRSIARAAIALALIAASAAGAAPAVDRYDATYVAEGSSLRYFY
                     TPDTTDQRVAANTQDGLVENDKYGRFVPSIAESWSSNPDYTEWTFKLRKGLPWVDSRG
                     QKTKYEITADDYVEGFRYVADPKNGIKNLSKDIRKLIAGLNNYYLDLVDIDAGKKTDI
                     TRAKALEAFDASVGVKAPDKYTVVFKLTKPTPFFLSFLVMELFLPVEKEFLASVGPDF
                     GVAKEKILYSGAYYIADWQRDKQIVLKANPFYWDAKAIKVKTVSLQKVVSADVQIQMF
                     QRGELSSATLAADQVKALANTKWADYIYPAEPSSVTYWFVQNFTSPNPEFKAFVNNLN
                     FRKALYYGIDRAKLNELDDPYRPANILRNTVVPEQVVVDEKGRDYTEYPGVKEIRAAG
                     NYYDKQKAREYFAKAVAELTDGKGTIKGVAAGKVDMKPIAEFQVDGKLPLQLLYVSLP
                     DAVETKRALLLKAMLEDVFGKENIEVKLGQAIDDIFAEVVEPRRFDMLNDNFRFGFGD
                     PSAQLGRLVTNGAINDGQYSDPEYDKLVEEAAAKNVLSERYKTFARAEALFLDRCYVL
                     PWKTGGAAYTISKMVPYTYPRGGFGITRFKYKGMVVEKDPVTAKRYEEIKTQFFKELQ
                     AITSK"
     gene            7027..7983
                     /locus_tag="PLG14_04425"
     CDS             7027..7983
                     /locus_tag="PLG14_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48411.1"
                     /translation="MGTYILKRLGQSLVTVVIVVISVFMLLRLMPTTGYFTREDYVNL
                     DATARDAHLRNLGVLDDPVTQLGRFAKGLATGDLGRSISIYPRTPVAAIIAEKAPYSA
                     YFGLVSIAISMALGLGLGVLMARHKDGIIDGAGTVYVTIVRAIPSLIWLFLIQQWVSG
                     LMGWPMVFYEDRPVSWILPTVSFSLSGIAWYAIWLRRFMVDEENRDYVKFATAKGLPK
                     RRVARGHVLRNALVPLVQYFPQQILLTIAGSLLIESIYSIPGMGGLLVNAIRQQDNPI
                     VQALVLLYAVLGVLGVFLGDLLMVFVDPRIKLEGPSAKGAEA"
     gene            7980..8912
                     /locus_tag="PLG14_04430"
     CDS             7980..8912
                     /locus_tag="PLG14_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48412.1"
                     /translation="MRRDLPDLGPELFRPAPFDESKAELTGYARYSYWRSTFRTFLKS
                     PVSVFLLALILVLVTASFVYPVLSPVDPNQVELDTSAWNARPGNGHVFGTDGLGRDIW
                     ARTWHGTRTSMLLGLVIALFDVGIGMAAGALWGYVRKIDRIMTELYNVLNNIPQTVFL
                     ILLTYIMRPGFWTIVVALCSTGWIGIARFVRNKVITIRDAEYNVASRCLGTPLRRMIT
                     RNVLPYLVSILIMEAALTIPYSIGSEVFLGFIGLGLPIETVSLGNLVNLGKNNFMLYP
                     YQLTWPTLVLAAITVSFYIVGNRFSDASDPRNHV"
     gene            8909..10084
                     /locus_tag="PLG14_04435"
     CDS             8909..10084
                     /locus_tag="PLG14_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48413.1"
                     /translation="MSGRDDKRSQAATGPAKTAAAAPGAQTASGAAAAPILEAKDLSI
                     SFSLRGRRLRAVRGASLSLRAGETLAVVGESGSGKSVLSKSFLGMLDKNGRVDSGSIL
                     FGGEDLAAYTKERQWLQVRGKRIAMIFQDPMTALNPLKTVGEQIREAAALHRGLDRKT
                     SRLAVLETLERVGIAEPEARYRQYPHEFSGGMRQRAVIAAAVACRPEVLICDEPTTAL
                     DVTVQAQILDLLKSLQKDFGMSILFITHDLGVVAKVADRVAVMYAGQVIEEGLSREVF
                     YDPRCPYTWALLASLPQLGVKGEELYSIKGTPPSLFKEIKGDAFAPRNPKALLVDYEA
                     EPPVFEVSPTHWARTWLLDPRAPRVEAPDTIARLRGRLEARAAADAGARGPGEGGKA"
     gene            10081..11022
                     /locus_tag="PLG14_04440"
     CDS             10081..11022
                     /locus_tag="PLG14_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48414.1"
                     /translation="MSGEAILSVRGLGVDYPVGRKLFPAVRGVSFDIRKGETFSVVGE
                     SGSGKTTIGRAILRVAPVSRGEILFKGERISGRIPAERERALRQGIQMIFQDPMASLN
                     ERAKIDYIVSEGLYNFRLFKDEAERRAKVEKALREVGLLPEFASRFPHEFSGGQRQRI
                     GIARALVMEPELLVADEPISALDVSIRAQVINLMNRLKRDLGLTYLFIAHDLSVVRFI
                     SDRIAVIYRGRLVELAEAEALFARPLHPYTRALLSAVPLPDPEIESRRRFVVYDPSMH
                     DYSKEEPEWIEAEGGHFVLGTPRELEAWSAGPGGAGA"
BASE COUNT         1688 a         4203 c         3631 g         1647 t
ORIGIN      
        1 acatgttcct tctcgtcaaa aacgccgatg ttttcgcccc ggagcgcctg gggcgtcgcg
       61 atatcctggc cgcgggcggc aagatcctgg ccatggccga gcggatcgac ccttccggac
      121 tacccggaga ggtcgaggtc ctggacgccg agggcctctt cctggccccc ggcctcatcg
      181 acgcccacgt ccacgccata ggcggcggcg gggagggcgg ctacgcgacc cggacccccg
      241 agcttcgggc ctcggactgc ctcagggcgg gagtcacgac ggtcgtgggc accctgggca
      301 ccgacggcgt cgctcggagc atggaggccc tggtcgcgaa ggcctacgcc ctgcgcgagg
      361 acggggtctc ggccttctgc tacacgggga gctaccgcgt ccccccgcgg accgtcacgg
      421 gcgacgcgat gaaggacatc atgatggtcg agccggtcat cggcgtcggc gaggtggccc
      481 tctccgacca ccgctcctcc ctgcccggcc tggccgagct agcccgcctc gcctcggagg
      541 cgagggtcgg cggcatgctc tccggcaagg cgggcgtcgt caacttccac atcggcgacg
      601 ccccggcggc cctctccccc ctggaggagc tcgtcgcctc tggcgagctc ccccgctccc
      661 agttcctccc cacccactgc aaccggagcc gggccgtctt cgagtcggcc ctggcctggg
      721 ccagggcggg cggctgcgcc gacttcacga cgagcacggt ccagcgcttc ctggacgacg
      781 gggagctggg ggcctccaag gccctgcgcc tcttccgcga ggcgggcatt cccctggagc
      841 gggcgacctt cacctccgac ggccagggct ccctgccccg cttcgacgcg gaggggaact
      901 tcgccgggct gggcctggga agctcggcgt cgttgtggga agcgctcagg gaggctatac
      961 tcgccgaggg cctgcccccg gaggaggccc tgaagcccgc gacgagctcc ccggcggcca
     1021 tcctcaagct ccgcggcaag ggaagggtgg ccctcggggc cgacgccgac ctcatcctcg
     1081 tcgacccgcg cgagtacagg ctcgcggcca cgatctcggg cggcaaagtc gtcgcgcggg
     1141 aaggcaaggc cctggcggcc gagcgcttcg agtcctgagt gcggccggcc cgaggcgcgg
     1201 gccgaaggcc gcccccaagg aggcgtagat gaaacgcgtc tgtctcgtcg cgggaagcgc
     1261 gaacacgcgc ctcgagctcg agcgccagct cgcggagtac ctcgaaggcg gggccgagct
     1321 cctctcctat tcgatagacg ccggcataga ccggcccatc gaaggcgacc tcgtcgtcgc
     1381 ctcgagcgag ctcgtcggcg aggagctcag ctcctccggc ctcctcgcgc ccgggacgag
     1441 cctccttgtc gggcggcgca ccgtcgactg cgcccacctg gacctcgtcg tcgccctccc
     1501 gccgggcacc aaggccctct tcgtgaacga ccgtccggag acggcggagg cctgcatcgc
     1561 ggccctggct gagctcggcc tggacggcgt cgactacctc ccctactacc ccggcgcgcc
     1621 cagcccgcct cccgacatcg gcatagccgt caccgccggc gaggcggccc tcgttccgcc
     1681 gggcatagcc gaggtgatcg acatcggcgt ccgcatcctg gacttcggca ccatcgcgga
     1741 gatcctggtc aggctcggcc tcctggagcg cggcgtcggg agcttctcgc ggcgctacct
     1801 cgccgccgtc gtctccgtgg cgcggcgcct ggcccgctcc acggaggaga cgcgcaggct
     1861 caacgcccac ctctcgagcg tcatcgactc cctctcccac ggcatcctcg tctacgacgc
     1921 ctcgggcagg gtcgccgtct gcagcgagaa cctccgcagc ctcctcggcc tccgccccgg
     1981 cgaggggccg ggacggagcc tcccctcgat catccggaac gccggcctcc tctccttcct
     2041 ggaggggccg ggcgcggagg agtcgggcgt cttccgcctc gcggagggcg aggtcctcgt
     2101 gagccgcttc cgcgcggaag gaggctccac gatagcagcc ttccgcgacg accggggcgc
     2161 cgacgccgag ggccggcgcc tggcccggga gtaccgcaag cgcggccacg tcgccaagta
     2221 cacgatggac gacatcgtcg gctcgggcga gcccatcctc cgcgccaagc gcatcgccct
     2281 gcgcctggcg gggacggacc tctccatcct catccacggc gagtcgggca cggggaagga
     2341 gctcttcgcg agcgccatcc acgccgcctc cggccgcgcc tccgggccct tcctggccgt
     2401 ggacctgggc tccctctccg acgacctcat cgagagcgag ctcttcggct acgaggaggg
     2461 cgccttcacc ggggccaaga agggcggcaa ggcgggcctc ttcgagctcg ccgacggggg
     2521 caccctcttc ctggacgaga tcggcaacat ctcgcccaag gtccagcgcc ggctactccg
     2581 ggtcctccag gagaaggagg tcctgcgggt cggcggctcg gagatcaagc gcgtcgacgt
     2641 ccgcgtgatc gccgccacga acgaggacct cctggagcgg gcggcccgcg gcgagttccg
     2701 cgaggacctc tacttccggc tgaagatggg ctggatccgc atcccgccat tgcgggagcg
     2761 gatcgaggac atccccatcc tcgtcgcgcg cctcctctcc ctggaggggg cccgggacat
     2821 ccccgtcgcg cctgaggtcc tggaggccct ggccgcgagg gactggccgg gcaacgtccg
     2881 cgagctcagg aacaccctca cctacatgct cgccgtgcgg gaggggcccg gcctgggtcc
     2941 gggcgacttc ccggaggcca gctacttcgc ccaggcggcc tgcctcgact cgggcgccgc
     3001 cggcaagggc cggggcctgc ccgagagcct cgccaagcgc ttcgccccgg cctccttccg
     3061 ctcgggcgac gcatcgggcg ccttcgcggc ggcagcggcg gcagcgggct tcgtcggcgc
     3121 ggccctcttc gcggccggcc tggacgcggg cccggccgcc gccgccggcc tcgagcccga
     3181 ggaccgcttc gtcctcctcg ccgtcgccga gctcgaggcc gggggccgcc acgcggggcg
     3241 ggaggcggtc gccgccctct gcgccggccg cggcctcaag ctgggcccgg gggcggtgcg
     3301 cgcccgcttc gaccggctcg cggccagggg ctacatcgtc tcctcccgcg gccggggcgg
     3361 cacgagcctc accgaggagg gccgcctcct cctcgaggcc caggcttgag gcgcaaagcc
     3421 gcagcggcgg aaccttgtgc ggcgccgggg gcgggccgct gcgccttgcc ggacttcgga
     3481 cgccgtggtg ccgcggcgcc gtggtgccgc ggcgccttgc cgtccgccga ggcgtcgaag
     3541 gagcgggcgg cgcaggctgg cgcaccaact ggctattatt ggttatttgt atcctattaa
     3601 ccacctgaaa ccaattacgg agcgccagtc atgagcaacg gagaccttgt catcgtcggc
     3661 ggagcggaaa tgctggagag gaagtccatc ctctcgcgct tcgtcgagct ttcaggcggt
     3721 ccggagggac gcttcctcgt cctggcgagc gcgaccggaa gcccccttga gggcttcgag
     3781 cggcagcgcc gctggctcgg cgaggcaggc gtggcgcccg agcgggtcga gctcctccgc
     3841 gtctcgggca aggtagacgg ctgggagcgg ggcgcctggg acggggccga gctcgaccgc
     3901 ctggagcacg ccgacgggct ctggctcctg ggcggggacc agaacctcac gatagccctc
     3961 ctacaggacg aagcgggcgg ggactccccc ctcctcgcgg cgatccgccg ccgcgccgca
     4021 agaagccacg ccgagggcgg cctggtcgtc ggcggcacga gcgcgggcgc ggccgtcctc
     4081 tccgacccca tgctctgcgc gggcacgagc ttcggggccc tggccctgcc gcgggcgaaa
     4141 ggcccgggcg aggcggagat gagccccgcc ctcctcgtgg ggcgcggctt cggcctcttc
     4201 cccgagggcg tcgtcgacca gcacttcgac gcgagggccc gcctggcccg cctcgccgag
     4261 gccgccctcg tggaggacgg ggcgaggagg ccggccttcg ggatagcgga ggcgacggcc
     4321 ctggtctacg agggcggccc gcgcaggata agcgtcacgg gcgcggggac ggtctgcgtc
     4381 ctcgacccgc ggggcgcgaa gcggagcctt gtaccgacga aggccggccc ccgcctgagg
     4441 atagaagacc tccttttcca ctgcctgacc gagggcgaca gctactttcc cggggaagag
     4501 agggtcgact tcggcttgaa ggaggagctc ccgccctcag gcgcggcctt cgcctctgag
     4561 cggcccgaag cctcgggagt cctctcgccc tactcccgcc tcgccgactt cgccgcccgg
     4621 atgctcctcg acaacgcccc tggcctcctc ttcctggacc cgcgctcggg acggcgctac
     4681 gcgaggagcc tccttgtcga ggagacaggc ctccccgaag gcggctcggc ctgcctgggc
     4741 tgggaaatcc gcctgggcag gcacctgccg ggccgggagg gcccggggcg caaggagggc
     4801 cgcctctact acgacggccg ctactccttc gagaacgtct acgtggacat ccttcccctc
     4861 gagatcgaca tcaatcgccc ggaatgagct tgaaatggta cggttcttgc taaaagtacc
     4921 agtggagaaa tccgtactgt cttaaaggag gcggtcgggc tccgtgaaga ctacgcattt
     4981 ctggaggcca gcatgaggag aagcattgca cgcgcggcga tcgccctcgc gttgatcgcg
     5041 gcgtcggcgg cgggagcggc tcccgccgtg gaccgctacg acgcgaccta cgtcgcggag
     5101 ggatcctccc tccgctattt ctacaccccc gacacgacgg accagcgcgt cgcggcgaac
     5161 acccaggacg ggctcgtcga gaacgacaag tacggccgct tcgtcccctc catcgccgag
     5221 tcctggtcga gcaaccccga ctacacggaa tggaccttca agctcaggaa gggccttccc
     5281 tgggtcgact ccaggggcca gaagacgaag tacgagatca ccgccgacga ctacgtcgag
     5341 ggcttccgct acgtcgccga ccccaagaac gggatcaaga acctctccaa ggacatccgg
     5401 aagctcatcg ccggcctcaa caactactac ctggacctcg tcgacatcga cgcgggcaag
     5461 aagaccgaca tcacccgcgc caaggccctc gaggccttcg acgcgtccgt gggcgtgaag
     5521 gccccggaca agtacacggt cgtcttcaag ctcacgaagc cgacgccctt cttcctctcc
     5581 ttcctcgtga tggagctctt cctccccgtg gagaaggagt tcctcgcctc cgtcggcccc
     5641 gacttcggcg tcgccaagga gaagatcctc tactcgggcg cctactacat cgccgactgg
     5701 cagcgggaca agcagatcgt cctcaaggcc aaccccttct actgggacgc gaaggcgatc
     5761 aaggtgaaga ccgtctccct gcagaaggtc gtgagcgccg acgtgcagat ccagatgttc
     5821 cagcgcggcg agctctcgag cgcgaccctc gcggccgacc aggtgaaggc cctcgcgaac
     5881 acgaagtggg cggactacat ctaccccgcg gagccctcct ccgtcaccta ctggttcgtc
     5941 cagaacttca cctccccgaa ccccgagttc aaggccttcg tcaacaacct gaacttccgc
     6001 aaggccctct actacggaat cgaccgcgcc aagctcaacg agctcgacga cccctaccgg
     6061 cccgcgaaca tcctgcgcaa cacggtcgtg cccgagcagg tcgtcgtgga cgagaagggc
     6121 agggactaca cggagtaccc cggcgtcaag gagatccgcg ccgccggcaa ctactacgac
     6181 aagcagaagg cgagggagta cttcgccaag gccgtcgccg agctcaccga cggcaagggg
     6241 accatcaagg gcgtcgcggc cggcaaggtg gacatgaagc cgatcgccga gttccaggtc
     6301 gacggcaagc tccccctcca gctcctctac gtctccctcc ccgacgccgt ggagaccaag
     6361 cgcgccctcc tcctcaaggc catgctcgag gacgtcttcg gcaaggagaa catcgaggtc
     6421 aagctcggcc aggcgatcga cgacatcttc gccgaggtcg tggagccccg ccgcttcgac
     6481 atgctcaacg acaacttccg cttcggcttc ggcgacccga gcgcccagct cggccgcctc
     6541 gtgacgaacg gcgccatcaa cgacggccag tactccgacc ccgagtacga caagctcgtg
     6601 gaggaggccg ccgccaagaa cgtcctctcc gagcgctaca agaccttcgc ccgcgccgag
     6661 gccctcttcc tggaccgctg ctacgtcctc ccctggaaga cgggcggcgc cgcctacacg
     6721 atcagcaaga tggtgcccta cacctacccc cgcggcggct tcggcatcac ccgcttcaag
     6781 tacaagggca tggtcgtcga gaaggatccc gtcaccgcca agcgctacga ggagatcaag
     6841 acccagttct tcaaggagct ccaggcgatc acctccaagt gacgcctccc gtccggggcc
     6901 ttcgggcccc gggcggccgg acccgcgccg aaggcggccc tcccgggccc tgcccggcgc
     6961 gggcccttcc cgggcggccc gcctccgcgc gcggccgcat acccacttcc ggcacagagg
     7021 attgcgatgg gcacctatat ccttaagcgg ctcggccagt cgctcgtcac cgtggtcatc
     7081 gtggtgatct ccgtcttcat gctcctcagg ctcatgccca cgacaggcta cttcacccgc
     7141 gaggactacg tgaacctcga cgcgacggcc cgcgacgccc acctgcggaa cctgggcgtc
     7201 ctggacgatc ccgtgaccca gctcggccgc ttcgcgaagg gcctcgcgac gggcgacctg
     7261 gggagatcca tctccatcta cccgcggacg cccgtggccg cgatcatcgc ggagaaggcg
     7321 ccctactccg cctacttcgg cctcgtctcc atcgccatca gcatggccct gggcctgggc
     7381 ctgggcgtgc tcatggcgcg gcataaggac gggatcatcg acggcgcggg cacggtctac
     7441 gtgacgatcg tccgcgcgat cccgagcctc atctggctct tcctgatcca gcagtgggtc
     7501 tccggcctca tgggctggcc catggtcttc tacgaggaca ggcccgtctc ctggatcctg
     7561 ccgaccgtct ccttctccct ctcgggcata gcctggtacg cgatctggct caggcgcttc
     7621 atggtcgacg aggagaaccg cgactacgtg aagttcgcga ccgcgaaggg cctccccaag
     7681 cgccgcgtcg cgaggggcca tgtcctcagg aacgccctcg tccccctggt gcaatacttc
     7741 ccccagcaga tcctgctcac gatcgcgggc tccctcctca tcgagagcat ctactccata
     7801 cccggcatgg gaggcctcct cgtgaacgcg atacggcagc aggacaaccc catcgtccag
     7861 gccctcgtcc tcctctacgc cgtcctaggc gtcctgggcg tcttcctcgg ggacctcctc
     7921 atggtcttcg tggacccgcg catcaagctc gagggcccct cggccaaggg ggcggaggca
     7981 tgaggcgcga ccttcccgat ctcgggccgg agctcttccg tcccgcgccc ttcgacgagt
     8041 cgaaggccga gctgacgggc tacgcccgct actcctactg gcgctcgacc ttccgcacct
     8101 tcctgaagag cccggtctcc gtcttcctcc tcgccctgat cctcgtcctc gtgacggcga
     8161 gcttcgtcta tcccgtcctc tcccccgtcg accccaacca ggtcgagctc gacacctccg
     8221 cctggaacgc gaggcccgga aacggccacg tcttcggcac ggacggcctg gggcgcgaca
     8281 tctgggcgcg cacctggcac gggacgcgga cctcgatgct cctgggcctc gtgatcgccc
     8341 tcttcgacgt cggcatcggc atggcggccg gggcgctgtg gggctacgtg cggaagatcg
     8401 accggatcat gacggagctc tacaacgtgc tcaacaacat cccgcagacg gtcttcctca
     8461 tcctcctcac ctacatcatg cggcccggct tctggaccat cgtcgtggcc ctctgctcca
     8521 cgggctggat cggcatcgcc cgcttcgtgc gcaacaaggt gatcacgatc cgcgacgcgg
     8581 aatacaacgt cgcgtcccgc tgcctgggca cccccctgcg gcggatgatc acgcgtaacg
     8641 tcctgcccta cctcgtgagc atcctcatca tggaggccgc cctcaccatc ccctattcga
     8701 tcggctcgga ggtcttcctc ggcttcatag gcctggggct gcccatcgag accgtgtcgc
     8761 tcgggaatct cgtcaacctc gggaagaaca acttcatgct ctacccctac cagctcacct
     8821 ggccgaccct cgtcctcgcg gcgatcacgg tctccttcta catcgtcggg aaccgcttct
     8881 ccgacgcctc cgacccgagg aaccacgtat gagcggccgc gacgacaagc gctcccaggc
     8941 cgccaccggc ccggccaaga ccgcggccgc ggcgcccggc gcccagacgg cctcaggcgc
     9001 cgcggcggca cccatcctcg aggcgaagga cctctccatc agcttctccc tccgcggccg
     9061 gcgcctcagg gccgtgcgcg gcgcctccct ctccctccgc gcgggcgaga ccctcgccgt
     9121 cgtgggcgag tcgggctccg ggaagagcgt cctctccaag tccttcctcg gcatgctcga
     9181 caagaacggc cgcgtggact cgggctccat cctcttcggc ggcgaggacc tcgccgcgta
     9241 cacgaaggag aggcagtggc tccaggtccg cggcaagcgc atcgcgatga tcttccagga
     9301 cccgatgacg gccctgaacc ccctcaagac cgtgggcgag cagatccgcg aggccgccgc
     9361 cctccaccgg ggcctcgacc gcaagacctc ccgcctggcc gtcctcgaga ccctggagcg
     9421 ggtcggcatc gccgagcccg aggcccggta ccgccagtac ccccacgagt tctccggcgg
     9481 catgcgccag cgcgccgtca tcgcggcggc tgtcgcctgc aggcccgagg tcctcatctg
     9541 cgacgagccg acgacggccc tggacgtgac ggtgcaggcc cagatcctcg acctcctcaa
     9601 gtccctccag aaggacttcg gcatgtcgat cctcttcatc acccacgacc tgggcgtcgt
     9661 cgcgaaggtc gccgaccgcg tcgctgtcat gtacgcgggc caggtcatcg aggagggcct
     9721 ctcccgcgag gtcttctacg acccgaggtg cccctacacc tgggccctcc tcgcctccct
     9781 gccccagctc ggggtgaagg gcgaggagct ctactccatc aagggcacgc cgccgagcct
     9841 cttcaaggag atcaagggcg acgccttcgc ccccaggaac ccgaaggccc tcctcgtcga
     9901 ctacgaggcc gagccgcccg tcttcgaggt gagcccgacc cactgggccc ggacctggct
     9961 cctggacccg cgcgcgccgc gcgtcgaggc gccggacacg atagcgcggc tccgcgggcg
    10021 cctggaggcg cgcgcggccg ccgacgccgg ggcccgcggg cccggcgagg gaggaaaggc
    10081 atgagcggcg aggcgatact ctcggtccgc ggcctcgggg tcgactaccc cgtgggccgg
    10141 aagctcttcc cggccgtgcg cggcgtgagc ttcgacatca ggaagggcga gaccttctcc
    10201 gtcgtcggcg agtcgggctc cggcaagact acgatcggtc gggccatcct ccgcgtcgcg
    10261 cccgtgagca ggggagagat cctgttcaag ggcgagcgca tctccggaag gatccccgcg
    10321 gagcgcgagc gggccttgag gcaaggcatc cagatgatct tccaggaccc gatggcctcc
    10381 ctcaacgagc gggcgaagat cgactacatc gtctccgagg gcctctacaa cttccgcctc
    10441 ttcaaggacg aggccgagcg ccgcgccaag gtggagaagg ccctccgcga ggtcggcctc
    10501 ctccccgaat tcgcgagccg cttcccccac gagttctcag gcggccagcg ccagcgcatc
    10561 ggcatcgccc gcgccctcgt gatggagccc gagctcctgg tcgccgacga gccgatctcc
    10621 gccctcgacg tctccatccg cgcccaggtc atcaacctca tgaaccgcct gaagcgggac
    10681 ctcgggctca cctatctctt catcgcccac gacctctcgg tggtgcgctt catctccgac
    10741 cgcatcgcgg tgatctaccg gggccgcctg gtcgagctgg ccgaggccga ggccctcttc
    10801 gccaggcccc tccatcccta cacccgcgcc ctcctctcgg ctgtgcccct ccccgacccc
    10861 gagatcgaga gccggcggcg cttcgtcgtc tacgatccct ccatgcacga ctactcgaag
    10921 gaggagccgg agtggatcga ggcggagggc gggcacttcg tcctcgggac gccgcgggag
    10981 ctcgaggcct ggtcggcagg ccctggcggc gccggggcct aggcgccccg cctggcggcg
    11041 cggggcctag tcacgggagg cgcgggaccc gcgggcccag tcgccgcgcg ggcccaatcg
    11101 ccgctcgggc ccagtcgccg ccgggcgctc agagccgcgg cgcggcggaa gcaagcatca
    11161 agggggctg
//
LOCUS       DAOOSJ010000059        11081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3884_length_11081_cov_6.811627, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000059 DAOOSJ010000000
VERSION     DAOOSJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_11081_cov_6.811627"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PLG14_04445"
     CDS             complement(<1..1277)
                     /locus_tag="PLG14_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48415.1"
                     /translation="MTVGQKANLSLLIAVLVFAAFSVAAFSGLFDVVEARFYNPSITA
                     AYEKSLDSMAEASDLYHGLNLERFSAVLGKDAVKRSFLPNLSAEDAFERTNALGKLQE
                     ETPGLAGIRLIDSNGKRIHFSNVPGDILKQSQLETLYRNYGEAGDPPYASLEAPDASA
                     GAVRLDPQAGRYVYSLPFRDSFGVYRGTAVFLVSQGGLLSSFLKAGLVSVGDDVLAAG
                     DRGILLKTPVPGRAALAARAAELWTAGMPEGPVPLAGGEGLDSLVLFSKSTARAGYLG
                     LIVPASAFVFPATMKWLLLGSFFVTAYLLSFLLLNLRQDRMAVLSDRIKRFQITLLEE
                     YLDNKADIDFDRWRRELEARRGEVRGEIKKSVGPFRKGREGEVDELIDKSWDEILAVL
                     GRKSETGILGAGALGVKEVERLIAEALKNGSFVL"
     gene            1535..3400
                     /locus_tag="PLG14_04450"
     CDS             1535..3400
                     /locus_tag="PLG14_04450"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48416.1"
                     /translation="MRLRDFSLKFKVLFMTIFGVALIAFVIAAFWVKDIVEMATLGIL
                     EKSRGVVLTAEAARDAMADRLGAGVITDLAQLAEEGDREKLLKAVPILTAIEVAAKNA
                     EESGYEFRVPKFSPRNPANSPVGVEVEALKKLESGELAEYVVREKGQIRYFRPIRLTE
                     DCMLCHGEPAGEPDPVGGVREGWKVGEIHGAFELVSSLRKAEKDAAKAELNIGGLALG
                     VMVLLGLGLFFVIRIVLKPLGHYVETFKKAATGDLTARADARSRDEVGRVAGFFNDFI
                     ASLESMVDEVRTVAESATAISQDLAASSEETAASLHEIRANTEGMKDKIVRLDAEVGE
                     SARSASDVREFIARLSELIQAQAAAITESSASIEEMSASIANIAKSSEEKLRIANELE
                     VSALDGQSEMEETEQVIKKVADSATVIMEMIQIIQDIASRTNLLAMNAAIEAAHAGEF
                     GKGFAVVADEIRNLAESSAESAGAITDSLGEVAEYIKVSETSTARTGEVFSSIVAQIK
                     DVSASMSEMKNATFELSIGAKQILEALGSLVQTTEEVKNSSREMNTRVAGITEAMDRV
                     SGIASDTRNGMEEVTIGITEIFKAAEAIAEAGTRNSESVRSLKELVGRFTLAGRD"
     gene            3424..5859
                     /locus_tag="PLG14_04455"
     CDS             3424..5859
                     /locus_tag="PLG14_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOX48417.1"
                     /translation="MDALERFTEEAADRLRAEIEEAGGNEVFAACRLDAEGRISEVLV
                     VARGHESAVPALAPYIERGDIVVHNHPSGTLRPSDADVNVAAEAGSKGVGSYIVDNGV
                     RSVYVVAEAARRKAYSLLDEDELAGVLEAGGKLSTLIPSFEPRPSQVRLARDIARAYN
                     EERVLAAEAGTGVGKSFAYLIPSFAWAVKNEERVVVSTATINLQKQLVDKDIPVVQRL
                     FRKKTKAVLVKGRGNYLCRVRLREALDEEGLFAEEGSPLKAIAAWAETSATGDRTDLS
                     FWPEDSLWYRVASESESCLGLRCPDRETCFVLRVRREAADAQVIVANHHILFSDLAAR
                     MSGAGYEATAVLPPFRILVIDEAHAVESSATSFFSEELARFGVNKRLSRILRKRGGRS
                     FGIVERLLALPSFPRSRLDELPAAIARAREAMDALDNKAVLLFDRERQYRLTERSPLL
                     EDSLLRPLGELERRLLACAEILREALDEAPEASAGERAVYEARLMLRRLAEDAALCAK
                     FKDFESNPEKVFWLEKARTGQGETFVRYTASPLDITGTMEEAVFTKFRSVVCASATLS
                     VGETFDFWKSRVGLRHTDVPVDTAVYASPFPFRTNALLAVDVSAPGPESPAYKRYVDE
                     AVVELLRASRGRALVLFTAYEALKSAYEKAKPAMEELGIACMRQGEGERGRLLEAFKA
                     DVSSVLFATDSFWEGVDAPGETLSLVVIAKLPFRVPTDPVQLARSEACEKRGGNAFME
                     ISLPEAVIRLKQGFGRLIRHSEDRGAVVILDSRLATKRYGPLFVASLPECRLVAEPLP
                     KILSEVAKFLDDW"
     gene            5909..7144
                     /gene="manA"
                     /locus_tag="PLG14_04460"
     CDS             5909..7144
                     /gene="manA"
                     /locus_tag="PLG14_04460"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOX48418.1"
                     /translation="MRIYSLENPIQRYDWGSPDGLASALGIPNPEGGPAAELWMGAHP
                     KAPSLALVEGRRVALDALVAAEPEAVLGPASVARFGPALPFLFKALSAGAPLSIQAHP
                     AKRKAERGFERENLAGIPRDAPERNYRDANHKPEMAVALTRFEGLCGFRPVEEIVENI
                     RLLSPDGHSRIAGRLERNPGRVELSVLFYSTISAPEEDKQAALARVRPRVERLLAEGG
                     LGPAREKALRWAKRLFELFPGDIGALAPLLLNLVALEPGEAVFIGAGELHAYLEGTAL
                     EIMANSDNVIRGGLTRKHVDVPELISVLTFSPATPVVFGPERVSESEELYPAFAPDFQ
                     LSRLRPRRGSPLRRSPLGPEILLCGEGAFAIREGGGAVELSRGASAFVRADAEEYEIE
                     GEGTLFAASVPRGGRPEEP"
     gene            7141..7641
                     /locus_tag="PLG14_04465"
     CDS             7141..7641
                     /locus_tag="PLG14_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOX48419.1"
                     /translation="MRIYADGDSLQAELRVLLSRRAAAERSRAAARDEEPRFSLLFVA
                     ARKLPTAGGAELLLVEGGPDAADGKMLELALPGDLAVTRDLPLAERLAEKGVSVLNDR
                     GEAFTAENVRERRSLRDRAAELRALGLAPESPRARSWGQKELKAFADAFDRELTRLLK
                     GGTGPR"
     gene            complement(7727..8506)
                     /locus_tag="PLG14_04470"
     CDS             complement(7727..8506)
                     /locus_tag="PLG14_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOX48420.1"
                     /translation="MARAVPGLVLAEGPWIGALPPELRLPPAAPPRRRLSPGRILRAV
                     GKGLLLAQLGYVLATSLLIAVYRFSDPSVTVLMAYRSWGYGWKLVPPRPVALKKVPSY
                     VRRMLVSVEDYKFYEHHGLDFEAFERAYEINSRIKRPLYGGSTLTMQVARTLFLVPEK
                     SYLRKYLEVIAALELELLLPKDRILELYFGYAEWGKGVFGVEAASRAYYGKSLSRLSR
                     EEAARLVALLSSPIKYKPSTLAKNGILRSRYEFLSKRYLEN"
     gene            8711..10177
                     /locus_tag="PLG14_04475"
     CDS             8711..10177
                     /locus_tag="PLG14_04475"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOX48421.1"
                     /translation="MKRFLLALSCALALTGSLFAQATSHPGLFRYRLDNGLELFVYRD
                     ATLPLARVGMSFRAGSSFQAADSAGSFGLLERLAFENGGQAGGPAGASPRLKAAMARI
                     GAAESGGGTGTDSMGYWLRLPAARTGEALRFWAERFVDAPLDPAALAAAKAGALARLA
                     ELKAEPDAVYEAAVAKRLFGKYPWRRDPAGSEAAIAAATPESLARLRDAYLVPGNAAV
                     FVGGDVDPEETRRAVEALFGGWKRADEASRPALPAHPRPGVPRPTWIVHPDASLPEGL
                     AMVEARYRGPDLQSDPQGSYAADLWTALVADPEGRFKKAVAKEVPKLYGRDSISAYYL
                     SQRDGGALSVSAYFSVDPALSSVDRARQFKERVRGFEMTAMRTEPAYFSAADYEAARR
                     RLSDARALSLETADGFLESLAFWWASASADYFLGYPEALAKTGPREVGAFIDAYVMRN
                     LEVVALRMSPADYEREKKSFAGAGFEVVTAANAFWWQR"
     gene            10165..>11081
                     /locus_tag="PLG14_04480"
     CDS             10165..>11081
                     /locus_tag="PLG14_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48422.1"
                     /translation="MAALSAGRGRRGTARALAGCAAGAALLFASCATGAPPAGEAGLR
                     QAAPAGSASAAAGEARPADRRPRQSPYLLASLASIERERLASGVVLLARRLPGRGVAA
                     AELLLLRDGSTPSGKDGVEALALSLAARGGRLPSGGRADLPALVRAAGGSLELKALSE
                     DALALELAAPLDRLPALLEALAASVAAPSFEEADFVAAMRDLRAARLAAEADPARVAA
                     EGLRTEAYRGSPYARPPFGTAESLAALRLEDAAAYWREAVGAERVALVVAGDLGAAEL
                     AARVGPALAALPRKGGAAGLLPEPGSAPA"
BASE COUNT         1641 a         4039 c         3872 g         1529 t
ORIGIN      
        1 aggacgaagc tcccgttctt gagggcctcg gcgatgaggc gctcgacttc cttgacgccc
       61 agggcgccgg cgccgaggat cccggtctcg gacttgcggc ccaggacggc gaggatctcg
      121 tcccagctct tgtcgatgag ctcgtccacc tcgccctcgc ggcccttgcg gaaggggccg
      181 acgctcttct tgatctcgcc gcggacctcg cccctgcgcg cctcgagctc gcgccgccag
      241 cggtcgaagt cgatgtccgc cttgttgtcc aggtactcct cgaggagggt gatctggaag
      301 cgcttgatgc ggtcggagag gacggccatc ctgtcctgcc tgaggttgag gaggaggaag
      361 gagaggaggt aggccgtgac gaagaaggag ccgaggagga gccacttcat cgtggcgggg
      421 aagacgaagg cggaggcggg cacgatgagg cccaggtagc ccgcgcgggc ggtggacttg
      481 gagaagagga cgagggagtc caggccctcg cctccggcca gggggacagg cccctccggc
      541 atgcccgcgg tccagagctc ggcggcgcgg gcggcgaggg ccgcccgccc ggggacgggc
      601 gtcttgagga ggatgccccg gtcgccggcc gcgaggacgt cgtcgccgac ggacacgagg
      661 ccggccttga ggaaggagga gaggaggccg ccttgcgaca cgaggaagac ggccgtgccg
      721 cggtagacgc cgaagctgtc gcggaagggc agggaataga cgtagcggcc ggcctggggg
      781 tcgagcctga ccgccccggc cgaggcgtcg ggcgcctcca gggaggcgta gggcggatcc
      841 ccggcctcgc cgtagttgcg gtagagggtc tcgagctggc tctgcttcag tatgtcgcca
      901 gggacgttcg agaagtggat gcgcttgccg ttggagtcga tgaggcggat gcccgccagg
      961 ccgggcgtct cctcctggag cttgccgagg gcgttcgtgc gctcgaaggc gtcctccgcg
     1021 ctcaggttgg ggaggaagct cctcttgacg gcgtccttgc ccaggacggc ggagaagcgc
     1081 tccaggttga ggccgtggta gaggtcggag gcctcggcca tggagtccag ggacttttcg
     1141 taggcggcgg tgatggaggg attgtagaaa cgcgcctcca cgacgtcgaa gaggccggaa
     1201 aaggccgcga cggagaaggc cgcgaagacg agcacggcta tgaggaggct caggttggct
     1261 ttctggccga cggtcatcga atatccgccc tcgtcctttc cgttccagtt gccgcgggga
     1321 ggcccgtacg ggcgtccttg tcctctcgga ccgaaggcct aagacgccgc ttgggggggc
     1381 ggacgaaggg ccttccctct tctatcggaa ggaggaggcc ggctctcaac cgcattccgg
     1441 cgcggcgcgc gaagcgcggc gcgagccggc ccttccttga agaaggccca cgtccagaat
     1501 atactgctct ggtaatctac gccaaggagc ctcgatgcgt cttcgcgact tcagcctgaa
     1561 attcaaggtg cttttcatga cgatattcgg cgtcgccctc atcgccttcg tcatcgccgc
     1621 tttttgggtc aaagacatcg tggaaatggc gactcttggc atactggaga aaagccgcgg
     1681 tgtcgtcctc acggccgagg ccgcccgcga cgccatggcc gaccgactgg gcgcgggggt
     1741 catcaccgac ctggcccagc tggccgagga gggcgaccgg gagaagctcc tcaaggccgt
     1801 tcccatcctg acggccatcg aggtcgcggc gaagaacgcg gaggagagcg gctacgagtt
     1861 ccgcgtgccc aagttctccc ccaggaatcc cgccaactcc cccgtgggcg tcgaggtcga
     1921 ggcgctcaag aagctcgagt cgggggagct cgcggaatac gtggtgaggg aaaagggcca
     1981 gatccgctac ttcaggccca tccgcctcac cgaggactgc atgctctgcc acggcgagcc
     2041 cgcgggcgag cccgatcccg tgggcggcgt gagggagggc tggaaggtcg gcgagatcca
     2101 cggcgccttc gagctcgtga gcagcctgcg caaggcggag aaggacgcgg cgaaggcaga
     2161 gctcaacatc ggcggcctcg ccctgggcgt catggtcctc ctcggcctcg gcctcttctt
     2221 cgtcatcagg atcgtcctca agcccctcgg acactacgtg gagaccttca agaaggcggc
     2281 cacgggcgac ctgaccgcca gggccgacgc ccgctcccgc gacgaggtgg gccgcgtggc
     2341 cggcttcttc aacgacttca tcgccagcct ggagtccatg gtcgacgagg tgcggacggt
     2401 cgcggaaagc gcgaccgcca tctcccagga cctggccgcg agctcggagg agacggccgc
     2461 cagcctccac gagatcaggg ccaacaccga gggcatgaag gacaagatcg tccgcctgga
     2521 cgccgaggtg ggcgagagcg cccgctcggc ctccgacgtg cgggagttca tcgcccggct
     2581 ctccgagctc atccaggccc aggccgccgc gatcaccgag tcgagcgcga gcatcgagga
     2641 gatgagcgcc tccatcgcca acatcgcgaa gtcctccgag gagaagctca ggatcgcgaa
     2701 cgagctcgag gtctccgccc tggacggcca gtcggagatg gaggagaccg agcaggtcat
     2761 caagaaggtc gccgactccg cgaccgtgat catggagatg atccagatca tccaggacat
     2821 cgcctccagg accaacctcc tcgcgatgaa cgccgccatc gaggccgccc acgccgggga
     2881 gttcggcaag ggcttcgccg tcgtcgcgga cgagatcagg aacctcgcgg agtccagcgc
     2941 cgagagcgcc ggcgcgatca ccgactcgct gggcgaggtc gccgagtaca tcaaggtctc
     3001 cgagacgagc accgcgagga ccggcgaggt cttcagcagc atcgtcgccc agatcaagga
     3061 cgtctccgcg agcatgtcgg agatgaagaa cgccaccttc gagctctcca tcggcgcgaa
     3121 gcagatcctc gaggccctgg gctccctggt ccagacgacg gaggaggtga agaactcctc
     3181 ccgcgagatg aacacgcgcg tggccggcat cacggaggcg atggaccggg tgtcgggcat
     3241 cgcctccgac acgaggaacg gcatggagga ggtgacgatc ggcatcacgg agatcttcaa
     3301 ggcggcggag gcgatagccg aggccggcac caggaactcc gagtccgtgc gcagcctcaa
     3361 ggagctcgtc ggccgcttca ccctggccgg gcgggactag ggggcggagc ggagagcggc
     3421 gccgtggacg cgctggagcg cttcaccgag gaggccgcgg accgcctccg cgcggagatc
     3481 gaggaagcgg gcggcaacga ggtcttcgcc gcctgcaggc tcgacgccga ggggaggatc
     3541 tccgaggtcc tggtcgtcgc ccgcggccac gagagcgccg tgcccgcgct cgcgccctac
     3601 atcgagcgcg gcgacatcgt cgtccacaac cacccctcgg ggaccctgcg gccgagcgac
     3661 gcggacgtga acgtcgcggc ggaggcgggc tcgaagggcg ttggctccta catcgtcgac
     3721 aacggagtgc ggagcgtcta cgtcgtcgcc gaggcggcca ggcgcaaggc ctacagcctc
     3781 ctcgacgagg acgagcttgc gggcgtgctg gaggccgggg ggaagctctc caccctcatc
     3841 ccgagcttcg agccgaggcc gagccaggtg cgcctcgcgc gcgacatagc ccgcgcctac
     3901 aacgaggagc gggtcctcgc ggccgaggcg ggcaccggcg tcggcaagtc cttcgcctac
     3961 ctcatcccct ccttcgcctg ggccgtcaag aacgaggagc gcgtcgtcgt gtcgaccgcg
     4021 acgatcaacc tccagaagca gctcgtcgac aaggacatcc ccgtcgtcca gcgcctcttc
     4081 cggaagaaga ccaaggccgt cctcgtgaag ggccgcggca actacctctg ccgggtccgc
     4141 ctccgcgagg ccctggacga ggagggcctc ttcgcggagg agggctcgcc tctcaaggcg
     4201 atcgccgcct gggcggagac gagcgcgacg ggcgaccgga ccgacctctc cttctggccc
     4261 gaggactcac tctggtaccg cgtcgcctcg gagtcggagt cctgcctcgg cctccgctgc
     4321 cccgaccggg agacctgctt cgtcctccgc gtccggcgcg aggcggccga cgcccaggtc
     4381 atcgtcgcga accaccacat cctcttctcc gacctcgcgg cccgcatgtc gggcgcgggc
     4441 tacgaggcga ccgccgtcct cccgcccttc cgcatcctcg tcatcgacga ggcccacgcg
     4501 gtggagtcga gcgccacgag cttcttctcc gaggagctcg cgcgcttcgg cgtcaacaag
     4561 cgcctctcgc gcatactgcg caaaaggggc ggccgcagct tcggcatcgt cgagcgactc
     4621 ctcgccctcc cctccttccc gcgctcccgc ctggacgagc tcccggcggc catagcccgc
     4681 gcgcgggagg cgatggacgc cctggacaac aaggccgtcc tcctcttcga ccgcgagcgg
     4741 cagtacaggc tgaccgagcg cagccccctc ctcgaggact cgctcctaag gcccctgggc
     4801 gagctcgaga ggcgcctcct cgcctgcgcc gagatcctcc gcgaggccct ggatgaggcg
     4861 cccgaggcct ccgccggcga gcgcgccgtc tacgaggcca ggctcatgct caggcgcctc
     4921 gccgaggacg cggccctctg cgccaagttc aaggacttcg agtcgaaccc cgagaaggtc
     4981 ttctggctcg agaaggcgcg caccggccag ggagagacct tcgtccgcta cacggcgagc
     5041 cccctcgaca tcacgggcac gatggaggag gcggtcttca cgaagttccg ctccgtcgtc
     5101 tgcgcctccg cgaccctctc cgtcggcgag accttcgact tctggaagag ccgcgtgggc
     5161 ctccggcaca ccgacgtccc cgtggacacg gccgtctacg cctcgccctt ccccttccgg
     5221 acgaacgccc tcctcgcggt cgacgtctcg gcgccggggc ccgagagccc ggcctacaag
     5281 cgctacgtgg acgaggccgt cgtcgagctc ctccgcgcct cgcgcggccg cgccctcgtc
     5341 ctcttcaccg cctacgaggc cctgaagagc gcctacgaga aggccaagcc cgcgatggag
     5401 gagctgggca tcgcctgcat gcgccagggc gagggggagc gcggcaggct cctggaggcc
     5461 ttcaaggccg acgtctcgag cgtcctcttc gcgacggact ccttctggga gggcgtcgac
     5521 gcgccgggcg agaccctctc cctcgtcgtc atcgcgaagc tccccttccg cgtgccgacg
     5581 gatcccgtcc agctcgcgcg gagcgaggcc tgcgagaagc gcggcggcaa cgccttcatg
     5641 gagatcagcc tccccgaggc ggtcatcagg ctcaagcagg gcttcggccg cctcatccgc
     5701 cactcggagg acaggggcgc cgtcgtcatc ctggacagcc gcctcgcgac caagcgctac
     5761 gggccgctct tcgtcgcgag cctgcccgaa tgccgcctcg tcgcggagcc cctcccgaag
     5821 atcctctccg aagtggcaaa gttcctcgac gactggtagc attcgtcgat tgcctgcccg
     5881 cggaaagccg tgtagactgc ttgccagcat gcgcatctac agcctcgaga acccgatcca
     5941 gcgctacgac tggggctcgc ccgacggcct cgcctcggcc ctgggcatcc ccaatcccga
     6001 gggaggcccc gccgccgagc tctggatggg ggcccacccc aaggcgccct ccctggccct
     6061 ggtcgagggg aggcgcgtcg ccctggacgc cctcgtcgcc gccgagcccg aggccgtcct
     6121 gggacccgcc tccgtcgccc gcttcggccc cgccctcccc ttcctcttca aggccctctc
     6181 cgccggggcg ccgctctcca tccaggccca cccggccaag cgcaaggccg agaggggctt
     6241 cgagcgcgag aacctcgcgg gcatcccccg ggacgcgccc gagcgcaact accgcgacgc
     6301 gaaccacaag cccgagatgg cggtggccct cacccgcttc gaggggctct gcggcttcag
     6361 gcccgtggag gagatcgtcg agaacatccg cctcctctcg ccggacggcc actcccgtat
     6421 agcggggcgc ctggagcgca accccgggag agtcgagctc tccgtcctct tctacagcac
     6481 gatctccgcc ccggaggagg acaagcaggc cgccctggcc cgcgtgcggc cgcgcgtcga
     6541 gcgcctcctc gcggagggcg gcctcggccc ggcgagggag aaggccctcc gctgggccaa
     6601 gaggctcttc gagctcttcc cgggcgacat cggggccctc gcccccctcc tcctcaacct
     6661 cgtggccctg gagcccggag aggccgtctt catcggcgcg ggcgagctcc acgcctacct
     6721 cgagggcacg gccctggaga tcatggcgaa ttcggacaac gtgatacgcg gcgggctcac
     6781 gcggaagcac gtggacgtgc ccgagctcat ctccgtcctc accttctccc cggccactcc
     6841 cgtcgtcttc ggacccgagc gcgtctcgga gtccgaggag ctctaccccg ccttcgcccc
     6901 cgacttccag ctctcgcgcc tgaggccccg gcgcggctcg cccctgcggc gctcgcccct
     6961 cggccccgag atcctcctct gcggcgaggg cgccttcgcg atcagggagg gcggcggcgc
     7021 ggtcgagctc tcccgcgggg catcggcctt cgtgcgggcc gacgccgagg agtacgagat
     7081 cgagggagag gggacgctct tcgcggcctc cgtcccccgc ggcgggcggc ccgaggagcc
     7141 gtgaggatct acgcggacgg cgacagcctc caggcggagc tgcgcgtcct cctttcgcgg
     7201 cgggccgccg ccgagcgctc ccgcgccgcg gccagggacg aggagccccg cttctccctc
     7261 ctcttcgtcg ccgcccggaa gctcccgacg gcgggcggcg cggagctcct cctcgtggag
     7321 ggcgggccgg acgcggccga cggcaagatg ctcgagctgg ccctgcccgg cgacctcgcg
     7381 gtcacgcggg acctgcccct ggccgagcgc ctcgcggaga agggagtctc cgtcctcaac
     7441 gaccgcggcg aggccttcac ggcggagaac gtgcgggaaa ggcgctcgct gcgcgaccgg
     7501 gcggccgagc tccgcgccct gggcctggcc cccgagtcgc cccgcgcgcg gagctggggg
     7561 cagaaggagc tcaaggcctt cgccgacgcc ttcgaccgcg agctgacaag gctcctcaag
     7621 ggcggcacgg gcccgcgcta gaaggcgggc ctgagcctcg cgcgcgggtc ggcggcgcct
     7681 cgagtcgcgc cggcctctcg agccgcgcgc ccacgcagac atgagcctag ttctcgaggt
     7741 agcgcttgga gaggaactcg tagcgggagc gcaggatgcc gttcttggcc agcgtcgagg
     7801 gcttgtactt gatgggcgag gagaggaggg cgacgagccg cgccgcctcc tcgcgcgaga
     7861 gcctcgagag ggacttcccg tagtaggcgc gggaggccgc ctcgacgccg aagacgccct
     7921 tgccccattc cgcgtagccg aagtagagct cgaggatcct gtccttcggc aggagcagct
     7981 cgagctccag ggccgcgatc acctcgaggt acttgcggag gtagctcttc tccggcacga
     8041 ggaagagggt ccgcgccacc tgcatcgtga gcgtggagcc gccgtagagg gggcgcttga
     8101 tgcgcgagtt gatctcgtag gcccgctcga aggcctcgaa gtcgaggccg tggtgctcgt
     8161 agaacttgta gtcctccacc gacacgagca tcctccgcac gtaggaaggc accttcttga
     8221 gggccacggg ccgcggcggg accagcttcc agccgtagcc ccagctccgg taggccatga
     8281 ggacggtcac cgacgggtcg gagaagcggt agacggcgat gaggagggat gtggcgagga
     8341 cgtagccgag ctgggccagg aggaggccct tgccgaccgc gcggaggatc ctccccgggg
     8401 aaaggcggcg gcgcggcggc gcggccggcg gcagcctgag ctccggcggg agggcgccga
     8461 tccagggccc ctccgcgagg acgaggccgg gcacggccct ggccatcttc gcttcgagga
     8521 gggcttggcc tgcgaggctt tcggggcgcc gacggtaggg aatgccgtcc gtaggtgggg
     8581 gaaacataga caggccacgc tagcacggat ggccgccgag ggacaaggcg gctttccttg
     8641 acaacgattt tcgagcggtc tagtatgtcc tcgccggcgc cgcccggacc caccgaagag
     8701 gagaagctgc atgaaaaggt tcctgctcgc gctgtcctgc gccctcgcgc tcacaggctc
     8761 cctcttcgcc caagccacga gccatccggg actcttccgc tacaggctcg acaacggcct
     8821 cgagctcttc gtctaccggg acgcgaccct ccccctcgcg cgggtcggca tgtccttccg
     8881 cgcgggctcc tccttccagg ccgccgactc ggccggctcc ttcggcctcc tcgagcggct
     8941 cgccttcgag aacggagggc aggcgggcgg ccccgcgggg gcctcgcccc ggctcaaggc
     9001 cgcgatggcg cggatcggcg ccgcggagtc gggcggcggg accggcaccg actcgatggg
     9061 ctactggctc aggctgcccg cggccaggac cggcgaggcc ctgcgcttct gggccgagcg
     9121 cttcgtcgac gcgcccctcg atcccgccgc cctcgcggcc gcgaaggccg gcgccctcgc
     9181 ccggctcgcc gagctcaagg ccgagcccga cgccgtctac gaggccgccg tggcgaaacg
     9241 cctcttcggc aagtacccct ggaggcgcga ccccgccggc tccgaggcgg ccatcgcggc
     9301 cgccacgccc gagtccctcg cgaggctccg cgacgcatac ctcgttccgg gcaacgcggc
     9361 ggtcttcgtc ggcggcgacg tcgaccccga ggagacgcgc agggccgtgg aagccctctt
     9421 cggcggctgg aagagggccg acgaggcctc gcgtccggcc ctccccgccc accccaggcc
     9481 cggcgtgccc aggcccacct ggatcgtcca tcccgacgcg tccctccccg agggcctcgc
     9541 catggtcgag gcgcgctacc gcggccccga cctccagtcc gacccccagg gcagctacgc
     9601 ggccgacctc tggacggccc tggtcgccga tcccgagggc cgcttcaaga aggcggtcgc
     9661 caaggaggtg cccaagctct acggcaggga ctcgatctcc gcctactacc tgagccagcg
     9721 ggacggcggc gccctctccg tctccgccta cttctccgtc gatcccgccc tctcctccgt
     9781 cgaccgggcg cgccagttca aggagcgggt aaggggcttc gagatgaccg cgatgcggac
     9841 cgagccggcc tacttctccg cggccgacta cgaggcggcg aggcggcgcc tctcggacgc
     9901 gcgggccctc tccctcgaga ccgccgacgg cttcctcgag tcgctcgcct tctggtgggc
     9961 ctcggcctcc gccgactact tcctgggcta tcccgaggcc ctcgcgaaga cgggtccccg
    10021 cgaggtcggc gccttcatcg acgcctacgt catgcgcaac ctggaggtcg tggccctccg
    10081 gatgagcccc gcggactacg agcgggagaa gaagagcttc gccggcgcgg gcttcgaggt
    10141 cgtcacggcc gcgaacgcct tctggtggca gcgctgagcg cgggccgcgg aaggcgaggc
    10201 actgcccgcg ccctcgcggg ctgcgcggcc ggcgccgccc tcctcttcgc ctcctgcgcg
    10261 acgggcgccc ctcccgcggg ggaagcgggc ctccgccagg ccgcccctgc cggctccgcc
    10321 tccgccgcgg cgggagaggc ccgccccgcg gaccggcgcc cccggcagag cccctatctg
    10381 ctggccagcc tggcctccat cgagcgcgaa aggctcgcta gcggcgtcgt cctcctcgca
    10441 aggaggctgc ccggccgcgg cgtcgcggcc gcggagctcc tcctcctccg cgacgggtcg
    10501 acgccctcgg gcaaggacgg cgtcgaggcc ctggccctct ccctcgcagc caggggggga
    10561 aggcttccct cgggcgggcg cgccgacctt cctgccctcg tccgggccgc gggcggaagc
    10621 ctcgagctca aggcgctctc ggaggacgcc ctggccctgg agctcgccgc gcccctggac
    10681 cgcctgcccg ccctcctaga ggccctggcc gcctccgtgg ccgcaccctc cttcgaggag
    10741 gcggacttcg tggccgcgat gcgcgacctc cgcgcggcca ggctcgcggc ggaggccgac
    10801 ccggcccgcg tcgcggcgga gggcttacgc accgaggcct accggggaag cccctacgcc
    10861 cggccgccct tcggcacggc cgagagcctc gccgcgctcc gcctggagga cgcggccgcc
    10921 tattggcgcg aggccgtcgg cgccgagcgc gtggccctgg tcgtcgccgg cgacctggga
    10981 gcggccgagc tcgcggcccg cgtcggcccg gccctggcgg ccctcccgcg gaagggcgga
    11041 gccgccgggc tcctccccga gcccggctcc gcgccggcca t
//
LOCUS       DAOOSJ010000060        10944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3930_length_10944_cov_4.894940, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000060 DAOOSJ010000000
VERSION     DAOOSJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3930_length_10944_cov_4.894940"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLG14_04485"
     CDS             <1..855
                     /locus_tag="PLG14_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOX48423.1"
                     /translation="VSAFCNAGFSTFPDSLESRVSDPVVNLVVMSLIVAGGIGFTTLR
                     DIGKRATGSKRRLSTQTKVVLSTTAVLLLGGAAVYYVLEAGHAYRSMPPGERLMAAAF
                     QSVTPRTAGFDTVPQSRLSDASVLVTMILMFIGASPGSTGGGVKTTTLFIVVLAALRG
                     VDEKSRLAVGRRAIPTTTILKAFSIIGKAVLLVVASATALLAMERHAVVSGDLGLVEV
                     LFESISAFGTVGLSLGITARLSAAGKLVLVLTMFAGRVGLFAMALPKPRGSLERYVDL
                     PSTDMMTG"
     gene            865..1602
                     /locus_tag="PLG14_04490"
     CDS             865..1602
                     /locus_tag="PLG14_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOX48424.1"
                     /translation="MRQFAIIGLGSFGTRMLEKLSEITDELIILDKDADTIERYKELA
                     GNAYVVDAIDDEALRRVLPEGLDAAIVDMGGNIEAAILVTNSLKKLGVREIIVKADSE
                     ERGEVFSLVGATRVVYPDKEAAARIVPLLVSPSLFNFMPIGANLVMAEVRIPENYAGM
                     SLIEANLRQRHGINVIALRSESSQEYRFFSADYKLQADDILLVAGKEREVIDFSGVEE
                     RKARGGLGDIFAGLLMGKGSEKHGKKK"
     gene            1586..1711
                     /locus_tag="PLG14_04495"
     CDS             1586..1711
                     /locus_tag="PLG14_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48425.1"
                     /translation="MGRRSETPAPAGTIGKERRLASEPGTAAVVLIGSYIAAQII"
     gene            1739..2263
                     /locus_tag="PLG14_04500"
     CDS             1739..2263
                     /locus_tag="PLG14_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOX48426.1"
                     /translation="MLGLAIDAGTFIYPITFTLRDLVHKRLGRRAAREAVLLAGALNV
                     FMALYLQLVAQTAPDPTWPLNEAFVAVLGPVWRIVVASIIAEVVGELLDGEIYHLWTA
                     RFPGAPQWTRVLVSNAVSVPLDSLVFCWGAFAFTMPSATVWAIVGSNILMKAAVTLLS
                     LPGIYLVKQGDAAQ"
     gene            2640..3818
                     /locus_tag="PLG14_04505"
     CDS             2640..3818
                     /locus_tag="PLG14_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOX48427.1"
                     /translation="MDIFKKCREFDADKKAMEKGIYPFFQPLEGTEGTEVTMNGRKVL
                     MLGSNNYLGLTTHPKVREAAKKAIDTFGTSCTGSRLINGTLCIHEELEHRLAAFMRRE
                     AALVFSTGMQTNLGSISCLIGRNDIVVTDKEDHASIVDACKLGYGEVKRFVHGDLEQL
                     ERILEKIPEDKGILVVVDGIFSMGGEIVDLPKLGPLCRKYGARLYVDDAHAVGVLGGG
                     RGTAVHFDQLKDVDLTMGTFSKSFASLGGYVVGDKDVVNYIKHTARSFIFSASLPAPS
                     VMAAMAALDIMESEPEHVERLWENAHFMMKGFRELGFNIGHTQSPIIPIIIGEDDKTF
                     MFWKVLYDAGVYTNPVCAPAVPQGMSLLRTSYMATHTREQLTKALGVFEKAGKELGVI
                     "
     gene            complement(4087..4791)
                     /gene="radC"
                     /locus_tag="PLG14_04510"
     CDS             complement(4087..4791)
                     /gene="radC"
                     /locus_tag="PLG14_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX48428.1"
                     /translation="MKSDALYDSARPEGEAGKPRARERLSRQGPASLSDGELLSVLLG
                     TGARGKGVSRLAEEVLDLLEAARSSPDAASLRGLEGMGEAKSCAVAAALELGRRLYGL
                     RDKRIASPRDVYPLVAHWADRKQERFVCCSLNGAHEILAVRVVSVGLVNRTVVHPREV
                     FADPIVDRACAVIVGHNHPSGRLEPSPEDLEITRRLKEAGELLGLVLLDHLIFSREGF
                     YSFVEHGLLEAPPPES"
     gene            5062..5636
                     /locus_tag="PLG14_04515"
                     /pseudo
     CDS             5062..5636
                     /locus_tag="PLG14_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
     gene            5671..6057
                     /locus_tag="PLG14_04520"
     CDS             5671..6057
                     /locus_tag="PLG14_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOX48429.1"
                     /translation="MAELMKVYKCELCGNIVEVLHAGKGDLVCCGQPMKLLAENVTDA
                     AKEKHVPVVEKTAEGWKVTVGSVLHPMEPDHYIEWIELVVGQAEGGEVHRKHLKPGDR
                     PEALFCVKASSVSAREYCNKHGLWKA"
     gene            6074..6232
                     /locus_tag="PLG14_04525"
     CDS             6074..6232
                     /locus_tag="PLG14_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOX48430.1"
                     /translation="MKKYVCDVCGYIYDPAVGDPDSKVKPGTAFESLPADWVCPMCGA
                     GKDSFSPA"
     gene            6336..6626
                     /locus_tag="PLG14_04530"
     CDS             6336..6626
                     /locus_tag="PLG14_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48431.1"
                     /translation="MISREDFAFTIGYDGLHAVVDGKAKRQYGSLSTMELAEKGLYRA
                     AFSSALHSGDEAEMRAFIGYFNGKAGTAYKTADELKRLFGVNHEEVKKVLVL"
     gene            complement(6676..7914)
                     /locus_tag="PLG14_04535"
     CDS             complement(6676..7914)
                     /locus_tag="PLG14_04535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOX48432.1"
                     /translation="MDRARIILRPKEDLRLRAGHPWVYDNEIARVEGEPEPGAAVDVF
                     DSRLQAVGSAFWNPASKIRCRIYSRAPRAADEAFFAEAFGRALAWRRRFFDPERQSLR
                     LVFGEADGVPGLVVDSFVGRPDGGAAGAAPDGPASRWLSAQFLSLGVERRKAEILAAL
                     DKVFPSAGVAERSEAPVRKYEGLPPESGVVAGTVPPRIVMEENGLAFGVDILEGQKTG
                     WFLDQRSNRAAAARFAKGRRVLDAFCNAGGFGLACAAAGAESVLAVDASAEAVAAVSA
                     NAARNGLGGLVKAEEANAFDFLRGLERSKERFGLVVLDPPAFAKNRASVEGAHRGYKD
                     LNLRALKLLEPGGVLVTCSCSHWFGPERFSSMLEEAARDAGRRLRLVEERTQDLDHPI
                     VSGYGESRYLKCMIVEAMER"
     gene            8116..10083
                     /locus_tag="PLG14_04540"
     CDS             8116..10083
                     /locus_tag="PLG14_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOX48433.1"
                     /translation="MEARAFSRRGTSSLRLAAAAVLALAAALALAACAAAPRPAATGA
                     VGASGGAASSGPALGGETAAKPAEPPAPALSPATADLKRSEEATRGADKTAASKDDEF
                     GGFALREEKGGLAAAAPSSALEGASRGSGGEARTAPSQSGLKAGFSDDNEQYGLFVKF
                     LDEFKETPHRELDVRERIVVKALDAKDRPVANAEVLVKAGGRTLARGRTYSDGSFSLY
                     PREYAAAADAAAYRVEISSPAGKAVLDLSRAGQRSVAARLEAERKIPAPLPVDVLFVM
                     DTTGSMGEEIERLRATIEIIHTNLSALKPRPAVRFGLVLYKDREDEYLTEVHPFTADL
                     EAFQKVLDGVTAGGGGDTPEDLESALRDAVRGMAWNKDGLRLAFVVTDAPPQAYEDAG
                     YTYAQAAREAKEKGVKLFTIGTGGLPLEGEYVLRQVAQYTQAKYIFLTYGEGGEAEGG
                     KEGSVSHHTGTNFTTDKLEAVVIRFVKDELSRFSEKPLAYDESFYEANKIADESREDT
                     LGKLFAEALRNLADYSTYRLGPETRLAVMPVAPPPASEGAAPDPALAANAEYFGAQLG
                     AAAAASKLWTVVERKDLQAILKELELQLSGLADEDSAAKVGKLLGAEILVSGSVYRKG
                     DRYELFLKLVRVETAEVLAATKAKIDLKLGL"
     gene            complement(10265..>10944)
                     /locus_tag="PLG14_04545"
     CDS             complement(10265..>10944)
                     /locus_tag="PLG14_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48434.1"
                     /translation="PGQAVERREGARLRRLAVAAGEAEGFRQRWAEEEAAAFRRDAPR
                     AAKAIAQRYLELQAAFAESQEKAAGSEVLRRWIAERKAGSKGGRPDLAALERDLRELR
                     KLGPSLLEGRRLLGQLERANAAVAALEEALAGDEAVLKQEIRKGITVGDFFGKFAAER
                     ERLDRQVAWFRQAGKLLKQREGLLERARSGGPGGTPPPPPPGAPGGAPPPPPPGAPGE
                     APPPPKP"
BASE COUNT         1737 a         3876 c         3751 g         1580 t
ORIGIN      
        1 gtctccgcct tctgcaacgc cggcttctcg acctttccgg acagcctcga gtcgcgcgtc
       61 tcggaccctg tcgtgaacct ggtcgtcatg tcgctcatcg tcgcgggggg catcggcttc
      121 acgacccttc gggacatcgg caagcgcgcg accggctcga agcggcggct ctcgacgcag
      181 accaaggtag tcttgtcgac gaccgccgtc ctcctgctcg gaggagccgc ggtctattat
      241 gtccttgagg ccggccacgc ataccggagc atgccgccgg gagagcggct gatggcggcg
      301 gccttccaat ccgtgacccc caggacggcg ggcttcgaca cggtgccgca atcacggctt
      361 tccgacgcat cggtcctcgt cacgatgatc ctcatgttca tcggcgcctc tcccggctcg
      421 acaggcggcg gcgtcaagac caccaccctc ttcatcgtag tcctggccgc cttgagggga
      481 gtagacgaga aatcccgcct ggccgtcggc cgccgggcga tccccacgac gacgatcctc
      541 aaggccttct ccataatcgg gaaggcggtc cttctcgtcg tggcctcggc cacggccctc
      601 ctggccatgg agaggcacgc cgtggtctca ggcgacctgg ggctcgtcga ggtgctcttc
      661 gaatcgatat cggccttcgg caccgtcggc ctctccctgg gcatcaccgc caggctttcg
      721 gcggccggaa agctcgtcct cgttctcacg atgttcgccg gccgcgtcgg cctcttcgcg
      781 atggccctgc ccaagccgcg cggaagcctg gagcggtacg tcgaccttcc ttcgaccgac
      841 atgatgactg gataggagaa agcgatgcgc caattcgcga tcatcgggct tggaagcttc
      901 ggcacgagga tgctcgagaa gctgtcggag atcaccgacg agctcatcat cctcgacaag
      961 gacgcggaca cgatagagag gtacaaggag cttgccggca acgcctacgt cgtggacgcg
     1021 atcgacgacg aggcgctccg ccgagtcctc cccgagggcc tggacgcggc catcgtcgac
     1081 atgggcggca acatcgaggc cgctatcctc gtcacgaaca gcctgaagaa gctcggcgtc
     1141 cgcgagatca tcgtgaaagc cgacagcgag gagcggggag aggtgttctc gctcgtgggg
     1201 gcgacgaggg tggtctatcc ggacaaggag gccgccgcgc gcatcgtgcc cctcctcgtc
     1261 tcgccctcgc tcttcaactt catgccgatc ggcgcgaacc tcgtcatggc cgaggtccgg
     1321 atcccggaga attacgccgg gatgagcctc atagaggcga acctgcgcca aaggcacggc
     1381 atcaacgtca tcgcgctgcg ttcggagtcc tcgcaggagt accggttctt cagcgcggac
     1441 tacaagttgc aggccgacga catactcctc gtggccggca aggagcgcga ggtcatcgac
     1501 ttctcgggcg tcgaggagcg gaaggccagg ggcggcctag gcgacatctt cgcgggcctc
     1561 ctcatgggga aaggaagtga gaagcatggg aagaagaagt gagacgcccg ctcccgcggg
     1621 tacgataggc aaggagagga ggctcgcttc ggagcccggc acggcggcgg tcgtgctcat
     1681 cggatcctac atcgcggccc agatcatcta ggacatcgga agcctgaaga tcacgaccat
     1741 cctgggcctg gccattgatg cgggcacctt catctacccg attaccttca ccctgcgcga
     1801 cctcgtgcac aagcggctgg gccgcagggc ggcccgcgag gccgtgctcc tggccggcgc
     1861 cctcaacgtc ttcatggccc tgtatctcca gctcgtcgcc cagacggcgc cggatcccac
     1921 ctggcccctc aacgaggcct tcgtggccgt gcttggcccc gtctggcgca tcgtcgtcgc
     1981 gagcatcatc gcggaggttg tcggcgagct cctggacggc gagatctacc atctttggac
     2041 cgcgcgcttc cccggagccc cccagtggac gagggtgctc gtctccaacg cggtgagcgt
     2101 ccccctggac agcctggtat tctgctgggg cgccttcgcc ttcacgatgc cttcggccac
     2161 ggtgtgggcc atcgtcggct ccaacatcct catgaaggcc gcggtcaccc tcttgagtct
     2221 cccgggcatc tatctcgtca agcaaggcga cgcggcccaa taggccgggg ccgcgcggcg
     2281 ggccgttccc gcgatgcgcc gggccgcttc ctcggtctcg gcctaagtct cggcctcgga
     2341 tgcagtcttg gcgctcgcgc ccgtccggca atccagcccc gtcttcaaga aaagacgggg
     2401 cttttttgtt gtttcgtgcc gttcgccgca ttacttgacc tcgccgaatg gagcgggcta
     2461 tactttgaca ttgcgggacg aaacgtcgaa ccacggcggc gccccgctac ggagccgcga
     2521 gggccgagga gacatactcg ctcggacctc gcgaagcggc tcccttccga cggcgccctt
     2581 gcgcggcgcc aagcatctcg ccatcgcgcg gacctagccg cgcgacaggg ggttcttcca
     2641 tggacatctt caagaagtgc cgcgagttcg acgcggacaa gaaggcgatg gaaaagggca
     2701 tctatccctt cttccagccg ctcgagggca ccgaaggcac cgaggtgacc atgaacggcc
     2761 gcaaggtcct catgctcggc tcgaacaact acctggggct cacgacgcat cccaaggtcc
     2821 gcgaggccgc caagaaggcg atcgacacct tcggcacctc ctgcacgggc agccgcctca
     2881 tcaacggcac cctctgcatc cacgaggagc tcgagcaccg gctcgccgcc ttcatgcggc
     2941 gcgaggcggc tctcgtgttc tccaccggca tgcagacgaa cctcgggtcc atctcctgcc
     3001 tcatcggccg caacgacatc gtcgtcaccg acaaggagga ccacgcctcc atcgtcgacg
     3061 cctgcaagct gggctacggc gaggtgaagc gcttcgtcca cggagacctg gagcagctcg
     3121 agcgcatcct cgagaagatc cccgaggaca agggcatcct cgtggtggtc gacggcatct
     3181 tctcaatggg cggcgagatc gtcgacctgc ccaagctcgg ccccctctgc cgcaagtacg
     3241 gcgcccgcct ctacgtcgac gacgcccacg cggtcggcgt cctgggcggc ggccgcggca
     3301 cggccgtcca cttcgaccag ctcaaggacg tggacctcac gatgggcacc ttctccaaga
     3361 gcttcgcctc cctcggaggc tacgtcgtcg gcgacaagga cgtggtcaac tacatcaagc
     3421 acacggcgcg ctccttcatc ttctcggcct cgcttcccgc ccccagcgtg atggccgcga
     3481 tggccgccct ggacatcatg gagagcgagc ccgagcacgt cgagcgcctc tgggaaaacg
     3541 cccacttcat gatgaagggc ttccgcgagc tcggcttcaa catcgggcac acccagtccc
     3601 ctatcatccc catcatcatc ggggaggacg acaagacctt catgttctgg aaggtcctgt
     3661 acgacgcggg cgtctacacg aaccccgtct gcgcccccgc ggtcccgcag ggcatgtcgc
     3721 tgctccgcac gagctacatg gccacgcaca cgagggagca gctcacgaag gccctcggcg
     3781 tcttcgagaa ggccggcaag gagctcgggg tcatctaggc ccggcgcctc ccggcggacg
     3841 aagggccgcg cctttccccg gaaggggagg gcgcggccct tcgcttcatg gccaggcgct
     3901 ggaatgctgc cggcggaaaa cgcaagggag ctcctcgcgg aacgccccac ggtcttccct
     3961 ggcgaggcgc cgcgccggtc gcccggcctt tcctccaacc gggtatccat ccgggcgacg
     4021 gtccggcgag gctcggccgc cgcagcggag cgccggtcaa gccgacccgg ccgccgccgc
     4081 ggcggctcaa ctctccggcg gcggcgcctc gaggaggccg tgctcgacga agctgtagaa
     4141 gccctcgcgc gagaagatga gatggtcgag gaggacgagg ccgaggagct cgcccgcctc
     4201 cttgagccgc cgcgtgatct ccaggtcctc gggggagggc tcgaggcggc cggaggggtg
     4261 gttgtggccg acgatgaccg cgcaggcccg gtcgacgatc ggatcggcga agacttcccg
     4321 ggggtggacg accgtgcggt tcacgaggcc gacggagacc acccgcaccg cgaggatctc
     4381 gtgagcgccg ttcagggagc agcagacgaa gcgctcctgc ttgcggtcgg cccagtgggc
     4441 gacgagggga tagacgtcgc ggggggaggc gatgcgcttg tccctgaggc cgtagagccg
     4501 gcggccgagc tcgagggcgg ctgccaccgc gcaggatttc gcctctccca tgccctccag
     4561 gcccctgagc gaggcggcgt cgggcgagct gcgcgccgcc tcgaggaggt ccaggacctc
     4621 ctccgccagc ctggacacgc ccttgcccct cgcgccggtg ccgaggagga cggacaggag
     4681 ctccccgtcc gacagcgagg ccggcccctg cctcgagagc ctttccctgg cgcggggctt
     4741 tccggcctcg ccttcgggcc gggccgagtc gtagagcgcg tcgctcttca tgggcactcc
     4801 tcgacttgcc tacgcgcgaa tgcggggccg gcgcttgagg cgacgcatgg gtggtcgctt
     4861 gggacggcgg ggcgccaaag ccgggccgcg gcgagtttct ttctttcgtt gcagttcgcc
     4921 ttcgcagcta gtataattag ggcgtgcggc ggcggctttc tcgccgccgc gccaaacggg
     4981 atataacgcg cgccggggca gtccccggcg gccgcggcgc ctcgccgccg cggacctcga
     5041 tcccccaagg agagctgagc gatgaagagc gtgaagggca cgaaaaccga taccaacctc
     5101 atggccgcct tcgccggaga gtcccaggcg aggaaccgct acacctacta cgcctccaag
     5161 gcccgggacg agggcttcat ccagatcgcc ctggtcttcg aggagaccgc taaccaggag
     5221 aaggagcacg cgaagcggct cttcaagttc atggagggcg gcacgaccga gatcacgggc
     5281 tccttcccct tcggcgtcat cggctcgacg gtcgacaacc tcctccacgc ggccgagggc
     5341 gagaaccaca atggcagtcc atgtaccccg atttcgccaa ggtcgcccgc gaggagggcc
     5401 tggacgcgat cgccgccgtc ttcgaggcga tcgccgtcgc ggagaagcag catgcccgcc
     5461 gctacgagaa gttccggaag aacatccagg agggcaaggt cttcaaggcg gacaagccgg
     5521 cggtctggcg ctgcctcaac tgcggatacc tggccgaggg gcccgaggcc cccaaggcct
     5581 gccccgcctg cgcccacccc cagtcctatt tcgagctcct gggcgagaac tactgatcac
     5641 ctgaaatcca tccgtgaaag gaggcccaat gtggccgaac tcatgaaggt ctacaagtgc
     5701 gagctctgcg gcaacatcgt cgaggtcctc cacgccggga agggcgacct cgtctgctgc
     5761 ggccagccga tgaagctcct cgctgagaat gtcaccgacg cggcgaagga gaagcacgtc
     5821 cccgtcgtcg agaagacggc ggagggctgg aaggtcacgg tcggctccgt cctccacccg
     5881 atggagccgg accactacat cgagtggatc gagctcgtcg tcggccaggc cgagggcggc
     5941 gaggtccacc gcaagcacct caagcccggc gacaggcccg aggccctttt ctgcgtgaag
     6001 gcctcctcgg tctccgccag ggaatactgc aacaagcacg gcctctggaa ggcctgagaa
     6061 aaaaggagac gagatgaaga agtacgtgtg cgacgtctgc ggctacatct acgaccccgc
     6121 ggtgggcgat cccgattcca aggtgaagcc gggcaccgcc ttcgagagcc ttcccgccga
     6181 ctgggtctgc cccatgtgcg gggcgggcaa ggacagcttc tcgccggcct gaggccccta
     6241 ggcggcgacg gccgacaggg gccgcggatc ggcgatccgc ggcccctgca tcgctactac
     6301 ccgggccctg gcggcccgtg ggcatggaga ctatcatgat ttcccgcgag gatttcgcct
     6361 tcaccatcgg ctacgacggc ctccacgccg tcgtcgacgg caaggccaag cgacagtacg
     6421 gctccctctc cacgatggag ctcgcggaga aggggctcta ccgggccgcc ttctcctccg
     6481 ccctccattc gggcgacgag gcggagatgc gggccttcat cggctacttc aacggcaagg
     6541 cgggcaccgc ctacaagacc gcggacgagc tcaagcgcct cttcggcgtc aaccacgagg
     6601 aagtgaagaa ggtcctcgtc ctctagcgcc cggggcgccg cgcgcggccc gccgcggcgg
     6661 gccccgcgcc cggccctacc gctccatcgc ctccacgatc atgcacttca ggtacctcga
     6721 ctcgccgtag cccgagacga tcgggtggtc caggtcctgg gtgcgctcct ccacgaggcg
     6781 caggcggcgg cccgcgtctc gggccgcctc ctcgagcatg gaggagaagc gctcggggcc
     6841 gaaccagtgc gagcaggagc aggtgacgag gacgccgccc ggctcgagga gcttgagggc
     6901 gcggaggttc aggtccttgt agccgcgatg cgcgccttcc accgaggcac ggttcttcgc
     6961 gaaggcgggc gggtcgagga cgacgaggcc gaagcgctcc ttggagcgct cgaggcccct
     7021 gaggaagtcg aaggcgttgg cctcctcggc cttcacgagg cccccgaggc cgttgcgcgc
     7081 cgcgttggct gaaaccgcgg ccacggcctc cgccgaggcg tcgacggcga gcacggactc
     7141 cgcgccggcc gcggcgcagg ccaggccgaa gccgcccgcg ttgcagaagg cgtcgaggac
     7201 gcgccggccc ttggcgaagc gggcggcggc ggctcggttc gagcgctggt cgaggaacca
     7261 gcccgtcttc tgcccctcga ggatgtccac cccgaaggca agcccgttct cctccatgac
     7321 gatccgcggg ggcacggtcc cggcgacgac gcccgactcg ggcggcaggc cctcgtactt
     7381 gcgcacgggc gcctcgctcc gctcggcgac gcccgccgag gggaagacct tgtccagggc
     7441 ggcgaggatc tccgccttcc gcctctccac gccgagggag aggaactggg ccgagagcca
     7501 gcgcgaggcc ggcccatccg gggccgctcc cgcggcgccg ccgtcggggc ggccgacgaa
     7561 ggagtcgacg acgaggcccg ggacgccgtc ggcctcgccg aagaccaggc ggaggctctg
     7621 cctctcggga tcgaagaagc ggcggcgcca ggcgagggcg cggccgaagg cctccgcgaa
     7681 gaaggcctcg tcggcggcgc gcggggcccg cgagtagatc ctgcacctga tcttggaggc
     7741 ggggttccag aaggccgagc cgacggcctg gaggcgggag tcgaagacat ccacggccgc
     7801 gccgggctcg ggttcgccct cgacgcgggc tatctcgttg tcgtagaccc aggggtgccc
     7861 cgcgcggagg cggaggtctt ctttcggccg caggatgatt cgcgctctgt ccatgccccg
     7921 agggtacagg ggggagcctt ccgagcgcaa gcgggcgcgg gctctcggcg cctcccgcgc
     7981 cggcacgccg ccgcgcgagg ccgccgggaa gcccggccaa gcgggccgga ggccgtcaga
     8041 gggggcggcg gggcggaaag cgcggggaaa acgcactata ctggatgccg atccgtccag
     8101 cctgaaggag gcatcatgga agctcgcgcc ttttcccgcc gggggacttc gtccctgcgg
     8161 ctcgccgcgg cggcggtcct ggccctcgcg gcggccctgg ccctggccgc ctgcgccgcg
     8221 gcccctaggc cggcggcgac cggggcggtc ggcgcctcgg gcggcgcggc atcctccggc
     8281 cccgcgctgg gcggcgagac ggcggcgaag cccgccgagc ctcccgcgcc ggccctctcc
     8341 ccggcgacgg ccgacctgaa gcgctcggag gaggcgacga ggggggcgga caagaccgcc
     8401 gcctccaagg acgacgagtt cggcggcttc gccctccgcg aggagaaggg cggcctcgcc
     8461 gcggccgcgc cgtcctcggc cctcgagggc gcctcccgcg gctccggcgg cgaggccagg
     8521 accgcgccga gccagtcggg cctcaaggcc ggcttctccg acgacaacga gcagtacggc
     8581 ctcttcgtga agttcctgga cgagttcaag gagaccccgc accgcgagct cgacgtcagg
     8641 gaacggatcg tcgtgaaggc cctggacgcg aaggacaggc ccgtcgcgaa cgccgaggtc
     8701 ctcgtgaagg cgggcggcag gaccctggcc cgcggcagga cctactccga cgggagcttc
     8761 tccctctacc cgcgcgagta cgcggccgcg gccgacgccg ccgcctatcg cgtcgagatc
     8821 tcgtctcccg ccggcaaggc cgtcctcgac ctttcgcgcg cgggccagag gagcgtggcg
     8881 gcgaggctcg aggccgagcg gaagatcccc gcgccgctcc ccgtcgacgt gctcttcgtg
     8941 atggacacca cgggctccat gggcgaggag atcgagcgcc tgcgcgccac gatcgagatc
     9001 atccacacga acctctcggc cctgaagccc aggccggcgg tccgcttcgg cctcgtcctc
     9061 tacaaggacc gcgaggacga gtacctgacg gaggtccatc ccttcacggc cgacctggag
     9121 gccttccaga aggtcctgga cggcgtcacg gccggcggcg gcggcgacac gcccgaggac
     9181 ctagagtcgg cgctcaggga cgcggtccgc ggcatggcct ggaacaagga cggcctccgc
     9241 ctggccttcg tcgtgaccga cgccccgccc caggcctacg aggacgcggg ctacacctac
     9301 gcccaggccg cgcgcgaggc caaggagaag ggcgtgaagc tcttcacgat cggcacggga
     9361 ggcctccccc tggagggcga gtacgtcctc cgccaggtcg cccagtacac gcaggccaag
     9421 tacatcttcc tcacctacgg ggagggcggc gaagccgagg gcgggaagga gggctcggtc
     9481 tcccaccaca cgggcacgaa cttcacgacc gacaagctcg aggcggtcgt catccgcttc
     9541 gtgaaggacg agctctcgcg cttctccgag aagcccctcg cctacgacga gtccttctac
     9601 gaggcgaaca agatcgcgga cgagagccgc gaggacaccc tcgggaagct cttcgccgaa
     9661 gccctgcgga acctcgccga ctactcgacc taccgcctcg gccccgagac gcggctcgcc
     9721 gtcatgccgg tggcgccgcc gcccgcgagc gagggcgcgg cccccgatcc cgccctcgcg
     9781 gcgaacgccg agtatttcgg cgcccagctc ggcgcggccg cggccgcctc caagctctgg
     9841 accgtcgtgg agcgtaagga cctccaggcc atcttgaagg agctcgagct ccagctctcg
     9901 ggcctggccg acgaggattc ggccgcgaag gtgggcaagc tcctcggggc ggagatcctc
     9961 gtctccggct ccgtctacag gaagggcgac cgctacgagc tcttcctgaa gctcgtccgc
    10021 gtggagacgg ccgaggttct cgccgcgacg aaggccaaga tcgacctgaa gctcgggctc
    10081 taagggccgg gggccgcggc cagggcctcg cggctacggc gggcggccgg ttcccggtgt
    10141 cgagaaagga tgccggcggc cccgaagggc cgcggcggcc cggcgcgcgg cccgccgcca
    10201 ggaaccggga caggaagcga gcggccggcc cttgaagggc cggccgctct tgttatccgc
    10261 gatgtcaggg cttggggggc ggcggcgctt cgcctggagc gccgggaggg ggaggcggcg
    10321 gcgctccgcc tggggcgccg ggaggaggag gcggcggcgt cccgccgggg ccgccggacc
    10381 tcgcgcgctc gagcaggccc tcccgctgct tgaggagctt gcccgcctgc ctgaaccagg
    10441 cgacctggcg gtcgagcctc tcccgctcgg cggcgaactt cccgaagaag tcgcctacgg
    10501 tgatgccctt gcggatctcc tgcttgagga cggcctcgtc cccagccagg gcctcctcca
    10561 gggcggccac cgcggcgttc gcccgctcga gctggccgag gagccttcgc ccctcgagga
    10621 ggcttgggcc gagtttcctg agctccctga ggtcgcgctc cagggcggcc aggtcggggc
    10681 ggccgccctt cgatcccgcc ttccgctccg cgatccagcg ccgcaggacc tcggagccgg
    10741 cggccttctc ctggctctcg gcgaaggccg cctggagctc gaggtacctc tgggcgatgg
    10801 ccttcgcggc cctgggcgcg tcgcgcctga aggcggcggc ctcctcctcg gcccagcgct
    10861 ggcggaagcc ctcggcctcc ccggcggcga cggccaggcg cctgagccgc gcgccctcgc
    10921 gacgctcgac ggcctggccg ggaa
//
LOCUS       DAOOSJ010000061        10939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3931_length_10939_cov_4.766171, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000061 DAOOSJ010000000
VERSION     DAOOSJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3931_length_10939_cov_4.766171"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..730)
                     /locus_tag="PLG14_04550"
     CDS             complement(65..730)
                     /locus_tag="PLG14_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOX48435.1"
                     /translation="MLTYLLVALGLSMDAFAVSVSSGICIPHMKARHALRAAFAFGLF
                     QFLMPVAGWLAGSAFRAYIQGLDHWIAFALLALVGGKMLKESFELEEESACADPAPAA
                     AAAGSVGPGAATSVGPGGAEAAKPKAAKRSILDLGGLLVLAVATSLDALAVGLSYSML
                     GTPILGPAAIIGLVTFGLCLFGCEFGKRIGAKFERWAEVAGGVVLIGIGIKILAEHLV
                     KAV"
     gene            complement(775..1812)
                     /locus_tag="PLG14_04555"
     CDS             complement(775..1812)
                     /locus_tag="PLG14_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX48436.1"
                     /translation="MTEFWQDSPEIAASLEKVAALMDQSVRGSDFPLEEAVASIVASN
                     GKMLRPALILIGAGFGRRGYEERILPLAAAVELLHVSTLIHDDVIDEAELRRGVPTIH
                     SRFGAKEAVLAGDWLFARCFLLAAESAGPGNAKALARLITAICAAEIRQDLDKGSYSP
                     SVRSYLRKIAGKTAALFSLSLHVGATESKAPPPVAERLRRAGYDIGMAFQVIDDILDF
                     ESSEGTMRKPVGRDVAEGLCTLPLIHALRADGAGMRALLSRLPRPGRADRAEAGAREE
                     ALARGDAVARDEAVAAILARASELGGVEAARRDAARFTARAQAEIARLPRCEAREELA
                     RVADKLLSRKY"
     gene            complement(1941..3902)
                     /locus_tag="PLG14_04560"
     CDS             complement(1941..3902)
                     /locus_tag="PLG14_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48437.1"
                     /translation="MGDKKRILILGGGYGGVWAGKILEKRFRKRDDVEITLVDMKPYH
                     TLMTELHEVAGWRTEPESVQVSFRKIFGAKRIEVATDKILKVDFEAKKAFSALREYPF
                     DYIVVGAGAEPEYFGIPGVQENSFSLWSFEDAMRIRHHLEETFAKAAAEPDAAKRRRL
                     LTFVVAGAGFTGIEMIGELLEYRDTMCRKHFLDPKETRVVCVEALPSILPILEEPLRA
                     KAEGYLKKAGCEIMLGSAIIGAEPGKVLLKDGGVLETDTFIWTCGVKGSSFAGSLEVP
                     MGKRNRIECDEEMKSTKYPFVYVVGDNAGLIRDGKPLAQIVETAHFTAEAAAKNIIAD
                     IDGGERHKFTPNYHGVMVCIGSHVGVSNAGNMKTTGWFALFIKHFINVYYLFNIAGFN
                     QVWEYVKHEFLDVKERRSFLGGFVGYKVRGYWPLLLRMWLGLMWAFEGINKIGEGWLN
                     FAKGSSSGWMFSPGVVQAGVKVAEATSAASEATGDWAAAAAPAAADAVAAASEAVAET
                     AVAAADGATKAFAKVWDLTKPIIDPNSGFVTWFRTTFMDGIFAHLPFQAMQVMVVFME
                     IGIGLALIGGFFTWWAAVASIAMVLLFTFTGMFAWDQAWFLFAAIVMMGGAGRAFGLD
                     NWSVPFFKKWWNGTKFARKWHFYADDPSK"
     gene            4019..4768
                     /locus_tag="PLG14_04565"
     CDS             4019..4768
                     /locus_tag="PLG14_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48438.1"
                     /translation="MPGLEIFGTAASLVIAASLMMRNLKRLRLINLAGSLMFALYGLG
                     IKSLPVFLVNVFIVLIDAWYLRRLRLEVSAFSLLRAAPGDSAYLAEFLRFYAKDIARH
                     VPGFSLEGPGGAAANGGTAEGAGAEGGAAGGTEAVFVLRDLVPASLVVYRKRGEGVYD
                     ILLDYAVPASRDYKSAEFFFDVASKEIAGGAEARFYTRSSVPVHERYLKRLGFVASDK
                     GGEEAGPKGDAKGARKGPGGQGREYARTVRP"
     gene            5062..>6710
                     /locus_tag="PLG14_04570"
     CDS             5062..>6710
                     /locus_tag="PLG14_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOX48439.1"
                     /translation="MDFQQFGIDERLISGAQALRSRALFHEKMLERAVAADENVCAKI
                     ALDEGREAVLLLPALQWLASAPVGEAPRRILCLAPDRASAERLAVAARVLGAGLDLRA
                     CVVTPPEEGEGAATGPASAPVLEGDPGAVLVAGLPESLLAAEAAGLLSLRSFGYLLVE
                     GAERVAELAPELLRRLSSAMLPPWERKSILSCSRLTVKAKSLAWDLADNPVEIEIEEQ
                     VAKAQSVASETWQVETGAKLRFVLGLLRRERPARLCLFCDLKSSAEEVALRLRENGIE
                     AAFVVGALPEERKFAVLERIKEVPGSVLVLSDEGAAGVTPGEFPLVLNYELPLEPDLY
                     VRRLEMLDRAAPGARVVNLACDRYAVGLPAIERYIDAKLEARPAGPELLEAEDASADL
                     VFEAARPERDSRGGGGSKVGRLRTADEAPRGQGGQGGRGRGEGRRDGRPEGRGGRDGG
                     GRGDNRRGDGRGGRGRDGYQRPDRSPDIRKSISDLTGGSLDVDGPGDLRGPEAASSGE
                     RGGQPRGERQGQRGGRGERGRQQGSGRGKGRGGKSQGRAPVS"
     assembly_gap    6711..6810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6811..7415
                     /locus_tag="PLG14_04575"
     CDS             <6811..7415
                     /locus_tag="PLG14_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48440.1"
                     /translation="SGRGKGRGGKSQGRAPVSPGPIQSAGRGAGNPYDLPMEERMKRY
                     REKYGQRGEEGALEGGRPEGGRPGRGQGNQGGARKGEGRRQAEVGQGGQAGKGQAQRN
                     GQRQAPKGEAQKGQKKQAAGEGQRGGRQDQSGRGGQQGRGQGQGQRGGQAQRGGQDQR
                     GRQPQRGGQAGRPASAPAAKPVEPKGFLGKLLGAFKKKSD"
     gene            7652..10198
                     /gene="glgP"
                     /locus_tag="PLG14_04580"
     CDS             7652..10198
                     /gene="glgP"
                     /locus_tag="PLG14_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOX48441.1"
                     /translation="MKIISHTVKPKIPAPLAPLEEIAHNLWLSWNFEAIMLFIRLDYD
                     VWLESAQNPAKMLGMVSQERYEELSQDDSYMAALKEVYAKFERYRKGETWYEGSREES
                     VAYFSMEYGMDVSLPTYSGGLGILSGDHMKTASDMGLPLVGVGLLYRQGYFKQYLNPD
                     GYQQESYPENDWYNMPVHRCLDKDGAAIKIAVDMAGRLVAAQIWEVKVGRSSLFLLDT
                     NIEENGPEERTITAALYGGDKESRIRQELLLGVGGIRALRALGVKVAATHMNEGHSAF
                     LALERIRGIMAERKFSFAEAAEAVIPTNIFTTHTPVPAGNERFGIDLMEKYFRPWAQE
                     LGLEWKDFLALGRERPEDQGEPFCMTVLALKLSAYSNGVSQLHGEVSRTMWKGLWPGL
                     PEAEVPIGAVTNGVHPRTWVSHNMLELLDRYFGPRFYDEPANLEMWDRMDRISDEELW
                     RTHERRRERLVVFARDRIKRAAKRVGAGEGALLRAEDALSPYALTISFARRFATYKRG
                     NLLLRDPERLLRLLSDRDRPVQIIFAGKAHPHDLPGKELIKELVHFSRREEVQSRIVF
                     LEDYDMTMARYLTSGSDVWLNTPRRPLEASGTSGMKAGMNGVLNCSVLDGWWAEAYAG
                     HGPTIGWAIGSGEMYADEELQDRVESEALYDLLEREIVPEFYARGRDGLPRAWIRMMK
                     ASMRCVGKEFSSHRMLKEYSERFYFPALENSRRLEVEGLAPAKAAAAYLEKARRAWHA
                     VSVSELRTDAKPVMERGDVVTVSALVELGGLEPSEAAVELYRGPLSNRDEIAEPSRIE
                     MEAVERRGSAWEYRVKVTCEDTGKQGYAVRVLPKHPALNGYFVPGLVRWA"
     gene            complement(10195..>10939)
                     /locus_tag="PLG14_04585"
     CDS             complement(10195..>10939)
                     /locus_tag="PLG14_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOX48442.1"
                     /translation="RANRARHWLRGGYPEAYGAAGDEAALAWLARYADSIAEERFAQA
                     GLPWAPGRTRSLLSMLARSHGSPLNESAVARSLGVSRPTVARAVAALGRSGLSRILPS
                     LRPASGKRVQGSGAFYLRDSGLLHALLGIAGTEGLLGSAALAASFEGYAIEEALALLP
                     EGVEAARYRTQDGAGLELVFLREGKPVAAAALRWAREGGPGKGALLAARDVGAPANWL
                     VLPESEERELPSGFTALGLARFLELVQEL"
BASE COUNT         1628 a         3780 c         3922 g         1509 t
ORIGIN      
        1 aacatggaag cgtcgttcac ttgctcgctt ggacgcgagg cgcggcgccg ccgcctgccc
       61 ggggctagac cgccttcacg aggtgctcgg ccaggatctt gatccctatc ccgatcagca
      121 ccacgccgcc cgcgacctcc gcccagcgct cgaacttcgc gccgatgcgc ttgccgaact
      181 cgcagccgaa gaggcagagg ccgaaggtga cgaggccgat gatcgcggcg ggcccgagga
      241 tgggcgtgcc gagcatgctg taggagaggc cgacggccag ggcgtcgagg ctcgtcgcga
      301 cggcgaggac gaggaggccc ccgaggtcga ggatgctgcg cttcgcggcc ttgggcttgg
      361 cggcctcggc gccgcccggc ccgaccgatg tggcggcgcc cggcccgacc gacccggccg
      421 cggcggccgc gggggccgga tcggcgcagg ccgactcctc ctcgagctcg aaggactcct
      481 tgagcatctt cccgccgacc agggcgagga gggcgaaggc gatccagtgg tcgaggccct
      541 ggatgtaggc gcggaatgcc gagcccgcga gccagcccgc gacgggcatg aggaactgga
      601 agaggccgaa ggcgaaggcg gcccggaggg cgtggcgcgc cttcatgtgg gggatgcaga
      661 tcccgctcga cacggagacg gcgaaggcgt ccatggaaag gcccagggcg acgagcaggt
      721 aggtgagcat ggttcctccg ggaggggcgc ggcgccggac gccgcgtcat cgtctcagta
      781 cttgcggctc aagagcttgt ccgcgacgcg cgcgagctcc tcgcgggctt cgcagcgggg
      841 caggcgggct atctcggcct gggcccgcgc ggtgaagcgg gccgcgtcgc gccgggccgc
      901 ctccacgccc ccgagctcgg aggcgcgggc caggatggcc gcgactgcct cgtcgcgggc
      961 gaccgcatcg ccgcgggcca aggcttcctc gcgggcgccg gcctcggctc ggtctgcccg
     1021 gccggggcgc ggcaggcggg agaggagggc gcgcatgccc gccccgtcgg cccgcagcgc
     1081 gtggatgagg gggagggtgc agaggccctc ggcgacgtct cggcccacgg gcttccgcat
     1141 cgtaccctcg ctggactcga agtcgaggat gtcgtcgatg acctggaagg ccatgccgat
     1201 gtcgtagccc gcgcgcctaa ggcgctcggc cacgggggga ggggccttgg actccgtcgc
     1261 gccgacgtgg agggagaggg agaagagggc cgcggtcttg ccggcgatct tccggaggta
     1321 ggagcggacg ctcggggagt agctcccctt gtccaggtcc tgcctgatct ccgcggcgca
     1381 gatggccgtg atgaggcggg caagggcctt ggcgttgcct gggcccgcgc tctccgccgc
     1441 gaggaggaag cagcgggcga agagccagtc ccccgcgagg acggcctcct tcgcgccgaa
     1501 gcgcgagtgg atggtcggca cgccccggcg gagctcggcc tcgtcgatca cgtcgtcgtg
     1561 gatgagggtg gagacgtgga ggagctcgac ggcggcggcg agggggagga tcctctcctc
     1621 gtaccctcgc ctgccgaagc cggcgccgat gaggatgagg gccggcctga gcatcttgcc
     1681 gttggacgcg acgatggagg ccacggcctc ctccaggggg aagtcggagc cgcggacgct
     1741 ctggtccatg agggcggcca ccttttccag ggaggccgct atctcggggg agtcctgcca
     1801 gaattcggtc attcgctgcg ctccgctcgg ggatggttgg ggagggtagc gccgcggaag
     1861 gaggcgggac cgccgccggg cgccttggcg cgcccgacgg cggattcccc ctcccgcggc
     1921 cttaggcggg gccgccggtc ctacttggag ggatcgtcgg cgtagaagtg ccacttcctc
     1981 gcgaacttgg tgccgttcca ccacttcttg aagaagggca cggaccagtt gtccaggccg
     2041 aaggcgcgcc cggcgccgcc catcatgacg atggcggcga agaggaacca ggcctggtcc
     2101 caggcgaaca tgcccgtgaa ggtgaagagg aggaccatcg cgatgctcgc gacggcggcc
     2161 caccaggtga agaagcctcc gatgagggcg aggccgatgc cgatctccat gaagacgacc
     2221 atcacctgca tggcctggaa ggggaggtgg gcgaagatgc cgtccatgaa ggtcgtgcgg
     2281 aaccaggtga cgaagccgga gttcgggtcg atgatgggct tcgtgaggtc ccagaccttg
     2341 gcgaaggcct tggtcgcgcc gtcggccgct gcgaccgcgg tctcggcgac ggcctcgctg
     2401 gcggcggcga cggcgtcggc cgcggcgggg gcggcggcgg cggcccagtc gcccgtggcc
     2461 tcggaggcgg cgctcgtcgc ctcggccacc ttcacgcccg cctgcacgac gccgggggag
     2521 aacatccagc cggagctgga gcccttggcg aagttgagcc agccctcgcc gatcttgttg
     2581 atgccctcga aggcccacat gaggccgagc cacatgcgga ggaggagggg ccaatagccg
     2641 cggaccttgt acccgacgaa gccgccgagg aaggaccggc gctccttcac gtcgaggaat
     2701 tcgtgcttga cgtactccca gacctggttg aagccggcga tgttgaagag gtagtagacg
     2761 ttgatgaagt gcttgatgaa gagggcgaac cagcccgtcg tcttcatgtt gcccgcgttg
     2821 gagacgccga catggctgcc gatgcagacc atgacgccgt ggtagttggg cgtgaacttg
     2881 tggcgctcgc cgccgtcgat gtcggcgatg atgttcttcg cggccgcctc ggcggtgaag
     2941 tgggcggtct cgacgatctg cgcgaggggc ttgccgtcgc ggatgaggcc ggcgttgtcg
     3001 ccgacgacgt acacgaaggg gtacttcgtg gacttcatct cctcgtcgca ctcgatgcgg
     3061 ttgcgcttgc ccatcgggac ctcgagggag ccggcgaagg aggagccctt cacgccgcag
     3121 gtccagatga aggtgtcggt ctccaggact ccgccgtcct tgaggaggac cttccccggc
     3181 tcggcgccga tgatggcgct gcccagcatg atctcgcagc cggccttctt gaggtagccc
     3241 tcggccttgg cgcggagggg ctcctcgagg atggggagga tggagggcag tgcctccacg
     3301 cagacgaccc tcgtctcctt cgggtcgagg aagtgcttcc tgcacatcgt gtcgcggtac
     3361 tcgaggagct cgccgatcat ctcgatgccg gtgaagcccg cgcccgcgac cacgaaggtg
     3421 aggagcctgc ggcgcttcgc ggcgtcgggc tcggcggccg ccttggcgaa ggtctcctcc
     3481 aggtggtggc ggatccgcat cgcgtcctcg aaggaccaga gggagaagga gttctcctgg
     3541 acgccgggga tgccgaagta ctcgggctcg gcgccggcgc ccacgacgat gtagtcgaag
     3601 gggtactcgc ggagggcgga gaaggccttc ttggcctcga agtcgacctt gaggatcttg
     3661 tcggtggcga cctcgatgcg cttggcgccg aagatcttcc ggaagctcac ctggaccgac
     3721 tcgggctcgg tgcgccagcc ggcgacctcg tggagctcgg tcatgagggt gtggtagggc
     3781 ttcatgtcga cgagggtgat ctccacgtcg tcgcgcttgc ggaagcgctt ctccaggatc
     3841 ttgcccgccc agacgccgcc gtagccgccg ccgaggatga gtattcgctt cttgtctccc
     3901 attcggatca ctccttgtcg ggtgtggtat gctgttgaga ttttagacaa tcgataatgg
     3961 acgatatagg ttgaaaagtc aataaagcgt tctataattt cgtatccaag gagggtccat
     4021 gccaggccta gagatcttcg gcacggccgc gagcctcgtg atcgccgcct ccctcatgat
     4081 gcggaacctg aaacgcctaa ggctcatcaa cctcgcgggc tctctcatgt tcgcgcttta
     4141 cggcctgggc atcaagtccc tgcccgtctt tctcgtcaat gtcttcatcg tcctgatcga
     4201 cgcctggtac ctgcgccgcc tcaggctcga ggtctccgcc ttctccctcc tccgcgcggc
     4261 tcccggcgac tccgcctacc tcgcggagtt cctgcgcttc tacgcgaagg acatcgccag
     4321 gcacgtcccc ggcttctccc tcgaaggccc gggcggagcg gcggcgaatg gcgggacggc
     4381 ggagggtgcc ggcgcggagg gcggcgccgc gggcgggacg gaggcggtct tcgtcctgcg
     4441 cgacctcgtg cccgcgtcgc tcgtcgtcta ccgcaagcgc ggcgagggcg tctacgacat
     4501 cctcctcgac tacgccgtgc ccgcctcccg cgactacaag agcgcggagt tcttcttcga
     4561 cgtggcctcc aaggagatcg cgggcggggc ggaggcccgg ttctacacga ggagctcggt
     4621 gcccgtgcac gagcgctacc tgaagcgcct cggcttcgtc gcctcggaca agggcgggga
     4681 ggaggccggg ccgaagggcg acgcgaaggg cgcgcggaag gggcctgggg gccaaggccg
     4741 ggagtacgcg cggaccgtgc gtccctgagc ttccaatttc cccgccggca tagtactatc
     4801 cggaattacc gggttttccg cgcgctttcg ggcatggctc gagagcgccg gggcgcgggg
     4861 cgagccgcct tgaggccgcc tccccccatt ttttcggcgc cgcggcgccg ggaagaccgc
     4921 ggacggcgcc atgggcgctt tccgccccgg gcacgcggtt ccgcgcgcct cccgcgcggc
     4981 ccgaacggcc cgactcgagc ccggctcgcc gggggcccta ggctaggccc cccatccttg
     5041 actccagcaa gcgagcgtag catggatttc cagcaattcg gcatcgacga acgactcatc
     5101 tccggcgccc aggcgctcag gagccgcgcc ctcttccacg agaagatgct cgagcgcgcc
     5161 gtggccgcgg acgagaatgt ctgcgccaag atcgccctgg atgagggccg cgaggccgtc
     5221 ctcctccttc ccgccctcca gtggctcgcc tccgcccccg tgggcgaggc cccccgccgg
     5281 atcctctgcc tggcgcccga ccgggcctcc gccgagcgcc tggccgtcgc cgcccgggtc
     5341 ctgggcgcgg gcctcgacct tcgcgcctgc gtcgtcactc cccccgagga gggcgagggc
     5401 gccgcgaccg gaccggcctc cgcccccgtc ctggagggcg atcccggcgc cgtcctggtc
     5461 gccggccttc ccgagtccct cctggccgcc gaggccgccg gcctcctctc cttgcgctcc
     5521 ttcggctacc tcctcgtcga aggcgccgag cgcgtcgccg agctcgcccc cgagctcctc
     5581 cgccgcctct cctccgcaat gctacccccc tgggagagga agagcatcct ctcctgctcc
     5641 aggctgaccg tgaaggcgaa gagcctggcc tgggacctcg cggacaatcc cgtggagatc
     5701 gagatcgagg agcaggtcgc gaaggcccag agcgtcgcga gcgagacctg gcaggtcgag
     5761 acgggcgcga agctccgctt cgtcctgggc ctcctgcgcc gcgagcggcc cgcgaggctc
     5821 tgcctcttct gcgatctcaa gtcgagcgcc gaggaggtcg cgctgcggct ccgcgagaac
     5881 gggatcgagg cggccttcgt cgtcggcgcc ctgcccgagg agcggaagtt cgccgtcctg
     5941 gagcggatca aggaagtccc cggctccgtc ctcgtcctct ccgacgaggg ggccgcgggc
     6001 gtgacccccg gcgagttccc cctcgtcctc aactacgagc tgcccctcga gcccgacctc
     6061 tacgtgcggc gcctcgagat gctcgaccgc gcggcccccg gggcccgcgt cgtcaacctg
     6121 gcctgcgacc gctacgccgt cggcctcccc gccatcgagc gctacatcga cgcgaagctc
     6181 gaggcgcggc cggccggccc cgagctcctc gaggccgagg acgcgagcgc cgacctggtc
     6241 ttcgaggccg cgcgccccga gcgggactcg cgcggcggcg gcggctccaa ggtcggccgc
     6301 ctgaggacgg ccgacgaggc gccgcgcggc cagggcggcc agggcggacg aggccgcggc
     6361 gaggggcgcc gcgacggcag gcccgagggc cgcggcgggc gcgacggcgg cgggcgcggc
     6421 gacaacaggc gcggcgatgg ccgaggcgga aggggccggg acggctacca gcgccccgac
     6481 cggagccccg acatccgcaa gagcatctcc gacctcacgg gcggctccct ggacgtcgat
     6541 ggcccgggcg acctccgcgg gccggaagcg gcgtcttcgg gcgagcgcgg cggccagccc
     6601 aggggcgaga ggcagggcca gcgcggcggc cgcggcgagc ggggccgcca gcagggctcc
     6661 ggccggggca agggcagggg cggcaagtcc cagggcaggg cgcccgtctc nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctccggccg gggcaagggc aggggcggca
     6841 agtcccaggg cagggcgccc gtctctccgg ggccgatcca gagcgcgggc aggggcgccg
     6901 gcaatcccta cgaccttccg atggaagagc gcatgaagcg ctaccgcgag aagtacggcc
     6961 agcgcggcga ggaaggagct cttgagggcg gacggccaga aggcggaagg cccgggcgcg
     7021 gccagggcaa ccagggcgga gcccggaagg gcgagggccg ccgccaggcc gaagtcggcc
     7081 agggcggcca ggccggcaag ggccaggccc agcggaacgg ccagaggcag gctcccaagg
     7141 gcgaggccca gaagggccag aagaagcagg cagccggaga gggccagcgg ggcggccggc
     7201 aggaccagtc cggacgcggg ggccagcagg gccgcggcca gggccagggc cagcgcggcg
     7261 gccaggcgca gaggggaggc caggatcagc gcggaaggca gcctcagcgc ggcggccagg
     7321 ccggtcgtcc cgcctccgcc cccgcggcca agcccgtcga gcccaagggc ttcctcggca
     7381 agctcctcgg cgccttcaag aagaagagcg actaggattc gcctgcgcgc cacgccggtt
     7441 tgcgggcctc gcccgggccg gcttggcctt gccggccgac cccgtcatgg gggccggttc
     7501 ggcgctgtaa accggtttac acttgacgga acctccccgc tccactaggc ttttgccgat
     7561 gcgtccctga ttcccacggc ggaggatcca ccgccggccg tcggccggct tcctcctccc
     7621 ctccccgcaa cgaccacgaa ggcggcccgc gatgaagatc atctcccaca ccgtaaagcc
     7681 gaagataccc gcccccctcg cccccctcga ggagatcgcg cacaacctct ggctctcctg
     7741 gaacttcgag gcgatcatgc tcttcatccg cctcgactac gacgtatggc tcgagtcggc
     7801 gcagaatccc gccaagatgc tcgggatggt ctcgcaggag cgctacgagg agctctccca
     7861 ggacgactcc tacatggcgg ccctcaagga ggtctacgcc aagttcgagc gctaccggaa
     7921 gggcgagacc tggtacgagg ggagccgcga ggagagcgtc gcctacttct ccatggagta
     7981 cggcatggac gtctccctgc ccacctactc gggcggcctg ggcatcctct cgggcgacca
     8041 catgaagacg gcgagcgaca tgggcctgcc cctggtcggc gtcggactcc tctaccgcca
     8101 gggctacttc aagcaatacc tcaatcccga cggctaccag caggagtcct accccgagaa
     8161 cgactggtac aacatgcccg tccaccgctg cctggacaag gacggagcgg cgatcaagat
     8221 cgccgtcgac atggccggcc gcctcgtggc ggcgcagatc tgggaagtca aggtcggccg
     8281 ctcctccctc ttcctcctgg acacgaacat cgaggagaac gggcccgagg agcggacgat
     8341 cacggccgcc ctctacggcg gcgacaagga gtcgaggatc aggcaggagc tcctcctggg
     8401 cgtgggcggc atccgcgccc tccgggccct cggcgtcaag gtggccgcga cccacatgaa
     8461 cgaggggcac tccgccttcc tcgccctgga gcggatccgc gggatcatgg cggagcgcaa
     8521 gttcagcttc gcggaggcgg ccgaggccgt catcccgacc aacatcttca cgacccacac
     8581 gcccgtgccc gcgggcaacg agcgcttcgg catcgacctc atggagaagt acttccgccc
     8641 ctgggcccag gagctcggcc tcgagtggaa ggacttcctg gccctgggcc gcgagcggcc
     8701 cgaggaccag ggcgagccct tctgcatgac cgtcctcgcg ctcaagctct ccgcctactc
     8761 gaacggcgtc tcgcagctcc acggggaggt ctcgcggacg atgtggaagg gcctctggcc
     8821 cggccttccc gaggccgagg tgcccatcgg cgccgtcacg aacggcgtcc acccccggac
     8881 ctgggtatcg cacaacatgc tcgagctcct ggaccgctac ttcgggccgc gcttctacga
     8941 cgagcccgcg aacctggaga tgtgggatcg catggaccgg atctccgacg aggagctctg
     9001 gaggacccac gagcgccgcc gcgagcgcct cgtcgtcttc gcgcgcgacc gcatcaagcg
     9061 ggccgcgaag cgcgtcggcg ccggcgaggg cgccctcctc cgcgcggagg acgcgctctc
     9121 gccttacgcc ctcacgatct ccttcgccag gcgcttcgcc acctacaagc gcggcaacct
     9181 cctcctccgc gaccccgagc gcctcctcag gctcctctcc gaccgcgacc ggcccgtgca
     9241 gatcatcttc gccggcaagg cccatcccca cgacctcccg ggcaaggagc tcatcaagga
     9301 gctcgtccat ttctcccgca gggaggaggt gcagagccgc atcgtcttcc tggaagacta
     9361 cgacatgacg atggcgcgct acctgacctc gggctcggac gtctggctca acacgccgcg
     9421 ccgccccctg gaggcctcgg gcacgagcgg gatgaaggcg ggcatgaacg gcgtcctcaa
     9481 ctgctcggtc ctggacggct ggtgggcgga ggcctacgcg ggccacgggc cgaccatcgg
     9541 ctgggccatc ggctccggcg agatgtacgc cgacgaggag ctccaggacc gcgtggagag
     9601 cgaggccctc tacgacctcc tggagcgcga gatcgtcccc gagttctacg cgcgcggccg
     9661 cgacggcctg ccgcgggcct ggatcaggat gatgaaggcc tcgatgcgct gcgtcggcaa
     9721 ggagttctcc tcccaccgca tgctcaagga gtactcggag cgcttctact tccccgccct
     9781 ggagaattcg cggcgcctcg aggtcgaggg gctggccccg gccaaggccg ccgccgccta
     9841 cctggagaag gcgagaaggg cctggcacgc ggtctccgtc tcggagctcc gcaccgacgc
     9901 gaagcccgtg atggagcgcg gcgacgtcgt cacggtgagc gccctcgtcg agctcggcgg
     9961 cctcgagccc tcggaggcgg ctgtcgaact ctatcgcggg cccctctcga accgggacga
    10021 gatagccgag ccctcgcgga tcgagatgga ggccgtggag cgccgcggct ccgcctggga
    10081 gtaccgcgtg aaggtgactt gcgaggacac ggggaagcaa ggctacgcgg tccgcgtcct
    10141 gccgaagcac cccgccctca acggatactt cgtgccgggc ctggtgcgct gggcctagag
    10201 ctcctggacg agctcgagga agcgggcaag gcccagggcc gtgaagcccg aggggagctc
    10261 cctctcctcc gactcgggaa ggacgagcca gttcgcggga gcgccgacgt cgcgcgccgc
    10321 gaggagggcg cccttcccgg gaccgccctc gcgggcccag cggagggccg ccgcggcgac
    10381 gggcttcccc tccctgagaa agacgagctc gaggccggcc ccgtcctggg tccggtagcg
    10441 cgccgcctcc accccctcgg gtaggagggc cagggcctcc tctatggcgt agccctcgaa
    10501 ggaggcggcg agcgccgcgc tgccgaggag tccctccgtc cccgcgatgc cgaggagggc
    10561 gtggaggagg ccggagtccc tgaggtagaa ggctcccgag ccctggaccc gcttgcccga
    10621 ggccgggcgg agggagggca ggatccgcga gaggccgctc cgccccaggg cggccacggc
    10681 ccgcgcgacg gtgggcctcg agacgcccag ggagcgggcg accgcgctct cgttcagggg
    10741 ggagccgtgg gagcgcgcca gcatggagag gaggctcctc gtgcggccgg gcgcccaggg
    10801 caggcccgcc tgggcgaagc gctcctcggc gatcgagtcc gcgtagcgcg cgagccaggc
    10861 cagggccgcc tcgtcgcccg cggcgccgta ggcctcggga tagccgcccc tgagccagtg
    10921 ccgcgcgcgg ttcgcccgg
//
LOCUS       DAOOSJ010000062        10904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_3951_length_10904_cov_5.586229, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000062 DAOOSJ010000000
VERSION     DAOOSJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3951_length_10904_cov_5.586229"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLG14_04590"
     CDS             <1..323
                     /locus_tag="PLG14_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOX48443.1"
                     /translation="DDVMVITSQGKTLKIAADTVNIQGKGARGVRIVNIDKPDFVIGL
                     DKLIREEEEVLASEAEAEVGSEGDGAEAAALKAASEEGLPVEQADEAESPAEPKDEDT
                     DSEN"
     gene            complement(416..769)
                     /locus_tag="PLG14_04595"
     CDS             complement(416..769)
                     /locus_tag="PLG14_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_250697390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX48444.1"
                     /translation="MPRTLKSWSYLANGTTLRLEEERCVGCGLCLEVCPHGVFSLRQA
                     AGTSTIASAEPAGSAKAAIVARESCMECGACAWNCPCGALSVRTGVGCAVAVLNGRKR
                     GGSPDCCCDEESGCC"
     gene            complement(780..1853)
                     /gene="hgcA"
                     /locus_tag="PLG14_04600"
     CDS             complement(780..1853)
                     /gene="hgcA"
                     /locus_tag="PLG14_04600"
                     /inference="COORDINATES: protein motif:HMM:NF040863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HOX48445.1"
                     /translation="MLLKALPATAELQARLSQPFVEGSFATAQGAVPRISTRLTLKDR
                     LGALRVRLSIGRSSYRILPGLYAAGSPSRSSPVFVTANYKLSFDALKQALAGIHSWIL
                     VIDTRGVNVWCAAGKGSFGNEELARRIASTRLALHVDHRELILPQLAGPGVSAPRFAK
                     DHGWRLRFGPVRAADIPAYLAAGLAKTEEMRRVRFSARDRLVLAPLELVAGLKKLAPC
                     LLLFLTAAFLAAPKDPGAALARSAWAFLPFALAYAATTLLFPLLLPALPGRAFSLKGA
                     ILGLTLASLAILASKAPPLLALSYLLAMPALSGWFGLLFTGSTTYTSLSGVELEVRAA
                     RLPLLIAGAAGLGLRLALLAGRI"
     gene            complement(1844..2320)
                     /locus_tag="PLG14_04605"
     CDS             complement(1844..2320)
                     /locus_tag="PLG14_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX48446.1"
                     /translation="MQVIVDSTSGEESLRALRRDLRRIERALERGLRGRTQAGCCGIG
                     KAQCHALLALRSGGKPLSALAKELSVEASTLTRTLDGLEGAGLVRRHGSGEDRRRLLV
                     EASAAGREKIAEVDGAWNARLAEALELIPADKRGLVEEGVALLAAALAHGQEEPCC"
     gene            complement(2403..3689)
                     /locus_tag="PLG14_04610"
     CDS             complement(2403..3689)
                     /locus_tag="PLG14_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX48447.1"
                     /translation="MPDNFTLSLYKVAPYKGCAHGCRYCDGRAEKYYVEGDFERDVEV
                     RDAVPARFAAELPSVRERGMVAFGSGVTDPYQPLEAGLRLTGACAEALAAACGERSAP
                     AESGRPAPTAAAPCATILSKAAAQTGRGQGGGESAELFESGPSARPLPALVMTKSALA
                     LRDLPAWRRVNKAAGFVLLVSITSLDEELREAMEPGASSFAERLALLKAFKEAGCAVG
                     ALAMPFLPGLSDDVASVERLYEALAAVPVDFVMPGGLTLRPGRQKDFYLKALEAYRPG
                     LLPSTKAIFAEERASGSPTAAARKELFARLAPIRNKFGLPYLLPHRVFARLLPPHDAF
                     RVLLRDMAELYGERRVDTGALVKSAEAYDAWLLGLRREFRRRRSLPKDWLEERFAAAL
                     ADGSLYRLLGNPRLASFSASVLEEGARLDFTTLKLE"
     gene            complement(3701..5947)
                     /locus_tag="PLG14_04615"
     CDS             complement(3701..5947)
                     /locus_tag="PLG14_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48448.1"
                     /translation="MSSKRRPLLALLLAGLAAAPLAALENLDRAALEKGAALAEVSFL
                     AAPYDAERPFPDPADPGFAPYAEGPLAASPEGRRSYCLKASFLLEEGAGPVSLILPPS
                     DYPFALSLNGHLLLEVGLHRERYVSNIFLSTALRLPEALLSPPGGPNELLVAFYPLQG
                     DEDYPLEFPRLATPLKATGSVFLRNFLRVEMVTASSFAALILALYFFFLALSGRERLS
                     YAFFALLCLSFAAAYLEISLQTDALPELLLKKISKTGFLGMLFFFPAFVAAYTGRPRA
                     RKPILLAWGGIGLALSLAFLFAKDKLGVDRVFGPAMTFEFILALGGSIALLAISAFKD
                     RRQGSAILLGGALLSVAASGTDIVAISGGKLPYLYFTPYGFLGVVVVIFVVLAKEQVS
                     VAEESRRQAALLKERSAEEGRTMEKVRDLSASLVEAERELKAATDEAERLIAESAAAT
                     RRAAAESSERAGEAGRVAALLAEQIERSASRIPEAVRGQTEFAESVTSTLSAMNQSIE
                     ALSKEALGGAAASEGLAKRGDEAARVVADSKSAAASISGAAAYLREVIGQVGDLADKT
                     NTLAINTAIEAARAGQAGRGFAVIATAIRALAAESSERLAATASRLAELEEAVRKNES
                     ASTRVEAGLSGIIGSGRETARVIGRISEGILERREESKRLVEEARRLLEDTRAVEELF
                     GRALAENAEARNRVSGLAESFASMERLLVAEAERGGRLEAAVARVLGVAARDREIVAG
                     LESIVRGS"
     gene            complement(6080..6547)
                     /locus_tag="PLG14_04620"
     CDS             complement(6080..6547)
                     /locus_tag="PLG14_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48449.1"
                     /translation="MGILGKIGIGHAEFLAEDWLPREEREYAAYRAFDEARRRARLRR
                     LASWAARIGRALGRIGGPGFPGGPDNDARPGFDEAPPETSRFVGTWDEERGYRRGLPP
                     LSRRRKAEFVRRLLSCPPDTLPSPPFRRGSGGLYLVGGEASLLALELRRSLGF"
     gene            complement(6606..7616)
                     /locus_tag="PLG14_04625"
     CDS             complement(6606..7616)
                     /locus_tag="PLG14_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX48450.1"
                     /translation="MKPKLASIADLAKLAGVSKSTVSRALSGSPLVGAETRERIEALA
                     REHGFRPNLPARYLSLGSSRSVAFVTSDCSPCGAGSGSADLFNLEIMGGVAQGLRELG
                     YDMLALYVEPGDEGWIADYLDSGLVAGFILMTYEQKSRRLELLAEREAPFIAWGIGEG
                     RYCSVTGDNASGGRLAADRLLGRGARRIAFLGGPEDDLESGERRRGFSELLSEAGLPL
                     ESERLVFGDYSEESGALAMERLLKADPKLDAVFAASDLMAIGAMRVLKARGLRIPEDV
                     AVVGYDDLRLAAYVSPSLTTISQHISLTGKLLARDLVAFLGSGVVTNRVVPVELVARD
                     SA"
     gene            7786..9666
                     /locus_tag="PLG14_04630"
     CDS             7786..9666
                     /locus_tag="PLG14_04630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX48451.1"
                     /translation="MRSRRSWAAGKRPSSRLSLAAALCSSLLLLDCREAPAREAPAFR
                     QQPPSLSEAERAYLERLGPVPLCVDSDWEPYEYLDEKGEYAGIAADLVRLIAARSGVE
                     FRLLRTRDWAESLEASKTGACLALAFLNASPEREGWLVFTEPYFSDPNVFITRNGHDY
                     IADPARLSGERIALPAGTSVEELVRARYPNLEVVTVDSEREALRLVDSGEADMTLRSL
                     TMAAYVIRKEGLFNLRISGQLPGLVNEFRMGVAKGEPLLKDILDRGVRSLSPQEVQAA
                     VNAHIAIEARTAADPRPLAWATGAFAVLSLLWLLWSLAQRNYLKHLRFVLDAMPAYVF
                     VKDARGRYLLANRWMASAFGVPPERVKGLTDADCGGSAEEMEAWRAEDRQVLESGKPL
                     RIDNHPGRRVDGSPGWFQTIKMPYRRPDTGKKAILGVTLDVTDLKNAELELERREQRF
                     RHLAQHDGLTDLPNRGLFSDRLGQAMALCRREGTRLALVFLDLDHFKEVNDIHGHRAG
                     DLLLTEAARRLKAAIRASDSAGRIGGDEFVLFLLDIEDRARAAAAAEKARAAMELPFL
                     VEGEELRVSASIGIALFPDDGEDETALSRRADAAMYRSKALGRNRVSVYDPGLDSGVE
                     AK"
     gene            complement(9701..10039)
                     /locus_tag="PLG14_04635"
     CDS             complement(9701..10039)
                     /locus_tag="PLG14_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48452.1"
                     /translation="MSRVSAANAQAVSAFLQALRAAPPKLAQSRLLNTADRDLAVLLS
                     AASEEDLREVLAFVGAAKEQRVREELVRMAHVRLPPETVARLAEHLSAHVSGDRPLGP
                     ASRYFRPHSG"
     gene            complement(10336..>10904)
                     /locus_tag="PLG14_04640"
     CDS             complement(10336..>10904)
                     /locus_tag="PLG14_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOX48453.1"
                     /translation="PYEALRDTVIEEIIRPILEPTGLDMKDTKYFVNPTGRFVIGGPM
                     GDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMARYVAKNVVAAGLAERC
                     ELELAYAIGVPFPVSVMVETFGTSTVPEERIAAAVKKVFDLSPGGIIRELDLKKPIYL
                     RTAAYGHFGRDHFSWERTDRVEDLKKAL"
BASE COUNT         1670 a         3530 c         4211 g         1493 t
ORIGIN      
        1 acgacgacgt gatggtgatc acctcccagg gcaagaccct caagatcgcc gccgatacgg
       61 tcaacatcca gggcaagggc gcccgcggcg tcaggatcgt caacatcgac aagcccgact
      121 tcgtgatcgg cctcgacaag ctgatccgcg aggaggaaga agtcctggcg agcgaggccg
      181 aggccgaggt cggcagcgag ggcgacggcg ccgaggcggc cgccctgaag gccgcctcgg
      241 aggaaggcct ccccgtggag caggccgacg aggccgagag ccccgccgag cccaaggatg
      301 aggataccga ttccgaaaac tgagattgtt ccacgtgaaa caataggccg cccatccggg
      361 cggcctttct tttttccacg ccttctcttc gtgtgaatgt ttcacgtgaa acactctagc
      421 aacagccgct ttcctcgtcg cagcagcaat cggggcttcc gcctcgcttc cggccgttga
      481 ggacggccac ggcgcagccc acgccagtcc tgacgctcaa ggcgccgcac gggcagttcc
      541 acgcgcaggc cccgcattcc atgcaagact cgcgggcgac gatggcggcc ttggctgagc
      601 cggcgggctc cgcggaggct atagtcgaag tccccgcggc ctgccttagg ctgaagacgc
      661 cgtgggggca gacctcgagg cagaggccgc agccgacgca gcgctcctcc tcgagcctga
      721 gggtggttcc gttggcgaga tagctccagc ttttcagggt tcgtggcatc tttcttcctt
      781 tatatacgtc cggccaggag agcgagcctc agtcccaggc cggcggcccc cgcgatcagg
      841 aggggcagcc tcgccgcccg tacctcgagt tccacgcccg agagggaggt gtaggtcgtc
      901 gaaccggtga agaggaggcc gaaccagcct gagagggcgg gcatggcgag gagataagag
      961 agggcgagaa ggggcggcgc ttttgaggcg aggatcgcca ggctggcgag cgtcaggccc
     1021 aggatggcgc ctttgaggct gaaggccctg cccgggaggg cagggagtag aagggggaac
     1081 aggagggtgg tggctgcata ggcgagggcg aagggaagga aggcccaggc gctcctcgcc
     1141 agggcggcgc ctggatcttt cggggccgcg aggaaggcgg ccgtcaggaa gaggaggagg
     1201 cagggcgcga gcttcttgag gccggctaca agctcgaggg gcgcgaggac gaggcggtcg
     1261 cgggcagaga agcgcaccct ccgcatctct tctgtctttg cgagaccggc cgcgagatag
     1321 gccggaatat cagcggctct caccgggccg aagcggaggc gccagccgtg gtccttcgcg
     1381 aagcgaggtg ccgagacgcc ggggccggcg agctggggaa ggatgagctc acggtgatcg
     1441 acatggaggg cgagcctggt ggaggcgatc cgtcgtgcca gctcctcgtt gccgaagctt
     1501 cccttgcctg cggcgcacca gacgttgacg cctctagtgt cgatgacgag aatccaggaa
     1561 tgaatgccag caagcgcttg ctttaacgca tcgaaactga gtttatagtt ggccgtcacg
     1621 aaaaccggcg aactccgcga gggcgagccc gcggcataga ggccggggag aatccggtag
     1681 gaggagcggc cgatgctgag gcggacgcgc agggctccga gcctgtcctt cagggtcaga
     1741 cgagtcgaga ttctcggcac ggctccctgg gccgtcgcga agcttccctc gacgaagggc
     1801 tgggagaggc gggcctggag ttcggccgtg gcgggaaggg ccttcaacag cacggctcct
     1861 cctgtccgtg ggcgagggcg gccgccagga gggcgacgcc ctcctccacg aggccgcgct
     1921 tgtccgcggg gatcagctcc agggcctccg ccagcctggc gttccaggcg ccgtccacct
     1981 cagcgatctt ctcccggccc gccgcgctcg cctccacgag gagccgccgc cgatcctccc
     2041 ccgagccgtg gcgcctgacg aggcccgcgc cctcgaggcc gtcgagggtc ctcgtgaggg
     2101 tgctcgcctc cacggagagt tccttcgcga gggccgaaag gggctttcca cctgagcgga
     2161 gggcgaggag ggcgtggcac tgggccttgc cgatgccgca gcagccggcc tgggtcctgc
     2221 ccctgagccc gcgctccagg gcccgctcga tgcgcctgag gtcgcgccgc agggcgcgga
     2281 ggctctcctc gcccgacgtg gagtcgacga taacttgcat gctacaagta ttgcgcaatg
     2341 caaaggcctg ccgcaagccc ccatgcgctt cccgcgagcc cctcgcgcct tccggccgag
     2401 ccttattcga gcttgagggt cgtgaagtcg agccgagccc cctcctcgag gaccgaggcc
     2461 gagaaggagg cgaggcgcgg attgccaagg aggcggtaga gggagccgtc ggccagggcg
     2521 gcggcgaagc gctcctccag ccagtccttg gggaggctcc ggcggcggcg gaactcgcgg
     2581 cggaggccaa ggagccaggc gtcgtaggcc tccgcgcttt tcacgagggc gcccgtgtcc
     2641 acgcgtcgct cgccgtagag ctccgccatg tcgcggagga ggacgcggaa ggcgtcgtgg
     2701 ggcggaagga gcctcgcgaa gacccggtgc ggaaggaggt agggcaggcc gaacttgttc
     2761 cggatgggcg cgagccgcgc gaaaagctcc ttccgcgccg cggccgtcgg ggagcccgag
     2821 gcccgctcct ccgcgaagat ggctttcgtg gatgggagga ggccgggcct gtaggcctcg
     2881 agggccttca agtagaagtc cttctgcctg cccggcctca aggtgaggcc gccgggcatg
     2941 acgaaatcca cggggacggc ggcgagggcc tcgtagagcc gctcgacgga ggcgacgtcg
     3001 tcggagaggc cagggaggaa gggcatggcg agggcgccga cggcgcagcc cgcctccttg
     3061 aaggccttga ggagggcgag gcgctcggcg aagctcgagg cgcccggctc catggcctcg
     3121 cggagctcct cgtccaggct cgtgatcgac acgaggagga cgaagcccgc ggccttgttg
     3181 acccgccgcc aggcggggag gtcgcggagg gcgagggccg acttggtcat cacgagggcg
     3241 gggaggggcc gggccgaggg accggactcg aagagctcgg cgctttcacc tccgccctgg
     3301 ccgcggccgg tttgcgcggc ggccttcgaa aggatcgtcg cgcagggcgc cgcggcggtc
     3361 ggcgcgggcc gcccgctctc ggctggcgcc gacctctctc cgcaggcggc ggccagggcc
     3421 tcggcgcagg ctcccgtgag gcggagtccc gcctccaggg gctggtaggg atctgtgacg
     3481 ccggagccga aggccaccat gccccgctcg cggacggatg ggagctcggc ggcgaagcgg
     3541 gcggggaccg cgtcgcggac ctcgacgtcg cgctcgaagt cgccttcgac gtagtacttc
     3601 tccgcccggc cgtcgcagta gcggcagccg tgggcgcagc ccttgtaggg ggcgaccttg
     3661 tagagggaga gggtgaagtt gtccggcagg gggccgggcc tcaggagcct cgtacgatgc
     3721 tctcgagccc cgcgacgatc tcgcggtcgc gggccgcgac gcccaggacg cgggctacgg
     3781 ccgcctccag gcggccgccc cgctcggcct ccgcgacgag gaggcgctcc atcgaggcga
     3841 aggactcggc caggccggag acgcggttgc gcgcctccgc gttttccgcg agggcgcggc
     3901 cgaagagctc ctccacggcg cgcgtgtcct cgaggaggcg gcgcgcctcc tccacgaggc
     3961 gcttggactc ctcgcgccgc tcgaggatgc cctccgagat gcggccgatg accctcgcgg
     4021 tctcgcggcc ggagccgatg atgccggaga ggcccgcctc cacccgcgtg gaggccgact
     4081 cgttcttgcg cacggcctcc tcgagctcgg cgaggcggga ggccgtcgcg gccaggcgtt
     4141 cgctcgactc cgcggccagg gcgcggatgg ccgtcgcgat gacggcgaag ccgcggccgg
     4201 cctggcccgc gcgggcggcc tcgatcgcgg tgttgatcgc gagggtgttg gtcttgtccg
     4261 cgaggtcgcc gacctggccg atgacctcgc ggagataggc cgcggcgccg gagatggagg
     4321 cggcggcgct cttggagtcc gcgacgaccc tggccgcctc gtcgccccgc ttggcgaggc
     4381 cctccgaggc ggcggctccg cccagggcct ccttggacag ggcctcgatg ctctggttca
     4441 tcgcggagag ggtggaggtg acgctctccg cgaactcggt ctggccgcgc acggcctccg
     4501 ggatgcgcga ggcggagcgc tcgatctgct cggcgaggag ggcggcgacc ctgcccgcct
     4561 cgccggcccg ctcgctcgac tccgcggccg cgcgccgcgt cgcggcggcg ctctccgcga
     4621 tgaggcgctc cgcctcgtcg gtcgccgcct tgagctcgcg ctcggcctcc acgaggctcg
     4681 cggagaggtc gcggaccttc tccatcgtcc ggccctcctc agcgctgcgc tccttgagga
     4741 gggcggcctg gcgccgcgac tcctcggcga cggagacctg ctccttcgcg aggacgacga
     4801 agatgacgac gacgacgccc aggaagccgt agggcgtgaa gtagaggtag gggagcttgc
     4861 cgccggagat cgcgacgatg tccgtgccgc tcgccgcgac ggagaggagg gcgccgccca
     4921 ggaggatggc cgagccctgc ctgcggtcct tgaaggccga gatggcgagg agggcgatgg
     4981 agccgcccag ggcgaggatg aactcgaagg tcatggccgg gccgaagacg cggtcgacgc
     5041 cgagcttgtc cttcgcgaag aggaaggcga ggcttaaggc gaggccaatc ccgccccagg
     5101 ccaggaggat gggcttgcgc gcgcgggggc ggcccgtgta ggcggcgacg aaggcgggga
     5161 agaagaagag catgcctagg aagccggtct tggagatctt cttgaggagg agctcgggga
     5221 gggcgtcggt ctggaggctg atctccaggt aggccgccgc gaaggagagg cagaggaggg
     5281 cgaagaaggc gtaggagagg cgctcccgcc ccgagagggc gaggaagaag aagtagaggg
     5341 ccaggatgag ggcggcgaag gaggaggccg tcaccatctc gaccctgagg aagttcctga
     5401 ggaagaccga gcccgtcgcc ttgaggggcg tcgcgaggcg ggggaactcg agggggtagt
     5461 cctcgtcgcc ctggagggga tagaaggcga cgaggagctc gttgggtccg cccggcggcg
     5521 agaggagggc ctcgggcagg cggagagccg tcgagaggaa gatgttggag acgtagcgtt
     5581 cgcggtggag gccgacctca aggaggaggt ggccgttgag gctcagggcg aaggggtagt
     5641 cgctgggcgg gaggatgagg gagacgggcc ccgctccttc ctcgaggagg aaggaggcct
     5701 tgaggcagta gctcctccgc ccctcggggc tcgcggcgag ggggccttcc gcgtaggggg
     5761 cgaagcccgg atcggctgga tcggggaagg gccgctccgc gtcgtagggg gcggcgagga
     5821 aggagacctc cgcgagggcg gcgccctttt ccaaggcggc ccggtccagg ttctccaggg
     5881 ccgcgagcgg ggcggcggcg agaccggcga ggaggagggc gaggaggggg cggcgcttcg
     5941 aggacatcgg ggctccttgt gcgccgtcga ttctattcga ggaggcggag gcgcgccagg
     6001 gcgaaggccg gtcgacgcga cgaattcctc ataggccgta gcggcggcgg agcgcccgat
     6061 cgcgtgcagg cgagagggcc tagaagccga ggctgcggcg gagctccagg gcgaggaggg
     6121 aggcttcgcc ccccacgagg tagaggccgc ccgagccccg gcggaagggc ggggaaggca
     6181 gggtgtcagg cgggcaggag aggaggcggc ggacgaactc ggccttcctc cgcctggaga
     6241 ggggcggcag gccgcgcctg tagccgcgct cctcgtccca ggtgccgacg aagcggctgg
     6301 tctcaggcgg cgcctcgtca aagcctggcc gcgcgtcgtt gtcggggccg cccgggaagc
     6361 cggggccgcc gattcggccg agggcgcggc ctatccgggc ggcccaggag gcgaggcggc
     6421 gcagcctggc ccggcgccgc gcctcgtcga aggctctgta ggcggcatat tcccgctcct
     6481 cgcggggcag ccagtcctcg gcgaggaact ccgcgtggcc gatgccgatt ttccccagga
     6541 tgcccatggt cttctccttt acattcgata aatgcaaccg gttgcattcc ggtgccaaaa
     6601 aaaacctagg ccgaatcgcg ggcgacgagc tccacgggca cgacgcggtt cgtgacgacg
     6661 ccgcttccga ggaaggcgac gaggtcgcgc gcgaggagct tgccggtcag cgagatgtgc
     6721 tggctgatgg tcgtgagcga ggggctcacg taggccgcta ggcggaggtc gtcgtagccg
     6781 acgacggcga cgtcctcggg tatccgcagg cccctggcct tgaggacgcg catggctccg
     6841 atggccatga ggtcgctcgc ggcgaagacc gcgtcgagct tcgggtctgc cttgaggagg
     6901 cgctccatcg cgagggctcc cgattcctcg gagtagtcgc cgaaaacgag gcgctcgctc
     6961 tcgaggggaa ggccggcctc ggacaggagc tcggagaagc cgcgccgccg ctccccggac
     7021 tcgaggtcgt cctcggggcc gccgaggaag gcgatccgcc tcgccccccg tcccaggagc
     7081 ctgtccgccg cgagtcggcc gcccgaggcg ttgtccccgg tcacggaaca gtagcgcccc
     7141 tcgccgatgc cccaggcgat gaagggggcc tcccgctccg cgagcagctc gaggcggcgg
     7201 gacttctgct cgtaggtcat gaggatgaag ccggcgacga ggcccgagtc gaggtagtcg
     7261 gcgatccagc cctcgtcgcc gggctcgacg tagagggcga gcatgtcgta gccgagttcg
     7321 cggaggccct gggccacgcc gcccatgatc tccaggttga agaggtcggc cgagcctgag
     7381 ccggcgccgc agggcgagca gtcggaggtg acgaaggcga cggagcggct ggagcccagg
     7441 ctcaggtagc gggcggggag gttggggcgg aagccgtgct cccgggccag ggcctcgatg
     7501 cgctccctgg tctccgcgcc gacgaggggg ctgccggaga gggcgcggga gacggtcgac
     7561 ttggagacgc cggccagctt ggcgaggtcc gctatggacg cgagtttggg cttcatgctc
     7621 tgcaaccggt tgcaagaata cgtctgagat gcgctttcgt caagggccat ccggcggcac
     7681 gcggctccgc agctcggcga accatcgtgg gcgacccgcg ccgctccccg cggcgccctg
     7741 ggcttggagc ttgacctcgc ggatacgacc gaggagaatc ccccaatgcg ttcacgccgt
     7801 tcctgggcag cgggcaagcg cccgtcgtca aggctttccc tggccgcggc tctctgctcg
     7861 agcctcctcc tcctggactg ccgggaggcc ccggccaggg aggcgccggc tttccggcag
     7921 cagcctccct ctctgagcga ggcggagcgc gcctaccttg agcggctcgg gccggtcccc
     7981 ctctgcgtcg acagcgactg ggagccctac gagtacctgg acgaaaaggg cgagtacgcg
     8041 ggcatcgcgg cggacctggt caggctcatc gcggcccgct ccggggtgga gttccgcctg
     8101 ctgcggacca gggactgggc ggagagcctc gaggcttcca agaccggggc ctgcctggcc
     8161 ctcgccttcc tgaacgcctc tccggagcgc gagggctggc tcgtcttcac cgaaccctac
     8221 ttcagcgatc ccaacgtctt catcaccagg aacgggcacg actacatagc cgaccccgcc
     8281 cggctttccg gcgagaggat cgccctgcct gccggcacct ccgtcgagga gctggtgagg
     8341 gcccgctatc cgaacctgga ggtcgtcacg gtcgactccg agcgggaggc gctgcggctg
     8401 gtggattccg gcgaggcgga catgaccctg cgctccctga cgatggcggc ctatgtcatc
     8461 aggaaggaag gcctcttcaa cctcaggatc tcgggccagc tgcccggctt ggtgaacgag
     8521 ttcaggatgg gggtggccaa gggcgagcca ctgctgaagg acatcctgga ccgcggcgtc
     8581 cggagcctga gtccgcagga agtccaggcc gccgtgaacg cccacatcgc gatcgaggcg
     8641 cggaccgccg ccgacccgcg gcccctggcc tgggcgacag gggccttcgc cgtcctgtcc
     8701 ctcctctggc tcctgtggag cctggcccag cgcaactatc tcaagcacct gcgcttcgtc
     8761 ctggacgcga tgccggccta tgtctttgtg aaggacgcca ggggccgcta cctcctggcc
     8821 aaccgctgga tggcctcggc cttcggcgtg ccgccggagc gggtgaaggg actcaccgac
     8881 gccgactgcg gcggctccgc ggaggagatg gaagcctgga gggccgagga ccggcaggtc
     8941 ctggagagcg ggaagccgct gcgcatcgac aaccatcccg gcaggagggt ggacgggagc
     9001 cccggctggt tccagacgat caagatgccc taccggcgcc ccgacacggg caagaaggcc
     9061 atcctgggcg tcaccctgga cgtgacggac ctgaagaacg ccgagctcga gctggagcgg
     9121 cgggagcagc gcttccgcca cctggcccag cacgacggcc tcaccgacct gcccaacagg
     9181 ggactcttct cggaccgcct gggccaggcc atggccctgt gccggaggga ggggaccagg
     9241 ctggccctgg tcttcctgga cctggaccat ttcaaggaag tgaacgacat ccacgggcac
     9301 cgggcgggcg acctcctcct cacggaggcc gcgaggcgcc tcaaggccgc gatcagggcc
     9361 tccgacagcg cggggcggat cggcggcgac gagttcgtcc tcttcctcct ggacatcgag
     9421 gacagggcca gggcggcggc ggccgcggag aaggccaggg ccgcgatgga gctgcccttc
     9481 ctggtcgagg gggaagagct gcgcgtctcc gccagcatcg gcatagccct ctttcccgac
     9541 gacggcgagg acgagaccgc gctctcccgc cgcgccgacg cggcgatgta ccgctccaag
     9601 gccctggggc ggaaccgcgt gtcggtctac gatcccggac tggactcggg cgtcgaggcg
     9661 aagtagccgg tggccttgcc cctcgcccct cgcgccggcg tcagcctgaa tgggggcgga
     9721 agtagcggga ggcggggccg aggggcctgt cgcccgagac atgggcggaa aggtgctccg
     9781 cgaggcgggc gacggtctcc ggggggaggc gcacgtgggc catgcggacg agctcctcgc
     9841 ggacgcgctg ttccttggcg gcgccgacga aggcgaggac ctcgcggaga tcctcctccg
     9901 aggcggcgga gaggaggacg gcgaggtcgc ggtcggcggt gttgaggagc cgcgactggg
     9961 cgagcttggg cggagcagcg cgcagggcct ggaggaaggc cgagaccgcc tgggcgttcg
    10021 cggcggagac ccggctcacg cgcgggcctt gcgcggcgcg gagaggacac tggttgcggc
    10081 gctcatgcct caatgctagg ccgccagccc cgggcgcgca agcgccgcga gcgcgaggag
    10141 ctgcgtgggg gcagggcggg atcgcccgag ccgcccgggc cttcctgggg cggcgggccc
    10201 ggaagggacc gccgcctgag ggacgccccg cggcgaggga agcccgcccg agggggagcc
    10261 gcggcggctt tcggcgccgc cgcgcgcggg ggagggcgcg ggccaggggc tcgcgggcgc
    10321 tccccccttt ttttcctaga gcgccttctt gagatcctcg acgcggtcgg tgcgctccca
    10381 gctgaagtgg tcgcggccga agtggccgta ggcggcggtg cggaggtaga tcggcttctt
    10441 gaggtcgagc tcgcggatga tgccgccggg ggagaggtcg aagaccttct tcaccgccgc
    10501 ggcgatgcgc tcctccggga ccgtggaggt gccgaaggtc tccaccatca cggagacggg
    10561 gaagggcacg ccgatcgcgt aggcgagctc gagctcgcag cgctccgcga gaccggcggc
    10621 gacgacgttc ttggcgacgt agcgcgccat gtaggcggcg gagcggtcga ccttggaggg
    10681 gtccttgccg gagaaggcgc cgccgccgtg gcggcccatg ccgccgtagg tgtcgacgat
    10741 gatcttgcgg ccggtgaggc ccgtgtcgcc catggggccg ccgatgacga agcggccggt
    10801 ggggttcacg aagtacttcg tgtccttcat gtcgaggccg gtcggctcca ggatggggcg
    10861 gatgatctcc tcgatgacgg tgtcgcggag ggcctcgtag ggga
//
LOCUS       DAOOSJ010000063        10725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4028_length_10725_cov_6.096720, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000063 DAOOSJ010000000
VERSION     DAOOSJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_10725_cov_6.096720"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLG14_04645"
     CDS             complement(<1..688)
                     /locus_tag="PLG14_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX48454.1"
                     /translation="MAAIGQRGEAGELGPLFNPIDRALARLHLALFVRRKAGSVAGAV
                     LLYALVVLLLGERLAVSSNYFVFLPLLSAALAYGLPGGLAAGLLGLPSNLLLFRLIGH
                     PEYSPASKPIAWIFGAAVGLSLGYLSDYFRKLDREAERRAATAASLAKALEEKELLLA
                     ELHHRVKNNLNVIKSVVQLQKNRSSDPDFLAAADDLMGRVLAIAQVHERLYGEGRIEA
                     IEPRGYLEALV"
     gene            781..1176
                     /locus_tag="PLG14_04650"
     CDS             781..1176
                     /locus_tag="PLG14_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48455.1"
                     /translation="MQATILVVEDECLCGMEIQESLEKAGHRVPEVLSSGDEVLGAVL
                     RHKPDLVVMDINLKSYIDGIDAASRLRMVRPTPVIYVTAYPEKGTEDRARRTEPFAYL
                     QKPLEEGALVDCVERALAAAGGAAGAALC"
     gene            1170..1520
                     /locus_tag="PLG14_04655"
     CDS             1170..1520
                     /locus_tag="PLG14_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48456.1"
                     /translation="MLRRLSEADCRAAIREGDFLPGVAGAAPRVAVVLTQSWCPQWGW
                     MRSYLESLPEEPGVAVFYVEYDREPFFEDFMAFKEDVFGNREVPYVRYYREGRLSRES
                     NFIDRGGFLRLLSS"
     gene            complement(1608..2426)
                     /locus_tag="PLG14_04660"
     CDS             complement(1608..2426)
                     /locus_tag="PLG14_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX48457.1"
                     /translation="MIHSIDLPGHGRIHAESEGTGPAVVFVHADFVDGRMWDGVRALL
                     SARFRTAAYDKLGYGRSDPVTSPVVRRRELEAVVDALGLTAFHLVGCSNGGQQALDFA
                     LERPARVLSLALVNSSPSGWRPQGEMPPLLMEMFAAVQAGDFAAASELQLRIWFDGPD
                     RDQTRFSPEIREARRLSSVMNRIFVERGTFFQADPSLLEPPALSRLAEVAAPTLVVDG
                     LRDWAENRRANRVLAEGIPGARLVEVDGGHVAPMEDPAGFAALWESMVQARRVS"
     gene            2471..3469
                     /locus_tag="PLG14_04665"
     CDS             2471..3469
                     /locus_tag="PLG14_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOX48458.1"
                     /translation="MALNFANSKKRRDTLRADRLISLILELQDHGKTTAARLAAALGV
                     SRRTILRDLDALSLSGVPVLAEGGPGGGVWLDPAYRTSLTGLKEAELRTLLVAGDARL
                     LDDLGWGQAFLESQRKLRASVPRRFEPGVDHLRRRLVVDSRWWWHEAGEPNLEVLQAA
                     VWEDRVVEGDYQSWDGGAKAVRLEAYGLVAKAGLWYFVGRRDGEWRTYRVSRFASVKV
                     SGSFARDPLFDLSTWWPAHASEFAGEMAGFRFSLGLPEDRLRFLRGIAPGRVDVAGPW
                     ERPGWIRVDVGLDSSLYAELVVLGLGRQCVILDNPELAEAVVQRCREGLAAHEDPP"
     gene            3589..5094
                     /locus_tag="PLG14_04670"
     CDS             3589..5094
                     /locus_tag="PLG14_04670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOX48459.1"
                     /translation="MERKIRRLAAILLVAAALFAAAAGTAAAAPARSSAPAHKTLANG
                     LEVFVAENRAVPLATVCIVFRGGAVAQTPETAGLFHLYEHMLFTANEKYPNQAAFTAA
                     LNRMGVSSWNGGTGGEYINYYVTVPSDKLGEGLEFWSWAAKKPVFDPAKLESEKDVVI
                     NEIRGYHADPDQIMENALESRIFPAYPWRKNIDGPEENIRKATVADLEAMRRAYYIPR
                     NAALMIGGDVSPKEAFALAEKWFGEWEGGPAPAVGEPPQGPLAPGVRLAYADEEYYEG
                     VAGAGFRWRGPDVMRQTKDTYVADVLLFLLSSPVGRFKSALMEKGPGLYDPEYIGFGY
                     PTSRDGGVLSFSAFLVLEDPAKDGPTLDRVAALEKAVLAEFEAVAKDPAAYFGAEELA
                     KAKGKLIDQNLLSTEVASSYVTDTLTFWWAVATTDYFQGYERNCRAVSFSDISAFVSR
                     YLLGAPLASTLRMAGQAFRDEPGMGGRMKALGFEAVDESAAFWWQQAGKKE"
     gene            5099..6625
                     /locus_tag="PLG14_04675"
     CDS             5099..6625
                     /locus_tag="PLG14_04675"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOX48460.1"
                     /translation="MRNILSARRPAARAFALVLAASLAALACLPGCATAPASGRVGPG
                     ALSGPLATGPFPSPSELSPFAREGLSSLSTFELSNGIPVVLRQNGASPVRHLSLVLRG
                     GSLAAEPATAGFELLAMRTMARGSKGYSYEDITSLLDETSAAMGAAAAFEYSTYSLTA
                     LDKHFARLLPVWADTLAAPRFSEEDFAQVLSEAKLALKSREQDPWQRTGLVMNAEFFA
                     GHPYAASPDGTKESLAAASLQAAKDWYAKSFSADRIFVVAVGDFDAEELKAGLEKALG
                     ALPDRKAGLPAPAPAFAAAGEGRLVKSEHAQSKGVGYLRGDFAAPPPSDPSYMAANVA
                     MKMFSDLLFNVVRDEHGAAYSPSAYVRGFGANYGSVAVFKTTKAAEVKAYIDEAAAEL
                     AAGRCLSVDPARSEGGKPRMPLAEALPAYKAQFANDYYEKLQTNAAVAGLIAQSVATT
                     GDCRSWLLDGARIAAVGPEDVARAFKAVLDSRVTWVVLGSKDVIEPVDPAAFGKLGPQ
                     "
     gene            complement(6727..7179)
                     /locus_tag="PLG14_04680"
     CDS             complement(6727..7179)
                     /locus_tag="PLG14_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOX48461.1"
                     /translation="MREPLGLLWAALGASGFAVLFDLRLREVPFAAAGAAGGWWLYSA
                     VGAASSEAAGYFAAAAFIGLYAEVVASLRKRPAAVYIVSAILPIVPGGGMYYTMLNSV
                     RGDLYASLGTGFETLQAAGAIAAGLAVSSALSRLVSLDRLARRLKGKA"
     gene            complement(7176..7967)
                     /locus_tag="PLG14_04685"
     CDS             complement(7176..7967)
                     /locus_tag="PLG14_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOX48462.1"
                     /translation="MPQTERLPDPEEILLLAADAARVVLESGGETYRAEETAAAVCSA
                     LGGVEAECFATPTGIQLSFCGLDGKVRSIVRRVKTRSMNLDRQARLNALSRDLYAGRL
                     DFDGAARELDLIERRRGYGLPVSLTAAAVGTASFTLLFGGRWNDALVAAAVGALLSRL
                     TRRLARRGIADFFTNIVGGAVAALLCLGAERLGLAASADTTIIGVIMLLVPGVAITNA
                     IRDTIAGDLVAGVARGADAFMAAAAISIGAGGAFEAWRFLGMGPL"
     gene            8076..>8676
                     /locus_tag="PLG14_04690"
     CDS             8076..>8676
                     /locus_tag="PLG14_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48463.1"
                     /translation="MGRQAFRGSRTSYLALALLALVVVGGAWTAADRATRKAQLALAA
                     RNRALEVELARSAMQEGFLRILEENRILANYSFPEYLRGARSSASMVALLASELESYK
                     ESLAYAFFEKPGETRFLGAAPGADGAVEALKAASFEAWERLAAFEGPLVIPARGSGPE
                     PYFMVFYPARVEGERAGLLGTAVGFGKAILKYLQPLRSGN"
     assembly_gap    8677..8776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8777..10174
                     /locus_tag="PLG14_04695"
     CDS             <8777..10174
                     /locus_tag="PLG14_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX48464.1"
                     /translation="VGFGKAILKYLQPLRSGNGRGAFLLSADGEVLWPSEAYGPLAPD
                     EEDSAIVTRRDFRLGDATFSVVGADDKTALLAELRSIEAPRLLVIASGFALVVLALLA
                     VNRLSAAERQRSAATEEGERLAAAVAERERQLAESELRYSAVFEGASDGVLIFDERDV
                     IQSCNPRAAALFGLGREELVGRKVGELSPPVQPDGEASAEKGARLLALVWAEGRPRSF
                     EWTHRAKDGREFIVEVSLAPLDFRSQRLSLAFLRDVTERKRNERALGEALEDRELLLH
                     ELHHRVKNNLQFLDSLIELQKGVEGEASREALSKMQSRVSSLAAAYLVAADRPESLKI
                     GSRAYIGAVCSIVRDGAAARGIRFLMEIDCDDIPLSFDVAVPIGLILRELLANAALHG
                     ACGDGTSRARLRFSRAGNEALLEVGDWGCGLAQGAEEGLGLTIARALARQLGGSLELV
                     PADPGLVARARFPLA"
     gene            complement(10171..>10725)
                     /locus_tag="PLG14_04700"
     CDS             complement(10171..>10725)
                     /locus_tag="PLG14_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48465.1"
                     /translation="GVVAALNLDSGTGTASLVTNILGASLSFPLAPASRLSFEPGADL
                     YWAYYGEVANRAVPIELENRDAFVLALLLDAPLSYAIPLSEDFRLSLGAGLAFDLRVG
                     IKAAEEVADETVAAINSYLWSKGRFLMPSTFLRIEYRLTENFDFGFAARAFWPLYNLW
                     AGEGLPILDQGIFGGSLVVRQRLR"
BASE COUNT         1550 a         3773 c         3813 g         1489 t
ORIGIN      
        1 cgaccagggc ctcgaggtag ccgcggggct cgatggcctc gatccgcccc tcgccgtaga
       61 gacgctcgtg gacctgggcg atggccagga cgcgccccat gaggtcgtcc gccgccgcga
      121 ggaagtccgg gtcgctcgag cggttcttct ggagctggac gacgctcttg atgacattga
      181 ggttgttctt gacgcggtgg tggagctccg cgaggaggag ctccttctcc tccagggcct
      241 tcgcgaggga ggccgcggtg gcggcccggc gctccgcctc gcggtcgagc ttgcggaagt
      301 agtccgagag gtagccgagg gagaggccga ccgcggcccc gaagatccag gctataggct
      361 tgctcgcggg cgagtactcc ggatggccta tgaggcgaaa gaggaggagg ttcgaaggca
      421 ggccgaggag gccggcggcc aggcctccgg ggaggccgta ggcgagggcc gccgagagga
      481 gggggaggaa gacgaagtag ttgctggaga cggcgagcct ctctccgagg aggaggacga
      541 cgagggcgta gaggaggacc gctcccgcta cgcttcccgc cttccgccgg acgaagaggg
      601 cgaggtggag ccgcgcgagg gcccggtcga tggggttgaa gaggggtccg agctcccctg
      661 cctcgccgcg ctgtccgatc gccgccatgc tccctcccgc gcccgccgga agagggcgcg
      721 ggaccatagt atacgcggcg gggggatcgt gctactcttc cgcccggaga gccaacagcg
      781 atgcaagcca ccattctcgt cgtcgaggac gagtgcctct gcggcatgga gatccaggaa
      841 agtctcgaga aggccggcca ccgcgtgccc gaggtcctgt cctcgggcga cgaggtcctg
      901 ggcgccgtcc tccgccacaa gcccgacctg gtcgtgatgg acatcaacct gaagagctac
      961 atcgacggca tcgacgccgc ctcgcggctg cgcatggtgc gcccaacccc ggtcatctac
     1021 gtcacggcct atcccgagaa aggcacggag gacagggcgc ggaggaccga gcccttcgcc
     1081 tacctccaga agccgctcga ggagggcgcc ctcgtcgact gcgtcgagag ggccctcgcg
     1141 gccgcgggcg gcgcggcggg cgccgcacta tgctgaggcg cctttccgag gccgactgcc
     1201 gcgccgcgat ccgggagggc gacttcctcc ccggcgtggc gggagcggct ccccgcgtcg
     1261 ccgtcgtcct cacccagtcc tggtgccccc agtggggctg gatgcgctcc tacctcgagt
     1321 ccctgcccga ggagccgggc gtcgcggtct tctacgtcga gtacgacagg gagcccttct
     1381 tcgaggactt catggccttc aaggaggacg tcttcgggaa ccgcgaggtg ccctacgtcc
     1441 gctactaccg cgagggcagg ctctcccgcg agtccaactt catcgaccgc ggcggcttcc
     1501 tccgcctcct ctcctcctag ctcctcaggg ctccggtctg cctccccggc cgcgcgccta
     1561 cggcggccgc ggcgccaccg gcctccgcgg cgccggactt ggctccttca ggaaacgcgc
     1621 cgcgcttgga ccatgctctc ccacagggcc gcgaagccgg ccggatcctc catgggcgcc
     1681 acgtggcctc cgtcgacctc gaccagcctg gctccgggaa ttccctcggc caggacgcgg
     1741 ttggcccggc ggttttcggc ccagtcccgg agcccgtcga ccaccagcgt gggcgccgcc
     1801 acctcggcga gccgggacag ggccggaggc tccaggagag agggatcggc ctggaagaag
     1861 gtgcccctct cgacgaaaat gcggttcatg acggaagaga gccggcgcgc ttcccggatt
     1921 tccggagaga acctggtctg gtccctgtcg gggccgtcga accagatcct cagctggagt
     1981 tcggatgcgg ctgcgaagtc ccccgcctgg accgcggcga acatctccat gagaagcggg
     2041 ggcatttccc cctggggccg ccagcccgag ggcgaggaat tcaccaaggc gagggacagc
     2101 acccgagccg ggcgctccag ggcgaagtcc agggcctgct ggcctccgtt ggagcaaccc
     2161 acgagatgga aggccgtgag gccaagggcg tcgacgacgg cctccagttc ccggcggcga
     2221 accaccgggc tcgtgacggg atcggagcgg ccgtagccca gcttgtcata ggccgccgtt
     2281 cggaagcggg ccgagagcag ggcccggacg ccgtcccaca tgcggccgtc gacgaagtcg
     2341 gcatggacga atacgacggc ggggccggtc ccttcggatt cggcgtggat ccggccgtgg
     2401 ccgggaagat cgatggaatg gatcatgtgt actccttgac gctatactgc tccagggtgg
     2461 gtgacaactt atggcactca atttcgcgaa ttccaaaaaa aggagggaca ccctgcgggc
     2521 cgatcgactc atatccctga tcctggagct gcaggaccac ggcaagacca cggcggcgcg
     2581 cctggccgcc gccctggggg tcagccgccg gaccatcctg cgcgacctgg acgcgctttc
     2641 cctatcgggg gtgcccgtcc tggccgaagg cgggcccggc ggcggggtct ggctggaccc
     2701 cgcgtaccgg acctcgctga cgggcctgaa ggaggccgag ctgcggacgc tgctggtggc
     2761 cggcgacgcg cggctgctcg acgacctggg ctggggacag gccttcctcg aatcccagcg
     2821 caagctgagg gcctcggtgc cgcgccgctt cgagcccggg gtggatcacc tgcggcgacg
     2881 cctggtcgtc gactcccggt ggtggtggca cgaggcgggg gagccgaatc tcgaggtcct
     2941 ccaggccgcc gtgtgggaag accgggtggt cgagggcgac tatcaatcgt gggacggcgg
     3001 cgcgaaggcc gtccggctgg aggcctacgg cttggtcgcc aaggccgggc tctggtactt
     3061 cgtggggcgc cgggacgggg aatggcgcac gtatcgggtg tcgcgcttcg cttccgtcaa
     3121 ggtttcgggg tcctttgcca gggatccgct gttcgatctc tcgacctggt ggcccgccca
     3181 cgcctccgaa ttcgccgggg agatggcggg cttccggttc tcgctgggcc tccccgaaga
     3241 ccggctgcgg ttcctgcgcg gcatcgcccc gggccgcgtc gacgtggccg ggccctggga
     3301 gcgccccggc tggatcaggg tggacgtggg actcgactcg tcgctctacg ccgaactcgt
     3361 ggtgctgggc ctcggccggc agtgcgtgat cctcgacaat ccggagttgg cggaagcggt
     3421 ggtccaacga tgcagggagg gtctggccgc ccacgaagac ccgccgtgac gggaggcgtt
     3481 cggggagtcc cggcgcttcc cgggcgcggt ccgcaccgcg ccgccgcttc ttgactcccc
     3541 gccccctccc gctatactcg ggccaacggc atccaaaaag gaggcatcat ggagcggaaa
     3601 atccggaggc tggcagccat cctgctcgtg gccgcggccc tcttcgccgc ggcggcgggg
     3661 acggccgccg ccgcccccgc gcgctcgtcc gcgcccgcgc acaagaccct ggccaacggc
     3721 ctggaggtct tcgtcgcgga gaaccgcgca gtccccctcg cgaccgtctg catcgtgttc
     3781 cggggcggcg ccgtggccca gacgccggag acggccggcc tcttccacct ctacgagcac
     3841 atgctcttca ccgcgaacga gaagtacccg aaccaggccg ccttcaccgc cgccctgaac
     3901 cgcatgggcg tctccagctg gaacggcggc acgggcgggg aatacatcaa ctactacgtc
     3961 accgtcccct ccgacaagct aggcgagggc ctcgagttct ggtcctgggc ggccaagaag
     4021 cccgtcttcg accccgccaa gctcgagagc gagaaggacg tcgtcatcaa cgagatcagg
     4081 ggctaccacg ccgatcccga ccagatcatg gagaacgccc tggagtcgcg gatcttcccc
     4141 gcctacccct ggaggaagaa catcgacggg ccggaggaga acatcaggaa ggcgaccgtc
     4201 gccgacctgg aggcgatgag gcgggcctac tacattccga ggaacgccgc cctgatgatc
     4261 ggcggcgacg tctccccgaa ggaggccttc gccctggccg agaagtggtt cggcgagtgg
     4321 gagggcgggc cggcgcccgc cgtcggcgag ccgccccagg ggccgctggc gcccggcgtg
     4381 cgcctggcct acgccgacga ggagtactac gagggcgtcg cgggggcggg cttccgctgg
     4441 cgcggccccg acgtcatgcg gcagaccaag gacacctacg tcgcggacgt cctcctcttc
     4501 ctcctctcct cgcccgtcgg ccgcttcaag agcgccctca tggagaaggg tcccggcctc
     4561 tacgaccccg agtacatcgg cttcggctac ccgacctcgc gggacggagg cgtcctctcc
     4621 ttctcggcct tcctcgtcct ggaggacccc gcgaaggacg ggccgaccct ggacagggtc
     4681 gccgccctgg agaaggccgt cctcgccgag ttcgaggccg tcgcgaagga ccccgcggcc
     4741 tacttcgggg ccgaggagct cgcgaaggcc aagggcaagc tgatcgacca gaacctcctc
     4801 tccaccgagg tggcgagctc ctacgtcacg gacaccctca ccttctggtg ggccgtcgcg
     4861 acgacggact acttccaggg ctacgagagg aactgccgcg cggtctcctt ctcggacatc
     4921 tccgccttcg tctcgcgcta cctcctcggg gcgcccctcg cctccaccct gcggatggcc
     4981 gggcaggcct tcagggacga gcccgggatg ggcgggcgga tgaaggccct gggcttcgag
     5041 gccgtggacg agagcgccgc cttctggtgg cagcaggccg gaaagaagga gtagagccat
     5101 gcggaacatc ctgtcggcgc gccgccccgc ggcgcgggcc ttcgccctcg tcctcgcggc
     5161 ctccctggcc gcgctcgcct gccttcccgg ctgcgccacc gcgcccgcgt cgggacgcgt
     5221 cgggccggga gccctctcgg gccccctcgc caccgggccc ttcccctcgc cctccgagct
     5281 gtcgcccttc gcccgcgagg ggctctcgag cctctcgacc ttcgagctct ccaacggcat
     5341 ccccgtcgtc ctgcggcaaa acggcgcgag cccggttcgg cacctctccc tggtgctccg
     5401 cggcggctcc ctggccgcgg agcccgcgac ggcgggcttc gagctcctgg ccatgcggac
     5461 gatggcccgc ggctccaagg gctactccta cgaggacata acgtcgctcc tggacgagac
     5521 gagcgcggcg atgggggccg cggccgcctt cgagtactcg acctactccc tcacggccct
     5581 ggacaagcac ttcgcccgcc tcctccccgt ctgggcggac accctggccg ccccccgctt
     5641 ctccgaggag gacttcgccc aggtcctctc ggaggccaag ctcgccctca agtccaggga
     5701 gcaggatccc tggcaaagga cgggcctcgt gatgaacgcc gagttcttcg cgggccaccc
     5761 ctacgccgcc tcgcccgacg ggaccaagga gtccctggcc gcggcgagcc tccaggccgc
     5821 caaggactgg tacgcgaaga gcttctccgc ggacaggatc ttcgtcgtcg cggtgggcga
     5881 cttcgacgcg gaggagctga aggccgggct cgagaaggcc ctgggcgccc tgcccgaccg
     5941 gaaggccggc ctccccgcgc cggctcccgc cttcgccgcg gcgggcgagg ggcggctcgt
     6001 gaagagcgag cacgcgcagt cgaagggcgt gggctacctc cgcggcgact tcgccgcgcc
     6061 gcccccctcg gacccctcct acatggcggc caacgtcgcg atgaagatgt tctcggacct
     6121 gctcttcaac gtcgtgcgcg acgagcacgg cgccgcctac tcgccctccg cctacgtccg
     6181 gggcttcggc gccaactacg gctccgtggc ggtcttcaag acgaccaagg ccgccgaggt
     6241 gaaggcctac atcgacgagg ccgcggccga gctggccgcc gggcgctgtc tctccgtgga
     6301 ccccgccagg tcggaaggcg ggaagccgcg gatgccgctc gcggaggcgc ttcccgccta
     6361 caaggcccag ttcgcgaacg actactacga gaagctgcag acgaacgccg cggtcgcggg
     6421 cctcatcgcc cagtcggtcg cgacgacggg cgactgccgg agctggctcc tggacggcgc
     6481 gcgcatcgcc gccgtggggc ccgaggacgt ggcccgcgcc ttcaaggccg tcctcgacag
     6541 ccgcgtgacc tgggtggtcc tgggctccaa ggacgtcatc gagccggtgg acccggcggc
     6601 gttcgggaag ctcgggccgc agtagccgcc gcgcccggcc gtcccgaggc cgcccttgag
     6661 ggcggccttt ttcgcgccct cccggcgagg cgccgtccag cggcgcccct ccccctcggc
     6721 cgtcagctag gccttgccct tgagccgcct cgccaggcgg tccagggaga cgaggcgcga
     6781 gagggcgctc gagacggcca ggccggccgc gatggcgccc gccgcctgca gggtctcgaa
     6841 gccggtgccc aggctcgcgt agaggtcgcc gcgcacggag ttgagcatcg tgtagtacat
     6901 gcccccgccg ggcacgatgg ggaggatggc gcttacgatg tagacggcgg cggggcgctt
     6961 gcgcagggag gcgacgacct ccgcgtagag gccgatgaag gcggccgctg cgaagtagcc
     7021 ggcagcctcc gagctcgcgg cgccgaccgc ggagtagagc caccagccgc cggccgcccc
     7081 ggcggcggcg aagggcacct cgcggagcct gaggtcgaag aggacggcga agcccgaggc
     7141 gccgagggcg gcccagagga ggcccagggg ctccctcata gcggccccat cccgaggaag
     7201 cgccaggcct cgaaggcgcc gccggccccg atggagatcg cggccgccgc catgaaggcg
     7261 tctgcccccc tggccacgcc ggcgaccagg tcccccgcga tggtgtcgcg tatcgcgttg
     7321 gtgatcgcga cgccgggcac gaggagcatg atgaccccga tgatcgtcgt gtccgcgctc
     7381 gcggcgaggc ccaggcgctc cgcccccagg cagaggaggg ccgccacggc cccgcccacg
     7441 atgttcgtga agaagtccgc tatgccgcgc ctcgccagcc gcctcgtgag gcgggagagg
     7501 agggcgccaa cggctgcggc gaccagggcg tcgttccagc ggccgccgaa gaggagggtg
     7561 aaggacgcgg tgcccacggc cgcggcggtg agggagacgg ggaggccgta gccccggcgg
     7621 cgctcgatga ggtcgagctc ccgcgcggcc ccgtcgaagt cgaggcggcc ggcgtagagg
     7681 tcgcgcgaca gggcgttgag cctggcctgc cggtccaggt tcatggaccg cgtcttcacc
     7741 cggcggacga tcgacctgac cttgccgtca aggccgcaga aggagagctg gatgcccgtg
     7801 ggcgtcgcga agcactcggc ctcgacgccg cccagggccg agcagaccgc cgcggccgtc
     7861 tcctccgcgc ggtaggtctc gcccccgctc tccaggacga cccgcgccgc gtccgcggcc
     7921 aggaggagga tctcttcggg atcgggcagg cgttcggtct gcggcatggg aaaagcatag
     7981 cgcggatcgc ggctgattgg aaagaagcgc ctcgggcggc gcgccggccc gcgctttttc
     8041 ttgcggaagc cgcctcaggc gagtatcttg tagtcatggg aaggcaggcg ttccgcggct
     8101 ccaggaccag ctacctcgcc ttggccctcc tcgccctcgt cgtcgtgggc ggcgcctgga
     8161 cggctgcgga ccgggcgacc aggaaggccc agctcgcgct ggcggcgcgg aaccgcgccc
     8221 tggaggtgga gctggcccgg agcgcgatgc aggagggctt cctccggatc ctggaggaga
     8281 accgcatcct cgccaactac tccttccccg agtacctccg cggcgcgagg agctccgcct
     8341 cgatggtcgc cctcctcgcg agcgaactcg agtcctacaa ggagtccctg gcctacgcct
     8401 tcttcgaaaa gcccggggag acgcgcttcc tgggcgcggc gccgggagcg gacggggctg
     8461 tcgaggcgct gaaggccgcc tccttcgagg cctgggagcg cctcgcggcc ttcgaagggc
     8521 cgctggtcat ccccgccagg ggctccggcc cagagcccta cttcatggtc ttctaccccg
     8581 cgcgggtgga gggcgagagg gcgggcctcc tgggcaccgc cgtgggcttc ggcaaggcca
     8641 tcctcaagta cctccagccc ctccgctcgg ggaatgnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnngtgg gcttcggcaa ggccatcctc aagtacctcc agcccctccg
     8821 ctcggggaat ggccgagggg ccttcctcct ttccgctgac ggcgaggtcc tctggccctc
     8881 cgaggcctac gggcccctcg cgcccgacga ggaggactcc gcgatcgtga cgaggcgcga
     8941 cttcaggctc ggggacgcga ccttctccgt cgtgggcgcg gacgacaaga cggccctcct
     9001 cgccgagctc cgctccatcg aggcgccgcg gctcctcgtc atcgcctcgg gcttcgccct
     9061 cgtcgtcctg gccctcctgg ccgtcaaccg gctctccgcg gccgagcgcc agcggagcgc
     9121 ggccacggag gagggcgagc ggctcgcggc ggccgtcgcg gagcgggaga ggcagctcgc
     9181 ggagtcggag ctccgatact cggccgtctt cgaaggggcg agcgacggcg tcctcatctt
     9241 cgacgagcgc gacgtcatcc agagctgcaa tccccgggcg gccgccctct tcggcctggg
     9301 ccgggaggag ctggtcggcc gcaaggtcgg cgagctctcg cctcccgtcc agcccgacgg
     9361 ggaggccagc gcggagaagg gcgcgaggct ccttgccctc gtctgggccg aggggcgccc
     9421 gcggtccttc gagtggacgc accgggccaa ggacggcagg gagttcatcg tggaggtgag
     9481 cctcgcgccc ctcgacttcc gctcccagcg cctctccctg gccttcctcc gcgacgtcac
     9541 cgagcgcaag cggaacgagc gggccctggg cgaggccctg gaggaccggg agctcctcct
     9601 ccacgagctc caccaccgcg tcaagaacaa tctccagttc ctggacagcc tgatcgagct
     9661 gcagaagggc gtcgaggggg aagcctcccg cgaggccctc tccaagatgc agtcccgggt
     9721 ctcctccctg gcggcggcct acctggtcgc ggccgacagg ccggagagcc tcaagatcgg
     9781 ctcgcgcgcc tacatcggcg cggtctgctc catcgtgcgc gacggggccg cagcccgcgg
     9841 catccgcttc ctgatggaga tcgactgcga cgacattccc ctctccttcg acgtcgccgt
     9901 gcccatcggc ctcatactcc gcgagctcct ggccaacgcg gccctccatg gggcctgcgg
     9961 cgacggaacg agccgggccc ggctccgctt ctcccgcgcg gggaacgaag ccctcctcga
    10021 ggtaggcgac tggggatgcg gactggccca gggcgccgag gagggcctgg gactcaccat
    10081 agcgagggcc ctggcgcggc agctcggggg aagcctggag ctcgtcccgg ccgatcccgg
    10141 cctcgtcgcg cgggcgcgct tccccctcgc ctagcggagg cgctggcgga ccacgaggga
    10201 tccgccgaag atgccctggt cgaggatggg caggccctcg cccgcccaga ggttgtagag
    10261 gggccagaag gcgcgggcgg cgaagccgaa gtcgaagttc tcggtgaggc ggtactcgat
    10321 cctgaggaag gtcgagggca tgaggaagcg gcccttggac cagaggtagg aattgatggc
    10381 ggcgacggtc tcgtccgcca cctcctccgc ggccttgatg ccgacgcgga ggtcgaaggc
    10441 caggcccgct ccgagggaga ggcggaagtc ctcgcttaag gggatggcgt aggagagggg
    10501 cgcgtcgagg aggagggcga ggacgaaggc gtcgcggttc tcgagctcga tgggcacggc
    10561 ccggttcgcg acctcgccgt agtaggccca gtagaggtcg gcgccgggct cgaaggagag
    10621 gcgcgaggcg ggtgcgaggg ggaagctcaa ggaggcgccg aggatgttcg tgacgaggct
    10681 cgccgtgccg gtgcccgagt ccaggttgag cgccgccacg acgcc
//
LOCUS       DAOOSJ010000064        10721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4030_length_10721_cov_6.513032, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000064 DAOOSJ010000000
VERSION     DAOOSJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_10721_cov_6.513032"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2210)
                     /locus_tag="PLG14_04705"
     CDS             complement(156..2210)
                     /locus_tag="PLG14_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48466.1"
                     /translation="MVYLLIPLLLLSAPFLASSAPVGDVGLTVLPSLSIGLGPVVDGD
                     LPLFGMGGGATLRGEFRPGFAPYLLGRAAFDFDYLPFNGAEGGLSVLSGGASLGLAFD
                     PIPRLSLRLAGGGGLYMAMSELGSVRNPFLEAGAEVQLRLSPTLGLGLGGRYRRLLAP
                     SGSLYEGVSVQLGLGFDLAGARKGTELRMEPRLQPIFPLFYSYYDKNPAGEVLVSNGE
                     SSPYEKVSISFYAKQYMDGPRLCAELGSLAGGETRKVPVYALFNDAIFRVTEGTKAAG
                     EVIVDYYYLGKARKLSFPVTLQVQNRNAMSWDDDRKAAAFVTAKDPLVLGFAKNVAST
                     VRYEGAPPVSEDFRTALALFQTLKLYGLGYAVDPKTPYTALSTAETAVDFLQFPNQTL
                     SYRAGDCDDLSVLYAALLESVGVAAAFVTTPGHIFVAFDTGLSEGAAKRLFAPQTLVV
                     REGRAWVPVEVTLVKEGFVRAWRVGAQEWSQAEAAGQGGFYPVREAWKLYEPVGFVEG
                     GIAVSIPSPERIVDGYRKEMRLFLDAAVGPRVAEALSSERASGRSAEQAANRVGILYA
                     QFGLLDKAEEQFRSAARSGGYAPALTNLGNALYLKGDMKGAYEQYARALALAPDSAVS
                     LSGAARALYALQDYEKMNKALDRLRVVSPELAAKIDLQGGAVTTARASEAVSREVDAW
                     DE"
     gene            complement(2398..3561)
                     /locus_tag="PLG14_04710"
     CDS             complement(2398..3561)
                     /locus_tag="PLG14_04710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX48467.1"
                     /translation="MSASERHAFLITEAEAARDYGTTVLRAARHRHCAPREAGATALA
                     DELGRRPEIEVLALLDAEGRPEGLVSREGLFALLGKPFGREVLGRTRAWEIAERAPLL
                     DAHSGLFSAGARAGGEAPYRILVDAEGRFRAILSSRDLAEHLSRITEDDIELAGRIQE
                     RLEEGNEAVGGEGYRFEAWSRPSKGVGGDFWFSKRLEDGSAFVALCDVSGKGVAASLV
                     VSLVWGMLRMHDYGKGLASLLVALNEALVATFHLEKYLTGFFCVFDPRSGALEAADMG
                     HSHALVLREGKARRLKAPERNLPIGVEPELRPAIARWSLLSGDALLVYSDGLSEQENP
                     AGEEFGERRLAACALAALARGESLREALPAALDRHRGTTPQQDDMSFMLLNLS"
     gene            3695..4006
                     /locus_tag="PLG14_04715"
     CDS             3695..4006
                     /locus_tag="PLG14_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX48468.1"
                     /translation="MEWTPVATESGDGAEIRLVGDFDLYHAPLFSLFVLGKIEGGWRT
                     VLLDLSGVGYLDSTGVGSIIRIAQALRKKGTKLRFRGLSGTPRRVLEMANILALVEET
                     K"
     gene            4020..4553
                     /locus_tag="PLG14_04720"
     CDS             4020..4553
                     /locus_tag="PLG14_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48469.1"
                     /translation="MKGLIVRKLIVDERNELFDAQGMRLVEFPSDFGRVREYAAKVLE
                     DCPESFREGNLLEQQLSEIIKNGIKHGNRRDPSKRLRVWYDLRKRVRFIVEDEGEGFA
                     ELDAWNEFYRKRQEALFAEDFDAFLAMASYRGPRSDETDGGNSLVAALEYWNGGMIYN
                     AAKNKVGVVRWFSGGSD"
     gene            4643..5719
                     /locus_tag="PLG14_04725"
     CDS             4643..5719
                     /locus_tag="PLG14_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HOX48470.1"
                     /translation="MKFKEFLASYSAFLASSPREELKEGSRYVFLSDLHLGDGGSRDD
                     LAPNRGLVQNMLSRYYLERGYTLVLNGDIEDLSKFRYTDIRAAWSSLYAVFDAFAKEG
                     RLRKLVGNHDLGLLRIKDHPYELSHGLVLERGRDRLFVFHGHQASKFFTRHDYLSDFI
                     VRYLAKPLKIRNTSVDDDSKRRFKTERRIYRASRALGVVAVTGHTHRPLFESLSKYDS
                     LRWAIEELVAEYPLASGPRREAVAEMVELYRDELSRLGKRELAFDLSRSLYDRGSLLV
                     PCLFNSGCATGKHGATAIEYAGGELALVHWTGKKGPRPYVEREALMKDRIEGTPYARY
                     ILRSSPASHVFARVELLGRGKAGK"
     gene            5895..6389
                     /locus_tag="PLG14_04730"
     CDS             5895..6389
                     /locus_tag="PLG14_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOX48471.1"
                     /translation="MGRVLVTYYSASGGTRGAAEAIASVLSERGAAVDCATLGEARDV
                     EGYDAFVIGAPVNGFRWRPEALAFVELHRAVLAGKPTGLFLLSIMYGLGRPSARRRAL
                     SFLDPALALLPRAKAGYFGGTMQGPAPWPLRLAFGVKKDAPLDSRDWPAIKAWAAEFA
                     KELA"
     gene            complement(6439..6870)
                     /locus_tag="PLG14_04735"
     CDS             complement(6439..6870)
                     /locus_tag="PLG14_04735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX48472.1"
                     /translation="MDASALETVVTLKNQKVLFEGVSATNPGRPVQIDYLPPAGDGEG
                     FRGLELLLLAFGGCVSTCIVFLLRKSGKRVESYSMRAAGEKREKPLSLAKIRVEVELD
                     SPDTSEAELAAFVKTAKSLSPVWLAIAGNVEVEVAARVAVA"
     gene            complement(6918..7718)
                     /locus_tag="PLG14_04740"
     CDS             complement(6918..7718)
                     /locus_tag="PLG14_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX48473.1"
                     /translation="MACAGQGIEYREQPPCQALEGLVYCYWSFSSPRRPLSFSYRAVP
                     DLCADLYFDLSRFQRLKLLGIATRAIAAPMLPGSDFFGIRFAPGAIGRFFRLPLSEFQ
                     DRSPSFSELGGNAVAELEGRLAEARSAAARVAVAEAWLLARAGRGLRDADERFAKALG
                     NIYLHGGTKPVALLAEDARLSVRQFSRLFLEGQGLPPKAFSRIVRFQRALDLVLRHPE
                     LAGSRAAQEAGYYDQPHFLREFREICGELPSNVRNVQAVSRAREDTAS"
     gene            7963..8943
                     /locus_tag="PLG14_04745"
     CDS             7963..8943
                     /locus_tag="PLG14_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HOX48474.1"
                     /translation="MKKTILVAALALAAVASFAQGKFAWIDPVSDDKDKILPGVDPTK
                     VAGTIVTAGSSTVFPLSEALAERFKKEGFMGQVTIDSIGTGAGFERFAKTGETDIANA
                     SRAIKSSEADWAKALASPRTPIAFQVGTDALAVCVSAKNKFVQDATVKELALIFSTAQ
                     NWSDVRPTWPKKEIKRFSPGTDSGTFDYFVEHVFAKKKDGILAAKNLQLSEDDNVLVQ
                     GIAGDEYAIGYFGFAYAVENKGKIRPISVDGVEPNQANVDANKYPLSRPLFLYSDARI
                     IAEKPQVAAFLTYYLTYSNELIKKVGYFPAPADATRKSKQALADAMKGRY"
     gene            8950..9885
                     /gene="pstC"
                     /locus_tag="PLG14_04750"
     CDS             8950..9885
                     /gene="pstC"
                     /locus_tag="PLG14_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOX48475.1"
                     /translation="MSDTGSYPEGKTGTASLARRARPGEALVQSFLFFCAAVSILTTV
                     GIVVTLGEESFSFFKGVNLLEFFTGKVWQPAIERFGILPLLAATLTTSLYAMLVALPL
                     GLLVAVYLSEYASARVRDVLKPVLEVLAGIPTVVYGYFALTTVTPFLRSLIGEDVVEV
                     YNTLSAGLVMGVLILPLIASMCEDALSSVPAALREGAYAMGATKLEVSLRVVVPGAFS
                     GLAAAFILGLSRAVGETMIVALAAGAGPNLTLNPFRSAETMTGHIVRISGGDLSYDSV
                     DYKSLFAIAMVLFLVTLGLNMLSRRIIRRYQEVYE"
     gene            9882..>10721
                     /locus_tag="PLG14_04755"
     CDS             9882..>10721
                     /locus_tag="PLG14_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48476.1"
                     /translation="MSAPAKPQAALIPGEPADFSPNLDARKAKGAKWRVAFLGSTILS
                     VLFLFALVASIVNQGFGYVLTVAEVEPASLERDGVPLERQDVAALVETIRDNVPRRFF
                     IKVDAEAPLAQRSREELLAFVEAEVVKARVVGTWSLAESLLAKERILAFKEAEAPEGR
                     LFFRSWVNRALLSNPQSSTAMLAGLRGAVIGSLLTILFTIALALPLGIGAAIWLEEYA
                     KDNRLNRVIRTNIYNLAGVPSIIYGMLGLALFVRALEPLTSGSMFQAAGADSSGGANG
                     RTIL"
BASE COUNT         1726 a         3713 c         3688 g         1594 t
ORIGIN      
        1 ctccaaatac ctgtcgttcc ccttcatcac ctcgaccgtg accgcctcga cgaggtcgac
       61 gggattcgag caggtcgcga gcgggagggc agccagcgcg aagactaccg ccgctgcaaa
      121 agccgcggcg agcctagccg ccgcgcgcca ggaaatcatt cgtcccatgc gtccacctcc
      181 cgcgagacgg cctcggaggc gcgggccgtc gtgacggctc ctccctgaag gtcgatcttc
      241 gccgcgagct cggggctcac caccctgagt cgatccaagg ccttgttcat cttctcgtag
      301 tcctggaggg cgtagagggc ccgcgccgcg cccgagaggg agaccgcgct gtcgggggcg
      361 agggccaagg cccgcgcgta ctgctcgtag gcgcccttca tgtcgccctt caggtacagg
      421 gcgttcccca ggttcgtgag ggcgggagcg tagcccccgc tcctggcggc ggagcggaac
      481 tgctcctccg ccttgtcgag caggccgaac tgggcgtaga ggatgccgac gcggttcgcg
      541 gcctgctcgg cgctgcggcc ggaggcccgc tcgctagaga gagcttccgc gacgcgcggg
      601 ccgacggcgg cgtcgaggaa gagccgcatc tccttgcggt agccgtcgac gatgcgctcg
      661 ggcgagggga tcgagaccgc gatgcccccc tccacgaagc cgacaggctc gtagagcttc
      721 caggcctccc ggacggggta gaagccgccc tggcccgccg cctcggcctg gctccactcc
      781 tgggcgccca cgcgccaggc gcggacgaag ccttccttca cgagcgtcac ctcgacgggg
      841 acccaggccc gcccctcgcg gacgacgagg gtctgggggg cgaagaggcg cttggcggcg
      901 ccttcggaga ggccggtgtc gaaggcgacg aagatgtggc cgggggtggt cacgaaggcg
      961 gccgcgacgc ccacggactc gaggagggcg gcgtagagga cggagaggtc gtcgcaatcc
     1021 cccgcccgat aggagagggt ctggttcggg aactggagga agtcgaccgc cgtctccgcg
     1081 gtcgagaggg ccgtgtaggg ggtcttgggg tcgaccgcgt agccgaggcc gtagagcttc
     1141 agggtctgga agagggccag ggccgtcctg aagtcctcgc tcacgggcgg agcgccctcg
     1201 tagcgcaccg tggaggcgac gttcttggcg aagccgagga cgaggggatc cttcgcggtg
     1261 acgaaggccg cggccttgcg gtcgtcgtcc caactcatcg cgttgcggtt ctgcacctgg
     1321 agggtcacgg ggaaggagag cttccgggcc ttgccgaggt agtagtagtc gacgatgacc
     1381 tcgcccgccg ccttggtgcc ctccgtgacg cggaagatcg cgtcattgaa gagggcgtag
     1441 acgggcacct tccgggtctc cccgcccgcg aggctcccga gctcggcgca gagcctgggc
     1501 ccgtccatgt actgcttggc gtagaaggag atgctgacct tctcgtaggg cgaactttcc
     1561 ccgttggaga cgaggacctc tcccgcggga ttcttgtcgt agtaggagta gaagaggggg
     1621 aagatgggct ggaggcgcgg ctccatcctg agctcggtgc ctttcctcgc gccggctagg
     1681 tcgaagccca ggcccagctg gacggagacg ccctcgtaga ggctgcccga aggcgcgagg
     1741 aggcggcggt accgcccgcc gagcccgagg ccgagggtcg ggctcaggcg gagctggacc
     1801 tccgcgccgg cctccaggaa ggggttccgc accgagccca gctccgacat ggccatgtag
     1861 agcccgccgc ccccggcgag ccgcagggag agcctcggga tggggtcgaa ggcgaggccc
     1921 agggaggcgc cgcccgacag gacgcttagg cccccttcgg cgccgttgaa ggggagatag
     1981 tcgaagtcga aggccgcccg gccaaggaga tagggggcga agccggggcg gaattcgccc
     2041 cggagcgtcg cgccgcctcc catgccgaag aggggcaggt cgccgtcgac gacgggcccg
     2101 aggccgatgg agagggatgg caggaccgtg aggccgacgt cgccgacggg agctgatgat
     2161 gccagaaagg gagcagaaag gaggagaagg gggatgagaa gatagaccaa accacgacga
     2221 gccatgggct gcctccggag agggcggacg ttcgtgactt gcgtttaggt atacaatcaa
     2281 tgatagcgct aatcccgagc ttttcaatga tgcttggcgc ggtcggacat gcgatcatgg
     2341 cggcgtgaaa caaaggccgc ggcgagccgc cgggtccggg acatgcgccg gcgcgaccta
     2401 cgacaggttg aggagcatga aggacatgtc gtcttgctgg ggcgtggttc ctcgatgccg
     2461 gtccagggcg gcgggcaggg cctcgcggag ggactcgccg cgggccaggg ccgcgagggc
     2521 gcaggcggcg aggcgcctct cgccgaattc ctcgcccgcg ggattctcct gttcggaaag
     2581 gccgtcgctg tagacgagga gggcgtcccc ggacaggagg ctccagcgag cgatggcggg
     2641 cctgagctcc ggctcgacgc cgatggggag gttccgctcc ggggccttga gccgccttgc
     2701 cttgccctcc cggaggacga gggcgtggga atggcccatg tcggcggcct cgagcgcgcc
     2761 agaacggggg tcgaagacgc agaagaagcc ggtcaggtac ttctcgaggt ggaaggttgc
     2821 gaccagggcc tcgttgaggg cgacgaggag ggaagcgagg cccttcccgt agtcgtgcat
     2881 cctgagcatg ccccagacga gggagacgac gagggaggcg gcgacgccct tcccggagac
     2941 gtcgcagagg gcgacgaagg ccgaaccgtc ctcgaggcgc ttcgagaacc agaaatcgcc
     3001 tccgacgccc ttggaggggc gggaccaggc ctcgaagcgg tacccctcgc cgccgacggc
     3061 ctcgttgccc tcctcgagcc gctcctggat cctgcccgcg agttcgatgt cgtcctccgt
     3121 gatccgcgag aggtgctccg cgaggtcgcg ggaggagagg atggcccgga accggccctc
     3181 ggcgtcgacg aggatccggt acggggcttc ccccccggcc cgcgcgccgg cggagaagag
     3241 gcccgagtgc gcgtcgagaa gcggcgcgcg ctcggcgatc tcccaggccc tcgtgcggcc
     3301 caggacctcg cggccgaagg gcttcccgag gagggcgaag aggccctcgc gggagacgag
     3361 gccctcgggg cggccctccg cgtcgaggag ggcgagcacc tcgatctcgg gacgccgccc
     3421 gagctcgtcg gcgagggccg tcgcgccggc ctcgcggggc gcgcaatgcc ggtggcgcgc
     3481 cgcgcggagc accgtggtgc cgtagtcgcg ggccgcctcg gcctcggtga tcaggaatgc
     3541 gtgtcgctcg cttgcgctca tgggggccac tatcctccct caaggttagc gccgggttag
     3601 gtccgcgtga tttatcgata atctagccgc ctcctcacgg aagcctcatc gcggccgccc
     3661 atcctgcccg cgagcgcagc ggaaggagga cagcatggaa tggacgccgg tcgcgaccga
     3721 atcgggggac ggggcggaga tccgcctggt cggggatttc gacctctacc acgcccccct
     3781 cttcagcctc ttcgtcctcg ggaagatcga gggcggatgg aggacagtcc tcctcgacct
     3841 ctcgggggtc ggctacctcg actccacggg ggtcggctcc atcatcagga tagcgcaggc
     3901 cctgcgcaag aaaggcacga agctccgctt ccggggcctg tcgggaacgc cgcgcagggt
     3961 gctcgagatg gcgaacatcc tggcgctcgt ggaagagacc aagtagcgaa ggagggccga
     4021 tgaaaggcct cattgtcagg aagctcatcg tcgacgagag gaacgagctc ttcgacgcgc
     4081 agggcatgcg cctcgtcgag ttcccgagcg acttcggccg cgtcagggaa tacgccgcga
     4141 aggtcctgga ggactgcccg gagtccttcc gggaggggaa cctcctcgag cagcagctct
     4201 cggagatcat caagaacggg atcaagcacg ggaaccgccg cgacccctcg aagcggctcc
     4261 gcgtctggta cgacctccgc aagcgggtcc gcttcatcgt cgaggacgag ggggagggct
     4321 tcgccgagct cgacgcctgg aacgagttct accgcaagag gcaggaggcc ctcttcgccg
     4381 aggatttcga cgccttcctc gcgatggcga gctaccgggg cccgaggagc gacgagaccg
     4441 acggcggcaa cagcctggtc gcggccctcg agtactggaa cggagggatg atctacaacg
     4501 ccgcgaagaa caaggtcggc gtggtgcgct ggttttccgg gggctcggac tagggggcgc
     4561 ggcggcgcgg cgaggcgcgg ttttaaccga atcctcacgc cttcctcacg ccgcgtccat
     4621 cgcgatccgc ttcactgtca tcatgaagtt caaggagttc ctcgcgtcct actcggcctt
     4681 cctcgcgtcc tccccccggg aggagctcaa ggagggctcg cgctacgtct tcctttcgga
     4741 cctccacctg ggcgacggcg gctcgcgcga cgacctcgcg cccaaccgcg gcctcgtcca
     4801 gaacatgctc tcgaggtact acctggagcg cggctacacc ctcgtcctca acggggacat
     4861 cgaggacctc tcgaagttcc gctacactga catcagggcg gcctggtcct ccctctacgc
     4921 ggtcttcgac gccttcgcga aggagggcag gctcaggaag ctggtcggca accacgacct
     4981 gggcctcctc aggatcaagg accatcccta cgagctcagc cacggcctcg tcctggagcg
     5041 cggccgcgac cggctcttcg tcttccacgg ccaccaggcc tccaagttct tcacgcggca
     5101 cgactacctc tccgacttca tcgtgcgcta cctcgcgaag cccctcaaga tcaggaacac
     5161 gagcgtggac gacgactcga agcggcgctt caagacggag cggcggatct accgcgcctc
     5221 gagggccctg ggcgtcgtag ccgtcacggg ccatacgcac cgccccctct tcgagtccct
     5281 ctcgaagtac gacagcctcc gctgggccat cgaggagctc gtcgcggaat accccctcgc
     5341 ctcggggccg aggcgggagg ccgtcgccga gatggtggag ctctaccgcg acgagctctc
     5401 gaggctcggc aagcgggagc tcgccttcga cctctcccgc agcctctacg accgcggctc
     5461 cctcctcgtg ccctgcctct tcaactccgg ctgcgcgacg ggcaagcacg gcgccacggc
     5521 gatcgagtac gcgggcgggg agctcgccct cgtccactgg acgggcaaga aggggccccg
     5581 gccctacgtg gagcgggagg ccctcatgaa ggaccggatc gagggcacgc cctacgcgag
     5641 gtacatcctc cggagctccc cggcctccca cgtcttcgcc cgcgtcgagc tcctgggccg
     5701 gggcaaggcc gggaagtgac ggccctggac ccctcctgga ggctcgccct ctccaggctc
     5761 gaccacgcgg tctgcgggcg ggagggcgaa aggcctcccg cccgccgagc cattatggcc
     5821 gcggcggggc cggactcgtg gacaagtcgg ggcggcctgg cgtagcatcg agggcgaccg
     5881 gcgaggagga cagcatgggc agagtcttgg tcacctacta cagcgccagc ggcggcacgc
     5941 gcggggccgc ggaggccatc gcctccgtgc tcagcgagcg cggggcggcg gtggattgcg
     6001 cgacgctcgg cgaggcgagg gacgtcgaag gctacgacgc ctttgtcatc ggcgcgccgg
     6061 tcaacggctt ccggtggaga ccggaggccc tggccttcgt ggagctgcac cgggccgtcc
     6121 tggccggaaa gcccacgggc ctcttcctcc tctcgatcat gtacggcctc gggcggccga
     6181 gcgcccgccg gcgcgccctc tccttcctcg atcccgccct ggccctcctg ccccgcgcga
     6241 aggcgggcta cttcggcggg acgatgcagg ggccggcgcc ctggcccctc cgcctggcct
     6301 tcggcgtcaa gaaggacgcg cccctggact cgcgggactg gcccgccatc aaggcctggg
     6361 cggcggagtt cgcgaaggag cttgcgtgag gacgccgccg gcaggctgac ggagacagac
     6421 gcaggccttc cgccggccct aggcgaccgc gacccgcgcg gcgacctcca cctcgacgtt
     6481 gcccgcgatc gcgagccaca cgggcgagag gctcttcgcg gtcttcacga aggccgcgag
     6541 ctcggcctcg gaggtgtcgg gggagtcgag ctcgacctcg acgcggatct tcgcgaggga
     6601 gaggggcttc tcccgcttct cgcccgcggc gcgcatcgag taggactcga cacgcttgcc
     6661 cgacttcctg aggaggaaga cgatgcaggt gctcacgcag ccgccgaagg cgaggaggag
     6721 gagctcgagg ccccggaagc cctcgccgtc gcccgcgggc ggaagataat cgatctggac
     6781 gggcctcccc gggttggtgg cggagacccc ctcgaagagg accttctggt tcttgagcgt
     6841 gacgactgtc tcgagcgcgc ttgcgtccat ctgcttctcc ttgcgcgaga aatgtgcgga
     6901 ccccggaacc gggcgtccta ggaggcagta tcctccctcg cgcgggaaac ggcttgtacg
     6961 tttcggacat tcgaagggag ctcgccgcag atttcgcgga attccctgag gaagtggggc
     7021 tggtcgtagt agcccgcctc ctgggcggcc cggctgcccg cgagctcggg gtggcgcaag
     7081 acgaggtcga gggcgcgttg gaagcgcacg atccgcgaga aggccttggg cgggaggccc
     7141 tggccctcga ggaagaggcg ggagaactgc ctgacggaga ggcgggcgtc ctccgcgagg
     7201 agggcgacgg gcttcgtccc cccgtggaga tagatgtttc cgagcgcctt ggcaaagcgc
     7261 tcgtccgcgt cgcggaggcc cctccccgcg cgggcgagga gccaggcctc ggcgaccgcg
     7321 acgcgggccg ccgcggagcg ggcctccgcg agccggccct cgagctcggc gaccgcgttc
     7381 ccgccgagct ccgagaagga aggggagcgg tcctggaact cggaaagcgg gaggcggaag
     7441 aagcgcccga tcgcgcccgg ggcgaagcgg atgccgaaga agtccgagcc gggcagcatc
     7501 ggcgccgcga tggcccgggt cgcgatgccg aggagcttga ggcgctggaa gcgggagagg
     7561 tcgaagtaga ggtcggcgca gaggtcgggg acggcgcggt aggagaagga gaggggccgc
     7621 cggggcgagg agaagctcca gtagcagtag acgagtccct ccagggcctg acagggaggc
     7681 tgctcccggt actcgatccc ctggccggcg caggccatcc cgggttgcct aggccgatag
     7741 agccccttca cgacgctatg gtcgtcctcg gcgccgctcc tgtcaaccga ttgcgagagt
     7801 cgccgaccgc cccgggacgg cgcggaagac gcgcgacggt ctggccaagc ccggacctcc
     7861 ctcatttaac cgtttcctaa catacatcga atacgggcct catcggccgc gcgtatggtt
     7921 cacgcatccc gacagcggga atctcgaagt tcggaggatg ccatgaagaa gacgattctc
     7981 gtcgcggctc tcgccctcgc ggccgtcgcc tcgttcgccc agggcaaatt cgcctggatc
     8041 gatcccgttt cggacgacaa ggacaagatc cttcccggcg tcgatcccac caaggtggcc
     8101 ggcaccatcg tgaccgccgg ctcttcgacg gtcttccccc tctccgaggc cctcgccgag
     8161 cgcttcaaga aggaaggctt catgggccag gtcaccatcg actccatcgg caccggcgcc
     8221 ggcttcgagc gcttcgccaa gaccggcgag accgacatcg ccaacgcctc ccgcgcgatc
     8281 aagtcctccg aggccgactg ggccaaggcc ctcgcctcgc ccaggacccc gatcgccttc
     8341 caggtcggca ccgacgccct ggccgtctgc gtgagcgcca agaacaagtt cgtccaggac
     8401 gccacggtca aggagctcgc cctcatcttc tccaccgcgc agaactggtc cgacgtccgg
     8461 cccacctggc ccaagaagga gatcaagcgc ttctcccctg gcaccgactc cggcaccttc
     8521 gactacttcg tcgagcacgt cttcgcgaag aagaaggacg gcatcctcgc cgccaagaac
     8581 ctccagctct ccgaggacga caacgtcctg gtccagggca tcgccggcga cgagtacgcg
     8641 atcggctact tcggcttcgc ctacgccgtg gagaacaagg gcaagatccg ccccatctcc
     8701 gtggacggcg tcgagcccaa ccaggccaac gtggacgcga acaagtaccc cctctcccgc
     8761 cccctcttcc tctactccga cgcccgcatc atcgcggaga agccccaggt cgcggccttc
     8821 ctgacctact acctcaccta ctcgaacgag ctcatcaaga aggtcggcta cttccccgcc
     8881 cccgccgacg ccacgaggaa gtccaagcag gccctcgcgg acgccatgaa gggcaggtac
     8941 taaccagcga tgtccgatac cggcagctac cccgagggga agaccggtac ggcgagcctc
     9001 gcccggcgcg caaggccggg cgaggctctc gtgcagtcct tccttttctt ctgcgcggcg
     9061 gtctccatcc tcacgacggt cggcatcgtc gtcaccctcg gggaggagtc cttttccttc
     9121 ttcaagggcg tgaacctcct cgagttcttc acgggcaagg tctggcagcc cgccatcgag
     9181 cgcttcggca tcctgcccct cctcgccgcg accctcacga cgagcctcta cgcgatgctc
     9241 gtcgccctgc ccctggggct cctcgtcgcg gtctacctct ccgagtacgc gagcgcgcgg
     9301 gtcagggacg tcctcaagcc cgtcctcgag gtcctggcgg gcatcccgac ggtcgtctac
     9361 ggctacttcg ccctcacgac cgtcacgccc ttcctccgct ccctgatcgg cgaagacgtc
     9421 gtcgaggtct acaacaccct ctccgccggc ctcgtgatgg gggtgctcat cctccccctc
     9481 atcgcctcca tgtgcgagga cgccctctcc tccgtccccg ccgccctccg ggaaggcgcc
     9541 tacgcgatgg gcgccaccaa gctcgaggtc tccctgcggg tcgtcgtgcc gggcgcattc
     9601 tccggcctgg cggcggcctt catcctcggc ctctcgcgcg ccgtcgggga gacgatgatc
     9661 gtcgccctgg cagccggcgc gggccccaac ctgaccctga accccttcag gtcggcggag
     9721 acgatgacgg gccacatcgt ccgcatctcg ggcggcgacc tctcctacga ctccgtcgac
     9781 tacaagagtc tcttcgcgat agcgatggtg cttttcctcg tgacgctcgg cctcaacatg
     9841 ctctccaggc gcatcatccg ccgctaccag gaggtctacg aatgagcgcc ccggccaagc
     9901 cgcaggcggc cctgatcccc ggcgagcccg cggacttcag ccccaacctg gacgccagga
     9961 aggcgaaggg cgcgaagtgg cgagtcgcct tcctgggctc gaccatactc tccgtccttt
    10021 tcctcttcgc cctcgtggcg agcatcgtca accaaggctt cggctacgtc ctcaccgtgg
    10081 cggaggtgga gcccgcgagc ctcgaacgcg acggcgtccc gctggagagg caggacgtcg
    10141 ccgccctcgt ggagacgatc cgcgacaacg tgccgcgcag gttcttcatc aaggtcgacg
    10201 cggaggcgcc cctcgcccag cggagccgcg aggagctcct cgccttcgtc gaggctgagg
    10261 tcgtcaaggc ccgcgtcgtg ggcacctggt ccctcgcgga gagcctcctg gccaaggagc
    10321 gcatcctcgc cttcaaggag gccgaggcgc ccgagggccg gctcttcttc cgctcctggg
    10381 tcaaccgcgc cctcctttcg aacccccagt cgagcaccgc gatgctcgcc ggcctccgcg
    10441 gcgcggtcat cggaagcctt ctcaccatcc tcttcacgat cgccctcgcc ctgcccctgg
    10501 gcataggcgc ggccatatgg ctcgaggagt acgcgaagga caaccgcctg aaccgcgtga
    10561 tccgcaccaa catctacaac ctcgcaggcg tcccctcgat catctacggc atgctgggcc
    10621 tcgccctctt cgtccgcgcc ctcgagcccc tcacttcggg gagcatgttc caggccgccg
    10681 gggcggactc aagcggcggc gcgaacgggc ggacgatcct c
//
LOCUS       DAOOSJ010000065        10557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4091_length_10557_cov_5.752714, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000065 DAOOSJ010000000
VERSION     DAOOSJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_10557_cov_5.752714"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLG14_04760"
     CDS             complement(<1..1155)
                     /locus_tag="PLG14_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOX48477.1"
                     /translation="MTEASKETIAIDGHSLDLQKVARVARFGAKAALAPEAEARVAAS
                     RAVVDRCVAENIVRYGITTGFGKFCDVVISKAENAELQKNLIMSHACGVGDHLPEEAV
                     RAMALLRANALAVGYSGIRPSTLKSLIAMLNSGVVPLVPEKGSLGASGDLAPLSHMAL
                     VLCGMGEAYYKGVRMTGAQALRAAGLEPVELSAKEGLALINGTQVMTAVGALAAYDAE
                     RLVRLADLAAALTAQALRGIVDAYDPRVHELRGQPGQVASAAALRAILSGSRMTTRQG
                     ELRVQDAYGLRCVPQIHGASRDAIGYVAGVVGRELNAVTDNPLILPADLSKGFPTEIA
                     EASDFGAANPFDNLDADPGDLGLGTPQFGAVSHDASPSFGSDSLDDDDEGD"
     gene            1338..2237
                     /locus_tag="PLG14_04765"
     CDS             1338..2237
                     /locus_tag="PLG14_04765"
                     /inference="COORDINATES: protein motif:HMM:NF009888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOX48478.1"
                     /translation="MLDYRLVEAFAAVLEERGFERAALRLRITQSAVSQRVKQLEDEL
                     GAVLIVRESPPRATQAGERLLRHYRQVLGLEEEAVGEIGARPESGYQHLPLAVNTDSL
                     AVWLLDAVLPAFRGRKVTFEILVEAQDRTLAFLKSGAVVACVSPQREGIQGCSSTLIG
                     RMRYVLAAAPGFRDRWFPGGFGRAAVEEAPLIHYSREDPLQHRALALVFGEPRPEPPA
                     HYLPSTEKYFEAIAAGLGYGLVPEVLAAPALASGALVELEPRARIEVPLYWHRWNRHS
                     ALLQELTEAVLAEGARILEGGPA"
     gene            2359..3417
                     /locus_tag="PLG14_04770"
     CDS             2359..3417
                     /locus_tag="PLG14_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOX48479.1"
                     /translation="MPDATRNDRPESDSPQAEPARAGGPGCPLPGASPRETALATAAL
                     PATALPAAALPAAEPKAFLSPPEIAAAFVESGARKAGLGAGKLLLLGLLAGAFIAFAS
                     EGSSMAAFNLLARPETYGLGRALAGAIFGTGLMLVVVAGGELFTGNVLILLSVLERRT
                     RLAAMLRNWLLVYAGNLAGSLLVACMMARSGLFDSGASVLGGMTIKIASRKTALHFEE
                     AFYLGVMCNWLVCLAVWMATAAKDVAGKLLAVFFPIWLFITSGFEHSVANMYYVPAGI
                     LAKANASWAGAALALGVGPEKLAALDWGSFAFRNLLPVTLGNIAGGGLFVAFAYWLAY
                     LRGGGNSGKGGEGGAARP"
     gene            complement(3532..4113)
                     /locus_tag="PLG14_04775"
     CDS             complement(3532..4113)
                     /locus_tag="PLG14_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48480.1"
                     /translation="MSRKELEAALDYIMNRADEEEFEVILKACERRRRDRSLLGKIGG
                     LGPSESARRAADRIQGQMGYSLDGIRSMVKDFVADIIRKNAPEIPEEDLSALLDAYVP
                     DPETARGRPGEGSRTALPPEALLKMAEQFVEYSGGTMSPSRQRALWEEMPRWQDAYWQ
                     AFTPEMKILIKAYLEGKLDAEEYAKAIVSILEL"
     gene            complement(4139..4633)
                     /locus_tag="PLG14_04780"
     CDS             complement(4139..4633)
                     /locus_tag="PLG14_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOX48481.1"
                     /translation="MANYHLRRADREMTDQDEIKGMLREGKYATVAMDGGDGPYLVVL
                     SYGYDEERNALYFHSALKGHKLDCLARSGKVCALVMQDLGYMKTMCEHAYKSLVLRGE
                     MSVVEDLEEKKRGLDVLLRHLEEDPAPILKRNIKDDSSYAKVAILKLAIGSITAKRCL
                     RPED"
     gene            4884..5771
                     /locus_tag="PLG14_04785"
     CDS             4884..5771
                     /locus_tag="PLG14_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX48482.1"
                     /translation="MKRDRATATAMGLATALIWGLTFLSIKVVVAVFPPMSLGFARFV
                     VAVAILPLVALATKESLRVAPRDLPLLAASGLVGVTLYFLCENNGIMLLSASEASLII
                     GSIPVLTMLAERLFLGSSLGGRAYLGAVLSLAGVGLIAARSPGAASSPMGFVFMAGAA
                     ISWVGYSFLTRPLGSRYGRVAITFWQSLFGMLGFVPFVLAELPAWAMPGPVVVLNILY
                     LGVFGSAIGYWFYVTSLDILGAGAASAFINLIPVVSVVAAYLVLGERLAGLQLLGGAV
                     AVLGVWLATAPAKEKAKAS"
     assembly_gap    6254..6353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6354..7604)
                     /locus_tag="PLG14_04790"
     CDS             complement(<6354..7604)
                     /locus_tag="PLG14_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48483.1"
                     /translation="MRKPLSPLRPLCLFALLACLATGARGLAAEELWSESGFYLDLPE
                     GYALSGGDGKTRFAFVDPAGGMEFDILLYEQGRFKGAEALAAESLGKLGSKGERESFA
                     YEGREAVFAELAFSLGGAAKRGYAVFIGGRPPARGSAGNPGGAGNPGGAGNPGGAGPG
                     ALAGPAAKPAAEPHVALLAYSDEARFEAYADLVLSCLDSFSADRAARRAPGPLSQYLL
                     PASAPTEKKILVFGGASLELPWDQAGADQCLSTAGREFRVLEAYGGTEDLWKAAWARC
                     YRMIYRESARRLDRVAAELDRLLPDAPTDAARKLLEWVQDFRYERDQAGIDFVDPLTA
                     AFEGRGDCDSRAMVMAIILERRGVDALLMLSRDYSHAMAAVDVPGGGQRFPFEGRDWL
                     VAETTAKVGLGMIAADQADWKKWLG"
     gene            7935..8915
                     /locus_tag="PLG14_04795"
     CDS             7935..8915
                     /locus_tag="PLG14_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOX48484.1"
                     /translation="MITIRKNEGTRLIEIDGGEKNCWVDVRDPSREELSRLEAEYRIN
                     PEHVQDILDIDEQARIEKEDEYTLLIVRLPVYDARFEVAYYTAPCGIFLFSDRILTIC
                     QTDCEALEEIRSGRVRGLDVRNKSAFVLHFFGRAAIVYLRYLKDLNRRTAVIERELQR
                     SVKNHELTQLLAFEKSLVFFTTSLKTNEIVLEKLQATKTIRFKEDETELLEDVLTDNK
                     QAIEMANIYSDILSGMMDAFASVISNNLNMQMKRLTIISIVLMIPTFIVSMFGMNVKV
                     PFEDTPHAFALISVACAAAALLGAFVLRDRKPRRPSRLSGPAAVGVVAKR"
     gene            complement(8967..9884)
                     /locus_tag="PLG14_04800"
     CDS             complement(8967..9884)
                     /locus_tag="PLG14_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOX48485.1"
                     /translation="MALLKRASVIALVGNAALAGLKLLTGWLGGSLAVLGDGIDSSTD
                     VVISVMTLVTAGIVGKPGDKEHPYGHARAETIATTILAFIVFFAGGQLLFGAVSGIVS
                     GVEPELPSRLALYATGISVLGKIGLAWSQEHYGRKSGSAMLIANAKNMRGDVVTSLSV
                     LVGLGLTFVLGVPLLDRVAAVVVALWILKNAVGIFRDANTELMDGSEDHGPYASVFEA
                     VASVGGAVNPHRTRIRRLGAELMVDLDIEVDPEMSVAAAHRVAQEVEAAIKARLPEVY
                     DVIVHVEPRGNREEGEKFGLKPEGREKGE"
     gene            complement(10130..10543)
                     /locus_tag="PLG14_04805"
     CDS             complement(10130..10543)
                     /locus_tag="PLG14_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48486.1"
                     /translation="MDKRIAICAHGPAKASAVALLLAAGLALSACSPLKQSAIQGSLY
                     GEKSATTSLELAASDARLSVSGRVELEAGGASVELFEPSGALAWRSEYLAGEGLSSFP
                     VGPYERRGLSGTWILSVKGTAAEARGGYDLMLTNQ"
BASE COUNT         1441 a         3728 c         3629 g         1659 t
ORIGIN      
        1 gtctccctcg tcgtcgtcgt ccaacgaatc cgatccgaac gagggggagg cgtcgtgtga
       61 cacggcgccg aactgaggag tcccgagacc gaggtcgccg gggtcggcgt cgaggttatc
      121 gaacgggttg gcggcgccga agtcggaggc ttcggcgatc tccgtgggga atcccttgga
      181 gaggtcggcg gggaggatga gggggttgtc ggtgacggcg ttgagctcgc ggccgacgac
      241 gcccgcgacg tagccgatgg cgtcgcggct cgcgccgtgg atctggggca cgcagcggag
      301 gccgtaggcg tcctggacgc ggagctcgcc ctggcgggtg gtcatcctgg agcctgagag
      361 gatcgcgcgg agggcggccg cggaggcgac ctggccgggc tggccgcgga gctcgtggac
      421 gcgggggtcg taggcgtcga cgatgccgcg gagggcctgg gcggtgaggg ccgcggccag
      481 gtcggccagg cggacgaggc gctccgcgtc gtaggccgcg agggcgccga cggcggtcat
      541 cacctgggtg ccgttgatga gggcgaggcc ttccttcgcg gagagctcta cgggctcgag
      601 gcccgcggcc cggagggcct gggctcccgt catccggacg cccttgtagt atgcctcgcc
      661 catgccgcag aggacgaggg ccatgtgcga gaggggcgcg aggtcgccgg aggcgccgag
      721 tgagcccttc tcggggacga ggggcacgac gcctgagttg agcatcgcga tgagcgattt
      781 cagggtggag ggcctgatgc ccgagtagcc gaccgcgagg gcgttcgcgc ggaggagggc
      841 catcgcgcgg acggcctcct ccgggaggtg gtcgccgacg ccgcaggcgt ggctcatgat
      901 gaggttcttc tggagctcgg cgttctccgc cttggagatg acgacgtcgc agaacttgcc
      961 gaagcccgtc gtgatgccgt agcggacgat gttctccgcc acgcagcggt cgacgaccgc
     1021 gcgggaggcg gccacgcggg cctccgcctc cggggccagg gcggccttcg cgccgaaacg
     1081 ggcgacgcgc gccaccttct ggaggtcgag ggaatggccg tcgatggcga tggtttcctt
     1141 gcttgcttca gtcatgggct tgtaagtcct ttcccgcgcg gaaggcgggc tcgagcgagg
     1201 gggcggcggg gcgcgggcgc ccgcgaaggg actgtccgcc cctaagggcc tgggccctcc
     1261 ccatgactgt agtcctaaat cgcctttcag taaaattaat agtttcgata agccattagt
     1321 ggtgctaata tcaagccatg ctcgattacc gtctcgtgga ggccttcgcc gccgtgctcg
     1381 aggagcgcgg cttcgagcgc gcggccctaa ggctccgcat cacccagtcg gccgtctccc
     1441 aacgcgtcaa gcagctcgag gacgagctcg gggcggtcct catcgtccgc gagtcgccgc
     1501 cccgcgccac ccaggcgggc gagcgcctct tacgccacta ccgccaggtc ctcggcctcg
     1561 aggaggaggc ggtcggcgag atcggcgcgc ggcccgagtc cgggtaccag cacctgcccc
     1621 tcgccgtcaa cacggacagc ctcgccgtct ggctcctcga cgccgtcctc cccgccttcc
     1681 ggggtcgcaa ggtcaccttc gagatcctcg tcgaggcgca ggaccgcacc ctcgccttcc
     1741 tcaagtcggg ggccgtcgtc gcctgcgtga gcccccagcg cgagggcatc cagggctgct
     1801 cctccaccct catcgggagg atgcgctacg tcctcgccgc cgcgcccggc ttccgcgacc
     1861 gctggttccc gggcggcttc ggccgcgccg ccgtggagga ggctcccctc atccactact
     1921 cgcgcgagga cccgctccag caccgcgccc tcgcgctggt cttcggcgag ccgcgcccgg
     1981 agccgcccgc gcactacctc ccctcgacgg agaagtactt cgaggcgatc gccgccggcc
     2041 tcggctacgg cctcgtgccc gaggtcctcg ccgctcccgc cctggcctcg ggcgccctcg
     2101 tcgagctcga gccgcgcgcg cggatcgagg tgcccctcta ctggcaccgc tggaaccgcc
     2161 actcggccct cctccaggag ctcaccgagg ctgtcctggc cgaaggcgcg cggatcctag
     2221 agggaggtcc agcctagctt gctccgaagg gcccggggcg aggcgcctgg gcggcatggc
     2281 gccgagcggc gctggtcggc ccggcgggct ccttgccggc gtggcggggc gccgctacca
     2341 ttgtgggagg aggaagccat gcccgacgcg accaggaatg accgccccga gtcagatagc
     2401 ccgcaagcgg agccggcgcg cgcgggcggg cccgggtgcc cgctgcccgg ggcctcgccg
     2461 cgagagacgg cgctggccac ggctgcgctc cctgcgacgg cgctccctgc ggcggcgctc
     2521 ccagcggctg agccgaaggc cttcctaagc ccgcccgaga tcgcggcggc ttttgtcgag
     2581 tcgggcgccc gcaaggcggg cctcggagcc gggaagctcc tcctcctcgg cctcctcgcc
     2641 ggcgccttca tcgccttcgc ctccgagggc tcgagcatgg ccgccttcaa cctcctcgcc
     2701 cggcccgaga cctacggcct cggccgcgcc ctggcaggcg cgatcttcgg caccggactc
     2761 atgctcgtcg tcgtcgcggg cggcgagctc ttcaccggca acgtcctcat cctcctgtcc
     2821 gtcctcgagc ggaggacgag gctcgccgcg atgctgcgca actggctcct cgtctacgcg
     2881 ggcaacctgg cgggctccct cctcgtcgcc tgcatgatgg cgcgctccgg gctcttcgac
     2941 tcgggcgcct cggtcctggg cggcatgacg atcaagatcg cgagccgcaa gacggccctg
     3001 cacttcgaag aggccttcta cctcggcgtg atgtgcaact ggctcgtgtg cctggccgtc
     3061 tggatggcga ccgcggccaa ggacgtcgcg gggaagctcc tcgccgtctt cttcccgatc
     3121 tggctcttca tcacctcggg cttcgagcac agcgtcgcga acatgtacta cgtgcccgcc
     3181 ggcatcctcg cgaaggcgaa cgcgtcctgg gccggggccg ccttggccct cggcgtcggg
     3241 ccagagaagc tcgcggccct ggactggggt tctttcgcct tccggaacct cctcccggtg
     3301 acgctcggga acatcgcggg cggcggcctc ttcgtcgcct tcgcgtactg gctcgcctac
     3361 ctgaggggcg gcgggaatag cgggaagggc ggggagggcg gagccgcccg tccctgagcg
     3421 acgcggcggc cggggcgggc ggcgcacccg gcgcggcgcc gcttcccggc gagtcgtcgc
     3481 gttacacggc gccgacggct atgcgggcca cgcgccggtc cgggcgcgcg gctagagctc
     3541 caggatcgag acgatggcct tcgcgtactc ctcggcgtcg agcttgccct ccaggtaggc
     3601 cttgatgagg atcttcatct ccggcgtgaa ggcctgccag taggcgtcct gccagcgggg
     3661 catctcctcc cagagggcgc gctgcctgct cggcgacatg gtgccgcccg agtactcgac
     3721 gaactgctcg gccatcttga ggagggcctc cggggggagg gcggtgcggg agccctcgcc
     3781 cgggcgcccg cgggccgtct ccggatcggg gacgtaggcg tcgaggaggg cggagagatc
     3841 ctcctccggg atctcggggg cgttcttccg gatgatgtcg gcgacgaagt ccttcaccat
     3901 cgagcggatg ccgtccagcg agtagcccat ctggccctgg atccggtccg cggccctgcg
     3961 ggccgattcc gaggggccga ggccgcctat cttcccgagg agggagcggt cccgcctgcg
     4021 gcgctcgcag gccttgagga tcacctcgaa ctcctcctcg tcggcccggt tcatgatgta
     4081 gtcaagggcc gcttcgagtt ccttgcgcga catccgttct ccttgcggct ccgcgatcct
     4141 agtcctccgg cctgaggcac cgcttcgcgg tgatcgagcc gatcgccagc ttcaggatcg
     4201 ccaccttcgc gtaggaggag tcgtccttga tgttccgctt gagtatcggc gcggggtcct
     4261 cctccaggtg gcgcagaagg acgtcgagcc cccgtttctt ctcctcgaga tcctcgacga
     4321 cggacatctc tccccggagc accagcgact tgtaggcgtg ttcgcacatc gtcttcatgt
     4381 agcccaggtc ctgcatgacc agggcgcaga ccttcccgct ccgcgcgagg cagtcgagct
     4441 tgtggccctt cagggccgaa tggaaataga gggcgttccg ctcctcgtca tagccgtagc
     4501 tcaggaccac gaggtagggc ccgtcgccgc cgtccatggc gacggtcgcg tacttgcctt
     4561 ccctgagcat tcccttgatc tcgtcctggt ccgtcatctc ccgatccgcc cgtcgcaggt
     4621 ggtagttcgc catgagcctg tcctccgtcc cgtgtccgtc gtccgcgcgc ggacggcgag
     4681 tccgccgggg ctccattatc ccgggcccgg gctcgcgctg taaagggccg ggcccggctc
     4741 cggcgcggcg ccgaggaggt cggcgggcgg cagcgcccgg ctccgcctcg gcggtcgcga
     4801 ttcctgcggc gattcctgcg ggctgccacg ctgtcgcggc gctctcctag accaggaaag
     4861 gccgggcagg ctaccatccg cccatgaaac gagatcgagc aaccgcgacg gccatggggc
     4921 tcgcgacggc cctcatctgg gggctcacct tcctcagcat caaggtcgtc gtcgccgtct
     4981 tcccgcccat gagcctcggc ttcgcgcgct tcgtcgtcgc ggtggccata ctccccctcg
     5041 tcgccctcgc cacgaaggag agcctccgcg tcgcgccccg ggaccttccc ctcctggccg
     5101 cgagcggact ggtcggcgtg accctctact tcctctgcga gaacaacgga atcatgctgc
     5161 tctccgcctc cgaggcctcg ctcatcatcg gctcgatccc cgtcctcacg atgctggccg
     5221 agcgcctctt cctgggctcg agtctcgggg gccgcgccta cctgggcgcc gtcctttccc
     5281 tcgcgggcgt cggactcatc gcggcccgct cgccgggcgc cgcttcctcg cccatgggct
     5341 tcgtgttcat ggccggcgcg gcgatctcct gggtcggcta ctccttcctc acgaggcccc
     5401 tcggatcccg ctacggaagg gtcgcgatca ctttctggca gagcctcttc ggcatgctcg
     5461 gcttcgtgcc cttcgtgctg gccgagcttc ccgcctgggc catgcccggc cccgtcgtcg
     5521 tcctcaacat cctctatctc ggcgtcttcg gctcggcgat aggctactgg ttctacgtga
     5581 cgagcctgga catcctgggc gcgggggcgg cgagcgcctt catcaacctc atccccgtcg
     5641 tctccgtggt cgcggcctac ctggtcctcg gggagcgcct cgcgggcctc cagctcctgg
     5701 gcggcgcggt ggctgtcctc ggcgtctggc tcgccacggc gccggcgaag gagaaggcca
     5761 aggcttcctg aggcgcgaca ccgcgacgcg gcccgctcgg gctttttccc gaagatcgcc
     5821 gaatccgcca ggattcggcg ccgccggagc gcgtaagcgc tccggcgagg gtcctgtcgc
     5881 cgggaaacgc gcgccccttc tccgctgcgc tccgaagggc gcgcgtttcc ccgcgacacc
     5941 cgccgctccg cccggtcctg cgaaaaggct taagcgggtc gtgactctga agggctcacc
     6001 gcggtggcgt ggccctccgc gcgccggcca cgtcgctttt tggaagggga gtcgtgcgcg
     6061 atgagggccg cctcgcggga atagtcgctt gcggcggtgc tttagcgcgc tgccgggttt
     6121 cgggttgacg cgccgcttcg cggcggcgcc tcccctttgc ggcccctagg cgtgccccgc
     6181 ggcgcgaagg cgtccatccg agccgccctt ccttgagcga ggccggacga cgggtgtcgc
     6241 ggcgagacgg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcccagc
     6361 cacttcttcc agtcggcctg gtcggcggct atcatcccga gtcccacctt cgccgtagtc
     6421 tccgctacga gccagtccct gccctcgaag gggaagcgct ggcctccgcc cgggacgtcc
     6481 acggcggcca tcgcgtggga atagtcgcgg gagagcatga ggagggcgtc cacgccgcgg
     6541 cgctccagga tgatcgccat gaccatcgcg cgcgagtcgc agtcgcctcg gccctcgaag
     6601 gccgcggtca ggggatcgac gaagtcgatg cccgcttggt cgcgctcgta gcggaagtcc
     6661 tgcacccatt caaggagctt gcgcgcggcg tcggtcgggg cgtcggggag gagcctgtcc
     6721 agctcggcgg cgacccggtc caggcgccgc gcggactcgc ggtagatcat ccggtagcag
     6781 cgggcccaag ccgccttcca gagatcctcc gtgccgccgt aggcctcgag gacccggaac
     6841 tccctgccgg ccgtggacag gcactggtcg gctcccgcct ggtcccaggg aagctccagg
     6901 ctcgcgccgc cgaagacgag gatcttcttc tccgtggggg ccgaggcggg gaggaggtac
     6961 tggctcaagg ggcccggcgc ccggcgggcg gcgcggtcgg ccgagaagga gtcgaggcag
     7021 gagaggacaa ggtctgcgta ggcctcgaag cgggcctcgt cggaataggc gaggagggcc
     7081 acgtgcggct ccgcggctgg cttggcggcg ggacccgcca gggcccccgg acccgcgccg
     7141 cctggattgc cggcgccgcc gggattgccg gcgccgccgg gattgccggc gctgccccgg
     7201 gcggggggcc gtcccccgat gaagacggcg tagccccgct tggccgcgcc gcccagggag
     7261 aaagcgagct cggcgaagac ggcctcgcgg ccctcatagg cgaagctctc cctctcgccc
     7321 ttggagccga gcttcccgag gctctccgcg gccagggcct ccgcgccctt gaaccgcccc
     7381 tgctcgtaga ggaggatgtc gaactccatc cctcccgctg ggtcgacgaa ggcgaatcgg
     7441 gtcttgccgt cgccgccaga aagggcgtag ccctccggca ggtccaggta gaagccggac
     7501 tcggaccaga gttcctcggc ggcgaggccc cgggccccag tcgccaggca ggcgaggagg
     7561 gcgaagaggc agagcgggcg gaggggagag agcggctttc gcataccaac aagcatatct
     7621 caatcatcgg cccgggtgcg ctccgttcaa ggattttctt ccccttgcct cttatttccg
     7681 cctggcgtat actctgaccg tggagtcgcc ggttcgccgg cgacccgcct tccgcacgga
     7741 aggcgagcct atcgcgacag gaggcaggta tgccggattc cgacggtagt cgtcaggaac
     7801 cgctatcccc gcacatacgc aagccccgtc gcggcaggtc acgcagcgtc tagccggcgc
     7861 gccttcgaca aaggcgcctt ccgccgcctg cggcctccct ccgcccacgg ggctccgagc
     7921 ctaggaggac gcacatgatc accatccgca agaacgaggg gacccggctc atcgagatag
     7981 acgggggcga gaagaactgc tgggtcgacg tccgcgaccc gagcagggag gagctctccc
     8041 gcctcgaggc cgagtaccgc atcaatcccg agcacgtcca ggacatcctg gacatcgacg
     8101 agcaggcgcg catcgagaag gaggacgagt acaccctcct catcgtccgg ctgcccgtct
     8161 acgacgcccg cttcgaggtc gcctactaca ccgcgccctg cggcatcttc ctcttctccg
     8221 accgcatcct gacgatctgc cagacggact gcgaggccct ggaggagatc cgctccggcc
     8281 gggtccgcgg cctcgacgtc aggaacaaga gcgccttcgt gctccacttc ttcggccgcg
     8341 ccgcgatcgt ctacctgcgc tacctcaagg acctgaaccg ccgcaccgcg gtcatcgagc
     8401 gggagctcca gcgctccgtg aagaaccacg agctcaccca gctcctcgcc ttcgagaagt
     8461 ccctggtctt cttcacgacg agcctcaaga cgaacgagat cgtcctcgag aagctccagg
     8521 cgacgaagac gatccgcttc aaggaggacg agaccgagct cctggaggac gtcctcaccg
     8581 acaacaagca ggccatcgag atggcgaaca tctactcgga catcctctcc gggatgatgg
     8641 acgccttcgc ctccgtgatc tccaacaacc tcaacatgca gatgaagcgg ctcacgatca
     8701 tctccatcgt gctgatgatc ccgaccttca tcgtctccat gttcggcatg aacgtgaagg
     8761 tgcccttcga ggacacgccc cacgccttcg ccctgatctc cgtcgcctgc gcggccgccg
     8821 cgctgctggg cgccttcgtc ctccgcgacc gcaagccccg tcggccctcg cggctctcgg
     8881 ggccggccgc cgtcggcgtg gtcgccaagc gctagaaggt ccgcccggcc gcggtcccgc
     8941 gggccggccc ggggcggacc gcgcgcctac tcgcccttct cccgcccttc cggcttgagg
     9001 ccgaacttct cgccttcctc gcggttgccc cgcggctcga cgtggacgat cacgtcgtag
     9061 acctcgggga ggcgggcctt gatcgccgcc tcgacctcct gggccacgcg gtgcgcggcc
     9121 gcgacgctca tctccggatc cacttcgatg tccaggtcga ccatgagctc ggcgcccagg
     9181 cggcggatcc gcgtccggtg cggattgacc gccccgccga cggaggcgac cgcctcgaag
     9241 acggaggcgt agggcccgtg gtcctcgctg ccgtccatga gctcggtgtt ggcgtccctg
     9301 aagatcccga cggcgttctt gaggatccag agggcgacga cgacggcggc gacgcggtcc
     9361 agcaggggca cgccgaggac gaaggtcagg cccaggccga cgaggacgga gagggaggtg
     9421 acgacgtcgc cccgcatgtt cttcgcgttg gcgatgagca tcgcggagcc ggacttcctg
     9481 ccgtagtgct cctggctcca ggccaggccg atcttgccca gcaccgatat ccccgtggcg
     9541 tagagcgcga ggcgggaagg cagctcgggc tcgacgcccg agacgatgcc cgataccgct
     9601 ccgaagagga gctggccgcc ggcgaagaag acgatgaagg cgaggatggt cgtggcgatc
     9661 gtctcggccc gcgcgtggcc gtagggatgc tccttgtcgc cgggcttgcc gacgatgccc
     9721 gccgtcacga gggtcatgac ggagatcacg acgtccgtgg aggagtcgat gccgtcgccc
     9781 aggacggcca ggctgccgcc cagccagccc gtgaggagct tgagcccggc cagcgcggcg
     9841 ttgccgacga gcgcgatgac ggaggccctc ttcaagaggg ccactcggtt ttccatgctg
     9901 agcctccgga tgccgatgaa ggcgcggcgg cctgatggcc gccgcgcaag gccagcctag
     9961 caccctggcc cgcgtctcca caacgaagcc gcgcaaaaag ctagcgccgc ctaactaagc
    10021 ggcgccggcg cgcggcgctt cgccccctgg gccgcccgct cgcggaccgg ccggcgacgg
    10081 gcaggggacg ggccggagac aatccgggat cttccgctgc tggctccggc tactggttgg
    10141 tgagcatgag gtcgtagccg cccctcgcct cggccgcggt ccccttgacg gacaggatcc
    10201 aggtcccgga gaggccgcgc ctctcgtagg ggccgaccgg aaagctcgag aggccctccc
    10261 cggcgaggta ctcgctcctc caggccaagg ctccggaggg ctcgaagagc tcgaccgagg
    10321 ccccgccagc ctcgagctcg accctgcccg agacggagag ccttgcgtcc gaggcggcga
    10381 gctcgaggct ggtcgtggcg cttttttcgc cgtagaggct tccctggatg gcgctctgct
    10441 tgaggggcga gcaggcagag agggcgaggc ccgcggcaag gaggagggcg acggcggagg
    10501 ccttcgcggg gccgtgagcg cagatggcga ttctcttgtc catggaccga atatacc
//
LOCUS       DAOOSJ010000066        10543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4097_length_10543_cov_4.911804, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000066 DAOOSJ010000000
VERSION     DAOOSJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_10543_cov_4.911804"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLG14_04810"
     CDS             <1..199
                     /locus_tag="PLG14_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX48487.1"
                     /translation="TKEDLEETMGDFVPPAYPHEIELQNLVAVLECTSKEMVPRRFQN
                     PDRAKLVKDIRELKALLGERE"
     gene            complement(286..1197)
                     /locus_tag="PLG14_04815"
     CDS             complement(286..1197)
                     /locus_tag="PLG14_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48488.1"
                     /translation="MTSVAKERDFRFFGFGLPARLVLAGGLYLLGAGLDLALPGFGLV
                     GVAVAAAGWLPLALRAATNKPDDQGLEEWRPVTMAEMDRLDDSLRETRKLRRLSRAAS
                     PALVAALAAPLLLFLALFGLAAGRRDLSFAAFYAVLFLVPSLFFGKVRVFLPMDIAMK
                     MPSFREALAEERPEGVAVAPYLRFDKDPAGRDIPEDLRLMLELKRPPADLVGVQLQAA
                     INKGPNGPVPYLYAVVLTRGTAGPSYALAKRLKADGFKVEAGGEGEFGTVVVRQATSG
                     GGYYTSPDDCRRLARLCLAYLAKLAGS"
     gene            complement(1190..2692)
                     /locus_tag="PLG14_04820"
     CDS             complement(1190..2692)
                     /locus_tag="PLG14_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX48489.1"
                     /translation="MRLAALRPGTAGPRAGRPGAGRPDAALKARLERLQSLHRGLAPW
                     MLFRPEDSVLILPPNRVYKLGGSGAQVLAFLGKGGSLADIPGMDADKASDLERFFAEL
                     EAASAGRAVALERVPYDFAFTRLPVLGELALTYRCNERCRFCYAGCGDAPCKGAPAAL
                     PGSALAEGAGRELSTAEAKRIIDLFRDEAKIPFFSFTGGEPLLRPDLEELAAYAISRG
                     LRVNLITNGSLAGPERAASLRAAGFDTAQVSLESPDPGIHDALCGVPGAFARTTAGIR
                     ALKAAGISVQTNSTVTRENRESLLRLPAFLKGLGVERFSMNLFIPSGRGLSESGLFVP
                     YSEIGSLVDEVRRLALQEGLTFFWYSPTPYCLYNPVARGLGNKSCAAADGLLHVNPEG
                     QVLPCSSWPEPLGSLLERPFSEIWFSERAARLKNKTFAPASCSGCPSFVACQGACPLY
                     WSYAGEAELEGRRGARSSAPAEAGNPAFAEAGRPAPASAGSSAKRSMSHD"
     gene            complement(2689..4464)
                     /locus_tag="PLG14_04825"
     CDS             complement(2689..4464)
                     /locus_tag="PLG14_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48490.1"
                     /translation="MKPCPRREGPASARARRSRGLLGLALGAWLLLSPAAAFGLSADL
                     ATVDLTLVLRPDGKGTVFERLEWKASGGQMHGFYYEGVPFEPVWDLERSFADLPDGKR
                     YALEIEALGGGRYDVVVARGKGFSGTAHYTLAYGGDFAASGLLGSTRSGEGKDLVYFD
                     WSPVAWDEALESRAVRVVFPRKVGAERLEDSEKAAIPLLTEKSVNEENRIDWYGTRAE
                     DGTYWLTGLFFQTGVRARAEQRLILYFPADYLDFTQAALQAAGGAGAGGASGGGESLL
                     AEPRTGEEPAYEAPPRVDYLDRNLRRRPLVAALVLAALAALALYLYYLKLLSFRKKRG
                     LLKSIAWAGDSWMPPKLLVGSYQVPGKIAQDLHPVEVALLMELPLPRVAAVMIEGLSR
                     QGLLELVEEDPLRVRMLSAAKAESEYEEAFLAAFDSEGRVLSGLMADFFEGAIKKLQE
                     KVWDCDLEATKEFYRKKLAEAEEAERAAKAEAAAAPAGAARPYRPRDPYYGYWAGYAL
                     VHRDHGRYSRLALPPELNTGYADFMRSAACFSGCFAPSGGTVDACYSACHNACHDACH
                     SACHSACHSACHSACHSACVSGSAH"
     gene            complement(4461..5081)
                     /locus_tag="PLG14_04830"
     CDS             complement(4461..5081)
                     /locus_tag="PLG14_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48491.1"
                     /translation="MTFSEYRRHFFRSAAGVGSLVAGPLAGLLGLSLGLGAVASALLA
                     LGLPALLFALGLATGAGPKAAAREAERSLAAKAKERLEAVSLARTRLAALRLAEPSVA
                     AARDLLVLEAGRYLEAALAGGAYDPAAGAAVEESLALVDAWLKEADESSSERRFGQAD
                     AHPFPEAAARVSAALKDKAAVVARGRDSVQSVPTGPDLLAIEEELK"
     gene            complement(5091..6050)
                     /locus_tag="PLG14_04835"
     CDS             complement(5091..6050)
                     /locus_tag="PLG14_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOX48492.1"
                     /translation="MTLERLSLDRAERLSAPSAYRFSFPEPPGEEAPGPRAGQASRAA
                     SGPAAAAGSPDGRGPRAAAGGSDRRGPATAANGLDGRGPAAYLSLRSEGDMRYVRGGT
                     NPNRERFFAGLGLDPASVLGLELSHSRGVAFPLEGAVRAEAEPCAAGPGQCDGILLRD
                     PAYAASVTVADCMPIWLHDRASGAFGVLHSGWKGTGILERALAALRERYGSGPASVSV
                     ILGPAIGSCCYAVPEERALGFAREFGAEAAPFTEGRWRLDLRAANIALARRLGVGALL
                     SVEACTSCEESLGSFRRQGPEAFTRMAALAAYPPRLAAGRGPS"
     gene            complement(6053..7771)
                     /locus_tag="PLG14_04840"
     CDS             complement(6053..7771)
                     /locus_tag="PLG14_04840"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOX48493.1"
                     /translation="MPERTVFDGLVSELSSSERRELLQRIAAAAKVSQEPLFNAEALP
                     SPSSPAVSRLSELGLVKRLVLFFRRLFSGKSLEELLEADELKEIAHRVDIRAPGLVDY
                     RRGILLEGFLEELKRLRDAARFFYGVLDRSVERDKGAFFAFLGTIELPETNARLLSET
                     DPFAFAESHPEVPEDEIRSALLGAYDEVILSIPEDRRRAMYYDLRSLLFLKRLSGFLF
                     ERLVAAWRPGASPSGGPAASFAEARGLLAELGDILYSLASPPSLELMEALFVFAERDE
                     LGRPRGDVETLVSADLAKAEEALARIRTFNAKVPLGEILRLAAEDPEVSPRALAGGED
                     WLLVYKAFWKERIDARVDEFREERRYRALAEEIASFVGDAGLRTFAFISREERPGVPP
                     VRLELALSFLDGFYRGPFVSSLNRPLKIVLVDGEFYRKENRLEFTDAYDEILRLAESI
                     SALDARLGPDGDLGSSWTQAQFEVAPIAIKHRKLQTVQRNVDEEAERIVKSAGAALLE
                     LVRVIRGILKGEAGGRYDSLANLSYLDGRANKEFQKALSAAKDRCEKAHNLLAQLSGL
                     DLGQTE"
     gene            complement(7798..>8459)
                     /locus_tag="PLG14_04845"
     CDS             complement(7798..>8459)
                     /locus_tag="PLG14_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOX48494.1"
                     /translation="RFAREGGILHRLDYETSGLVLFARSPAALAFLLREQEEGRLLKD
                     YLLVAGAGGGGLSGSRPLLGSPLGGPAWAGRPSELESLLCEARRAGPARVESRFRPYG
                     PGAARVACLAPGEEPGRRKRGSPERSYATLILDGRALEPGELAGLGLPGEAGPSFFEA
                     EARISQGFRHQIRAHFAWLGFPLAGDALYGGQPAPRLCLHARRLEFAHPGTGRPLTIA
                     I"
     assembly_gap    8460..8559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8790..10010
                     /locus_tag="PLG14_04850"
     CDS             8790..10010
                     /locus_tag="PLG14_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOX48495.1"
                     /translation="MKAENRAEPAELAKRAKSWTKNSRRRLALLTYDVRRSSLPAFFG
                     AFAAAVAASALIVFAAERGRGGMFERLFDGVWWAIVTIATVGYGDKYPVTDTGRVFAI
                     LLMVAGIVLTALISGAVASIFVDRRIREGKGLQEIRVKNHLIVCGWNPDAESLLKGLE
                     SQAESASRPLVLANWLEVEAFDALKARFPGLDLRFVRGDFTQESVLKRASAKAARSCL
                     FVPDASGGNSTSNADERTILGCLAFRSLNPEAAVSAEILRPESEQHLRRAAVENVVVN
                     GELAGYILSAGSVSKGLPRAARSLLSAGEGPRLREAVFPPALVGKSFAEASSWFLQNG
                     KGVLVGLLSEEKGVSLHDLLSDDSSAIDAFIRRKFSEAEIDLEAQMKAGSELKLAPGP
                     DYLIREGDSAFVVA"
     gene            10022..10537
                     /locus_tag="PLG14_04855"
     CDS             10022..10537
                     /locus_tag="PLG14_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48496.1"
                     /translation="MSARSDERKRLAEKGFLAGLEGGDIDEVLKAAKRIECEAGRAVM
                     REGDSGDAMYLLAAGEVEVTKNLTLKLGLRDFARADKSMTRITAERAPVFGEMALLAT
                     EPRSATVTAATPCVLYEIESGAFQAICDGNPRLGAELLRRIARILSARVRKGNEEILK
                     LSTALSIALSR"
BASE COUNT         1590 a         3516 c         3996 g         1341 t
ORIGIN      
        1 taccaaggag gaccttgagg agaccatggg agacttcgtg ccgcccgcct atccccacga
       61 gatcgagctg cagaacctcg tggcggtcct ggagtgcacc tccaaggaga tggtgccgcg
      121 gcgcttccag aaccccgacc gcgccaagct cgtgaaggac atccgcgagc tcaaggccct
      181 gctcggggag cgcgagtagg agacggcggg cgcgcggccc cgcgcgcggc aggcggccgg
      241 gagcgcgccg cgggcgcccg gaagctccgc cgcggcggcc cggcttcagc tccccgcgag
      301 cttcgcgagg taggccaggc agagccgcgc caggcggcgg cagtcgtcgg ggctcgtgta
      361 gtagccgccc cccgaggtcg cctgcctcac cacgacggtg ccgaactcgc cctcccctcc
      421 cgcctccacc ttgaagccgt cggccttgag ccgcttcgcg agcgcgtagg aggggcccgc
      481 cgtcccgcgc gtgaggacca cggcgtagag gtagggcacg gggccgttgg gccccttgtt
      541 gatcgccgcc tgcagctgca cgccgaccag gtcggcgggc gggcgcttga gctcgagcat
      601 gaggcgcagg tcctcgggta tgtcgcggcc cgcgggatcc ttgtcgaagc gcaggtaggg
      661 cgcgacggcc accccctccg gcctctcctc cgccagggcc tccctgaagg agggcatctt
      721 catggcgatg tccatcggca ggaagacgcg gacctttccg aagaagaggg agggcacgag
      781 gaagaggacg gcgtagaagg cggcgaagga gaggtcgcgg cgtccggcgg ccaggccgaa
      841 gagggcgagg aagaggagga gcggcgcggc cagtgcggcg accagggccg gcgaggcggc
      901 ccggctgagc cgcctgagct tcctggtctc gcggaggctg tcgtccaggc ggtccatctc
      961 cgccatcgtg acgggccgcc actcctccag cccctggtcg tcgggcttgt tcgtcgcggc
     1021 ccggagcgcc aggggaagcc agcccgcggc cgcgacggcg acgccgacga ggccgaagcc
     1081 gggcagggcc aggtccaggc ccgcccccag caggtagagg ccccccgcga ggacgaggcg
     1141 ggccggcaag ccaaagccga aaaaccggaa atccctttcc ttggcgacgc tagtcatggc
     1201 tcatgctcct tttcgcggag gatccggccg aggccggcgc tggacggccg gcctcggcga
     1261 acgcgggatt cccggcctcg gccggcgcgg agctccgggc gccgcgccgc ccctcgagct
     1321 cggcctctcc cgcgtagctc cagtagaggg ggcaggcgcc ctggcaggcg acgaaggaag
     1381 ggcagccgga gcaggacgcg ggcgcgaagg tcttgttctt gaggcgggcc gccctctcgg
     1441 agaaccagat ctcggagaag ggccgctcga ggaggcttcc gaggggctcg ggccaggacg
     1501 agcagggcag gacctggcct tcaggattga cgtggagaag gccgtcggcc gcggcgcagc
     1561 tcttgttccc gaggcccctc gcgacgggat tgtagaggca atagggcgtc gggctgtacc
     1621 agaagaaggt gaggccttcc tggagggcca ggcgccgcac ctcgtcgacg agactcccta
     1681 tctcggaata ggggacgaag agccccgact cggaaaggcc gcggcccgag ggtatgaaga
     1741 ggttcatgga gaagcgctcg acgccgaggc ccttcaggaa ggcggggagg cgaagcaggc
     1801 tctccctgtt ctcccgggtc acggtggagt tggtctgcac cgagattccg gctgccttga
     1861 gggcgcgtat gccggccgtc gtgcgggcaa aggctcccgg aacgccgcag agcgcgtcgt
     1921 ggatcccggg atcggggctc tccaggctca cctgggccgt gtcgaagccc gcggccctga
     1981 gggaggcggc ccgctccggg cccgcgaggc tgccgttcgt gatcaggttg acgcggaggc
     2041 cgcgggagat ggcgtaggcc gcgagctcct ccaggtcggg cctgaggagg ggctcgccgc
     2101 ccgtgaagga gaagaagggg atcttggcct cgtcgcggaa gaggtcgatg atccgcttcg
     2161 cctccgccgt cgaaagctct cgtcccgcgc cctcagccaa agccgagccc ggaagcgcgg
     2221 ccggagcgcc cttgcagggc gcgtcgccgc agcccgcgta gcagaagcgg cagcgctcgt
     2281 tgcagcggta ggtcagggcc agctcgccca ggacgggaag gcgcgtgaag gcgaaatcgt
     2341 aggggacccg ctctagggcg acggcacgtc cggcgctcgc cgcctctagc tcggcgaaga
     2401 agcgttcgag gtcggaggcc ttgtccgcgt ccatgcccgg gatgtcggcc agggagccgc
     2461 ccttcccgag aaaggcgagg acctgggcgc ccgagccgcc gagcttgtag acgcggttgg
     2521 gcggcaggat gaggaccgag tcctcgggcc tgaagagcat ccagggcgcg aggccgcggt
     2581 ggagggactg aaggcgctcg agccgcgcct tgagcgccgc gtcgggacgc ccggcgccgg
     2641 gacggccggc tcggggaccg gcggtgccgg gccggagggc ggcgagcctc aatgggcgct
     2701 ccccgagacg caggccgagt ggcaggcgga atggcaggcc gagtggcagg cggaatggca
     2761 ggccgagtgg caggcgtcgt ggcaggcgtt gtggcaggcc gagtagcagg cgtcgacggt
     2821 gccgcccgag ggggcgaagc agccggagaa gcaggccgcg ctccgcatga agtcggcgta
     2881 gcccgtgttg agctcgggcg gaagggcgag cctggagtag cggccgtggt cgcggtggac
     2941 cagggcgtag ccggcccagt agccgtagta gggatcgcgc gggcgatagg gcctggccgc
     3001 tccggccggg gccgcggcgg cctccgcctt ggccgcgcgc tcggcctcct cggcctcggc
     3061 gagcttcttg cggtagaact ccttcgtcgc ctccaggtcg cagtcccaga ccttctcctg
     3121 gagcttcttg atcgcgccct cgaagaagtc ggccatgagg cccgagagga cccggccctc
     3181 ggagtcgaag gcggccagga aggcctcctc gtactcgctc tccgccttgg ccgcggagag
     3241 catcctgacg cggaggggat cctcctccac gagctcgagg aggccctggc gcgagaggcc
     3301 ttcgatcatg acggccgcga cgcggggcag gggcagctcc atgaggaggg ccacctccac
     3361 ggggtggagg tcctgcgcga tcttgcccgg gacctggtag gagccgacga ggagcttggg
     3421 cggcatccag gagtcgccgg cccaggcgat cgacttgaga aggccgcgct tcttccggaa
     3481 ggagagcagc ttcagataat agaggtagag agccagggca gccagcgcgg cgaggacgag
     3541 ggcggcgacg agggggcggc ggcgcaggtt gcggtcgagg tagtcgaccc ggggcggcgc
     3601 ctcgtaggcg ggctcctccc ccgtccgcgg ctcggcgagg agactctccc cgccgcctga
     3661 ggctccgcct gcgcccgcgc cgccagccgc ctggagggcg gcctgcgtga agtcgaggta
     3721 gtccgcgggg aagtagagga tgaggcgctg ctcagcgcgg gcccggacgc ccgtctggaa
     3781 gaagaggccg gtgagccaat aggtcccgtc ctccgccctc gtgccgtacc agtcgatgcg
     3841 gttctcctcg ttgacgctct tctcggtgag gagggggatg gcggccttct ccgagtcctc
     3901 gagccgctcc gcgccgacct tgcgcgggaa gacgacccgg acggcgcggc tctccagcgc
     3961 ctcgtcccag gcgacgggac tccagtcgaa gtagacgagg tccttgccct cgccgctccg
     4021 ggtcgagccg aggaggcccg aggccgcgaa gtccccgccg taggcgaggg tgtagtgcgc
     4081 cgtgccggag aagcccttcc cgcgggcgac gacgacgtcg tagcggccgc cgcccagggc
     4141 ctcgatctcc agggcgtacc gcttgccgtc gggcaggtcg gcgaaggagc gctccaggtc
     4201 ccagacgggc tcgaagggca cgccctcgta gtagaagccg tgcatctggc cgcccgaggc
     4261 cttccactcg aggcgctcga agaccgtgcc cttgccgtcg ggccgcaaga ccagggtgag
     4321 gtccaccgtc gcgaggtcgg ctgagaggcc gaaggccgcg gcgggcgaga ggaggagcca
     4381 ggcgccgagc gcgaggccga ggaggcctct ggagcgccgc gcgcgcgcgg aggccgggcc
     4441 ttcgcgcctt ggacagggtt tcatttgagc tcctcctcga tggccaagag atcgggaccg
     4501 gtcgggacgg actggaccga gtccctgccc cgggcgacga cggccgcctt gtccttgagc
     4561 gccgcggaga ccctggcggc ggcctcgggg aagggatggg cgtcggcctg gccgaagcgg
     4621 cgctccgagg agctttcgtc cgcctccttg agccaggcgt cgacgagggc gagggactcc
     4681 tccaccgcgg cgccggccgc gggatcgtag gcgccgcccg cgagggccgc ctccaggtag
     4741 cgccccgcct ccaggaccag gaggtcgcgg gcggcggcta ccgagggctc ggcgagccgc
     4801 aaggccgcga gcctggtcct cgcgagggag acagcctcca gccgctcctt cgccttggcg
     4861 gctaggctcc gctcggcctc gcgcgcggcg gccttggggc cggctcccgt cgcgaggccc
     4921 agggcgaaga ggagggcggg caggcccagg gcgaggaggg ccgaggctac ggcgccgagg
     4981 cccagggaaa ggccgaggag gcccgcgagg gggccggcga cgagactgcc cactcccgcg
     5041 gccgagcgga agaaatgacg gcggtattcg ctgaaggtca tacgggaata ctagctcggc
     5101 ccccggcccg ccgcaagccg cggagggtag gcggcgaggg cggccatgcg cgtgaatgcc
     5161 tcggggccct ggcggcggaa ggagcccagg ctctcctcgc aggaggtgca ggcctccacg
     5221 gagaggaggg ccccgacgcc caggcggcgg gccagggcga tgttggcggc gcggaggtcc
     5281 agcctccatc ggccctccgt gaaaggggcg gcctccgcgc cgaattcgcg ggcgaagccg
     5341 agggcccgct cctccgggac ggcgtagcag caggagccga tggcgggccc gaggatgacc
     5401 gagacggagg cgggcccgga gccgtagcgc tcccgcaagg cggccagggc cctctcgagg
     5461 atgcccgtgc ccttccagcc cgagtggagg acgccgaagg ctcccgaggc ccggtcgtgg
     5521 agccagatgg gcatgcagtc ggcgacggtg acgctggccg cgtaggccgg gtcgcggagg
     5581 aggatgccgt cgcactggcc gggaccggcg gcgcatggct ccgcctccgc ccggacggcc
     5641 ccctccaggg gaaaggcgac gccgcgggaa tggctgagct cgaggccgag gacggaggcc
     5701 ggatcgaggc ccaggccggc gaagaagcgc tcgcggttcg ggttcgtccc cccgcggaca
     5761 tagcgcatgt cgccctcgga gcgcaaggag aggtaggccg ctggcccgcg cccgtccagc
     5821 ccgttcgccg cggttgcggg tccgcgcctg tccgagcctc ccgccgcggc cctcggcccg
     5881 cgcccgtccg ggcttcccgc cgcggccgcg ggccccgagg ccgccctgga agcctggccg
     5941 gcgcggggcc cgggcgcttc ctccccgggg ggctcgggga aagagaagcg ataggcggag
     6001 ggagcggaca ggcgctccgc gcggtccagg gagaggcgct cgagggtcaa ggctactccg
     6061 tctgcccgag gtcgaggccg gagagctggg cgaggaggtt gtgcgccttc tcgcagcggt
     6121 ccttggccgc ggaaagggcc ttctggaatt ccttgttggc ccgcccgtcc aggtaggaga
     6181 ggttggccag ggagtcgtag cggccgcccg cctcgccttt caggatgccg cggatcaccc
     6241 ggacgagctc gaggagggcc gcgccggcgc tcttcacgat ccgctcggcc tcctcgtcga
     6301 cgttgcgctg gaccgtctgc agcttgcggt gcttgatggc tatcggcgcc acctcgaact
     6361 gggcctgggt ccaggaggag cccaggtccc cgtccggccc gagcctcgcg tccagggcgg
     6421 agatcgactc cgcgagacgg aggatctcgt cgtaggcgtc ggtgaactcg aggcggttct
     6481 ccttgcggta gaactcgccg tccacgagga cgatcttgag gggccggttc agggaggaga
     6541 cgaaggggcc gcggtagaag ccgtccagga aggacagggc gagctcgagc ctgacgggcg
     6601 ggacgcccgg cctctcctcg cgggagatga aggcgaaggt cctgaggccg gcgtcgccca
     6661 cgaaggaggc tatctcctcc gcgagggccc ggtagcggcg ctcctcccgg aactcgtcga
     6721 cgcgcgcgtc gatcctctcc ttccagaagg ccttgtagac gaggagccag tcctcgcccc
     6781 cggccagggc ccgcggcgac acctcgggat cctcggcggc gagcctgagg atctcgccca
     6841 gaggaacctt ggcgttgaag gtcctgatgc gcgccagggc ctcctcggcc ttcgcgaggt
     6901 cggcggagac cagggtctcc acgtcgcccc tcggccttcc gagctcgtcg cgctcggcga
     6961 agacgaagag ggcctccatg agctcgagcg agggcggcga ggcgagggaa tagaggatgt
     7021 cgccgagctc ggcgaggagg ccgcgcgcct ccgcgaagga ggcggcgggc ccgccggagg
     7081 gcgaggcgcc gggacgccag gcggcgacga ggcgctcgaa gaggaagccc gacaggcgct
     7141 tcaggaagag gaggctccgg aggtcgtagt acatcgcccg ccgcctgtcc tcggggatgg
     7201 agaggatgac ctcgtcgtag gccccgagga gggcggagcg gatctcgtcc tccggcacct
     7261 cgggatggct ctccgcgaag gcgaaggggt cggtctcgga aaggaggcgg gcgttcgtct
     7321 cgggaagctc gatggtgccc aggaaggcga agaaggcccc cttgtcccgc tcgacgctcc
     7381 ggtccaggac gccgtagaag aagcgcgcgg cgtcgcgcag ccgcttgagc tcctccagga
     7441 agccctcgag gaggattccc cgccggtagt ccacgaggcc gggcgccctg atatcgacgc
     7501 gatgggcgat ctccttgagc tcgtcggcct ccaggagctc ctccaggctc ttgccgctga
     7561 agaggcggcg gaagaacagg acgaggcgct tcacgaggcc gagctcggag aggcgggaca
     7621 cggccggcga ggaggggctc gggagggcct cggcgttgaa gaggggttcc tgggaaacct
     7681 tggcggccgc ggctatccgc tggaggagct ccctgcgctc cgaggaggag agctcggaca
     7741 cgagtccgtc gaagaccgtt ctctcgggca tatacaaaga gagtagatga gcggcggcta
     7801 aattgcaatc gtaagcgggc ggcccgtccc gggatgggcg aattcgaggc gccgggcgtg
     7861 gaggcagagg cggggcgcgg gctggccgcc gtagagggcg tcgcccgcga ggggaaagcc
     7921 cagccaggcg aaatgggccc tgatctggtg ccggaaaccc tggctgatcc gggcctcggc
     7981 ctcgaagaag gagggcccgg cctccccggg gagcccgagg ccggcgagct ccccgggctc
     8041 cagggcgcgg ccgtccagga tcaaggtcgc gtaggatctc tcgggcgagc ctcttttgcg
     8101 tcggcccggc tcctcgccgg gcgcgaggca ggcgacgcgg gccgcgcctg ggccgtaggg
     8161 cctgaagcgg ctctccacgc gcgccgggcc agcgcgtcgg gcttcgcaaa ggagggactc
     8221 aagctcggat ggccggccgg cccaggccgg gccgcccagg ggcgagccca ggaggggcct
     8281 ggagccggaa agcccgccgc ctccggcgcc ggcgacgagg aggtagtcct tgaggagtct
     8341 tccctcctcc tgctccctga ggaggaaggc gagggccgcg gggctcctcg cgaagaggac
     8401 gaggcccgag gtctcgtagt ccaggcggtg gaggatgccg ccctcgcggg cgaagcgggn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaagacccag tccgcgaggc
     8581 agggctcgcc cgaggctccc cgggaggaat ggaggcgggg cggcttgcag gccacgacga
     8641 ggctgtcgtc cgccgcgaca acgaagggct cggccaggct cgcgaaggga ctcggcatgg
     8701 ctacccttat acaatggccg ggggcggcgc ggaaaggccg aaaggccggc gcttccttgg
     8761 ccgatactca aggcggagga aagcgcccga tgaaagcgga aaaccgagcc gagccggccg
     8821 agctcgcgaa gcgcgcgaag tcctggacca agaactcccg gcggcgcctc gcccttctca
     8881 cctacgacgt ccggcgctcg agccttcccg ccttcttcgg agccttcgcg gccgccgtgg
     8941 cggcctcggc cctgatcgtc ttcgccgccg agcgcggccg gggcggcatg ttcgagcggc
     9001 ttttcgacgg ggtctggtgg gccatcgtga cgatcgcgac cgtcggctac ggggacaagt
     9061 accccgtcac ggacacgggc cgggtcttcg cgatcctcct catggtcgcg ggcatcgtcc
     9121 tcaccgccct gatctcgggc gccgtcgcct ccatcttcgt cgacagaagg atcagggagg
     9181 gtaagggctt gcaggaaatc cgcgtgaaga accacctcat cgtctgcggg tggaatcccg
     9241 acgccgagtc gctgctcaag gggctcgagt cccaggcgga atcggcctcc cggcccctcg
     9301 tcctcgccaa ctggctcgag gtcgaggcct tcgacgcgct gaaggcccgc ttcccgggcc
     9361 tggacctgcg cttcgtccgc ggcgacttca cccaggagag cgtcctcaag cgggcctcgg
     9421 ccaaggccgc ccgctcctgt ctcttcgtcc ccgacgcctc gggcggcaac tcgacctcca
     9481 acgcggacga gcgcaccatc ctcggctgcc tggccttccg ctccctcaat ccggaggccg
     9541 ccgtcagcgc ggagatcctc aggcccgaga gcgagcagca cctccgcagg gccgccgtcg
     9601 agaacgtcgt cgtcaacggc gagctcgcgg gctacatcct ctccgccggc tccgtctcca
     9661 agggcctgcc ccgggcggcc cggagcctcc tctcggcggg agaggggccc cggctccggg
     9721 aggcggtctt tcctccggcc ctggtcggaa agagcttcgc ggaggcctcc tcctggttcc
     9781 tccagaacgg caagggcgtc ctcgtgggcc tcctctccga ggagaagggg gtctccctcc
     9841 acgacctcct ctcggacgac tcgagcgcca tcgacgcctt catacgccgg aagttcagcg
     9901 aggcggagat agacctggag gcccagatga aggccggcag cgagctcaag ctcgcgcccg
     9961 gccccgacta cctgatccgc gagggcgact cggccttcgt ggtggcctga gggacgcccc
    10021 ggtgagcgcg cggagcgacg agcggaaaag gctcgcggaa aagggcttcc tggccggcct
    10081 cgagggcggg gacatcgacg aggtcctcaa ggccgcgaag cggatcgaat gcgaggccgg
    10141 cagggccgtc atgcgcgagg gcgacagcgg cgacgccatg tacctcctgg ccgcgggcga
    10201 ggtcgaggtc acgaagaacc tcaccctgaa gctcgggctc agggatttcg cccgggcgga
    10261 caagtccatg acccggatca ccgccgagcg ggcgccggtc ttcggcgaga tggccctcct
    10321 cgcgacggag ccccgcagcg ccaccgtcac ggccgcgacg ccctgcgtcc tctacgagat
    10381 cgagagcgga gccttccagg cgatctgcga cgggaatccc cgcctcggcg ccgagctcct
    10441 caggcgcatc gcccgcatcc tctccgcgag ggtcaggaag ggcaacgagg agatcctcaa
    10501 gctttccacc gccctgagca tagccctttc caggtgagga aga
//
LOCUS       DAOOSJ010000067        10453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4148_length_10453_cov_5.740046, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000067 DAOOSJ010000000
VERSION     DAOOSJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4148_length_10453_cov_5.740046"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1156
                     /gene="ugpC"
                     /locus_tag="PLG14_04860"
     CDS             35..1156
                     /gene="ugpC"
                     /locus_tag="PLG14_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOX48497.1"
                     /translation="MAKVELKKIGKVYEGNVRAVDDANITINDREFVVFVGPSGCGKS
                     TTLRMVAGLEDITEGELLIDGKLVNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKVPKAEIDTRVREAARILDIEKLLDRRPKQLSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAEIIELHDRLDATMIYVTHDQIEAMTMGTKIVVMRDGKVQQIGS
                     PLYMYNHPINKFVAGFIGSPPMNFLTVKVLEEGGKILLDEGSFRIGAAPEHQAALKPY
                     VGKEVSFGIRPEDLLLAEGGSAPHGHIKAKVTVVEPLGAEIQLYASTPTQPMVARIPP
                     HHVYKSGDAIEFAPVMEKARFFDRETELSILPVKFDEQA"
     gene            1293..>1512
                     /locus_tag="PLG14_04865"
     CDS             1293..>1512
                     /locus_tag="PLG14_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48498.1"
                     /translation="MPFNVAERVAVLIGEVGRIESESEDIYLSLGRLFPRLVAEMRKS
                     SETAQASLDGFSSLKEGARGAASRGASAG"
     assembly_gap    1513..1612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1613..3171
                     /locus_tag="PLG14_04870"
     CDS             <1613..3171
                     /locus_tag="PLG14_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48499.1"
                     /translation="AYFRRIHERDSAFLARINESIERLSSLEELIGRVRLDSEEMEII
                     SLNAMTVALKSGNAGKAFSVITDELKRLSSRTISLTEEITARGRALLEYFSRLRDSLG
                     ELDSFQAAFFADLDKALSQGYSELEGDVRSALEVFGGLLADAQSVREPVQNIMQGIQL
                     QDIFRQSLQHVSISLEEARKSAGEASAMGSGSEASAALAEELAFVAAVAELSGSLLEE
                     IVGQLDSAAGEFAKEIEAVSRLIEEVEGRRAAFIGDPERVQEGSVDASRFSAGSGRYL
                     ALKKDVIGTARRLSEQVRVLDESFKGLAGLLARFQNIVVASRIEVAKNRTLAGVTTTV
                     QGMIELTERLGADVGGAMATTKDFIKVASAAIAEYSGGEEAGRRSAREESKAGTAEGD
                     RLQATLRSVEAGIESLDGSRVAVARAVGDFRLYTPEFIALIAEARGALERLRGLSAEL
                     KRAQADLALLKSTVDEEAGPDAPRGAIRSDRLKSMIERFTIFTHKRTAGQIGNFDVEA
                     GVEAGDITLF"
     gene            3184..3774
                     /locus_tag="PLG14_04875"
     CDS             3184..3774
                     /locus_tag="PLG14_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOX48500.1"
                     /translation="MFRHRITHFEQEVVTIHPGEYFATKDETIISTVLGSCIAVALYD
                     PVSQAGGLNHFMLPGEIAPDELVRDPNAKYGMWAMELLINELMKLGVRRRDLKAKVFG
                     GGAVLRFLDGSVSRIPQSNIDFAFEYLEKEGIPILASDVGGREARKVFLFPRTGKVLM
                     KRIGGSLANLVEKEEERYLTTIRTEAPEGEIVLFKD"
     gene            4201..>5144
                     /locus_tag="PLG14_04880"
     CDS             4201..>5144
                     /locus_tag="PLG14_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48501.1"
                     /translation="MDRLLGRARPPAPEVAEPAPLGPLSALEAADPLALAAADYLFPL
                     AFQAGKTPIRGRPTSVRSGEAALEGLPEGPWFWWKAETGAGAAGGYWCLPPSLPPRDA
                     EAAAAWAKTATALAGAAAARLKALLAAAPGLAPSSFAAGAFPQGGAPKLAGKSALRLD
                     FRYAARGAFRRGSAFTSPGYAEILAGALGPGHGRASAGGRLQDLPARSESPAGADPIG
                     ALLSASESILARNPASLIASRSFSFGLPEGPLSYLPLYECLNLLSDADLRLVVQNRLV
                     PKAQGGSFGALFSYREAEPSAPPAGATGTAARPSRPAPG"
     assembly_gap    5145..5244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5245..5746
                     /locus_tag="PLG14_04885"
     CDS             <5245..5746
                     /locus_tag="PLG14_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48502.1"
                     /translation="AVLEEGRLDPEHAAADARDFLARNDAAYEDLFRATRSGSLALSE
                     RGAALARELYLSMVYGPKRRGFEAFLRESRPVQELAGLPERIARRAVDLSGARSLAAA
                     VFGDPGDLAYVAKWCSKGKRAEIEDEQRRVAAALDEGLADLEALKAERGALLAKAKAV
                     MEEERR"
     gene            complement(5956..6861)
                     /locus_tag="PLG14_04890"
     CDS             complement(5956..6861)
                     /locus_tag="PLG14_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOX48503.1"
                     /translation="MEALEAAVEGLLSSYERDGGVNHSGGPNLPSRAAVVLALEDLET
                     LVFPGFRQEDGIDDSNLGLVAGELVHRAAKVLSREAERSLEYRYRLEGKPGCPAACHL
                     EARDLVLGFFEALPALRAILMEDVGAAFKGDPAAKSVAEVILAYPGLEALVVHRIAHH
                     FWKAGLPLIPRMMSESVHGKTGIDIHPGATIGKRFFIDHGTGVVVGETTVIGDDVKLY
                     QGVTLGALSVKKEDGETKRHPTIGDGVTIYSGATILGGCTEIGARSVIGGNVWLTHSV
                     PPDSKVYLPEGEGALVEPRKPASCG"
     gene            complement(6863..8167)
                     /locus_tag="PLG14_04895"
     CDS             complement(6863..8167)
                     /locus_tag="PLG14_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48504.1"
                     /translation="MGGSKKGFDKYLGLTFLIGFGFFTMGLMDILYDTYVPIFLGKYI
                     AKNWIVGGLMTLDNILAVFLIPFVSLWSDNTRTRIGRRMPFIVVLLPLSALCFSLIPY
                     AAFRSLGALLLVIFGLNIFKQSVRGPVVALMPDTIPGDYRSEANGIINTMGALGAIIS
                     TVGLARLMDLDIVLPLLGRTKDALPFPIAGVLVVLATVLVFSFVRERSREEEEKEEKV
                     PLAKALKGIAGEKDKSALWILLALFFWFLGYQGILPFIGKLCKDVFEIASNGKAALPA
                     SAVAIAQTLFAIPSGYVAHRIGRRKAIRGSLLVLVAILGLTAFVASPLAAPLGAALRF
                     NLLLAMMFVYGVFWITIITNSFPMLWQMATFGSIGLYTGLYYTFSQTAAISSPPVTGA
                     IIDFVGYPGIFVFAAFCEFAAFVLMGKVTKGEPGEKPAAAGA"
     gene            complement(8262..>9185)
                     /locus_tag="PLG14_04900"
     CDS             complement(8262..>9185)
                     /locus_tag="PLG14_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOX48505.1"
                     /translation="RGVARGLADRGVAIGGFDAAMDSTVLPGSGLSSSAAVEVLFGTI
                     LADLAGAGLGSAAAREKGIDAVELAKIGQYAENRFFGKPCGLMDQTASAVGGVVAIDF
                     ALPSAPAVRSIPFAFAKAGLVLCVVNTGGSHADLTADYAAVPAEMKAVASFLGKAVLR
                     EVDPALLVERGPAIRAALGDRALLRALHFAAEDARVPAMVAALERNDVAAYLGLVRAS
                     GDSSWRLLQNLYPPEAPHEQGLPLALALSERFLAGEGAVRVHGGGFAGTIQAYVPLAR
                     FEGWKALMESYFGPGSVHPIGARPLGTLRLA"
     assembly_gap    9186..9285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9529..10290
                     /locus_tag="PLG14_04905"
     CDS             9529..10290
                     /locus_tag="PLG14_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX48506.1"
                     /translation="MKQSEKPSRATPLLAALLLGGLAVASAFYFRSYEAQTAPSLLAS
                     DALVRVEKGAYGLAFLPAAGAGSLGLAFYPGARVPPEAYAYLARALAAQGYPTVLLST
                     PLNFAVFAPGKAGAAARAFPEVGAWVLGGHSLGGAMAAARAAKAPGSAAGLLLLAAYP
                     GGGADLSGSGLPVLSVSATNDGLATPAKVQAARSLLPPGARHVVLAGGNHAQFGEYGS
                     QAGDGTAELPAAAQRRAVVQEAAGFLGAVLAAVRP"
BASE COUNT         1505 a         3626 c         3563 g         1459 t
ORIGIN      
        1 caggcaatag cataactccc cacggaggtc tttcatggcg aaggtagaac tcaagaagat
       61 cggcaaggtg tacgagggca atgtccgcgc cgtcgacgac gcgaacatca ccatcaacga
      121 cagggaattc gtcgtcttcg tcggcccctc gggctgcggc aagtcgacga cgctccgcat
      181 ggtcgccggc ctcgaggaca tcacggaggg cgagctcctc atcgacggga agctcgtcaa
      241 cgacgtgccc cccaaggacc gcgacatcgc gatggtcttc cagaactacg ccctctatcc
      301 ccacatgacc gtctacgaca acatggcctt cggcctcaag atccgcaagg tccccaaggc
      361 cgagatcgac acccgcgtcc gcgaggccgc ccgcatcctc gacatcgaga agctcctgga
      421 ccgccggccc aagcagctct ccggcggcca gcgccagcgc gtcgccgtcg gccgcgccat
      481 cgtccgcaag cccaaggtct tcctcttcga cgagcccctc tccaacctcg acgccaagct
      541 ccgcgtccag atgcgcgcgg agatcatcga gctccacgac cggctcgacg cgacgatgat
      601 ctacgtcacg cacgaccaga tcgaggccat gacgatgggc acgaagatcg tcgtgatgcg
      661 cgacggcaag gtccagcaga tcggctcgcc cctctacatg tacaaccacc cgatcaacaa
      721 gttcgtcgcc ggcttcatcg gctcgccgcc catgaacttc ctcaccgtga aggtcctgga
      781 ggagggcggc aagatcctcc tcgacgaggg ctccttcagg atcggcgccg cgcccgagca
      841 ccaggcggcc ctcaagccct acgtcggcaa ggaagtctcc ttcggcatca ggccggagga
      901 cctcctcctc gccgagggcg gctccgcgcc gcacggccac atcaaggcga aggtcaccgt
      961 cgtcgagccc ctcggcgcgg agatccagct ctacgcctcg accccgaccc agcccatggt
     1021 cgcgaggatc ccgccccacc acgtctacaa gtccggcgac gcgatcgagt tcgccccggt
     1081 gatggagaag gcgcggttct tcgacaggga gaccgagctt tccatcctcc ccgtcaagtt
     1141 cgacgagcag gcctgatccc ggacgccggc cttgagaggg ccgccgccgc ctccagcgag
     1201 gcgtcggcgg ccctcttcgc gcatcagggg cccgcggccg cggagtccgc gtccttgcgg
     1261 ctcccccgtc cttcgtctat agtggtactg caatgccatt caacgtagcg gagcgcgtcg
     1321 ccgtcctcat cggcgaggtc gggcgcatcg agagcgagag cgaggacatc tacctctccc
     1381 tgggccggct cttcccccgc ctggtggcgg agatgcggaa gagctccgag acggcccagg
     1441 cctcgctcga cggcttcagc tcgctcaagg agggcgcccg cggggccgcc tcccgcggcg
     1501 cgtcggccgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgcctat
     1621 ttccgcagga tccacgagcg cgactcggcc ttcctcgcgc ggatcaacga gagcatcgag
     1681 cgcctgtcct ccctcgagga gctcatcggg cgcgtccggc tcgactccga ggagatggag
     1741 atcatctccc tcaacgcgat gacggtcgcc ctcaagtcgg gcaacgcggg caaggccttc
     1801 tccgtgatca ccgatgagct caagcgcctg tccagccgca cgatctccct cacggaggag
     1861 atcaccgccc gcgggcgggc cctcctcgag tacttctccc gcctgcgcga ctccctgggc
     1921 gagctcgact ccttccaggc cgccttcttc gccgacctgg acaaggccct ctcccagggc
     1981 tacagcgagc tcgagggcga cgtgcggagc gcgctcgagg tcttcggcgg cctgctcgcc
     2041 gacgcgcagt ccgtgcgcga gcccgtgcag aacatcatgc agggcatcca gctccaggac
     2101 atcttccgcc agtccctcca gcacgtctcc atctccctgg aggaggcgcg gaagtctgcc
     2161 ggcgaggcct cggccatggg ctccggcagc gaggcgtccg ccgcgctggc ggaggagctc
     2221 gccttcgtgg ccgccgtcgc ggagctctcg ggctccctcc tcgaggagat agtcggccag
     2281 ctcgactccg ccgcgggcga gttcgcgaag gagatcgagg ccgtgtcccg cctcatcgag
     2341 gaggtcgagg gccggcgggc ggccttcatc ggcgaccccg agcgcgtcca ggaggggagc
     2401 gtcgacgcct cgcgcttctc cgcgggctcg ggccgctacc tcgccctcaa gaaggacgtc
     2461 atcgggacgg cgaggcggct ctccgagcag gtccgcgtcc tggacgagag cttcaagggc
     2521 ctcgcgggcc tcctcgcgcg cttccagaac atcgtcgtcg cctcccgcat cgaggtcgcc
     2581 aagaaccgca ccctcgcggg cgtcacgacg acggtccagg ggatgatcga gctcaccgag
     2641 cgcctgggcg cggacgtggg cggcgcgatg gcgacgacga aggacttcat caaggtggcg
     2701 agcgcggcca tcgcggagta ctcgggcggc gaggaagcgg gacgccgctc ggcccgcgag
     2761 gagtcgaagg cgggcacggc ggaaggcgac cgcctccagg ccaccctgcg gagcgtcgag
     2821 gcgggcatcg agagcctgga cggctcgcgg gtcgccgtcg cccgcgccgt cggggacttc
     2881 cgcctctaca cgcccgagtt catcgccctc atcgccgagg cccggggcgc cctggagcgc
     2941 cttcgcggcc tctccgcgga gctcaagcgg gcccaggccg acctcgccct cctcaagagc
     3001 acggtggacg aggaggcggg gccggacgcg cccaggggcg cgatccgcag cgaccgcctc
     3061 aagtcgatga tcgagcgctt cacgatcttc acgcacaagc ggacggcggg ccagatcggc
     3121 aatttcgacg tcgaggcggg cgtcgaggcc ggggacatca ccctcttcta ggggcgggcg
     3181 ccgatgttcc gccaccgcat cacccatttc gagcaggagg tcgtcaccat ccatcccggc
     3241 gagtacttcg ccacgaagga cgagacgatc atctccaccg tcctgggctc ctgcatcgcg
     3301 gtcgcgctct acgaccccgt ctcccaggcc gggggcctca accatttcat gctcccgggc
     3361 gagatcgcgc cggacgagct cgtccgcgac ccgaacgcga agtacgggat gtgggcgatg
     3421 gagctcctca tcaacgagct catgaagctc ggcgtgcggc gccgcgacct caaggccaag
     3481 gtcttcggcg gcggggccgt gctccgcttc ctggacggct cggtcagccg catcccccag
     3541 agcaacatcg atttcgcctt cgagtacctg gagaaggagg gcatccccat cctcgcctcc
     3601 gacgtgggcg ggcgcgaggc gcggaaggtc ttcctcttcc cgcggacggg caaggtcctc
     3661 atgaagcgca tcggcggcag cctggccaac ctcgtcgaga aggaggagga gcgctacctc
     3721 acgacgatcc gcacggaggc gccggagggc gagatcgtcc tcttcaagga ctagcccggc
     3781 tcggcgcggg cgcgccgccg gccatccccg tcgcaggact cgggcgacaa aaaagccggc
     3841 ctcatcggcc ggccatcgcg tcgctcatgc cgtcaatcgg ccgaggaagt cttcgaatcc
     3901 ttcttggcct tcttctcggc ccgcttctcc ttgagggtct tctggggctt cttcttctct
     3961 tcgttcttcg ccttgtcctg agatttcatg caatcctcct gtcctcgcgg actacgggct
     4021 ctcacgagcc tacgatcatt gtagcgagcc cgccggaatt ttcaaggtcg cccgcgcctc
     4081 gcggaaggac cgcttcccct tgcggagggc ggcggctttc cctaggatgg aggcgatcga
     4141 ccggcggccg cgtcagggcc gtctagccaa gggggcaagc tcatggggat cggctctttc
     4201 atggacaggt tgctcggccg cgcgcgtcct cccgcgccgg aagtggccga gcccgcgcct
     4261 ctgggccccc tctccgcgct cgaggcggcc gatccgctcg ccctggcggc ggccgactac
     4321 ctcttccccc tcgccttcca ggcggggaag acgccgatcc gcggccggcc gacctcggtc
     4381 cgctcgggcg aggcggcgct cgaggggctg cccgaggggc catggttctg gtggaaggcc
     4441 gagaccgggg caggcgcggc gggagggtac tggtgcctcc cgccttccct tccgccccgc
     4501 gacgccgagg cggccgccgc ctgggccaag actgccacgg ccctcgccgg cgccgccgcg
     4561 gcccggctca aggccctgct cgcggccgcc ccgggcctcg ctccctcctc cttcgccgcg
     4621 ggcgccttcc cgcagggcgg cgcgccgaag ctcgcgggca agagcgccct gcgcctcgat
     4681 ttccgctacg ccgcccgcgg cgccttccgt cggggcagcg ccttcacctc gccgggctac
     4741 gccgagatcc tcgccggggc tctggggccg ggccacgggc gggcgtcggc cggaggccgg
     4801 cttcaggacc ttcccgcccg ctccgaaagt cctgcgggag ccgatcccat cggagccctg
     4861 ctctccgcct ccgagtcgat cctcgcccga aacccggctt cgctcatcgc gtccaggtcc
     4921 ttctccttcg gcctccccga gggccctctc tcctacctcc cgctctacga gtgcctcaac
     4981 ctcctctcgg acgccgacct ccgcctcgtc gtgcagaacc gcctggtccc caaggcgcaa
     5041 ggcggctcct tcggcgccct cttctcctac cgggaggccg agccctccgc gcctcccgcc
     5101 ggcgcgacag ggacggcggc gcggccttcg cggcccgcgc ccggnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnggccgt cctggaggaa ggcagactcg atccggaaca
     5281 cgcggctgcc gacgcccggg acttcctcgc gcggaacgac gccgcctacg aggacctgtt
     5341 ccgcgcgacg aggagcggct ccctcgccct ctcggagcgc ggcgcggccc tggcccgcga
     5401 gctctatctc tcgatggtct acggcccgaa gaggcggggc ttcgaggctt tcctcaggga
     5461 atcgaggccg gtccaggagc tcgccgggct tcccgagcgg atagcccgcc gcgccgtcga
     5521 cctttcgggc gcgcgatccc tcgcggccgc ggtcttcggc gacccgggcg acctcgccta
     5581 cgtcgccaag tggtgctcga aggggaagcg cgcggagatc gaggacgagc agaggcgggt
     5641 ggccgcggcc ctggacgagg gcctcgccga cctcgaagca ctcaaggccg agcgcggcgc
     5701 cctcttggcc aaggcgaagg ctgtcatgga agaggagcgc cgctaggccg ggacatgcgg
     5761 cgccgggccg gctgagccgc taggccggga cctgcggcgc tgggccgcgg cctgcccgtg
     5821 cgcccaacgc agctcagccc atcgcggcgc cgatccggcg gccatgccgc ggcgcgccca
     5881 ggcgaccgga cgcgcccggc gcgcggatag gcctcggccg cgcgctccgc gcggccggcg
     5941 ccgcgccgcg agcgctcagc cgcaggacgc gggcttgcgc ggctcgacga gggcgccttc
     6001 cccctcggga aggtagacct tggagtcggg cggcaccgag tgcgtgagcc agacgttgcc
     6061 gccgatgacg gagcgggcgc cgatctccgt gcagccgccc aggatcgtcg cccccgagta
     6121 gatcgtcacg ccgtccccga tcgtcggatg gcgcttggtc tccccgtcct ccttcttgac
     6181 cgagagggcg ccgagggtga ctccctggta gagcttgacg tcgtcgccga tgacggtggt
     6241 ctcgccgacg acgacgcccg tcccgtggtc gatgaagaac cgctttccga tcgtcgcgcc
     6301 cggatggatg tctatgcccg tcttcccgtg caccgactcg ctcatcatgc gcgggatgag
     6361 ggggaggccg gccttccaga aatggtgggc gatgcggtgg acgacgaggg cctcgaggcc
     6421 gggataggcg aggatcacct cggcgacgct cttggccgcc ggatccccct tgaaggccgc
     6481 ccccacgtcc tccatgagga tagcgcgcag ggcgggcagg gcctcgaaga agcccaggac
     6541 caggtcgcgg gcctccaggt ggcaggcggc cgggcagccg ggcttcccct ccaggcggta
     6601 gcggtactcg aggctccgct ccgcctcgcg cgaaagcacc ttcgcggcgc ggtggacgag
     6661 ctccccggcc acgaggccga ggttggagtc gtctatcccg tcctcctggc ggaagccggg
     6721 gaagaccaga gtctccaggt cctcgagggc gaggacgacc gcggcccggg agggcaggtt
     6781 gggcccgccc gagtggttca cgccgccgtc gcgctcgtag gaggagagga gtccctccac
     6841 cgccgcctcg agggcctcca cgctaggctc ccgccgccgc cggcttctcg ccgggctcgc
     6901 ccttcgtcac cttgcccatg aggacgaagg cggcgaactc gcagaaggcc gcgaacacga
     6961 agatgccggg atagcccacg aagtcgatga tggcgccggt gaccggcggc gaggagatgg
     7021 ccgcggtctg gctgaaggtg tagtagaggc ccgtgtagag gccgatcgag ccgaaggtcg
     7081 ccatctgcca gagcatcggg aaggagttgg tgatgatggt gatccagaag acgccgtaga
     7141 cgaacatcat cgcgaggagg aggttgaagc gcagggcggc gcccagcggg gcggcgaggg
     7201 ggctcgcgac gaaggccgtg aggcccagga tcgcgacgag gacgaggagt gagcccctga
     7261 tcgccttccg cctgccgatg cggtgggcga catagcccga ggggatggcg aagagggtct
     7321 gggcgatggc caccgcggag gcggggaggg ccgcctttcc gttggaggcg atctcgaaga
     7381 cgtccttgca gagcttgccg atgaagggga ggatgccctg gtagccgagg aaccagaaga
     7441 atagtgcgag gaggatccag agggccgact tgtccttctc gccggcgatc cccttgaggg
     7501 ccttcgcgag gggcaccttc tcctcttttt cctcctcctc gcggctccgc tcgcggacga
     7561 aggagaagac gaggacggtg gccaggacga cgaggacgcc ggcgatgggg aagggcaggg
     7621 cgtccttggt ccttccgagg agcggcagga cgatgtccag gtccatgagc cgcgcgaggc
     7681 ccaccgtgga gatgatcgcg cccagggcgc ccatggtgtt gatgatgccg ttggcctccg
     7741 agcggtagtc gcccgggatg gtgtccggca tcagggcgac gacggggccg cggacggact
     7801 gcttgaagat gttgaggccg aagatcacga ggaggagggc tccgagcgag cggaaggccg
     7861 cgtaggggat gagggagaag cagagggccg agagggggag gaggacgacg atgaagggca
     7921 tccgcctgcc gatcctcgtc ctcgtgttgt cggaccagag cgagacgaag gggatgagga
     7981 agaccgcgag gatgttgtcg agggtcatga gcccgccgac gatccagttc ttcgcgatgt
     8041 acttcccgag gaagatcggc acgtaggtgt cgtagaggat gtccatgagg cccatcgtga
     8101 agaagccgaa gccgatgagg aaggtcaggc cgaggtactt gtcgaagcct tttttcgagc
     8161 cgcccatgcg atctcctttc tggtatccgc cgtatgggac gcgcctgccc ccgcgggggc
     8221 cccgctctcc cttgtagcct gtccgctccg ggcgcgccgc cctaggcgag gcgcagcgtc
     8281 ccgaggggcc tcgccccgat cgggtggacc gagcccgggc cgaagtagct ctccatgagc
     8341 gccttccagc cctcgaagcg ggccaggggc acgtaggcct ggatggttcc cgcgaagcct
     8401 ccgccgtgga cccggaccgc cccctctccc gcgaggaagc gctcggagag ggccagggcg
     8461 agggggaggc cctgttcgtg aggggcctcg ggcgggtaga ggttctggag gaggcgccag
     8521 gacgagtcgc ccgaggcgcg gacgaggccg aggtaggcgg cgacgtcgtt ccgctccagg
     8581 gcggcgacca tcgcggggac ccgcgcgtcc tccgcggcga agtggagggc gcggaggagg
     8641 gcgcggtcgc ccagggccgc cctgatcgcc ggccccctct ccacgaggag ggcgggatcg
     8701 acctctcgga ggacggcttt cccgaggaag gaggcgactg ccttcatctc cgccgggacg
     8761 gcggcgtagt ccgccgtgag gtcggcgtgg ctgccgcccg tgttgaccac gcagaggacg
     8821 aggccggcct tggcgaaggc gaaggggatg ctccgcacgg cgggggccga gggaagcgcg
     8881 aagtctatcg cgacgacgcc tccgactgcg gaggcggtct ggtccatgag gccgcagggc
     8941 ttgccgaaga agcggttctc cgcgtactgg ccgatcttcg cgagctccac ggcgtcgatg
     9001 cccttctccc gggcggcggc cgagcccagg cccgcgcccg ccaggtcggc caggatggtc
     9061 ccgaagagga cctccacggc ggccgagctc gagaggcccg agccggggag gacggtcgag
     9121 tccatggccg cgtcgaagcc gcctatcgcg acgccgcggt cggcgaggcc gcgggccacg
     9181 ccgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcctc gtcctcgatc
     9301 atcttcctgg ctgcgccgac ggtcggcatg cgtgactcct gcccgcgagg cttcgcttga
     9361 ccgcgccgcc gccccggttc cggggccggc ggcggcgtct ccccgcggcg gggccattgt
     9421 agcagaaagg gcgcccgcgg gaaagaagcg ggggcggaaa gcgggagaaa gggatggcgg
     9481 cgcgagcatt gatttgacct tgggctcccg ttgctgtaag ctcgaaccgt gaagcagagc
     9541 gagaagccgt cgcgcgcgac ccctctcctg gccgccctcc tcctgggcgg cctcgccgtc
     9601 gcctccgcct tctacttccg ctcctacgag gcccagaccg cgccgtccct cctcgcctcc
     9661 gacgccctcg tccgcgtgga aaagggcgcc tacggcctcg ccttcctccc ggcggcggga
     9721 gccgggagcc tgggcctcgc cttctatccc ggcgcccgcg tcccccccga ggcctacgcc
     9781 tacctggcgc gcgccctcgc cgcgcagggc tacccgaccg tcctcctttc gacccccctc
     9841 aacttcgccg tcttcgcgcc ggggaaggcc ggcgcggccg cccgggcatt ccccgaggtg
     9901 ggcgcctggg tcctgggcgg gcattccctg ggcggcgcga tggccgcggc cagggcggcc
     9961 aaggctccgg gctcggcggc cggcctcctc ctcctcgccg cctatcccgg ggggggcgcc
    10021 gacctctcgg gctcgggcct gcccgtcctg agcgtctccg ccacgaacga cggactcgcg
    10081 acgccggcga aggtgcaggc ggccaggagc ctgcttccgc cgggcgcgcg ccatgtcgtc
    10141 ctcgcgggag ggaaccacgc gcaattcggg gagtacgggt cccaggccgg ggacgggacg
    10201 gccgagcttc cggcggcggc ccagcggcgc gcggtcgtcc aagaggcggc cgggttcctc
    10261 ggcgccgtcc tggccgcggt gcggccctag gagggcggga tgaacagcgt ggacgcgatc
    10321 atcgtcgggg cggagtcggg catcggcagg cgctggctcg cgaagctcag ggcggcaacc
    10381 aacctgaagg ccgtcaatgc cctgggcgac gaggagctcc tctccgtcca cgcggagatc
    10441 gtggccaagc tgg
//
LOCUS       DAOOSJ010000068        10384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4177_length_10384_cov_5.631717, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000068 DAOOSJ010000000
VERSION     DAOOSJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_10384_cov_5.631717"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLG14_04910"
     CDS             <1..290
                     /locus_tag="PLG14_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48507.1"
                     /translation="FFQFYEGKLWVMILVMDQRKMDHYSVYSSLRAKYGEPTLLDPRE
                     VRWEDEATRVALERPLTLRYLDLATYRSLREGSEAREAVEELERREFLGDL"
     gene            401..1345
                     /locus_tag="PLG14_04915"
     CDS             401..1345
                     /locus_tag="PLG14_04915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOX48508.1"
                     /translation="MVNESAALRGRILARLRASPEPVSGNELSGELGVSRVAVWKHLE
                     ALREAGYGLEAGRSGYRLAADGDFLHPWEFPGREARVVRYERTDSTMDRALELALRSP
                     SCRAIVVAEAQSAGRGRRGRRWASPRGGLFATLVAEPATAPWRAERAAMAGGVALCLA
                     LRELTGEPFSLEWPNDVYLAGRKAGGLLVEYLAEGESLRLLDLGLGVNVANRAPGGNA
                     FSLSELPSPAPTRRALLSAFLDRYETLDLEWPGLAEAWTGLSSSPGRIAVSRDEDRRL
                     GRVLRVDDEGRLVVELTGGGTKSFRPSEARIESKEQAS"
     gene            1342..2013
                     /locus_tag="PLG14_04920"
     CDS             1342..2013
                     /locus_tag="PLG14_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOX48509.1"
                     /translation="MSKNRGLVPPVLSCLFAALISVGAYIAVPLPGTPVPVVLQNLFI
                     MLAALLLGPAWGLASVLVYLSLGALGLPVFAGGAGGFARFLGPTGGYLVGYVPAALAI
                     GLVSRLGARRRWWRDALALLAGELLVYGIGLPWLKAAIHADWAKALSGGLLPFLPGDA
                     VKAALAVVLAGRLSPLLERALPRRGGHAAEAGVADDGAADGGTADGDAADDGAAGRGA
                     PGYRG"
     gene            2006..2761
                     /locus_tag="PLG14_04925"
     CDS             2006..2761
                     /locus_tag="PLG14_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48510.1"
                     /translation="MAELARVEALVHRFPDGGLGLDGVDLRLEEGDFLVLAGRNGSGK
                     SLLARHLIGLARPSSGRVLCRGRHVAADPLEARRTIGYVFQDADAQIVGQTLLEDAAF
                     GPSNLGLPRAEIAARAREALAQVGLSALENRRPDSLSGGERRRLAIAGVLALRPACVL
                     LDEPFANLDLPAIRSVLGVIADLHSGGRTVVVVTHELEKVLAHATRLAVMDQGRVVFD
                     GAPDGLARARYEELGLMDPFRSGAALKELSWLR"
     gene            2749..3513
                     /locus_tag="PLG14_04930"
     CDS             2749..3513
                     /locus_tag="PLG14_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOX48511.1"
                     /translation="MASVGPFRFEWGDYPARRLHPLSKLAALVALSVSSTASRGAWLP
                     FLALAALGALASAGAFRPGLLKGLSALARDARFLLALSLFVAAFRVVDPWGPSLLRLE
                     ELAPALLYVARLALVFAFAEAFFRSTSAEELAAAATLLARRLFRRVDLDPGLYLSLAV
                     NFIPRCFEAYGRAREAALARGYGGRRGRRPRFASSLAVLESFIAGSIRAALSAAEALE
                     SRGYSPARVLGGKPFRPADAALAAAASGLAVLALAL"
     gene            complement(3762..5870)
                     /locus_tag="PLG14_04935"
     CDS             complement(3762..5870)
                     /locus_tag="PLG14_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48512.1"
                     /translation="MADFFETDEVTKGYDSRIAGRILRYLRPYRLLAAAALLALAAGT
                     AGELYLPVLVRRTVDDALMVSWFAVDARAASLPEGRALAIGPGDPVVAGRAYLKASRL
                     SAVSGEDRRSLEASGLLDPEPRYLVQIRPGDAAQAGALAAAGASPLPGEAYAAMPVAE
                     LRRLAPAEAAAVRRGDSRTVAANVAILFFTLAVVLLATFLQVYLASLIGQRIMKDLRM
                     ELFRHASTRSLAFLSRQPVGRLVTRMTSDVETINQFFTDVVAAFLKDAALMAGVLVLL
                     FVLDRRLALATLASLPPVAVATAISRRKARDAFRRQRTWLSKVNSYLAERLSGIAVVQ
                     LFAREAATRAEFEDRDRELMKANLGEMYVFATFRPLVDFFASLSTAVIIYVGASMFKA
                     HVLSLGTLIAFINLIRMFYSPVQDISEKYTLLQSAMAGGERVFKLLDSDERIPDEPRA
                     AMPSPLRGSIEIDSVRFAYKPGEWILKDLSLSVRPGEMVAIVGATGAGKTTVANLITR
                     LWDVQEGEIRLDGVPVRDLPLADLRRAVQPVLQDVFLFSGSIEDNLRLGTEVPRERML
                     EAARAVHADEFIESLPEGYATKLSEGATNISSGQRQLLSFARVLAHDPAVVILDEATS
                     SVDTETERLIQRGLEGLLAGRTSLVIAHRLSTIRHADRIVVLAGGRVAEEGRHEELLE
                     KRGLYWNLYRLQYGGEVERA"
     gene            complement(5875..7626)
                     /locus_tag="PLG14_04940"
     CDS             complement(5875..7626)
                     /locus_tag="PLG14_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48513.1"
                     /translation="MLKEFRTLLPYLRTYRRKYAAGLFCLVAVDAAQLLLPQIVKRAV
                     DAVSSGGFELSFIARLAALMVGIAAFVSAGRFLWRFFIHGASRRIETEMRDRLFGHLL
                     SLSGGFYQENKTGDLMARATNDMSAIRQATGMGFVTFVDGAFMSLAILAIMIAQNPRT
                     ALLTIIPLPLITLLIVFFGGMVGKRFKRVQEIFSRLSEIAQETMAGIRVVKAFVKEER
                     FASDFSKANDEYRVASMGLAKIFGFFFPLIGFLSGLTTLILLGVGGAAVIENRMSAGD
                     IVAMLAYLEMLVWPMIGAGFTVNTLQRGAASLKRVNEVLEAKPAIASLPGSSSAAPRG
                     GLEFRSLSFSYPGASSPALLEVSAALPAGSTLGILGRVGSGKSTLLRLLPRLLDPPQG
                     TVLVGGRDIRDYELSALRSAFGFVPQDSFLFSDTIAANVRYGAPALPEERFRRIVEIS
                     AIARDVADFPQGWDTLVGEKGLTLSGGQKQRVAIARALAADPEILVFDDALSAVDAET
                     EDRILGGLIEERRGRTNVIVSHRVSTLRHADLIIVLEGGRISQRGSHAELLADESGFY
                     AEIARLQELERVAEKGE"
     gene            7739..8677
                     /locus_tag="PLG14_04945"
     CDS             7739..8677
                     /locus_tag="PLG14_04945"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOX48514.1"
                     /translation="MKEKLRRPSRRAARAAVPALAALLSLSSCAVLRAGGSVEKVFLA
                     EEDLVIAEATLPVLIKASEALLAGDPKDGGKAVTTASLYVMYASAFVSGPADLLPDEE
                     YEAKGRAARRAQALYRRASRLLLPLLEDRAPGFLAADSDESAASKLSRFRKDDLPLLY
                     WTAASLLGAFSIDPLSFEGAKGLLPARRLLARASALDPAWNSGALEELNLSLYASLPD
                     YLGGSLEEAEKAYLRALEYSGGKSASVYVTYATSIAVVHDDYAAFKDALRKALAVDDE
                     ALPGSRLPNALAKDRAERLLAQPERYFLTTEGDPAP"
     gene            8674..9684
                     /gene="dctP"
                     /locus_tag="PLG14_04950"
     CDS             8674..9684
                     /gene="dctP"
                     /locus_tag="PLG14_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOX48515.1"
                     /translation="MKRLSLPASLIAAAYLACAAAGAGAQALTLRVASYVPPNSTWDI
                     GLKKLAADFDRISGGRVKLVFPQSLRASSDSDIIQKMKFGVDGALITTMGVAELYPDS
                     LALAIPSIIRSDEEYDAVLAVVEPLIRKKLADRYVVLALAKGGWIRYFSKAPIVYPED
                     LVKARMAVSADDDKIQRLLQSVGTRTVKADMAALILQLSSNTVDAFYLSPVQLASLWT
                     QYKSQVSYMSPFKVSPFLGAVIFTKQSWDKVPAEFRPRLEEATREAARVMARESAKLE
                     EDAIAAMVREGLKVPAYPADAEPKWNALYAEKRDSLVATMFSADFLEAMRKALASARS
                     KR"
     gene            9693..>10384
                     /locus_tag="PLG14_04955"
     CDS             9693..>10384
                     /locus_tag="PLG14_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48516.1"
                     /translation="MSGGSGSRAGGASARALSAVKAAARWILDGSALVALATLSVLPV
                     LDLVLARLFWRGIPSSGTVLQYSILALAFLSAAIATREGRQLSLGSGAKDGGRRSPGK
                     GLPAWRRLLARAVALGPDFARAVEAGAVAAFAWTSLSFVFAAFDKSSRVLFLPERWFV
                     AAMPFGFFLMLAFVLARKGRGGRLAALAGALAGTFLAASAIANLASVLEAESAVPGGL
                     ASALGPVSAAAGA"
BASE COUNT         1415 a         3826 c         3620 g         1523 t
ORIGIN      
        1 ccttcttcca gttctacgag ggcaagctct gggtcatgat cctcgtcatg gaccagcgga
       61 agatggacca ttactccgtc tacagctccc ttcgcgccaa gtacggcgag ccgaccctcc
      121 tcgatccccg ggaagtccgc tgggaggacg aggccaccag ggtcgccctg gagcgcccgc
      181 tcaccctgcg ctacctggac ctggccacct accggagcct cagggagggc agcgaggcca
      241 gggaggccgt cgaggagctc gagcgcaggg aattcctcgg cgatctctag gaccttcccg
      301 tcgccgcgcc gcgggagcgg caggccgcct cggggctagg cccgcgcctc tcccttcgcc
      361 cttgacgcga cgccgtccat tcgttaacct cgcttcgatc atggttaacg aaagcgcggc
      421 gctccgcggc cgcatcctcg ctcgcctgcg cgcctcgccc gaacccgtct cgggcaacga
      481 gctctccggc gagctcggcg tgagccgcgt cgccgtctgg aagcacctgg aggcccttcg
      541 cgaggcgggc tacggcctcg aggccggccg ctcgggctac cgcctggccg cggacggcga
      601 cttcctccac ccctgggagt tccccggccg cgaggcgcgc gtcgtccgct acgagcggac
      661 cgactcgacc atggaccgcg ccctcgagct cgccctgcgc tctccctcct gccgcgcgat
      721 cgtcgtcgcc gaggcgcaga gcgccggccg cggcaggcgg ggccgtcgct gggcctcccc
      781 gcggggcggc ctcttcgcga ccctggtggc cgagcccgcg accgcgccct ggcgggccga
      841 gcgggcggcg atggccggcg gggtggccct ctgcctggcc ctccgcgagc tcacgggcga
      901 gcccttctcc ctcgagtggc ccaacgacgt ctacctggcc gggcgcaaag ccggcggact
      961 cctcgtcgag tacctcgcgg agggcgagtc cctgcgcctc ctcgacctgg gcctcggggt
     1021 caacgtggcc aaccgggcgc ccggcgggaa cgcgttctcc ctctccgaac tcccctcccc
     1081 cgcgccgacg cggcgcgccc tcctctcggc cttcctcgac cgctacgaga cgctcgacct
     1141 ggaatggccc ggcctcgccg aggcctggac cggcctttcc tcctcgccgg gaaggatagc
     1201 cgtctcccgc gacgaggacc ggcgcctggg cagggtcctc cgggtggacg acgagggccg
     1261 cctcgtcgtg gaactcactg gcggagggac gaagtccttc aggccttccg aagcgcgaat
     1321 cgaatccaag gagcaagcat cgtgagcaag aaccgcggcc tcgtcccccc cgtcctctcc
     1381 tgcctcttcg ccgccctcat ctccgtcggc gcctacatcg ccgtgcccct accgggcacg
     1441 cccgtgccgg tcgtcctgca gaacctcttc atcatgctcg ccgccctcct cctgggaccg
     1501 gcctgggggc tcgcctccgt cctcgtatac ctctcgctgg gcgccctggg cctcccggtc
     1561 ttcgccggcg gggcgggcgg cttcgcgcgc ttcctcgggc ccacgggcgg ctatctcgtc
     1621 ggctacgtcc ctgcggccct cgccatcggc ctcgtctcgc gcctgggcgc gaggcggcgc
     1681 tggtggaggg acgcgctggc cctcctcgcg ggggaactcc tcgtctacgg catcggcctt
     1741 ccctggctca aggccgcgat ccacgcggac tgggccaagg ccctctcagg cggcctcctg
     1801 cccttcctcc ccggcgacgc ggtcaaggcg gccctcgccg tcgtcctcgc ggggaggctc
     1861 tcccccctcc tcgagcgcgc cctgccccga cgaggcggcc atgcggccga agcaggcgtg
     1921 gcggacgacg gcgcggccga tggcggcacc gcggatggcg acgccgcgga cgatggcgcg
     1981 gcgggtcgcg gcgccccggg ctaccgtggc tgagctcgcc cgcgtcgagg ccctggtcca
     2041 ccgcttcccg gacggagggc tcggcctgga cggcgtcgac ttgcgcctgg aggagggcga
     2101 tttcctcgtg ctggccggcc gcaacggctc ggggaagagc ctcctcgcgc gccacctcat
     2161 cggcctggcg cggccgagct cgggaagggt cctgtgccgg ggccgccacg tcgcggcaga
     2221 tcccctcgag gcccggagga cgataggcta cgtcttccag gacgccgacg cccagatcgt
     2281 gggccagacc ctcctcgaag acgccgcctt cggcccctcc aacctgggcc tgccgcgcgc
     2341 cgagatcgcg gcgcgggccc gggaggccct ggcccaggtc ggcctttccg ccctggaaaa
     2401 ccgccggccg gacagcctct ccggcgggga aagacggagg ctcgcgatag ccggcgtcct
     2461 cgccctccgc cccgcctgcg tccttctcga cgagcccttc gccaacctgg acctgcccgc
     2521 catccggagc gtgctcggcg tgatcgccga cctgcattcg ggcggccgga cggtcgtcgt
     2581 cgtgacccac gagctggaga aggtgctggc ccacgcgaca aggctcgcgg tgatggacca
     2641 gggccgcgtc gtcttcgacg gggcgcccga cggcctcgcg cgcgcccgct acgaggagct
     2701 cgggctcatg gatcccttcc gctcgggcgc ggcgctcaag gagctttctt ggcttcggta
     2761 ggccccttcc gcttcgagtg gggcgactac cccgccaggc gcctccaccc gctctccaag
     2821 ctcgcggccc tcgtcgcgct ctccgtctcg tccaccgcgt ctaggggcgc ctggcttccc
     2881 ttcctcgccc tcgccgccct gggcgccctg gcctcggccg gcgccttccg ccctggcctc
     2941 ctcaagggac tgtcggccct ggcccgcgac gcgcgcttcc tcctggcctt aagcctcttc
     3001 gtcgcggcct tccgcgtcgt cgatccttgg ggcccctccc tcctgcggct cgaggagctg
     3061 gcgcctgccc tcctctacgt cgcgaggctc gcgctggtct tcgccttcgc ggaggccttc
     3121 ttccgctcga cctcggcgga agagctggcg gccgcggcga ccttgctcgc gcgccgcctc
     3181 tttcgccggg tggacctgga tccggggctc tacctgagcc tagccgtcaa cttcatcccc
     3241 cgctgcttcg aggcctacgg acgggccagg gaggccgccc tcgcccgcgg ttacggcggc
     3301 aggcggggca ggcgcccgcg cttcgcctcc tccctcgccg tcctcgagtc cttcatcgcc
     3361 ggcagcatcc gcgcggcgct ctccgccgcg gaggccctgg agtcgcgggg ctacagtccg
     3421 gcacgggtcc tcggaggcaa gcccttccgc cccgcggacg ccgccctcgc ggcggcggcc
     3481 tcgggcctcg ccgtcctggc cctggcgctc taggcgcggc ggccgtcgcc gcagcgggtt
     3541 gcgcggctga tcgctcgcgg cgctcggccc tgcgcatcgc gcgcggcgca tggagcgggc
     3601 cccgcggcgc cgccgccgcg cgcggcgcgt tccgcggcga gcggcgcttg gcgcggctcg
     3661 gtgcgcggcg ggcgcgcgcg gcccagccca tcgcccgcgg cgcctgttcc ggcccgccgc
     3721 gctcggccca aggcgcggga cgctaggcgc cgtcgccgcg cctatgcgcg ctcgacctcg
     3781 cctccgtact ggagccggta taggttccag tagaggcccc tcttctccag gagctcctcg
     3841 tgccgcccct cctccgcgac gcgcccgccc gcgaggacga cgatcctgtc ggcgtgcctg
     3901 atcgtcgaga ggcggtgggc gatgacgagg ctcgtcctcc ccgcgagcag cccctcgaga
     3961 ccgcgctgga tgaggcgctc cgtctccgtg tccaccgagc tcgtcgcctc gtccaggatg
     4021 accaccgccg gatcgtgggc gaggacgcgg gcgaaggaga ggagctggcg ctggcccgag
     4081 gagatgttcg tcgcgccctc ggagagcttc gtggcgtagc cctcgggcag ggactcgatg
     4141 aactcgtcgg catggacggc tctggccgcc tcgagcatgc gctccctcgg cacctcggtc
     4201 ccgagcctga ggttgtcttc tatgcttcca gagaagagga agacgtcctg gaggacgggc
     4261 tggaccgccc gccgcaagtc cgcgagggga aggtcgcgga ccggcacgcc gtccaggcgg
     4321 atctcgccct cctgcacgtc ccagagccgc gttatgagat tcgccaccgt ggtcttgccc
     4381 gcgcccgtcg cgccgacgat ggccaccatc tcgccgggcc tgacggagag ggagaggtcc
     4441 ttgaggatcc actcgcccgg cttgtaggcg aagcgcacgc tgtcgatctc gatgctcccc
     4501 cgcagggggg acggcatcgc ggccctgggc tcgtcgggga tccgctcatc cgagtccagg
     4561 agcttgaaga cccgctcgcc cccggccatc gcgctctgga ggagggtgta cttctccgag
     4621 atgtcctgga ccggggagta gaacatcctg atgaggttga tgaaggcgat gagggtgccc
     4681 agggagagga cgtgcgcctt gaacatcgag gcgcccacgt agatgatcac cgcggtggag
     4741 aggctagcga agaagtcgac gagggggcgg aaggtcgcga agacgtacat ctcgcccagg
     4801 ttcgccttca tgagctcgcg gtcccgatcc tcgaactccg cccgggtcgc cgcctccctc
     4861 gcgaagagct ggacgacggc gatgcccgag aggcgctcgg cgaggtagga attcaccttg
     4921 gagagccagg tcctctgcct gcggaaggcg tcgcgggcct tccgccgcga aatggccgtc
     4981 gcgacggcga cggggggcag ggaggcgagg gtcgcgaggg cgaggcgccg gtccaggacg
     5041 aagaggagga cgaggacgcc cgccatcagg gccgcgtcct tgaggaaggc ggccacgacg
     5101 tcggtgaaga actggttgat cgtctccacg tcgctcgtca tcctcgtgac gaggcggccc
     5161 acgggctggc gggagaggaa ggcgagcgac cgcgtcgagg cgtgcctgaa gagctccatc
     5221 ctgaggtcct tcatgatccg ctggccgatg aggctcgcga ggtagacctg gaggaaggtg
     5281 gcgaggagga ccacggccag ggtgaaaaag aggatcgcca cgttggccgc caccgtccgg
     5341 gaatcccccc ggcggaccgc cgccgcctcc gcgggggcga gcctccggag ctccgcgacg
     5401 ggcatggccg cgtaggcctc gcctgggagg ggcgaggcgc cggccgcggc gagggccccg
     5461 gcctgtgccg cgtcgcccgg ccggatctgc acgaggtagc gcggctcggg gtcgaggagg
     5521 cccgaagcct cgagggagcg gcggtcctcg ccggagacgg cgctcagccg cgaggccttg
     5581 aggtaggccc ggccggccac gacggggtct cccggcccga tggcgagggc ccggccctcg
     5641 ggcagggacg cggcgcgggc gtcgacggcg aaccaggaca ccatgagggc gtcgtccacg
     5701 gtccggcgga cgaggacggg gaggtagagc tctcccgccg tgcccgccgc cagggccagc
     5761 aaggccgcgg cggcgagtag gcgatagggc ctcaggtagc ggaggatccg ccccgcgatg
     5821 cgggaatcgt agcccttggt gacctcgtcg gtctcgaaaa aatcggccat ggccctattc
     5881 ccccttctcc gcgacgcgct cgagctcctg gagcctggct atctccgcgt agaagcccga
     5941 ctcgtccgcg aggagctcgg cgtggcttcc ccgctgggag atccggcccc cctcgaggac
     6001 gatgatgagg tcggcgtgcc gcagggtgga cacgcggtgg gagacgatga cgttcgtcct
     6061 gccgcggcgc tcctcgatga ggcccccgag gatccggtcc tccgtctccg cgtccacggc
     6121 ggagagggcg tcgtcgaaga cgaggatctc cgggtcggcg gccagggccc tcgcgatcgc
     6181 gacccgctgc ttctggccgc ccgagagggt gagccccttc tcgcccacca gcgtgtccca
     6241 gccctgcggg aagtccgcga cgtcgcgggc gatcgcggag atctccacga tccggcggaa
     6301 gcgctcctcc gggagggccg gcgccccgta gcggacgttg gccgctatcg tatcggagaa
     6361 gaggaaggag tcctggggca cgaagccgaa tgcgctccgg agggcggaaa gctcgtagtc
     6421 gcggatgtcc ctgcctccca cgaggacggt cccctgcggc gggtcgagca gtcgggggag
     6481 gagcctgagg agcgtggact tccccgagcc gacgcggccc aggatgccga gggtcgaccc
     6541 ggcggggagc gccgccgaga cctcgaggag agccggcgag gaggcgccgg gataggagaa
     6601 ggagagggat cggaactcga gcccgccccg gggcgcggcg gaggaggagc ccggcaggct
     6661 cgcgatggcc ggcttcgctt ccagcacctc gttgacgcgc ttgaggctcg ccgctccgcg
     6721 ctgcagggtg ttcaccgtga agcccgcgcc gatcatcggc cagacgagca tctccagata
     6781 ggcgagcatg gcgacgatgt cgccggcgct catcctgttc tctatgacgg cggcgccgcc
     6841 aacgcccagc aggatgaggg tggtgaggcc cgagaggaag cctatgaggg gaaagaagaa
     6901 gccgaagatc ttcgcgagcc ccatggaggc cacgcggtac tcgtcgttgg ccttggagaa
     6961 gtccgaggcg aagcgctcct ccttcacgaa ggccttgacc acgcggatgc ctgccatggt
     7021 ctcctgggcg atctcggaga ggcgcgagaa gatctcctgg acccgcttga atcgctttcc
     7081 caccattccg ccgaagaaga cgatgaggag ggtgatgagg gggaggggga tgatggtgag
     7141 gagggccgtc cgcgggttct gggcgatcat gatcgcgagg atggccaggg acatgaaggc
     7201 gccgtccacg aaggtgacga agcccatccc ggtggcctgg cggatcgcgg acatgtcgtt
     7261 cgtggccctg gccatgaggt cgccggtctt gttctcctgg tagaagcccc ccgagaggga
     7321 gaggaggtgg ccgaagagcc tgtcgcgcat ctccgtctcg atgcgccgcg aggctccgtg
     7381 gatgaagaag cgccaaagga agcgccccgc cgagacgaag gccgcgatgc cgaccatcag
     7441 ggccgcgagc cgggcgatga aggacagctc gaagccgccg gaggagacgg cgtcgacggc
     7501 gcgcttcacg atctggggga ggaggagctg ggcggcgtcg accgcgacga ggcagaaaag
     7561 cccggcggcg tacttccggc ggtatgtacg caagtagggc aggagcgtcc tgaattcctt
     7621 gagcatcgct agaatgtacc gcggccgggc tcggggggac aagctcctcc gcccgggccg
     7681 ggcgggcgcg gcgacaattc cgattgacag gccggtcggc cgaggcgata gttgaggggt
     7741 gaaagagaag cttcgccgcc catcgcggcg cgccgcccgg gccgcggtgc cggccctcgc
     7801 cgccctgctt tccctctcct cctgcgccgt cctcagggcg ggcggcagcg tggagaaggt
     7861 cttcctcgcg gaagaggacc tcgtcatcgc ggaggcaacg ctgcccgtcc tcatcaaggc
     7921 gagcgaggcc ctcctcgcgg gagacccgaa ggacggcggg aaggccgtga cgacggcctc
     7981 gctctacgtg atgtacgcga gcgccttcgt ctcgggaccg gcggacctcc ttcccgacga
     8041 ggagtacgag gccaagggcc gcgcggcgcg gcgggcccag gcgctctaca ggcgcgcgag
     8101 ccgcctcctc ctccccctcc tcgaggacag ggcgccgggc ttcctggccg ccgactccga
     8161 cgagtccgcc gcgtccaagc tctcgcgctt ccggaaggac gacctccccc tcctctactg
     8221 gaccgccgcc tccctcctcg gcgccttctc catcgacccg ctgtccttcg aaggcgcgaa
     8281 gggactcctg ccggccagga gactcctcgc gcgcgcctcg gccctcgatc ccgcctggaa
     8341 ctccggggcc ctcgaggagc tcaacctgag cctctacgcg tccctccccg actacctggg
     8401 cgggagcctc gaggaggccg agaaggccta tctccgcgct ctcgagtact ccggcggcaa
     8461 gtccgcctcg gtctacgtga cctacgccac ctcgatcgcc gtcgtccacg acgactacgc
     8521 ggccttcaag gacgccctcc gcaaggccct ggccgtggac gacgaggccc tgcccggctc
     8581 ccgcctgccg aacgcgctcg cgaaggacag ggccgagcgc ctcctcgccc agccggagcg
     8641 ctacttcctg accaccgaag gagaccccgc accatgaaac gccttagcct gcccgcctcc
     8701 ctcatcgcgg ccgcctatct cgcctgcgcg gcggcgggcg cgggcgccca ggccctcacc
     8761 ctgagggtgg cgtcctacgt cccccccaac agcacctggg acatcggcct caagaagctg
     8821 gccgccgact tcgacaggat ctcgggcggg agggtgaagc tcgtcttccc gcagagcctc
     8881 agggcctcga gcgactcgga catcatccag aagatgaagt tcggcgtgga cggagccctc
     8941 atcacgacca tgggcgtcgc ggagctctac ccagactccc tcgccctggc catcccctcg
     9001 atcatccgga gcgacgagga atacgacgcc gtcctcgccg tcgtggagcc cctcatccgg
     9061 aagaagctcg ccgaccgcta cgtggtcctg gccctcgcca agggcggctg gatccgctac
     9121 ttctccaagg cgcccatcgt ctaccccgag gacctcgtga aggcgcggat ggcggtgagc
     9181 gcggacgacg acaagatcca gagactcctg cagagcgtcg gcacgaggac ggtgaaggcg
     9241 gacatggccg ccctcatcct ccagctctcc tccaatacgg tcgacgcctt ctacctgagc
     9301 cccgtgcagc tcgcgagcct ctggacgcaa tacaagagcc aggtctccta catgagcccc
     9361 ttcaaggtct cccccttcct gggcgccgtc atcttcacca agcagagctg ggacaaggtt
     9421 cccgccgagt tccggccccg cctggaggag gcgacccgcg aggccgcccg cgtcatggcg
     9481 agggaaagcg ccaagctcga agaagacgcg atagccgcca tggtccggga gggcctcaag
     9541 gtgccggcct accccgccga cgccgagccc aaatggaacg cgttgtacgc ggagaagcgc
     9601 gacagcctgg tcgcgaccat gttctccgcg gatttcctcg aggccatgcg caaggccctc
     9661 gcctcggcga gaagcaagcg ttaggcggcg gcatgagcgg cggctctgga tcccgggcgg
     9721 gcggcgcctc cgcccgcgcc ttgtcggccg tcaaggccgc cgcgcgctgg atcctggacg
     9781 gatcggccct cgtagccttg gcgacgctgt ccgtcctgcc cgtcctggac ctcgtcctgg
     9841 cccggctctt ctggcggggc atcccctcgt ccggaacggt gctgcagtac tccatactcg
     9901 ccctcgcctt cctctccgcc gccatcgcga ccagggaggg caggcagctc tccctcggct
     9961 cgggcgccaa ggacggcggc cggcgctccc cgggaaaagg gcttccggcc tggaggcggc
    10021 tcctcgcccg cgccgtcgcc ctggggcccg acttcgcccg cgcggtcgag gccggggcgg
    10081 tcgccgcatt cgcctggact tcgctctcct tcgtcttcgc ggccttcgac aagagctctc
    10141 gcgtcctctt ccttccggag cgctggttcg tcgcggcgat gcccttcggc ttcttcctga
    10201 tgctcgcctt cgtcctcgcg cggaagggcc gggggggaag gctggccgca ttggccggcg
    10261 ccctggcggg aaccttcctc gcggcctcgg cgatcgccaa cctcgcctcc gtcctcgaag
    10321 ccgaatccgc cgtccccggc ggcctggcct ccgccctcgg ccccgtctcg gccgcggcgg
    10381 gggc
//
LOCUS       DAOOSJ010000069        10362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4186_length_10362_cov_5.968274, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000069 DAOOSJ010000000
VERSION     DAOOSJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4186_length_10362_cov_5.968274"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLG14_04960"
     CDS             <1..647
                     /locus_tag="PLG14_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOX48517.1"
                     /translation="DELGLSVTFMPKPMYKIAGSGMHVHQFVEKGGKSVFPGEGLYGL
                     SEVGLSYTAGLLEHALSGSLLAFSNPSTNSFRRLVAGYEAPVSATFAQGSRAAAVRIP
                     GYLKKGEARIEFRTGDATANTYYFLAAMLLAGLDGVQRKLDPVKKGFSTDKETPKNTF
                     PTGLYHVLAGLQKDKAYLSPVFPEELVSSWIGLKKKEAEYVYNAPVPQEYELYF"
     gene            762..2345
                     /locus_tag="PLG14_04965"
     CDS             762..2345
                     /locus_tag="PLG14_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX48518.1"
                     /translation="MGFETLMGDEAIALGALHAGLSAAFAYPGTPSTEIMEYYQDYAA
                     REGGIVAQWCANEKTAYESALGASYAGRRAMVSMKHVGLNVAADPFVNSALVKIKGGL
                     VVVVADDPGMHSSQDEQDSRALAEFARVPCLEPSDQQEAYDMVRRAFEISEAESCPVL
                     LRVTTRLAHSRAVVALGEKRAQNPVEKAPDRWGWTLMPHSARGLWKDLLSKYERLRLE
                     SEKEAVIEPGSSELGVITTGLAREYFLENLPEMAEKPTWLHIGRYPMPYGKIRELSGK
                     VKRVLVIEEGNPLVERQLRGILDQKVAVEGKESGQLPLTGELTPDSVRPALGLKPRKG
                     QACRDIVPPKRPPQLCQGCPHADSYAFLKEALAAYPRHSVNSDIGCYTLGAMAPYEAI
                     ETVVEMGASVGMARGAAEAGLTPAVAVIGDSTFYHSGLTNLLDAVSHNTNVKILVLDN
                     ETTGMTGAQPTILPGQRLKDIAVACGVDGAHVRILDAHKKDHAANVAAMKEELAYEGP
                     SVIIAVRACLEAVKKARKA"
     gene            2342..2914
                     /locus_tag="PLG14_04970"
     CDS             2342..2914
                     /locus_tag="PLG14_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOX48519.1"
                     /translation="MKYDIILCGVGGQGGISVSVVIAQAAMAEGLQVKQSEVHGMAQR
                     GGEVLAHLRLSDGPIASPMIPEGTARLVLAFEPLEALRYLPWLAPDAAVVAASAPIRN
                     IPNYPEIETVLAELRGLPNSLILDADALAREAGNVRAANMVLVGAAAKLLPLKTSSLE
                     KAIADTFARKGEAVVAANLKAFEHGRKANP"
     gene            2892..5201
                     /locus_tag="PLG14_04975"
     CDS             2892..5201
                     /locus_tag="PLG14_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOX48520.1"
                     /translation="MDEKLIRDIVARARAEGRDALTEVEGLGLLAAMGVEAPRHHFVK
                     GSAEAAALLADGAAAFPGERAVVKVISPEILHKTEVGGVAIVPNNAPSVAEAIAAMEA
                     KFTKERVDGYTVNEFVPYEPKIGHEMIVGYRFAKDFGPIVSFGPGGIYTEYLAKTFRE
                     GVANVFFSPATASRALVERELSRNAVRTLVAGGLRNTKPAIDEARIVDAVWAFIQASP
                     LLASAGVTEFEVNPFVVSRGRDGKGRLVALDVLVKLGEIAPASGKGELALGASGAVVN
                     RLQSIRPVGKIDRLLHPRSAAIVGVSEKGMNNGRIILRNLLENGFERSRLYVVKPGLA
                     EIDGCRCVPDIASLPEKVDLFVLVIPAAAAPGTIAELAELDKAESVIVIPGGLEEKSG
                     TEGIVARMREALERSRSGGSGGPLLNGGNCLGVRSVPGRYNTLFIPEYKLPMPKGEVA
                     PVALISQSGAFAICRVSKHADLNPKYLVTCGNQMDVTIGDYLERLAEDREIELFAVYV
                     EGFKPLDGEKTLRACKRIVDSGRSVVFYRAGRTAAGAAASASHTASIAGDYPVTKALM
                     AGVGAVVAESLDEFDDALTTFSLLRGKKASGRRLGAMSSAGFECVAFADNLGSLELAR
                     FSPETTPRLEKVFAKARISEIVDVHNPIDLTPMADDEAYEESFRAVLADPGVDCGVVG
                     IVPLSAMINTLAAGPAHKEDVTRDDSLAGRYGRLIKETEKPWVAVVDAGDLYDPLARE
                     LLSRGVPTFRSADRALKMLNLWMGARSKG"
     gene            5522..6238
                     /locus_tag="PLG14_04980"
     CDS             5522..6238
                     /locus_tag="PLG14_04980"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOX48521.1"
                     /translation="MKRLSAAIVLAAAAASAFSLGFGLKGGGPVRTEERRLSGFRSIE
                     MSGAGELRVKRGAAYRVAVTMDEDLLSSYESSVSGGVLRLGFRRGVAVTGLSRLEVEV
                     TLPEFEGLSLSGAAEARLLDAFSGRAFSFEASGSSALSGRIEYGRVELDLSGGCRLRL
                     EGRADSLDLGLSGASVVDAAGLAARTARVEASGASRIELRVQERLDVQTSGASSIRYY
                     GDPKLKVGSSGASTVTKAGD"
     gene            6514..7023
                     /locus_tag="PLG14_04985"
     CDS             6514..7023
                     /locus_tag="PLG14_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOX48522.1"
                     /translation="MLEPWFLWALLGLLLVGAETFIPGLAIIFFGLGALATALLCALP
                     AVAASPAAQAVFWTLSSLLSLVLLRKRFKRLLKGTIFYRQGEDEVDSLSGEAAVVLER
                     VSQDSPGRVRFRGTSWKAVAYLESFEPGETVRVVSREGLALVVTRDFLSPPGGGPAID
                     QAESPKEDR"
     gene            7023..8042
                     /locus_tag="PLG14_04990"
     CDS             7023..8042
                     /locus_tag="PLG14_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOX48523.1"
                     /translation="MAGIVFAYVVALAAIVALFKLIVLVPEQHAYVVELLGKYRKTLG
                     SGLHVLLPFVEKVAYRHQLKEDVIDVEPQVCITSDNVQVTVDGILYYKIFDPALASYG
                     IDDYRFATVQLAKTTMRSEIGKIELDRSFAEREAINDNIVRAVDEATDPWGIKVTRYE
                     VRSITPSEVITEAMERQMRAEREKRAEILASEGVKESRINVSKGERQEAINISMGERT
                     RTINQAEGKAKAIGIVSDATAEGLRLVAEALSSAGGKSAMALRLAEQYVDRLGQILGS
                     ASAQVLPEKLAEMRGLAEVLGLEGARLPLEPAPERSPDRGPDRGSPVGEKVRIGRPAR
                     EGGQS"
     gene            8039..8965
                     /locus_tag="PLG14_04995"
     CDS             8039..8965
                     /locus_tag="PLG14_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOX48524.1"
                     /translation="MNPLLPLYVLSGLFGLVLAIQLFRSIRIVPAQSVFIVERLGRFA
                     ASHGAGIHLLVPFLDRVAYKHNLKEQAVDVPSQECFTADNVKVHVDGVLYLRVVDPKR
                     ASYGVTNYRFASVCLAQTTIRAVMGKLELDRTFEERGAINASVVRAVDEAAAPWGVKV
                     TRYEIQNIKVPDSVLRSMELQMKAERDKRAEIAKSLGVMESKINYSQASMEEAVNKSE
                     GEKERAVNEAEGRASEILAVARATAESIKTLAAAVSVPNGEAAASLRVAESYIDALKR
                     LATPGTKVVLPLDLADPAAVTRHTDRLLGGRG"
     gene            8971..9843
                     /locus_tag="PLG14_05000"
     CDS             8971..9843
                     /locus_tag="PLG14_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOX48525.1"
                     /translation="MKRSKAALLVLLAAFLVLWALRFAYELSREEGRRPMPLGGFVQT
                     EAFDSSMAVQARKNYASAKIVIEQPQAPSQVYEQKYESVADLRSESPDFDGDSGRIKA
                     AAASVGAVVQRENSYGLPGSRVLSLSLGVVPESFDRAVEALKAIGELESITVTKTDRT
                     GDWKALEARRLSLEKTRDGLKALRANGGSLSDLVALETKILEIEGQIQELGVSLGDYS
                     EGASLCTVNLSLSEAKKAAAGLRVLRAAVDAFGWALLASLGAALACLAAAGAAALGAV
                     AVAKIRAMAAGRQA"
     gene            complement(9956..>10362)
                     /locus_tag="PLG14_05005"
     CDS             complement(9956..>10362)
                     /locus_tag="PLG14_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOX48526.1"
                     /translation="GTGGTISGLAAALKAKKPGLKAIAVEPLDSPVLSGGAPGPHKIQ
                     GIGAGFVPENFKRGLVDEILKARHEDSGAVARRLARTEGILVGISSGAALWAALELAK
                     RPEYAGKTIVVLLPDSGERYLSTWLFEEPQAT"
BASE COUNT         1560 a         3873 c         3468 g         1461 t
ORIGIN      
        1 ccgacgagct cggcctctcc gtcaccttca tgcccaagcc catgtacaag atcgcgggct
       61 ctggcatgca cgtccaccag ttcgtggaga agggcggcaa gtcggtcttc cccggcgagg
      121 gcctctacgg cctctccgag gtcggcctct cctacaccgc gggcctcctg gagcacgccc
      181 tctcgggctc cctcctggcc ttctccaacc cgagcacgaa ctccttccgc cgcctggtcg
      241 cgggctacga ggcgccggtc tccgcgacct tcgcccaggg ctcccgcgcc gccgcggtcc
      301 gcatccccgg ctacctcaag aagggcgagg cccgcatcga gttccgcacg ggcgacgcga
      361 cggcgaacac ctactacttc ctcgcggcga tgctcctcgc gggcctggac ggcgtccagc
      421 gcaagctcga cccggtgaag aagggcttct ccaccgacaa ggagaccccg aagaacacct
      481 tcccgaccgg cctctaccac gtcctggcag gcctccagaa ggacaaggcc tacctctccc
      541 ccgtcttccc cgaggagctc gtctcctcct ggatcgggct caagaagaag gaggccgagt
      601 acgtctacaa cgcgcccgtc ccccaggagt acgagctcta cttctaggcc tctcgcgaag
      661 tgcggaggcg gccccgcggc cgcctccgca ctggtccctc cgctttcccg gaccggcctg
      721 cgccggatct tcatttccac cacgctgtta aggaaggaat catggggttc gagactttga
      781 tgggtgacga ggcgatagcc ttgggagccc tgcacgcggg tctgtcggcc gccttcgcct
      841 accccggcac gccctccacc gagatcatgg agtactacca ggactacgcc gcccgcgagg
      901 gaggcatcgt cgcccagtgg tgcgccaacg agaagacggc ctacgagtcg gccctgggcg
      961 catcctacgc cggccgccgc gccatggtct ccatgaagca cgtcggcctc aacgtcgccg
     1021 ccgacccctt cgtcaactcc gccctcgtga agatcaaggg cggcctcgtg gtcgtcgtcg
     1081 ccgacgatcc cggcatgcat tcctcccagg acgagcagga cagccgcgcc ctggccgagt
     1141 tcgcccgcgt gccctgcctg gagccctccg accagcagga ggcctacgac atggtccgcc
     1201 gggccttcga gatttcggag gccgaatcct gccccgtcct cctccgcgtg acgacccgcc
     1261 tggcgcacag ccgcgcggtc gtggccctgg gcgagaagcg ggcccagaac cccgtggaga
     1321 aggcccccga ccgctggggc tggaccctca tgccccactc cgcgcgcggc ctctggaagg
     1381 acctcctttc gaagtacgag cgcctccgcc tcgagtccga gaaggaggcc gtcatcgagc
     1441 ccggctcctc cgagctcggc gtaatcacga cgggcctcgc ccgcgagtac ttcctggaga
     1501 acctccccga gatggcggag aagccgacct ggctccacat cggccgctat ccgatgccct
     1561 acgggaagat ccgcgagctc tccggcaagg tcaagcgcgt cctcgtcatc gaggagggca
     1621 accctctcgt ggagcgccag ctccgcggaa tcctggacca gaaggtcgcc gtcgagggca
     1681 aggagtcggg ccagctcccc ctcacgggcg agctcacgcc ggacagcgtc cgcccggccc
     1741 tcggcctcaa gcccaggaag ggccaggcct gccgggacat cgtcccgccc aagcgcccgc
     1801 cccagctctg ccagggctgc ccccacgccg actcctacgc cttcctgaag gaggccctgg
     1861 cggcctaccc gcggcactcc gtgaactccg acatcggctg ctacaccctg ggcgcgatgg
     1921 cgccctacga ggccatcgag acggtcgtcg agatgggcgc gagcgtcggc atggcgcgcg
     1981 gcgccgcgga ggcggggctc acgcccgcgg tcgccgtcat cggcgactcc accttctacc
     2041 actcgggcct cacgaacctc ctggacgcgg tgtcgcacaa cacgaacgtg aagatcctgg
     2101 tcctggacaa cgagaccacc ggcatgacgg gcgcccagcc caccatcctg cccggccagc
     2161 gcctcaagga catcgcggtc gcctgcggcg tcgacggcgc ccacgtccgg atcctggacg
     2221 cgcacaagaa ggaccacgcc gcgaacgtgg cggccatgaa agaggagctc gcctacgagg
     2281 gcccgtccgt gatcatcgcg gtccgcgcct gcctcgaggc cgtgaagaaa gcgaggaagg
     2341 catgaagtac gacatcatcc tctgcggcgt cggcggccag ggcggcatct ccgtctccgt
     2401 cgtgatcgcc caggccgcga tggccgaggg cctccaggtc aagcagtccg aggtccacgg
     2461 catggcccag cgcggcggcg aggtcctggc ccacctgcgc ctctccgacg gccccatcgc
     2521 gagccccatg atccccgagg gcaccgcccg cctggtcctg gccttcgagc ccctggaggc
     2581 cctccgctac ctgccctggc tcgcgcccga cgccgccgtc gtggcggcct ccgccccgat
     2641 caggaacatc ccgaactacc ccgagatcga gaccgtgctc gcggagctcc ggggcctccc
     2701 gaactccctc atcctggacg ccgacgccct ggcccgcgag gccggcaacg tccgggccgc
     2761 gaacatggtc ctggtcggcg ccgccgcgaa gctcctcccc ctcaagacct cgagtctgga
     2821 gaaggccatc gccgacacct tcgcccgcaa gggcgaggcc gtcgtggccg cgaacctgaa
     2881 ggcattcgag catggacgaa aagctaatcc gtgacatcgt cgcccgcgcg cgggcggagg
     2941 ggcgggacgc cctgaccgag gtcgagggcc tgggcctcct cgccgccatg ggcgtcgagg
     3001 ccccgcgcca ccatttcgtg aagggctccg ccgaggcggc ggccctcctc gcggacggcg
     3061 cggccgcctt ccccggggag cgggccgtcg tcaaggtgat cagcccggag atcctccaca
     3121 agaccgaggt cggcggcgtc gccatcgtgc cgaacaacgc gccctccgtc gccgaggcga
     3181 tcgccgccat ggaggccaag ttcacgaagg agcgcgtgga cggctacacg gtgaacgagt
     3241 tcgtccccta cgagcccaag atcggccacg agatgatcgt cggctaccgc ttcgccaagg
     3301 acttcggccc gatcgtgtcc ttcggcccgg gcggcatcta taccgagtac ctcgccaaga
     3361 ccttccggga gggcgtcgcg aacgtcttct tctcccccgc cacggccagc cgcgccctcg
     3421 tggagcgcga gctgtcgcgc aacgcggtcc gcaccctggt cgccgggggc ctcaggaaca
     3481 cgaagcccgc gatcgacgag gcgaggatcg tggacgctgt gtgggccttc atccaggcct
     3541 cccccctcct cgcctccgcc ggcgtgacgg agttcgaggt gaaccccttc gtcgtgtccc
     3601 gcggccgcga cgggaagggc cggctcgtcg ccctggacgt cctcgtgaag ctcggggaga
     3661 tcgcccccgc ctccgggaag ggcgagctcg ccctcggagc ctccggcgcg gtcgtgaacc
     3721 gcctccagtc gatccggccc gtcggcaaga tcgaccgcct cctccatccc cggagcgccg
     3781 cgatcgtcgg cgtctcggag aaggggatga acaacggccg catcatcctc cgcaacctcc
     3841 tggagaacgg cttcgagagg agccgcctct acgtcgtgaa gcccggcctc gcggagatcg
     3901 acggctgccg ctgcgtgccg gacatcgcct ccctcccgga gaaggtggac ctcttcgtcc
     3961 tcgtcatccc cgccgccgcc gcgcctggca cgatcgccga gctcgccgag ctcgacaagg
     4021 cggagtcggt catcgtcatc cccggaggcc tcgaggagaa gtcgggcacc gagggcatcg
     4081 tcgcgcgcat gcgcgaggcc ctggagcgct cgcgctccgg cggctcgggc ggccccctcc
     4141 tcaacggcgg gaactgcctg ggcgtccgct ccgtgcccgg caggtacaat accctcttca
     4201 tccccgagta caagctcccg atgcccaagg gcgaggtcgc gcccgtcgcc ctcatctccc
     4261 agtcgggcgc cttcgcgatc tgccgcgtct ccaagcacgc ggacctgaac ccgaagtacc
     4321 tcgtcacctg cggcaaccag atggacgtca cgatcggcga ctacctggag cgcctggcgg
     4381 aggaccgcga gatcgagctt ttcgccgtct acgtggaggg cttcaagccc ctggacggcg
     4441 agaagaccct cagggcctgc aagcgcatcg tcgactccgg ccggagcgtc gtcttctacc
     4501 gcgcgggccg caccgccgcg ggcgccgcgg cgagcgcgag ccacacggcg agcatcgcgg
     4561 gcgactaccc ggtcaccaag gccctgatgg cgggcgtcgg cgcggtcgtc gcggagagcc
     4621 tggacgagtt cgacgacgcg ctcacgacct tctccctcct ccgggggaag aaggcctcag
     4681 gccgccgcct gggcgccatg tccagcgccg gcttcgagtg cgtcgccttc gcggacaacc
     4741 tgggctccct ggagctcgcc cgcttctcgc ccgagaccac gccgcgcctg gagaaggtct
     4801 tcgcgaaggc gaggatcagc gagatcgtcg acgtgcacaa cccgatcgac ctcacgccga
     4861 tggccgacga cgaggcctac gaggagagct tccgcgccgt cctcgccgac ccgggcgtcg
     4921 actgcggcgt cgtgggcatc gtgcccctct ccgcgatgat caacaccctc gcggccggcc
     4981 ccgcccacaa ggaggacgtc acgcgcgacg actcgctcgc cgggcgctac ggcaggctca
     5041 tcaaggagac ggagaagccc tgggtcgcgg tggtcgacgc gggcgacctc tacgatcctc
     5101 ttgcccgcga gctcctctcc cggggcgtgc cgactttcag gagcgcggac cgcgccctca
     5161 agatgctcaa cctctggatg ggcgcccgct cgaagggcta aaggcaggcg gcgggccttt
     5221 catggcccgc catcggcccg cctgcccttc cggcggcccg cgcccggttc ccgccggcag
     5281 cctcccggcc gccgcctcgt gatgacccga accagccgcc cctagacggg ggcggctttg
     5341 tcttttctgt ggaaacgcgc tattatatgc cggtatccgg acggcacagt tgtccgcgcg
     5401 cccaagtctt tggggcgcgg gcccgccggg gccggccgcg gcggccctgc aagggcgccc
     5461 ggcgcaagac cttcagcgac aggccgccga ggtacgctcg cttttcgcaa ggagcgcgta
     5521 gatgaaacga ctttccgccg ccatcgtcct ggccgccgcc gcggcctccg ccttctccct
     5581 cggcttcggc ctcaagggag gcggtcccgt ccgcacggag gagaggaggc tctcgggctt
     5641 ccgctccata gagatgtcag gcgccgggga actccgcgtc aagcgcggcg ccgcctaccg
     5701 cgtcgcggtc accatggacg aggacctcct ctcctcctac gagtcgtcgg tctcgggcgg
     5761 cgtcctccgc ctgggcttca ggcgcggcgt cgcggtgacc ggcctctccc gcctggaagt
     5821 cgaagtcacg ttgcccgaat tcgagggcct gtccctctcg ggcgccgccg aggcccgcct
     5881 cctcgacgcc ttctccggcc gcgccttctc cttcgaggcc tcgggctcga gcgccctctc
     5941 gggcaggatc gagtacggcc gcgtcgagct cgacctttcg ggcggctgca ggctcaggct
     6001 cgagggcagg gcggacagcc tggacctcgg cctctccggc gcctccgtcg tcgacgcggc
     6061 cggactcgcc gccaggacgg ccagggtcga agcctcgggg gcgagcagga tcgagctccg
     6121 cgtccaggag cgcctggacg tccagacgag cggcgcctcc tccatccgct actacggcga
     6181 tcccaagctg aaggtgggct cgagcggcgc ctccaccgtg acgaaggccg gggactaggc
     6241 cgccggcccg ccgcgctggc ggacctctcc gacgcaggcc gcggcgccgc cgcatagact
     6301 atcgcggcgc cgctacctgc gaggtcccgc ggcgaccgcc atggcggcgc cgccgcgttg
     6361 cctgtcgcgg cgcggcgagc cgcgcggccc cgccccgcca gccatcgcgg cgcggctcct
     6421 cgcttgaccg cgggaaccgc gccgcctatc ctgagtctgg ggcgagaggg ccggcgcgag
     6481 gccggccgcc gctcccggaa ggcgcgaaaa gcgatgctag aaccctggtt cctgtgggcc
     6541 ctcctcggcc tcctcctagt cggggccgag accttcatcc cgggcctcgc gatcatcttc
     6601 ttcggcctgg gcgccctggc gacggccctg ctctgcgccc tgccggccgt cgcggcgagc
     6661 ccggcggccc aggccgtctt ctggaccctc tccagcctcc tctccctcgt cctcctcagg
     6721 aagcgcttca agcggctcct caaggggacg atcttctacc gccaaggcga ggacgaggtg
     6781 gacagcctct ccggcgaggc ggccgtcgtc ctggagcgcg tgtcgcagga cagccccggc
     6841 cgcgtgcgct tccgcggcac gagctggaag gccgtcgcct acctcgagtc cttcgagccg
     6901 ggggagaccg tccgcgtcgt ctcccgcgag ggcctggccc tcgtcgtgac ccgcgatttc
     6961 ctctcccctc cgggcggcgg cccggcgata gaccaagcag aaagccccaa ggaggatcgg
     7021 tgatggcggg aatcgtcttc gcctacgtcg tcgcgctcgc ggcgatcgtg gcgctcttca
     7081 agctgatagt cctcgtgccc gagcagcacg cctacgtcgt ggagctcctc gggaagtacc
     7141 ggaagacgct gggctccggc ctccatgtcc tcctgccctt cgtggagaag gtggcctacc
     7201 gccaccagct caaggaggac gtgatcgacg tcgagccgca ggtctgcatc acgtcggaca
     7261 acgtccaggt gacggtggac ggcatcctct actacaagat cttcgacccg gccctggcga
     7321 gctacggcat cgacgactac cgcttcgcga cggtgcagct cgcgaagacg acgatgcgct
     7381 cggagatcgg caagatcgaa ctggaccgga gcttcgccga gcgcgaggcg atcaacgaca
     7441 acatcgtgcg cgcggtcgac gaggccaccg atccctgggg catcaaggtc acgcgctacg
     7501 aggtccgctc gatcacgccc agcgaggtca tcaccgaggc gatggagcgg cagatgcgcg
     7561 ccgagcgcga gaagcgcgcg gagatcctgg ccagcgaggg cgtcaaggag agccgcatca
     7621 acgtctccaa gggcgagcgg caggaggcca tcaacatctc gatgggcgag cgcacgcgga
     7681 ccatcaacca ggcggagggc aaggcgaagg cgatcggcat cgtgagcgac gcgaccgcgg
     7741 agggcctgag gctcgtcgcg gaggccctct cctccgcggg cggcaagtcg gcgatggccc
     7801 tgcgcctggc cgagcagtac gtggacaggc tcggccagat cctgggctcc gcgagcgccc
     7861 aggtcctgcc cgagaagctc gcggagatgc gcggcctggc cgaggtcctg ggcctcgagg
     7921 gcgcgcgcct cccgctcgag cccgcgccgg agcggagccc cgacaggggc cccgacaggg
     7981 gaagccccgt cggcgagaag gtcaggatcg ggcgccccgc ccgcgaagga ggccagtcgt
     8041 gaacccgctt ctcccgctct acgtcctctc cggactcttc ggcctcgtgc tcgccatcca
     8101 gctcttccgc tcgatccgca tcgtgcccgc gcagtcggtc ttcatcgtcg agcgcctggg
     8161 gcgcttcgcc gcgagccacg gcgcgggcat ccacctcctc gtacccttcc tggaccgcgt
     8221 cgcctacaag cacaacctga aggagcaggc cgtcgacgtg ccctcccagg agtgcttcac
     8281 cgccgacaac gtgaaggtcc acgtggacgg cgtcctctac ctccgcgtcg tggaccccaa
     8341 gcgcgcgagc tacggcgtca cgaactaccg cttcgccagc gtctgcctcg cgcagacgac
     8401 gatccgggcg gtgatgggca agctcgagct cgaccggacc ttcgaggagc gcggagcgat
     8461 caacgcctcc gtcgtgcgcg cggtggacga ggccgccgct ccctggggcg tcaaggtcac
     8521 ccgctacgag atccagaaca tcaaggtgcc cgactccgtg ctgcgctcga tggagctgca
     8581 gatgaaggcc gagcgcgaca agcgcgcgga aatcgcgaag agcctgggcg tgatggagag
     8641 caagatcaac tactcccagg cctccatgga ggaggccgtg aacaagagcg agggcgagaa
     8701 ggagcgcgcc gtcaacgagg ccgagggccg cgcctccgag atcctcgccg tggcccgcgc
     8761 cacggccgag tcgatcaaga ccctggccgc ggccgtgtcg gtgcccaacg gcgaggcggc
     8821 cgcctccctg cgggtcgccg agtcctacat cgacgcattg aagcggctcg cgacgcccgg
     8881 caccaaggtg gtcctgcccc tcgacctggc cgaccccgcc gcggtgacgc ggcacacgga
     8941 caggctcctg ggcgggcggg gatgagggcg atgaagcgga gcaaggccgc gctcctcgtc
     9001 cttctcgccg ccttcctggt cctctgggcc ctgcgcttcg cctacgagct ctcgcgcgag
     9061 gaaggacggc ggcccatgcc cctcgggggc ttcgtccaga cggaggcctt cgactcctcc
     9121 atggccgtcc aggcgaggaa gaactacgcg agcgcgaaga tcgtcatcga gcagccgcag
     9181 gccccgagcc aggtctacga gcagaagtac gagagcgtcg cggacctccg ctcggagagc
     9241 ccggacttcg acggggactc ggggcggatc aaggcggcgg cggcctccgt cggcgccgtc
     9301 gtgcagcgcg agaattccta cggcctcccg ggctcgcggg tcctctccct gagcctcggg
     9361 gtcgtgccgg agtccttcga ccgcgcggtc gaggctctca aggcgatagg cgaactcgag
     9421 tcgatcaccg tgacgaagac cgacaggacc ggcgactgga aggccctgga ggcgcgccgc
     9481 ctctccctgg agaagacccg cgacggcctc aaggccctcc gcgcgaacgg cggctccctc
     9541 tccgacctgg tcgccctgga gacgaagatc ctggagatcg aggggcagat ccaggagctc
     9601 ggcgtctccc tgggggacta cagcgagggc gcctccctct gcaccgtgaa cctctcctta
     9661 agcgaggcga agaaggcggc cgcgggcctc cgcgtcctca gggccgcggt cgacgcgttc
     9721 ggctgggccc tcctcgcgag cctgggcgcc gccctggcct gcctcgcggc ggccggcgcg
     9781 gcggccctgg gtgcggtcgc cgtcgccaag atcagggcga tggcggcggg aaggcaggcc
     9841 tgaggcccgg ccctcccctg gaaagcgggg cgccccggaa tcccggggcg ccttttttgt
     9901 ccgcggccgc atttcgccgg cccgccggcg cggatcccgg cccgccgggc cgggctcagg
     9961 tggcctgcgg ctcctcgaag agccaggtgg agaggtagcg ctccccggag tcggggagaa
    10021 ggacgacgat ggtcttgccc gcgtactcgg gccgcttcgc gagctccagg gcggcccaga
    10081 gggcggcgcc cgaggagata ccgaccagga tgccctccgt cctggccagg cggcgggcga
    10141 cggcgcccga gtcctcgtgg cgggccttca ggatctcgtc gacgaggccc cgcttgaagt
    10201 tctccggcac gaagcccgcg ccgatcccct ggatcttgtg gggacccggc gcgccgcccg
    10261 agaggacggg ggagtcgagg ggctcgacgg ctatggcctt gaggcccggc ttcttcgcct
    10321 tgagggcggc ggccaggccg gagatcgtgc ctcccgtgcc ga
//
LOCUS       DAOOSJ010000070        10357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4190_length_10357_cov_4.658125, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000070 DAOOSJ010000000
VERSION     DAOOSJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_10357_cov_4.658125"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="PLG14_05010"
     CDS             <1..1688
                     /locus_tag="PLG14_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX48527.1"
                     /translation="KPGFEDKVFYVWFDAPIGYVSISACAGDEKGFDWKSWWQSPEDV
                     ELFQFIGKDNIPFHTVIFPSSLLGSGGKWTMLHHMSSSEYLNYESGKFSKSKGVGVFG
                     TDAVESGIPADVWRFYIFWNRPEKSDYTFTWSDFQEKVNGELIGNLGNLANRTLTFVA
                     RYYDGKIPEGRADEAFWAKAREIEAGIAAKLERAELRDAFREAFELSNLANKRFQDGE
                     PWKTRTSDPEAAASLIRDLCYVLRDLAVIVHPYMPRAAERLAGFLGKSLKAEGVEAAA
                     GGLSWADVGVLEGLSRVEKPEVLFAKLEDELVASLRDRYSGSQKERAERGVASGAAAT
                     GAAATGAAATGAAASGAAQGKPAAAKPAAVKAPEAAAKGSEAAAKPAPEPKPVPYEGL
                     PAEERFAKLIDLRVAKIVKIERHPKADKLYIETLDDGSGTERVIVSGLVPFYKEEELL
                     GKHIVLANNLKPAKLRGVESRGMLLAASRKSEGEGVKEAVEVLDAGGAAPGTRVVLEG
                     QDPAVPADAEIDVDAFFSVAIRAEGGAAMVGGKRLLADGKPFALARVSEGEVG"
     gene            1748..2986
                     /locus_tag="PLG14_05015"
     CDS             1748..2986
                     /locus_tag="PLG14_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOX48528.1"
                     /translation="MTVLLEPAPKAACDGSASFLQSGFWGDFKAGFGWKPLHFTLESG
                     GRRSPLLVLLRGLGKGLSFAYVPHGPALEPGPGEAAALLAGIGEALKDRLPATCLFVR
                     FDPPWRLIEATADDGGADDGDRAGAAKAATPLFGQAAEGQGSPLPGQPAGGQGSRRPR
                     EPQRPPVGAPLRRAAADIQPPDTVLLDLSRSEEDILQGMKPKWRYNVRLAEKKGVAVE
                     ELAGEAALASLPEFYRLYVETSERDRIALHPEAYYAGLFSLSRTYDPEGGRRPDFRLW
                     MARHEGEALAAIITVFRGEEAVYLYGASSGEKRNLMPAYALQWAAIRAAKASGAKVYD
                     FYGIPPVDDPSHPMSGLYRFKTGFGGEIAHRAGSWDYPLRPVAYAAFRAAEAARTWWF
                     KDFRKRAGGRGERGAAKRTE"
     gene            complement(3002..3589)
                     /gene="amrA"
                     /locus_tag="PLG14_05020"
     CDS             complement(3002..3589)
                     /gene="amrA"
                     /locus_tag="PLG14_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOX48529.1"
                     /translation="MSPAPGTGPGGGLGEAEKAELLAYARAVIGAALGDSLAVLPADP
                     ERFGRDCGAFVSLHERGSLRGCIGRMSAEGLLVHTVAEMARAAAFEDPRFPRLRREEL
                     PEVAIEITVLSPMRAIDDPKEIEIGRHGVHLSLAGRSAVFLPQVAPEQGWGREELLDN
                     LALKAGLPPASWNRSEARLSVFEGLVFGEAGGTRA"
     gene            complement(3586..4473)
                     /gene="amrB"
                     /locus_tag="PLG14_05025"
     CDS             complement(3586..4473)
                     /gene="amrB"
                     /locus_tag="PLG14_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOX48530.1"
                     /translation="MIESSEKRKRREATVAGIFYPEEPDALGAEIDRFLGSAEPLFGG
                     RIVSRARAILAPHASLGYSGDLAALAWKAAEGRGVSRVVAISPLHRAEESLVYLPEAE
                     FFETPFGPVPVDQRSVEELLDCGTAFSRNDIPHFEEHGVEMQLPFMRRLFPRASLVPI
                     LVGKPSSSLVRALASALGIVFAKEAEETLFVLSTDLATCAKSESDAERAEAEDRAERF
                     IELVAQGDWRGILEERAGSEYAACGAACVAAWLASPLAEDSRSLVLGMHDSSASRESE
                     QERLVFYAALAFAAGDGEA"
     gene            4538..5926
                     /locus_tag="PLG14_05030"
     CDS             4538..5926
                     /locus_tag="PLG14_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX48531.1"
                     /translation="MRVLIVDDERRMTESLAEYLGLEGIEIKAAGDGAEAKALLCEEA
                     FDALVTDLRMPKLDGLGLLSWLREEGPSVPVIVISAHGEVRDAVAAMRLGAYDYLVKP
                     FDPEELVIRLNKAVAERRASGRLSAGERRYEEGTRLIGESKPMREVLALVERAAPSNA
                     NILLTGESGTGKEVAARLVHELSGREGPFVPVNVGAVPESLLESELFGSEKGAFTGAD
                     ARRTGLFETAQGGTIFLDEMGELPLHLQVKLLRAIQEKKIMRLGGVKPIPVDARLVAA
                     TNRDLEAEVAAGRFREDLYYRLNVIRIRLPPLRERSGDIGLLAGYLLGKLQAELGRRV
                     EGMSAEAIALLESYSFPGNVRELENALERALILADGPRIEARDLRLGSGGEALARGQG
                     SEAKAGGPCEPFAEPRSLAEIEKAAIEASLRRNRFRRERTAEELGISRRTLLNKVKEY
                     GIAVPGRDDEED"
     gene            5931..6818
                     /locus_tag="PLG14_05035"
     CDS             5931..6818
                     /locus_tag="PLG14_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOX48532.1"
                     /translation="MGERKATRSFRGRRTQDGAGVSLTRIFGFGNEEELDPFLLLDAF
                     GSKDPREYLPGFPWHPHRGIETVTYLLEGSVRHGDSLGNAGVIGPGELQWMTAGSGII
                     HEEMPLASPGGVLGFQLWVNLPRAEKMREPAYRGVLAGEVPRIRVPGGEVGVLAGDFG
                     GFRGPVEGVARNPAYFELRLEPGASLSLEAPSEETAFIYVFEGSLAEAGAGDRASAGD
                     RACAVAGDCLLFAAPGEDDKISVASGAEGARLIFARARPLREPIAWRGPIVMNSEEEL
                     DLAFSEFHAGTFVKAKRSR"
     gene            complement(6796..7347)
                     /locus_tag="PLG14_05040"
     CDS             complement(6796..7347)
                     /locus_tag="PLG14_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOX48533.1"
                     /translation="MLKRLAELSGGRILVETGDITDFEGDAIVNAANSGLLGGGGVDG
                     AIHRAAGPELLEACRKIRAERLPGGLPAGKAVLTGGYRLRARAVIHTVGPVYEGGGRG
                     EAEVLASAYRSSLELASREGFKEIAFPAISTGVYGYPKAEAAAVALGVVSAFLASHAL
                     PEQVHLVFYGEGDARAFIDSVSP"
     gene            complement(7475..8650)
                     /locus_tag="PLG14_05045"
     CDS             complement(7475..8650)
                     /locus_tag="PLG14_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48534.1"
                     /translation="MLEIRGLRFSRPGFSLDLDLELPEGAYGVLLGASGCGKSTALRL
                     VAGLLEPEGGSVRLAGRELLGLAPERRRVGMVFQDYALFPQLSVRRNIEYGPRLRGAA
                     SEERRKLSQELGKAFRIEGLLERRPASLSGGEQQRVALARSVAARPELLLLDEPLSSL
                     DAALRRALRGEIRERIRDAGLSALHVTHDLEEALAIADRLFIMDGGRVVEAGDPEAIY
                     ARPGSAFAARFLGRGPLIPVTRIIESGPRPLVLTPFGKLRASRGLPTDPAASETESCT
                     SGASGTELPKGSLAEGRPANGSPTGGQCYLHFGAGEARALPGSSAASIAADADGVYNE
                     LSGVAGESYFLGTTRRVSLRPPAGGEPIELELGPEIRPSRGEALVLVLPIDSCILLR"
     gene            complement(8643..10193)
                     /locus_tag="PLG14_05050"
     CDS             complement(8643..10193)
                     /locus_tag="PLG14_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX48535.1"
                     /translation="MGLLKSLGAALSSPSTLRALDFGLKEAGASALLALAIGLPGAYL
                     VARHEFPGRRLLKALAGLPFCVPPILVVLAFVLYFGRNGYLNRFLMAAFGLAEPPLGF
                     LYSFWGLVLVHGFYNFPIVLQTVGDAWENVPRDREEAARTLGAGRLRAFATGILPSLL
                     PVILQASALVFLFCFFSFVVVLVFGPLGGSTLEVEIFRAARMSADPAGAAALSLIETA
                     AALLVVLLLTAAERRGGEAAKKTGTRQPRSKAVGPAFVFLVVYAVFLLVFFAGPLAAL
                     LAEAFTERRGMGGGGRLGFGNFARLLFGGPQRGAETGGVNWMAPQGPLLGALGDSLAT
                     ALPAAALATVLGGVAALLLRGKRSRAMETALSLPLAVSGIVLALGWSFLFPRANRLLV
                     VLVEAMAALPFVLRSLMAALAAVDRTPLLAARTLGAGRLRAALDIEARSILPAFLTAS
                     AFAFSIAAGDASAPLLIGAPGFEPLPLLVYRLVGSYRFPEACAAGIVLALVTGLVFFA
                     KDKDALHA"
BASE COUNT         1658 a         3598 c         3678 g         1423 t
ORIGIN      
        1 ccaagcccgg cttcgaggac aaggtcttct acgtctggtt cgacgcgccc atcggctacg
       61 tctccatctc cgcctgcgcc ggcgacgaga agggcttcga ctggaagtcc tggtggcaga
      121 gccccgagga cgtcgagctc ttccagttca tcggcaagga caacatcccc ttccacacgg
      181 tgatcttccc ctcgagcctc ctcggctccg gcgggaagtg gacgatgctc caccacatgt
      241 cctcgagcga gtacctgaac tacgagtccg gcaagttctc caagtccaag ggcgtcggcg
      301 tcttcggcac cgacgcggtc gagtccggca tccccgccga cgtctggcgc ttctacatct
      361 tctggaaccg gccggagaag tccgactaca ccttcacctg gagcgacttc caggagaagg
      421 tgaacggcga gctcatcggc aacctgggca acctggcgaa ccgcaccctc accttcgtcg
      481 cgcgctacta cgacgggaag atccccgagg gcagggccga cgaggcgttc tgggccaagg
      541 cccgcgagat cgaggcgggc atcgcggcca agctcgagcg cgccgagctc agggacgcct
      601 tccgcgaagc attcgagctt tcgaacctcg cgaacaagcg cttccaggac ggcgagccct
      661 ggaagacgag gacgagcgac cccgaggccg ccgcctccct catccgcgac ctctgctacg
      721 tcctccgcga cctcgccgtc atcgtccatc cctacatgcc gcgggccgcc gagcgcctcg
      781 cgggcttcct cgggaagagc ctcaaggcgg aaggggtcga ggcggccgcg ggcggcctct
      841 cctgggccga cgtcggcgtc ctcgagggcc tctcacgcgt cgagaagccc gaggtcctct
      901 tcgcgaagct cgaggacgag ctcgtcgcct ccttgcgcga ccgctactcg ggctcgcaga
      961 aggagcgggc ggagcgcggc gtggcctctg gcgccgccgc gaccggcgcc gccgcgaccg
     1021 gcgccgccgc gaccggcgcc gccgcttccg gcgccgccca gggcaagccg gccgcggcca
     1081 agcccgccgc cgtgaaggcc cccgaggccg ccgccaaggg ctccgaagcc gcggcgaagc
     1141 ccgcgcccga gcccaagccc gtcccctacg agggcctccc cgcggaggag cgcttcgcga
     1201 agctcatcga cctccgcgtc gccaagatcg tgaagatcga gcgccatccc aaggccgaca
     1261 agctctacat cgagaccctg gacgacggct cggggaccga gcgcgtcatc gtctccggcc
     1321 tcgtcccctt ctacaaggag gaggagctcc tcggcaagca catcgtcctc gcgaacaacc
     1381 tgaagcccgc gaagctccgc ggcgtcgaga gccgcggcat gctcctggcc gcctccagga
     1441 agtcggaggg cgagggcgtc aaggaggccg tggaggtcct ggacgcgggc ggcgccgcgc
     1501 ccggcaccag ggtcgtcctc gaggggcagg atcccgccgt tcccgccgac gcggagatcg
     1561 acgtcgacgc cttcttctcc gtcgccatcc gcgcggaggg cggggccgcc atggtgggcg
     1621 gcaagcgcct cctcgccgac ggcaagccct tcgccctggc ccgcgtgagc gagggcgagg
     1681 taggctgagc ccggcgccgg cggacctggg ctccccgccc cgccggcgcc gcgcgatgcg
     1741 cgacaccatg accgtacttc tagaaccggc tccgaaggcc gcctgcgacg gctcggcgag
     1801 cttcctccag tcgggcttct ggggcgactt caaggcgggc ttcggctgga agcccctcca
     1861 ctttacgctc gagtcgggcg ggaggcgctc gcccctcctc gtcctcctcc gcggcctggg
     1921 gaagggcctc tccttcgcct acgtccccca cggccccgct ttggagcccg ggcctggaga
     1981 agccgccgcc ctcctggccg gcatcgggga ggccctgaaa gaccgcctcc cagccacctg
     2041 cctctttgtc cgcttcgacc cgccctggcg cctcatcgag gccacggccg acgacggcgg
     2101 cgcggacgac ggggacagag ccggcgcggc gaaggcagct acgccgctgt tcggccaggc
     2161 cgccgaggga cagggctcgc ccttgccagg ccagcccgcc gggggacagg gctcgcggcg
     2221 gcccagggag cctcagcggc cgcccgtcgg agcccccttg aggcgcgccg cggcggacat
     2281 acagcccccc gacacggtcc tcctcgacct ctcgaggagc gaagaggaca tcctgcaggg
     2341 catgaagccc aagtggcgct acaacgtgcg cctggcggag aagaagggcg tcgcggtcga
     2401 agagctcgcc ggcgaggctg ccctcgcttc cctgccggaa ttctaccgcc tctacgtgga
     2461 gacctcggag cgcgaccgga tcgcgctgca tccggaggcc tattacgccg gcctcttctc
     2521 cctctcgcgg acctatgatc ccgagggcgg ccggcgtccc gacttccgcc tctggatggc
     2581 caggcacgag ggggaagccc tcgcggctat catcaccgtc ttccgcgggg aggaggcggt
     2641 ctacctctac ggcgcctcct cgggggaaaa gcggaacctc atgcccgcct acgccctaca
     2701 gtgggccgcg atccgcgccg cgaaggcctc gggagcgaag gtctacgatt tctacggcat
     2761 cccgcccgtc gacgacccga gccaccccat gtcgggcctc taccgcttca agaccggctt
     2821 cgggggcgag atcgcccatc gcgcaggctc ctgggactac cccctgcggc ccgtcgccta
     2881 cgcggccttc cgcgccgcgg aggcggccag gacctggtgg ttcaaggatt tcaggaagag
     2941 agcaggcggg cgcggagagc gcggcgcggc caagcggacc gaataggctc gcgcagggcg
     3001 cttaggccct cgtcccgccc gcctcgccga agaccagccc ctcgaagacg gagagcctgg
     3061 cctcggagcg gttccaggag gcgggcggca gcccggcctt gagggctagg ttgtccagga
     3121 gctcctccct tccccagccc tgctccggag cgacctgggg caggaagacg gcgctacggc
     3181 cggcgagcga gaggtggacc ccgtggcggc caatctcgat ctccttcgga tcgtcgatgg
     3241 cccgcatcgg cgagagcacg gtgatctcga tggcgacttc cggaagctcc tcgcgcctga
     3301 gccgcgggaa gcgagggtcc tcgaaggcgg cggcccgcgc catttcggcc accgtatgca
     3361 cgaggaggcc ctcggccgac atccttccga tgcagccgcg gagcgagcct cgctcgtgga
     3421 gggagacgaa ggcgccgcag tccctgccga agcgttcggg gtcggcgggg aggacggcga
     3481 gggagtcgcc cagggccgcg ccgatcaccg ccctcgcgta ggcgaggagc tcggcttttt
     3541 ccgcttcgcc caggccgccg ccggggccgg ttccgggcgc ggggctcatg cttccccgtc
     3601 ccccgccgcg aaggccaggg ccgcgtagaa gacgagccgc tcctgctccg actccctcga
     3661 ggcgctcgag tcgtgcatgc ccaggacgag ggaccttgag tcctccgcta gaggggaggc
     3721 gagccaggcg gcgacgcagg cggccccgca ggccgcgtac tcgctccccg ccctctcctc
     3781 caggatgcct ctccagtcgc cctgcgcgac gagctcgatg aatcgctcgg ccctgtcctc
     3841 ggcctccgcc cgctcggcgt cgctctcgga cttggcgcag gtcgcgaggt cggtcgagag
     3901 gacgaagagc gtctcctccg cctccttggc gaacacgatg ccgagggcgg aggcgagggc
     3961 gcggacgagg gaggaggagg gcttcccgac caggatcggc acgagggagg cgcgggggaa
     4021 gaggcggcgc atgaagggca gctgcatctc gacgccgtgt tcctcgaagt gcgggatgtc
     4081 gttgcgggag aaggccgtgc cgcagtccag gagctcctcg acggagcgct ggtccacggg
     4141 cacgggaccg aagggcgtct cgaagaactc cgcctcggga aggtagacca gggactcctc
     4201 ggcgcggtgg agaggggaga tggcgacgac gcgggagacg cccctgccct cggcggcctt
     4261 ccaggccagg gcggccaggt cgcccgagta gccgaggctc gcgtggggcg cgaggatcgc
     4321 gcgcgcccgg gagacgatgc ggcccccgaa gaggggctcg gcggagccga ggaagcggtc
     4381 gatctcggcg ccgagggcgt ccggctcctc ggggtagaaa atgcccgcga ccgtcgcttc
     4441 cctccgcttc ctcttttcgc tactctcgat catagtcgta agtatagtct tcggcccggg
     4501 gctttcaagc gccggcgccc tgcgaggagc gtaagtcttg cgcgtgctca ttgtcgacga
     4561 cgagcggagg atgaccgagt ccctcgccga atacctcggc ctcgagggca tcgagatcaa
     4621 ggcggccggc gacggggccg aggccaaggc gctgctttgc gaggaggcct tcgacgccct
     4681 cgtgaccgac ctcaggatgc cgaagctcga cggcctgggc ctcctctcct ggcttcggga
     4741 ggagggcccc tccgtccccg tgatcgtcat ctccgcccac ggcgaggtcc gcgacgccgt
     4801 cgccgcgatg cgcctcgggg cctacgacta cctcgtgaag cccttcgatc ccgaggagct
     4861 cgtcatccgc ctcaacaagg ccgtcgcgga gcgcagggcc tcgggccggc tctccgccgg
     4921 cgagcggcgc tacgaggagg gcacccgcct cataggcgag agcaagccca tgcgcgaggt
     4981 cctggccctg gtcgagcggg cggcgccctc caacgcgaac atcctcctca cgggggagag
     5041 cggcacgggc aaggaggtcg ccgcccgcct cgtccacgag ctctccggcc gcgaggggcc
     5101 cttcgtcccc gtgaacgttg gcgccgtgcc ggagagcctc ctcgagagcg agctcttcgg
     5161 cagcgagaag ggggccttca ccggcgccga cgcgcgccgc accggccttt tcgagacggc
     5221 ccagggcggc accatcttcc tcgacgagat gggcgagctg cccctccacc tccaggtgaa
     5281 gctcctccgc gccatccagg agaagaagat catgcgactg ggcggcgtga agcccatccc
     5341 cgtcgacgcc cgcctcgtcg ccgcgacgaa ccgagacctg gaggcggagg tggccgcggg
     5401 ccgcttccgc gaggacctct actaccgcct caacgtgatc aggatccgcc tgcctccctt
     5461 gcgggaacgc tcgggagaca tcggcctcct cgcgggctac ctcctcggga agctccaggc
     5521 cgagctcggc cgccgcgtgg agggcatgag cgcggaggcc atcgccctcc tcgagtccta
     5581 ctcctttccc gggaacgtcc gcgaactcga gaacgccctg gagcgggccc tcatcctggc
     5641 cgacgggccc aggatcgagg cgcgggacct ccgcctggga agcggcggcg aggcgctcgc
     5701 gcggggacag ggctcggagg cgaaggccgg cggcccctgc gagcccttcg ccgagcccag
     5761 gagcctcgcg gagatagaga aggccgcgat cgaggcttcc ctcaggcgga accgcttccg
     5821 ccgcgagcgt acggccgagg agctagggat atcccgaagg acgctgctca acaaggtgaa
     5881 ggaatacggc atagccgtgc ccgggaggga cgacgaggag gactagcacg atgggagagc
     5941 gaaaggcgac gcggagcttc cgcggcagaa ggacgcagga cggggcgggg gtgagcctca
     6001 cgaggatctt cggcttcggg aacgaggagg agctcgatcc cttcctcctc cttgacgcct
     6061 tcggctcgaa ggatccgcgc gagtaccttc cgggctttcc ctggcatccg caccgcggca
     6121 tcgagacggt gacctacctg cttgaaggct cggtgcgcca cggggacagt ctgggcaacg
     6181 cgggcgtcat cggaccgggg gagctccaat ggatgacggc gggaagcggc atcatccacg
     6241 aggaaatgcc cctggcctcg cccgggggag tcctgggctt ccagctctgg gtcaacctgc
     6301 cccgggccga gaagatgagg gagccggcct accgcggcgt cctggccggg gaggtcccca
     6361 ggatccgcgt gccgggcggc gaagtcggcg tcctcgccgg ggattttggc ggcttccgcg
     6421 gcccagtcga gggtgtcgcg aggaatcccg cctacttcga gctcaggctg gagcccggcg
     6481 cttcgctctc cctcgaggca ccgtccgagg agacggcctt tatctacgtc ttcgagggct
     6541 cgctcgccga ggctggggcc ggggacagag cttcggctgg ggacagagct tgcgctgtgg
     6601 cgggcgattg ccttctgttc gcggcccccg gcgaggacga caagatcagc gtcgcctccg
     6661 gagccgaggg agcaaggctc atcttcgccc gggctcggcc cttgcgcgag cccatagcct
     6721 ggcgcggacc catcgtgatg aacagcgagg aggagctcga cctcgctttt tcggagttcc
     6781 acgccggcac cttcgtcaag gcgaaacgga gtcgatgaag gccctggcgt cgccctcgcc
     6841 gtagaagacg agatggacct gttctggcag ggcgtggctc gcgaggaagg ccgagacgac
     6901 gccgagcgcc acggccgcgg cctctgcctt ggggtagccg tagacgccgg tcgagatggc
     6961 ggggaaggcg atttccttga agccttcacg ggaagcaagc tcgagggagg aacggtaggc
     7021 cgaggccagg acttccgcct cgcctcggcc gccgccttca tagacgggtc cgacggtatg
     7081 gatgaccgcc ctggccctga ggcggtagcc gccggtgagc acggccttcc ccgcgggtag
     7141 gccccccggc agcctctcgg cccgaatttt cctgcaggcc tcgaggagtt ccggtccggc
     7201 cgcgcgatgg atggccccgt cgacgccgcc cccgccgagc aggcccgagt tcgcggcgtt
     7261 gacgatcgcg tctccctcga agtcggtgat gtcgcccgtt tcgacgagaa tccgcccccc
     7321 agagagttcg gccagacgct tgagcatacg aaacttcctc caaagcggag acggcgggcg
     7381 aagcgccctt tcagctctgt ccctcgaatc ctggagcggg gcgtccgtcc gatcggctcc
     7441 gtccctcgag tcttgtctct cgaggcttga gtcactacct aagaagtata caactatcta
     7501 ttggcaggac aaggacaagc gcttcgcctc tggagggccg aatctcaggc cccagctcga
     7561 gctcgatcgg ctcgccgccc gcgggtggcc gcaggctcac gcgtcgggtc gttcccagga
     7621 agtagctctc gccggcgacg ccggagagct cattgtagac tccatcggca tcggcggcga
     7681 tggaagccgc cgaagagccg ggcagggctc tcgcctcgcc ggcgccgaaa tgaaggtagc
     7741 actgtccccc tgtcgggctt ccattcgccg gccgtccttc tgccaggctc cccttcggca
     7801 gctctgtccc cgacgcgccc gacgtgcacg actcggtctc cgaagccgcc ggatccgtcg
     7861 gcaagcccct cgatgcccga agcttgccga aaggcgtcag gacgagcggt cgggggcctg
     7921 attcgatgat ccgcgtcacg ggaatgaggg ggccccttcc caggaacctc gccgcgaagg
     7981 ccgagcccgg cctcgcgtag atcgcctcgg gatcgcccgc ctcgacgact cggcctccgt
     8041 ccatgatgaa gagcctgtcg gcgatggcca gggcctcctc gaggtcgtgg gtcacatgaa
     8101 gggccgaaag ccccgcgtcc ctgatccttt cccggatctc gccgcggagg gcgcgcctca
     8161 gtgcagcgtc gagggaggag aggggctcgt cgaggaggag cagctcaggc cgcgctgcga
     8221 cgctccgcgc cagggcgacc cgctgttgct cgcccccgga aagactcgcc ggcctcctct
     8281 cgaggagccc ctcgatcctg aaggctttcc cgagttcctg cgagagcttg cggcgctcct
     8341 cggaagccgc gcctcgcaag cgggggccgt actcgatgtt gcgcctgacg gagagttggg
     8401 ggaaaagggc gtagtcctgg aagaccatgc cgacccgtcg cctctcgggg gccagaccga
     8461 ggagttcgcg gcccgcaagg cgcaccgagc cgccctcggg ctcgaggagg ccggcgacca
     8521 gcctgagggc cgtcgacttg ccgcagcccg aggcgcccag gaggactccg taggctccct
     8581 cggggagctc caggtccagg tccagggaga atccgggacg ggagaagcgg aggcctcgta
     8641 tttcaagcat ggagcgcgtc cttgtccttc gcgaagaaga cgaggccggt gacgagggcg
     8701 aggactatgc ccgcggcgca ggcctcgggg aagcggtagg aaccgacgag gcggtagacg
     8761 aggaggggaa ggggctcgaa gccgggagct ccgatgagga gcggagcgct cgcgtccccc
     8821 gcggcgatgg agaaggcgaa ggccgaggcg gtcaggaagg ctggcaggat cgatctcgcc
     8881 tcgatgtcga gggccgccct gaggcgtccc gcgccgaggg tcctcgcggc gaggaggggc
     8941 gtcctgtcga cggcggcgag cgcggccatg agactcctca agacgaaggg cagggcagcc
     9001 atcgcctcga ccaggacgac aaggaggcgg ttggcgcggg ggaagaggaa ggaccagcct
     9061 agggcgagga cgatgcccga gaccgcgaga gggagggaca gggctgtctc catggctcgg
     9121 ctccgctttc cccgcaggag gagggcggcc acgccgccca ggaccgtggc caaggccgcc
     9181 gcggggaggg ccgtcgcgag gctgtcgccc aaggctccca ggagggggcc ttggggagcc
     9241 atccagttaa cgccgcccgt ctccgcgcct ctttgaggcc cgccgaagag gagccgcgcg
     9301 aagttgccga atcccagcct cccgcctccg cccatgcccc gcctctcggt gaaggcctcc
     9361 gcgagaaggg ccgcgagggg cccggcgaag aagacgagga gaaagacggc atagacgacg
     9421 aggaagacaa aggcgggacc gacggccttg ctccttggct gcctggtgcc ggtcttcttt
     9481 gccgcctcgc cgccgcggcg ctcggcggcc gtgagcagga ggacgacgag gagggccgcg
     9541 gcggtctcga tgagggacag ggccgccgcc ccggccggat cggcgctcat ccgcgcggct
     9601 cggaagatct ccacctcgag ggtcgagccg cccaaggggc cgaagacgag gacgacgacg
     9661 aaggagaaga agcagaagag gaaaaccagg gccgaggcct ggaggatcac ggggaggagg
     9721 ctcggcagga tgccggtcgc gaaggctctg aggcgtcccg ctcccagggt cctcgccgcc
     9781 tcctcccggt cgcgcgggac attctcccag gcgtcgccca cagtctggag gacgatcggg
     9841 aagttgtaga agccgtgcac gagtacgagg ccccagaacg agtacaggaa tcccagtggc
     9901 ggctcggcga gtccgaaggc cgccatgagg aagcggttca ggtagccgtt cctgccgaaa
     9961 tagaggacga acgcgaggac gacgaggatg ggcggcacgc agaaggggag tcccgcgagc
    10021 gccttgagga ggcgccttcc gggaaactcg tgccgcgcga ccaggtaggc tcctggaaga
    10081 ccgatggcca aggcgagaag ggcgctggcg cccgcttcct tgaggccgaa gtcgagggcg
    10141 cgcaaggtgg agggcgagga aagggccgct ccgagcgact tgagcagtcc caagaagcct
    10201 ttctcgccgg ctgtggccct gaaaagggcg gccgcgaccg gcgcggccgc caagaccagc
    10261 atgaggccga gggcgaggct tcccgaaagg ccgagcttct gggttgccgg tgagactccg
    10321 gcttccgcga ctctggcttt ccttacagcc ctgtccc
//
LOCUS       DAOOSJ010000071        10322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4210_length_10322_cov_6.021428, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000071 DAOOSJ010000000
VERSION     DAOOSJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_10322_cov_6.021428"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLG14_05055"
     CDS             complement(<1..433)
                     /locus_tag="PLG14_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOX48536.1"
                     /translation="MKAVWYVALPLTGLFLGWSIRWLYARFQLAAAEQKAERIKQDAV
                     KEAEAQKKEILVEAKDQLIRERNQQEREIRERRAELQRFERRTMQKEEILDKKIESLD
                     KQEELLRGREKSALEKEEYLAGQEERYRQELERISGLSSEEA"
     gene            928..1221
                     /locus_tag="PLG14_05060"
     CDS             928..1221
                     /locus_tag="PLG14_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOX48537.1"
                     /translation="MSKKVYVGNMNYATTENGLRELFAQYGEVASVNIIVDRYTGKAK
                     GFGFVEMNTEEEARAAIEALNGFEYMGRQLRVNEAEDKPRRDGGFRSNGPRRY"
     gene            complement(1489..2634)
                     /locus_tag="PLG14_05065"
     CDS             complement(1489..2634)
                     /locus_tag="PLG14_05065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX48538.1"
                     /translation="MKKDKTRKATAREAKASRTAEAAQYRRPSDPGRPRSWEGLVGPE
                     VLDEPRVDLYLAERVGVLGRSQQKARGATIRVNGKEAKPSRPLKPGDRVELSWVEEPP
                     EGIVPERIDLKVLYEDSRSIVIDKAQGMVTHPGHGNRRGTLANALQGRLEEGGAMASQ
                     AAASQAAGAGAPRPEAAGTEAAGPEGGSAGAADFRAADFRAGVVHRLDKDTSGIIIAA
                     KDAEAQAFLASQFKERQARKEYLAVVQGRLQPSAGRVENRLGRDPRDRKRFAAVAEGG
                     KAALTEYKVLARYGQYSLVSLRPRTGRTHQLRVHLAGLGTPILGDPIYGRRDPLFPAA
                     TLMLHAYRLKIRLPGAAEASVFRAPLPGRFTALLAELERRFGKAPPD"
     gene            2766..3212
                     /locus_tag="PLG14_05070"
     CDS             2766..3212
                     /locus_tag="PLG14_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48539.1"
                     /translation="MDKEQALAKVGLFETLNPKFLKGIAGICTERSFKPGEYLMKQGE
                     SGIGLFIILSGRVRIEKTDASGKEVEIAENGPGDIMGEMAVFDGSPRSASVKAVADTS
                     CLVLASWEFNSFLKAHPEAALELLPIVVKRFRETNDALVGLRSMKN"
     gene            complement(3280..4113)
                     /locus_tag="PLG14_05075"
     CDS             complement(3280..4113)
                     /locus_tag="PLG14_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOX48540.1"
                     /translation="MRAGDSGAAAGRGESAEAGQGAAGGPAAVSAAGGAGGGGAAGPA
                     ACIFDLDGTLVDSEPNYEASDRAFLAGWGIVYDEELNRAVMGRGIKEALAIFESRFPA
                     SPYHALPVAERIRLKDEAYLAYARGRTRAFPSMLALARGLSLRGFPLAVASGSSPEVI
                     ALCLSEAGYDGLFGAVVSAAEVPRGKPAPDVFLEAARRLGTPPGRCLVFEDTLVGLEA
                     ARAAGMACVLLPMPGQGERPGEEAALRVFRGGPAAASLSELLALAEGFAASLAADSVR
                     A"
     gene            complement(4110..4994)
                     /gene="truA"
                     /locus_tag="PLG14_05080"
     CDS             complement(4110..4994)
                     /gene="truA"
                     /locus_tag="PLG14_05080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOX48541.1"
                     /translation="MTEGTRNIRLTLSYDGGEFSGWQRQKADRSVQEELERALGRMHG
                     HEVPTVGAGRTDSGVHALGQVANFYTDIRSIPAGKFVPALNKLLPRDLRILESREADF
                     DFHSRYDARLRRYRYFVLCGGAQDPFRLRYAHQLHRRPDAAVLNAMAGTLLGETDFTA
                     LSSAKDASLSRSRFVHEASFRWEGDLLVFEIAANAFLWRMVRSVVGSLLSFEAEGKGP
                     AEFRALLDSRDRSLAGPTAPARGLFLWNVEYYASPTRPGRGDYWATRGPAARDDEAGG
                     SGDGVLEGGPAAAGMGEE"
     gene            complement(4987..5874)
                     /locus_tag="PLG14_05085"
     CDS             complement(4987..5874)
                     /locus_tag="PLG14_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOX48542.1"
                     /translation="MGRIAAKPEKEDKEKKISFIAKESIRCPVCDAVFRREELFSGRV
                     NASDLTDELHRTYTPMQAYGEVTPLVYELTVCPSCWYSALKYDFADPPARTRQALQDG
                     ISVRVEAVQRLFEPLDFGEPRGLLEGAASYYLALLSYELFPKDYSPVIKQAVAALRAA
                     WLCKDLERRRPGENFGYVAEIFYRKARFLYRLAVELEQKGVQRLAEAKWLGPDTDKNY
                     GYEGVLYMQGILDLKYGPRGDEAKRAESLEASKRAIAKMFGLGKRSKSKPGPLLDKAR
                     DLYDSLKAELKQDDDDGDD"
     gene            complement(5877..6248)
                     /locus_tag="PLG14_05090"
     CDS             complement(5877..6248)
                     /locus_tag="PLG14_05090"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOX48543.1"
                     /translation="MILGIGVDIVHVKRIHHWLSVPGLVDRFFHPDEIVAAQERGHSA
                     ALSLAARFAAKEAFGKALGTGLAGIVLRDIQVINNHNGRPDMHLFGTALSRFQDLGGK
                     RALLTMTHESDNAVAVVVIEG"
     gene            complement(6245..7201)
                     /locus_tag="PLG14_05095"
     CDS             complement(6245..7201)
                     /locus_tag="PLG14_05095"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOX48544.1"
                     /translation="MRNKGFWARLFSDWPAKVLSFAAALLLFFFYRLNRLEERYLSVP
                     VSVQMNEEYVPGSQYPRSVRVTLRGESNALYAIQEEDLRAVLDLSSERGEGVVKAPVV
                     IEKRGNALGVDPLEILAEPAEIAVAMERKVSRIVPVTPAFRGYLEPGYELVSFDFSPA
                     EVEIAGPASAVARVVDIATESIELSGRREDFTEKARLARRDPLILVVGSDSVEFRATV
                     QRSLAVKTFDSLEIAVRGLAEGLDLEEPLPFGSLRIQSSKADLRGYEPEPGALYVDFS
                     AVRRPGAYTLPVAADLPPDFALDSYLPATVAVRVVAKKEAAP"
     gene            complement(7191..7997)
                     /gene="cdaA"
                     /locus_tag="PLG14_05100"
     CDS             complement(7191..7997)
                     /gene="cdaA"
                     /locus_tag="PLG14_05100"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOX48545.1"
                     /translation="MGQILSGGFLADYLRPALDILLLAFLVYKTWELLVRTQAIQLVK
                     GALILAGFYAAAFFLQLTTVTWVLNILAPGLVIALAIVFQPELRKIFMRLGQGSLLRG
                     GSRPRVGQIEAILNAAETLSAGRRGALVVFARNVGLKNIVDTGTRLEALISSSLIVSI
                     FGHDTPLHDGAVIVMDGRIEAAGCFLPLSEQQDIRKSFGTRHRAALGLSEEADAVVLV
                     VSEETGALSLAYDSKLYYGLSPDQVRRRLGELLEFPADQADSAEEAPHEE"
     gene            complement(8010..8876)
                     /gene="folP"
                     /locus_tag="PLG14_05105"
     CDS             complement(8010..8876)
                     /gene="folP"
                     /locus_tag="PLG14_05105"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOX48546.1"
                     /translation="MTRLPLPRGRFLELEPFAVMAIVNATPDSFYEPSRARAALEARD
                     AALAALAAGARVVDIGGESTRPGSDYVGEEEELERVVPVVEALRRESDAPISVDTRKA
                     AVARAALAAGADIVNDVSALLHEPETAKIAAAAGAPVVLMHMKGEPKTMQAAPSYGDC
                     PREVAAFLLEAAARAEAAGIPRDRIILDPGIGFGKRLEDNLSLLSRLDELADLGYPLL
                     VGLSRKSFVGAIAGRPPEGRLAGSLGAACAAFAKGARIFRVHDAAATLDALALFSASL
                     RGGAGAAQAAGR"
     gene            complement(8873..>10322)
                     /locus_tag="PLG14_05110"
     CDS             complement(8873..>10322)
                     /locus_tag="PLG14_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOX48547.1"
                     /translation="FEALSHAGQLGLPLVVVLNDNKMSISPNVGALSRYLSRLSATVR
                     YQNFRARVDSLVRRVPLVGGLLYGLIVRAKRAVKAMFFKENLFADLGFEYAGPIDGHN
                     LPAMLQVLREVRSLERPVVVHVLTRKGKGYELAEDDPARYHGVSPMRVQDGKVEARPA
                     VTFTEAFSESLIARGFGDPSVVAVTAAMAKGTGLEEFRAACPGRVYDVGIAEQHAVTF
                     AAGLAAGGMRPVVAIYSTFMQRAVDQVFHDVALPGLPVVFAVDRAGAVEGDGETHQGL
                     WDLALFRSMPNMAILAPASARELDLCLGYALAAPGPVMIRYPKARCPAEEAAYAEPLV
                     AGRGVFARRSGADTLLVALGGLVPLAVEASDLLLREGRATDVYSLRFAAPLDEEAFLE
                     AAAPYRRIILFEEGVAAGGVGESLAALAARRLPRLSFAALGFPSEPYAQGARAELLEK
                     AGLSAQAMAERVRLDEEDAGRIYLFRAKDKDE"
BASE COUNT         1525 a         3465 c         3802 g         1530 t
ORIGIN      
        1 tggcttcctc ggaggagagg ccggagatgc gctccagctc ctgccggtag cgctcctcct
       61 ggcccgcgag gtactcctcc ttctcgaggg cggacttctc ccggccccgg aggagctcct
      121 cctgcttgtc cagggactct atcttcttgt cgagtatctc ttccttctgc atcgtccgcc
      181 gctcgaagcg ctggagctcc gcgcggcgtt ccctgatttc ccgttcctgc tggttccgtt
      241 cccgaatgag ctgatctttc gcctctacca ggatctcttt cttctgcgcc tcggcctctt
      301 tgaccgcgtc ctgcttaatc cgctccgcct tctgctcggc ggcggcgagt tgaaatctgg
      361 cgtaaagcca gcggatcgac cacccaagga atagaccagt aagaggaagg gctacgtacc
      421 agacggcctt catggttccc cctactatag atttttcgcg tccgcttcga ttatacggat
      481 gcgagtccat ttgtcaaggt ttccggcgcc cccgccgatc gtgaatttcg ttgcttttac
      541 agaaattacg attgacggcg catatgtcgg cgtctatagt catggtggaa tcccgaatgg
      601 gattccgcgt actttcggag ggatgagttc gccttctcta tgtttgaggt agaaaaccgg
      661 gtccgcaaca ttgtccgtac ctacacggag cggcgctctc gcccggcggc cgggcggggg
      721 aatctccgcg gtccacacgt cgcaggcaag gaaagcacaa aactttctcc accctcgccc
      781 gttttcggct ccttaaagtc agttgggatc tctgaacggc tctccagaaa gccgccgtac
      841 gcccgcgaaa gcgggcgcat tcgccatggg cggatgaatc cggcgcggac gggcgcgagc
      901 ccggccagcc acgtatgagg ttttcgcatg tccaagaagg tttacgtcgg caacatgaac
      961 tacgctacca ctgagaacgg gctcagggag ctcttcgccc agtacggcga ggtcgcctcg
     1021 gtcaacatca tcgtcgatcg ctacaccggc aaggccaagg gcttcggttt cgtcgagatg
     1081 aacaccgagg aggaggcgcg cgccgctatc gaggccctca acggcttcga gtacatgggc
     1141 cgccagctcc gcgtcaacga ggccgaggac aagccccgcc gcgacggtgg tttccgttcc
     1201 aatggtcccc gccgctacta actgacttta agaagccggc cggaagcctg aggcttccgg
     1261 ccgcccggta ctggaaagga gcgggccccc caagggcctg cgagccgcgc ggtggccgcc
     1321 agcgcggctt ttttacgcca gaaggcctgt gccgcgaccc acagccggcc tccgcggccc
     1381 ccggcgccat cagacatggc tccccgatac ggccgccgcg gccggcggcc ggtcccccgg
     1441 cgaaggccgg gcgcggcctt cagtccggtg gctcccggcc cggcgccctc agtccggcgg
     1501 ggccttgccg aagcgccgct cgagctcggc gaggagggcc gtgaagcggc ccggcaaggg
     1561 cgccctgaag acggaggcct cggccgcgcc gggaagcctg atcttgagcc tgtaggcgtg
     1621 gagcatgagg gtcgcggccg ggaaaagggg gtcgcgcctg ccgtagatcg ggtcgccgag
     1681 gatgggcgtc cccaggccgg ccaggtggac gcggagctgg tgggtccgcc ccgtcctggg
     1741 cctgagcgag accaggctgt actggccgta gcgcgccagg accttgtact cggtcagggc
     1801 cgccttcccg ccctccgcga cggcggcgaa gcgcttgcgg tccctgggat cccggcccag
     1861 gcggttctcc acgcggccgg ccgagggctg gaggcgcccc tggaccacgg ccaggtattc
     1921 cttcctcgcc tggcgctcct tgaactgcga ggcgaggaag gcctgggcct ccgcgtcctt
     1981 cgcggcgatg atgatgccgg aggtgtcctt gtcgaggcga tgcacgacgc cggcgcgaaa
     2041 atccgcggcg cggaagtccg ctgcgccggc gctcccgcct tcggggcccg cggcctcggt
     2101 acccgcggcc tcgggacgag gggcgccggc gcccgccgcc tgggaagccg ccgcctggga
     2161 ggccatcgct ccgccctcct cgagcctgcc ctggagggcg ttggccaagg tgccgcggcg
     2221 gttgccgtgg ccgggatggg tcaccatgcc ctgggccttg tcgatgacga tggagcggga
     2281 gtcctcgtag aggaccttga ggtcgatgcg ctcggggacg atgccctcgg gcggctcctc
     2341 gacccaggag agctcgaccc tgtcgcccgg cttgagcgga cgggagggct tggcctcctt
     2401 cccgttcacg cggatcgtgg cccccctcgc cttctgctgc gagcgcccca ggaccccgac
     2461 cctctccgcg agatagaggt ccacccgggg ctcgtccagg acctcgggcc cgaccaggcc
     2521 ctcccagctc cggggcctgc ccgggtcgga gggccggcgg tactgggccg cctccgccgt
     2581 ccgggaagcc ttggcctccc gggccgtggc ctttctcgtc ttgtccttct tcatttggct
     2641 cgtcgacctt tcatgcgccg attggcgagg gcggctcgag cgccgcgaag ccccggaaac
     2701 cacatcgtcc ttgacggctc ccgcctcctc ctctatctta ctcatacgta gaaaggagcg
     2761 cgagcatgga taaagaacag gcccttgcga aagtgggcct ttttgagacc ctcaacccga
     2821 aattcctcaa aggcatcgcc ggcatctgca ccgagcgcag cttcaagccc ggcgagtacc
     2881 tcatgaagca gggcgagagc ggcatcggcc tcttcatcat cctctccggc cgcgtccgca
     2941 tcgagaagac cgacgcctcc ggcaaggaag tcgagatcgc ggagaacggc cccggcgaca
     3001 tcatgggcga gatggccgtc ttcgacggct ccccccgctc cgccagcgtc aaggccgtgg
     3061 ccgacacgag ctgcctcgtc ctggcctcct gggagttcaa ctccttcctc aaggcccatc
     3121 ccgaggccgc cctcgagctc ctccccatcg tcgtgaagcg cttccgcgag acgaacgacg
     3181 ccctcgtggg cctcaggtcg atgaagaact aaaggtcccc gcgggcgagc cgcggacctg
     3241 cgagcggggc cgtccttcgg ggcggccccg ttttttcgcc taggcccgga ccgagtcggc
     3301 cgcgagcgag gcggcgaagc cctcggcgag ggcgaggagc tcggaaagcg aggcggccgc
     3361 ggggccgccg cggaagaccc gcaaggcggc ttcctcgccg ggccgctcgc cctgccccgg
     3421 catggggagg aggacgcagg ccatgcccgc cgcccgggcc gcctccaggc cgaccagggt
     3481 gtcctcgaag acgaggcagc gccccggcgg cgtcccgagg cggcgggccg cctccaggaa
     3541 gacgtcggga gcgggcttgc cccggggcac ctcggccgcg gagaccaccg cgccgaagag
     3601 gccgtcatag cccgcctccg agaggcagag ggcgatgacc tcggggctgg agcccgaggc
     3661 gacggccagg gggaagccgc gaagggaaag cccgcgggcc agggcgagca tcgaggggaa
     3721 ggccctggtc ctgcccctgg cgtaggccag gtaggcctcg tccttgagcc tgatcctttc
     3781 ggcgacgggc agggcgtggt aggggctcgc gggaaagcgg ctctcgaaga tggccagggc
     3841 ctccttgatg ccgcggccca tcacggcgcg gttgagctcc tcgtcgtaga ctatgcccca
     3901 gccggccagg aaggcgcggt cgctcgcctc gtagttaggc tcggagtcga cgagggtccc
     3961 gtccaggtcg aagatgcagg ccgcgggccc ggcggcgcct ccgccccctg ccccgccggc
     4021 cgccgagacg gccgccgggc ctcccgcggc gccctggccc gcctctgcgg attctccgcg
     4081 gccggccgcc gcgcctgagt ctcccgctct cattcctccc ccatccccgc cgcggcgggg
     4141 ccgccctcaa ggacgccgtc cccgcttccg ccggcctcgt cgtcgcgcgc ggccggcccc
     4201 cgcgtcgccc agtagtcgcc gcggcccggc ctcgtgggcg aggcgtagta ctcgacgttc
     4261 cagaggaaga ggccccgggc cggcgcggtc ggccccgcga ggctccgatc ccgcgaatcg
     4321 aggagggcgc ggaactccgc ggggcccttg ccctcggcct cgaaggagag gaggctcccc
     4381 acgacggagc ggaccatgcg ccaaaggaag gcgttggccg ctatctcgaa gaccaggagg
     4441 tcgccctccc agcggaagga ggcctcgtgg acgaagcggg agcgggagag gctcgcgtcc
     4501 ttggcggagg agagggcggt gaagtccgtc tccccgagga gggttccggc catcgcgttg
     4561 aggacggccg cgtccggccg cctgtggagc tggtgcgcgt agcggagcct gaaggggtcc
     4621 tgggccccgc cgcagaggac gaagtagcgg tagcgcctga gcctcgcgtc gtagcgcgaa
     4681 tggaagtcga agtccgcctc gcgggactcg aggatccgca ggtcgcgggg gaggagcttg
     4741 ttgagggccg gcacgaactt gccggcgggt atggagcgga tgtcggtgta gaagttggcg
     4801 acctggccca gggcgtggac gcccgagtcc gtgcggccgg cgccgaccgt gggcacctcg
     4861 tggccgtgca tcctcccgag ggccctctcg agctcctcct ggacggagcg gtccgccttc
     4921 tggcgctgcc agcccgagaa ctcacccccg tcgtaggaga gggtgaggcg gatgttcctt
     4981 gttccctcag tcatcgccgt cgtcgtcgtc ctgcttgagc tcggccttga ggctgtcgta
     5041 gaggtcccgc gccttgtcca ggagggggcc gggcttggac ttgctccgct tccccagccc
     5101 gaacatcttc gcgatggcgc gcttggaggc ctccagggac tcggcgcgct tcgcctcgtc
     5161 gccccggggc ccgtacttga ggtccaggat gccctgcatg tagaggacgc cctcgtagcc
     5221 gtagttcttg tcggtgtccg ggccgagcca cttggcctcg gccaggcgct ggacgccctt
     5281 ctgctcgagc tccacggcca agcggtagag gaagcgcgcc ttgcggtaga agatctccgc
     5341 gacgtagccg aagttctcgc ccgggcggcg ccgctccagg tccttgcaga gccaggccgc
     5401 ccggagggcc gcgacggcct gcttgatgac gggcgagtag tccttgggga agagctcgta
     5461 ggagaggagg gccaggtagt agctcgcggc gccctccagg aggccgcgcg gctcgccgaa
     5521 gtccaggggc tcgaagaggc gctggacggc ctcgacccgg acggagatgc cgtcctggag
     5581 ggcctgccgc gtcctcgcgg gcgggtcggc gaagtcgtac ttgagcgcgg agtaccagca
     5641 ggaggggcag acggtgagct cgtagacgag gggggtcacc tcgccgtagg cctgcatcgg
     5701 cgtgtaggtc cggtggagct cgtcggtgag gtcgctcgcg ttgacgcggc cggagaagag
     5761 ctcctcgcgg cggaagaccg cgtcgcagac cgggcagcgg atcgactcct tcgcgatgaa
     5821 ggagatcttc ttctccttgt cctctttctc gggcttggcc gcgatccttc ccatggctag
     5881 ccctcgatga cgacgacggc cacggcgttg tcgctctcgt gcgtcatggt gaggagggcc
     5941 cgcttcccgc ccaggtcctg gaagcgggaa agggccgtgc cgaagaggtg catgtcgggc
     6001 ctgccgttgt ggttgttgat cacctggatg tccctgagca cgatgcccgc gaggcccgtc
     6061 ccaagggcct tgccgaaggc ctccttggcc gcgaagcggg cggccaggga gagggcggcg
     6121 gaatggccgc gctcctgggc cgcgacgatc tcgtcggggt ggaagaagcg gtccacgagg
     6181 ccggggacgg agagccagtg gtggatgcgc ttcacgtgga cgatgtcgac gccgatgccg
     6241 aggatcacgg ggcggcctcc ttcttcgcga ctacgcggac ggcgacggtc gccggcaggt
     6301 aggagtccag ggcgaaatcc ggcggcaggt ccgccgccac ggggagggtg taggcgccgg
     6361 gccggcgcac ggcggagaag tccacgtaga gggcgcccgg ctcaggctcg tatcctcgca
     6421 ggtcggcctt ggaggactgg atccttaggc tcccgaaggg caggggctcc tccaggtcga
     6481 gcccctccgc gaggccgcgg acggctatct ccagggagtc gaaggtcttc acggccaggg
     6541 agcgctggac cgtcgcccgg aactctacgg agtcggagcc gaccacgagg atgagggggt
     6601 cgcggcgcgc cagcctggcc ttctcggtga agtcctcgcg gcgtcccgag agctcgatgg
     6661 actcggtggc gatgtcgacg acccgggcca cggcgctcgc agggccggct atctccacct
     6721 cggcggggct gaagtcgaag gagacgagct cgtagccggg ctccaggtag ccgcggaagg
     6781 ccggcgtcac gggcacgatc ctggagacct tcctctccat ggcgacggct atctcagcgg
     6841 gctcggcgag gatctccagg ggatcgacgc ccagggcgtt gccgcgcttt tctatgacga
     6901 cgggggcctt cacgacgccc tccccccgct cggaggagag gtccaggacg gcccgcaggt
     6961 cctcctcctg gatcgcgtag agggcgttcg actcgccccg cagggtgacg cggacgctcc
     7021 gcgggtactg gctcccgggg acgtactcct cgttcatctg gacggagacg ggcacggaga
     7081 ggtagcgctc ctccagccgg ttcaggcggt agaagaagaa gaggaggagg gcggcggcga
     7141 aggagaggac cttcgccggc cagtccgaga agagcctggc ccagaagccc ttattcctca
     7201 tggggggcct cctcggcgct gtcggcctgg tcggccggga actcgaggag ctccccgagg
     7261 cggcgcctga cctggtcggg ggagaggccg tagtagagct tcgagtcgta ggcgagggag
     7321 agggcgcccg tctcctcgga gacgaccagg acgacggcgt ccgcctcctc cgagaggccc
     7381 agggcggccc ggtgcctggt cccgaagctc ttccggatgt cctgctgctc ggagaggggg
     7441 aggaagcagc cggcggcctc gatcctcccg tccatcacga tgacggcccc gtcgtggagg
     7501 ggcgtgtcgt ggccgaagat agagacgatg aggctcgagg agatgagggc ctccagcctc
     7561 gtgcccgtgt cgacgatgtt cttgaggccg acgttccgcg cgaagacgac gagggcgccg
     7621 cggcggcccg cggagagggt ctccgccgcg ttgaggatgg cctcgatctg gccgacccgg
     7681 ggcctcgagc cgccgcggag gagggaaccc tgccccaggc gcatgaagat cttccggagc
     7741 tcgggctgga agacgatcgc gagggcgatg acgaggccgg gcgcgaggat gttgaggacc
     7801 caggtcaccg tcgtgagctg gaggaagaag gccgcggcat agaagcccgc gaggatgagg
     7861 gcgcccttca cgagctggat cgcctgggtg cggaccagga gctcccaggt cttgtagacg
     7921 aggaaggcga ggaggagtat gtcgagcgcc gggcggaggt agtccgcgag gaatccgccc
     7981 gagagaatct ggcccacgcc tcccccttcc tatcggccgg cggcctgagc ggctccggcc
     8041 ccgccccgga gcgaggccga gaagagggcc agcgcgtcca gggtcgccgc agcgtcgtgg
     8101 acgcggaaga tcctcgcgcc cttcgcgaag gcggcgcagg ccgccccgag gctcccggct
     8161 agccggcctt cgggtggcct tccggctatc gcgccgacga aggacttccg cgagaggccg
     8221 accaggagag gatagcccaa gtcggcgagc tcgtcgagcc gggaaaggag ggagaggttg
     8281 tcctccagcc gcttcccgaa gccgatgcca gggtcgagga taatgcggtc ccgggggatt
     8341 ccggccgcct cggcccgggc ggcggcctcg aggaggaagg ccgcgacctc gcgagggcag
     8401 tccccgtagg agggcgcggc ctgcatggtc ttgggctcgc ccttcatgtg catgaggacg
     8461 acgggggcgc cggcggccgc ggctatcttc gcggtctcgg gctcgtggag gagggcggag
     8521 acgtcgttca cgatgtcggc gccggccgcg agggcggcgc gggccacggc cgccttccgc
     8581 gtgtccacgg agatgggcgc gtcgctctct cggcgcaggg cctccacgac ggggacgacg
     8641 cgctcgagct cctcctcctc gcccacgtag tcggagcccg gcctcgtcga ctcgccgccg
     8701 atgtccacga cgcgggcgcc ggcggccagg gcggccaggg ccgcgtcgcg ggcctccagg
     8761 gccgcgcggg cgcggctcgg ctcgtagaag gagtcgggcg tcgcgttgac gatggccatc
     8821 acggcgaaag gctcgagctc gaggaagcgg ccgcgcggga ggggaaggcg cgtcattcgt
     8881 ccttgtcctt agcgcggaag agatagatcc tgcccgcgtc ctcctcgtcc aggcggacgc
     8941 gctcggccat ggcctgggcg gagaggccgg ccttctccag gagctcggcc ctggcgccct
     9001 gggcgtaggg ctcggagggg aagcccaaag ccgcgaagga gaggcgcggc aggcgccgcg
     9061 cggcgagggc cgcgagggac tcgccgacgc cgcccgccgc gacgccctcc tcgaagagga
     9121 tgatgcggcg gtaaggcgcg gcggcctcga ggaaggcctc ctcgtccaag ggggcggcga
     9181 agcgcaggga gtagacgtcc gtggcccggc cctcgcggag gaggaggtcg gaggcctcga
     9241 cggccagggg cacgaggccg cccagggcga cgaggagggt gtccgcgccc gagcgtctcg
     9301 cgaagacgcc gcggcccgcg accaggggct cggcgtaggc ggcctcctcc gcggggcagc
     9361 gggccttggg gtagcggatc atcacgggcc ccggcgcggc cagggcgtag cccaggcaga
     9421 ggtcgagctc gcgggcgctc gcgggcgcga ggatggccat gttgggcatg ctgcggaaga
     9481 gggccaggtc ccagaggccc tggtgggtct cgccgtcgcc ctccacggcg ccggcgcggt
     9541 ccacggcgaa gacgacgggg aggcccggga gcgcgacgtc gtggaagacc tggtcgacgg
     9601 cgcgctgcat gaaggtcgag tagatcgcga cgacgggcct catgcccccc gccgccaggc
     9661 ccgccgcgaa ggtgaccgcg tgctgctccg cgatgcccac gtcgtagacg cggcccgggc
     9721 aggctgcgcg gaactcctcc aggcccgtgc ccttcgccat cgccgccgtc acggccacca
     9781 cggaggggtc gccgaagccg cgcgcgatga gggactcgga gaaggcctcc gtgaaggtga
     9841 cggcgggccg ggcctccacc tttccgtcct ggacgcgcat cggcgagacg ccgtggtagc
     9901 gggcggggtc gtcctccgcg agctcgtagc ccttgccctt ccgcgtgagg acgtggacga
     9961 cgacggggcg ctccagggag cggacctcgc ggagcacctg gagcatcgcg ggcaggttgt
    10021 ggccgtcgat cgggcccgcg tactcgaagc ccaggtcggc gaagaggttc tccttgaaga
    10081 acatcgcctt caccgcccgc ttggcgcgga cgatgaggcc gtagaggagg ccgcccacga
    10141 gggggacgcg gcgcactagg gagtcgacgc gggcccggaa gttctggtag cggaccgtcg
    10201 ccgagagcct ggagaggtag cgggagagcg cgccgacgtt gggcgaaatc gacatcttgt
    10261 tgtcgttgag gacgacgacg agggggagcc cgagctgtcc cgcgtgggac agggcctcga
    10321 ag
//
LOCUS       DAOOSJ010000072        10284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4236_length_10284_cov_5.704272, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000072 DAOOSJ010000000
VERSION     DAOOSJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4236_length_10284_cov_5.704272"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLG14_05115"
     CDS             complement(<1..232)
                     /locus_tag="PLG14_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48548.1"
                     /translation="MRRPLSSFAPAALIALALAATGCQQLFTTSLAAWAERDSISIPK
                     DISNADAAEILATTDNPTVLSALLAVLNDQAAA"
     gene            complement(317..1483)
                     /locus_tag="PLG14_05120"
     CDS             complement(317..1483)
                     /locus_tag="PLG14_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48549.1"
                     /translation="MKRTTFVLALVLLAASLAGAQTAALSPLSAKDPRSMALGGAFTA
                     LSSGYSALSGNPAGFAAAKAELMVADFSSWLYVKPTSANLEKAARLASGGADDAEILS
                     AMNDLITENGFGGGLSFGAGYVGKGLGVGMFLVGDSSVKGETAMGAVMTSSASLNAVL
                     GLGAPIRIGGLTVSLGGDVRPFFRAESADGGWAFGEIMSGLMSGGDVGAVFMGESVES
                     GFGLAMDFGASVELGSLSVGLSIRDVAPSFIVSEMTVEELLAELGAGSFPRASCSSQS
                     VLVLPSVTAGLAWKPRLVPGLVEPALYFEVQDPVSVIRDRESFWNLLHVGAEVELLSF
                     FSLRAGLNKGWPSAGFGLDLAFLSLDAAVFTEELGRRPGDLPRSGATISAAIRF"
     gene            complement(1497..2291)
                     /locus_tag="PLG14_05125"
     CDS             complement(1497..2291)
                     /locus_tag="PLG14_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48550.1"
                     /translation="MKPKLAVLAVGSFLILASCDAFFSTNLFKEAGLGQVKPSDLTEM
                     SSDDLFEAAYFEGGTSSTFMEALPYDSTVQTVVLATLQTTYNDSLAAPVARQEAAALA
                     VDILIETSGASAIVKEVIPAIVGGLDLPSIDTPEEIVDLVELVVPAELRADMVAFKTA
                     IAALLRADAVLTNPAGVETLIEGSGVSGDYSVGTLAQNAVVAAAVAGVSPVSGTIEDA
                     LWSAVTDSSNAASYINSTYNAPDLASGSMLNILLTEAGLDPSKFGY"
     gene            complement(2401..3891)
                     /gene="mtaB"
                     /locus_tag="PLG14_05130"
     CDS             complement(2401..3891)
                     /gene="mtaB"
                     /locus_tag="PLG14_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOX48551.1"
                     /translation="MDVRVAFRTLGCKLNQLESESLADAFRRAGARIGAFESEAELYV
                     LNTCTVTSKAEQKARRLVRQALALNPEAVVLVTGCYAQMEPAAIEALGERAVVVPGDE
                     KAALLGLASHLSEYWQGHGRLLDAVLEWRLGLEGESGGQEGGGRAGGADRFAFRPESF
                     SFHSRPSLKIQDGCDNRCTYCRVCLARGPSTSLPATEVLSRLRALEEAGAAEAVLTGV
                     NLSQYRDGSLGFPGLLRLLLSGTERIALRVSSYEPDKVDEAFLAAFAEPRVRPHAHLP
                     IQSGSDPVLARMGRAYRRDAVLAACEGLRRAKGDLFLAADLIVGFPGETDADFAQTLE
                     LAKAADLAWIHAFPFSPRPGTKAWDMSGRVPERIAGERAAALGELARKGRAAYVERWL
                     GAEVDAVLESGGERDEEEGALAEGAVVVGGAGGDAAESASLGRAQGRPARYATSANYL
                     KLRVEGLPEAPALKPGAGIRCRLKAGSGEGVLPLADAVARAEFLHS"
     gene            complement(3895..4617)
                     /locus_tag="PLG14_05135"
     CDS             complement(3895..4617)
                     /locus_tag="PLG14_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48552.1"
                     /translation="MRASSPAGDSRRTRGRLGRARGGPVGAYGRRGVAAARGAALLLA
                     LLLCSAAAASAAEGDALKLFQEGRHEDARRVSLEALRADPADVESYVVLCWSLLALER
                     WADAENYALKAYAIRKDPRVTEILGESAFRLGRNEAALSRFQDYVSAVPEGSRVGTAY
                     YYMGEIYLRMARYAHADIALSSALKFAPGNARWWARLGWAREKAGETQGALAAYAEAL
                     RLDPRQEDARLGAERLQARSRG"
     gene            complement(4607..4975)
                     /locus_tag="PLG14_05140"
     CDS             complement(4607..4975)
                     /locus_tag="PLG14_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOX48553.1"
                     /translation="MTEVQISAVDEEQLDTVLAGDVEFSGSMTFKKPLMIKGRVSGVI
                     RSESELYIDEKAVVEADIKALVVSVKGSVKGDIAARERVELFASSSVDGDVTAPQITM
                     ETGCRFNGACKMAAPDFDAR"
     gene            complement(4972..5733)
                     /gene="rsmA"
                     /locus_tag="PLG14_05145"
     CDS             complement(4972..5733)
                     /gene="rsmA"
                     /locus_tag="PLG14_05145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOX48554.1"
                     /translation="MWEIGPGIGSMTAMALDSGLEVTAFEIDRGFARLLGRIFAGRPK
                     FRLVEGDFLRTWKTELAAGRLPDAVFGNLPYNVASAMVALLIERYPEAAGSGGPPPMV
                     FTVQKEAALRMAAKPGTKDYSAFSVLCASACRVRIAFDLAGGSFWPVPNVQSSLVVLE
                     PRPDPIAPADRLGFSAFVRSAFSSRRKTLKNNLKAAGRPEAEVAAALEALGFSPSIRA
                     EALRPEELAAVYGRLKAAPVPPLPTGAPGPYDAEL"
     gene            complement(5822..7864)
                     /locus_tag="PLG14_05150"
     CDS             complement(5822..7864)
                     /locus_tag="PLG14_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOX48555.1"
                     /translation="MPFVFLEEEPRLFSPPWALGLGLAFGFYFLRLTPGAVLVAAMAA
                     AAAGSVLLGLRARAPRLRAILCLAAALGLALGAALRRDEAGAAVAPLLPASPLPPAAA
                     RPPAAPHPPLGAPIGAAAGLAVGGSAAGAASRPLSVVGVEGRLSADSTLSQSGFRSYP
                     LSLTALLLEGPGLAGWVEASGPCRLLVRGGEVLEAGAKIAARGRPGGATAAAASGLSA
                     SRPAVAGGSAARPAERSDPAASPAPQGPPAFFADPGGLQTWTGGGPVAALRSSLRRRF
                     RAALGLAGGEGAASGLLVALLMGDRGGLDLEEAVDWKRAGCAHVLALSGQHLAILAAL
                     ATFLAKPLLGPRKARLAALLLALAFVFMAGASPSLLRSLLMYSLGALAFFADRPQPGQ
                     TILALAFAVALPLDPAAARSLSFELSYLALAGLVLLAPRFEFLLLPVFPPFLAKALAA
                     GLAAQAATAPLLAFTFGTLYPAGLLASVLSALLVAAFMWWGLAAALLCGFCPALAPLA
                     APVSRLLHGLLSGTMARFAAWPELALPGRRGAALASALVVLGACFVYALPHVEHRIWL
                     RRLAAGERPGAVPRGLRPGERRPDEAPRAQVRLPGLPARPPGRGGARDVEALRPELPR
                     KSDLPRAHPRGASQGPFRQPEERRGRRRQGWRRRKSRRRRQGRRRGRGRRGYVRAH"
     gene            complement(7901..9100)
                     /locus_tag="PLG14_05155"
     CDS             complement(7901..9100)
                     /locus_tag="PLG14_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48556.1"
                     /translation="MKVRAVVAALALVLAFAPPEARAQDAGSVLLTYQRNFVRSSLGA
                     KLELLKEAGARNAEEMAPLYDTALRFAVESEPLLGAADAQLRELVLIAAAKAGEAAYA
                     PSAARLWALFRLYEGDVELRALAVASLGAAGAGNEEVVAAVGEFLASQNVVFRSGMQP
                     EYPALEAAVDALGRIGDGASFPVLFSTYVAGYNPDLTAKASKALGRIKGDYAGYLARV
                     LAMSSPLEKAAALKAALENPAFGPEERGGLAEAALAAGLAWTGGAPVENQAIRELNLR
                     AIRTIRELGWQRASPLVLRYFRALLADYAKGAAPRGDLLETIVCLGAMGSSEAAQALA
                     LQLQLINAQTELGGSYDDEVILATIRALGELGDKVAFDYLLYVGYLKYPDEVKRAAKE
                     ALLRLRW"
     gene            complement(9114..10166)
                     /locus_tag="PLG14_05160"
     CDS             complement(9114..10166)
                     /locus_tag="PLG14_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX48557.1"
                     /translation="MPMTTLFLLVIVLSIVTLVLAVRGGQRHSWLEFQLRAKDSGFGY
                     GESRILREAASLCRIADPASLFSSPRDLDRAIAVLLARQRDLGRDRSREGIALMDKVY
                     ALRSRIEFEQPRYKSGIRSSRQLSQGQRLRVLVHGLGVYGSTVIDNNGRYLVLSYPSG
                     ARLPKNFVWKGKKVSIYFWRRDDAGYVFDSYAIDDLRIRNVPVIHVAHSESLLRTQKR
                     KSVRSRTSLPAYLYLLKRIEGAFEKPERDPGLRCRVEDISEDGFSVAIGGRGKAGLQV
                     KVQFGLEGRQIVMSGVARSVDYDSEKNRSVLHVEASPPSPRTRNAIRSYVYKIRAEGE
                     LEPLSVESEEAGAGVS"
BASE COUNT         1686 a         3319 c         3950 g         1329 t
ORIGIN      
        1 cggccgcggc ctggtcgttc agcacggcga gcaaggcgga caacacagtc ggattgtcgg
       61 tcgtcgcgag tatctccgcg gcgtcggcgt tgctgatgtc cttcggtatc gagatggaat
      121 cccgctcggc ccaggccgcg agcgaggtgg tgaagagctg ctggcagccc gtcgccgcga
      181 gggcgagggc gatgagggcg gcaggggcga aggaggaaag gggacgccgc atcgagtgcc
      241 tcctggtcgt cgggagggcc gccttgggca ggccctccct ttgggattat cggcagcgcg
      301 ggcgtcccgc tacaggctag aagcggatcg ccgcgctgat agtcgctccg gagcggggca
      361 ggtcgcccgg ccgccggccg agctcctccg tgaagacggc cgcgtccagg ctgaggaagg
      421 ccaggtccag gccgaagccg gcggagggcc agcccttgtt gagcccggcg cggagcgaga
      481 agaagctcag gagctctacc tcggcgccga catggaggag gttccagaag gactcgcggt
      541 cgcggatgac cgagacggga tcctggacct cgaaatagag ggcgggctcc acgaggccgg
      601 ggaccagccg gggcttccag gcgaggccgg cggttaccga aggcaggacc aggacgctct
      661 ggctcgagca ggaggcgcgg ggaaagctcc cggcgccgag ctcggccagg agctcctcca
      721 ccgtcatctc ggagacgatg aaggaaggcg cgacgtcgcg gatcgagagg ccgaccgaga
      781 ggcttccgag ctcgacgctc gcgccgaagt ccatcgcgag gccgaagccc gactcgaccg
      841 actcgcccat gaagacggcg cccacgtcgc cgccgctcat gagcccgctc atgatctcgc
      901 cgaaggccca gccgccgtct gccgattccg cgcggaagaa gggccgcacg tcgccgccga
      961 gcgacacggt gaggccgccg atgcggatcg gcgcgcccag gcccaggacg gcgttgagcg
     1021 aggctgagct cgtcatcacg gcgcccatcg cggtctcgcc cttgacggag gaatcgccca
     1081 cgaggaacat gccgacgccc aggcccttgc ccacgtagcc ggcgccgaag gaaagcccgc
     1141 cgccgaagcc gttctccgtg atgaggtcgt tcatcgcgga gaggatctcc gcgtcgtcgg
     1201 ctccgcccga ggcgaggcgg gccgccttct ccaggttcgc gctcgtcggc ttcacgtaga
     1261 gccaggagga gaagtcggcc accatgagct cggccttggc cgcggcgaag cccgcggggt
     1321 tgccagacag ggcggagtag ccggaggaga gggcggtgaa ggccccgccc agggccatgc
     1381 tccgcgggtc cttggcggag agcggagaga gggctgctgt ctgggctccc gcgagagaag
     1441 cggccaggag aaccagggca aggacaaagg ttgtgcgctt catggtggtc tccatcctag
     1501 tacccgaatt tcgaaggatc caaacccgct tcggtaagca ggatattaag catactgcca
     1561 gacgcgagat cgggagcatt atacgtgctg ttaatatagc tagcagcatt tgatgaatcc
     1621 gttacggcgg accacagagc atcctctatc gtgccgctga cgggggacac gcccgccacg
     1681 gccgcggcaa cgaccgcgtt ttgagcgagg gttcccacac tataatcacc ggatacgcca
     1741 ctgccctcaa tgagagtttc gacacccgca ggattagtca agactgcatc ggcgcgaaga
     1801 agggcggcga tcgcagtctt gaatgccacc atatcggcac gaagctctgc aggaacaacc
     1861 agttcgacga gatcaactat ctcctcggga gtatcaatag aaggaaggtc gagacctccg
     1921 acgattgcag gaatgacctc ctttacaatg gcagaagccc cagaagtctc tatgagaatg
     1981 tccacagcta gagcagcggc ctcttgccta gccacaggcg cagctaagct atcattgtat
     2041 gtagtctgaa gggtggcgag gactacagtc tgcacggttg aatcataagg aagggcttcc
     2101 ataaaagtgg aggaggtgcc tccctcgaaa taggcagctt caaacagatc gtcggaggac
     2161 atttcagtta aatcggaagg cttcacctgc cccagtcccg cctccttgaa gaggttcgtg
     2221 gagaagaagg cgtcgcagga agcgaggatg aggaagcttc ccaccgcgag gacggcgagc
     2281 ttgggcttca taggaacctc cgaagctagg tttgactttt cgggattcgg tatacctaag
     2341 gctatcacat tttagagata tttcaaggag ggcggcggcc caaattgctc atttttccgg
     2401 tcaggaatgc aggaattccg cccttgcgac ggcgtcggcg agcggaagga cgccctcgcc
     2461 gctcccggct ttgaggcggc aacggatgcc cgcgcccggc ttcagggcgg gagcctcggg
     2521 caggccctcg acccgtagct tcaagtagtt ggccgaggtg gcgtagcgcg ccggccggcc
     2581 ttgggcgcgg ccaaggctgg cgctttccgc cgcatcgccc ccagccccgc cgacgacgac
     2641 agcgccttcg gccaaggcgc cttcctcctc gtcgcgctct cctccgctct ccaggacggc
     2701 gtccacctcg gcgccgagcc agcgctcgac gtaggccgcc ctccccttcc gcgcgagttc
     2761 gcccaaggcg gcggcccgct cgcccgcgat gcgctcgggc acgcgcccac tcatgtccca
     2821 ggccttcgtg ccgggcctcg gggagaaggg gaaggcgtgg atccaggcca ggtccgcggc
     2881 cttggccagc tcgagggtct gggcgaagtc ggcgtcggtc tccccgggaa agcccacgat
     2941 gaggtcggcg gccaggaaga ggtcgccctt ggcccgccgc aggccctcgc aggcggccag
     3001 gacggcgtcg cggcggtagg cgcggcccat ccgcgccagg acgggatcgg agccggattg
     3061 gatgggcagg tgggcgtggg gcctgacccg gggctcggcg aaggcggcga ggaaggcctc
     3121 gtccaccttg tcgggctcgt aggaggagac gcggagggct atcctctcgg tccccgaaag
     3181 gaggaggcgc aggaggccgg ggaagcccaa ggagccgtcg cggtactgag agaggttcac
     3241 gcccgtgagg acggcctcag cggcgccggc ctcctccagg gcgcgaaggc gggaaaggac
     3301 ctcggtcgcg ggaaggctcg tcgaggggcc gcgggccagg cagacccggc aataggtgca
     3361 gcggttgtcg cagccgtcct ggatcttgag agagggcctc gagtggaagc tgaaggactc
     3421 ggggcggaag gcgaagcggt cggccccgcc cgcgcggcca ccgccttcct ggccgccgga
     3481 ctctccctcg aggccgaggc gccattccaa caccgcgtcc aggaggcggc cgtggccctg
     3541 ccagtattcg gagagatggg aggcgaggcc caagagggcc gccttctcgt ctcccggcac
     3601 gacgacggcc cgttcgccca gggcctcgat cgccgccggt tccatctgcg cgtagcagcc
     3661 cgtcacgagg acgaccgcct cgggattgag ggccagggcc tggcggacca ggcgccgggc
     3721 cttctgctcg gccttgctcg tcacggtgca ggtgttgagg acgtagagct cggcctccga
     3781 ctcgaaggcg ccgatccggg cgccggcgcg gcggaaggcg tcggccaggg actcggactc
     3841 gagctggttg agcttgcagc ccagggtgcg gaaggcgacg cggacgtcca tcggctagcc
     3901 gcgggaacgg gcctggagac gctcggcgcc gagcctcgcg tcctcctggc ggggatcgag
     3961 gcgcagggcc tcggcgtagg ccgcgagggc gccctgggtc tcgccggcct tctcgcgggc
     4021 ccagcccagg cgggcccacc agcgggcgtt gcccggggcg aacttcaggg cggaggagag
     4081 ggctatgtcg gcgtgggcgt agcgggccat gcggaggtag atctcgccca tgtagtagta
     4141 ggccgtcccg acgcgggagc cctcgggcac ggcggagacg tagtcctgga agcgagagag
     4201 ggcggcctcg ttgcggccca gccggaaggc cgactcgccc aggatctccg tgacgcgggg
     4261 atccttccgg atcgcgtagg ccttgagcgc gtagttctcc gcgtccgccc agcgctcaag
     4321 cgcgaggagg ctccagcaga ggaccacgta ggactcgacg tcggcggggt cggcgcggag
     4381 ggcctccagc gagacgcggc gggcgtcctc gtgacggccc tcctggaaga gcttgagcgc
     4441 gtccccctcg gcggccgagg ccgcggcggc ggagcaaaga aggagggcga ggaggagggc
     4501 cgcgccgcgg gcggcggcga cgccgcgccg gccataggcg ccgaccgggc cgccgcgagc
     4561 gcggccgagc cggccgcggg tccggcgaga gtcccccgcg ggggagctag cgcgcatcga
     4621 agtcgggggc cgccatcttg caggcgccgt tgaagcggca gcccgtctcc atcgtgatct
     4681 ggggagcggt gacgtccccg tccaccgagc tcgaggcgaa gagctcgacc cgctcgcggg
     4741 cggcgatgtc gcccttcacg gagcccttca cggagaccac gagggccttg atgtcggcct
     4801 cgacgaccgc cttctcgtcg atgtagagct cgctctcgga gcggataacc ccgctcaccc
     4861 ggcccttgat catgaggggc ttcttgaagg tcatggagcc cgagaactcg acgtcgcccg
     4921 cgaggacggt gtcgagctgc tcctcgtcga ctgcgctgat ttggacttcg gtcatagctc
     4981 cgcatcatag ggcccggggg cccctgttgg caagggcgga accggcgcgg ccttgagccg
     5041 cccgtagacc gccgcgagct cctcgggcct cagggcctcg gcccggatcg agggggagaa
     5101 gcccagggcc tccagggcgg cggccacctc ggcctcgggc cggcccgcgg ccttcaggtt
     5161 gttcttgagg gtcttgcgcc gggaggagaa ggccgagcgc acgaaggccg agaagcccag
     5221 ccggtcggcg ggcgcgatcg ggtcgggccg gggctccagg accacgaggg agctctgcac
     5281 gttcggcacg ggccagaagg agccgccggc caggtcgaag gcgatacgga cgcggcaggc
     5341 cgaggcgcag agcacggaaa aggccgagta gtccttcgtg ccgggcttgg cggccatcct
     5401 gagggccgcc tccttctgca ccgtgaagac catcgggggc ggcccgcccg agcccgcggc
     5461 ctcggggtag cgctctatga ggagggcgac catggcgctc gcgacgttgt acggcagatt
     5521 gccgaagacg gcgtcgggca ggcgccccgc ggcgagttcc gtcttccagg tgcggaggaa
     5581 gtcgccctcc acgaggcgga acttgggccg tcccgcgaag atgcggccga ggagccgcgc
     5641 gaagccccgg tcgatctcga aggcggtgac ctccaggccc gagtccaggg ccatggccgt
     5701 catggagccg atgccggggc cgatctccca gatagccgcg cccccgccct ccgcgggagc
     5761 ggcgccggag ccggccgcgg cggcggttgg ggcggttgcg gcagcgggct tgccggcggc
     5821 atcagtgggc gcggacgtag ccgcgccggc cgcgtcctcg acggcggcct tgcctgcggc
     5881 ggcgactttt ccggcggcgc cagccttgcc ggcggcgccc gcggcgctct tcgggctggc
     5941 ggaaaggacc ttgcgaagct cctcgaggat gcgcgcgcgg gaggtcggat ttacgaggaa
     6001 gttctggccg aagcgcttcg acatcgcgag cccctccgcg tccaggaggg cgcgcaggga
     6061 ggccggggag tcgaacttgg gctcgagggg cttcgtcggg gcggcgctcg ccgggccgga
     6121 gccctcgagg gacggccccg ggacgctccc cggccgcgag ccttcggagc cagattcggt
     6181 gttcgacatg gggcagagca tacacgaagc aggcgccgag gacaacgaga gcggaagcca
     6241 gggccgcgcc ccgccggccg ggaagggcga gctcgggcca ggcggcgaaa cgggccatgg
     6301 tccccgagag gaggccgtgg aggagccgcg agacgggggc ggcgagggga gcgagggccg
     6361 gacagaagcc gcagaggagg gccgcggcca ggccccacca catgaaggcg gccacaagga
     6421 gggccgagag gacggaggcg aggaggccgg cgggatagag ggtcccgaag gtgaaggcga
     6481 ggaggggcgc cgtggccgcc tgggccgcga ggccggccgc cagggccttg gcgaggaagg
     6541 gagggaaaac cgggaggagg aggaactcga agcggggcgc gaggaggacg aggccggcca
     6601 gggccaggta ggagagctcg aaggagaggc tccgcgcggc cgcggggtcc aggggcaggg
     6661 cgacggcgaa ggccagcgcg aggatggtct gtcccggctg gggcctgtcc gcgaaaaaag
     6721 ccagggcccc gaggctgtac atgaggaggg agcggagaag ggaggggctc gcgccggcca
     6781 tgaagacgaa ggccagggcg aggaggaggg ccgccaggcg ggcctttctg ggtccgagga
     6841 ggggcttcgc gaggaaggtg gccagggccg cgaggatggc gaggtgctgg cctgaaaggg
     6901 ccaggacatg ggcgcagccc gcccgtttcc agtcgaccgc ctcctccagg tccaagccgc
     6961 cccggtcgcc catgaggagg gcgacaagaa ggcccgaggc ggcgccctcc ccgcccgcga
     7021 ggccaagcgc ggcgcggaag cgccggcgca ggctcgacct aagggcggcg acggggcctc
     7081 cgcccgtcca cgtctggagg ccgccggggt cggcgaagaa ggcgggcggg ccttgaggag
     7141 ccgggcttgc cgccggatcg ctcctttcgg ccggacgcgc ggcggatccg cccgctacgg
     7201 ccggacgcga ggcggacagg ccggaggcgg cagccgccgt ggcgccgccc ggccttcccc
     7261 gcgccgcgat cttcgcgccg gcctccagaa cctcgccgcc ccggaccagg aggcggcagg
     7321 ggcctgaggc ctccacccag cccgcgagtc ccggtccctc gaggagaagc gccgttagcg
     7381 agagtggata ggatcggaag ccgctttgcg agagggtcga gtccgcggag agccggccct
     7441 ccacgccgac gacggagaga ggccgagaag cagcccccgc ggccgatccg ccaacggcca
     7501 gccctgcggc ggcgccgata ggggcgccaa gcggcgggtg cggcgccgcg ggcgggcggg
     7561 ccgccgcggg aggaagaggc gaggcgggga ggaggggcgc gacggccgcg cccgcttcgt
     7621 ctcggcgcag ggccgcgccc aaggccaggc ccagggcggc ggcgaggcag aggatggcgc
     7681 ggagcctggg agcccgggcg cggaggccga ggaggaccga acccgcggcc gcggcggcca
     7741 tggcggcgac gaggacggcg ccgggcgtaa ggcggaggaa gtagaagccg aaggccaggc
     7801 ccaggccgag ggcccagggc ggggagaaga gccgaggctc ttcctcgagg aaaacaaaag
     7861 gcatcgagcc tccccggggg gcgggaaggc cgtggtcagc ctaccacctg aggcgcagga
     7921 gcgcttcctt cgcggcccgc ttcacctcgt ccgggtactt gaggtagccc acgtagagga
     7981 ggtagtcgaa ggccaccttg tcgcccagct cgccgagggc gcggatcgtc gcgaggatga
     8041 cctcgtcgtc gtaggagccg ccgagctcgg tctgcgcgtt gatgagctgg agctggaggg
     8101 ccagggcctg ggcggcctcg gaggagccca tcgcgcccag gcagacgatc gtctccagga
     8161 ggtcgcctcg cggcgcggcg cccttggcgt agtcggccag gagggcgcgg aaatagcgga
     8221 ggacgagggg cgaggcgcgc tgccagccga gctcgcggat cgtgcggatg gcgcggaggt
     8281 tgagctcgcg gatggcctgg ttctccacgg gcgcgccgcc cgtccaggcg aggccggccg
     8341 cgagggccgc ctccgcgagg ccgccccgct cctcggggcc gaaggcggga ttctcgagcg
     8401 cggccttgag ggccgccgcc ttctccaggg gggagctcat cgcgaggacc ctcgcgaggt
     8461 agcccgcgta gtcgcccttg atgcggccca gggccttgga ggccttcgcg gtcaggtcgg
     8521 ggttgtagcc cgccacgtag gtggagaaga ggacggggaa ggaggcgccg tcgccgatgc
     8581 gccccagggc gtccaccgcg gcctcgaggg ccgggtactc gggctgcatg ccggagcgga
     8641 agaccacgtt ctgcgaggcc aggaactccc cgaccgcggc gacgacctcc tcgttgcccg
     8701 cgcccgccgc ccccagggag gcgacggcca gggcgcggag ctcgacgtcg ccctcgtaga
     8761 ggcggaagag ggcccagagc ctggcggcgg agggagcgta ggccgcctcg ccggccttgg
     8821 cggcggcgat gaggacgagc tcgcggagct gggcgtcggc cgccccgagg aggggctcgc
     8881 tctccacggc gaagcgcagg gccgtgtcgt agaggggggc catctcctcc gcgttgcgcg
     8941 cgcccgcctc cttgaggagc tcgagcttcg cccccaggct ggagcgcacg aagttgcgct
     9001 ggtaggttag gaggacagag cccgcgtcct gggcgcgggc ctccggcggc gcgaaggcga
     9061 ggacaagggc gagcgcggcg accacggcgc ggactttcaa gagaggaccc ccttcatgac
     9121 acgcccgcgc cggcttcctc cgattcaacg gagagaggct cgagctcgcc ctcggcgcgg
     9181 atcttgtaga cgtaggagcg gatcgcgttg cgcgtccgcg ggctgggcgg agaggcctcc
     9241 acgtggagga cggagcggtt cttctcggag tcgtagtcga ccgagcgggc cacgcccgac
     9301 atcacgatct gcctgccctc caggccgaac tggaccttca cctggagacc cgccttgccc
     9361 cgccccccga tggcgacgga gaagccgtcc tcggagatgt cctccactcg gcagcgcagg
     9421 ccggggtcgc gctctggctt ctcgaaggcc ccctcgatgc gcttgaggag gtagaggtag
     9481 gcgggcaggc tcgtccggct ccgcaccgac ttccgcttct gcgttcgcag gagggactcc
     9541 gagtgcgcga cgtggatgac gggcacgttg cggatgcgca ggtcgtcgat ggcgtagctg
     9601 tcgaagacgt agcccgcgtc gtcgcggcgc cagaagtaga tggagacctt cttgcccttc
     9661 cacacgaagt tcttgggcag ccgcgcgccc gagggatagg agaggacgag gtagcggccg
     9721 ttgttgtcga tgacggtcga gccgtagacg ccgaggccgt ggacgaggac gcggaggcgc
     9781 tggccctggg agagctggcg cgaggagcgg atgccgctct tgtagcgcgg ctgctcgaac
     9841 tcgatccggc tccgcagggc gtagaccttg tccatgaggg cgatgccctc ccggctcctg
     9901 tcccgcccca ggtcccgctg cctcgcgagg aggacggcta tcgcgcggtc caggtcgcgc
     9961 ggggaggaga agaggctggc tggatcggcg atccggcaga gggacgccgc ctcgcggagg
    10021 atcctcgact cgccgtagcc gaagcctgag tccttggccc tgagctggaa ttccagccag
    10081 gaatggcgct gcccccccct gaccgcgagg accagggtga cgatggagag gacgatcacg
    10141 aggaggaaga gcgtcgtcat cggcatgctt cgttcgcttt tagaggtacc atacggcctt
    10201 ccatccatta tacaatgaaa tcgtcccgcg tcaaaacgct actgcgaggg tatacatgga
    10261 agaagtcgag gacaagggcc ccgc
//
LOCUS       DAOOSJ010000073        10210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4275_length_10210_cov_6.401083, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000073 DAOOSJ010000000
VERSION     DAOOSJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_10210_cov_6.401083"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLG14_05165"
     CDS             <1..652
                     /locus_tag="PLG14_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48558.1"
                     /translation="LSGDFAPAPGAADALGLVAARAAELRQASLSSKAEAERRLEREL
                     VAVKLKAFVETSRLARAERPELLFKAKLALERRLAAAAAPTASANASAGLIVAGTEAR
                     REGELALAPGPRGSIAAELLEAGLRIGIRFEPESPEALVAGFVEAGGERAVFGLGEAA
                     GGRTSGFPAFLSRHELARLYEGLCAAAAGLKAAAGGSGAEGASGRLSSALDSLLGL"
     gene            649..1245
                     /locus_tag="PLG14_05170"
     CDS             649..1245
                     /locus_tag="PLG14_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48559.1"
                     /translation="MSADFEAGFAQALAGKARSPLPRAYWVLPGLFLAGSYPGLPGLR
                     TEGPAALEPALSALLGAGVRRVVRLVEEREIGEGEDRLPSYDAILRALARERGFNLEI
                     LSRPIRDYGVPSREEMEGILDAIDESLGLGLPLYLHCWGGLGRTGTVVGCWIVRRGLA
                     RGGEALAALARLRAGAENASRPSPETAEQEDFVRSWGA"
     gene            1420..2400
                     /locus_tag="PLG14_05175"
     CDS             1420..2400
                     /locus_tag="PLG14_05175"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOX48560.1"
                     /translation="MADLSTCYLGLKLKNPLVVAASGLTASAEGARKAAAAGAGAIVL
                     KSLFEEQLDAELAGAEAALAQGAHPEAWDFLKGAGTEEGARDYLRLVAGAKEAGLPVI
                     ASLNCRGAGRWAEFAGRIEEAGADALELNMGFMPASTEELGEAVEERLFTALREVRAA
                     TKLPLALKLGSSYSSLPAVASEAARIGVQGLVLFNRFYRLDVDLAGLTLKAGASRSSG
                     SEYGESLRWISILADRVPCDLAASTGIHDAETALKLVAAGASAVQLCSVIYKRGFGVL
                     GEIARAMGDWMDGKGVASVAELKGRLAQWRSGDPAAYLRLQYIQALTGIS"
     gene            complement(2447..3661)
                     /locus_tag="PLG14_05180"
     CDS             complement(2447..3661)
                     /locus_tag="PLG14_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HOX48561.1"
                     /translation="MSAKDKDLSFAEATSIIAGYGIGGGVLALPYLVSLNGLLPSAAV
                     LVAAYCLSLLLHLMIAELSAGDGSGSQIVELFRKYLFTGKGGAALTWVFFAAMGAVFL
                     ANLAAYVAGGSEVLAAAGLPAPWGGLLFYAAAAAVAAFGLKVLGVAEKWAVGAMSLLF
                     AVLVAASLVALGKGGAGAGAAEAAATAAAATAAAALKAPRLLALYGMAMFCFAAFFSV
                     PQAVAGLAGRPKLVPKAVAAGLGVNFGLILVVTALSLLLSKEPTAIATVGWGRALGPW
                     AEAAGTAFVILAMLTSYWSISFALSSMLKERLGLGNFPAWLLATAPTLLLALAGLGGF
                     LDFMRTAGGAIAVLVAVLLVPAYRRYRKARAAAGQETPALLHPLFASPAWDWVAAVAY
                     LLMAVGSSLPIK"
     gene            complement(3658..5301)
                     /locus_tag="PLG14_05185"
     CDS             complement(3658..5301)
                     /locus_tag="PLG14_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX48562.1"
                     /translation="MQSAGTILKGRFYSLAREGERFAALRVGPDGRIAETYAAGAPLP
                     AGLPVRDLLAEGPREGRRPVAMVPAFIDSHVHFLPKAAMGALARPLARLEGGRLLPDC
                     LEGVGALLKEEAEAKPRGPVIGYGLFAGALAERRLPSAAELDTWVPGRPVLVFSLDGH
                     SSAYSSAALRLLKLESLAVEGRLSGEAHEFNMGRVMAYAMRGLGPAALARGIAGAVGE
                     AAAAGLASLHCLEGFDDAKSDPGVAALALVAPRLPLRLKLWLQYTEPRKVERYARLLG
                     NRRAGGCLAWEMDGSVSSRSAAFDRPYRDRGGSGSLYRSAEEAYALARPFFEAGYQLS
                     AHAIGPRGIESILSAYERLLEGTGEDAGGRRLRIDHFEFPRPDQVGRAGRLRLVLPVQ
                     PGFAWLDERVLHSYPEALDEEAARSFCPLKSLKEAGAVLALSTDAPVQPFDPFVQVAG
                     AVQHPNPAERLSVHEALRAYSWAGAYAAFEEGDRGTLEPGKYADFAVLDADPFGTPDD
                     KLSEIRALGTWHEGRPLAAPPGGLAGFALAALGAGRRRL"
     gene            5421..6836
                     /locus_tag="PLG14_05190"
     CDS             5421..6836
                     /locus_tag="PLG14_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48563.1"
                     /translation="MNKELRGTIGVMTLNQCLHWFMVGLVLPVLSLFVLEKGMDYLRL
                     GVLVAIYSGVTVAAELPTGGLADSLGRKKVYLLSLCCNALGALLLLAASAFPLLALGF
                     AVLGFGRALSSGSMEAHFVDEILALDPKADLQRIMARLGVAVPLALGFASLAGGWLPA
                     LGSRLQGLGLLSNKYAANYLAYLLAAFLQFVVTAVLVREARPVAPAASTATASTAPAA
                     FAKPAAVPSATPPSAKPPRATLSRVLKEALGFALRDRTLLLILSGGLVWGFSVSGLEQ
                     LWQPRVQELRGGVGSTAIFGWLGFGYFAAAAVGSWASTPLCRLFGGRHGLALGAAKLL
                     MGGFFLLLAGAGRVPAFAALYLLTFAANGLASAPESAVLHSSIPSERRSTLLSLSSLF
                     VQAGGILGSLLMGALAQAYSIGAAWRIAALVLAASSWLYFALPRRAGAVDSEGAASED
                     CASGAVGAAAGAQAAAEGEGA"
     gene            6833..7630
                     /locus_tag="PLG14_05195"
     CDS             6833..7630
                     /locus_tag="PLG14_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX48564.1"
                     /translation="MSFAEIGDRRSLSDKVLEAVTQAILSGEWAEGDLMPAEPELAAQ
                     FGVSRSVIRDAARMLVAKGLVEIRRGKGMYVASSPSKAFAEALVLALRRRRASAWDVE
                     EFYSLFLPEIFALAARKATAGQKVGIREAAEAYVAAYAELSGKPETEEGIFLGKRLFA
                     AFARRVLEVTGNELCLLLGEALVQLRGFREVDTGPEGSGSEASRRLVKSESRIMRGLA
                     EAVAGGDPEAARASVAAYLRATPAASKRLEETPVGEIPKLTLDMLGS"
     gene            complement(7710..8054)
                     /locus_tag="PLG14_05200"
     CDS             complement(7710..8054)
                     /locus_tag="PLG14_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48565.1"
                     /translation="MEEQRKKGGAEAEGSAPAASGAAPACGAFAGVEMEFPVTFDLKI
                     IYVLAEGGTIVEDLERIYAALGVKCAMIQGVAAPGAKYGRMGSRLTFETREQMYAAYA
                     AIGKLPYVKTAI"
     gene            8103..>8880
                     /locus_tag="PLG14_05205"
     CDS             8103..>8880
                     /locus_tag="PLG14_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX48566.1"
                     /translation="MQETIPSFAVVGAGDRGTGYAAYTEAACRRAAGLPEGPGARVAA
                     VAEPDPVRRERFADRFGLPASARFPGWKELFAGPRLAEGVVIATRDGEHVEPALAALE
                     AGYAVLLEKPMALSEEDCRRLAAASEAAGLPLLVCHVLRYHPFFKAVRGLVEEGAIGD
                     LVSVRQAENVSYWHMAHSYVRGNWRDASASPMILAKCCHDLDLLCWMAGPSARGAAAA
                     DGASSARGCAAKPLRVASFGSNLEFRPERAPAGSPQRCVEG"
     assembly_gap    8881..8980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9094..10209
                     /locus_tag="PLG14_05210"
     CDS             9094..10209
                     /locus_tag="PLG14_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOX48567.1"
                     /translation="MIDQRLVKLARILVNYSTKVKPGEKVLIQDNNVEADFVAALVEA
                     VHDAGGLPFVTLRDKRVERALFRRATEEQMALQAKFERERMAEMDCYIGFTSIRNLSS
                     WQDLPADKLELYNRHVWKKVHIDTRLPKTRWVVLRYPSPAFAQMAGMSEEAFEDWYFD
                     VCTMDYARMSKAMDPLVDLLSRTDRVRLVAPGTDLSFSVKGLPPVKCDGGLNIPDGEV
                     YTAPVRDSVEGRITYNTPSEKDGFCYEGISFEFKGGRIVKASANDTARINQVLDIDEG
                     ARYVGEFAIGVNPFVTKPMKETLFDEKISGSIHFTPGNSYDDCFNGNRSSLHWDLVLI
                     QTPEWGGGEIWFDGRLVRKDGRFVVPELEGLNPERLK"
BASE COUNT         1364 a         3679 c         3632 g         1435 t
ORIGIN      
        1 cctctcgggc gatttcgccc ccgcgcccgg agcggccgac gccctgggcc tcgtcgcggc
       61 cagggccgcc gagctcaggc aggcgagcct ctcgtccaag gccgaggcgg agcggagact
      121 cgagcgcgag ctcgtcgccg tcaagctcaa ggccttcgtg gagacctcgc gcctggcccg
      181 cgccgagcga cccgagctcc tcttcaaggc caagctggcc ctggagcgcc gcctcgcggc
      241 ggccgcggcg ccgacggcgt cggccaacgc gtccgccggc cttatcgtcg cgggaaccga
      301 ggcgaggcgc gagggcgagc tcgccctcgc gcccgggccg cgcggctcga tcgcggccga
      361 gctcctggag gccggcctca ggataggcat ccgcttcgag cccgaatcgc ccgaggccct
      421 cgtcgcgggc ttcgtcgagg cgggtggaga gcgggccgtc ttcggcctgg gcgaggcggc
      481 cggcggccgg acaagcggct tcccggcctt cctctcgcgc cacgagctcg cgcggctcta
      541 cgaagggctt tgcgcggcgg ccgctggcct gaaggccgct gccgggggct cgggcgccga
      601 gggcgcttcg ggccggctct cctcggccct cgactccctc ctcggcctat gagcgccgac
      661 ttcgaagcgg gcttcgccca agccctggcg gggaaggcgc gctcgcccct tccccgcgcc
      721 tactgggtcc tcccgggcct cttcctggcc ggctcctacc ccggcctgcc aggcctcagg
      781 acggagggtc cggccgcctt ggagccggcg ctcagcgccc tcctcggcgc cggcgtcagg
      841 cgcgtcgtcc ggctcgtgga ggagcgggag atcggcgagg gcgaggacag gctgccttcc
      901 tacgacgcca ttcttcgcgc cctcgcccgc gagcggggtt tcaacctgga gatcctctcg
      961 cggcccatcc gcgactacgg cgtcccctcc cgcgaggaga tggagggcat cctggacgcg
     1021 atcgacgaga gcctcgggct cggactcccc ctctacctcc actgctgggg cggactcggg
     1081 cggacgggca ccgtcgtcgg ctgctggatt gtccgacgcg gcctcgcgag gggaggggag
     1141 gccctggccg ccctcgcccg tctccgcgcc ggcgcggaga acgcctcccg cccctcgccc
     1201 gagaccgccg agcaggaaga cttcgtccgc tcctggggcg cctagcccgg gccgtggtgg
     1261 ccgggccttc gcggcgggcc gtgggggacg cagcgcccgg tccgcgccgg ccgccccaag
     1321 gggcagggcc ctcgccgtcc ggacttcctt gcctccgcgg ccttccggaa ctagatttct
     1381 agacgacagg catcgtcagg tcttcaggaa ggagtcgcca tggcggatct atccacctgc
     1441 tatctaggtc tcaagctcaa gaaccccctc gtcgtcgccg cctcgggcct gacggcgagc
     1501 gccgagggcg cgcgcaaggc cgccgccgcc ggagcggggg cgatagtcct caagtccctc
     1561 ttcgaggagc agctcgacgc cgagctcgcg ggggcggagg cagccctggc ccagggcgcc
     1621 catcccgagg cctgggactt cctgaagggg gcgggcactg aggagggcgc gagggactac
     1681 ctccgcctgg tcgccggagc caaggaggcg ggccttcccg tcatcgcgag cctcaactgc
     1741 cgcggcgcgg gccgttgggc cgagttcgcg ggcaggatcg aggaggccgg cgccgacgcc
     1801 ctcgagctca acatgggctt catgcccgcc tccacggagg agctgggcga ggcggtggag
     1861 gagcggcttt tcaccgcgct gcgcgaggtc agggccgcga cgaagctgcc cctcgccctc
     1921 aagctcggct cctcctattc gagcctgccc gccgtcgcct ccgaggcggc gcggataggc
     1981 gtgcagggcc tcgtcctctt caaccgcttc taccgcctgg acgtggacct cgcgggcctc
     2041 accctcaagg ccggggcctc gcggagctcg ggctcggagt acggcgagag cctgcgctgg
     2101 atctccatcc tcgcggaccg cgtgccctgc gacctcgcgg cctccacggg gatccacgac
     2161 gcggagacgg ccttgaagct cgtggccgcg ggcgcctccg cggtccagct ctgctcggtc
     2221 atctacaagc gcggcttcgg cgtcctgggg gagatcgcga gggcgatggg cgactggatg
     2281 gacgggaagg gcgtcgcgtc cgtcgccgag ctcaagggcc gcctggccca gtggcgctcg
     2341 ggcgatcccg cggcctacct ccggctccag tacatccagg ccctgaccgg gatctcctga
     2401 ggcgtccagc ccggccggcg ggccgcggcc tgccggccga gagcccctac ttgatcggca
     2461 gcgaggatcc gacggccatg aggagatagg cgacggcggc gacccagtcc caggcgggcg
     2521 aggcgaagag ggggtggagg agggcgggcg tttcctggcc cgccgccgcc ctcgccttcc
     2581 tgtagcggcg gtaggcgggc acgaggagga cggcgacgag gacggctatc gcgccgccgg
     2641 ccgtgcgcat gaagtcgagg aagcctccga ggcccgctag ggcgaggagg agggtcggcg
     2701 ccgtcgcgag gagccaggcg gggaagttgc cgaggccgag cctctccttc agcatcgagg
     2761 agagggcgaa ggaaatcgac cagtagctcg tgagcatggc gaggatcacg aaggccgtgc
     2821 ccgccgcctc cgcccaaggg cccagggccc tgccccagcc gaccgtcgcg atggccgtgg
     2881 gctccttcga gaggaggagg gagagggccg tcacgacgag gatgaggccg aagttgacgc
     2941 cgagtccggc cgcgacggcc ttggggacga gcttgggcct ccccgcgagg cccgcgacgg
     3001 cctgcggcac ggagaagaag gccgcgaagc agaacatcgc catgccgtag agggcgagga
     3061 ggcgcggagc cttgagggcc gcggccgccg tggctgcggc cgccgtggcc gcggcctccg
     3121 cggcgcccgc tcccgcgccg cccttgccga gcgcgacgag agaggccgcg accaggacgg
     3181 cgaagaggag gctcatcgcg ccgacggccc atttctccgc gacgccgagg accttgagcc
     3241 cgaaggccgc gaccgccgcg gccgcggcgt agaagaggag tccgccccag ggcgcgggca
     3301 ggcccgcggc cgcgagtacc tcgctcccgc cggcgacgta ggcggccagg ttcgcgagga
     3361 agacggcccc catggcggcg aagaagaccc aggtcagggc ggcgcctccc ttgcccgtga
     3421 agaggtactt ccggaagagc tcgacgatct ggcttccgct cccgtcgccc gcggagagct
     3481 ccgcgatcat gaggtggagg aggagggaga ggcagtaggc cgcgacgagg acggccgccg
     3541 agggcaggag cccgttcagg gagacgaggt agggcagggc gagcactccc ccgccgatgc
     3601 cgtagcccgc gatgatgctc gtcgcctcgg cgaagctcag gtccttgtcc ttcgcgctca
     3661 cagcctcctc cttcccgcgc cgagcgcggc cagggcaaag cccgcgaggc ccccgggggg
     3721 cgccgcgagc ggccgcccct cgtgccaggt cccgagggcc cgtatttcgc tcaacttgtc
     3781 gtccggcgtc ccgaaggggt cggcgtccag gaccgcgaag tccgcgtact tccccggctc
     3841 cagggtgccg cggtccccct cctcgaaggc ggcgtaggcc cccgcccagg agtaggccct
     3901 gagggcctcg tggactgaga ggcgctccgc ggggttcggg tgctggaccg cgcccgcgac
     3961 ctggacgaag gggtcgaagg gctggacggg cgcgtccgtg gagagggcga ggacggcgcc
     4021 ggcctccttg agggacttga gggggcagaa ggacctggcg gcctcctcgt ccagggcctc
     4081 cgggtaggag tggaggaccc gctcgtcgag ccaggcgaag cccggctgga cggggaggac
     4141 gaggcggagg cggccggccc gcccgacctg gtcgggcctg gggaactcga agtggtcgat
     4201 ccttaggcgg cgcccgcccg catcctcccc ggtcccctcg aggaggcgct cgtaggccga
     4261 gaggatggac tcgatgccgc ggggcccgat cgcgtgggcg gagagctggt agccggcctc
     4321 gaagaagggc cgggccaggg cgtaggcctc ctcggcgctc cggtagaggc tgcccgagcc
     4381 gccgcggtcg cggtaggggc ggtcgaaggc cgcgctccgc gaggagaccg agccgtccat
     4441 ctcccaggcc aggcagccgc ccgccctgcg gttccccagg agccgcgcgt agcgctcgac
     4501 tttccggggc tccgtgtact ggagccagag cttgaggcgc aggggaaggc gcggcgcgac
     4561 gagcgcgagc gcggcgactc cgggatcgct cttcgcgtcg tcgaagccct ccaggcagtg
     4621 gagggatgca agccccgccg ccgcggcctc gccgaccgcg cccgcgatcc cgcgggccag
     4681 ggcggccggc cccaggccgc gcatcgcgta ggccatcacg cggcccatgt tgaactcgtg
     4741 ggcctcgccg gagagcctac cctcgacggc cagggactcg agcttgagga ggcgcagggc
     4801 ggcgctcgaa taggcgcttg agtggccgtc gagcgagaag acgaggacgg ggcggccggg
     4861 gacccatgtg tcaagctcgg cggcggaggg gaggcggcgc tcggccaggg cccccgcgaa
     4921 gaggccgtag ccgatgacgg ggccgcgggg cttcgcctcg gcctcctcct tgaggagggc
     4981 gcccacgcct tccaggcagt cgggcaggag gcgcccgccc tcgagccggg cgaggggccg
     5041 ggccagcgcg cccatcgccg ccttgggcag gaagtggaca tggctgtcga tgaaggcggg
     5101 gaccatggcg acgggacggc gcccctcgcg cgggccctca gccaggagat cgcggacggg
     5161 caggcccgcg gggaggggcg cgcccgcggc gtaggtctcg gcgatccggc cgtcggggcc
     5221 gacgcgcagg gccgcgaagc gttcgccttc cctggcgagg ctgtagaagc ggcctttcag
     5281 gatggtgcct gcggattgca tgaaccgtag tatgggtgcc cggccccgcc ggcgcaagcg
     5341 cgtatcttcc ttcggccttg acggaaggcg gaagacgagt tatcttgaag taagacgtat
     5401 gatgtcataa gaggtgagcg atgaacaagg aactccgcgg gacgatcggc gtcatgaccc
     5461 tcaaccagtg cctgcattgg ttcatggtcg gcctcgtcct ccccgtcctc tccctcttcg
     5521 tcctcgagaa gggcatggac tacctgcggc tcggcgtcct tgtcgcgatc tattcgggcg
     5581 tcacggtcgc cgccgagctg cccacgggcg gcctcgccga cagcctgggc cggaagaagg
     5641 tctacctgct ctcactctgc tgcaacgcgc tgggagcgct cctcctcctg gccgcgagcg
     5701 ccttccccct cctcgccctc ggcttcgcgg tcctcggctt cggacgggcc ctctcctcgg
     5761 gctcgatgga ggcgcatttc gtggacgaga tcctcgccct cgaccccaag gcggacctgc
     5821 agcggatcat ggcgcggctc ggcgtcgccg tccccctcgc cctgggcttc gcctccctgg
     5881 ccggcggctg gctgccggcc ctgggctccc gcctccaggg gctcggtctt ctgtccaaca
     5941 agtacgcggc caactacctc gcctacctcc tggcggcatt cctccagttc gtcgtgacgg
     6001 ccgtcctggt gcgcgaggcc cggcccgtgg cgcccgccgc ctccacggcg accgcctcca
     6061 cggcccccgc cgctttcgcc aagcctgcgg ccgtgccgag cgcgacgccg ccgagcgcga
     6121 agccgccgcg cgcgacactg tcgcgagtcc tcaaggaggc gctgggcttc gccctccggg
     6181 acaggaccct cctcctcatc ctctcaggcg gcctggtctg gggcttctcc gtgtcggggc
     6241 tcgagcagct ttggcagccc cgcgtccagg agcttcgcgg cggagtcgga agcaccgcca
     6301 tcttcggctg gctgggcttc ggctatttcg cggcggcggc cgtcggcagc tgggcctcga
     6361 cgcccctctg ccgcctcttc gggggccgcc acggcctggc cttaggggca gccaagcttc
     6421 tcatgggggg cttcttcctc ctcctcgcgg gcgcggggag ggtgcccgcc ttcgccgccc
     6481 tctacctcct gaccttcgcg gccaacggcc tcgcctcggc ccccgagagc gccgtcctcc
     6541 actcctccat cccctccgag cggcgctcga ccctcctctc cctgtcctcc ctcttcgtcc
     6601 aggcgggcgg catcctcggc tccctcctca tgggcgccct cgcgcaggct tattcgatag
     6661 gggcggcttg gcgcatcgcg gcgctggtcc tggccgcctc gtcctggcta tacttcgcac
     6721 tgccgcgccg cgccggggcc gtcgactccg agggggccgc ctcggaggat tgcgcctctg
     6781 gcgccgtcgg cgccgcggcc ggcgcgcagg ccgccgcgga gggggaggga gcatgagctt
     6841 cgccgagata ggcgacaggc ggagcctgtc ggacaaggtg ctggaggccg tcacgcaggc
     6901 catcctgtcg ggcgagtggg ccgagggcga cctcatgccg gccgagcccg agctggccgc
     6961 ccagttcggc gtctcgcggt cggtcatcag ggacgcggcg cgcatgctcg tggccaaggg
     7021 ccttgtcgag atccggcgag ggaaggggat gtacgtcgcc tcctcgccct ccaaggcctt
     7081 cgccgaggcc ctggtcctcg cgctgaggcg gcgcagggcg agcgcctggg acgtcgagga
     7141 gttctactcc ctcttcctcc ccgagatctt cgccctcgcg gcccggaagg ccacggccgg
     7201 acagaaggtg gggatcaggg aagcggccga ggcctacgtc gccgcctacg ccgagctctc
     7261 cggaaagccc gagaccgagg aagggatctt cctagggaag cggctcttcg ccgccttcgc
     7321 gcggcgggtc ctcgaggtca cgggcaacga gctctgcctc ctcctgggcg aggccctcgt
     7381 ccagctccga ggcttcaggg aggtcgacac gggcccggaa ggctcaggct cggaggcgtc
     7441 gaggcggctc gtgaagtcgg agtcgaggat catgcgcggc ctggcagagg ctgtcgccgg
     7501 cggggatccc gaggccgcga gggcgagcgt cgcggcctac ctccgcgcga cgcccgcggc
     7561 cagtaagcgt ctcgaggaga cgcccgtggg cgagatcccc aagctcacgc tggacatgct
     7621 gggctcttga tccaggcgct tggccgctcc ggaccgcgcc gcggccgctg cgaggcagtc
     7681 gtgccggacg cgcctcgatc cgtcccaggc tagatggccg tcttcacgta ggggagcttg
     7741 ccgatggcgg cgtaggccgc gtacatctgc tcgcgcgtct cgaaggtgag gcggctcccc
     7801 atgcggccgt acttggcgcc cggggccgcg acgccctgga tcatcgcgca cttcacgcca
     7861 agcgcggcgt agatccgctc caggtcctcg acgatggtcc cgccctcggc gaggacgtag
     7921 atgatcttga ggtcgaaggt gacggggaac tccatctcga cgcccgcgaa ggcgccgcag
     7981 gccggggccg ctccgcttgc cgcgggagcc gagccttcgg cctccgctcc cccttttttc
     8041 cgctgctctt ccatgctttc atgatgccac ggcgcgatcg cgcctgtcga ggaggccgca
     8101 tcatgcagga gacgatcccg tccttcgccg tcgtgggcgc gggcgaccgg ggcacgggct
     8161 acgcggccta caccgaggcc gcctgccgcc gcgccgcggg gctcccggag gggcccgggg
     8221 cccgcgtcgc cgcggtcgcg gagccagacc ccgttaggcg ggagcgcttc gccgatcgct
     8281 tcggccttcc cgcctccgcc cgcttcccgg gctggaagga gctcttcgcg gggccgcgcc
     8341 tggccgaggg cgtcgtcatc gcgacgcgcg acggcgagca cgtcgagccc gccctggcgg
     8401 ccctggaggc gggttacgcc gtcctcctgg agaagcccat ggccctctcc gaggaggact
     8461 gccgccgcct cgccgccgcc tccgaagccg cgggcctgcc cctcctcgtc tgccacgtcc
     8521 tccgctacca tcccttcttc aaggccgtcc gcggactcgt ggaggagggc gcgataggcg
     8581 acctcgtctc cgtcaggcag gcggagaacg tctcctattg gcacatggcc cactcctacg
     8641 tcagggggaa ctggcgcgac gcctcggcct cgccgatgat cctcgccaag tgctgccacg
     8701 acctcgacct cctctgctgg atggccggcc catccgcgcg aggcgccgcg gcggcggacg
     8761 gcgcctcctc cgcgcgcgga tgcgccgcga agcccctccg cgtcgcctcc ttcggctcca
     8821 acctggagtt ccggcccgag cgcgcgcccg cgggctctcc ccaaagatgc gtcgagggct
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgcgccca agcgccgctc
     9001 cacatcgttt tgtggagcga aacggcgctt cgatttcctc ttcacggccc gcgattcccg
     9061 ggataaaatc ccgttcgcga caaggaggct ctcatgattg accagcggct cgtcaagctc
     9121 gcccgcatcc tcgtgaacta ttccacgaag gtgaaaccgg gcgagaaggt cctcatccag
     9181 gacaacaacg tggaggccga cttcgtcgcc gccctcgtgg aggccgtcca cgacgccggc
     9241 ggccttccct tcgtcacgct ccgggacaag cgggtggagc gtgccctctt ccgccgggcc
     9301 acggaggagc agatggccct ccaggcgaag ttcgagcgcg agcgcatggc cgagatggac
     9361 tgctacatcg gcttcacctc gatccggaac ctctcctcct ggcaggacct ccccgcggac
     9421 aagctcgagc tctacaacag gcacgtctgg aagaaggtcc acatcgacac ccgcctcccc
     9481 aagacccgct gggtggtcct ccgctatccc tcgccggcct tcgcgcagat ggcgggcatg
     9541 agcgaggagg ccttcgagga ctggtacttc gacgtctgca cgatggacta cgcgaggatg
     9601 tcgaaggcca tggaccccct cgtcgacctc ctctcgcgga cggaccgcgt gcgcctggtc
     9661 gcgccgggca ccgacctctc cttctcggtg aagggcctgc cgcccgtgaa gtgcgacggc
     9721 ggcctcaaca tccccgacgg cgaggtctac accgcgccgg tccgcgactc ggtggagggc
     9781 cgcataacgt acaacacgcc gagcgagaag gacggcttct gctacgaggg catctccttc
     9841 gagttcaagg gcggcaggat cgtgaaggcg agcgcgaacg acaccgcgcg catcaaccag
     9901 gtcctggaca tcgacgaggg cgcccgctac gtcggcgagt tcgcgatcgg cgtcaatccc
     9961 ttcgtcacga agccgatgaa ggagaccctc ttcgacgaga agatctcggg ctcgatccac
    10021 ttcacgcccg gcaactccta cgacgactgc ttcaacggca accgctcctc cctccactgg
    10081 gacctcgtcc tcatccagac ccccgagtgg ggcggcggcg agatctggtt cgacggccgc
    10141 ctcgtgcgca aggacggccg cttcgtggtc cccgagctcg agggcctgaa ccccgagcgc
    10201 ctgaagtagc
//
LOCUS       DAOOSJ010000074        10156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4304_length_10156_cov_6.614296, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000074 DAOOSJ010000000
VERSION     DAOOSJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4304_length_10156_cov_6.614296"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..429
                     /locus_tag="PLG14_05215"
     CDS             16..429
                     /locus_tag="PLG14_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HOX48568.1"
                     /translation="MEERYFVVKAEMPKGEAIKQALAKTYKFYETLDAWADDCVKEWK
                     YYNRRYGWSLKVSWRKKPLYWVVMYRGFFHMGFSIAKNERDDFLALDLEEATRRAITD
                     SKEFPEGFPVEFAVRDEKSFQEAFRLVKKQRELLG"
     gene            complement(426..1157)
                     /locus_tag="PLG14_05220"
     CDS             complement(426..1157)
                     /locus_tag="PLG14_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOX48569.1"
                     /translation="MQTDIEAIAGHFAGEAIIGEVEGILKSGKEAVVYRCTAHPSTGL
                     STVALKVYKDIERRGFRDMSDYLDGRVGRTIRKRRDILHMYSSPDSMQAYWVDAEYEA
                     LERLHAAGLPVPRPLGRGPASIAMEFVGGEEAAPRLRDARLGPGEAQDVKEELVECVR
                     RMLELDLIHGDLSPYNILMREGRPVIIDFPQAIDARFHSRPQAMLERDLSNVLSHFER
                     YGLGSAAEGAALAAEWWGRYERNGL"
     gene            1331..4174
                     /locus_tag="PLG14_05225"
     CDS             1331..4174
                     /locus_tag="PLG14_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48570.1"
                     /translation="MKKPNLKRYYQNLVNALSFSSELYKLRRELNTKIDIPDAVVPTT
                     HSHPLGVTRWFKKRRISIAEAYLMVIRDLESKQSKARLRSLRMMVEASFHTKSLDLPL
                     NTARVQMALIKEAVKNRGNRRRQLELLQDFSVSSHGQYQVIRKLCDELNIIELPETGE
                     PLKRLDAGWDEHVHDTATSGRKNATQLLIDAFIKGISELTISYGSAVSLDMMEEAVEA
                     GRIVGVRVKIGLEFSMMAQGKRFHFMALLPPFKGAKDVERWFKEHAEGLKELFDGLEK
                     NQENRIEAVRSLLKNFNKTSLKELNEGYPDDRLYAVPKLKMKKLSAFIPLSSINRMHL
                     GEFLYSYYKPVLLNRILLLKVQREKARCDLRKRLISDWDFRIIDERYTRLRDEYRELN
                     PDNLRKQYFTNPKIGDYQTVFDDMEAIKASLSRAGCRLKILHPLEYGLDKAKKLLSKS
                     RGLIDEVEIYNMQDSVLRDPDEILKLARYINDLNLESVRDGSPAYVPVCGSDSTGRSP
                     KIPGMGFIRQDRIGGKYRRRYIRRHVALPSLVSAMVEAEGTHVTQEMVAAAPVILSMG
                     KISGGSANLIGDEPENESLEIGPARAWRYLNPTLVNLAYAAVGFLVADRFIGPFYAFL
                     WLFITGFRNSIADLVASRGTRLAEWNWKSVNFGNVARSLFWTGFSVPILGFVKARFDL
                     AWPWAPEGPLFDSAKFFFISFSNGLYLAAHNTLRGFDRKVVKANFFRSVIAWPFATAF
                     SPFGSLLGIPSIVQAKIWSDFVAGFIEGGGKYMKVLRLRRRDLEDIVPRIISGDQEDR
                     FTALLDLLFLYREEPRTESSLKAVMDPGYRPMGILKDDKDAPKYSFSELYAAVMDESI
                     DRPLVDFVLSRYGHDVAVDLAGLVALTLPAMREWFEAHAARFLAPQRQPAAAAREAEP
                     GVQPAAAAPVAAPVAAKKDAPAALPEAGAKARD"
     gene            complement(4184..5317)
                     /locus_tag="PLG14_05230"
     CDS             complement(4184..5317)
                     /locus_tag="PLG14_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX48571.1"
                     /translation="MIPAAGGAGKGVPARQAVLIIDDDAKNLALLSEYLGGYDLAILV
                     AEDGRSGVERARYARPDLVLLDVVLPDGDGYGICRRLKADPETAETPVIFMTALSETE
                     FKVKGFEAGGVDYITKPFRREEVLARVGVHLRLAELSRGLREANEGLERRVEERTAEL
                     AEANLDLRRLLGERDALLKEVHHRVKNNLQVIVSLLGLQAGAEDDARALAALERFRAR
                     VLSMALVHESLYRSQDFRHVDFGAIASELAAALRSDRDHPGRAAAEIAVDLGELSLPV
                     DTALPLALALGECVSNSLEHAFPPGWEGPRKVSIAGGREGPRCAITIADSGIGLPDGF
                     DPAAGESLGMRIAAAAMSQVGGRLAPGSGPGAALRLEFELPEP"
     gene            complement(5314..8274)
                     /locus_tag="PLG14_05235"
     CDS             complement(5314..8274)
                     /locus_tag="PLG14_05235"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOX48572.1"
                     /translation="MPLVDEIVSLVGGSATVFQRAGEDGDMLRVATTVLDSEGKRAVG
                     TYIPAREPDGMPNAVVAAALAGRTYLGRAYVVNDWYLTAYEPIQDASGRTIGMLYVGT
                     IQKSAEARIREAILDLRLGKTGYVFVITGSGEDRGRYVISQGGTRDGEYVWDVRDSDG
                     RYVTHEIVEQGMALGPGEKTTIRYEWRNPGEAAPRWKVARLAYYEPWDWVIGASVYED
                     ELEGYRAVLQRGSAGMAATMAVSGLAMTLVVGLAGIALARSITGPVRKVTRAAEVLMA
                     GGSAERVEVGSRDELGTLARTFNLMSERIGATLEGLRESENKYRGIYENALEGIFRTS
                     VSGRLLRANPSLARILGYDSPEELVAGLGDVRSQLYVRGSDRDGFLADLKARGAAIGK
                     EVLLRRKDGSAIWVSLSARAVPDASGGMAFIDGFIADIDALKKAEEELKRGREHLEDL
                     VAERTAELVAAKETAESASRAKSEFLANMSHELRTPLNGIIGYAQILKRNGGLSASQD
                     FAVGTIERSGEHLLTLINDILDISRIEAKRMTLHPTALDLGAFLSGVAGIAATRAEAK
                     GIGFAYLPGEGLPAAVEADETRLRQVLLNLLGNAVKYTQSGEVRFSVSTAGPRGGTGS
                     AGPGDARPPGDGKGGKARLRFEVADTGIGLASEDLARVFEPFFRAGSSAASAEGAGLG
                     LPISRALVRAMGGDIEIRSEPGRGSSFWFELELPLGSRAEGAAPDWEEGISGYAGPRR
                     TVLVADDSPDNRSVLRDMLAPLGFRILEAEDGAKALELALAERPDLVLMDLRMPGTGG
                     IEAARRIKAGTGPEPVHVIAVSASAFPEDLLMSAGAGCEAFVRKPVRIGDLLKAMGGA
                     LGLEWLRAAAKPAHAAPHHAARPEAFPGAPVGGGSGASASAALEADAGAAAEAPPEAE
                     AGVPPGFSQAALRELLELARIGDMRGVAAWAEGMAKDRPEARRHADRLRALAAEFRAK
                     SILALAEEWMEGRT"
     gene            complement(8253..8783)
                     /locus_tag="PLG14_05240"
     CDS             complement(8253..8783)
                     /locus_tag="PLG14_05240"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOX48573.1"
                     /translation="MAASPGTYGGRKASGLGGFEGAQGSECGEATPVGGFQWLRRLDA
                     KISLLAAGSAFVAALALVLLAAALSSDYNALAQREVDSLVDEDLGHVTTSAYNLVRAE
                     DEAVRQAVASSLNVARRMLGRSGGISESREKVSWSAINQFSGSVEEFVLPRVLLGGRA
                     LPTRPNRGGPCPSSTR"
     gene            complement(8975..9490)
                     /locus_tag="PLG14_05245"
     CDS             complement(8975..9490)
                     /locus_tag="PLG14_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48574.1"
                     /translation="MKKHTATFLSLALVALLVLPAALGAEPLLTDAFLGTLLRYCREA
                     AATLPPEKRLEAATLRFNTGREMTVLEAEQVKRFADVYCADMLPELSGGMGAAEMGGA
                     VANEAMAAAREEILADPDLDEATKASLLAAVDAELGAMLGRLGEMETADPADLALFRK
                     YRADFDAVLKP"
     gene            complement(9600..>10156)
                     /locus_tag="PLG14_05250"
     CDS             complement(9600..>10156)
                     /locus_tag="PLG14_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOX48575.1"
                     /translation="ELEGVHNRTDFDLKRHQEFSGKDLQYIDQDNGNERYIPYIIETS
                     AGLTRQLLMILCDAYEEQKVADKGNDDDWRTVMRFHPNLAPVTVAVLPLMKKDGLAEL
                     AQEIRTGLKEEFATDYDQSGAIGRRYRRQDEIGTPFCVTVDYQTKEDGTVTVRFRDSM
                     EQVRIHRDELESTLKKAIKDYKRV"
BASE COUNT         1541 a         3596 c         3441 g         1578 t
ORIGIN      
        1 ccgaggacca aagccgtgga agagagatat ttcgtcgtca aggcggagat gcccaagggc
       61 gaggccatca agcaggccct cgccaagacc tataagttct acgagaccct cgacgcctgg
      121 gcagacgact gcgtcaagga atggaagtac tacaaccgcc gctacggctg gtccctcaag
      181 gtctcgtgga ggaagaagcc cctgtactgg gtcgtcatgt accggggctt cttccacatg
      241 ggcttctcga tcgccaagaa cgagcgggac gacttcctcg ccctcgacct cgaggaggcg
      301 acgcgcaggg ccatcaccga ctccaaggag tttcccgagg gcttccccgt ggagttcgcg
      361 gtgcgggacg aaaagtcctt ccaggaggcc ttccgcctcg tcaagaagca gcgggagctc
      421 ctgggctaga ggccgttcct ctcgtagcgt ccccaccact ccgcggccag ggccgcgcct
      481 tcggccgcgg agccgaggcc gtagcgctcg aagtgcgaga gcacgttcga caggtcgcgc
      541 tcgagcatgg cctggggcct cgaatggaag cgggcgtcga tggcctgcgg aaagtcgatg
      601 atcaccggcc tgccctcccg catgaggatg ttgtagggcg agaggtcgcc gtggatgagg
      661 tcgagctcga gcatgcggcg cacgcactcg acgagctcct ccttcacgtc ctgggcctcc
      721 ccggggccca ggcgggcgtc gcgcaggcgc ggcgcggcct cctcgccccc cacgaattcc
      781 atggcgatcg aggcgggccc ccgaccgagc ggccgaggca cgggaaggcc cgccgcgtgg
      841 agccgctcca gggcctcgta ctcggcgtcc acccagtagg cctgcatcga gtcggggctc
      901 gagtacatgt ggaggatgtc cctccgcttc ctgatggtcc gccccaccct gccgtccagg
      961 tagtccgaca tgtcgcggaa gccgcgcctc tcgatgtcct tgtagacctt gagtgccacc
     1021 gtggacaggc ccgtggaggg atgggccgtg cagcggtaga cgacggcctc cttcccgctc
     1081 ttcagtatcc cctcgacctc gcctatgatg gcctcgcccg cgaaatgccc ggcgatggcc
     1141 tctatgtccg tttgcatcgt ggacgagggt aggcccgccg gagggaggcc gcaagggaaa
     1201 gctccgctag aaggaagtgt ccgcccgggc ggctgcgcgc gcggcccgct tccaggcgcc
     1261 gccgcccggg cgcgatgttg cggatccggc cgtctcgggt tatgcttctc tttcgcggga
     1321 aaagggagcc atgaagaagc ccaacctgaa gcggtactac cagaacctcg tcaacgcgct
     1381 cagcttctcg tcggagctct acaagctcag gcgcgagctc aacacgaaga tcgacattcc
     1441 cgacgccgtc gtgcccacga cccactcgca cccgctgggc gtgacccgct ggttcaagaa
     1501 gcgccgcatc tccatcgcgg aggcctacct catggtcatc cgcgacctgg agtcgaagca
     1561 gtccaaggcg aggctgcgct ccctgcggat gatggtggag gcctccttcc acacgaagtc
     1621 cctggacctg cccctcaaca cggcccgcgt gcagatggcc ctcatcaagg aggcggtcaa
     1681 gaaccgcggc aaccggaggc gccagctgga gctcctccag gacttctccg tctcctccca
     1741 cggccagtac caggtcatcc gcaagctctg cgacgagctc aacatcatcg agctgccgga
     1801 gacgggcgag ccgctcaagc gcctggacgc gggctgggac gagcacgtcc acgacacggc
     1861 cacctccggc cgcaagaacg ccacccagct cctcatcgac gccttcatca agggcatctc
     1921 cgagctgacg atctcctacg gctccgccgt ctccctcgac atgatggagg aggccgtgga
     1981 ggccggcagg atcgtcggcg tccgcgtgaa gatcggcctc gaattcagca tgatggccca
     2041 ggggaagcgc ttccacttca tggccctcct gccgcccttc aagggcgcga aggacgtcga
     2101 acgctggttc aaggagcacg ccgagggcct caaggagctc ttcgacggcc tggagaagaa
     2161 ccaggagaac cggatcgagg cggtgcggag cctcctcaag aacttcaaca agacctcgct
     2221 caaggagctc aacgagggct accccgacga caggctctat gccgtgccca agctcaagat
     2281 gaagaagctc tccgccttca tccccctgtc gagcatcaac cgcatgcacc tgggcgagtt
     2341 cctctactcc tactacaagc ccgtcctcct gaaccgcatc ctcctcctca aggtgcagag
     2401 ggagaaggcc cgctgcgacc tcaggaagag gctgatctcg gactgggact tcaggatcat
     2461 cgacgagcgc tacacgaggc tccgcgacga gtaccgggag ctcaacccgg acaacctccg
     2521 caagcagtat ttcacgaatc ccaagatcgg cgactaccag acggtcttcg acgacatgga
     2581 ggccatcaag gcctcgctct cccgcgccgg ctgcaggctc aagatcctcc accccctgga
     2641 gtacggcctg gacaaggcga agaagctgct ttcgaagagc cggggcctca tcgacgaggt
     2701 cgagatctac aacatgcagg actccgtcct gcgcgatccc gacgagatcc tgaagctcgc
     2761 gcgctacatc aacgacctga acctggagag cgtccgcgac gggagccccg cctacgtgcc
     2821 ggtctgcggc agcgactcca cgggccggag ccccaagatc ccgggcatgg gattcatcag
     2881 gcaggacagg atcggcggca agtaccgccg gcgctacatc cggcgccatg tcgccctccc
     2941 ctccctcgtc tccgcgatgg tcgaggcgga ggggacgcac gtcacgcagg agatggtggc
     3001 ggccgccccg gtcatcctca gcatgggcaa gatctccggc ggctcggcga acctcatcgg
     3061 cgacgagccc gagaacgagt cgctggagat cggtcccgcg agggcctggc gctacctcaa
     3121 tcccaccctc gtgaacctcg cctacgcggc cgtcggcttc ctcgtcgccg accgcttcat
     3181 cgggcccttc tacgccttcc tctggctctt catcaccggc ttccggaact cgatcgccga
     3241 cctggtcgcg agccgcggca cccgcctggc cgagtggaac tggaagagcg tcaacttcgg
     3301 caacgtcgcg cgctccctct tctggaccgg cttctccgtc cccatcctcg gcttcgtcaa
     3361 ggccaggttc gacctggcct ggccctgggc ccccgagggc cccctcttcg actccgccaa
     3421 gttcttcttc atctccttct cgaacggcct ctacctggcc gcgcacaaca ccctgcgcgg
     3481 cttcgacagg aaggtggtca aggccaactt cttccggagc gtcatagcct ggcccttcgc
     3541 gaccgccttc tcgccgttcg gcagcctcct cggcatcccg agcatcgtgc aggccaagat
     3601 ctggtcggac ttcgtcgcgg gcttcatcga gggcggcggt aagtacatga aggtcctgag
     3661 gctgcgccgc agggacctgg aggacatcgt cccgaggatc atctccgggg accaggagga
     3721 ccgcttcacc gccctcctcg acctcctctt cctctaccgg gaggagccgc gcacggagag
     3781 ctccctcaag gccgtgatgg atcccggcta ccgccccatg ggcatactca aggacgacaa
     3841 ggacgcgccc aagtattcct tcagcgagct ctacgccgcc gtcatggacg agtccatcga
     3901 ccgtcccctc gttgactttg tcctgtcgag gtacggccac gacgtggccg tggacctggc
     3961 cggcctcgtg gccctgaccc tgcctgccat gcgggagtgg ttcgaggccc acgcggcccg
     4021 cttcctcgcg ccgcagcgcc aaccggcggc cgccgcgcgg gaggcggagc ccggggtcca
     4081 gccggcggcc gccgcgcccg tggccgcgcc cgtggccgcg aaaaaggacg cgcctgccgc
     4141 cctgcccgag gccggggcca aggcccggga ctagggctcg ggactagggc tcgggaagct
     4201 cgaactcgag ccgcaaggcc gcgccggggc ccgagcccgg ggccaggcgg ccgccgacct
     4261 gcgacatggc cgcggccgcg atgcgcatcc cgaggctttc ccccgccgcc gggtcgaagc
     4321 cgtcggggag tcctatcccg ctgtccgcga tcgtgatcgc gcaacgcggg ccttcccgcc
     4381 ccccggcgat cgacaccttc cgcggcccct cccagccggg ggggaaggcg tgctcgaggg
     4441 agttggagac gcactcgccg agggccaggg ccaggggcag ggccgtgtcc accggcaggg
     4501 agagctcccc caggtccacg gcgatctcgg cggccgcccg gccgggatgg tcgcggtcgg
     4561 agcgcagggc cgccgccagc tcggaggcga tggcgccgaa gtccacgtgc ctgaaatcct
     4621 gggagcggta gaggctctcg tggaccaggg ccatggagag gacccgcgcg cggaagcgct
     4681 ccagggcggc gagggcccgc gcgtcgtcct cggcccccgc ctggaggccg aggaggctca
     4741 cgatcacctg caggttgttc ttcacccggt ggtggacctc cttgaggagg gcgtcccgct
     4801 cccccaggag cctgcgcagg tccaggttcg cctcggcgag ctcggcggtg cgctcctcca
     4861 cgcggcgctc caggccctcg ttcgcctcgc ggagcccgcg ggagagctcc gccagccgga
     4921 ggtgcacgcc tacccgagcc aggacctcct cgcgcctgaa gggcttcgtg atgtagtcca
     4981 ccccgccggc ctcgaagccc ttgaccttga actcggtctc cgagagggcc gtcatgaaga
     5041 tcaccggcgt ctccgccgtc tcgggatcgg ccttgaggcg cctgcagatc ccgtagccat
     5101 cgccgtcggg aagcacgacg tcgaggagga cgaggtcggg cctcgcgtag cgcgcgcgct
     5161 cgaccccgct cctgccgtcc tcggcgacga ggatggccag gtcgtagccg cccaggtact
     5221 ccgagaggag ggcgaggttc ttcgcgtcgt cgtcgatgat gaggacggcc tgcctcgccg
     5281 ggacgccctt ccctgccccg cccgcggcgg ggatcatgtc cttccctcca tccattcctc
     5341 cgccagggcc agtatgctct tggccctgaa ttccgccgcg agcgccctta ggcgatcggc
     5401 gtggcgcctc gcctcgggcc tatccttggc catgccctcg gcccaggcgg ccactccccg
     5461 catgtcgccg atgcgcgcga gctcgaggag ctcacggagc gccgcctggg agaagccggg
     5521 agggactccg gcctcggcct cgggcggggc ttcggccgcg gctccggcat cggcctcaag
     5581 cgccgcggag gccgaagctc cgcttccccc gccgacgggc gctccgggga aggcctccgg
     5641 gcgcgccgca tgatgcggcg cggcatgggc gggcttcgcc gccgcgcgca gccactcgag
     5701 gccgagtgcc ccgcccatgg ccttgagcag gtcgccgatc cgcaccggct tccgcacgaa
     5761 ggcctcgcag ccggcgccgg cgctcatgag gaggtcctcg gggaaggcgc tcgcgctcac
     5821 ggcgatgacg tggaccggct cggggcctgt ccccgccttg atgcgcctcg cggcctcgat
     5881 cccgcccgtc cccggcatcc gcaggtccat gaggacgagg tcgggccgct ccgccagggc
     5941 tagctccagg gccttggccc cgtcctccgc ctcgaggatg cggaagccga gcggagcgag
     6001 catgtcccgg aggacggagc ggttgtcggg gctgtcgtcg gcgacgagga cggtcctcct
     6061 cggccccgcg tagcccgaga tgccctcctc ccagtccggc gccgcgcctt cggcccggga
     6121 gccgagcggc agctcgagct cgaaccagaa ggagctgccc ctgcccggct cgctccgtat
     6181 ctcgatgtcg ccgcccatcg cgcgcaccag ggcccgcgag atggggaggc ccaggccggc
     6241 cccttccgcg ctcgcggcgg agcttcccgc gcggaagaag ggctcgaaga cccgtgcgag
     6301 gtcctcggag gcgaggccga tccccgtgtc ggccacctcg aagcggaggc gggcctttcc
     6361 gcccttgccg tcgccggggg gcctcgcgtc gcccgggccg gccgagccgg tccctccccg
     6421 cggcccggcg gtggacacgg agaagcggac ctcgccgctt tgcgtgtact tcaccgcgtt
     6481 cccgaggaga ttgaggagga cctgccggag acgcgtctcg tcggcctcga ccgccgcggg
     6541 cagaccctcg cccggaaggt aggcgaagcc gatgcccttg gcctccgccc gcgtcgcggc
     6601 gatgccggcc acgccggaga ggaaggcccc caagtccagt gcggtcggat ggagggtcat
     6661 ccgcttggcc tcgatccgcg aaatgtcgag gatgtcgttg atcagggtga ggagatgctc
     6721 gccgctccgc tcgatcgtgc cgaccgcgaa atcctgcgag gcggagaggc cgccgttccg
     6781 cttgaggatc tgcgcgtagc cgatgatgcc gttgaggggc gtccggagct cgtggctcat
     6841 gttcgcgagg aattcgctct tggcccggct cgcggactcg gcggtctcct tggcggcgac
     6901 gagctcggcg gtccgctcgg cgaccaggtc ctccaggtgc tcgcgccccc gcttgagctc
     6961 ctcctccgcc ttcttgaggg cgtcgatgtc ggcgatgaag ccgtcgatga aggccatgcc
     7021 gccggaggcg tccggcacgg cgcgggcgct caaggagacc cagatggcgc tcccgtcctt
     7081 gcgccggagg aggacttcct tgccgatcgc ggcgccccgg gccttcaagt cggccaggaa
     7141 cccgtcccgg tccgagccgc ggacgtagag ctggctcctc acgtcgccca ggcccgcgac
     7201 gagctcctcg ggggaatcgt agcccaggat gcgggccagc gaggggttcg cgcggagaag
     7261 gcggccggag acggaggtgc ggaagatgcc ctccagcgcg ttctcgtaga tgccgcggta
     7321 cttgttctcg ctctcccgga ggccctcgag ggtcgcgcct atcctctcgc tcatgaggtt
     7381 gaaggtgcgc gccagcgtgc cgagctcgtc ccgcgagccg acctcgacgc gctcggccga
     7441 gcctcccgcc atgaggacct ccgcggcccg cgtgaccttg cggacgggcc ccgtgatgct
     7501 cctggccagg gctatgcccg cgaggccgac gacgagggtc atggccaggc cagagaccgc
     7561 catcgtggcg gccatcccgg cgctgccccg ctggagcacg gcgcggtagc cctcgagctc
     7621 gtcctcgtag acgctcgcgc ctatcaccca gtcccagggc tcgtagtacg cgaggcgcgc
     7681 caccttccag cgcggagccg cctcgccggg attgcgccat tcgtagcgga tggtcgtctt
     7741 ctcgccgggg ccgagggcca tgccctgctc cacgatctcg tgcgtgacgt agcggccgtc
     7801 ggagtcccgc acgtcccaga cgtactcgcc gtcgcgcgtt ccgccctgcg agatcacgta
     7861 gcgccccctg tcctcgccgg agcccgtgat gacgaagacg tagcccgtct tgcccaggcg
     7921 gaggtccagg atagcctcgc ggatcctggc ctcggcgctc ttctggatcg tcccgacgta
     7981 gagcatgccg atcgtccgtc ccgaggcgtc ctggatcggc tcgtaggccg tgaggtacca
     8041 gtcgttcacg acgtaggctc ggccgaggta ggtccggccc gccagcgcgg ccgccaccac
     8101 ggcgttcggc atcccgtcgg gctcgcgggc ggggatgtag gtgcccaccg cgcgcttccc
     8161 ctcagagtcg aggacggtcg tcgcgacgcg gagcatgtcg ccatcctcgc ccgcccgctg
     8221 gaagaccgtg gccgagccgc cgaccaggga gactatctcg tcgacgaggg gcacgggcct
     8281 ccccggttcg gtctcgtcgg caaggctcgt ccgcccagga ggacgcgggg gaggacgaac
     8341 tcctccacgc tccccgaaaa ctggttaatg gcggaccagg agaccttttc cctcgactcc
     8401 gagatcccgc ccgagcgccc cagcatcctt ctggccacgt tgaggctgga ggccacggcc
     8461 tggcggacgg cctcgtcctc cgcgcgcacc aggttgtagg cgctcgtcgt cacgtggccc
     8521 aggtcctcgt ccacgaggct gtccacctcg cgctgggcca gggcgttgta gtccgagctc
     8581 aaggccgcgg cgaggaggac gagggccagg gcggccacga aggcgctgcc cgccgcgagg
     8641 agggagatct tggcgtcgag cctgcgaagc cactggaaac cgcccaccgg cgtagcctcc
     8701 ccgcactccg agccctgcgc gccctcgaag ccgccgagcc cggacgcctt gcgccccccg
     8761 taggtccccg gagaggcggc catggcgcga aaaccttacc tacaccatag gccaagggag
     8821 gctcggcctc aaggcgcggc gatgggattc acggcgcggc gatgggtttc acggcgcggc
     8881 gatggtagtc gcggcgcggc gatggtagtc acggcgcggc gatggtagtc acggcgcgca
     8941 gggagtcgtc ctggccggcg gatcggggct cgggctaggg cttcaggacg gcatcgaagt
     9001 cggcgcggta cttccggaag agggcgaggt cggcgggatc ggctgtctcc atctccccta
     9061 gcctgcccag catggccccg agctccgcgt cgacggcggc gaggagggag gccttcgtcg
     9121 cctcgtccag gtcggggtcg gcgagtatct cttccctggc cgcggccatg gcctcattgg
     9181 cgacggcgcc gcccatctcg gccgctccca tgccgcccga gagctccggg agcatgtccg
     9241 cgcagtagac gtcggcgaag cgcttgacct gttcggcctc gaggacggtc atctccctgc
     9301 ccgtattgaa gcgcagggtc gccgcctcga gccgcttctc gggcggcagc gtcgcggccg
     9361 cctcgcggca gtagcgcagg agggtgccga ggaaggcgtc ggtcaggagg ggctccgcgc
     9421 cgagcgcggc gggcaggacc agaagcgcga cgagggcgag ggaaaggaaa gtcgccgtgt
     9481 gctttttcat gagggcctcg atatcgttgg atgatacgag gagtatatac cctccgcaga
     9541 cccggagaat aaaaaagcgc ccctttcggg gcgccttgcc taaagtccgg ccttggcggc
     9601 tagacgcgct tgtagtcctt gatggccttc ttgagcgtcg actcgagctc gtcccggtgg
     9661 atgcggacct gctccatgga gtcgcggaag cggaccgtga cggtgccgtc ctccttggtc
     9721 tggtagtcga cggtgacgca gaaaggcgtg ccgatctcgt cctggcggcg gtagcggcgg
     9781 ccgatcgcgc ccgactggtc gtagtccgtc gcgaactcct ccttgaggcc cgtgcggatc
     9841 tcctgggcga gctcggcgag gccgtccttc ttcatgaggg ggagtacggc gacggtgacc
     9901 ggcgcgaggt tcgggtggaa gcgcatgacg gtgcgccaat cgtcgtcgtt gcccttgtcg
     9961 gcgaccttct gctcctcgta ggcgtcgcag aggatcatga ggagctggcg ggtgaggccc
    10021 gcggaggtct cgatgatgta ggggatgtag cgctcgttgc cattgtcctg gtcgatgtac
    10081 tggaggtcct tgccggagaa ctcctggtgg cgcttcaggt cgaagtcggt ccggttgtgg
    10141 acgccctcga gctcct
//
LOCUS       DAOOSJ010000075         9975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4398_length_9975_cov_6.268044, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000075 DAOOSJ010000000
VERSION     DAOOSJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_9975_cov_6.268044"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..981)
                     /locus_tag="PLG14_05255"
     CDS             complement(103..981)
                     /locus_tag="PLG14_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOX48576.1"
                     /translation="MSHCIAMADDPRCASRDLRSGPAARTAILLGAALLFAVPAAFAG
                     EGWEFSLGAGGFASSAYPGSDERFLAPVPMAKAAYSSGGFEASASVMEGLGLRYVDME
                     ARVFASLGLNFGEKRGAEEFSAGLFSRRHDAETRSLLEGSPETESLLRAGLKLGWISP
                     VGVVGASLDYHPTRVEGAGLEHGLLGSAFWLAPVPLSDRLTVTAMLALEAMDGAYAEA
                     WHSVGAPTAELEAFEARAGLRSCLVFLGLDYAFAERWTASLVAANGFLLADAARSPYT
                     RSRYQPTFALTTAYAF"
     gene            complement(1197..1727)
                     /locus_tag="PLG14_05260"
     CDS             complement(1197..1727)
                     /locus_tag="PLG14_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOX48577.1"
                     /translation="MISKKMSDRINLQINREMFSAYLYMAMSARMAEAGYKGIANWLM
                     IQYHEEMFHAMKLYGYLEDQGAAVALEAIAKPEFKETGVKELFRHVLEHEKGVTASIR
                     EIAALAREEKDYATESFIAWYVGEQVEEEKNAADILQTLDLLGDSAQGTFMLNIELGK
                     RKLGVPSDFTAMGGGD"
     gene            complement(1810..2715)
                     /gene="thrB"
                     /locus_tag="PLG14_05265"
     CDS             complement(1810..2715)
                     /gene="thrB"
                     /locus_tag="PLG14_05265"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOX48578.1"
                     /translation="MSAVRVRVPATTANLGPGFDALGIALRLYDRFEAEEAEGLAVEG
                     CEPAYADEDNLFVRAYRLGLEELGLPFRGLRVRFRADIPIARGLGSSSACVVGGLLAA
                     SAAGGRGDPRWGALGRERILDLAARLEGHPDNAAPAVLGGFAVSVLEEGRATALRFPL
                     GEELAFHALVPPFELSTAKARAALPPSVPFKDAVRNSGRAALVAAALAARDYGALGLA
                     CSDRLHEPYRTPLVPGFAEVTAAAREAGALAVFLSGAGPTVMALALAEDSGFAARMAP
                     ALAARPEGAWRLLRLLPDDEGAVLD"
     gene            complement(2712..4424)
                     /gene="thrC"
                     /locus_tag="PLG14_05270"
     CDS             complement(2712..4424)
                     /gene="thrC"
                     /locus_tag="PLG14_05270"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOX48579.1"
                     /translation="MAGGALRYRSTRSGAAPVSSARALLEGLAPDGGLYLPERLPPLD
                     PRVLSEEPLSYPELAYLVLRPWFPDLPEEGLRAALRASASRFETEEVAPLVSRGGFHF
                     LELFRGPTLAFKDLALTLLGDLFALAAVAEAGAGGRSEDAVPPAGLRTAASAAAGGPS
                     GAPGGEDILVLVATSGDTGKAALEGLAGRPGVRVAVFYPSEGVSPVQRLQMTSHDAAN
                     AYVAAIRGNFDDAQAGVKALFAEAADPSSPFARGLAARRLRLSSANSINVGRLAPQLV
                     YWVKAWRDLRARGLLGPSGGMDVAVPTGNFGDILAAWYAKRAGLPIGTLLSASNRNRV
                     LADFFRTGVYDRARPFYRTESPSMDILVSSNLERLLFHATGEDPARTAALAASLASRG
                     RFELQGAERDRIGDFRGAWADEAEAAAAARRLHEASGYVVDPHTAVALAAASSLAGAD
                     PVVVAATASPFKFPVTTARALGLDLGAAGPDGAAAAPAGGAMASGAAGGAADAKVAGA
                     AGGSCADPAAELDAAEALSRAAGLPLPAALRDLRGKPAVHGDLLEPGDMRAAVLAFAT
                     KELP"
     gene            complement(4426..5361)
                     /locus_tag="PLG14_05275"
     CDS             complement(4426..5361)
                     /locus_tag="PLG14_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX48580.1"
                     /translation="MELALDSIRSRYSVRTYDRSPLSGAEEEALRAAFQEALPAPFGT
                     SPRFALVAAEAAGQAAKMGTYGLVSGAPAYVVGAAPLVPGGMEDFGYAMEGIVLRAAE
                     LGLGTCWLGGVYDRGRAARALALREGEAVVAALAVGRPADRRSLPDRIVYGLSGARGR
                     KPPSELFFAPPPAPGAAAAPSAEVGQAAAARPDGEGWEPLPQGSPWLAEPRSELLEAL
                     RLGPSASNKQPWRLVLEGGAEPALHLYLHEDQKYNNALGPVKIQLVDMGIAMRHVEAA
                     ALALGVPGSWRRLPAAPFAATPPRSYVASFLSGRA"
     gene            complement(5371..5940)
                     /locus_tag="PLG14_05280"
     CDS             complement(5371..5940)
                     /locus_tag="PLG14_05280"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOX48581.1"
                     /translation="MERCPWCGSDPLYVAYHDAEWGCPVHDEGRHFEFLLLETQQAGL
                     SWLTILRKREAYRRAFAGFDPEKVARFGEADVERLLSDASIVRNRRKLEGAVRNARAF
                     LAVAEEKGSFDAYLWDFVDGKPLNPGRRSLAEIPATTPLSDRVAADLKARGFAFVGST
                     TVYAHLQAVGLVNDHLASCFRWAELGGQA"
     gene            complement(5958..>6273)
                     /locus_tag="PLG14_05285"
     CDS             complement(5958..>6273)
                     /locus_tag="PLG14_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOX48582.1"
                     /translation="MGEFGAYLRPCAENDIAVQRALVETEEAELALALVGLDEASRNI
                     FLRNMSERGKASLLSALARAEERLGGRGPAGAGGPSSEVAAARAALAARLEAASRLPP
                     PR"
     assembly_gap    6274..6373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6374..7156
                     /locus_tag="PLG14_05290"
     CDS             <6374..7156
                     /locus_tag="PLG14_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48583.1"
                     /translation="ALGFLGVVAVNGLANALPLNGVGTGELSDELPNLFVPAGLTFAV
                     WGLIYLLLSGYVAFVLAEAFGKRRGAAWERGDGWLFAANAAANIAWIFAWHWRDVTVA
                     LALMAVILYTLIALEERLLPRLEEGGSLAAAPGDRKAQLRRFFLSVPIRVYLGWILVA
                     TIANFTAFLVKGGWDGFGLDQRIWAVAVIGAALALALYYALGRGAIATPLVVVWALAG
                     IVIKRTSVDPAYSAPVWTAAAAAGLAILAAIAVERLRSYRRP"
     gene            7221..8243
                     /locus_tag="PLG14_05295"
     CDS             7221..8243
                     /locus_tag="PLG14_05295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48584.1"
                     /translation="MSDSRPDALPVGPADLPDFLAYAAAYGPSHDESFVPGPGFAPGP
                     EKPSFLLRGPSGEAAGAVSLLLSRSQLASGRGRFALLHSGGAGGGSREAYRALLRAAL
                     GAAGGKVRSLYLFLPEELAQPRGWLEEAGFSYERTAYLMGREPDGPGGATGGAAGMGG
                     LGGVTGGPGGATAARMGPPGAALPEGYSLEAVERGDKARLAEFVAVRNRNFREVAGSS
                     PATVEDWEEHFDEPEALPGGILLLRSPEGEACGTLFLERDEEEGSVFIGAVSVDEGRR
                     GRGLGRALVREAVSRGAALGLPRAFLSVNARNDKALGLYLAEGFTARKAMCCLSAGLE
                     ELARRI"
     gene            8367..9698
                     /locus_tag="PLG14_05300"
     CDS             8367..9698
                     /locus_tag="PLG14_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOX48585.1"
                     /translation="MASELLLLFALILVNGFFSLSEMAIVSSRKARLRHEAERGRKAY
                     KLALETAERPSRFLSTIQVAITLVGTLTGAIGGATIAKQLEAAFLRMELLAPIAATLS
                     LGIVVVLTTFVSVVFGELVPKSLALAKPESVAAAVIRPVNILSLVFAPLARLLSAATE
                     LVVRLLGAGKNPEPAVTEDEVMVLIAQGAESGVFDDREREMVEGVLSLGDRRVTSLMT
                     PRPEIVYVDLEDGTEAVRRGVLSNARYGYLPAVEGDLDHVVGMLPVKEYLAAAAEDSE
                     VDPRACMRKPVLVPESISALKAFSAIKEGEVKTALILDEYGGVSGLVALADLMASVIG
                     DLPSTGDGEEPGVTLREDGSWLVDGSLPIDRFVAELGLEEELLDGAYETVAGLVLDRM
                     GSIPKAGETCRWDGCRIEVVDMDGNRIDKVLVAKLPEDEDEAGTPPEPIWT"
     gene            9782..9970
                     /locus_tag="PLG14_05305"
     CDS             9782..9970
                     /locus_tag="PLG14_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48586.1"
                     /translation="MSQELYPVGSKRCFACIQWEGRRSYDPEKKLIKADAGSAGRCLV
                     IHQDVKGSHYCDNYFPLR"
BASE COUNT         1369 a         3503 c         3675 g         1328 t
ORIGIN      
        1 ggaatcctag gcgtccggac gcgggcttcg gtcggggctg accgccgcgc gggcttcgcg
       61 gccggaccgc ccggccctgc cgcgagcgcc gcaaggccgc tcctagaagg cgtaggccgt
      121 cgtgagcgcg aaggtaggct ggtaccgcga gcgggtgtag ggactcctcg cggcgtccgc
      181 gaggagaaag ccgttcgccg cgacgaggct cgccgtccag cgctccgcga aggcgtagtc
      241 gaggcccagg aagacgaggc agctccgcag gccggcgcgg gcctcgaagg cctcgagctc
      301 cgcggtgggt gcgccgacag aatgccaggc ttccgcgtag gccccgtcca tcgcctccag
      361 ggcgagcatc gccgtcacgg tgagccggtc ggagaggggc accggggcca gccagaaggc
      421 cgagcccaag aggccgtgct ccaacccggc gccttccacc ctggtcgggt ggtagtcgag
      481 ggaggcgccg acgacgccga cggggctgat ccagccgagc ttcagccccg cgcggaggag
      541 gctctcggtt tccgggctgc cttcaaggag gctccgggtc tccgcgtcgt gcctgcggga
      601 gaacaggccg gcgctgaatt cctcggcgcc gcgcttttcc ccgaagttga ggcccaggct
      661 cgcgaagacg cgagcctcca tgtcgacgta cctcaggccg agcccctcca tcaccgaggc
      721 ggaggcctcg aagcctcccg acgaataggc ggccttggcc atcgggacgg gcgccaggaa
      781 tctctcgtcc gagccggggt aggccgagga ggcgaagccg cccgcgccca ggctgaattc
      841 ccagccctcg ccggcgaagg cggcgggcac ggcgaagagg agggcggctc ccagaaggat
      901 agcggtgcgg gcagcgggcc cggaccggag atcgcgcgag gcgcaccgcg gatcgtcagc
      961 catggcgata caatgactca ttcttgtttc ctttcgttct cggtcggcca cgcaaggccg
     1021 gccagtgtgc agggggatac tcctggccta tcgacactac cccatagacc gactgtcggt
     1081 ctatggggcg agaaactctt ttactggccg tggttccgcg caaaacccac aaaaaaaccc
     1141 cgggctccat gcccggggct tcgatactgt gtaggccgct tagcggctgt actccttcag
     1201 tctccgcccc ccatcgccgt gaagtccgag ggcacgccga gcttgcgctt cccgagctcg
     1261 atgttgagca tgaaggtgcc ctgggcggaa tcacccagga ggtcgagcgt ctggaggatg
     1321 tcggcggcgt tcttctcctc ctctacctgc tcgcccacgt accaggcgat gaaggactcg
     1381 gtggcgtagt ccttctcctc gcgggccagg gccgcgatct cgcggatgct cgcggtcacg
     1441 cccttctcgt gctcaaggac gtggcggaag agctccttca ctccggtctc cttgaactcc
     1501 ggcttggcga tcgcctcgag ggcgacggcg gcgccctggt cctcgagata gccgtagagc
     1561 ttcatcgcgt ggaacatctc ctcgtggtac tggatcatga gccagttagc gatgcccttg
     1621 tagccggcct cggccatccg ggcggacatg gccatgtaga ggtaggctga gaacatctcg
     1681 cggttgatct ggaggttgat ccggtcgctc attttcttgg atatcatggt ggctcctttg
     1741 tcggttttgt tagacgtatc taacttaatg catgcggagg gaaaaagcaa attcgaggag
     1801 gcggagcgcc tagtcgagga cggcgccctc gtcgtcggga aggaggcgaa ggagacgcca
     1861 ggcgccctcg ggcctcgcgg ccagggccgg ggccatccgg gcggcgaagc ccgagtcctc
     1921 cgcgagggcc agggccatca cggtagggcc ggccccggag aggaagacgg ccagggctcc
     1981 ggcctcgcgg gcggcggcgg tcacctcggc gaagcccggg acgaggggtg tgcggtaggg
     2041 ctcgtggagg cggtccgagc aggccaggcc cagggcgccg tagtcccgcg ccgcgagggc
     2101 ggcggcgacc agggcggccc ggcccgagtt ccgcaccgcg tccttgaagg gcacggaggg
     2161 cggcagggcg gcgcgggcct tcgccgtgga gagctcgaag ggcggcacca gggcgtggaa
     2221 ggcgagctcc tcccccaggg ggaagcgcag ggccgtggct cggccctcct ccagcacgga
     2281 gaccgcgaag ccgcccagga cggccggggc cgcgttgtcg gggtggccct ccaggcgggc
     2341 ggccaggtcg aggatcctct cgcggcccag ggcgccccag cgcgggtcgc cgcgtccgcc
     2401 cgccgcgctc gcggccagga ggccgcccac gacacaggcg gagctcgagc ccaggccccg
     2461 cgcgatgggg atgtcggcgc ggaagcggac gcggaggccg cggaagggaa ggccgagctc
     2521 ctcgaggccc aggcggtagg cgcggacgaa gaggttgtct tcgtccgcgt aggcgggctc
     2581 gcagccctcg acggcgaggc cctcggcctc ctcggcctcg aagcggtcgt agagtcgcag
     2641 ggctatcccc agggcgtcga agccggggcc gaggttggcc gtggtcgcgg gtacccggac
     2701 gcggacggcg ctcatggcag ttccttggtg gcgaaggcca ggaccgcggc ccgcatgtcg
     2761 ccgggctcca ggaggtcgcc gtggacggcg ggctttccgc gcaggtccct gagggcggcc
     2821 ggcaggggga gaccggcggc gcgggagagg gcctccgccg cgtcgagttc ggccgcggga
     2881 tcggcgcacg agccgcccgc ggcgccggcg accttggcgt ccgccgcgcc gcccgccgcg
     2941 ccactcgcca tcgcgccgcc tgccggcgcc gcggccgcgc cgtccgggcc ggccgcgccg
     3001 aggtccaggc cgagggcgcg ggccgtcgtg acggggaact tgaaggggct cgccgtcgcc
     3061 gcgacgacga cgggatccgc gcccgcgagg gaggaagccg ccgcgagggc gacggccgta
     3121 tggggatcga cgacgtagcc cgaggcctcg tggagccggc gggcggcggc ggccgcctcg
     3181 gcctcgtcgg cccaggcgcc gcggaaatcc ccgatcctgt cccgctcggc cccttggagc
     3241 tcgaagcggc cgcgcgaggc gagggaggcg gcgagggccg ccgtcctggc cggatcctcg
     3301 cccgtcgcgt ggaagaggag ccgctccagg ttgctcgaca ccaggatgtc catggagggg
     3361 ctctccgtcc tgtagaaggg ccgcgcccgg tcgtagacgc cggtccggaa gaagtccgcg
     3421 aggacgcggt tccggttgga ggcggagagg agggtcccga tcgggaggcc ggcgcgcttc
     3481 gcgtaccagg cggcgaggat gtcgccgaag ttgcccgtcg ggacggccac gtccatcccg
     3541 cccgaggggc cgaggaggcc gcgggcgcgg aggtcgcgcc aagccttcac ccagtagacg
     3601 agctggggcg cgaggcggcc cacgttgatc gaattggcgg aggagaggcg gaggcgccgc
     3661 gcggcgagcc cgcgcgcgaa ggggctcgag gggtcggccg cctcggcgaa gagggccttc
     3721 acgcccgcct gggcgtcgtc gaagttgccg cggatcgcgg cgacgtaggc gttggccgca
     3781 tcgtggctcg tcatctggag gcgctggacc gggctcacgc cctccgaggg gtagaagacg
     3841 gcgacgcgga cgcccggcct cccggccagg ccctccaggg cggccttgcc cgtgtcgccc
     3901 gaggtcgcga ccaggacgag gatgtcctcg ccgccgggcg cgccggatgg cccgccggcc
     3961 gcggcagagg cggccgttcg caggccggcc ggcgggaccg cgtcctccga gcggccgccc
     4021 gcgcccgcct cggccacggc ggccagggcg aagaggtcgc cgaggagggt gagggccagg
     4081 tccttgaagg cgagggtcgg ccccctgaag agctccagga agtggaagcc gccccgcgag
     4141 acgagggggg ccacctcctc ggtctcgaag cgcgaggccg aggcgcgaag ggcggcgcgg
     4201 aggccctcct cgggcaggtc ggggaaccag ggcctcaaga cgagataggc gagctcgggg
     4261 taggagagcg gctcctccga gaggacgcgg ggatcgaggg gcggcaaccg ctcgggcagg
     4321 tagaggccgc cgtcgggcgc gaggccctcg aggagggccc tggccgaaga gacgggggcc
     4381 gcgccggatc tcgtgctccg gtagcggagg gcgccgccgg ccatgctagg cccggcccga
     4441 gaggaagctc gcgacgtagc tcctgggcgg cgtggcggcg aagggcgccg cggggaggcg
     4501 gcgccaagag cccgggacgc ccagggccag ggccgcggcc tcgacgtggc gcatcgcgat
     4561 gcccatgtcg acgagctgta tcttcacggg cccgagcgcg ttgttgtact tctggtcctc
     4621 gtggaggtag aggtggaggg ccggctcggc cccgccttcc aggacaaggc gccagggctg
     4681 cttgttggag gccgaagggc cgaggcggag ggcctcgagg agctcggacc tcggctcggc
     4741 gagccagggc gagccttggg gcaggggctc ccagccctcg ccgtccggcc tggcggcggc
     4801 cgcctggccg acctcggcgg aaggcgcggc ggcggcgccc ggcgcggggg gcggggcgaa
     4861 gaagagctcg gagggcggct tccggccgcg ggcgccggag aggccgtaga cgatgcggtc
     4921 gggaaggctc cgccgatcgg cgggacggcc cacggccagg gcggcgacca cggcctcgcc
     4981 ctcgcggagg gcgagagccc gggccgcgcg gccccggtcg tagaccccgc ccagccagca
     5041 ggtcccgagg ccgagctcgg cggcgcggag gacgatgccc tccatcgcgt agccgaagtc
     5101 ctccatgccg ccggggacga ggggggcggc gcccaccaca taggccggcg cgccggagac
     5161 taggccgtag gtccccatct tcgccgcctg cccggccgcc tccgccgcga ccagggcgaa
     5221 gcgcgggctc gttccgaagg gggcgggaag tgcctcctgg aaggccgccc tcagcgcctc
     5281 ctcctcggcc ccggaaaggg ggctcctgtc gtaggtccgg acggagtagc gcgatcggat
     5341 cgagtcgagg gcgagttcca tggcggttcc tcaggcctgc ccgccgagct cggcccagcg
     5401 gaagcaggat gcgagatggt cgttgacgag gccgacggcc tggagatggg cgtagacggt
     5461 ggtcgagccg acgaaggcga agccgcgggc cttgaggtcg gcggcgaccc ggtcggagag
     5521 gggcgtggtc gcggggatct ccgcgaggct ccggcggccc gggttcagcg gcttcccgtc
     5581 cacgaagtcc cagaggtagg cgtcgaagga gcccttttcc tccgcgaccg cgaggaaggc
     5641 gcgggcgttc cggaccgcgc cctcgagctt gcggcggttg cgcacgatgg aggcgtcgga
     5701 gaggagccgc tccacgtcgg cctcgccgaa gcgggcgacc ttctccggat cgaagccggc
     5761 gaaggccctc ctgtaggcct cgcgcttacg caggatcgtg agccaggaga ggccggcctg
     5821 ctgggtctcg aggaggagga actcgaagtg cctgccctcg tcgtggacgg gacagcccca
     5881 ctcggcgtca tggtaggcga catagagcgg gtccgagccg caccagggac agcgttccat
     5941 gccggaaaga tagcctccta gcgcggagga ggcaagcgcg aggcggcctc aagccgggca
     6001 gcgagggccg cccgcgcggc ggcgacctcg gacgatggtc cgccggctcc ggccggtccg
     6061 cgcccgccca ggcgctcctc cgcccgcgcc agggcggaga ggagggaggc cttgccccgc
     6121 tcgctcatgt tccggaggaa gatgttccgc gaggcctcgt ccaggccgac gagggccagg
     6181 gcgagctccg cctcctccgt ctcgacaagg gcccgctgca cggcgatgtc gttctccgcg
     6241 cagggccgca ggtacgcgcc gaattcgccc atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnngccctgg gcttcctggg cgtcgtcgcg gtgaacggcc tcgcgaacgc
     6421 cctcccgctg aacggcgtgg gcaccggcga gctctccgac gagcttccga acctcttcgt
     6481 gccggcggga ctcacctttg ctgtctgggg actcatctac ctcctcctct caggctacgt
     6541 cgccttcgtc ctcgccgagg ccttcgggaa gcgccggggc gcggcctggg agcgcggcga
     6601 cggctggctc ttcgccgcga acgcggccgc caacatcgcc tggatcttcg cctggcattg
     6661 gcgcgacgtc acggtcgccc tcgccctcat ggccgtcatc ctctacaccc tcatcgccct
     6721 ggaggagagg ctcctgcccc gcctcgagga gggcggaagc ctggccgccg cgcccggaga
     6781 ccggaaggcc cagctccggc gcttcttcct ctccgtcccc atccgcgtct acctgggctg
     6841 gatcctggtg gcgacgatag cgaacttcac cgccttcctc gtgaagggcg gctgggacgg
     6901 cttcggcctg gaccagcgga tctgggcggt ggccgtcatc ggcgcggccc tggccctggc
     6961 cctctactac gccctggggc ggggcgccat cgcgacgccc ctggtcgtgg tctgggccct
     7021 cgcgggcatc gtcatcaaga ggacgagcgt cgacccggcc tactccgcgc ccgtctggac
     7081 ggccgcggcc gccgccggcc tggccatcct cgccgccatc gcggtggagc gcctgcgctc
     7141 gtatcggagg ccctaggagc gagcggctat agccgttccg tcccttccgg cgacccgcac
     7201 gcgggcgtta tcctgcggcc atgagcgact cgagacccga tgccctgccg gtgggcccgg
     7261 ccgacctacc cgacttcctc gcctacgccg cagcctacgg cccctcgcac gacgagtcct
     7321 tcgtgcccgg gcccggcttc gcgcccggcc ccgagaagcc gagcttcctc ctccgcgggc
     7381 cttcaggcga ggccgcgggc gccgtctccc tcctcctcag ccgctcccag ctcgcctcgg
     7441 gccgcggccg cttcgccctc ctccactcag gcggagccgg aggcggcagc cgcgaggcct
     7501 accgcgccct cctccgcgcc gccctcgggg ccgcgggcgg caaggtccgg tccctctacc
     7561 tcttcctccc cgaggagctc gcccagccgc ggggctggct cgaggaggcc ggcttctcct
     7621 atgaaaggac ggcctacctc atgggccgcg agcccgatgg cccaggcggc gcgacggggg
     7681 gcgcggcggg catgggcggc ctaggcggcg tgacaggcgg cccaggcggc gcaacggcgg
     7741 cgcggatggg cccgcccggc gcggccctgc ccgagggcta ctccctggaa gccgtcgagc
     7801 ggggggacaa ggcccgcctc gccgagttcg tcgccgtccg caaccgcaac ttccgcgagg
     7861 tcgcgggctc ttcgcccgcg acggtcgagg actgggagga gcacttcgac gagcccgagg
     7921 cccttccggg cggcatcctc ctcctccgct cgccggaggg cgaggcctgc ggcaccctct
     7981 tcctggaacg ggacgaggag gagggctccg tcttcatcgg cgcggtctcc gtcgacgagg
     8041 ggaggcgcgg gcggggcctg ggccgagccc tcgtcaggga agcagtctcc cggggagccg
     8101 ccctcggcct gccccgggcc ttcctctccg tcaacgcgcg gaacgacaag gccctgggcc
     8161 tctacctcgc ggagggcttc acggccagga aggcgatgtg ctgcctctcc gccggcctcg
     8221 aggagctcgc gaggcggatt tgacgaaggg ggcgggcgca tgttattcct tatgcatcat
     8281 ggatagcgat acagcctccg aacccgcccc ccaaccaccg tctagcgagc ctctagggcc
     8341 gcgccgcctt gcgaagggag gccggcgtgg cctctgagct cctcctcctc ttcgcgctga
     8401 tcctggtcaa cggcttcttc tccctctccg agatggcgat cgtgtcgtcc cggaaggccc
     8461 gcctgcgcca cgaggccgag cgcggccgca aggcctacaa gctcgccctg gagaccgcgg
     8521 agcgcccctc ccgcttcctc tcgacgatcc aggtggcgat caccctggtc ggcaccctga
     8581 cgggcgccat cggcggcgcg acgatagcca agcagctcga ggccgccttc ctgcgcatgg
     8641 agctcctggc cccgatcgcg gccacgctct ccctcggcat cgtcgtcgtc ctcaccacct
     8701 tcgtctccgt cgtcttcggc gagctcgtcc cgaagagcct ggccctcgcc aagcccgagt
     8761 cggtcgccgc ggccgtcatc aggcccgtca acatcctgtc cctcgtcttc gcccccctgg
     8821 cccgcctgct ctccgcggcg acggagctcg tcgtgcggct cctgggcgcc ggcaagaacc
     8881 cggagccggc cgtcacggag gacgaggtca tggtcctcat cgcccagggc gccgagtcgg
     8941 gcgtcttcga cgaccgcgag cgcgagatgg tggagggcgt cctctccctg ggcgaccgcc
     9001 gcgtcacctc cctcatgacc ccccgccccg agatcgtcta cgtggacctg gaggacggca
     9061 ccgaggccgt gcgccgcggc gtcctttcga acgcgcgcta cggctacctc cccgccgtgg
     9121 agggcgacct ggaccacgtc gtcggcatgc tgcccgttaa ggagtacctc gcggccgcgg
     9181 ccgaggactc cgaggtcgat cctcgcgcct gcatgcgcaa gcccgtcctc gtgcccgagt
     9241 ccatctccgc cctcaaggcc ttctccgcga tcaaggaggg cgaggtgaag accgccctca
     9301 tcctggacga gtacggcggc gtctcgggcc tcgtcgccct ggccgacctc atggcctccg
     9361 tgatcggcga cctgccgagc accggcgacg gcgaggagcc gggcgtcacc ctgcgcgagg
     9421 acggcagctg gctcgtcgac ggctccctcc ccatcgaccg cttcgtcgcc gagctcggcc
     9481 tggaggagga gctcctggac ggcgcctacg agaccgtcgc cggcctggtc ctggaccgca
     9541 tgggctcgat ccccaaggcc ggcgagacct gccgctggga cggctgccgg atagaggtcg
     9601 tcgacatgga cggcaaccgc atcgacaagg tgctcgtggc caagctgccc gaggacgagg
     9661 acgaggcggg gacgccgccg gagcccatct ggacctgaaa gccgatctcc agccgagggc
     9721 ggaatcggcc gatattcgag atgcccctcg cgggggcgca tcacgaaaat ggaggcccgc
     9781 gatgtcacag gaactctatc cggtcggctc gaagcgctgc ttcgcctgca tccagtggga
     9841 agggcgccgg agctacgacc ccgaaaagaa gctcatcaag gcggacgcgg gctccgcggg
     9901 ccgctgcctg gtcatccacc aggacgtgaa gggctcgcac tactgcgaca actacttccc
     9961 gctccgctag gactt
//
LOCUS       DAOOSJ010000076         9892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4439_length_9892_cov_4.569991, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000076 DAOOSJ010000000
VERSION     DAOOSJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_9892_cov_4.569991"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="PLG14_05310"
     CDS             complement(<1..2820)
                     /locus_tag="PLG14_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX48587.1"
                     /translation="MSDEEKVLVLRLAGLEGEGGLEAKPGDRLMEFHVSRRARDELGL
                     AGSLFRSTGNVVLPDFAAARLLARRINEVVDAALLPERAVRAGRLNAMALIDEILHDV
                     ARLYRERASAEAFPALLSALEKELGKEALDGLLAAFVRDFPPLAVYRREATEKAWLAA
                     SRDGVPNRILALEELLLLRLANENPAFGPYRFLFDDGSRPSGEAPLPASLAALPAYAA
                     AMAAAERAFSALPRFGPDDQDLLTLLRAPVRAAPYSLPGQLDYIRRRWGLLVGDKLLR
                     LLGGLDLIREEEKPRFPGPGPTRAYAYRGMEKEYERFTPDKDWMPNVVMMAKSVLVWL
                     HQLSVAYGREVARLDQIPDEELDLLATRGFNGLWLIGLWERSQASAEIKRRCGNPEAA
                     ASAYSLYDYEIAGELGGWPALESLKGRCALRGIRLAADMVPNHVGIDSAWVRERPELL
                     MRAEACPFPGYSFESGNLSGDGRVDVRIEDHYYARTDAAVVFKRRDTATGRVDYVYHG
                     NDGTGMAWNDTAQIDFLKPEAREAVRERILHVARNFPIIRFDAAMVLAKKSVRRLWYP
                     EPGQGGDIPSRAERALSAEDFDRAMPEEFWREVVDACAREAPDTLLLAEAFWMMEGYF
                     VRTLGMHRVYNSAFMNMLKREENAKYRETVKNTQEFDKDILKRFVNFMNNPDEETAVA
                     QFGKGDKYFGVCTMMATMPGLPMFGHGQLEGFEEKYGMEYRRAYRDEAPDRELLERHE
                     REIFPLLKRRRLFSGVADFLLYDLVSGDGSVNENVFAYTNGDGGELALVAYNNAYAAA
                     SGRIRDSAPYAEKLPGGGRRAARRSLAEALALRGGDKRFLAMRELRSGLWFLRRSSEI
                     AGSGLALVLSGYECQVFLDLFELEDDARGTYAAVHDALAGRGTPDLAWAVQDVVLKDL
                     YAALAALATPSYFGLAALAVRGPAAE"
     assembly_gap    2914..3013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3014..3856)
                     /locus_tag="PLG14_05315"
     CDS             complement(<3014..3856)
                     /locus_tag="PLG14_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48588.1"
                     /translation="MDERAGERLSCGLAEGGGEAKSLVEGRSILASSRLGLVFDDYPL
                     LTAVLDRSRRLVYANRRALALMELEGEASVLGLRFGEAFGCIHAREAEGGCGTGLACR
                     FCGAARGIEEGLAGLPASEECNIARDREGKEDSLDLRIWAKPLELEGRGFILLAAEDI
                     SAEKRREILERVFYHDIANTASGIRNILDLLRFLPGEDPGEYLALARAAAEQLLDETE
                     SQRALKAAESRELAVRPVLLDGGEALKGAMDLFAYRLHGKEKRLALSPDSERPLLETD
                     PALLR"
     gene            3978..4658
                     /locus_tag="PLG14_05320"
     CDS             3978..4658
                     /locus_tag="PLG14_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX48589.1"
                     /translation="MSIAVEHEKRKREILEKALDVFVDEGYEDTTFQKIAERCGITRT
                     ILYLYFKNKREIFSFSIKLFTERLEASIRGVSDDPGLCQAEKLSAIAGMAIAECSRSR
                     RLLSVVLDYLGHTRAAGGNPDERVRRRTIRMRHIISGLLIEGIEKGEFRPCSVKAAGD
                     LFYALIESAIFRIAVLGREDAAGLAEAARLFAMGLRVDPSARVDPSAHTEPAAAQARD
                     QVAAGGAR"
     gene            4655..5548
                     /gene="lipA"
                     /locus_tag="PLG14_05325"
     CDS             4655..5548
                     /gene="lipA"
                     /locus_tag="PLG14_05325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOX48590.1"
                     /translation="MSPAPLRKPEWLKVAIPAGDNWRKTSELLKRRGLHTVCDEARCP
                     NKAECWGQATATFMILGAVCTRGCRFCAVATAKTGEPPRPEEPAELAKAVAELGLKYA
                     VVTSVDRDDLPDRGAGHFAAVVRAIREANPGTRVEVLTPDFREGEIEQVLDSGPAVFA
                     HNIEVVRRLQGVRDARASYEKSLETLRLAARDGRAVVKSSVMLGLGETEAELYEAMDD
                     LRAAGCSSLVLGQYLRPTPAEIEVVEYVAPEAFRRHADAARAKGFVSVVAAPLARTSY
                     HARAGFEEGSRAGAGDRGRSS"
     gene            5661..5948
                     /locus_tag="PLG14_05330"
     CDS             5661..5948
                     /locus_tag="PLG14_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48591.1"
                     /translation="MVTRLDYKIPGGKLLRVEVEFEGGVVLRAKVAGDFFAHPESDFE
                     AAEAELSRVPVDRLAEAALGAFSRPGLVLYGASASDIAETLRRAVDEAQVS"
     gene            5929..6759
                     /locus_tag="PLG14_05335"
     CDS             5929..6759
                     /locus_tag="PLG14_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOX48592.1"
                     /translation="MRLRFLDTGPGKGAFNMGLDEALLEAVAAGRSEPVLRLYTWDPA
                     AITIGYFQSAAAEVDLEAAREAGVDVLRRVTGGGAVFHAAEITYSLVVPEGHALAPAD
                     ILESYSRICGGLVEGLSLLGVEASFAPINDIVSGGKKISGNAQTRKRGCLLQHGTVLL
                     DVDPERMFSLLKVPSEKLKGKLIQDVKARVSGLKGLLGREVGYAEAADALGRGFARAF
                     GRLEAGPGRAAGTGAQFPEPVAALPAELLPSVPSPEELERARLLAAEKYSTSLWTMRR
                     "
     gene            complement(6788..7504)
                     /locus_tag="PLG14_05340"
     CDS             complement(6788..7504)
                     /locus_tag="PLG14_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOX48593.1"
                     /translation="MQPKINQVFAIILLASTTAFISISCGNEAQAFHPDIDSEIVRLA
                     ALSPSEIPGFMQNLDRSDIISEYYRDPATKQSVIQFFGELTHSDRIAAIILENADRYG
                     VPPGLAFALAYEESKFKVNAVNDNGDSLDRGIFQLNSKSFPDLGPSEVFDPAVNARYG
                     LAHLEFCLRTGGNEVAALAMYNAGSRRVTKDGTPRRTLDYIFRISKYEENIASLFAAK
                     IVAQDRIRVAKAFELGATRD"
     gene            7904..>8226
                     /locus_tag="PLG14_05345"
     CDS             7904..>8226
                     /locus_tag="PLG14_05345"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOX48594.1"
                     /translation="MDPQAIRVIVTGGTFDKHYDEIRGELTFKDSHLPEILKLTRVTL
                     PVEIEQDQLIDSLQMQDANRRSVLEACKRAPESRLLVTHGTDTMAETAKVLGPAGLDK
                     TIVLTG"
     assembly_gap    8227..8326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8610..9842
                     /locus_tag="PLG14_05350"
     CDS             8610..9842
                     /locus_tag="PLG14_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX48595.1"
                     /translation="MAFDEHDIRGIIQIDSELNKIQDSDLLLERILLEARRVVGAEAG
                     TIYVKDGDKLNFKYSQNALQERSLPPGQKLPYSAFSLQINEKTISGYVAMTGELLNIP
                     DVYKIPEGSPYGYNTYYDEKTHYKSTSMLTIPLKTSAGGLLGVIQVINAKDDAGNTIA
                     FSREDEILVTHFAANATVALQRAFMTKAMILRMIKMAELRDPKETGAHVNRVAGYAVE
                     IYDRWAFKRAVPEKERERYKDTLKLAAMLHDVGKVAISDLILKNPARFTPDEYKVMQA
                     HAYFGSLLFIDSQSELDRVASDIASIHHENWDGTGYPSTIAEWPDETIAAMARRSPRN
                     LAGERIPLGGRIVAVADVFDALVSRRVYKEAWDEEAVYEEIRKLSGTKFDPEVVEAFF
                     EVQANIEAIRARYPDSES"
BASE COUNT         1568 a         3302 c         3350 g         1472 t
ORIGIN      
        1 ctcggccgcg gggccgcgga cggccagggc cgcgaggccg aagtaggagg gcgtggcgag
       61 ggcggcgagg gcggcgtaga ggtccttgag gacgacgtcc tgcacggccc aggcgaggtc
      121 gggcgtcccc cggcccgcga gggcgtcgtg gaccgccgcg taggtgccgc gggcgtcgtc
      181 ctcgagctcg aagaggtcca ggaagacctg gcactcgtag cccgagagga cgagggcgag
      241 gcccgagccg gctatctccg acgagcggcg gaggaaccag aggccggagc ggagctcgcg
      301 catcgcgagg aacctcttgt cgccgccccg caaggccagg gcctcggcga ggctccgcct
      361 ggcggcgcgc ctgcccccgc ccgggagctt ctccgcgtag ggggcggagt ccctgatccg
      421 gcccgaggcg gccgcgtagg cgttgttgta ggcgacgagg gcgagttcgc ccccatcgcc
      481 gttcgtgtag gcgaagacgt tctcgttgac cgagccgtcg cccgagacga ggtcgtagag
      541 gaggaagtcc gcgacgcccg agaagaggcg gcggcgcttc aggaggggga agatctcgcg
      601 ctcgtgccgc tcgaggagct cccggtcggg agcctcgtcg cggtaggcgc ggcggtactc
      661 catgccgtac ttctcctcga agccctcgag ctggccgtgg ccgaacatcg ggaggccggg
      721 catcgtcgcc atcatcgtgc agacgccgaa gtacttgtcg cccttgccga actgggcgac
      781 ggccgtctcc tcgtcggggt tgttcatgaa gttgacgaag cgcttgagga tgtccttgtc
      841 gaactcctgg gtgttcttga cggtctcccg gtacttcgcg ttctcctcgc gcttgagcat
      901 gttcatgaag gccgagttgt agacgcggtg catgcccagg gtccggacga agtagccctc
      961 catcatccag aaggcctccg cgaggaggag ggtgtcgggc gcttcgcggg cgcaggcgtc
     1021 gacgacctcc cgccagaact cctcgggcat cgcgcggtcg aagtcctccg cggagagggc
     1081 ccgctccgcg cgcgagggga tgtcgccgcc ctgtcccggc tcggggtacc agaggcgccg
     1141 gacgctcttc ttcgcgagga ccatcgccgc gtcgaagcga atgatgggga agttccgcgc
     1201 cacgtggagg atgcgctcgc gcacggcctc gcgggcctcg ggcttgagga agtcgatctg
     1261 ggccgtgtcg ttccaggcca tgcccgtgcc gtcgttgccg tggtagacgt agtcgacccg
     1321 gcccgtcgcg gtgtcgcgcc gcttgaagac gacggcggcg tccgtccgcg cgtagtagtg
     1381 gtcctcgatc cggacgtcca cgcggccgtc gcccgagagg ttcccgctct cgaaggagta
     1441 gccggggaag gggcaggcct ccgcccgcat gaggagctcg ggccgctcgc gcacccaggc
     1501 cgagtcgatg ccgacgtggt tgggcaccat gtccgcggcc aggcggatcc cgcgcagggc
     1561 gcagcggccc ttgagcgact ccagggcggg ccagccgccg agctcccccg cgatctcgta
     1621 gtcgtagagg gagtaggcgg aggcggccgc ctcgggattg ccgcagcgcc gcttgatctc
     1681 cgcgctcgcc tggctccgct cccagaggcc gatgagccag aggccgttga agccgcgggt
     1741 cgcgaggagg tcgagctcct cgtcggggat ctggtcgagc cgcgccacct ccctgccgta
     1801 ggcgacggag agctggtgga gccagacgag gacgctcttg gccatcatca cgacgttggg
     1861 catccagtcc ttgtccggcg tgaagcgctc gtactccttc tccatgccgc ggtaggcgta
     1921 ggcccgcgtg gggccgggcc cggggaagcg cggcttctcc tcctcgcgga tgaggtccag
     1981 gccgccgagg aggcggagga gcttgtcccc gaccaggagg ccccagcggc gcctgatgta
     2041 gtcgagctgg ccgggcaggg agtagggcgc cgcgcggacg ggcgccctga ggagggtgag
     2101 gaggtcctgg tcgtcgggac cgaagcgggg gagggccgag aaggcccgct ccgcggcggc
     2161 catcgcggcg gcgtaggccg gcagggctgc gagggaggcg gggaggggcg cctcgcccga
     2221 tggccgcgag ccgtcgtcga agaggaagcg gtaggggccg aaggcggggt tctcgttcgc
     2281 gagcctgagg aggaggagct cctccagggc gaggatccgg ttcgggacgc cgtcccgcga
     2341 ggccgcgagc caggccttct ccgtcgcctc gcggcggtag acggcgaggg gcgggaagtc
     2401 ccgcacgaag gcggcaagga ggccgtccag ggcctccttg ccgagctcct tctcgagggc
     2461 ggagaggagg gcggggaagg cctccgccga ggcgcgctcg cggtagagcc gcgcgacgtc
     2521 gtggagtatc tcgtcgatga gggccatcgc gttcaggcgg cccgccctga cggcccgctc
     2581 ggggaggagg gcggcgtcga ccacctcgtt gatgcgcctc gcgaggaggc gtgcggccgc
     2641 gaagtcgggc aggacgacgt tgcccgtgga gcggaagagg gagcctgcga ggccgagctc
     2701 gtcgcgcgcg cggcgggaga cgtggaattc catgagcctg tcgccgggct tcgcctcgag
     2761 cccgccctcg ccctccaagc ccgcgagcct caggacgaga accttctctt catcgctcat
     2821 ggaacctccc tgcccgcgcc ggcccgtccc cgcgcgcctc tcgcgcgccc gccgggcccc
     2881 tttgggcgaa tactactcca ggcggggccg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnngcggagg agggcggggt cggtctccag gaggggccgc tcggagtcgg
     3061 gcgagagggc gaggcgcttc tccttcccgt ggaggcggta ggcgaagaga tccatggctc
     3121 ccttgagcgc ctcccccccg tccaggagga cgggccgcac ggcgagctcg cgggactcgg
     3181 cggccttgag ggcgcgctgg gactcggtct cgtcgaggag ctgctcggcc gccgcccggg
     3241 ccagggcgag atactcgccc ggatcctccc ccgggaggaa gcgcaggagg tccaggatgt
     3301 tccggatgcc ggaggccgtg ttcgctatgt cgtggtagaa gacccgctcc aggatctcgc
     3361 ggcgcttctc ggcggagatg tcctccgcgg ccaggaggat gaagcccctc ccctcgagct
     3421 cgagtggctt ggcccagatc cgcaggtcca aggagtcttc cttgccctcg cggtcccgcg
     3481 cgatgttgca ctcctcggag gccggcagcc ccgcgaggcc ttcctcgatc ccccgcgccg
     3541 cgccgcagaa gcggcaagcc aggcccgtcc cgcagccgcc ctcggcctcg cgggcgtgga
     3601 tgcagccgaa ggcctcgccg aagcggaggc ccaggacgga ggcctcccct tcgagctcca
     3661 tgagggcgag ggcccgccgg ttcgcgtaga ccaggcgcct cgagcgatcc aggacggcgg
     3721 tgaggagggg atagtcgtcg aagacgaggc cgaggcggga ggaggcgagt atgctccgcc
     3781 cttcgacgag gctcttggcc tcccctccgc cctccgcgag tccgcaggag agcctctcgc
     3841 cggctcgctc gtccatgccc taccttagcc ccgattccgg ccttcgggca aggaaaaagg
     3901 gctggtttcg gggagcttcg gagcttcacg aaggttgcgt tttgtcctat acttacacct
     3961 cgacgaaggg ggtgaaagtg tcaatcgccg tggagcacga gaaaaggaag cgggaaatcc
     4021 tcgaaaaggc cctcgacgtc ttcgtcgacg agggttacga ggacacgacc ttccagaaga
     4081 tcgccgagcg ctgcggcatc acgcgcacta tcctttatct ctatttcaag aacaagcgcg
     4141 agatattttc tttcagcata aagcttttta ccgagagact cgaagcctcg atccgcgggg
     4201 tctccgacga tcccgggctc tgccaggctg agaagctctc cgccatcgcc ggcatggcca
     4261 tagccgagtg ctcgaggagc cgcaggctcc tctccgtcgt cctcgactac ctgggccata
     4321 ccagggccgc gggaggcaat cccgacgagc gggtgcgccg ccgcacgatc cggatgcgcc
     4381 acatcatctc cggcctcctc atcgagggca tcgagaaggg cgagttcagg ccctgctccg
     4441 tgaaggcggc gggggacctc ttctacgccc tcatcgagtc cgcgatcttc cgcatcgccg
     4501 tcctgggccg cgaggacgcc gccggcctgg ccgaggccgc ccgcctcttc gccatgggcc
     4561 ttcgcgtcga tccctccgcg cgcgtcgatc cctccgcgca taccgagccc gccgccgcgc
     4621 aggccaggga ccaggtcgcc gcgggaggcg cccgatgagt ccggcccccc tgcggaagcc
     4681 cgagtggcta aaggtcgcca tcccggcggg cgacaactgg cgcaagacat ccgagctcct
     4741 taagcgccgc ggcctccaca ccgtctgcga cgaggcccgc tgccccaaca aggccgagtg
     4801 ctggggccag gcgaccgcga ccttcatgat cctgggcgcc gtctgcaccc ggggctgccg
     4861 cttctgcgcc gtcgcgaccg cgaagacggg cgagccgccg cggcctgagg agcccgcgga
     4921 gctcgcgaag gccgtcgccg agctcggcct caagtacgcc gtcgtcacga gcgtggaccg
     4981 cgacgacctt ccggaccggg gcgcggggca cttcgccgcc gtcgtccgcg cgatccggga
     5041 ggcgaatccc ggaaccaggg tcgaggtcct gacgcccgac ttccgcgagg gcgagatcga
     5101 gcaggtcctg gactcgggcc cagccgtctt cgcccacaac atcgaggtgg tgcggaggct
     5161 ccagggcgtg cgggacgcgc gggcctccta cgagaaatcc ctggagaccc tccgcctcgc
     5221 ggccagggac ggccgggccg tcgtcaagtc gagcgtcatg ctcggcctgg gcgagaccga
     5281 ggccgagctc tacgaggcca tggacgacct ccgcgccgcg ggctgctcga gcctggtcct
     5341 cggccagtac ctcaggccga cccccgcgga gatcgaggtc gtcgagtacg tcgcccccga
     5401 ggccttcagg cgccacgccg acgccgcgag ggccaagggc ttcgtgagcg tcgtcgccgc
     5461 gcccctggcc aggacctcct accacgcccg ggccggcttc gaggaaggaa gccgggccgg
     5521 ggccggcgac cggggccgat cctcatgata accaggctgg actacaagat ccccgcgcga
     5581 ggcgctcgag gcgcctcgcg gcgcggaatc ggaggccgat tccgcgatca gccgccagct
     5641 tcccgccggg gccgatccga atggtaacca ggctggacta caagataccc ggcgggaagc
     5701 tcctcagggt cgaggtggag ttcgaggggg gcgtcgtctt gcgggccaag gtcgccggcg
     5761 acttcttcgc ccaccccgag tccgacttcg aggccgccga ggccgaactc tcccgggttc
     5821 ccgtcgaccg cctcgcggag gcggccctcg gcgccttctc gaggcccggc ctcgtcctct
     5881 acggggcctc ggcctctgac atagccgaaa ccctaaggag ggccgtcgat gaggctcagg
     5941 tttcttgaca cgggccccgg caagggcgcc ttcaacatgg gcctggacga ggcccttctc
     6001 gaagccgtcg ccgcgggccg ctccgagccc gtcctgcgcc tctacacctg ggacccggcc
     6061 gcgatcacga taggctactt ccagtccgcg gcggccgagg tcgacctgga ggctgcccgg
     6121 gaggccggcg tcgacgtcct tcgccgcgtc acgggcggcg gggcggtctt ccacgccgcg
     6181 gagatcacct actccctcgt cgtccccgag ggccacgccc tggccccggc ggacatcctc
     6241 gagtcctatt cgcggatctg cggcggcctc gtcgagggcc tgtccctcct gggcgtcgaa
     6301 gcctccttcg cccccatcaa cgacatcgtc tccggcggga agaagatctc cggcaacgcc
     6361 cagacgcgga agcggggctg cctcctccag cacgggacgg tcctcctcga cgtggacccc
     6421 gagaggatgt tctcgctgct caaggtgccg agcgaaaagc tcaaggggaa gctgatccag
     6481 gacgtgaagg cgagggtctc ggggctcaag gggctcctgg gccgcgaggt cggctacgcc
     6541 gaggccgcgg acgccctcgg acggggcttc gccagggcct tcggccggct cgaggccggc
     6601 ccgggccgcg ccgccggcac cggggctcag ttcccggagc ccgtcgccgc gttgccagcc
     6661 gagctcctgc cctcggtccc gagccccgag gagctcgagc gggcgcggct cctcgcggcc
     6721 gagaagtact cgacctcgct ctggacgatg cggcgctgag gctcgcggcg tccggggccg
     6781 gggcttccta gtcccgagtc gcgccgagct cgaaggcctt cgcgacgcgg atcctgtcct
     6841 gcgcgacgat cttcgcggcg aagaggctcg cgatgttctc ttcgtacttc gagatccgga
     6901 agatgtagtc gagagtccgg cggggcgtcc cgtccttcgt gaccctccgc gagcccgcgt
     6961 tgtacatcgc gagggcggcg acctcgttcc cgcccgtgcg caggcagaac tctaggtggg
     7021 ccaggccgta gcgcgcgttc acggccggat cgaagacctc cgaggggccg aggtcgggga
     7081 aggacttcga gttgagctgg aagatgcccc gatccaggct gtcgccgttg tcgttgaccg
     7141 cgttcacctt gaacttgctc tcctcgtagg cgagggcgaa ggcgaggccc ggcggcacgc
     7201 cgtagcggtc ggcgttctcg aggatgatgg ccgcgatacg gtcggaatgg gtgagctccc
     7261 cgaagaactg gatgacgctc tgcttggtcg ccggatcccg gtaatactcg gaaatgatgt
     7321 cgctgcggtc gagattctgc atgaagccgg ggatctcgct cggcgatagg gcggccagcc
     7381 tgacgatctc cgagtcgatg tccgggtgga aggcctgggc ctcgttcccg caggaaatgg
     7441 agataaatgc tgttgtagac gctaataata taattgcgaa tacctggttg atcttcggtt
     7501 gcatgcggag gaaggactcc tttggtaaat aacatcaggg gctacctgag cagggcgcta
     7561 ttgtatcggt gagcgtttta tcgtcaagta gggaagatga cgactattgt caatatattt
     7621 agaatcgaaa atatgtagag atgaaatatt tcaggactgg gttatggcta gaaggaaata
     7681 tcaagtaatg aaaaaattgc gaaatggaag catataaaaa ttgtaccgct ctctttccgc
     7741 aacgcctcga ctaatcgatt ccttctatag ataaaggccc ccgtcggcgc acagagacag
     7801 aacccggcgt ccgcctccat ggtccgcggg tgctcccccg gggggggcct cgccgcctgc
     7861 ctccgcttgc caaagccagg gcgcggatgc atactcccct cccatggatc ctcaagccat
     7921 tcgcgtcatc gtaaccggcg gcaccttcga caagcactac gacgagatca ggggcgagct
     7981 gaccttcaag gacagccacc ttcctgagat cctcaagctg acgcgggtga ccctgcccgt
     8041 ggagatcgag caggaccagc tgatcgacag cctccagatg caggacgcga accgccgctc
     8101 cgtgctcgaa gcctgcaagc gggcccccga gagccggctt ctcgtcaccc acggcaccga
     8161 cacgatggcg gagacggcca aggtcctggg accagccggc ctcgacaaga ccatcgtcct
     8221 cacgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca tctctccccc
     8341 gtaggccggc ctggtcgcga cctgttcgtg gcccggcccg gcccggccca gctaacctgc
     8401 ccgcgggcgg gcttctcgag cctcgccgcg gcgagccaag cgcgccattt caatgcgtga
     8461 aagttggtta accccttatg ccgcaagccg agccgggtaa agaagggagc tcgttgtgcc
     8521 gaaggtattg gaggaagagg acgctctctc cgttgcgttc ctcctccgct gactttagac
     8581 tatgggtaga caagggataa ggacgcgcga tggcctttga cgaacacgat atccggggaa
     8641 tcatccagat agactcggaa ctcaacaaga tccaggactc cgacctcctt ctcgagcgta
     8701 tcctcctcga ggccaggcgg gtggtcggcg ccgaggccgg cacgatctac gtcaaggacg
     8761 gggacaagct caacttcaag tactcccaga acgccctcca ggagaggagc ctccccccgg
     8821 gccagaagct gccctactcg gccttcagcc tccagatcaa cgagaagacg atctccggct
     8881 acgtcgcgat gacgggagag ctcctcaaca tccccgacgt ctacaagatc cccgagggca
     8941 gcccctacgg ctacaacacc tactacgacg agaagacgca ctacaagtcg acctcgatgc
     9001 tcacgatccc cctgaagacc agcgccggcg gcctcctggg cgtcatccag gtgatcaacg
     9061 ccaaggacga cgcggggaac accatcgcct tctcccgcga ggacgagatc ctcgtcaccc
     9121 acttcgccgc gaacgcgacg gtcgccctcc agcgcgcctt catgaccaag gccatgatcc
     9181 tgcgcatgat caagatggcc gagctccgcg accccaagga gacgggcgcc cacgtcaacc
     9241 gcgtcgcggg ctacgcggtg gagatctacg accgctgggc cttcaagcgc gccgtccccg
     9301 agaaggagcg cgagcgctac aaggacaccc tgaagctcgc cgcgatgctc cacgacgtcg
     9361 gcaaggtcgc gatcagcgac ctcatcctca agaatccggc ccgcttcacc cccgacgagt
     9421 acaaggtgat gcaggcccac gcctacttcg gctccctcct cttcatcgac tcgcagtccg
     9481 agctcgaccg ggtcgcctcc gacatagcct ccatccacca cgagaactgg gacggcacgg
     9541 gctacccgag cacgatcgcg gaatggccgg acgagacgat cgccgcgatg gcccggcgct
     9601 cgcccaggaa cctcgcgggg gagcgcatcc ccctgggcgg ccgcatcgtc gccgtggccg
     9661 acgtcttcga cgccctggtc tcgcggcgcg tctacaagga agcctgggac gaggaagccg
     9721 tctacgagga gatccgcaag ctctccggca cgaagttcga ccccgaggtc gtggaggcct
     9781 tcttcgaggt ccaggcgaac atcgaggcga tccgggcccg ctaccccgac tccgagtcct
     9841 gagggcgggg cctccccgcc gcccgtccgc ggcgcttgac cgcgccccgc gg
//
LOCUS       DAOOSJ010000077         9817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4477_length_9817_cov_5.773612, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000077 DAOOSJ010000000
VERSION     DAOOSJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_9817_cov_5.773612"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLG14_05355"
     CDS             <1..316
                     /locus_tag="PLG14_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48596.1"
                     /translation="FAGAQVGDKDKIETYMERCVKSASEAQFTGFGMESEWWAKLQTF
                     TPDGKPDKQVYRVIQIWGIDKDSLKKQIDAILKNEAAAEPKTEQKQRAMDMVQQSFYE
                     GF"
     gene            466..1947
                     /locus_tag="PLG14_05360"
     CDS             466..1947
                     /locus_tag="PLG14_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48597.1"
                     /translation="MRRQLVAAAAALVLLASCATTAPAQQKKAPSWVLDTPKPDASNT
                     YFVGQASGPLADPAGATDGAAANLIASIMQYIGVKVSVDSTATARATLDSYAAEIKQS
                     VTTQATNRLAGFQIKEKFLLEDKKAGKVTVYVLAAYATADLEKEKRRIAAVFQEKEDA
                     VAKPEAEGKALLEAGRGYEAVRKFVEAAVAASGSDIDNADIKLERNVNNARTALSRIR
                     FDKQGEGYKGLLGQPFPEPFKLRLVSGEGAGAPGVAGANLLVSYQRKQGSRTVSKTES
                     AVTDATGLLSYAPPPPDFVGKAKLVVRVDFQSSVDLLDKLPAKYEALRQALAEELRAK
                     YVEIGYEVSSNARAVPIAVAIVDYDEAGKAVAGAQAQAGLIDALTKEKFSVRSGGLDS
                     GLVGGMDDDAILAAAKSLLAGKAERLAYGVARLESVRKDGAMFLATAKAAVKVVELSS
                     GRILYSAEKTATGLGADEAAARRAAYRELGAGAIGKDLLSSLP"
     gene            2116..3747
                     /gene="lnt"
                     /locus_tag="PLG14_05365"
     CDS             2116..3747
                     /gene="lnt"
                     /locus_tag="PLG14_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOX48598.1"
                     /translation="MPRKLPESLGLVCLSAAAFALAHPNPLFPRGLGALAFFFFLPAA
                     AALRGARPGRAALQGALFALVGYGLMDSWLAAYDPIAPLVVLPLTACYYAGLFAALAA
                     LGRAFPRWIWATWPFAWVGLEFAKTLGFLGYPYGILGYALYEHLPLAQAASAAGVFGL
                     SALALWPQALLAQELAARELAAQDEAGSPGAAGARGPCGTARAAGAARARGALVLGVL
                     ALCEAAGLARLALPLKAEGPRIELALVQPDLPSGAEGLAAYRAALDVLIGESRAALAS
                     GAELVAWPETAFVPSIDYHLRYRPDRGLYDLVRECRDFIAGSGADFLVGNDHGARGPG
                     GAGDRVDYNAALAFPKGGGGPEVYAKSRLVPFAEWFPFEGALPRLHAFFERKAGGFWE
                     PGPGPASLPLAGTRVAAPICFEDCFGEGVRAMAGDGVGLVLALVNDEWSRSEAAERQH
                     LACSVFRAIETGKPVARAANSGLTALVDGRGRILDELPPFERGALRAAVDCAPGARTA
                     YLAVGDLFARACLGLALGALAAAAIELAGRRKRRP"
     gene            complement(3803..4618)
                     /locus_tag="PLG14_05370"
     CDS             complement(3803..4618)
                     /locus_tag="PLG14_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOX48599.1"
                     /translation="MRPRIIPDEPRPLLFAHRGVSSEAPENTMAAFRLARDKGLGGVE
                     LDVHLTADGRLAVIHDHFTGRTAGAEGLEPGPGAKGRGLELERASWPELKRLDVGAWK
                     GRGYSGEGIPLLEELFEELGEALYFDIELKNGARSDYGLEAAVAKAIRGARGGRGLFG
                     RCVVCSFNPVSLARFKSLMPEVPTGMLWSGDAELPAYLRHGEGRFIGRTDFLKPAKEK
                     VKAASAFHWRRLGGYEVLPWTVDEPEAAKRLLALGCVGLVSNRPHELGVEVRR"
     gene            complement(4615..5190)
                     /locus_tag="PLG14_05375"
     CDS             complement(4615..5190)
                     /locus_tag="PLG14_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HOX48600.1"
                     /translation="MDDKEFLGLDLEGAKEYMLAYATSVKQYEKDIALARSEAELWRG
                     RVGLAESKGAAELAAGARAKAAEAEGRIAALEAERAGLAADLARLRERLPYLKARERS
                     VDPDRLLAELQLMTGELLDGDAPAAAAAPGADAPGAEGTAAAQAGAAGAGAPGAQRSE
                     ASLDRDLGKLEKAARAESALEELKRRMGGSA"
     gene            complement(5283..6656)
                     /locus_tag="PLG14_05380"
     CDS             complement(5283..6656)
                     /locus_tag="PLG14_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48601.1"
                     /translation="MKRTATLAALVLALAMAASPLFAQAPVVSEKKDVAVFALGYYGW
                     AIPFETLGNIDMEIQKVFVDLGRFNIIGVTQRLSSGGLEQFIATLKKAKEASFVMPEK
                     YQFGEAILTEGEFNKLVGAFIVAAPVVSNFSSGYNQKTGYWETSIKTNVTFIDVAAGG
                     TVMAISEIQTSGSDKQSQNKSIQGAIDSIPMQLQYEIRKVPAFQINTRVLAASGGEIK
                     MQLGANMGIKKGDEYAIIVGGMVEGFKDEREAGLVLVKDVGPEVSTGKVLYGNGSVAK
                     DAQLREIPRMGADFAPYLHIVTGRKLKVFSDADQVEEDGANTMIGFRMPLSRGFYDWR
                     PFATVQVPVNGVRTFLSAFIVPVNAIVGAEYNLYLGRLALTPYGGVGASYIYVSEAFT
                     GVSRDTSDTYIPHIGFQGYLSASYLINRDMKVYGEVGYEYWLSLASWLYSSYGGLSVG
                     GGVSFKL"
     gene            6969..>7858
                     /locus_tag="PLG14_05385"
     CDS             6969..>7858
                     /locus_tag="PLG14_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOX48602.1"
                     /translation="MPRSYLSAPVPIAALATAPGEAALAMVRASGAGSIELAAACFSR
                     PEALLSAPGHSLVFGRLVHPRTGEAADEVLASVFRAPRSFTGEDAVEFSCHGSPAAVR
                     AVLEALEAAGFAPALPGEFSFRAFANGKSDLVRSEALLELVRARSEGAREGALLRLEG
                     GLSRRLEAARAELLGLLAEVELRLDYAEEDGAPAGGPARPALEALRETLSALAASYRA
                     GRLYRDGARLVLAGGANAGKSSLFNLLLREERSIVSPEAGTTRDYVEAGVELAGFPLT
                     LLDTAGLREAGGAVEAEGVAR"
     assembly_gap    7859..7958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7959..8310
                     /locus_tag="PLG14_05390"
     CDS             <7959..8310
                     /locus_tag="PLG14_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOX48603.1"
                     /translation="SAGARAEPGEPSSGAAPPAGATSAASSAEPSAAAPGAPAAPSRA
                     ELRVASERQKLLLDRAVESLDAAFALQALGDEGLDALALELKEAAEALGEITGEIAAP
                     EILEAIFSRFCVGK"
     gene            8320..>9817
                     /gene="mnmG"
                     /locus_tag="PLG14_05395"
     CDS             8320..>9817
                     /gene="mnmG"
                     /locus_tag="PLG14_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOX48604.1"
                     /translation="MDYEAIVVGGGHAGIEAALALARLGRKTLLVTQDPETIGRMSCN
                     PAIGGLAKGNLVREIDALGGEMGRLIDASMLQYRTLNRSRGPAVQAPRAQADKARYAA
                     LARRAVEGEPRLFVRMDTAVDLLVSRDGKRVEGVLTSRGAAIGARAVVVAAGTFMEGR
                     LFIGSWSGPGGRLGEPAATGLGDALRRRGFPVGRLKTGTPARVARRSVDFSRLEEEKG
                     EEPRPFSFAGGRIDRPSVSCWAAYTNAETHRVIRENLGSSPLYGGAITGRGPRYCPSI
                     EDKVVRFPERERHQVFVEPEGEHTEELYLNGVSTSLPEAVQEAFIRTMAGLERAEIVR
                     PGYAVEYDYLDPLALRASLESKLLEGLFLAGQTNGTSGYEEAAAQGLLAGINAARRIS
                     GEPPLVLPRSESYIGVLVDDLTTLGTKEPYRMFTSRAERRLSLRCDTADRRLTPTGRA
                     VGLVDDGRWELFGRRARAVEEVAELLRSRRVSRAEADAEAALAPHVGEP"
BASE COUNT         1269 a         3579 c         3481 g         1388 t
ORIGIN      
        1 cttcgccggc gcccaggtcg gcgacaagga caagatcgag acctacatgg agcgctgcgt
       61 gaagagcgcc tccgaggccc agttcaccgg attcggcatg gagagcgagt ggtgggccaa
      121 gctccagacc ttcacccccg acggcaagcc cgacaagcag gtctaccgcg tcatccagat
      181 ctggggcatc gacaaggaca gcctcaagaa gcagatcgac gccatcctga agaacgaggc
      241 cgccgccgag cccaagaccg agcagaagca gcgcgccatg gacatggtgc agcagtcctt
      301 ctacgagggc ttctaagctt atcgccgcgc cccgcgtccg ccccgcgggc ccttgaggcc
      361 cgcgcccgtg gcggcgcgcc cgcgcggcgc ccttggcccg ccccgagggg ccccttccgg
      421 gggccggaag gggcgggccc ttctttacca aggagccgat cgaccatgcg cagacaactc
      481 gtcgcggcgg cagccgccct cgtcctccta gcctcctgcg ccacgacggc gccggcgcag
      541 cagaagaagg ccccttcctg ggtcctcgac accccgaagc ccgacgcctc caacacctac
      601 ttcgtgggac aggcctccgg ccccctcgcg gacccggccg gcgccaccga cggggcggcg
      661 gccaacctca tcgcctccat catgcagtac atcggcgtca aggtctccgt ggactcgacg
      721 gccaccgccc gcgcgaccct ggacagctac gccgcggaga tcaagcagtc cgtgacgacg
      781 caggcgacga accgcctggc cggcttccag atcaaggaga agttcctcct cgaggacaag
      841 aaggccggca aggtgaccgt ctacgtcctg gccgcctacg cgaccgccga cctggagaag
      901 gagaagcgcc gcatcgccgc cgtcttccag gagaaggagg acgccgtcgc gaagcccgag
      961 gcggagggca aggccctcct ggaggcgggc cgcggctacg aggccgtgcg caagttcgtg
     1021 gaggccgccg tcgccgcctc cggctccgac atcgacaacg cggacatcaa gctggagcgc
     1081 aacgtcaaca acgctaggac ggccctctcg cggatccgct tcgacaagca gggcgagggc
     1141 tacaagggcc tcctgggcca gcccttcccc gagcccttca agctcaggct ggtctccggg
     1201 gaaggagcgg gcgcgcccgg cgtcgccggg gcgaacctcc tcgtgagcta ccagcggaag
     1261 cagggctcgc gtacggtgtc gaagacggag agcgccgtca ccgacgcgac cggcctcctc
     1321 tcctacgcgc cgccgcctcc cgacttcgtg ggcaaggcga agctcgtcgt gcgggtcgac
     1381 ttccagtcct ccgtcgacct cctggacaag ctgccggcga agtacgaggc ccttcgccag
     1441 gccctggccg aggagctcag ggcgaagtac gtggagatcg gctacgaggt ctcctccaac
     1501 gcgcgagccg tgccgatcgc cgtcgccatc gtcgactacg acgaggccgg gaaggcagtc
     1561 gccggagccc aggcccaggc cggcctcatc gacgcgctca cgaaggagaa attctccgtg
     1621 cgctccggcg gcctggactc gggcctcgtg ggcggcatgg acgacgacgc gatcctggcc
     1681 gcggccaagt ccctgctcgc cggcaaggcg gagcgcctgg cctacggcgt cgcgaggctc
     1741 gagtccgtga ggaaggacgg cgccatgttc ctggcgacgg ccaaggcggc cgtgaaggtc
     1801 gtcgagctct cctccgggcg catcctctac tcggcggaga agaccgcgac gggcctgggc
     1861 gccgacgagg ccgccgcccg ccgcgccgcc taccgcgagc tcggagcggg ggccatcggg
     1921 aaggacctcc tttcgagcct gccctaagcc ctcgcccttt ccccgccgcg gccggccggc
     1981 cgcggggcgc gccgcccggc aggtccgggc ggctttttca tgggcgcgcg catcgcgggc
     2041 ggcgcgccgc ccgcggagcc gcttccgtcg cgggcggcag gacggcgtgc tatactcctg
     2101 cctcgaggtg gtcccatgcc gcgcaagctt cctgagagcc tcggcctcgt ctgcctgtcg
     2161 gcggcggcct tcgcgctcgc ccatccgaat cccctcttcc cgcgcggcct cggcgccctg
     2221 gccttcttct tcttcctgcc cgccgcagcc gccctgcgcg gggcgcggcc cggaagggcg
     2281 gccctgcaag gcgccctctt cgcccttgtc ggctacggcc tcatggactc ctggctcgcg
     2341 gcctacgacc ccatcgcgcc cctcgtcgtc ctgccgctga ctgcctgcta ctacgcgggc
     2401 ctgttcgccg cgctggccgc cctgggccgc gcctttccgc gctggatctg ggcgacctgg
     2461 cccttcgcct gggtgggcct cgagttcgcg aagacgctcg gcttcctggg ctatccctac
     2521 ggcatcctcg gctacgcgct ctacgagcac ctgcccctgg cccaggcggc ctcggccgcg
     2581 ggcgtcttcg gcctctcggc tctggcgctg tggccccagg ccctcctcgc gcaggaactc
     2641 gccgcgcgtg agcttgccgc gcaggacgag gctggatcgc ccggcgcggc gggagcgcgc
     2701 ggtccatgcg ggacagcccg cgcggccggc gcggcgaggg cgcgcggcgc cctcgtcctg
     2761 ggcgtcctgg ccctctgcga agccgcgggc ctcgcgaggc tggcccttcc cctgaaggcc
     2821 gaggggccga ggatagagct ggccctggtc cagcccgacc tgccgagcgg ggccgagggc
     2881 cttgccgcct atcgcgccgc cctggacgtc ctcatcggcg agagccgagc cgccctggcc
     2941 tcgggggcgg agctcgtcgc ctggccggag accgccttcg tgccctcgat cgactaccac
     3001 ctccgctacc gccccgaccg cgggctctac gaccttgtcc gcgagtgccg tgacttcatc
     3061 gcgggctcgg gcgccgactt cctggtcggg aacgaccacg gcgcgagggg gccgggaggg
     3121 gcgggcgacc gagtggacta caacgcggct ctcgccttcc cgaagggcgg cggcgggccc
     3181 gaggtctacg cgaagagccg cctcgtgccc ttcgccgagt ggttcccctt cgagggcgcc
     3241 ttgccccgcc tccacgcctt cttcgagagg aaggcggggg gcttctggga gccggggccc
     3301 gggccggcct ccctccctct cgcggggacg agggtcgcgg cgcccatctg cttcgaggac
     3361 tgcttcggcg agggagtgcg ggccatggcc ggcgatggcg tcggcctcgt cctggccctg
     3421 gtgaacgacg agtggtcgcg ctcggaggcg gccgagcgcc agcacctggc ctgttcggtc
     3481 ttcagggcca tagagacggg gaagccggtc gcgcgcgcgg cgaactcggg cctcacggcc
     3541 ctggtcgacg gccgcggcag gatcctggac gagcttccgc ccttcgagcg gggcgcgttg
     3601 cgcgccgccg ttgactgcgc gcccggcgcg aggacggcct atctcgccgt cggggacctg
     3661 ttcgcgcgag cctgcctggg cctggccctg ggcgccttgg cggccgcggc gatcgagctc
     3721 gcgggccggc ggaagcggcg cccttgacta aggccctccc ggcggcggcg ccggcccgcc
     3781 ggcggcctgc gcgcctgcgc ccttagcggc ggacttcgac gccgagctcg tggggccggt
     3841 tggagacgag accgacgcag cccagcgcga ggaggcgctt ggccgcctca ggctcgtcga
     3901 cggtccaggg caggacctcg tagccgccca gcctgcgcca gtggaaggcg gaggccgcct
     3961 tcaccttctc cttggccggc ttgaggaagt cggtcctgcc gatgaagcgg ccctcgccgt
     4021 ggcggaggta ggcgggcagc tcggcgtcgc ccgaccagag catccccgtc ggaacctcgg
     4081 gcatgaggct cttgaagcgc gcgagggaga ccgggttgaa agagcagacg acgcagcggc
     4141 cgaagaggcc gcggccgccc cgcgcgccgc ggatcgcctt cgcgacggcc gcctccaggc
     4201 cgtagtcgga cctggcgccg ttcttgagct cgatgtcgaa atagagcgcc tcgcccagct
     4261 cctcgaagag ctcctccagg agggggattc cctcgcccga gtagccgcgg cccttccagg
     4321 cgccgacgtc caggcgcttg agctctggcc aggaagcgcg ctcgagctcg aggcccctgc
     4381 ccttggctcc ggggcccggc tcgagcccct cggccccggc cgtccttccc gtgaagtggt
     4441 cgtggatgac cgcgagcctg ccgtcggccg tcaggtgcac gtcgagctcg acgccgccca
     4501 ggcccttgtc ccgggccagg cggaaggcgg ccatcgtgtt ctccggcgcc tcgcttgaga
     4561 cgccgcggtg ggcgaagagg aggggccggg gctcgtccgg gatgatgcgg ggcctcatgc
     4621 cgagccgccc atcctgcgct tgagctcctc cagggccgac tccgcccggg ccgccttctc
     4681 gagctttccg aggtcgcggt cgagggacgc ctcggagcgt tgcgcgccag gggcgcccgc
     4741 gcccgccgcg ccggcttggg cggcggccgt gccttccgcg cctggcgcgt ccgccccggg
     4801 cgccgcggcg gcggcgggag cgtcgccgtc caggagctcg cccgtcatga gctggagctc
     4861 ggcgaggagc ctgtcgggat cgacgctccg ctcgcgggcc ttgaggtagg gcaggcgctc
     4921 gcggaggcgg gcgaggtcgg ccgcgaggcc cgcgcgctcc gcctcgaggg cggcgatccg
     4981 cccttccgcc tccgcggcct tggcccgggc gcccgcggcg agctcggcgg cgcccttcga
     5041 ctcggccagg cccacccggc cacgccagag ctcggcctcc gagcgggcga gggcgatgtc
     5101 cttctcgtac tgcttcacgc tcgtcgcgta ggcgagcatg tattccttgg cgccctcgag
     5161 gtcgaggccg aggaactcct tgtcgtccat gggaaggctc ctttgcggat cgtggaaggc
     5221 cgcctatgaa aggggggatc atacgtctcc gcatgatccc cccgcttcgc ggcccggaag
     5281 tgctagagct tgaaggagac gccgccgccg acggagagtc cgccgtagct cgaatagagc
     5341 cagctcgcga ggctcagcca gtactcgtag ccaacctcgc cgtagacctt catgtcgcgg
     5401 ttgatgagat aggaggcgga gagatatccc tggaatccga tgtgcgggat gtaggtgtcg
     5461 gaggtgtccc gcgagactcc ggtgaatgcc tcgctcacgt agatatagct cgcgcctacg
     5521 ccgccgtagg gcgtgagtgc gaggcggccg aggtagaggt tgtactccgc gccgacgatc
     5581 gcgttgacgg gcacgatgaa ggcggaaagg aaggtccgca cgccgttgac cggcacctgc
     5641 acggtcgcga aggggcgcca gtcgtagaat ccgcgcgaga ggggcatccg gaagccgatc
     5701 atggtgttcg cgccgtcctc ttctacctgg tcggcgtcgc tgaagacttt aagcttacgt
     5761 cccgtgacga tatggaggta gggcgcgaag tcggcgccca tcctcgggat ctcgcggagc
     5821 tgcgcgtcct tggcgacgga gccgttgccg tagagcacct tgcccgtgga gacctcgggg
     5881 ccgacgtcct tcacgagcac aaggcccgcc tcgcgctcgt ccttgaagcc ctcgaccatg
     5941 ccgccgacga tgatggcgta ctcgtcgccc ttcttgatgc ccatgttcgc gccgagctgc
     6001 atcttgatct cgccgccgga agccgcgagg acgcgggtgt tgatctggaa ggcgggcacc
     6061 ttgcggatct cgtactggag ctgcatcggg atcgagtcga tggcgccctg gatggacttg
     6121 ttctggctct gcttgtcgga gccggaggtc tggatctcgg agatcgccat caccgtgccg
     6181 cccgcggcca cgtcgatgaa ggtgacgttg gtcttgatcg aggtctccca gtacccggtc
     6241 ttctggttgt agccagagga gaagttggag acgacgggcg ccgcgacgat gaaggcgccg
     6301 acgagcttgt tgaactcgcc ctcggtgagg atggcctcgc cgaactggta cttctccggc
     6361 atcacgaagc tcgcctcctt ggccttcttg agcgtcgcga tgaactgctc gagcccgccg
     6421 gaggagaggc gctgggtgac gccgatgatg ttgaagcggc cgaggtcgac gaagaccttc
     6481 tggatctcca tgtcgatgtt gccgagggtc tcgaagggga tggcccagcc gtagtagccg
     6541 agggcgaaga cggccacgtc cttcttctcg ctgacgacgg gcgcctgcgc gaagaggggc
     6601 gaggcggcca tggcgagggc taggacgagg gcggcgagag tcgccgtgcg tttcatggtt
     6661 cctccttagg tcttgcgggg aagggcgacg gcccgtcgcc tcgcggccat aggcccgcga
     6721 gaaaccccga ctcctcattt tccgccctgc ggcggcccct gtcaatagaa gccgccctgc
     6781 gcggccctcc ctgcgccgtg atttgcgtcg cccccagcgg ggctttcccg ggccgcgtcc
     6841 ggaggcgctc cgcgccgtgg ccctacgcgc cgccacgccg cgcccgcgcc gcggcaaggc
     6901 cgagcctagc tcgccgcgcc gcggccttgc cgcgagggcc ccgcgcgggc tagcatgggc
     6961 caccgaccat gccgcgaagc tatctctccg cccccgtccc gatcgccgcc ctcgcgaccg
     7021 cccccggcga ggcggccctg gccatggtgc gcgcctcggg ggccggctcc atcgagctcg
     7081 cggccgcctg cttctcccgt cccgaggccc tgctctccgc ccccggccac agcctggtct
     7141 tcgggcgcct cgtccatccc cggaccggcg aagccgccga cgaggtcctc gcctcggtct
     7201 tccgcgcgcc caggagcttc acgggcgagg acgcggtgga gttctcctgc cacggctctc
     7261 ccgccgccgt ccgcgccgtc ctcgaggccc tggaggcggc gggcttcgct ccggcccttc
     7321 ccggcgaatt ctccttccgc gccttcgcga acggcaagtc cgacctggtc cgctcggagg
     7381 ccctcctgga gctcgtccgc gcccgctcgg agggcgcgcg cgagggcgcc cttctccgcc
     7441 tggagggcgg gctctcccgc cgcctcgagg ccgcgagggc cgagctcctc ggcctcctgg
     7501 ccgaggtcga gctccgcctg gactacgcgg aggaggacgg cgcccctgcg ggcggcccgg
     7561 cgcgccccgc cctcgaggcc ctgcgggaga cgctgtccgc cctcgccgcg agctaccgtg
     7621 ccggccgcct gtaccgcgac ggcgcccgcc tcgtcctcgc aggcggggcc aacgccggca
     7681 agtcgagcct cttcaacctc ctgctccgcg aggagcggtc catcgtgagc cccgaggcgg
     7741 ggacgacccg cgactacgtg gaggccgggg tcgagctcgc gggcttcccc ctgactctgc
     7801 tcgacacggc cggtctccgc gaggcggggg gcgcggtcga ggccgagggc gtggcgcgnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cggccggcgc gcgcgccgag
     7981 cccggcgagc cgtccagcgg cgcggcgccg ccggccgggg cgaccagcgc ggcttccagc
     8041 gccgagccct ccgcggcggc gccgggcgcc ccggccgctc cgtcccgcgc cgagctccgc
     8101 gtcgcgagcg agcggcagaa gctcctcctc gaccgcgccg tcgagtccct ggacgccgcc
     8161 ttcgccctcc aggccctggg cgacgaaggc ctggacgccc tggccctgga gctcaaggag
     8221 gcggccgagg ccctgggcga gatcacgggc gagatcgccg cgccggagat cctcgaggcg
     8281 atcttctcgc gcttctgcgt cgggaaatag gagggccgct tggactacga agcgatcgtc
     8341 gtgggcggcg gccacgccgg gatcgaggcc gccctcgccc tggccaggct cgggcgcaag
     8401 accctcctcg tcacgcagga ccccgagacc atcgggcgca tgtcctgcaa tcccgcgata
     8461 ggcggcctgg ccaagggcaa cctggtccgc gagatcgacg ccctgggcgg cgagatgggg
     8521 cggctcatcg acgcctccat gctgcagtac cgcacgctca accgctcccg ggggccggcc
     8581 gtccaggcgc cccgcgccca ggccgacaag gcgcgttatg cggccttggc caggcgcgcg
     8641 gtcgagggcg agcctcgcct ctttgtgcgg atggacacgg ccgtcgacct cctcgtctcc
     8701 agggacggga agcgggtcga gggcgtcctc acctcgcggg gcgcggcaat cggcgcccgc
     8761 gccgtcgtcg tggcggcagg caccttcatg gaggggcggc tcttcatcgg ctcctggagc
     8821 ggccccgggg ggcggctggg ggagcccgcg gcgacgggcc tgggcgacgc cctccggcgc
     8881 aggggcttcc ccgtgggcag gctcaagacg ggcacgccgg cgagggtggc gaggcgctcg
     8941 gtggacttct cccggctcga ggaggagaag ggcgaggagc cgcggccctt ctccttcgcg
     9001 ggcggccgga tcgaccggcc ctcggtctcc tgctgggcgg cctacacgaa cgcggagacc
     9061 catcgggtca tcagggagaa cctgggctcc tcgccgctct acggcggcgc gatcacgggc
     9121 cgggggccgc gctactgccc ctcgattgag gacaaggtgg tgcgcttccc cgagcgggag
     9181 cggcaccagg tcttcgtcga gcccgagggc gagcacaccg aggagctcta cctcaacggc
     9241 gtctccacct ccctgcccga ggccgtccag gaggccttca tccgcacgat ggccggcctg
     9301 gaacgggcgg agatcgtccg gccgggctac gcggtcgagt acgactatct cgaccccctg
     9361 gccctgcgcg cctcgctcga gtcgaagctg ctcgagggac tcttcctcgc gggccagacg
     9421 aacggcacct cgggctacga ggaggcggcc gcccagggcc tcctcgcggg gatcaacgcg
     9481 gccaggcgga tctcgggcga gccgcccctc gtcctcccgc ggtcggagtc ctacatcggc
     9541 gtcctcgtgg acgacctcac gaccctgggg acgaaggagc cctaccgcat gttcacgagc
     9601 cgcgccgagc ggcggctatc gctccgctgc gacacggccg accgccgcct cacgccgacg
     9661 gggcgcgcgg tcggcctcgt ggacgacggg cgctgggagc ttttcgggcg gcgggcccgg
     9721 gctgtggagg aggtggccga actcctgagg tccagacgcg tgagccgcgc cgaggccgac
     9781 gcggaggcgg ccctcgcgcc ccacgtgggc gagcccc
//
LOCUS       DAOOSJ010000078         9751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4516_length_9751_cov_5.048989, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000078 DAOOSJ010000000
VERSION     DAOOSJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4516_length_9751_cov_5.048989"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLG14_05400"
     CDS             complement(<1..844)
                     /locus_tag="PLG14_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOX48605.1"
                     /translation="MKTDDFSFELPEELVAQFPPERRGESRLLVLDRASGGLRHAFVR
                     DLADHVEPGTLMVFNDSRVRKARIHGEALDTGARVEFLLLRRLGADLWEASCTKMKRQ
                     RPGRRYRFPDGVEAEIVGPAGPSAATGPLAAAGGPGAAGGPAGQAGPEGGRDETRVIR
                     FSPPVDEAWLDRFGHIPLPPYIRREDAPADAERYQTVYARQAGSAACPTAGLHFTEEI
                     LASLDAKGVERAYVTLHVGLGTFLPVRAEEIEGHAMHEEEFSVPEETARAVMRAKAEG
                     RPVLA"
     gene            complement(819..1961)
                     /gene="ruvB"
                     /locus_tag="PLG14_05405"
     CDS             complement(819..1961)
                     /gene="ruvB"
                     /locus_tag="PLG14_05405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOX48606.1"
                     /translation="MAATRKRSAARGDGTATEAGGDFPAGTLDAEASGAEALGAEARG
                     RRLDPAYDPSEDERETGLRPKVLSEFQGQARLKENLSLFITAARGREEPLDHLFLMGP
                     PGLGKTTLAQVVANELGVDWKPTSAPALDKPKDLAGVLTTLRERSVLFIDEIHRLKPA
                     IEELLYIAMEDFELDWVIGQGPAARTVRVPVPPFTLIGATTKSGNVSAPLSSRFGISL
                     RFGYYEPRELEDVIRRSSRILGVSVDAEAARLLAASSRGTPRIANRLLRRMRDFAQVG
                     GKASVTRTVVEEGLSRLEVDRLGLERLDRDILRAIITKYGGGPVGAETLAITVGEGVD
                     SLEDYCEPYLIQAGLIARTPRGRVALPAAYAHLGMEPPREDGRFLF"
     gene            complement(1965..2564)
                     /gene="ruvA"
                     /locus_tag="PLG14_05410"
     CDS             complement(1965..2564)
                     /gene="ruvA"
                     /locus_tag="PLG14_05410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOX48607.1"
                     /translation="MFNSIRGRLSEKRADSICLETGGVEWELSVPARSVDDFGVLGGE
                     VRAFAWLHHYEDGMRLYGFPTEADRAVFLELMKVEGIGPKQAMRILSGIRPEALDAAL
                     ESDDLAGLQRIPGVGPKLAQKMLLALKGKLVRPEEARPGRPASGGEWADVVRALADMG
                     YDRRAAEKSVAAKAAEVPAGPEKEREIFRRALLDLSTGA"
     gene            complement(2601..4085)
                     /locus_tag="PLG14_05415"
     CDS             complement(2601..4085)
                     /locus_tag="PLG14_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48608.1"
                     /translation="MDARDRDDIRLVYSVLAELGEAPSRPFPDSVARPVERPGSYAFP
                     SGAAAGAPREGAPRPVIVGSGPAGLFCALLLAERGFRPLVLERGDDVEARHKAVGAFF
                     ASGSLDPESNIQFGEGGAGTYSDGKLNTTVSDTLYRNAKVLEEFVEAGAPPEIRYLSK
                     PHIGTDYLVRVVKALREKIEALGGEVRFRSRVTSLLSGEGRVRGVVVGGTGAIEAETV
                     VLAIGHSARDTFLELSREGLELERKAFAIGLRIEHPQEMVSRRQYGESWRHPRLPVAD
                     YKLTHRSSDGRGVYTFCMCPGGHVVNASSEPGMVACNGMSDFRRDSRNANSAVVVAVR
                     PEDFGGDGVLAGIEFQRRWERLAFEAGGGGHALPVQTFGDFQAGRASKALGAVEPCAK
                     GAYALSELNACLPAFAARAIKEGILAFDRKLRGFARPDAVLTGVETRTSSPLRILRGE
                     DLQAPSLRGLYPCGEGAGYSGGIMSSAIDGIKVAEAVAAGAARG"
     gene            complement(4247..4699)
                     /gene="ruvC"
                     /locus_tag="PLG14_05420"
                     /pseudo
     CDS             complement(4247..4699)
                     /gene="ruvC"
                     /locus_tag="PLG14_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
     gene            complement(4827..5564)
                     /locus_tag="PLG14_05425"
     CDS             complement(4827..5564)
                     /locus_tag="PLG14_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX48609.1"
                     /translation="MSGHSKWATIKHKKGALDAKRGQMFTKLIKEISIAARMGGGDPD
                     GNPRLRTAVLKARAANMPKDNVDRAIKKGTGELEGVNYEELTYEAYAPGGGAILIEVL
                     TDNKNRAAAEIRNILTRSGSNLGATGSVSYLFKRKGVLSYDGEKYTEDKIMEAAIEAG
                     ADDVQNEDGELVVYTDPNAFETVLNAMNAAGFETDGAEISMVPETYITPDKDNATKVQ
                     KLIDRLEECEDTQNVYHNIELPEDLDE"
     gene            complement(5880..6122)
                     /locus_tag="PLG14_05430"
     CDS             complement(5880..6122)
                     /locus_tag="PLG14_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48610.1"
                     /translation="MKVLYCDVCKKPIENPTPTRNYYHIADIDICEPCKDELELAMKY
                     TVRSKKPFDYGWHDELTMKILHDGMQRGRIDVRVKR"
     gene            6207..7319
                     /locus_tag="PLG14_05435"
     CDS             6207..7319
                     /locus_tag="PLG14_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOX48611.1"
                     /translation="MHNDFRVAGTAYGPRSPLVVAEIGTSHGGDLAKAFELVDAAAEA
                     GADCAKFQCVFAEEIVHPNTGVVSLPGGPTPIFERFRDLELGADFYAAMKERAEARGM
                     LFLCTPFGLKSARLLRRLGVSAMKVASPELNHLPLIDELASYGLPVLVSSGVSTLADI
                     ERALRRLACPVLLLHCVTAYPAPAEEYNLRVLAGLSSVFGLPTGVSDHSADPVLVPSL
                     AVASGASCVEKHICLSRVDKGLDDPIALPPEDFARMARAVREAAELPAQATIAALSER
                     FGADVVEKVLGDGVKRLAPSEAGNYSRTNRSIHALRAIKKGEVYSAENLALLRTEKIL
                     RPGLGPELLPAVLGRKARRAVPSGEGITWEDVGDIG"
     gene            complement(7395..>9751)
                     /locus_tag="PLG14_05440"
     CDS             complement(7395..>9751)
                     /locus_tag="PLG14_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOX48612.1"
                     /translation="EALEALRAARLKAQSAPRPAAAQADAGAAAPGPAAGAAAPAATG
                     ASSSGAAQAVAGPAEAPGAEAQREARWSEAEGLLLDGLLYGAPDLAPLSRIVFKVVNK
                     DGSLRDLPELRQARDRIARAHRDIASITDSYFKNPEVRAMLQSDAPTQRDGRTVLAIR
                     ANFRGRVKGIVHEVSATGQTVFVEPEDLVQKNNELVEEEARYRQELQRILRETTERLL
                     PEAPALAAARRAMGGFDGIYARARHARLGGLVLARDLAEGFTIRKARHPLIGRRAVPI
                     DVELPAGTRTLIVTGPNTGGKTVTLKTVGLLALMNQFGLALPAAPESGFAVFDAVYAD
                     IGDEQSIDQSLSTFSGHMRVIGGIANDAGPRSLVLLDELGSGTDPEEGCAVAMGLLDW
                     FIGRGCLTVLTTHHGILKNYGYTRPGCLNASMDFDAASLQPTYRVLMGVPGESRALEV
                     ASQHGLRSEIIEGARKYLAEERADVSELIKGLTEKHRKLEDLESERRSRLKEAREEQR
                     KADLSRLKLRQRELELREQGVSDIRRLLGESRKTLENLVKEIREGEGSLGSDKTRAVK
                     DFLAGFAEAVEGEERRLESAREEAAEEARGAELADRAARAASEAAGGPEPGKRKAGAP
                     GGARAPAPLVLEEGAAVLVNPGKKRGVVLRRAKKGYWLVETESIRLTVPESKLELVPE
                     AAAAKMQVAVELAPRGEGGRTTAAFELDLRGFRLAEALEAVEKQIDAASLQGLSLFSI
                     IHGTGEGILGKGIHDYLRRQPAVAEYHFARPEEGGYGKTVVRLK"
BASE COUNT         1259 a         3419 c         3532 g         1541 t
ORIGIN      
        1 cggcgaggac gggccggccc tcggccttgg cgcgcatgac cgcgcgggcc gtctcctccg
       61 gcaccgagaa ctcctcctcg tgcatcgcgt gcccctcgat ctcctccgcc cggacgggga
      121 ggaaggtccc gaggccgacg tggagggtca cgtaggcgcg ctccacgccc ttcgcgtcca
      181 gggaggcgag tatctcctcg gtgaagtgga ggccggccgt cgggcaggcc gccgagcccg
      241 cctgccgcgc gtagacggtc tggtagcgct ccgcgtccgc gggcgcgtcc tcgcgcctga
      301 tgtagggcgg caggggtatg tggccgaagc ggtcgagcca ggcctcgtcg acgggggggc
      361 tgaagcggat gacgcgggtc tcgtcgcggc cgccctcggg gccggcctgg ccggcgggcc
      421 cgccggccgc gccgggcccg ccggccgccg ccaagggccc cgtcgccgcg gaaggtccgg
      481 ccgggccgac gatctcagcc tcgacgccgt ccgggaagcg gtagcggcgc cccggcctct
      541 gccgcttcat cttggtgcag gaggcctccc agagatcggc gcccaggcgc cttaagagga
      601 ggaactcgac gcgggctccc gtgtccaggg cctcgccgtg gatcctcgcc ttcctgaccc
      661 gcgagtcgtt gaagaccatg agggtcccgg gctcgacgtg gtccgcgagg tcgcggacga
      721 aggcgtgccg taggccgccc gaggcccggt ccaggacaag gaggcgggac tccccgcggc
      781 gctcgggcgg gaactgggcc acgagctcct ccgggagctc aaaagagaaa tcgtccgtct
      841 tcacgcgggg gctccattcc gaggtgggcg taggccgcgg gcagggctac gcggccgcgc
      901 ggcgtgcggg cgatgaggcc cgcctggatg agatagggct cgcagtagtc ctcgaggctg
      961 tccaccccct cgcccacggt gatggcgagg gtctcggcgc cgacggggcc cccgccgtac
     1021 ttcgtgatga tggcgcgcag gatgtcgcgg tccaggcgct cgaggcccag gcggtctacc
     1081 tcgaggcggg agagcccttc ctcgacgacg gtccgcgtga ccgaggcctt cccgcccacc
     1141 tgggcgaagt ccctcatgcg ccttaagagg cggttcgcga tacgaggcgt cccccggctc
     1201 gaggcggcga ggaggcgggc ggcctccgcg tccacggaga cgcccaggat acgcgaggag
     1261 cggcggatga cgtcctccag ctcgcggggc tcatagtagc cgaagcgcag ggagatgccg
     1321 aagcggctcg acaggggcgc ggagacgttg cccgacttgg tcgtggcgcc gatgagggtg
     1381 aagggcggga cggggacgcg gaccgtgcgc gccgccggcc cctggccgat cacccagtcg
     1441 agctcgaagt cctccatcgc gatgtagagg agctcctcga tcgcgggctt gaggcggtgg
     1501 atctcgtcga tgaagaggac ggacctctcg cgcagggtcg tgaggactcc cgcgaggtcc
     1561 ttgggcttgt ccagggccgg cgcgctcgtc ggcttccagt cgacgccgag ctcgttggcg
     1621 accacctggg ccagggtcgt cttccccagg cccggcgggc ccatgaggaa gaggtggtcc
     1681 aggggctctt cccgcccgcg ggccgccgtg atgaagaggg agaggttctc cttgagccgg
     1741 gcctggccct ggaactcgga gaggaccttg gggcggaggc ccgtctcgcg ctcgtcctcg
     1801 gagggatcgt aggcggggtc gaggcgccgc ccccgggcct cggcgccaag ggcctcggcg
     1861 cccgaagcct ccgcgtccaa ggttccggcg gggaagtccc cgccggcttc ggtagcggtc
     1921 ccgtcgccgc gggccgcgct cctcttgcgt gtcgctgcca tgccctaggc ccccgtcgag
     1981 aggtcgagga gggcgcggcg gaagatctcg cgctccttct cggggccggc ggggacttcc
     2041 gccgccttcg cggcgaccga cttctcggcg gcccggcggt cgtagcccat gtccgccagg
     2101 gcgcggacca cgtcggccca ctcgcctccg gaggcgggcc tgcctggccg cgcctcctcg
     2161 gggcggacga gcttcccctt gagggccagg agcatcttct gcgcgagctt ggggccgacg
     2221 cccggaatgc gctggaggcc ggccaggtcg tcggactcca gggcggcgtc cagggcctcg
     2281 ggcctgatgc cggagaggat gcgcatcgcc tgcttggggc cgatgccctc gaccttcatg
     2341 agctccagga agacggcgcg gtcggcctcc gtcgggaagc catagaggcg catgccgtcc
     2401 tcgtagtggt ggagccaggc gaaggcgcgg acctcgccgc ccaggacgcc gaagtcgtcg
     2461 accgagcggg cgggcacgga gagctcccat tcgaccccgc ccgtctccag gcagatcgag
     2521 tcggcccttt tctcgctgag cctgccgcgg atgctgttga acatggtggc tcgagtatac
     2581 cccggggcgg ccgccctagg ctatccgcgc gcggcgccgg cggccaccgc ctcggcgacc
     2641 ttgatgccgt cgatggccga ggacatgatc cctcccgagt agcccgcgcc ctccccgcag
     2701 gggtagagtc cgcgcaagga gggggcctgg aggtcctcgc ccctgaggat ccgcagcggc
     2761 gaggaggtcc gcgtctcgac gcccgtgagg acggcgtccg gcctcgcgaa gcccctgagc
     2821 ttccggtcga aggcgaggat gccctccttg atcgccctcg cggcgaaggc ggggaggcag
     2881 gcgttgagct cggagagggc gtaggcgccc ttggcgcagg gctcgacggc gcccagggcc
     2941 ttcgaggccc tccccgcctg gaagtcgccg aaggtctgga cgggaagggc gtggccgccg
     3001 ccgcccgcct cgaaggccag gcgctcccag cggcgctgga actcgatgcc cgcgaggact
     3061 ccgtccccgc cgaagtcctc cgggcgcacg gccacgacga cggcggaatt cgcgttccgg
     3121 gagtcgcgcc tgaaatcgct catcccgttg caggcgacca tgccgggctc ggagctcgcg
     3181 ttgacgacgt ggccgcccgg gcacatgcag aaggtgtaga ctccccgccc gtccgagctc
     3241 cggtgggtga gcttgtagtc ggccacgggg aggcggggat gccgccagct ctcgccgtac
     3301 tggcggcggg agaccatctc ctgcgggtgc tctatcctga ggccgatagc gaaggccttc
     3361 cgctcgagct cgaggccttc ccgggagagc tccaggaagg tgtcgcgggc cgagtggccg
     3421 atggcgagga cgacggtctc cgcctcgatc gccccggttc cgccgacgac gacgccgcgg
     3481 acccgccctt ccccggaaag gagggaggtc acccggctcc tgaagcggac ctcgccaccg
     3541 agggcctcga tcttctccct gagggccttc acgacgcgca cgaggtagtc cgtgccgatg
     3601 tggggcttcg agaggtagcg gatctccgga ggggcgcccg cctcgacgaa ctcctcgagg
     3661 accttcgcgt tcctgtaaag ggtgtcggag accgtcgtgt tgagcttccc gtcggagtag
     3721 gtgccggccc cgccctcgcc gaactggatg ttggactcgg ggtcgaggct ccccgaggcg
     3781 aagaaggcgc ccaccgcctt gtggcgggcc tcgacgtcgt cgccgcgctc caggaccagg
     3841 gggcggaagc cccgctcggc gaggaggagg gcgcagaaga ggcctgcggg gccgcttccg
     3901 acgatcactg gccgcggggc tccttcccgc ggcgcgccgg ccgcggcgcc cgaggggaag
     3961 gcgtaggagc cgggcctctc gacggggcgg gccacggagt cggggaaggg ccgcgacggc
     4021 gcctccccga gctcggccag gacggagtag acgaggcgga tgtcgtccct gtcccgcgcg
     4081 tccacggatt tcctctcgat ggagaggctc tttaagtccg tcgcgccgac cccgagggcc
     4141 cgcgctacgc gtccttcgag ctcggcctct tcgcagcgga gcgggagctt gacttgggga
     4201 agccggtaga ggggcaggtc gaacctccta ggagcggtcc ttcgtggttg gcccggcaga
     4261 tggccgcggc cagggcgtcg gccgcgtggt cgggcttggg tacggacttg aggcccagga
     4321 tgagccgcac catctcctgc acctgggcct tctccgcccg ggccgagccc gagacggcct
     4381 gcttgatcgc cgtcgggctg tactcgacga gttctatccc gcccttcgtg aaggcgaggc
     4441 ggatcacgcc cttggcctcg gagacgggga tggccgagct cacgttcttc cagaaataga
     4501 ggctctccat gcccgctgcc cgcggctccc attcctcgat gaggccggcc acgcggtcga
     4561 agatggagag gaggcgctcg cccgtcggct cgcccgagcc cgtcgagatg cagccgtggg
     4621 ccaggtggac gaggcggccg cccgactgct cgaggacgcc ccagcccacg gcggcgagcc
     4681 ccgggtcgat gcctataatg cgaagggccg ggcctccggc tccgcgcccc gcgcggcccg
     4741 ccttcgcccc ggcccccgtc cggccttcgc cggactcccg gagggcgccg ccgtcgcggc
     4801 ccgcctccgg ctcgccccgc tcgggcttac tcgtcgagat cctcggggag ctcgatgttg
     4861 tggtagacgt tctgggtgtc ctcgcactcc tcgagcctgt cgatgagctt ctggaccttc
     4921 gtggcgttgt ccttgtccgg cgtgatgtag gtctcgggga ccatggagat ctccgcgccg
     4981 tcggtctcga agccggccgc gttcatcgcg ttgagcacgg tctcgaaggc gttggggtcg
     5041 gtgtacacga cgagctcgcc gtcctcgttc tggacgtcgt cggcgccggc ctcgatggcg
     5101 gcctccatga tcttgtcctc ggtgtacttc tcgccgtcgt aggagaggac gcccttcctc
     5161 ttgaagaggt aggagacgga gcccgtggcg ccgaggttgg agccggagcg ggtgaggatg
     5221 ttgcggatct cggccgcggc gcggttcttg ttgtcggtga ggacctcgat gaggatggcg
     5281 ccgccgccgg gggcgtaggc ctcgtaggtg agctcttcgt agttgacgcc ctcgagctcg
     5341 cccgtgccct tcttgatggc gcggtcgacg ttgtccttgg gcatgttcgc ggcgcgggcc
     5401 ttgaggacgg ccgtgcggag gcgggggttg ccgtcggggt cgccgccgcc catgcgggcc
     5461 gcgatggaga tttccttgat gagcttcgtg aacatctggc cgcgcttcgc gtcgagcgcg
     5521 cccttcttgt gctttatcgt cgcccattta ctgtggccgg acatatgctc tccttgcctc
     5581 ccgaaacggc tcttgcccag ggcccgaaat caagcattcg gacccgcctg aggcccccga
     5641 atcgagtctg acggattctc agtgtacgga taacgatagc ataatcgaga aaatactgtc
     5701 aactatccga cgcttttcga cgattggtcg gtaagactag cgctatcttg cgcttttcca
     5761 gaatatcccg cacccggtcc cggatttgca atacaatgga aaaggccgcc tcaaggcggc
     5821 ctttcgcgcg aagcatggcg agagaggtcg gcgcggccgc gcctccctgg atcaggcctc
     5881 tagcgcttga cgcggacgtc gatcctgccg cgctgcatgc cgtcgtgcag gatcttcatc
     5941 gtgagctcgt cgtgccagcc gtagtcgaag ggcttcttgg agcgcacggt gtacttcatg
     6001 gcgagctcga gctcgtcctt gcagggctcg cagatgtcga tatccgcgat atggtagtag
     6061 ttcctcgtcg gagtgggatt ctctatgggc ttcttgcaga catcgcagta gagaactttc
     6121 atgcggggct ccttcgtgtt tgtcgctctc tgcgatttat gataccctat gtcgagagcc
     6181 tgcgcaagac ttttatcgga gtggccatgc acaacgactt tcgcgtcgcc ggaaccgcct
     6241 acggaccccg cagccccctc gtcgtggccg agatcggcac ctcccatggc ggcgacctcg
     6301 ccaaggcctt cgagctcgtc gacgcggccg ccgaggccgg cgcggattgc gcgaaattcc
     6361 agtgcgtctt cgccgaggag atcgtccacc ccaacacggg cgtcgtctcc ctgccgggcg
     6421 ggcccacgcc gatcttcgag cgcttccgcg acctggagct gggcgcggac ttctacgcgg
     6481 cgatgaagga gagggccgag gcgaggggca tgctcttcct ctgcaccccc ttcggcctca
     6541 agagcgcgcg gctcctccgg cgcctgggcg tctcggcgat gaaggtggcc agccccgagc
     6601 tcaaccacct gcccctcatc gacgagctcg cctcctacgg cctccccgtc ctcgtctcct
     6661 cgggcgtctc caccctcgcg gacatcgagc gggccctcag gcgcctggcc tgccccgtcc
     6721 tcctcctcca ttgcgtcacc gcctatccgg ccccggcgga ggaatacaac ctccgcgtgc
     6781 tcgccggcct ctcctcggtc ttcggccttc cgacgggcgt ctccgaccac tcggccgacc
     6841 ccgtcctcgt cccctccctt gccgtcgcct cgggcgcctc ctgcgtggag aagcacatct
     6901 gcctctcccg ggtcgacaag ggcctcgacg acccgatcgc cctccctccc gaggacttcg
     6961 cgaggatggc cagggccgtg agggaggcgg ccgagctgcc cgcccaggcg acgatagccg
     7021 ccctctccga gcgcttcggc gccgacgtcg tggagaaggt cttgggcgac ggggtcaagc
     7081 gcctggcgcc gagcgaggcg ggcaactaca gccgcaccaa ccgctccatc cacgccctgc
     7141 gggcgataaa gaagggcgag gtgtactccg cggagaacct cgccctcctc aggacggaga
     7201 agatcctccg ccccggcctg ggccccgagc tcctgcccgc ggtcctgggc cggaaggcgc
     7261 gccgcgccgt gccctcggga gagggcataa cctgggagga cgtcggcgac ataggctagc
     7321 ggcgtgcccg cggcccagcc gggcggcggg cgcgcaaccg cgggcggcga gggcggccgt
     7381 ccggccggcg gtccctactt gaggcggacg accgtcttcc cgtagccgcc ctcttccgga
     7441 cgggcgaagt ggtactcggc gacggcgggc tgcctcctca ggtagtcgtg gatgcccttc
     7501 cccaggatgc cctctcccgt gccgtggatg atggagaaga gggagagccc ctggaggctc
     7561 gccgcgtcta tctgcttctc cacggcctcc agggcctcgg ccaggcggaa gccccgcaag
     7621 tcgagctcga aggccgccgt cgtccttccg ccctcgcccc tgggggcgag ctccaccgcc
     7681 acctgcatct tcgccgcggc ggcctcgggg acgagctcga gcttggactc tggcaccgtg
     7741 aggcggatcg actcggtctc gacgagccag tagcccttct tcgcccttcg caggaccacg
     7801 ccccgcttct tcccgggatt cacgaggacg gccgcgccct cctcgaggac gaggggagcc
     7861 ggggcccgcg ccccgcccgg ggcgccggcc ttgcgcttcc ccggctccgg gccgcccgcg
     7921 gcctcggacg cggcccgcgc cgccctgtcg gccagctccg cgccgcgggc ctcctcggcc
     7981 gcctcctccc tcgccgactc gaggcggcgc tcctcgccct cgacggcctc cgcgaagccc
     8041 gcgaggaagt ccttcacggc cctcgtcttg tccgagccga ggctgccctc gccctcgcgg
     8101 atctccttca cgaggttctc cagggtcttg cggctctccc ccaggaggcg cctgatgtcg
     8161 gagacgccct gctccctgag ctcgagctcc cgctgcctga gcttgagccg cgagaggtcg
     8221 gccttgcgct gctcctcgcg ggcctccttg agcctggagc gccgctccga ctccaggtcc
     8281 tcgagcttcc ggtgcttctc ggtgaggccc ttgatgagct cggagacgtc ggcccgctcc
     8341 tccgcgaggt acttgcgggc cccctcgatg atctccgacc tgaggccgtg ctgggaggcg
     8401 acctccaggg cgcggctctc gccgggcacg cccatgagga cgcggtaggt cggctggagg
     8461 ctcgcggcgt cgaagtccat ggaggcgttg aggcagccgg gcctcgtgta gccgtagttc
     8521 ttgaggatgc cgtggtgggt cgtgaggacg gtgaggcagc cgcggccgat gaaccagtcc
     8581 aggaggccca tcgcgaccgc gcagccctcc tcggggtcgg tgccggagcc gagctcgtcc
     8641 aggaggacga ggctccgcgg gccggcgtcg ttcgctatgc cgcctatcac gcgcatgtgg
     8701 cccgagaagg tggagaggga ctggtcgatg gactgctcgt cgccgatgtc cgcgtagacc
     8761 gcgtcgaaga ccgcgaagcc cgactcgggc gcggcgggca gggccaggcc gaactggttc
     8821 atgagggcga ggaggccgac ggtcttcagg gtgaccgtct tgccgcccgt gttcggcccc
     8881 gtcacgatga gggtcctcgt cccggcgggg agctctacgt cgatgggcac ggccctgcgg
     8941 ccgatgaggg ggtgcctggc cttccttatc gtgaagccct cggccaggtc gcgggccagg
     9001 acgaggccgc ccagcctcgc gtggcgggcc ctcgcgtaga tcccgtcgaa gccgcccatg
     9061 gcccgccgcg cggcggccag ggccggcgcc tcggggagga ggcgctccgt cgtctccctg
     9121 aggatgcgct ggagctcctg gcggtagcgg gcctcctcct ccacgagctc gttgttcttc
     9181 tgcacgaggt cctcgggctc gacgaagacc gtctggccgg tcgccgagac ctcgtggacg
     9241 atgcccttca cgcggccgcg gaagttggcg cggatcgcga ggacggtgcg gccgtcgcgc
     9301 tgggtcggcg cgtcggactg gagcatcgcc cggacctcgg ggttcttgaa gtaggagtcc
     9361 gtgatcgagg cgatgtcgcg gtgggcccgc gcgatgcggt cccgcgcctg gcggagctcc
     9421 gggaggtcgc gcagggagcc gtccttgttg acgaccttga agacgatcct cgaaagcggc
     9481 gcgaggtcgg gggcgccgta gaggaggccg tcgaggagga ggccctccgc ctcgctccat
     9541 cgggcctcgc gctgcgcctc ggccccgggg gcctccgccg gtccggccac ggcctgggcg
     9601 gcgccggagg aggaggcgcc ggtcgcggcc ggggcggcgg cgccggctgc ggggccgggg
     9661 gctgcggcgc cggcatcggc ctgggcggcc gcggggcggg gcgccgactg ggccttgagc
     9721 ctggccgcgc gcagggcctc cagggcctcg c
//
LOCUS       DAOOSJ010000079         9705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4539_length_9705_cov_5.721658, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000079 DAOOSJ010000000
VERSION     DAOOSJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_9705_cov_5.721658"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="guaA"
                     /locus_tag="PLG14_05445"
     CDS             complement(<1..1196)
                     /gene="guaA"
                     /locus_tag="PLG14_05445"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOX48613.1"
                     /translation="MDKILILDFGSQYTQLIGRRVRELGVYAEILPGEAPLSPASLAG
                     AKGIVLSGSPFSVYEPGAPVPHPSAFEAGLPVLGLCYGAQRMTQDSGGRVERLPEREY
                     GRKPVRLLPAGAADPLFAGTAPEFASWMSHGDSIAEPAPGFEVLAASEGGIPAAIRHA
                     KRRLWGLQFHPEVSHCEGGLRILENFVAGVCGAEHGWSMEAYLDEVRASVRAKVGEGK
                     VLLLISGGVDSTVAGALLLKILDPERVHLLYIDTGLMRKAESEEVMRNLRALGATHLH
                     MAEAEEEFLSALAGVEEPEKKREIIGDLFIRIQEREVARLGIPDAYLVQGTLYTDLIE
                     SGKGVGKKAQVIKSHHNVRSPLVEAKRAAGKVIEPLDRLYKDEVRALGRLLGVAEGVV
                     ARHPFPG"
     gene            complement(1181..2410)
                     /locus_tag="PLG14_05450"
     CDS             complement(1181..2410)
                     /locus_tag="PLG14_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOX48614.1"
                     /translation="MNIVVMGAQWGDEGKGKIVDYLAADAGIIVRFSGGANAGHTIVI
                     GGDRYALHLVPSGILYPDKTVILGSGMVIDPEALFSELAGLEAKGIDWKGRVLVSDRA
                     HVVLPSYRRLDKEMEARRRNPIGTTGRGIGVAYAMKASRDGVRVADLSDPSRLGNLEP
                     EDLAFVEAWRGRLEPMAVDLVAHMRAHRNAYALFEGAQGALLDIDTGSYPFVSSGMSC
                     AAGAAAQGGVGPRRVDRVLGVFKAYTTRVGNGPFPTEFTDNSPGSLQQYIRDTGNEYG
                     VTTGRPRRCGYLDLVALRYACAANSIDRLVLTHLDVYDELAEFEACVAYRVRGRIVED
                     FPASVRDLEDAEPVLRTFQGWKRSIAGTRAYDELPREARDYVAFIEEFVETPVSMVSV
                     GSDRSQTIVRESPWTRS"
     gene            complement(2513..3952)
                     /gene="guaB"
                     /locus_tag="PLG14_05455"
     CDS             complement(2513..3952)
                     /gene="guaB"
                     /locus_tag="PLG14_05455"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOX48615.1"
                     /translation="MFEETLSYDDVLLVPGWSEILPRETDVSTRVAGGIRLNVPILSA
                     AMDTVTEERLAVALALQGGAGVIHRNLAPELQAEQVAGVKRYLNWIIDSPVTVEEDRT
                     LREVRAIMARDKVTGLPVVGAEGRLSGIITARDLRFRRDDGALVRDAMTRDPVVEGLD
                     ATPASARAKFDEHRIEKLPVVDGEGRLRGLITVKDMEKHERYPNAATDKAGRLVVGAA
                     VSPQDYEVRVPLLLASRVDFVVLDTAHGDSRNVVEAVRGLKRRWPELPVVGGNVATRE
                     GTRRLIEAGSDAVKVGVGPGSICTTRIVAGVGVPQFTAVLWCAEEAAKSGVPVIADGG
                     IKYSGDIVKAIAAGASAVMLGNLFAGLKEAPGREVLYEGRIYKEYRGMGSMGALKDGA
                     GDRYGIAKDESPVPEGIEGRLPYKGELKAYLHQLVAGLRKGMGYCGCADLEALRKHRN
                     FVRITSAGLRESHAHDVTVTQEAPNYSRQ"
     gene            complement(4076..5503)
                     /locus_tag="PLG14_05460"
     CDS             complement(4076..5503)
                     /locus_tag="PLG14_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOX48616.1"
                     /translation="MEPRDIFDNLSPLDHRYWLANKDVFDALSARLSEKAQVRYAALA
                     EAALLETHLELRGRSDPGLSKAIAEAAAGIDPAEVAAEEEKTRHNVRALVNVLQRKLP
                     EDVAPLVHLGATSVDILDTARALSVRAAVREVLLPLLARVELELCRLCVDEAETPQVG
                     RTHGQHAVPLTFGFAMAEYASRLGKCVLRADELAGALVGKLAGAVGSYNALSMSYKDP
                     EAVERAFLGRLGLEPSEHSTQLAEAEPMLRLLLELNCAFGVVANLADDLRNLQRTEIG
                     EVRESFGEGQVGSSTMPQKRNPWNSEHVKSLWKAFCPRVQTFFMDQISEHQRDLTNSA
                     SERFVAEYLAGLAAAANRMLSVLRGLRVDRERMAANLSMTGGSVLAEPAYILLAESGL
                     ADAHERLRRVTLAAEAEGLAFHEALSREPGTEALLTERLLALGLPPAAEFFAEPAAYR
                     GRAAEKARAIGGKYRALMGRFAESK"
     gene            5630..6976
                     /gene="lpdA"
                     /locus_tag="PLG14_05465"
     CDS             5630..6976
                     /gene="lpdA"
                     /locus_tag="PLG14_05465"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOX48617.1"
                     /translation="MYDIIIIGGGPGGYVAAERAGHAGLKVLLVEKAELGGTCLNRGC
                     IPTKSLLAGAKLYKHAKEGARYGLKAEGLSFDFAAAAAWKDDVVGKLRSGVEFSVKKS
                     GAEVYSGSARLAAGRRVVLDDGRELEAKDVILATGSSPALPPIPGLADNPKVVTSDGL
                     LALAAPPERLVVVGGGVIGVEFASFFSAIGTKVTVVEMLPEILPFMDAELVGVFRRTL
                     RGLAVETGAKVERIEGGTVHYEKGGEKRSAEGDLVLVATGRRPNVQGMGLEEAGVAFS
                     PKGIEVDDCLRTGVPGVWAVGDATGRALLAHAASAMGECAVEGILGRPRPFAFKAVPW
                     VVYGDPECAGVGMTEAEAKAAGLDFAKVLLPARVSGRFLAENGQAAQGAVKLLAERGS
                     GRLLGAGIVAPYAGEMVWGLQPLLARGATVAELKETIFPHPTVSELIREAAWELEV"
     gene            complement(7141..7653)
                     /gene="ybaK"
                     /locus_tag="PLG14_05470"
     CDS             complement(7141..7653)
                     /gene="ybaK"
                     /locus_tag="PLG14_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOX48618.1"
                     /translation="MAKAGQERQKTNALRILDGLGIAYRTIAYEVDEEDLSAESVAAK
                     VSLDPERVFKTLALRGASGAVFLACVPAGSELDLKKASKAAGEKSVDMLPLKELQPTT
                     GYVRGGCSPIGAKRKFPVYLDETATLFEEISVSAGQRGLQVLLAPEELRRAASEQPGG
                     QAEAGFADLV"
     gene            complement(7675..8409)
                     /locus_tag="PLG14_05475"
     CDS             complement(7675..8409)
                     /locus_tag="PLG14_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOX48619.1"
                     /translation="MGRSRPYRLAAATRENGLTTTLRLEPLKGSVPRYLPGQHLYLRF
                     VGTTLPAEEHPFTISSSPSLPGALTVTVKAAGTYTNRLGELEPGARALVDAPHGGFSY
                     LKWPDPRRHVFIAGGVGATPFLSMLNWMADRDSDRDLLFVWAVLSRDQLFGLADLKSF
                     GKTFPRLKTVVVAEGDPLWTGEKGRVDRALLERLVPAYFGGAAFDWNSAVYAVSGPPP
                     MRKAVLSALRGLGVAGRNLRVGRFAR"
     gene            complement(8490..>9705)
                     /locus_tag="PLG14_05480"
     CDS             complement(8490..>9705)
                     /locus_tag="PLG14_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOX48620.1"
                     /translation="YSRDVLGAFDYFYKKGRLEIITSAATHCFLPSYRENQEAVTAQI
                     ETALVSHRRAFGKHPGGFWLPHLGWYPGLDELLRSYNIQYSVVTTKGALLGEPTPRSG
                     SFAPVQSPAGLTLFIRDAAATEAVWSESSGYPADPLYRDFYRDIGFDLPLDYVGPYVE
                     EGPTRTFTGFKYWAVTGRTDQKRPYDAAAARARADEHAAAFLADRVAAAKPVAALIDK
                     PALMVCPYDAELFGHWWFEGPAWLESVFRRAAREPELRLATLGEYHRLYPEAQVSMPE
                     FSSWGDGGYAEVWIDGANDWIYRHAFKATERMSELAERFPDESGLRERILNQAAREVL
                     LMMGSDWAFLMRAGKSSAFARRQIEDAVTNFNRIYEMLSSNTVGTEWITRLEKRDNLF
                     PDINYRIFRKKR"
BASE COUNT         1336 a         3269 c         3704 g         1396 t
ORIGIN      
        1 ccggggaagg ggtggcgggc gacgacgccc tccgccacgc ccaggagccg gcccagggcc
       61 cgcacctcgt ccttgtagag ccggtccagg ggctcgatca ccttgccggc cgcgcgcttc
      121 gcctccacga gggggctccg cacgttgtgg tggctcttga tgacctgcgc cttcttgccg
      181 acgcccttgc ccgactcgat gaggtcggtg tagagggtgc cctgcacgag gtaggcgtcg
      241 gggatgccca ggcgcgcgac ctcgcgctcc tggatcctga tgaagaggtc gccgatgatc
      301 tcccgcttct tctcgggctc ctcgacgccg gccagggcgg agaggaactc ctcctcggcc
      361 tccgccatgt ggagatgggt cgcgcccagg gcgcggaggt tccgcatgac ctcctcgctc
      421 tccgccttgc gcatgaggcc cgtgtcgatg tagaggaggt ggacgcgctc cgggtccagg
      481 atcttgagga ggagggcgcc cgcgacggtc gagtcgacgc cgccggagat gaggaggagg
      541 accttgccct cccccacctt ggcccggacg gaggccctga cctcgtccag gtaggcctcc
      601 atgctccagc cgtgctccgc gccgcagacg cccgcgacga agttctcgag gatcctgagg
      661 ccgccctcgc agtggctcac ctcggggtgg aactggaggc cccagaggcg gcgtttggcg
      721 tggcggatcg ccgcgggaat gccgccctcg gaggccgcga ggacctcgaa gccgggcgcg
      781 ggctcggcga tcgagtcgcc gtggctcatc cagctcgcga actccggcgc cgtcccggcg
      841 aagagggggt cggccgcgcc cgcggggagg aggcgcacgg gcttccgccc gtactcgcgc
      901 tcgggcaggc gctccacgcg gccgcccgag tcctgggtca tgcgctgggc gccgtagcag
      961 aggcccagga cgggaaggcc cgcctcgaag gcggaaggat gcgggacggg cgcccccggc
     1021 tcgtagacgg agaagggcga gcccgagagg acgattccct tcgcgcccgc gagggaggcc
     1081 ggggagagcg gcgcctcgcc cgggaggatc tcggcgtaga cgccgagctc gcgcacgcgg
     1141 cggccgatga gctgggtgta ctggctgccg aagtcgagga tcaggatctt gtccacgggc
     1201 tctccctcac gatggtctgg ctgcggtcgg agccgacgga gaccatggag acgggggtct
     1261 cgacgaactc ctcgatgaag gcgacgtagt cccgggcctc gcggggcagc tcgtcgtagg
     1321 cccgcgtgcc cgcgatgctc cgcttccagc cctggaaggt gcgcaggacg ggctccgcgt
     1381 cctcgaggtc cctcaccgag gcggggaagt cctccacgat gcggccgcgc acgcggtagg
     1441 cgacgcaggc ctcgaactcg gcgagctcgt cgtagacgtc caggtgggtg aggaccaggc
     1501 ggtcgatgga gttggccgcg caggcgtagc gcagggccac caggtccagg tagccgcagc
     1561 ggcggggccg gcccgtcgtg acgccgtact cgttgcccgt gtcgcggatg tactgctgga
     1621 gggaacccgg cgagttgtcg gtgaactcgg tcgggaaggg gccgttgccg acgcgggtcg
     1681 tgtaggcctt gaagacgccg aggacgcggt ccacgcggcg ggggccgacg ccgccctggg
     1741 cggccgcgcc cgcggcgcag gacatgccgc tggagacgaa ggggtagctg cccgtgtcga
     1801 tgtccaggag cgctccctgg gccccctcga agagggcgta ggcgttgcgg tgggcgcgca
     1861 tgtgggcgac gaggtcgacc gccatgggct cgaggcggcc gcgccaggcc tccacgaagg
     1921 cgaggtcctc aggctccagg ttcccgaggc gcgaggggtc cgagaggtcg gccacgcgga
     1981 cgccgtccct ggaggccttc atcgcgtagg cgacgccgat gccgcggccc gtggtgccga
     2041 tggggttccg gcgcctggcc tccatctcct tgtcgaggcg gcggtagctc gggaggacga
     2101 cgtgggcccg gtcggagacg agcacgcggc ccttccagtc gatgcccttg gcctccaggc
     2161 ccgcgagctc ggagaagagc gcctccgggt cgatcaccat gcccgagccg aggatgacgg
     2221 tcttgtccgg gtagaggatg cccgagggca caaggtggag ggcgtagcgg tcgcccccga
     2281 tgacgatggt gtggccagcg ttggcccctc cggagaagcg gacgatgatg cccgcgtccg
     2341 cggccaggta gtcgacgatc ttgcccttgc cctcgtcgcc ccactgggcg cccatgacga
     2401 cgatgttcat gctgttggcc tctctttggt ctcaagtaag ctcgacggag gccctcccgc
     2461 ggaccccgcc cgtcccgtcg cggtcaagta tagcggaagc ggcccctgca ggtcactgcc
     2521 gggagtaatt gggcgcctcc tgggtgacgg tgacgtcgtg ggcgtggctc tccctgaggc
     2581 ccgccgaggt gatgcggacg aaattccggt gcttccgcag ggcctcgagg tcggcgcagc
     2641 cgcagtagcc catgcccttg cggaggccgg cgaccagctg gtggaggtag gccttgagct
     2701 cgcccttgta ggggaggcgt ccctcgatgc cctcgggcac cggcgactcg tccttagcga
     2761 tcccgtagcg gtcgcccgcc ccgtccttga gcgcccccat ggagcccatg ccccggtatt
     2821 ccttgtagat gcggccctcg tagaggacct cgcggcccgg ggcctccttg aggccggcga
     2881 agaggttgcc gagcatcacc gccgaggcgc cggcggcgat cgccttcacg atgtccccgg
     2941 agtacttgat gccgccgtcc gcgatcacgg ggacgccgga cttcgcggcc tcctccgcgc
     3001 accagagcac ggcggtgaac tggggcacgc cgacgcccgc gacgatgcgg gtcgtgcaga
     3061 tggagccggg gcccacgccg accttgaccg cgtcggagcc cgcctcgatg agccgcctcg
     3121 tgccctcgcg ggtcgcgacg ttgccgccga cgacgggcag ctcgggccag cgcctcttga
     3181 ggccgcgcac cgcctccacg acgttgcgcg agtccccgtg ggccgtgtcg aggacgacga
     3241 agtccacgcg ggaggcgagg aggaggggca cgcggacctc gtagtcctgg ggggagacgg
     3301 cggcgccgac gacgaggcgc ccggccttgt cggtcgcggc gttcgggtag cgctcgtgct
     3361 tctccatgtc cttcacggtg atgaggcccc ggagccggcc ctcgccgtcc acgacgggga
     3421 gcttctcgat gcggtgctcg tcgaacttgg ccctggcgct cgcgggcgtg gcgtccaggc
     3481 cctccacgac tgggtcgcgg gtcatcgcgt cccgcacgag ggccccgtcg tcacggcgga
     3541 agcgcaggtc gcgcgcggtg atgatgcccg agaggcgccc ctccgcgccg acgacgggga
     3601 ggcccgtcac cttgtcgcgg gccatgatgg cccgcacctc gcggagggtc ctgtcctcct
     3661 ccaccgtcac ggggctgtcg atgatccagt tgaggtagcg cttgacgccc gcgacctgct
     3721 cggcctggag ctcgggcgcc aggttccggt ggatgacgcc ggcgccgccc tggagggcca
     3781 gggccaccgc gagcctctcc tccgtgaccg tgtccatcgc cgccgagagt atcgggacgt
     3841 tgaggcggat gcctcccgcc acgcgggtgg agacgtcggt ctcccgcggg aggatctccg
     3901 accagccggg cacgaggagg acgtcgtcgt aggaaagcgt ttcttcgaac atcggtttct
     3961 ccgggcggct cccgggtgcc ccctttcggg ggcgggcccg ggccgcgggc tatcgcgatg
     4021 tccgccggcc gcccgggccg gcccggtgcc ggcgctcagg cggccggacg gacccttact
     4081 tggactcggc gaagcgcccc atgagggcgc ggtacttccc gcctatcgcg cgggccttct
     4141 cggcggcccg cccccggtag gccgcgggct ccgcgaagaa ctccgccgcg gggggaaggc
     4201 ccagggcgag gaggcgctcc gtgaggaggg cctccgtccc cggctcgcgg gagagggctt
     4261 cgtggaaggc gagcccctcc gcctccgcgg cgagggtcac gcgccggagg cgctcgtggg
     4321 cgtcggcgag gccggactcc gcgaggagga tgtaggcggg ctcggcgagg acggagccgc
     4381 ccgtcatgga gaggttggcc gccatccgct cccggtccac gcggaggccg cggaggacgg
     4441 agagcatgcg gttcgcggcg gcggcgaggc ccgcgaggta ctcggcgacg aagcgctcgc
     4501 tcgcggagtt cgtgaggtcg cgctggtgct cggagatctg gtccatgaag aaggtctgga
     4561 cccgggggca gaaggccttc cagaggctct tcacgtgctc ggagttccag gggttccgct
     4621 tctgcggcat cgtggaggag ccgacctgcc cctcgccgaa ggattcgcgg acctcgccga
     4681 tctccgtgcg ctggaggttc cgcaggtcgt ccgcgaggtt cgcgacgacg ccgaaggcgc
     4741 agttgagctc gagaaggagg cgcagcatgg gctcggcctc agcgagctgg gtcgagtgct
     4801 cggagggctc gaggccgagc cggcccagga aggcgcgctc gaccgcctcg gggtccttgt
     4861 agctcatgga gagggcgttg taggagccga cggcgccggc gagcttcccg accagggccc
     4921 ccgcgagctc gtccgcgcgg aggacgcact tcccgaggcg gctcgcgtac tcggccatcg
     4981 cgaagccgaa ggtgaggggg acggcgtgct ggccgtgggt tcggccgacc tggggcgtct
     5041 cggcctcgtc gacgcagagg cggcagagct cgagctcgac tctcgcgagg agggggagga
     5101 ggacctcgcg caccgccgcg cggacggaga gggcccgcgc cgtgtcgagg atgtcgacgc
     5161 tcgtcgcgcc caggtgcacg aggggcgcga cgtcctcagg cagcttgcgc tgcaggacgt
     5221 tgacgagggc gcggacgttg tgcctggtct tctcctcctc cgcggcgacc tccgcggggt
     5281 cgatgcccgc ggccgcctcg gcgatggcct tggagaggcc ggggtccgag cggcccctga
     5341 gctcgagatg ggtctcgagg agggcggctt ccgccagggc ggcgtagcgg acctgggcct
     5401 tttcggagag ccgcgccgag agggcgtcga agacgtcctt gttggccagc cagtagcgat
     5461 ggtccagggg ggagaggttg tcgaagatgt cgcggggctc catgtcccat gttgccacgg
     5521 gccgcgggcg gagatcaagc gggcccccgg gggcaaacca gtttaggccc ctcgatattt
     5581 ccgctttatt gatttgacgg gaaagcggag cccgggctac cctgtcgcca tgtacgacat
     5641 catcatcatc ggcggcgggc ccggcggcta cgtggcggcg gagcgcgccg gccacgcggg
     5701 cctcaaggtc ctcctcgtag aaaaggccga actcggcggc acctgcctga accgcggctg
     5761 catccccacg aagagcctcc tcgcgggggc caagctctac aagcacgcga aggagggggc
     5821 caggtacggc ctcaaggccg aggggctctc cttcgatttc gccgccgcgg cggcctggaa
     5881 ggacgacgtc gtagggaagc tccgctccgg cgtggaattc tccgtgaaga aatccggcgc
     5941 ggaggtctac tcgggctcgg cccgcctcgc ggccggaagg cgcgtcgtcc tggacgacgg
     6001 cagggagctc gaggcgaagg acgtcatcct cgcgaccggc tcctcccccg ccctcccgcc
     6061 catccccggc ctcgcggaca atccgaaggt ggtcacgagc gacggcctcc tcgccctcgc
     6121 cgcgccgccc gagcgcctcg tcgtcgtggg cggcggcgtc atcggcgtgg agttcgcctc
     6181 cttcttctcc gccatcggca cgaaggtgac cgtcgtcgag atgctcccgg agatcctccc
     6241 cttcatggac gccgagctcg tcggcgtctt caggaggacc ctccgcggcc tggccgtgga
     6301 gacgggggcg aaggtggagc gcatcgaggg cgggaccgtc cactacgaga agggcggcga
     6361 gaagcggagc gccgagggcg acctggtcct ggtcgcgacg ggccggaggc ccaacgtcca
     6421 gggcatgggc ctcgaggagg ccggcgtcgc cttctccccc aagggcatcg aagtcgacga
     6481 ctgcctccgg acgggcgtac cgggcgtctg ggccgtcggc gacgcgaccg gccgcgccct
     6541 cctggcccac gccgccagcg cgatgggcga atgcgccgtg gaggggatcc tcggccggcc
     6601 ccggcccttc gccttcaagg ccgtgccctg ggtcgtctac ggcgacccag agtgcgccgg
     6661 cgtcggcatg acggaggcgg aggcgaaggc cgccggcctc gacttcgcca aggtcctcct
     6721 ccccgcgcgg gtctcgggcc gcttcctggc cgagaacggc caggcagccc agggcgccgt
     6781 gaagctcctc gcggagcggg gctcggggag gctcttgggc gcgggcatcg tcgcccccta
     6841 cgctggcgag atggtctggg gcctccagcc cctcctggcc cggggcgcga cggtcgctga
     6901 gctcaaggag acgatctttc ctcacccgac cgtgtcggag ttgatcaggg aagcggcctg
     6961 ggagctggaa gtgtaacctg cgggacgggg gctgatcccc cgtcccccct cgcggcgctc
     7021 cgagtctcgc gccgcgtccc gccgcgcgcg gaccgccgcg agcggaccgt cgcgctcctg
     7081 accgcatggg ccgccgcgcg cagactgccg cgccgcgggt tgccgcgcgc gggccgcagg
     7141 ctagaccagg tcggcgaagc ccgcctcggc ctggccgccc ggctgctcgc tggcggcgcg
     7201 gcggagctcc tcgggcgcga ggaggacctg gaggccgcgc tggccggcgc tcacggagat
     7261 ctcctcgaac agcgtcgccg tctcgtcgag gtagacgggg aacttccgct tcgcgccgat
     7321 gggagagcaa ccgccgcgga cgtagcccgt cgtcggctgg agttccttca ggggcagcat
     7381 gtcgacgctc ttctccccgg cggccttcga ggccttcttg aggtcgagct ccgagcccgc
     7441 cggcacgcag gccaggaaga ccgcgccgga ggccccgcgg agcgccaggg tcttgaagac
     7501 ccgctcggga tcgaggctga ccttggccgc gacggactcg gcggagaggt cttcctcgtc
     7561 cacctcgtag gcgatcgtgc ggtaggctat cccgagcccg tcgaggatgc gcagcgcgtt
     7621 ggtcttctgg cgctcctgcc ctgccttggc catggccgct ccttggaacg gggcctagcg
     7681 cgcgaagcgc ccgaccctga ggttgcgccc cgcgaccccg aggccgcgca gggctgagag
     7741 gacggccttc cgcatcggcg gagggcccga gaccgcgtag accgcggagt tccagtcgaa
     7801 ggccgcgccg ccgaagtagg cgggaacgag gcgctcgagg agggcgcggt ctaccctgcc
     7861 cttctcgccc gtccagagcg gatccccctc ggccacgacg acggtcttga ggcgggggaa
     7921 agtcttcccg aaggacttga ggtcggcgag gccgaagagc tggtcgcgcg agaggacggc
     7981 ccagacgaag aggaggtcgc ggtccgagtc ccggtcggcc atccagttca gcatcgagag
     8041 gaagggggtg gccccgacgc cgcccgcgat gaagacgtgg cggcgggggt cgggccactt
     8101 caagtaggaa aaaccgccgt ggggcgcgtc gacgagggcc cgcgcccccg gctcgagctc
     8161 gccgagccgg ttcgtatagg tcccggcggc cttgaccgtc acggtcaggg ctccgggaag
     8221 gctcgggctc gaggagatcg tgaagggatg ctcctcggcg ggaagggtgg tcccgacgaa
     8281 gcggaggtag aggtgctggc ccggcaggta gcgggggacg gagcctttca ggggctcgag
     8341 ccgcaaggtc gtcgtgaggc cgttctccct cgtcgccgcc gcgagcctgt agggacggct
     8401 cctgcccaag aggggcccaa ggaggggcct gaggagacga tgccaaagga ggccgaaggg
     8461 agggaccatg ccgccgcgcc ggggaggccc tagcgcttct tccggaagat ccggtagttg
     8521 atgtcgggga agaggttgtc ccgcttctcc agccgcgtga tccactccgt acccaccgtg
     8581 ttggaggaga gcatctcgta gatgcggttg aagttggtga ccgcgtcctc gatctgccgc
     8641 cgggcgaagg cgctcgactt gcccgcgcgc atgaggaagg cccagtccga gcccatcatg
     8701 aggaggacct cgcgggcggc ctggttcagg atgcgctcgc ggaggcccga ctcgtcgggg
     8761 aagcgctcgg cgagctcgga catgcgctcc gtcgccttga aggcgtggcg gtagatccag
     8821 tcgttggccc cgtcgatcca gacctccgcg tagccgccgt cgccccagga cgagaactcc
     8881 ggcatggaga cctgggcctc ggggtagagg cggtggtact cgcccagggt cgcgagcctg
     8941 agctcgggct ccctggcggc gcggcggaag acggactcga gccaggccgg cccctcgaac
     9001 caccagtggc cgaagagctc ggcgtcgtag gggcagacca tgagggcggg cttgtcgatg
     9061 agggcggcca cgggcttggc cgcggccacg cggtcggcga ggaaggcggc cgcgtgctcg
     9121 tcggcccgcg cgcgggcggc ggccgcgtcg tagggccgct tctggtcggt ccggcccgtg
     9181 acggcccagt acttgaagcc ggtgaaggtg cgcgtcgggc cctcctccac gtaggggccg
     9241 acgtagtcga gggggaggtc gaagccgatg tcccggtaga agtcgcggta gagggggtcg
     9301 gcgggatagc ccgagctctc cgaccagacg gcctcggtcg cggcggcgtc cctgatgaag
     9361 agggtgaggc cggccgggct ctggaccggc gcgaaggagc ccgagcgggg cgtcggctcg
     9421 ccgaggaggg cgcccttcgt cgtcaccacg ctgtactgga tgttgtagga gcgcaggagc
     9481 tcgtccaggc ccggatacca gcccaggtgg ggcagccaga agcctcccgg atgcttgccg
     9541 aaggccctgc ggtgggagac gagggccgtc tcgatctgcg ccgtcaccgc ctcctggttc
     9601 tcgcggtagc tcgggaggaa gcagtgggtc gcggccgagg tgatgatctc caggcggccc
     9661 ttcttgtaga agtagtcgaa ggcgcccagc acgtcgcgcg agtag
//
LOCUS       DAOOSJ010000080         9575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4600_length_9575_cov_6.011870, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000080 DAOOSJ010000000
VERSION     DAOOSJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4600_length_9575_cov_6.011870"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLG14_05485"
     CDS             complement(<1..201)
                     /locus_tag="PLG14_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48621.1"
                     /translation="MAAKAKRARQTSRGKSRPGTRRHFAATSLGLVVAALAATLLLTM
                     LTTDSGASERRRAAAGNGAARGV"
     gene            complement(337..1176)
                     /locus_tag="PLG14_05490"
     CDS             complement(337..1176)
                     /locus_tag="PLG14_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOX48622.1"
                     /translation="MNNTTAADSKEDVLRSYYAAIKRVALLTADEERELSRQIAAGDE
                     AARRRLIEANLRLVVKIARGFVTADMPLVDLIQEGNLGLIKAAEKFDGERNVRFSTYA
                     SWWIKQSITRSLVNSRRAIRLPHRKEELLKRIQKTYNSLTQVFQREPSAAEIAAELAI
                     PERSVEAVMGMSTALLSLDTESGEDSGAVIDVVEDYTYSPDNAFMRDCARDEAMRMLE
                     ILLEKERRILLYRFEFFGGEKYTLKRIGTELGISPETVRQIEKRALKKLRDNAPESVA
                     VGM"
     gene            complement(1546..1728)
                     /locus_tag="PLG14_05495"
     CDS             complement(1546..1728)
                     /locus_tag="PLG14_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48623.1"
                     /translation="MKTMPEGYRTEVPVSEDILRKFVVALVAVLALFTFIRDYPGSSP
                     QQGAGRQVEARAELIP"
     gene            complement(1744..3090)
                     /locus_tag="PLG14_05500"
     CDS             complement(1744..3090)
                     /locus_tag="PLG14_05500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX48624.1"
                     /translation="MESIITGQKIAKRRPFKPLAREASLSRPGQASVFARREGTKVRK
                     ADFFLLGEAGKPAGGAFRASGGLRPPLGLRLPKAFRLPKAFRISRDFRRPDASRAPEV
                     LRGEESPRRRSVLSRPPKAEREDQPSRGRGREARLRRWLVAAGSGLGLFALASALFLV
                     FAPPAFPRPEGAFLYDDQGAEALLFAYASPELAEASLDEGLEPPPAPKTLELSSYIVK
                     KGDSIGAIAKRFGLSVDTLVGMNGVKSAKGLGVGVELKIPNMDGILYKVAKGDSLGSI
                     AKRYKVGITDLADANDLGTAVLVPGQSVFIPGARLPQAELKRFLGESVIWPVRGAISS
                     YYGYRSDPFSGTRRFHAAIDIVSNTGTPIKAPMDGRVADVGYNSVYGNYIILSHADGM
                     QSLYGHLSSTAVKIGKTVAQGAVIGFVGNTGYSTGPHLHFGLFKRGSPVNPLKMLK"
     gene            3149..3661
                     /gene="lepB"
                     /locus_tag="PLG14_05505"
     CDS             3149..3661
                     /gene="lepB"
                     /locus_tag="PLG14_05505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOX48625.1"
                     /translation="MKRSRPRPGVLILLAVLSAALLKAFVVDLAVVEGRSMLPALEQG
                     DLVLVLRCAYGLRLPGAGGEGAYLLRWGRPSPGEIVAAASPRDGLAVVKRVAAVGPAA
                     LVVEEGRLRGAGLDLPLVAAMAESLGPGLYLGRGSVFLLGDNAPESVDSRDYGAVPVE
                     SIAGRVLFWI"
     gene            3678..5555
                     /locus_tag="PLG14_05510"
     CDS             3678..5555
                     /locus_tag="PLG14_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOX48626.1"
                     /translation="MQKNLTRYWLAVGILAAAALVLLGRYAVLAAEKGPAAARAAAEA
                     ERGAIVDRTGRPLAMDSALYNIAVWRPETGAEAFASDAARLASILGIPEAEILAKRES
                     GESDFFYLKKRVAPQVARAVQDGKASGSFAGVVVEKVSGRIYPEKRLASHLVGFVGDG
                     NRGLAGVESKYEAELSPSPAPAPDASSGLLKGNEVQLAVDSAIQFSLEEIARKAMADT
                     GAESVILLAGDARTGEILAYVAMPDFDPNEYYAAPAEAWSDLPSVYAYEPGSVFKIFS
                     MASVMDLGGIDGSTTFICDGAYRRKTPSGEEIVIKCLGSHGPVNLERILELSCNAGAG
                     YASDTVQSLDLYERLTAFGFGARTGIALAGESPGLLRSPDAWSLRSKPTIAMGQEVLV
                     TAVQMLSAATAVANGGVLLKPIAVRKVVAPDGSLVYENAPQAVRRVVSADTAAAILGA
                     MEAASGEAGTGRRAKVKDVRMAVKTGTAQMADRATRRYSETDFIASTVAIFPAEAPRI
                     VLYLAIVKPKGESYYGGRIAAPVVREAAEAVLALTDLPRGESPTVLHPGAVSLPRLAA
                     PTVGETMPDLRGTPKRLLLPLLGRGDLKVRIEGEGYVTRQRPAPGEAVVPGTEIVLEL
                     E"
     gene            5552..7357
                     /locus_tag="PLG14_05515"
     CDS             5552..7357
                     /locus_tag="PLG14_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOX48627.1"
                     /translation="MSPDGAPAFDPLLLAANLEALAGRFPALARLVGEAGAPDLEPFA
                     TRSGLPSARLAGLALHSAYDPAAEAARLAATLPGSADTVLLLGFGLGYAAEACLAAGG
                     PERAIVCEAEPGLLRAALALRDLRPLLADERLGFVVGGEPEGLLAALEDSGARRAGLL
                     GLKAAEAASPDWYGRARAAVQRHQAKEGINENTLRRFGRLWVRNLAKNLPEIGRLPGV
                     SRLEGRFAGFPALVLAAGPSLDEALPLIGELAERCLVVCVDTALRSLLRAGLEPDFLV
                     VVDPQYWNWRHLEGLSSPGSILVSESAAWPAVFRFGARAAFLGGSLFPLGRAVEAYAS
                     PKGLLGAGGSVATSAWDLARLLGASPIYMAGLDLSFPGGATHAKASLFEQRALSGGRR
                     LSPADSAQAAALCSADPSPAPDNSGGLVRTDRRMALYAWWFESRLARPSSPPTRSLSA
                     RGLAIPGMPLASLDEVLALPPRRSELAERLDAAAALREEAEAPARLQEGRLALLADLA
                     RVEAVAGAAAQAAAAARAALASGGDPRAELALLDRADAELLGNAARDVAGFLLPPLGE
                     LLSRRARDLAESLGQSEAIYRGAAESARYHLQVLA"
     gene            complement(7554..8438)
                     /locus_tag="PLG14_05520"
     CDS             complement(7554..8438)
                     /locus_tag="PLG14_05520"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOX48628.1"
                     /translation="MTRDLEESWSFIPGVARGRRPGEGYSFVQAIEGRTARSWWFEFP
                     LSAFSASRRELSVRVGGCEFSRSGLRLDLSSPEGRFEGELGFGPPTLLPSPLHSPGVM
                     GPYTFVPFMECRHGLVSLHHGLSGSLRHDGRPIDFAGGRGYAEKDWGRSMPESWLWTQ
                     SNNFPVEGDSFMLSVARIPWLGGSFTGFLCAASLGGRLLQEASYTGARLGLLQADEAS
                     ARVVVERGGERIEARIARARGGSLRAPVDGLLSRRIAESVDAVLELEWSKGGKVLFSG
                     RAPKAGLEIVGRVPAVRA"
     gene            8714..9280
                     /locus_tag="PLG14_05525"
     CDS             8714..9280
                     /locus_tag="PLG14_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOX48629.1"
                     /translation="MQSRDEAIRLWREHNKDESLFRHALSVEAAMRHFAAKAGEDPEY
                     WGLVGLLHDIDYEEHPKEHLKHARPILSGAGYPEPFVRAVECHGFGICTDIEPVLGME
                     KVLFAVDELTGFIAACAFVRPSKSVLDLEVKSVKKKWGTAAFAAGVSREVIEKGAAML
                     GLPLDELIAETIQALRGVAAEVGLKGSL"
     gene            9458..>9575
                     /locus_tag="PLG14_05530"
     CDS             9458..>9575
                     /locus_tag="PLG14_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOX48630.1"
                     /translation="MLEKISQKFGDVVRTIAGKSAISEKNVEEAVEQIKLALL"
BASE COUNT         1317 a         3502 c         3306 g         1450 t
ORIGIN      
        1 gacgcctctc gcggcgccgt ttcccgcggc cgcccgccgg cgctccgaag cgcccgagtc
       61 cgttgtgagc atcgtgagga ggagggtggc cgcgagggcg gcgacgacca ggccgaggct
      121 cgtcgcggcg aagtgccgcc tcgtgcccgg tctgcttttc ccgcggctcg tctgtcttgc
      181 tcgtttcgcc ttggctgcca ccggctcgac tatagcctcg agccccctga ggggcaaggc
      241 cggccgcttc ccggcggcgc tccgcggccg cggcgatcct gggcggccgc ggccccgccg
      301 tccggcgtcc cgccggcgcg ggacggcccg aagggcctac atcccgacgg cgacgctctc
      361 cggcgcgttg tccctgagct ttttcagggc gcgcttctcg atctgccgca cggtctcggg
      421 ggagatgccg agctccgtgc cgatgcgctt gagcgtgtac ttctcgccgc cgaagaactc
      481 gaagcggtag aggaggatgc gcctctcctt ctccaggagg atctcgagca tccgcatcgc
      541 ctcgtcgcgg gcgcagtccc tcatgaaggc gttgtcgggg ctgtaggtgt agtcctccac
      601 gacgtcgatc acggcccccg aatcctcgcc gctctcggtg tccagggaga ggagggcggt
      661 cgacatgccc atcacggcct ccacggagcg ctccgggatc gcgagctcgg cggcgatctc
      721 cgcggcggag ggctcgcgct ggaagacctg ggtcaggctg ttgtaggtct tctggatgcg
      781 cttgaggagc tcctccttcc tgtgcggaag gcggatcgcg cggcggctgt tcacgaggga
      841 gcgggtgatg gactgcttga tccaccagga ggcgtaggtc gagaaacgca cgttgcgctc
      901 cccgtcgaat ttctcggcgg ccttgatgag gccgaggttc ccttcctgga tcaggtccac
      961 gaggggcatg tcggcggtga cgaagccccg cgctatcttc acgacgagcc gcaggttggc
     1021 ctcgatgagg cggcgcctgg ctgcctcgtc gcccgccgct atctgcctgg agagctccct
     1081 ctcctcgtcg gcggtcagga gcgccacgcg cttgatcgcc gcgtagtacg agcgaaggac
     1141 gtcctctttc gaatcagctg ccgtagtgtt gttcatatca ttggctccgt ctattgatat
     1201 gcaatatccg tgccaatcaa aaagcagcgt tttcccgcct gcctaaaaca ttgtccggaa
     1261 agacattgct gtcccgtccc tccatggccg tctgtcctgc ctcggggaat agtctacaga
     1321 tgtgcacgtt aacgttgtat aagcgtatga ggaagtatac tggcgccgat agctcgtgag
     1381 ggggtattgg agagagagga ggaatgtgga atccgggcgg gggcggcatc gcggataaaa
     1441 tcgtccacga gacgcgttag ttcacgaata tatctccacg ccgggcggaa ttcaggtcca
     1501 gaccggcggc aaatgaggca aaacgcggaa ttccggcgaa aattcctagg ggatgagttc
     1561 ggcgcgggct tcgacttggc gcccggcccc ctgctggggg ctggagccgg ggtaatcgcg
     1621 gatgaaggtg aagagggcca ggacggcgac gagggcgacc acgaatttac gcaggatatc
     1681 ctccgaaacg ggaacctcgg tacgatagcc ctccggcatc gtcttcatca agcggagcct
     1741 ccgtcatttc agcattttta ggggattcac cggggagccc cgtttgaaga ggccgaaatg
     1801 gaggtgggga cccgtcgagt agcccgtgtt gccgacgaag ccgatcacgg ctccctgggc
     1861 gacggtcttt ccgatcttga cggccgtcga gctcaggtgg ccatagaggg actgcatgcc
     1921 gtcggcgtgg ctcaggatga tgtagttgcc gtagaccgaa ttgtagccga cgtcggccac
     1981 gcggccgtcc atcggcgcct tgatcggcgt cccggtgttg gagacgatgt cgatggcggc
     2041 gtggaagcgg cgggtgcccg agaaggggtc ggagcggtag ccgtagtagg agctgatggc
     2101 gccccgcacc ggccagatga cgctctcgcc caggaagcgc ttgagctcgg cctgggggag
     2161 cctggccccc gggatgaaga cgctctggcc gggcacgagg acggcggtgc ccaggtcgtt
     2221 cgcgtcggcc aggtcggtga tgcccacctt gtagcgcttc gctatcgagc ccaggctgtc
     2281 gcccttggcc accttgtaga ggatgccgtc catgttcggg atcttgagct ccacgccgac
     2341 gcccaggccc ttcgcgctct tcacgccgtt catcccgacc agggtgtcga cggagaggcc
     2401 gaagcgcttc gctatggcgc cgatcgagtc gccttttttc acgatgtagc tcgagagctc
     2461 cagggtcttg ggcgcgggcg gcggctcgag gccctcgtcc aggctcgcct cggcgagctc
     2521 cggcgaggcg taggcgaaga ggagcgcctc ggcgccttgg tcgtcgtaga ggaaggcccc
     2581 ctcgggccgg gggaaggccg gcggggcgaa gacgaggaag agggcggagg ccagggcgaa
     2641 gaggccgagg cccgagcccg cggcgacgag ccagcgccgt aagcgggcct ccctgccgcg
     2701 gccgcgggag ggctggtcct cccgctccgc cttgggcggc cggctgagga cggaccgcct
     2761 cctcggactc tcctctccgc ggaggacttc gggagcgcgg gacgcgtccg ggcggcggaa
     2821 atccctggag atccggaagg ccttgggaag ccggaaggcc ttgggtaggc gcaggccgag
     2881 gggcgggcga aggccgccgg aggcgcggaa ggccccgccg gccggcttgc cggcctcgcc
     2941 caggaggaag aaatccgcct tgcggacctt ggtcccctcg cgcctggcga agacggaggc
     3001 ctgtccgggg cgcgagagcg acgcctcgcg ggccaggggc ttgaaggggc ggcgcttggc
     3061 gatcttctgt ccggttatga tgctttccat ggctcttacc tggtttatcg acatttgccg
     3121 cgatgggcat tatagttacc gccgattcat gaaacgaagc cgacccaggc cgggagtcct
     3181 catacttctc gccgtcctca gcgccgcgct cctcaaggcc ttcgtcgtcg acctcgccgt
     3241 cgtcgagggc cgctccatgc tccctgccct ggagcagggg gacttggtcc tcgtcctccg
     3301 ctgcgcctac ggcctccgcc tgcccggggc gggcggcgag ggcgcctacc tcctccgctg
     3361 gggacggccc agccccgggg agatcgtcgc ggcggcgagt ccccgggacg gactggccgt
     3421 cgtgaagcgc gtcgccgcgg tggggcccgc ggccctcgtc gtcgaggagg ggcgcctccg
     3481 cggcgcggga ctcgaccttc ccctcgtcgc agcgatggcc gagtccctgg ggcccggcct
     3541 ctacctcggc cgcggctcgg tcttcctcct gggcgacaac gcccccgaat cggtcgattc
     3601 gagggactac ggcgcggttc ccgttgagtc catcgccggg agggtgctat tctggatctg
     3661 atcggcggcg catcgacatg cagaagaacc tgacacgcta ctggctggcc gtcggcatcc
     3721 tcgccgccgc cgccctggtc ctcctgggcc gctacgccgt cctcgccgcg gagaaggggc
     3781 cggccgccgc ccgcgccgcc gcggaggccg agcggggcgc gatcgtcgac cggacgggcc
     3841 gccccctggc catggactcc gccctctaca acatcgccgt ctggcgcccg gagaccgggg
     3901 ccgaggcctt cgcctccgac gccgcccgcc tggcctccat cctcgggatc cccgaggccg
     3961 agatcctcgc caagcgggag tcgggggaga gcgacttctt ctacctgaag aagcgcgtgg
     4021 cgccccaggt cgcccgcgcc gtccaggacg gcaaggcctc cggctccttc gcgggcgtcg
     4081 tcgtggagaa ggtctcgggc aggatctacc ccgagaagcg gctcgcctcc cacctggtcg
     4141 gcttcgtcgg cgacggaaac cgcggcctcg cgggcgtgga gagcaagtac gaggccgagc
     4201 tctcgccgag cccggccccc gcccccgacg cctcctccgg cctcctcaag ggcaacgagg
     4261 tccagctcgc cgtcgactcg gccatccagt tctccctgga ggagatcgcg cggaaggcga
     4321 tggccgacac gggcgcggag tccgtcatcc tcctcgcggg cgacgcgagg acgggcgaga
     4381 tcctggccta cgtcgcgatg cccgacttcg atcccaacga gtactacgcc gcccccgcgg
     4441 aggcctggag cgacctgccc tcggtctacg cctacgagcc cggctcggtc ttcaagatct
     4501 tctccatggc ctccgtgatg gacctggggg gcatcgacgg gtcgaccacc ttcatctgcg
     4561 acggggccta ccggcggaag accccctccg gcgaggagat cgtcatcaag tgcctcggct
     4621 cccacggccc ggtgaacctg gagcgcatcc tcgagctctc ctgcaacgcc ggggcgggct
     4681 acgcctccga caccgtccag tccctcgacc tctacgagcg ccttaccgcc ttcggcttcg
     4741 gggcccgcac ggggatagcc ctcgcggggg agagcccagg cctcctccgc tcgcccgacg
     4801 cctggtccct gcgctccaag ccgaccatcg cgatgggcca ggaggtcctg gtcacggccg
     4861 tgcagatgct ctccgcggcg acggcggtcg cgaacggtgg cgtcctcctc aagcccatag
     4921 ccgtccggaa ggtcgtcgcc ccggacggga gcctggtcta cgagaacgcg ccccaggccg
     4981 tgcgccgcgt cgtgtcggcg gacacggcgg ccgccatcct gggcgcgatg gaggccgcct
     5041 ccggcgaggc cggcacgggc cgccgcgcca aggtcaagga cgtccgcatg gcggtgaaga
     5101 ccggcacggc ccagatggcg gaccgcgcga caaggcgcta ctcggagacc gacttcatcg
     5161 cgagcaccgt cgcgatcttc cccgccgagg cgccgaggat cgtcctctac ctcgcgatcg
     5221 tgaagcccaa gggcgagtcc tactacggcg gccgcatcgc ggcccccgtc gtccgggagg
     5281 ccgcagaggc cgtcctcgcc ctgaccgacc tgccccgcgg ggagagcccg accgtcctcc
     5341 atcccggcgc cgtctccctg ccccgcctgg ccgcgccgac cgtcggcgag acgatgcccg
     5401 acctgcgcgg cacgcccaag cgcctcctcc tccccctcct cgggcgcggg gacctcaagg
     5461 tgaggatcga gggcgagggc tacgtcacgc gccagcgccc cgccccgggc gaggccgtcg
     5521 tccccgggac cgagatcgtc ctggagctcg agtgagcccg gacggggccc cggccttcga
     5581 ccccctcctc ctcgccgcca acctcgaggc cctcgcgggg cgcttccccg ccctggcgcg
     5641 cctcgtcggc gaggccggcg cgccggacct cgagcccttc gccacgcgct cgggcctgcc
     5701 gagcgcccgc ctcgcgggcc ttgccctcca ctcggcctac gaccccgccg ccgaggccgc
     5761 ccgcctggcc gcgaccctcc ccggctcggc cgacaccgtc cttctcctgg gcttcggcct
     5821 gggctacgcc gcggaggcct gcctggcggc gggcggcccc gagcgcgcca tcgtctgcga
     5881 ggcggagccc ggcctccttc gcgccgcgct ggccctgcgc gacctccggc ccctcctcgc
     5941 cgacgagcgc ctcggcttcg tcgtcggggg cgagcccgag ggcctcctcg ccgccctgga
     6001 ggactcgggg gcgaggcgcg cgggcctcct cggcctcaag gcggccgagg ccgcgagccc
     6061 cgactggtac ggccgggcca gggcggccgt ccagcgccac caggccaagg agggcatcaa
     6121 cgagaacacc ttgcggcgct tcggccgcct ctgggtgcgg aacctcgcga agaacctccc
     6181 cgagatcggc cggcttcccg gcgtctcaag gctggagggg cgcttcgcgg gcttccccgc
     6241 cctggtcctg gccgccggcc ccagcctgga cgaggccctg ccgctgatcg gggagctcgc
     6301 cgagcgctgc cttgtcgtct gcgtcgacac cgccctacgg agcctcctcc gggccggcct
     6361 cgagcctgat ttcctcgtcg tcgtcgatcc ccagtactgg aactggcgcc acctggaggg
     6421 cctctcctcg ccgggctcca tcctcgtctc cgagtcggcc gcctggccgg ccgtcttccg
     6481 cttcggcgcc cgggcggcct tcctgggagg cagcctcttc cccctggggc gggccgtcga
     6541 ggcctacgcg agccccaagg gcctcctggg cgccggcggc tcggtcgcga cgagcgcctg
     6601 ggacctggcc cgcctcctgg gcgcctcgcc gatctacatg gccggcctcg acctctcctt
     6661 ccccgggggg gccacccacg cgaaggcgag cctcttcgaa cagcgcgccc tctctggcgg
     6721 gcgccgcctt tctcccgccg acagcgccca ggccgcggcc ctctgctccg ccgacccgag
     6781 cccggcgccc gacaactccg gaggcctcgt gcggacggac cggcgcatgg ccctctacgc
     6841 ctggtggttc gagagccgcc tcgcgcggcc ctcctcgccg cccaccagga gcctctccgc
     6901 ccgcggcctt gccatacccg gcatgcccct cgcctccctg gacgaggtcc tggccctccc
     6961 cccgcgcagg tccgagctgg ccgagaggct cgacgcggcc gcggccctgc gcgaggaggc
     7021 cgaggctccc gcccgcctcc aggaggggcg gctcgctctt ctggccgacc ttgcgcgggt
     7081 ggaggccgtc gcgggcgcgg cggcccaggc ggccgccgcc gcccgcgccg ccctcgcctc
     7141 gggcggcgat ccccgagccg agctggccct cctcgaccgc gccgacgccg aactcctcgg
     7201 caacgcggcg cgggacgtcg cgggcttcct cctccctccc ctgggggagc tcctttcccg
     7261 ccgcgcccgc gacctcgcgg agagcctcgg gcagtccgag gccatctacc gcggggcggc
     7321 agagtcggcc cgctaccacc tccaggttct cgcctaggaa ggggcggcgc atgggcgtcc
     7381 gcctcggtaa aaggccgctt cctagccatg gggcgggcgc ccggtggggc tgccttggcg
     7441 aacgcgcgag cctttctctt ttttttaagg ttttccccga ccctgtcgag aatttgtgta
     7501 gagggaaacc tctaccaacg gtatgcgtac tgacgtccag gcataccgtt tttttatgcc
     7561 ctgacggccg ggacgcggcc gactatctcc aggcccgcct tcggggcgcg gccggagaag
     7621 aggaccttgc ctcccttcga ccattccagc tcgaggacgg cgtcgacgct ctccgctatc
     7681 cggcgcgaga ggaggccgtc caccggcgcc cgcagggagc cgcccctcgc ccgcgcgatc
     7741 cgcgcctcga tcctctcccc gccccgctcg accacgaccc tcgccgaagc ctcgtccgcc
     7801 tggaggaggc cgagcctcgc ccccgtgtag ctcgcctcct ggaggagccg tcccccgagg
     7861 gaggcggcgc agaggaagcc cgtgaaggag ccgccgagcc agggaatcct cgcgacggag
     7921 agcatgaagg agtcgccctc gacggggaag ttgttggact gggtccagag ccagctctcg
     7981 ggcatcgagc ggccccagtc cttctccgcg tagccgcggc cccccgcgaa gtcgatgggg
     8041 cgcccgtcgt ggcggagcga gccggacagg ccgtggtgaa gcgacaccag gccgtgcctg
     8101 cactccatga agggaacgaa ggtgtagggg cccatcacgc cgggggagtg gaggggcgag
     8161 gggaggaggg tcggcgggcc gaatccgagc tccccctcga agcggccttc cggcgaggag
     8221 aggtccaggc gcaggcccga gcgggagaat tcgcagccgc cgaccctcac cgacagctcc
     8281 ctcctggacg cggagaaggc cgagaggggg aactcgaacc accaggagcg ggccgtcctc
     8341 ccctcgatgg cctggacgaa ggaatagccc tcgcccggcc tccgaccccg cgcgacgccg
     8401 ggtatgaagg accaggactc ctcgaggtcc cgggtcacct gcttgaagta tcagccctcg
     8461 aagtagtcgc gccgcctgcg ggctccttgg tatgcctcgg ggcgccaaag cccgaacgcc
     8521 atcggtcctc ctcgcctggc cgcggcctcg cgcggccgcc tgccttgtcg cccggccggg
     8581 cggccgcgcg cctcggtccg ccgccggaat tccccaggcg gcgcggcccg ggcggcgcgg
     8641 cgcggagtcc gccctggcgg tctttttcgc ctcggcatat aataccgccg aattccagtt
     8701 tcccggaggt atcatgcaga gccgcgacga agccatccgc ctctggcggg agcacaacaa
     8761 ggacgagtcc cttttccgcc acgccctctc cgtggaggcg gcgatgcggc acttcgccgc
     8821 caaggcgggg gaggatcccg aatactgggg cctcgtcggc ctcctccacg acatcgacta
     8881 cgaggagcac cccaaggagc acctcaagca cgcgcggccc atcctctccg gagccggcta
     8941 tcccgagccc ttcgtccgcg ccgtggagtg ccacggcttc gggatctgca ccgacatcga
     9001 gcccgtcctc gggatggaga aggtcctctt cgccgtggac gagctcacgg gcttcatcgc
     9061 cgcctgcgcc ttcgtccgcc cctccaagag cgtcctcgac ttggaggtga agtcggtcaa
     9121 gaagaagtgg ggcacggccg ccttcgccgc gggcgtgagc cgcgaggtga tagagaaggg
     9181 cgctgccatg ctcggcctcc ccctcgacga gctcatcgcg gagacgatcc aggccctccg
     9241 gggcgtcgcc gccgaggtcg ggctcaaggg gagcctctag ccgccgccgg cccgtcccgg
     9301 cgctccgcgg tgcgccgggc cccgatctcc gggcacccgg cgtcccgggt cgggccgctc
     9361 gattgacgag gtccggcccc ccgggcgata atcccgcgat ccctggcccc ccgcggggcg
     9421 gcccgggcaa cactctggac agcgaagcga gaaagccatg ctcgagaaga taagccagaa
     9481 attcggcgac gtcgtgcgga cgatcgccgg caagtccgcg atcagcgaga agaacgtcga
     9541 ggaggccgtc gagcagatca agctcgccct cctcg
//
LOCUS       DAOOSJ010000081         9523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4624_length_9523_cov_6.122095, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000081 DAOOSJ010000000
VERSION     DAOOSJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4624_length_9523_cov_6.122095"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLG14_05535"
     CDS             complement(<1..188)
                     /locus_tag="PLG14_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOX48631.1"
                     /translation="MKITVLTLFPEILRGFFESSIMAKAVQKGLLEYELVNIRDYATD
                     KHHKCDDLVFGGGAGMLML"
     gene            complement(185..712)
                     /gene="rimM"
                     /locus_tag="PLG14_05540"
     CDS             complement(185..712)
                     /gene="rimM"
                     /locus_tag="PLG14_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOX48632.1"
                     /translation="MPSDKDPRQQKLAIGHLGAPRGVRGDLRVQSYSGETEHLKRLRY
                     ADLEGGGRSLRLKVLRCEGGPGSLSMAFEGYATPETARALTGMDIFVSREEAAPLREN
                     EWYEVDLVGLALVGSDGKELARVKGVLSGGADPCLEAELPGGKVALVPFRKEFVGEVE
                     VEAGRIVLLAPWILE"
     gene            complement(789..1022)
                     /locus_tag="PLG14_05545"
     CDS             complement(789..1022)
                     /locus_tag="PLG14_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOX48633.1"
                     /translation="MERDLVEYIAKSLVDDPAGVTVNVIEGDKSTILELRVASDDIGK
                     VIGKHGRIAKAMRTILSAASTASEKRVVLEILD"
     gene            complement(1086..1337)
                     /gene="rpsP"
                     /locus_tag="PLG14_05550"
     CDS             complement(1086..1337)
                     /gene="rpsP"
                     /locus_tag="PLG14_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOX48634.1"
                     /translation="MSVKIRLKRFGAKMRPFYRIVIMDSRSPRDSRTIDEIGLYHPIE
                     TEDKQIQIDEAKAKDWLSKGAQPTDTVKSLLNKKGIHVK"
     gene            1503..>2095
                     /locus_tag="PLG14_05555"
     CDS             1503..>2095
                     /locus_tag="PLG14_05555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOX48635.1"
                     /translation="MKPSLPPDALVLAPMVELSHRALRELILGFGGLDLAYTEMASAG
                     AFVADSPYDRWFLDAEPEPARTVLQFYTTGPERLVEALERAADRPVFGADINFGCAAP
                     QIKRAGGGAAWMREPEKAKELVGAARPAWPLSLSAKLRIGPEEDFGALLDFASGLAEA
                     GLDFLTLHPRLENEKFRRKSRWDYVGRLAAALPIPVLG"
     assembly_gap    2096..2195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2255..4165
                     /locus_tag="PLG14_05560"
     CDS             2255..4165
                     /locus_tag="PLG14_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX48636.1"
                     /translation="MAQTIPLMFIEKAKAQPTVAAQFTKDGSGEFAPISYGELLADVE
                     SFAAGLAASGVARGDRVGIISDNRREWLVSDLAILGLGAADVPRGCDATEDEIAYILS
                     FSECALAILENERQLAKLLSRRERLGALKRVLLFDTPSAAAKAEAEKAGFAVSTFAEV
                     DVLGRAEAAKRPGFYLAEAAKGGRDDVATLIYTSGTTGEPKGVMLSHANFLHQTDRIP
                     AIIHLKPGDIFLSVLPIWHSFERIAQYMILASGAGVAYSKPVGSIMLADLQAVRPQWM
                     ASVPRIWESVMEGVYKSIRKQSALKKGLFSFFIGVGETRVFLRDRLLGRVAEFHERSR
                     VLEAAAALIPFLLVSPLAALGNLLVFKKVKDKLGGRFVAGISGGGALPASVDHFFSAL
                     GVLILEGYGLTETAPVLAVRPQDRPVAGTVGPALEGTELKIVDEQGKSLPFGHKGLIM
                     VRGPQVMRGYYKRPDLTERVLSADGWLNTGDLGMLTSRGELKITGRAKDTIVLRGGEN
                     VEPAPIEEKLRESPYVQSAVVLGQDERFLAALVVPKEEAVLAWAEENNVPIVDYESLL
                     SQPEVLELFDGEVSGLVSPHNGFKSFERIFRFVLLPKPFEIGKELSAKQEIKRHAIAE
                     IYSHQIRKLFQG"
     gene            4412..4660
                     /locus_tag="PLG14_05565"
     CDS             4412..4660
                     /locus_tag="PLG14_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOX48637.1"
                     /translation="MNDIKIWICMGSSCYARGNARNAELVQSWLKQKGLDATVEFAGT
                     LCEGRCKEGPILKIGERLFTGVSPASVGEILEHVLGGK"
     gene            4662..>5059
                     /locus_tag="PLG14_05570"
     CDS             4662..>5059
                     /locus_tag="PLG14_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX48638.1"
                     /translation="MDQRQPVYTETAACQDCYKCVRECPVKAIRVSSGHASVVGELCV
                     LCGHCVEVCPVGAKKVRSDVERAKALIELKPRVLLSLAPSFAAEFPGVAPERLVAALR
                     RLGFAAVSETAFGADLVSRATAAELEALRGP"
     assembly_gap    5060..5159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5160..6543
                     /locus_tag="PLG14_05575"
     CDS             <5160..6543
                     /locus_tag="PLG14_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOX48639.1"
                     /translation="AAARRPRVLLSTACPVVVDYVLKYRPALAGLLSASCSPMLAHAR
                     HLKAVSGPGTAVVFAGPCIAKKREADGSMEGPGALGGVEAAVTFRGLRTWLQEEGLDP
                     AALESGPEDRFYPERASKGVLYPVDGGMISSVKRHVRDRDVHFVSYSGLVEIEKALDG
                     LEDYEGEEPIFLELLACEGGCVNGPRCERRSGTVAKRLRVLDYARGAREEAPETGVDL
                     ADARPGRSAVPAESYPEERLREALRLVGKHEAGDELNCSGCGYDSCRAFAAALLSGRA
                     ERTMCVSYMRDLAQKKADALVRAMPSGVVVADAELRVVECNEKFASLLGPDAATAFEA
                     RPGLSGAELERLVPFAELFSRVMSDEGPEYLERDLKIGDRVLHGTVFSIERGAYSGGV
                     FQDVTLPWIQRDRIVAQAKAVIDKNLRTVQRIAYLLGENAADTESTLESIIESFSAAS
                     GKAKDREEAR"
     gene            6540..7736
                     /locus_tag="PLG14_05580"
     CDS             6540..7736
                     /locus_tag="PLG14_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX48640.1"
                     /translation="MKGSFVEIGHYNEAKRGERAEGDVFLSRKSAEEGRLIAVLSDGL
                     GSGVKANVLATLTATMAAGCAASNVPIKRTAGVIMRSLPVCSERRISYATFTLVDVAA
                     SGALSIVEYDNPAYVLVREGSLVEPIKTEIPVKRGRRSGGLGNMAILRWSSFQARPGD
                     RLVVFSDGVTQAGMGSRASPLGWGGPAVEDFVVGAVRAEPGVSARDLAKAVVREALAR
                     DAGAARDDITCGVVYFRDARRLLVLTGPPLYPERDVEMARVFREFQGRRLVAGGTTAS
                     IIARETGAKVKVELGRLDPEIPPAASMEGADLVTEGIVTLGRAAELLSSRERFDALPR
                     NAATRAIDLMLDSDVIEFVVGTKINEAHQDPNMPVELEIRRNVIKRLAAVLGEKFLKD
                     VKIRFI"
     gene            7748..8032
                     /locus_tag="PLG14_05585"
     CDS             7748..8032
                     /locus_tag="PLG14_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOX48641.1"
                     /translation="MSEKVRVVVCTGTACYVMGGAELLDIESRLPAALAGRIELEGSP
                     CLGLCHDRRNGAAPFAEVDGETVAAATIEGLAEAIGRRLAARGGSGREGL"
     gene            8071..>9523
                     /locus_tag="PLG14_05590"
     CDS             8071..>9523
                     /locus_tag="PLG14_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HOX48642.1"
                     /translation="MIRSARLALEGRLAEGADRIPYEMTAEGWETIRCCKHHDRAILR
                     LRTMAVLGADPEGLDDADRPLADCAREALARAAAAPEARKEAVAGRRPLALIGEACNA
                     CVKTRYLVTEACQGCLARPCKMNCPKGALVVEGGRARIDPAKCVNCGLCMKNCPYTAI
                     VKLPVPCEEACPVGAISKGVDGVERIDHDKCVLCGKCVRECPFGAVVERSELVDVALE
                     LKAGKPAVALVAPAVAVQFPGGLGRLKAALRLLGFAAVMEVARGAETTAEREAEELVE
                     RMEKGEGFLATSCCPAWTRTARKHLPALLANVSSTPSPMAFAAELAAREAPGALRVFV
                     GPCAAKRGEAAENPLVDRVLTAEELGALFVAAGVDVGALEPEDATGAAPGARAFAASG
                     GVAAAVAARLSGADAGAASAGAAPRLLVVNGISKQGLKLMETWAAKPPEADLVEVMAC
                     EGGCVGGACVLANPRLAKLELDRMVAAAATSAAA"
BASE COUNT         1349 a         3463 c         3105 g         1406 t
ORIGIN      
        1 agcatgagca tgcccgcccc gccgccgaag accaggtcgt cgcacttgtg gtgcttgtcc
       61 gtcgcgtagt cccggatgtt gacgagctcg tactcgagga ggcccttctg cacggccttg
      121 gccatgatcg agctctcgaa gaagccccgg aggatctccg ggaagagggt gaggacggtg
      181 atcttcattc gaggatccag ggagcgagga gcacgatccg gcccgcctcg acctccacct
      241 cgccgacgaa ttccttgcgg aagggcacca gggccacctt gccgccgggg agctcggcct
      301 ccaggcaggg atcggcgccg ccggagagga cgcccttcac gcgggcgagc tccttcccgt
      361 cggagccgac gagggccagg ccgaccaggt cgacctcgta ccactcgttc tcgcgcaggg
      421 gggccgcctc ctcgcgggag acgaagatgt ccatgcccgt gagcgcccgc gccgtctccg
      481 gcgtggcgta gccctcgaag gccatggaga ggctgccggg gccgccctcg cagcgcagga
      541 ccttgagcct gaggctccgc ccgccgcctt cgaggtcggc gtagcgcagc ctcttgagat
      601 gctcggtctc gccggagtag ctctggacgc ggaggtcgcc ccggacgccg cggggcgccc
      661 cgagatggcc gatcgcgagc ttttgctgcc tgggatcctt gtctgacggc atggtcgcat
      721 ggctccgccg gaagggcgcc ggcccctagc cccagccggg gaagggccgc gccttttcgg
      781 ctccggcctt agtccagtat ctcgaggacg acccgcttct cggaagcggt ggaggccgcg
      841 gagaggatgg tgcgcatcgc cttggcgatg cggccgtgct tcccgatgac cttgccgatg
      901 tcgtcgctcg cgacgcggag ctcgaggatg gtggacttgt ccccttcgat gacgttgacc
      961 gtcacccctg cggggtcgtc caccagggac ttggcgatgt actcgacgag atcgcgttcc
     1021 acggatgtct cctctaggat gcgaagcccg gaagggcggc cttcggaggc cgccgcgacc
     1081 cggccttact ttacgtggat gcccttcttg ttgaggaggc tcttgaccgt gtcggtgggc
     1141 tgggcgccct tggacagcca gtccttggcc ttcgcctcgt cgatctggat ctgcttgtcc
     1201 tcggtctcga tggggtggta gaggccgatc tcgtcgatgg tgcggctgtc gcgcggcgag
     1261 cgggaatcca tgatgacgat gcggtagaag ggacgcatct tggcgccgaa gcgcttgagc
     1321 ctgatcttga cgctcattct ggttgtcctc caaagtggat ttgggcccgc ccttacgact
     1381 tcatgtctcc cggacggggg gcccggagcg ggaaactcgt tcggactgta tacaccgcgg
     1441 aggccccgag cgtcaatcga ggcgctatga acgaccgccc ctcccgttgt atacatggcc
     1501 ccgtgaaacc ctccctccct cccgacgccc tcgtcctggc ccccatggtg gagctctccc
     1561 atcgggcgct ccgggagctc atcctcggct tcggcggcct ggacctggcc tacaccgaga
     1621 tggccagcgc cggcgccttc gtcgccgact cgccctacga ccgctggttc ctggacgccg
     1681 agcccgagcc cgcaaggacg gtgctccagt tctatacgac cggccccgag cgcctcgtcg
     1741 aggcccttga gcgggcggcc gaccgtcccg tcttcggcgc ggacatcaac ttcggctgcg
     1801 ccgcgcccca gatcaagcgg gcgggcggcg gggcggcctg gatgcgcgag cctgagaagg
     1861 cgaaggagct cgtcggcgcc gcccgcccgg cctggcccct ctccctctcc gccaagctca
     1921 ggatcggccc ggaggaggac ttcggggccc tcctcgactt cgcctcgggc ctggccgagg
     1981 ccggcctcga cttcctcacc ctccacccga ggctggagaa cgagaaattc aggcggaaga
     2041 gccgctggga ctacgtcggc cgcctggccg cggccctccc catccccgtc ctgggnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttgt gtcttgcaca gtattacata
     2221 tttcgttatt atgtaattct caggaggata cgacatggcc cagaccattc ccctcatgtt
     2281 catcgagaag gcgaaggccc agccgaccgt cgccgcccag ttcacgaagg acggctccgg
     2341 cgaattcgcc cccatctcct acggggagct cctggccgac gtcgagtcct tcgccgccgg
     2401 gctcgccgcc tcaggcgtgg ccaggggcga ccgcgtcggc atcatctccg acaacaggcg
     2461 cgagtggctc gtctctgacc tcgccatcct gggcctgggc gccgccgacg tcccgcgagg
     2521 ctgcgacgcg acggaggacg agatagccta catcctctcc ttcagcgagt gcgccttggc
     2581 catcctggag aacgagcgcc agctcgccaa gctcctctcg aggcgcgagc gcctgggcgc
     2641 cctgaagcgc gtcctcctct tcgacacgcc ttcggccgcg gccaaggccg aggcggagaa
     2701 ggcgggcttc gccgtctcca ccttcgccga ggtcgatgtc ctgggccgag ccgaggccgc
     2761 gaagcgaccc ggcttctacc tcgcggaggc ggcgaagggc ggacgcgacg atgtcgcgac
     2821 cctcatctac acctcgggga cgacgggcga gcccaagggc gtcatgctct cgcacgccaa
     2881 cttcctccac cagaccgacc gcatccccgc catcatccac ctgaagccgg gcgacatctt
     2941 cctctccgtc ctgccgatct ggcactcctt cgagcgcatc gcccagtaca tgatcctcgc
     3001 ctccggcgcc ggcgtcgcct actccaagcc cgtcggctcg atcatgctcg ccgacctcca
     3061 ggccgtgcgc ccccagtgga tggcctccgt gccccgcatc tgggaatcgg tgatggaggg
     3121 cgtctacaag agcatccgca agcagagcgc cctcaagaag ggcctcttct ccttcttcat
     3181 cggcgtgggc gagaccaggg tcttcctccg cgaccgcctc ctgggccgcg tcgcggaatt
     3241 ccacgagcgc tcccgcgtcc tcgaggccgc cgccgccctc atacccttcc tcctcgtaag
     3301 ccccctcgcc gccctgggga acctcctcgt cttcaagaag gtcaaggaca agctcggcgg
     3361 ccgcttcgtc gccggcatct cgggcggcgg cgccctcccc gcctccgtcg accacttctt
     3421 ctcggccctg ggcgtcctca tcctggaggg ctacggcctc acggagacgg cgcccgtcct
     3481 cgcggtcagg ccccaggaca ggccggtggc cggcaccgtc ggcccggccc tggagggcac
     3541 ggagctcaag atcgtcgacg agcagggcaa gagcctcccc ttcgggcaca agggcctcat
     3601 catggtccgc gggccccagg tgatgcgcgg ctactacaag cggcccgacc tcacggagcg
     3661 ggtcctctcc gccgacggct ggctcaacac gggagacctc ggcatgctca cgagccgcgg
     3721 cgagctcaag atcacgggcc gcgccaagga cacgatcgtc ctgcgcggcg gcgagaacgt
     3781 ggagcccgcc cccatcgagg agaagctccg cgagagcccc tacgtccaga gcgccgtggt
     3841 cctgggccag gacgagcgct tcctggccgc cctcgtcgtc cccaaggagg aggccgtcct
     3901 ggcctgggcc gaggagaaca acgtgcccat cgtcgactac gagagcctgc tttcccagcc
     3961 ggaggtcctc gagctcttcg acggcgaggt gtccggactc gtctcccccc acaacggctt
     4021 caagagcttc gagcggatct tccgcttcgt ccttctcccc aagcccttcg agatcggcaa
     4081 ggagctctcc gcgaagcagg agatcaagcg ccacgccatc gcggaaatct actcccacca
     4141 gatacgcaag ctcttccagg gctgatcgag cgcagggccg ccccgccggg gcggccctgc
     4201 cttttccagc ggccaggccg cccctttgct cggctccggg cgcccggagc ccgcgcgtta
     4261 cttcgccgtg cccctcgccc tgccgatgcc gcctttcgcc ccgccggcca cgaccttccc
     4321 tatccatccc gccattttcc agatattctt tgtctttctc tgccatccat tgacataatg
     4381 acttctttat tatatataat tccttaacga tatgaacgac ataaagatct ggatatgcat
     4441 gggaagctcc tgctacgccc gcggcaacgc gaggaacgcc gaactggtgc agtcctggct
     4501 caaacagaaa gggctcgacg cgacggtcga gttcgccggc accctctgcg agggccgctg
     4561 caaggaaggc cccatcctca agatcggcga acgcctcttc acgggcgtga gcccggcctc
     4621 ggtcggggag atcctcgagc acgtcctcgg agggaagtga catggaccag cgccagcccg
     4681 tctacacgga aacggcagcc tgccaggact gctacaagtg cgtccgcgaa tgcccggtga
     4741 aggccatcag ggtgagctcg ggccacgcga gcgtcgtggg cgagctctgc gtcctctgcg
     4801 ggcactgcgt cgaggtctgc cccgtcgggg ccaagaaagt gcgctccgac gtggagcggg
     4861 ccaaggccct catcgagctc aagccccgcg tcctcctctc cctggccccc tccttcgccg
     4921 cggagttccc cggcgtggcg cccgaacgcc tcgtcgccgc cctgcgccgc ctcggcttcg
     4981 ccgccgtctc ggagacggcc ttcggggccg acctggtctc gcgggcgacg gccgccgagc
     5041 tcgaggccct gcgcgggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5161 gccgccgcgc ggcgcccccg ggtcctcctc tctaccgcct gccccgtcgt cgtcgactac
     5221 gtcctcaaat accggcccgc cctggccggc ctcctctcgg cctcctgctc gccgatgctc
     5281 gcccacgccc gccacctcaa ggccgtctcc ggtcccggca cggccgtcgt cttcgccggc
     5341 ccctgcatcg cgaagaagcg cgaggccgac ggctcgatgg aaggcccggg cgccttaggc
     5401 ggcgtggagg cggccgtcac cttccgcggc ctcaggacct ggctacagga ggagggcctc
     5461 gaccccgccg ccctcgagtc ggggccggag gaccgcttct accccgagcg ggcctccaag
     5521 ggagtcctct accccgtgga cggcggcatg atctcttccg tgaagcgcca cgtccgcgac
     5581 cgcgacgtcc acttcgtgtc ctactccggc ctcgtggaga tcgagaaggc cctggacggc
     5641 ctggaagact acgagggcga ggagccgatc ttcctcgagc tccttgcctg cgagggcggc
     5701 tgcgtcaacg gcccccgctg cgaacgccgc tcgggcaccg tcgcgaagcg cctccgcgtc
     5761 ctggactacg cccgcggggc gagggaggag gcgcccgaga ccggcgtcga cctcgcggac
     5821 gcgcggccgg ggcggagcgc cgtgcccgcg gagagctacc ccgaggagag gctccgcgag
     5881 gcgctcaggc tcgtgggcaa gcacgaggcg ggggacgagc tcaactgctc gggctgcggc
     5941 tacgacagct gccgcgcctt cgccgcggcc ctcctctccg gccgcgccga gcgcacgatg
     6001 tgcgtctcct acatgcgcga cctcgcgcag aagaaggcgg acgccctggt gcgggcgatg
     6061 ccctccggcg tcgtcgtcgc ggacgcggag ctccgcgtcg tcgagtgcaa cgagaagttc
     6121 gcgagcctcc tcggccccga cgcggcgacg gccttcgagg ccaggcccgg cctctcggga
     6181 gccgagctcg agcgcctcgt ccccttcgcc gagctcttct cccgcgtgat gtcggacgag
     6241 gggcccgagt acctggagcg ggacctcaag atcggcgacc gggtcctcca tggcaccgtc
     6301 ttctccatcg agcggggagc ctattcgggc ggcgtcttcc aggacgtcac cctgccctgg
     6361 atccagcgcg accggatcgt ggcccaggcc aaggcggtca tcgacaagaa cctccgcacc
     6421 gtccagagga tcgcctacct cctgggcgag aacgccgcgg acaccgagtc caccctcgag
     6481 tccatcatcg agtccttctc cgccgcctcc ggaaaggcga aggacaggga ggaggccagg
     6541 tgaagggctc cttcgtcgag atcgggcact acaacgaggc gaagcggggc gaacgggcgg
     6601 agggcgacgt cttcctctcc cgcaagtccg cggaggaagg caggctcatc gccgtcctct
     6661 ccgacggcct cggctccggc gtcaaggcca acgtcctcgc gaccctcacc gcgacgatgg
     6721 ccgcgggctg cgccgcctcc aacgtgccca tcaagcgcac ggcgggcgtc atcatgcgga
     6781 gcctccccgt ctgctccgag cggcggatca gctacgccac cttcaccctc gtcgacgtgg
     6841 cggcctcggg agccctgagc atcgtcgagt acgacaaccc cgcctacgtc ctggtccgcg
     6901 aggggagcct cgtcgagccc atcaagacgg agatccccgt gaagcggggc cgccgctcgg
     6961 ggggcctcgg gaacatggcc atcctccgct ggtcctcctt ccaggccagg cccggcgacc
     7021 gcctcgtcgt cttctccgac ggcgtcaccc aggcgggcat gggctccagg gcgagccccc
     7081 tgggctgggg cgggcccgcc gtcgaggact tcgtcgtcgg cgccgtccgg gccgagcccg
     7141 gcgtctccgc ccgcgacctc gcgaaggccg tcgtccggga ggccctggcc cgcgacgcgg
     7201 gggccgcccg cgacgacatc acctgcggcg tcgtctactt ccgcgacgcg aggcgcctcc
     7261 tcgtcctcac gggcccgccc ctctaccccg agcgcgacgt cgagatggcg cgggtcttcc
     7321 gcgagttcca ggggaggcgc ctcgtcgcgg gcggcacgac ggcctccatc atcgcccgcg
     7381 agacgggcgc caaggtgaag gtcgagctcg gccgcctcga tcccgagatc ccgcccgccg
     7441 cctccatgga gggcgcggac ctcgtcacgg agggcatcgt caccctgggc agggccgccg
     7501 agctcctctc gagccgcgag cgcttcgacg ccctgccgcg caacgcggcg accagggcca
     7561 tcgacctcat gctggactcc gacgtcatcg agttcgtcgt cggcacgaag atcaacgagg
     7621 cccaccagga cccgaacatg cccgtggagc tcgagatccg ccgcaacgtg ataaagcgcc
     7681 tggccgcggt cctgggcgag aaattcctca aggacgtgaa aatccgcttc atctgaagga
     7741 gagagccgtg agcgaaaagg tacgcgtcgt cgtctgcacc ggcaccgcct gctacgtgat
     7801 gggcggggcc gagctcctcg acatcgagtc gaggcttccc gcggccctcg cggggaggat
     7861 cgagctcgag ggctcgccct gcctggggct ctgccacgac cggaggaacg gcgccgcgcc
     7921 cttcgccgag gtcgacgggg agacggtcgc ggccgcgacc atcgagggcc tggccgaggc
     7981 cataggacgc cggctggccg cgcgcggcgg gtccgggagg gaggggctgt gaactacaac
     8041 aacaacgcgt cgcgactcaa gcgcgaggtc atgatccgga gcgcccgcct cgccttggag
     8101 ggccgcctgg ccgagggagc cgaccggatc ccctacgaga tgacggccga gggctgggag
     8161 acgatccgct gctgcaagca ccacgaccgg gccatcctgc gcctcaggac catggccgtc
     8221 ctcggggccg accccgaggg cctggacgac gccgaccgcc ccctcgccga ctgcgcccgc
     8281 gaggcccttg cccgcgccgc cgccgcgccc gaggcgcgga aggaggccgt cgcgggccgg
     8341 aggcccctgg ccctgatcgg cgaggcctgc aacgcctgcg tgaagacccg ctacctcgtc
     8401 accgaggcct gccagggctg cctggcccgg ccctgcaaga tgaactgccc caagggcgcc
     8461 ctcgtcgtgg agggcggccg cgcgcgcatc gaccccgcga agtgcgtcaa ctgcgggctc
     8521 tgcatgaaga actgccccta caccgcgatc gtgaagctcc ccgttccctg cgaggaggcc
     8581 tgccccgtcg gcgccatctc caagggcgtg gacggcgtgg agcggatcga ccacgacaag
     8641 tgcgtgctct gcggcaagtg cgtccgcgag tgccccttcg gcgccgtcgt cgagcgctcc
     8701 gagctcgtcg acgtggccct cgagctcaag gcggggaagc ccgccgtcgc cctcgtcgcc
     8761 cccgccgtcg ccgtccagtt cccgggcggc ctcgggcggc tcaaggccgc cttgcggctc
     8821 ctcggcttcg cggcggtgat ggaggtggcc aggggcgcgg agacgacggc ggagcgcgag
     8881 gccgaagagc tcgtggagag gatggagaag ggcgagggct tcctcgcgac ctcctgctgc
     8941 cccgcctgga ccaggacggc gaggaagcac ctgcccgccc tcctcgcgaa cgtgtcgtcg
     9001 acgccgagcc ccatggcctt cgcggccgag ctcgcggcgc gcgaagcccc cggcgccctg
     9061 cgcgtcttcg tcgggccctg cgccgccaag cgcggggagg ccgcggagaa ccccctggtg
     9121 gaccgggtcc tcaccgccga ggagctcggc gccctcttcg tcgcggccgg cgtcgatgtc
     9181 ggcgccctgg agcccgagga cgctaccgga gccgccccgg gggcgcgggc cttcgccgcc
     9241 tcgggcggcg tcgccgcggc cgtggccgcc cgcctatccg gcgcggacgc cggcgccgcc
     9301 tcggccggag cggcccccag gctcctcgtg gtcaacggca tctcgaagca gggcctcaag
     9361 ctcatggaga cctgggccgc gaagccgccc gaggccgatc tcgtggaggt gatggcctgc
     9421 gagggcggct gcgtcggggg cgcctgcgtc ctcgcgaatc cccgcctggc caagctcgag
     9481 ctcgaccgga tggtggccgc cgccgcgact tcggccgccg cta
//
LOCUS       DAOOSJ010000082         9486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4650_length_9486_cov_6.958647, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000082 DAOOSJ010000000
VERSION     DAOOSJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4650_length_9486_cov_6.958647"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLG14_05595"
     CDS             complement(<1..1662)
                     /locus_tag="PLG14_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX48643.1"
                     /translation="MLIDLPDEAEARSLAAAFSSAGVPWASAAALEAGEGGGAAAPGG
                     TGSARPEASEALRASGAQFLLADARDWPRLTATLPKPPKSYLILLLCDASEAAEAHGL
                     LASGAFDCLLRDGPGWEGRVAAYAKALDSLRRRFSGAFGALERRYEDLVHALPDVVYE
                     LDYDGRITFVNNSVRLLGYEPSELAGRHFSVLLSEEDARAVDRDSVLELFRGERTGPA
                     LSPKLFNERRGLDRRTEGLEVRLRRNPAAGPGGDILASVTAYGEVTAAGEYGMREGRE
                     SFLGSVGVIRDITLRRKSEEMLRKLYQAVDQLSAGVLVSDRRFVVEYANPAFLRITGR
                     SPEEVIGAELFSFFRIAPSDADAMRGLVLDGFDAGGELEIGKAGGGTAWAACRASPVR
                     SPAGDVTHAVAICEDVSQRRAMEELLRVAKEAAEKADRAKSDFLASMSHELKSPVASI
                     LAAARLVEMGSPDPAARARSIIASAEGLLGVLGDILDFVRFETGQASLRKFSFPLPGF
                     VARTCEPFRAAAEAKGLAFEAGKIPADLVESDPDRLGRAFAAILDN"
     gene            complement(1783..2700)
                     /locus_tag="PLG14_05600"
     CDS             complement(1783..2700)
                     /locus_tag="PLG14_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX48644.1"
                     /translation="MLSLTIWGDRGSMPTPGPDTVVFGGNTSCLEIRAGSRLVIVDAG
                     SGIRQLGDKLMREDLKKGPIDTDIFITHTHWDHIMGFPMFTPIYVPGTRLRIHGPITY
                     EDDTLEQVLGSQLSYRYWPVRQEELAAKIEYEQIKETTIDLGEGLTVRTKYLNHPILC
                     LGYRFEYEGKSIVTAYDNEPFRNVFPTDPADPRYDEEAAREGDAAAAEENEKLLRFYR
                     GADILVHDTQYTAKEYVAGKIGWGHSSYEFAVNAAHKTGVKKLLLFHHDPNRSDAELQ
                     ELELFYQERLRGRTGLQAEMAREGKTYQA"
     gene            complement(2711..3580)
                     /locus_tag="PLG14_05605"
     CDS             complement(2711..3580)
                     /locus_tag="PLG14_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOX48645.1"
                     /translation="MQGSDSLRRVKEYVGGMPSLPTTVAKVLEVCNNPKTSPIDLDNV
                     IRLDPVLMGRVLKLINSAYYGLGTQVTSLARAIIMLGINTVKNLALSTAVIDRLSDKG
                     SFRAINMDGFWRHSLGVGVTAKLIAKRRGVDPKSVEEYFAAGLLHDVGKIPLNNALAE
                     DYIRTMAVADRERIPLFLAEKRALDLDHCEAGRVVSESWRLGGAIGDAITWHHTHESY
                     RGDFGDVVRTVAVANYFANREGIGFSGNLYPDKPGAEVYGLLDMEKSWLEELEAPVAA
                     EIEKAKVFLKLAQ"
     gene            complement(3564..6809)
                     /locus_tag="PLG14_05610"
     CDS             complement(3564..6809)
                     /locus_tag="PLG14_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48646.1"
                     /translation="MSSSATKNPAQLLERARGALRLRDFESAERLARQFLLERPESDE
                     ARLLLGGVYQRTGRAQDALSAFASVIARDAGNIEALIGIGEAYLKLGKPKDAIGALER
                     ALGAAPENAEILFSLGNAHRQTGNLKAAEMSYAKAIEIDPSHVLAYNNLGGVYRAMGD
                     PEKAVRVFWRGLQVDPNYPSFHYNLGYAYDALGKHEEALKEYEQAVKARPGWVEAMNR
                     YGLALLHTGKHRRSMDVFTQVLAVDPLNAEARNDIGLVHAEEGRVDDALRWFREAIEA
                     DPSFIRAALNIEQLLEKAGRWDAALQEIVRLVEIMPENDELRVHLASLYENLGRGREA
                     REALEEVLRRRPDEPKALKPYAALLEKAGESDKATAIYEKLLRLDPGEVGFRVELARV
                     AAARGDLARAEKELLSYLRARPEDRAARLELARARAKRGDHEGAKATLLDLSREDPDD
                     PEVQLELSAAYRALGEGSKAVEAAERLVTLRGARGGETVDDLLSGIGVYENAVRNYGA
                     GMTGAWERNIAVLRKARGAERDAAAAEEAAEAGAAPEGDASLQPFLSMTAAEPLAVPE
                     EEELLFLDEREEPLDEDILEEGEEPVFEEEDEEPRPRGLDALVSPQDRRDAEPPFRPA
                     PQAEPPLTPRAEGQAEPWREPARPEPAPRYAPPPEAPQAQPSYAPPPQMQMPPMQMPQ
                     MQMPPMQMPPIQVMMPPMPAAPAYRPEPPRRRPSGPIELSQPEPYEEEDLGFDDLPLP
                     EGGEEEIEDLEEYIEEPYLEGEEPFDDDEFDLGTDLEPEPEPEPESEPEPEPEPEAEA
                     ASEAAGEDGGGLLMEEEAEDIWNGEEPGGEELGEEEILPAAEEAPPPESEPAPESDSQ
                     PTATPEAAAEAGSPAGPPSGAADEGGQPAASEESLLGERAAKLFEYLKGLSEELPEEK
                     RNEFEESGLREKLDGLIGKLAPEAEEEGGAEAGPETAGQGAAGPVSEGGPSGLLATAA
                     AARPHDPRRAPEGRRSGRERRAPQSNRRSGEPDRRRLGDRRQADDRRAELDRRSEPPK
                     LELPESIPPEAAPVRTSPEGRPLEIAGLPVSPRIARLIEIMRREKENAGKR"
     gene            complement(6806..8494)
                     /locus_tag="PLG14_05615"
     CDS             complement(6806..8494)
                     /locus_tag="PLG14_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48647.1"
                     /translation="MSNKGIAFLAILLAAVSAATVPAGANPGEAFLERIALTPGGGAL
                     RLGPVQPARAGAPGAAGEASGAASRVPLVGSWTADGAVALPGRGGGLDYVLSHAAYRA
                     DEASDLLLHFDGGAPRDEAGNYEAEAGPGFSVEGPKSLLGGGSASFRGKGSAVSLRPR
                     SGALFGKDSRFRDFSVEFWLYPVNAENGEVILLWQSLRKLPGGVLPQRLSCVVAAGRV
                     SWGFENFFERPVAGKAGPAEAATRLDLRARSPLVPRAWSHHLLRFDGDTGLLEYLVDG
                     RPEATAYATPSGREAGPGGAVFAPAAGAASPLVVGEGYAGLMDELRISRRFVEKPSLS
                     PYGRDPGLVLSPVVDLGFGHSRLASIEAVARTPGNSSVELSYRLAEDWAGWRLDYPAW
                     TPFRPGEALPAGARGRYLQVRADLYADGTGRLGPALSSIAVAYEPDPPPPPPARLAGL
                     PKDGAAELRWTRVPESDVAGYLVYYGDAPGEYFGVGAAEGPSPVDAGLSTTLTLTGLA
                     NGRLVYVAVAAYDSAARAGAGPGTAGAAASASAAASRAGEFSTEVSVRPSRTAR"
     gene            8522..>9486
                     /gene="uvrC"
                     /locus_tag="PLG14_05620"
     CDS             8522..>9486
                     /gene="uvrC"
                     /locus_tag="PLG14_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOX48648.1"
                     /translation="MADNKAGRKPHREGEAADSPSKDRKAGLKALVRQSPLEPGVYIM
                     RDASGSIIYVGKAKVLRNRLSSYFTGRKDVKTRHLVSRIESIEWIVSPSEYEALLLEN
                     NLIKENSPKYNINLKDGKTYPSVRVTNEPFPRVFRTRRIVNDGSSYFGPFPSAETIDT
                     YLDLIRRLFPLRRCSAMKKREAPCMYYHIGRCSAPCAGKIGEAEYALHVEEVKKLLSG
                     ETEALLSELRERMAEAAAALRFEEAGRLRDAVQAIELFAGRSGAVDFDPGARDYVAQA
                     ADGDLVVFAAFQMRGGKLTGRDVYRSRLYGGEEEALEAFLMAYYGG"
BASE COUNT         1218 a         3255 c         3605 g         1408 t
ORIGIN      
        1 gttgtccagg atggccgcga aggcccgtcc caggcggtcg gggtcgctct cgaccaggtc
       61 ggccgggatc ttccccgcct cgaaggccag gcccttggcc tcggcggccg cgcggaaggg
      121 ctcgcaggtc ctcgcgacga agccgggcag ggggaaggag aacttccgca gggaggcctg
      181 gccggtctcg aagcgcacga agtccaggat gtcgccgagg actcccagga ggccctcggc
      241 gctcgcgatg atggagcggg cccgcgcggc ggggtcgggg gagcccatct ccacgaggcg
      301 ggcagcggcc aggatgctcg ccacggggct cttgagctcg tggctcatgg aggcgaggaa
      361 gtcgctcttg gcgcggtcgg ccttctcggc ggcctccttc gcgacgcgga ggagctcctc
      421 catcgcccgg cgctggctca cgtcctcgca gatcgccacc gcgtgggtca cgtcgcccgc
      481 gggcgagcgc acgggcgagg cccggcaggc ggcccaggcc gtgccgcccc cggccttccc
      541 gatctccagc tccccgcccg cgtcgaagcc gtccaggacg aggccgcgca tcgcgtccgc
      601 gtccgagggc gcgatccgga agaaggagaa gagctccgcg ccgatgacct cctcgggcga
      661 gcggcccgtg atccggagga aggccgggtt ggcgtactcc acgacgaagc gccggtcgga
      721 gactaggacg ccggcggaaa gctggtccac ggcctggtag agcttccgga gcatctcctc
      781 ggacttgcgc cgcaaggtga tgtcgcgtat gacgcccacg gagccgagga aggactcgcg
      841 gccctcccgc atgccgtact cgccggccgc ggtgacctcg ccgtaggcgg tgacggaggc
      901 gaggatgtcc ccgccggggc cggccgccgg attgcggcgc agccgcacct ccaggccctc
      961 ggtgcgccgg tccagccccc gccgctcgtt gaagagcttg ggcgagaggg cggggcccgt
     1021 cctctcgccg cggaagagct ccaggaccga gtcccggtcc acggcgcggg cgtcctcctc
     1081 ggagaggagg acggagaagt gcctgcccgc cagctccgag ggctcgtagc cgaggaggcg
     1141 gaccgagttg ttcacgaagg ttatgcggcc atcgtagtcg agctcgtaga ccacgtcggg
     1201 cagggcgtgg accaggtcct cgtagcgccg ctccagggcg ccgaaggccc cggagaagcg
     1261 gcggcgcagg gaatcgaggg ccttggcgta ggccgcgacc ctcccctccc agccgggccc
     1321 gtcgcggagg aggcagtcaa aggcccccga ggcgaggagg ccgtgcgcct cggcggcctc
     1381 ggaagcgtca cagaggagga gtatgaggta ggacttgggc ggcttcggga gggtggccgt
     1441 caggcggggc cagtccctcg cgtcggcgag gaggaactgg gcgcccgagg cgcggagggc
     1501 ctctgaggcc tcgggcctgg ccgatccggt cccgcccggg gcggccgcgc cgccgccctc
     1561 gccggcctcc agggccgcgg cggaggccca gggcacgccg gcggaggaga aggccgccgc
     1621 gaggctgcgg gcctcggcct cgtccggcag atcgatgagc accgctggcc tccgagcttc
     1681 gtccatggct tccacgttcg tccttcgcgg ccgcgaaatc aagggccgcc ttgagggcgc
     1741 ggtccgcgcc tccgcgccgc gcgccgcccc gagcccaggc ccctaggcct ggtaggtctt
     1801 gccctcgcgg gccatctccg cctgcaggcc cgtcctgccg cggagccgct cctggtagaa
     1861 gagctcgagc tcctggagct cggcgtcgct ccggttcggg tcgtggtgga agaggaggag
     1921 cttcttcacc cccgtcttgt gcgccgcgtt cacggcgaac tcgtaggagg agtggcccca
     1981 gccgatcttg cccgcgacgt actccttggc cgtgtactgc gtgtcgtgga cgaggatgtc
     2041 ggccccgcgg tagaagcgga ggagcttctc gttctcctcc gccgcggcgg cgtcgccctc
     2101 gcgggcggcc tcctcgtcgt agcggggatc ggcggggtcc gtcgggaaga cgttgcggaa
     2161 gggctcgttg tcgtaggcgg tgacgatgga cttcccctcg tactcgaagc ggtagccgag
     2221 gcagaggatg gggtggttca ggtacttcgt ccgcaccgtg aggccctccc ccaggtcgat
     2281 cgtcgtctcc ttgatctgct cgtactcgat cttggccgcg agctcctcct gcctgaccgg
     2341 ccagtagcgg taggagagct gcgagccgag gacctgctcc agggtgtcgt cctcgtaggt
     2401 gatggggccg tggatgcgga gcctcgtgcc gggcacgtag atgggcgtga acatgggaaa
     2461 gcccatgatg tggtcccagt gcgtgtgcgt gatgaagatg tccgtgtcga tcgggccctt
     2521 cttgaggtcc tcgcgcatga gcttgtcgcc cagctgccgg atccccgagc ccgcgtcgac
     2581 gatgacgagg cgcgagccgg cgcggatctc caggcaggag gtgttgcccc cgaagaccac
     2641 cgtgtcgggg ccgggcgtcg gcatggagcc gcggtctccc cagattgtca ggctcagcat
     2701 tcggctctcc tcactgtgcg agcttcagga agaccttggc cttctcgatc tccgccgcga
     2761 ccggcgcctc gagctcctcc agccaggact tctccatgtc gaggaggccg tagacctcgg
     2821 cgccgggctt gtcgggatag aggttgcccg agaagccgat gccctcgcgg ttcgcgaaat
     2881 agttggccac ggcgacggtg cggaccacgt ccccgaagtc gccgcggtag ctctcgtgcg
     2941 tgtggtgcca ggtgatggcg tcgcctatcg cgccgcccag gcgccagctc tcggagacga
     3001 cgcggccggc ctcgcagtgg tccaggtcca gggcgcgctt ctccgccagg aagaggggga
     3061 tgcgctcgcg gtcggccacg gccatggtgc ggatgtagtc ctcggccagg gcgttgttca
     3121 gcggtatctt cccgacgtcg tggaggaggc cggcggcgaa gtactcctcc accgacttgg
     3181 ggtcgacgcc ccggcgcttc gcgatgagct tggcggtcac gccgacgccc agggagtgcc
     3241 tccagaagcc gtccatgttg atggcgcgga aggagccctt gtcggagagg cggtcgatga
     3301 cggccgtgga cagggcgagg ttcttcaccg tgttgatgcc gagcatgatg atggcccgcg
     3361 cgaggctcgt gacctgggtg ccgaggccgt agtaggcgga gttgatcagc ttgaggaccc
     3421 tgcccatgag gacggggtcg agcctgatca cgttgtcgag gtcgatgggg ctggtcttcg
     3481 gattgttgca gacctcgagg accttggcga cggtcgtggg aaggctcggc atgcccccga
     3541 cgtattcctt gaccctgcgg aggctatcgc ttccctgcat tctctttctc ccggcgcatg
     3601 atctcgatga gccgcgctat gcgcggggaa acgggaaggc cggcgatctc gagcggcctg
     3661 ccctcgggcg acgtgcggac cggcgccgcc tcgggcggga tgctctccgg cagctcgagc
     3721 ttgggcggct cggagcggcg atccagctcg gcccgccggt cgtcggcctg gcgcctgtcg
     3781 cccagcctcc tgcggtcggg ctcgccggag cggcggttgg actggggagc cctgcgctcg
     3841 cggccggagc gccggccctc gggcgcgcgg cgcgggtcgt ggggcctcgc cgcggcggcg
     3901 gtcgccagga ggccgctcgg tccgccctcg gacaccggcc cggccgctcc ctgaccggcc
     3961 gtttccggcc cggcctccgc cccgccctct tcctccgcct cgggcgcgag cttccctatg
     4021 aggccgtcca gcttctcgcg gaggccgctc tcctcgaatt cgttgcgctt ctcctcgggg
     4081 agctcctcgg agagcccctt caggtactcg aagagcttgg ccgcccgttc gccgaggagg
     4141 gactcctcgg atgcggcggg ctggccgcct tcgtccgcgg cgcctgaggg ggggccagcc
     4201 ggggatccgg cttcggcggc cgcctcgggc gtcgccgtcg gctgggaatc ggactcgggc
     4261 gccggctcgg actcgggagg cggcgcctcc tcggccgcgg ggaggatctc ctcctcgccc
     4321 agctcttctc ctcccggctc ctccccgttc cagatgtcct cggcttcctc ctccatgagg
     4381 aggccgcctc cgtcttcgcc cgccgcctcc gaagcggctt cggcctcggg ttcgggttcg
     4441 ggttcgggtt cggattcggg ctcgggttcg ggctcgggct ccagatcggt accgagatcg
     4501 aactcgtcgt cgtcgaaggg ctcctcccct tccaggtagg gctcctcgat gtactcctcg
     4561 aggtcctcga tctcctcctc gccgccctcg gggaggggga gatcgtcgaa gccgaggtcc
     4621 tcttcctcgt agggctcggg ctgggagagc tcgatggggc cggaggggcg acgacgcggc
     4681 ggctcgggcc tgtaggccgg ggccgccggc atgggaggca tcatcacctg gatgggcggc
     4741 atctgcatcg gaggcatctg catctggggc atctgcatcg gaggcatctg catctggggc
     4801 ggaggcgcgt aggagggctg cgcctgcggc gcctcgggcg gcggcgcgta gcgcggggcg
     4861 ggctcgggac gggcgggctc ccgccagggc tcggcctggc cttcggcgcg gggagtcagg
     4921 gggggctccg cctggggggc gggccggaag ggcggctcgg cgtcgcgccg gtcctggggg
     4981 ctcaccaggg catcgaggcc gcggggcctc ggctcctcgt cctcctcctc gaagacgggc
     5041 tcctcgccct cctccaggat gtcctcgtcc aggggctcct cgcgctcgtc gaggaagagg
     5101 agctcctcct cctcgggcac ggcgaggggc tcggcggccg tcatcgagag gaaaggctgg
     5161 agggaggcgt cgccctcggg ggccgcgccg gcctccgcgg cctcctcggc cgcggccgcg
     5221 tcgcgctcgg cgccgcgggc cttcctgagg acggctatgt tcctctccca ggcgccggtc
     5281 atgccggcgc cgtagttccg cacggcgttc tcgtagacgc cgatgccgga gaggaggtcg
     5341 tcgacggtct cgccgcccct ggcgccccgg agggtcacga ggcgctccgc ggcctccacg
     5401 gccttcgagc cctcgccgag ggcgcgatag gcggcggaga gctcgagctg gacctcgggg
     5461 tcgtcgggat cctcgcggga gaggtcgagg agggtcgcct tggccccctc gtggtcgccg
     5521 cgcttggcgc gcgcgcgggc cagctcgagc cgcgcggccc tgtcctcggg gcgggcgcgg
     5581 aggtaggaga ggagctcctt ctccgcccgc gccaggtccc cccgcgcggc cgcgacgcgg
     5641 gcgagctcca cgcggaagcc cacctcgccc gggtcgagcc tgaggagctt ctcgtagatg
     5701 gcggtcgcct tgtcggactc gccggccttc tccaggaggg cggcgtaggg cttcagggcc
     5761 ttgggctcgt cgggcctccg cctcaggacc tcctcgagcg cctcgcgggc ctcgcggccc
     5821 ctgcccaggt tctcgtagag ggaggccagg tggacgcgga gctcgtcgtt ctccggcatg
     5881 atctccacga ggcggacgat ctcctggagg gccgcgtccc agcggccggc cttctcgagg
     5941 agctgctcga tgttgagggc ggcgcggatg aaggaggggt cggcctcgat ggcctcgcgg
     6001 aaccagcgca gggcgtcgtc gacgcggccc tcctcggcgt ggacgaggcc gatgtcgttc
     6061 ctcgcctccg cgttcagggg atcgacggcg aggacctggg tgaagacgtc catggagcgg
     6121 cggtgcttcc ccgtgtgcag gagggcgagg ccgtagcggt tcatcgcctc gacccagccc
     6181 ggcctggcct tcacggcctg ctcgtactcc ttgagcgcct cctcgtgctt gccgagggcg
     6241 tcgtaggcgt agcccaggtt gtagtggaag gaaggatagt tcgggtcgac ctggaggccc
     6301 cgccagaaga ccctgacggc cttctcggga tcgcccatcg cgcggtagac gccgcccagg
     6361 ttgttgtagg ccaggacgtg ggaggggtcg atctcgatgg ccttcgcgta ggacatctcc
     6421 gcggccttga ggttccccgt ctggcggtgc gcgttgccca ggctgaagag gatctccgcg
     6481 ttctcggggg ccgccccgag ggcccgctcc agcgcgccga tggcgtcctt gggcttcccg
     6541 agcttgaggt aggcctcgcc gatcccgatg agggcctcga tgttcccggc gtcccgggcg
     6601 atgacgctcg cgaaggcgga gagggcgtcc tgggcgcggc ccgtccgctg gtagacgccg
     6661 cccaggagga ggcgggcctc gtcggactcc ggccgctcca ggaggaactg cctggccagg
     6721 cgctcggcgc tctcgaagtc gcggagccgc aaggcccctc tcgccctctc gagcagctgg
     6781 gccggattct tcgtcgcgct tgagctcatc gtgcggtcct cgaggggcgg acggagacct
     6841 ccgtggagaa ctcccccgcg cgggatgcgg ctgccgatgc ggaggcggcg gccccggcgg
     6901 tcccggggcc ggcgcccgcc cgggcggccg agtcgtaggc cgcgacggcg acgtagacga
     6961 gccgtccgtt cgcgaggcct gtgagggtca gggtggtgga gaggccggcg tcgacggggc
     7021 tcgggccctc ggccgctccg acgccgaagt actcgccggg ggcgtcgccg tagtagacga
     7081 ggtagccggc gacgtcggac tcgggcacgc gggtccagcg cagctcggcg gctccgtcct
     7141 tcgggaggcc cgcgaggcgg gcgggaggag gaggcggagg gtcgggctcg taggccaccg
     7201 cgatcgagga gagggccggg cccaggcggc ccgtcccgtc ggcgtagagg tcggcgcgga
     7261 cctggaggta gcgcccgcgg gcgcccgcgg gcagggcctc gccggggcgg aagggcgtcc
     7321 aggcggggta gtccaggcgc cagcccgccc agtcctcggc caggcggtag gagagctcga
     7381 cgctggagtt gcccggggtc ctcgcgacgg cctcgatgga ggcgaggcgg ctgtggccga
     7441 agcccaggtc gacgacgggc gagaggacga ggccggggtc gcggccgtag ggcgagaggc
     7501 tcggcttctc cacgaagcgg cgcgagatcc tgagctcgtc cattaggccg gcgtagccct
     7561 cgcccacgac gaggggcgag gcggcgccgg cggcgggggc gaagacggcc ccgccgggcc
     7621 cggcctcgcg gcccgagggc gtggcgtagg cggtcgcctc ggggcggccg tccacgaggt
     7681 actcgaggag gcccgtgtcg ccgtcgaagc ggaggaggtg gtgggaccag gcgcggggca
     7741 cgagggggct cctggcccgc aggtcgaggc gggtcgccgc ctccgccggc ccggccttgc
     7801 ccgcgacggg cctttcgaag aagttctcga agccccagga gacccggccc gcggcgacga
     7861 cgcaggagag gcgctggggg aggacgccgc cgggcagctt ccgcagggac tgccagagga
     7921 ggatgacctc gccgttctcg gcgttgaccg ggtagagcca gaactcgacg gagaagtccc
     7981 ggaagcggga atccttgccg aagagggccc cggagcgggg ccgcagggag acggccgagc
     8041 ccttgccccg gaaggaggcc gagccgccgc ccaggaggga cttgggcccc tcgacggaga
     8101 agccggggcc ggcctcggcc tcgtagttgc cggcctcgtc gcggggagcc ccgccgtcga
     8161 agtggaggag gaggtcggag gcctcgtcgg cccggtaggc ggcgtgggag aggacgtagt
     8221 cgaggccgcc tccgcggcct ggcagggcga cggccccgtc ggcggtccag ctcccgacga
     8281 gggggacccg ggaggccgcg ccggaggcct cgcccgcggc gcccggggcg ccggcccggg
     8341 ccggctggac gggcccgagg cggagggcgc cgccccccgg cgtgagggcg atccgctcca
     8401 ggaaggcctc gccgggattg gccccggcgg ggaccgtcgc ggccgagacg gcggcgagga
     8461 gtatcgcgag gaaggctatt cccttgttgc tcattcgtac gtacattgat tatcgtccac
     8521 tatggccgat aataaagcgg gtaggaagcc ccatcgcgag ggagaagccg ccgattcccc
     8581 ctcgaaggac cggaaagcgg ggctcaaggc cctcgtgcgc cagtcccccc tcgagccggg
     8641 cgtctacatc atgcgcgacg cctcgggctc gatcatctac gtcggcaagg ccaaggtcct
     8701 gaggaaccgg ctttcctcct acttcacggg ccgcaaggac gtgaagaccc gccacctcgt
     8761 ctcccgcatc gagtccatcg agtggatagt ctccccctcc gagtacgagg ccctcctcct
     8821 ggagaacaac ctcatcaagg agaattcgcc caagtacaac atcaacctga aggacggaaa
     8881 gacctacccg agcgtccgcg tcacgaacga gcccttcccg cgggtcttcc gcacgaggcg
     8941 gatcgtcaac gacgggagct cctacttcgg ccccttcccc tcggccgaga ccatcgacac
     9001 ctacctggac ctgatccgcc gcctcttccc cctgcgccgc tgctcggcga tgaagaagcg
     9061 cgaggcgccc tgcatgtact accacatcgg ccgctgctcg gccccctgcg ccgggaagat
     9121 cggcgaggcc gagtacgccc tccacgtcga ggaggtgaag aagctcctct cgggcgagac
     9181 cgaggccctc ctctccgagc tcagggagcg gatggccgag gccgccgccg ccctgcgctt
     9241 cgaggaggcg gggaggctcc gcgacgccgt ccaggccatc gagctcttcg ccggcaggtc
     9301 tggcgccgtc gacttcgacc ccggcgcccg ggactacgtc gcccaggcgg cggacgggga
     9361 cctggtcgtc ttcgcggcct tccagatgcg cggcgggaag ctcaccggcc gcgacgtgta
     9421 ccggagccgc ctctacggcg gcgaggagga ggccctggag gccttcctca tggcctacta
     9481 cggcgg
//
LOCUS       DAOOSJ010000083         9472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4658_length_9472_cov_6.261230, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000083 DAOOSJ010000000
VERSION     DAOOSJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_9472_cov_6.261230"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLG14_05625"
     CDS             complement(<1..797)
                     /locus_tag="PLG14_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOX48649.1"
                     /translation="MAYTVSIAEEKMRNSRKRAYMPIWVYIIVPFAVIVALSVGAVTL
                     SALAAGRTTVRDLARSITWKTAGEIESRVKSYLGTPQLLLGALAETGKQGALDLGNPR
                     AQRGLLYGFAGVEPAVSTFYYGDKEGRTALVSRKPDGSGIFALRDGRTGGNLEIYALD
                     SGGGLGALDSASPFDPRERAWYKAAEAAGKPGWTDIYADFVTGGLVITPYVPLQDGSG
                     GLQGVFGADLPLENLNAIVGEIAKGVGAEALVLDSGGRLVATSTGDPV"
     gene            complement(818..2188)
                     /locus_tag="PLG14_05630"
     CDS             complement(818..2188)
                     /locus_tag="PLG14_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48650.1"
                     /translation="MNRLPRLPALVLAVLTLGAGRLAAAAPPPRPLGGDAVLERMPES
                     AGLRARNWEGFLAAGSETVLAAAPRTAVNEFGAWSLSSVRAKDYFYLIVAPGKGGDFP
                     LYAQGSWIVKRSYPGGRFVQAKVFLRSDPGTFLRLYPDGDRSRMDLVLYGGALAKEVP
                     LPVPFERLLRASVAEIAAWSRDSVDWSLFSPRPGLYADTRRLAEGIRSRLPSLAYRDD
                     GALDAEGRPVLIATGAPQAGAPGLNCSGFAKWVADGLRGPAADGGPPWLDPRAMARRH
                     EAARGSSFTAPFEASQDPFFGLDWTRNLAKALSDARSPSRDHGILEADVRLSPFALYS
                     PSSDPVNGGSPYEDYPPYEENLGFQARGLKALLYVLAAREPGSVYFASLSRADKDGMR
                     RHYHVALLAPYFEASGEFRVAVFESAAETSLEALVARGPKDFVHLVRVRAESSFDPPP
                     FPARRE"
     gene            2291..3349
                     /locus_tag="PLG14_05635"
     CDS             2291..3349
                     /locus_tag="PLG14_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48651.1"
                     /translation="MRMNSAWAPLLACALLLSAAGLAGAEASPGAQGGPLPLLAVPAS
                     AAGLAPAASAASAAPASAAAPSLVVPAGLAGALDVPDEAELARRLSPADEIPVLANNQ
                     VLAFYGHPFSKKMGILGEYSKEELAKLLRGYAKLYDDANGPGLGVVPAFYLIYGTCWP
                     EGEIGYLRESVVKEYIDYAASQGMLVFVDHQIGKYKVEEAVKRLLPFAKYPNVHLALD
                     PEWRTTAPMKEIGSISAEELNKAQAMIDDYLKQEGIPGRKMLVVHQFKGRMIEGRERV
                     RADFGKVILVHTADGFGAPALKRNSYAFNAKAPNMPIKGFKLFFKSGVPGAGYDDPLL
                     TPPEVLALDPMPMLIMYQ"
     gene            3534..7775
                     /locus_tag="PLG14_05640"
     CDS             3534..7775
                     /locus_tag="PLG14_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOX48652.1"
                     /translation="MAGHRTYGAIVALAALLSGAVTAFALDTPAGGYQVPLLASPEAL
                     GYGLTATALDAPWADRLNPAASAGQQRPALSAAFVGLTDFGAAGQGFGGAGSLGLSVP
                     KPFGVLGAGLRLLWTPDSMTDLPLGTLFEARGTIAKDLFPDFYVGAGLGLALGSKGGS
                     DWGLGLDLGFVHLLGDKGFLKDLRWGGVLAGIGKGYEPAGASGAYPPPFTPTLGARAL
                     LLRSADWKLGMGLDLSAPTFQDLGLGLQASVSYKDLVALRSAWGFSVGELAAGNGRSL
                     FPSISLTANIPIERKADESFLAKQGLNKGELRPTLAAAPLYGDVWALGAGVTIPLGVI
                     DRKPPRIDAKFPGQAWPPEPAQTAAAPVSPAPSAAAAPAAPAAPAPGAPLVYHISPNA
                     DGVQDLLEIPVSISDERYLVGYTLTVRKAGAAASEAPLRLIANKESRPETQGLGGLWD
                     RLVYVKKGVPVPDKLVWNGRADDGSTLPDGEYYVLVEAVDDNGNSGRAGPFAVVVDST
                     PPEAKAEAAEASKIFSPDGDGNKDSLLLKVSGSPEDLWKLRILDAAGKAVRSADYSAG
                     APSDFAWDGKADSGEVLPDGVYSMVLSSTDRAGNAFSRRVDNVVVNTQQPPVNVAIDL
                     SAFSPNGDGQKDRVNLAPSVPVRTGLATWKLAVLDKDKKERWSRAGSDSVSLPERLAF
                     EGADLEGKPLPEGQYQAELSVTYVNGHSPKSYSPFFTLDLTPPSATVKADRAAFNPAG
                     REGLNLVRFAQSGSQEERWTGEILGPGGAVARTYSFGSEPDASVEWDGADDAGKPVPD
                     GVYSYRLKAVDRAGNAFSSAPASVALDTEEKAARLSADQRAFSPNGDGQKDVIRFLPQ
                     VQAADRLRSYELVVEAVEAAGLAAGSAVRTWKGEKALPEALAWDGKGDAKAQAADGRY
                     LARLKATYVTGEAVEASTQAFVLDTKAPSLEVKAESLLFSPNGDGRKDSLGFSQKSLP
                     GDDWEGRILAEGGAAGAQPVRSFAWKGQAKDFSWDGADEAGNRVKDGLYRYEVSSVDQ
                     AGNSFKAQVAGIRVDARAVQVFLTASDTGISPNGDGFRDSLSFAPIVNLRDGVEGWRL
                     SVVDKAGKELKSFSGKGGDIPARIDWDGKDASGAVVQGEAAGLLAVDYAKGDRAEAKT
                     ASILVDVDGPKVDVRLTPEFFSPDNDGVDDELSIAISVSDAGETAEWRLEIVEAAVVE
                     GAAPGAKPRERLFIAWGGKGQPAASIDWDGRSAKGELVEAATDYPYYFTIKDELGNVS
                     RKTGMISVDVLVIREGDRLKIKVPSIVFRSGAADFKGLEDEKLANNDKVVKRIAQILN
                     RFRDYRIGIEGHANSEGKITGASQARIAEEETKELIPLSTGRAELVRDLLVKYGVDAK
                     RLSVRGLGSSAPVVDFKDADNRWKNRRVEFILIKN"
     gene            7949..>9472
                     /locus_tag="PLG14_05645"
     CDS             7949..>9472
                     /locus_tag="PLG14_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOX48653.1"
                     /translation="MKELTGRHLYHAFSAGARRVRASQDELNRINVFPVPDGDTGTNM
                     AVTITRAVEGATVSDSASVTMGSMADAALEGGRGNSGVILAQFLAGFREAISDSASVS
                     LERFVRAIEQAYQSSYRAITLPREGTILSVIRDWAAALRREHERSESFKELFSAASPA
                     LRESLAATTGQLDVLKAAGVVDAGASGFVAFIDGIHDYLEAGAPLEGLAADSQGRLEI
                     AETEAELPAGEGRPERRYCAEFLLAAAEGRPLDLDGLRSLLSALGDSVVVAGGPGKAR
                     AHVHTDDPAQAMEELSLRGWVLEQKADDMRRQYEDAHDRAARTAIVTDSACDLPPGLL
                     DAHRVHVAPLYLRFGEREYLDRLTLDPGRFYDLADAAPVFPSSSQPSEAQLARLYRTL
                     LSHYDSVVAIHVSGKLSGTLEASRRAARAVDPARIHVADSRHVSGSLGLIVLRAAEHL
                     EAGGDAASLLALLPEWSRKARNLVSVRSLRYMVRGGRVSPLGGVLAKLLNLKPIVSTD
                     "
BASE COUNT         1398 a         3533 c         3218 g         1323 t
ORIGIN      
        1 acgggatcgc ccgtgctcgt cgcgacgagg cggccgccgg agtcgaggac gagggcctcg
       61 gcgccgacgc ccttcgctat ctcgccgact atggcgttca ggttttcgag gggcaggtcg
      121 gcgccgaaga cgccctggag tccgcccgag ccgtcctgaa gggggacgta gggcgtgatc
      181 acgaggccgc ccgtgacgaa gtccgcgtag atgtccgtcc agcccggctt gcccgcggcc
      241 tccgcggcct tgtaccaggc ccgctcgcgg ggatcgaagg gcgaggccga gtcgagggcc
      301 ccgaggccgc cgcccgagtc gagggcgtag atttccaggt tgccgccggt ccttccgtcg
      361 cggagcgcga agatgcccga gccgtcgggc ttccgcgaga cgagggccgt gcggccctcc
      421 ttgtcgccgt agtagaaggt ggagaccgcg ggctcgacgc cggcgaagcc gtagaggagg
      481 ccgcgctggg cgcgggggtt tcccaggtcg agggctccct gcttgcccgt ctccgccagg
      541 gcgccgagga ggagctgggg ggtgccgagg tagctcttca ccctggactc gatctccccc
      601 gccgtcttcc aggttatgga tcgcgcgagg tcgcgcacgg tcgtccggcc cgccgccagg
      661 gcgctcaggg tcaccgcccc cacggagagg gccacgatga ccgcgaaggg gacgatgatg
      721 tacacccaga tgggcatgta ggctcgcttg cggctgttac gcattttttc ctccgcgatg
      781 gagacagtgt aggccatgtt ccctccggac gccacgctca ttcgcggcgg gcggggaagg
      841 ggggcggatc gaaggaggac tcggcccgga cgcggaccag gtggacgaag tccttcgggc
      901 ccctcgcgac gagggcctcg agactcgtct ccgcggcgct ctcgaagacg gccacgcgga
      961 attcccccga ggcctcgaaa tagggcgcga ggagggcgac gtggtagtgc cgccgcatcc
     1021 cgtccttgtc cgcgcggctc agggacgcga agtagaccga gcccggctcg cgggccgcga
     1081 ggacatagag cagggccttg aggccccgcg cctggaaacc caggttctcc tcgtagggcg
     1141 gatagtcctc ataggggctt ccgccgttca cgggatcgga ggaggggctg tagagggcga
     1201 agggcgagag gcggacgtcg gcctccagga tgccgtggtc gcgcgagggc gagcgggcgt
     1261 cggagagggc cttggcgagg ttccgggtcc agtcgaggcc gaagaaggga tcctggctcg
     1321 cctcgaaggg ggcggtgaag gaggagcccc tggccgcctc gtggcggcgg gccatggccc
     1381 gggggtcgag ccagggagga ccgccgtcgg cggcggggcc gcggaggccg tcggccaccc
     1441 atttcgcgaa gcccgagcag ttgaggccgg gcgctccggc ctggggcgcg ccggtcgcga
     1501 tgaggacggg cctgccctcc gcgtccaggg cgccgtcgtc gcggtaggcg agcgagggca
     1561 ggcgcgagcg gatgccttcc gccaggcggc gcgtgtcggc gtagaggccc ggccggggcg
     1621 agaagaggct ccagtccacg gagtccctgg accaggcggc gatctccgcg acggaggccc
     1681 tgaggaggcg ctcgaagggc acgggcaggg gcacttcctt ggccagggcc ccgccgtaga
     1741 ggacgaggtc catgcggctg cggtcgccgt ccgggtacag tctgaggaag gtgccggggt
     1801 cgctcctgag gaagaccttc gcctggacga agcggccgcc gggataggac cgcttcacga
     1861 tccagctgcc ctgggcgtag agggggaagt cccctccctt cccgggggcg acgatgaggt
     1921 agaagtagtc cttcgcgcgg accgaggaga ggctccaggc gccgaattcg ttcacggccg
     1981 tccgcggggc ggcggccagg acggtctccg agcccgcggc caggaagccc tcccagttcc
     2041 gcgccctgag gcccgcgctc tcgggcatcc gctccaggac ggcatcgccg cccaggggcc
     2101 ggggaggggg agccgcggcg gcgagcctgc ccgctccgag ggtgaggacg gcaaggacga
     2161 gggcggggag tcggggcaac ctgttcatac cactctattg tcggaatcag tcccccgggt
     2221 ctatagggga tgctcccgga ggccgatagg aagagggcaa gcatatacct caccccaagg
     2281 gattctcgcc atgcgaatga actccgcctg ggcgccgctc ctcgcgtgcg cccttctcct
     2341 ctccgccgcg ggcctggccg gggccgaggc ctcgcccggg gcccagggcg gcccccttcc
     2401 ccttctcgcc gtgccggcct cggccgccgg cctcgcgccc gcggcttccg ccgcctcggc
     2461 cgcgcccgct tccgcggccg cgccttccct ggtcgtcccc gccggcctgg ccggcgccct
     2521 ggacgtgccc gacgaggccg agctcgcgcg caggctctcg cccgccgacg agattcccgt
     2581 cctcgcgaac aaccaggtcc tcgccttcta cggccatccc ttctccaaga agatgggcat
     2641 cctgggcgag tactccaagg aggagctcgc gaagctcctc aggggctacg cgaagctcta
     2701 cgacgacgcc aacgggcctg gcctcggcgt cgtgcccgcc ttctacctca tctacggcac
     2761 ctgctggccc gagggggaga tcggctatct ccgcgagtcc gtggtcaagg agtacatcga
     2821 ctacgcggcc tcccagggca tgctggtctt cgtcgaccac cagatcggca agtacaaggt
     2881 cgaggaggcg gtgaagcgcc tcctcccctt cgcgaagtac ccgaacgtcc acctcgccct
     2941 cgatcccgag tggaggacga ccgcccccat gaaggagata ggctccatca gcgccgagga
     3001 gctcaacaag gcccaggcca tgatcgacga ctacctcaag caagagggca ttcccggccg
     3061 caagatgctc gtcgtccacc agttcaaggg caggatgatc gaggggcggg agagggtccg
     3121 cgccgatttc gggaaggtca tcctcgtcca taccgccgac ggcttcggcg ctccggcctt
     3181 gaagcgaaac tcctacgcct tcaacgcgaa ggcccccaac atgccgatca agggcttcaa
     3241 gctcttcttc aagtcgggcg tccccggcgc cggctacgac gatcccctcc tcacgccccc
     3301 cgaggtcctc gccctcgatc ccatgccgat gctcatcatg taccaatgag cccgcttccg
     3361 cggctggcct ccgtcggcca tccgcggtat agtttctgta aggcccaggc ccggtcggga
     3421 cgcgtcgcgc gtaacctttg gcccgaatcc cgccttttcc ttcatagagc ctgcgctata
     3481 ttaaagcaaa gaaagcccgc gcacagagat agacagatcc caggaggaac cgcatggctg
     3541 gtcataggac ttatggcgcg atagtcgccc tcgccgccct cttgtcgggc gctgtcacgg
     3601 cctttgcctt ggacacgccg gctggcggct accaggtgcc gctcctcgcc tctcccgagg
     3661 ccctgggcta cggcctcacg gccacggccc tggacgctcc ctgggccgac cgcctgaacc
     3721 ccgcggcctc cgcgggccag cagcgccccg ccctctccgc ggccttcgtc ggcctcaccg
     3781 acttcggcgc cgccggccag ggcttcggcg gggcgggaag cctgggcctt tcggtcccga
     3841 agcccttcgg cgtcctgggc gccggcctgc gcctcctttg gacgccggac tcgatgaccg
     3901 acctccccct cggcaccctc ttcgaggccc gcgggacgat cgcgaaggac ctcttccccg
     3961 acttctacgt cggcgccgga ctcggcctgg ccctgggctc caagggcggc tcggactggg
     4021 gcctgggcct ggacctgggc ttcgtccatc tcctgggcga caagggtttc ctcaaggacc
     4081 tccgctgggg cggcgtcctg gccggcatag gcaagggcta cgagcccgcc ggagcctcgg
     4141 gggcctaccc tccgcctttc accccgaccc tgggcgcgcg ggccctcctc ctccgctccg
     4201 ccgactggaa gctcggcatg ggcctcgacc tctccgcccc caccttccag gacctgggcc
     4261 tgggcctcca ggcgagcgtc tcctacaagg acctcgtggc cctgcggagc gcctggggct
     4321 tctccgtcgg cgagctcgcg gctggtaacg ggcgcagcct cttcccctct atctccctca
     4381 ccgcgaacat cccgatcgag cgcaaggccg acgaatcctt cctggccaag caggggctca
     4441 acaagggcga gctcaggccg accctggcgg ccgcgccgct ctacggcgac gtctgggccc
     4501 tgggcgcggg cgtcaccatc cccctcggcg tgatcgaccg gaagcctccc cgcatcgacg
     4561 cgaagttccc cggccaggcc tggccgcccg agccggccca gactgccgcc gctcccgtct
     4621 ccccggcccc ctccgcggcc gccgcgcccg cggctcccgc cgcgccggct ccgggagccc
     4681 ccctcgtcta ccacatctcg cccaacgccg acggcgtcca ggacctcctc gagatcccgg
     4741 tctccatctc cgacgagcgc tacctcgtgg gctacaccct caccgtgcgc aaggccggcg
     4801 cggccgcctc cgaggcgccg ctcaggctca tcgcgaacaa ggagagccgc cccgagaccc
     4861 agggcctggg aggcctctgg gacaggctcg tctacgtgaa gaagggcgtg ccggtgccgg
     4921 acaagctcgt ctggaacggc agggccgacg acggctccac cctccccgac ggcgagtact
     4981 acgtcctcgt cgaggcggtg gacgacaacg gcaactccgg ccgcgcgggc cccttcgccg
     5041 tcgtggtcga ctccaccccg cccgaggcca aggccgaggc ggccgaggcc tccaagatct
     5101 tcagccccga cggcgacggc aacaaggaca gcctcctcct caaggtctcc ggcagccccg
     5161 aggacctgtg gaagctcagg atcctggacg cggccggcaa ggctgtgcgg agcgccgact
     5221 actcggccgg cgccccctcc gacttcgcct gggacggcaa ggccgactcc ggcgaggtcc
     5281 tccccgacgg cgtctattcc atggtcctct cctcgaccga cagggcgggc aacgccttct
     5341 cgcggcgggt ggacaacgtc gtcgtcaaca cccagcagcc ccccgtcaac gtcgccatcg
     5401 acctctccgc cttcagtccc aacggagacg gccagaagga cagggtcaac ctggccccga
     5461 gcgtgcccgt ccggacgggc ctcgcgacct ggaagctcgc cgtcctcgac aaggacaaga
     5521 aggagcgctg gtccagggcc ggctccgact ccgtctccct ccccgagcgc ctggccttcg
     5581 aaggcgccga cctggagggc aagcccctcc ccgagggcca gtaccaggcc gagctctccg
     5641 tcacctacgt gaacggccac agccccaagt cctactcgcc cttcttcacc ctggacctca
     5701 cgccgccctc cgcgaccgtg aaggccgaca gggccgcctt caacccggcg ggccgagagg
     5761 gcctgaacct ggtccgcttc gcccagtccg gctcccagga ggagcgctgg accggcgaga
     5821 tcctgggccc gggcggcgcg gtcgcgcgga cctactcctt cggctccgag cccgacgcga
     5881 gcgtcgagtg ggacggagcc gacgacgccg gcaagcccgt gcccgacggc gtttacagct
     5941 accggctcaa ggccgtggac cgggcgggca acgccttctc ctccgccccg gcctccgtcg
     6001 ccctcgacac cgaggagaag gcggcccggc tctcggccga ccagcgcgcc ttcagcccca
     6061 acggcgacgg ccagaaggac gtcatccgct tcctccccca ggtccaggcg gccgaccgcc
     6121 tcaggtccta cgagctcgtg gtggaggccg tggaggccgc cggcctagcc gcgggctccg
     6181 cggtcaggac ctggaagggc gagaaggccc ttcccgaggc cctggcctgg gacggcaagg
     6241 gcgacgcgaa ggcccaggcc gcggacgggc gctacctggc ccgcctcaag gcgacctacg
     6301 tcaccggcga ggcggtggag gcctccaccc aggccttcgt cctcgacacc aaggccccct
     6361 ccctcgaggt caaggccgag agcctcctct tctcccccaa cggcgacggc cgcaaggact
     6421 cgctcggctt cagccagaag tcgcttcccg gcgacgactg ggagggacgg atcctcgccg
     6481 agggcggcgc cgcgggcgcg cagcccgtcc ggagcttcgc ctggaagggc caggccaagg
     6541 acttctcctg ggacggcgcc gacgaggcgg gcaaccgcgt caaggacggc ctctaccgct
     6601 acgaagtctc ctccgtcgac caggcgggca actccttcaa ggcccaggtc gcgggcatcc
     6661 gcgtcgacgc ccgcgcggtc caggtcttcc tcaccgcctc cgacacgggc atctccccga
     6721 acggcgacgg cttccgcgac tccctctcct tcgctcccat cgtgaacctg cgcgacggcg
     6781 tcgagggctg gaggctctcc gtcgtcgaca aggcgggcaa ggagctcaag tccttctccg
     6841 ggaagggcgg ggacatcccg gccaggatcg actgggacgg caaggacgcc tccggcgccg
     6901 tcgtccaggg agaggcggcg ggcctcctcg cggtcgacta cgcgaagggc gacagggccg
     6961 aggcgaagac cgcctccatc ctcgtcgacg tcgacgggcc gaaggtcgac gtgcgcctca
     7021 cgcccgagtt cttcagcccg gacaacgacg gcgtcgacga cgagctctcc atcgccatct
     7081 ccgtctccga cgcgggcgag acggccgagt ggcgcctcga gatcgtcgag gcggcggtcg
     7141 tggagggcgc ggcgcccggc gccaagccca gggagcggct cttcatcgcc tggggcggca
     7201 agggccagcc cgccgcctcc atcgactggg acggccgctc cgcgaagggc gagctcgtcg
     7261 aggccgcgac cgactatccc tactacttca cgatcaagga cgagctcggc aacgtgtcca
     7321 ggaagacggg catgatctcc gtcgacgtcc tcgtgatccg cgagggcgac cgcctcaaga
     7381 tcaaggtgcc ctccatcgtg ttccgctccg gcgccgccga cttcaagggg cttgaggacg
     7441 agaagctcgc gaacaacgac aaggtggtga agcgcatcgc gcagatcctg aaccgcttca
     7501 gggactaccg gatcggcatc gagggccacg cgaacagcga gggcaagatc acgggcgcct
     7561 cccaggcgcg gatcgccgag gaggagacga aggagctgat ccccctctcc accggtcgcg
     7621 ccgagctcgt ccgcgacctc ctcgtgaagt acggcgtgga cgctaagcgc ctctcggtgc
     7681 ggggcctcgg ctccagcgcc cccgtcgtcg acttcaagga cgcggacaac cgctggaaga
     7741 accgccgcgt ggagttcatc ctcatcaaga actagcccgc cttcgcgcga gtccagggcc
     7801 gccgcggcga cgcggcggcc cttttttcgc cgcgaggcca tggtcccgcc tcgcgcggcc
     7861 ccgccccggc ggcggaacct cccttgcttt ttcgtacggc gagagtattt tactctatgc
     7921 cgggccagcg gccggcgggg gagcagcatt gaaggaactc accggtcgcc acctctacca
     7981 cgccttcagc gccggcgcgc gccgggtgcg cgccagccag gacgagctca accgcatcaa
     8041 cgtcttcccc gtgcccgacg gcgacacggg cacgaacatg gccgtgacca tcacgagggc
     8101 cgtcgagggc gcgacggtct ccgactcggc ctccgtgacg atgggctcca tggccgacgc
     8161 cgcgctggag ggcggccgcg ggaactcggg cgtcatcctg gcgcaattcc tcgcgggctt
     8221 ccgcgaggcc atctccgact cggcctcggt gagcctggag cgcttcgtcc gggccatcga
     8281 gcaggcctac cagagctcct accgggccat caccctgccc cgggagggca ccatcctctc
     8341 cgtgatcagg gactgggccg cggcgctcag gcgggagcac gagaggtcgg agtccttcaa
     8401 ggagctcttc agcgcggcct cgccggccct ccgcgagtcc ctggccgcga cgacgggcca
     8461 gctcgacgtc ctcaaggcgg cgggcgtcgt ggacgcgggg gcctcgggct tcgtcgcctt
     8521 catcgacggc atccacgact acctggaggc cggcgccccc ttggaggggc tggcggcgga
     8581 cagccagggc cgcctggaga tcgccgagac cgaggccgag ctgcccgcgg gcgaaggccg
     8641 tcccgagcgc cgctactgcg cggaattcct cctcgccgcc gcggaagggc gccccctcga
     8701 cctcgacggg ctccgaagcc tcctctccgc cctgggggac tccgtcgtcg tcgcgggcgg
     8761 ccccgggaag gcccgcgccc acgtccacac ggacgatccg gcgcaggcga tggaggagct
     8821 ctccctgcgg ggctgggtcc tggagcagaa ggcggacgac atgcggcgcc agtacgagga
     8881 cgcgcacgac cgagccgcca ggacggccat cgtcacggac tcggcctgcg acctgccgcc
     8941 cgggctcctg gacgcgcacc gcgtccacgt cgcgccgctc tacctccgct tcggcgagcg
     9001 ggagtacctg gaccgcctga ccctcgatcc cgggcgcttc tacgacctcg cggacgcggc
     9061 tcccgtcttc ccctcctcct cccagccctc ggaggcccag ctcgcgcggc tctaccggac
     9121 cctcctctcc cactacgact ccgtcgtcgc gatccacgtc tcgggaaagc tctcgggcac
     9181 cctggaggcc tcgaggcgcg cggcccgggc cgtcgatccc gcgcggatcc acgtcgccga
     9241 ctcgcggcac gtctccggct ccctcggcct gatcgtcctc cgcgcggccg agcacctgga
     9301 agccggcggc gacgccgcga gcctcctcgc gctgctcccc gagtggtcgc gcaaggcccg
     9361 gaacctcgtg agcgttagga gcctcaggta catggtcagg ggcggcaggg tgagccccct
     9421 gggcggcgtc ctcgcgaagc tcctcaacct gaagcccatc gtctccaccg ac
//
LOCUS       DAOOSJ010000084         9454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4671_length_9454_cov_7.022024, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000084 DAOOSJ010000000
VERSION     DAOOSJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_9454_cov_7.022024"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLG14_05650"
     CDS             complement(<1..413)
                     /locus_tag="PLG14_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48654.1"
                     /translation="MSDDIILEVQGLKTEFRAEYGLVKAVDGVSFELRRGETLGVVGE
                     SGSGKSVTNLSILRLVPSPPGRTAGGKVLFRAVAGGPEIDLLEAPEEEVRVLRGKSIS
                     MIFQDPLTSLNPVLRVSLQIGEALQLHHGLSRREA"
     gene            complement(422..1258)
                     /locus_tag="PLG14_05655"
     CDS             complement(422..1258)
                     /locus_tag="PLG14_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48655.1"
                     /translation="METRALRKNSIALTGLAIVGFYVAAILLSLVWLPFDPVAMDTEH
                     LLEGPSLATGHLFGTDEFGRDILSRIMKGASVSLVISICATALGAVVGVLMGIWAGYL
                     GGRWDQFLMRIADILFSFPSLLLAIFVMAILGEKTYNVVFAIGIVYIPQFARVSRGAI
                     LTIKSSEFVRAARSNGASPRFILFRHLLPNILVPLIVQVSLSLSVAILLESSLSFLGL
                     GVQPPYPSWGNMLSAGRKVMVIAPWTAVYPGLAIVFLVLGFNLLGDGLRDILDPRLKN
                     AR"
     gene            complement(1262..2224)
                     /locus_tag="PLG14_05660"
     CDS             complement(1262..2224)
                     /locus_tag="PLG14_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48656.1"
                     /translation="MGRYIAKRLLSLIPVLFGVSVIVFFLVRLIPGSALQMYMGTQVE
                     ATPEQMEELRRIFGEDRPVPVLYVEWLGRLLQGNFGYSLRTGRPVLPDIVSRLPLSLE
                     LTVLALFLALLIGIPQGILSSLRKNPFWDVVNRIVGLLGLSLPQFWLAALMVLAFSGF
                     RGWIPMGNYAKFTEEPLRNLKMLLLPSLAIGIGLAAVIMRYTRNSMLEVLQADYIRTA
                     KAKGLKKRVVILRHALKNAILPVITVAGFNTGYLLGGAIVIEEVFALPGMGRLALYAI
                     YQRDYPVIQGIVLVIATLFVLVNLVTDLIYALVDPRIRLDDSSR"
     gene            complement(2372..3889)
                     /locus_tag="PLG14_05665"
     CDS             complement(2372..3889)
                     /locus_tag="PLG14_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX48657.1"
                     /translation="MKRLSILLLALALLALSGAAAFAAPAEKVLTIGVDQEAVGLDPH
                     VVTAFSSMRRIDLLYNRLVRLDDNFVVVPDLAQSWDIPDNLTYVFHLRKGVKFHNGRE
                     MTAEDVQYSLNRVLDPKTASPGRSYISLVKAIDVVDRYTVKLTLSAPLASLLDGLTSN
                     NLSIVPKEVVEAQGNLQKVEAGTGPFMLKEWIADNSMTLVKNPDYFEKGAPALDKVIF
                     RVIPEQASLLAGVKSGDLDMATINDGATIRQAAKDPKVVVMSKPGLNVRIFSFNTTRK
                     PFDDVRVRAAFAQVLDRSEILAIAEFGMGKPTGPIPVAATQWATPVPKLPFAKPDIAA
                     AKKLLAEAGYPSGFSFKITCSSTYEGGLAVAQVVQDELKKIGVTAELEVVEWGVYIDK
                     WVKRDFDTMVELRGGSGEPDRFLYRTFHSTGGVNNFLFKDAEVDKYLDLGRNQFKPAD
                     RKASYDKLQALLIEKAPAVFLYCPNENHVLSPKVTGFRQVGNGSLYYITAAQVKR"
     gene            complement(3931..5163)
                     /locus_tag="PLG14_05670"
     CDS             complement(3931..5163)
                     /locus_tag="PLG14_05670"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOX48658.1"
                     /translation="MEKRSDEVGFRAAYAAKAEHLVIGIMSGTSLDGVDAVLLRVRTE
                     GSGAASGVELEKHVYLPYSDELRARVDALCSPKTARIDELTYVHFGLSEWYAEAVRLL
                     LAEAGKRPSEVDAISMHGQTVWHAPVALPFPGPRGPLPVKGTLQLGSSPVLRERTGIP
                     VIADLRSRDMAAGGEGAPLAPYIDSILFGSRTEGRIVQNIGGIGNATVVPAGAALADL
                     LAFDTGPGNMVMDAVAAAGSGGKLRYDPDGSVAAKGRPSEELVARLMRDPYFARKPPK
                     STGREVYGAAFAAAFMKDCAALGLSFEDSVATATAFTAESIAAAYRDFILPSCPIARV
                     LVAGGGALNPTLLRMIQERLPGSVKVQTTGALGVPDQAREAMAFALMGHESLMGRPAN
                     LPAVTGARGPVVLGTITL"
     gene            complement(5333..6760)
                     /locus_tag="PLG14_05675"
     CDS             complement(5333..6760)
                     /locus_tag="PLG14_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOX48659.1"
                     /translation="MSKGQYNRVEPADAAALEAIVGRRFLIYRDEERLEPYSRDETAE
                     RRYASMPEAVVKPASTEEVSRIMAYACSRRLPVTPRGAGSGLSGGCVPAAGGIVLSLE
                     RMNAILEIDLENLMVVAQPGVVTNSINEALKKDGLMFAGYPMSLETCFLGGNVAENAG
                     GGKAVKYGVTSRYVTGLEAVLPSGQVLRLGGKLRKDVTGYDLIHLLVGSEGTLAVVTE
                     ITLSLLPVPRRRAVLLASFAGAAEAIGAVPRIFREARIVPTSVEYMDRASILASCGYL
                     NESIPYGEAGAMLLVEVEGEGPDEVYANYSAIGAVCEAAGAGRVYVADNRTTMERIWN
                     VRRNIAEALKVRSPRQSLEDIVVPTAAIPAFMEGLLSLAAEEGVEIPCYGHAGDGNLH
                     ATVVKPEAWSEAEWEERLHRVLERLYRLSASLGGRISGEHGIGSKRREYLGLNLDPAT
                     IAIMKGIKAVFDPEGILNPGKIFPD"
     gene            complement(6769..7806)
                     /locus_tag="PLG14_05680"
     CDS             complement(6769..7806)
                     /locus_tag="PLG14_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX48660.1"
                     /translation="MNEVWILAETKAGKVAPVSYELLSWARSLGGPDEVAVTAVLAGP
                     ASDPEELCYRGADAVIHGAEPGLAGFSARPLSAWLASLFGLRRPTVLLAAATATGRTV
                     LPYLAASEGLGLTADCTELSLDPESGVLLQTRPAAGGNIMATIRTARGLPQMATVRPH
                     SRKALDRDPSRPVRVERFAAAAGPGGAGGAGGGQGRAGEGSAGDGLVLLGERPFESSR
                     GLADARLVFAGGKGFRKKEGFELLFRLAELAGGEVGASREAVDRGWADYPRQVGLSGR
                     TISPELYLAFGISGAIQHLAGMQTSGFIVSVNSDPDAPIAAASDLAVRADVYELVPAL
                     AARLEGYRPAE"
     gene            complement(7803..8663)
                     /locus_tag="PLG14_05685"
     CDS             complement(7803..8663)
                     /locus_tag="PLG14_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX48661.1"
                     /translation="MKLAVLVKQVPETDSLRLDPETGTVIRSEDTSIVNPLDLYALEA
                     ALRLKDEDPGVSILALSMGPPQAESALREALAMGCDEAVLVTGREFGGADTWATSRAL
                     AAALGLLGPFDLVFCGEKATDGDTGQVGPEVAAFLGLPVVSYVGSFALDRGSAADRAG
                     SAGAGRLRAERVLEDGIQTVSAPLPAVLTLCKALGEPRLPLLSGKRRAREASVRRLGL
                     AELGLQAEEVGAKGSPTRVVKIDRPRIARRTEVLDARTEEGIEAACDRIVAILLEKGL
                     IVPKEGRAGA"
     gene            8899..>9454
                     /locus_tag="PLG14_05690"
     CDS             8899..>9454
                     /locus_tag="PLG14_05690"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOX48662.1"
                     /translation="MIDVTITQEMLDGFTTESRNERSMDIDKAGSREILAIINEEDKT
                     VPFAVERELDHIAALVDDVVAAFRGGGRLFYIGAGTSGRLGVLDASECPPTYGVPPTM
                     VQGLIAGGLPALVRSIEGAEDDLEAGAKELEAAGFGPGDVLVGITASGQAPYVLGAMA
                     KAKAAGAVVAAISCNKGSKTFQYAK"
BASE COUNT         1465 a         3010 c         3590 g         1389 t
ORIGIN      
        1 ctggcctcgc gcctggagag gccgtggtgg agctgcaggg cctcgccgat ctggagggag
       61 acgcggagca cgggattgag ggaggtgagg gggtcctgga agatcatgga gatgctcttc
      121 ccgcgcagga ccctcacctc ctcctccggc gcctcgagga ggtcgatctc cgggccgcct
      181 gcgacggcgc ggaagaggac cttgccgccc gcagtcctcc ctggcgggct cgggacgagc
      241 ctcaggatcg agaggtttgt cacggacttg cccgagccgg actcgccgac gacgcccagg
      301 gtctcgccgc ggcggagctc gaaggagacg ccgtccacgg ccttcacgag gccgtactcg
      361 gcgcggaatt cggtcttgag gccctggacc tcgaggatga tgtcgtcgct catgatgccg
      421 cctatctcgc gttcttgagg cgcgggtcga gtatgtcgcg gagcccgtcc ccgaggaggt
      481 tgaagcccag gacgaggaag acgatcgcga gccccggata caccgcggtc cagggggcga
      541 tgaccatgac cttgcggccg gcggagagca tgttgcccca ggagggatag gggggctgga
      601 cgcccaggcc gaggaagctc agggaggact cgaggaggat ggccaccgag agggagaggg
      661 agacctgcac gatgagcggc acgaggatgt tcggcaggag gtggcggaag aggatgaagc
      721 gcgggctcgc gccgttggag cgcgccgcgc gcacgaactc gctcgacttg atcgtcagga
      781 tcgcgccccg gctcacccgc gcgaactgcg ggatgtagac gatgccgatc gcgaagacga
      841 cgttgtaggt cttctcgccc aggatcgcca tcacgaagat ggcgaggagg agggagggga
      901 aggagaagag gatgtcggcg atccgcatga ggaattggtc ccagcggccg cccaggtagc
      961 cggcccagat ccccatgagg acgccgacga cggcgcccag ggccgtggcg cagatcgaga
     1021 tgacgaggga gaccgaggcg cccttcatga tgcgcgacag gatgtcgcgg ccgaactcgt
     1081 cggtgccgaa gaggtggccc gtcgcgaggg agggcccctc caggaggtgc tccgtgtcca
     1141 tggcgacggg gtcgaagggc agccagacga gggagaggag gatcgcggcg acgtagaagc
     1201 cgacgatggc gagtccggtg agggcgatgc tgttcttgcg gagggcgcgc gtttccatgg
     1261 cctacctcga ggagtcgtcg agcctgatgc gcgggtcgac gagggcgtag atgaggtccg
     1321 tgacgaggtt cacgaggacg aagagggtcg cgatcacgag gacgatgccc tggatgacgg
     1381 gatagtcccg ctggtagatc gcgtagaggg cgagccttcc catgccgggg agggcgaaga
     1441 cctcctcgat gacgatggcg ccgcccagga ggtagccggt attgaagccc gccacggtga
     1501 tgacggggag gatggcgttc ttgagggcgt ggcggaggat caccacgcgc ttcttgaggc
     1561 ccttggcctt ggcggtccgg atgtagtccg cctgcaggac ctcgagcatg ctgttgcgcg
     1621 tgtagcgcat gatgacggcc gcgaggccga tgccgatcgc gagggagggg aggaggagca
     1681 tcttgaggtt gcgcagcggc tcctccgtga acttggcgta gttgcccatg gggatccagc
     1741 cccggaagcc cgagaaggcc aggaccatga gggccgcgag ccagaactgc ggcagggaga
     1801 gacccaggag gccgacgatc ctgttcacca cgtcccagaa ggggttcttc ctcagggagg
     1861 agaggatgcc ctgggggatg ccgatgagga gggcgaggaa gagggcgagg acggtgagct
     1921 cgaggctcaa ggggaggcgc gagacgatgt ccgggaggac gggcctgccc gtgcgcaggg
     1981 aatagccgaa gttgccctgg aggaggcggc cgagccactc gacgtagagg accggcaccg
     2041 gcctgtcctc gccgaagatg cgcctgagct cctccatctg ctcgggcgtc gcctccacct
     2101 gggtgcccat gtacatctgc agggccgagc ccgggatgag gcggacgagg aagaacacga
     2161 tgaccgagac cccgaagagg acgggaatca gggataagag cctcttggcg atgtatctgc
     2221 ccacggcctg ctccggaaat agggaattgg gatcggggag gggaagctcc gcgcctctcc
     2281 gccccggggg cggagaggcg tggaaggggg gcgccgggga aggcccgcgg gctccgcgcc
     2341 gcgcgggcct tccccaaggc cgcccggaac gctagcgctt cacctgcgcc gcggtgatgt
     2401 agtagaggct gccgttgccc acctgcctga agcccgtgac cttgggcgag aggacgtggt
     2461 tctcgttcgg gcagtagagg aagacggcgg gcgccttctc gatgaggagg gcctggagct
     2521 tgtcgtagga ggccttcctg tcggcgggct tgaactggtt gcgcccgagg tcaaggtact
     2581 tgtcgacctc ggcgtccttg aagaggaagt tgttgacgcc gccggtcgag tggaaggtgc
     2641 ggtagaggaa gcggtcgggc tcgcccgagc cgccgcggag ctcgaccatc gtgtcgaagt
     2701 cgcgcttcac ccacttgtcg atgtagacgc cccactcgac gacctcgagc tcggcggtga
     2761 cgccgatctt cttgagctcg tcctggacga cctgggcgac ggccaggccg ccctcgtagg
     2821 tggaggagca ggtgatcttg aagctgaagc cgctcgggta gccggcctcg gcgaggagct
     2881 tcttcgccgc ggcgatgtcg ggcttggcga aggggagctt cgggaccggg gtcgcccact
     2941 gcgtcgccgc gacggggatg gggccggtgg gcttgcccat gccgaactcg gcgatggcga
     3001 ggatctcgct ccggtccagg acctgggcga aggccgcgcg gacgcggacg tcgtcgaagg
     3061 gcttgcgcgt ggtgttgaag ctgaagatgc ggacgttgag gccgggcttg gacatcacga
     3121 cgaccttggg gtccttcgcg gcctggcgga tcgtggcgcc gtcgttgatc gtcgccatgt
     3181 ccaggtcgcc ggacttcacg ccggcgagga gggaggcctg ctcggggatg acgcggaaga
     3241 tgaccttgtc gagggcggga gcgcccttct cgaagtagtc ggggttcttg acgagggtca
     3301 tcgagttgtc ggcgatccac tccttgagca tgaagggtcc cgtgcccgcc tcgaccttct
     3361 gcaggttgcc ctgggcctcg acgacctcct tcgggacgat ggagaggttg ttcgaggtga
     3421 ggccgtccag gagggaggcg aggggcgcgg agagggtcag cttcaccgtg tagcggtcca
     3481 cgacgtcgat ggccttcacg agggagatgt aggacctgcc ggggctcgcg gtcttcgggt
     3541 cgaggacgcg gttcagggag tactggacgt cctccgccgt catctcgcgg ccgttgtgga
     3601 acttgacgcc cttccggagg tggaagacgt aggtgaggtt gtccgggatg tcccaggact
     3661 gcgcgaggtc gggcacgacg acgaagttgt cgtcgaggcg cacgaggcgg ttgtagagca
     3721 ggtcgatgcg gcgcatcgag gagaaggcgg tcacgacgtg cgggtcgagt ccgacggcct
     3781 cctggtccac gccgatggtg agcaccttct ccgccggggc ggcgaaggcg gcggcgcccg
     3841 agagggcgag cagcgcgagg gcgagcagca ggatcgagag tctcttcatc gaaagcctcc
     3901 tagtctgtac gtctattgtc gaaagccgga tcacagcgtg atggtgccga ggacgacggg
     3961 cccgcgggcc cccgtgacgg cgggaaggtt cgcgggcctc cccatgagcg actcgtggcc
     4021 catgagggcg aaggccatcg cctcccgggc ctggtcgggc acgcccaggg cccccgtcgt
     4081 ctggaccttg acgctcccgg gaagccgctc ctggatcatg cggaggaggg tcgggttgag
     4141 ggcgccgcct cccgcgacca ggacgcgggc tatggggcag gaggggagga tgaagtcgcg
     4201 gtaggcggcg gcgatggact cggcggtgaa ggccgtcgcg gtcgcgaccg agtcctcgaa
     4261 ggagagaccg agggccgcgc agtccttcat gaaggcggcg gcgaaggccg cgccgtagac
     4321 ctcgcgcccc gtgctcttcg ggggcttccg cgcgaagtag ggatcccgca tgaggcgggc
     4381 gacgagctcc tccgagggcc tgcccttcgc ggccacggag ccgtcgggat cgtagcgcag
     4441 cttcccgccc gagcccgcgg cggcgacggc gtccatcacc atgttgccgg ggcccgtgtc
     4501 gaaggcgagg aggtcggcga gggcggcgcc ggccgggacg accgtggcgt tgccgatgcc
     4561 gccgatgttc tggacgatgc gcccctccgt cctggagccg aagaggatgg agtcgatgta
     4621 gggggcgagg ggggcgccct cccctcccgc ggccatgtcg cgggaccgca ggtcggcgat
     4681 gacgggaatg cccgtccgct cgcgcaggac ggggctcgag ccgagctgga gggtgccctt
     4741 cacgggaagg gggcccctcg gccccggaaa gggaagggcg acgggcgcgt gccagacggt
     4801 ctggccgtgc atggagatgg cgtcgacctc ggaaggccgc ttcccggcct cggcgagaag
     4861 gagtcggacg gcctcggcgt accactcgga gaggccgaag tggacatagg tgagctcgtc
     4921 gatccgcgcg gtcttgggcg aacagagggc gtccacgcgg gcgcggagct cgtccgaata
     4981 gggaagatag acgtgcttct cgagctcgac gccggaggcg gcgcccgagc cctccgtgcg
     5041 gacgcgcagg aggacggcgt ccacgccgtc gagcgaggtg cccgacatga tgccgatgac
     5101 gaggtgttcc gccttcgccg cgtaggccgc gcggaacccg acctcgtccg atcgcttctc
     5161 catgctcatc cgccttcctt catgcgccgc ttgggcccgc ttcgatccgg actgggatca
     5221 ttatatggcg cattatttca gtccgcaatg tctatttaaa agattttttc gaccccgcgc
     5281 cgcccagccc tgccgactcg actcgccgcc gcgaggccgc gcgcggcggc cctcaatcgg
     5341 ggaagatctt ccccgggttg aggatgccct cgggatcgaa gacggccttg atgcccttca
     5401 tgatggcgat ggtcgcgggg tcgaggttca ggcccaggta ctcgcgccgc ttgctcccga
     5461 tgccgtgctc ccccgagatc cggccgccca gggaggcgga gaggcggtag agccgctcga
     5521 ggacgcggtg gagccgttcc tcccactcgg cctcgctcca ggcctcgggc ttcacgacgg
     5581 tcgcgtggag gttgccgtcg ccggcgtggc cgtagcaggg gatctcgacg ccctcctccg
     5641 ccgcgaggga gaggaggccc tccatgaagg cgggtatggc ggccgtgggg acgacgatgt
     5701 cctcgaggct ctgcctgggg ctcctcacct tcagggcctc cgcgatgttc cgcctgacgt
     5761 tccagatccg ctccatcgtc gtgcggttgt cggcgacgta gacgcggccc gcgcccgcgg
     5821 cctcgcagac cgcgccgatg gccgagtagt tcgcgtagac ctcgtcgggc ccttcgccct
     5881 ccacctcgac gaggagcatg gcgccggcct cgccgtaggg gatgctctcg ttcaggtagc
     5941 cgcaggaggc gaggatcgag gccctgtcca tgtactccac gctcgtgggc acgatgcgcg
     6001 cctcgcggaa gatgcgcggc acggcgccga tggcctccgc ggcgccggcg aaggaggcga
     6061 ggaggacggc cctcctcctc ggcacgggaa ggagactcaa ggtgatctcc gtgacgacgg
     6121 cgagggtgcc ctcggagccg acgaggaggt ggatgaggtc gtagcccgtc acgtccttcc
     6181 tgagcttccc gcccaggcgc aggacctggc ccgagggcag gacggcctcg agtcctgtca
     6241 cgtaccgcga ggtgacgccg tacttcaccg ccttgccgcc gcccgcgttc tccgcgacgt
     6301 tgccgcccag gaagcaggtc tcgaggctca tcgggtagcc cgcgaacatg aggccgtcct
     6361 tcttcagggc ctcgttgatg gagttggtga cgacgcccgg ctgggcgacg accatgaggt
     6421 tctcgaggtc gatctcgagg atggcgttca tccgctccag ggagaggacg atgccgcccg
     6481 ccgcgggcac gcagccgccc gagaggccgg agccggcgcc gcggggcgtc acggggaggc
     6541 gccgcgagca cgcgtaggcc atgatcctcg acacctcctc cgtggaggcg ggcttcacga
     6601 cggcctcggg catggaggcg tagcgccgct ccgccgtctc gtcgcgggag tagggctcga
     6661 gccgctcctc gtcgcggtag atgaggaagc gccggccgac gatggcctcg agggccgccg
     6721 cgtcggcggg ctcgaccctg ttgtactgtc ccttgctcat ggctcctcct attccgcggg
     6781 cctgtagccc tcgagcctgg ccgcgagggc cgggacgagc tcgtagacgt cggcgcggac
     6841 cgcgaggtcg gaggccgcgg cgatgggcgc gtcggggtcc gagttgacgg acacgatgaa
     6901 gccggaggtc tgcatgcccg cgaggtgctg gatggccccg gagatgccga aggccaggta
     6961 gagttcgggg gagatggtcc ggcccgagag gccgacctgg cggggatagt cggcccagcc
     7021 gcggtcgacc gcttcgcggc tcgcccccac ctcgccgcct gcgagctcag cgaggcggaa
     7081 gaggagctcg aagccctcct tcttccggaa gcccttgccg cccgcgaaga cgagccgcgc
     7141 gtcggcgagg ccgcgggagc tctcgaaggg ccgctccccg aggagtacga ggccgtcgcc
     7201 cgcggagcct tcgcccgcgc ggccctggcc gccgcctgcg ccaccagcgc cgcccggacc
     7261 cgcggccgcg gcgaagcgct cgacgcggac cgggcgggag gggtcgcggt ccagggcttt
     7321 gcgcgagtgg ggacgcacgg tcgccatctg gggcaggccc ctggccgtgc ggatcgtcgc
     7381 catgatgttg ccgcccgccg cgggccttgt ctggaggagg acgccgcttt cgggatcgag
     7441 ggagagctcc gtgcagtccg ccgtgaggcc caggccctcc gaggcggcca gatagggaag
     7501 gacggtcctt cccgtcgccg tcgccgcggc gaggaggacg gtcggccgcc tgaggccgaa
     7561 aagcgaggcg agccacgcgg acagggggcg ggcggagaag cccgcgagcc ccggctccgc
     7621 gccgtggatc accgcgtcgg ccccgcggta gcagagctcc tcgggatcgg aggcgggccc
     7681 cgcgaggacg gcggtgaccg cgacctcgtc cggcccgccc aggctccgcg cccaggagag
     7741 gagctcgtag gagacgggag ctaccttccc ggccttggtc tcggcgagta tccagacctc
     7801 gttcatgccc ctgccctccc ctctttcggt acgatgagcc ccttctcgag gaggatggcg
     7861 acgatcctgt cgcaggccgc ctcgatcccc tcctcggtcc tcgcgtccag gacctccgtc
     7921 cgccgcgcga tccgcggccg gtcgatcttc acgacccgcg tcggcgagcc cttggcgccg
     7981 acttcctccg cctggaggcc gagctcggcg aggccgaggc ggcggacgga cgcctccctg
     8041 gccctccgct tgcccgacag gagggggagc cgcggctcgc ccagggcctt gcagagggtc
     8101 agcacggcgg gcaggggggc ggagaccgtc tggatcccgt cctcgaggac ccgctcggcc
     8161 ctgagacgac ccgcgccggc gctcccggcc cggtccgccg cgctgccgcg gtccagggca
     8221 aaggagccga cgtagctcac cacgggaagg ccgaggaagg cggcgacctc cgggccgacc
     8281 tggcccgtgt cgccgtccgt cgccttttcc ccgcagaaga cgaggtcgaa ggggccgagg
     8341 aggccgaggg cggcggccag ggcccgcgag gtggcccagg tgtcggcccc gccgaactcc
     8401 ctgcccgtga cgaggacggc ctcgtcgcag cccatcgcca aggcctcgcg cagggcggac
     8461 tcggcctggg gcggccccat ggagagggcc aggatcgaga cgccagggtc ctcgtccttg
     8521 agccgcaggg cggcttccag ggcgtagagg tcgagcgggt tgacgatgga ggtatcctcg
     8581 gagcggatca cggtgcccgt ctccggatcg agccgcaagg agtcggtctc cggcacttgc
     8641 ttgacgagga cggctagttt catggcgagg gcacctccga ggcgggagcc gtgggcgccg
     8701 cggcggcaag ggccgcggcg gcgcgggcgg gccccgcgct tcggagccat tctgtcccag
     8761 gaacgcgaaa cgggcaagcg aaattgaaaa aatgtttctt tacaaccgcg tttccgggtg
     8821 ataaggtgta atggtgtttc agaccatgct cgccaggccc cgccatccgc ggggaaaggc
     8881 gggtcggagg ctggcttcat gatcgatgtg acgattaccc aggaaatgct cgacggcttc
     8941 acgacggaga gccggaacga gcgctcgatg gacatcgaca aggcgggcag ccgcgagatc
     9001 ctcgccatca tcaacgagga ggacaagacc gtccccttcg ccgtcgagcg cgagctcgac
     9061 catatcgccg ccctggtgga cgacgtcgtc gcggccttcc gcgggggcgg gaggctcttc
     9121 tacataggag ccggcacctc gggccgcctg ggcgtcctgg acgcctccga gtgcccgccc
     9181 acctacggcg tgcccccgac catggtccag ggcctcatcg cgggcggcct ccccgccctg
     9241 gtccgctcca tcgagggggc ggaggacgac ctggaggccg gggctaagga gctcgaggcc
     9301 gcgggcttcg gcccgggcga cgtcctcgtg ggcatcaccg cctcgggcca ggcgccctac
     9361 gtcctgggcg ccatggccaa ggcgaaggcc gctggcgccg tcgtcgcggc catcagctgc
     9421 aacaagggct ccaagacctt ccagtacgcg aagc
//
LOCUS       DAOOSJ010000085         9423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4690_length_9423_cov_5.986123, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000085 DAOOSJ010000000
VERSION     DAOOSJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4690_length_9423_cov_5.986123"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PLG14_05695"
     CDS             complement(<1..1472)
                     /locus_tag="PLG14_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48663.1"
                     /translation="MSFFSRRATFAALAGLCLAAGSLAALDRDSSLDSYYRYPVSIGG
                     QYQSLSPFAGFRSDFDLYDLAALVRYPLPGLPEVQPLFLGGVIQFAPRGDDQSWEHKD
                     LYALLGAAYSSRFSKTFELGADLSLGASLSLYPSLFPETGTVGNYNFLAQAGGRLALI
                     PSYNFAIEFAPSLKYLRSLGAVADFDGLILGIGLSAHVRLGEDPDAAKAGLRSLRLGD
                     AGSTSVYPAMQSWYAKNPFAKITVTNAESFAVQDVEISFFQKGYMDSPTLCARIPELK
                     AGESREVGLLASFNSEVFRNEGVTPLSGEIVATYSGRGRPGEQRASLSYDLQDKSAIV
                     WDDDRKAAAFVTPADGALRNYASYIRQINKEELVAGYSETVQFACQVFHALGELGILY
                     QVDPTQPFASVKGGAVTVDSVSLPRQTLKRITGDCDDLTVLYASLLESAGVETALVTV
                     PGHIYAAFSTKTAGKAFGEIHPDRGMTIAVNDELWIPVEIT"
     gene            complement(1581..2666)
                     /locus_tag="PLG14_05700"
     CDS             complement(1581..2666)
                     /locus_tag="PLG14_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX48664.1"
                     /translation="MFDALYEYLRGAIGFSLIVGGISLRLAHAKERGLKAFGGLCLAV
                     GALFSMSALDSFARLPVDLDNLVFQSLMLVLGLAVIDISLYVFGQERHSGGRKRLARA
                     GAAYAAALAVLPLLDYALGLGSSIANVEDGLVRGPVHAAATNAAYLWPIAATIASIRI
                     ARWKPADVASTHPESRRLIIAFRAILLVLAAILASLFLSLRIPYRLAHLVLELLLLAM
                     YFYVVKNPRALMRLRAEIEKEGGRRLSLGEEEAALIEKRLEGIAEKREIVLDEGFDLR
                     RLAELLGLPAYRLSAFFGSRLGTSFPAWRNRLRIDYVRERMAERPDLTILEISLEAGY
                     RSKASFNEQFGKIAGMSPSEYRKTLAR"
     gene            complement(2843..4642)
                     /gene="pepF"
                     /locus_tag="PLG14_05705"
     CDS             complement(2843..4642)
                     /gene="pepF"
                     /locus_tag="PLG14_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOX48665.1"
                     /translation="MSETKLLERSKVDKRYLWNAESVYPDRAAWRAELEAVKAGLPAL
                     AARASTIASGGAKGLADTLELMLGLLDRTGRLYVYASMSQAVDTADAEAQGMNGQAGG
                     LFGQFMAAASFLDPELLAVGKPALDGWIAAEPRLAVYGHYVENLFRKQAHVRSAEVEE
                     VLGMVFEVFQSAENTHDMLVDADMKFAPAKGGAEVAQGTVEKLLSHPDREVRRSAWRS
                     YCDSHVALENSLAASLATAMKRDVFNARARRFGSSIEAALFEHDIPRAVFDSTIETFK
                     KNLPVWHRYWRVRRKALGVEKLEHCDIWAPIAKVEPVVPYEKAVDWICGALEPLGKEY
                     VGTLRRGCLEDRWVDVYPTVGKSSGAFSTGWKGISPFVKMQYVDDLSSMSTLAHELGH
                     SMHSWHTWKRQPSVYADYSIFVAEVASNFNQAMVRASLFEKEKDPQFQIALIEEAMDN
                     LHRYFFIMPTLARFELEMHDRLEKGGTATAEDMNELMADLFAEGYGGEMEIDRRREGS
                     TWAQFGHLYMNYYVFQYATGISAAHALAAPILAGDGAAAARYVEFLSAGSSDYPVEVL
                     RRAGADMRTPAAMEKCFGVLEGLVDRLERLTAK"
     gene            4746..5267
                     /locus_tag="PLG14_05710"
     CDS             4746..5267
                     /locus_tag="PLG14_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX48666.1"
                     /translation="MDDERRRRLEGAYLAEEAGLRAWIGRRLGPESAEDLAQELFLRA
                     LANLDSLEPVRDLAAWLWRSARNAVVDAWRAHARRPASSAPEDFDGFVDGALGSVHDG
                     LEREELLGALAKAIAGLPPAQREVIEAQALDGESFASIAERTGTPPDTLAARKRYALA
                     RLRKALADYERED"
     gene            5269..>5498
                     /locus_tag="PLG14_05715"
     CDS             5269..>5498
                     /locus_tag="PLG14_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48667.1"
                     /translation="MEGHRRFWPKGRFSPLQIAGMVAGGFVLAAGFAFFFGWIVMLLW
                     NWLMPEIFRLPEIGYWQAWGLVLLSHILVKPG"
     assembly_gap    5499..5598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5600..5998)
                     /locus_tag="PLG14_05720"
     CDS             complement(5600..5998)
                     /locus_tag="PLG14_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX48668.1"
                     /translation="MKGNIVALLAIGALIVALSPAYAAPATSQPRLPARIDYKSLSSL
                     LEEPSSRLLLLDVRTAEEFGAGHIPGAVLAPYDSLETGFREPDKGRPVVVYCRSGRRS
                     AIALETLRRMGYTNLSDFGAVDNWKGRLAR"
     gene            complement(6162..6617)
                     /locus_tag="PLG14_05725"
     CDS             complement(6162..6617)
                     /locus_tag="PLG14_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48669.1"
                     /translation="MRLKYFFGVFLVAAISAAAVAMSGGKLAAFVDLPTYVVVLAIPA
                     AIAFASWPVRDIGRAFSAPFDPAAGRKELEKSLLFFSQLRRWVLVAALLGTMIGLVCI
                     LAYADGRNLDRLGRNLAVMFLCVTNALLFCLVVPLPLESLASRRLAELE"
     gene            complement(6629..6766)
                     /locus_tag="PLG14_05730"
     CDS             complement(6629..6766)
                     /locus_tag="PLG14_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48670.1"
                     /translation="MADTAIRGGRAAVLGAAVGERLGAAVQGFGELKGAASTAPSGRF
                     K"
     gene            complement(7002..7547)
                     /locus_tag="PLG14_05735"
     CDS             complement(7002..7547)
                     /locus_tag="PLG14_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX48671.1"
                     /translation="MESLDPGAAPGEPEAGVGYEALWREWRPRAAAYARAFGGLSAED
                     REELADEAVARAWEARARFRAGSPFAPWFFQIVRRLALDALRRRCEEPKPPAFFEGQG
                     SSPPEAEEAALRDAEAEFVRRFVAGLPSRDRELSSLVYGQGLGLAEAACVLGVPAGTA
                     KWRLSEIRKALRRAWEREYGE"
     gene            complement(7884..>9423)
                     /locus_tag="PLG14_05740"
     CDS             complement(7884..>9423)
                     /locus_tag="PLG14_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOX48672.1"
                     /translation="GGKCNLRFDDTNPEKEDMSYVEAIKRDVAWLGCDWEDREFYASD
                     YYEQLYGLAVKLIKKGKAYVDDLSAEEMSEYRGTNVPDKNNITITPPGRNSPWRDRSV
                     EENLDLFARMRAGEFADGAKTLRAKIDMAHPNLLMRDPPMYRIRREPHYRTGTAWCIY
                     PMYDFQHPLSDAIEGVTHSLCSLEYEIHRPLYDWFIREAEVFPSRQIEFARLNLSHTV
                     LSKRWLLRLVKEGYVSGWDDPRMPTLSGLRRRGYTPESIRDFISRIGVAKTDSLVDVQ
                     LLEHCLREDLNKRALRVMAVLKPLKLVVENWPAGKVEELDAVNNPEDPAAGSRKIPFS
                     GELYIERDDFQEVPPPKYFRLFPGNSVRLRYGYIVTCTGFDKDPATGEVTAVRCVYDP
                     ATHGGDAPDNRKVKGTIHWVSAARAVPLEARLFEHLFLAERPQDVPAGVDWVTTINPA
                     SLEVVKGAFGEPSLAAAKPGDRYQFERLGYFSCDPDSKPGAPVFNRSVSLRDSWAKIE
                     KKNA"
BASE COUNT         1486 a         2977 c         3458 g         1402 t
ORIGIN      
        1 gtgatctcca cggggatcca cagctcgtcg ttcacggcga tggtcatgcc gcggtcgggg
       61 tggatctcgc cgaaggcctt gccggcggtc ttcgtggaga aggcggcgta gatgtggccg
      121 ggcaccgtga ccagggccgt ctcgacgccg gccgactcga ggaggctcgc gtagagaacg
      181 gtgaggtcgt cgcagtcgcc cgtgatccgc ttcagcgtct gccggggaag gctcaccgag
      241 tccaccgtca cggcgccgcc cttgacggag gcgaagggct gggtcggatc cacctggtag
      301 aggatgccga gctcgcccag ggcgtggaag acctggcagg cgaactggac ggtctcggag
      361 tagcccgcga cgagctcctc cttgttgatc tgccttatgt agctcgcgta gttgcgcagg
      421 gcgccgtcgg ccggagtgac gaaggccgcc gccttgcggt cgtcgtccca gacgatcgcg
      481 ctcttgtcct ggaggtcgta ggagagcgag gcccgctgct cgcccggcct gccccttccc
      541 gagtaggtcg cgacgatctc cccggagaga ggcgtcacgc cctcgttccg gaagacctcc
      601 gagttgaagg aggcgaggag gcccacctcg cggctctccc cggccttgag ctcgggaatc
      661 cgcgcgcaga gggtcggcga gtccatgtag cccttctgga agaaggagat ctcgacatcc
      721 tgcacggcga aggactcggc gttcgtcacg gtgatcttcg cgaagggatt cttcgcgtac
      781 cagctctgca tggcgggata gacggaagtc gaccccgcgt cgccgagcct gaggctccgg
      841 aggcccgcct tggccgcgtc cggatcctcg cccaggcgca cgtgggcgga caggccgatg
      901 cccaggatga ggccgtcgaa gtcggctacc gcgcccaggg agcggaggta cttgagggag
      961 ggagcgaact cgatcgcgaa attgtaggag ggtatgaggg ccaggcgccc gccggcctgg
     1021 gccaggaagt tgtagttgcc caccgtcccc gtctcgggaa agaggctcgg atagagggag
     1081 agggaggcgc ccagggagag gtcggctccg agctcgaaag tcttcgagaa cctggaggag
     1141 tatgcggctc cgaggagggc gtagaggtcc ttgtgctccc aggactggtc gtcgcctcgg
     1201 ggcgcgaact ggatgacgcc gcccaggaac aggggctgga cctcgggcag gcccggcagg
     1261 gggtagcgca cgagggccgc gaggtcgtag aggtcgaagt cggagcggaa gcctgcgaag
     1321 ggcgagaggg actggtattg gccgccgatc gagacggggt agcggtagta ggaatccagg
     1381 ctcgaatctc tatcaagggc ggcaagggag ccggccgcga ggcaaagacc cgctagcgct
     1441 gcgaaagtcg cgcgacgaga gaaaaaggac attatggata cccccgacta tcatcgactc
     1501 gccgagggat tccgcagccg agattgtcga gggagaggct acagcgctcg gcggccgccg
     1561 tcgtccggat tcgcgaatcc ttatcgggcc agggtcttcc tgtactcgct agggctcatc
     1621 ccggcgatct tcccgaactg ctcgttgaag gaggccttgg agcggtagcc ggcctccagg
     1681 gagatctcga ggatcgtgag gtcaggtcgc tcggccatcc gctccctgac atagtcgatg
     1741 cgaaggcggt tcctccaggc ggggaacgag gttccgagcc tcgatccgaa gaaggcggag
     1801 agacgatagg cgggcagccc caggagctcg gcgagcctcc gcaggtcgaa gccctcgtcc
     1861 aggacgatct ccctcttctc cgcgatcccc tcgagccgct tttctatgag agcggcctcc
     1921 tcctccccga ggctcaggcg gcggccgccc tccttctcga tctccgcgcg gaggcgcatg
     1981 agggcgcggg gattcttgac gacgtagaag tacatcgcga ggaggaggag ctccaggacg
     2041 agatgcgcca gtctgtaggg aatccggagc gagagaaaga gcgaagcgag gatggcggcg
     2101 aggacgagga gtatggcacg gaaggcgatg atgaggcggc gcgactcggg gtgcgtggag
     2161 gcgacgtccg cgggcttcca gcgggctatg cgtatggagg cgatggtcgc cgcgatcggc
     2221 cacaggtagg cggcgttcgt cgcggcggcg tggacgggtc cgcgtacgag tccgtcctcg
     2281 acgttggcga tcgaggatcc gaggcccagg gcatagtcga ggaggggcag gacggcgagg
     2341 gccgcggcgt aggccgctcc cgcgcgggcg agccgcttcc tgccgcccga atgccgctcc
     2401 tgcccgaaga cgtagagcga gatgtcgatg accgcgaggc ccaggacgag catgagggac
     2461 tggaagacca ggttgtcgag gtcgacgggg aggcgggcga aggaatccag ggcgctcatg
     2521 gagaagaggg cgccgacggc caggcagagt ccgccgaagg ccttgaggcc ccgctccttt
     2581 gcgtgggcga gcctcaagga gatgcccccg acgatgaggg agaagcctat ggctccgcgc
     2641 aggtattcgt agagggcgtc gaacatggaa gcctcgcaag ccgggaagag tcgcgggatt
     2701 atagcacccc gcttgtcccg aggcgaggac aaagattatc tttccgcgcg cctaagcggg
     2761 gccgagggga ggcgcggcgc ccaaaaggcc gggcctcacg gcccggcccc gcgccggaaa
     2821 cagagagggc gtgccgggcg gcctacttcg cggtaagcct ctcgagcctg tccacgaggc
     2881 cctcgaggac gccgaagcat ttctccatcg cggcgggcgt gcgcatgtcg gccccagccc
     2941 gcctcagcac ctcgaccgga tagtccgagg agccggcgga gaggaactcg acgtagcgcg
     3001 cggccgcggc tccgtcgccc gcgaggatcg gggccgcgag ggcgtgggcc gcgctgatgc
     3061 cggtcgcgta ctggaagacg tagtagttca tgtagaggtg gccgaactgg gcccaggtcg
     3121 agccctcgcg gcgcctgtcg atctccatct cgccgccgta gccctcggcg aagaggtcgg
     3181 ccatgagctc gttcatgtct tcggccgtcg ccgtgccgcc cttctccagg cggtcgtgca
     3241 tctcgagctc gaagcgcgcc agggtcggca tgatgaagaa gtagcggtgg aggttgtcca
     3301 tcgcctcctc gatgagggcg atctggaact gcgggtcctt ctccttctcg aagaggctcg
     3361 cgcggaccat cgcctggttg aagttggagg cgacctccgc gacgaagatc gagtagtcgg
     3421 cgtagacgga gggctggcgc ttccaggtgt gccagctgtg catcgagtgg ccgagctcgt
     3481 gggccagggt gctcatcgag gagaggtcgt cgacgtactg catcttcacg aagggcgaga
     3541 tgcccttcca gccggtggag aaggcgccgg agctcttgcc gacggtcggg tagacgtcca
     3601 cccagcggtc ctccaggcag ccgcggcgca gggtccccac gtactccttg ccgaggggct
     3661 cgagggcgcc gcagatccag tcgacggcct tctcgtaggg gacgacgggc tcgaccttgg
     3721 cgatgggcgc ccagatgtcg cagtgctcga gcttctcgac ccccagggcc tttcgcctga
     3781 cgcgccagta gcggtgccag acgggcaggt tcttcttgaa ggtctcgatg gtcgagtcga
     3841 agaccgcgcg ggggatgtcg tgctcgaaga gggcggcctc tatggaggag ccgaagcggc
     3901 gggcgcgggc gttgaagacg tcgcgcttca tcgccgtggc cagggaggcg gcgaggctgt
     3961 tctcaagggc gacatgggag tcgcagtagc tccgccaggc cgagcgcctg acctcgcggt
     4021 cggggtgcga gaggagcttc tcgaccgtgc cctgggcgac ctccgctccg cccttggcgg
     4081 gggcgaactt catgtccgcg tcgacgagca tgtcgtgggt gttctccgcg ctctggaaga
     4141 cctcgaagac catgcccagc acttcctcga cctccgcgga gcggacgtgc gcctgcttcc
     4201 ggaagaggtt ctccacgtag tgcccgtaga ccgcgaggcg gggctccgcg gcgatccagc
     4261 cgtcgagggc cggcttgccg accgcgagga gctcggggtc gaggaaggag gccgcggcca
     4321 tgaactggcc gaagaggccg ccggcctggc cgttcatgcc ctgggcctcg gcgtccgcag
     4381 tgtcgacggc ctgggacatg gaggcgtaga cgtagaggcg gcccgtccgg tcaaggaggc
     4441 cgagcatgag ctcgagggtg tcggcgaggc ccttggcccc gccggaggcg atcgtgctgg
     4501 cgcgggccgc cagggcggga aggcccgcct tgaccgcctc gagctcggct cgccaggcgg
     4561 cgcggtcggg atagacgctc tcggcgttcc agaggtagcg cttgtcgact ttggatcgtt
     4621 ccaggagttt ggtttcgctc atggatgaca ttgtaaaagc ctttcccctc cgggtcgagg
     4681 cacaaaacga tgataatccg cccaaggact agacaaattc ccggactcgc gcgtctttag
     4741 tatcgatgga cgacgaaagg cggaggcgcc tcgaaggcgc gtatctcgcg gaagaagcgg
     4801 gcctgcgcgc ctggataggc cggcggctcg gccccgagag cgcggaggac ctggcccagg
     4861 agctcttcct cagggccctc gcgaacctcg actccctgga gccggtgcgc gacctcgcgg
     4921 cctggctctg gcgctcggcg cgcaacgcgg tcgtcgacgc ctggcgggcg catgcgcgcc
     4981 ggccggcctc gtcggccccc gaagacttcg acggcttcgt cgacggtgcc ctcgggagcg
     5041 tccacgacgg gctcgagcgg gaggagctcc tcggggccct cgcaaaggcc atcgccggcc
     5101 tgccgcccgc ccagcgggag gtcatcgagg cgcaggccct ggacggggag agcttcgcct
     5161 ccatcgcgga acggacggga acgccccccg acaccctggc tgcgcggaag cgctacgcgc
     5221 tcgccaggct caggaaggcg ctggccgact atgaaaggga ggattgagat ggaaggacac
     5281 agacgattct ggcccaaggg ccgcttctct ccgcttcaga tcgcgggtat ggtcgcgggc
     5341 ggcttcgtcc tcgcggcggg cttcgccttc ttcttcggct ggatcgtgat gctgctctgg
     5401 aactggctca tgccggagat cttccgcctg cccgagatcg gctactggca ggcctggggc
     5461 ctcgtcctcc tctcccacat cctcgtcaag cccggctann nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnncc tagcgcgcga gcctcccctt ccagttgtcc accgccccga
     5641 aatcggagag gttcgtgtag cccatcctcc gcaaggtctc cagggctatg gccgaacgac
     5701 ggccgctcct gcagtagacg acgacgggcc tgcccttgtc gggctccctg aagcccgtct
     5761 ccaggctgtc gtagggagcg aggacggctc cgggtatgtg gcccgcgccg aactcctcgg
     5821 ccgtgcgcac gtccaggagg agaaggcggg aggaaggctc ctcgaggaga cttgagaggc
     5881 tcttgtagtc gattcgcgcg ggcagccgtg gctgggacgt ggccggagcg gcgtaggctg
     5941 gggacagagc tacgatcaag gcgccgattg ccaacaaagc tactatattg cctttcatgc
     6001 atatactata ctgcggttta tccccgcggg caacgaggag tccgcacggc gagaaggggc
     6061 ggcgccgcct ccgcctgccc cgcctccgcc tgccccgccc cgaggcgtcc cggagcgcgg
     6121 ctggccgtcc cgagtcctcc gaaagccttg cccggtccgg cctactccag ttccgcgaga
     6181 cggcggcttg cgagcgactc gaggggcagg ggcacgacga ggcagaagag gagcgcgttc
     6241 gtgacgcaga ggaacatgac cgcgagattc cgcccgagcc ggtccaggtt cctgccgtcc
     6301 gcgtaggcga ggatgcagac gaggccgatc atcgtgccga ggagggcggc gacgaggacc
     6361 cagcgccgga gctgggagaa gaacaggagg gacttctcga gctccttccg gcccgccgcc
     6421 gggtcgaagg gggcggagaa ggcccttccg atgtcccgca ccggccagga ggcgaaggct
     6481 atggccgcgg ggatggccaa gaccacgaca taggtcggca ggtcgacgaa ggccgcgagc
     6541 ttcccgcccg acatggctac ggccgccgcc gagatcgcgg cgacgaggaa cactccaaag
     6601 aaatacttga gtctcatggc gtctcctttc acttgaaccg gcccgaggga gccgtggagg
     6661 ccgctccctt gagctcgccg aagccctgga cggcggcgcc gaggcgctcg ccgaccgcgg
     6721 cgccgaggac ggcggcccgg ccgcccctga tcgcggtgtc ggccatcgcc gccccgagcg
     6781 ggctgccgtc cgaaagcgcc gacaggcctg ccgaggcgag gaggatcgcg gcggcggcgg
     6841 cctcgggcag ggcgcgggga cgccggcgcc gcggcgcgga cgccgctccg ggcccgacgc
     6901 cgcgatccgg cgcccgcggc gcggctggac gaaagcgccg ctcctcctcc agctccgccg
     6961 cgaaatgcgt ccgcaaggcc gcctcagggt cgaaccgggc cttactcccc atattcccgc
     7021 tcccaggccc gcctgagggc cttcctgatc tctgaaagcc gccacttcgc ggtgcccgcc
     7081 ggaacgccga ggacgcaggc cgcctcggcc aggccgagcc cctggccgta gacgaggctc
     7141 gaaagctcgc ggtcccgact tggcagaccg gcgacgaagc gccgcacgaa ctcggcctcc
     7201 gcgtcccgga gcgccgcctc ctcggcctcg ggaggcgaag agccctgtcc ctcgaagaag
     7261 gccggcggct tgggctcctc gcaacggcgt ctgagcgcgt cgagggcgag cctccgcacg
     7321 atctggaaga accagggcgc gaaggggctg cctgcgcgga agcgcgcgcg cgcctcccaa
     7381 gcccgggcga cggcctcgtc ggcgagctct tcgcggtctt cggccgagag gccgccgaag
     7441 gcgcgcgcgt aggccgcggc cctggggcgc cactcgcgcc agagggcctc gtagccgacg
     7501 cccgcctcgg gctcgccggg cgccgctccc ggatccaggc tctccattcg ccctactcta
     7561 cgcgctggac ggcggacggt tgggaaaatc ggcggaattc acgcgcgagg gaccgcagcg
     7621 gaaatcaccg cccccgccaa aggagcgtcc cgcgccgggc tccgggacgg agaccggcgc
     7681 ccaggccgac cccgaaggga cgggggcggg atttgagcgg agggcccggt ccgcgcgcgg
     7741 cccttgctga aggcgccggg cgcgcggggt tcggcgaccg cgtcccgcga ccgcgcgcgc
     7801 ggacgcgccc agggcttccc caaaaagaag cgggggcccc gaagggcccc cgcgagtctc
     7861 aggaagcgcc tgcgcctttc ggctcacgcg ttcttcttct cgatcttcgc ccagctgtcg
     7921 cggagcgaga ccgagcggtt gaagacgggc gcgcccggct tcgagtcggg gtcgcaggag
     7981 aagtagccga ggcgctcgaa ctggtagcgg tcgcctggct tggccgcggc gagggaaggc
     8041 tcgccgaagg cgcccttcac gacctccagg gaggcggggt tgatcgtcgt cacccagtcg
     8101 acgcccgcgg gcacgtcctg cgggcgctcc gcgaggaaga ggtgctcgaa gagccgggcc
     8161 tcgaggggga ctgcccgggc cgcggagacc cagtggatgg tgcccttcac cttgcggttg
     8221 tcgggggcgt cgccgccgtg ggtcgcggga tcgtagacgc agcgcaccgc cgtgacctcg
     8281 ccggtggcgg gatccttgtc gaagcccgtg caggtgacga tgtagccgta gcggaggcgc
     8341 accgagttcc ccgggaagag gcggaagtac ttgggaggcg ggacctcctg gaagtcgtcg
     8401 cgctcgatgt agagctcgcc cgagaagggg atcttgcggg agccggccgc cggatcctcg
     8461 gggttgttca ccgcgtcgag ctcctcgacc ttgcccgcgg gccagttctc cacgacgagc
     8521 ttgagcggct tcaagaccgc catcacgcgg agggcgcgct tgttgaggtc ctcgcgaagg
     8581 cagtgctcga ggagctggac gtcgacgagg gagtcggtct tggcgacgcc gatgcgcgag
     8641 atgaagtcgc ggatcgactc cggcgtgtag ccgcggcggc ggaggcccga gagggtcggc
     8701 atgcgcgggt cgtcccagcc ggagacgtag ccttccttca cgaggcggag gagccagcgc
     8761 ttggagagga ccgtgtgcga gaggttgagc cgcgcgaact cgatctggcg ggaggggaag
     8821 acctccgcct cgcggatgaa ccagtcgtag agggggcggt ggatctcgta ctcgagggag
     8881 cagagcgagt gggtcacgcc ctcgatcgcg tcggagaggg ggtgctggaa gtcgtacatc
     8941 gggtagatgc accaggcggt cccggtccgg tagtggggct cgcggcggat gcggtacatg
     9001 ggcgggtcgc gcatgaggag gttggggtgc gccatgtcga tcttggcgcg cagggtcttg
     9061 gcgccgtcgg cgaactcgcc cgcgcgcatg cgcgcgaaga ggtcgaggtt ctcctccacg
     9121 gagcggtcgc gccaggggct gttgcgcccg ggcggggtga tcgtgatgtt gttcttgtcg
     9181 gggacgttgg tgccgcggta ctcgctcatc tcctccgcgg agaggtcgtc gacgtaggcc
     9241 ttgcccttct tgatgagctt gacggcgagc ccgtagagct gctcgtagta gtcggaggcg
     9301 tagaactcgc ggtcctccca gtcgcagccg agccaggcga cgtcgcgctt gatcgcctcg
     9361 acgtaggaca tgtcctcctt ctcggggttc gtgtcgtcga agcggaggtt gcacttgccg
     9421 ccg
//
LOCUS       DAOOSJ010000086         9394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4704_length_9394_cov_6.661955, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000086 DAOOSJ010000000
VERSION     DAOOSJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_9394_cov_6.661955"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLG14_05745"
     CDS             <1..617
                     /locus_tag="PLG14_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOX48673.1"
                     /translation="SSRERTSAVDFRRIDPRSLSMNPFSLIAEEWMLVTAGAPGDWNT
                     MTASWGGFGHLWNRDVAFVFVRPTRHTYGFMERESRFSLSFFGPGMRQALALCGSLSG
                     RDVDKAQAAGISPRVFDAAASPGEPAGRGGSPAPAGHGGPGGADSANVIAFEEARLVL
                     ALRKLHAQDLDPACFVDPSINDHYAALDWHRLYVGAVEAAWSRD"
     gene            632..>1234
                     /locus_tag="PLG14_05750"
     CDS             632..>1234
                     /locus_tag="PLG14_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48674.1"
                     /translation="MERTVLGSLRDRGLSFVLPSEIAAEAWLERALVLGGEEALEAGR
                     FLGWDSFKELVSAEAEGAGPADDFLRTIFAASYLSANAKEPRLRVLVPPEHAGLHAPF
                     APCVAAGLPRLRSLSLAVRRRGGPPPSGALADWLDIHRAYEAFLSERSLYEPSYLPRR
                     LRDPGRRFLVLFPELIEDFAEYEEGLKASGFVRVLSLAEAL"
     assembly_gap    1235..1334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1335..3535
                     /locus_tag="PLG14_05755"
     CDS             <1335..3535
                     /locus_tag="PLG14_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOX48675.1"
                     /translation="AAAPLRLARPGSGLAELRSLLRGIGRLLDEGEAPESLVVTVCGL
                     DAYRPYLEREARLLSVPLDFRSGKALSAYPGPRLFKAAGEAAGSGFAFDAMRDLLLSP
                     AWPWKDPALNRALVRAGAARCALAPWPAAPGEGGGAARGAAPAPEQTPAAGRTAGRLV
                     DPWEASLPGAEGGAALLERYRALRRRIAAMSAAESFAALRKAYAAFRKELFSDEGWEP
                     LADASLARAVEELEGLCRAEEKAGLPVESPFGIFLRVLDQKRYVPQGGSGGVRVYEWR
                     VGAGLAPERHFVLGASQDALSVTYSRFGFLREDERRGLGAEEADATPAFIAAYAESGS
                     QVLFSCPREGFSGERPPHGILLSRAEPGAFPPGSDEYEEELLWLSGEGQAPGRLHAIQ
                     RRGLLRGLGAASAGGRKGAVLPPALAATAAARLRGEDGTLRLSSTAIDEYGRCPFAYL
                     YERLLRATEEKAGLEFADAPFLGEVYHEALARLFARIAKEDGRFRPERRLEYADLAGE
                     ALDAAFAAFARRRGPFAGVVLSAYRGRMELYLRRLMEREAELFPGLEVGPLEEDFLLR
                     YPEGGDPERGVPPRVALSGRIDRLSLREGRAVVVDYKKGNYPTRAEVAPSVDGSLAEA
                     QVPAYLRLVGASEAGSGGVESAWYLSVEGSSQLEPGSAACAFGPAAFKPYVPEESLPS
                     FLAAFDAALGRTASGILAGDYPFAPKGAQEVACADCASRGICRERYALRL"
     gene            3550..7536
                     /locus_tag="PLG14_05760"
     CDS             3550..7536
                     /locus_tag="PLG14_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX48676.1"
                     /translation="MAEAVIRYKRARGLEGPQAEAALAEENAVVAAGAGSGKTTVLAA
                     RYVRLLEEGRLRDGSRVHARNVLVLTFTRKAAAEMHGRIYGALAEAAALAERAAAAER
                     AAGGAAGGAAGAGAAGAGAAEAGDAAGAAPSGETAAGPGGEAAGLAVHMAACLAEFSQ
                     AQISTFDSFAGRIARSGSSRFGLAPDFQVDDGAARESALRLALDFILERREEAAMREL
                     VAANGFTETQEALAGLAVERMPLSSPPDFAAMHARQEGLLRDGLAKLRAELLDERAAA
                     LEYAGGKAPAGVKAWLDAFAADPFAAAPAGACAAGPAAGLADRAAGPTGAEGADPFLA
                     WLKGFEGLRKPGSNSTSEAGLYLNGAVDGFKTLASAYRAHAAALAAHPGRLALYRLLD
                     EFRPRWDQARRAAKTLSFRDAATMAFDLLSRDPGLRAVYKGLYRYVMIDEFQDDDELQ
                     KRVLYLVAEREGRRETGEPTPGELEPDKLFFVGDEKQSIYLFRGADVSVFRALAGELA
                     ASAPGGKPRRLVLSRNFRSEPGLIELYNRLFPVVMRPEDGRESEPHEAAFEPLLGREP
                     TPGAAPEIRYLELPYRPDSAEGLRSRAECEAWAIAEFIRDSVRTGSLAVADRESGRAR
                     PARYEDFAVLFRATGKQIHLEKYLRLLGVPYASESACGLFSEAVAGDLYYALRLAAHP
                     EDRQALAAYLRSPFASVDDEKAAGLLLAGEGGLESPFAAADPAFGRARGTMAALRGMA
                     DRETIASCLSYLWYEAGYRAALLSSPGGRAFEEHFELVHSLAVAADAEGRPLAAFVAD
                     LEPLVGSPERLDDAEIPREAARGVRLMTVHKSKGLEFPVVVVPQANNAGRNGGAPSWA
                     WDDEAGATFRLPAPGGDPKRRDNIFYERAAEERAARQRAEAKRLLYVALTRAESQVLV
                     TATAPRNEEKEKPKSFRSFLLPAMGLRAGSGPGAAAPGAAAPGAAAPGAAGNGSPGEV
                     AEAGGREDEAARAADAVLGEPVYVSELETGRLRRLPSGVLAGLIRERSDEDYEALLGS
                     ARARAAKPAMPDAAPVGRRAAPRLRAVSAIAAGWSASRAVGAEGKGDAAAQRAEPLAV
                     APGLERPEGLPPELWGSLVHALIERALIERALAGTAAPREEELEPSLRSSLQSALPSE
                     SSLKAAMERAARLAEVFLGSALGRRALAAKERLVEWPLAIRYEVPQGAGAAAAARRVA
                     RGQADLVAVEEGRAFVVDYKTDGEIEAGRYDLQVAAYRRAAEAVLGLPTEAYIFYLHG
                     GGRAVPVAESAPGLADAPLPEPEPFEFSFAPATGEAPAHGQAPAHGQAPATGQETARP
                     PAPREAGG"
     gene            7533..9227
                     /locus_tag="PLG14_05765"
     CDS             7533..9227
                     /locus_tag="PLG14_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOX48677.1"
                     /translation="MSGRKSGGGSSPAPREGSSQAPAEALSSAELQDAYEDQRLLVEI
                     GRELIVEKDQGRLLGRILDACRGITGADAGSIFLCEQGDRGPLLRFKHAKTFSRPLAY
                     EEFTMPRDERSIAGYVSLSGKPLNVPDVYGLDPALPYRFNSGYDRSSGYRTKSMLVVP
                     MRGLKGEVIGVIQLINSKESPEAESEEAVSGREIADSVLLAGPEDFERLVFPFKERYE
                     GLMEAVANQAAIALENARMVRRLAEQFESFVRASVAAVESLDPATSGHSERVARSAVA
                     LMAAVDAEKDGRYGACSFGAEERLELEYAGLLHDFGKVYIDPRIFTKAKKLFERDYDC
                     LVWRLKYSGARAELACARREVEALRGGRGAEAEGERREAERAKAAVLEALGLVRALNE
                     PEATEGEPEERLALLLVAAPPAGLDLDGSPLPLLTEAERRDLSIRRGTLNEEERRLIQ
                     DHVRYTQAFVSRITWPPELAGVPEYCAKHHEMLDGSGYPAALKADRIPLQARMLAIAD
                     VYDALAARDRPYKKALPFETVRSIMEEEAAKGRLDEELLRLFFERECWNESASGPA"
BASE COUNT         1186 a         3623 c         3279 g         1206 t
ORIGIN      
        1 gaagctcgcg ggaaaggact agcgccgtgg acttccggag gatcgacccg cggtcgctgt
       61 cgatgaaccc cttctccctc atagcggagg agtggatgct cgtcacggcg ggcgcgccgg
      121 gggactggaa cacgatgacc gcgtcctggg gaggcttcgg ccacctctgg aaccgcgacg
      181 tcgccttcgt cttcgtgcgg ccgacgcggc acacctacgg cttcatggag cgggagtccc
      241 gcttcagcct ctccttcttc gggcccggga tgcggcaggc cctggccctc tgcggctccc
      301 tctccggccg cgacgtcgac aaggcccagg ccgcgggcat cagcccccgg gtcttcgacg
      361 cggcggcgtc gcccggggag ccggccgggc ggggcggttc cccggcccct gccggacatg
      421 gcggcccggg cggcgccgat tcggccaacg tcatcgcctt cgaggaggcc cgcctcgtcc
      481 tcgcccttag gaagctccac gcccaggacc tggacccggc ctgcttcgtc gatccttcca
      541 tcaacgacca ctacgccgcc ctcgactggc accgcctcta cgtgggcgcc gtcgaggcgg
      601 cctggagcag ggactagcct tgttcggagc catggagagg accgtcctcg gctccctccg
      661 ggaccggggg ctctccttcg tcctcccgag cgagatcgcg gcggaggcct ggctggagcg
      721 ggccctcgtc ctgggcgggg aggaggccct ggaggccggt cgcttcctgg gctgggacag
      781 cttcaaggag ctcgtctccg cggaggcaga gggcgcgggg ccggccgacg acttcctcag
      841 gaccatcttc gccgcgtcct atctatcagc caacgcgaag gagcccaggc tccgcgtcct
      901 cgtcccgccg gagcacgcgg gcctgcacgc gcccttcgcg ccctgcgtcg ccgcgggcct
      961 cccgcggctc cgctccctgt cgctggccgt ccggcgccgc ggcggcccgc cgccctcggg
     1021 agccctcgcc gactggctcg atatccaccg cgcctacgag gccttcctct ccgagcggag
     1081 cctctacgag ccctcctacc tgccgcgtcg cctccgggac cctggccgtc gcttcctcgt
     1141 cctctttccc gagctcatcg aggacttcgc cgaatacgag gaggggctca aggcctccgg
     1201 cttcgtccgc gtcctgagcc tcgccgaggc cctcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnngggcgg cggcgccctt gcggctcgcc cggcccggct ccggcctcgc
     1381 ggagctcagg agcctccttc gcggcatcgg ccgcctcctc gacgagggcg aggcgcccga
     1441 gagcctcgtc gtcaccgtct gcggcctcga cgcctaccgg ccctacctcg agcgcgaggc
     1501 ccgcctcctc tccgtgcccc tggacttccg ctccggcaag gccctctcgg cctaccccgg
     1561 cccccgcctc ttcaaggcgg ccggcgaggc ggccggctcg ggcttcgcct tcgacgcgat
     1621 gcgcgacctc ctcctttccc ccgcctggcc ctggaaggat ccggcgctca accgggccct
     1681 cgtccgcgcc ggggccgcgc gatgcgccct ggccccctgg ccggccgcgc cgggagaagg
     1741 cggcggcgcg gcccggggag cggcgccggc cccggaacaa acgccggccg ccggcaggac
     1801 ggcgggccgc ctcgtcgacc catgggaggc gagcctcccg ggggccgagg gcggagccgc
     1861 cctcctcgag cgctaccgag ccctgaggcg ccggatcgcc gcgatgagtg cggcggagag
     1921 cttcgccgcc ctgaggaagg cctacgccgc cttccggaag gagctcttct ccgacgaggg
     1981 ctgggagccc ctggcggacg cgagcctggc gcgcgccgtc gaggagctcg agggactctg
     2041 ccgcgccgag gagaaggccg gcctccccgt cgagtcgccc ttcggcatct ttctccgcgt
     2101 cctcgaccag aagcgctacg tcccccaggg cggctcgggc ggcgtcaggg tctacgagtg
     2161 gcgcgtcggc gcgggcctcg ctcccgagag gcacttcgtc ctcggggcct cgcaggacgc
     2221 gctctcggtc acgtactccc gcttcggctt cctccgcgag gacgagaggc gcggcctcgg
     2281 cgccgaggag gccgacgcga cgcccgcctt catcgccgcc tacgccgagt cgggctcgca
     2341 ggtgctcttc agctgtcccc gcgagggctt ctcgggggag cgccctcccc acggcatcct
     2401 cctctcccgg gccgagcccg gcgccttccc gccgggctcc gacgaatacg aggaggagct
     2461 gctctggctc tcgggcgagg gccaggctcc cggccgactc cacgccatcc agcggagggg
     2521 cctcctgcgg ggtctcggcg cggcgtcggc gggagggagg aagggagccg tcctgccccc
     2581 agccctggcg gccaccgcgg ccgccaggct ccgcggcgag gacggaaccc tccgcctcag
     2641 ctccaccgcc atcgacgagt acgggcgctg ccccttcgcc tacctctacg agcgcctcct
     2701 tcgcgccacg gaggagaagg ccggcctcga gttcgccgac gcgcccttcc tgggcgaggt
     2761 ctaccacgag gccctggcgc ggctcttcgc gaggatagca aaggaggacg ggcgcttccg
     2821 tcccgagcgt cggctcgagt acgcggactt ggcgggcgag gcgctggacg ccgccttcgc
     2881 ggccttcgcc cggagacgcg gccccttcgc gggcgtcgtc ctctcggcct acagggggag
     2941 gatggagctc tacctccgac gcctcatgga acgggaggcg gagctcttcc ccggcctcga
     3001 ggtgggcccc ctggaggagg acttcctcct ccgctatccc gaaggcggcg atcccgaacg
     3061 cggggttccg ccgcgcgtgg ccctctcggg ccggatcgac cgcctctccc tccgagaggg
     3121 cagggccgtc gtcgtcgact acaaaaaggg caactatcct acgcgggccg aggtcgcgcc
     3181 ctcggtggac ggaagcctcg ccgaggcgca ggtccccgcc tacctccgcc tggtcggcgc
     3241 gagcgaggcg ggctcaggcg gagtcgagtc ggcctggtac ctgagcgtcg agggaagctc
     3301 gcagctcgag cccggctccg cagcctgcgc cttcgggccc gccgccttca agccctacgt
     3361 accggaggag tccctgccct ccttcctggc cgccttcgac gcggccctcg ggaggacggc
     3421 ctccggcatc ctcgcgggcg actacccctt cgcgcccaag ggcgcgcagg aagtcgcctg
     3481 cgccgactgc gcctcgcggg gcatctgccg cgagcgctac gccctgcgcc tgtaagcgag
     3541 gttcccgcca tggccgaagc agtgatccgg tacaagcgcg cccgcggcct cgagggcccc
     3601 caggccgagg cggccctcgc cgaggagaac gccgtcgtag ccgccggcgc cggctccggc
     3661 aagacgaccg tcctcgcggc ccgctacgtc cgcctcctcg aggaagggcg gctccgcgac
     3721 ggctcgcggg tccacgcgcg caacgtcctc gtcctcacct tcacgcgcaa ggcggccgcg
     3781 gagatgcacg gccgcatcta cggcgccctc gccgaggcgg cggccctggc cgaacgcgcg
     3841 gccgccgccg aacgcgccgc tgggggcgcc gctgggggcg ccgcgggggc cggcgccgcg
     3901 ggggccggcg ccgcagaggc cggcgacgcc gcgggggccg cgccgagcgg cgagacggca
     3961 gccggaccgg gcggcgaggc ggccgggctc gcggtccaca tggcggcctg cctcgcggaa
     4021 ttctcccagg cccagatctc caccttcgac tccttcgcgg ggcggatagc ccgctcgggc
     4081 tcctcccgct tcggcctcgc cccggacttc caggtggacg acggcgcggc ccgcgagagc
     4141 gccctccgcc tggccctcga cttcatcctg gagaggcgcg aggaggccgc gatgcgcgaa
     4201 ctcgtcgcgg ccaacggctt cacggagact caggaggccc tcgccggcct ggccgtggag
     4261 cggatgcccc tgtcctcgcc gcccgacttc gccgcgatgc acgccaggca ggagggcctg
     4321 ctccgggacg ggctcgcgaa gctccgcgcg gagctcctgg acgagcgggc cgccgccctc
     4381 gagtacgcgg gaggcaaggc tcccgcgggc gtgaaggcct ggctcgacgc cttcgcggcc
     4441 gaccccttcg ccgccgcgcc cgccggcgca tgcgcggcgg gcccggccgc gggcctggcg
     4501 gaccgggccg cggggcccac gggcgccgaa ggggccgatc ctttcctcgc ctggctcaag
     4561 ggcttcgagg gcctgcgcaa gccgggctcc aactcgacga gcgaggcggg actctacctg
     4621 aacggggcgg tcgacggctt caagacgctc gcttccgcct atcgcgccca cgcggcggcc
     4681 ctggcggccc accccgggcg cctggcgctc taccgactcc tcgacgagtt ccgcccgcgc
     4741 tgggaccagg cccgccgggc ggcgaagacc ctctccttcc gcgacgccgc gacgatggcc
     4801 ttcgacctcc tctcgcggga tcccgggctc cgcgccgtct acaagggcct ctaccgctac
     4861 gtgatgatcg acgagttcca ggacgacgac gagctccaga agcgcgtcct ctacctcgtc
     4921 gcggagcggg aggggcggag ggagaccggg gagcccaccc cgggcgagct cgagccggac
     4981 aagctcttct tcgtcggcga cgagaagcag tcgatctacc tcttccgcgg cgccgacgtc
     5041 tcggtcttcc gcgccctcgc gggggagctc gccgcctccg cccccggcgg aaagcccagg
     5101 cgcctcgtcc tctcgcggaa cttcaggagc gagcccggcc tcatcgagct ctacaaccgc
     5161 ctcttccccg tcgtgatgcg gccggaggac ggccgcgaga gcgagccgca cgaggcggcc
     5221 ttcgagcccc tcctcggcag ggagccgacg ccgggcgcgg cgcccgagat ccgctacctc
     5281 gagctgccct acaggcccga ctccgcggag ggcctgagga gcagggccga gtgcgaggcc
     5341 tgggcgatcg cggagttcat ccgggactcg gtgaggaccg gctccctcgc cgtcgcggac
     5401 cgggagtcgg gccgggcgag gccggcgcgc tacgaggact tcgcggtcct cttccgcgcg
     5461 acggggaagc agatccacct cgagaagtac ctccgcctcc tgggcgtccc ctacgcctcc
     5521 gagagcgcat gcggcctctt ctccgaggcg gtggccggcg acctctacta cgcgctcagg
     5581 ctcgcggccc atcccgagga caggcaggcc ctggcggcct acctccgctc gcccttcgcc
     5641 tccgtggacg acgagaaggc ggccggcctc ctcctcgcgg gcgagggcgg gctcgagtcg
     5701 cccttcgcgg ccgccgaccc ggccttcggc cgcgcccgag ggacgatggc ggccctgcgg
     5761 ggcatggccg acagggagac gatcgcctct tgcctctcct acctctggta cgaggccggc
     5821 tatcgcgccg ccctcctctc cagccccggc ggccgcgcct tcgaggagca cttcgagctc
     5881 gtgcactccc tggccgtcgc cgcagacgcc gagggcaggc ccctcgccgc cttcgtcgcg
     5941 gacctggagc cactcgtcgg ctcgcccgag cgcctcgacg acgcggagat cccgagggag
     6001 gccgcccggg gcgtacgcct catgaccgtc cacaagtcga agggcctcga gttccccgtc
     6061 gtggtcgtcc cccaggcgaa caacgcggga cggaacggag gggcgccgtc ctgggcctgg
     6121 gacgacgagg cgggcgccac cttcaggctc cccgcgccgg gcggcgaccc gaagaggcgc
     6181 gacaacatct tctacgagcg cgccgcggag gaacgggccg cgcggcagcg cgcggaggcg
     6241 aagcgcctcc tctacgtcgc cctgacccgc gccgagtcgc aggtcctcgt caccgccacc
     6301 gcccccagga acgaggagaa ggagaagccc aagagcttcc gctccttcct ccttcccgcc
     6361 atgggcctcc gcgcgggctc aggccccggc gcggcggctc ccggcgcggc ggctcccggc
     6421 gcggcggccc ccggcgctgc gggcaacggc tcgccgggcg aggtcgccga agccggcggc
     6481 cgcgaggacg aggcggcccg ggcggcggac gcggtcttgg gcgagcccgt ctatgtctcg
     6541 gagctcgaga cgggacggct gcgccgcctc ccttcgggcg tcctggccgg cctcatccgc
     6601 gagcggagcg acgaggacta cgaggccctc ctgggctcgg cgagggccag ggccgcgaag
     6661 cccgcgatgc ccgacgccgc ccccgtcggg cgccgcgccg cgccaaggct ccgcgcggtg
     6721 agcgccatcg ccgccggatg gtccgcctcg cgggcggtgg gagccgaggg caagggcgac
     6781 gcagccgcgc aaagggccga gccgctcgcc gtcgcgccag gcctcgagcg tcccgagggc
     6841 cttccccccg agctctgggg cagcctcgtc cacgccctca tcgagcgcgc cctcatcgag
     6901 cgcgccctcg cggggaccgc ggctccgcgg gaggaggaac tcgagcccag cctccgctcc
     6961 tccctgcagt cggccctccc ctccgaatcg tccctcaagg ctgcgatgga gcgcgcagcc
     7021 aggctcgccg aggtcttcct cggctcggcg ctcggacgcc gggccctcgc cgcgaaggag
     7081 cgcctcgtcg aatggccgct cgccatccgc tacgaggttc cgcaaggcgc tggcgccgcg
     7141 gccgctgccc gacgtgtcgc gaggggccaa gccgacctcg tcgccgtcga ggagggcagg
     7201 gccttcgtcg tcgactacaa gacggacggg gagatcgagg ccgggcgcta cgatctccag
     7261 gtcgcggcct acaggcgggc cgccgaggcc gtcctcggcc tgccgacgga ggcctacatc
     7321 ttctacctcc acggcggagg ccgcgccgtc cccgtcgccg agtccgcgcc tggcctcgcc
     7381 gacgcgccgc tccctgagcc cgaacccttc gagttctcct tcgcgccggc gaccggggag
     7441 gcgcccgcgc atgggcaggc gccggctcac gggcaggcgc cggcgaccgg gcaggagacg
     7501 gcgcggccgc cggctccccg cgaggcgggc ggatgagcgg caggaaaagc ggcgggggct
     7561 caagcccggc tccgcgcgag ggttcgagcc aggcgccggc cgaagccttg agctccgcgg
     7621 agctccagga cgcctacgag gaccagcgcc tcctcgtcga gatcggccgc gagctcatcg
     7681 tggagaagga ccagggccgc ctcctcgggc gcatcctcga cgcctgccgc ggcatcacgg
     7741 gcgcggacgc gggctcgatc ttcctctgcg agcaaggcga ccgcggcccc ctcctccgct
     7801 tcaagcacgc gaagaccttc tcgcggcccc tggcctacga ggagttcacg atgccccgcg
     7861 acgagcgctc gatcgcgggc tacgtctccc tctcggggaa gccgctcaac gtgcccgacg
     7921 tctacggact cgaccccgcc ctgccctacc gcttcaactc gggctacgac cgctcctcgg
     7981 gctaccgcac gaagagcatg ctggtcgtcc cgatgcgggg cctcaagggc gaggtgatcg
     8041 gcgttatcca gctcatcaac tccaaggaga gcccggaggc cgagtccgag gaggccgtct
     8101 ccggccgcga gatcgccgac tccgtcctcc ttgcggggcc cgaggacttc gagcggctgg
     8161 tcttcccctt caaggagcgc tacgagggcc tcatggaggc ggtcgccaac caggcggcca
     8221 tagccctgga gaacgcccgg atggtgcgcc gcctggccga gcagttcgag tccttcgtcc
     8281 gcgcctccgt cgccgccgtc gagtccctgg accccgcgac gagcggccac tccgagcgcg
     8341 tcgcgaggag cgccgtcgcc ctcatggcgg ccgtggacgc ggagaaggac ggacgctacg
     8401 gcgcctgctc cttcggcgcg gaggagcggc tcgagctcga gtacgcgggc ctcctccacg
     8461 acttcggcaa ggtctacatc gatccgagga tcttcacgaa ggcgaagaag ctcttcgagc
     8521 gggactacga ctgcctcgtg tggcggctca agtactcggg cgccagggcc gagctcgctt
     8581 gcgcgaggcg cgaggtggag gccctccgcg ggggccgcgg ggccgaggcg gagggcgagc
     8641 ggagggaggc cgagcgcgcc aaggcggccg tcctcgaggc cctgggcctc gtccgcgccc
     8701 tgaacgagcc cgaggccacg gagggcgagc ccgaggagag gctcgccctc ctcctcgtcg
     8761 ccgcgccgcc cgcgggcctc gacctggacg gcagccccct ccccctcctc accgaggccg
     8821 agcggcgcga cctttcgatc cgccggggca ccctcaacga ggaggaaaga cggctcatcc
     8881 aggaccacgt gcgctacacc caggccttcg tgagccggat cacctggcct ccggagctgg
     8941 ccggcgtccc cgagtactgc gcgaagcacc acgagatgct cgacggctcg ggatatcccg
     9001 ccgccctcaa ggccgaccgc atccccctcc aggcgcggat gctcgcgatc gccgacgtct
     9061 acgacgccct ggccgcccgc gaccgcccct acaagaaggc cctgcccttc gagaccgtcc
     9121 gctccatcat ggaggaggag gcagccaagg ggcgcctgga cgaggagctc cttcgcctct
     9181 tcttcgagcg ggagtgctgg aacgaaagcg cctccgggcc ggcctgagcg gcaggccgag
     9241 cctttcaccc cgcctggatc cggcggcccg ggatgcctgc ttgggcggcg catattcgca
     9301 agcgcggctc agacccaggc gtcgctcaag ggcccgcaag cgcaacccgc ccgaggggat
     9361 gcccaaggat ctccatttat gtatactgag ggcc
//
LOCUS       DAOOSJ010000087         9292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4765_length_9292_cov_5.915882, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000087 DAOOSJ010000000
VERSION     DAOOSJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4765_length_9292_cov_5.915882"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLG14_05770"
     CDS             <1..580
                     /locus_tag="PLG14_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOX48678.1"
                     /translation="QVPQGSYTVTVRAPGYRDYTQNVLVSGPVQVNAILQANVAAVTI
                     TSNVQGADVVINEGLPLAQRGKTPLTVNLPLGNPPYTIKVSAPGYQDFFQTLAVSGPV
                     SVNAVLQPLAASFQVAVPEAYVNKDLKGGHWSQIMIYVDGVLQRGATGGQLLPGRHVI
                     RVTSGGMAVETVVEAQAGRTYVLEPFMGVSVK"
     gene            739..>1199
                     /locus_tag="PLG14_05775"
     CDS             739..>1199
                     /locus_tag="PLG14_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48679.1"
                     /translation="MIDIREYEQLALDSGAYRDIEIDILKETLASWKARPGDPYTILE
                     LRDGRVLAGFAVFCRAPNTEFTYDILSLCVDPVYRGKGVGRRLLEMLEEEALGSDPAA
                     ILRCETSRKKQEAIGAEVLEDCGYALIGHIADFYEGGDDYFMYARHASRPLA"
     assembly_gap    1200..1299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1300..2133
                     /locus_tag="PLG14_05780"
     CDS             <1300..2133
                     /locus_tag="PLG14_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOX48680.1"
                     /translation="SPPAVLAKTSIPTTSPEETLHAVAAFFEGQAKIHGRPDVAGIGS
                     FGPVELKPSSPAWGRVTSTPKPGWSGADIAGFFMKSLGVAVAFDTDVNAAAYGEYLWG
                     SARGLKDFVYLTVGTGIGGGVFSGGGLVHGLSHPELGHLRLAREADDPFPGLCPYHKD
                     CLEGLASGPAIEARWGKPGRELPEGHPAWELEARYLARAVAAYACVLSPERVLLGGGV
                     GMRPSLAERVAVLLGEELSGYLPALSDPVRLAAYVARPGLGADAGLFGSAAIAMRMPE
                     D"
     gene            2215..3468
                     /locus_tag="PLG14_05785"
     CDS             2215..3468
                     /locus_tag="PLG14_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX48681.1"
                     /translation="MDSFALTDATLVTPRKIRTKATLLVEGGKVAGFGRGAPRSAPVG
                     DGSYVYPALINVHDHLRGNYLPRVGPKGDAFYVNWAPWDNDLKSSKTYEERANLSVEQ
                     MYFLGAYKNLFSGVATTNDHFPHEFNEPFIDRLPIRVIREYCLAHECSSYDLKWGDGI
                     EIEHARAAERDWPFITHLEEGFDPEAQDGVGILERAKCLTDRALLIHCIGFSDEDIRK
                     VKKAEANVAWCPASNLFMFNVTCKIRKLLRAGVNVSLGTDSTHTGSVNLLSEMKCARK
                     VYRDLYGEDLPAKTLVDMVTLNPAKAFRMEGRAGALETGMLADIAVFKAKADEPYESL
                     CRAESRDLELLVLAGEPVYGDEARFGALFEEALAADGGKRFGRIEVAGRRMFVKGDPA
                     ALYRGVRKAVGFEKVLDYLPFEVKA"
     gene            3479..4555
                     /locus_tag="PLG14_05790"
     CDS             3479..4555
                     /locus_tag="PLG14_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48682.1"
                     /translation="MPKVGNYRANSVIYFQGDVSDRIFVLQSGKVSLNYQDIENGKDI
                     HEIIQTGEFFGVKSALGKYPREENALVLADAQVLVFSVPEFEVFAQANTRIIMKMLKV
                     FSNQLRRVHKQVENLLEKSEAESPEAGLFKAGEYYLKNRQYASAKYVFSRYLTYYPAG
                     RLAEKASRYLEAAESASVKYGDGKGPAPLMMEGSRAAGGRQAPGREEEEPVRPAAPAR
                     PQRIPGEDLEGAAKDYYDAKSLFSQEKYKDALVGFKRLVESNADPEYTEKALYDMGRC
                     LFMLQQYDVTIRHFTQLVQTYPKHPDLVDTLYWLGQSYEKKGDKERATGFYKKILAME
                     TDEDASVRLRAQKALRALEEAARG"
     gene            4548..5192
                     /locus_tag="PLG14_05795"
     CDS             4548..5192
                     /locus_tag="PLG14_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX48683.1"
                     /translation="MADLSSFTRFARSYQPGEMIFAEYEPGDSFYLIQAGRVKISKIV
                     GDIEKTIDILQPPEIFGEMAILEGAPRSATAQAVDTVKVLEFNRANFEVLMMGNPQIA
                     LRLLKLFTKRIWDQKRRFMILTLEDPQAKIADVFLMLAETSAAIGAGEQDGEKREFKV
                     TIDDVAHWAGIGGQEAQTVLGHFANQRRVELFEDRIVVRNISDFSRFVNSRRKK"
     assembly_gap    5474..5573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5574..>6634)
                     /locus_tag="PLG14_05800"
     CDS             complement(<5574..>6634)
                     /locus_tag="PLG14_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48684.1"
                     /translation="SHDATYARVANSKLEAILSEASELAQSISELGREVRELGDGRLL
                     DLVQALHGRASMLYRNILETRTIPFGEVAERFRRAVLDIARESGKALRFELSGADTEI
                     DKALADRLAEPLLHLVRNAADHGVERPEARLAAGKPREGSVALRARRESGVLVVRVED
                     DGRGVELEAIRSRAAAYGVPQAEVDSLDEQGLLSLLFRPGFSLSGSVTKWSGRGVGLD
                     AVERNVRAFRGSVRLETRPGRGFAAEIHLPLALSLVEGFTARAGETSLLVPFEAVRSC
                     VSFEDPGDSPSPFRNLPVGGRLVPSIDLSLLYGSPTRAAVRIVALVEAGGSEAGLVLD
                     EVGEALSAAVRPIDRRYADS"
     assembly_gap    6635..6734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6735..7142)
                     /locus_tag="PLG14_05805"
     CDS             complement(<6735..7142)
                     /locus_tag="PLG14_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOX48685.1"
                     /translation="MSDEILAAFAAESNEILEGIEDSIRGLEGGEAEALGSLFRLVHT
                     LKGSASIVGLSRLESFAHAWETRLGKLRSGVAAYGPACAGAFLACRDRVARLLESARP
                     AIGSERGEEGGLRPEDEAVLLSLDAAMAASAAEA"
     gene            complement(7139..8047)
                     /locus_tag="PLG14_05810"
     CDS             complement(7139..8047)
                     /locus_tag="PLG14_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOX48686.1"
                     /translation="MSEVDFSQIVDTISLGILVIDPEGRVVAWNRWMENATGRAYPEV
                     RGRSLYELFPELRGPVFQRNLRSVLQFGNFAYFSQRVHGYLLPMRPDPDSTAAGAERM
                     EQHCVMGPVREGGKIVQVYLVIEDVTEAVHREKLLAELAMKDVLTEAYNRRYFDRRLV
                     EELDRCRRYGRSLGLVMLDLDHFKEVNDLHGHLFGDETLRRSVKAWASVLRSSDILAR
                     YGGEEFCVLLPETELPEARLLAERVRAATSAAEVEFGGRVARITASAGVAASRPGDGP
                     DDILRRVDEALYRAKAGGRNRVEAAT"
     gene            complement(8044..8682)
                     /locus_tag="PLG14_05815"
     CDS             complement(8044..8682)
                     /locus_tag="PLG14_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOX48687.1"
                     /translation="MSDGVEFSALELDALQETMNVGFGQAAASLSEVIDMQVVLSVPK
                     VAMIANEGIAAFVRSEIQDPAAFCMVEQFFFGRFSGTSFLLLPENEGRKLVDLFGAQP
                     DPEFAGESLDALERDIVVEIGNIIIGACVGKIAEMLKDVVCYHPPRYLAGPVDQELIN
                     RHIRPGGGLALVFKTVFKFERQDVLGFLFLVTGDDTASWLKKAVDEMLEGLL"
     gene            complement(8679..9056)
                     /locus_tag="PLG14_05820"
     CDS             complement(8679..9056)
                     /locus_tag="PLG14_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48688.1"
                     /translation="MTGNVLIVDDSLVARLSLKGILKETGLSLSEAPSGEAALEALAA
                     GPPPALVFLDLTMPGLGGIETLRAIRDRFAGLPVVVVTADIQAKTLEAVRELGAFAIV
                     RKPADKAEILGAAERAIGGGAAS"
BASE COUNT         1456 a         3265 c         3013 g         1258 t
ORIGIN      
        1 ccaggtgccc cagggctcgt acacggtcac cgtccgcgcc cccggctacc gcgactacac
       61 ccagaacgtc ctcgtgagcg gccctgtcca ggtgaacgcc atcctccagg ccaacgtcgc
      121 cgccgtgacg ataacctcca acgtacaggg agccgacgtc gtcatcaacg agggcctgcc
      181 cctcgcccag cgcggcaaga cccccctcac cgtcaatctt cccctcggga atccccccta
      241 tacgatcaag gtgagcgcgc ccggctacca ggatttcttc cagactctcg ccgtgagcgg
      301 ccccgtctcc gtcaacgcgg tcctccagcc cctggcggcc tccttccagg tcgccgtccc
      361 cgaggcctac gtcaacaagg acctaaaggg cgggcactgg agccagatca tgatctacgt
      421 cgacggcgtc ctgcagcgcg gggcgacggg cgggcagctc ctccccggcc gccacgtgat
      481 ccgcgtgacc tcgggcggca tggccgtgga gaccgtcgtc gaggcccagg ccggcaggac
      541 ctacgtgctc gagcccttca tgggcgtcag cgtcaaatag ggcccgcgcg cccggaaaga
      601 gacgcggccc cgccggaaca tcagccggcg gggctgtttt ttagccgatt tcgcgcgatt
      661 cctgtacaaa tcgggacgcg ggaactatac tcgggcggag cccagccggg ccgcagccac
      721 gcgacaaggt caggaacgat gatcgacatc cgggagtacg aacagcttgc cctcgattcc
      781 ggcgcctacc gggacatcga gatagacatc ctcaaggaga ccctcgcgtc ctggaaggcg
      841 aggcccggcg acccctacac gatcctggag ctccgcgacg gccgcgtcct cgcgggcttc
      901 gcggtcttct gcagggcgcc caacaccgaa ttcacctacg acatcctctc cctctgcgtc
      961 gatccggtct accgggggaa gggcgtcggc cggcgcctcc tggagatgct ggaggaggag
     1021 gccctgggct ccgaccccgc cgccatcctc cgctgcgaga cctcccgcaa gaagcaggag
     1081 gccatcggcg ccgaggtcct ggaggattgc ggctacgccc tgatcggcca catcgccgac
     1141 ttctacgagg gcggcgacga ctacttcatg tacgcgcggc acgcgagccg gcccttggcn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgccccccgc cgtcctcgcc
     1321 aagacgagca tcccgaccac gagccccgag gagaccctcc acgccgtcgc ggccttcttc
     1381 gaaggccagg caaagatcca cggccggccc gacgtcgcgg gcatcggctc cttcgggccg
     1441 gtggagctca agccctcgag cccggcctgg ggccgcgtca cctccacccc caagccgggc
     1501 tggagcgggg ccgacatagc cggcttcttc atgaaaagcc tgggcgtcgc cgtcgccttc
     1561 gacacggacg tgaacgcggc cgcctacggc gagtacctct ggggctcggc ccgcggcctc
     1621 aaggacttcg tctacctcac cgtcggcacc ggcatcggcg gcggggtctt ctccgggggc
     1681 ggcctcgtgc acggcctctc ccaccccgag ctcggccacc tgaggctcgc ccgcgaggcc
     1741 gacgatccct tccccggcct ctgcccctac cacaaggact gccttgaggg cctcgcctcc
     1801 ggccccgcca tcgaggcgcg ctggggcaag cccggccgcg agctccccga gggccatccc
     1861 gcctgggagc tcgaggcccg ctacctggcc cgggccgtcg cggcctacgc ctgcgtcctc
     1921 tcgccagagc gcgtcctcct gggcggcggg gtcggcatgc ggccgagcct ggccgaaagg
     1981 gtggccgtcc tcctcgggga ggagctctca ggctacctgc cggccctctc cgaccccgtc
     2041 cgcctggccg cctacgtcgc caggccgggc ctgggggccg acgccggcct cttcggctcg
     2101 gccgccatag ccatgcggat gccggaggat tgagggagag gcgattcccg ctgtactttt
     2161 ccggcgcttg agccgaaaat taagagaagt cgaacccgcc aaggatgggc aaccgtggac
     2221 tctttcgcgt taaccgacgc cacgctcgtg acaccccgca agatccgcac caaggcgacc
     2281 ctcctcgtcg agggagggaa ggtcgcgggc ttcgggcgcg gcgcgcccag gtcggcgccg
     2341 gtcggcgacg gctcctacgt ctatcccgcc ctcatcaacg tccacgacca cctccgcggc
     2401 aactacctgc cccgcgtcgg cccgaagggc gacgccttct acgtcaactg ggccccctgg
     2461 gacaacgacc tcaagtcctc caagacctac gaggagcggg ccaacctctc cgtcgagcag
     2521 atgtacttcc tcggcgccta caagaacctc ttctccggcg tcgcgacgac gaacgaccac
     2581 ttcccccacg agttcaacga gcccttcatc gaccgcctgc ccatccgcgt gatccgcgag
     2641 tactgcctgg cccacgagtg ctcctcctac gacctcaagt ggggcgacgg gatcgagatc
     2701 gagcacgccc gcgccgcgga gcgcgactgg cccttcatca cccacctgga ggagggcttc
     2761 gaccccgagg cccaggacgg cgtcggaatc ctggagcgcg cgaagtgcct cacggaccgg
     2821 gccctcctca tccactgcat cggcttctcc gacgaggaca tccgcaaggt gaagaaggcc
     2881 gaggccaacg tggcctggtg ccccgcctcg aacctcttca tgttcaacgt cacctgcaag
     2941 atccggaagc tcctcagggc cggcgtcaac gtctccctgg gcaccgactc gacccacacg
     3001 ggctcggtga acctcctttc ggagatgaag tgcgcgcgca aggtctaccg ggacctctac
     3061 ggcgaggacc tgcccgcgaa gaccctcgtc gacatggtga ccctgaaccc cgccaaggcc
     3121 ttccgcatgg aaggccgcgc gggcgccctg gagacgggca tgctcgccga catcgccgtg
     3181 ttcaaggcca aggccgacga gccctacgag agcctctgcc gcgccgagag ccgggacctc
     3241 gagctcctcg tgctggcggg cgagccggtc tacggcgacg aggcccgctt cggcgccctc
     3301 ttcgaggagg ccctggccgc cgacggcggc aagcgcttcg gcaggataga ggtggcgggc
     3361 cgccgcatgt tcgtcaaggg agaccccgcc gcgctctatc gcggcgtcag gaaggcggta
     3421 ggcttcgaga aggtcctcga ctacctcccc ttcgaagtca aggcatagga gcgcggcggt
     3481 gccgaaggtc ggaaactacc gggccaactc ggtcatatac ttccaggggg acgtctccga
     3541 ccggatcttc gtcctccagt cgggcaaggt gagcctcaac taccaggaca tcgagaacgg
     3601 caaggacatc cacgagatca tccagacggg cgagttcttc ggcgtgaaga gcgccctcgg
     3661 gaagtacccc cgcgaggaga acgccctcgt gctggccgac gcccaggtcc tcgtgttcag
     3721 cgtgcccgag ttcgaggtct tcgcccaggc gaacacccgc atcatcatga agatgctcaa
     3781 ggtcttctcc aaccagctca ggcgcgtgca caagcaggtc gagaacctcc tcgagaagtc
     3841 cgaggcggag agccccgagg ccggcctctt caaggcgggc gagtactacc tcaagaaccg
     3901 ccagtacgcc tccgcgaagt acgtcttcag ccgctacctc acctactacc ccgccggccg
     3961 cctggcggag aaggcgagcc gctacctgga ggcggcggag tccgcctccg tgaagtacgg
     4021 cgacggcaag ggccccgccc ccctcatgat ggagggctcg cgggccgcgg gaggccgcca
     4081 ggcccccggg cgcgaggagg aggagccggt ccgcccggcc gcccccgccc gcccccagcg
     4141 gatccccggc gaggacctgg agggcgccgc caaggactac tacgacgcga agagcctctt
     4201 cagccaggag aagtacaagg acgccctggt cggcttcaag cgcctcgtcg agtcgaacgc
     4261 cgatcccgag tacaccgaga aagcgctcta cgacatgggc aggtgcctct tcatgctcca
     4321 gcagtacgac gtgaccatcc gccacttcac ccagctcgtc cagacctatc ccaagcatcc
     4381 cgacctcgtc gacaccctct actggctggg ccagtcctac gagaagaagg gcgacaagga
     4441 gagggcgacg ggcttctaca agaagatcct ggcgatggag acggacgagg acgcctccgt
     4501 gcgcctccgt gcgcagaagg cgctccgcgc cctcgaggag gccgcccgtg gctgacctct
     4561 cctccttcac ccgcttcgcc cgctcctacc agcccggcga gatgatcttc gccgagtacg
     4621 agccggggga cagcttctac ctcatccagg ccggccgcgt gaagatctcc aagatcgtcg
     4681 gcgacatcga gaagaccatc gacatcctcc agccccccga gatcttcggg gagatggcca
     4741 tcctggaggg tgcgccccgc tccgcgaccg cccaggccgt cgacacggtg aaggtcctcg
     4801 agttcaaccg cgcgaacttc gaggtcctca tgatgggaaa cccgcagatc gccctacggc
     4861 tcctcaagct cttcacgaag cgcatctggg accagaagcg ccgcttcatg atcctcaccc
     4921 tggaggatcc gcaggcgaag atcgccgacg tcttcctcat gctcgcggag acgagcgccg
     4981 ccatcggcgc cggcgagcag gacggcgaga agcgcgagtt caaggtgacg atcgacgacg
     5041 tggcccactg ggccggcatc ggcggccagg aggcccagac cgtcctcggc cacttcgcga
     5101 accagcgccg cgtcgagctc ttcgaggacc gcatcgtggt gcggaacatc tcggacttca
     5161 gccgcttcgt gaactcccgc aggaagaagt aagctcggcc gccccttccc aatcagactc
     5221 ggacgacgcc gaagagcgat cgcggcgggt ttcgcggtac ttgcccgggc cacccgccgc
     5281 ccagccgctc cccgcgagac cgtaggcggg ttctttcgcc ttcacgccgc gatcggcgga
     5341 agggatggcc accgagtgat gcttcctccc gcggcgaaac gggggacctc cgaggcaccc
     5401 cgcccgcgtc ccgcatagcg ccgcatacaa cgtcaccctt aggcgatctc cccaccccgc
     5461 gccgtccggg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagtcgg
     5581 cgtagcggcg gtcgatgggg cggacggcgg cggagagggc ctcgcccact tcgtccagga
     5641 ccaggccagc ctcggagccg ccggcctcga ccagggcgac gatgcggacg gcggcgcgtg
     5701 tgggagagcc gtagaggagg gagaggtcga tggaggggac gaggcggccg cccacgggca
     5761 ggttccggaa ggggctcggc gagtcgcccg ggtcctcgaa ggagacgcag gagcgcaccg
     5821 cctcgaaggg cacgaggagg gaggtctcgc cggcgcgggc ggtgaagccc tcgaccaggg
     5881 agagggccag gggcaggtgg atctccgcgg cgaagccccg gcccggcctg gtctccaaac
     5941 gcaccgagcc gcggaaggcg cggacgttgc gctccaccgc gtccaggccc acgcctcggc
     6001 ccgaccactt ggtcacggag cccgagaggg agaagccggg ccggaagagg agggagagga
     6061 ggccctgctc gtccagggag tcgacctccg cctgcggcac gccgtaggcg gcggcgcggg
     6121 acctgatggc ctcgagctcc acgccgcggc cgtcgtcctc gacccggacg acgaggacgc
     6181 ccgactcccg ccgggcccgc agggcgacgc tgccctcccg cggcttgccc gccgcgagcc
     6241 gcgcctcggg acgctcgacg ccgtggtcgg ccgcgttccg caccaggtgg aggaggggct
     6301 cggccagtcg gtccgccagg gccttgtcga tctcggtgtc ggccccggag agctcgaagc
     6361 gcagggcctt gccgctctcc ctcgcgatgt ccaggacggc ccgcctgaag cgctcggcga
     6421 cctcgccgaa ggggatcgtc ctcgtctcca ggatgttgcg gtagagcatg gaggcccggc
     6481 cgtgcagggc ctggaccagg tcgaggaggc ggccgtcgcc gagctcccgc acctcgcgcc
     6541 cgagctcgga gatcgactgg gcgagttcgg aggcctcgga caggatggcc tccagcttcg
     6601 agttcgcgac gcgggcgtag gtcgcgtcgt gggannnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnncgcctc agccgccgaa gcggccatcg cggcgtccag cgacaggagg
     6781 acggcctcgt cctcgggcct gaggcccccc tcctctcccc tctcggagcc gatggcgggc
     6841 cgggcggact cgaggaggcg ggcgacgcgg tcccggcagg ccaggaaggc gccggcgcag
     6901 gcggggccgt aggcggcgac gccggagcgg agcttcccga gcctcgtctc ccaggcgtgg
     6961 gcgaaggact cgagccgcga gaggccgacg atgctcgccg agcccttcag ggtgtggacg
     7021 aggcggaaga gggagcccag ggcctcggcc tcgcccccct ccaggccgcg gatggagtcc
     7081 tcgatgccct cgaggatctc gttggactcg gcggcgaagg ccgcgaggat ctcgtcgctc
     7141 acgtggccgc ctccacgcgg ttcctgccgc cggccttggc ccggtagagg gcctcgtcca
     7201 cccgcctcag gatgtcgtcg gggccgtcgc cgggccgcga ggccgcgacg cccgcgctcg
     7261 ccgtgatccg cgcgacgcgg ccgccgaact cgacctccgc cgcggaggtc gccgcgcgca
     7321 cgcgctccgc gaggagcctc gcctccggca gctcggtctc cgggaggagg acgcagaact
     7381 cctcgccgcc gtagcgggcc aggatgtcgg aggagcgcag gacggaggcc caggccttga
     7441 cggagcggcg cagggtctcg tccccgaaga ggtggccgtg gaggtcgttc acctccttga
     7501 agtggtccag gtcgagcatc acgaggccga ggctccgccc gtagcggcgg cagcggtcca
     7561 gctcctccac gaggcgccgg tcgaagtagc ggcggttgta ggcctccgtg aggacgtcct
     7621 tcatcgcgag ctccgccagg agcttctcgc ggtggaccgc ctccgtcacg tcctcgatca
     7681 cgaggtagac ctggacgatc ttgccgccct cgcggacggg ccccatcacg cagtgctgct
     7741 ccatgcgctc ggcccccgcg gccgtggagt cgggatcggg ccgcatgggg aggaggtagc
     7801 cgtggacgcg ctgcgagaaa tacgcgaagt ttccgaactg caggacgctc cggaggttgc
     7861 gctggaagac ggggccgcgg agctccggga agagctcgta gagggagcgg ccccgcacct
     7921 cgggataggc gcggccggtc gcgttctcca tccagcggtt ccaggcgacg acgcggccct
     7981 cgggatcgat cacgaggatc ccgagggaga tggtgtccac gatctgggag aagtcgacct
     8041 cgctcacagg agcccttcga gcatctcgtc cacggccttc ttgagccagg aggcggtgtc
     8101 gtcgccggtc acgaggaaga ggaagccgag gacgtcctgc ctctcgaact tgaagacggt
     8161 cttgaacacg agggccaggc cgccgccggg gcggatgtgg cggttgatga gctcctggtc
     8221 cacgggcccc gcgaggtagc ggggcggatg gtagcagacc acgtccttga gcatctccgc
     8281 gatcttcccg acgcaggcgc cgatgatgat gttgccgatc tccacgacga tgtcgcgctc
     8341 cagcgcgtcc aggctctcgc ccgcgaattc cgggtcgggc tgggcgccga agaggtcgac
     8401 gagcttccgg ccctcgttct ccgggaggag gaggaagctc gtgcccgaga agcggccgaa
     8461 gaagaactgc tcgaccatgc agaaggccgc gggatcctgg atctcggagc gcacgaaggc
     8521 cgcgatgccc tcgttggcga tcatcgcgac cttgggcacg ctcaacacga cctgcatgtc
     8581 gatgacctcg gacagcgagg ccgcggcctg gccgaagccg acgttcatcg tctcctggag
     8641 ggcgtccagc tcgagggccg agaactcgac cccgtcgctc acgatgccgc tcccccgccg
     8701 atcgcgcgct ccgcggcgcc caggatctcc gccttgtccg cgggcttgcg gacgatggcg
     8761 aaggcgccga gctcccgcac ggcctccagg gtcttggcct ggatgtccgc ggtgaccacc
     8821 acgaccggaa gccccgcgaa gcggtcccgg atcgccctga gcgtctcgat gccccccagg
     8881 cccggcatgg tcaggtcgag gaagacgagg gcgggcgggg gccccgccgc gagggcctcg
     8941 agcgccgcct cgccgcttgg cgcctccgag agggagaggc cggtttcctt gaggatgccc
     9001 ttgagcgaga ggcgggcgac gagcgagtcg tcgacaatga gcacgtttcc ggtcatgaat
     9061 cgagtatagc ctgcgcctcc ccgaacggca atacgagccg cctccgcgcg cctcgcctcg
     9121 cgccgcgcct gcccctgcct ctcccgccgc ggccgcgccg cccccccgcg cccccatcct
     9181 cttgacccgc cgccttcccc tcggctatag tcccgatgcg tgccgagata gctcatttgg
     9241 cagagcggcg cactcgtaat gcgcaggtgt cgggttcgat tcccgatctc gg
//
LOCUS       DAOOSJ010000088         9273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4773_length_9273_cov_5.164678, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000088 DAOOSJ010000000
VERSION     DAOOSJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_9273_cov_5.164678"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLG14_05825"
     CDS             complement(<1..601)
                     /locus_tag="PLG14_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOX48689.1"
                     /translation="MKEGFLSRLSRLARGQGKPAEAQAERGDEGEEAKAERREMIKGV
                     QELAETTVREVMVPRIDTVFVPLDVGREELLEKVSSSGHSRFPVYRESIDEVVGMLYA
                     KDLLRALARGGDFSLSDIVRKPFFVPESKRIDSLLREMKRRRVHIAVAVDEYGGVSGI
                     VSLEDIIEEIVGDIQDEFDDEPEDLSSLGEGVWLCDARAD"
     gene            complement(598..1053)
                     /gene="ybeY"
                     /locus_tag="PLG14_05830"
     CDS             complement(598..1053)
                     /gene="ybeY"
                     /locus_tag="PLG14_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOX48690.1"
                     /translation="MNDVEISLEGVEEKPWIEEVSAFALAALERLGKDNWDLSLLFCD
                     DAFIQGLNRQYRDKDEPTDVLSFEQGEVYRGPDGKERVLAGDIVISLGGLARNVEEFR
                     VPEAEELRRLVLHGILHLSGMDHEDNDPSRPMLALQEELLSSLRKAGGR"
     gene            complement(1040..3256)
                     /locus_tag="PLG14_05835"
     CDS             complement(1040..3256)
                     /locus_tag="PLG14_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOX48691.1"
                     /translation="MKPNSLSLAGLRAVLAGRSGDRGLLFSLAGTFAAGLLLVLFLGP
                     GAAISRSALDEIEVGRVAEKDIVADRELTFVDDKATALRVEAEERLVLPIYVVDEKVG
                     VAALERFRVFQELLVSLSAKKYAEDSLYLKVQSEFPGLLSKDDVVSLARYPLKSQAFS
                     HADAVLRRLLERGVAAAPSAGLERYNPDYVELRRWRDGRLEYEELPASRLPSLRASAG
                     VIDDEIVARRLSRPLSNYVASLVRSFLVENAFFDADQSARRLESVRRRVDPVVRTIAR
                     GERIVRKGFIISESDYAKLAAAKAAVSRADWGRLAGDAGFFVVVFALALLLLGPAVSG
                     AALRGGEALLAAGGGAVYLAAALVISRFAGFSDSLDLALLLPTALFAMLFAIIVGQRF
                     ALLYGLILALLALLASGFDARPFALAFLASAAGAFSVRQAGSRIELVRAGGLLALLEA
                     GAAFLLAAPGARGAAELLLPAAFGAANGFLCGVLTLGFLPVIEQALNAPTRFRLMELS
                     DLNAPILKRLLTVAPGTYSHSVTVAHLAESACREIGADPLLARVGAYYHDIGKIEQPE
                     YFVENQAGYNKHDELNPRLSATVIRSHVKLGVERARSLGLPEEVVDIVAEHHGNGLIA
                     WFYNQAAKGDAEAKAEDFSYPGRPPASREAAVVMLADTVEAATRTLKKPTLARLDQAI
                     KDLMMDKVRQGQLDRSDLTFRDLETIRNTFVRILAGHFHSRIEYPKLAREARDERR"
     gene            complement(3243..4214)
                     /locus_tag="PLG14_05840"
     CDS             complement(3243..4214)
                     /locus_tag="PLG14_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOX48692.1"
                     /translation="MDGISGIVLENTGTLADVCGANDYNLRIMERLLGSRVYSRGNEL
                     FLESEDAATRELFRALLERLVESLEEGMPPSPDLIIAIHASLTPEGEGGPRACADGPS
                     EFLDSCVQIPGGTGKVYPRSRSQALYLKGLATHDLCFSVGPAGTGKTYLAVAWALREI
                     LQKSKRKLILTRPVVEAGESLGFLPGDLAQKISPYLRPLYDAMEALVPYEVVRRLEES
                     RAIEIAPLAYMRGRSLSNCVVILDEAQNTTKEQMKMFLTRLGENSRAVVTGDVTQIDL
                     PRKSESGLVHALGLLKSVDGVFVCRLDSRDVVRSGLVRRIIEAYEAE"
     gene            complement(4359..7925)
                     /locus_tag="PLG14_05845"
     CDS             complement(4359..7925)
                     /locus_tag="PLG14_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48693.1"
                     /translation="MRRDPGQTRYAGALLLALILPFARLGAQAGQPPASGAAAPSGGA
                     ASTPAGDAAAEAPPSADPPSGAASSAEDEADLVRKTLALDISTASFYELVAWCRSRGL
                     PSGGSIAELKARLYESYGVEPPAAAGAAGRTVTIERAAEAEYFELEDEGSSLVRLSGG
                     VVLVVREEGKAESHRLEADEILYDRDRNSVSAKGRVRYRREGSAGVEEFFGETIVVDL
                     DDWSGVFLDGRLRRTEAGAAEGERGLSFSSETMLKRSGDVIILERGTVSSCDEESPHY
                     SVRARKMWLLGENEWAVSDAVLSVGEVPILWLPFFYYPGDEIVFHPVFGYRSREGRFV
                     QTTTYLIGRKVEKKEASGFFKMAKASSGGPVETRGIFLHPVKDAEAAAKDAASGPTLK
                     LLADLYSGLGAFVGLDGKLAAKAPLEKLEFTAGVGLSRSLFPVTNGYSPYVQEGDWRS
                     VMNGSELFGASLPFRYGLDLSASLRSGGLSASLGLPVYSDPFFERDFRYRSEDMEWLD
                     FGGLADSASSTSTSASSSQPSKRSSLLQRLDLSYSWRPAALSPWLSQFDLSRLGLSLT
                     WLAKAATDDGSILYDADPAREFFYPDVFRPVDASLTLRGSLLPAKGKEAAGAAASVAA
                     QGEGLREPWEEASGGAAGEGEAAGGEGEGEGPAGPADAIGGPAGGAAGEGGAESGVAA
                     AAPKNEATGSGLRQAGRLPDAPRAASRDPFSLSADWSLTPSAFIEDRYLGGEWQESGD
                     IDFSRLYRLSSYRLGATLGATARAFGDLATASLGLSFNSQDQSRVSTDDPDYASRVAS
                     YRLADAQYRQAKLGSSLKLSAKPFADSWLFGSSSLSYSLDAKLYELAYKGMDGDTALY
                     SERFPEWSSDGVTSHAAAFTLGLRTGSKTQSLGVAMSLPPTTEAYSALLSLEAGEGGL
                     GATLGVKARMYRPTGAADYSYDPVSATLGLTGSGGLKLGDSLSYDAVLGKPLTNSASL
                     GLGGFTATLQHKQSPGYTPVLGSGWVSDGDTAFRATDFTAALKGDLKSPEDSKLGLGL
                     SLSGSYTQNLLRFSESVLSLGLTASLKIDRFLDLSFTSLSQNAAVWRYWPGLFPAVDA
                     LGGAEAWYKNPLLDLYESVSFWDPEARRRSLFKLKSLSLKLKHDLHDWDLSFELSATP
                     KLDTSSTPYVYVLDTSFSILVAWRDVPEIKTKVTKDDSGLAY"
     gene            complement(8059..>9273)
                     /locus_tag="PLG14_05850"
     CDS             complement(8059..>9273)
                     /locus_tag="PLG14_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48694.1"
                     /translation="DIGNTLIVVEHDEQTLRTADYIVDLGPGAGVHGGHVVAQGSPDE
                     VMRNRKSVTGQYLAGSISIDVPAVRREGSGKSISIKGCREHNLKGIDVEIPLGGFTCI
                     TGVSGSGKSTLLTDLLYPVISNRVMGTAWPEGEYGSIEGLEAIDKIINIDQSPIGRTP
                     RSNPATYVGVFTAIRDLFASLPESKKRGWKPGRFSFNVKGGRCEECQGDGTKKIEMNF
                     LPDVYITCEVCHGRRFNADTLDARYKGKNIADVLDMTIEEAAGFFEHIPPIAHKMRTL
                     LSVGLGYIKLGQSALTLSGGEAQRVKLAFELAKRSTGRTLYFMDEPTTGLHFADVKQL
                     MEVIQRLVDTGNSVVMIEHNLDVIKQADWIIDLGPEGGARGGTVVAKGRPEDIARVKA
                     SYTGRYLKDALK"
BASE COUNT         1382 a         2991 c         3545 g         1355 t
ORIGIN      
        1 ggtcggcccg cgcgtcgcag agccagacgc cctcgcccag ggaggagagg tcctcgggct
       61 cgtcgtcgaa ctcgtcctgg atgtcgccga ctatctcctc gatgatgtcc tccaggctca
      121 cgatgccgga gacgccgccg tactcgtcca cggcgaccgc gatgtggacg cggcgccgct
      181 tcatctcgcg gaggagggag tcgatgcgct tggactcggg cacgaagaag ggctttcgca
      241 cgatgtccga gagcgagaag tcgccgccgc gcgccagggc ccggagcagg tccttcgcgt
      301 agagcatgcc gaccacctcg tcgatgctct cgcggtagac ggggaagcgc gagtggccgg
      361 aggaggagac cttctccagg agctcctccc gcccgacatc gagggggacg aagaccgtgt
      421 cgatgcgcgg gaccatgacc tcgcgcaccg tcgtctcggc gagctcctgg acgcccttga
      481 tcatctcgcg gcgctcggcc ttcgcctcct cgccctcgtc gccgcgctcg gcctgggcct
      541 ccgcgggctt gccctgcccc cgcgcgaggc gcgagaggcg cgagaggaag ccttccttca
      601 tcgtccgccc gccttccgca gggaggacag gagctcctcc tggagggcca gcatgggccg
      661 agaggggtcg ttgtcctcgt ggtccatgcc ggataggtgg aggatgccgt ggaggaccag
      721 gcgcctgagc tcctcggcct cgggcacgcg gaattcctcc acgttgcgcg ccagtccccc
      781 gagggagatc acgatgtcgc ccgccaggac gcgctccttc ccgtcggggc cccggtagac
      841 ctcgccctgc tcgaaggata ggacgtccgt gggctcgtcc ttgtcgcggt actggcggtt
      901 cagcccctgg atgaaggcgt cgtcgcagaa gaggagggag aggtcccagt tgtccttgcc
      961 gagccgctcg agggcggcca gggcgaaggc ggagacctcc tcgatccagg gcttctcctc
     1021 cacgccttcg agggagatct caacgtcgtt catcgcgcgc ctccctggcc agcttcggat
     1081 actcgatccg ggaatggaag tgccccgcga ggatccggac gaaggtgttg cggatcgtct
     1141 ccaggtcccg gaaggtgagg tcggagcggt ccagctggcc ctgcctgacc ttgtccatca
     1201 tgaggtcctt gatcgcctgg tcgagcctgg cgagggtcgg cttcttgagg gtccgagtgg
     1261 cggcctccac ggtgtcggcg agcatcacga cggcggcctc gcggctcgcg gggggcctgc
     1321 cagggtagct gaagtcctcg gccttggcct cggcgtcgcc cttcgcggcc tggttgtaga
     1381 accaggcgat gaggccgttg ccgtggtgct ccgcgacgat gtccacgacc tcctccggaa
     1441 ggccgaggct ccgcgcccgc tcgacgccga gcttcacgtg gctccggatc acggtcgcgg
     1501 agaggcgggg gttgagctcg tcgtgcttgt tgtagcccgc ctggttctcc acgaagtact
     1561 cgggctgctc gatcttcccg atgtcgtggt agtaggcgcc gacgcgcgcg agcaggggat
     1621 cggcgccgat ctcgcggcag gccgactcgg ccaggtgggc gacggtcacg gagtggctgt
     1681 aggtgcccgg ggcgacggtg aggaggcgct tgaggatggg cgcgttgagg tcggagagct
     1741 ccatgaggcg gaagcgggtc ggagcgttca gggcctgctc gatgacgggg aggaagccca
     1801 gcgtgaggac gccgcagagg aagccgttcg ccgcgccgaa ggccgctggc aggaggagct
     1861 ccgccgcgcc ccgggcgccg ggcgccgcga ggaggaaggc ggcccccgcc tcaaggaggg
     1921 cgagaaggcc gcccgcgcgg acgagctcga tgcgggagcc ggcctggcgg acggagaagg
     1981 ccccggccgc cgaggcgagg aaggccaggg cgaagggcct cgcgtcgaag cccgaggcga
     2041 ggagggcgag gagggcgagg atgaggccgt agaggagggc gaagcgctgc ccgacgatga
     2101 tggcgaagag catcgcgaag agggctgtcg ggaggaggag ggccaggtcc agggagtcgg
     2161 agaagcccgc gaagcgcgag atcaccaggg cagccgcgag gtagacggcg ccgcccccgg
     2221 ccgcgaggag ggcctcgccg ccgcggaggg cggcgcccga gacggcgggg ccgaggagga
     2281 ggagggccag ggcgaagacg acgacgaaga agcccgcgtc tcccgcgagc ctgccccagt
     2341 cggcgcggga gacggccgcc ttggccgcgg cgagcttcgc gtagtcgctc tccgagatta
     2401 tgaagccctt ccgcacgatc cgctcgcccc gcgcgatggt gcgcaccacg gggtcgaccc
     2461 gccggcgcac cgactcgagg cggcgcgcgc tctggtcggc gtcgaagaag gcgttctcca
     2521 cgaggaagct gcggaccagg gaggcgacgt agttcgagag ggggcgggag aggcgccggg
     2581 cgacgatctc gtcgtcgatg acgccggcgg aggcgcggag gctcggcagg cggctcgcgg
     2641 gcagctcctc gtactcgagg cggccgtccc gccagcggcg gagctcgacg tagtcggggt
     2701 tgtagcgctc gaggccggcg gagggcgcgg cggcgacgcc ccgctccagg aggcggcgga
     2761 gcacggcgtc ggcgtgggag aaggcctggg acttgagggg atagcgggcg agggagacca
     2821 cgtcgtcctt ggagaggagg ccggggaatt cgctctggac cttgaggtag aggctgtcct
     2881 ccgcgtactt cttcgcggag agggagacga ggagctcctg gaagacgcgg aagcgctcca
     2941 gggccgccac gccgaccttc tcgtcgacga cgtagatcgg gaggacgagg cgctcctcgg
     3001 cctcgacgcg gagggccgtc gccttgtcgt cgacgaaggt gagctcgcgg tcggcgacga
     3061 tgtccttctc cgcgacgcgg ccgacctcga tctcgtccag ggcgctccgg gagatggccg
     3121 cgccggggcc gaggaagagg acgaggagga gtccggcggc gaaggtcccg gcgagggaga
     3181 aaaggaggcc gcggtcgccc gagcgtcccg cgaggacggc gcggaggccg gcgagggaaa
     3241 ggctattcgg cttcataggc ttcgatgatc ctccggacga ggccgctccg caccacgtcc
     3301 cgcgagtcca ggcggcagac gaagacgccg tccaccgact tgaggagtcc gagggcgtgc
     3361 acgaggcccg actcggactt gcgcgggagg tcgatctggg tgacgtcgcc cgtcacgacg
     3421 gcgcggctgt tctcgccgag ccgggtgagg aacatcttca tctgctcctt ggtcgtattc
     3481 tgcgcctcgt ccaagatgac gacgcagttc gagaggcttc ggccccgcat gtaggccagg
     3541 ggcgcgatct ctatggcgcg gctctcctcc aggcggcgga cgacctcgta ggggacgagg
     3601 gcctccatcg cgtcgtagag gggccggagg tagggactga tcttctgggc caggtcgccg
     3661 ggcaggaagc cgaggctctc tcccgcctcc acgaccggcc tggtgaggat gagcttgcgc
     3721 ttggacttct ggaggatctc gcggagggcc caggccacgg cgaggtaggt cttccccgtg
     3781 ccggcgggcc cgacggagaa gcagaggtcg tgggtggcga ggcccttcag gtagagggcc
     3841 tggctgcggg agcggggata gaccttgccc gtccccccgg ggatctggac gcaggagtcg
     3901 aggaactccg aggggccgtc ggcgcaggcc ctggggccgc cctcgccctc gggcgtgagg
     3961 ctcgcgtgga tggcgatgat gaggtcggga gaggggggca tgccctcctc cagggactcg
     4021 accaggcgct cgaggagggc ccggaagagc tcgcgcgtcg ccgcgtcctc gctctccagg
     4081 aagagctcgt tcccgcggct gtagacgcgg ctcccgagga gccgctccat gatgcggaga
     4141 ttgtagtcgt tcgctccgca aacgtcggcc agggtgccgg tgttctcgag cacgatgcct
     4201 gaaatgccgt ccaaaggggc ctctccgcgt tactttacag gcccttccgg aaaaaatcaa
     4261 caagcgggcc gggccagccg ggcgcccggc gcggccggga ggcggcgggc ttgtccctcc
     4321 ggccacccca ccggccgcca ggcggcgcgg gtcgcggcct agtaggcgag cccggaatcg
     4381 tccttcgtca ccttggtctt gatctcgggc acgtcccgcc aggcgacgag gatcgagaat
     4441 gaggtgtcga ggacgtagac gtagggcgtc gagctcgtgt cgagcttggg cgtggccgag
     4501 agctcgaagg agaggtccca gtcgtggagg tcgtgcttga gcttcaggga gaggctcttc
     4561 agcttgaaga ggctcctgcg cctggcctcg ggatcccaga aggagacgga ctcgtagagg
     4621 tcgaggaggg ggttcttgta ccaggcctcc gcgccgccca gggcgtccac ggcgggaaag
     4681 aggcccggcc agtagcgcca gacggcggcg ttctgggaga ggctcgtgaa ggagaggtcg
     4741 aggaagcggt cgatcttgag gctggccgtc agccccaggg agaggacgga ctccgagaag
     4801 cggaggagat tctgcgtgta ggaaccgctc agggaaaggc cgaggcccag cttcgagtcc
     4861 tccgggctct tgaggtcgcc cttcagggcg gcggtgaagt cggtcgcgcg gaaggccgtg
     4921 tcgccgtcgg agacccagcc cgagccgagg acgggcgtgt agccggggct ctgcttgtgc
     4981 tggagggtcg cggtgaagcc gcccaggccg aggctcgcgg aattggtcag gggcttcccc
     5041 agtacggcgt cgtaggagag gctgtcgccc agcttgaggc cgcccgaccc cgtgaggccc
     5101 agggtcgcgg agacggggtc gtaggaatag tccgccgccc ccgtcggcct gtacatgcgc
     5161 gccttgactc ccagggtggc gcccaggccg ccctcccccg cctccaggga gaggagggcg
     5221 ctgtaggcct ccgtggtcgg ggggaggctc atcgcgacgc ccaggctctg ggtcttcgag
     5281 cccgtgcgca ggcccagggt gaaggcggcc gcgtggctcg tgacgccgtc ggagctccat
     5341 tcggggaagc gctccgagta gagggccgtg tcgccgtcca tgcccttgta ggcgagctcg
     5401 tagagcttgg cgtccaggct gtagctcaag gagctcgagc cgaagagcca ggagtcggcg
     5461 aagggcttgg cggagagctt gaggctcgag ccgagcttgg cctggcggta ctgcgcgtcc
     5521 gcgaggcggt aggaggcgac gcgcgaggcg tagtcggggt cgtcggtcga gacgcgcgac
     5581 tggtcctggg agttgaagga aaggccgagg gaggccgtcg ccaggtcgcc gaaggccctc
     5641 gccgtcgcgc cgagcgtggc tccgagccgg taggaggaaa gccggtagag gcggctgaag
     5701 tcgatgtccc cggactcctg ccattccccg cccaggtagc ggtcctcgat gaaggccgag
     5761 ggcgtgaggc tccagtccgc ggagagggag aagggatcgc gggaggcggc ccggggcgcg
     5821 tccggaagcc gcccggcctg gcggagcccc gagcccgtgg cttcattctt tggagcggcg
     5881 gccgccacgc cggactccgc gccgccctcc cccgccgcgc ctccggccgg gccgccgatc
     5941 gcgtcggccg ggcctgccgg cccctcgccc tctccctcgc ctcccgcggc ctcgccttcc
     6001 ccggcggccc cgcccgaggc ctcctcccag ggctcccgca gcccctctcc ttgggcggcg
     6061 acggaggcgg cggcgcccgc ggcttccttg cccttcgcgg gcaggaggct tcccctgagc
     6121 gtgaggctcg cgtccacggg gcggaagacg tcggggtaga agaactcccg cgccggatcg
     6181 gcgtcgtaga gtatgctccc gtcgtcggtc gcggccttgg cgagccaggt gagggagagg
     6241 cccaggcggc tcaggtcgaa ctgcgagagc caggggctca gggcggccgg ccgccaggaa
     6301 taggagagat cgaggcgctg gaggagggag ctccgcttgc tcggctggct ggaggaggcc
     6361 gaggtcgagg tcgaggaagc cgaatccgcg aggcccccga aatcgagcca ctccatgtcc
     6421 tcggaacggt agcggaaatc gcgctcgaag aagggatcgg agtagacggg aaggccgagg
     6481 ctcgcggaga gtcctcccga gcgcagggag gccgacaggt ccaggccgta gcggaagggc
     6541 aggctcgcgc cgaagagctc cgagccgttc atcacgctcc gccagtcgcc ctcctggacg
     6601 tagggcgaat agccgttcgt gacggggaag agggagcggg aaaggccgac gcccgcggtg
     6661 aactcgagct tctccagggg ggccttcgcc gcgagctttc cgtccaggcc gacgaaggcg
     6721 cccaggcccg aatagaggtc ggcgaggagc ttgagggtcg gaccggaggc cgcgtccttg
     6781 gcggccgcct ccgcgtcctt cacgggatgg aggaagatgc cccgcgtctc cacgggcccg
     6841 cctgacgagg ccttggccat cttgaagaag ccgctcgcct ccttcttctc caccttcctc
     6901 ccgatgaggt aggtggtcgt ctggacgaag cgcccctcgc gggagcggta gccgaagacg
     6961 ggatggaaga cgatctcgtc ccccggataa tagaagaagg ggagccagag gataggcacc
     7021 tcgccgacgg agaggacggc gtccgagacg gcccactcgt tctcgcccag gagccacatc
     7081 ttgcgggccc gcaccgagta gtggggggac tcctcgtcgc aggagctcac cgtgccgcgc
     7141 tccaggatga tgacgtcgcc cgagcgcttg agcatggtct ccgaggagaa ggaaaggccc
     7201 cgctcgccct ccgcggcgcc cgcttccgtc ctgcggagcc ggccgtccag gaagacgccg
     7261 gaccagtcgt ccaggtcgac gactatcgtc tccccgaaga attcctcgac gccggccgac
     7321 ccctcgcggc ggtagcgaac ccggcccttg gccgagacgg agttgcggtc gcggtcgtag
     7381 aggatctcgt cggcctccag gcggtgcgac tcggccttgc cctcctcgcg gacgaccagg
     7441 acgacgccgc cggaaagcct gacgagggag cttccctcat cctcgagctc gaagtactcg
     7501 gcctccgcgg cgcgctcgat cgtgacggtc cggcccgcgg ctccggccgc cgcggggggc
     7561 tcgacgccgt agctctcgta gagccgggcc ttgagctcgg cgatggagcc gcccgagggg
     7621 aggcccctcg acctgcacca ggccacgagt tcgtagaagg aggcggtgga aatgtcgagg
     7681 gcgagagtct tgcggacgag gtcggcctcg tcctcagccg aggaggcggc tccggaaggc
     7741 gggtccgcgg agggcggcgc ctcggcggca gcgtcgccgg ccggcgtgga cgccgcgccg
     7801 cctgagggcg cggcggctcc cgaggcgggc ggctgccccg cctgggcgcc gaggcgcgcg
     7861 aaggggagga tgagggctag gaggagagct cccgcgtatc gcgtctggcc tgggtcgcgg
     7921 cgcaccaggc catcatagcg cgggcccgcc cggctcgtaa agccgaaagc cgccggcgcg
     7981 gccggggcgc ggccttcaag cgaagcccgc cgcccggcgc gccgccccgg gaccaggcgc
     8041 gcgtctggcg gctccggcct acttgagggc gtccttcagg tacctgcccg tgtaggaggc
     8101 cttcacgcgg gctatgtcct cgggccggcc cttcgcgacg accgtgccgc cgcgggcgcc
     8161 gccctcgggg cccaggtcga tgatccaatc cgcctgcttg atcacgtcca ggttgtgctc
     8221 gatcatcacg accgagttcc ccgtgtccac gaggcgctgg atcacctcca tgagctgctt
     8281 cacgtcggcg aagtgcaggc ccgtcgtcgg ctcgtccatg aagtagagcg tgcggcccgt
     8341 gctccgcttc gcgagctcga aggcgagctt cacgcgctgg gcctcgccgc ccgacagggt
     8401 cagggcggac tggccgagct tgatgtagcc gaggcccacg gagaggaggg tcctcatctt
     8461 gtgggcgatc ggggggatgt gctcgaagaa gcccgcggcc tcctcgatcg tcatgtccag
     8521 gacgtcggcg atgttcttgc ccttatagcg ggcgtccagg gtgtcggcgt tgaagcgccg
     8581 gccgtggcag acctcgcagg tgatgtagac gtcggggagg aagttcatct cgatcttctt
     8641 cgtgccgtcg ccctggcact cctcgcagcg gccgcccttc acgttgaagc tgaagcgccc
     8701 cggcttccag ccgcgcttct tcgactcggg gagggatgcg aagaggtcgc ggatcgcggt
     8761 gaagacgccg acgtaggtcg cggggttgga gcggggcgtc cggccgatcg ggctctggtc
     8821 gatgttgatg atcttgtcga tcgcctccag gccctcgatg gagccgtact cgccctccgg
     8881 ccaggcggtg cccatgacgc ggttcgagat cacggggtag aggaggtccg tgaggagggt
     8941 gctcttcccg gagcccgaga cgccggtgat gcaggtgaag ccgccgagcg ggatctccac
     9001 gtcgatgccc ttgaggttgt gctcgcggca gcccttgatg gagatggact tgcctgagcc
     9061 ctcgcggcgg acggcgggca cgtcgatgga gatgctcccc gccaggtact ggcccgtgac
     9121 gctcttcctg ttccgcatca cctcgtcggg gctgccctgc gcgacgacgt ggccgccgtg
     9181 gacgccggct cccggcccca ggtcgacgat gtagtccgcg gtgcggaggg tctgctcgtc
     9241 gtgctcgacg acgatgaggg tgttgccgat gtc
//
LOCUS       DAOOSJ010000089         9246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4790_length_9246_cov_5.172016, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000089 DAOOSJ010000000
VERSION     DAOOSJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_9246_cov_5.172016"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLG14_05855"
     CDS             complement(<1..635)
                     /locus_tag="PLG14_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOX48695.1"
                     /translation="MKPSLLAAALCCAAASAWAVGAAPAASPPASSQPAAAAASAFLE
                     ESPLATVEFLDGKVTVTRDGEPLKGVSIGDELYDEDLLSTGADGSLVLALAPATGMRG
                     TIRVSPKSSFYLRSEPSGGGQKSQAELIAGQLSLKMKKLAGEPSFEVTSGSTVMGVRG
                     TEFTVTSSPSGALLVSCVEGEVACVEDGDAASAVPGQAVERREGARLRRLAV"
     gene            complement(713..961)
                     /locus_tag="PLG14_05860"
     CDS             complement(713..961)
                     /locus_tag="PLG14_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48696.1"
                     /translation="MTTTKHAERRSKQRGIGEDLIDLAADYGRLEGDRIILGAQAIGE
                     LLEEVERYRRMLLRARDKGGLAIVLRDERLITTFPLWR"
     gene            1118..2605
                     /gene="radA"
                     /locus_tag="PLG14_05865"
     CDS             1118..2605
                     /gene="radA"
                     /locus_tag="PLG14_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOX48697.1"
                     /translation="MSAKKTATVFRCANCGRVEPRWLGRCPECGEWNSLREASAAEAR
                     PDRAGETFPMPIGAIDPGRGARIPTGSSEVDRVLGGGLMRGSAVLLGGEPGIGKSTLL
                     LQICAKAELQGRILYVSGEESPAQIRLRADRLGALREGIEVFSSGDLARIRGVLETVK
                     PVLVVIDSVQTLHAEEAGAVPGTANQIKYCVQELVSWAKEREAGLFLVAHVTKEGLIA
                     GPKAAEHLVDAVLSFEQSEAELRTLRASKNRFGSAEELGIFRMGEKGLEEVADPSGLF
                     LVRREGGLPAGVAVAPVHEGSRVLLVEIQALAVPAKAGLSRVYSERIDSGRVARVAAV
                     LEKHTGMRFSDQDLYVNVAGGIRLAEPGIDLPLAAALYSARSGTPLPDGSALAGELSL
                     AGELRPVRQMRRRARAAKALGLDLVLGPAGGEEGEGKEWRVARDIREALKALLGGKSG
                     AAAGADSGGSAGGSAGGSAGSAAASRSRARSGGPRAGRDGEGGEA"
     gene            2605..3285
                     /locus_tag="PLG14_05870"
     CDS             2605..3285
                     /locus_tag="PLG14_05870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOX48698.1"
                     /translation="MLYLLRHGQTAPDGVFTDDGQPDPALTPLGEAQARAAAEALAGE
                     GLSAVFSSDLRRAVATAGAVASLCGLEPRTDRRLREIDMGEAGRLGGEEFARTHPEAA
                     RELEASRRLGEASRDFRYPGGETGGEAAARAEAFIADLSGDRASMGAGDRVLLVCHGG
                     LIRALVARLLGMEERRRFRLMPGNCGVTRLELPGRAAGAAEGPNLLDRARLLSFNEEG
                     HLRGMTSF"
     assembly_gap    3287..3386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3486..5591
                     /locus_tag="PLG14_05875"
     CDS             3486..5591
                     /locus_tag="PLG14_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOX48699.1"
                     /translation="MNYAHNGRRVSGLVAALSALRSRESPACGEYPDLAALGGLARAW
                     GLDLIQLLPVNDSGSQSSPYSALSAFALHPLYLRVADLPEAAGKAGAPYRKAAAELAA
                     RFADDARLRYGPYLEAKLALLERIYAERGAAALGLPGAAPGAGARVPAGAEAGAASEA
                     GAIQAFIAANPWVKAYAVFSELKRRYEGKPWWEWTERRDPEPAEVEALWEEPSLAAGT
                     RFRAWLQWRAEGQFAAAAAALREAGVELMGDIPILISEDSAEVWARRRVFRLGLAAGA
                     PPDMYSHLGQNWGFPIYDWEALEREGYGFWKERLASCEKFYSAYRIDHVLGFFRIWAL
                     SGRDRTGYLGRFVPDAHIGRAELEALGFGPDRLRWLSEPHVRTERLVAACSAGGAGDA
                     DAMEAALLALDRIGNEELFLFKRSIRGEKDIEELAARGAAGYGGAGVSEGARAFLLEA
                     WRDRVLYEFEPGRYAPAWRHSEASAWGSLSGGEREALESLFAAKRAEGERIWEETGRR
                     LLGVLKAESGMLPCAEDLGSVPPCVPRVLEELGILGLRVLRWTRDWGRPGDPFTPLGD
                     YPELSVACPSVHDSSSLREWWETEADRGLVWDLAGRTLGKDLGAAPARLDPAAAAVLL
                     KAMAASASRIVVYPFQDLLALGEAFRPADPKAERVNVPGSANEWNWGWRLPAALEEIA
                     ADRGLQAAVAELAAARAMA"
     gene            5615..7291
                     /locus_tag="PLG14_05880"
     CDS             5615..7291
                     /locus_tag="PLG14_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX48700.1"
                     /translation="MAGAIRASGMAMGALPDAGAESLLEDYVGAGIRAFLLPGSLLQR
                     PERLAALAALARRRASEAGLGRALVALGGGAVPAAAPPPLPGLPGPLGLAAAGERGLA
                     RRTGRLLGGLLAVLGVDLVLAPRLDLATDPKAPGGILDLFGEDSLRAGAMAAAYARGM
                     AEGGAAACGLSFPGCGRLVSDGRKGPPLVSYPLERLGGIEALPFARAVKAGLPAVLVA
                     RVLAPALEPERLPAARSARVVEGRLRTDLGFKGLVLGGALDEDPEGPGRAATLGALAG
                     CDLSLALSPAAALEAARALDKALASGELPAPRAVVAGRRLEALFSRLPARDAASPADP
                     AAAGAAARALLEGAQAARLGERAARRAATAFRAAPPRGPALRAGQPRAGEAQATTAAS
                     GAGGAVEPAPRPRGFPLSAPPRLVLVMLPPEGGPEAAQSPGFLAALGEALPGARVLSL
                     PCDPGPAEAELLAAAAEAAAPGPALALTCDAHRRPGQESLVHLLEESLPEVGVVALGD
                     PYDAAFFPRAAALGAVYGRDASAARAAAGLALGTLEARGRCPVLVLGLEV"
     gene            7343..8305
                     /locus_tag="PLG14_05885"
     CDS             7343..8305
                     /locus_tag="PLG14_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOX48701.1"
                     /translation="MDSETSPGPKRATEAALRGAKSAVGTTWFLAKILVPVTLAVALL
                     GWSGVLSLLSRLLSPAMGLLGLPGEAALVLVSALFLNIYSAIAVAGTLALDLREVAVL
                     AIMCLTAHNLLVETPVMRKSGSSGTKMVALRLLAALAAGFAYSLILPAGLAATPFSAG
                     APAARPEFAAMLGAWGLSTLRLVAKIALIVLAVSVAQRLLEEFRIMDLLSRLFAPLMR
                     FLGLPAKASFLWIVINVVGYAYGAGIVVAEIESGRMKPQDGDLFNHHAAVCHSLLEDT
                     VLFLALGLPLFWLTLPRLAMAAVVVWAERARRHYFRRSFRAGIG"
     gene            complement(8617..>9246)
                     /locus_tag="PLG14_05890"
     CDS             complement(8617..>9246)
                     /locus_tag="PLG14_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOX48702.1"
                     /translation="FDLLKVLNLEARFHPSKAPAGPAGAFVLGLAFGAGWSPCVGPVL
                     ASILLLAAREGSLAKSALLLGAYSLGLALPFVAAGLFLERLTPLMAWFKRRAHAVRIA
                     SGLLLLALGAFMALGRLAAVSALAARAGFALKEALVASPDALRLGAAAAWALLATLVL
                     ALPALRKKPVLTAPRIAFAALFALAAAAEASGLLSTARLLSEWLLYSGG"
BASE COUNT         1105 a         3439 c         3357 g         1245 t
ORIGIN      
        1 acggccaggc gcctgagccg cgcgccctcg cgacgctcga cggcctggcc gggaacggcc
       61 gaggccgcgt cgccgtcctc cacgcaggcc acctcgcctt cgacgcagga cacgaggagg
      121 gcgcccgagg gcgagcttgt cacggtgaat tcggtgccgc ggacgcccat caccgtcgag
      181 ccgctcgtga cctcgaagga gggctcgccc gcgagcttct tcatcttgag gcttagctgg
      241 ccggcgatga gctcggcctg gctcttctgg ccgccgcctg agggctccga gcggaggtag
      301 aaggaggact tgggagagac cctgatcgtg ccgcgcatcc ccgtggcggg ggcgagggcc
      361 aggacgaggc tgccgtcggc gcccgtcgag aggagatcct cgtcgtaaag ctcgtccccg
      421 atggagacgc ccttgagcgg ctcgccgtcg cgggtgactg taaccttgcc gtccaggaat
      481 tcgacggtcg cgagggggct ctcctcgagg aaggccgacg cggcggccgc ggccggctgg
      541 gacgaagccg gcggggaggc ggctggggcg gctccgacgg cccaggccga ggctgcggcg
      601 cagcaaagag cggccgcgag aagcgatggt ttcatgatgg ctcctatggc ctaggaacaa
      661 ccttcccgcg cggtgattac ggcgaatcct cgcagggcgc ggacggaggc gctcaacgcc
      721 agagcgggaa ggtcgtgatg aggcgctcgt cgcgcaagac gatggcgagg ccgcccttgt
      781 cccgggccct gagcagcatc ctgcggtagc gctcgacttc ctcaaggagc tcgcctatgg
      841 cctgggcgcc caggatgatg cggtcgccct ccagcctccc gtagtccgcg gcgaggtcga
      901 tgaggtcctc gcctatgccg cgctgcttgg aacgcctttc ggcgtgcttg gtggtcgtca
      961 tgtccgcctc cggtcttcgc caaaggaagg gctttcctga ctgcagttta tagtatatgt
     1021 ccccgcggcc caattcgcaa agccgcgccc cttcgcgggc gggcaggccc cgcgccgccg
     1081 ttgaatcgcc agagcgcctc ggctacgata ggcccatatg agcgcgaaaa agacggcgac
     1141 cgtgttccgc tgcgcgaact gcggcagggt ggagccccgc tggctcgggc gctgccccga
     1201 gtgcggggag tggaacagcc tccgcgaggc ctcggccgca gaggcgcggc ccgaccgcgc
     1261 gggcgagacc ttccccatgc cgatcggcgc gatcgatccg gggcgggggg ccaggatccc
     1321 gacgggctcg agcgaggtgg accgcgtcct gggggggggc ctcatgcggg gctcggccgt
     1381 cctcctgggc ggggagccgg ggatcggcaa gtcgaccctc ctcctccaga tctgcgcgaa
     1441 ggccgagctc cagggccgca tcctctacgt ctccggcgag gagtcgcccg cgcagatccg
     1501 cctcagggcc gaccgcctgg gcgccctccg cgagggcatc gaggtcttct cctcgggcga
     1561 cctggcccgg atccgcggcg tcctggagac cgtgaagccc gtcctcgtcg tcatcgactc
     1621 ggtccagacc ctccacgccg aggaggccgg cgccgtgccc ggcacggcca accagatcaa
     1681 gtactgcgtc caggagctcg tctcctgggc gaaggagcgg gaggcggggc tcttcctcgt
     1741 cgcccacgtg acgaaggagg gccttatcgc cgggcccaag gcggccgagc acctcgtgga
     1801 cgccgtcctc tccttcgagc agagcgaggc ggagctccgg accttgcgcg cctcgaagaa
     1861 ccgcttcgga agcgccgagg aactcggcat cttccggatg ggggagaagg gcctggagga
     1921 ggtcgccgac ccctcgggcc tcttcctcgt gcgccgcgag ggaggcctgc cggccggcgt
     1981 cgccgtcgcg ccggtccacg aggggagccg cgtcctcctc gtcgagatcc aggccctggc
     2041 cgtgcccgcg aaggccgggc tctcccgggt ctattcggag cgcatcgact cagggagggt
     2101 cgcgcgggtc gctgccgtcc tcgagaagca cacggggatg cgcttctccg accaggacct
     2161 ctacgtcaac gtcgcgggcg gcatcaggct cgccgagccg ggtatcgacc tgcccctggc
     2221 cgccgccctc tactcggccc gctccgggac tcccctgccg gacgggagcg ccctcgcggg
     2281 cgagctttcc ctcgcggggg agctcaggcc cgtcaggcag atgcggcgcc gcgccagggc
     2341 cgcgaaggcc ctgggcctcg accttgtcct cgggcccgcg ggcggcgagg agggggaggg
     2401 gaaggagtgg cgcgtcgcgc gggacatcag ggaggcgctc aaggccctcc tcggggggaa
     2461 gtccggcgcc gcggccggcg cggattcggg cggctccgct ggcggctcgg cgggcggctc
     2521 cgcggggagc gccgcggcga gccggagccg cgcgcgatcg ggcggcccgc gtgccgggag
     2581 ggacggcgag ggaggcgaag cctgatcctc tatctcctcc gccacgggca gacggcccct
     2641 gacggcgtct tcaccgatga cggccagccc gatcccgcgc tcacgcccct aggcgaggcc
     2701 caggccaggg cggccgctga ggccctcgcg ggcgaggggc tctccgcagt cttctcgagc
     2761 gacttaaggc gggccgtcgc gacggccggg gcggtcgctt ccctttgcgg cctcgagcct
     2821 cggaccgacc gccgcctccg cgagatagac atgggcgagg cgggcaggct cggcggagag
     2881 gagttcgcga ggacccatcc cgaggctgcc cgcgagctcg aggcctcgag gaggctgggc
     2941 gaggcctccc gcgatttccg ctatccgggc ggggagacgg gaggggaggc ggccgcccgc
     3001 gcggaggcct tcatcgccga cctgtccggg gaccgagcct cgatgggcgc gggggaccga
     3061 gtcctcctcg tctgccacgg cggcctcatc cgcgccctcg tcgccaggct gctcgggatg
     3121 gaggagcgtc gccgcttccg cctcatgccg ggcaactgcg gcgtcacgag gctcgagctg
     3181 ccggggcgag cggcgggggc ggccgagggg ccgaacctcc tggaccgggc gcggctcctc
     3241 tccttcaacg aagagggcca cctccgcggg atgacgagct tctaggnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnngcgc ctcggctccg gcgccgcggc cattcccgcg
     3421 gccattcccg cggccattcc cgcggcgcgc gggccgggct atactcgcaa cgtaggggga
     3481 ctcccatgaa ctacgcccac aacggccgcc gcgtcagcgg cctcgtcgcc gccctttcgg
     3541 ccctgcggag ccgcgaaagc cccgcctgcg gcgagtatcc cgacctcgcg gccctgggcg
     3601 gcctcgcccg cgcctggggc ctggacctca tccagctcct ccccgtcaac gactcgggct
     3661 cccagagctc tccctactcg gccctctcgg ccttcgccct ccaccccctc taccttcggg
     3721 tcgccgacct gcccgaggcc gccggcaagg caggcgctcc ctacaggaag gccgcggccg
     3781 aactggccgc ccgtttcgcg gacgacgcga ggctccgcta cggcccctac ctcgaggcca
     3841 agctcgctct cctcgagcgg atctacgccg aacggggcgc ggccgcgctc ggcctgcccg
     3901 gagcggcacc tggggccggc gcgcgcgtgc ctgccggcgc ggaagccggc gcggcttccg
     3961 aggccggggc catccaggcc ttcatcgcgg cgaatccctg ggtgaaggcc tatgcggtct
     4021 tctccgagct caagcggcgc tacgagggga agccctggtg ggagtggacc gagcgccgcg
     4081 atcccgagcc ggccgaggtc gaggcgctct gggaggagcc ttcgctcgcg gcgggcacgc
     4141 gcttccgggc ctggctccag tggcgcgccg aggggcagtt cgcggccgcg gccgcggccc
     4201 tgcgcgaggc gggcgtggag ctcatgggcg acatccccat cctcatcagc gaggactccg
     4261 cggaggtctg ggcgaggagg cgggtcttcc gcctgggctt ggccgccggc gccccgccgg
     4321 acatgtactc ccacctgggc cagaactggg gcttccccat ctacgactgg gaggccctgg
     4381 agcgcgaggg ctacggcttc tggaaggagc gcctggcctc ctgcgaaaag ttctattccg
     4441 cctatcgcat cgaccacgtc ctgggcttct tccgcatctg ggccctctca ggccgcgacc
     4501 ggacgggcta cctgggccgc ttcgtccccg acgcccacat cgggcgggcg gagctcgagg
     4561 ccctgggctt cggccccgac cgcctgcgct ggctctccga gccccacgtc cgcacggagc
     4621 gcctcgtcgc ggcctgctcg gccggcgggg ccggcgacgc cgacgcgatg gaggccgccc
     4681 tcctcgccct ggaccggatc gggaacgagg agctcttcct tttcaagcgc tccatccggg
     4741 gcgagaagga catcgaggag ctcgcggccc gcggagcggc gggctacggc ggggccggcg
     4801 tctcggaagg ggcccgcgcc ttcctcctcg aggcctggag ggaccgcgtc ctctacgagt
     4861 tcgagccggg gcgctacgcc cccgcctgga ggcactccga ggcgagcgcc tggggcagcc
     4921 tctccggcgg cgagcgggag gccctggaga gcctcttcgc cgccaagcgg gcggagggcg
     4981 agcggatctg ggaggagacg ggccgccgcc tcctgggcgt cctcaaggcg gagagcggga
     5041 tgctgccctg cgccgaggac ctgggctccg tgccgccctg cgtgccccgc gtcctcgagg
     5101 agctcggcat cctgggcctc cgcgtcctcc gctggacgag ggactggggc aggcccggcg
     5161 accccttcac tccccttggc gactaccccg agctctccgt ggcctgcccc tcggtccacg
     5221 actcctcctc cctgcgcgag tggtgggaga cggaggccga ccgcggcctg gtctgggacc
     5281 tggccggcag gaccctcggc aaggacctgg gcgcggcgcc cgcgaggctg gaccccgcgg
     5341 cggcggccgt cctcctcaag gcgatggccg cctcggcgtc gcgcatcgtc gtctatccct
     5401 tccaggacct cctggccctg ggagaggcct tcaggccggc cgatcccaag gcggagcgcg
     5461 tcaacgtgcc gggctccgcg aacgagtgga actggggctg gcgcctccca gccgccctgg
     5521 aggagatcgc ggccgaccgc ggcctccagg ccgccgtagc cgagctggcg gccgcccggg
     5581 ccatggcgta gaattgaggg cgggaggagg cggcatggca ggcgcgatcc gggcctcggg
     5641 catggcgatg ggggccctcc ccgacgccgg ggccgagtcc ctcctggagg actacgtcgg
     5701 cgcggggatc cgcgccttcc tccttcccgg ctccctcctc cagaggcccg agcgcctcgc
     5761 cgccctggcc gcgctcgcga ggaggcgagc ctccgaggcg ggcctgggcc gcgccctggt
     5821 cgcgttgggc ggcggagccg tcccggccgc cgcgcctccg cccctccccg gcctccccgg
     5881 ccccttgggc ctcgccgccg ccggcgagcg cggccttgcc cggaggacgg gaaggctcct
     5941 gggcgggctc ctcgccgtcc tcggcgtcga cctggtcctc gcgccccgcc tggacctggc
     6001 gacggatccg aaggccccgg ggggcatcct ggacctcttc ggcgaggact ccctgcgcgc
     6061 gggcgcgatg gccgccgcct acgcccgcgg gatggccgag gggggcgcgg ccgcctgcgg
     6121 cctctccttc cccggctgcg gccgcctggt ctcggacggc cgcaaggggc cgcccctcgt
     6181 ctcctacccc ctggagcggc tgggcggcat cgaggccctg cccttcgccc gcgcggtcaa
     6241 ggccggcctt cccgccgtcc tcgtggcccg cgtcctcgcg ccggccctgg agcccgagcg
     6301 cctgcccgcg gcgcgcagcg cccgcgtcgt cgagggccgc ctccgcacgg acctgggctt
     6361 caagggcctg gtcctgggcg gcgccctgga cgaggatccc gaggggccgg ggagggccgc
     6421 gaccttgggc gccctggctg gctgcgacct ctccctggcc ctctcgccgg ccgcggccct
     6481 ggaggcggcc cgcgccctgg acaaggccct cgcctcgggc gagctcccgg cgccgcgggc
     6541 cgtggtcgcg ggacggcggc tcgaggccct cttctcgcgt cttcccgcga gggacgccgc
     6601 ctctcccgcc gatccggcgg ccgccggggc cgccgcccgc gccctcctgg agggggctca
     6661 ggcggcgcgg ctcggcgagc gggcggccag gcgggcggcc acggccttca gggcagcccc
     6721 gccccgaggc ccggcgctcc gggcgggcca gccgcgcgcc ggcgaggcgc aggcgacgac
     6781 ggccgcgagc ggggctggcg gcgccgtcga gccggctccc cggccccgcg gcttcccgct
     6841 ctcggccccg ccccgcctcg tcctcgtcat gcttcccccc gaaggcggcc ccgaggcggc
     6901 ccagtcgccc ggcttcctcg ccgccctggg cgaggccctg cccggcgccc gcgtcctcag
     6961 cctgccctgc gatccgggac ccgccgaggc cgagctcctc gccgcggcgg cggaggccgc
     7021 ggctccgggc ccggccctgg ccctcacctg cgacgcccac cggcgccccg gccaggagag
     7081 cctcgtccac ctcctggaag agagcctgcc cgaagtcggc gtcgtcgccc tcggggatcc
     7141 ctacgacgcg gccttcttcc ccagggcggc cgcgctgggc gcagtctacg gccgcgacgc
     7201 ctccgccgcg agggccgccg ccggcctcgc cctcggaacc ctggaggccc gcggccgctg
     7261 ccccgtcctc gtcctgggcc tcgaggtcta gaaggccgct tccttgacag gcccgccggg
     7321 cggaagctag attcgtctga cgatggatag cgaaacgagc ccgggcccga agcgggcgac
     7381 agaggccgcc ctgcgcggcg cgaagtccgc ggtcggcacg acctggttcc tcgcgaagat
     7441 cctggtgccc gtcaccctcg ccgtggccct cctcggctgg agcggcgtcc tttccctcct
     7501 ctcccgcctc ctttcgccgg ccatgggcct cctcggcctc ccgggagagg cggccctggt
     7561 cctcgtctcc gccctcttcc tcaacatcta cagcgcgata gccgtcgccg gcaccctggc
     7621 cctcgatctc cgggaggtcg ccgtcctcgc gatcatgtgc ctgacggcgc acaatctcct
     7681 cgtcgagacg ccggtgatgc ggaagtccgg ctcctcgggg acgaagatgg tcgcgctccg
     7741 gctcctcgcg gccctggccg ccggcttcgc ctacagcctc atcctgcccg caggcctggc
     7801 cgcgacgccc ttctccgcgg gcgcgcccgc cgcgcggccc gagttcgccg cgatgctcgg
     7861 cgcctggggc ctctccaccc tccggctcgt cgcgaagatc gccctcatcg tcctggccgt
     7921 gtcggtggcc cagcgcctcc ttgaggagtt ccggatcatg gacctcctct ccaggctctt
     7981 cgcccccctc atgcgcttcc tgggcctgcc ggccaaggcg agcttcctgt ggatcgtcat
     8041 caacgtcgtg ggctacgcct acggcgcggg catcgtcgtc gcggagatag agtcggggag
     8101 gatgaagccc caggacggcg acctcttcaa ccaccacgcg gcggtctgcc actccctcct
     8161 cgaggacacg gtcctcttcc tggccctggg cctgcccctc ttctggctca ccctgccccg
     8221 ccttgcgatg gccgccgtgg tggtctgggc ggagcgggcg cggaggcact acttcaggag
     8281 atcgttcagg gcgggcattg gttgaccggg gagaggaacc tttcgtttcg agcgaaaggt
     8341 tcctctcccc ggacccctct cttccaaagg cgcgaccagg gggctccgcc cctggacccc
     8401 gcataatctt gggtgctctg cttttatgcg ccgcacgcgt ccggggcttc gcccggacgg
     8461 cgcatggtgg gtcgcctggc tgaggcttgt cgccgggttg tctaccggat catcgtgaaa
     8521 tcgaaggctg gttgcccacc gcaggctgca tccgcgccgc acgcgtccgg gcttcgcccg
     8581 gacggcgcgt tgggcgtctc gatactatct ttccgctcag ccccctgaat ataagagcca
     8641 ctccgacagg agcctcgccg tcgacagcag gccggaggcc tcggcggcgg cggccagggc
     8701 gaaaagggcg gcgaaggcga tccggggcgc ggtgaggacg ggcttcttgc gcagggccgg
     8761 tagggccagg accagggtgg cgaggagggc ccaggcagcc gcggcgccga ggcgcagggc
     8821 gtccggcgag gcgaccaggg cctccttgag ggcgaagccc gcgcgggcgg ccagggcgga
     8881 gacggcggcg aggcggccca gggccatgaa ggcgcccaag gcgaggagga ggaggccgga
     8941 ggcgatccgc acggcgtggg cccgccgctt gaaccaggcc atgagaggcg ttagccgctc
     9001 caggaagagg cccgccgcca cgaaggggag ggcgaggccc aggctgtatg cccccaggag
     9061 cagggcagac ttagcgaggc tgccctcccg cgccgcgagg aggaggatgg atgcgaggac
     9121 gggaccgacg cagggcgacc agcccgcgcc gaaggccagg cccagcacga aggcgcccgc
     9181 cggacccgcc ggcgccttgg agggatggaa tcgcgcctcc aggttcagga ccttgaggag
     9241 gtcgaa
//
LOCUS       DAOOSJ010000090         9111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4853_length_9111_cov_5.788648, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000090 DAOOSJ010000000
VERSION     DAOOSJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_9111_cov_5.788648"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1224
                     /locus_tag="PLG14_05895"
     CDS             <1..>1224
                     /locus_tag="PLG14_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOX48703.1"
                     /translation="PAGATRPDGVAAAGAGSPAGLDFAALARACLERTEALVGRHGPR
                     LAGGDAGKAAARELAEELRGFCDSVRAEEFEAHPASFYAYMKVLPAAYLLGLLSILAF
                     RGLYLAPLLGLVSGIALMASQFGLYGRLGDRLFPRRTCLNVEGIVEPTGEAEFELIVS
                     GHHDSAPVARIYSGPFQRWYAFAIFAPYPFFLLQAGLLASLIARPGSAPPAWAPAVLL
                     SGLPFAVAYFLLVDTRRGSPGAGDNLVSSVLAATIARELAARKGELLRRTRLRAASFD
                     AEEAGLRGSAAYMRARRGESGGLPVLMLNFDSLYSASELHVLVSDVNGTVRLSRGMAE
                     DAADCLRAEGHDPRLYPMAFGGGATDAAEAARAGVQATTVIAMPTAVVREGLVYHTPR
                     DRVEAIEPGVVEACLR"
     assembly_gap    1225..1324
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1758..2306
                     /locus_tag="PLG14_05900"
     CDS             1758..2306
                     /locus_tag="PLG14_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48704.1"
                     /translation="MEEEFYTDKWGTFLSAYAPIRTEDGTFDGLLCLDISYAQVSKIM
                     LGHLLGLLLLLFGCSALVVPVAVFRARSLTRPIKRVSQAFREISSGEVTDLTRRVEAL
                     DSDEIGAMAEIANGTFEKMRNLVTSIRAQAGAISEVGFELSANMTETAASINQISANI
                     QSVKRLAGNQAQSVAETNHAMD"
     gene            2391..3140
                     /locus_tag="PLG14_05905"
     CDS             2391..3140
                     /locus_tag="PLG14_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48705.1"
                     /translation="MLANIASVTGVLNSDARNVAELAAASEAGRADLASVSERIREVA
                     RESEGLLEISSVIQNVASQTDLLSMNAAIEAAHAGESGKGFAVVADEIRKLAESSGEQ
                     SKTISASLKRIKDSMEVITLSTDKVMDQFEDINSRIRAVSEREKGIRDAMEEQGVGSK
                     EILEAVGKLAEISGEVRSGSEEMLAGSREVIKESASLGRITEEVTGSMAEMAAGAEQI
                     TVAVNKVNDLAKDNKESIDALMAEVAKFKVE"
     gene            complement(3207..3782)
                     /gene="pth"
                     /locus_tag="PLG14_05910"
     CDS             complement(3207..3782)
                     /gene="pth"
                     /locus_tag="PLG14_05910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOX48706.1"
                     /translation="MVRLYAFLGNHGREYRGNRHNVAWQFLESLPFYGELRFERKFKG
                     RFAQWEGPQGRVWLLAPETYMNLSGDSAAELMRFFQIGPDEILAVHDELELPFGVFGF
                     KQGGGLGGHNGLRSLEARLGTRDFSRLRFGIGRPDHPDVAGYVLSDFTKAEREALDCS
                     VFPRAASALEVCLREGFEEAYKRFQKVKALD"
     gene            3875..5692
                     /locus_tag="PLG14_05915"
     CDS             3875..5692
                     /locus_tag="PLG14_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOX48707.1"
                     /translation="MTADPRLALDVDEADGQVRIRLRAAADIQFSEEEPVLLRATVGE
                     LLGLARAPEAFREALLDPRRRLLVANGWQSWSYAGELGPRERVKACLVGRFRPFSQRP
                     GRLERRGELLSHFYVGLRAGEGRLFLVSRNAGTPPLSFRLDRRSLDLSVEALAGGAAF
                     KAGQAVAELRLFWREGFFEARDAFREAFAEFGTFERLSFLGKGEALVPGGYESWYNHY
                     TDISEAVIEGDLEAIDAEPNLIRDFYIERGKPTVFQIDDGWERAVGEWEVDEGKFPRG
                     LSRLAGRAEEKGLVPGLWLAPFLATKRSAVYRDHPDWILRGRGGKPVPAGWNPNWDGD
                     FYALDLSVPEVGDYLDAVFERVVEEWGFRYLKLDFLYAAFTPGVRRGGGAAYEHYDRT
                     VGRITSKIEDGRGRPVVWLGCGAPLEPSFRHFPLMRIGADTKEAWEWPILKAVRHEGR
                     PAAYANLLATFGRAVLDGSVFVNDPDVIFLRSRGMALSEAEKELVALADFLLASQIMF
                     SDNVSDFRDPAARAFSERIVALFDRLEEGEYGAERIARDVFSVFRRGGGVRGVANLSE
                     RPWSAPSGLHDRGRPILERASRSGGRTAFAARSISLYEE"
     gene            5695..7350
                     /locus_tag="PLG14_05920"
     CDS             5695..7350
                     /locus_tag="PLG14_05920"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOX48708.1"
                     /translation="MEKTVYVIGHKNPDTDSVVAAAAYAELKRALGMAEARAARAGAV
                     NPQTEYIFERFGVELPEFVPDLVPKVEYWLSGQADTVLDTTPLWEALAAMGEGDSKSL
                     PIVNSAGRYVSMLHYGAFARNILTKINPHKKAVIPTSVAHLVKTVKAQPLVTFDEGEF
                     FKARLVVAALETESFRRHILSEPAGNKVVLVGDREDVQRIAIEAGVRALIATSGTMVS
                     KELRELAERKRVSVLVSPYDTSSTSLLVIYSTPVGTMGDEGIAPVLRRDWIKTARKAL
                     AGSPSRSVPVLDDEGAVAGLFTEGDLIREPNVEVIMVDHNEFSQAVEGIENYRVLEVI
                     DHHRLGSFTTKYPITFINRVVGSTSTIVAGMYREQRVPLRKQVASVLLCGILSDTLVL
                     RSATTTEADRETAEYLANVSDLEIEALGKDIMGSASEVASRPAGEIIRLDLKEYESAG
                     RRLSVSQVEVSDTAEIMERRAEILSGLSSLRREGGYYLSAIMVTDITELTSLLLIDAD
                     KEFLSLVGYPKAEAGVFVLKDVLSRKKQLMPALFELIEKAVER"
     gene            7392..8072
                     /locus_tag="PLG14_05925"
     CDS             7392..8072
                     /locus_tag="PLG14_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOX48709.1"
                     /translation="MGRYPARGAEAPGSGRKGNMKRITSIVFDFGNVITLPQDKGPVA
                     AMAAALGGSTVEALEAAYAEGRGAYDRGDIGPAEYWAGVGERLGVRVDGALLSELKRL
                     DVESWFNMNGEMLAFIDSLRPRVRRLGLLSNINEEGVRQLRKDGGWLDCFDARILSCE
                     HRLLKPEPGIFELCLRELGEEASSCLFVDDLPENVEGARAVGMRGHVFKGLAGLKAEL
                     EDAYELVR"
     gene            8439..8990
                     /locus_tag="PLG14_05930"
     CDS             8439..8990
                     /locus_tag="PLG14_05930"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HOX48710.1"
                     /translation="MVLDLLVRRSNGGEGMEAVDLGAEATAAVVAAMERYAAEERNLS
                     GYDPGKGWAHSSAHAADVLASLAASPYVDRRGLERVLAAARTLLLKSESAYAHQECGR
                     IAKVLVAAYRRGDPARALVRPWIEEALEACPRAFADSAAYARWTNLLDLLRSLYFHLR
                     AEPDGAELADFVESKVRVMMRIA"
BASE COUNT         1347 a         3180 c         3177 g         1307 t
ORIGIN      
        1 ccccgccggc gcgacccgcc ccgacggcgt cgccgccgcc ggcgcggggt ccccggccgg
       61 cctggacttc gcggccctgg cccgcgcctg cctggagcgg acggaggccc tggtcgggcg
      121 ccacggcccc cgcctggccg gcggcgacgc ggggaaggcc gcggcccgcg agctcgcgga
      181 ggagctccgc ggcttctgcg acagcgtccg cgcggaggag ttcgaggccc atccggcctc
      241 cttctacgcc tacatgaaag tccttcccgc cgcctacctc ctgggtctcc tctcgatcct
      301 cgccttccgg ggcctctacc tcgcgcccct cctaggcctg gtgtcgggca tcgcgctcat
      361 ggcgtcgcag ttcggcctct acggccggct gggagaccgc ctctttccgc ggcggacctg
      421 cctgaacgtg gaagggatcg tggagccgac gggcgaggcc gagttcgagc tcatcgtctc
      481 cggccaccac gacagcgcgc cggtggcgag gatctactcg ggacccttcc agcgctggta
      541 cgccttcgcg atcttcgcgc cctatccttt cttcctactc caggcaggtc tcctcgcctc
      601 cttaatagca aggccgggct ccgctccgcc ggcctgggcg ccggccgtcc tcctttcggg
      661 cctgcccttc gcggtagcct acttcctcct cgtggacacg cgccgcggca gccccggcgc
      721 cggcgacaac ctcgtctcct cggtcctggc ggccacgatc gcgcgcgagc tcgccgcgcg
      781 gaagggcgag ctcctcaggc ggacccgact gcgcgcggcg agcttcgacg ctgaggaggc
      841 tggcctccgg ggctcggcgg cctacatgcg cgcccgccgc ggggagtcgg gcgggctccc
      901 cgtcctcatg ctcaacttcg actcgctcta cagcgcgtcc gagctccacg tcctcgtgag
      961 cgacgtgaac gggacggtca ggctctcccg cgggatggcc gaggacgcgg cggactgcct
     1021 ccgcgccgag ggccacgatc cgcggctcta ccccatggca ttcggaggcg gcgccaccga
     1081 cgcggccgag gccgcccggg ccggcgtcca ggcgacgacg gtcatcgcga tgccgacggc
     1141 cgtcgtgcgc gagggcctgg tctaccacac gccgcgggat agggtggagg ccatcgagcc
     1201 cggggtcgtg gaggcctgcc tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnncgcccg agtccggtcg atcaacggca agatcctctt caccttcctg ggcctctggc
     1381 tcctcggcgc ctccctcctc gtcttcatgt cctacgggta gacgagcaag gcgctcaagg
     1441 acagcatccg cgtccgggtc cgcgactacg ccgccctggg cgcgctgagc cttcccgccg
     1501 aggcccacgc gaggctcagg tcgccggagg acgaggcggg agaggactac gcggccgtcg
     1561 tcgcggcgct caggcggata gccgcccaca gcagcgacat cgtctacgcc tatacggtcc
     1621 gcaaacaagg cgacggcagc ctggtcttcg tcggggacgc gacggagccc gaggacgagc
     1681 cggcccgcct gggcgatccc gtcgaggagc ccacgcccct cctcagggcc tccgcgggag
     1741 gcatcgagga gaccgtcatg gaggaggagt tctacacgga caagtggggc accttcctga
     1801 gcgcctacgc gccgatccgg accgaggacg gtaccttcga cggcctcctt tgcctggaca
     1861 tctcttacgc ccaggtcagc aagatcatgc tgggccacct cctcgggctc ctcctcctcc
     1921 tcttcggctg ttcggccctg gtcgtgccgg tggccgtctt cagggcccgg tcgctgaccc
     1981 ggccgatcaa gcgcgtcagc caggccttcc gggagatatc ctccggcgag gtcaccgacc
     2041 tgacgaggcg cgtcgaggcc ctggactcgg acgagatcgg cgccatggcc gagatcgcga
     2101 acgggacctt cgagaagatg cggaacctcg tcacctccat ccgggcgcag gccggggcca
     2161 tctccgaggt cggcttcgag ctctccgcca acatgaccga gacggcggcc tccatcaacc
     2221 agatcagcgc gaacatccag agcgtcaagc ggctggcggg caaccaggcc cagagcgtcg
     2281 cggagacgaa ccacgcgatg gactagatca cgcagaacat caagcggctg gacggacaca
     2341 tcgagaagca gtccgacagc gtcggccgat cctcctccgc gatcgaggag atgctcgcga
     2401 acatcgcctc cgtgacgggc gtcctcaaca gcgacgcgcg gaacgtggcg gagctggcgg
     2461 cggcctcgga ggccggccgc gccgacctgg cctccgtctc cgagcgcatc cgtgaggtag
     2521 cgagggagtc cgaggggctc ctggagatca gctcggtgat ccagaacgtc gcctcccaga
     2581 ccgacctcct gtccatgaac gccgccatcg aggccgccca cgcgggggag tcggggaagg
     2641 gcttcgcggt cgtcgccgac gagatcagga agctcgccga gtcctccggc gagcagtcga
     2701 agaccatctc cgcctcgctg aagcggatca aggactccat ggaggtcatc accctctcca
     2761 cggacaaggt gatggaccag ttcgaggaca tcaactcgcg gatccgcgcc gtctccgagc
     2821 gggagaaggg catccgcgac gcgatggagg agcagggcgt cggcagcaag gagatcctgg
     2881 aggcggtcgg gaagctggcc gagatctcgg gcgaggtgcg gtcgggctcc gaggagatgc
     2941 tcgcgggcag ccgggaggtc atcaaggagt cggccagcct gggccggatc accgaggagg
     3001 tgacgggcag catggcggag atggccgcgg gggccgagca gatcaccgtc gccgtgaaca
     3061 aggtcaacga cctggccaag gacaacaagg agagcatcga cgccctgatg gccgaggtcg
     3121 cgaagttcaa ggtcgagtag gcgcgctggc cgctctctgg cggcccgccg cggcggacgg
     3181 gaagtcttcc gcgggcggcg ccgggcctag tccagggcct tcaccttctg gaagcgcttg
     3241 taggcctcct cgaagccctc gcggaggcag acctccagcg ccgaggcggc gcgggggaag
     3301 acggagcagt ccagggcctc gcgctcggcc ttcgtgaagt cggagaggac gtagccggcg
     3361 acgtcggggt ggtcggggcg gccgatgccg aagcgcaggc gggagaagtc gcgggtgccc
     3421 aggcgggcct ccagcgagcg caggccgttg tggcccccta ggccgccgcc ctgcttgaag
     3481 ccgaatacgc cgaagggcag ctcgagctcg tcgtggaccg cgaggatctc gtcgggcccg
     3541 atctggaaga agcgcatgag ctcggcggcc gagtcgcccg agaggttcat gtaggtctcc
     3601 ggcgcgagga gccagacgcg gccctggggg ccctcccact gggcgaagcg ccccttgaac
     3661 ttccgttcga agcggagctc cccgtagaag ggcaggctct ccaggaactg ccaggcgacg
     3721 ttgtggcggt tgccgcggta ctcgcggccg tggttaccga ggaaggcgta aaggcgtacc
     3781 atgatgctcc gggggcccgg tttgactgga ccgcccgccg atagtacact ggccgcgcga
     3841 ttttgtgtac cgacttcccc ggaggaccat gagcatgacc gcagatccgc gcctcgccct
     3901 ggacgtcgac gaggctgacg ggcaagtacg catccgcctc agggcggccg ccgacatcca
     3961 gttcagcgag gaggagcccg tcctcctccg cgccaccgtc ggggagctcc tgggcctggc
     4021 ccgggcgccc gaggccttcc gcgaggccct gctcgacccg cgccgccgcc tcctcgtcgc
     4081 gaacggctgg cagtcctggt cctacgccgg cgaactaggc ccgcgcgagc gggtgaaggc
     4141 ctgcctggta gggcgcttca ggcccttcag ccagcggccg gggaggctgg agcggcgcgg
     4201 cgagctcctc tcccacttct acgtcggcct ccgcgccggg gagggaaggc tcttcctcgt
     4261 gtcgcgcaac gccgggacgc cgcccctctc cttcaggctg gaccggagga gcctggacct
     4321 ctccgtggag gccctggccg gcggcgccgc cttcaaggcc ggccaggccg tcgccgagct
     4381 caggctcttc tggcgggagg gcttcttcga ggcccgcgac gccttccgcg aggccttcgc
     4441 ggaattcggg accttcgagc gcctgtcctt cctgggcaag ggcgaggccc tggtgcccgg
     4501 cggctacgag tcctggtaca accactacac ggacatctcc gaggccgtca tcgagggcga
     4561 cctggaggcc atcgacgccg agccgaacct gatccgcgac ttctacatcg agcgcggcaa
     4621 gccgacggtc ttccagatcg acgacggctg ggagcgggcg gtcggggagt gggaggtcga
     4681 cgaggggaag ttcccccgcg gcctttcccg cctcgcgggg cgcgccgagg agaagggcct
     4741 cgtgcccgga ctctggctcg cgcccttcct ggcgaccaag cgctccgccg tctaccgcga
     4801 ccacccggac tggatcctgc gcggcagggg cgggaagccc gtccccgcgg gctggaaccc
     4861 caactgggac ggggacttct acgccctgga cctctccgtc cccgaggtcg gggactacct
     4921 ggacgccgtc ttcgaacggg tcgtcgagga gtggggcttc cgctacctca agctggactt
     4981 cctgtacgcg gccttcacgc cgggtgtccg gcgcggcggc ggggcggcct acgagcacta
     5041 cgatcggaca gtcgggcgca taacgtccaa gatcgaggac gggcgcggga ggccggtcgt
     5101 ctggctgggc tgcggagcgc cgctggagcc gagcttcagg cacttccccc tcatgcggat
     5161 cggcgccgac acgaaggagg cctgggagtg gccgatcctg aaggccgtca ggcacgaggg
     5221 caggccggcc gcctacgcga acctcctcgc gaccttcggc cgggccgtcc tggacggctc
     5281 ggtcttcgtg aacgaccccg acgtcatctt cctgcgctcg cgcggcatgg cgctctccga
     5341 ggcggagaag gagctcgtcg cgctggccga cttcctcctg gcctcgcaga taatgttctc
     5401 cgacaacgtc tcggacttcc gcgaccccgc ggcgcgggcc ttctccgaga ggatcgtcgc
     5461 gctcttcgac cggctggagg agggggagta cggcgccgag cggatcgccc gggacgtctt
     5521 ctccgtgttc cggcgcggcg gcggggtccg gggcgtcgcc aacctgtccg agaggccctg
     5581 gtcggccccc tcgggcctcc acgaccgcgg gaggccgatc ctggagcgcg cctcgcgctc
     5641 gggcggccgg acggccttcg cggcccggtc catctcgctc tacgaggaat gaccatggaa
     5701 aagaccgtct acgtcatcgg gcacaagaac cccgacaccg actccgtcgt cgccgcggcg
     5761 gcctacgccg agctcaagcg ggccttgggg atggcggagg cgagggccgc gcgcgcagga
     5821 gccgtgaacc cccagaccga gtacatcttc gagcgcttcg gcgtggagct gccggagttc
     5881 gtgcccgacc tcgtgccgaa ggtggagtac tggctctccg gccaggcgga cacggtgctg
     5941 gacacgacgc cgctctggga ggccctggcc gcgatggggg agggcgactc gaagtccctg
     6001 cccatcgtga actccgccgg ccgctacgtg tccatgctcc actacggggc cttcgcgagg
     6061 aacatcctca cgaagatcaa cccgcacaag aaggcggtca tcccgacaag cgtcgcccat
     6121 ctcgtgaaga cggtgaaggc ccagcccctc gtcaccttcg acgagggcga gttcttcaag
     6181 gcccggctgg tcgtcgccgc gctggagacc gagtccttcc gccggcacat cctgtcggag
     6241 cccgcgggca acaaggtcgt cctggtcggg gaccgggagg acgtccagcg catcgcgatc
     6301 gaggccgggg tccgcgccct gatcgcgacg agcggcacga tggtctccaa ggagctcagg
     6361 gagctggccg agcggaagcg cgtctccgtg ctcgtctccc cctacgacac ctcgagcacc
     6421 tccctcctag tgatatactc gacgcccgtc ggcacgatgg gcgacgaggg gatcgcgccg
     6481 gtgctgcgcc gcgactggat caagaccgcc aggaaggccc tggccggaag cccgagccgc
     6541 tccgtgcccg tcctggacga cgagggcgcg gtggcggggc tcttcaccga gggcgacctg
     6601 atccgcgagc cgaacgtcga ggtgatcatg gtcgaccaca acgagttctc ccaggccgtg
     6661 gagggcatcg agaactaccg cgtgctggag gtgatcgacc accaccgcct gggctccttc
     6721 acgaccaagt atcccatcac cttcatcaac cgcgtggtcg gctccacgag cacgatcgtc
     6781 gcgggcatgt accgcgagca gcgcgtgccc ctgcgcaagc aggtggcctc cgtcctcctc
     6841 tgcggcatcc tctccgacac cctggtcctg cgctccgcga cgacgacgga ggccgaccgg
     6901 gagacggccg agtacctggc gaacgtctcc gacctggaga tcgaggccct gggcaaggac
     6961 atcatgggct ccgcctccga ggtcgcctcc aggcccgccg gggagatcat caggctggac
     7021 ctgaaggagt acgagagcgc cgggaggcgg ctctcggtga gccaggtcga ggtctccgac
     7081 acggcggaga tcatggagcg ccgcgccgag atcctctcgg ggctctcctc gctcaggcgc
     7141 gaggggggct actacctctc cgcgatcatg gtgaccgaca tcacggagct cacgagcctc
     7201 ctcctcatcg acgccgacaa ggagttcctc tccctggtcg gctaccccaa ggcggaggcg
     7261 ggggtcttcg tcctcaagga cgtcctctcc cggaagaagc agctcatgcc ggccctcttc
     7321 gagctcatcg agaaggccgt ggagagatag gcagccccgc gcggcgaggg cctccgggac
     7381 ggcgcgggcc tctggggcgg taccccgccc gaggcgccga ggcccccggg agcgggcgga
     7441 aagggaatat gaagcgcata acgtcaatag tcttcgactt cggcaacgtg atcacgctgc
     7501 cccaggacaa gggcccggtc gcggccatgg cggccgccct gggcggctcc acggtggagg
     7561 ccctggaggc cgcctacgcg gagggccgcg gcgcctacga ccggggcgac atcggcccgg
     7621 cggagtactg ggcgggggtc ggggagcggc tgggcgtccg cgtggacgga gccctccttt
     7681 ccgagctcaa gcggctggac gtggaaagct ggttcaacat gaacggcgag atgctcgcct
     7741 tcatcgattc cttgaggccc cgggtgcggc gcctgggcct cctctccaac atcaacgagg
     7801 agggcgtgag gcagctgcgg aaggacgggg gctggctcga ctgcttcgac gcgaggatcc
     7861 tctcctgcga gcatcgtctt ctcaagcccg agcccggcat cttcgagctc tgcctccgcg
     7921 agctcgggga ggaggcctcc tcctgcctct tcgtcgacga ccttcctgag aacgtcgagg
     7981 gcgccagggc cgtcgggatg cggggccatg tgttcaaggg gcttgccggc ctcaaggccg
     8041 agctcgagga tgcctatgag ctcgtcaggt agcgcgcgcc gcccgggcag cgcggccgcc
     8101 gccgaagccg ctcgcggaat gccgacgctg gccgccgagg ccgcccgcga ggcggccgcc
     8161 aaggccgagc tcaagtccct ggaggcgaag ggcttccccg cccccgaagg cgagagggct
     8221 aatcctatct cgaccggaac ctggaactgc tgggctccct ggaccccgag ctccgcgacg
     8281 gcctcttcta cgaggcggcc ggcgcctggc tggagcgctg ccccttctcc cgggaggagc
     8341 tccaagccct ggccgagagg ctcgtctccg gcgacttcct cttccggggc atagaagatc
     8401 cttcggggga cgaggtctac aggcggacct tctccgccct ggtcctggac cttctggtca
     8461 ggcgctccaa cgggggcgag ggcatggagg ccgtggacct cggggccgag gcgacggcgg
     8521 ccgtcgtcgc cgcaatggag cgttatgcgg ccgaggagcg gaacctttcg ggctacgatc
     8581 ccgggaaggg ctgggcccac tcaagcgcgc acgcggccga cgtcctcgcc tcgctcgcgg
     8641 cctcgccgta tgttgaccgg cggggcctcg agcgcgtcct cgcggccgcg cggaccctcc
     8701 tgctcaagag cgaaagcgcc tacgcgcacc aggagtgcgg gcggatcgcg aaggtcctcg
     8761 tcgcggcata caggcggggc gatcccgccc gcgccctggt ccggccctgg atcgaggagg
     8821 ccctggaggc ctgtccgcgc gccttcgcgg acagcgcggc ctatgcgcgg tggacgaacc
     8881 tcctcgacct gctgcgctcc ctctacttcc acctcagggc cgagccggac ggcgcggagc
     8941 tcgcggactt cgtggagtcc aaggtccgcg tcatgatgag gatcgcctag gccgcgcggt
     9001 cggccgccgg cgagccccgg gcgggcgcgc ggcggccggc gctttggacc gcggccgccg
     9061 tcaggaggcc gagccgcagc acttcttgta tttcttcccg gagccgcagg g
//
LOCUS       DAOOSJ010000091         9055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4884_length_9055_cov_6.267889, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000091 DAOOSJ010000000
VERSION     DAOOSJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_9055_cov_6.267889"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLG14_05935"
     CDS             <1..769
                     /locus_tag="PLG14_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOX48711.1"
                     /translation="EALRELSGRLEEMPAEEGFPAYLPTRLAEFYERGGRVETLGGKV
                     GSVSIIGAVSPPGGDFSEPVTQHTKRFIRCFWALDRDLANARHYPAVSWTDSYSEYAE
                     DLGAWWDRVDPDWRGVRTRALELLKREQKLQDIVRLIGPDALPDEQRLVLITAEMLKS
                     GFLQQSSFDEVDRYCAPEKEVLLLRCLMEFHRRAAGAIKAGAPLPKVVALPVREKLVR
                     LKSSVPNDRMEDIRAAEGELSAAFDDLERQYRKEALR"
     gene            766..>2233
                     /locus_tag="PLG14_05940"
     CDS             766..>2233
                     /locus_tag="PLG14_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOX48712.1"
                     /translation="MRGVEHRGLSAAEGPIVMAERRESAGYGDLVAVYGRDGGRRLGR
                     VVEVGDKAMAIQLFSDNAGLSLDEARVECLGKPLELRVGEGLLGRVFDGLGRPADGYP
                     EILSSEKRDVNGRPLNPSARIYPRDFIQTGVSAIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIVRQAKILKKAAGAEGSVDKGQAGAAGQDGGVHQAGAGEGGAGSEAFAVVFSGM
                     GIKHDVARFFIDSFERSGVLSRVVCFLSLADAPSIERLVTPRSALTAAEYLAYELGMH
                     VLVVMTDMTNYCEALREVSAARGEVPSRKGYPGYLYSDLASLYERAGRIEGSPGSITQ
                     IPILSMPNDDISHPIPDLTGYITEGQIVLERELFQKGVYPPVAGLPSLSRLMKDGIGE
                     GMTREDHRDLASQLFAAYARVRSVRNLAAIIGEEELSPLDKKYLRFGERFEAEFLAQG
                     EFEDRSIAQTLDLGWRLLRHLPREELLRLPPELIAKYLP"
     assembly_gap    2234..2333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2348..2980
                     /locus_tag="PLG14_05945"
     CDS             2348..2980
                     /locus_tag="PLG14_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOX48713.1"
                     /translation="MAVQNVAPTKSNLLREKERLALALEGYDLLDRKREILVMELMRR
                     VDEVKMLEREIEARCGSAYPAMRRMLLSVGRERALGLSAGMKCDFAARERRVQVAGLS
                     VPSLEAEAPARELPYSFVNSFADSDQTMLEFTELLKLVAQMAGLRSVVWRLAKELKKT
                     QRRVNALEKMVIPESRGRKTYIEAALEERDRESVFAGKLLKARRAGKGDL"
     gene            2977..3459
                     /locus_tag="PLG14_05950"
     CDS             2977..3459
                     /locus_tag="PLG14_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX48714.1"
                     /translation="MKGPLAELLVVVNGAESSIEAAKFAMAMARAYASRVTAVYVVDT
                     ATIRQLALSRIFVPDEGEEYERSLEETGRRYLSYVEELAKEKRFHVQTRLLKGAIAGE
                     VVKAAAETGADCVVIGGGDSGSAYRDAILESYREILRNSPCPVLVVRGRKADEAYRAL
                     "
     gene            3488..3901
                     /locus_tag="PLG14_05955"
     CDS             3488..3901
                     /locus_tag="PLG14_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48715.1"
                     /translation="MRVAVVYVPSGRAEALAGLSASLAAAFEAAGHRAEILEPGQAIA
                     RLAAFDYVAFGTEGRGFGGKIPAKVAETLAQARGLSGKRSLAFVRKSAFGSARALSRL
                     MAAMEAEGMYVNDSAIVGTAEEAAAAAKSAPVERN"
     gene            3905..>4494
                     /locus_tag="PLG14_05960"
     CDS             3905..>4494
                     /locus_tag="PLG14_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOX48716.1"
                     /translation="MDGYYDILGVPPEASAKEVKSAFRRQAKKLHPDMAGQAKVEEGV
                     RSREAAMRRLLEAYRMLSDPERRRAYDRSLRRRAAEEGGFDYRKFLKSRPDDPSSQAK
                     LVVYDLLHGLEDEALEIYERSKSLGDFRLERFLERGEAMDAEFCVAEEYEKRERWLKA
                     YGLYKRLIVMELERPWFRYFFDVVALRFRTLLLLKLP"
     assembly_gap    4495..4594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4712..5740
                     /locus_tag="PLG14_05965"
     CDS             4712..5740
                     /locus_tag="PLG14_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX48717.1"
                     /translation="MKRSLAAAAFSLVCAAAFAQTAIDKPAATLKLTKQEVISVRQLK
                     ADVERIEAAIGQKLPLEKRKELLDSKINGMLFVQYCEREKILAPEAEVNAALAQMKAG
                     LGKGADDAKLAEALRAQGVLLDAKSYARQQILLRSYLQQKRAADLKAVKEPSSDEVLK
                     AYELYKSKLVRPDTVRASVLFVDLRNLSADDKKKAVDAMRVAASQVKGSSQKFDELVL
                     KAGDQGSVVKATPTIYVEKTPEFQNVYGAKFMDAAFRLKAGEMSDLIENEAGLQIVRV
                     NEVLPQKQLALSDPLPGNPQATVQDYLKYNLAMERQNEVLSRIQAELVAQLRKEAVVK
                     IFEETLSF"
     gene            5751..6173
                     /gene="nusB"
                     /locus_tag="PLG14_05970"
     CDS             5751..6173
                     /gene="nusB"
                     /locus_tag="PLG14_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOX48718.1"
                     /translation="MASRRKARILAFQALYAWDASKSALRELLDFSWLDQEKRDGLEE
                     ELKAFARILIAGTIENIEAIDVSIKSHLQNWSFERLKRVDLAILRIGAYSLLFQGDIP
                     AQITIDEAIEIAKEYGSDDSYRFINGVLDGIWKSAGAR"
     gene            6142..7947
                     /locus_tag="PLG14_05975"
     CDS             6142..7947
                     /locus_tag="PLG14_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48719.1"
                     /translation="MESGSRPAPGRGRRPRRGAGRRCAAAASRALLASSLLLLFACSR
                     GPRDFAEASAALDEALAGAPSERELDRAFEAAYRKAKGSTEWLSILKRAARADALAVA
                     SGSTAAARGADSAERALKAMPGSEPVAAAAAYLFLRGGVPEKALALFKEALKPEDRPE
                     LWAEAFLSSLRAGSRPSPADAPAAYDRLARVLGDPRFFTDSAAASLAAGDGFAARATL
                     GLAALRGAAPPDELLWDAGLPALLAARSDAGAGPERLRLLADAAWLSGDAALAKARWE
                     RAISLAPKASYKPYASLLAAEAAEAAKAAALASLDDASREGRGPAKAYDDSVAPPFSF
                     DPSAPPPRAAAGGAGARSPEGEGGFPRSPGRASSADYERLMRLFPDESAPKALYAAAL
                     AREGRGAEALGLLLSGGDEAADSLSARVALAAAAGRWPEDRLAGQAFRLAETRPEDGV
                     LLDSVLRLLLARGRNADFLVLYRAAESRGLSYPRGAFHAAAAAALTGDYRAAVAVLEK
                     DGPSEPGPEAAFALGLLYSRLGDQALAADRLAVALSAARSGAERCRALKATGDARAAA
                     GDPEGALAAYRAAAAADPGDAEAASLSRGKRPARP"
     gene            8005..8766
                     /gene="map"
                     /locus_tag="PLG14_05980"
     CDS             8005..8766
                     /gene="map"
                     /locus_tag="PLG14_05980"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOX48720.1"
                     /translation="MIRLKNAAQIDRIRESCRMLSGLFAEIAPLVRPGVSTRELDDAA
                     REYILSRGGKPAFLGYDGYPASLCVSPNEVVIHGIPDGRRLAEGDIVGLDCGIDLGGY
                     FSDAAITLPVGRISEEAEKLLRVTRECLERAIAAVKPGARIHDVSRAVYGHAVSNGYG
                     VVRQYCGHGVGLSPHEDPQVPNYVSPGPNPRIVPGMVLALEPMVNLGTGEIKVLGDDW
                     TVVTLDKRLSAHYEHTVAVLPDGTAEVLTSWTYPG"
     gene            8866..>9055
                     /locus_tag="PLG14_05985"
     CDS             8866..>9055
                     /locus_tag="PLG14_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOX48721.1"
                     /translation="MSLLKSLYSRKFFLFNLVLVGVLVGFALAFVGFSAGARGPDRFV
                     RAETRGGAPVVLVDAPAIA"
BASE COUNT         1339 a         3302 c         2934 g         1280 t
ORIGIN      
        1 cgaggccctc cgcgagctct cggggcgcct ggaggagatg ccggcggagg agggcttccc
       61 cgcctacctc ccgacccggc tcgcggaatt ctacgagcgc ggcggccgcg tggagaccct
      121 tggcgggaag gtcggctcgg tgtccatcat cggcgcagtc tcccctccgg gaggcgactt
      181 ctccgagccg gtcacccagc acacgaagcg cttcatccgc tgcttctggg ccctggaccg
      241 cgacctcgcc aacgcgaggc actatccggc cgtctcctgg acggactcct actcggagta
      301 cgccgaggac ctgggcgcct ggtgggacag ggtggacccc gactggcggg gcgtcaggac
      361 gagggctctc gagctcctga agcgcgagca gaagctgcag gacatcgtgc gcctcatcgg
      421 ccccgacgcc ctgccggacg agcagcgcct ggtcctcatc acggcggaga tgctcaagtc
      481 gggcttcctc cagcagagct ccttcgacga ggtggaccgc tactgcgcgc cggagaagga
      541 ggtcctcctc ctccgctgcc tcatggagtt ccaccgccgg gcggctggcg cgatcaaggc
      601 cggggcgccc ctgcccaagg tggtcgcgct tcccgtccgc gagaagctcg tcaggctcaa
      661 gagctccgtc cccaacgacc ggatggagga catccgagcg gcggagggcg agctgtcggc
      721 cgccttcgac gacctggagc ggcagtaccg caaggaggcc ctgcgatgag gggcgtcgaa
      781 caccggggcc tctcggccgc cgagggaccc atcgtgatgg cggagcgccg cgagagcgcg
      841 ggctacgggg acctggtcgc ggtctacggc cgcgacgggg ggaggcgcct cgggcgcgtc
      901 gtcgaggtgg gcgacaaggc catggcgatc cagctcttct ccgacaacgc gggcctctcc
      961 ctcgacgagg cccgcgtcga gtgcctcggg aagcccctgg agctcagggt gggcgagggc
     1021 ctcctgggcc gcgtcttcga cggcctcggc cggccggccg acggctatcc cgagatcctc
     1081 tcctccgaga agcgggacgt gaacggccgc cccctgaacc cctcggcccg aatctacccg
     1141 cgggacttca tccagaccgg cgtctccgcc atcgacggca tgaacaccct gatccgcggc
     1201 cagaagctcc cgatcttctc gggcaacggc cttccgcaca accgcctggc cgcccagatc
     1261 gtgcgccagg cgaaaattct caagaaggcc gccggggccg aaggctccgt cgacaagggc
     1321 caggccggcg ccgccggcca ggacggcggc gtccaccaag ccggcgcagg ggagggcggc
     1381 gcgggctcgg aggccttcgc cgtcgtcttc tcgggcatgg gcatcaagca cgacgtggcg
     1441 cgcttcttca tcgactcctt cgagcgctcg ggcgtcctct cccgcgtcgt ctgcttcctc
     1501 tccctcgcgg acgcgccctc catagagcgc ctcgtcacgc cgcgctccgc cctcaccgcc
     1561 gcggagtacc tggcctacga gctcgggatg cacgtcctcg tcgtgatgac cgacatgacc
     1621 aactactgcg aggccctccg cgaggtctcc gcggcgcggg gcgaggtgcc ctcccggaag
     1681 ggctacccgg gctacctcta ctcggacctc gcctcgctct acgagagggc gggccgcatc
     1741 gagggctcgc cgggctcgat cacgcagatc cccatcctct cgatgccgaa cgacgacatc
     1801 tcgcacccga tccccgacct cacgggctac atcaccgagg gccagatagt cctggagcgg
     1861 gagctcttcc agaagggcgt ctacccgccc gtcgccggcc tgccctccct gtcccgcctc
     1921 atgaaggacg gcatagggga ggggatgacg cgcgaggacc accgcgacct cgcgagccag
     1981 ctcttcgccg cctacgcccg cgtgcggagc gtgcggaacc tggccgccat catcggcgag
     2041 gaggagctct cccccctcga caagaaatac ctccgcttcg gcgagcgctt cgaggcggag
     2101 ttcctggccc agggcgagtt cgaggacagg agcatagccc agaccctcga cctgggctgg
     2161 cgcctcctcc gccacctccc ccgggaggag ctcctcaggc ttccccccga gttgatcgcg
     2221 aagtacctgc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggggagg
     2341 cctagccgtg gccgtccaga acgtcgcgcc caccaagtcg aacctcctcc gcgagaagga
     2401 gaggctcgcc ctggccctgg agggctacga cctcctcgac aggaagcggg agatcctcgt
     2461 catggagctc atgaggcgcg tggacgaggt gaagatgctc gagcgcgaga tcgaggcccg
     2521 ctgcggctcg gcctatcccg cgatgcggcg catgctcctc tccgtgggcc gcgagcgggc
     2581 cttgggcctc tccgccggga tgaagtgcga cttcgccgcc agggagcggc gggtccaggt
     2641 ggcggggctc tccgtcccga gcctggaggc cgaggccccg gcccgggagc tgccctattc
     2701 cttcgtgaat tcgtttgccg attcggatca aaccatgcta gagttcaccg aactcctcaa
     2761 gctcgtcgcc cagatggcgg gcttgagaag tgtcgtctgg cgcctcgcga aggagctcaa
     2821 gaagacgcag aggcgggtga acgccctcga gaagatggtc atccccgagt cccggggccg
     2881 caagacctac atcgaggcgg cgctcgagga gcgcgatcgg gagtccgtgt tcgcgggcaa
     2941 gctcctcaag gcccgtaggg cggggaaggg cgatctatga agggccccct ggccgagctt
     3001 ctcgtcgtcg tgaacggcgc cgaatcctcg atagaggccg cgaaattcgc catggccatg
     3061 gccagggcct acgcctcgcg ggtcaccgcg gtgtacgtgg tcgacacggc cacgatacgc
     3121 cagctcgcgc tgtcgcggat cttcgtcccg gacgagggcg aggagtacga gcgcagcctc
     3181 gaggagacgg ggcggcgcta tctctcctac gtcgaggagc tcgccaagga gaagcgcttc
     3241 cacgtccaga ccaggctcct caagggggcc atcgcgggcg aggtggtcaa ggccgccgct
     3301 gagaccggcg ccgactgcgt cgtgatcggc ggaggggact cgggctcggc ctaccgggac
     3361 gcgatcctcg agtcctaccg cgagatcctc aggaattccc cctgtcccgt cctcgtcgtc
     3421 agggggcgga aggccgacga ggcctatcgc gccctgtaag ggccgcaaca tcgctttcgg
     3481 aggccgcatg cgcgtcgccg tcgtctacgt gccgtcgggg agggccgaag ccctcgccgg
     3541 cctttccgct tccctggccg ccgccttcga ggccgcgggc cacagggcgg agatcctgga
     3601 gccgggccag gccatcgcga ggctcgccgc cttcgactac gtcgccttcg gcaccgaggg
     3661 gcggggcttc ggcggcaaga tccccgcgaa ggtggccgag accctcgccc aggcccgcgg
     3721 cctctcgggc aagcggagcc tggccttcgt gcggaagtcg gccttcggct ccgccagggc
     3781 cctctcccgc ctcatggcgg cgatggaggc cgagggcatg tacgtgaacg actcggccat
     3841 cgtcggcacc gcggaagagg ccgcggccgc cgcgaagtcc gcgccggtgg aacggaacta
     3901 ggccttggac ggatactacg acatcctcgg cgtccccccg gaggcgagcg ccaaagaggt
     3961 gaagagcgcc ttccggcgcc aggccaagaa gctacatccc gacatggccg gccaggccaa
     4021 ggtcgaggag ggggtccgct ccagggaagc cgcgatgcgc cgcctcctcg aggcctaccg
     4081 catgctctcc gatcccgaga ggcgacgcgc ctacgaccgc tccctgcgcc gccgcgcggc
     4141 ggaggagggc ggcttcgact accgcaagtt cctcaagtcc aggcccgacg acccctccag
     4201 ccaggcgaag ctcgtcgtct acgacctcct ccacggcctg gaggacgagg ccctggagat
     4261 ctatgagcgc tccaagtccc tgggcgactt ccggctcgag cgcttcctgg agcgcggcga
     4321 ggcgatggac gcggagttct gcgtcgccga ggaatacgag aagcgcgagc gctggctcaa
     4381 ggcctacggc ctctacaagc gcctgatcgt catggagctg gagcggccct ggttccgcta
     4441 cttcttcgac gtggtcgccc tgcgcttcag gaccctcctc ctcctcaagc tcccnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccgc ggcttgcgaa aagatccttt
     4621 ttctattacc ctggcccggt tgatttgttt caataaaggc ggcccacgaa ggggcgcccc
     4681 tcgataccac ggttccttca ggaaggttca catgaagcgc tccctagccg cagcggcgtt
     4741 ctccctcgtt tgcgccgccg ccttcgcgca gaccgcgata gacaagcccg ccgccaccct
     4801 caagctcacg aagcaggaag tcatctccgt caggcagctc aaggccgacg tcgagaggat
     4861 cgaggcggcc atcggccaga agctgcccct cgagaagcgc aaggagctcc tcgactcgaa
     4921 gatcaacggc atgctcttcg tccagtactg cgagcgcgag aagatcctcg cccccgaggc
     4981 cgaggtgaac gccgcgctgg cgcagatgaa ggccggcctg ggcaagggag ccgacgacgc
     5041 gaagctcgcc gaagccctca gggcccaggg cgtcctcctg gacgccaaga gctacgctag
     5101 gcagcagatc ctcctccgga gctacctaca gcagaagcgc gccgccgacc tcaaggccgt
     5161 gaaggagccg agctcggacg aggtgctcaa ggcctacgag ctctacaagt cgaagctcgt
     5221 ccgccccgac accgtgaggg cctccgtcct cttcgtcgac ctcaggaacc tgtccgcgga
     5281 cgacaagaag aaggcggtcg acgcgatgag agtggccgcc tcccaggtca agggctcatc
     5341 ccagaagttc gacgagctcg tcctcaaggc cggcgaccag ggctccgtcg tcaaggcgac
     5401 cccgaccatc tacgtcgaga agacccccga gttccagaac gtctacggcg ccaagttcat
     5461 ggacgcggcc ttcaggctca aggccggcga gatgagcgac ctcatcgaga acgaggcggg
     5521 cctccagatc gtccgcgtga acgaggtcct gccccagaag cagctcgccc tctccgaccc
     5581 cctgcccggc aacccccagg ctacggtcca ggactacctc aagtacaacc tcgcgatgga
     5641 gcgccagaac gaggtcctct cgaggatcca ggccgagctc gtcgcccagc tccgcaagga
     5701 agccgtcgtg aagatcttcg aagagaccct ctccttctag gggccggcgc gtggcatcca
     5761 gaagaaaggc gcgcatcctg gccttccagg ccctctacgc ctgggacgcc tccaagtcgg
     5821 ccctccggga gctcctcgac ttctcctggc tcgaccagga gaagcgggac ggcctggagg
     5881 aggagctaaa ggccttcgcc cgcatcctca tcgcgggcac aatagagaac atcgaggcca
     5941 tcgacgtctc catcaagtcg cacctccaga actggtcctt cgagcggctc aagcgcgtcg
     6001 acctcgcgat cttgcggatc ggcgcctact cgctcctttt tcagggcgac atcccggccc
     6061 agatcacgat cgacgaggcc atcgagatcg ccaaggaata cggctccgac gattcctacc
     6121 gcttcatcaa cggagtgctc gatggaatct ggaagtcggc cggcgccagg tagaggccgc
     6181 cggccccgcc ggggcgcggg gaggcgctgc gcggccgcgg cctcccgcgc cctcctcgcg
     6241 tcgtcccttc ttctcctctt cgcctgctcc cgaggccccc gcgacttcgc ggaggcgagt
     6301 gcggccctgg acgaggccct ggccggcgct ccctcggagc gcgagctgga cagggccttc
     6361 gaggcggcct accgcaaggc gaagggctcc acggaatggc tctccatcct caagcgcgcc
     6421 gccagggccg acgccctcgc ggtcgcgtcc ggctcgacgg cggccgctcg cggcgccgac
     6481 tcggcggagc gggccctcaa ggccatgccc ggctccgagc ccgtcgccgc cgcggcggcc
     6541 tacctcttcc tgcgcggcgg cgttccggag aaggccctgg ccctcttcaa ggaagccctg
     6601 aaacccgaag accgtcccga gctctgggcc gaggccttcc tatcctcgct ccgcgcgggc
     6661 tcgcggcctt cgccggccga cgcacccgcg gcctacgacc gccttgcccg cgtccttggc
     6721 gacccccgct tcttcactga ctccgcggcc gcctccctcg cggccggcga cggcttcgcc
     6781 gcccgcgcca ccctgggcct ggccgccctg cgcggggccg ctccgcccga cgagctcctt
     6841 tgggacgcgg gcctccccgc cctcctcgcg gcgcggtccg acgcgggcgc cggccccgag
     6901 aggctccgcc tcctcgcgga cgcggcctgg ctctccggcg acgccgccct cgccaaggcc
     6961 cgctgggagc gggccatctc cctcgcgccg aaggcttcct acaagcccta cgcctccctc
     7021 ctcgcggccg aggccgcgga ggccgccaag gcggcggccc tcgcctctct ggacgacgcg
     7081 tcgcgggagg gccgaggacc cgccaaggcc tacgacgatt ccgtcgctcc gcctttttcc
     7141 ttcgatcctt ccgcgccgcc tccccgcgcc gcggcgggcg gggccggcgc gcgctcgccg
     7201 gagggggagg gcggcttccc ccggagcccc ggccgagcct cctccgcgga ctacgagcgc
     7261 ctcatgcgtc ttttccccga cgagagcgcc cccaaggccc tgtacgcggc cgccctcgcg
     7321 agggagggcc gcggagccga ggccctcggc ctcctcctct ccggaggaga cgaggccgcc
     7381 gactcgctct ccgcccgcgt cgccctggcc gcggccgcgg gccgctggcc cgaggacagg
     7441 ctcgccggcc aggccttccg cctggccgag accaggcccg aggacggagt gctcctcgac
     7501 tcggtcctga ggcttctcct cgcccgcggc aggaacgcgg acttcctcgt cctgtaccgc
     7561 gcggcagaga gccgcggcct ttcgtatccg cgcggcgcct tccacgccgc cgcggccgcc
     7621 gccctcacgg gcgactatcg cgcggccgtc gccgtcctgg agaaggacgg gccctccgag
     7681 cccggccccg aggcggcctt cgcccttggc ctcctgtatt cgcgcctggg agaccaggcc
     7741 ctcgcggccg accgcctggc cgtggccctg tcggccgccc gctccggagc cgagcgctgc
     7801 agggcgctca aggcgacggg cgacgcgcgg gccgccgcgg gggatcccga gggcgccctc
     7861 gcggcctacc gggccgccgc ggccgccgat cccggggacg cggaggccgc gagcctctcc
     7921 cgcgggaagc ggcccgcccg accctagcct ggaaacgaac cggcgccgcg acggcgccag
     7981 cgccttcggg cgcctggagg tacgatgata cgcttgaaga acgcggccca gatcgaccgc
     8041 atccgcgagt cctgccgcat gctttccggc ctcttcgccg agatcgcgcc cctcgtgagg
     8101 cccggtgtct ccacccgcga gctcgacgac gcggccaggg agtacatact ctcccgcggg
     8161 ggcaagcccg ccttcctggg ctacgacggc tatcccgcga gcctctgcgt ctccccgaac
     8221 gaggtggtca tccacggcat ccccgacggc cgccgcctgg cggagggcga catcgtgggc
     8281 ctggactgcg gcatagacct gggcggctac ttctccgacg cggccatcac cctccccgtc
     8341 ggcaggatct cggaggaggc ggagaagctc ctccgcgtga cgcgcgagtg cctggagcgg
     8401 gcgatagccg ccgtcaagcc gggagcgagg atccacgacg tctccagggc cgtctacggc
     8461 cacgccgtgt cgaacggcta cggcgtcgtc aggcagtact gcggccacgg cgtgggcctt
     8521 tccccccacg aggacccgca ggtcccgaac tacgtgagtc ccggcccgaa cccccgcatc
     8581 gtcccgggca tggtcctcgc gctcgagccg atggtcaacc tcggcacggg cgagatcaag
     8641 gtcctgggcg acgactggac ggtcgtgacc ctcgacaaga ggctctccgc ccactacgag
     8701 cacaccgtcg ccgtgctccc cgacgggacg gcggaggtcc tcacctcctg gacctacccc
     8761 ggctgagccc cgggccattt ttctcgagcg aaggcggccc ggagtaacct cttcgggccg
     8821 ctcggcgtta tattggtaca tacagcgttt cgcaaggagc acggcatgtc gcttctcaag
     8881 agcctttact ccaggaagtt cttcctcttc aacctcgtcc tcgtgggcgt cctcgtgggc
     8941 ttcgccctcg ccttcgtggg cttctccgcg ggcgcgcgcg ggcccgaccg cttcgtccgg
     9001 gccgagacga ggggcggtgc tccagtcgtc ctcgtcgacg cgccggccat cgcca
//
LOCUS       DAOOSJ010000092         9055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4885_length_9055_cov_6.018667, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000092 DAOOSJ010000000
VERSION     DAOOSJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4885_length_9055_cov_6.018667"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1926)
                     /locus_tag="PLG14_05990"
     CDS             complement(100..1926)
                     /locus_tag="PLG14_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48722.1"
                     /translation="MTRSPRPRAAAIVALILLALLSSSCIEGSPAGPALPLVPKEAVL
                     AVVVEKPAKLYASLEGFWKSAGVAALAGGELGELLQKSLPNEADIEKALDAARPWVLA
                     FVPAAPGSKEMRSLVYLPYRGQYFEALAPLASGKFQVLAQSKGYVVYSDGEYPLVFPP
                     TETLDLSVLARYPADSVKLWANPVALQRMGGKDFAGFQADVRRFVEEGAAPSPQEALK
                     ALEEGALALLKELGSADAALSFGAEGLTVRAAANVIPGASMEKFLSRAAQAPSALDWA
                     GQVSADALYGYAWSIDPGVAAELSDLYLRVALPALSGSMGPESAQLKAFFEKSAALQA
                     KWLGLQGPRGAASLDMDFDFAALTALGQSSGDDPKALADGIKKAMTIRLDMFQELKDE
                     AKYRALLSGVAKDPEVAALLKGFGESYGIGMEIRQADRKEGSFSYGEMGFSFKVLDEE
                     KFAASEGEGGAEAARAALEAVSGLFNLRYATSKGRFVATLGSPAELKALVERSQAPRS
                     LAADPAFAAFAKRLPAKVQVVGALSTRRLAAMIAKAVPADSGVVLDPSRFGSWYSYLA
                     AEGKAGASRLEFGLMIPAGDFSSIFNLVNAVKRIGAGAGQDL"
     gene            2156..4183
                     /locus_tag="PLG14_05995"
     CDS             2156..4183
                     /locus_tag="PLG14_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX48723.1"
                     /translation="MKELGKALLSEEPFSEIVIGDTAVARALVEARVRVISSYPGSPT
                     PEIAEALAAIPKGERPFYFEFSVNEKVALELAFGAAINGHASAVFFKSVGLNVAADSF
                     VQLSLMDIPGGLVVILGDDPGANSSQNEQDNRHYARLAYLPLLEPATAQEAYEMTLEA
                     SRLARERRTAVILRLTTHVCHAKEKVAFGAYRPEPLDRTPRFDPAASNYIPIAATVFP
                     LKRRALERLEAFRGDFEASPLNRILPGGDPRRGVIAAGTARLSLLDALEELPPERRPA
                     VLELATVHPLPRRLVARFLAGLAEAKILEELDPLLEEEIKALAYDEGLATKILGKADP
                     EELLGGYLPAKVRAILAAAWPDLGLAPPASALAARAAEAAAGAATGTAAAAARAPRGR
                     ESVPARPPQLCPGCGHRSAFHAVRKALREGDITVADIGCHTLGHLPPYKMGTVLLSMG
                     HSTSTASGLSLFNDSRRVLCFIGDSTFFHAGMPGIVNAVYNSHNVTLVVMENGTTAMT
                     GHQDHPGAGRNWSGEAPKIGIREALAGLGVAKVREVDAYRQAELEAAVREALAEPGFK
                     VVIARHPCMLKLGREARRKPGYVPRRVAVDQGACARIHACVSVFACPSFQLGRDGSVA
                     VSRELCVGDGSCRQVCPAGAILPPAVEAPGPGGRGDEAGEAARGGAKGGKP"
     gene            4180..4749
                     /locus_tag="PLG14_06000"
     CDS             4180..4749
                     /locus_tag="PLG14_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOX48724.1"
                     /translation="MTERYDIYMAGVGGQGIGLLSEALARAADKAGLPVRGCDTHGLA
                     QRGGVVTSHLRLGRAAHSPLVSPGGADLVVALERHEALRAARDYLKEGGVLVWYDAVF
                     QPLEVRLGKAAEVGAAEVEEECGRKGARAVLVLREDLPDPRMQNVALLSALAELGLPP
                     GLRREHLKAALEDLMEGAALERNLSVLEP"
     gene            complement(4877..5599)
                     /locus_tag="PLG14_06005"
     CDS             complement(4877..5599)
                     /locus_tag="PLG14_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX48725.1"
                     /translation="MMKTYLKSTGLLLGGVFLLGYILNVAQAIIAREPLLPLLTGWMM
                     VTQFAMALIFFASAFVHQMQWAQPLTFILMTPLGMIESRESFYGLGAFVIGVLFLFRM
                     GFYEKRRVLRLVLSLAYLYAVELFFAITQHASDWTSAFMPVFFITAFLIMLYLAYQEK
                     IMVYLKEPKEKLSLANRGLSAAERTYVLALVGGMGPKEIAAEYEVSDSTVRNTIARSY
                     QKLGVHDRSGLAALSEKYEVVE"
     gene            complement(5596..6132)
                     /locus_tag="PLG14_06010"
     CDS             complement(5596..6132)
                     /locus_tag="PLG14_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48726.1"
                     /translation="MSSSFPPELWSGLAAQIAAAFRLTPEEARRLEANRTARLVAAIP
                     FAAGCAHPERTALSHLATFLLAGSPSCARAFDHEPSDDADPLLRLAPIADFQGGDHAV
                     IDRGLRLLALVMANGYRKDLAKDQASGEYNPLLSMAWDYEATAGKLRAALEADAKAAP
                     ALDDVMSVEAAVRGFWDA"
     gene            complement(6761..>7356)
                     /locus_tag="PLG14_06015"
     CDS             complement(6761..>7356)
                     /locus_tag="PLG14_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX48727.1"
                     /translation="EGGMTAARSRGAGLFAEGAHRQRRASGFDSLPEPLFLIDGEGRL
                     LEANEAAARLLAALPAEGSDGALGRAELAELFRRAAVHSLAPSPLRVDSPEGARHYEA
                     RFSPAGPGGLKAVLLADITVWKRLLAEKESLLARGRAERGRLVPVCARCGALRDESGA
                     WSGPGALERAGIEPERLTHGLCPACLAKDVARAEPGA"
     assembly_gap    7357..7456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7457..8260)
                     /locus_tag="PLG14_06020"
     CDS             complement(7457..8260)
                     /locus_tag="PLG14_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOX48728.1"
                     /translation="MEQHSRTRAYARFLGASFRANFLAVIEYRANFLLQVFGMMLNNG
                     AFALFWGVLIDRTGGIGGYGFTDVMFVWAVVSSAFGLAHVLFGNIRNLGRIVMQGELD
                     LYLLQPKDVFLNVLASKTIVSAWGDLAYGFIVAALLPGFGPGRLLLFCLLTATGAAIF
                     AATFAAAESLAFFMGNSQAISGAVTEFLLSFSLYPETIFDQGMRWLFYTILPSGFIAF
                     VPLAVYKGLDWRLAPLLVLVATLYAGLSYALFRAGLRRYESGNQMGSRV"
     gene            complement(8264..>9055)
                     /locus_tag="PLG14_06025"
     CDS             complement(8264..>9055)
                     /locus_tag="PLG14_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOX48729.1"
                     /translation="RKYGLAGATGLKTRLAYLGNYAGSILTYGLFVFVFSRIWTAAFD
                     SKAGAAGAIAGYDKAMAIWYFIVAEISVFGFGRFFWRLAEDVKSGQVSYLLGRPYSFV
                     GFHYAESMGPALVDAAIFLVEGLLLGSLLAGPPALLGPGRTAALLLSLLLAGSLQFFL
                     QLAIAMTAFWIEENAAFFWIFQKLVLVVGTLLPIEFLPARAQAAAAFTPFPSLSYAPA
                     RIAVAADPSEGAKLVAVQAAWLLGAVLLCETVYRLARKNLSVNGG"
BASE COUNT         1399 a         2993 c         3355 g         1208 t
ORIGIN      
        1 cgtcggcggg cgccgggccc gccggggtgg gcgacatcgc gccgggcgct gtcgcgccga
       61 ctgcgggcga agccgcgccc ccgcggcctt cggctccgct caaaggtcct ggcccgcgcc
      121 ggcgccgatg cgcttcacgg cgttcacgag gttgaagatg gaggagaagt cgcccgcggg
      181 gatcatgagg ccgaactcca ggcgggaggc ccccgccttg ccctcggcgg cgaggtagga
      241 gtaccaggag ccgaagcggg aggggtcgag gacgacgccc gagtccgcgg ggacggcctt
      301 cgcgatcatc gcggccaggc gcctcgtgga gagggcgccg acgacctgga ccttggcggg
      361 caggcgcttc gcgaaggccg cgaaggcggg atcggcggcg agggagcggg gggcctggct
      421 ccgctcgacc agggccttga gctcggcggg gctcccgagg gtcgcgacga agcggccctt
      481 ggaggtggcg tagcgcaggt tgaagaggcc ggagacggcc tccagggcgg ccctggccgc
      541 ctcggccccg ccctctccct cggaggcggc gaacttctcc tcgtcgagga ccttgaagga
      601 gaagcccatc tcgccgtagg agaagcttcc ctccttccgg tcggcctggc ggatctccat
      661 gccgatgccg tagctctcgc cgaagccctt gaggagggcg gcgacctcgg gatccttcgc
      721 gacgccggag aggagggcgc ggtacttggc ctcgtccttg agctcctgga acatgtccag
      781 gcggatcgtc atggccttct tgatgccgtc ggcgagggcc ttggggtcgt cgccggagga
      841 ttggcccagg gcggtgaggg ccgcgaagtc gaagtccatg tcgagggagg cggcgccgcg
      901 ggggccttgg aggcccagcc acttggcctg gagggcggcg gacttctcga agaaggcctt
      961 gagctgggcg gattcggggc ccatcgagcc tgagagggcg ggcagggcga cgcggaggta
     1021 gaggtcggag agctcagccg cgacgccggg gtcgatggac caggcgtagc cgtagagggc
     1081 gtcggcggag acctggccgg cccaatcgag ggccgagggc gcctgggcgg cgcgggagag
     1141 gaacttctcc atggaggcgc cggggatgac gttggccgcg gccctgaccg tgagcccctc
     1201 ggccccgaag gacagggcgg cgtcggcgga gccgagctcc tttaagaggg ccagggcgcc
     1261 ctcctccaag gccttgaggg cctcctgggg cgagggcgcg gcgccctcct ccacgaagcg
     1321 gcggacgtcc gcctggaagc cggcgaagtc cttcccgccc atgcgctgga gggcgacggg
     1381 gttcgcccag agcttgaccg agtcggcggg gtagcgcgcg aggacggaga ggtccagggt
     1441 ctccgtgggc gggaagacga gggggtactc gccgtcggag tagaccacgt agcccttgga
     1501 ctgggcgagg acctggaatt tgcccgacgc gaggggggcg agggcttcga agtactggcc
     1561 gcggtagggg agatagacga ggctccgcat ctccttggag ccgggggccg cggggacgaa
     1621 ggcgaggacc cagggccggg ccgcgtccag ggccttctcg atgtcggcct cgttcgggag
     1681 gctcttctgg aggagttcgc cgagttcgcc cccggccagg gcggcgacgc ccgcggactt
     1741 ccagaagccc tccagggagg cgtagagctt ggcgggcttc tccaccacga cggcgaggac
     1801 ggcctccttc gggacgaggg ggagggcggg gccggccggc gagccctcta tgcaggagga
     1861 ggagaggagg gcgaggagga tgagggcgac tatggcggcc gctctgggcc gggggcttct
     1921 ggtcatcggg ataccgcctt ggcgcgagga tgcctacata tatagcacgg ggcgccggaa
     1981 agcgcatagc gcggggcctc gccgcgccag gatcgtcgcc gcgccgcgac cgtcccgcgc
     2041 ggcgggcgga agcgcacgcc gcgccgcctc ggccgcggcc tcgacaggga gaccgggccg
     2101 gcctagactc aagaggcggt ccggttccgc gtccgggccg gccaagggag gcgtgatgaa
     2161 ggagctcggc aaggccctcc tctccgagga gcccttctcg gaaatcgtca tcggcgacac
     2221 ggcggtcgcg cgggccctcg tggaggcgcg cgtccgcgtc atctcctcct acccgggctc
     2281 cccgacgccg gagatcgcgg aggccctcgc ggccatcccc aagggggaac ggcccttcta
     2341 cttcgagttc tccgtgaacg agaaggtggc cctggagctg gccttcggcg cggcgatcaa
     2401 cggccacgcc tccgccgtct tcttcaagag cgtcggcctc aacgtcgccg ccgacagctt
     2461 cgtccagctc tccctcatgg acatcccggg gggcctcgtc gtgatcctcg gggacgaccc
     2521 cggcgccaat tcgagccaga acgagcagga caaccgccac tacgcgcggc tcgcctatct
     2581 ccccctcctc gagcccgcga cggcccagga ggcctacgaa atgaccctgg aggcctcgcg
     2641 gctcgcccgg gagcggcgga cggccgtcat cctgcgcctt acgacccacg tctgccacgc
     2701 gaaggagaag gtcgctttcg gcgcctaccg ccccgaaccc ctggaccgga cgccgcgctt
     2761 cgaccccgcg gcctcgaact acatcccgat cgccgcgacg gtcttccccc tgaagcgccg
     2821 cgcccttgag cgcctggagg ccttccgcgg ggacttcgag gcctcccccc tgaaccgcat
     2881 ccttccgggc ggcgaccccc ggcgcggcgt catagccgcg ggcacggccc gcctctccct
     2941 cctcgacgcc ctggaggagc tgcccccgga gcgccgcccc gccgtcctcg agctcgcgac
     3001 ggtccatccc ctgccgcggc gcctggtcgc ccgcttcctc gcggggctcg cggaggcgaa
     3061 gatcctggag gagctcgatc ccctcctcga ggaggagatc aaggccctcg cctacgacga
     3121 ggggctcgcg acgaagatcc tcggcaaggc cgatcccgag gagctcctgg gcgggtacct
     3181 gcccgccaag gtccgcgcca tcctcgcggc cgcctggccc gacctgggcc tcgcgccgcc
     3241 ggcctccgcc ctggccgcgc gggccgccga agccgcggcg ggcgcggcca cggggactgc
     3301 cgcagccgct gcgcgggccc cgcggggccg cgagtccgtg cccgcgcggc cgccccagct
     3361 ctgccccggc tgcggccacc gctccgcctt ccatgcggtc aggaaggccc tccgcgaagg
     3421 ggacatcacg gtggccgaca tcggctgcca caccctgggc cacctgccgc cctacaagat
     3481 gggcacggtc ctcctctcca tgggccactc gacctccacg gcctccggcc tctccctctt
     3541 caacgacagc cgccgcgtcc tctgcttcat cggggactcc accttcttcc acgcggggat
     3601 gccgggcatc gtgaacgcgg tctacaattc gcacaacgtc accctggtcg tcatggagaa
     3661 cgggaccacg gcgatgacgg gccaccagga ccacccgggc gccgggagga actggagcgg
     3721 cgaggcgccg aagataggga tccgcgaggc cctggctggc ctgggcgtcg ccaaggtccg
     3781 ggaggtcgac gcgtacaggc aggccgagct cgaggcggcc gtccgcgagg ccttggccga
     3841 gcccggcttc aaggtcgtca tagcccgcca cccctgcatg ctcaagctcg gacgggaggc
     3901 gaggaggaag ccgggctacg tcccgcgccg cgtcgccgtg gaccagggcg cctgcgccag
     3961 gatccacgcc tgcgtctccg tcttcgcctg cccgagcttc cagctcggga gggacggctc
     4021 ggtggccgtg tcgcgggagc tctgcgtcgg cgacggctcc tgccgccagg tctgccccgc
     4081 gggggcgatc cttccgccgg ccgtcgaggc gcccggaccg gggggtcgcg gggacgaggc
     4141 cggggaagcc gcccgaggcg gcgcgaaggg aggaaagccg tgacggagcg ctacgacatc
     4201 tacatggccg gcgtgggcgg ccagggcatc ggccttcttt cggaggcgct ggcgagggcg
     4261 gccgacaagg ccggcctccc cgtccggggc tgcgacaccc acggcctcgc tcagcggggc
     4321 ggcgtcgtga cgagccacct ccgcctgggc agggccgccc actcgcccct cgtctctccc
     4381 ggcggggccg acctcgtcgt ggcccttgag cgccacgagg ccctgcgcgc ggccagggac
     4441 tatttaaagg agggcggcgt cctcgtctgg tacgacgcgg tcttccagcc cctcgaggtc
     4501 cgcctcggga aggccgccga ggtcggcgcg gcggaggtgg aggaggagtg cgggcggaag
     4561 ggcgcccgcg ccgtcctggt cctccgcgag gacctccccg atccccggat gcagaacgtc
     4621 gccctccttt ccgccctggc cgagctcggc ctcccgccgg gcctccgccg cgagcacctc
     4681 aaggccgcgc tcgaggacct catggagggc gccgccctgg agcggaacct ctccgtcctg
     4741 gagccctgag ggcgccggcg ccccaggcgg cgagcccggc gcctcagccc tgcctgcggc
     4801 gcctcggcga aaaaaaggcg gggccatgcc ggccccgccc ttcccatgga tcggcccgcg
     4861 ctcgggctct acaggctcac tccacgacct cgtacttctc cgacagggcc gcgaggcccg
     4921 agcggtcgtg gacgccgagc ttctggtagg agcgggcgat ggtgttcctg accgtggagt
     4981 ccgacacctc gtactcggcg gcgatctcct tcgggcccat gccgcccaca agggcgagga
     5041 cgtaggtccg ctccgccgcg gagaggcctc ggttggccag ggagagcttc tccttcggct
     5101 ccttcaggta gaccatgatc ttttcctggt aggcaaggta gagcatgatg aggaaggcgg
     5161 tgatgaagaa gacgggcatg aaggccgagg tccagtccga cgcgtgctgc gtgatggcga
     5221 agaatagctc aaccgcatag aggtaggcga ggctgaggac gagccgcagc acacggcgtt
     5281 tctcgtagaa ccccatgcgg aagaggaaga ggacgccgat gacgaaggcg ccgagtccgt
     5341 agaagctctc gcgggactcg atcatcccca ggggcgtcat gaggatgaag gtgaggggtt
     5401 gggcccattg catttggtgc acgaaggcgc tggcaaagaa gatgagcgcc atcgcgaact
     5461 gggtgaccat catccagccg gtcaggaggg gcaatagggg ctcgcgggcg atgatcgcct
     5521 gggcgacatt gagaatgtag ccgagtagga acactccgcc gagcagcagt cccgtggact
     5581 tgaggtaggt cttcatcatg cgtcccaaaa tcccctgacc gccgcctcga cgctcatcac
     5641 gtcgtcgagg gccggggcgg ccttggcgtc ggcctcgagg gcggcgcgga gcttcccggc
     5701 cgtcgcctcg tagtcccagg ccatggagag gaggggattg tactccccgc tcgcctggtc
     5761 cttcgcaagg tccttccggt agccgttcgc catcacgagg gcaaggaggc gcaggccccg
     5821 gtcgatgacc gcgtggtcgc cgccctggaa gtccgcgatg ggcgcgaggc ggaggagggg
     5881 gtccgcgtcg tcggagggtt cgtggtcgaa ggcgcgggcg caggagggcg agcccgcgag
     5941 gaggaaggtc gcgaggtggg agagggcggt ccgctcggga tgggcgcagc cggcggcgaa
     6001 gggaatggcg gcgacgaggc gggctgtcct gttggcctcg aggcggcggg cctcttcggg
     6061 cgtgaggcgg aaggcggccg cgatttgggc ggccaggccg gaccagagct ccggcgggaa
     6121 ggaagacgac atcaaaaacc tccaaccgca ttgtagcatc ccgcggaatt tggtcaatcc
     6181 cgcttcgcgg gccctcttgg gcgcttgtct acgcgctttt tcaggccttt tgggcaattg
     6241 tctacaagca aaacaggccc tttcgcttga tgcgcccccc cgaaagggat aggattttat
     6301 ccgtaatcgg ggctggatct ggccgtacaa cctcttcacc ccggttcatg aagcttgata
     6361 ggtaaaaagc gccgcccgag gcggcaaggt aaaaaagtgc cggttctgcg accgggcagc
     6421 acaaaatcct gaaaagccct cggggctttg aggtaaaaag aagccggccg aagaggccgg
     6481 aaggtaagaa caggcacaaa aatcggggag ttaaaaaatg cgcggagggc gtcccggagg
     6541 ggacgccctt cgtgctttta aatgctagtt ccgttggccg ggcggctttc cgggacgggc
     6601 gcggaggccg cgcttactgc gccgactgcg ccatcgccgc cgcgccgcct ccctcgcgcc
     6661 gcttctctcc cctcgcgccg cttcgactgc ccccgcgccg acctgctcgg gcagccctag
     6721 gctcgaggcc ggcgaccggc ctggtccggg aggctcgggc ctaggccccc ggctccgccc
     6781 gcgccacgtc cttcgcgagg caggcggggc agaggccgtg ggtcaggcgc tcgggctcaa
     6841 tgccagcccg ctccagggcg ccggggccgc tccaggcgcc cgactcgtcc cgcagggccc
     6901 cgcagcgggc gcagacgggg acgaggcgcc cgcgctcggc cctgccgcgc gcgaggaggc
     6961 tctccttctc cgcgaggagg cgcttccaga ccgtgatgtc ggcgaggagg acggccttga
     7021 ggccgcccgg gccggcgggg gaaaaacgag cctcgtagtg gcgcgcgcct tcgggcgagt
     7081 ccacgcggag cggcgagggc gcgaggctat ggacggccgc ccgcctgaag agctcggcga
     7141 gctcggcgcg gcccagggcc ccgtccgagc cctccgccgg aagggcggcg aggagccgcg
     7201 ccgccgcctc gttcgcctcc aggagtctgc cctcgccgtc gatgaggaag aggggctcgg
     7261 gcaggctgtc gaagccgctc gcgcggcgct gccgatgagc cccttccgcg aagaggccgg
     7321 cgccccggct cctcgcggcg gtcattcctc cttcgtnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnctag accctcgacc ccatctggtt ccccgactcg tagcgcctga
     7501 ggcccgcccg gaagagggcg taggagagtc cggcgtagag ggtcgcgacc aggacgagga
     7561 ggggagcgag ccgccagtcc aggcccttgt agacggcgag gggcacgaag gcgatgaagc
     7621 ccgagggcag gatggtatag aagagccaac gcatgccctg gtcgaagatc gtctccgggt
     7681 agagggagaa ggagaggagg aactccgtga ccgcgcccga gatcgcctgg gagttgccca
     7741 tgaagaaggc caggctctcc gcggccgcga aggtggcggc gaagatcgcc gcccccgtgg
     7801 ccgtgaggag gcagaagagg aggaggcgtc cggggccgaa gcccgggagg agggcggcga
     7861 cgatgaagcc gtaggccagg tcgccccagg cggagacgat ggtcttggac gcgaggacgt
     7921 tgaggaagac gtccttgggc tggaggaggt agaggtcgag ttcgccctgc atgacgatcc
     7981 gccccaggtt cctgatgttc ccgaagagga cgtgggcgag gccgaaagcg gaggagacga
     8041 cggcccagac gaacatcacg tccgtgaagc cgtagccgcc tatgcccccc gtgcggtcga
     8101 tgaggactcc ccagaagagg gcgaaggccc cgttgttgag catcatcccg aagacctgga
     8161 ggaggaagtt ggcccggtac tcgatcacgg ccaggaagtt ggccctgaag gaggcgccca
     8221 ggaagcgggc gtaggccctg gtcctgctgt gctgttccat gtgtcagccc ccgttcacgc
     8281 tgaggttctt tcgcgcgaga cgatagacgg tctcgcagag gaggacggcc ccgaggagcc
     8341 aggcggcctg gacggcgacg agcttcgcgc cctcggacgg gtcggcggcg accgcgatgc
     8401 gggccggcgc gtaggagagg gaggggaagg gcgtgaaggc cgccgccgcc tgagcccgtg
     8461 ccgggaggaa ctcgatgggg aggagggtcc cgacgaccag gacgagcttc tggaagatcc
     8521 agaagaaggc cgcgttctcc tcgatccaga aggccgtcat cgcgatcgcg agctggagga
     8581 agaactggag cgagcccgcg aggaggaggg agaggaggag ggcggccgtc cgccccgggc
     8641 cgaggagggc cggcggcccc gcgaggaggc ttccgaggag gaggccttcg acgaggaaga
     8701 tggccgcgtc caccagggcg gggcccatcg actccgcgta gtggaagccg acgaagctgt
     8761 agggccgccc gaggaggtag gagacctggc ctgacttcac gtcctctgcg aggcgccaga
     8821 agaagcgccc gaagccgaag acggagatct ccgcgacgat gaagtaccag atggccatgg
     8881 ccttgtcgta gcccgcgatc gcgccggcgg ctccggcctt ggagtcgaag gccgccgtcc
     8941 agatgcgtga gaagacgaag acgaagaggc cgtaggtgag gatggagccc gcgtagttcc
     9001 cgaggtaggc caggcgggtc ttgaggcccg tcgcccccgc gaggccgtac ttcct
//
LOCUS       DAOOSJ010000093         9053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4886_length_9053_cov_6.890642, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000093 DAOOSJ010000000
VERSION     DAOOSJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4886_length_9053_cov_6.890642"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..477
                     /locus_tag="PLG14_06030"
     CDS             52..477
                     /locus_tag="PLG14_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOX48730.1"
                     /translation="MKAHCFRLTRGQDLLLEIEAYARARGLKAAAVVSAVGCVSRARL
                     RDASGVTLRDLDEPLEIVSLMGTVSAARTHLHASYSREDLSTLGGHLVPGSVVNTTAE
                     VVLLELPGFEFGSAFDEETGYEELVISGAAQAPHPRSDP"
     gene            474..914
                     /locus_tag="PLG14_06035"
     CDS             474..914
                     /locus_tag="PLG14_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48731.1"
                     /translation="MKLIYRLQLVIHLVVGLGALAGGYGAVSDPYAPMGIPAAALEKG
                     PFTSFLVPGLFLLVVLGLGNLVSAFLAAKKSRLSGYASGAMGAALAAWIVVQCWILQS
                     IVALHAIFFAVGAVQGLLALALLWLRGDWPLGLLKERLAAKSAR"
     gene            complement(1134..3086)
                     /locus_tag="PLG14_06040"
     CDS             complement(1134..3086)
                     /locus_tag="PLG14_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOX48732.1"
                     /translation="MKKVSRILSAALAVALAMGLAGCEGLKGSTKDTAQAPVARAAAT
                     AMKAGVYTQAAEGMHGPLVVEVTLSERKIEKVAIKSQNETAGLSDLALAELPGRIVAA
                     QSVAIDAVSGSTMTSNAILAAVGKAIEAAGGKVDEFMVKDEPKTAAKAPEAEREPALA
                     SALPASWDLSYDVVVVGGGFAGLAAAQSAATHGAKTVLVEKMPFVGGNSQINGGVYAA
                     YTSKLAADFQKKFNLVPDTAEKHIQDTLKGGDYMGDPALVKNLVYGSPFYLDMLLSNG
                     LKVRESLTRPGGHYGYRTYTTVNGVGADIVQVQKKMVKEAGVEVLTLTKMTKIYREAK
                     GEGRVVGIQVETKDGLKTIRAKKGLILATGGFSANVEMRQKQVPTLNATLPTTNHVGA
                     TGEGISMAQEIGANTIQMSYIQLYPFANPNNGVLDAFAVIPFSGPSAGIVYVDVNGKR
                     YVNEGERRDVCSKAAQDSAGFPTFSIFGEEIYRKGGFITEGQMADGIKADRIFKADTL
                     EELAAEINKRAYKGGKINMSGAVLAQTIATHNGYVAALKDPDFGKRIDKGVMMTIEKG
                     PYYAIPQWPSVHHTMGGLAITPKTEVQDVWGKVIPGLYAAGEVTGGVHGTNRLGSNAI
                     PDACVHGYIAGEVAADGTLPAFIQGK"
     assembly_gap    3662..3761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3762..4301
                     /locus_tag="PLG14_06045"
     CDS             <3762..4301
                     /locus_tag="PLG14_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOX48733.1"
                     /translation="DALAAWRGLLASGFAPRDLVVAGDSAGGGLSLALVLSLRDSGEP
                     LPSAVVCVSPWTDLAASGGSRAEKAGAEAILSVARLDEWALRYAGSAGLDHPLVSPLR
                     AELRGLPPLLVQAGSEEILLDDSVKLAAKAEAAGVEARLSVWPGLFHAWPALGGLLPE
                     SALAFAEARGFILAVEARP"
     gene            4518..5708
                     /locus_tag="PLG14_06050"
     CDS             4518..5708
                     /locus_tag="PLG14_06050"
                     /inference="COORDINATES: protein motif:HMM:NF008887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOX48734.1"
                     /translation="MQRHVIGNVYWVGKIDWELKKFHGSEYSTHKGSSYNSYLLREGK
                     TVLVDTVWLPFAKEFVDNLEAEVGLANLDFVVQNHAEIDHSGALPELLRRRPDLPIYC
                     TANAVKSLKGHYHQDWDFRVVKTGDSVDVGNGKKLVFVEAPMLHWPDSMFTYLAGEAV
                     LFPNDAFGQHYASEFLFNDLVDQDELWDECVKYYANILTPFSALVAKKIEEVVGLKLP
                     VSVIAPSHGVIWRKDPLQIVTKYAQWAADYKEDRVAILYDSMWDGTRKLAEAMAQGVA
                     EASPSTSVVVHNVAKTDKNDVITDVFRSKALLLGSPTINKGVLHAVAGVLEEIRGLRF
                     KGKKAAAFGCYGWSGESPKLLSEELAKAGFEVVDEGHKCLWEPDAEAIAAAREYGRQF
                     ARKI"
     gene            5762..>6122
                     /locus_tag="PLG14_06055"
     CDS             5762..>6122
                     /locus_tag="PLG14_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX48735.1"
                     /translation="MSKAVEDLMHEHEAILFTLRVLDKMAGLAEAGKDPGREDALALI
                     AFLREFADKCHHGKEELILFPALEAAGIRNSGGPIGQMLAEHDAGRALVGGMAAALER
                     PDGFPAFARAARDYAALL"
     assembly_gap    6123..6222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6330..9050
                     /locus_tag="PLG14_06060"
     CDS             6330..9050
                     /locus_tag="PLG14_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48736.1"
                     /translation="MEEIDMATRPLIMKLARKISIESLTYTGITAKDPEYRILDPVVT
                     DEMAAVGLGLKVRKYLTTPEVAKSVGKPEAHCAEQLWKLADAGVVKVAKGKDGQDTWF
                     LPIWVPGIMECMVANKANAEKFPVLGECFEEYTRRRTKILAPNLPVGKGLMRVIPVEK
                     AIDGETVARSYEEVSKYVENAYMLAVSDCTCRRSRRLMGEGCGHLETDMCIQLDDGAE
                     FFVRTGKARQITKEEAYEILKRAEDNGLVHQTPNLDGPGNTLAICNCCGCSCFALRVG
                     EYYNAFDFNRSNYVSKIDKDKCVACGRCVENCQVNALKLGQKLCSKTPVVTEFRHLHP
                     KDNVWGRDKWNPGFRTSRQNVVETGTAPCKTNCPAHLGIQGYVKLASQGKYREALELI
                     KQDNPFPAVCGRICNRRCEEACTRGELDEAVAVDEIKKFIAQRDLDAKTRYVPPMLNT
                     IGKPYPNKVAVVGAGPAGLSCAFYLARQGYPVTVFEKEKKLGGMMTLGIPSFRLEKDV
                     VEAEIDVLREMGVAFRTGVEVGKDVTLDSLRKEGFEAFYVAPGAQGGRKIGVPGEEAA
                     GVAAGVDFLRAVNLGEGPKLAGKVLVIGGGNVAVDVARTAVRSGAASVSMFCLETRDI
                     MPAAEDEIEEAEAEGIDIQNAWGPKEILAEGGKVKAVVFKKCVAVFDAEGKFAPRYDE
                     AETRTVDCDAVLLSVGQSIQWGALLAGSKVELNRNGTVKADAFTYQTAEPDVFVGGDA
                     LTGPRFAIDAIAAGKEGAVSIHRFVQPGQSLVIGRDRKAYKELDRKSAVLGSYDRAPR
                     QKPEGGAAAKAKATFRDLRGTFTEEQLKKETERCLSCGATIVDEYMCVGCGICTTKCA
                     FDAVRLVRRSDVAGVPFEKEVLGVMVPNLLKRGGRIAFKPVAKLFAKGAE"
BASE COUNT         1373 a         3006 c         3080 g         1394 t
ORIGIN      
        1 ctattccccg gggcaaggca gtcgaggtat acttctcctt gaggtgcgca catgaaagcc
       61 cactgcttcc gcctgacgcg cggccaggac ctcctcctcg agatcgaggc ctacgcccgc
      121 gcgcgcgggc tcaaggccgc cgcggtcgtc tccgccgtcg gctgcgtctc cagggcgcgg
      181 ctccgcgacg cgagcggggt gacgctgcgc gacctggacg agccgctcga gatcgtctcc
      241 ctcatgggca cggtctccgc cgcgaggacc cacctccacg cctcctatag ccgcgaggac
      301 ctctcgaccc tcggcggcca cctcgtgccc ggctccgtcg tcaacacgac ggcggaggtc
      361 gtcctcctcg agcttcccgg cttcgagttc ggctccgcct tcgacgaaga gacgggctac
      421 gaggagctcg tgatcagcgg cgcggcgcaa gccccgcatc caaggagtga cccatgaagc
      481 tgatctaccg cctccagctc gtcatccacc tcgtcgtggg cctgggcgcc ctcgcgggcg
      541 gctacggcgc cgtctccgat ccctacgcgc ccatgggcat tcccgcggcc gcgctcgaga
      601 aggggccctt cacgtccttc ctggttccgg gactcttcct cctcgtcgtc cttgggctcg
      661 ggaacctcgt ctccgccttc ctggccgcga agaagtcccg cctctcgggc tacgcaagcg
      721 gcgcgatggg cgcggcccta gcggcctgga tcgtcgtcca gtgctggatc ctccagtcga
      781 tcgtcgccct ccacgcgatc ttcttcgcgg tcggcgccgt ccagggcctc ctcgccctgg
      841 ccctcctctg gctgcggggc gactggcccc tgggcctcct caaggagagg ctcgccgcga
      901 agagcgcgag gtaggcggac cgcagccttc ctgcccgcgt ggcgcgcgag ccgggcggtc
      961 cgctgccagt ggcggtccgc gaggcgcggg ctaggcagct tgatgcggca gatcgcggcc
     1021 gaccgcgtga aatgagagag ccccgccccc ggtcgcccgg gagcggggct ctacgttttt
     1081 gcctggccgc gccgccgtga gccttggctc cgggcggcgg cgcgggggag ggcttacttg
     1141 ccctggatga aggcggggag ggtcccgtcg gcggcgacct ctccggcgat gtagccgtgc
     1201 acgcaggcgt cggggatcgc gttggagccg aggcggttcg tgccgtgcac gccgcccgtc
     1261 acctcgcccg cggcgtagag gccggggatg accttgcccc agacgtcctg gacctcggtc
     1321 ttcggggtga tcgcgagtcc gcccatcgtg tggtggaccg agggccactg ggggatggcg
     1381 tagtaggggc ccttctcgat ggtcatcatg acgcccttgt cgatgcgctt cccgaagtcg
     1441 gggtccttga gcgcggcgac gtagccgttg tgggtcgcga tggtctgcgc caggacggcg
     1501 ccggacatgt tgatcttgcc gcccttgtag gcgcgcttgt tgatctccgc ggcgagctcc
     1561 tcgagcgtgt ccgccttgaa gatgcggtcg gccttgatgc cgtcggccat ctggccctcg
     1621 gtgatgaagc cgcccttgcg gtagatctcc tcgccgaaga tggagaaggt cgggaagccc
     1681 gcggagtcct gggcggcctt ggagcagacg tcgcggcgct cgccctcgtt gacgtagcgc
     1741 ttgccgttca cgtccacgta gacgatgccg gcggaggggc cggagaaggg gatgaccgcg
     1801 aaggcgtcga ggacgccgtt gttcgggttc gcgaaggggt agagctggat gtagctcatc
     1861 tggatcgtgt tcgcgccgat ctcctgggcc atggagatgc cctcgccggt ggcgccgacg
     1921 tggttcgtgg tcggaagggt ggcgttgagg gtcgggacct gcttctggcg catctcgacg
     1981 ttggcggaga agccgccggt cgcgaggatg aggcccttct tggcgcggat ggtcttgagg
     2041 ccgtccttgg tctccacctg gatgccgacg accctgccct cgcccttggc ctcgcggtag
     2101 atcttcgtca tcttggtgag ggtcaggacc tcgacgccgg cctccttcac catcttcttc
     2161 tggacctgga cgatgtcggc gcccacgccg ttcacggtgg tgtaggtgcg gtagccgtag
     2221 tgcccgccgg gcctcgtgag ggactcgcgg accttgaggc cgttggagag gagcatgtcg
     2281 aggtagaagg gcgagccgta gacgaggttc ttcacgagcg ccgggtcgcc catgtagtcg
     2341 ccgcccttga gggtgtcctg gatgtgcttc tccgcggtgt cggggacgag gttgaacttc
     2401 ttctggaagt ccgcggcgag cttggaggtg taggccgcgt agacgccgcc gttgatctgg
     2461 gagttgccgc cgacgaaggg catcttctcc acgaggacgg tcttggcgcc gtgggtcgcg
     2521 gcgctctgcg cggccgcgag gccggcgaag ccgccgccca cgacgacgac gtcgtagctc
     2581 aggtcccagg aggcggggag ggcggaggcg agggcgggct cgcgctcggc ctcaggggcc
     2641 ttggccgcgg tcttgggctc gtccttgacc atgaactcgt cgaccttgcc gccggcggcc
     2701 tcgatggcct tgccgaccgc ggcgaggatc gcgttgctcg tcatcgtgga gccggagacg
     2761 gcgtcgatcg cgacgctctg ggccgcgacg atgcggccgg ggagctcggc gagggcgagg
     2821 tcggagaggc ccgcggtctc gttctgggac ttgatcgcga ccttctcgat cttcctctcg
     2881 gagagggtga cctcgacgac gagggggccg tgcatgccct ccgccgcctg ggtgtagacg
     2941 ccggccttca tcgcggtggc cgccgctcgg gcgacgggcg cctgggccgt gtccttggtc
     3001 gagcccttga ggccttcgca gcccgcgagg cccatggcca gggcgaccgc gagggccgcc
     3061 gacaggatcc tggaaacctt cttcacgtgt tcctccttgg agacaagtga cctggatctc
     3121 gccccttaaa gcctccggcc aaggggcgga tcatggatga gacggtatgg gcgatgtccc
     3181 gcctggcttc atgcctggct ggtccttcgg tcttcgctgg ggtcgtgagg cgcgatgagg
     3241 atgaggcgtg cgtctgtggg cctgagtgga tcgaaggcgg gccggcgctc gaggccggct
     3301 actgttcgcg cgatgcccgc tctcgagagc ggggcgcgtt ggctgtttct gaagaaaatc
     3361 ttatccattg atccgaggac tgtcaacgaa aaaagggaga aaaatacctg aatattagtg
     3421 aacattcgtg cgatggcccg cctgtgaatc gatacgacgc gcgccgtgac gaggatataa
     3481 cgcgtcgcgc ggcgcggcga cggcgcgtcg ggtcgcggcg cgaggcgcgc cggccggcga
     3541 cgagggcgcc gcgggcgttg acaagcggcc ccgatgcgct agccttggcg tgggggtgaa
     3601 gcatgcgtcc cagtctccgc gcgcgcttct ggcgcgccgt cctccgcaag gccgtgaagg
     3661 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacgcgctc gcggcctggc
     3781 gcggcctcct cgcctcgggc ttcgcgccgc gggacctggt cgtcgccggc gactccgcgg
     3841 gcggcggcct ctccctcgcc ctcgtccttt cgctcaggga ctctggcgag cccctgccct
     3901 cagcggtggt ctgcgtctcg ccctggaccg acctggcagc ctcgggcggg agccgagccg
     3961 agaaggccgg cgcggaggcg atcctgagcg tcgcgcggct cgacgagtgg gccctgcgct
     4021 acgcggggag cgccggcctc gaccatcccc tggtctctcc gctccgcgcc gagcttcgcg
     4081 gcctgccgcc ccttctcgtc caggccggca gcgaggagat cctcctcgac gactccgtga
     4141 agctggccgc caaggcggag gccgccggcg tggaggcgcg gctctccgtc tggcccggcc
     4201 tcttccacgc ctggccggcc ctgggcgggc tcctccccga gagcgctctc gccttcgcgg
     4261 aggcgcgcgg cttcatccta gccgtggagg cgcggcccta gcggcgcggc tccagcggcg
     4321 gcgcctcggc ccgggcaccg gctcgcccag gtcgccgctc tggccgccgt gctggtggcc
     4381 gccttggccg ccctggcttt cccgcgattc cgtaaccaag gttacggcaa acttccggcc
     4441 aaggcgcgat acttgtactc gaggccgcgg ggccgggaga ccggcgcccg cgaccgatca
     4501 agaggcaggg ggtcgccatg cagcgccacg tgatcggaaa cgtctattgg gtgggcaaga
     4561 tcgactggga gctcaagaag ttccacggga gcgagtactc gacccacaag ggctcgagct
     4621 acaactccta cctcctccgc gaggggaaga cggtcctcgt cgacaccgtc tggctgccct
     4681 tcgcgaagga gttcgtcgac aacctggagg ccgaggtggg cctcgccaac ctcgacttcg
     4741 tggtccagaa ccacgcggag atcgaccact cgggcgccct gcccgagctc ctccgccgca
     4801 ggcccgacct cccgatctac tgcaccgcga acgcggtgaa gagcctgaaa gggcactacc
     4861 accaggactg ggacttccgc gtcgtgaaga cgggcgactc cgtcgacgtc gggaacggga
     4921 agaagctcgt cttcgtggag gcgccgatgc tccactggcc cgactcgatg ttcacctacc
     4981 tggccggcga ggccgtcctc ttcccgaacg acgccttcgg ccagcactac gcctcggagt
     5041 tcctcttcaa cgacctcgtg gaccaggacg agctctggga cgagtgcgtg aagtactacg
     5101 cgaacatcct cacgcccttc tccgccctcg tggcgaagaa gatcgaggag gtcgtgggcc
     5161 tcaagctccc cgtcagcgtg atcgctccca gccacggcgt catctggcgg aaggacccgc
     5221 tccagatcgt gacgaagtac gcgcagtggg cggccgacta caaggaggac cgcgtcgcga
     5281 tcctctacga ttccatgtgg gacgggacga ggaagctcgc ggaagcgatg gcccagggcg
     5341 tcgccgaggc gagcccctcg acctccgtcg tcgtgcacaa cgtcgcgaag acggacaaga
     5401 acgacgtcat caccgacgtc ttcaggtcca aggccctcct cctcggctcg cccacgatca
     5461 acaagggcgt cctgcacgcg gtggccggcg tcctggagga gatcaggggc ctccgcttca
     5521 aggggaagaa ggccgcggcc ttcggctgct acggctggtc gggcgagtcg cccaagctcc
     5581 tctccgagga gctcgcgaag gccggcttcg aggtcgtgga cgagggccac aagtgcctct
     5641 gggagcccga cgccgaggcg atcgcggccg cgcgggagta cggccgccag ttcgcgagga
     5701 agatctagcc gcgcggccgc gaccgcgaga cggtcgcggc ccccgcgccg aaggagaagc
     5761 catgagcaaa gctgtcgaag acctcatgca cgagcacgag gcgatccttt tcacgctccg
     5821 cgtcctggac aagatggccg ggctcgcgga ggcggggaag gacccgggga gggaggacgc
     5881 cctcgccctc atcgccttcc tcagggagtt cgccgacaag tgccaccacg ggaaggagga
     5941 gctcatcctc tttcccgccc tggaggcggc gggcatccgg aactcaggcg ggcccatagg
     6001 gcagatgctc gcggagcacg acgccgggag ggccctggtc ggcggcatgg ccgcggcctt
     6061 ggagaggccc gacggcttcc ccgccttcgc ccgggcggcc agggactacg ccgccctcct
     6121 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgcccac aatacgcaag
     6241 cctggcgcgg ctaaacgcgc gcgatgcgtg atattcctgg acaactcggg atacccggtg
     6301 tacagtccaa aaactaaaat atcgatacgt tggaagagat agacatggcc acgagacctt
     6361 tgatcatgaa gctcgcgagg aagatcagca tcgagtcgct gacctacacc ggcatcacgg
     6421 cgaaagatcc cgagtaccgg atcctggacc cggtcgtcac ggacgagatg gccgccgtgg
     6481 gcctcggcct caaagtcagg aagtacctca cgacgcccga ggtcgcgaag agcgtgggca
     6541 agcccgaggc ccactgcgcc gagcagctgt ggaagctcgc cgacgccggc gtcgtcaagg
     6601 tcgcgaaggg caaggacggc caggacacct ggttccttcc catatgggtg ccgggcatca
     6661 tggagtgcat ggtcgccaac aaggcgaacg ccgagaagtt cccggtcctg ggcgagtgct
     6721 tcgaggagta cacgcgcagg cggacgaaga tcctcgcgcc caacctgcct gtcggcaagg
     6781 gcctcatgcg cgtcatcccg gtggagaagg ccatcgacgg cgagaccgtc gcccgcagct
     6841 acgaggaggt ctccaagtac gtggagaatg cctacatgct cgcggtctcc gactgcacct
     6901 gccgccgctc ccgccgcctc atgggcgagg gctgcggcca ccttgagacg gacatgtgca
     6961 tccagctcga cgacggcgcg gagttcttcg tccgcaccgg caaggcgcgc cagatcacca
     7021 aggaagaggc ctacgagatc ctgaagcgcg cggaggacaa cggcctggtc caccagacgc
     7081 cgaacctgga cggccccggc aacacgctcg ccatctgcaa ctgctgcggc tgctcctgct
     7141 tcgcgctccg cgtcggcgag tactacaacg ccttcgactt caaccgctcc aactacgtgt
     7201 cgaagatcga caaggacaag tgcgtcgcct gcggccgctg cgtggagaac tgccaggtca
     7261 acgccctcaa gctcggacag aagctctgct cgaagacgcc ggtcgtcacg gagttccggc
     7321 acctccaccc gaaggacaac gtctggggcc gcgacaagtg gaaccccggc ttccgcacgt
     7381 cccgacagaa cgtcgtggag acggggaccg cgccctgcaa gaccaactgc ccggcgcacc
     7441 tgggcatcca gggctacgtg aagctcgcct cccagggcaa gtaccgcgag gccctggagc
     7501 tcatcaagca ggataatccc ttccccgccg tctgcggccg catctgcaac cgccgctgcg
     7561 aagaggcctg cacccgcggc gagctggacg aggccgtcgc ggtggacgag atcaagaagt
     7621 tcatcgccca gcgggacctg gacgcgaaga cgcgctacgt gcccccgatg ctcaacacga
     7681 tcggcaagcc ctacccgaac aaggtcgccg tcgtcggcgc cggccccgct ggcctctcct
     7741 gcgccttcta cctcgcgcgg cagggctatc ccgtcaccgt cttcgagaag gagaagaagc
     7801 tcggcggcat gatgaccctg ggcattccct ccttccgcct ggagaaggac gtcgtggagg
     7861 cggagatcga cgtcctgcgc gagatgggcg tggccttcag gaccggcgtc gaggtcggca
     7921 aggacgtgac gctggactcg ctccgcaagg agggcttcga ggccttctac gtcgcgccgg
     7981 gcgcccaggg cggccgcaag atcggcgtgc cgggcgagga ggccgcgggc gtcgccgccg
     8041 gcgtcgactt cctgcgcgca gtgaacctgg gcgagggccc gaagctcgcg ggcaaggttc
     8101 tcgtcatcgg cggcggcaac gtcgccgtcg acgtggcgcg gaccgccgtc cgctccgggg
     8161 cggcctccgt ctccatgttc tgcctggaga cccgcgacat catgcccgcg gcggaggacg
     8221 agatcgagga ggccgaggcc gagggcatcg acatccagaa cgcctggggc ccgaaggaga
     8281 tcctcgccga gggcggcaag gtgaaggccg tcgtcttcaa gaagtgcgtc gcggtcttcg
     8341 acgccgaagg caagttcgcg ccccgctacg acgaggctga gacccgtacc gtggactgcg
     8401 acgccgtcct cctctccgtg ggccagtcga tccagtgggg cgccctcctc gcgggcagca
     8461 aggtcgagct caaccgcaac ggcaccgtga aggcggacgc cttcacctac cagaccgccg
     8521 agcccgacgt cttcgtcggc ggcgacgccc tcaccggtcc ccggttcgcc atcgacgcga
     8581 tcgcggccgg caaggaaggc gccgtctcca tccaccgctt cgtccagccg ggccagagcc
     8641 tggtgatcgg ccgcgaccgc aaggcctaca aggagctcga caggaagagc gccgtgctcg
     8701 ggagctacga ccgggcgccc cgccagaagc ccgagggcgg cgccgccgcc aaggccaagg
     8761 ccaccttccg cgacctccgc ggcaccttca cggaggagca gctgaagaag gagacggagc
     8821 gctgcctctc ctgcggcgcc acgatcgtcg acgagtacat gtgcgtcggc tgcggcatct
     8881 gcaccaccaa gtgcgccttc gacgcggtgc gcctggtcag gcggagcgac gtggcgggcg
     8941 tgcccttcga gaaggaggtc ctgggggtca tggtgcccaa cctcctgaag cgcggcggca
     9001 ggatagcctt caagccggtc gcgaagctct tcgcgaaggg cgccgagtag gga
//
LOCUS       DAOOSJ010000094         9049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4888_length_9049_cov_6.736602, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000094 DAOOSJ010000000
VERSION     DAOOSJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_9049_cov_6.736602"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLG14_06065"
     CDS             complement(<1..990)
                     /locus_tag="PLG14_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX48737.1"
                     /translation="MERGRYFEFHNPVKILAGEKALDSLPNELERLGCRRPLLVADKG
                     VRQAGLVDVLLRAAGESSLAFAAVFDDVPPDSGTRVVAAAARAYREAACDGIVAVGGG
                     SVIDTAKSVNVLVTKGGDDLVAYSGAGAIAGRLSPFVVVPTTAGTGSECTLVAVVRDD
                     ERDRKLLFVSASLLPDAAVLDPRMTLTLPPVVTAATGMDALTHAVEASYCLGKNPLSD
                     VYADTAIRLVSERIVHVTKNPGDARGRLDLAVASTMAGIAFSNSMVGLVHSLGHATGG
                     VCHVPHGVAMNVFLPHVLEYNLGKRGAEIGRLLLPLSGPEAYYSTPEARRPEAA"
     gene            complement(993..1559)
                     /locus_tag="PLG14_06070"
     CDS             complement(993..1559)
                     /locus_tag="PLG14_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48738.1"
                     /translation="MDDGTRRPAAGLPPPALTPPGRPLAKAYAALALGLVGRAFAVAH
                     RVDPGLRRELADLPPGFSFALAVHPKGPRAALRMGEDGRLRYLGRGPESPDVLMRFQN
                     LSAALAVLTFSASAFEVYARNGLAVSGDLPSTMAIIRALGLVESLILPRFVVARVLKR
                     VPSAPFARKHAERALIYLALPFAGPTKE"
     gene            complement(1602..2195)
                     /locus_tag="PLG14_06075"
     CDS             complement(1602..2195)
                     /locus_tag="PLG14_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX48739.1"
                     /translation="MAAVNPDADSRDLILAAAEAVVSERGVEAASLADIAKAAGVSRG
                     TLYYHYPSKDALVLDIAERHMAALTDRIFELARSEGASGLPLMLEALVREILADDTRS
                     ATHVHLVQCAFEGNEAVRERMAASYRGWIDMVVEELARLGLASRKDEEVGSLVVAVLD
                     GLVMQKRILGRGSRISESRVAALLSVALGEPARQAAP"
     gene            complement(2195..4201)
                     /locus_tag="PLG14_06080"
     CDS             complement(2195..4201)
                     /locus_tag="PLG14_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOX48740.1"
                     /translation="MKTIVGESGRCLDIDLSSRSYRVFSPAEEDLLGYLGGKGLGTKL
                     YYDRLGGADGTALREVDPLGPENILAFMMGSFAGTGAPCSARFAGVTKSPLTGIMVSS
                     SCGGPFGLACKTAGWDGILVSGRASSPLVLRIDEDGVRFEEAEGLWGLETGPAQERLV
                     DNPRQGALVIGPAGENGVLYAVIRSGNRYLGRGGMGAVMGAKNLKAVVATGRAYRIEP
                     ADPERYARLNAKARKHINRNPFVLSYRAYGTNYGVNPGVDRGYAPVRNFRDRTDERCR
                     SLSGEAMAERYATEHAACAPCAVLCGHKGTYPDGQKRHIPEYETIGLWGGNIMNFDPD
                     LVAVWNDRMGELGLDTISCGATASWAMEAAEKGLRPSELAFGRTDNIARILDDIAHRR
                     GEGAELALGSRRLAEKYGGLEFAAQVKGMEMAAYDPRGGWGQGLNYAVANRGGCHLNA
                     YPIALEAIFGFIPQYSRLSKASWVDFFEDLFTAVNSTQTCQFSAFGYLLEPPVAKYTP
                     KPLLKLAMTLLPEVAQSMLDWSCLSGLVGAITGRRVTPRDFRMAGRRAHVLERRMNCL
                     MGVTAADDTLPGRFLTEGKTRHPVDSVVPIEPLVKAYYRKKGYGPDGVPTARTLARLG
                     IGAPAGSRDASRAAAADRGAPVGPEAAGAAADGAAPATQAAGRP"
     gene            complement(4198..4638)
                     /locus_tag="PLG14_06085"
     CDS             complement(4198..4638)
                     /locus_tag="PLG14_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX48741.1"
                     /translation="MAHSIGPACIGCAACVRVCPVAAISGERGSRHAIARPRCVECGA
                     CGRVCPKSAVLDEGGAAVARLMPRALWAKPVFELSRCISCGACAQKCPASCLAMGKGR
                     PGGLGAYPSLAAPEACVSCGYCAFYCPMDCITIAAPGGAEEAAS"
     gene            complement(4763..6106)
                     /locus_tag="PLG14_06090"
     CDS             complement(4763..6106)
                     /locus_tag="PLG14_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOX48742.1"
                     /translation="MSEYLTRDPGKQARLKEIIGRLHGGATVAEVKRDFDTLIKGVSA
                     EEVAAMEQALVDEGFPVEEIQRLCEVHVAVFESSLEKGHKPSRMPGHPVHSFMAENRE
                     AERLLGRLLRGARAWAWGLGSGSSEAGAALGELAKLAVHYARKENQLFPYLERHGFTG
                     PSKVMWGKHDEIRVLLKEAEAAYREASGSEGARRFRSAARKLAAAVRRMVFMEERILY
                     PNALRRLSEREWAEIRKGEEAIGFAWIEPGAAYDADLVLARLASGSGAGAAARTGDGE
                     SAAPGGGPASAPGAAPVGAPASGGASHAPGGAAPQAGLLELNTGRIGLEVLDLALRAL
                     PLDFSVVDENDRVLYYSDAPERIFPRSPAVIGRAVQNCHPQKSVAVVESILSAFKKKE
                     KSQARFWIEMGGKFVVIEYRALYDQAGRYRGCLETTMDAAPYRGLQGERRLLDWS"
     gene            complement(6238..7728)
                     /locus_tag="PLG14_06095"
     CDS             complement(6238..7728)
                     /locus_tag="PLG14_06095"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOX48743.1"
                     /translation="MDARAEQGPKPDTSLISDENERLRRENEALGQRVAELERKLSLQ
                     PQTGLPTHFRLELELDDLIDTARRESPSRGFAVLIVQLGENYGVVRKTLKASVSEWIL
                     YQTGCRITALLREEDRLFHTHESEFVLLLPGMKGEDLAAFLRRLLPRLNEPHIFAGFN
                     VAVGAYSGAAYWPEHGEERSVLLHHADIAVGASVEARKAFLLFRPPLLEKAVEKVELQ
                     NSIIKAIEATALDRIGEQFFLCFQPKLFARGLEDGSLLVDRIEAEALIRWRHPERGIL
                     MPSTFVPLAEETGLILPLGKWLLYQCARRLAAWAKSGRPEIGLSVNLSARQFKSEDAA
                     ETLAAVLAYTGAKPDLLTVELTETSLFEDPTATTATLERFAALGVRISVDDFGTGYSS
                     LSHLHRFPLDEIKIDRLFVENLDGNRQDRIIVRSLVAIAKGLDLSLVAEGVEKPEALE
                     LLWDMGCRGFQGYLFAKPLLPKEFIAFHDRIVADGMRFKLSETPRA"
     gene            7955..8626
                     /locus_tag="PLG14_06100"
     CDS             7955..8626
                     /locus_tag="PLG14_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HOX48744.1"
                     /translation="MSLREPRTEEARFGVHGGLLKWGSHVCCLYSGAAQRDEILLGFF
                     SEGSRSGDLLAYGSAEGQLRDLPSRLAASAPGPIHLLRRDPRELCCAGGRIPPWIARD
                     RPEGPSQGFLLEEGRRVRSAVDMSWAVEEAIGAGALAEAEVELDQAVRERPWIALCMY
                     DLELFSNSNIVLALRFHPFVVGREGLMPNAFYERLSRLKGDFRAGRAPKEAEARVEGR
                     RSVGA"
     gene            8677..8934
                     /locus_tag="PLG14_06105"
     CDS             8677..8934
                     /locus_tag="PLG14_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48745.1"
                     /translation="METKHLVLLGLAVADIPAYVFLFKLLFKGAGGGKLPRLGEIFAS
                     ENFKDRGGEMKLLLLVTASALLVLAEYLAILSLFPGLAGAK"
BASE COUNT         1267 a         3046 c         3394 g         1342 t
ORIGIN      
        1 cgcggcctcg ggccgcctcg cctccggcgt cgagtagtag gcctcggggc cggagagggg
       61 aagaaggagc cgcccgatct ccgcgccgcg ctttccgagg ttgtactcga ggacgtgggg
      121 caggaagacg ttcatcgcga cgccgtgggg cacatgacag acgccgcccg tcgcgtggcc
      181 caggctgtgc acgagtccga ccatggaatt cgagaaggcg atgcccgcca tcgtggaggc
      241 caccgcgagg tccagcctcc cccgggcgtc gccggggttc ttcgtgacgt gcacgatccg
      301 ctcggagacg aggcggatcg ccgtgtcggc gtagacgtcg gagagcgggt tcttccccag
      361 gcagtaggag gcctccaccg cgtgggtgag ggcgtccatg ccggtcgccg ccgtgacgac
      421 cggcggcagc gtgagggtca tgcgcgggtc caggacggcc gcgtcgggca ggagggaggc
      481 ggacacgaag aggagcttgc ggtcgcgctc gtcgtcgcgc acgacggcga ccagggtgca
      541 ctcgctgccc gtgcccgccg tcgtggggac gacgacgaag ggggagagcc tgcccgctat
      601 cgcgcccgcg cctgagtagg cgacgaggtc gtcgccgccc ttggtgacca ggacgttgac
      661 cgacttcgcg gtgtcgatga ccgagccgcc gccgacggcg acgatgccgt cgcaggcggc
      721 ctcgcggtag gcgcgcgcgg cggccgcgac gacgcgggtc ccggagtcgg gcgggacgtc
      781 gtcgaagacg gccgcgaagg ccaggctcga ctcgccggcc gccctgagga ggacgtcgac
      841 gaggcccgcc tgcctgactc ccttgtccgc gacgaggagg ggccggcggc agcccaggcg
      901 ctcgagctcg ttcggcaggg agtccagggc cttctctccc gcgaggatct tgaccgggtt
      961 gtggaattcg aaatagcgtc cgcgttccat gctcactcct tggtggggcc cgcgaagggc
     1021 agggccaggt agattagggc gcgctcggcg tgcttgcgcg cgaagggcgc gctcggcacg
     1081 cgcttcagga cccgcgccac gacgaagcgc ggcaggatga ggctctccac gaggccgagg
     1141 gccctgatga tcgccatcgt cgagggcagg tctccggaga cggcgagccc gttcctcgcg
     1201 tagacctcga aggcgctcgc ggagaaggtc aggaccgcga gggcggccga caggttctgg
     1261 aagcgcatga gtacgtcggg cgactcgggg ccgcggccca ggtagcggag ccgcccgtcc
     1321 tcccccatgc ggagcgcggc cctgggcccc ttcgggtgga ccgcgagggc gaaggagaag
     1381 cccgggggca ggtcggcgag ctcgcgcctc aagccggggt cgacgcgatg cgcgaccgcg
     1441 aaggcgcggc ccacgaggcc cagggccagg gcggcgtagg ccttggccag gggccgcccg
     1501 ggcggggtga gcgcgggagg cgggagcccc gcggcgggcc gcctcgttcc atcatccatg
     1561 gcatcctcct tgtttccggc tccggggggc gcgacgcgcc tctacggggc cgcttgccgg
     1621 gcgggttccc cgagggcgac cgagaggagg gccgcgacgc ggctctcaga gatcctcgat
     1681 ccccgcccca ggatcctctt ctgcatcacg aggccgtcca ggacggcgac gaccaggctg
     1741 ccgacctcct cgtccttccg cgaggccagg cccagcctgg ccagctcctc gaccaccatg
     1801 tcgatccagc cgcggtagct cgcggccatg cgctccctca cggcctcgtt gccctcgaag
     1861 gcgcactgca ccaaatggac gtgggtggcc gatctcgtgt cgtcggccag gatctcgcgg
     1921 accagggcct ccagcatgag ggggaggccg ctcgcgccct ccgagcgggc cagctcgaag
     1981 atgcggtcgg tgagggcggc catgtggcgc tccgcgatgt ccaggacgag ggcgtccttg
     2041 ctggggtagt ggtagtagag ggtcccgcgc gagacgccgg cggccttcgc gatgtcggcg
     2101 aggctcgccg cctcgacgcc gcgctcggag acgacggcct ccgcggccgc gaggatgagg
     2161 tccctggagt cggcgtcggg attgaccgcg gccatcaggg tcttcccgcg gcttgggtcg
     2221 ccggggctgc cccgtcggcg gccgcgcccg cggcctcggg cccgaccggc gcgccccggt
     2281 ccgccgcggc ggcccgcgag gcgtcccgcg atccagccgg agcgccgatg ccgagccgcg
     2341 cgagggtccg cgccgtcggg acgccgtcgg ggccgtagcc cttcttgcgg tagtaggcct
     2401 tcacgagggg ctcgatgggc acgaccgagt cgaccgggtg ccgcgtcttc ccctccgtga
     2461 ggaagcggcc gggcagggtg tcgtcggccg cggtgacgcc catgaggcag ttcatcctgc
     2521 gctccaggac gtgcgcgcgc cggcccgcca tgcggaagtc ccgcggcgtc acgcgccgcc
     2581 cggtgatggc cccgaccagg cccgagaggc aggaccagtc gagcatcgac tgggcgacct
     2641 ccgggaggag ggtcatcgcc agcttgagga ggggcttggg cgtgtacttg gcgacggggg
     2701 gctcgaggag gtagccgaag gcggagaact ggcaggtctg ggtcgagttg accgccgtga
     2761 agaggtcctc gaagaagtcc acccagctcg ccttggagag ccgcgagtac tgggggatga
     2821 agccgaagat ggcctccagg gcgatgggat aggcgttgag gtggcagccg ccgcggttcg
     2881 cgaccgcgta gttgaggcct tggccccagc cgccccgcgg gtcgtaggcc gccatctcca
     2941 tgcccttcac ctgggccgcg aactcgaggc cgccgtactt ctcggcgagg cgcctcgagc
     3001 cgagggccag ctccgcgccc tcgccgcggc ggtgggcgat gtcgtccagg atgcgcgcga
     3061 tgttgtccgt ccggccgaag gcgagctcgc tcggccgcag gcccttctcc gcggcctcca
     3121 tcgcccagct cgccgtcgcg ccgcaggaga tcgtgtccag cccgagctcg cccatcctgt
     3181 cgttccagac ggcgaccagg tcggggtcga agttcatgat gttgcccccc cagaggccga
     3241 tcgtctcgta ctcggggatg tggcgcttct ggccgtcggg gtaggtcccc ttgtggccgc
     3301 agagcaccgc gcagggcgcg caggccgcgt gctccgtggc gtagcgctcg gccatcgcct
     3361 cgccggagag ggagcggcag cgctcgtcgg tgcggtcgcg gaagttgcgc acgggcgcgt
     3421 agccccggtc gacgccggga ttgacgccgt agttcgtgcc gtaggcgcgg taggagagga
     3481 cgaaggggtt gcggttgatg tgcttccggg ccttggcgtt cagcctggcg tagcgctcgg
     3541 ggtcggcggg ctcgatcctg taggcccttc ccgtggcgac gacggccttc aggttcttgg
     3601 cgcccatcac ggcgcccatg ccgccgcggc ccaggtagcg gttccccgag cgtatgacgg
     3661 cgtagaggac gccgttctcg ccggcggggc cgatgacgag ggcgccctgc ctggggttgt
     3721 ccacgagccg ctcctgggcc ggccccgtct cgagtcccca gagcccttcg gcctcctcga
     3781 agcggacgcc gtcctcgtcg atccgcagga ccagggggga gctcgccctg ccggagacga
     3841 ggatcccgtc ccagcccgcc gtcttgcagg ccaggccgaa gggcccgccg caggaggagg
     3901 agaccatgat gcccgtgagc ggcgacttgg tgacgcccgc gaagcgcgcc gagcagggcg
     3961 cccccgtccc ggcgaaggag cccatcatga aggcgaggat gttctcgggg ccgagggggt
     4021 cgacctcgcg gagggctgtc ccgtctgccc cgcccagcct gtcgtagtag agcttggtgc
     4081 ccaggccctt gccgcccagg tagccgagga ggtcctcctc ggccggcgag aagacgcggt
     4141 aggagcgcga ggagaggtcg atgtcgaggc agcgcccgga ttctccgacg atggtcttca
     4201 tgaggcggcc tcctcggcgc cgcccggggc ggcgatcgtt atgcagtcca tggggcagta
     4261 gaaggcgcag tagccgcagg agacgcaggc ctcgggcgcc gcgaggctcg ggtaggcccc
     4321 gaggccgccg ggccggccct tgcccatcgc gaggcaggag gcgggacact tctgcgcgca
     4381 ggcgccgcag gagatgcagc gcgagagctc gaagacgggc ttggcccaga gggcgcgcgg
     4441 catgaggcgg gcgacggccg cgccgccctc gtccaggacg gcggacttgg ggcagacgcg
     4501 gccgcaggcg ccgcactcga cgcagcgcgg gcgggcgatg gcgtggcgcg agccgcgctc
     4561 gccggagatg gcggcgacgg ggcagacgcg gacgcaggcg gcacagccta tgcaagccgg
     4621 gccgatggag tgggccatgg gcgcctccgg ggtatgcact cagtcggtcg aacgaccgac
     4681 tggccccaga ttaggcccac gcactcccct cgtcaagcgc caatcgagaa atatcagggg
     4741 cgaggtcggt ataacctacc cctcagctcc agtcgaggag acggcgctct ccctgaaggc
     4801 cgcggtaggg cgccgcgtcc atggtcgtct ccaggcagcc gcggtagcgg ccggcttggt
     4861 catagagggc cctgtattcg atgacgacga acttgccgcc catctcgatc cagaagcggg
     4921 cctggctctt ctccttcttc ttgaaggccg agaggatgct ctccacgacg gcgacgctct
     4981 tctgggggtg gcagttctgc accgcccgcc cgatcacggc ggggctgcgg gggaagatcc
     5041 gctccggcgc gtcagaatag tagaggacgc ggtcgttttc gtcgacgacg ctgaagtcca
     5101 gggggagggc gcgcagggcc aggtccagga cctcgaggcc gatccgcccc gtgttgagct
     5161 cgaggaggcc ggcctgcggc gccgcgccgc cgggggcgtg agaggcgccg ccggaagccg
     5221 gagcgccgac aggcgcggcg ccgggcgcgg aggccggccc gccgccggga gccgcggact
     5281 cgccgtcccc ggtccgcgcg gcggccccgg cgcccgagcc cgaggcgagg cgggcgagga
     5341 cgaggtcggc gtcgtaggcc gcgcccggct cgatccacgc gaagccgatg gcctcctcgc
     5401 ccttcctgat ctccgcccac tcgcgctccg agagccgcct cagggcgttg gggtagagga
     5461 tccgctcctc catgaagacc atcctgcgga cggccgcggc cagcttccgc gccgcggagc
     5521 ggaagcgcct cgccccttcg gagcccgagg cctcgcggta ggcggcctcg gcctccttga
     5581 gcaggaccct gatctcgtcg tgcttgcccc acatcacctt ggagggcccc gtgaagccgt
     5641 gccgctcgag gtaggggaag agctggtttt ccttgcgggc gtagtggacc gcgagcttgg
     5701 cgagctcgcc cagggccgcg ccggcctcgg aggagcccga gccgaggccc caggcccagg
     5761 ccctcgcgcc gcggaggagg cgcccgagga gccgctcggc ctcgcggttc tccgccatga
     5821 agctgtggac ggggtggccg ggcatcctgg agggcttgtg ccccttctcc agggaggact
     5881 cgaagaccgc gacgtggacc tcgcagagcc gctggatctc ctccaccggg aagccctcgt
     5941 cgacgagggc ctgctccatc gccgcgactt cctccgccga cacgcccttg atgagggtgt
     6001 cgaagtcgcg cttgacctcg gcgaccgtcg cgccgccatg gagccgcccg atgatctcct
     6061 tgagccgcgc ctgcttcccg ggatccctcg taaggtactc gctcatgccg attcctcctc
     6121 gttcatggct ttcagtatcg cgcgggagag ggaaatttgc cgtaacccag gttacggaaa
     6181 cgcggggctg gcggggagtc ggctggggaa ggggcgtagc ggcgcgggcg gcgggcttca
     6241 ggccctgggc gtctcggaga gcttgaagcg catcccgtcg gcgacgatcc tgtcgtggaa
     6301 ggcgatgaat tccttgggga ggaggggctt cgcgaagagg tagccctgga agccgcggca
     6361 gcccatgtcc cagaggagct ccagggcctc gggcttctcc acgccctcgg cgacgaggga
     6421 gaggtcgagc cccttcgcga tggcgacgag ggagcggacg atgatccggt cctggcggtt
     6481 tccgtccagg ttttcgacga agagccggtc tatcttgatc tcgtccaggg ggaagcggtg
     6541 caggtgggac agcgaggagt agcccgtgcc gaagtcgtcc acggagatgc ggacgccgag
     6601 ggcggcgaag cgctcgaggg tggccgtcgt cgccgtcggg tcctcgaaga ggctcgtctc
     6661 ggtgagctcg acggtgagga ggtcgggctt ggcgcccgtg taggcgagga cggcggcgag
     6721 ggtctccgcg gcgtcctcgc tcttgaactg ccgggcggag aggttgacgg agaggccgat
     6781 ctccgggcgt ccgctcttgg cccaggccgc gaggcggcgg gcgcactggt agaggagcca
     6841 tttcccgagg gggaggatga ggcccgtctc ctcggccagg ggcacgaagg tggagggcat
     6901 gaggatgccg cgctccggat ggcgccagcg gatcagggcc tccgcctcga tccggtcgac
     6961 gaggagggag ccgtcctcca ggccgcgggc gaagagcttg ggctggaaac agaggaagaa
     7021 ctgctcgccg atgcggtcca gggcggtcgc ctctatggcc ttgatgatgg agttctggag
     7081 ctcgaccttc tccacagcct tctcgaggag gggcggccgg aagaggagga aggccttgcg
     7141 cgcctccacg gaggcgccga cggcgatgtc cgcgtggtgg aggaggaccg accgctcctc
     7201 gccgtgctcg ggccagtagg cggcgcccga gtaggcgccg acggccacgt tgaagccggc
     7261 gaagatgtgg ggctcgttga gccggggcag gaggcgtcgg aggaaggctg ccaggtcctc
     7321 gcccttcatg ccggggagga ggaggacgaa ctcgctctcg tgggtatgga agagcctgtc
     7381 ctcctcgcgg aggagggccg tgatccggca gccggtctgg tagaggatcc actcgctcac
     7441 gctcgccttg agcgttttcc tgaccacgcc gtagttctcg cccagctgga cgatgaggac
     7501 ggcgaagccg cggctgggcg actcgcgcct cgcggtgtcg atgaggtcgt cgagctcgag
     7561 ctcgagccgg aagtgggtcg ggagcccggt ctggggctgg agggatagtt tgcgctcgag
     7621 ctcggccacg cgctggccca gggcctcgtt ctcgcggcgc aggcgctcgt tctcgtcgga
     7681 gatgaggctc gtgtcgggct taggcccctg ttccgccctt gcgtccactt tcgatgtgct
     7741 ccatgagaat agcgacgtag tgccccaaag ccgcgagccg ggctccatag tacttatcgg
     7801 caggttcttg cgcaacgagg ggcaaaaagc cgcctcttcg atccgccttc cggaccgcct
     7861 tcagcccttg caggcccgcc tgccggcgac gatactgaaa gtctatcgca ttcttcggct
     7921 ctccgccaaa gtcctgcagg cggaaggggg cagcttgtcg cttcgcgaac ccaggacgga
     7981 ggaagcgcgc ttcggcgtcc acggcggcct tctcaagtgg ggctcccatg tctgctgcct
     8041 ctattcgggg gcggcccagc gcgacgagat cctcctcggc ttcttctccg agggcagccg
     8101 gagcggggac ctcctggcct acggctccgc ggagggccag ctccgggacc tgccctcccg
     8161 gctcgccgcc tcggccccgg gcccgatcca cctcctcagg cgcgatccgc gggagctctg
     8221 ctgcgccggg gggcggattc ccccctggat agcccgcgac aggccggaag ggccttccca
     8281 gggcttcctc ctggaggagg ggcggcgcgt gcgctccgcc gtcgacatga gctgggccgt
     8341 cgaggaggcc atcggagcgg gcgccctggc cgaggccgag gtcgagctcg accaggcggt
     8401 cagggagcgc ccctggatag ccctgtgcat gtacgacctc gagctcttct cgaactcgaa
     8461 cattgtcctt gccctgaggt tccatccctt cgtggtaggc cgcgagggcc ttatgcccaa
     8521 cgccttctac gagcggctct cgcggctcaa gggggatttc cgcgccggcc gggcccccaa
     8581 ggaggccgaa gcccgggtgg aggggcgcag gagcgtcggc gcctagtctt tgcggggatt
     8641 gacccttacg ccttcccccg cgcatcctca ggatccatgg aaaccaagca cctcgtcctc
     8701 ctcggcctcg ccgtcgccga tatacccgcc tatgtcttcc tcttcaaact cctcttcaag
     8761 ggcgcgggag gcgggaagct tccccgcctg ggcgagatct tcgcctcgga gaacttcaag
     8821 gacaggggag gggagatgaa gctcctcctc ctcgtcacgg cctcggccct cctcgtcctg
     8881 gcagagtact tggccatcct gtccctcttt cccgggctgg ccggggccaa gtaggcccgg
     8941 cgccgtgccc aggcgcaact tcctcctcgt cctcgcctac gacgggaccg acttctcggg
     9001 ctggcagcgc cttcccggga aggcccggag cgtccaggcc gaggtcgag
//
LOCUS       DAOOSJ010000095         8953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4943_length_8953_cov_5.933356, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000095 DAOOSJ010000000
VERSION     DAOOSJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_8953_cov_5.933356"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLG14_06110"
     CDS             <1..1362
                     /locus_tag="PLG14_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOX48746.1"
                     /translation="RLSEEGKDVALIERDPDVARVAANALDCLVVQGDGSLPEILERA
                     GTSKATHFIALTGSDELNIVTCSVVAAEHPGTARIARVRNSYFTKLEPSRRSFMGVDR
                     FVNPDIETARAFIALVARGPGESLVRFEDEDLVLRSTRLAPGSSLAGRSLRESRAALG
                     RNFLAAALEREDGVEVPSGETSPAAGETLYLLGAPADLDAMLGEAPQAEERCSKIVIA
                     GGGSIGRYIAEGFLGTSEGSELGLSRGRGRIFGRKGRRNIVIVERSMETCKLLAREVP
                     GALVLNRELGDEELFLEEGLGDADLFLAVTQDQELNLLTAARAKDAGIRRCLALSENN
                     CYSELAARLGVDGVISVKSNVVSSIVEYLRGGALRTLHSFYDRGIKILEFLVPEGSEL
                     HGAAIRDLKLPRGALVIYVNRLGRSALPAGDTRLASGDRLGIFTSMEAIRGVEELFLG
                     EGR"
     gene            1359..2816
                     /locus_tag="PLG14_06115"
     CDS             1359..2816
                     /locus_tag="PLG14_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOX48747.1"
                     /translation="MKTRAILKLLSLLLLVLAGFMLVCSAAALRFHDGSEVLRAFLIP
                     AAGCFLVFGLVFGLTPGKERPYFSAKSGYLFVSLAWVAASAVGALPFVLSGAIPSYTD
                     AFFETMSGFTTTGASVLADVEAAPRAILLWRSTTHWLGGMGIVVLTVAIFPLLGLGGL
                     QLVEAEAPGPSVDKITPRMAGTAKILWLIYVLLTAAEILLLLLGGMGLFDAVNHTFAT
                     VATGGFSTKNLSVGHFASPYIDIVVTVFMVLAGMNFALFYKAGTGKLREALRDTELRA
                     YLGVFLAASLLIALDLSKNGVFPAFGTAFRHGAFQSASILTTTGFATANFDLWPPFSR
                     VVLLLLMFIGGSAGSTAGGIKVVRYLVLMKQSAVELKSLVFPRGVFSVRLNGEPVRKM
                     LVFDTASFFFMYFVTILACTLVVASAGQGVETALTATLATLGNIGPGLALVGPVANYG
                     FLPDYVTWTLSFAMLVGRLELYTVFVLFSSAYRRS"
     assembly_gap    2854..2953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2984..3934)
                     /locus_tag="PLG14_06120"
     CDS             complement(2984..3934)
                     /locus_tag="PLG14_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48748.1"
                     /translation="MNAANFLANILVLGVPFSVALLLAGLGEMFNQRAGVFNLGCEGI
                     MAMGAFLAFLPPYFLGAAMPMGAANLLGIAVAMGAGALLGLLFALVTVTFRAPQGIAG
                     IGLQMLGWGTAGTLFRHYVGGVTGVQGIKSLPIPGLSKIPLLGPILFDHNPLVYLAFL
                     LVPASWYLLFKTPWGLKVRSVGTYPRAADTLGINVRRVRYQALALGGAMAGLAGAYLS
                     LAQAKMFADDLIAGRGFIAVALVYFGRWSPVGIMGGALLFSLAQSFQLSVQVAGIKFP
                     YEFAVMLPYVLVIVVLSLAKKARGAEPSDLGKPFDREQRV"
     gene            complement(3931..5157)
                     /locus_tag="PLG14_06125"
     CDS             complement(3931..5157)
                     /locus_tag="PLG14_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48749.1"
                     /translation="MNKAFKLAYGAMVALAAAAAAMLVGALVMAAIGADVAKTYATIL
                     TEPLKDLFGLTEIVVRAIPLSLIALGVAVAFRSGILNIGAEGQMLMGILASSATAIAL
                     PELPKPLLLPLALLAGAAGGALWGSLAGLLKARLGVNEILSTVMLNYVAAQFYTFLLR
                     GPMMDPQELLTGTGTPQTVRLPKAAWLDRLVPGTRLHYGLVIALVLAVAVFLLLWRTT
                     LGFRMRAAGAEAKAARYAGVSVERYLVVAMLLSGAFAGLAGAVEVLGVHRRAIEGISG
                     GYGFTGIVVALFGGLHPAGIVPASAFFGLLLLGADMTQRSASVPANMVLVLQGAIILS
                     IVSARQFMNNPYVQDKATRRLAKLFGKAPPLSPAVAEAACPDPSDVAEPCAAGEPGAA
                     GASAGAAPGAGKGGRA"
     gene            complement(5150..6673)
                     /locus_tag="PLG14_06130"
     CDS             complement(5150..6673)
                     /locus_tag="PLG14_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48750.1"
                     /translation="MRPEPIRTLELRGITKRFPGVLACDHIDLSVPEHSVLALVGENG
                     AGKTTLMNILMGLYQPDEGSILLNGREARFRSPNDAFGHGIGMVHQHFMLVPNMTVAE
                     NVALGIKEAHRTLSLDMKEVRRRIAEVSERHELPVQPDAYVWQLSVGEQQRVELVKTL
                     CLGARFLILDEPTSALTPQETDELIALLKKMSAELSIIFISHKLNEVKALSDTVSILR
                     RGRVVFRGNTADHSSSDLAALMTGHEVNLPRNECAEGCGEARLQVKDLKVRSDRGFLA
                     LDGLSLEVRAGEILGVAGVSGNGQRELADALAGLRRAESGSILLDGQELLNRRPAEVI
                     GSGMGYIPEDRHTEGIVPSFSVKENLVLKDFATRRFSALSFLRLRAIQKNADELRGRF
                     DIRCPSTETQAGSLSGGNIQKVILARELARGPKALVAVYPTRGLDMGAEEFIHKQLLA
                     RRKEGAAVLLISEELDEIMNLSDRIAVIYKGRVLKVLPGGTATKQSLGLLMAGVVDE"
     gene            complement(6758..7753)
                     /locus_tag="PLG14_06135"
     CDS             complement(6758..7753)
                     /locus_tag="PLG14_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOX48751.1"
                     /translation="MKRLMAILMALAVVAGAAYGAPKSKVRIALIIESTVDDKGWGQA
                     MHDAVMAVQKKYGAALVEYNYSEKMKPVDAGSAARQYASRGYDVIIAHGAQFKNLVLE
                     LAEEFPKTTFAFGTSAEIGPKNVFTYMPQSEETGYVNGVIAGLVTKTNVIGVVGPVDA
                     GDSARYIRGFILGAKASNPKAEVKVAFTGSFADFVKAAELAHTQVKAGADVLTGAAQQ
                     ATGALRAVAEYKDKPIWWLGQDTLQIGMPEGFKVVAAASYDYAPVIEALIDKRQAGVL
                     GGENVPMTYGNLGFVFKYNEKVGAVLTAAVRKAADQARADVTAGKLKLDWAAVKF"
     gene            8026..8514
                     /locus_tag="PLG14_06140"
     CDS             8026..8514
                     /locus_tag="PLG14_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOX48752.1"
                     /translation="MTERDKELLSLAVERAERSRREGNHPFGAVLADGKGELLLEAGN
                     TVPEEGDCAGHAETNLARLASRTYPRDFLRNCSLYTSAEPCAMCAGAIYWSGIGRVVY
                     ALSEAELKALTGDDPENPTLDLPCREVFARGQRPVAVEGPFALASARRVHEGFWKPAG
                     RG"
     gene            8695..>8953
                     /locus_tag="PLG14_06145"
     CDS             8695..>8953
                     /locus_tag="PLG14_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48753.1"
                     /translation="MSFEPKLDIDPVLEPRKARAALVWGLGLPSCEPTKEAEPWLAEI
                     LGRARAAGEAFLPPARKAAVRDMLRHGAYKPAGRAKPSSEYL"
BASE COUNT         1258 a         3063 c         3202 g         1330 t
ORIGIN      
        1 cgcctctcgg aagagggcaa ggacgtggcc ctcatcgagc gggatcccga tgtcgcccgc
       61 gtcgcggcca acgccctgga ctgccttgtc gtccagggcg acggcagcct gccggagatc
      121 ctggagaggg caggcacctc caaggcgacc cacttcatcg ccctgaccgg ctccgacgag
      181 ctcaatatcg tcacctgctc cgtcgtcgcg gccgaacacc cggggacggc caggatagcc
      241 cgggtgcgca actcctattt caccaagctc gagccctcgc ggcgctcctt catgggcgtg
      301 gaccgcttcg tcaatccgga catcgagacg gcgcgcgcct tcatcgccct ggtggcgcgg
      361 ggcccgggcg agagcctcgt gcgcttcgag gacgaggacc tcgtcctccg ctccacgcgc
      421 ctggccccgg gctcctccct cgcgggacgg agcctccgcg aaagccgggc ggccctggga
      481 aggaacttcc tggccgcggc cctggagcgg gaggacggcg tggaggtccc ctccggcgag
      541 acctcgccgg ccgcggggga gaccctctac ctcctgggcg cgccggctga cctggacgcc
      601 atgctgggcg aagcgccgca ggcggaggag cgttgcagca agatcgtcat cgcggggggc
      661 ggctccatag gccgctacat cgcggaaggc ttcctgggca cttccgaggg ctccgagctg
      721 ggcctctccc gcggccgcgg ccggatcttc ggccgcaagg gcaggcggaa catcgtgatc
      781 gtcgagcgct cgatggagac ctgcaagctc ctggcccggg aggtgccggg cgcgctcgtc
      841 ctgaaccgcg agctgggcga cgaggagctc ttcctggagg agggcctggg ggacgccgac
      901 ctcttcctgg ccgtgaccca ggaccaggag ctcaacctcc tcaccgccgc cagggccaag
      961 gacgcgggga tccggcgctg cctggccctc tcggagaaca actgctacag cgagctcgcc
     1021 gcccggctgg gcgtggacgg cgtgatctcc gtgaagtcga acgtcgtctc ctctatcgtc
     1081 gagtacctgc gcggaggggc cctgaggacc ctccactcct tctacgaccg gggcatcaag
     1141 atcctcgagt tcctggtgcc cgagggctcg gagctccacg gcgccgcgat cagggacctg
     1201 aagctgccgc ggggagccct cgtcatctac gtgaaccggc tgggaaggag cgcccttccg
     1261 gcgggcgaca ccaggctcgc gtccggggac cggctcggca ttttcacgag catggaagcc
     1321 atccgcggcg tcgaggagct cttcctgggc gagggtcgat gaagaccagg gccatcctca
     1381 agctcctctc cctcctcctc ctcgtcctgg cgggcttcat gctcgtctgc tcggccgcgg
     1441 ccctgcgttt ccatgatgga agcgaggtcc tccgcgcctt cctgatcccg gccgccggct
     1501 gcttcctcgt cttcggcctg gtcttcggcc tcaccccggg caaggagcgc ccctacttct
     1561 ccgcgaagtc gggctacctc ttcgtgagcc tggcctgggt cgccgcgagc gcggtcggcg
     1621 ccctgccctt cgtcctctcg ggggccatcc cctcctacac ggacgccttc ttcgagacga
     1681 tgtccggctt caccacgacg ggggcttcgg tcctcgccga cgtcgaggcc gcgccccgcg
     1741 ccatcctcct ctggcggtcg accacgcact ggctcggcgg catgggcatc gtcgtcctca
     1801 cggtcgcgat cttccccctc ctgggtcttg gcggcctcca gctcgtggag gcggaagcgc
     1861 ccggccccag cgtcgacaag atcacgccgc gcatggcggg cacggcgaag atcctctggc
     1921 tgatctacgt cctcctcacc gcggcggaga tcctcctcct cctgctcggc gggatgggcc
     1981 tcttcgacgc ggtgaaccac accttcgcga ccgtcgcgac gggaggcttc tcgacgaaga
     2041 acctctcggt cggccacttc gcgagcccct acatcgacat cgtggtgacg gtcttcatgg
     2101 tcctggccgg gatgaacttc gcgctcttct acaaggcggg cacgggcaag ctgcgcgagg
     2161 cactgcgcga cacggagctc agggcctacc tcggcgtctt cctcgccgcc tccctcctca
     2221 tcgccctgga cctctccaag aacggcgtct tccccgcctt cgggacggcc ttccgccacg
     2281 gcgccttcca gtcggcctcc atcctcacga cgacgggctt cgcgacggcg aacttcgacc
     2341 tctggccgcc cttctcccgc gtggtgctgc tgctcttgat gttcatcggg gggagcgccg
     2401 gctcgacggc cggcggcatc aaggtcgtgc gctacctcgt cctcatgaag cagtcggcgg
     2461 tcgagctcaa gtccctggtc ttcccgcgcg gcgtcttctc ggtgcgcctg aacggcgagc
     2521 cggtgcgcaa gatgctcgtc ttcgacacgg cctcgttctt cttcatgtac ttcgtcacga
     2581 tcctggcctg caccctcgtc gtcgcctccg cggggcaggg cgtggagacg gcgctgacgg
     2641 cgaccctcgc gaccctgggc aacatcggcc ccggcctggc cctggtgggg cccgtcgcca
     2701 actacggctt cctgcccgac tacgtcacct ggaccctgag cttcgcgatg ctcgtgggca
     2761 ggctggagct ctacaccgtc ttcgtcctct tctcctcggc ctaccggcgc tcctgagcct
     2821 cggcgccgcc ggcgcgggcc gcccgcgggc ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnngcgcgtc cccgcgcggc ccggccgcct cgcctagacc cgctgctccc
     3001 ggtcgaaggg cttcccgagg tcggagggct cggcgccgcg ggccttcttg gccagggaga
     3061 ggacgacgat gacgaggacg tagggcagca tcaccgcgaa ctcgtagggg aacttgatgc
     3121 ccgccacctg gacggagagc tggaaggact gggccaggct gaagagcaag gctcctccca
     3181 tgatgccgac cgggctccag cggccgaagt agacgagggc gacggcgatg aagccgcggc
     3241 ccgcgatgag gtcgtcggcg aacatcttgg cctgggccag gctcaggtag gcgccggcca
     3301 ggccggccat cgcgccgccc agggccaggg cctggtagcg cacgcggcgg acgttgatgc
     3361 ccagggtgtc ggcggcccgc gggtaggtgc cgaccgacct caccttgagg ccccagggcg
     3421 tcttgaagag caggtaccag gaggccggca ccaggaggaa ggcgaggtag accagggggt
     3481 tgtggtcgaa gaggatgggc ccgaggaggg ggatcttgga gaggcccggg atgggcaggc
     3541 tcttgatgcc ctggacgccc gtgacgccgc ccacgtagtg gcggaagagg gtgccggccg
     3601 tgccccagcc gagcatctgg aggccgatgc ccgcgatgcc ctggggcgcc ctgaaggtga
     3661 cggtgacgag ggcgaagagg aggcccagga gggctcccgc ccccatcgcg accgcgatcc
     3721 cgaggagatt ggccgccccc atcggcatgg ccgcgcccag gaagtagggc ggcaggaagg
     3781 cgaggaaggc gcccatcgcc atgatgccct cgcagcccag gttgaagacg ccggcccgct
     3841 ggttgaacat ctcgcccagc cccgcgagga ggagggccac ggagaagggc acgcccagga
     3901 cgaggatgtt ggcgaggaag ttggcggcgt tcatgcccgg cctccctttc ccgcgccggg
     3961 cgcggcgccc gcggaagccc ccgcggcgcc cggctccccg gccgcgcagg gctcggcgac
     4021 gtccgacggg tcggggcagg ccgcctccgc gacggccggc gaaaggggcg gcgccttgcc
     4081 gaagagcttg gcgaggcgcc gggtcgcctt gtcctggacg tagggattgt tcatgaactg
     4141 cctcgcggag acgatggaga ggatgatggc gccctggagg acgaggacca tgttcgccgg
     4201 cacgctcgcc gagcgctggg tcatgtcggc cccgaggagg aggaggccga agaaggcgga
     4261 ggcgggcacg atgcccgcgg gatggaggcc gccgaagagg gccacgacga tgcccgtgaa
     4321 gccgtagccc ccggagatgc cctctatcgc gcggcggtgg acgcccagga cctccacggc
     4381 gcccgcgagg cccgcgaagg ccccggagag gagcatcgcg accacgaggt agcgctccac
     4441 ggagacgccc gcgtagcgcg cggccttggc ctcggcgccc gccgcgcgca tccggaagcc
     4501 cagggtcgtc ctccagagga ggaggaagac cgcgacggcc aggaccaggg cgatgacgag
     4561 gccgtagtgg agccgcgtcc cggggaccag gcggtccagc caggccgcct tgggcaggcg
     4621 cacggtctgg ggcgtgcccg tgcccgtgag gagctcctgg gggtccatca tcggcccgcg
     4681 gaggaggaag gtgtagaact gcgcggcgac gtagttgagc atcaccgtgg agaggatctc
     4741 gttgaccccg agcctcgcct tgaggaggcc cgcgaggctc ccccagagcg cgccgcccgc
     4801 ggcccccgcg aggagggcga gggggaggag gaggggcttc ggcagctccg gcagggcgat
     4861 cgccgtcgcg gaggaggcga ggatgcccat gagcatctga ccctcggcgc cgatgttgag
     4921 gatcccgctc cggaaggcca ccgcgacgcc cagcgcgatg agcgacagcg ggatggcgcg
     4981 gaccacgatc tccgtcaggc cgaagaggtc cttgagcggc tccgtgagga tggtcgcgta
     5041 ggtcttggcg acgtcggccc cgatggcggc catcacgagg gcccccacga gcatggccgc
     5101 ggcggcggcg gccagggcga ccatggcccc gtaggccagc ttgaatgcct tattcatcga
     5161 cgacccccgc catgagcaat cccaggctct gcttcgtggc cgtgcctccc ggcaggacct
     5221 tgaggacgcg gcccttgtag atcaccgcta tcctgtccga gaggttcatg atctcgtcga
     5281 gctcctcgga gatgaggagg acggccgcgc cctccttccg cctcgccagg agctgcttgt
     5341 ggatgaactc ctcggcgccc atgtccaggc cccgcgtcgg gtagaccgcg accagggcct
     5401 tgggcccccg ggccagctcg cgggccagga tcaccttctg gatgttgccg ccggagaggg
     5461 agcccgcctg ggtctccgtg gagggacagc ggatgtcgaa gcggcccctg agctcgtcgg
     5521 cgttcttctg gatggcgcgg agccggagga aggagagggc ggagaagcgc ctcgtcgcga
     5581 agtccttgag gaccaggttc tccttgacgg agaagctcgg cacgatgcct tcggtgtgcc
     5641 ggtcctcggg gatgtagccc atgccgctcc cgatcacctc cgccgggcgg cggttcagga
     5701 gctcctggcc gtccaggagg atggagccgg actcggcgcg gcgaaggccg gcgagggcgt
     5761 cggcgagctc gcgctggccg ttgccggaga cgcccgcgac gcccaggatc tcgccagcgc
     5821 gcacctccag ggagaggccg tccagcgcga ggaagccgcg gtcgctgcgc accttgaggt
     5881 ccttcacctg gaggcgggcc tcgccgcagc cctccgcgca ctcgttgcgc gggaggttca
     5941 cctcgtggcc cgtcatcagg gccgcgaggt cggaggagga gtggtcggcc gtgttcccgc
     6001 ggaagacgac cctgccccgc ctgaggatgg agaccgtgtc ggagagggcc ttcacctcgt
     6061 tgagcttgtg gctgatgaag atgatggaga gctccgcgct catcttcttg aggagggcga
     6121 tgagctcgtc cgtctcctgg ggcgtgaggg ccgaggtggg ctcgtccagg atgaggaagc
     6181 gggcgcccag gcagagggtc ttcacgagct cgacccgctg ctgctcgccc acggagagct
     6241 gccagacgta ggcgtcgggc tggacgggga gctcgtggcg ctcggagacc tcggcgatgc
     6301 gccgcctgac ctccttcatg tccaggctga gcgtccggtg cgcctccttg atccccaggg
     6361 cgacgttctc cgcgacggtc atgttgggca cgagcatgaa gtgctggtgg accatgccga
     6421 tgccgtggcc gaaggcgtcg ttgggcgagc ggaagcgcgc ctccctgccg ttgaggagga
     6481 tcgagccctc gtccggctgg taaaggccca tgaggatgtt catgagggtc gtcttgccgg
     6541 cgccgttctc gcccacgagg gccaggaccg agtgctcggg gaccgagagg tctatgtggt
     6601 cgcaggcgag cacgccgggg aagcgcttgg tgatgccgcg cagttccagg gtgcggatgg
     6661 gctccggtcg catcttcagc tcctctcggt aggggatcgg ccggccccgc ccgcgcggcg
     6721 atcgcgctgg cggagccggc cgaggttcgg gcgggtccta gaacttgacc gcggcccagt
     6781 cgagcttgag cttgccggcg gtgacgtcgg cgcgggcctg gtcggcggcc ttcctcacgg
     6841 cggcggtgag cacggccccg accttctcgt tgtacttgaa cacgaagccc aggttgccgt
     6901 aggtcatggg gacgttctcg ccgcccagga cgccggcctg gcgcttgtcg atgagggcct
     6961 cgatgacggg cgcgtagtcg tagctcgcgg ccgcgacgac cttgaagccc tcgggcatcc
     7021 cgatctgcag ggtgtcctgg ccgagccacc agatcggctt gtccttgtac tcggcgacgg
     7081 cgcggagggc gcccgtggcc tgctgggcgg cgcccgtgag gacgtcggcg ccggccttga
     7141 cctgggtgtg ggcgagctcg gcggccttca cgaagtccgc gaaggagccg gtgaaggcga
     7201 ccttgacctc ggccttggga ttggaggcct tggcgcccag gatgaagccg cggatgtagc
     7261 gggcggagtc gcccgcgtcg acggggccga cgacgccgat cacgttggtc ttggtgacca
     7321 ggccggcgat gacgccgttg acgtagccgg tctcctcgct ctggggcatg taggtgaaga
     7381 cgttcttggg cccgatctcg gcgctggtgc cgaaggcgaa ggtggtcttc gggaactcct
     7441 cggcgagctc caggacgagg ttcttgaact gggcgccgtg ggcgatgatg acgtcgtagc
     7501 cgcgggaggc gtactggcgg gcggcggagc cggcgtccac gggcttcatc ttctcgctgt
     7561 agttgtactc gaccagggcc gcgccgtact tcttctgcac cgccatgacg gcgtcgtgca
     7621 tcgcctgtcc ccagcccttg tcgtccaccg tggactcgat gatgagggcg atgcggacct
     7681 tggacttggg cgcgccgtag gccgcgccgg cgacgaccgc gagagccatg agaatagcca
     7741 tgagcctctt catgtttcct cctagggaac tccagggcgg gggacgtgcc cccttggaag
     7801 cccgatcctc gcgcgacgcg cctgccccgt ccgggaagcc cggggcgatc gggccttcgt
     7861 gccagcggcg atgatagcac gacaatcggg gagccgctga aatttctcgc tcttgacgcg
     7921 gcgccggccc tgccccagac tcggggccgc ggccggcgga gcccgccccg gccccgggac
     7981 cgcggtccgg gcgccgcgag aacgcgcggg gccgaggagg aaggcatgac ggaacgggac
     8041 aaggagctcc tttccctggc ggtggagagg gccgagcgct cgcggcggga gggaaaccac
     8101 cccttcggcg ccgtcctcgc cgacgggaag ggcgaactcc tccttgaggc cggcaacacc
     8161 gtgcccgagg agggcgactg cgcgggccac gcggagacga acctggcccg cctcgcctcc
     8221 aggacctacc cgcgggactt cctcaggaac tgcagcctct acacgagcgc ggagccctgc
     8281 gcgatgtgcg ccggggcgat ctactggtcg ggcatcggcc gcgtcgtcta cgccctctcc
     8341 gaggccgagc tcaaggccct cacgggcgac gacccggaga atccgaccct ggacctgccc
     8401 tgccgcgagg tcttcgcccg gggccagcgc cccgtcgccg tggagggccc cttcgccctc
     8461 gcctcggcgc ggagggtcca cgagggcttc tggaagccgg cgggccgggg ctgaaggcgg
     8521 agagccccgt cgcctgcgaa gacgaggagg gcgaggaggc cgctgtcgcg cagaccgggc
     8581 aaggcgcgac ggccgcggat tgaacgaagc gcccgctggt cggccgcgcg gccgcggccc
     8641 cggccgcagc gcgcgccgct cgacccgcgt ccttccttgc tctatgatcg cgccatgagc
     8701 ttcgaaccca agctggacat cgatcccgtc ctggagcctc gcaaggcccg cgccgccctg
     8761 gtctggggcc ttggcctccc ttcctgcgag ccgacgaagg aggccgagcc ctggctcgcc
     8821 gagatcctcg ggcgggcccg cgccgccggc gaggccttcc ttccgcccgc gcggaaggcg
     8881 gccgtccgcg acatgctccg ccacggcgcc tacaagcccg cgggcagggc caagccctcg
     8941 agcgagtacc tcc
//
LOCUS       DAOOSJ010000096         8946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4948_length_8946_cov_5.459341, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000096 DAOOSJ010000000
VERSION     DAOOSJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_8946_cov_5.459341"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLG14_06150"
     CDS             <1..304
                     /locus_tag="PLG14_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOX48754.1"
                     /translation="KKPRYFCEHCGAEVRRDARVCPRCGRFFSSVRCPKCGHTGEAGE
                     FRSGCPLCGYAEAANGSPDPFLSAPAAAEPLPAWIYLGALAALVAAIALLLRALRS"
     gene            complement(324..2447)
                     /locus_tag="PLG14_06155"
     CDS             complement(324..2447)
                     /locus_tag="PLG14_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48755.1"
                     /translation="MRRRTLRGYTLAVEAATFAAALAALLAALFAASVRVDAVYAARR
                     SEEALKATVLVDSVIERGRGLVRRLGAEPGLLSAVALVDDFSDLYLVGEDGRVSAILA
                     KSPGSGVFEGFDMGYGAPGELLSRTVERYIAGSGSSGFGVAVSGVARGVEDERPSVYL
                     ATRLASGWLLGRIRVEAILGELRRIADYSGTVILLASADGRILASTRPGLGLDLLPAR
                     LDLPFEAFGEGWLASEALHEALGDRIVVLSPARERAAPLALLWSLGPAAALAFLAAAA
                     ARSFALSRRALAPLARFAEALEVWDLEPGPPPVEERLLAFEEIASLSRSFRKKREEIG
                     RALEETRRRDERILALNSELEDRVRERTASLEAANERLSGANRELRATQDRLVDAEKL
                     AALGRLAAETAHELNTPLGAIRSSAVHVAEVLEQELARLPGFAAELSDRDLGLYRGLV
                     GRALSAADPARHEERRRSLALVRGLLEERGAADADLLADDLADMGFGEGDEAALSALL
                     ESPRAAELVESAYALSGALRASRLILLSADKAAWTVDALRSFGYGTAADEAQRVDPIA
                     GIETVLALMHGKLKRGVEVLRRYDRDVRVKGRPKQLDQVWINLIGNAWDAMEGKGRLE
                     ISVLREGGEVVVEFADSGSGIPDAVKDRIFTPFFTTKEEGSGLGLGLDICRRIVESHG
                     GSIGFESRPGRTVFRVRLPSYDASG"
     gene            complement(2444..3598)
                     /locus_tag="PLG14_06160"
     CDS             complement(2444..3598)
                     /locus_tag="PLG14_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX48756.1"
                     /translation="MKRQRSWSKARAAAPAALFLAALALASCGRPVLKVALLTKLEAG
                     SVVGASEVNAATLFLEDRAARRNGAGAGPRDGSAAGRPARDIEIVPFDDGWDPDKAVA
                     AYGKARAEGFSFFVTSHTSTCAVRLAEPMRQDGVLAMVTGSTTTLLSGKDDLILRDVP
                     DLRFEQEAIARYVAGLPGRDVLVILDLDNEAYTLPALEAFKAALGSGKRVAEERIRMS
                     ALDIERIRSVVSSTPYDTLYVLVGAYQATAGSFAQLSMAARPAARVVFTPWLNSPALA
                     ATAGPALAVSTLPSHYPPKGSSPEVDAYIARFKARFGYAPTLISLNVYKALEVLAAAW
                     DSGAATPEACKLWIVGKGRVPTSLGEALFDAAGDSSSPFYMIENPVAELR"
     gene            complement(3629..4162)
                     /locus_tag="PLG14_06165"
     CDS             complement(3629..4162)
                     /locus_tag="PLG14_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48757.1"
                     /translation="MKYLLDEEARFWRIIKLRPFRKTEGVSFDIVPLEFLPRIDGVDR
                     VIHQTKALSPGPVGGVERPWYLHPHQEDNLLVLHGTRTVDIYSLEYRRMETFVVEPER
                     VLRGGKVVFEGAAMLVWPCNVFHRIVSGAEGSASLNFAVRHPGFDIDTNFSIYDLDTS
                     TGEHRVVREGRLDQFPG"
     gene            complement(4255..5103)
                     /locus_tag="PLG14_06170"
     CDS             complement(4255..5103)
                     /locus_tag="PLG14_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48758.1"
                     /translation="MIFVDRPDGRRLWRLKPLSAIMPYIMRGRNESAVYFSKDLDVEN
                     AIAYVHRKNAESGQTRYSLFGLVLAAALRTIAEKPELNRFVHRRAIYERNHIAFSFIV
                     KKRLTEEAPEANVKLFLDPKDTIAEATDKFNAAVAAAKEEGKGADEREIDLVGRLPGG
                     KAVITSLFRLLDRFNAAPASMIRGDPLFASAYFANLGSLGLDAPFHHAYEWGTASLFV
                     VLGRMFQKEAPGGAGGSAKRHFINVKITADERISEGIYFAHAASLFQRLVLHPELLES
                     PPGNAG"
     gene            5239..7536
                     /locus_tag="PLG14_06175"
     CDS             5239..7536
                     /locus_tag="PLG14_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48759.1"
                     /translation="MKIRTRMFLSVAAIQGLLFIALFSILLVLSTGTARKALVEAAAG
                     GAKARATRVALTLSRGIVSCGSLVSDLGALRSSGRLDRGLPVLLFESALRREPSVFAY
                     WAIYDKGAWDGRDAALADDPVFAPTGAYVPWAYREEGGIKSQAGMEGDEDVEGYYGEF
                     YTVPFASGRPYLSEPYAEEVEEGKFVLMSTYAEPLFDAAGRKLGVAGCDLSLDSLTAL
                     VQASASGTEAASGKAGAGLAYSALLSPEGFVLAHSSDPELVGKSFFEATAEAGGAAGE
                     AGSAGAKDAGSAAGALAEDAAAVMKAIASGSEELWRRKVPGSKASLLFVAEPVVLSAG
                     LPPWILLECVPENLVLEEGERMRAVMAIFLALGLVTMGFGTFAIATRISRPIVRLTEA
                     FSRMEEGDLTLRAPVASRDEVGNLAKGFNLFAVRLSALMGSFRRSAEELDAAGRRVAE
                     AADRTRDSLGAIRGGMDRAQAEVHTQVVSVAQTTKAAEGIASRIGDLRAAVDRQNDAL
                     AAAAALVERTIAQLGEIARKSESINDEMGALEGAGGEGKARLEAVKTAVREAAERSAD
                     LLAANKVVADVAARTNLLAMNAAIEAAHAGEAGKGFAVVAAEIRGLAENTHGQSKSIA
                     SRVAEIRAAVARVSDSATEAGASFDEIIGRIRQVAGLEGQAREALFEERDGGGRVLEA
                     IAETREAAGLVEAASAEMGRSGEEVRLAMARLAEASEGVAASARVVSGRVAEIEASAD
                     EERSIASENLRIAAGFREEVSRFKI"
     assembly_gap    7623..7722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7754..8341
                     /locus_tag="PLG14_06180"
     CDS             7754..8341
                     /locus_tag="PLG14_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOX48760.1"
                     /translation="MAYDCVMPAAGASSRMGAWKPLLPWRGASLVEACVGAALGACSR
                     VLLVGGERLAELASLFRGEPRVLVVANPAWELGMLGSLQRALPLVEGGAFFCANADMP
                     LIESADYEALKARYAILEASGAPAAVFAAHGGEAGHPVLVPSSYIAEILLLPPGGRMK
                     SFLSGKPSCLVERGPGVLADLDTPDDYERAARLAP"
     gene            8331..>8946
                     /locus_tag="PLG14_06185"
     CDS             8331..>8946
                     /locus_tag="PLG14_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48761.1"
                     /translation="MRPRLGSGRRRRGPPGLAFLPARLGAGQSLSAARASPLLGAALV
                     VLASGFLYVSLFLAVARIGAADEALELRLDRALYSLLGGAAGERAPVGEDLDYVYQLL
                     PRLGGVDSKVAISEAAGRLAESIDAYREARAAADGAAAAGAAADGPAADGTGGAAGGE
                     GEAEAAERARTELAASIESFLRVLHARGSEVRASFNYLLLASGVG"
BASE COUNT         1184 a         3186 c         3217 g         1259 t
ORIGIN      
        1 gaagaagccc cgctacttct gcgagcactg cggcgccgag gtgcgccgcg acgcgagggt
       61 ctgcccccgt tgcggacgct tcttctcctc ggtccgctgc cccaagtgcg gccacacggg
      121 cgaagccggg gagttccggt ccggttgccc cctctgcggc tacgccgagg ccgccaacgg
      181 ctcgcccgac cccttcctct ccgcccccgc cgccgcggag ccccttcccg cctggatcta
      241 cctcggggcc ctggccgccc tggtcgccgc gatagccctc ctcctccgcg ccctccgttc
      301 ctagctagag tcctggccga gccctagccg gaggcgtcgt aggaggggag gcgcaccctg
      361 aagaccgtcc gccccggccg gctctcgaag ccgatggagc cgccgtggga ctcgacgatg
      421 cgcctgcaga tgtcgagccc gaggcccagg cccgagccct cctccttggt cgtgaagaag
      481 ggcgtaaaga tccggtcctt gaccgcgtcc ggtatgccgg accccgagtc ggcgaactcg
      541 acgacgacct cgccgccctc cctcagcacc gagatctcga gcctgccctt gccctccatc
      601 gcgtcccagg cgttgccgat gaggttgatc cagacctggt cgagctgctt gggccttccc
      661 ttgacccgca cgtcgcggtc gtagcggcgc aggacctcga cgccgcgctt gagcttgccg
      721 tgcatgaggg ccaggaccgt ctctatgccc gcgatcggat ccacgcgctg cgcctcgtcg
      781 gccgccgtcc cgtagccgaa ggagcgcagg gcgtccaccg tccaggcggc cttgtccgcg
      841 gagaggagga tgaggcgcga ggcgcgcagg gcgcccgaga gggcgtaggc cgactcgacg
      901 agttcggccg cgcgcgggga ctcgaggagg gccgagagcg cggcctcgtc gccctcgccg
      961 aagcccatgt ccgcgaggtc gtccgcgagg aggtcggcgt ccgccgcccc tcgctcctcg
     1021 aggagcccgc ggacgagggc gagggagcgc cgcctttcct cgtggcgggc ggggtcggcg
     1081 gccgagaggg ccctgcccac gaggccgcgg tagaggccca ggtcgcggtc ggagagctcc
     1141 gcggcgaagc cgggcaggcg ggcgagctcc tgctcgagga cctcggcgac gtggacggcg
     1201 ctcgagcgga tggccccgag gggcgtgttg agctcgtggg ccgtctccgc ggccaggcgg
     1261 cccagggccg cgagcttctc ggcgtccacg agcctgtcct gcgtcgcgcg gagctcgcga
     1321 ttcgcgccgg agagccgctc gttggccgcc tcgagcgacg ccgtcctctc cctcaccctg
     1381 tcctcgagct ccgagttgag ggcgaggatc ctttcgtccc tccgcctcgt ctcctccagg
     1441 gcccggccga tctcctcgcg ctttttcctg aaggagcggc tcaaggaagc tatctcctcg
     1501 aaggccagga gccgctcctc cacgggcggc ggtccgggct cgaggtccca gacctccagg
     1561 gcctcggcga agcgcgcgag cggggccagg gcgcgccggc tcagggcgaa gctccgcgcc
     1621 gcggccgcgg ccaggaaggc cagggccgcc gccggcccca ggctccagag gagggcgagg
     1681 ggggcggccc gctcgcgggc gggggagagc acgacgatcc tgtcgcccag ggcctcgtgc
     1741 agggcctcgc tcgcgagcca gccttcgccg aaggcctcga agggcaggtc gagcctggcc
     1801 gggaggaggt cgagcccgag gcccggccgc gtcgaggcga ggatcctgcc gtcggccgag
     1861 gcgaggagga tgacggtccc cgagtagtcg gcgatgcgcc tgagctcgcc caggatggcc
     1921 tcgacgcgga tgcggcccag gagccagccc gaggcgagcc tcgtcgcgag gtagacgctg
     1981 ggcctctcgt cctccacgcc ccgggccacg cccgagacgg cgacgccgaa gcctgagctc
     2041 cccgagccgg cgatgtagcg ctcgaccgtc ctcgaaagga gctctccggg ggcgccgtag
     2101 cccatgtcga agccctcgaa gacgccggag ccggggctct tcgcgaggat ggccgagacc
     2161 cggccgtcct ccccgacgag gtagaggtcg gagaagtcgt cgaccagggc gacggccgag
     2221 aggaggccgg gctcggcgcc taggcgcctg acaaggcccc tcccgcgctc gatgacggag
     2281 tcgacgagga cggtggcctt gagcgcctcc tcgctgcgcc tggccgcgta gaccgcgtcg
     2341 acgcggacgc tcgccgcgaa gagggcggcg aggagggcgg ccagggccgc cgcgaaggtc
     2401 gccgcctcca cggccagggt gtagccgcgg agggtcctcc ttctcaccgc agctccgcga
     2461 cgggattctc gatcatgtag aagggggagg aggagtcgcc ggccgcgtcg aagagggcct
     2521 cgcccaggct cgtcgggacc ctgcccttgc cgacgatcca gagcttgcag gcctcgggcg
     2581 tcgcggcccc cgagtcccag gccgcggcca ggacctccag ggccttgtag acgttgaggc
     2641 tgatgagggt cggggcgtag ccgaagcggg ccttgaagcg ggcgatgtag gcgtcgacct
     2701 cgggggagct gcccttgggc ggataatgcg agggcagggt ggagacggcg agggccggcc
     2761 ccgccgtcgc cgccagggcc ggcgagttga gccagggcgt gaagacgacg cgggcggccg
     2821 gcctcgccgc catggagagc tgggcgaagc ttcccgccgt cgcctggtag gccccgacga
     2881 ggacgtagag cgtgtcgtag ggcgtcgagg agacgacgga gcggatccgc tcgatgtcga
     2941 gggccgacat cctgatcctc tcctcggcga cgcgcttgcc gctccccagg gcggccttga
     3001 aggcctccag ggcggggagg gtgtaggcct cgttgtccag gtccaggatc acgaggacgt
     3061 ccctgcccgg gaggccggcg acgtagcggg ctatggcctc ctgctcgaag cggaggtcgg
     3121 gcacgtccct caagatgagg tcgtcctttc ccgaaaggag ggtcgtcgtg gagcccgtca
     3181 ccatcgcgag gacgccgtct tggcgcatcg gctcggccag cctgacggcg caggtggagg
     3241 tgtggctcgt cacgaagaag gagaagcctt cggcccgcgc cttcccgtag gccgccacgg
     3301 ccttgtcggg gtcccagccg tcgtcgaagg gcacgatctc gatgtcgcgg gccgggcggc
     3361 cggccgcgct cccgtcccgg gggccggcgc ccgctccgtt ccgcctggcc gccctgtcct
     3421 cgaggaagag ggtcgcggcg ttcacctcgc tcgccccgac gacggagccc gcctcgagct
     3481 tcgtgagcag ggccaccttg aggacgggcc gcccgcagga ggcgagcgcc agcgcggcga
     3541 ggaaaagcgc ggccggggcc gccgccctgg ccttggacca agacctttgt ctcttcatgc
     3601 cgccgccctc cgcccgccgc gcccccgcct agccggggaa ctggtcgagc ctgccttccc
     3661 tgaccacgcg gtgctcgccc gtggaggtgt ccaggtcgta gatcgagaaa ttcgtgtcga
     3721 tgtcgaagcc gggatgccgc acggcgaagt tgagcgaagc cgagccctcc gctcccgaga
     3781 cgatgcggtg gaagacgttg cagggccaga cgagcatcgc cgccccctcg aagaccacct
     3841 tgccgccgcg gagcacgcgc tcgggctcga ccacgaaggt ctccatccgc cggtactcga
     3901 ggctgtagat gtcgaccgtc ctcgtcccgt gcaggacgag gaggttgtcc tcctggtgcg
     3961 ggtggaggta ccagggccgc tcgaccccgc cgaccggtcc gggcgagagg gcttttgtct
     4021 ggtggatgac cctgtccacg ccgtcgatgc ggggcaggaa ctcgaggggg actatgtcga
     4081 aggagacgcc ctccgtcttc ctgaaggggc ggagcttgat gatccgccag aagcgggctt
     4141 cctcgtcgag caggtacttc acggcgctcc tccttggggg cggcgcgccc ccaccttcaa
     4201 gtgtagccgg gcggggccgg ccttcaagcc cgcttcggag aaggagtccc cgcctcagcc
     4261 cgcgtttccg ggcggcgact cgaggagctc cggatggagg acgaggcgct ggaagaggga
     4321 ggccgcgtgg gcgaagtaga tgccctcgga gatgcgctcg tcggcggtga tcttcacgtt
     4381 gatgaagtgg cgcttcgccg agcctcccgc gccgccgggc gcctccttct ggaacatccg
     4441 gcctaggacg acgaagaggc tcgccgtgcc ccactcgtag gcgtggtgga agggagcgtc
     4501 caggcccagg ctccccaggt tcgcgaagta ggcggaggcg aagaggggat ccccccggat
     4561 catcgaggcc ggcgcggcgt tgaagcggtc caggaggcgg aagagcgagg tgatgaccgc
     4621 cttgccccct ggaaggcggc ccacgaggtc gatctccctc tcgtcggcgc ccttgccctc
     4681 ctccttggcg gcggcgacgg cggcgttgaa cttgtccgtc gcctccgcga tcgtgtcctt
     4741 cgggtccagg aagagcttca cgttcgcctc cggcgcctcc tcggtgagcc gcttcttcac
     4801 gatgaaggag aaggcgatgt ggttgcgctc gtagatggcg cggcggtgca cgaagcggtt
     4861 gagctcgggc ttctccgcga tggtgcgcag ggccgcggcc aggacgaggc cgaagaggct
     4921 gtagcgggtt tggcccgact cggcgttctt ccggtgcacg taggcgatcg cgttctcgac
     4981 gtcgaggtcc ttcgagaagt agacggccga ctcgttccgg ccgcgcatga tgtagggcat
     5041 gatggcggag aggggcttga gcctccacag gcgcctgccg tcggggcgat cgacgaagat
     5101 catgcggaga ctataccccc gctcccgcgc gaggtaaagg cgagggccgg ccttcgggcc
     5161 ttggccgcga ggcgcggaat cggcgtaaag aatccccgtt cccttgcggc ccgggcccgg
     5221 cgcggtatac ttcacggaat gaaaatacga acgaggatgt tcctctcggt ggccgcgatc
     5281 cagggcctcc ttttcatagc cctcttctcc atcctcctcg tcctctcgac cggcacggcc
     5341 aggaaggccc tggtggaggc cgccgcgggc ggcgcgaagg cccgcgcgac gagggtcgcc
     5401 ctcaccctct cccggggcat agtctcctgc gggagcctcg tctccgacct gggcgccctg
     5461 cgctcctcgg gccgcctgga ccgcggcctc cccgtcctcc tcttcgagtc cgccttgcgc
     5521 cgcgagccct cggtcttcgc ctactgggcg atctacgaca agggcgcctg ggacggccgc
     5581 gacgccgccc tcgcggacga tcccgtcttc gcccccaccg gggcctacgt gccctgggcc
     5641 taccgcgagg agggcgggat caagtcgcag gccggcatgg agggcgacga ggacgtggag
     5701 ggctactacg gcgagttcta tacggtcccc ttcgcctcgg ggcggcccta cctctccgag
     5761 ccctacgccg aggaggtcga ggagggcaaa ttcgtcctca tgagcaccta cgccgagccg
     5821 ctcttcgacg ccgcggggcg gaagctcggc gtcgcgggct gcgacctctc cctcgattcc
     5881 ctgaccgccc tcgtccaggc ctccgcctcg gggaccgagg ccgcctccgg gaaggccggg
     5941 gccggtcttg cctactcggc cctcctgagc cccgagggct tcgtcctcgc ccacagctcc
     6001 gatcccgagc tcgtcggcaa gtccttcttc gaggccacgg ccgaggccgg cggagccgcg
     6061 ggcgaagcag gaagcgccgg ggccaaggac gccggaagcg ccgctggcgc cctggccgag
     6121 gacgcggcgg ccgtaatgaa ggcgatcgcc tcgggaagcg aggagctctg gcggcggaag
     6181 gtcccgggga gcaaggcgag cctactcttc gtggccgagc ccgtcgtcct gtccgcgggg
     6241 ctcccgccct ggatcctcct cgagtgcgtc cccgagaacc tcgtcctgga ggagggcgag
     6301 cggatgcgcg ccgtcatggc gatcttcctt gccctcggcc tcgtcacgat gggcttcggc
     6361 accttcgcca tcgcgacccg catctcccgc cccatagtcc gcctcacgga ggccttctcg
     6421 cggatggaag agggagacct caccttgcgc gcgcccgtcg cgagccgcga cgaggtgggg
     6481 aacctggcca agggcttcaa cctcttcgcc gtgcgcctct ccgccctcat gggctccttc
     6541 aggcgctcgg ccgaggagct ggacgcggcc gggcgccgcg tggcggaggc cgccgaccgc
     6601 acccgcgact ccctgggcgc catccgcggg ggcatggacc gggcccaggc ggaggtgcac
     6661 acgcaggtgg tctccgtggc ccagaccacg aaggcggcgg agggcatcgc ctcccgcatc
     6721 ggggacctcc gcgcggccgt cgaccggcag aacgacgccc tcgccgccgc cgcggccctg
     6781 gtcgagcgga ccatcgccca gctcggcgag atcgcccgga agtccgagtc catcaacgac
     6841 gagatgggcg ccctggaggg cgcgggaggt gaggggaagg ccaggctcga ggcggtgaag
     6901 acggccgtcc gcgaggcggc cgagcggagc gccgacctcc tggccgcgaa caaggtcgtc
     6961 gccgacgtcg ccgcgcggac gaacctcctc gcgatgaacg ccgccatcga ggccgcccac
     7021 gcgggcgagg ccggcaaggg cttcgccgtc gtcgcggcgg agatccgcgg cctcgcggag
     7081 aacacccacg gccagtccaa gtccatcgcc tcccgcgtcg cggagatcag ggccgcggtg
     7141 gcccgcgtga gcgactcggc gacggaggcg ggcgcctcct tcgacgagat catcggccgc
     7201 attcggcagg tggccggcct ggagggccag gcgagggagg ccctcttcga ggagagggac
     7261 ggcggcggca gggtcctgga agccatagcc gagacgcgcg aggccgccgg cctcgtggag
     7321 gcagcctccg cggagatggg ccgctctggc gaggaggtgc gcctggcgat ggcccgcctg
     7381 gccgaggcga gcgagggggt cgcagcgagc gcccgcgtgg tgtcgggccg cgtcgcggag
     7441 atcgaggcga gcgccgacga ggagcgctcc atcgcctctg agaacctccg catcgccgcg
     7501 ggcttccgcg aggaagtgag ccgcttcaag atctagcgcc gtccttcgcg ccggctccgg
     7561 gccttcccgc ggcgcggaag cctcggtatc atccagccat ggcctacgat tgcgtgatgc
     7621 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcggcgc ggaagcctcg
     7741 gtatcatcca gccatggcct acgattgcgt gatgccggcc gccggcgcct cgagccgcat
     7801 gggcgcctgg aagcccctcc ttccctggag gggggcgagc ctcgtcgagg cctgcgtcgg
     7861 cgccgccctc ggagcctgct cccgcgtgct cctggtcggc ggcgagcgcc tggccgagct
     7921 cgcctccctt tttcgcgggg agccccgcgt cctcgtcgtc gccaatccgg cctgggagct
     7981 cggcatgctc ggctccctgc agcgcgccct tcccctcgtg gagggcggcg ccttcttctg
     8041 cgcgaacgcc gacatgcccc tcatcgagtc cgcggactac gaggccctga aggcgcgata
     8101 cgcgatcctg gaggcctcgg gggctcccgc cgccgtcttc gccgcccacg gcggcgaggc
     8161 cggccatccc gtcctcgtcc cttcttccta catagccgag atcctcctcc ttcctcccgg
     8221 cggccgcatg aagagcttcc tctccgggaa gccctcctgc ctcgtcgagc gcggccccgg
     8281 ggtcctcgcc gacctcgaca cgccggacga ctacgagagg gccgcgcgcc ttgcgcccta
     8341 ggctcggctc ggggcggcgg aggcggggcc cgcccggcct cgctttcctg cccgcgcggc
     8401 ttggcgcggg ccagagcctc tccgccgccc gggcctcgcc cctcctgggc gcggccctcg
     8461 tcgtcctcgc ctcgggcttc ctctacgtct ccctcttcct cgccgtggcg cggatcggcg
     8521 ccgcggacga ggccctggag ctgcggctcg accgcgcgct ctactccctc ctgggagggg
     8581 cggcgggcga gagagcccca gtcggcgagg acctggacta cgtctaccag ctcctgcccc
     8641 gccttggcgg cgtcgactcg aaagtggcga tttcggaggc cgccgggcgc ctcgccgaat
     8701 ccatcgacgc ctaccgcgag gcccgggccg cggcggacgg ggccgctgct gcaggagccg
     8761 ctgctgacgg gcctgctgct gacgggaccg gaggagccgc cggcggagaa ggggaggccg
     8821 aagccgcgga gcgggcgcgc accgaactgg ccgcctcgat cgagagcttt ctccgcgtcc
     8881 tccacgcgcg cgggagcgag gtccgcgcct ccttcaacta cctcctcctc gcgagcggcg
     8941 tcggca
//
LOCUS       DAOOSJ010000097         8901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4979_length_8901_cov_7.367285, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000097 DAOOSJ010000000
VERSION     DAOOSJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_8901_cov_7.367285"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PLG14_06190"
     CDS             <1..1010
                     /locus_tag="PLG14_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOX48762.1"
                     /translation="NTDADVNVIALIGERGREVREFIENDLGEEGLRRSVVIVSTSDT
                     PPLARLRGAYVAAAVSEYFRDRGKDVMLLFDSVTRFARAQREIGLATGEPPATRGYTP
                     SVFDSMPKLLERSGTSDKGSITGIYTILVDGDDMDEPVADTVRGILDGHIVLSRKLAQ
                     RYHYPAVDVLKSVSRLTGAVSGPVTKTVMGYARKVMAVYEENEDMITIGAYVKGSSPQ
                     VDESIAKRDAVEDFLVQSVEDPAPVADTLRRLGGIAGVEIPPEEIAAAEGRLAVSPLA
                     PAAAVPAAAAPAAAPGGPAPDKAAARPAAEGGAEAGPAGGGVAALLRARVPPRSGEVE
                     P"
     gene            1007..1444
                     /gene="fliJ"
                     /locus_tag="PLG14_06195"
     CDS             1007..1444
                     /gene="fliJ"
                     /locus_tag="PLG14_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOX48763.1"
                     /translation="MRRFAFKLEKLLELRAFHEKRAELVLAEKAGRCAVLEAGLKEVA
                     ESRARTSREMFAPGRDLADYRAAELYLVRLDRERDRLLAELAQAELEREAARAEYIEK
                     HRDREAIDKLKERRQADYYRLAEREETKALDDIARRSLVEAGR"
     gene            1451..2083
                     /locus_tag="PLG14_06200"
     CDS             1451..2083
                     /locus_tag="PLG14_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HOX48764.1"
                     /translation="MASFGRPRVLGRAIVLLILILVLAIGGIVWFDFLGVVDAKGLLS
                     PAYRLLGIGARGQAPIPADSPVLLEEERMAKRLESLQARAEELDQREGEVARLDAEVG
                     QKAQELEERGKALDDKEKSFNEKVKQYDNRKVNVEQNAVYLSGMPPAKAVEILKAMED
                     QAVIDILRAVEERAKASGESSIVAYWLSLMPPDRSAAIQRKMTAKPSSLE"
     gene            3029..3415
                     /locus_tag="PLG14_06205"
     CDS             3029..3415
                     /locus_tag="PLG14_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOX48765.1"
                     /translation="MVLKDGDSGLIRLRMRPESLGNVKIELNLADNCISGKIVVESDE
                     AKSAFERNMAELGDAFRQGGFESAKLEVSVGSGGSGKGGAEGRAAEPEGPFWSERRRA
                     DAFGGGVPAAGGIRGSGALGAVDILA"
     gene            3428..3862
                     /gene="flgD"
                     /locus_tag="PLG14_06210"
     CDS             3428..3862
                     /gene="flgD"
                     /locus_tag="PLG14_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOX48766.1"
                     /translation="MELSTAMKGPDLAKTRVEVDSFNKALGGGKASTPNLDKDDFLKI
                     LITQLQHQDPTAPLEDKEFIAQMAQFSSLEQMTNMSQGFQKLSGLLASSEAAGVLGRN
                     VEVRDGESLVSGVVSQVVRGDFPLVGVNGKLYDFAAVEKVIY"
     gene            3876..5270
                     /gene="flgE"
                     /locus_tag="PLG14_06215"
     CDS             3876..5270
                     /gene="flgE"
                     /locus_tag="PLG14_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOX48767.1"
                     /translation="MMRSLYSGVAGLQNHQVRMDVLGNNIANVNTTGFKKGRVNFQDL
                     LYQQMSGASRPNAEVGGVNPKEIGLGMSIASVDTIHTQGALQTTGVMTDVAVQGNGFF
                     VLKKGEQTMFTRAGAFGLDRDGRLVNPGSGLRVQGWLAQNINGREVLSTSGQLEDLVI
                     PVGSKDPAKATSRVELACNLDKRTPEIPEGAGQDTVEEGTWRVEEKVYDSFGEAHILR
                     IEFRKAPGETNRWNATTTVDPGSETPTNAAVALGEPVAGNANAFTVEFDNRGLLRRVA
                     DAQGGLSPEEGALDIGVSFDVLGAAAGADGAPTRQTFRLALGRVGSATGTVTQQAERS
                     STKVFAQDGYTMGYLDSFKIDQQGIVTAVYSNGSNRTIGQMALASFTNPNGLEKIGET
                     NFVQSNNSGMANVGPSGIAGKGKLIAGTLEMSNVDLAEQFTDMIVTQRGFQANSKTIQ
                     TSDQMLQELLTLKR"
     gene            5357..5554
                     /locus_tag="PLG14_06220"
     CDS             5357..5554
                     /locus_tag="PLG14_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOX48768.1"
                     /translation="MIEVTQLSGKTFWLNPHQIEYIEKTPDTTVTMLGGKRVVVLESV
                     EAVIERIVAYRRLIGAFKNEE"
     gene            5557..6339
                     /locus_tag="PLG14_06225"
     CDS             5557..6339
                     /locus_tag="PLG14_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOX48769.1"
                     /translation="MNLGSLIGLVGGVVMILVSIFTSGGTVGTYMDLPSFSMTVGGSF
                     FALMLQYGMKETMQVFKLMGRAFKVYEFGAEKLLDKLYEFSEKARREGILSLEEEMND
                     LDDEFMQQGLRLVVDGTDAEVIRELMENELSQMNERHSVGIKQLDAWSKLAPGFGMLG
                     TVLGLIAMMKNLEDKSRIGPNMAVALITTFYGAIMANFLFQPMMGKLVIDDGAETTVK
                     EMIIEGVLSIQAGDNPRILLVRLASYLPPKQKKAILDKYKTE"
     gene            6349..7080
                     /gene="motB"
                     /locus_tag="PLG14_06230"
     CDS             6349..7080
                     /gene="motB"
                     /locus_tag="PLG14_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOX48770.1"
                     /translation="MAKKKKELSVGGIGEWVVTYGDMVTLLLCFFVALFNASDADVVQ
                     TEMLISSLNNIGIGGSTGGNTLSSGKLAELGNTVGTLPSMEKGKYLGTAMKKAVSLFQ
                     PEVKSNKVRVSSNERGIVISLASDAFFKPASAQINIEETRDLLVKLAGLLSADELKGR
                     RFAIEGHTDSSPVDPAGPWESNWELSAARAINTLHYLSDFGVEERRFRVAGFADTIPL
                     SSNDTEEGRAYNRRIDVIILDEAHL"
     gene            7258..7812
                     /locus_tag="PLG14_06235"
     CDS             7258..7812
                     /locus_tag="PLG14_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOX48771.1"
                     /translation="MGDNVDDQEMSFTEGEGQEAAAPKAKGVGPGILKILMIVGVALG
                     AVILIVTVVVITVGILNKQGKPMNQLSVSEEYQAATPIRAYFTTIGEIRARTRDKEPS
                     SVVIKLNVGFDMNDKETPNEITQRLYQVRDFLRSYFSMKYADELKPEDEDAIKEELRE
                     SLNRILSKPSIKEVLFEKFDVVGL"
     gene            7842..8876
                     /gene="fliM"
                     /locus_tag="PLG14_06240"
     CDS             7842..8876
                     /gene="fliM"
                     /locus_tag="PLG14_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOX48772.1"
                     /translation="MTEVLSQDEIDQLLTAINAGETEIETIKPAADTRKIKIYDFKRP
                     DKFSKEQIRTVQIMHETFARLTTTSLSASLRTLVHVHVASVDQLTYEEFIRSIPTPTT
                     LAVVSMDPLKGQAILEIDPSVTFTIIDRLFGGSGEGAKLNRELTDIEHSVMEGIIVRI
                     LGNMREAWTTVIDLRPRLSQIETNPQFAQIVPPTEMVVLVTLETKVGEVEGMMNLCIP
                     YLTIEPIVSKLSAQYWYSSVRRGATTENLNILKEKLSSVEIPVIAEIGKIDISVRDVL
                     ALRLGDIVRLYSVRVNDPMTLNVGNKKKFLCRPGVIGKKMAVQITKKLEDLGQEEFEE
                     LASEGDDNYE"
BASE COUNT         1635 a         3156 c         2908 g         1202 t
ORIGIN      
        1 ggaacaccga cgccgacgtc aacgtgatcg ccctgatcgg cgagcgcggc cgcgaagtgc
       61 gcgagttcat agagaacgac ctgggggagg agggcctcag gcgctccgtc gtcatcgtgt
      121 ccacctccga cacgcccccg ctcgcgcgcc tccgcggcgc ctacgtcgcg gcggcggtgt
      181 cggagtactt ccgcgaccgc ggcaaggacg tcatgctcct cttcgactcc gtgacgcgct
      241 tcgcccgcgc ccagcgcgag atcggcctcg ccaccgggga gccgcccgcg acccggggct
      301 acaccccttc cgtcttcgac tccatgccca agctcctcga gcgctccggc acctcggaca
      361 agggctccat caccggcatc tacacgatct tggtggacgg cgacgacatg gacgagcccg
      421 tcgcggacac cgtgcggggc atcctggacg gccacatcgt cctctcccgc aagctcgccc
      481 agcgctacca ctacccggcg gtcgacgtcc tcaagtccgt ctcccgcctc acgggcgcag
      541 tgtcggggcc cgtcacgaag accgtgatgg gctacgcgcg gaaggtcatg gccgtctacg
      601 aggagaacga ggacatgatc acgatcggcg cctacgtcaa gggctcgagc ccccaggtgg
      661 acgagtcgat cgccaagcgc gacgcggtgg aggacttcct ggtccagtcc gtggaggatc
      721 ccgcccccgt cgccgacacg ctgaggcggc tcggcggcat cgccggcgtc gagatccctc
      781 ccgaggagat agccgccgcc gagggccgcc tggccgtgtc ccccctcgcg ccggccgccg
      841 cggtcccggc cgccgcggcc ccggccgccg cccccggcgg accggcgcct gacaaggccg
      901 cggcgcggcc ggccgcggaa gggggcgccg aggccgggcc ggcaggcgga ggcgtcgcgg
      961 ccctgctccg cgcccgcgtg cccccgcgct cgggggaggt cgagccttga ggcgcttcgc
     1021 cttcaagctc gagaagctcc tcgagctcag ggccttccac gagaagcggg cggagctcgt
     1081 cctcgcggag aaggccggcc gctgcgccgt gctggaggcc ggcctcaagg aggtcgccga
     1141 gtcccgggcc aggacgagcc gcgagatgtt cgcgccgggg cgggacctcg ccgactaccg
     1201 ggccgccgag ctctacctcg tgcgcctgga cagggagcgc gaccgcctcc tcgccgagct
     1261 cgcgcaggcc gagctcgagc gcgaggccgc ccgggccgaa tatatagaga agcaccgcga
     1321 ccgcgaggct atcgacaagc tcaaggagcg gaggcaggcg gactactacc gcctcgccga
     1381 gcgggaagag accaaggccc tcgacgacat cgcaaggcga tccctcgtcg aggcggggag
     1441 ataatcgagc atggcctcct tcggcagacc gcgcgtcctc gggcgcgcca tagtcctcct
     1501 catcctcatc ctcgtcctcg ccatcggcgg gatcgtctgg ttcgacttcc tgggcgtcgt
     1561 cgacgcgaag ggcctcctct cgcccgccta ccgcctcctg ggcataggcg cgaggggcca
     1621 ggcccccatc cccgcggaca gtcccgtcct cctcgaggag gagcggatgg ccaagcgcct
     1681 cgagtccctg caggccaggg ccgaggagct cgaccagcgc gagggcgagg tggccaggct
     1741 cgacgccgag gtgggccaga aggcccagga gctcgaggag cgcggaaagg ccctcgacga
     1801 caaagagaaa tcgttcaacg agaaggtgaa acaatacgac aataggaagg tcaacgtcga
     1861 gcagaacgcc gtctaccttt cgggcatgcc gcccgcgaag gccgtggaga tcctgaaagc
     1921 catggaagac caggcggtca tcgacatcct gcgggcggtg gaggagcggg ccaaggcctc
     1981 gggcgagagc tcgatcgtcg cctactggct ctccctgatg ccgccggatc gctccgccgc
     2041 gatccagcgc aagatgacgg cgaagccgag ctccctcgag tagcctcgag gcgcgccaag
     2101 gcttcccgct ggcggaagcg aaaggagcgc gcctacgcaa cttcaggcac agactccggc
     2161 ccagggccag cggccggagg ccgcggggat ccagggcggc ccgggcggcg tcttcgccgc
     2221 caagggcagg aaggcggccg ccggccccgg cggcctcttc gccagcctcc tccaggcggc
     2281 caggaaggcc gagcccaagg aagcgcactc cggcgcggcc gcgaaggccg cggccggcga
     2341 gaccaaaaag acggaccaac agcgcaagcc cggttcgacc gcgataaagc catctcaggc
     2401 cgtcccgccc cacgaggcgg cgaaggccgg caagcccgcc gccaaggccg ccgaggggag
     2461 ccggcccgcc gctggcgcgc ccggcgccgc ctcgcgggcc gaggccgagg ccgaaaaggc
     2521 cctggccggc gcgaggcgcg gccaggccga caaggccagg cgcaggggcg agggcgagga
     2581 ggcagagcag gcctccgcgg ccgccctctc cctcgcggga gcccagcgaa gcacggcctc
     2641 caaggccaag gcggccgact cgcccgagga gggcctcgcc gccgcgcgcg cgaaggactc
     2701 cgccaaggcc tcccccgagc cgcgcgtcac cgtcctggac cagcgccgcc tggacgcgaa
     2761 gcgcgaggcg cggcgcaagg agggctccga agccgaggcc cgcgggtccg aggccgcgaa
     2821 gcccgaggcg ccccaggacg cccccaagcc ctccggccgc gagatcgccc tggacctggg
     2881 cggcgtcgga gggacggcct cccgcggcga gggcagcgga gcctcgggag aggccgcgcc
     2941 ggccgccgcg cccaagggcg acttctccgc cctcctcgcg gagcgcctca aggaagcctg
     3001 gaacggcgag atagtccaga gcgcgcacat cgtcctcaag gacggcgact cgggcctcat
     3061 ccgcctcagg atgcggccgg aatcgctcgg gaacgtcaag atagagctga atctcgccga
     3121 caactgtatc agcggcaaga tcgtcgtcga gtcggacgag gccaagagcg ccttcgagcg
     3181 caacatggcc gagctcggcg acgccttccg gcagggaggc ttcgagtcgg ccaagctcga
     3241 ggtctccgtc ggctcgggag gctcgggcaa gggcggcgcc gaagggcgcg cggccgagcc
     3301 cgaagggcct ttctggtccg agcggcgccg cgccgacgcc ttcggcgggg gagtccccgc
     3361 cgctggcggg atccgcggat cgggggccct cggggccgtc gacattctgg cctagggagg
     3421 aagcaaggtg gaactgagca ccgccatgaa gggccccgac ctcgcgaaga cgcgggtcga
     3481 ggtggattcg ttcaacaagg ctctgggcgg agggaaggcg tcgacgccca acctggacaa
     3541 ggacgacttc ctcaagatcc tcatcaccca gctccagcac caggatccga ccgcccccct
     3601 cgaggacaag gagttcatcg cccagatggc gcagttctcc tccctcgagc agatgacgaa
     3661 catgagccag ggcttccaga agctctcggg cctcctcgcc tcgagcgagg cggccggggt
     3721 cctcggacgg aacgtcgagg tccgcgacgg ggagtccctc gtctcgggcg tcgtctccca
     3781 ggtcgtgcgc ggggatttcc ccctcgtcgg cgtcaacggc aagctctacg atttcgcggc
     3841 cgtcgagaag gtaatctact aggagggcta gtcccatgat gcgttcgctc tattccggcg
     3901 tcgccggcct ccagaaccac caggtccgga tggacgtcct cggcaacaac atcgccaacg
     3961 tcaacacgac gggcttcaag aaggggaggg tcaacttcca ggacctcctc taccagcaga
     4021 tgtcgggagc ctcccggccc aacgccgagg tcggcggcgt gaacccgaag gagatcggcc
     4081 tcggcatgtc gatcgcctcc gtggacacca tccacaccca gggcgccctc cagaccacag
     4141 gcgtgatgac cgacgtcgcg gtccagggca acggcttctt cgtcctcaag aagggcgagc
     4201 agacgatgtt cacccgcgcc ggcgccttcg gcctggacag ggacggccgc ctcgtcaacc
     4261 ccggctcggg cctccgcgtc cagggctggc tggcgcagaa catcaacggc agggaggtcc
     4321 tctcgacctc cggccagctc gaagacctcg tgatccccgt cggctccaag gacccggcca
     4381 aggccacctc ccgcgtggag ctcgcctgca acctggacaa gcgcaccccg gagatccccg
     4441 agggcgccgg ccaggacacg gtggaggagg gcacctggcg cgtggaggag aaggtctacg
     4501 actccttcgg cgaggcccac atcctcagga tcgagttccg gaaggccccc ggcgagacga
     4561 accgctggaa cgccaccacg accgtggacc cgggctcgga gacgccgacc aacgcggccg
     4621 tggccctggg cgagcccgtc gcgggcaacg cgaacgcctt caccgtcgag ttcgacaacc
     4681 gcggcctcct ccgccgcgtc gccgacgcgc agggcggcct ctcccccgag gagggagccc
     4741 tggacatcgg cgtctccttc gacgtgctgg gcgcggccgc cggagcggac ggcgcgccta
     4801 ccaggcagac cttccgcctg gccctcggcc gggtcggctc ggcgacgggc accgtcaccc
     4861 agcaggcgga gcgctcctcc acgaaggtct tcgcccagga cggctacacg atgggctacc
     4921 tcgactcctt caagatcgac cagcagggca tcgtgaccgc cgtctactcc aacggctcga
     4981 accggaccat cggccagatg gcgctcgcga gcttcacgaa ccccaacggc ctcgagaaga
     5041 tcggcgagac gaacttcgtc cagtcgaaca actcgggcat ggccaacgtc ggcccctccg
     5101 gcatcgcggg caagggcaag ctcatcgcgg gcaccctcga gatgtcgaac gtcgacctgg
     5161 cggagcagtt caccgacatg atcgtcacgc agcgcggctt ccaggccaac tccaagacga
     5221 tccagacgag cgaccagatg ctccaggagc tcctgacgct gaagcggtag tagccacgcg
     5281 cctccgggcg cctgaaggcc gctagccgcg gccaagggcg cccgggggcg gcgcgggcgt
     5341 ccgtcccgga ggatccatga tcgaagtgac ccagctttcc ggcaagacct tctggctgaa
     5401 cccccaccag atcgagtata ttgagaagac tcccgacacc acggtcacga tgctgggcgg
     5461 gaagcgcgtc gtggtcctgg agagcgtcga ggcggtgatc gagcggatcg tcgcctaccg
     5521 gcgcctcatc ggggccttca agaacgagga gtgagcatga acctgggcag tctcatcggc
     5581 ctggtcggcg gcgtcgtgat gatcctcgtc tcgatcttca cctcgggcgg caccgtcggc
     5641 acctacatgg acctcccctc cttctccatg acggtgggcg gctccttctt cgccctcatg
     5701 ctccagtacg gcatgaagga gacgatgcag gtcttcaagc tcatgggccg ggccttcaag
     5761 gtctacgagt tcggcgcgga gaagctcctg gacaagctct acgagttctc ggagaaggcc
     5821 aggcgcgagg gcatcctctc cctggaggag gagatgaacg acctggacga cgagttcatg
     5881 cagcagggcc tcaggctcgt cgtcgacggc acggacgcgg aggtcatccg cgagctcatg
     5941 gagaacgagc tctcccagat gaacgagcgc cattcggtgg gcatcaagca gctcgacgcc
     6001 tggtccaagc tcgcgccggg cttcggcatg ctcggcacgg tcctcgggct catcgccatg
     6061 atgaagaacc tggaggacaa gagccgcatc ggcccgaaca tggccgtcgc cctcatcacg
     6121 accttctacg gcgccatcat ggccaacttc ctcttccagc cgatgatggg caagctcgtc
     6181 atcgacgacg gggcggagac caccgtcaag gagatgatca tcgagggcgt cctctccatc
     6241 caggcgggcg acaatccccg catcctcctc gtcaggctcg cgagctacct gcccccgaag
     6301 cagaagaagg cgatcctgga caagtacaag acggagtagg ggcgcgccat ggccaagaag
     6361 aagaaagagc tgtccgtcgg cggcatcggc gagtgggtcg tcacctacgg cgacatggtc
     6421 accctcctcc tctgcttctt cgtggccctc ttcaacgcga gcgacgccga cgtcgtccag
     6481 acggagatgc tgatctccag cctcaacaac atcggcatcg gcggctccac gggcggcaac
     6541 accctctcct ccggcaagct cgccgagctc gggaacacgg tcggcaccct gccctccatg
     6601 gagaagggga agtacctggg gacggcgatg aagaaggccg tctccctctt ccagcccgag
     6661 gtcaagtcga acaaggtccg cgtctcctcc aacgagcgcg gcatcgtgat cagcctcgcc
     6721 tccgacgcct tcttcaagcc ggcgagcgcc cagatcaaca tcgaggagac ccgcgacctc
     6781 ctcgtgaagc tggccggcct cctctccgcc gacgagctca agggccggcg cttcgcgatc
     6841 gaggggcaca cggactcctc gcccgtcgat cccgccggtc cctgggaatc caactgggag
     6901 ctctcggcgg ccagggccat caataccctc cattatctct cggacttcgg ggtagaggag
     6961 cgccgcttcc gcgtcgcggg cttcgccgac accatccccc tctcctccaa cgacaccgag
     7021 gagggcaggg cctacaaccg ccggatcgac gtcatcatcc tggacgaagc gcatctctag
     7081 gccctgaatg gccttgtttc gcgggcccgc ggcgcttttc agcgtcgcgg gccttgtgta
     7141 tactaatgtc ggagcggcgc cttccgataa tcggaaggag ggtttcaggg ctgacggccc
     7201 gggcgcgtcg cgcgcctggg cggccggccc cgccccgaga gcctcaggag gaagcggatg
     7261 ggcgacaacg tcgacgatca ggaaatgagc ttcacggagg gcgagggcca ggaggccgcg
     7321 gctccgaagg cgaagggggt cggccccggc atcctcaaga tcctcatgat cgtgggcgtc
     7381 gccctgggag ccgtcatcct gatcgtgacg gtggtcgtca tcaccgtcgg catcctcaac
     7441 aagcagggaa agccgatgaa ccagctctcc gtctccgagg agtaccaggc ggccaccccg
     7501 atcagggcct acttcaccac gatcggcgag atccgcgccc gcacccggga caaggagccc
     7561 tcctccgtcg tcatcaagct caacgtgggc ttcgacatga acgacaagga gaccccgaac
     7621 gagatcacgc agcgcctcta ccaggtgcgc gacttcctcc gctcctactt cagcatgaag
     7681 tacgcggacg agctcaagcc ggaggacgag gacgccatca aggaagagct ccgcgagagc
     7741 ctaaaccgca tcctctcgaa gccgagcatc aaggaagtgc ttttcgagaa attcgacgtc
     7801 gtcgggctct aggcccggcg cgagaggtcc gtggaggacg catgaccgaa gtcctgtccc
     7861 aggatgagat agaccagctc ctcaccgcca tcaacgcggg cgagaccgag atcgagacga
     7921 tcaagccggc cgccgacacc cgcaagatca agatctacga cttcaagcgg ccggacaagt
     7981 tctccaagga gcagatccgc accgtccaga tcatgcacga gaccttcgcc cgcctcacga
     8041 cgacgagcct ctcggccagc ttgcgcaccc tcgtccacgt ccacgtcgcc tccgtcgacc
     8101 agctcaccta cgaggaattc atccgctcca tcccgacgcc caccaccctg gccgtcgtca
     8161 gcatggaccc cctcaagggc caggccatcc tggagatcga cccctccgtc accttcacga
     8221 tcatcgaccg cctcttcggc ggctcgggag agggcgcgaa gctcaaccgc gagctcaccg
     8281 acatcgagca ctccgtcatg gagggcatca tcgtccgcat cctcggcaac atgcgcgagg
     8341 cctggaccac ggtcatcgac ctcaggcccc gcctctcgca gatcgagacg aacccccagt
     8401 tcgcccagat cgtcccgccc acggagatgg tcgtcctcgt gaccctggag accaaggtgg
     8461 gcgaggtcga gggcatgatg aacctctgca tcccctacct cacgatcgag cccatcgtct
     8521 ccaagctctc cgcccagtac tggtactcct cggtgcgccg cggcgcgacg acggagaacc
     8581 tcaacatcct caaggagaag ctatcctccg tggagatccc cgtgatcgcg gagatcggca
     8641 agatagacat ctcggtccgc gacgtcctgg ccctccggct cggggacatc gtgcggctct
     8701 actcggtccg ggtgaacgac ccgatgaccc tcaacgtggg aaacaagaag aaattccttt
     8761 gccgccccgg cgtgataggc aagaagatgg cggtgcagat aacgaagaag ctcgaggacc
     8821 tcgggcagga agagttcgaa gaactggcat ccgaaggaga cgataactat gagtgacggc
     8881 gccctctccc aggaagagat a
//
LOCUS       DAOOSJ010000098         8887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_4989_length_8887_cov_6.590014, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000098 DAOOSJ010000000
VERSION     DAOOSJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_8887_cov_6.590014"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLG14_06245"
     CDS             <1..776
                     /locus_tag="PLG14_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48773.1"
                     /translation="AAIAANDGTSLRIEALARPFGVEVFRAETGEANVVGLAAELRSR
                     GYAVRILGEGSNGGNITEPSEVRDPLATLGALLKLLLLRDSEKGEGLFRIWMRLSGRL
                     ADYRPDFGLADVVASLPSFATTSAFEPRAALRIRSSDQAALKARYRDLFLAEWPARRP
                     ELERRLGVVSWKALASSGPAEREIGDDFAASGKGGLKILLLDASGSPKAFLWMRGSGT
                     EPVFRVMADVEGGRGRDPAEDEAYLLDWHTSMVRRADEA"
     gene            950..1366
                     /locus_tag="PLG14_06250"
     CDS             950..1366
                     /locus_tag="PLG14_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOX48774.1"
                     /translation="MGIRGELFSTRISFEGRTYFFNVKENRMGDVFLSIVESKPTEAE
                     NFDRRSIVIFRDDMRGFLKAFQGALKYMEKPGKEASSLSYEPEEDSRPAPRRRSPEAA
                     PSGEKKRRVVVRRKEAAKDEAKDAEPSGDSPADDEE"
     gene            complement(1448..1933)
                     /locus_tag="PLG14_06255"
     CDS             complement(1448..1933)
                     /locus_tag="PLG14_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48775.1"
                     /translation="MSAFWPAPSRSAFGQSSQAGEPRRISASELERLAEISRRLSLIN
                     ERLKTELEDSRRSSEDLAALLESSRRELEALRLELEASRTISTGLASRAESSERDSAS
                     LREALTRAQVSLESSTTSFEAYRRAAEARLRRAERGKRLLALAAASGWAAALGLLLFG
                     G"
     gene            complement(2336..2941)
                     /locus_tag="PLG14_06260"
     CDS             complement(2336..2941)
                     /locus_tag="PLG14_06260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOX48776.1"
                     /translation="MRASDFPEGPPLGPGVCGIDEAGRGPLAGPVYAAAAVLPADFPL
                     EVLGDSKALSERRREEAFALIVERALAWAVDWASPAEIDERNILGATFLAMGRAYGAL
                     EASLRGRGLAIPPGLYVDGNRLPPLGRQAVAVVKGDAIVPAIMAASILAKVARDRAME
                     RLDWLYPEYGYARHKGYPTAAHREALRRLGPSPAQRSSFQY"
     gene            3132..3992
                     /locus_tag="PLG14_06265"
     CDS             3132..3992
                     /locus_tag="PLG14_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48777.1"
                     /translation="MDLSFYKLRVCEADLLLVDDLAGKGRDRDWAAAARSLLDRRRGA
                     GADRLAVLSKAEEAVWLRAFDREGEPAACLFDAALCAARYLLDSGRSGSESVELRVPR
                     GSLVVDVLDASSLGLSLGPPRGLPSGEALDLGAAAARKSLIEAGGERYEVLPLCIGGR
                     GAQEDETRPGERAETVAVFCEGGTRKVKARIGASARGAKAPPPLAVRLVARGELWAEI
                     PRGLDAAATAGLALAAAAAAGYADREAVVRLPGGAVFVEWAESGSLYAAARPEYAYRG
                     EFHLDEPEGE"
     gene            complement(4104..5183)
                     /locus_tag="PLG14_06270"
     CDS             complement(4104..5183)
                     /locus_tag="PLG14_06270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48778.1"
                     /translation="MSEAAWRRAGREDLPALEAYLRSREPEAAGFIGRLLRDGRLRLP
                     NPLRGAVYVLEEAGGEALGGALLASPTRAVFPLLDGARGSAEGRTGGSARRAAGGRHA
                     GGASAASADSDRGLADLLLCRGYEPVSIVGLAAEVERLEAALGLMPLVPVSYRLMARP
                     ASAGAASSASPRPAAETRARDSRHSAAEAGPAAAPPSPASPRPSPAARADAGPPTGAC
                     RTRLAGPADLDELYPLQEAYEKEEVLTLIHRFEPAATRAALARSLEERIVVAAEVGGF
                     LVGKAGTNARAFGLDQVGGVYVAPPYRGRGIGRALMEDLLGRLASEGRGASLFVKTHN
                     EPARGLYSSLGFEDLADFRADYFAS"
     gene            complement(5180..6724)
                     /locus_tag="PLG14_06275"
     CDS             complement(5180..6724)
                     /locus_tag="PLG14_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX48779.1"
                     /translation="MSDEEVLGQLFMLAYPGDTPPPLLFDWIGKRGLGGIKIFGWNAE
                     DTTKVAEAVAALQKAALASGRKVPLLIATDQEGGWIRHVKGATSQTPGNMAIGASGRP
                     YDAYWSGYYIARELEALGINMNFAPAVDLATRPRSSIIGPRAFSDDPEESAALGVAYY
                     RGTAAAGLIATAKHFPGHGDTELDSHGTLPVIRTGEKTLWERELVPFRALAAEGVPAV
                     MSGHLNFPLVSGDSLPASLSRHFMTDILRGRLGFQGVAVTDDLMMAGAAGAGASSLSE
                     AFYLAVEAGNDLLESSRIMALDDPAWTRLLSAYRSKPAFRARVREAARRVLVLKLDYL
                     RPKGPKALVPDPGRLGERLPDREGEAFFRGQAFRGASALDAERLPWKPSGKILVAAPF
                     ADFLEAAAAAFPGAARHKFSYRPESAALPEELAAFERAAASADAVLVCAPNEAGMDFY
                     ERARALGKRVAVVSVFSPAPLSRCGPEDAAVAVYGNSRACFDAGLAALKGEEAAEGRL
                     PVKLSR"
     gene            complement(6967..7902)
                     /gene="trxB"
                     /locus_tag="PLG14_06280"
     CDS             complement(6967..7902)
                     /gene="trxB"
                     /locus_tag="PLG14_06280"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOX48780.1"
                     /translation="MESEFDLIIVGGGAAGLAAAQYGARANLKTLLVEEMAPGGQALL
                     IDGLENYPGIPEPVSGYEFAENMRRQAERFGAGFLTASVSALRKEGKRFLLETSEGNL
                     QALAVILATGAKHRHLEVPGEERLSGRGVSYCATCDGPFFKGKRILVVGGGDAACDEA
                     MFLAKLSDQVTMVHRKDRFRAQRALAERVLKNPRVSVRFETVVAEIKGEKKVESVVLE
                     KVGSGERSEEPFDAVFVFVGSLPQTRLAPEAKKDEAGSLLTDELMATSVPGLFAAGDV
                     RATPFRQLVTACADGALAANSAAKYIDELRGEAYV"
     gene            7920..>8887
                     /locus_tag="PLG14_06285"
     CDS             7920..>8887
                     /locus_tag="PLG14_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48781.1"
                     /translation="MTSIARGRYIEHTETSTRTTRSPVLDLTDSRARPRSRPERRGPP
                     LLALLVLALLGQAAGAGASPALPPADAAARKAAEEYRLLLLPEPELAAIGALAEPLPL
                     ALLARASLLASGLGPGPEAAPGAEAAAAARLASYEARLGRLLEELAAAVEAPPPQGSA
                     RPRSEAEKAEAALAYLHAKVFRRYELDATTIDLVLDEGRYNCVSSALLYLLAMKRLGI
                     EAAGVRTEDHAFCLVRTDGREIDVETTNAYGFDPGRKKEFADAFGKVTGYSYVPPGAY
                     ARRWTMGERELASLVLSNRAAVLDAKGRFREALSLGASYAALRGGLG"
BASE COUNT         1178 a         3286 c         3334 g         1089 t
ORIGIN      
        1 aggcggcgat cgcggcgaac gacggcacga gcctcaggat cgaggccctg gccaggccct
       61 tcggcgtcga ggtcttccgc gccgagaccg gggaggccaa cgtggtcggc ctcgccgcgg
      121 agctcaggtc gaggggctac gccgtgcgca tcctgggaga gggctcgaac ggcggcaaca
      181 tcaccgagcc ctcggaggtc cgcgaccccc tcgccaccct gggcgccctc ctcaagctcc
      241 tcctcctcag ggactcggag aagggcgagg gcctcttcag gatctggatg cggctctccg
      301 gccgcctcgc cgactaccgg cccgacttcg gcctcgccga cgtcgtcgcc tccctgccct
      361 ccttcgcgac gacgagcgcc ttcgagccga gggccgccct gcgcatacgg agctccgacc
      421 aggccgccct caaggcccgc taccgcgacc tcttcctggc cgagtggccc gcgcgccgtc
      481 cggagctcga gcgccgcctg ggcgtcgtct cctggaaggc cctggcctcg agcggcccgg
      541 cggagcgcga gatcggcgac gacttcgcgg cctcgggcaa gggcggcctc aagatcctcc
      601 tcctcgacgc ctcgggctcg cccaaggcct tcctctggat gcgcggctcg ggcaccgagc
      661 ccgtcttccg cgtgatggcc gacgtcgagg gcggccgggg ccgcgatccg gccgaggacg
      721 aggcctacct cctcgattgg cacacgtcga tggtgcgcag ggccgacgag gcctgacggc
      781 ctccgcccag gcggggcggc gcggcgcgct ccgccggaaa ggcgccccgc caacaaagcg
      841 caccgccggc aaggcggcgc ggcgcgcccc gagggacgcc gccgcctctc cttgccgagg
      901 gccgcggaat ccggtaagat acgcgaagga aggcggcaga ggagaaccta tgggcatacg
      961 cggcgagctc ttttcgacga ggatcagctt cgaggggcgg acctacttct tcaacgtgaa
     1021 ggagaaccgc atgggcgacg tcttcctctc catcgtggag tcgaagccca cggaggccga
     1081 gaacttcgat cgccgctcca tcgtcatctt ccgcgacgac atgcgcggct tcctcaaggc
     1141 cttccagggc gccctcaagt acatggagaa gcccggcaag gaagcctcct ccctgagcta
     1201 cgagccagag gaagactccc gccccgcccc ccgccgccgc tctcccgagg ccgcgccctc
     1261 cggcgagaag aagcgccgcg tcgtcgtgcg ccgcaaggaa gcggccaagg acgaggcgaa
     1321 ggacgccgag ccctcgggcg attcccccgc cgacgacgag gagtaggccc cgccttccgg
     1381 caaggcgccc aggctccgac tacggtccgg cccccgcggg ccggccgccc gctccgtccc
     1441 ccgcaagcta gcccccgaac agcagcaatc ccagggccgc cgcccaaccc gaggccgcgg
     1501 cgagggccag gaggcgcttc ccccgctcgg ccctcctaag gcgggcctcc gccgccctcc
     1561 tgtaggcctc gaacgaggtc gtcgagctct cgagcgagac ttgcgccctc gtcaaggctt
     1621 cccgcagcga ggccgagtcc cgctccgagc tctccgcacg cgaggcgagc ccggtcgaga
     1681 tcgtccgcga ggcttccagc tcgagcctca gcgcctcgag ctcgcgcctc gagctctcga
     1741 gcagcgcggc caagtcttcc gagctccgcc tcgagtcctc gagctctgtc ttgagcctct
     1801 cgttgatcag cgagagccgc ctcgagatct cggcgagccg ctcgagctcg ctcgccgaga
     1861 tccgccgcgg ctcgccggcc tgcgagctct gtccaaaggc cgaccgcgaa ggcgccggcc
     1921 agaaggccga caagagcgcc gcgaaggaaa gcgcgacgac gcgcggagcc ggaccgagcg
     1981 gctcccgccg gcgcggctcc cgccggcgcg gcgcccgcgt ctggcggctc ggctccgccc
     2041 ggccgcgcga cgtccgcggc cgcgtcagac ggcgcggcgc ccgctcccgt cccgccttcc
     2101 cgtccggcgc ctgtccgggc gcgcccgcct ccgccgccgc ttcccggcgt ccatcgcttt
     2161 cgcacatcga gcctcccctg tcggccgtcc gcgcgccttc gcgcgctcac gcgagacgcg
     2221 gcgcggcgcc gcgcctgcag aaagcataga ggccgccgga cggggagtca aagcccgggc
     2281 cgcgcggccc cgcgccgccg cctgcggcag ctggccgacc cgccgtggaa gcccttcaat
     2341 attggaagct cgagcgctgg gcgggcgagg ggccgaggcg gcgcagggcc tcgcggtgcg
     2401 ccgccgtggg atagcccttg tgccgcgcgt agccgtactc ggggtagagc cagtccaggc
     2461 gctccatggc cctgtccctg gccaccttgg cgaggatgga ggccgccatg atcgcgggca
     2521 cgatggcgtc tcccttcacg acggcgacgg cctgcctccc gaggggcggc aggcggttcc
     2581 cgtccacata gaggccggga gggatcgcga ggccgcgccc gcgcaaggaa gcctcgaggg
     2641 ccccgtaggc gcggcccatc gccaggaagg tggcccccag gatgttcctc tcgtcgatct
     2701 cggccgggga ggcccagtcg acggcccagg cgagggctcg ctcgacgatg agggcgaagg
     2761 cctcctcgcg gcggcgctcg gacagggcct tcgagtcgcc caggacctcc agggggaagt
     2821 ccgcgggcag cacggcggcg gcggcgtaga cgggtccggc cagggggccc cgtcccgcct
     2881 cgtcgatgcc gcagacgccg ggcccgaggg gcggaccctc cgggaagtcc gaggcgcgca
     2941 agacggtcgc gcgcggcctg gctccggaga gcgccttggg accggccttg gtcgcgcgct
     3001 cgggcgagcg gtcgggcagg tcgtcgaaaa gaaagtcgcg catcggggaa agcatagccc
     3061 gccgcgcggg ccgcagtcca cgggccgcgc cgccgcttcc gccgcggctc cggcctcgat
     3121 acaatgcccg catggacttg agcttctaca agctccgcgt ctgcgaagcc gacctcctcc
     3181 tcgtcgacga cctcgcgggc aagggcaggg accgggactg ggcggccgcg gcgagatcac
     3241 tcctggaccg gcggcgcggc gcgggggcgg accgcctcgc cgtgctctcg aaggccgagg
     3301 aggccgtctg gctccgcgcc ttcgaccggg agggcgagcc cgcggcctgc ctcttcgacg
     3361 ccgccctctg cgccgcgcgc tacctcctcg actcaggcag gagcggcagc gagtcggtgg
     3421 agctccgcgt gccccgcggc tccctcgtcg tcgacgtcct ggacgcctcg agcctgggcc
     3481 tgtccctggg gccgccgcgg ggcctgccct cgggcgaggc cctcgacctt ggggcggccg
     3541 ccgcgcgcaa gtccctcatc gaggccggcg gcgagcgcta cgaggtcctc cccctctgca
     3601 tcggcggccg cggcgcccag gaagacgaga cgcggcccgg cgagagggcg gagaccgtcg
     3661 ccgtcttctg cgagggcggg acgaggaagg tcaaggcgag gatcggcgcc tcggcccgcg
     3721 gcgcgaaggc cccgccaccc ctcgcggtgc gccttgtcgc gcgcggcgag ctctgggctg
     3781 agatcccccg cggcctggac gcggcggcga cggcgggcct cgccctggcc gcggcggccg
     3841 ccgccggcta cgccgaccgc gaggccgtcg tgcggctccc tggcggcgcc gtcttcgtcg
     3901 agtgggccga gtcgggctcg ctctacgcgg ccgcccgccc cgagtacgcc taccggggcg
     3961 aattccacct ggacgagccc gagggcgaat agggcccgca cggcgggctt cacggcgcgg
     4021 cgggcttcac ggcgcggcgg ccttcacggc acggcgaacc atcgcggcgg gcaggaaggg
     4081 ccggcccggg ccgcgagacc ggcctaggag gcgaagtagt ccgcgcggaa gtcggccagg
     4141 tcctcgaagc ccagggagga gtagaggccg cgcgccggct cgttgtgcgt cttcacgaag
     4201 agggaggctc ccctcccctc gctcgccagg cggcccagga ggtcctccat gagggcccgg
     4261 cctatcccgc ggccgcggta gggaggcgcc acgtagacgc cgcctacctg gtccaggccg
     4321 aaggcgcggg cgttcgtgcc cgccttgccc acgagaaagc cgcccacctc ggcggcgacg
     4381 acgatcctct cctcgaggct ccgcgccagg gcggcgcggg tcgcggccgg ctcgaagcgg
     4441 tggatcaggg tgaggacctc ctccttctcg taggcttcct ggaggggata gagctcgtcg
     4501 aggtcggccg ggccggcgag ccgcgtcctg caggctccgg tcggaggccc cgcgtcggcg
     4561 cgcgccgcgg gcgaggggcg gggcgaggca ggcgaaggcg gcgcggccgc ggggcccgct
     4621 tcggccgcgg aatgccgcga gtcgcgcgcg cgggtctcgg ccgcggggcg gggcgaagcc
     4681 gaggacgcgg cgccagccga agccggcctt gccatgaggc ggtaggagac gggcacgagg
     4741 ggcatgaggc cgagggccgc ctcgagccgc tcgacctcgg ccgcgaggcc gacgatggag
     4801 acgggctcgt agccccggca gaggaggagg tcggcgaggc cgcggtcgga gtcggcgctg
     4861 gccgccgacg cgccgccggc gtggcggccg ccggccgcgc ggcgggcgga gcccccggtc
     4921 ctgccctcag ccgatccgcg cgcgccgtcc aggaggggga agaccgcgcg agtgggcgag
     4981 gcgaggaggg ccccgccgag cgcctcgccc ccggcctcct ccaggacgta gaccgcgccg
     5041 cggagcggat tcgggaggcg gagcctcccg tcgcggagca ggcggccgat gaagcccgcg
     5101 gcctcgggct cgcgggagcg gaggtaggcc tcgagggcgg ggaggtcctc cctgccggcg
     5161 cgccgccagg ccgcctcgct catcgggaca gctttaccgg gagccggccc tcggcggcct
     5221 cctcgccctt gagtgcggcg aggcccgcgt cgaagcaggc gcgggagttg ccgtagacgg
     5281 cgacggccgc gtcctcgggc ccgcagcgcg agaggggggc cggggagaag acggagacga
     5341 cggcgacccg cttcccgagg gccctcgccc gctcgtagaa gtccatgccc gcctcgttcg
     5401 gggcgcagac aaggacggcg tcggccgagg cggccgcccg ctcgaaggcg gcgagctcct
     5461 cgggcagggc ggccgactcg ggcctgtagg agaacttgtg ccgcgccgcg ccggggaagg
     5521 ccgcggccgc cgcctccagg aagtccgcga agggggcggc gacgaggatc tttccgctcg
     5581 gcttccaggg caggcgctcc gcgtccaggg ccgaggcgcc ccggaaggcc tggccgcgga
     5641 aaaaggcctc gccctcccgg tcggggaggc gctcgccgag ccttccggga tcggggacga
     5701 gggccttcgg gcccttgggc ctgaggtagt cgagcttgag gacgaggacg cgcctggccg
     5761 cctcgcggac gcgggcgcgg aaggcgggct tggagcggta ggccgaaagg aggcgggtcc
     5821 aggccgggtc gtccagggcc atgatcctgc tcgactcgag gaggtcgttc cccgcctcca
     5881 cggcgaggta gaaggcctcg gagaggctcg aggccccggc gccggccgcg cccgccatca
     5941 tgaggtcgtc ggtgaccgcg acgccctgga agccgaggcg cccgcggagg atgtccgtca
     6001 tgaaatgcct ggagagggag gcggggaggc tgtcgcccga gaccaagggg aagttgaggt
     6061 ggccgctcat cacggcgggc accccctccg cggcgagggc gcggaagggc acgagctcgc
     6121 gctcccagag ggtcttctcc cccgtcctga tgacgggcag ggtcccgtgg gagtcgagct
     6181 ccgtgtcgcc gtggccgggg aagtgcttgg cggtcgcgat gaggccggcc gccgccgtgc
     6241 cgcggtagta ggccacgccc agggcggcgc tctcctcggg atcgtcggag aaggcccggg
     6301 ggccgatgat gctgctcctg ggccgcgtcg ccaggtccac cgccggcgcg aagttcatgt
     6361 tgatgcccag ggcctcgagc tcgcgggcta tgtagtagcc cgaccagtag gcgtcgtagg
     6421 gccggcccga ggcgccgatg gccatgttgc caggggtctg gctcgtcgcg cccttcacat
     6481 gcctgatcca gccgccctcc tggtcggtcg cgatgaggag ggggaccttg cggccggagg
     6541 cgagggcggc cttctggaga gccgcgacgg cctccgccac cttggtcgtg tcctccgcgt
     6601 tccagccgaa gatcttgatg ccgcccaggc cccgcttccc gatccagtcg aagaggaggg
     6661 gcggcggcgt gtcgcccggg taggcgagca tgaagagctg gcctaaaacc tcctcgtcgc
     6721 tcatgccctc gagaagctcg tcggccctgg cgcccgggtc gccgggggcc cagaagtcgc
     6781 gggaaggccc cgcggccgag gagacgagga ggaaggcgca gagcgccagg ccgaggacgg
     6841 ccgccgcccc gggaaggcgc cggccgaggg gcccggacgc ggaccgggca cgggccggcg
     6901 cgcggcgttc gcccgggccg cggcgcccgc cgggatggcg gcgcgccggg gtttcgtccc
     6961 tagggctcag acgtaggcct cgccgcggag ctcgtcgatg tacttcgccg cggagttggc
     7021 ggccagtgcg ccgtcggcgc aggcggtgac gagctggcgg aagggggtcg cgcggacgtc
     7081 gcccgcggcg aagaggccgg ggacgctcgt cgccatgagc tcgtcggtga ggagggagcc
     7141 cgcctcgtcc ttcttcgcct cgggggcgag ccgggtctgg gggagggagc cgacgaagac
     7201 gaagacggcg tcgaagggct cctcggagcg ttcgcccgag ccgaccttct cgaggacgac
     7261 cgactcgacc ttcttctcgc ccttgatctc cgcgacgacg gtctcgaagc ggacggaaac
     7321 gcgggggttc ttgaggaccc gctccgcgag ggcgcgctgg gcccggaagc ggtccttccg
     7381 gtggaccatg gtcacttggt cggagagctt cgcgaggaac atcgcctcgt cgcaggccgc
     7441 gtcgccgccg ccgacgacca ggatgcgctt gcccttgaag aaggggccgt cgcaggtcgc
     7501 gcagtaggag acgcccctgc cggagagcct ctcctcgccc gggacctcga ggtgtcggtg
     7561 cttggccccc gtcgcgagga tgacggccag ggcctggagg ttcccctcgc tcgtctcgag
     7621 gaggaagcgc ttcccctcct tgcgcagggc ggagacggag gcggtgagga agccggcgcc
     7681 gaagcgctcg gcctggcggc gcatgttctc ggcgaactcg tagccggaga cgggctccgg
     7741 gatgccgggg tagttctcca ggccgtcgat gaggagggcc tggccgcccg gggccatctc
     7801 ctccacgagg agggtcttca agttggcgcg ggcgccgtac tgggcggccg cgaggccggc
     7861 ggccccgccg cccacgatga tgaggtcgaa ctcgctttcc actgtggtct cctgaatcga
     7921 tgacgagtat agcccgaggg cggtatattg aacatacgga aacatcgacg cgcacgacaa
     7981 ggagccctgt cctggacctg accgattcgc gggctcgccc ccgctcccga ccggagcgcc
     8041 gcggccctcc cctcctcgcc ctcctagtcc tcgccctcct cggccaggcc gctggcgcgg
     8101 gagcctcccc ggccctccct cccgccgacg ccgccgcgag gaaggccgcc gaggagtaca
     8161 ggctcctcct cctccccgag ccggagctgg ccgccatcgg cgccctggcc gagcccctgc
     8221 ccctcgccct cctcgccagg gcctccctcc tcgcctcggg cctgggcccc ggccctgagg
     8281 cggccccggg agcggaggcc gcggccgccg cccgcctcgc ctcctacgag gccaggctcg
     8341 gccgcctcct cgaggagctc gccgccgcgg ttgaggcacc gccgccgcag ggaagcgcca
     8401 ggccgcgcag cgaggcggag aaggccgagg cggccctcgc ctacctccac gcgaaggtct
     8461 tcaggcgcta cgagctggac gcgaccacga tcgacctcgt cctggacgag ggccggtaca
     8521 actgcgtctc ctcggccctc ctctatctcc tcgcgatgaa gcgcctgggc atcgaggccg
     8581 ccggcgtgcg caccgaggac cacgccttct gcctggtgcg gacggacggc cgcgagatcg
     8641 acgtggagac gaccaacgcc tacggcttcg accccggcag gaaaaaggag ttcgccgacg
     8701 ccttcggcaa ggtgacgggc tacagctacg tgccgcccgg ggcctacgcg aggcgctgga
     8761 ccatgggcga gcgggagctc gcctccctgg tcctctccaa ccgcgccgcc gtcctggacg
     8821 ccaaggggcg cttccgcgag gccctctccc tcggggcctc ctacgccgcc ctgcgcggcg
     8881 gcctcgg
//
LOCUS       DAOOSJ010000099         8753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5082_length_8753_cov_5.814670, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000099 DAOOSJ010000000
VERSION     DAOOSJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_8753_cov_5.814670"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2417)
                     /locus_tag="PLG14_06290"
     CDS             complement(<1..2417)
                     /locus_tag="PLG14_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOX48782.1"
                     /translation="MPFADTVGTVALVLLATLLVPIAAEKLRVPSLAALALAGALIGP
                     FGLGLPGEGRAVGLLGEIGLLYAFLLAGAGLDFVPLRKGRAPSALAAVLSFAIPFGSG
                     AAAASLLFGQSLLSSVLVGAAFAARSAKPYPAAAKLGLARSRSALAAAGGASLSETLA
                     VAALAAAGAAHAGSPFGADWPLRLGLGLGAAALLWLLLPLLASAFFKRVRVDGSVEFV
                     FVLATAFLCAFAAERSGLPSAVGAFFAGLLLRRFVPESSDLSAKLGFAGGAFFLPFFL
                     VYAGMNADLPGLAAAPGALLAALAAFGLLLAARALSSLLELPILRFTGEEAAFGFGLS
                     LNQGAAALGILLAGLRLGLVDGQLFGGALLVVVAAGVVGPVAAKAAGARLALRSGSIP
                     AGSGQEPERVMVALSNPATLQPLVDLAFLLRGPDEEEAVHPLAVAADSGDAEAELVKA
                     ENLLAKAMVLATRAGVPVKPATRLCVNVPEGILQAADEIRARILVVGWNRAPRLSSAF
                     FGSVIDRVVQGGRELVVVARAERPLKGIAKVVLLLPPLAERHPGFGRGVAALGSLLAR
                     SGAHLAVYAQSPHGAAAKSVAARLKARGQTQVVEIESWKSAPAKLRAAASGQTAFAIF
                     CSRPGGPAWHPAVEKLPHMLAEEFPDAPLLLFYLPDEAAGGGPLPAAEEEAAAPRDLF
                     EDALKGGRVLVAMPETAVADGVRELLRRSFEGDRRLLGRLSALFTEMAQKAPIELEPG
                     VVLLHAHVPEVEEPLLFFGARPQGFRILALEEPARILVVLCAPEGQLPEAHLAVLGEI
                     ARLFKER"
     gene            complement(2476..3579)
                     /locus_tag="PLG14_06295"
     CDS             complement(2476..3579)
                     /locus_tag="PLG14_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOX48783.1"
                     /translation="MPTLRNFLEKINISARIAFEEPMSRHTSFRIGGPAEAYVAPRDR
                     ESLLKLLGAARAEGIPVFVLGGGANILVGDRGIRGLVLDTGLLAAVGIVEEEGSGDAG
                     APAGAGEAGAGAAGGRAAAAGEGGGSPRGVLLAAEAGLSVDRLCEEAQRRGLAGLEDF
                     YGLPGSVGGAVYMNARCYEVEMADRLAWVEAAKAGTGADQGADLGAGSGGGAGAGVAG
                     TAVERRALAPAEWAYKRSPYQAGGPAAGALVLRAAFRLLPGDPAAIEAAMLARRADRE
                     AKGHYRLPSAGSVFKNDRAIGRPTGKLLDELGFRGRRVGGALVSEWHANIFVNSGGAT
                     AADMRALIESAQREVRARLGLEIEPEVVFVGDF"
     gene            3661..5088
                     /gene="cysS"
                     /locus_tag="PLG14_06300"
     CDS             3661..5088
                     /gene="cysS"
                     /locus_tag="PLG14_06300"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOX48784.1"
                     /translation="MPTLRVYNTLGRELQEFRPLVPGKVGFYGCGPTVYNYAHIGNLR
                     AYVTHDTVSRTLEYLGYEVRHVMNITDVGHLSDDADSGEDKMVKSAHERGKTVYEIAD
                     FYTKAFFEDLDRMNIERPTVVCKATEHIAEMVALIRRIEEKGFTYSAGGNLYFDVTRF
                     PKYGELALLKLEDLKAGARIEVDPNKRNPADFALWFTKSKFEGQAMVWDSPWGRGYPG
                     WHIECSAMSMKYLGEQFDIHAGGIDHIPIHHTNEIAQSEAATGKKWVNYWIHNEFLVL
                     DKGKMSKSGGGFLTLQTLIDKGYEPLDYRYFLLGAHYRSQLQFSFEGLDSAKAARKSL
                     ADRILALRAKAASPAPLSALGAKGKSFLDAFGEALADDFATPRALAALWGALRDPELP
                     PAEALACAFDMDRVLGLGLASIEKAEAAAVDPDLAAWVEGLVAERAAAKKAKDFARAD
                     AIRAELKARGVVLEDGPAGTSWKLA"
     gene            5127..5660
                     /locus_tag="PLG14_06305"
     CDS             5127..5660
                     /locus_tag="PLG14_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX48785.1"
                     /translation="MFSNDGSPDELELRRLYDAAAPMLVRVAYRIAGTEEAAEDIVHE
                     AFIRLLEKRLPFATLDDAKFWLIRVVKNGALNYAKRKGREARAYERWWRGERAESEAA
                     DADILRGESEAEVKLLLERLPEKLKSVLVLKEYGGMSYKEIGRTLGITEGNVKVRAFR
                     AREALLAMIDEEAKHVP"
     gene            5650..6375
                     /locus_tag="PLG14_06310"
     CDS             5650..6375
                     /locus_tag="PLG14_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOX48786.1"
                     /translation="MCPDRGLLSAYVDGEVPSPWKERLAAHLSECPSCAAQAARFGKL
                     SSDLRRPLPFDEEAVAARVAARVAARLGPRLESAPATRPARTRPSASAGGHGLWSRRV
                     ALPLPLAVAAALVLVFTAGLATGFFKPGRPSVQSLAAAEMAPNRAEPASMEALIRYLE
                     SQDAQVNLTIQLPAGKTFDSGGKPLIVKAADYVPLAPAVPAESSSSAAPAGAPAAVSS
                     ESGPAEGGPAASGSAGTGQGGSE"
     gene            6372..6962
                     /locus_tag="PLG14_06315"
     CDS             6372..6962
                     /locus_tag="PLG14_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48787.1"
                     /translation="MSRAPATRAPRGAAIARSFGARSFGAALVYAAALSLAARLEAQE
                     QPLPELFKDKALSVSIQASVVEGPQSVAWESASLRYTVPGNPVGVKLVGSNVVILVQI
                     TPFEDGKGGLVLVSQGQVWVRRGEGDLSYRTTLDSVSVRYGEPVHFFPLGRDPGGKAP
                     IRIEISVDRYRGAPPSGAPSPVSIPAPPAESAKPKK"
     gene            6959..8644
                     /locus_tag="PLG14_06320"
     CDS             6959..8644
                     /locus_tag="PLG14_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOX48788.1"
                     /translation="MKAEPKTPRRSRKALRLALRLSLATLGAAALSAGGWLAYGRWAS
                     PRIERLSPVVAEPGGLIRVEGRNFGAARGESRVEVDGVAPTASSYLSWSASEISLRLP
                     ATADSGLVYVVTRRGRSNPKLFMNRARLPVAARGESAGRSGPFLSSLSAEKGPVGSLL
                     VLSGYNFGANREGGQVLFSWSPEAQALHSGDRIDPTTVAATDSDSSYELWSDKEIRVR
                     VPDGASSGAVVVSTASGRSNGLFFAQAEAPGTKRYLDRRSYSVGYSVSVNKVKASGPS
                     ELYLWVPKPVESSAQRVAKVLAQDPEPLVPDYKGAALYRFKDLQSGQEIAVSQSFLVQ
                     TWEVETVVEPDKIQKPASLPPLYQAALAADELVPAKAPEIQALAKRIVQGERNPWRAA
                     RLIHEYLTRNLAWTDSKTALKPLEALAAKTADSSSYAVIAAALLRAAGVPAQPVTGYL
                     IDPSRRAARHAWVEFYIYGLGWVPMDPILASGARPGGFDAAFTDPGRYFGGMDNHRIS
                     FSRGYAALAPMAPSGRRASPSRPMAYQSFYEEASGALEAYSSFWGEIEVTGLY"
BASE COUNT         1277 a         3263 c         3113 g         1100 t
ORIGIN      
        1 ctccgctcct tgaagaggcg ggcgatctcc ccgaggacgg cgagatgggc ctcgggaagc
       61 tggccctccg gggcgcagag gacgacgagt atgcgggcgg gctcctccag ggccaggatg
      121 cggaagccct gcggcctcgc cccgaagaag aggaggggct cctcgacctc ggggacgtgg
      181 gcgtggagga ggacgacgcc gggctcgagc tcgatgggag ccttctgcgc catctccgtg
      241 aagagggcgg agaggcgccc gaggagcctc cggtcgccct cgaaggaccg cctgaggagc
      301 tcgcggaccc cgtcggccac ggcggtttcg ggcatcgcga ccaggacgcg gccgcccttg
      361 agggcgtcct cgaagaggtc gcggggcgcc gcggcctcct cctcggcggc gggcaggggg
      421 ccgcccccgg cggcctcgtc ggggaggtag aagaggagga ggggggcgtc ggggaactcc
      481 tcggcgagca tgtgcgggag cttctccacg gccggatgcc aggcggggcc gccgggcctg
      541 gagcagaaga tggcgaaggc cgtctggccg gaggcggcgg ccctgagctt ggccggggcg
      601 gacttccagg actcgatctc cacgacctgg gtctgtcccc gggccttgag cctcgcggcc
      661 acggacttgg ccgccgcgcc gtgcggggac tgggcgtaga ccgcgaggtg ggcgccgctc
      721 cgcgcgagga gggagcccag ggccgcgacg ccccgcccga agccgggatg ccgctcggcc
      781 agcggcggga ggaggaggac gaccttcgcg atgcccttga gcggccgctc cgcgcgggcg
      841 accacgacga gctcgcggcc tccctggacg acgcggtcga tgacgctgcc gaagaaggcg
      901 ctggagagcc tgggggcgcg gttccagccc acgacgagga tccgcgcgcg gatctcgtcc
      961 gcggcctgga ggatgccctc gggcacgttg acgcagagcc gggtcgcggg cttcacgggc
     1021 acgcccgcgc gcgtggccag gaccatcgcc ttggccagga ggttctccgc cttcacgagc
     1081 tccgcctcgg cgtcgcccga gtcggccgcg acggcgaggg gatggacggc ctcctcctcg
     1141 tcggggccgc gaaggaggaa ggccaggtcg acgaggggct ggagggtcgc ggggttggag
     1201 agggcgacca tcacgcgctc gggctcctgc cccgagcccg cggggatgga gcccgagcgc
     1261 agggcgagcc tggcgcccgc ggccttggcc gcgacgggcc ccacgacgcc ggccgcgacc
     1321 acgacgagga gggctccgcc gaagagctgc ccgtccacga ggccgaggcg gagaccggcg
     1381 aggaggatgc cgagggcggc cgcgccctgg ttgagggaga ggccgaagcc gaaggcggcc
     1441 tcctcgccgg tgaagcggag gatgggcagc tcgaggaggg aggagagggc cctggccgcg
     1501 aggaggaggc cgaaggcggc cagggccgcg aggagggcgc cgggagcggc ggccaggccg
     1561 gggaggtcgg cgttcatgcc cgcgtagacg aggaagaaag gcaggaagaa ggcgccgccc
     1621 gcgaagccga gcttggccga gaggtccgag gactcgggga cgaagcggcg gaggaggagg
     1681 ccggcgaaga aggcgccgac cgcggagggc agacccgagc gctcggccgc gaaggcgcag
     1741 aggaaggcgg tcgcgaggac gaacacgaac tcgacgcttc cgtcgaccct gacgcgcttg
     1801 aagaaggcgg aggcgaggag gggcaggagg agccagagga gggcggcggc gccgaggccc
     1861 aggccgaggc ggaggggcca gtcggcgccg aagggcgagc cggcgtgggc cgcgcccgcg
     1921 gcggccaggg cggcgacggc gagggtctcg gagaggctcg cgcctccggc cgcggccagg
     1981 gccgagcggg agcgggccag gccgagcttg gcggcggcgg ggtagggctt cgcggacctg
     2041 gccgcgaagg ccgcccccac gaggacggag gagaggaggc tctggccgaa gaggaggctc
     2101 gcggcggcgg cgcccgagcc gaaggggatg gcgaaggaga ggacggccgc gagcgccgag
     2161 ggcgcccggc ccttcctgag cgggacgaag tcgaggcccg cgccagcgag gaggaaggcg
     2221 tacaggagcc ctatctcccc gaggagcccg acggccctgc cctcgcccgg gaggccgagg
     2281 ccgaaggggc cgatgagggc ccccgcgagg gccagggcgg cgaggctggg gacgcggagc
     2341 ttctccgcgg cgatggggac gaggagggtg gcgaggagga cgagggcgac ggtccccacg
     2401 gtgtcggcga agggcatagt cagctaaatg taaggccttc aggccgcggc cgcaagccga
     2461 aagcccgcga ttcccctaga agtcgcccac gaagaccacc tcgggctcga tctcgaggcc
     2521 gaggcgggcg cggacctcgc gttgggcgct ctcgatgagg gcccgcatgt ccgcggccgt
     2581 ggccccgccc gagttgacga agatgttggc gtgccactcc gacacgaggg ccccgcccac
     2641 cctgcgtccc cggaagccga gctcgtccag gagcttgccc gtgggcctgc cgatggcccg
     2701 gtcgttcttg aagaccgagc ccgcggaggg gaggcggtag tgccccttcg cctcgcggtc
     2761 cgcgcggcgg gcgagcatcg cggcctcgat cgcggccgga tcgcctggta ggaggcggaa
     2821 ggccgcccgg aggacgaggg ctcccgcggc cggcccgccg gcctggtagg gcgagcgctt
     2881 gtaggcccac tccgcggggg cgagggcgcg ccgctcgacg gctgtccccg cgacgcccgc
     2941 tcccgcgcct ccgcccgagc ccgcgccaag atccgcgcct tggtccgcgc ccgtgccagc
     3001 cttcgcggcc tcgacccagg ccaggcggtc ggccatctcc acctcgtagc agcgcgcgtt
     3061 catgtagacg gcgccgccca cgctgcccgg cagtccgtag aagtcctcga gccccgcgag
     3121 gccgcgcctt tgggcctcct cgcagaggcg gtcgacgctg aggccggcct cggccgcgag
     3181 gaggactccc cgcggggagc cgcccccttc gcccgcggcc gccgcccgcc cgcccgccgc
     3241 gccagcgccg gcctcgcccg cgcccgccgg cgcgccagcg tcccccgagc cctcctcctc
     3301 cacgatcccc accgcggcga ggaggcccgt gtcgaggacg aggccgcgga tgcccctgtc
     3361 gccgaccagg atgttcgcgc cgccgcccag gacgaagacc gggatgccct cggcccgcgc
     3421 ggccccgagg agcttgagga gggattccct gtccctcggg gcgacgtagg cctcggccgg
     3481 gccgcctatc ctgaagctcg tgtgccgcga catcggctcc tcgaaggcaa tccgcgcgct
     3541 tatattgatt ttttcgagga agttccttag tgttggcatg agccgatcat acccggcggg
     3601 gcgaagggag cgcaagccgc gcggccttcc ccgaccgggc cattccgcca ggaggaagcg
     3661 atgccgacgc tccgcgtgta caataccctg gggcgcgagc tccaggagtt ccgccccctc
     3721 gtccccggca aggtcggctt ctacggctgc ggccccacgg tctacaacta cgcccacatc
     3781 ggcaacctcc gcgcctacgt cacgcacgac acggtctcga ggaccctcga gtacctgggc
     3841 tacgaggtca ggcacgtgat gaacatcacg gacgtcggcc acctctccga cgacgcggac
     3901 tcgggcgagg acaagatggt gaagagcgcc cacgagcggg gcaagacggt ctacgagata
     3961 gccgacttct acacgaaggc cttcttcgag gacctggacc gcatgaacat cgagcggccc
     4021 acggtcgtct gcaaggccac ggagcacatc gccgagatgg tcgccctcat ccggcgcatc
     4081 gaggagaagg gcttcaccta ctccgcgggc ggcaacctgt acttcgacgt gaccaggttc
     4141 cccaagtacg gggagctcgc cctcctcaag ctcgaggatc tcaaggctgg cgcccgcatc
     4201 gaggtcgatc cgaacaagcg gaaccccgcg gacttcgccc tctggttcac caagtccaag
     4261 ttcgagggcc aggccatggt ctgggacagc ccctggggcc gcggctatcc cggctggcac
     4321 atcgagtgct ccgcgatgag catgaagtac ctcggggagc agttcgacat ccacgcgggc
     4381 ggcatcgacc atatccccat ccaccacacg aacgagattg cccagtctga ggccgcgacg
     4441 ggcaagaagt gggtcaacta ctggatccac aacgagttcc tcgtcctgga caagggcaag
     4501 atgtccaagt cgggcggcgg cttcctcacc ctgcagaccc tcatcgacaa gggctacgag
     4561 cccctcgact accgctactt cctcctcggg gcccactacc gctcccagct ccagttctcc
     4621 ttcgagggcc tcgactcggc gaaggccgcg cgcaagtccc tcgccgacag gatcctggcc
     4681 ctgagggcca aggccgcctc tcccgccccc ctttccgccc tgggcgcgaa ggggaagtcc
     4741 ttcctcgacg ccttcggcga ggccctcgcc gacgacttcg ccaccccccg cgccctggcc
     4801 gccctctggg gcgccctccg cgatcccgag ctcccgcccg cggaggccct cgcctgcgcc
     4861 ttcgacatgg accgcgtcct cggcctcggg ctcgcctcga tagagaaggc cgaggccgcc
     4921 gccgtcgacc ccgacctcgc ggcctgggtg gaaggcctcg tcgccgagcg cgccgcggcc
     4981 aagaaggcca aggacttcgc ccgcgcggac gcgatccgcg ccgagctcaa ggcccgcggc
     5041 gtcgtcctcg aggacggccc cgccgggacc tcctggaagc tcgcctaggg cctccgggcc
     5101 cggcgcgtaa cctggacggg gatgggatgt tttctaacga cggctctccc gacgagctcg
     5161 agctccggcg cctctacgac gccgcggctc ccatgctcgt cagggtggcc taccgcatcg
     5221 cgggcacgga ggaggcggcc gaggacatcg tccacgaggc cttcatccgc ctcctcgaga
     5281 agcgcctgcc cttcgcgacc ctggacgacg ccaagttctg gctcatcagg gtcgtgaaga
     5341 acggcgccct caactacgcg aagcgcaagg gccgcgaggc gcgcgcctac gagcgctggt
     5401 ggcggggcga gcgcgcggag agcgaggcgg cggacgcgga catcctgcgg ggcgagtccg
     5461 aggcggaggt caagctcctc ctcgagcggc tcccggagaa gctcaagtcg gtcctcgtgc
     5521 ttaaggagta cggcgggatg agttacaagg agatcggacg gaccctcggc atcaccgagg
     5581 gcaacgtgaa ggtccgcgct ttccgcgccc gcgaagccct gctcgccatg atcgacgagg
     5641 aagccaagca tgtgccctga ccgcggcctt ctttccgcct acgtcgacgg cgaggtcccc
     5701 tctccctgga aggagaggct cgccgcccat ctttccgagt gcccctcctg cgcggcccag
     5761 gccgcccgct tcggcaagct ctcaagcgac ctgcgccggc ccctgccctt cgacgaggag
     5821 gccgtggccg cgagggtcgc cgcccgcgtg gcggccaggc tcggtcccag gctcgagtcc
     5881 gcgcccgcga ctcggcccgc tcggacgcgg ccctcggcct ccgccggcgg ccacggcctg
     5941 tggagccggc gcgtcgccct ccctctcccc ctcgccgtgg ccgccgccct ggtcctcgtc
     6001 ttcaccgccg gcctcgcgac gggcttcttc aagcccggca ggccctccgt gcagagcctc
     6061 gccgccgccg agatggcccc gaaccgcgcc gagcccgcga gcatggaggc cttgatccgc
     6121 tacctcgagt cccaggacgc gcaggtgaac ctcacgatcc agctgcccgc gggcaagacc
     6181 ttcgattccg gcgggaagcc cctcatcgtg aaggccgcgg actacgtgcc cctcgctccc
     6241 gctgtccccg ccgagtcctc ttcctccgcg gcccccgccg gagctcccgc cgcagtttcc
     6301 tcggaaagcg gcccggccga gggaggcccg gccgcaagcg gctcggccgg aaccggccaa
     6361 ggcggctcgg agtgagccgc gcccccgcga cccgcgcccc gcgcggcgcc gcgatcgccc
     6421 gctccttcgg ggcccgctcc ttcggcgctg ccctggtcta cgcggccgcc ttgagcctcg
     6481 ccgcccgcct cgaggcgcag gagcagcccc tccccgagct cttcaaggac aaggccctct
     6541 ccgtctccat ccaggcgagc gtggtcgagg ggccgcagtc ggtcgcctgg gagtcggcga
     6601 gcctccgcta taccgtcccc ggcaaccccg tcggcgtgaa gctcgtgggc tccaacgtcg
     6661 tcatcctcgt gcagataacg cccttcgagg acggcaaggg cggcctcgtc ctcgtgtccc
     6721 agggccaggt ctgggtgcgc cggggcgagg gcgacctctc ctaccggacg accctggact
     6781 ccgtgtccgt ccgctacggc gagcccgtcc actttttccc cctgggccgc gatccggggg
     6841 gcaaggcgcc catcaggata gagatctccg tcgaccgcta ccgcggcgcc cccccctcag
     6901 gcgccccgtc cccggtctcg atcccggcgc cgcccgcgga aagcgcgaag cccaagaaat
     6961 gaaggcagag cccaagaccc ccaggcggag ccgcaaggcc ctccgcctgg ccctccgcct
     7021 gtcgctggcc accctcggcg ccgccgccct gtcggccggc ggctggctcg cctacggccg
     7081 ctgggccagc ccgcgcatcg agcgcctctc tcccgtcgtc gccgagcccg gcggcctcat
     7141 cagggtcgag ggcaggaact tcggcgccgc ccggggcgag tcgcgggtgg aggtggacgg
     7201 cgtcgcgccg accgcctcct cctacctctc ctggagcgcc tcggagatct ccctccgcct
     7261 tcccgcgacc gccgactccg gcctcgtcta cgtcgtgacc aggcgcggaa ggagcaaccc
     7321 gaagctcttc atgaaccgcg ccaggctgcc ggtcgccgcc aggggcgaga gcgcggggcg
     7381 gagcgggccc ttcctctcct ccctctccgc ggagaagggg ccggtcggct ccctcctcgt
     7441 actctcgggc tacaatttcg gggccaaccg cgagggcggc caggtcctct tctcctggag
     7501 ccccgaggcc caggccctcc attcaggcga ccgcatcgat cccacgacgg tcgcggccac
     7561 ggactcggac tcgagctacg agctctggtc cgacaaggag atacgcgtcc gcgtccccga
     7621 cggggcctcc tcaggggccg tcgtcgtgag cacggcctcc ggccgcagca acggcctctt
     7681 cttcgcccag gccgaggcgc cgggcacgaa acgctacctg gacaggcgaa gctactccgt
     7741 cggctactcc gtgagcgtga acaaggtgaa ggcctcgggc ccgagcgagc tctatctctg
     7801 ggtcccgaag cccgtcgagt cctccgccca gcgcgtcgcg aaggtgctgg cccaggatcc
     7861 cgagcccctc gtgcccgact acaagggcgc cgccctctac cgcttcaagg accttcagtc
     7921 cggccaggag atcgcagtct cccagtcctt cctcgtccag acctgggagg tggagaccgt
     7981 cgtcgagccc gacaagatcc agaagcccgc ttcccttccg cccctctacc aggcggccct
     8041 ggccgccgac gagctcgtcc cggccaaggc ccccgagatc caggccctcg cgaagcggat
     8101 cgtccagggc gagaggaatc cctggcgggc cgcccgcctc atccacgagt accttacgcg
     8161 caacctggcc tggaccgact cgaagacggc cctgaagccc ctggaggccc tggccgccaa
     8221 gacggccgac tcctcctcct acgccgtcat cgcggcggcc ctcctccggg cggcgggcgt
     8281 gcccgcccag cccgtgacgg gctacctcat cgacccgagc cggcgcgcgg cgcgccacgc
     8341 ctgggtggaa ttctacatct acggcctagg ctgggtgcct atggacccca tcctggcctc
     8401 gggcgcgcgg ccgggcggct tcgacgcggc cttcactgac ccgggccggt acttcggcgg
     8461 catggacaac caccgcatct ccttctcccg gggctacgcg gccctggcgc ccatggcccc
     8521 gagcggcagg cgcgcctccc cctctaggcc catggcctac cagagcttct acgaggaggc
     8581 ctcgggcgcc ctcgaggcct actcctcctt ctggggcgag atcgaggtca cgggcctcta
     8641 ctgacggcag gccgcgcccc gacggccgcc cgtgccccga cggccggccg cgccccgacg
     8701 gccggccgcg cctcccgttc acgcttcgcg aaaagcgctg tataatcgct ctc
//
LOCUS       DAOOSJ010000100         8717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5114_length_8717_cov_5.554722, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000100 DAOOSJ010000000
VERSION     DAOOSJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_8717_cov_5.554722"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..3340)
                     /locus_tag="PLG14_06325"
     CDS             complement(80..3340)
                     /locus_tag="PLG14_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX48789.1"
                     /translation="MADRDNLKAGERRTATILFSDMKGFTALSERMDPEEMDALMTRV
                     FGLFEEIIRSHGGSVEKYIGDALVAVFGVPELHEDDPSRAIHAALEFLARIREENACL
                     VGRDLCLSFRTGIHTGLVTTGRRGEFDVVTGHAMSVAQRLESAAPLDAILVSESAKEK
                     AEEDFEFGPAQDIEAKGKTEPIRAFAVRSETTSRLRDRGPFVGRREALDELLKAYIRN
                     RYDEVSGFYVQGEAGIGKTRLVQALVEKIRLFPDFKTPVLAARAQKYRPGGFAVITDI
                     VLGYLGLKPGAKPEEARKALLSLPGAGPEAAERFASLLSATEPDKPDPNAIAALYGIF
                     GAILDRHSGDLFPILVVIDNAAFMDRLSREFFQYLFKKGSVKPFFVLTGREFPPELRK
                     AFQGLKPLKLGPLAPEESEALVKAHWPEAGEEALARILEASLGNPLFLREYAAYAAKH
                     KDVSSLPATVQNIFLASLERYPPEWRDLAKRLSVFVHSFTEDDARSLERATEGDPSIV
                     APALARFVQDGLLSTQREYYLFRVDVFKKALYASLLNHNKRILHALVADILLAQDKPH
                     RIRLIYHLVRSERYAEAVKAMQEDPNRNYSYEYLPYIDILYRRLRKDDKAGVRLLITK
                     AALLFNGGKIEESEEVLKRILRIAVAKKDDNLMGYAYHQICAYNAMSYSFQKAIFTGQ
                     KALYYYRRSDIHARSVQNVLRTMVLAQAQRGDIEEARRLVAQCEGVPGGDRFEAAEAR
                     AEFHLLSCDYDKALAVVEKSLAVMPEDRSASLFFAYDLKIKILWQLCDFQGLKEAARR
                     LLALGPLSESALSQANAMLGLASLYYGEREAAADRFVQAEFYSGQIRNDFERLDALRT
                     LALCRYLAGEPKKADETALEALTIGLRHSCYGPTFTLLTLLVESSMERGREERARFFL
                     AEASYLFTTGLLLPSKDLVVYYYYAAKLLSPRDAERHMAVAFRLFEEEKARIGDPELV
                     ANFLSIRSYGRIQRELEGLGEVPSTLLKPERLKRADLAAAEAELDGACYDDEEAEVAE
                     AAGASGASDAAASDASAIISGAGAAADGTSAEAGPAGTVPSAEAEAEAGAARGAKA"
     gene            complement(3390..4727)
                     /locus_tag="PLG14_06330"
     CDS             complement(3390..4727)
                     /locus_tag="PLG14_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48790.1"
                     /translation="MSSKPAPRKGPAPSPGAVVPSRSAYGPQPAPRAAGRRPGQKPPA
                     SLSLPEDAAPASKPAKKRRPRAKGGEARLRLVEVVFFHHYDIGRSADLKKIAAIIPAH
                     DDLGLVKRRDTPASLQLPRPLVVQLGEGECVDAGDFECVSAQGKIYDDAAITIMMRVK
                     IRVPLRELHTIRERRITSGGREITIGDYAEENFRRLFEAIKPAVSGIPEGSDFDRETY
                     AAYCVIDGEEWPGDLIASDREYIAALLSGEDPNTQLHEAQVAATIGRSFSYRRSDLAI
                     FDLDRCFIVDPSRDYEDLLLIIEHANYQLLELRVLDALLDRWLEEAERDMRLMYSGDK
                     KTRRKLGRSVAEKSAHIQALRVDALFILENLENSSKIIGDYYLGQIYDRLCAIFNTEG
                     WKWSVERRLEALQTVYDMLKNDMNERKILVLEVTFIAVCVILPLIQIFQVMIT"
     gene            complement(4724..5776)
                     /locus_tag="PLG14_06335"
     CDS             complement(4724..5776)
                     /locus_tag="PLG14_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOX48791.1"
                     /translation="MKFKLKLPKLPKPSEIKLPLDLSKLESIDREHYKIAAWSLGALV
                     VLMLVAALAAFFLSLRGTEETMVPDVKGLELASALVKLQDKELYPRIALRYTDDPKDR
                     GRIVEQKPPAGAIVKAGRRVQLAVSRGPVVDRMENYVGQDLNEVKIHLQTLFASSRPL
                     VTVKEPPVYVFDKSPAGTGLEQKPLPDTEISGPVPLELVVSRGPEKAQILVPDLKGLG
                     IAEALLQVEKANVATEFSLRKPDGREKPGVVASQTPAPGSLIPANARLSATLTLPAAE
                     KGMVAGLFARELPEYPYPLRLSLEAQKPTGERLPLLRMNHPGGKFSAPYFLPEGSVLV
                     LSVLDREVARSEVKAP"
     gene            complement(5773..6771)
                     /gene="fmt"
                     /locus_tag="PLG14_06340"
     CDS             complement(5773..6771)
                     /gene="fmt"
                     /locus_tag="PLG14_06340"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOX48792.1"
                     /translation="MRILFAGSPEIALPALRRAAAEHELVGVLTNPDAQKGRGLSAAR
                     TPVAQAAEALGVPVLAPERLGPEAREAAAALGAELLVSFAYGRIFGPKFLALFPRGGL
                     NVHPSLLPRWRGCAPIPAAILNRDPETGVAVQRLALAMDEGELLGVERIPLSGRETTE
                     SLSALAAEVGAGLLSRVLREMAEGRETARHQEGEASYCSMLRKEDGLLDFGAPALDLD
                     AKARAFHPWPGAYAFLRGQRLAVLETEPYPGEAWAGPVPAGAAGPGGAPRPGAILGLD
                     KTRGIMVQTGEGLLALRRLQLQHKKALPFREFANGIRDLAGAELGTKAEEEAGSRP"
     gene            complement(6776..7264)
                     /gene="def"
                     /locus_tag="PLG14_06345"
     CDS             complement(6776..7264)
                     /gene="def"
                     /locus_tag="PLG14_06345"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOX48793.1"
                     /translation="MLDIITYGSEALTKKAEPVKEFGPELAQFIEELYEAMKRGRGVG
                     LAAPQVDRLVRVFVTGADGDKLRAFVNPEIVMTSPEEVDFEEGCLSLPGLYTMVKRPE
                     SIKIQAYNEKGRPFTLEASGFLARVILHEYDHLNGLLFIDRLSPVRRERALQQYLKKV
                     RM"
     gene            complement(7426..7938)
                     /locus_tag="PLG14_06350"
     CDS             complement(7426..7938)
                     /locus_tag="PLG14_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOX48794.1"
                     /translation="MLRSSVFRRRASFFVPAAVLVLAAFMPLSGCGGSRAAAQGAPQG
                     PTKPNPTLKTALLKAGGASILAELALTADEREKGLMFRKELPEGKGMLFVFETDQRLA
                     FWMKNTTIPLSLAYLSSDGTIREIRDLEPLSLAPVQAERSVRYALEAPRGWFERAGLK
                     VGDRFELPRF"
     gene            complement(8119..8331)
                     /gene="rpsU"
                     /locus_tag="PLG14_06355"
     CDS             complement(8119..8331)
                     /gene="rpsU"
                     /locus_tag="PLG14_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOX48795.1"
                     /translation="MKQIIVDESEPLEKAIKRFKRMVEKEGIIREWKKREFFEKPSTI
                     KNRKKKALARKLLKKARKPRDSKGGY"
     gene            complement(8337..>8717)
                     /locus_tag="PLG14_06360"
     CDS             complement(8337..>8717)
                     /locus_tag="PLG14_06360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX48796.1"
                     /translation="GGSESSRHLGVDFAVVKGTPVRAAARGRVVFAAERIVTGTTVVI
                     EHLPGLYSIYMHLSSASVAAGRLVERGEVVALSGNSGLSTGPHLHWELRALGRSVDPE
                     AWVKAAPLDKARLIAIMDAAIEGR"
BASE COUNT         1240 a         2789 c         3320 g         1368 t
ORIGIN      
        1 cgctcccgcc tcgcgccgcc agagagccag tcctccacca gggagagctc ctcccagagt
       61 tccttccgct cgtccgggct catgccttgg ctccccgcgc cgcgccggct tctgcctcgg
      121 cctccgcgct cggcaccgta ccggcggggc cggcctccgc ggaagtcccg tccgccgcgg
      181 cgccagcgcc ggagatgatt gcgctggcgt cggaagcggc cgcgtcggag gcgcccgaag
      241 cgcctgcggc ctccgccacc tcggcttcct cgtcgtcgta gcaggcgccg tccagctcgg
      301 cctccgctgc cgcgaggtcg gctcgcttaa gccgctcggg cttgaggagg gtggagggca
      361 cctcgcccag gccctcgagc tcccgctgga tccggccgta ggagcggatg gagaggaagt
      421 tggcgacgag ctcggggtcg ccgatgcgcg ccttctcctc ctcgaagagg cggaaggcca
      481 cggccatgtg ccgctcggcg tcgcggggcg agaggagctt cgccgcgtag tagtagtaga
      541 ccacgaggtc cttggagggg aggaggaggc ccgtcgtgaa gaggtaggag gcctccgcca
      601 ggaaaaaccg cgcgcgctcc tcgcggcccc gctccatcga gctctccacg aggagggtga
      661 ggagggtgaa ggtcgggccg tagcaggagt gccgcaaccc gatggtcagg gcctccaggg
      721 cggtctcgtc ggccttcttg ggctcgcccg cgaggtagcg gcagagggcc agggtccgca
      781 gggcgtccag gcgctcgaag tcgttgcgga tctgccccga gtagaactcc gcctggacga
      841 agcggtcggc cgccgcctcg cgctcgccgt agtagaggga ggccaggccg agcatcgcgt
      901 tcgcctgcga gagggcggac tccgagaggg ggcccagggc caggaggcgc ctggccgcct
      961 ccttgaggcc ctggaagtcg cagagctgcc agaggatctt gatcttgagg tcgtaggcga
     1021 agaagaggga ggcgctccgg tcctcaggca tcaccgcgag gctcttctcc acgacggcca
     1081 gggccttgtc gtagtcgcag gagaggaggt ggaactccgc ccgcgcctcc gcggcctcga
     1141 agcggtcgcc gccggggacg ccctcgcact gggcgacgag gcggcgcgcc tcctcgatgt
     1201 cgccgcgctg ggcctgggcg agcaccatcg tgcggaggac gttctgcacg ctccgcgcgt
     1261 ggatgtcgga gcggcggtag tagtagagcg ccttctggcc cgtgaagatc gccttctgga
     1321 aggagtagct catcgcgttg taggcgcaga tctggtggta ggcgtagccc atcaggttgt
     1381 cgtccttctt cgcgaccgcg atcctgagga tgcgcttcag gacctcctcg gactcctcga
     1441 tcttcccgcc gttgaagagg agggccgcct tcgtgatgag gaggcggacg cccgccttgt
     1501 cgtccttccg gaggcgccgg tagaggatgt cgatgtaggg caggtactcg tagctgtagt
     1561 tgcggttcgg gtcctcctgc atggccttca ccgcctccgc gtagcgctcg gagcgcacga
     1621 ggtggtagat gagccggatg cggtgcggct tgtcctgcgc gaggaggatg tccgccacca
     1681 gggcgtggag gatgcgcttg ttgtggttga ggagggaggc gtagagggcc ttcttgaaga
     1741 cgtccacgcg gaagaggtag tactcgcgct gggtcgaaag gaggccgtcc tggacgaagc
     1801 gcgccagggc cggggcgacg atggaagggt cgccctccgt cgcccgctcc aggctcctcg
     1861 cgtcgtcctc ggtgaaggag tggacgaaga cggagagccg cttcgcgagg tcgcgccact
     1921 cgggcggata gcgctccagg ctcgcgagga agatgttctg caccgtcgcg ggcagggagg
     1981 agacgtcctt gtgcttggcc gcgtaggccg cgtactcgcg gaggaagagg gggttcccca
     2041 ggctcgcctc caggatccgc gccagggcct cctcgcccgc ctccggccag tgggccttca
     2101 cgagggcctc ggactcctcc ggcgcgaggg ggccgagctt caggggcttg aggccctgga
     2161 aggccttcct gagctcgggc gggaactcgc ggcccgtgag gacgaagaag ggcttcacgc
     2221 tgcccttctt gaagagatac tggaagaact cgcgggagag ccggtccatg aaggccgcgt
     2281 tgtcgatgac gaccaggatg gggaagaggt cgcccgagtg ccggtccagg atggcgccga
     2341 agatgccgta gagggcggct atcgcgttgg ggtcgggctt gtcgggctcc gtcgcggaca
     2401 ggagggaggc gaagcgctcc gcggcctccg ggccggcgcc cggcagggag aggagggcct
     2461 tcctcgcctc ctccggcttg gccccgggct tcaggcccag gtagccgagg acgatgtcgg
     2521 tgatgacggc gaagccgccc ggccggtact tctgcgccct ggcggccagg acgggggtct
     2581 tgaagtcggg gaagaggcgg atcttctcca ccagggcctg gaccaggcgg gtcttgccga
     2641 tgccggcctc gccctggacg tagaagccgg agacctcgtc gtagcggttc cggatgtagg
     2701 ccttgaggag ctcgtccagg gcctcccggc ggcccacgaa gggcccgcgg tcgcggagcc
     2761 gggaggtcgt ctcgctccgg acggcgaagg cgcggatggg ctcggtcttg cccttggcct
     2821 cgatgtcctg ggccgggccg aactcgaagt cctcctccgc cttctccttc gcggactcgg
     2881 agaccaggat ggcgtccagg ggggcggcgg actccaggcg ctgggcgacg ctcatcgcgt
     2941 ggccggtcac cacgtcgaac tcgccgcggc ggcccgtcgt gacgaggccc gtgtggatgc
     3001 ccgtgcggaa ggagaggcag aggtcgcggc cgaccaggca ggcgttctcc tccctgatcc
     3061 gggccaggaa ctccagggcg gcgtggatgg cgcgggaggg gtcgtcctcg tggagctcgg
     3121 gcacgccgaa gacggcgacc agggcgtcgc cgatgtactt ctcgacgctg ccgccgtggc
     3181 tgcggatgat ctcctcgaag aggccgaaga ccctcgtcat gagggcgtcc atctcctcgg
     3241 ggtccatgcg ctccgagagg gcggtgaagc ccttcatgtc cgagaagagg atggtcgcgg
     3301 tccggcgctc gccggccttg aggttgtcgc ggtccgccat tgtcccagga ttgtacccgg
     3361 cacgggcccc cgcgcgcaac gggcggggac taggtgatca tcacctggaa gatctggatg
     3421 agggggagga tgacgcagac ggcgatgaag gtcacctcca ggacgaggat cttccgctcg
     3481 ttcatgtcgt tcttgagcat gtcgtagacc gtctgcaggg cctccaggcg gcgctcgacg
     3541 ctccacttcc agccctcggt gttgaagatc gcgcagaggc ggtcgtagat ctgccccagg
     3601 tagtagtcgc cgatgatctt ggaggagttc tccaggttct cgaggatgaa gagggcgtcc
     3661 acgcgcaggg cctggatgtg ggccgacttc tcggcgacgg agcggcccag cttgcgccgg
     3721 gtcttcttgt cgcccgagta catgaggcgc atgtcgcgct ccgcctcctc cagccagcgg
     3781 tccaggaggg cgtccaggac gcggagctcc aggagctggt agttggcgtg ctcgatgatg
     3841 aggaggaggt cctcgtagtc gcggctcggg tccacgatga agcagcggtc caggtcgaag
     3901 atcgcgaggt cgctcctccg gtaggagaag ctccggccga tggtcgcggc gacctgcgcc
     3961 tcgtggagct gggtgttcgg gtcctcgccg gagaggaggg cggcgatgta ctcgcggtcg
     4021 ctcgcgatga ggtcgccggg ccactcctcg ccgtcgatga cgcagtaggc ggcgtaggtc
     4081 tcgcggtcga agtccgagcc ctccgggatg ccggagaccg cgggcttgat cgcctcgaag
     4141 aggcgccgga aattctcctc ggcgtagtcg ccgatggtga tctcgcgccc gccggaggtg
     4201 atgcgccgct cgcggatcgt gtggagctcg cgcagcggca cgcggatctt cacgcgcatc
     4261 atgatcgtga tcgcggcgtc gtcgtagatc ttgccctggg cggagacgca ctcgaagtcc
     4321 cccgcgtcca cgcactcgcc ctcgccgagc tggacgacga ggggccgcgg cagctggagg
     4381 gaggccggcg tgtcgcggcg cttcacgagc ccgaggtcgt cgtgggcggg gatgatggcg
     4441 gcgatcttct tgaggtcggc gctgcggccg atgtcgtagt ggtggaagaa gacgacctcc
     4501 accaggcgga ggcgggcctc gccccccttg gcgcggggcc gccgcttctt ggctggcttg
     4561 gaggcggggg cggcgtcctc cggcagggac agggaggcgg gaggcttctg gccaggccgg
     4621 cggccggcgg cgcgcggagc gggctggggg ccgtaggcgc tccgcgaggg cacgacggcg
     4681 ccggggctcg gggccgggcc tttccgcgga gcgggcttgg agctcatggt gccttgacct
     4741 ccgacctcgc gacctcgcgg tccaggacgg agaggacgag gaccgagcct tcgggcagga
     4801 agtagggggc gctgaacttg ccgcccgggt ggttcatgcg gaggaggggc aggcgctcgc
     4861 ccgtcggctt ctgggcctcc agggagagcc tcaaggggta gggatactcg gggagctccc
     4921 gggcgaagag gccggcgacc atgcccttct ccgcggcggg cagggtgagg gtggcggaga
     4981 gccgcgcgtt ggcggggatg agggagccgg gggccggggt ctggctcgcg acgacgccgg
     5041 gcttttccct gccgtcgggc ttgcggagcg agaactccgt ggcgacgttg gccttctcca
     5101 cctggaggag ggcctcggcg atgccgagcc ccttcaggtc gggcacgagg atctgggcct
     5161 tctcggggcc gcggctcacc acgagctcca ggggcacggg gccggagatc tccgtgtcgg
     5221 ggaggggctt ctgctccagg ccggtgcccg cgggcgactt gtcgaagaca tagacgggcg
     5281 gctccttcac cgtcacgagg ggacgggagg aggcgaagag ggtctggagg tggatcttca
     5341 cctcgttcag gtcctggccg acgtagttct ccatgcggtc cacgacgggg ccgcggctca
     5401 cggcgagctg gacgcggcgg ccggccttca cgatggcgcc ggctgggggc ttctgctcca
     5461 cgatgcggcc gcggtccttg ggatcgtccg tgtagcggag ggcgatcctg ggatagagct
     5521 ccttgtcctg gagcttcacg agggcggagg cgagctccag gcccttcacg tcggggacca
     5581 tcgtctcctc cgtgccccgc agggagagga agaaggcggc cagggccgcg acgagcatga
     5641 ggacgaccag ggcgccgagg ctccaggccg cgatcttgta gtgctcgcgg tcgatgctct
     5701 cgagcttgga caggtcgagg gggagcttga tttcggaggg cttgggcagt ttgggcagct
     5761 tgagcttgaa cttcatgggc gtgatccggc ttcctcttcg gcctttgtcc cgagctcggc
     5821 gccggccaaa tcgcgtattc cgttggcgaa ctcccggaac gggagggcct tcttgtgctg
     5881 gagctggagg cgcctgaggg ctagtagacc ttctcctgtt tgtaccatta tgcccctcgt
     5941 cttgtctagg ccgagaattg cgcccggccg gggagcgccg ccggggcccg cggcgcccgc
     6001 cgggaccgga ccggcccagg cctcgccggg atagggctcg gtctccagga cggccaggcg
     6061 ctggccgcgg aggaaggcgt aggccccggg ccaggggtgg aaggcccgcg ccttcgcgtc
     6121 caggtccagg gccggggcgc cgaagtcgag gaggccgtcc tccttcctga gcatggagca
     6181 gtagctcgcc tcgccctcct ggtgacgggc cgtctcccgg ccctccgcca tctcgcggag
     6241 cacgcgggag aggaggccgg cgccgacctc cgccgccagg gcggagaggc tctccgtcgt
     6301 ctcgcggccc gaaaggggga tgcgctcgac gcccaggagc tccccctcgt ccatcgcgag
     6361 ggcgaggcgc tggacggcga ccccggtctc gggatcgcgg ttcaggatgg ccgccgggat
     6421 gggcgcgcag ccgcgccagc gcgggaggag gctcgggtgg acgttgaggc cgccccgcgg
     6481 gaagagggcc aggaacttcg ggccgaagat gcgcccgtag gcgaagctca cgaggagctc
     6541 cgcgcccagg gccgcggccg cctcgcgggc ctcggggccc aggcgctctg gcgcgaggac
     6601 cgggacgccg agggcctcgg cggcctgggc gacgggggtg cgggcggccg agaggccgcg
     6661 gcccttctgg gcgtcggggt tcgtgaggac gccgacgagc tcgtgctccg ccgcggcccg
     6721 cctgagcgcg ggcagggcta tctcggggct gcccgcgaag aggatccgca cggtcctaca
     6781 tcctgacctt tttcaggtac tgctggaggg cccgctcgcg gcggacgggg gagaggcggt
     6841 cgatgaagag gaggccgttc aggtggtcgt actcgtggag gatgacccgg gccaggaagc
     6901 cggaggcctc cagggtgaag ggcctgccct tctcgttgta ggcctggatc ttgatggact
     6961 ccgggcgctt caccatcgtg tagaggccgg gcagggagag gcagccctcc tcgaagtcga
     7021 cttcttccgg ggaggtcatg acgatctcgg gattgacgaa ggcgcggagc ttgtcgccgt
     7081 cggcgccggt gacgaagacg cggaccaggc ggtccacctg gggcgcggcc aggcccactc
     7141 ccctgccccg cttcatcgcc tcgtacagct cctcgatgaa ctgggcgagc tcggggccga
     7201 actccttgac cggctcggcc ttcttcgtga gcgcctcgct gccgtaagtg atgatgtcga
     7261 gcacggttcc tccacgtagg tttaaaatag ccacggcacg ccgggacgtc aatgcgcgcg
     7321 cctggggcgg cccggcgccc gcgctcccgg caggcacgac gggcacggcg ggcacggcgg
     7381 cggccgcgga aagccgtgga cagccgcccc gcccggcggc tctgcctaga agcgcggcag
     7441 ctcgaagcgg tcgccgacct tgaggccggc ccgctcgaac cagccccgcg gcgcctccaa
     7501 ggcgtagcgc acggagcgct cggcctggac cggggccagg gagaggggct cgaggtccct
     7561 gatctcgcgg atcgtcccgt cggaagagag gtaggccagg gagaggggga tcgtcgtgtt
     7621 cttcatccag aaagccaggc gctggtccgt ctcgaagacg aagagcatgc cctttccctc
     7681 cgggagctcc ttgcggaaca tgaggccctt ctcccgctcg tcggccgtga gggcgagctc
     7741 ggccaggatg ctcgccccgc ccgccttgag cagggccgtc ttcagggtcg gattgggctt
     7801 cgtcgggccc tgcggagcgc cctgggcggc cgcgcgggag ccgccgcagc ccgacagggg
     7861 catgaaggcc gcgaggacca ggacggccgc cgggacgaag aaagaagccc tccgcctgaa
     7921 tacgctggaa cgaagcatgg agacctcctg tatgcccggc gggccggcgg gccgccgagc
     7981 cgagcctggc tagccggccc aaccgggcgg cgccgcgcca ggccggccgc cccgcgaatc
     8041 gggcgcgccg gcggcaataa aaaagccgta aaggattgac ctttacggcg gattcaccag
     8101 gatttcgatc gaagggatct agtagccgcc cttggagtcc cggggcttgc gcgccttctt
     8161 gaggagcttg cgcgcgaggg ccttcttctt ccggttcttg atcgtggagg gcttctcgaa
     8221 gaattcgcgc ttcttccact cccggatgat gccctccttc tcgaccatgc gcttgaagcg
     8281 cttgatcgcc ttctcgaggg gctcgctctc gtctacgata atctgcttga tgttaatcac
     8341 ctcccttcga tcgcggcgtc cattatcgcg ataaggcgag ctttgtcaag cggcgcggcc
     8401 ttgacccagg cctccgggtc gaccgagcgg cccagggcgc ggagctccca atggaggtgg
     8461 ggcccggtgg agaggcccga gttgccggag agggcgacga cctcgccccg ctccacgagc
     8521 cgcccggccg cgacggaggc cgaggagagg tgcatgtaga tcgagtagag gccgggcagg
     8581 tgctcgatga ccaccgtcgt ccccgtgacg atgcgctccg cggcgaagac cacgcggcct
     8641 ctggccgcgg cccggacggg cgtgcccttg acgacggcga agtcgacgcc caggtggcgg
     8701 ctcgactcgg agccgcc
//
LOCUS       DAOOSJ010000101         8705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5122_length_8705_cov_5.677110, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000101 DAOOSJ010000000
VERSION     DAOOSJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5122_length_8705_cov_5.677110"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLG14_06365"
     CDS             complement(<1..558)
                     /locus_tag="PLG14_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48797.1"
                     /translation="MTKSETTILESKDPAAYIDSSLKSRLSPAAKARLARLWMEKTGY
                     SKEDILRARNRHPYWRKKKMEGSVERTRRRLAAHDYSGGAEVEWTRARIEEFLSLNGK
                     GRDGRYEMKDWQLAEHFGSSIPSIQYMRRKCRKVESLIGARASRAKTLDYLMRAESVL
                     KRGAGAVEALAASEKERAKATLKAPA"
     gene            complement(620..1993)
                     /locus_tag="PLG14_06370"
     CDS             complement(620..1993)
                     /locus_tag="PLG14_06370"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOX48798.1"
                     /translation="MIDLQPLVNKNVPFKFKGAELKLDLSHALFSSFNVDVGTRLLLK
                     AVGRDEVLAGARRVLDAGSGVGVIGVAVAKAFPEARVEARDRDLLACAFTERNGARNK
                     APNLLVRPGLLATGAAVSAAHPDPALPGGGYDFILSNLPAKAGGPVLEAFFKGAASLL
                     APGGRLAVVIVKPLVEAALAWIEGAGLDIVSSERGSGHMAWVLAPRAAAPSQAGAAQV
                     PAAAAPTQSQAAAAPAPPQAGVPGAAQPVPASAPAEGPELDLSGLDLGVYLRRRGRFR
                     LGEASYEASGYWGLPDFDTVGFSPRVAAELAERAIAGTLVREALVVNPGLGHFPLWLA
                     RRAGPARIQAASRDLLSLAATGANLAALAGRRPAYRALDALRLDELPSASLDLLAEFP
                     DAVPEYDWIEPLWDRAFRLLKRGATFLLAATPTELARAEKRRPQGFRLLGDRRRKGQA
                     AMAWRRD"
     gene            complement(2060..3796)
                     /locus_tag="PLG14_06375"
     CDS             complement(2060..3796)
                     /locus_tag="PLG14_06375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOX48799.1"
                     /translation="MVPLYKTKIFRRQFFALLGLSLLFFAAMGGAIILRTRRAVEARE
                     LEHSAAYRDQVGKHLLSWLGERKDDVALLARTMELEAGPEGPAAARLGAFLASKPDFV
                     DAFLVDAQGRVALTKVGPSAQRTYLGDRDYARSALSGKPFVSGYFEARLRKTPIFAVA
                     EPLWRDGRVAGAAVGVISLSRLLGVVDAASLEHLGSAFLVDSRRALVSNPEFAKGLAE
                     AESAAGAGTATAAGAAAGSGQAAALVIDSLAAREVAAGRSGSASYGDASGKKVIGSFG
                     WIEDLRLGLVVELSYERALGPVTGLLRFAAFLALAMLLLLAALSYALSARLVGPIAAL
                     LAAVGAVAEGRFDAPMPASAGNELDQLGELFGRMAGAVRDREERLKESAARDSLTGLY
                     NHGRIIEYLELELRRKRRSGEPVTLAMLDIDHFKAVNDGHGHVAGDAVLRGLAGILQS
                     SAREGDLVGRYGGEEFAVILDSGSPELAAAFCERIRSKVEAAAFAHEGLDLRVTASLG
                     WASSPAGCLQAAGLVALADAALYRAKAEGRNRVRGAVGAAGAAGGGAAAPGSAAEAGC
                     AEEESLQKPPKG"
     gene            complement(3925..4413)
                     /locus_tag="PLG14_06380"
     CDS             complement(3925..4413)
                     /locus_tag="PLG14_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOX48800.1"
                     /translation="MEASNANQYLTFTLDNEQYAIGVAKVREVLEHTKITKLPRTAEF
                     MKGIINLRGAGVPVIDLRLKFGLPETPITKDTSIIVMEVESQEGTVVVGALADAVHEV
                     VEIDENAVEAAPRFGTRLAAEFIRGVGKKDEAFIILLDIDRIFNAEDISALAGSREAE
                     AV"
     gene            complement(4554..5729)
                     /locus_tag="PLG14_06385"
     CDS             complement(4554..5729)
                     /locus_tag="PLG14_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOX48801.1"
                     /translation="MSREAESESRSRALAAALAGAGLSAWGLVSRPALEEACARLPQA
                     VRAKFGVDAAKGAVAAALAYGTGPRESPTWAQGYGGPPLSIARFARADWYAELSSRLR
                     AAAARTRESLAAQGLEPGAARNWRHLANSGLPEKPLALAAGLGRLGRNGLVIVRAEPE
                     GGGVEIGPGVVLGLLLLPFEPCLPVGWPEKRPGLRSDAGREARPGGRLWGQGAALPEA
                     FILGGLCGSCRACVEACPTGALGEDGAFARERCIQHWTAREGALPPAVEAAWGDRLYG
                     CEACLEACPFYRPEAEEGGPGRAADAGFVGAGSADAALIGAAPAAGEARGGAAPSSAA
                     PACFRGALGPSLPAGFFLTESEAAIRERLRGTALGLGWMSVEAFRRSARLALSRRKA"
     gene            complement(5860..7095)
                     /locus_tag="PLG14_06390"
     CDS             complement(5860..7095)
                     /locus_tag="PLG14_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48802.1"
                     /translation="MESPLQLSFVNFKKDSYIIVEGKQNADRFFIIRSGKVRVSKEVE
                     VVEEEGGNVLGPGDFFGVVSTMSNHSHIETAQAVTDCTLISVHRDQFGLLIEKNTPVA
                     MKIIMAFSKRMRYLDEALTRLTLKNSAEADPSHLFRVAEYYARLSLYNQAFYAYYQYL
                     KFCPNGQHVEIARERLNKIKPYSKAVYLDGSTEEFNRFYPKDTMIFSEGMPGSELYII
                     QKGSVKITKISDNNEVLLAVLRPGDIFGEMAILENKPRSASAVAFEDAYLLAVNKANF
                     ERMVQTQPQIVTRLTTLLSERIWFIYKQLANTLISDPLGRMYDALLIQLEKNRVPLKA
                     GASYAFDFGPKELVGMVGLPVGEGNLMVRKLLDNQKLRLADNKLMAVDVTEVEKQTEY
                     YRKMQKIERSRKEGASALR"
     gene            complement(7293..8576)
                     /gene="glyA"
                     /locus_tag="PLG14_06395"
     CDS             complement(7293..8576)
                     /gene="glyA"
                     /locus_tag="PLG14_06395"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOX48803.1"
                     /translation="MSLIEKQDPELWKAMSLEADRQRENLELIASENYTSRAVQEAVG
                     SVLTNKYAEGYPGKRYYGGCEFVDIAENLARDRAKELFGADHANVQPHSGSQANMGVY
                     FAALKPGDTILGMDLAHGGHLTHGAPVSFSGKLYKVVRYGVNRETEKIDYDELAKIAR
                     EAKPRMIVAGASAYARTLDFERFRAIADEVGAYLMVDMAHIAGLVATGLHPNPVKLAD
                     FTTTTTHKTLRGPRGGMILVGRDKENDQGIMNPKGDKTKLWSEIVDGTIFPGIQGGPL
                     CHVVAGKAVAFKEALGPEYKAYMKRVVENAAALARDLAAGGARIVSGGTDNHLMLVDL
                     SPLGITGKEAEKWLDEAHITVNKNGIPFDTKSPFITSGIRVGSPAVTTRGMGLSEMGE
                     IAGYIMEVLKAKGDEKVVAGVKEKVLRLTKRFPLP"
BASE COUNT         1236 a         2843 c         3295 g         1331 t
ORIGIN      
        1 ggccggcgcc ttcagggtgg ccttggcgcg ctccttctcg gaggcggcca gggcctccac
       61 cgcgccggcg ccgcgcttga gcaccgactc ggcccgcatg aggtagtcga gcgtcttcgc
      121 ccgcgaggcc cgggccccga tcagggactc gaccttgcgg cacttgcgcc tcatgtactg
      181 gatcgagggg atgctggagc cgaagtgctc ggcgagctgc cagtccttca tctcgtagcg
      241 gccgtcccgg cccttgccgt tgagggagag gaactcctcg atacgcgccc tggtccactc
      301 gacctcggcg ccgcccgaat agtcgtgcgc ggcgagccgg cgcctcgtgc gctcgacgga
      361 gccctccatc ttcttcttcc gccaataggg atggcggttc ctggcgcgga ggatgtcctc
      421 tttcgaataa cccgtcttct ccatccacag cctggcgagc ctggccttcg cggccgggct
      481 caggcgggac ttgagcgagc tgtcgatgta ggccgcgggg tccttcgact ccaggatggt
      541 cgtctctgac ttggtcatgc ttccatttta cttcgcgcga aaacgcaatg caaatgccgc
      601 ggagaaatat atcttgcggc taatcccggc gccaggccat ggccgcctgg cccttccgcc
      661 gcctgtcccc gaggaggcgg aagccctggg gccgccgctt ctccgcccgc gcgagttccg
      721 tgggcgtcgc cgcgaggagg aaggtggccc ccctcttgag gaggcggaag gcccgatccc
      781 agagcggctc gatccagtcg tactcgggca cggcgtcggg gaactccgcg aggaggtcca
      841 gggaggccga gggaagctcg tcgaggcgca gggcgtcgag ggcgcgatag gcgggccttc
      901 tcccggccag ggcggccagg ttggcgccgg tggcggccag cgagaggagg tcgcgcgagg
      961 cggcctggat ccgcgcgggg ccggcccgcc tcgcgagcca gaggggaaaa tgccccaggc
     1021 cggggttaac gaccagggcc tcgcggacca gggtgccggc gatggcccgc tcggcgagct
     1081 cggcggcgac gcggggcgag aaccccaccg tgtcgaagtc gggcaggccc cagtagccgc
     1141 tcgcctcgta ggaagcctcg cccaggcgga agcggccgcg gcggcggagg tagacgccca
     1201 ggtccaggcc cgagaggtcg agctcggggc cctccgcggg cgcggaggcg gggacgggtt
     1261 gcgcggcgcc cgggacgccg gcttgcggcg gcgccggagc cgcggcggct tgagactgag
     1321 tcggagccgc agcggccgga acctgagccg cgccggcctg gcttggggcc gccgcccgcg
     1381 gggccaggac ccaggccatg tggcccgagc cgcgctcgga ggagacgatg tccaggccgg
     1441 cgccctcgat ccaggccagg gcggcttcga cgaggggctt cacgatgacg acggccaggc
     1501 ggccgcccgg agctagaagg gaggccgcgc ccttgaagaa ggcctccagg acggggccgc
     1561 cggccttcgc ggggaggttc gagaggatga agtcgtagcc gcccccggga agggcggggt
     1621 cgggatgggc ggcggagact gcggcgccgg tggcgaggag tccgggcctg accaggaggt
     1681 tcggggcctt gttcctggcc ccgttccgct cggtgaaggc gcaggccagg aggtcgcggt
     1741 cccgcgcctc gaccctggcc tcggggaagg ccttggcgac agcgacgccg atgacgccga
     1801 cgcccgagcc cgcgtcgagg acccgcctgg ctccggccag gacctcgtcg cgcccgaccg
     1861 ccttgaggag gagcctcgtg cccacgtcca cgttgaagga ggagaagagg gcgtgggaga
     1921 ggtcgagctt gagctcggca cccttgaact tgaagggcac gttcttgttg acgaggggct
     1981 gtaggtcgat cactggcagt cctggcgcaa gggatggtcg gaccggacgg ccttgcgagg
     2041 cgcccgtccg acgtagattc tagcctttcg ggggcttttg taaagattcc tcttcggcac
     2101 agcccgcctc cgcggccgag cctggggcgg cggccccgcc gcccgccgcg ccggcggctc
     2161 cgacggcgcc gcgaacgcgg ttacgcccct ccgccttcgc ccggtacagg gcggcgtcgg
     2221 ccagggcgac gaggccggcg gcctggaggc agccggccgg ggacgaggcc cagccgaggc
     2281 tcgccgtcac gcggaggtcg aggccctcgt gcgcgaaggc ggcggcctcc accttggagc
     2341 ggatgcgctc gcagaaggcg gcggcgagct cgggggagcc cgagtccagg atgacggcga
     2401 actcctcgcc gccgtagcgg cccacgaggt cgccctcccg ggcgctcgac tggaggatgc
     2461 cggccaggcc ccggaggacg gcgtccccgg ccacgtggcc gtggccgtcg ttcaccgcct
     2521 tgaaatggtc gatgtcgagc atcgcgaggg ttacgggctc gccggagcgc cgcttccgcc
     2581 tgagctcgag ctcgaggtac tcgatgatcc tcccgtggtt gtagagcccc gtcaggctgt
     2641 cgcgcgcggc gctctccttg agccgctcct cgcggtcgcg gaccgccccg gccatgcggc
     2701 cgaaaagctc gccgagctgg tccagctcgt tgccggccga ggcgggcatt ggcgcgtcga
     2761 agcgcccctc ggccacggcc cccaccgccg cgaggagggc ggctatcggg ccgacgagcc
     2821 tcgcggagag ggcgtaggag agggccgcga ggaggaggag catagccagg gcgaggaagg
     2881 ccgcgaacct gaggaggccg gtcaccggcc ccagggcgcg ctcgtaggag agctcgacga
     2941 cgaggccgag ccggaggtct tcgatccagc cgaaggagcc gatgaccttc ttcccggagg
     3001 cgtcgccata ggaggccgag cccgaccttc cggcggcgac ctcccgcgcg gccaggctgt
     3061 cgatgacgag ggccgcggcc tggccgcttc ccgccgccgc gccggccgcc gtcgcggtcc
     3121 ccgccccggc cgcggactcc gcttcggcca ggcccttcgc gaactcgggg ttggagacga
     3181 gggcgcggcg cgagtcgacg aggaaggcgg agccgaggtg ctcgagggag gccgcgtcga
     3241 cgacgcccag gagccgggag aggctgatga cgccgacggc cgcgcccgcg acgcgaccgt
     3301 cccgccacag gggctcggcg accgcgaaga tgggggtctt cctgaggcgg gcctcgaagt
     3361 agccgctcac gaagggcttt cccgacaggg ccgagcgcgc gtagtcccgg tcgcccaggt
     3421 aggtgcgctg ggcggagggc ccgaccttgg tcagcgccac cctcccctgg gcgtcgacga
     3481 ggaaggcgtc gacaaagtcg ggcttcgagg ccaggaaggc gccgaggcgg gccgccgccg
     3541 gcccctcggg tccggcctcg agctccatgg tcctcgcgag gagggcgacg tcgtccttcc
     3601 tctccccgag ccaggagagg aggtgcttcc cgacctggtc gcggtaggcg gccgaatgct
     3661 cgagctcgcg ggcctccacg gccctccgcg tgcgcaggat gatggccccg cccatcgcgg
     3721 cgaagaagag cagggaaagc cccaggaggg cgaagaactg tctgcggaat atcttcgtct
     3781 tgtagagcgg aaccatggac tctcccgtcc ccatactaca ggaaagggcc tcaggatggg
     3841 aacaagggcg gcgcggacag gtgcccctaa aacgaggggc cggacgcctt ggcgcccggc
     3901 cccggatgcc gcgcggaagc cggcctagac ggcctcggcc tcgcggctcc cggcgagggc
     3961 ggagatgtcc tcggcgttga agatccggtc tatgtcgagg aggatgatga aagcctcgtc
     4021 cttcttgccg acgccgcgga tgaactcggc cgcgagcctg gtgccgaagc ggggggccgc
     4081 ctccaccgcg ttctcgtcga tctccacgac ctcgtggacg gcgtcggcga gggcgccgac
     4141 gacgaccgtg ccctcctggc tctccacctc catcacgatg atgctcgtgt ccttggtgat
     4201 gggcgtctcg ggcaggccga atttcaggcg gagatcgatg acgggcacgc cggcgccccg
     4261 aaggttgatg atgcccttca tgaactcggc ggttcggggg agtttcgtga tcttggtgtg
     4321 ctcgagaacc tcgcggacct tggcgacacc gatggcgtac tgctcgttgt cgagcgtaaa
     4381 ggtgaggtat tgattcgcgt tcgatgcttc catatctcgc agctcctctt attagtccat
     4441 tctaatcgtg ccggaggccc tcgtcaaatg cccggccggg gatgccgggg aagccggggg
     4501 agccaggctt ggcccctccc ggccaggcca ggccaggccc gaagggccgg cttctaggcc
     4561 ttccgccggg agagggcgag cctggcgctc cgcctgaagg cctcgacgct catccagccc
     4621 aggcccagtg cggtgccccg caggcgctcg cggatggctg cctcgctctc cgtgaggaag
     4681 aagcccgcgg gcaggctggg ccccagggcc ccgcggaagc atgccggagc cgcgctggag
     4741 ggagccgcgc cgccccgcgc ctcgccggcg gcgggagccg cgcctataag ggcggcgtcg
     4801 gcggaacccg cgccgacgaa acccgcgtcg gcggcgcggc caggcccccc ctcctcggcc
     4861 tcggggcggt agaaggggca ggcctccagg caggcttcgc agccgtagag cctgtccccc
     4921 caggccgcct ccacggccgg aggcagggcg ccctccctgg ctgtccaatg ctggatgcaa
     4981 cgctcccgcg cgaaggcgcc gtcctcgccc agggcgcccg tcgggcaggc ctccacgcag
     5041 gcccggcagg agccgcagag gccgcctagg atgaatgcct cgggtagcgc tgccccttgc
     5101 ccccagagcc gccctccggg acgagcctcg cgccccgcat cggaccgcag ccccggacgc
     5161 ttctcgggcc agcctacggg aaggcagggc tcgaagggga ggaggaggag gccgaggacg
     5221 acgcccggcc ctatctcgac gccgccgccc tcgggctcgg cgcggacgat gacgaggccg
     5281 ttgcgtccca ggcggccgag gccggcggcc agggcgaggg gcttctcggg gaggccggaa
     5341 ttggccagat gccgccaatt ccgggccgcg cccggctcga ggccctgggc ggcgaggctt
     5401 tcccgggtcc gggcggcggc ggcgcggaga cggcttgaga gctccgcgta ccaatcggcg
     5461 cgggcgaagc gggcgatgga taggggcggg ccgccatagc cctgggccca ggtgggggac
     5521 tcccgcggcc cggtgccgta ggccagggcc gccgcgaccg ctcccttcgc ggcgtcgacg
     5581 ccgaacttgg cgcggaccgc ctgggggagg cgcgcgcagg cctcctccag ggcggggcgg
     5641 gagacgaggc cccaggcgga gagaccggct ccggccaagg ccgcggcgag ggccctcgac
     5701 ctcgattcgc tctcggcttc gcggctcatg ccgttcgacg cctcccctcg cgccgcccgc
     5761 cggcggcgat gtcgacgcgg ctaatggtaa tagggaaggg gactcggggc gaggttcggc
     5821 gtggtggtgc cagcgggaag ccgccttggg aagaccgccc tagcggaggg ccgaggcgcc
     5881 ttccttgcgg gagcgctcga tcttctgcat cttgcggtag tactcggtct gcttctccac
     5941 ctcggtgacg tcgacggcca tgagcttgtt gtcggcgagg cggagcttct ggttgtcgag
     6001 gagcttccgc accatgaggt tgccctcccc gacgggaagg ccgaccatgc cgacgagctc
     6061 cttggggccg aagtcgaagg cgtaggaggc gccggccttg agggggacgc ggttcttctc
     6121 gagctggatg aggagggcgt cgtacatccg ccccagggga tcggagatca gggtgttggc
     6181 gagctgcttg tagatgaacc agatccgctc ggagaggagg gtcgtgaggc gggtcacgat
     6241 ctggggctgg gtctggacca tgcgctcgaa attggccttg ttgaccgcga ggaggtaggc
     6301 gtcctcgaag gcgaccgcgc tcgcggagcg gggcttgttc tccaggatgg ccatctcgcc
     6361 gaagatgtcg ccgggcctga ggacggcgag gaggacctcg ttgttgtcgg agatcttcgt
     6421 gatcttcacc gagcccttct ggatgatgta gagctcggag ccgggcatgc cctccgagaa
     6481 gatcatcgtg tccttcgggt agaagcggtt gaactcctcg gtggagccgt ccaggtacac
     6541 ggccttggag tagggcttga tcttgttgag ccgctcgcgc gcgatctcga cgtgctggcc
     6601 gttcgggcag aacttgaggt actggtagta ggcgtagaag gcctggttgt agaggctgag
     6661 gcgcgcgtag tactccgcga cgcggaagag gtgggagggg tcggcctccg cggagttctt
     6721 gagggtgagg cgggtcaggg cctcgtcgag gtagcgcatg cgcttggaga aggccatgat
     6781 gatcttcatc gcgacggggg tgttcttctc gatgaggagg ccgaactggt cgcggtggac
     6841 ggagatgagg gtgcagtcgg tgaccgcctg ggcggtctcg atgtgggagt ggttggacat
     6901 cgtggagacg acgccgaaga agtcgcccgg cccgaggacg ttgccgccct cttcctcgac
     6961 gacctcgact tccttcgaga cgcgcacctt tcccgatcga atgatgaaga agcggtcggc
     7021 gttctgcttc ccctcgacga tgatgtacga gtccttcttg aagttgacga acgagagctg
     7081 gagcgggctt tccatcctgt ttcctgaccg tacctcaagg ggccgacttc gcttaagtat
     7141 agagggggtg ctccgaggtg tcaacgcgag cccctccatt gtctatcggc gccataaggg
     7201 gccgcctgta gggccggttc gcccggtaaa acggcgcgga ccggcccgga gcggggcggc
     7261 gggaggcccg caaggcctcc cgcgcgggcc ctttagggca gggggaagcg cttggtgagc
     7321 cggaggacct tctccttgac gcccgcgacg accttctcgt cgcccttggc cttgaggacc
     7381 tccatgatgt agccggcgat ctcccccatc tcggaaaggc ccatgccgcg ggtcgtcacc
     7441 gcggggctgc ccacgcggat gcccgaggtg atgaaggggc tcttcgtgtc gaaggggatg
     7501 ccgttcttgt tgaccgtgat gtgggcctcg tccagccact tctcggcctc cttgcccgtg
     7561 atgcccaggg gcgagaggtc gacgagcatg aggtggttgt ccgtgccgcc ggagacgatg
     7621 cgggcgccgc cggctgcgag gtccctcgcg agggctgccg cgttctcgac gacgcgcttc
     7681 atgtaggcct tgtactcggg gccgagggcc tccttgaagg ccacggcctt gccggcgacg
     7741 acgtggcaga gggggccgcc ctggatgccg gggaagatcg tcccgtcgac gatctcggac
     7801 cagagcttgg tcttgtcgcc cttcgggttc atgatgccct ggtcgttctc cttgtcgcgg
     7861 ccgacgagga tcatgccgcc gcgggggccg cggagggtct tgtgggtcgt cgtggtcgtg
     7921 aagtcggcga gcttcaccgg attcgggtgg aggccggtcg cgaccaggcc cgcgatgtgg
     7981 gccatgtcga ccatgagata ggcgccgacc tcgtcggcga tggcccggaa gcgctcgaag
     8041 tcgagggtgc gggcgtaggc ggaggcgccg gcgacgatca tcctcggctt ggcctcgcgg
     8101 gcgatcttgg cgagctcgtc gtagtcgatc ttctccgtct cgcggttgac gccgtagcgg
     8161 acgaccttgt agagcttgcc ggagaaggag acgggggcgc cgtgggtgag gtggccgccg
     8221 tgggccaggt ccatgcccag gatggtgtcg ccgggcttga gcgcggcgaa atagacgccc
     8281 atgttggcct ggctgcccga atggggctgg acgttggcgt ggtcggcgcc gaagagctcc
     8341 ttggcgcggt cgcgggccag gttctcggcg atgtcgacga actcgcagcc gccgtagtag
     8401 cgctttccgg ggtagccctc ggcgtatttg ttggtgagga ccgagccgac ggcctcctgg
     8461 acggcgcgcg aggtgtagtt ctcgctcgcg atgagctcga ggttctcgcg ctggcggtcg
     8521 gcctccagcg acatggcctt ccagagctcg ggatcctgct tctcgatcag ggacatggtt
     8581 cctcctttgt cctggcaccg aaagggggcg gggccgagag cgattataca gcgcttttcg
     8641 cgaagcgtga acgggaggcg cggccggccc gtcggggcgc ggcctgccgt cagtagaggc
     8701 ccgtg
//
LOCUS       DAOOSJ010000102         8632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5169_length_8632_cov_7.680308, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000102 DAOOSJ010000000
VERSION     DAOOSJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5169_length_8632_cov_7.680308"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLG14_06400"
     CDS             <1..408
                     /locus_tag="PLG14_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48804.1"
                     /translation="ALGDEELLSVHAEIVAKLGRWFEREADKNEIGAFFVMVGKEYCA
                     LGLPVSELTWALLLDRKAVSEFLVENAELEGAHRIYALMAEVDRVSDFFMLGAYYLTK
                     GFLEETFVRLKKGERLTDASVQKYFKDDFFFKE"
     gene            490..834
                     /locus_tag="PLG14_06405"
     CDS             490..834
                     /locus_tag="PLG14_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48805.1"
                     /translation="MTGRLLVVLAVAAASACRYAALLGLSRSIGRRPLLAGLCGGAWA
                     VLLLVLALVLAMAGRREPGLLAWGGAAALVPPLALAAWALARGLVRGLAHGQAHGAAS
                     GRRAGSGREGSA"
     gene            831..1571
                     /locus_tag="PLG14_06410"
     CDS             831..1571
                     /locus_tag="PLG14_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HOX48806.1"
                     /translation="MSAEAGALTISERITEAVSIKTVFTVKAFGLAVPISDTVVATWI
                     VMAVLVLSSFLLTRRLRDVPGKAQSLVEGLVAFANKFAEDNIGHEGRRFVPFIGTVFL
                     FLCVANLLPMLTPVGGFGYEPPFAIKPLTRDINVTSALAVLTMAVVVFSALRSKGLKG
                     FLRSFARPMAFMAPFNALEYAVKPLSLSLRLFGNVLGAFIIMQLAEIVMPVGLPPILG
                     LYFDLFDGLVQAVVFGFLSAVYVAEAIE"
     gene            1581..1805
                     /gene="atpE"
                     /locus_tag="PLG14_06415"
     CDS             1581..1805
                     /gene="atpE"
                     /locus_tag="PLG14_06415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOX48807.1"
                     /translation="MDASSAALVGASIAVLTGIGAGVGIGIATGKTSEAIARQPEASG
                     KISGSFLLGIALAEATAIYGLVVALLLIFR"
     gene            1844..2335
                     /gene="atpF"
                     /locus_tag="PLG14_06420"
     CDS             1844..2335
                     /gene="atpF"
                     /locus_tag="PLG14_06420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOX48808.1"
                     /translation="MLDFSVTFLITIANLAILVLVLKKLLWKPVTDFMDARSRKVREE
                     LEGAAASRARAEELEARYESLLANAEAEGEWVVKDAEDRAREEYRRIVAAAEADAAAI
                     RLRAEERAGLELGRARDELAAEVAALAVAAAARIAGRGLGGADDAAEAEAYIRSAEAG
                     RGR"
     gene            2325..3056
                     /locus_tag="PLG14_06425"
     CDS             2325..3056
                     /locus_tag="PLG14_06425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOX48809.1"
                     /translation="MADSRRAWARALLRAMPEGEARERCAAALEAGASVLGREGAGGT
                     GARAFFLDPSLSRAEKAEALASLFPRAGAAGADPAAGAGLAAGVVTAAGAASLAGAAG
                     GASAAVAAADATTAAGAEPDPGAAAFRRFAALLVERRRSALLPGIAAAYRALLDEEEG
                     LVRIELVTARPLAPGAGEAIGAAFAAVLGKRRFALQASRDPAILGGFVLKAGSVRLDR
                     SAAGRLERLRRELARPLGRGGRQGE"
     gene            3061..4572
                     /gene="atpA"
                     /locus_tag="PLG14_06430"
     CDS             3061..4572
                     /gene="atpA"
                     /locus_tag="PLG14_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOX48810.1"
                     /translation="MERRPDEVTSILLERIAAYADRPEREEVGTVVEAGDGIARVYGL
                     DACVNGELLEFESGAAGMAMNLDEDDVGCVLFSGESLVHEGETVRRTRRVAEIPAGPG
                     LLGRIVDPLGRPLDGLGPVPAGRAMAIERDAPSVIDRKSVGVPLETGVLAVDAMVPIG
                     RGQRELLIGDRQTGKTAIAVDAILNQKGKGVVCVYAAVGQKASTTARVVESLRKLGAL
                     DYSVVVAATAQSSAALQYLCPYSACAAAEFFMEEGKDVLVVYDDLSKHAVAYRALSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLSEAKGGGSMTAIPIVETQGGDISAYIPTNV
                     ISITDGQVFLDSELFYSGVRPAIDVGLSVSRVGGAAQSKAIRKIAGRLRLDLAQYREL
                     AVFAQFGSDLDKATQERLAHGRRLTELLKQGQYAPLPLGEEIALLYAAVNGYFSAFKP
                     EELGEARATLLRLLSYRHAELVARLGREGELSEESEGALKAALEAFKGAKKAE"
     gene            4584..5444
                     /gene="atpG"
                     /locus_tag="PLG14_06435"
     CDS             4584..5444
                     /gene="atpG"
                     /locus_tag="PLG14_06435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOX48811.1"
                     /translation="MRDVRLRMRSVRQSLQVTQAMKLISTSKLRKARRRLEETRPYFE
                     AIREAMHDIVELGAVGSGAWFDAREGKAARKTAVLVVSSDRGLAGGYNAQVIRFVEER
                     CPPGSILLPIGSIGKRHFMDKDYVLLEDFPTSPKEPTVYEARDIAAFVAELFLAGRID
                     EFKVVYTRLESVVRQSPEILDLLPLAHERLEPGRRARRQAPRFSCLPDEETVFDSLVP
                     KYLKGVVYGALVESFASEQAARMTAMDAASKNAKQMLDRLQLLYNRARQAAITGEVSE
                     IVAGAAALDS"
     gene            5488..6879
                     /gene="atpD"
                     /locus_tag="PLG14_06440"
     CDS             5488..6879
                     /gene="atpD"
                     /locus_tag="PLG14_06440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOX48812.1"
                     /translation="MKGNMGKVARVTGPILDLRFEAGEAPRILDAVEIERPDGSTLLA
                     EVLLHKGDGIVRCVAMEATEGLARGLPARDTGSPITVPVGREVLGRMFDVAGRPIDGG
                     PAVQAARSPIHREAPPLEDQMGGAELFETGIKVIDLVAPYAKGGKIGLFGGAGVGKTV
                     VIMELIRNIASVHQGYSVFAGVGERSREGNDLWREMNESGVVKNTALVFGQMNEPPGA
                     RMRVALSGLAMAEHFRDELGQDVLLFVDNIFRFVQAGSEVSALLGRIPSAVGYQPTLA
                     NEIGALEERITSTKRGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSRRIVEKGI
                     YPAVDPLASTSRVLDPLVVGEEHYRVARRVQEVLQRYRELQDIIAILGMDELGPEDRL
                     AVHRARKIERFFSQPFFVAEQYTGIPGRYVKLADTIRGFKEIVEGQCDHIPEQAFYMA
                     GTIDEVRERAEKG"
     gene            6884..7306
                     /gene="atpC"
                     /locus_tag="PLG14_06445"
     CDS             6884..7306
                     /gene="atpC"
                     /locus_tag="PLG14_06445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOX48813.1"
                     /translation="MSPFRCAILTPYRLLFSASVESVTFATTDGEMEILAGHEPVVAP
                     VRIGEVRLRGAEGLKVAAVSEGFASVRPDRVELFVDAAEWPEEIDRGRAAKSLERAEE
                     RLKEVTLPWELERAKSSAARARARLRAAGRLAGDAQRP"
     assembly_gap    7320..7419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7543..7737)
                     /locus_tag="PLG14_06450"
     CDS             complement(7543..7737)
                     /locus_tag="PLG14_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48814.1"
                     /translation="MKQFAIKSAGTAPSLLELLGETEGGFYVRIVHRHEDWEDVKEDF
                     MTRDLFETCVRTGYIAELSA"
     gene            complement(7783..8304)
                     /locus_tag="PLG14_06455"
     CDS             complement(7783..8304)
                     /locus_tag="PLG14_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOX48815.1"
                     /translation="MIHGFEGKRPRLHESAFVAWNAEVSGDAELAEGSSVWYGAVIRA
                     DIGPVRLGRLSNLQDGAVIHVDEGSPCVLGEGVTVGHRAVLHSCSVGDYALVGMGAIV
                     LDGAEIGSESIVGAGALVTQGKKFPPRSMILGSPAKVVRELKAEEVESLHHHAEEYAR
                     LAKRTREGSEELR"
     gene            complement(8432..>8632)
                     /locus_tag="PLG14_06460"
     CDS             complement(8432..>8632)
                     /locus_tag="PLG14_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48816.1"
                     /translation="GRYGMAKAAKKGEWNVQAAAVQDAIVKAGDPAKIALKPDTTSDA
                     VSGASIHLSGVVLAVEALKAAR"
BASE COUNT         1183 a         3078 c         3009 g         1262 t
ORIGIN      
        1 gccctgggcg acgaggagct cctctccgtc cacgcggaga tcgtggccaa gctggggcgc
       61 tggttcgagc gcgaggcgga caagaacgag atcggggcct tcttcgtgat ggtgggcaag
      121 gagtactgcg ccctgggcct gccagtctcc gagctcacct gggcccttct cctggaccgc
      181 aaggcggtca gcgagttcct cgtggagaac gccgagctcg agggcgcgca ccgcatctac
      241 gccctgatgg cggaggtcga cagggtctcg gacttcttca tgctcggcgc ctactacctc
      301 accaagggct tcctggagga gaccttcgtg cggctcaaga agggcgagag gctgaccgac
      361 gcgtccgtcc agaagtactt caaggacgat tttttcttca aggagtaggc gccgaggcgc
      421 gcctcaaggg gtatcgatgc ggtttcgtat gacgcgcaaa cgcgccggcc tcccgaagga
      481 ggccgggccg tgacgggccg cctcctcgtc gtcctggccg tggccgcggc gagcgcctgc
      541 cgctacgccg ccctgctcgg cctctcccgc tccatcgggc ggaggcccct cctcgcgggc
      601 ctctgcggcg gagcctgggc cgtccttctc ctcgtcctcg ccctcgtgct cgccatggcg
      661 gggcggcggg agcccggcct cctcgcctgg ggcggcgcgg cggcccttgt cccgccccta
      721 gccctcgccg cctgggccct cgcccgcggc ctggttcgag gcctcgccca cggccaggcc
      781 cacggcgccg cctcgggtcg gcgggccggg agcggaaggg aaggaagcgc atgagcgccg
      841 aggccggcgc gctcacgatc agcgagcgca tcaccgaggc cgtctccatc aagacggtct
      901 tcacggtcaa ggccttcggc ctcgccgtgc ctatctccga cacggtggtc gcgacctgga
      961 tcgtgatggc cgtcctcgtc ctttcctcct tcctcctcac gcggaggctc agggacgtgc
     1021 cggggaaggc ccagtccctc gtggagggac tcgtggcctt cgcgaacaaa ttcgctgagg
     1081 acaacatcgg ccacgagggc cggcgcttcg tccccttcat cgggacggtc ttcctcttcc
     1141 tctgcgtcgc caacctcctt cccatgctca cgcccgtcgg aggcttcggc tacgagccgc
     1201 ccttcgccat caagccgctc acgagggaca tcaacgtgac ctccgccctc gccgtcctca
     1261 cgatggccgt cgtcgtcttc tccgccctga ggagcaaggg cctcaagggc ttcctccgga
     1321 gcttcgcgcg gcccatggcc ttcatggcgc ccttcaacgc gctcgagtac gccgtgaagc
     1381 cgctctcgct ctcgctgcgg ctcttcggca acgtcctcgg cgccttcatc atcatgcagc
     1441 tcgcggagat cgtgatgccc gtcggcctcc cgccgatcct gggcctctac ttcgacctct
     1501 tcgacggcct cgtgcaggcc gtggtcttcg gcttcctgtc ggcggtgtat gtcgccgagg
     1561 cgatagaata gggggaatca atggacgcat catcggcggc cctcgtcggg gcgagcatcg
     1621 cggtcctgac cggcatcgga gccggcgtgg gcatcggcat agcgacgggc aagacctcgg
     1681 aggccatcgc gaggcagccc gaggcgagcg gcaagatctc ggggagcttc ctcctcggca
     1741 tcgccctcgc ggaggcgacc gcgatctacg gactcgtcgt cgccctcctc ctcatcttcc
     1801 ggtgaggcgg ggagcctagg gccagaggcg ggagggtagg aacttgctcg acttctccgt
     1861 caccttcctc atcacgatcg cgaacctcgc gatcctcgtc ctcgtcctca agaagctcct
     1921 ctggaagccc gtgaccgact tcatggacgc ccggagccgc aaggtgcgcg aggagctcga
     1981 gggagcggcc gcgtccaggg ctcgggcgga ggagctcgag gcgcgctacg agtccctcct
     2041 cgccaacgcc gaggccgagg gcgagtgggt cgtgaaggac gccgaggacc gggcccggga
     2101 ggagtaccgc cgcatcgtcg ccgcggccga ggccgacgcg gccgccatcc gcctgcgcgc
     2161 ggaggagcgg gccggcctgg agctcggccg cgcgcgggac gagctcgccg cggaggtggc
     2221 cgccctcgcg gtggccgccg ccgcgcggat cgcgggccgt ggcctgggcg gcgcggacga
     2281 cgcggccgag gccgaggcct acatacggag cgcggaggcc ggccgtggcc gatagccgcc
     2341 gggcctgggc ccgggccctc ctccgcgcca tgcccgaggg cgaagcacgc gagcgctgcg
     2401 ccgccgcgct ggaggccggg gcctcggtcc tgggccgcga aggcgcgggc gggacaggag
     2461 cgagggcctt cttcctggat ccctcgcttt cccgcgcgga gaaggcggag gccctggctt
     2521 cgcttttccc ccgggctggc gccgcgggcg cggacccagc cgcgggcgcg ggcctagccg
     2581 ccggcgttgt taccgcggcc ggcgcggctt cccttgctgg cgccgcgggc ggggcctccg
     2641 ccgccgtcgc cgcggccgac gctaccaccg cggccggcgc ggagcccgac cccggggcgg
     2701 cggccttccg gcgtttcgcg gccctcctcg tcgagagaag gcgttccgcc ctccttcccg
     2761 ggatagcggc cgcctatcgc gccctcctgg acgaggagga ggggcttgtc cgcatcgagc
     2821 tcgtcaccgc gcgacccctc gcgccgggcg ccggggaggc gataggcgcg gccttcgcgg
     2881 ccgttctcgg aaagcggcgc ttcgccctcc aggcctcgcg cgacccggcc atcctcgggg
     2941 gcttcgtcct caaggcggga tcggtccgcc tggaccgttc cgcggcgggg cgcctggagc
     3001 gcctgcgccg cgagctcgcc cggcccctgg gccggggcgg gcgacaagga gaatagcgtc
     3061 atggagcggc gtcccgacga agtcacgagc atactcctcg agcggatagc ggcctacgcg
     3121 gaccgcccgg agcgcgagga ggtcggcacg gtcgtggagg cgggagacgg catagcgcgc
     3181 gtctacggcc tcgacgcctg cgtcaacggc gagctcctcg agttcgagtc gggggcggcc
     3241 ggcatggcga tgaacctgga cgaggacgac gtcggctgcg tcctcttctc gggcgagagc
     3301 ctcgtccacg agggggagac cgtgaggcgc acgaggcgcg tcgcggagat cccggccggc
     3361 cccggcctcc tcgggaggat cgtcgatccc ctggggcggc ccctcgacgg cctgggtccc
     3421 gtcccggccg ggcgcgccat ggccatcgag cgcgacgcgc cctcggtgat cgaccgcaag
     3481 agcgtcggcg tccccctgga gaccggcgtc ctcgccgtgg acgcgatggt tcccatcggt
     3541 cgcggccagc gcgagctcct catcggcgac aggcagaccg gcaagaccgc tatcgccgtc
     3601 gacgcgatcc tgaaccagaa gggcaaggga gtcgtctgcg tctacgcggc cgtgggccag
     3661 aaggcgagca cgacggcccg cgtcgtggag tcgctccgga agctcggcgc cctcgactac
     3721 tccgtcgtcg tcgcggccac cgcccagagc tcggccgccc tgcagtacct ctgcccctac
     3781 tcggcctgcg ccgccgcgga gttcttcatg gaggagggca aggacgtcct tgtcgtctac
     3841 gacgacctct ccaagcacgc ggtcgcctat cgcgccctct ccctgctcct ccgcaggccg
     3901 ccggggcgcg aggcctatcc gggcgacgtc ttctacctcc actcgcgcct cctcgagcgc
     3961 gcggcgcggc tctccgaggc caagggcggc ggctccatga ccgcgatccc gatcgtggag
     4021 acccagggcg gcgacatctc cgcctacatc ccgaccaacg tcatctccat cactgacggc
     4081 caggtcttcc tcgactccga gctcttctac tcgggggtgc ggcccgccat cgacgtcggg
     4141 ctctccgtct cccgcgtcgg cggcgcggcc cagtccaagg cgatcaggaa gatcgcggga
     4201 cgcctccgcc tggacctcgc gcagtaccgg gagctcgccg tcttcgccca gttcggctcc
     4261 gacctggaca aggcgaccca ggagcggctc gcgcacggac ggaggctcac cgagctcctc
     4321 aagcagggcc agtacgcgcc ccttcccctc ggggaggaga tcgcgctgct ctacgcggcg
     4381 gtcaacggct acttctccgc cttcaagccc gaggagctcg gcgaggcgcg cgcgaccctc
     4441 ctgcgcctcc tctcctacag gcacgcggag ctcgtggcga ggctcggccg ggagggcgag
     4501 ctgtccgagg agtcggaagg cgcgctcaag gccgccctcg aggccttcaa gggcgcgaag
     4561 aaggccgagt agcgatggag acgatgcggg acgtccggct gcgcatgcgg agcgtgcgcc
     4621 agtcgctcca ggtgacccag gcgatgaagc tcatctcgac ctccaagctc aggaaggcgc
     4681 ggaggcgcct cgaggagacg cggccctact tcgaggccat cagggaggcg atgcacgaca
     4741 tcgtcgagct cggggcggtc ggctccgggg cctggttcga cgcgcgggag gggaaggcgg
     4801 ccaggaagac ggccgtcctc gtcgtgagct cggaccgggg cctggccggc ggctacaacg
     4861 cccaggtcat ccgcttcgtg gaggagcgct gcccgccggg ctccatcctc ctgccgatcg
     4921 gctcgatcgg caagcggcac ttcatggaca aggactacgt cctcctggag gacttcccga
     4981 cctcgcccaa ggagccgacg gtgtacgagg cgagggacat cgcggccttc gtggccgagc
     5041 tcttcctcgc gggacggatc gacgagttca aggtcgtcta cacgaggctc gagtcggtgg
     5101 tgcggcagtc ccccgagatc ctcgacctcc tcccccttgc ccacgagcgg ctcgagccgg
     5161 gccgcagggc gcggcggcag gccccgcgct tctcctgcct ccccgacgag gagacggtct
     5221 tcgactccct cgtgcccaag tacctgaagg gggtcgtcta cggcgccctc gtcgagtcct
     5281 tcgcgagcga gcaggcggcg cgcatgacgg cgatggacgc ggcctccaag aacgccaagc
     5341 agatgctgga ccggctccag ctcctctaca acagggcccg ccaggccgcc atcaccggcg
     5401 aggtctccga gatcgtcgcg ggcgcggcgg ccctggactc gtgagcggcc ggcccgcggg
     5461 agcggggcgg cgtggaaagg aagtcgcatg aaagggaaca tgggcaaggt agccagggtc
     5521 acgggcccca tcctcgacct ccgcttcgag gcgggcgagg cgccgcggat cctcgacgcc
     5581 gtcgagatag agcggcccga cggctccacc ctcctcgcgg aggtccttct ccacaagggc
     5641 gacggaatcg tccgctgcgt cgcgatggag gcgacggagg gcctggcgcg aggcctgccc
     5701 gcccgcgaca cgggctcgcc gatcacggtc cccgtcggcc gggaggtcct gggccggatg
     5761 ttcgacgtcg cggggcggcc catagacggc ggccccgcgg tccaggcggc gcgctcgccc
     5821 atccaccgcg aggccccgcc gctcgaggac cagatgggcg gcgccgagct cttcgagacc
     5881 ggcatcaagg tcatcgacct cgtcgcgccc tacgccaagg gcggcaagat cggcctcttc
     5941 ggcggcgcgg gcgtgggcaa gaccgtcgtc atcatggagc tcatcaggaa catcgcgagc
     6001 gtgcaccagg gctactcggt cttcgccggc gtgggcgagc ggagccgcga gggcaacgac
     6061 ctctggcggg agatgaacga gtcgggcgtc gtcaagaaca ccgccctggt cttcggccag
     6121 atgaacgagc cgcccggggc gcgcatgcgc gtcgccctct ccggcctcgc gatggccgag
     6181 cacttcaggg acgagctcgg acaggacgtc ctcctcttcg tggacaacat cttccgcttc
     6241 gtccaggccg gcagcgaggt gtcggccctc ctcgggcgca tcccgagcgc ggtgggctac
     6301 cagccgacgc tggccaacga gatcggcgcc cttgaggagc gcataacctc cacgaagcgg
     6361 ggctcgatca cctccgtcca ggccgtctac gtgcccgcgg acgacctcac ggacccagcc
     6421 cccgcgacca ccttcaccca cctggacgcc accaccgtcc tctcccggag gatcgtcgag
     6481 aagggcatct atcccgctgt cgatcccctc gcctccacct cccgggtcct cgaccccctg
     6541 gtcgtcgggg aggagcacta ccgggtggcg cgccgcgtcc aggaggtcct gcagcgctac
     6601 cgggagctcc aggacatcat cgccatcctg ggcatggacg agctcgggcc ggaggacagg
     6661 ctggccgtgc accgggcgcg gaagatcgag cgcttcttct cccagccttt cttcgtcgcg
     6721 gagcagtaca cgggcattcc gggccgctac gtgaagctcg cggacacgat ccgcggcttc
     6781 aaggagatcg tcgagggcca gtgcgaccac atcccggagc aggccttcta catggcgggg
     6841 acgatcgacg aggtccgcga gcgggccgag aagggctagg gccgtgtctc cctttcgctg
     6901 cgccatcctc acgccctaca ggctcctctt ctccgcctcc gtcgagtccg tgaccttcgc
     6961 caccacggac ggcgagatgg agatcctcgc gggccacgag cccgtcgtgg cgcccgtcag
     7021 gatcggcgag gtccgcctcc gcggcgcgga ggggctcaag gtcgccgccg tctccgaggg
     7081 cttcgcctcc gtcaggcccg accgggtcga gctcttcgtc gacgcggcgg aatggccgga
     7141 ggagatagac cgcgggcgcg ccgccaagtc gctcgagcgg gccgaggagc ggctcaagga
     7201 agtgacgctt ccctgggagc tcgagcgcgc gaagagttcg gccgcccgag cccgcgcccg
     7261 cctccgcgcg gccggccgcc tcgcggggga cgcccagagg ccctagcccg gcgccgcgcn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcgcgccaa cgcgccgtcc
     7441 ggcggcgccg ccgtgcctcg cttcccgccg cgccatgaaa gcgagaggcc gccccggtcg
     7501 ggcggcctcg tttttagctg gctttgagga gaggagaggt ctctaggccg agagctcggc
     7561 gatgtagccc gtgcggacgc aggtctcgaa gaggtccctc gtcatgaagt cctccttgac
     7621 gtcctcccag tcctcgtggc ggtgcacgat gcggacgtag aagccgccct cggtctcgcc
     7681 gaggagctcg aggagggaag gggccgtacc ggcgctcttg atcgcgaatt gcttcatgcg
     7741 cgtactcctt aggctaagta tcggagcggc gcgcgtccgg gtttagcgga gctcttcgga
     7801 gccttccctc gtcctcttgg cgagcctcgc gtactcctcc gcgtgatgat ggaggctctc
     7861 gacctcctcg gccttgagct cgcggacgac cttggccggg cttccgagga tcatcgagcg
     7921 cggggggaac ttcttgccct gggtcacgag ggcgccggcg ccgacgatgg actcgctccc
     7981 gatctcggcg ccgtcgagca cgatggcgcc catcccgacg agggcgtagt cgccgaccga
     8041 gcaggagtgg aggacggcgc gatgcccgac ggtgacgccc tccccgagga cgcaggggct
     8101 tccttcgtcc acgtggatga cggccccgtc ctgcaggttc gaaaggcggc ccaggcgcac
     8161 ggggccgatg tcggcgcgga tgacggcgcc ataccagacg gaggagccct cggcgagctc
     8221 tgcgtcgccc gacacctcgg cgttccaggc cacgaaggcg ctctcgtgca ggcgggggcg
     8281 cttgccctcg aatccgtgga tcatcgtttc ctcctcggaa aagaaaagcg gcccggctta
     8341 gagcgggccg cccggctccc ccggcatggc cggaggagcc ccgattcgcc tctgggcttt
     8401 cgcctagcgg gctcgcccta gcgcgctcgc cctagcgggc ggccttgagg gcctcgacgg
     8461 cgagcacgac gccggagagg tggatggagg cgccggagac ggcgtcggag gtggtgtcgg
     8521 gcttgagggc gatcttggcg gggtcgccgg ccttgacgat ggcgtcctgg accgcggcgg
     8581 cctggacgtt ccactcgccc ttcttggcgg ccttggccat gccgtagcgg cc
//
LOCUS       DAOOSJ010000103         8593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5192_length_8593_cov_5.427032, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000103 DAOOSJ010000000
VERSION     DAOOSJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5192_length_8593_cov_5.427032"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PLG14_06465"
     CDS             complement(<1..1857)
                     /locus_tag="PLG14_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX48817.1"
                     /translation="MESVTFESLGLGPDTLAAIARKGFEEPTPIQALTIPRLLADGPD
                     LIARARTGTGKTAAFGLPLVERLSTPTGKVGALILVPTRELALQVSGEILSLRTTDRP
                     RVAPVYGGASMGEQLRRLAAGVDIVVGTPGRVMDHLERGTLDLSALRFLVLDEADEML
                     DMGFIEDIETIMEKCGPDRRVVLFSATMPAGIIHVAKRRLGKYEIVEDETEAVATALA
                     EQVWLEVREGDKLEALCRIIDSEEDFFGIVFTATRIEADRISKALEERGYESEALHGD
                     ITQDRRERILARFRERRVRILVATDVAARGIDVERISHVVNWTLPHDPEAYVHRVGRT
                     GRAGNAGTAITFVTPDEYRKLFRFKHAAGTGFKKGSVPAVSEVIGARRDRIKAKILAR
                     AVSAALVPQAEEASAEASAEAAAEAAAGNQATAAAEPQAAETAAPEPQAEAGAEAQAV
                     ASEAAGVSASEAAAETAAEAAAPEAATASPEALAKAKAEKAAARKAARAAKKAASPEA
                     AELWLGLADELLARLPPREALAAALFESFGAELDPARYREIADVSVDAAATARLFVGA
                     GRRDGATPRALAAMVKRLTGLPDRLVGGIEIYDEFSFLSVPFEAAERVIAAAR"
     gene            2217..3236
                     /locus_tag="PLG14_06470"
     CDS             2217..3236
                     /locus_tag="PLG14_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOX48818.1"
                     /translation="MRSFASDNNSGASPEIVRAVAAANEGHAVGYGDDPWTERARSLF
                     KREFGPEAEAFFVLNGTGANVLALSLAAGPSTCVLCAEGAHIDVDETGAPEAAGIKVV
                     PLAARLGKLEPEALREALGALGNVHAAQPSCLSISQPTERGAVYSKPEIEELCRIAHS
                     AGLLVHVDGARLANAAVALGLPFRAFTVDAGVDLLSFGGMKNGLLFGEVLLVFRHELA
                     RRTPYLRKARLQLASKMRFVSAQYEAYLEGGLWKRNAEAANASARSLAGLVRGIEGVE
                     LEGEVETNALFARLSEDAAERLRQEYFFYDWEGGLVRWMTSFDTTEADIEGFAAALRR
                     ALSGT"
     assembly_gap    3443..3542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3543..4726)
                     /locus_tag="PLG14_06475"
     CDS             complement(<3543..4726)
                     /locus_tag="PLG14_06475"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOX48819.1"
                     /translation="MRTSPRTAALTHPKTRSHSRLLAALGSALFCLAALFVLLSLSFK
                     GAMLEARKAELGRLTALASYALEPVLVRERAGLLSRDEARAEAAALLRTFVYGDDTRK
                     NYLFMSAYDGTMLVQPFEPWLEGSSQWDLRDADGVFPVRELVRAARSGGGYVAYRHLA
                     PGSRRPETKVSFVLPVEGLEAYLGTGFYRSDIDHQAFRLAAPVLAGAGLIVLAFALFV
                     AYGLRPFYAAYRRLLAAFAAAAESPGKPPVVTLQGFRPGSEARCLLESFNGLAERLAA
                     YDEARGRLELRLKGENAEKHLLLKEVHHRVKNNLQIVSSLLSLQARARKEARCSPCLG
                     DSTARIQSMAEVHELLYESGDFSRVDFARYAERLVVSAAEAAGMGARVDLRLEPLSLS
                     LD"
     gene            4981..7008
                     /locus_tag="PLG14_06480"
     CDS             4981..7008
                     /locus_tag="PLG14_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48820.1"
                     /translation="MKRRFSITAKLILFISALFIGVIGAVATVIFFTFRDSLDAQLKE
                     QSATTSGYIREEIRSWLLPKDAAVSTHVMAAQMAWPRLEQLQAMYKAGVAADKDLTDI
                     YIFESKSWKEGGRVIIASGWIPPADYDQYKRTWFTNSLATAEVIHSAPYVDSITGKLV
                     VTVSQRAMVDGKPIGVVGADMFITRVGEIVGAKKLSANGKTYLVDKDGLLITHEDPEK
                     ILKANLFEGNILSPLKDKILKGGETTFGILRKEGAYYASIRIPELNAILVTAGPVSDI
                     YGALNAFLARLALIVGLGIAVAVLVLFAIARSFTRPILALTAVSKRLSEGRLDFDAAK
                     EGLAGRNDELGELSEAFGLMVLRVAEVVDEVKSSVEVLLRGSSNLNQASVAMSQGATE
                     QAASTEEVSASLEQMGGNIRNNADNAKETERISRKAAKDTEEGSKAVLETVGAMKDIS
                     QKILIIEEIARQTNLLALNAAIEAARAGEAGKGFAVVASEVRKLAERSQKAATEIGEL
                     SVKSVGVSEKAGSMLDQIVPDIQKTSQLVQEISAASNEQSQGTDQITKAIMQLDKVVQ
                     ENAASSEEIAGMTEEIARLAQNLSARISFFKTKGGRAEGAAQAAAQDSGRGRPKAQSA
                     GQTSAPRGKPAEGRSDPKPDPRPAPKAQIRPSRETGITIKSGAEDEDFEEF"
     gene            complement(7136..7891)
                     /gene="tatC"
                     /locus_tag="PLG14_06485"
     CDS             complement(7136..7891)
                     /gene="tatC"
                     /locus_tag="PLG14_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOX48821.1"
                     /translation="MTFTDHLAELRKRLVVSCAAFLAAGFGCFSFAEKLAEFVMRPAG
                     TLQFVYLSPPELFVSYVKLALVAGLALSLPVILFELWLFVSPALSRRERGSVFLCLFF
                     GAFFFVAGAAFAYYVILPFTIKFFLSYSNPRVEAVFAIKDYFDFVSGLVLGFGAAFEL
                     PMAAALLGAFGLLKAELLVRGRSVAVLGIFIAAAILTPPDVVSQVLLALPMLGLYELS
                     ILILKVQGRGRARREALAEASARADGEGSPEPV"
     gene            complement(8049..8315)
                     /locus_tag="PLG14_06490"
     CDS             complement(8049..8315)
                     /locus_tag="PLG14_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOX48822.1"
                     /translation="MFGRIGPAELILVLVIALVIFGPKKLPEIGKAVGQAIREFKKHS
                     DKVTEDIAKATDADAGSEAQAPRIEAVAKPAEAPEAAKSKAKKA"
     gene            complement(8319..>8593)
                     /locus_tag="PLG14_06495"
     CDS             complement(8319..>8593)
                     /locus_tag="PLG14_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOX48823.1"
                     /translation="HNAVDKVLGRGLLDGADFSRSVLLTSGRIAADMAQKAAKAGLPV
                     LVSRSIPTTEAYAFAREAGITLIGRIGTPSPIVYTRPELVRPEAKE"
BASE COUNT         1357 a         2801 c         3116 g         1219 t
ORIGIN      
        1 gcgcgccgcg gcgatgaccc gctcggcggc ctcgaagggc acggagagga aggagaactc
       61 gtcgtagatc tcgatgccgc cgaccaggcg gtcggggagg cccgtgaggc gcttcaccat
      121 cgcggccagg gcccggggag tcgcgccgtc gcgcctgccc gcgccgacga agagcctcgc
      181 ggtcgcggcc gcgtcgacgg agacgtcggc tatctcgcgg tagcgcgccg ggtcgagctc
      241 ggcgccgaag gactcgaaga gggcggcggc cagggcctcg cggggcggaa ggcgggcgag
      301 gagctcgtcc gcgaggccga gccagagctc ggcggcctcg ggggaggcgg ccttcttggc
      361 cgcgcgggcg gccttgcggg cagcggcctt ctcggccttc gccttggcga gggcttcggg
      421 gctcgccgtg gcggcctcgg gcgcggcggc ttcggcggcg gtctcggcgg cggcctcgga
      481 ggcggacacg ccggcggcct cggaggcgac ggcctgagcc tcagctccgg cctcggcctg
      541 cggctccggg gccgcggtct ccgcggcctg aggctcggcg gcggccgtgg cctgattccc
      601 ggcggccgcc tcggccgcgg cttccgccga ggcctcggcc gaggcctcct cggcctgggg
      661 cacgagggcg gcggagacgg cgcgggccag gatcttcgcc ttgatgcggt cgcgcctggc
      721 gccgatgacc tcggagacgg cggggaccga gcccttcttg aagccggtgc ccgcggcgtg
      781 cttgaagcgg aagagcttcc ggtactcgtc gggggtcacg aaggtgatcg cggtgccggc
      841 gttgccggcc cggcccgtgc ggccgacgcg gtgcacgtag gcctcggggt cgtggggcag
      901 ggtccagttc acgacgtgcg atatgcgctc gacgtcgatg ccgcgggccg cgacgtcggt
      961 cgcgaccagg atgcggacgc ggcgctcgcg gaagcgggcc aggatgcgct cgcgcctgtc
     1021 ctgcgtgatg tcgccgtgga gggcctccga ctcgtagccg cgctcctcca gggccttgga
     1081 gatgcggtcc gcctcgatcc tcgtcgccgt gaagacgatg ccgaagaagt cctcctcgga
     1141 gtcgatgatg cggcagaggg cctcgagctt gtcgccttcg cggacctcga gccagacctg
     1201 ctccgcgagc gcggtggcca cggcctcggt ctcgtcctcg acgatctcgt acttgcccag
     1261 gcggcgcttc gcgacgtgga tgatgccggc gggcatcgtc gcggagaaga ggacgacgcg
     1321 gcggtcgggg ccgcacttct ccatgatggt ctcgatgtcc tcgatgaagc ccatgtcgag
     1381 catctcgtcg gcctcgtcca ggacgaggaa gcgcagggcg gagaggtcga gggtgccgcg
     1441 ctccaggtgg tccatgacgc ggccgggggt gccgacgacg atgtcgacgc cggccgcgag
     1501 gcgcctgagc tgctcgccca tggaggcgcc gccgtagacg ggggcgacgc ggggcctgtc
     1561 ggtcgtgcgc agggagagga tctctcccga gacctggagg gcgagctcgc gggtcgggac
     1621 caggatgagg gcgccgacct tgcccgtcgg cgtggagagg cgctccacga gggggaggcc
     1681 gaaggcggcg gtcttgccgg tgccggtcct ggcgcgggcg atgaggtcgg ggccgtccgc
     1741 gaggaggcgg gggatggtga gggcctggat gggggtgggc tcctcgaagc ccttccgcgc
     1801 gatggcggcc agggtgtcgg ggccgaggcc gaggctttcg aaggtgacgc tttccatgta
     1861 cttccttggg gttccgcgct ctcgcggagc cgtgtgggcc gctgtcgggg ccgatccgag
     1921 ctcgcgccgc ccctgcccgg ccgcccttcc cgaggagtct cgagaaggaa gcccaaggag
     1981 cgcgctcgtg cgtatggtgg gagccccgcg ggggaaagcc gcgttgtata ctgcctgtat
     2041 tcggttcgct tcgaagatgg gccttggttc gcggaggctt cgctaggcct tgattctacc
     2101 ccctttgggc cctgcgttgc taggccccga tgcgaagaaa gcccagcgca ttgcatccaa
     2161 ccgcaaagga tgctattttc gcagggaacc gggcggaaga acgaggaggg atgaccatga
     2221 ggagcttcgc gagcgacaac aattccggcg cttccccgga gatcgtccgg gccgtcgcgg
     2281 cggccaacga gggccacgcc gtcggctacg gcgacgatcc ctggaccgag cgggcccgct
     2341 ccctcttcaa gcgcgaattc ggcccggagg ccgaggcctt cttcgtcctc aacggcacgg
     2401 gcgccaacgt cctggccctc tctctcgccg ccggcccctc gacctgcgtc ctctgcgcgg
     2461 agggcgccca catcgacgtc gacgagacgg gagcgcccga ggcggccggc atcaaggtcg
     2521 tccccctggc cgcgaggctg ggcaagctcg agccggaggc cctgcgcgag gccctgggcg
     2581 ccctggggaa cgtccacgcg gcccagccct cctgcctctc catctcccag ccgacggagc
     2641 gcggcgccgt ctactcgaag cccgagatcg aggagctctg ccgcatcgcc cactccgccg
     2701 gcctcctggt ccacgtggac ggcgcgaggc tggccaacgc ggccgtcgcc ctaggcctcc
     2761 ccttccgcgc cttcaccgtc gacgcggggg tagacctcct ctccttcggc ggcatgaaga
     2821 acggcctcct cttcggcgag gtcctcctgg tcttccggca cgagctcgcg aggcggacgc
     2881 cctacttgag gaaggcgcgg ctccagctcg cctcgaagat gcgcttcgtc tccgcccagt
     2941 acgaggccta cctggagggc ggcctgtgga agaggaacgc ggaggcggcc aacgcgtcgg
     3001 cccgctccct ggctggcctg gtccgcggga tcgagggcgt cgagctcgag ggcgaggtcg
     3061 agacgaacgc cctcttcgcg cgcctgagcg aggacgcggc cgagcggctc aggcaagagt
     3121 acttcttcta cgactgggaa ggggggctcg tccgctggat gacgagcttc gacacgacgg
     3181 aggccgacat cgaaggcttc gcggccgcgc tgaggcgggc cctctcgggg acctgaggcc
     3241 aggcgggccc tggctggggc ccgaggcagg ggcaggcgcc ggagagacgc cgccgcgctc
     3301 cggttggccg cgcccgggcc gcccccggct aggcggaggg caggggaaat tccaggacga
     3361 ggcgagcgcc gttcgagctc gaggcgctga tctccccctt gagctggccg gccaaggtct
     3421 ccatgagcat gaggccgagg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nntggtcgag ggagagggag aggggctcga ggcggaggtc gacgcgggct cccatgcccg
     3601 cggcctccgc ggcggagacg acgagccttt cggcgtagcg ggcgaagtcc acccgggaaa
     3661 agtcgccgct ctcgtagagg agctcgtgga cctcggccat gctctggatc ctcgcggtgg
     3721 agtcgcccag gcagggagag cagcgcgcct ccttcctcgc cctggcctgg aggctcagga
     3781 gggaggagac gatctggaga ttgttcttga cccggtggtg cacctccttg aggaggaggt
     3841 gcttctccgc gttctcgccc ttgagcctga gctccaagcg gccgcgggcc tcgtcgtagg
     3901 cggccaggcg ctccgcgagg ccgttgaagg actcgaggag gcagcgggcc tccgagcccg
     3961 gcctgaaccc ctggagggtg acgacgggag gcttgcccgg gctctccgcc gcggcggcga
     4021 aggccgcgag gaggcggcgg taggccgcgt agaaggggcg gaggccgtag gcgacgaaga
     4081 gggcgaaggc gaggacgatg aggccggccc ccgcgaggac tggcgcggcg agcctgaagg
     4141 cctggtggtc gatgtcgctc cggtagaagc ccgtgcccag gtaggcctcg agcccctcca
     4201 cggggaggac gaaggagacc ttcgtctccg gccgcctgga cccgggagcc aggtggcggt
     4261 aggcgacgta gccgccgccc gagcgggccg cgcggacgag ctccctgacg ggaaagacgc
     4321 cgtcggcgtc gcgtaggtcc cactgggagc ttccctcgag ccagggctcg aagggctgga
     4381 cgagcatcgt gccgtcatag gcgctcatga agaggtagtt cttccgggtg tcgtcgccgt
     4441 agacgaaggt gcggaggagg gcggcggcct ccgcgcgggc ctcgtcgcgc gagaggaggc
     4501 ccgcccgctc ccggaccagg acgggctcga gggcgtagga ggccagggcg gtgagccgcc
     4561 cgagctcggc cttcctggcc tcgagcatgg cgcccttgaa ggacagggag aggagcacga
     4621 acagggccgc caggcagaag agggccgagc cgagggctgc gaggagcctg gaatgggatc
     4681 gggtcttggg atgggttaaa gcggccgtac ggggcgaggt tcgcaagtcg agctcctttt
     4741 ttatatggcg taaggcaacc caactttatt tattatctcc ataataatca tccggccaga
     4801 tttgtcaagt attaaattct ctatgttaac caaagacgat atgccttttt tgactttgcc
     4861 agggaactag gctaggatag gcagtgcaag acaaggaacg gggcccgccc tcatgcccgt
     4921 ctaggaatct aaaaaagatg ggcgaagccg ttccgacgac caacgcgcaa ggagcggacc
     4981 atgaagcgca gattctccat caccgcgaag ctcatcctct tcatctcggc gcttttcata
     5041 ggcgtcatag gggcggtcgc gaccgtcatc ttcttcacct tcagggactc gctggacgcc
     5101 cagctcaagg agcagtcggc gaccacgagc ggctacatcc gcgaggagat ccgctcctgg
     5161 ctcctgccca aggacgcggc cgtctccacc cacgtcatgg cggcccagat ggcctggccc
     5221 cggctcgagc agctccaggc catgtacaag gccggcgtcg ctgcggacaa ggacctcacg
     5281 gacatctaca tcttcgagtc gaagtcctgg aaggaggggg gccgggtcat catcgcctcg
     5341 ggctggatcc cgcccgctga ctacgaccag tacaagcgga cctggttcac gaactccctg
     5401 gcgacggcag aggtcatcca ctcggcgccc tacgtcgaca gcatcaccgg caagctcgtg
     5461 gtcaccgtct cgcagcgcgc gatggtcgac ggcaagccca tcggcgtcgt cggcgccgac
     5521 atgttcatca cgcgcgtcgg cgagatcgtc ggcgccaaga agctctccgc caacggcaag
     5581 acctacctcg tggacaagga cggcctcctc atcacccacg aggatcccga gaagatcctc
     5641 aaggccaacc tcttcgaggg caacatcctc tcgccgctca aggacaagat cctcaagggc
     5701 ggggagacca ccttcggcat cttgcggaag gagggcgcct actacgcctc catccgcatc
     5761 cccgagctca acgcgatcct cgtcaccgcc ggacccgtgt cggacatcta cggcgccctg
     5821 aacgccttcc tggcgcggct cgccctcatc gtcggcctgg gcatcgcggt ggccgtcctc
     5881 gtcctcttcg ccatcgcgag gagcttcacg aggcccatcc tcgccctcac cgccgtctcc
     5941 aagcgcctct ccgaggggcg cctggacttc gacgccgcga aggaaggcct ggcgggccgc
     6001 aacgacgagc tcggagagct ctccgaggcc ttcggcctca tggtcctccg cgtcgcggag
     6061 gtcgtggacg aggtgaagag ctccgtggag gtcctcctcc gcggctcctc gaacctgaac
     6121 caggcgagcg tcgcgatgtc ccagggcgcc accgagcagg cggcgagcac cgaggaggtc
     6181 tccgcctccc tggagcagat gggcggcaat atcaggaaca acgcggacaa cgcgaaggag
     6241 acggagcgca tctcccgcaa ggcggcgaag gacacggagg agggctccaa ggcggtgctc
     6301 gagaccgtcg gggcgatgaa ggacatctcc cagaagatcc tcatcatcga ggaaatcgcc
     6361 cgccagacga acctcctggc cctgaacgcg gccatcgagg cggcgcgcgc gggcgaggcc
     6421 ggcaagggct tcgccgtcgt cgcgagcgag gtgcgcaagc tcgccgagcg ctcgcagaag
     6481 gcggcgacgg agatcggcga gctctccgtg aagagcgtcg gcgtatcgga gaaggcgggc
     6541 tcgatgctcg accagatcgt gccggacatc cagaagacct cccagctcgt gcaggagatc
     6601 tccgcggcct ccaacgagca gagccagggc acggaccaga tcaccaaggc catcatgcag
     6661 ctcgacaagg tcgtgcagga gaacgccgcc tccagcgagg agatcgcggg catgaccgag
     6721 gagatcgcgc gcctggccca gaacctctcc gcgaggatct ccttcttcaa gacgaagggc
     6781 ggcagggccg agggcgccgc ccaggcggcc gcccaagact cgggccgcgg gcgtcccaag
     6841 gcccagtccg ccggccaaac ctccgctccg cgggggaagc cggccgaggg ccgctccgat
     6901 cccaagcctg atcccaggcc cgcgcccaag gcgcagatca ggccgagccg cgagaccggc
     6961 atcacgatca agtcgggcgc cgaggacgag gacttcgagg agttctaggc catacggccc
     7021 gacaaacgcg tcagaatgaa gagccgcccc ccgcaagccg gggggcggct ttgtttgcgc
     7081 tgctcctgga tgagcttagg cggcgcctgc ctcgggcggc ggtgccggac tgcgcctaga
     7141 cgggctcggg cgagccctct ccgtcggccc tggccgaggc ctcggccagg gcctcgcgcc
     7201 tcgccctccc ccgcccctgc accttcagga tgaggatcga gagctcgtag agcccgagca
     7261 tcggcaatgc gaggagtacc tgggagacga cgtcgggcgg cgtgaggatg gcggccgcga
     7321 tgaagatgcc gaggacggcg acgctccgcc ccctcacgag gagctcggcc ttgaggaggc
     7381 cgaaggcgcc caggagggcc gccgccatgg gaagctcgaa ggcggccccg aagcccagga
     7441 ctaggcccga gacgaagtcg aagtagtcct tgatcgcgaa gaccgcctcc accctcgggt
     7501 tcgagtagga gaggaagaac ttgatcgtga agggcaggat cacgtagtag gcgaaggccg
     7561 cgccggcgac gaagaagaag gccccgaaga agaggcagag gaagaccgag ccgcgctcgc
     7621 gcctggagag ggccgggctc acgaagagcc agagctcgaa gaggatgacg gggagggaga
     7681 gggccaggcc cgcgacgagg gcgagcttca cgtaggagac gaagagctcg ggcggcgaga
     7741 ggtagacgaa ctggagggta cccgccggcc tcatcacgaa ttcggcgagc ttctccgcga
     7801 aggagaagca gccgaagccc gcggccagga aggccgcgca ggagacgacg agccgcttgc
     7861 ggagctccgc tagatggtcc gtgaaggtca tgggtcctgc ctgacgtgtc gggatgggga
     7921 agggcggcgc ggagccccgg ccccggggcc gtgccggcgc ccgccttcag gccgggcctg
     7981 tcggcggcgc ctttcggtgg agtccttcgc cgggccccaa ggccggagca tcgcgcgggc
     8041 cttgggcttt aggccttctt ggccttcgac ttggcggcct cgggggcctc ggcgggtttc
     8101 gcgaccgcct cgatcctcgg agcctgggcc tcagagccgg cgtcggcgtc ggtcgccttc
     8161 gcgatgtcct cggtcacctt gtccgagtgc ttcttgaact ccctgatggc ctggcccaca
     8221 gccttgccga tctcgggcag cttcttgggc ccgaagatga cgagggcgat gacgaggacg
     8281 agtatgagct cagcgggtcc gattcttccg aacatggttc actccttggc ttcgggccgg
     8341 acgagctccg gcctcgtata gacgatggga ctgggggtcc cgatccttcc gatcaaagta
     8401 attccggctt cccgcgcgaa ggcgtaggcc tccgtggtgg ggatgctccg ggatacgagg
     8461 acgggcaggc cggccttggc ggccttctgc gccatgtccg ccgcgatccg gccgctcgtg
     8521 aggaggacgg agcgggagaa atccgccccg tccaggaggc cgcggcccag gaccttgtcg
     8581 acggcgttgt gcc
//
LOCUS       DAOOSJ010000104         8582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5200_length_8582_cov_7.051366, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000104 DAOOSJ010000000
VERSION     DAOOSJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5200_length_8582_cov_7.051366"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLG14_06500"
     CDS             <1..723
                     /locus_tag="PLG14_06500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOX48824.1"
                     /translation="PGSELAKAGVNTAILSTVIGQTLRLPAYKLSYLATGALLHDVGM
                     LRIPDSIVKKRGGLTEEEAQKIRAHPLLSYRISTKELLYPDDVGVVGLQHHERWDGEG
                     YPRKTAGAEIDALARIVSVADAFEAMVSVKPYRNSMIGYAAMKTLLSDNSRRFDPEIL
                     KAFIKSMGIYPLGSTVLLNNAAIARVVETHPDAPLRPKLRLLVDEFGNRAEGERGEVV
                     DLLLEKTLFIARALDPKELAGD"
     gene            728..1255
                     /locus_tag="PLG14_06505"
     CDS             728..1255
                     /locus_tag="PLG14_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOX48825.1"
                     /translation="MAEGPKGGGGLPDSPPEQAAAPGPAPASALPGGAAPAGADPGRA
                     VPAGARPVGPAPGASAAGKGRAGEAAAALVLEAEGYRLLARNFRGPAGEIDIVALRGD
                     TIAFVEVKSWSALGLEDLADSIGARKRRRIVETSKIFLARNRQYSSMRARYDVLLMRD
                     GIVARRIESAFTGDL"
     gene            1394..2089
                     /locus_tag="PLG14_06510"
     CDS             1394..2089
                     /locus_tag="PLG14_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48826.1"
                     /translation="MAEERRDRGGQPGRGGRPGDQRQSQQGQPGRAPSGQGRNGGQPE
                     GRGPSAKEGRRFGRRRSGRPGPREDRARDEKAKTDRAREDRAKAEPSRDDRAREDRAR
                     EERASEERAREERARAYAAAEPEPSSPESLGNVDCPLCGKPVYDLTTAVCADKESGAP
                     AHFDCVLERVAAAETLGPAERLVYLGSGSFGVVEFKDKSESAFVVKRRVQWEKEGDKK
                     PWRRGLSTRITRI"
     gene            2151..3113
                     /locus_tag="PLG14_06515"
     CDS             2151..3113
                     /locus_tag="PLG14_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOX48827.1"
                     /translation="MPVPRIYFSRCLGFAACRWNGERLRDEFVERLASRVEVLHDCPE
                     MGIGLGCPRDPIRAVKEGGTVDLYQPATGRSVGAEMRAWSSSLLDSLPAVDGFLLKSR
                     SPSCGWKDAKVYNSRKPDAGSAPGAGLFGAAVLAARPGVPVEDEGRLLNFTLRERFLA
                     AVWTLARFREVEASGDMGALVDFHSKNKLLLLLYNQREMRGLGRIVANPERRRTAEVL
                     AVYRPGLERALSAPPKYSSLVNAIEHAFGGFSESLSPGERALFMNTVEEYRDERVPAS
                     VLLRLVTAWALRFGNRYLLEQTIFEPYPLELVDITDSGKGRSGR"
     gene            complement(3138..4940)
                     /locus_tag="PLG14_06520"
     CDS             complement(3138..4940)
                     /locus_tag="PLG14_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48828.1"
                     /translation="MGFVLDGLESEAYDRKYSDRELFGRIGAYFRPWRARIAAVGAVL
                     ALASLSEFLAQVVIAKALDAAAARPEPAVFALLALAIGGFGSLAWLFNYLRHYLSGRV
                     VGETVYRLRSDAFSRAVAHDLSFYDEESSGKVVSRVTTDTEDFAGVVGLVVDFLSQVV
                     LIGVFFAYLLSLSPRLALLLLAMAPLAGGLALSFRSLARFVTRNAKRVTARVNARIQE
                     SIAGIGVAKGFRREKALWRDFKEDNALSYRFHLRRGVVLQLIFPVVSLAAGLGTGLVS
                     YSGGLAVRSGALSLGEWFLFMQAVGFFWWPLLNIASFWSQLQDGLAAAERVFSLIDRE
                     PRVAQAGASAPARIEGEVEFRSLDFSYSSKEAVLEGFSLRIPAGEKLAIVGRTGAGKS
                     SIVRLLCRFYEFQGGELLVDGRDIRGYSLDALRGAIGLVAQDPFLFPGSVADNIRYSR
                     PEADDAEVERAARSLGRGDWVDDLPRGLETETGHRGSSLSMGQRQLVVLARVLLKDPR
                     IFVLDEATSSVDPFTEAQIQEGLESLMEGRTAIVIAHRLSTVRFADRIVVLEKGRIVE
                     EGSHEALLAKGGDYAGLYETYFRHQSLEYIETRR"
     gene            complement(4944..6794)
                     /locus_tag="PLG14_06525"
     CDS             complement(4944..6794)
                     /locus_tag="PLG14_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48829.1"
                     /translation="MKEELSEFRSGEERSYDLRGPRRWIFSHLLRYPWLPALAFLFSV
                     GNNVGYSGIQVLVGKAFDLLAKPGSGADGLAAVALLIAGASALQGLAGVGRNLSFELL
                     AQRIERDARAELFSSLLGKSQTFHSRQRLGDLMARATNDVHFLNLMFSPGLAMIMDSA
                     LTMVVPLFVIASIEPRLLLVPAVFALLLCLTVWEYNRRLAPATDEQRERFGELNAALA
                     DALEGIETVKANLGEARETARFAKGAGRLRDLYIRVARIEGRYWPLLVFALCWGAAFF
                     HALLLWRAGALSLGEVVGYMLLFNAFRFTTFISIFSFNLFQMGLSSAARVLETIVART
                     DLDENPRGFSKRIEGRVEFRGVSFSFCGPSGCEAAAAFAAGPGGGSAGGGSASPLVLK
                     NLSFVVEPGQTVAIVGRTGSGKTSLARLVNRVFDADEGQVLVDGVDVREWNLESLRSQ
                     IASIEQDVFLFSRSIRDNIAFGRDGPGGDGAFPDAEIEAAAREAQAHDFVSGFEKGYA
                     TEVGERGVTLSGGQKQRIAIARAFLVDPRILILDDSTSAVDSRTEDEIQKAMRRASAG
                     RTTFLITHRLSQIRWADRILLLKGGELAASGTHAELLEKSEDYRRLFART"
     gene            7347..7670
                     /locus_tag="PLG14_06530"
     CDS             7347..7670
                     /locus_tag="PLG14_06530"
                     /inference="COORDINATES: protein motif:HMM:NF040785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HOX48830.1"
                     /translation="MSYLNADSLLPPDLLREVQKYIQGSLIYIPRPESLRLGWGSKSG
                     AREALDRRNAEIRAAKAAGRSIDELADEYALSPDGIRKVLYRNRGPLIAEIRPVDEEP
                     AEVSA"
     gene            complement(7832..8329)
                     /locus_tag="PLG14_06535"
     CDS             complement(7832..8329)
                     /locus_tag="PLG14_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOX48831.1"
                     /translation="MKKDLSGQAPLREHLLLHLSGKAAHIPFSAAIKGFPSALAGERV
                     PRLEHSAWGLVFHLRLCQRDILDYALKEGTVSPEYPSGLWPKEAGPASQAEWKRVIAG
                     FEADLAEIEAMVGDPGRDLFAPMRPGLDESLLEMASLVIDHNSYHIGQLVDLRMLLGV
                     PVRDW"
     gene            complement(8375..>8582)
                     /locus_tag="PLG14_06540"
     CDS             complement(8375..>8582)
                     /locus_tag="PLG14_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48832.1"
                     /translation="QGLRVSLVVNIAAATVGAAVGAGGLGVPIVSGLRAFDPLLILSG
                     SLPVAFLALFADSALRAVEETIGA"
BASE COUNT         1376 a         2979 c         3033 g         1194 t
ORIGIN      
        1 ccgggcagcg agctcgccaa ggcgggcgtc aacaccgcca tcctctccac ggtgatcggt
       61 cagaccctga ggctccccgc ctacaagctc tcctacctcg ccacgggagc ccttctccac
      121 gacgtgggca tgctccgcat ccccgactcg atcgtgaaga agcgcggggg gctcacggag
      181 gaggaggccc agaagatccg cgcccatccc ctcctctcct accgtatttc cacgaaggag
      241 ctcctctatc cggacgacgt gggcgtcgtg ggcctccagc accacgagcg ctgggacggc
      301 gagggctatc cccggaagac ggccggcgcg gagatcgacg ccctggcccg catcgtttcg
      361 gtggccgacg ccttcgaggc tatggtcagc gtgaagccct accgcaactc gatgatcggc
      421 tacgccgcga tgaagaccct cctctccgac aactcgaggc gcttcgatcc cgagatcctc
      481 aaggccttca tcaagtcgat gggcatctac cccctgggct cgaccgtcct cctcaacaac
      541 gccgcgatag cgcgggtcgt ggagacccat ccggacgccc ccttgcggcc caagctcagg
      601 ctcctcgtcg acgaattcgg caaccgggcg gagggagagc gcggcgaagt cgtggacctc
      661 ctcctcgaga agaccctctt catcgcccgc gccctggatc ccaaggagct cgccggggac
      721 tagggcggtg gctgagggcc cgaagggcgg cggggggctc ccggacagcc cgcccgagca
      781 ggccgcggcg cccggacctg cgcccgcgag cgccttgccg ggcggcgccg caccagccgg
      841 cgccgatccc ggccgcgcag tccctgccgg cgcccggccc gtcggccccg cgccgggcgc
      901 ctcggcggcg gggaaggggc gggcgggcga ggcggccgcc gccctggtcc tcgaggccga
      961 gggctaccgc ctcctggccc gtaatttccg cggcccggcc ggcgagatag acatcgtggc
     1021 cctccgggga gacacgattg ccttcgtcga ggtaaaatca tggtcggccc tgggccttga
     1081 ggacttggcc gactcgatcg gcgccaggaa gcggcgccgg atcgtcgaaa cgtctaaaat
     1141 cttcctcgcc cgcaatcgac aatatagtag catgcgggcg cgctacgacg tcctgctcat
     1201 gcgcgacggc atcgtcgctc gtcgcatcga atcggcgttt acgggagacc tatgacgggc
     1261 cgaggagcca gtcggtccga ccaagctcgg atcgaggaac taaaagccaa gctgcgcgac
     1321 gaggcctata tggccggagc gatcctcagg atcgcgacgg tgctgagcgc gcggctcacg
     1381 gaaggatacg ctgatggcgg aagaaagacg cgataggggc ggccagcccg gcaggggagg
     1441 gcgcccgggc gaccagcgcc agagccagca ggggcaaccg ggccgcgcgc cctccggcca
     1501 gggccgcaac ggcggacagc ccgagggccg cggaccttcc gcgaaggaag gccgccgctt
     1561 cggccgcagg cgctccggcc gcccgggacc gcgggaagac cgcgccaggg acgagaaggc
     1621 caagaccgac cgcgcccggg aagaccgcgc caaggcggag ccctcgcggg acgaccgcgc
     1681 gagggaggac cgcgcccgcg aggagcgcgc gagcgaggag agggccaggg aagagcgggc
     1741 ccgcgcctac gccgccgccg agcccgagcc ttcatcgccc gagagcctcg ggaacgtcga
     1801 ctgcccgctg tgcgggaaac ccgtctacga cctcaccacg gccgtctgcg ccgacaagga
     1861 atcgggcgcc cccgcccatt tcgactgcgt cctggagcgg gtagccgccg ccgagaccct
     1921 gggccccgcc gagcgcctcg tctacctcgg ctcgggctcc ttcggcgtcg tcgagttcaa
     1981 ggacaagagc gagagcgcct tcgtcgtcaa gcgccgcgtc cagtgggaga aggaagggga
     2041 caagaagccc tggcgcaggg gcctctccac ccgcatcacc aggatctagc cccccgggcg
     2101 cgggtgagcg cgccgggatc gggagagagc gtgtcccttg aggaggcgcc atgcctgttc
     2161 ccaggatcta tttctcccgc tgcctcggct tcgcggcctg ccgctggaac ggcgagaggc
     2221 tccgcgacga gttcgtcgag cggctcgcct cccgcgtcga ggtcctgcac gactgtcccg
     2281 agatgggcat cggcctcggc tgtccccgcg acccgatccg cgcggtgaag gagggcggga
     2341 ccgtcgacct ctaccagccc gcgacgggaa ggtcggtcgg ggccgagatg cgcgcctgga
     2401 gctccagcct ccttgactcc ctgcccgcgg tcgacggctt cctcctcaag tcccgttccc
     2461 cttcctgcgg ctggaaggac gccaaggtct acaactcgag gaaaccagac gcaggctcgg
     2521 ccccgggcgc gggcctcttc ggggcggcgg tcctcgcggc gcggcccggc gtccccgtgg
     2581 aggacgaggg ccggctcctc aacttcaccc tgcgcgagcg cttcctcgcg gccgtctgga
     2641 ccctcgcgcg gttccgcgag gtggaggcct cgggcgacat gggagccctc gtcgacttcc
     2701 actcgaagaa caagctcctc ctcctcctct acaaccagcg cgagatgcgc gggctcggaa
     2761 ggatcgtcgc caatccggag cggcggcgga cggccgaggt cctcgccgtc tacaggcccg
     2821 ggctcgagcg ggccctctcg gccccgccca agtactcgag cctcgtcaac gccatcgagc
     2881 acgccttcgg gggcttctcc gagtcgctct cgcccgggga gcgggccctc ttcatgaaca
     2941 cggtggagga ataccgggac gagcgcgtgc ccgcgagcgt cctcctcagg ctcgtgacgg
     3001 cctgggccct gcgcttcggg aaccgctacc tcctcgagca gacgatcttc gagccctatc
     3061 ccctcgagct cgtggacatc acggactcgg gaaagggccg ctcgggccgc taggcggccg
     3121 cctggcgccc gccgccctca gcgccgcgtc tcgatgtact cgaggctctg gtgccggaag
     3181 taggtctcgt agaggcccgc gtagtcgccg cccttggcga gcagggcctc gtggctcccc
     3241 tcctcgacga tcctgccctt ctccaggacg acgatgcggt cggcgaagcg gacggtggag
     3301 aggcgatgcg cgatcacgat cgcggtgcgg ccctccatga gcgactcgag gccctcctgg
     3361 atctgcgcct cggtgaaggg gtccacgctg gaggtcgcct cgtccaggac gaagatccgc
     3421 gggtccttga ggaggacgcg ggccaggacg acgagctggc gctggcccat ggagagggag
     3481 gagccgcgat ggccggtctc cgtctcgagg cctctcggca ggtcgtccac ccagtcgccg
     3541 cggcccaggg acctggccgc ccgctccacc tccgcgtcgt ccgcctccgg ccgcgagtag
     3601 cggatgttgt ccgcgacgct gcccgggaag aggaaggggt cctgcgccac gaggccgatg
     3661 gcgccgcgga gcgcgtccag ggagtagccg cggatgtcgc ggccgtccac gaggagctcc
     3721 ccgccctgga actcgtagaa gcggcagagg aggcgcacga tggaggactt cccggcgccc
     3781 gtgcggccga cgatcgcgag cttctccccg gcggggatcc gcagggagaa gccctcgagg
     3841 acggcctcct tcgaggagta ggagaagtcc agggagcgga actcgacctc gccctcgatc
     3901 cgcgcggggg cggaggcgcc ggcctgcgcg acgcggggct cccggtcgat gagggagaac
     3961 acgcgctcgg ccgccgcgag gccgtcctgc agctggctcc agaaggaggc gatgttgagg
     4021 aggggccacc agaagaagcc gacggcctgc atgaagagga accactcgcc caggctcaag
     4081 gccccgctcc ggacggcgag gccgcccgag taggagacga ggcccgtgcc caggcccgcc
     4141 gcgaggctca cgacggggaa gatgagctgg aggacgacgc cgcgcctcaa gtggaagcgg
     4201 taggagaggg cgttgtcctc cttgaagtcg cgccagagcg ccttctcgcg cctgaagccc
     4261 ttggccacgc cgatgcccgc gatcgactcc tggatgcgcg cgttcacgcg ggccgtgacc
     4321 cgcttcgcgt tcctcgtgac gaagcgcgcg agggagcgga aggagagggc gagtcccccc
     4381 gcgaggggcg ccatcgcgag gaggaggagg gcgagccggg ggctcaggga gagtaggtag
     4441 gcgaagaaga ccccgatgag gacgacctgg gagaggaagt cgacgaccag gcccacgacg
     4501 cccgcgaagt cctcggtgtc cgtcgtcacg cggctcacga ccttgccgga ggactcctcg
     4561 tcgtagaagg acaggtcgtg ggccaccgcg cgggagaagg cgtcggatcg caggcggtag
     4621 accgtctcgc ccacgacgcg gccggagagg tagtggcgga ggtagttgaa gagccatgcc
     4681 agggagccga agccgcctat cgcgagggcc agcagggcga agaccgcggg ctccggcctc
     4741 gcggccgccg cgtccagggc cttcgctatc accacctggg ccaggaactc cgagagcgag
     4801 gcgagggcga gcaccgcgcc caccgccgcg atccgggccc tccagggcct gaagtaggcg
     4861 ccgatgcgcc cgaagagctc gcggtccgag tacttccggt cgtaggcctc cgactcgagg
     4921 ccgtccagca cgaatcccat tccttaggtc ctcgcgaaga gccggcggta gtcctcgctt
     4981 ttctcgagga gctccgcgtg ggtgccggaa gccgcgagtt cccctccctt gaggaggagg
     5041 atgcggtcgg cccagcgtat ctgcgacagc cggtgcgtga tgaggaaggt cgtgcggccc
     5101 gcgctcgcgc ggcgcatcgc cttctggatc tcgtcctccg tcctcgagtc gaccgccgag
     5161 gtcgagtcgt cgaggatgag gatccgcgga tcgacgagga aggccctggc gatcgcgatc
     5221 cgctgcttct ggccgcccga gagcgtgacg ccccgctcgc ccacctccgt cgcgtagccc
     5281 ttctcgaagc ccgagacgaa gtcgtgggcc tgggcctccc tcgcggcggc ctcgatctcc
     5341 gcgtcgggga aggcgccgtc ccctcccggg ccgtcgcgcc cgaaggcgat gttgtcgcgg
     5401 atcgagcggg agaagaggaa tacgtcctgc tcgatgctcg cgatctggga gcggaggctc
     5461 tccaggttcc actcgcgcac gtcgaccccg tccacgagga cctgcccctc gtcggcgtcg
     5521 aagacgcggt tcacgagcct cgcgaggctc gtcttgcccg agcccgtgcg gccgacgatc
     5581 gcgacggtct ggccaggctc cacgacgaag gagaggttct tgaggacgag gggggaggcc
     5641 gaaccgccgc ccgccgagcc accgcccggt ccggcggcga aggccgcggc tgcctcgcag
     5701 cccgagggcc cgcagaagga gaaggagacg ccgcggaatt ccacgcggcc ctcgatccgc
     5761 ttcgagaacc cccgcgggtt ctcgtccagg tcggtcctgg cgacgatcgt ctccaggacg
     5821 cgggcggcgc tggagaggcc catctggaag aggttgaagg agaagatgga gatgaaggtc
     5881 gtgaagcgga aggcgttgaa gaggagcatg tagcccacga cctcgcccag gctcaaggcg
     5941 cccgcgcgcc agaggaggag ggcgtggaag aaggccgcgc cccagcagag cgcgaagacg
     6001 aggaggggcc agtagcgtcc ctcgatcctg gccacgcgga tgtagaggtc gcggaggcgg
     6061 cccgcgcctt tcgcgaaacg cgccgtctcc ctcgcctcgc ccaggttggc cttcacggtc
     6121 tcgatgccct ccagggcgtc cgcgagggcg gcgttgagct cgccgaagcg ctcacgctgc
     6181 tcgtccgtgg cgggggcgag gcggcggttg tactcccaga cggtgaggca gaggaggagg
     6241 gcgaagaccg cgggcacgag gaggaggcgc ggctcgatgg aggcgatgac gaagaggggg
     6301 acgaccatcg tcagggccga gtccatgatc atcgcgagcc ccggggagaa catgaggttg
     6361 aggaagtgga cgtcgttcgt cgccctcgcc atgaggtcgc cgagcctttg ccgcgagtgg
     6421 aaggtctggc tcttcccgag gaggctcgag aagagctctg ccctggcgtc gcgctcgatc
     6481 ctctgggcca ggagctcgaa ggagaggttg cggcccacgc ccgcgaggcc ctgcagggcc
     6541 gaggctcccg cgatgaggag ggcgaccgcc gcgagcccgt ccgcgccgga gcctggcttt
     6601 gcgaggaggt cgaaggcttt gcccaccagg acctggatgc ccgagtagcc cacgttgttc
     6661 ccgacggaga agaggaaggc cagggcgggc agccagggat agcggaggag gtgggagaat
     6721 atccagcgcc tggggccgcg gaggtcgtag ctcctctcct cgcccgacct gaactcggaa
     6781 agctcttctt tcatggttcc cccaattcta ggcgagagcg ggccgcggcg ccaggagccg
     6841 tcaagaaacg aggcggccgg tcccatggga ccggccgcgg tatccgtgtt ctccacggcg
     6901 tgggtctaga gggccatggc cctcttcgct agttcaatca tacgcacctc cttcgccgtc
     6961 cccgatgcct cgggggccgc agtgggttct tggccggctc gacgccacta tggcccatca
     7021 aggccgcttg ggaaaagcga aaattcgctt cagtacgttc tttcccgccg cctcccctga
     7081 acagggtaat gaatctttct cgcgattctg tccgtttaca agaaaccgaa gctcgtgtag
     7141 tattctccac atcttgcatc cgaggaggcc ccaatgagct acatcagcat gaactgcccc
     7201 gcagcctttg cccttgccgc gagcgccgtc gccgcctaag cctcccggag cgcatctact
     7261 catcgtagcg cgttcctcgc ccggcgggcc ttgaagcccc ctggccttag gcggatgcaa
     7321 ccccttgctt cccctgagga acgaacatgt cgtatttaaa cgccgattcc ctgcttcccc
     7381 ccgacctgct tcgggaggtg cagaagtata tccagggttc cctgatctac atcccccggc
     7441 ccgagtcgct caggctcggc tggggctcga agagcggggc ccgcgaagcg ctcgaccggc
     7501 gcaacgccga gatccgggcc gcgaaggcgg ccggcaggag catcgacgag ctcgccgacg
     7561 agtacgccct ctcgcccgac ggcatcagga aggtcctgta ccggaaccgc ggtcccctga
     7621 tcgcggagat ccggcccgtc gacgaggagc ccgccgaggt ctcggcttga ggcctcgttt
     7681 cccggccccg gtcctagccc gctaggaccg ggggccgggc ccctggcggt ccagcccctg
     7741 tccgcgttct ctccgccttg gctccgccaa tcgccacctc accccgcgct acctttcccc
     7801 gcgccacctc tcgccgaggc ctccctcgtc ctcaccaatc cctgaccggc accccgagga
     7861 gcatgcggag gtcgacgagc tggccgatat ggtaggagtt gtggtcgatg accaggctgg
     7921 ccatctcgag gaggctctcg tccaggcccg gccgcatcgg cgcgaagagg tcgcgcccgg
     7981 gatcgcccac catggcctcg atctccgcga ggtcggcttc gaagccggct atcacgcgct
     8041 tccactccgc ctgggaggcg gggcccgcct ccttgggcca gagaccggag gggtactcgg
     8101 gcgagacggt cccctccttg agcgcgtagt cgaggatgtc cctctggcag agccgcaggt
     8161 ggaagacgag gccccaggcc gagtgctcga gcctgggcac ccgttcgccc gcgagggcgg
     8221 agggaaagcc cttgatcgcg gccgagaagg ggatgtgcgc ggccttcccc gagaggtgga
     8281 ggaggaggtg ctcccgcagg ggggcctggc cggaaaggtc cttcttcatc gcgcggctcc
     8341 tttcgggcgc ccttccggcg ccccgcggcc gatgctaggc gccgatggtc tcctcgaccg
     8401 cccgcagggc cgagtcggcg aagagggcga ggaaggccac gggcagggat cccgagagta
     8461 tgaggagggg gtcgaaggcg cggagccccg agacgatggg gacgcccagg cccccggcgc
     8521 cgacggcggc gcccaccgtc gccgccgcga tgtttacgac gagggagacc ctgaggccct
     8581 gg
//
LOCUS       DAOOSJ010000105         8528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5235_length_8528_cov_6.692789, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000105 DAOOSJ010000000
VERSION     DAOOSJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5235_length_8528_cov_6.692789"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1372
                     /locus_tag="PLG14_06545"
     CDS             413..1372
                     /locus_tag="PLG14_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOX48833.1"
                     /translation="MEKPRLVVGKGSKAGFRTVQAAVDAAPESGGAVIAIGPGEYREK
                     LFVGKPGLRLEGAGRDRTVLRWDDAAEKPFGDGTKTGTFRSYAAYFGGDDFEAADLSV
                     VNGAGRGPGVGQAIAAYVDSRRARFSRCAFRGRQDTLFLGPLPPRPRVPGSFVGPGES
                     GPRILKAHRFEDCLVEGDVDFIFGSAAALFLRCEIRSLRLPPGMKGYVTASSSPEGAA
                     LGLCFLDCGLSGDAEPGSVFLGRPWRPHAQAAFLYCSLGAHIAPRGWSDWEEPSDRGS
                     VGFVEGGSAGPGAGTDSRAAWASVLGESETEAFARSLLASFGA"
     gene            1504..2502
                     /locus_tag="PLG14_06550"
     CDS             1504..2502
                     /locus_tag="PLG14_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48834.1"
                     /translation="MKLSDDRKSAILLAFLVLLVLSAAAYSLARTRLQSAWTERKIEG
                     VGFLVEKSAVAGGRLDFEAAAKEAFGTLRAALGDEPARFLPPDLLVVAAYGGYGAVSY
                     ADARLERAFRGRPTAILLVEPLRSKYAPNEPLAAGRTLRDERIALLDLYVAERNGDTA
                     AGALVHALSDFIVEWNVPAALRSRVDPGALHSPEARRELYLAREAFSFYLGQRGRLQA
                     EGAPAIGASVGPAGKPALGPAAAPGKEAELLAERYRGLLDQRYAPEGPVFGGGNLRAR
                     FAPEAAEVELLVENHYAAAAAAGREGEANLLAAFRAMASGGYEDPRIPAAAAGASF"
     gene            2579..3226
                     /locus_tag="PLG14_06555"
     CDS             2579..3226
                     /locus_tag="PLG14_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOX48835.1"
                     /translation="MAFDLRSALYSLPAIVLGLTAHEYCHALAAYRLGDPTAKEEGRL
                     SLNPIRHIDPLGFLFLVVAGFGWARPVRFSRERLVRPRRDEALIALAGPTSNLILALA
                     GSLVLRLADLAFPSFLGAPGTVGVAVFQLLLYFVYINYGLFVFNMIPLPPLDGSHLVF
                     SAFPIKPETEAKLYRYGSFALLAVILVENRTGMDLLPIGRMVRGLAGFVFRVLGF"
     gene            3257..3862
                     /locus_tag="PLG14_06560"
     CDS             3257..3862
                     /locus_tag="PLG14_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOX48836.1"
                     /translation="MEQTFLSATILLILITDPLGNIPPLLALLRNVEPRRRTRIILRE
                     CAIAFVVLLVFLLFGRQILGVLHLSEDTLRIAGGVILFLIALNMIFPGSGARMTDAPE
                     GGEPFIVPLAIPLISGPSAMVTAMLISGNDPSRMLQWVGALVITIGTSATVFLLANRM
                     QKVLGPHVIAAFERLMGLVLAAISIDMLLKGLESYLKGALG"
     gene            complement(3964..4572)
                     /locus_tag="PLG14_06565"
     CDS             complement(3964..4572)
                     /locus_tag="PLG14_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HOX48837.1"
                     /translation="MERGKGLSREGAVEAYLALLDEGVPANFRGLAAKLGCAHTNLYN
                     FFADWDALRWAAIAEILRRLVAAMEGGAAEGGEGAGRKAVRRAEAEVQAALRAYVDFG
                     LGHPSWYRLVWLEPLASPMPADVRSLSDEAQAAVFARLARALPGSGGEGLKTAVACGH
                     DLVHGALAKLLSGREPAPPDAAAFSARLADLALAAMAAAARA"
     gene            complement(4572..5459)
                     /locus_tag="PLG14_06570"
     CDS             complement(4572..5459)
                     /locus_tag="PLG14_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48838.1"
                     /translation="MLATYPRSLYLTGSLLALSMAATALPGLLVPGFYEPFLTRYGLA
                     YAQVFMQDLATVLLLPILAWSMLAARRGSARGVAVWGGVCVYALYYYSFYGFDKLYTP
                     LYPLYLAVMGLGAWSLVGLLAGTDAKAFAALADARLPVRFCAAVLAVPLLFVPIWLSM
                     LFAGIAAKKAAPAATVFPLDLCFLIPAAAWAAVALWRRRPIGYLAGGILLVKAVISGV
                     ILAGGTLYQLLAGEKANPGELALYLVLALPGAAALGLFLKRLGPAEKGAAPGAAAPGA
                     TPPAAPGTATHGASAPGAR"
     gene            complement(5611..>6038)
                     /locus_tag="PLG14_06575"
     CDS             complement(5611..>6038)
                     /locus_tag="PLG14_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOX48839.1"
                     /translation="YEGKVLLVVNTASKCGFTPQYEGLEALHRKYKERGLVVLGFPCD
                     QFAHQEPAGDAEIAAFCKLNFGVTFPLMAKVEVNGQGTHPVFAFLKRRAPSLLGSKVK
                     WNFTKFLVDRDGKTVARFAPSMEPVRLEKAIEAALGVEA"
     assembly_gap    6039..6138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6200..6646
                     /locus_tag="PLG14_06580"
     CDS             6200..6646
                     /locus_tag="PLG14_06580"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOX48840.1"
                     /translation="MKEPKTRKTEADPAAFVEAIPDEGRRRDAARLLELMVRVSGERP
                     AMWGGSIVGFGRYAYRYASGREGEWFRIGFAPRARAMSLYLMGLAYKGEELESLLARL
                     GRHELGKGCLYLRRLEDADQAVLEELLRLAWAAPGLGEAPAPGGAD"
     gene            6676..7536
                     /locus_tag="PLG14_06585"
     CDS             6676..7536
                     /locus_tag="PLG14_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX48841.1"
                     /translation="MRNKTGEELAGIGAMAACAFLWSLAGIFIKLIDWQPFAIAGGRS
                     LVAAVFILLWIKKPKFTFSKAQVGAALASAGTMLLFVYANKATTSANAILLQYGAPIY
                     VALLAAPLLGEKPRAEHWAALVAVLGGILLLFMDGLGGGSLAGNLAAVGAGVTFALYV
                     IFMRKQKEGSPLESALLAHVLTAAVALSISLFLPAPRIEPRAVAALLGLGILQIGLAS
                     VLFAYGIKRVRAVESVLLGVIEPVLNPVWVFLATAEAPGPRAILGGGIVIAAVLASSL
                     VSLGRDASRA"
     gene            complement(7546..8436)
                     /locus_tag="PLG14_06590"
     CDS             complement(7546..8436)
                     /locus_tag="PLG14_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX48842.1"
                     /translation="MRTTEGGLAYEERGSGFPFVALHGYTLDRRMSLGAFEPLFDAEG
                     RAALPPSAGAGRPEGGRSSRSYRRIYLDLPCMGESADLEEASSDAMLAATLDAIGELT
                     GGGDFLLAGQSYGGYLARGAARALGPRVAGLFLLCPAIVIRRALRDLPPPAVLRDEEG
                     WAAGAAEEEIEGFKECSVARTRYTFERTRAEILSGIGVARMGLLERIISEGFAFSFDR
                     PAAYPEGAFDARFERPACFVLGRQDSTVGWKDALALAGRYPRASFHVLDAAGHNLQLE
                     QAGLFASAFGAWIGACEAWA"
BASE COUNT         1136 a         3103 c         2968 g         1221 t
ORIGIN      
        1 gctggagagg taacgcgggg ccgatccgct ctccggcatc cgcgcgcata cgcgcgcgga
       61 tgccggtagc ctcgtgtgcg gccgtgcgaa gccacggata gtctaccgaa agctggagcg
      121 gtagaaggcg gcctgtccgc tctccggttc cccgcgcctc cgcgcgggga accggctcca
      181 tcaagagcat ctccgcgcgc atacgcgcgc ggatgccggt agcctcgtgt gcggccgtgc
      241 gaagccacgg atagtctacc gcaagctgga gcgttaaaag gcggcctgtc cgctctccgc
      301 cccccgcgcg cataggtgcc ggggggcggt tccgttttcg gaggccctgc ccgcaaggtc
      361 ggcgggcctc gcggcctggc atcccacgcc gcggcgcggg tatactcggg gcatggaaaa
      421 accccggctc gtggtcggca agggaagcaa ggccggtttc cggaccgtgc aggccgcggt
      481 cgacgccgcg cccgagtcgg gcggggccgt catcgccatc ggcccgggcg agtaccgcga
      541 gaagcttttc gtcggcaagc ccggcttgag gctcgaggga gcgggacgcg acaggaccgt
      601 cctccgctgg gacgacgcgg cggagaagcc cttcggcgac gggacgaaga ccggcacctt
      661 ccgctcctac gccgcctatt tcggcggcga cgacttcgag gcggccgacc tctccgtggt
      721 gaacggcgcc ggccgcgggc ccggcgtggg gcaggcgatc gccgcctacg tcgactcgag
      781 gcgcgcccgc ttctcgcgct gcgccttccg cgggcggcag gacacgctgt tcctcggccc
      841 ccttccgccc cggccccgcg ttccgggcag cttcgtcggc ccaggggaat cgggccccag
      901 gatcctcaag gcccaccgct tcgaggactg cctggtcgag ggagacgtcg acttcatctt
      961 cggctcggcc gccgccctct tcctgcgctg cgagatccgc tccctgcggc tcccgccggg
     1021 catgaagggc tatgtcacgg cctcctcgtc gcccgaaggc gccgcgttgg gcctctgttt
     1081 cctggattgc gggctctcag gcgacgccga gcccggctcg gtcttcctcg gccggccctg
     1141 gcggccccac gcccaggccg ccttcctcta ctgctccctg ggcgcgcata tcgcgcccag
     1201 gggctggtcg gactgggagg agccctccga ccggggctcc gtgggcttcg tcgagggagg
     1261 gagcgccggc cccggggccg ggacggactc ccgcgccgcc tgggcctccg tcctcggcga
     1321 gtcggagacc gaggctttcg cgcgctccct cctcgcctcc ttcggcgcct gatcctaggt
     1381 cgaggccgag gccgggcgcg ggctggccgg gcaggggcca ggcgcctgag gatgcgggcg
     1441 ccttccgcct ccgcgcgtta tactcgaagg aggggttccc aagggagccc cggaggcatt
     1501 gccatgaagc tgagcgacga ccggaagagc gccatcctcc tcgcgtttct cgtcctcctc
     1561 gtcctgtccg cggcggccta ttccctggcg cggaccaggc tccagagcgc ctggaccgag
     1621 aggaagatcg agggcgtcgg cttcctcgtg gagaagtcgg cggtcgccgg gggacgcctg
     1681 gatttcgagg cggcggcgaa ggaggccttc gggacgctcc gcgccgccct gggcgacgag
     1741 ccagccaggt tcctcccgcc ggacctcctc gtcgtcgcgg cctacggcgg ctacggcgcg
     1801 gtctcctacg cggacgcgcg gctcgagcgg gccttccgcg ggcggccgac ggccatcctc
     1861 ctcgtggagc ccctccgctc caagtacgcg cccaacgagc ccctcgccgc cggcaggacc
     1921 cttcgggacg aaaggatagc cctcctcgac ctctacgtgg ccgagcggaa cggcgacacc
     1981 gcggccggcg ccctcgtcca cgccctctcg gacttcatcg tcgagtggaa cgtccccgcc
     2041 gccctgcgct cccgcgtcga cccgggcgcc ctccattctc cggaggcgcg gcgcgagctc
     2101 tacctggcgc gcgaggcctt ctccttctat ctcggccagc gcggccgcct ccaggcggaa
     2161 ggcgcgccgg cgatcggcgc ctcggtcggc ccggcgggga agcccgcgct gggtccggcg
     2221 gccgcgccgg gcaaagaggc cgagctcctg gccgagcgct accgcgggct cctagaccag
     2281 cgctacgcgc cggaggggcc ggtcttcggc ggcggcaacc tccgcgcccg cttcgccccc
     2341 gaggccgcgg aggtcgagct cctcgttgag aaccactacg ccgccgcggc cgccgccggc
     2401 cgggaggggg aggcaaacct cctcgcggcc ttccgcgcca tggcctcggg cggctacgag
     2461 gatccgcgga tccccgccgc ggcggcgggc gcgtccttct agggcggcgc cgcggcgact
     2521 cccgggatcc aggcgccgct cccttacctt ccgcgcgcgg aatatgcgat agttcggcat
     2581 ggctttcgac ctgcgatccg ccctctattc gctgcccgcc atcgtcctcg gcctcacggc
     2641 ccacgagtac tgccacgccc tcgccgccta caggctcggc gacccgacgg cgaaggagga
     2701 ggggcggctc agcctgaacc cgatcaggca catcgacccc ctgggcttcc tcttcctcgt
     2761 ggtggccggc ttcggctggg ccaggccagt gcgcttctcg cgcgagcggc tcgtccgtcc
     2821 gcggcgcgac gaggccctca tcgccctggc cgggccgacc tcgaacctca tcctcgccct
     2881 ggccggctcc ctcgtcctga ggctcgccga cctggccttc ccctccttcc tgggagcccc
     2941 gggcaccgtc ggcgtcgccg tcttccagct cctcctctac ttcgtctaca tcaactacgg
     3001 cctcttcgtc ttcaacatga tccccctccc gcccctggac ggctcgcacc tcgtcttcag
     3061 cgccttcccc atcaagccgg agacggaggc caagctctac cgctacggat ccttcgccct
     3121 cctggccgtc atcctggtcg agaataggac gggcatggac ctccttccga taggccggat
     3181 ggtccggggc ttagcgggct tcgtcttcag ggtcctcgga ttctagcggc ggaacgggcc
     3241 gcgcaaggag agcaacgtgg agcagacctt cctctcggcg acgattctcc tcatcctcat
     3301 caccgacccc ttgggcaaca tcccgcccct cctcgccctc ctcaggaacg tggagccgag
     3361 gaggcggacg cgcatcatcc tccgcgagtg cgccatcgcc ttcgtcgtcc tcctcgtctt
     3421 cctgctgttc ggccggcaga tcctcggcgt cctccatcta tcggaggaca ccctgaggat
     3481 cgcgggcggc gtcatccttt tcctcatcgc cctcaacatg atctttccgg gctcaggcgc
     3541 gcggatgacc gacgcccccg agggcggcga gcccttcatc gtccccctcg ccattcccct
     3601 catctcgggg ccctcggcga tggtgacggc catgctcatc tcgggcaacg acccgagccg
     3661 catgctccag tgggtgggag ccctggtgat aacgattggg acgagcgcga cggtcttcct
     3721 cctggccaac cggatgcaga aggtcctcgg cccccacgtg atcgccgcct tcgagcgcct
     3781 catgggcctc gtcctcgccg cgatctcgat cgacatgctc ttgaaggggc tggagagcta
     3841 cctgaagggc gccctgggct gaggagcccg ggcctcgggc gcggcgtctc cgccgggccg
     3901 ccggggcctg cgcgtgggcc gcggggacgc cgcgcgggcg ccgccggggc gcgccggccg
     3961 gtcctaggcc cgggccgccg cggccatcgc cgccagggcg aggtccgcga gcctcgccga
     4021 gaaggcggcc gcgtccggag gggcgggctc cctccccgag agcagcttcg cgagcgcgcc
     4081 gtggacgagg tcgtggccgc aggccacggc ggtcttcagg ccctccccgc ccgaacccgg
     4141 cagcgcgcgc gcgagtcggg cgaagaccgc cgcctgggcc tcgtccgaga gcgacctcac
     4201 gtccgcgggc atgggcgagg cgaggggctc gagccagacg aggcggtacc aggaaggatg
     4261 cccgaggccg aagtccacat aggcgcggag ggcggcctgg acctccgctt cggcgcggcg
     4321 gaccgccttc ctgcccgctc cttcccctcc ctccgccgcg cctccctcca tcgccgctac
     4381 gagccggcgg agaatctccg cgatcgccgc ccagcggagc gcgtcccagt ccgcgaagaa
     4441 gttgtagagg ttggtgtgcg cgcagccgag cttcgcggcc aagccgcgga agttggccgg
     4501 aaccccctcg tcgaggaggg cgagataggc ctcgacggcg ccctccctcg agagtccctt
     4561 gccccgctcc atcagcgcgc gcccggcgcc gaggccccgt gggtcgccgt gccgggggcc
     4621 gccggaggcg tcgcgccggg cgccgccgct ccgggggccg cgcctttttc cgcgggcccc
     4681 aggcgcttga gaaagaggcc gagggccgcg gcgccaggca gggccaggac gaggtagagg
     4741 gcgagctcgc caggattggc cttctccccg gcgaggagct ggtagagcgt ccctcccgcg
     4801 aggatgacgc cggagatcac cgccttcacg aggaggatgc ctcccgccag gtagccgatc
     4861 ggcctcctcc gccagagcgc cacggccgcc caggccgccg cggggatgag gaagcagagg
     4921 tcgaggggaa agaccgtggc cgccggggcg gccttcttcg cggcgatgcc ggcgaagagc
     4981 atggagagcc agatcgggac gaagaggagg ggcacggcga ggacggccgc gcagaagcgg
     5041 acgggcaggc gcgcgtccgc cagcgccgcg aaggccttcg cgtccgtccc ggcgaggagg
     5101 ccgacgaggg accaggcgcc gagtcccatc accgccaggt agaggggata gagcggcgtg
     5161 tagagcttgt cgaagccgta gaaggagtag tagtagagcg cgtagacaca gacgccgccc
     5221 cagacggcga cgcctcgggc cgagccccgg cgcgcggcga gcatggacca ggccaggatg
     5281 gggagcagga ggaccgtcgc caggtcctgc atgaagacct gggcgtaggc gaggccgtag
     5341 cgcgtgagga agggctcgta gaatcccggg acgaggaggc ccggaagggc cgtcgccgcc
     5401 atggagaggg cgagaaggga gcccgtgaga tacagggatc gaggatacgt ggcgagcatg
     5461 tggcctccta tattaccaat ggtaatatag cggcctcgca ccagagcttc aaggagtcga
     5521 cgaaggcggg tcggcccggc gccttcggta cgtcagcccg gcgcgtcggc ccggcgcctc
     5581 cggagcgcag gccagcgccg ccggcggctc ctaggcttcc acgccgaggg cagcctcgat
     5641 ggccttctcc aagcggacgg gctccatgga cggggcgaag cgcgcgacgg tcttcccgtc
     5701 gcggtcgacc aggaacttcg tgaagttcca cttgaccttg gagccgagga ggctcggggc
     5761 gcggcgcttg aggaaggcga agaccgggtg ggttccttgg ccgttcacct cgaccttcgc
     5821 catgaggggg aaggtgacgc cgaagttcag cttgcagaag gccgcgatct ccgcgtcccc
     5881 ggccggctcc tggtgggcga actggtcgca ggggaagccg aggacgacga ggccccgctc
     5941 cttgtacttg cggtgcaggg cctcgaggcc ttcgtactgg ggcgtgaagc cgcacttgct
     6001 cgcggtgttg accacgagga ggaccttccc ctcgtagcnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnga gccggcggcg cttgacccgc cgccccttcg ccgcgaaatt
     6181 ctccttggag gcccgtcgca tgaaagagcc caagaccagg aagaccgagg cggatcccgc
     6241 ggccttcgtc gaggcgattc ccgacgaggg gcggaggcgg gacgccgccc gcctcctcga
     6301 gctcatggtg cgcgtgtcgg gcgagcggcc cgcgatgtgg ggcggctcca tcgtgggctt
     6361 cgggcgctac gcctaccgct acgcaagcgg gcgggaaggg gagtggttcc gcatcggctt
     6421 cgcgccgcgg gcccgggcca tgagcctcta tctcatgggc ctcgcctaca agggcgagga
     6481 gctcgagtct ctcctcgcga ggctcgggcg gcacgagctc gggaagggct gcctctacct
     6541 gcgtcgcctc gaggacgccg accaggccgt ccttgaggag ctcctccgcc tggcctgggc
     6601 cgccccgggc ctgggcgagg ctccggcccc cggcggcgct gactaagtcc cgcttcccgg
     6661 gctatcatcc cggccatgcg caacaaaacg ggcgaggagc tcgccggcat cggggccatg
     6721 gcggcctgcg ccttcctgtg gagcctcgcg ggcatcttca tcaagctcat cgactggcag
     6781 cccttcgcga tagcgggagg gaggagcctc gtcgccgccg tcttcatcct cctctggatc
     6841 aagaagccga agttcacctt ctccaaggcg caggtgggcg cggccctcgc gagcgccggg
     6901 acgatgctcc tcttcgtcta cgcgaacaag gcgacgacct cggccaacgc catcctcctc
     6961 caatacgggg cgcccatcta cgtggccctc ctcgcggcgc ccctcctggg cgagaagccc
     7021 cgcgccgagc actgggcggc cctggtcgcc gtcctgggcg gcatcctcct cctcttcatg
     7081 gacggcctgg gcgggggcag cctcgctggc aacctggccg ccgtgggcgc gggagtgacc
     7141 ttcgccctct acgtgatctt catgcgcaag cagaaggagg gctcgcccct ggagtcggcc
     7201 ctcctcgccc atgtcctcac cgccgccgtg gcgctctcga tctccctctt cctccccgcg
     7261 ccccggatcg agccccgggc ggtcgcggcc ctcctcggcc tcgggatact ccagatcggc
     7321 ctcgcctccg tcctcttcgc ctacggcatc aagcgggtgc gggcggtgga gagcgtcctg
     7381 ttgggcgtca tcgagcccgt cctcaacccc gtctgggtct tcctcgcgac ggcggaggcg
     7441 cccggtcccc gggccatcct gggcggcggc atcgtcatcg ccgccgtcct cgcctcctcc
     7501 ctggtctcgc tcgggcggga cgcctcccgg gcctaggtcc taggctcagg cccaggcctc
     7561 gcaggccccg atccaggcgc cgaaggccga ggcgaagagc ccggcctgct caagctggag
     7621 gttgtggccg gccgcgtcca ggacgtggaa gctcgcccgc gggtagcgtc cggcgagcgc
     7681 gagggcgtcc ttccacccga ccgtggaatc ctggcggccc aggacgaagc aggccggacg
     7741 ctcaaagcgc gcgtcgaagg ccccttccgg ataggccgcg ggccggtcga aggagaaggc
     7801 gaagccctcg gagattatcc gctccaggag gcccatgcgg gcgactccga tgccggagag
     7861 gatctccgcg cgcgtgcgct cgaaggtgta gcgcgtcctc gcgacggagc attccttgaa
     7921 gccctcaatc tcctcctcgg ccgcgcccgc cgcccagcct tcctcgtccc ggagaacggc
     7981 ggggggaggg aggtcgcgca gggcccggcg gatgacgatc gccgggcaga ggaggaagag
     8041 gcccgcgacg cggggtccga gggccctggc cgccccgcgg gcgaggtagc cgccgtagga
     8101 ctggcccgcg aggaggaagt cccctccgcc cgtcagttcg cctatcgcgt ccagcgtggc
     8161 cgcgagcatc gcgtcggagc tcgcttcctc gaggtcggcc gactcgccca tgcaggggag
     8221 gtcgaggtag atccgcctgt agctccgcga gctccgtccg ccctccggcc tccccgctcc
     8281 ggccgagggg ggcagggccg cccggccctc cgcgtcgaag aggggctcga aggccccgag
     8341 gctcatcctc cggtccagcg tgtagccgtg gagcgcgacg aaggggaagc ccgagccccg
     8401 ctcctcgtag gcgaggccgc cctcggtcgt cctcatgcga gtccctccct gagcgcggcg
     8461 gcgaaatcgg agaccgcccc gtcggcgccg ccgagcgccg cgttgaggac gaagaaggaa
     8521 tggatcat
//
LOCUS       DAOOSJ010000106         8475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5280_length_8475_cov_5.311045, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000106 DAOOSJ010000000
VERSION     DAOOSJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_8475_cov_5.311045"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="PLG14_06595"
     CDS             complement(<1..1682)
                     /locus_tag="PLG14_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOX48843.1"
                     /translation="MRSSEPGRGAFPSALRILPAAALAAAVLLAASLLASCAGAPAPA
                     PAAPAPEPEKPSLSALIGRKDGAAIREFFGSRELIDAADAEGLYPLHRAVLEDAPEIA
                     ELLLGLGAKPDPLDPKGRTPLRLALDAGRWAVARVLAERGASLFAKDAAGTSVAQAAI
                     AKGGEAFRAVFTAKTANARSEDGGSALHLAADALAEDAVAGLLDLGADPALRDKAGRS
                     PLDLALLHPDRIESARIAERLCLRGPAPSFPGYAWFAQAARASNYGSIRYENGNSPLH
                     EAVSAKAKGFVEFLLSKKAPPNARNGAGSAPLHEAVRSGWLEGAELLLKGGADPNVRD
                     GFDNTPLHIALPEAGRAEGVALLLRYGADPSLKDRNGNTPLHVAVQVGYPLALVETLL
                     GSGSPPNAANAAGDTPLHLALRSGRLEYAPALLARQADIFLANGKGETPLSLALGQVA
                     SQTGAGLAAILTPANVNGRDNLGNGPLAVATSLAAPPEAIALIVSEGGDPNARNNAGD
                     APLHLAVRRSLRPQGEALLAAKADVFAANARGETPLVLALTAPRGPEDWLFDS"
     gene            1849..>2781
                     /locus_tag="PLG14_06600"
     CDS             1849..>2781
                     /locus_tag="PLG14_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOX48844.1"
                     /translation="MKIGSFLPRSRLWDERLSALAARFPGHEFLLDPEASLAGAEGLD
                     AIVANRLPEEVYLRAAALKAVFVPFAGLNHLPAALLAGRGVRAFNAHGNAEEVAERAL
                     ALTLDFYGRVSEYHEDLKREVWHGFWVGHGSEDNWSSIFRRKAAVFGVGSIGQALARL
                     LKAFDCEVTGYRRRADSGLPPHFDRIETDFARAVSGSELLFVALPLTPETKGLFSREI
                     LASAKGKFLVNVGRGEVVDEEGLYLALKDGILKGAAIDTWYTYPQGGADRGAPSRYPI
                     HRLPNVILSPHVAGSAHEAAAANADQTVENIAEWL"
     assembly_gap    2782..2881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3014..4057)
                     /locus_tag="PLG14_06605"
     CDS             complement(3014..4057)
                     /locus_tag="PLG14_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48845.1"
                     /translation="MDSDRDSGKPGLVARADEARRLARSEPEAAERQARAVLAEAREA
                     GDSAALLRAHYTLGAIAVMDARYEEAVGRVAEGSAELAALGVGASPELLNVQGTAYRY
                     LGMYGLATERLLQAERLVRESDKPELVHPVLNNFALALSEIGEKEKAYGLFVEAEAAA
                     RAAGNDYVVLLTRSNIGSALTNLGRLDEALALLEATVESSRAAGLDSVLADALDNLGQ
                     ARTASGSLREAETDFRSALAIERRLGQAAVASLTREHLAENLLLQGRFAEAEAEARCA
                     YEAACRADHASLRSSSVLARAKALEALGRFREAAEAYRERVELDAADARAEGRAAYRE
                     LSARLAREAEARA"
     gene            complement(4103..5122)
                     /locus_tag="PLG14_06610"
     CDS             complement(4103..5122)
                     /locus_tag="PLG14_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48846.1"
                     /translation="MRDSLADPSLAAQAREALKLSDKDAPRAEALAREILAAALKAGD
                     RKAVLLSRYALGWIAVDAARFEEAAALAAEGLAELAAAGEPASPKLLNLEGSAYRYLG
                     MRGLAVERLQAAAALVRASGRAEDQYPVLNNLALVLLEVGESGRALELHFEAEAAAAA
                     AGASAALLGIRSNRGRILIELGRTEEAVPTLEETIAGAREPGLEPILADALAELGRAK
                     TLLGKLKEGEADLRAAREIERRLSRTAKASQAREVLAENLLAQGRLAEAEAESRAACE
                     AARVSGFEPLLSASVLLRARALEALGRYREAAEAYRERIELDEARNKAEGRAALRGGG
                     RGEGF"
     gene            complement(5295..7130)
                     /locus_tag="PLG14_06615"
     CDS             complement(5295..7130)
                     /locus_tag="PLG14_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX48847.1"
                     /translation="MKILFIQLPVQDPSWESSRANVPLAAGYLAAYAERQGLLKRGEW
                     SLLSREVSDYGSDTAVAEAIAREEPDLACFSLYLWNLDRSVHIAARVAALCPRTRLVA
                     GGPEVVSGMPVFAPPGTPGASPFHVLVEGEGELPFAALLGDLRRGKPLARRYAADKPL
                     DLLEVPNPYLAGTLPLEEDRPVHLETMRGCPARCGYCYYGKNYPEIRRFPRADAELVV
                     ELAGRAGAKEIYLMDPSFQATEDLPERLMGLARANKAGMSLHAELRLESVSEELGRLY
                     AAAGLLSAEVGLQSTNPKALEAVGRGWDRKAFERGAEIMAKNRVVVKTGVILGLPFDG
                     YEETIATFDFLGMQGLGQDAELYPLSLLPGTPVREKADEWGMTRLERPPYWVTSTDWI
                     SQDDLMDAIGAFEDGFDVEWAMPPAPHFREEAGGFRAFVDARRPESLDWMRLNPGKLA
                     NSLTLLVDADDPESVSRLSRAARDLRRDNPFALYQIVLRSDTRVPSEKLCARLCEAFA
                     NPEHYYDLSRSFSLDPQPSYQARMFFATKNPSLAYRAMEEAEDLETCLVVGGKGGFNA
                     DRLAEQLPFVIFDRDALPFDRLYELLMIYAEFRHMLVEAPEELWK"
     gene            complement(7134..8072)
                     /locus_tag="PLG14_06620"
     CDS             complement(7134..8072)
                     /locus_tag="PLG14_06620"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOX48848.1"
                     /translation="MRRRRREDPAFPRLGPEESFPFPDPRSADQSGIVCSGGNLSPGL
                     LLSAYSQGIFPWFSDNEPLLWWSPDPRFVLFPGRLHVSDTMRKLMRRFQAGKLPPGSA
                     AAAEALCGPAEEAATADSTMAALGGAGAAAPAAAAPSPGGAPPALPGLELSLDRDFPA
                     VIRACSGARRPGQRGTWITEDMIEAYIELHELGYAHSAEARLGGVLVGGLYGVSLGSA
                     FFGESMFSLVPDASKAAFIPLVLRLREEGFTLVDSQVYTDHLAGLGAEEIPREEYLRL
                     LAAALAAPTRRGDWSSLFPGFPASAEYRRLVPEVRP"
     gene            complement(8069..>8475)
                     /locus_tag="PLG14_06625"
     CDS             complement(8069..>8475)
                     /locus_tag="PLG14_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX48849.1"
                     /translation="SDYTEFPGGPVTAFMCFGLPRSVLSGAAFPSSHVAVSLMMCSFV
                     AKTERRLLPLYGLLTLLIALATVYIRAHWAADVAGGVLAALVLVPLFDRLRAPAQALA
                     DRASALAGLGAARSPGARRVAAPRAEAEAGQR"
BASE COUNT         1081 a         2843 c         3325 g         1126 t
ORIGIN      
        1 gagtcgaaga gccagtcctc ggggccgcgg ggcgcggtga gggccaggac gaggggcgtc
       61 tcgcccctcg cgttggccgc gaacacgtcc gccttggcgg cgaggagggc ctcgccctgg
      121 gggcgcaggg agcggcgcac ggcgaggtgg aggggcgcgt cgcctgcgtt gttgcgggcg
      181 ttcgggtcgc cgccctcgga gacgatgagg gcgatggcct cgggcggggc ggccagggag
      241 gtcgcgacgg cgaggggtcc gttccccagg ttgtcacggc cgttcacgtt ggccggcgtg
      301 aggatggcgg cgaggccggc cccggtctgc gaggcgacct ggcccagggc cagggagagg
      361 ggcgtctcgc ccttgccgtt ggcgaggaag atgtcggcct ggcgggcaag gagggcgggg
      421 gcgtactcga gccttccgct ccgcagggcc aggtggaggg gcgtgtcgcc cgcggcgttc
      481 gccgcgttgg gcggcgagcc cgagcccagg agggtctcca ccagggcgag gggatagccg
      541 acctggacgg ccacgtggag gggcgtgttg ccgttccggt ccttgaggga gggatcggcc
      601 ccgtacctga ggaggagggc gacgccctcc gccctgccgg cctcgggcag ggcgatgtgg
      661 aggggcgtgt tgtcgaagcc gtcgcgtacg ttggggtcgg ccccgccctt gaggaggagc
      721 tcggcgccct cgagccagcc cgagcggacc gcctcgtgga ggggggcgct gcccgcgccg
      781 ttgcgcgcgt tgggcggcgc cttcttcgag aggaggaact cgacgaagcc cttggccttc
      841 gcggacacgg cctcgtggag gggggagttg ccgttctcgt agcggatgga gccgtagttg
      901 gaggcccgcg ccgcctgcgc gaaccaggcg tagccgggga aggagggggc ggggccgcgg
      961 aggcagagcc gctcggctat cctcgcgctc tcgatccggt cggggtggag gagggccagg
     1021 tcgagggggc tgcggccggc cttgtcgcgc agggccgggt cggcccccag gtccaggagg
     1081 ccggcgaccg cgtcctccgc cagggcgtcg gccgcgaggt ggagggccga gccgccgtcc
     1141 tcgctccgcg cgttggcggt cttcgccgtg aagacggccc ggaaggcctc gccgcccttc
     1201 gcgatcgcgg cctgggcgac gctcgtgccc gcggcgtcct tcgcgaagag ggaggctccc
     1261 ctttccgcga ggacgcgggc gacggcccag cggcccgcgt ccagggccag gcggagcggc
     1321 gtcctcccct tggggtcgag ggggtcgggc ttcgccccca ggccgaggag gagctcggcg
     1381 atctccggcg cgtcctccag gacggcgcgg tggaggggat agaggccctc ggcgtcggcc
     1441 gcgtcgatga gctcgcggct gccgaagaat tcgcggatgg ccgcgccgtc cttcctgccg
     1501 atcagggcgg agaggctcgg cttctcgggc tcgggggccg gggcggccgg cgccggcgcg
     1561 ggggcgcccg cgcaggaggc caggagggac gcggccagga ggacggcggc cgcgagggcc
     1621 gcggcgggga ggatgcgcag ggccgagggg aaggctcccc gcccgggttc gctggatcgc
     1681 attgaggctc cttcgagcgt atgtcgcttc ttctttttcg gaatgttgat cgctataatc
     1741 tatcacgaag cgaggcccgc cgccaaaggc cgggctcgcg gaagcctaaa gccgcgcgga
     1801 gcgccccgtt ccggagccgc aggccaagaa ccgcaaggag agcccagcat gaagatcgga
     1861 tccttcctcc cccgctcccg cctctgggac gagcgccttt ccgccctcgc cgcccgcttt
     1921 cccgggcacg agttcctcct cgatcccgag gcctccctcg cgggggcgga aggcctggac
     1981 gcgatcgtag cgaaccgcct gcccgaggag gtctacctcc gcgcggccgc cctaaaggcc
     2041 gtcttcgtgc ccttcgccgg cctgaaccac ctcccggccg ccctcctggc cgggcggggc
     2101 gtgcgcgcct tcaacgccca cgggaacgcg gaggaggtcg ccgagcgggc cctcgccctg
     2161 actctcgact tctacggccg cgtctcggag taccacgagg acctgaagcg cgaggtctgg
     2221 cacggcttct gggtcggcca cggctcggag gacaactgga gctcgatctt caggcgcaag
     2281 gcggccgtct tcggcgtcgg ctcgataggc caggccctgg cccgcctcct caaggccttc
     2341 gactgcgagg tgacgggcta ccgccgccgc gccgactcgg gcctcccgcc ccatttcgac
     2401 cggatcgaga ccgacttcgc ccgggccgtc tcgggctcgg agctcctctt cgtcgccctt
     2461 cccctgacgc cggagacgaa gggcctcttc agccgcgaga tcctggcctc cgcgaagggc
     2521 aagttcctcg tcaacgtggg caggggcgag gtcgtcgacg aggaaggcct ctacctcgcg
     2581 ctcaaggacg ggatcctcaa gggcgcggcc atcgacacct ggtacaccta cccccagggc
     2641 ggcgcggacc ggggcgctcc ctcgcgctat ccgatccacc gcctgccgaa cgtcatcctc
     2701 tcgccccacg tcgccggctc ggcccacgag gcggccgccg ccaacgccga ccagacggtg
     2761 gagaacatcg cggaatggct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 ncgaggcggc cgccgccaac gccgaccaga cggtggagaa catcgcggaa tggcttgcct
     2941 cggggaagtg ccgccgcgag gtcgacctcg gggcgatgta ctagccgcgg gcctcccgcc
     3001 ggccgcgcgg cggctaggcg cgggcctcgg cctcccgcgc gagccgcgcg gagagttcgc
     3061 ggtaggctgc ccggccctcg gcgcgggcgt cggcggcgtc gagctcgacg cgctcgcggt
     3121 aggcctcggc cgcctcgcgg aagcgcccca gggcctccag ggccttcgcg cgcgcgagga
     3181 cggaggagga gcggagcgag gcgtggtcgg ccctgcaggc cgcctcgtag gcgcagcggg
     3241 cttcggcctc ggcctccgcg aagcggccct ggaggaggag gttctccgcc aggtgctccc
     3301 tggtcaggga ggcgaccgcg gcctggccca ggcggcgctc gatggccagg gccgagcgga
     3361 agtccgtctc cgcctcgcgg agcgagcccg aggccgtgcg ggcctggccc aggttgtcga
     3421 gggcgtcggc caggacggag tccagtcccg cggccctcga ggactccacg gtcgcctcga
     3481 ggagggcgag ggcctcgtcc agccgtccca ggttcgtgag ggcggagccg atgttcgacc
     3541 gcgtgaggag gaccacgtag tcgttgcccg cggcgcgggc ggcggcctcg gcctcgacga
     3601 agagtccgta ggccttctcc ttctcgccga tctcggagag ggccagcgcg aagttgttga
     3661 ggacggggtg gacgagctcg ggcttgtcgc tttcccgcac gagccgctcc gcctggagga
     3721 ggcgctccgt cgcgaggccg tacatgccga ggtagcggta ggccgtgccc tggacgttga
     3781 ggagctcggg gctcgctccg acgcccaggg cggcgagctc ggccgagccc tccgccacgc
     3841 ggccgacggc ctcctcgtag cgcgcgtcca tcaccgcgat ggctccgagg gtgtagtgcg
     3901 cccggaggag ggccgccgag tctcccgcct cgcgggcctc ggccaggacg gcccgcgcct
     3961 ggcgttcggc cgcctcgggc tcgctcctcg cgagcctcct ggcctcgtcg gcgcgggcga
     4021 cgaggcccgg ctttccgctg tccctgtcgc tgtccattgg aggattatat cacggatccg
     4081 gctccgcggc ggagccggcc ttctagaagc cctcgccgcg gcccccgccg cggagcgcgg
     4141 cccggccctc ggccttgttc cgcgcctcgt cgagctcgat ccgctcgcgg taggcctccg
     4201 ccgcctcgcg gtagcgtccc agggcctcca gggcgcgggc gcgcaggagg acggaggctg
     4261 agaggagggg ctcgaagccc gagaccctgg cggcctcgca ggccgcccgc gactcggcct
     4321 cggcctccgc gaggcggccc tgcgcgagga ggttttccgc gaggacttcc ctcgcctgcg
     4381 aggccttggc cgtcctcgag agcctgcgct cgatctcgcg ggcggcgcgg agatccgcct
     4441 cgccctcctt gagcttcccc aggagggtct tggcccttcc gagctcggcc agggcgtcgg
     4501 cgaggatggg ctcgagtccc ggctcccggg cgcccgcgat ggtctcctcc agggtcggga
     4561 cggcctcctc ggtccggccg agctcgatga ggatccgtcc ccggttggag cggatgccca
     4621 ggagggcggc cgaggccccc gccgcggcgg ccgccgcctc ggcctcgaag tggagctcga
     4681 gggcgcgccc ggactcgccg acctcgagga ggacgagggc caggttgttc aggacggggt
     4741 actggtcctc ggccctgccc gaggcgcgga cgagggccgc ggccgcctgg aggcgctcca
     4801 ccgcgaggcc gcgcatgccc aggtagcggt aggccgagcc ttccaggttg aggagcttgg
     4861 ggctcgcagg ctcgccggcc gcggcgagct cggcgaggcc ctccgcggcc agggcggccg
     4921 cctcctcgaa gcgcgcggcg tcgacggcta tccagcccaa ggcgtagcgg gagaggagga
     4981 cggctttgcg gtcgccggcc ttgagggccg cggcgaggat ctccctcgcg agcgcctcgg
     5041 cccgcggggc gtccttgtcg ctgagcttga gcgcttcccg cgcttgggcc gctaggctgg
     5101 ggtcggctag gctgtcgcgc atttccgtac tacgtatagc atcaagtccg ggcttcgcat
     5161 agcgggcttc gcatagcccg ccccgggctt aggctccctt gcccggcgcc tctacggagc
     5221 ggggcgtccc cgccgccggg ccactctacg gggccccgcg gccccgccgc ggagcggctc
     5281 cgccgcccga acccctactt ccagagctct tcgggcgcct ccacgagcat gtggcggaac
     5341 tccgcgtaga tcatgagaag ctcgtagagc cggtcgaagg gcagggcgtc gcggtcgaag
     5401 atcacgaagg gcagttgctc cgccaggcgg tcggcgttga agccgccctt gcccccgacg
     5461 accaggcagg tctccaggtc ctcggcttcc tccatcgcgc ggtaggcgag ggaagggttc
     5521 ttggtcgcga agaacatgcg ggcctggtag gagggctggg ggtcgaggga gaaggagcgc
     5581 gagaggtcgt agtagtgctc ggggttcgcg aaggcctcgc agaggcgggc gcagagcttc
     5641 tccgagggga cgcgggtgtc ggagcgcagg acgatctggt agagggcgaa ggggttgtcg
     5701 cggcgcaggt ccctcgcggc gcgggagagg cgggagacgg actcggggtc gtcggcgtcc
     5761 acgaggaggg tgagggagtt cgcgagcttc ccgggattga ggcgcatcca gtcgaggctc
     5821 tcgggcctgc gcgcgtccac gaaggcgcgg aagccgcccg cctcctccct gaagtggggg
     5881 gccgggggca tggcccactc gacgtcgaag ccgtcctcga aggcgcctat cgcgtccatg
     5941 aggtcgtcct ggctgatcca gtccgtgctc gtgacccagt acggcggccg ctccagccgc
     6001 gtcatgcccc actcgtccgc cttctcgcgc acgggcgtgc ccgggaggag ggagaggggg
     6061 tagagctcgg cgtcctggcc caggccctgc atgcccagga agtcgaaggt ggcgatggtc
     6121 tcctcgtagc cgtcgaaggg gaggcccagg atgacgcccg tcttcacgac gacgcggttc
     6181 ttggccatga tctctgcgcc gcgctcgaag gccttccggt cccagccgcg gcccacggcc
     6241 tccagggcct tggggttcgt gctctggagg cccacctccg ccgagaggag gcccgcggcg
     6301 gcgtagaggc ggccgagttc ctcggagacg gactcgaggc ggagctcggc gtggaggctc
     6361 atccccgcct tgttggcccg cgcgaggccc atgaggcgct cgggcaggtc ctccgtcgcc
     6421 tggaaggagg gatccatgag gtagatctcc ttcgccccgg cgcggcccgc gagctcgacg
     6481 acgagctccg cgtcggcgcg ggggaagcgg cggatctccg ggtagttctt gccgtagtag
     6541 cagtagccgc agcgggccgg gcagccccgc atcgtctcca ggtggacggg cctgtcctcc
     6601 tccaggggaa gggtgcccgc gaggtagggg ttcgggacct cgaggaggtc gaggggcttg
     6661 tcggcggcgt agcggcgggc gaggggcttt cccctgcgga ggtccccgag gagggccgcg
     6721 aagggcagct ccccctcgcc ctcgacaagg acgtggaagg gcgaggctcc gggcgtcccc
     6781 gggggcgcga agacgggcat gccggagacg acctcgggcc cgcccgcgac gaggcgggtc
     6841 cggggacaga gggcggcgac ccgggcggct atgtggacgc tgcggtccag gttccagagg
     6901 tagagggaga agcaggcgag gtcgggctcc tcccgggcta tcgcctccgc gacggccgtg
     6961 tccgagccgt agtcggagac ctcgcgggaa aggaggctcc actcgccgcg cttgaggagg
     7021 ccctgccgct cggcgtaggc cgcgaggtag cccgccgcga gggggacgtt ggcgcgggag
     7081 ctctcccagg agggatcttg gacgggcagc tggatgaata ggatcttcat gtcttacggc
     7141 cgtacttccg ggacgaggcg acggtactcg gccgaggcgg gaaagccggg gaagagggag
     7201 ctccagtcgc cccgccgcgt gggcgcggcg agtgcggccg cgaggaggcg gaggtactct
     7261 tcccggggga tctcctcggc gcctaggccg gcgaggtggt cagtatacac ttgactgtcg
     7321 acgagggtga agccctcctc cctgaggcgg aggacgaggg ggatgaaggc cgccttggag
     7381 gcgtcgggca cgaggctgaa catggactcg ccgaagaagg ccgagcccag ggagacgccg
     7441 tagaggccgc cgaccaggac gccgcccagg cgggcctccg cggagtgggc gtagcccagc
     7501 tcatggagct cgatgtaggc ctcgatcatg tcctccgtga tccaggtgcc gcgctggccc
     7561 ggcctgcggg cgcccgagca ggccctgatg acggcgggga agtcgcggtc cagggagagc
     7621 tcgaggccgg ggagggccgg gggcgcgccg ccgggcgagg gcgcggccgc ggctggcgcg
     7681 gccgccccgg cgccgcctag cgcggccatg gtcgagtctg ccgtcgccgc ctcttccgcg
     7741 gggccgcaga gggcctcggc cgcggccgcg ctcccgggcg gcagcttccc ggcctggaag
     7801 cgccgcatga gcttccgcat cgtgtcggag acgtggagcc gcccgggaaa gaggacgaag
     7861 cgggggtcgg ggctccacca gaggagtggc tcgttgtcgg agaaccaggg gaagatgccc
     7921 tgggaatagg ccgagaggag gaggcctggc gagaggtttc cgcccgagca gacgatgccc
     7981 gactggtccg cgcttctcgg gtccgggaag gggaaggact cctcgggccc gagccgcggg
     8041 aaggcggggt cctcccggcg gcgtcgcctc accgctgccc ggcctccgcc tccgccctcg
     8101 gggcggcgac gcgccgggcg cccgggctcc tggccgcgcc gagtcccgcg agggcgctcg
     8161 cgcggtcggc cagggcctgg gcgggggcgc gcagccggtc gaagaggggg acgaggacga
     8221 gggccgcgag gacgccgccc gcgacgtcgg ccgcccagtg ggcgcggatg tagaccgtcg
     8281 cgagggcgat gaggagggtg aggaggccgt agaggggaag gaggcggcgc tccgtcttcg
     8341 cgacgaagga gcacatcatg agggagaccg cgacgtggct ggaggggaag gccgcgcccg
     8401 agaggaccga ccgcggaagg ccgaagcaca tgaaggcggt gacggggccg ccggggaact
     8461 ccgtgtagtc ggagg
//
LOCUS       DAOOSJ010000107         8420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5313_length_8420_cov_6.243276, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000107 DAOOSJ010000000
VERSION     DAOOSJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5313_length_8420_cov_6.243276"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLG14_06630"
     CDS             <1..1072
                     /locus_tag="PLG14_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX48850.1"
                     /translation="RFVYREQAMAFRAKLLEAKEASVSVAMPESVYKNLGRRYSRRAP
                     PLDFAVSFSFMGDRYDLAFPTTRQFDPVSEPEPSESFDPADIRALVRDFNEKAGEYAS
                     ERGIHMFKDRKPETAEERLVVRTGLCFYLPSAAGGLPLVDPYVSPRIVTRTAFADWLR
                     EQGVREGLVDEELLRYERNKKSSGVLSELLVPVVFQEYVIGCVSLQNKLPGKAPFDLG
                     ILDTFHQFAKILAYSLKLNGYFRDAPKKASGFPADVIDVSAGGLLFANPSRELAGSLL
                     PGSRLELQVKAGGRSVKAKAVLKRSYRDSELVYYGVEYDEIAPEDFRFLFEALYGRAF
                     TDGDAQSVEGLGIKVLPDLKFD"
     gene            complement(1322..1870)
                     /locus_tag="PLG14_06635"
     CDS             complement(1322..1870)
                     /locus_tag="PLG14_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48851.1"
                     /translation="MANENLKNGILEIRVDENSPLFDKTGMLHKEFPSDFRQIRYFTL
                     LIAQSAPPDIKEINLLEQQISEIIKNAVKHGNKCDVEKKVRVWYSFSPEHAHLVVEDE
                     GEGFKDLEKWNEFNRKRQSCLDSGNFEALADYVSFRTIKSDDNDGGNALFAALEYWDG
                     GFVFNQKRNCIAMLKRFPKRRL"
     gene            complement(1876..2214)
                     /locus_tag="PLG14_06640"
     CDS             complement(1876..2214)
                     /locus_tag="PLG14_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX48852.1"
                     /translation="MELKIRKSGESYIIDVNGEMDLYNSYRLKELVMRMLEKKVERFI
                     INLENVDYIDSSGIGALIYICSTIKKMNLKLVITNIHGSVKKVIELTKLMGYFPITQS
                     IEEALERMEA"
     gene            complement(2237..4180)
                     /locus_tag="PLG14_06645"
     CDS             complement(2237..4180)
                     /locus_tag="PLG14_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX48853.1"
                     /translation="MISATIAEALSGPLLVKLVLAPILGAAFLYLRRKETEGVMTGYA
                     FLAAFLGLRDVAFALFPFPDLYRASDLIFFGILCWVQAASFGARLGRWLPLAFELLAA
                     ALLVLKSALGFAPGVPSELFGLAALVPVLVNFAVSSLGRAEADTPGRSLVVRVRFPLL
                     AGSVFYLAASALLGPSSFAFQAFVIPLFYSLGLYFAFMFVDILHRELVGAVGYYEESI
                     DSLYELLLSSGSAMKAEFSIQEVLDNMAGVIAEKAGADGSAILLADEFEEAVSVRAVH
                     GRFPPPWKLPDSLPRAEDRVAAFVRHARFRLGEGLLGEVAQSGKHLFVPDAAVDPRIP
                     RNGEEDWLRLTSLIAAPLVVRDRVIGAVALSKSGPEPFTERDFDRAKLLANFGSIAVA
                     NSFSFLEAAERSDIDREASIAEGIQKTIAPRKIPELAGVSFGAYTSPARGVCSDYYDV
                     IQTRADRAVLVVGDVAGKGVNAGIVMAMVRSIIHLITNSAKDTATLLSWVNRGISGKV
                     DTDHFATLSLVSVDASTGEAEFSNAAHQPLLIYRKDADAIETVDIKSIPIGVERATEY
                     AAKRLRFRPGDVLVMYTDGVVEAMNEQGRQFGRKNLGNAILRCRELSAKEIAEAIRGD
                     VMEFSGRTKQHDDQTVLVMKAKL"
     gene            complement(4304..4645)
                     /locus_tag="PLG14_06650"
     CDS             complement(4304..4645)
                     /locus_tag="PLG14_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX48854.1"
                     /translation="MSGERRQDARDRSFARILARVEGRAGYVADVSENGFRAIFAGDF
                     SPALDSPLRVEISFEELGLGIFPLPALARWSRAVEGGAEAGFELAPEAREGPEAGLFE
                     RLRSYYDKSLR"
     gene            complement(4642..5298)
                     /locus_tag="PLG14_06655"
     CDS             complement(4642..5298)
                     /locus_tag="PLG14_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX48855.1"
                     /translation="MAPYIAYRSQRGETLQLAFSPRAEFRDIVDSFAEIAYPRLAVSR
                     ELVSFALLELVSNSVRAHREKAVAEEVRVGFSAGGGELSVTILDAGRGFDPTLLPYDL
                     EAPPDSIDLLGDSFAEYRERHGGSRFGMGLYIAKRTFPRFRLSFVDREGRPCPWFSGS
                     VKGTRIDLAAPLAPEAPRPPLVRLASESASEEELEVLFEPAGVLEPAMAPLGAKGAAR
                     "
     gene            5355..8330
                     /locus_tag="PLG14_06660"
     CDS             5355..8330
                     /locus_tag="PLG14_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48856.1"
                     /translation="MIGIFVKIPFHAQQKRADPALAAELSRAVAEAASVFGARILPSE
                     ESFFLAFDEGTGCPRLRAAEAARSLGARLARLAPRLHGYALILGEGPLGSEELERELR
                     RSWYLVRKDGLFIEEGSAGGFRGYVETDEGEGVLGVLAFPYTRPLYPETYAPPSPDAG
                     ALDRFVDEAGRHGIGESEADLLLAVGPGASPGAHVEAALGLLYGQKAASFLRLRASVG
                     ESAPYGPFLEAVATKPDQAGLALLSAPERQALEELAPVGDFLASSPYRQGYTPTIRTR
                     TRLYAAARLRLYARLQRAAGLPALVVLEGLDRFPPESLDLVSELFGEGLAEEGVVVLG
                     TAERPPLGLPRARLRILAAPPPSPAAIAAAATAGAEGLGERSLAPRLAALAEGDPFRL
                     GLALAAAGRAARRSPSGEESAPRLPGGRLGTEALVALALAAFPSEYAEYLLALGLAEE
                     VLDQPRLGEFLRKSGFLPGVEPLLRETLAGLGLVGAAGRPRLSRAEALAGAEAVSGEG
                     AARIKAGFAELLLSLHLEGRILPGAALFRRVAASGAPLDSGGRQGARRVERSRFFLDC
                     VAADDLYGPSERPGTQALDIPLAGLGAFLEAYALADGEEAERRLADLDAKALGPGSEP
                     LAAAIAALARASDEYARGSAQAAAARAKAALIALHDLGAARSEARAHRLLGLAALAQA
                     QVQEGAYYLANAFELAAAQKDPFECFHAAFAEAGAQLVLGDLRRAEQRGRAAAEWAAK
                     AFRADWEAAADFLSGRVAFELGRYEAAAESFGRVRAAARVYGEPEAAFRAEVWAGRAA
                     AYSGEYGRARELLARRKDDAEAAWFLGECAYFEGDEAEAARSGREALDLIPERPYASA
                     DALLWGSGFDSFEARSLGAGRGRSYLEDQVLAFSRFAEGLARRDPSLVAAIAALTREE
                     RLAALHPAAHLYHFYCCRILEATGAASPDPGTVLSKAFKALQTRSVRIEEASLKTEFL
                     EKNRWNAALMAAARAKKLL"
BASE COUNT         1213 a         2990 c         2974 g         1243 t
ORIGIN      
        1 ccgcttcgtc taccgcgagc aggccatggc cttccgcgcc aagctcctgg aggccaagga
       61 ggcgagcgtc tccgtcgcga tgcccgaatc cgtctacaag aacctggggc gccggtacag
      121 ccgacgggcg ccgcccctcg atttcgccgt ctccttctcc ttcatgggcg accgctacga
      181 cctcgccttc cccacgacca ggcagttcga ccccgtctcc gagccggagc cctcggagag
      241 cttcgacccc gccgacatcc gcgccctcgt gcgcgacttc aacgagaagg ccggcgagta
      301 cgcgagcgag cggggcatcc acatgttcaa ggacaggaag cccgagacgg cggaggagcg
      361 gctggtcgtg cggaccggcc tctgcttcta cctgccctcg gccgcgggcg gcctccccct
      421 cgtcgacccc tacgtgagcc cgaggatcgt gacgcggacg gccttcgccg actggctccg
      481 cgagcagggc gtgcgcgagg gcctcgtgga cgaggagctc ctgcgctacg agcgcaacaa
      541 gaagtcctcc ggcgtcctgt cggagctcct cgtgcccgtg gtcttccagg agtacgtcat
      601 cggctgcgtg agcctgcaga acaagctgcc cggcaaggcg cccttcgatc tcggcatcct
      661 ggacaccttc caccagttcg cgaagatcct ggcctactcc ctcaagctca acggctactt
      721 ccgcgacgct cccaagaagg cctccggctt cccggccgac gtcatcgacg tgagcgcggg
      781 cggcctcctc ttcgcgaatc cctcccgcga gctcgccggc tccctcctcc ccggctcgag
      841 gctggagctc caggtcaagg ccggcgggcg ctccgtcaag gcgaaggccg tcctcaagcg
      901 gagctaccgc gactcggagc tcgtctacta cggcgtcgag tacgacgaga tcgccccgga
      961 ggacttccgc ttcctcttcg aggccctcta cggccgggcc ttcacggacg gggacgcgca
     1021 gagcgtcgag gggctcggga tcaaggtgct ccccgacctg aaattcgact aagcggggag
     1081 ggaaaccttt cgtttcgagc gaaaggtttc cctccccgcg cccctccctt ccaaaggcgc
     1141 gaccggggct ccgccccggc ccccgcaccg cctgcaaaac cgtcgttccg gccccagagg
     1201 cggattgccg aaccgcgcgc aaaaaaagac cggagccgtt cacggctccg gtcggcgctc
     1261 gacgcggaaa acggcgccat cggcccgcgg ccgaggggcg gcgaggctaa aggcggcggg
     1321 actagaggcg gcgcttgggg aagcgcttga gcatcgcgat gcagttgcgc ttctggttga
     1381 agacgaagcc gccgtcccag tactccaggg cggcgaagag ggcgttgccg ccgtcgttgt
     1441 cgtcgctctt tatcgtgcgg aaggagacgt agtccgcgag ggcctcgaag ttgccggagt
     1501 ccaggcagga ctggcgcttg cggttgaact cgttccactt ctccaggtcc ttgaagccct
     1561 cgccctcgtc ctcgacgacg aggtgggcgt gctcggggct gaaggagtac cagaccctga
     1621 ccttcttctc gacgtcgcac ttgttgccgt gcttcaccgc gttcttgatg atctcgctga
     1681 tctgctgctc gaggaggttg atctccttga tgtcgggcgg cgcgctctgg gcgatgagga
     1741 gggtgaagta gcggatctgg cggaagtccg aggggaattc cttgtggagc atgcccgtct
     1801 tgtcgaagag gggggagttc tcgtcgacgc gtatctccag gatgccgttc ttgaggtttt
     1861 cgttagccat gggactcagg cctccatgcg ctcgagggct tcctcgatgc tctgcgtgat
     1921 cgggaagtag cccatgagct tcgtgagctc gatgaccttc ttgaccgagc cgtggatgtt
     1981 cgtgatgacg agcttcaggt tcatcttctt gatcgtcgag cagatgtaga tgagcgcgcc
     2041 gatgccgctc gagtctatgt agtcgacgtt ctcgaggttg atgatgaagc gctcgacctt
     2101 cttctcgagc atcctcatca cgagctcctt gagcctatac gagttgtaga ggtccatctc
     2161 gccgttcacg tcgatgatgt agctctcgcc gctcttgcgg atcttcaatt ccatgggatt
     2221 tgtggctcct cccctctcaa agcttcgcct tcattacgag cacggtctgg tcgtcgtgct
     2281 gcttggtccg tccggagaat tccatgacat cgccgcggat ggcctcggct atctccttgg
     2341 ccgagagctc gcggcagcgg agtatcgcgt tgcccaggtt cttgcggccg aactgccgcc
     2401 cctgctcgtt catcgcctcg accacgccgt cggtgtacat cacgaggacg tcgcccgggc
     2461 ggaagcgcag gcgcttggcc gcgtactccg tcgccctctc gacgccgatc gggatgctct
     2521 tgatgtccac ggtctcgatc gcgtcggcgt ccttccggta gatgaggagg ggctggtggg
     2581 ccgcgttgga gaactccgcc tcgcccgtcg aggcgtcgac ggagacgagg gagagggtcg
     2641 cgaagtggtc ggtgtcgacc ttgcccgaga tgccccggtt cacccaggac aggagggtgg
     2701 ccgtgtcctt ggccgagttc gtgatgaggt ggatgatcga gcggaccatc gccatcacga
     2761 tgcccgcgtt gacgcccttg ccggcgacgt cgcccacgac gaggacggcg cggtcggccc
     2821 gggtctggat gacgtcgtag tagtcagagc agacgccccg cgcggggctc gtgtaggcgc
     2881 cgaaggagac gcccgcgagc tcggggatct tcctgggcgc gatcgtcttc tggatgccct
     2941 ccgcgatgga ggcctcgcgg tcgatgtcgc tgcgctcggc cgcctccagg aaggagaagg
     3001 agttcgcgac ggcgatggag ccgaagttgg cgaggagctt ggcccggtcg aagtcccgct
     3061 cggtgaaggg ctcgggcccg gacttggaga gggccacggc gccgatgacg cggtcccgca
     3121 cgaccagggg ggcggcgatg agggaggtga ggcggagcca gtcctcctcg ccgttccgcg
     3181 ggatccgggg atcgacggcc gcgtcgggca cgaagaggtg cttgccgctc tgggccacct
     3241 cgccgaggag gccctcgccc aggcggaagc gggcgtggcg gacgaaggcg gcgacgcggt
     3301 cctccgcgcg gggcaggctg tcggggagct tccagggcgg cgggaagcgg ccgtggacgg
     3361 cgcggacgga gaccgcctcc tcgaactcgt cggcgaggag gatggcgctc ccgtcggccc
     3421 ccgccttctc ggcgatgacc ccggccatgt tgtccaggac ctcctggatg gagaactccg
     3481 ccttcatcgc cgagcccgag gagaggagga gctcgtagag gctgtcgatc gactcctcgt
     3541 agtacccgac ggcgccgacg agctcgcggt ggaggatgtc gacgaacatg aaggcgaagt
     3601 agaggcccag ggagtagaag agggggatca cgaaggcctg gaaggcgaag gaggaggggc
     3661 cgaggagggc ggaggccgcc aggtagaaga cggagcccgc gaggaggggg aagcggaccc
     3721 gcacgacgag gctgcggccg ggggtgtcgg cctcggcgcg gcccagggag gagaccgcga
     3781 agttgaccag gacggggacc agggccgcga ggccgaagag ctcggagggg acgccgggcg
     3841 cgaagcccag ggcggacttg aggacgagga gggccgcggc gagaagctcg aaggccaggg
     3901 gcagccagcg cccgaggcgg gcgccgaagg aggcggcctg cacccagcag agtatcccga
     3961 agaagatgag gtcggaggcg cggtagaggt cggggaaggg gaagagggcg aaggcgacgt
     4021 ccctgaggcc gaggaaggcg gcgaggaagg cgtagcccgt catcacgccc tcggtctcct
     4081 tcctcctgag atagaggaag gccgcgccga ggattggcgc gaggacgagc ttgacgagca
     4141 gcggcccgct cagcgcttct gcgattgtcg ccgaaatcat gaacaatccc ctcgcggacg
     4201 gtcggccgga gcgccgccaa ggcgcggccg ccccggattc cggagcggat tcacccgacc
     4261 ggttgcgagt ctaccaccct ggaaaagccg gcgtcaagtt tattcaccgg agcgacttgt
     4321 cgtagtagct cctgaggcgc tcgaaaagcc ccgcctccgg cccttcccgg gcctccggcg
     4381 cgagctcgaa gccggcctcg gcgccgccct ccacggccct gctccagcgg gccagggccg
     4441 gaagggggaa gatccccagg ccgagctcct cgaaggagat ctccacgcgc aaggggctgt
     4501 ccagggccgg ggagaagtcc cccgcgaaga tggccctgaa gccgttctcc gagacatcgg
     4561 ccacgtagcc ggcccggccc tcgacccgcg cgaggatgcg ggcgaagctc ctgtcccggg
     4621 cgtcctggcg ccgctcgccg ctcaccgcgc ggctcccttg gcccccaggg gcgccatggc
     4681 gggctccagc acgccggcgg gctcgaagag cacctcgagt tcctcctccg aggcgctctc
     4741 ggaggcgaga cggaccaggg gcggcctcgg ggcctccggc gcgagggggg cggccaagtc
     4801 gatcctcgtc cccttcacgg agcccgagaa ccagggacag ggcctcccct cgcggtcgac
     4861 gaaggagagg cggaagcgcg gaaaggtgcg cttggctatg tagagtccca tgccgaagcg
     4921 gcttcccccg tggcgctccc ggtactccgc gaaggaatcc ccgaggaggt ctatggagtc
     4981 gggcggcgct tccaggtcgt aggggaggag ggtcgggtcg aagcccctcc ccgcgtccag
     5041 gatcgtgacg gagagctccc cgccgccggc cgaaaagccc acgcggacct cctcggccac
     5101 ggccttctcg cggtgggcgc gcaccgagtt ggagacgagc tcgaggaggg cgaaggagac
     5161 gagctcccgg ctgaccgcga gccgggggta ggcgatctcg gcgaaagagt cgacgatgtc
     5221 gcggaactcc gcgcgcgggg agaacgcgag ctgtaatgtc tcgcctcgtt gcgatcgata
     5281 agctatatag ggcgccatac tttttcgcgc taatcctacg cctgggggcg taaacccgca
     5341 agggcggaac ggccatgatc ggcatcttcg tcaaaattcc cttccatgcc cagcagaagc
     5401 gcgccgatcc ggccctcgcc gcggagctct cccgggccgt ggcggaggcg gcctccgtct
     5461 tcggggccag gatcctcccc tccgaggagt ccttcttcct cgccttcgac gaggggacgg
     5521 gctgcccccg gctcagggcg gccgaggccg cgaggagcct gggcgcccgc ctcgcccgcc
     5581 tcgctccgcg cctccacggc tacgccctca tcctgggtga ggggcccctg gggagcgagg
     5641 agctcgagcg ggagctcagg cggagctggt acctcgtgcg caaggacggc ctcttcatcg
     5701 aggagggctc ggcgggcggc ttcaggggct acgtcgagac ggacgagggc gaaggagtct
     5761 tgggcgtctt agccttcccc tacacgaggc cgctctatcc cgagacctac gccccgccga
     5821 gccccgacgc cggggccctg gaccgtttcg tcgacgaggc cggcaggcac ggcatcggcg
     5881 agtcggaggc cgacctcctc ctcgccgtcg gccccggagc ctcgccggga gcccacgtgg
     5941 aggcggccct gggcctcctc tacggccaga aggccgcttc cttcctcagg ctccgcgcct
     6001 ccgtcgggga atcggccccc tacggcccct tcctggaggc cgtggcgacg aaacccgacc
     6061 aggccggcct ggccctcctc tccgcccccg agcgccaggc cctcgaggag ctcgcccccg
     6121 tcggcgactt cctcgcctcc tccccctacc gccagggcta cacgccgacg atccgcacga
     6181 ggacgcgcct ctacgcggcc gcccgcctcc gcctctacgc ccgcctgcag cgcgcggccg
     6241 gcctccccgc cctcgtcgtc ctggagggcc tggaccgctt cccgccggag agcctcgacc
     6301 tcgtctccga gctcttcggc gagggcctcg cggaggaggg cgtcgtcgtc ctggggacgg
     6361 cggagcgccc tcccctcggc ctgccccgcg cgcggctgcg catcctggcc gccccgccgc
     6421 cctcccccgc cgccatagcc gcggccgcga cggccggggc cgaaggcctg ggcgagcgga
     6481 gcctcgcgcc ccgcctcgcg gccctggccg agggcgatcc cttcaggctg ggcctggccc
     6541 tcgccgcggc cggccgggcc gcccgcaggt cgccctcggg ggaggagtcc gcgccccggc
     6601 tgcccggcgg gcggctgggc acggaagccc tcgtcgccct ggccctggcc gccttcccct
     6661 ccgagtacgc ggagtacctc ctggccctcg gcctcgcgga ggaggtcctg gaccagccgc
     6721 ggctcgggga attcctccgc aagtcgggct tcctccccgg cgtcgagccc ctcctccggg
     6781 agaccctcgc cggcctgggc ctcgtgggcg cggccggcag gccccgcctg tccagggccg
     6841 aggccctcgc gggcgcggag gccgtctcgg gcgagggcgc ggcgcggatc aaggcgggct
     6901 tcgccgagct cctcctctcc ctgcacctgg aggggcggat ccttcccggc gcggccctct
     6961 tcaggcgcgt cgccgcctcg ggcgcgcccc tggactcggg cgggcgccag ggggcgcgcc
     7021 gcgtcgagag gagccgcttc ttcctggact gcgtcgcggc cgacgacctc tacggcccct
     7081 cggagcggcc ggggacccaa gccctcgaca taccgcttgc cggcctgggc gccttcctcg
     7141 aggcctacgc cctggccgac ggggaggagg ccgagcgccg cctcgccgac ctggacgcga
     7201 aggccctggg ccctggctcc gagcccctgg ccgccgcgat agccgccctg gccagggcct
     7261 ccgacgagta tgcccggggc tcggcccagg cggccgccgc ccgcgccaag gccgccctca
     7321 tcgccctcca cgacctgggc gccgcgagat ccgaggcgag ggcccaccgc ctcctcggcc
     7381 tcgcggccct ggcccaggcc caggtccagg agggcgccta ctacctcgcg aacgccttcg
     7441 agctggccgc ggcccagaag gaccccttcg agtgcttcca cgccgccttc gcggaagccg
     7501 gcgcccagct cgtccttggg gacctcaggc gcgccgagca gcgcggccgg gccgcggccg
     7561 agtgggccgc caaggccttc cgcgcggact gggaggcggc ggcggacttc ctctcgggcc
     7621 gcgtcgcctt cgagctcggc cgctacgagg cggctgcgga gagcttcggc cgcgtgcgcg
     7681 ccgccgcccg cgtctacggc gagccggagg ccgccttccg cgccgaggtc tgggccggca
     7741 gggcggcggc ctactcgggc gagtacggca gggcgcggga gctcctcgcg cggcggaagg
     7801 acgacgccga agccgcctgg ttcctgggcg agtgcgccta tttcgagggc gacgaggccg
     7861 aggccgcgcg ctcgggaagg gaagccctgg accttatccc ggagcggccc tacgcctccg
     7921 cagacgccct cctctggggc tcgggcttcg actccttcga ggcgcggtcc ctgggcgcgg
     7981 gccgcggccg gagctacctg gaggaccagg tcctcgcctt ctcgcgcttc gcggagggcc
     8041 tcgcgcggcg cgatccctcc ctcgtcgccg ccatcgcggc cctcacccgc gaggagcgcc
     8101 tcgcggccct ccatcccgcg gcccacctct accatttcta ctgctgccgc atcctggagg
     8161 cgaccggcgc ggcctctccc gacccgggca ccgtcctctc caaggccttc aaggccctcc
     8221 agacgaggag cgtccgcatc gaagaggcga gcctcaagac cgagttcctg gagaagaacc
     8281 gctggaacgc cgccctcatg gccgccgccc gcgccaagaa gctcctctag cccggaccgc
     8341 gcgggctcgc ggcgtgaagg cgacgaaccc cgccgccgtt ttatgagacg aggcaggacg
     8401 gcgggtgccc ggccgcagac
//
LOCUS       DAOOSJ010000108         8385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5337_length_8385_cov_5.784514, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000108 DAOOSJ010000000
VERSION     DAOOSJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5337_length_8385_cov_5.784514"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLG14_06665"
     CDS             complement(<1..340)
                     /locus_tag="PLG14_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48857.1"
                     /translation="MRTDKRSWAAYLAFPLFIAALLVVILVFRGELVALFRDREATRA
                     WIQARGGWGKLAFVGLQALQVFLFVIPGEVVQVAGGYVFGLWEGTLLSLLGITLGSLV
                     KELERLGIGRP"
     gene            496..993
                     /locus_tag="PLG14_06670"
     CDS             496..993
                     /locus_tag="PLG14_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOX48858.1"
                     /translation="MASPTTAYAVDQRIVYPSQGVGRILEIREKSFKDSKILYYIIYL
                     EFSDMTVMVPTEKADDLGIRAIVPKAEAEKALAFISEEYAPIPTDWKLRYQMNLDLLK
                     KGSVLDIASVVRSLYHRSKIKELPILERKLYDSALNILQDEISFSLGKSKDEIAELIR
                     ARLEG"
     gene            995..>1299
                     /locus_tag="PLG14_06675"
     CDS             995..>1299
                     /locus_tag="PLG14_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOX48859.1"
                     /translation="MPRSSCRLAVLVTAAGSSERFGGGKKELLAVEGRSILDRALSSF
                     IFLPDLAFLAVTAPPGRAEELRSALSVQTLAALGKRLIFVDGGPSRRDSVRLGLEALA
                     "
     assembly_gap    1300..1399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1400..2342
                     /gene="ispF"
                     /locus_tag="PLG14_06680"
     CDS             <1400..2342
                     /gene="ispF"
                     /locus_tag="PLG14_06680"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOX48860.1"
                     /translation="GPGGDEETIVLVHDGARPWASPALALSVLEAAKRQGAAVPVLPL
                     VDTPKRLSPEGLIEAHPERSSLGGAQTPQGFRLFALLEAHRRALAEGLECTDDAELWA
                     RYLGPVAWVPGERENRKVTYREDLAAAPGEAPLERGKRRRGRISEEGARAVLPFRVGE
                     GWDLHRLVQGRRLLVGGVEIPSEFGELAHSDGDVLLHAVIDALLGAAALGDIGSHFPP
                     SDERWRGADSRELARRAAFLVREAGYEPGNLDCTVVLERPRLGPHREAILASLAETLG
                     MPPSSISFKAKTSEGVDAAGEGRAVEARAVVLLSRRD"
     gene            complement(2501..3376)
                     /locus_tag="PLG14_06685"
     CDS             complement(2501..3376)
                     /locus_tag="PLG14_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48861.1"
                     /translation="MSQAAAALLALAGNFVLSLGMVLQKRHVGWLGRKGGLGRESRRD
                     RRGWTAGFVLMNLAPVFNYFALLGLAPNVVAAMIGSSVAFTTLLAALVLKERIGARRL
                     CWTGGLFAALALAGLRGEAAGASFSPAALAACAALPPAAGLLLAAARERRRRRGADGQ
                     GGGGLPASGLPRRGLSKRGLAAAFAAAAGAAGGSMVLSMRAVQLSGTGSPLSWFGTPY
                     LYGYAVAGIAAFALIQLAYKDGEMSSVSPVFYGMQVLWPALSSYAVFAAPFDLVEALS
                     FGAIALAILFISRSR"
     gene            complement(3373..4230)
                     /locus_tag="PLG14_06690"
     CDS             complement(3373..4230)
                     /locus_tag="PLG14_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX48862.1"
                     /translation="MHGYVAALIALSGNFLLSLGMVLQKQNVGWFGHKGEKERKYRRA
                     RRGWILGFVLMNLQPVFNYFALFGLPPNVVAAMIGANVAFTSVLAALMLGERLTRRRL
                     AGAVVIFAAVGAISLTGERAQAGIVEVRAGFYYAFGAFPFALAAVLAAFRRRIRSRAF
                     PTMVAAVAGALGGYMVITMKALQLEAAGGPLTWLATPYFYVYFASGAASFSILQLAFH
                     RGEVTTVSPADYGMQVLWSALAAQFVFGMPIDLVHQAAILAILASIVAIASSGRRGLH
                     AQSEGGQGR"
     gene            complement(4250..6241)
                     /locus_tag="PLG14_06695"
     CDS             complement(4250..6241)
                     /locus_tag="PLG14_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOX48863.1"
                     /translation="MISIEAARDGFNLMFGGRRLLAHSRRSPCVEIGWSELSFAQRRG
                     AFRLARRKSSWRPLKSFKVVENGPDFTAIDFEGLLRMAIRLEDGALRLSFARFDAGVE
                     LFRLRFQAWPGERVYGGGARLSRLDLKGLALPLWAEDRQGGGEGALSRAVGALRGYGP
                     RPLSSRCPIPAFVSSRGYWLVLDSSARSELDFRRRGRGVIESWAVPREIVVGTAPDAP
                     ALLASLGSAVGKQPRLPSWAYDGAILGVSGGLESAKAAAGAALAAGAKLSSLVLEDRR
                     VGGLVFRPSRPAGGAGTSAAAGADPAELAQAAAGFRDRGIRLLGRADPFVAREGPRFE
                     EARDAGFLVKDSKGEPYFLPVEGGQAAMVDLTDPGAAAWLKASLKRDFLGAGLSGWLA
                     DCGEQLPADALLASGEDPRKAHNRWPLLWARLCREAIDEAGRQGDALFLMRSGWLGST
                     RYLPSLWIEPSRARDPRGGGLQGALLAALSLGLSGVGCCHVEAAPPSEKGRRGLEAAA
                     RILEQAAFSPLLRLRSGGQGRASDPDFLPRFARMSEAYAALKPYHLATAEEYQAGGLP
                     AIRHPYVHYEGEAGLHLRAGQYLYGRDLLVAPAPGPGRESTELELPDDEWVHLWSSRR
                     FRGGSVTVESPLGFPAVFYRAASGFAPLFDALRRTTRRA"
     gene            complement(6334..7230)
                     /locus_tag="PLG14_06700"
     CDS             complement(6334..7230)
                     /locus_tag="PLG14_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOX48864.1"
                     /translation="MNVAVLASPDDVASLVPILDVLRQVGAPAYGLKIRDSWESLPRG
                     EIFRRVDKASHFLVLCSRGSAAKSWFSFAAGFGVGRGSRTALFRLEPSWDPPPYLATL
                     PILDDLEELASYFRLERAEWLVQETRRNARSALLEMGISFHADSLAQCVRDGDLKAVE
                     LFLRAGHHPDARDRHGVPLLCLAARGRHLSVANLLVEAGASLDLQSEDRGYSALMDAV
                     HSGAAEIVELLLSRGAALDLRSKDGQTALVVAVGKGDARMAKRLLDFGADPDAADKLG
                     LSARRYAALFKDPAMLAAFEGK"
     gene            complement(7348..8343)
                     /locus_tag="PLG14_06705"
     CDS             complement(7348..8343)
                     /locus_tag="PLG14_06705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOX48865.1"
                     /translation="MRKREAYSPSAPRLPAAPRGPAGQGPSGPRRVRLDGPRRARLAG
                     PRRARLAGALILCLLAAAPAASLEPPRAPAPRAGWPLGEAGVRIAGSEAAPAGFPSSA
                     PFRDRAVILCWHSFLGDPSLPTDFSLAELKAQLDALAALGYRFPSFAEAVSGRLEGPL
                     NVVVTLDDGHRTVPAAVERVFSELGIKPSLFVYPSVIGTQAFAMDDAALGRLSSEGCL
                     VGAHGYHHLFVTADLARRDRAAFDREIHKAKEKTEAITRLPVVAYAYPFGALSQVTKE
                     ELVRAGYAYGLAVAPGFVYADEAFDDLLELPRLVVTREGWKDILALLSRNARAFR"
BASE COUNT         1258 a         2902 c         3143 g          982 t
ORIGIN      
        1 acgggcggcc gatgccaagc cgctccagct ccttgacgag ggagcccagc gtgatgccga
       61 gaagggagag gagcgtgccc tcccagaggc cgaagacgta gccgcccgcg acctggacga
      121 cctcgcccgg gatcacgaaa aggaagacct gcagggcctg gaggccgacg aaggcgagct
      181 tgccccagcc tcccctggcc tggatccagg ccctcgtcgc ctcgcggtcg cggaacagcg
      241 cgaccagctc gcccctgaag acgagaatga cgacgaggag ggccgcgatg aagaggggga
      301 aggctaggta ggcggcccag gatctcttgt cggtcctcat gccgccactc tagtacggcg
      361 cgcgggaggg ccgcaaggcc gagggacgcg gccgaccgag gggcagggcg cggcgcctgc
      421 ggcgcttcgc gcggcaccgt acggcgcttt gcccggtact ttaaatttac ccaattttgg
      481 agtatagttg gcgccatggc ttcccccacc accgcctacg ccgtcgacca gcgcatcgtc
      541 tacccgagcc agggtgtcgg caggatcctc gagatccgcg agaaatcctt caaggactcg
      601 aagatcctct actacatcat ctacctcgag ttctcggaca tgacggtgat ggtgccgacg
      661 gagaaggccg acgacctcgg catcagggcc atcgtgccca aggccgaggc ggagaaggcc
      721 ctggccttca tctcggagga gtacgccccg atccccacgg actggaagct gcgctaccag
      781 atgaacctgg acctcctcaa gaagggctcc gtcctcgaca tcgcctccgt ggtgcgctcc
      841 ctctaccacc gctccaagat caaggagctg ccgatcctgg agcgcaagct ctacgactcg
      901 gccctcaaca tcctccagga cgagatctcc ttctccctcg gcaagtcgaa ggacgagata
      961 gccgaactca tccgcgcccg cctcgagggc tagcgtgccc cgctcctcct gccgcctcgc
     1021 cgtcctcgtg acggccgcgg gctcgagcga gcgcttcggc ggcgggaaga aggaactcct
     1081 cgccgtcgag gggcgctcca tcctggaccg cgccctctcc tccttcatct tcctacccga
     1141 cctcgccttc ctcgccgtca cggcccctcc gggccgggcc gaggagttgc gctccgccct
     1201 ctccgtccag accctcgccg ccctgggcaa gcgcctcatc ttcgtcgacg gaggcccgag
     1261 ccgccgcgac tcggtgcgcc taggcctcga ggccctcgcn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnc ggccctggcg gcgacgagga gaccatcgtc ctcgtccacg
     1441 acggcgcgag gccctgggcc agccccgccc tcgccctgag cgtcctcgag gcggcgaagc
     1501 ggcagggcgc cgccgtgccc gtcctccccc tcgtcgacac gcccaagcgc ctctcgccgg
     1561 agggcctcat cgaggcccac cccgagcgct cctccctggg cggagcccag acgccccagg
     1621 gcttccgcct ctttgccctc cttgaggcgc accgccgcgc cctcgcggag ggcctcgagt
     1681 gcacggacga cgccgagctc tgggcgcgct acctcggccc cgtcgcctgg gtgccgggag
     1741 agcgggagaa ccgcaaggtc acctaccgcg aggacctcgc ggccgctccc ggcgaggcgc
     1801 cgctcgagcg cggcaagcgc cgccgcgggc ggatctccga ggagggggcc agggccgtcc
     1861 tccccttccg cgtcggcgag ggctgggacc tccaccgcct ggtacagggc cggcgcctcc
     1921 tcgtgggcgg cgtggagatt ccctcggaat tcggcgagct cgcgcactcc gacggcgacg
     1981 tcctcctcca cgcggtcatc gacgccctcc tcggggcggc ggccctgggg gacatcggct
     2041 cccacttccc gccctccgac gagcgctggc gcggggccga ttcgcgggag ctcgcgcgcc
     2101 gcgcggcctt cctggtccgc gaggcgggct acgagccggg caacctggac tgcacggtgg
     2161 tcctggagcg gcccaggctc ggaccgcacc gcgaggccat cctcgcctcc ctcgcggaga
     2221 ccctgggcat gcccccctcc tccatctcct tcaaggccaa gacgagcgag ggcgtggacg
     2281 ccgcgggcga gggccgggcc gtggaggccc gcgccgtcgt cctcctgtcc aggcgggact
     2341 gagggcgggc acgacccgcg gatgccgcga gcctcgctgc ccccgcggcg agccttgcct
     2401 cgcgcggcgg gccgcctgac gccgcgacga tccattgctc gcgcggccgg cggccccgcc
     2461 tctttcggcc ggacgccggc ggccggcgat tgccgccgga ttacctggac cgcgagatga
     2521 agaggatcgc cagcgctatc gccccgaagg acagcgcctc gacaaggtcg aaaggcgcgg
     2581 cgaagaccgc gtaggacgag agggccggcc acagcacctg catcccgtag aagaccggag
     2641 agaccgagga catctccccg tccttgtagg cgagctggat gagggcgaag gccgcgatgc
     2701 ccgcgaccgc gtagccgtag aggtagggag taccgaacca gctcagcggc gagcccgtgc
     2761 ccgagagctg gacggcgcgc atggagagca ccatcgagcc gccggccgcg ccggccgccg
     2821 ccgcgaaggc cgccgccagg ccgcgcttcg agagcccgcg ccgtggaagg ccgctggctg
     2881 gaaggccgcc gccgccctgg ccgtccgcgc cgcgccgccg ccgccgctcc ctcgcggcgg
     2941 cgaggaggag gccggccgcc gggggcaggg ccgcgcaggc cgcgagggcc gccggggaga
     3001 agctcgcgcc cgcggcctcg ccgcgcagcc ccgcgagggc cagggcggcg aagagcccgc
     3061 ccgtccagca gaggcgcctc gccccgatcc gctccttgag gacgagggcc gcgaggaggg
     3121 tcgtgaaggc gacgctcgag ccgatcatcg cggccacgac attgggcgcg aggccgagga
     3181 gggcgaagta gttgaacacg ggggccaggt tcatgaggac gaagcccgcc gtccagcccc
     3241 gccggtcgcg cctcgactcc cgccccaggc ctcccttcct tcccagccag ccgacatggc
     3301 gcttctgcag gaccatgccc agggagagga cgaagttgcc cgcgagggcg aggagggcgg
     3361 cggcggcctg gctcatcgcc cctgcccgcc ctcggactgc gcatggagtc cgcgccgccc
     3421 cgagctcgcg atggcgacga tggaggcgag gatcgccagg atggccgcct ggtggacaag
     3481 gtctatgggc atcccgaaga cgaactgcgc cgcgagggcg gaccagagga cctgcatccc
     3541 gtagtcggcc ggcgagaccg tcgtcacctc gccgcggtgg aaggcgagct ggaggatcga
     3601 gaagctcgcg gcgcccgagg cgaaatagac gtagaagtag ggcgtcgcga gccatgtgag
     3661 ggggccgccc gcggcctcga gctggagggc cttcatcgtg atcaccatgt agccgcccag
     3721 ggcgcccgcg acagctgcca ccatcgtcgg gaaggcccgg ctcctgatcc ggcgccggaa
     3781 ggcggccagg acggcggcca gggcgaaggg gaaggcgccg aaggcgtagt agaagcccgc
     3841 gcgcacctcg acgatgcccg cctgggccct ctcgcccgtg aggctgatgg cccccacggc
     3901 ggcgaagatg acgacggcgc ccgcgagcct gcggcgcgtc aggcgctctc ccagcatcag
     3961 ggcggccagc acggaggtga aggcgacgtt ggcgcctatc atcgcggcga cgacgttggg
     4021 cggcaggccg aagagggcga agtagttgaa gacgggctgg aggttcatga ggacgaagcc
     4081 gaggatccag cctcgcctgg cccggcggta cttccgctcc ttctcgccct tgtgcccgaa
     4141 ccagccgacg ttctgcttct ggaggaccat gccgagggag aggaggaagt taccggagag
     4201 cgcgatgagg gccgcgacgt agccgtgcat ggacgcaagt atagcgcggc taggccctgc
     4261 gcgtcgtcct ccgcagggcg tcgaagaggg gcgcgaagcc ggaggcggcc cggtagaaga
     4321 cggcggggaa gcccaagggg ctctccaccg tcacggagcc gcccctgaag cgcctcgagc
     4381 tccagaggtg gacccattcg tcgtcgggga gctcgagctc ggtcgactcg cggcccgggc
     4441 ccggagcggg cgcgaccagg aggtcgcggc cgtagaggta ctggccggcc ctcaggtgca
     4501 ggcccgcctc gccctcgtag tggacgtagg ggtggcggat cgccggaagg ccccccgcct
     4561 ggtactcctc cgcggtcgcg aggtggtagg gcttcagggc cgcgtaggcc tccgacatgc
     4621 gcgcgaagcg cggcaggaag tccgggtcgg aggccctgcc ctgccccccg ctccggaggc
     4681 gcaggagggg cgagaaggcg gcctgctcca gaatccgcgc cgcggcctcg agcccgcgcc
     4741 ggcccttctc cgaaggaggg gccgcctcga cgtggcagca gccgacgccc gagaggccca
     4801 gggagagggc ggcgaggagg gctccctgta ggccgccccc gcgcgggtcc cgggcccgcg
     4861 agggctcgat ccagagcgag ggcaggtagc gcgtcgagcc gagccagccc gagcgcatga
     4921 ggaagagcgc gtcgccctgg cggccggcct cgtcgatcgc ctcgcggcag agccgcgccc
     4981 agaggagggg ccagcggttg tgcgccttgc ggggatcctc gccggaggcg aggagggcgt
     5041 cggcgggcag ctgctcgccg cagtcggcga gccagcccga gaggccggcg cccaggaagt
     5101 cgcgcttcaa agaggccttg agccaggccg ccgcgcccgg atcggtgagg tcgaccatcg
     5161 cggcctggcc gccctccacg gggaggaagt agggctcgcc cttcgagtcc ttgacgagga
     5221 agcccgcgtc gcgggcctcc tcgaagcgcg gcccctcgcg cgcgacgaag gggtcggccc
     5281 ggcctaggag gcggatgccc cggtcgcgga agccggccgc ggcctgggcg agctcggcgg
     5341 gatcggcgcc cgccgccgcg gaggttcccg cgccacccgc cgggcgggag gggcggaaga
     5401 cgagcccgcc cacgcgcctg tcctccagca cgagggagga gagcttcgcg cccgcggcca
     5461 gggcggcgcc ggccgcggct ttggcggact cgaggccccc tgagacgccc aggatggcgc
     5521 cgtcgtaggc ccaggaggga aggcgcggct gcttgcccac ggccgagccc agggaggcga
     5581 ggagggcagg ggcgtcgggg gccgtcccga cgacgatctc gcgggggacg gcccaggact
     5641 cgatgacgcc gcggccgcgg cgccggaagt cgagctcgga gcgggcgctc gagtccagga
     5701 cgagccagta gccgcgcgag gagacgaagg ccgggatcgg gcaacgcgag gaaagggggc
     5761 gggggccgta gccgcgcagg gccccgacgg cccgggagag ggcgccttcg ccgccgccct
     5821 ggcggtcctc ggcccagagc ggaagggcca ggcccttgag gtcgaggcgg gagagcctgg
     5881 ctcctccgcc gtagacccgt tcgccgggcc aggcctggaa gcggagccgg aagagctcca
     5941 cgccggcgtc gaagcgcgcg aaggagaggc ggagcgcgcc gtcctccagg cggatggcca
     6001 tcctgaggag gccctcgaag tcgatggcgg tgaagtcggg gccgttctcc acgaccttga
     6061 aggacttgag ggggcgccag gagctcttcc gcctggccag cctgaaggcg ccgcggcgct
     6121 gggcgaagga gagctccgac cagcctatct ccacgcaggg ggagcggcgg ctgtgggcca
     6181 ggaggcgcct gccgccgaac atgaggttga aaccgtcccg cgcggcctcg atgctgatca
     6241 ccccgacagc ctccggtcag cggccttgtc cgctacaggg cccaccatac accggccccg
     6301 cggcgggcgc aacccgaatc ccgaaggatg acgttatttg ccctcgaagg ccgcgagcat
     6361 cgccgggtcc ttgaagagcg ccgcgtagcg cctggcggag aggccgagct tgtccgccgc
     6421 gtcggggtcg gcgccgaagt cgaggagccg cttggccatc ctggcgtcgc ccttgcccac
     6481 ggccacgacc agggccgtct gcccgtcctt gctccgcaag tccagggctg cgccgcggga
     6541 gaggaggagc tccacgatct ccgcggctcc cgagtggacc gcgtccatta gggcggagta
     6601 gccccggtcc tcgctctgga ggtccaggga ggcgccggcc tcgacgagga ggttggcgac
     6661 ggagaggtgc ctgcccctcg cggccaggca gaggaggggc acgccgtggc ggtcgcgggc
     6721 gtcggggtgg tggcccgcgc ggaggaagag ctcgacggcc ttgaggtcgc cgtcgcggac
     6781 gcactgcgcc agggagtcgg cgtggaagga gatgcccatc tccaggaggg ccgagcgggc
     6841 gttccgcctc gtctcctgga cgagccactc ggcccgctcc aggcggaagt aggaggcgag
     6901 ctcctccagg tcgtcgagga tgggcagggt cgcgaggtag ggaggggggt cccaggaggg
     6961 ctcgagccgg aacagcgccg tcctcgagcc tcggccgacc ccgaagccgg ccgcgaagga
     7021 gaaccagctc ttggcagcgc tcccgcggga gcagaggacg aggaagtggc tcgccttgtc
     7081 gacccggcgg aagatctcgc cccggggcag gctctcccag ctgtcgcgga tcttgaggcc
     7141 gtaggccggc gccccgacct gccggaggac gtcgaggatg gggacgaggg aggcgacgtc
     7201 gtcgggcgag gcgaggactg caacgttcaa gactttccct tcctccgggg cccgggggcg
     7261 agggtccgcc cgcgggattc cggcgcgcgg gatcctggcg cgcgggaaaa cccctattga
     7321 actagtcgga aacggcggcg gcccggctta gcgaaaagcg cgcgcgttcc gggaaaggag
     7381 ggcgaggatg tccttccagc cctcccgcgt cacgacgagc ctggggagct cgaggaggtc
     7441 gtcgaaggcc tcgtccgcgt agacgaagcc cggggccacc gcgaggccgt aggcgtagcc
     7501 cgcgcggacg agctcctcct tcgtgacctg ggagagggcg ccgaagggat aggcgtaggc
     7561 gacgacgggc aggcgggtga tcgcctcggt cttctccttg gccttgtgga tctcccggtc
     7621 gaaggcggcc ctgtcgcgcc tggcgaggtc ggccgtgacg aagaggtggt ggtagccgtg
     7681 ggcgccgacc aggcagcctt cggaggagag gcggcccagg gcggcgtcgt ccatggcgaa
     7741 ggcctgggtc ccgatgacgg agggatagac gaagaggctc ggcttgatgc ccagctcgga
     7801 gaagacccgc tccacggcgg ccggcaccgt cctgtgcccg tcgtccaggg tcacgacgac
     7861 gttcaggggg ccctccaggc ggcctgagac ggcctccgcg aagctgggga agcggtagcc
     7921 cagggcggcg agggcatcga gctgggcctt gagctccgcg agggagaaat ccgtcggcag
     7981 ggagggatcg ccgaggaagc tgtgccagca gaggatgacg gcgcggtccc ggaagggcgc
     8041 cgaggagggg aagcccgcgg gggcggcctc gcttccggct atcctgacgc ccgcttcgcc
     8101 taaaggccag ccggcccggg gagccggagc ccggggaggc tcgagggagg cggcgggcgc
     8161 ggcggcgagg aggcagagga tgagggcgcc ggccaggcgg gcgcggcgcg gaccggccag
     8221 gcgggcacgg cgcggaccgt ccaggcggac gcggcgcgga ccggacggcc cctggccggc
     8281 gggcccgcgg ggagcggcgg gaagccgcgg ggcggagggg gaataggctt ctctcttgcg
     8341 catcgacccc acccatgtat acgatccccg ccgccttttg caagg
//
LOCUS       DAOOSJ010000109         8215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5471_length_8215_cov_5.531127, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000109 DAOOSJ010000000
VERSION     DAOOSJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5471_length_8215_cov_5.531127"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLG14_06710"
     CDS             complement(<1..698)
                     /locus_tag="PLG14_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX48866.1"
                     /translation="MIDKKAWPSFIPRPDYLERIKPFIGKGLIKVLTGQRRVGKSYIL
                     YQLMDEIATRNPEVGITYINKELHEFRDIRDGDDLHRFVSGRLKADTPNALFIDEIQE
                     IKGFELALRSLLAEGRCDIYCTGSNADLLSGELATFLAGRHVEIRIHPLSFAEFLRFH
                     NLQDSRESLALYLRLGGMPYLSAIGLDEAVAFEYLDNVHASILLRDVVAREKIRNVDF
                     LETLLEYVADNVGNL"
     gene            complement(864..936)
                     /locus_tag="PLG14_06715"
     tRNA            complement(864..936)
                     /locus_tag="PLG14_06715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(901..903),aa:Met,seq:cat)
     gene            complement(1189..1887)
                     /locus_tag="PLG14_06720"
     CDS             complement(1189..1887)
                     /locus_tag="PLG14_06720"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOX48867.1"
                     /translation="MPTCPDCGVELAEDARACPLCGKPLGDARPHAHEKYIDPEDRER
                     LTERERRTIAWEVLSVSSAIAAVAVLAVNLIDSGALTWALYPLFCLAFAWTVFTGVFL
                     LYRKPMLAILFPALALHAFLLGLDLVDGRLTWALPLALPIALDAELSAAAAWLLSRVS
                     KRKGANLIALALLAVVTLCIGVEFTLDRFLSGRVALSWSAIVAFTLVPIACFLFYLHY
                     RVGKKTNLRKLFRL"
     gene            complement(1887..3239)
                     /locus_tag="PLG14_06725"
     CDS             complement(1887..3239)
                     /locus_tag="PLG14_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48868.1"
                     /translation="MAGKDSAERLNRRKARTGFRDESGSWYILDNAATIMPPTTDSVA
                     TGLFRVSADLDAPVNLPSLQVALERTARRFPYFSVELRRGLFWYYLEPHEKPVRVELD
                     PESPSQSFRMRRRGTCLFRVRVRGRRIACEFSHALTDGTGGVRFLKTLLVEYFRLRGV
                     EAGGEDPDVYRLDEKPEAEEYEDAYNRHFSGVYPHPEAGKPAFQLGARLLPRHQYRVI
                     SGVLPLAPALAKAKEFGVSLTELLAAAYLDALQSIWFSAPEKERRRQRPVAAVEIPVN
                     MRKFYPTKSNRNFSLFVLLTQDYRLGRRDFAEIVQRAHHQMRMETDAPSIARQISRNV
                     AGTRSLFVRLVPLFVKDFFARILFQAFGENLISGFISNLGPVSLPPGPAAHVERFDFV
                     PAPSKVTKTNASVLSWKGNLVVDFGSLGKSRELERLFFSRLASLGLPVRIECNLREES
                     "
     gene            complement(3288..3986)
                     /locus_tag="PLG14_06730"
     CDS             complement(3288..3986)
                     /locus_tag="PLG14_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOX48869.1"
                     /translation="MLATLINAAAIIVGSLIGILLKKGLSARYEATVFAATGIISLVI
                     GIQMALKASHVLAFALALIIGGLVGTLLDIEGGFLKLGERLKARFAAKDEGSTFAYGF
                     LDASVLFCAGAMAIVGSFKAGTEGDYAILLTKSVMDGFISVLFASAMGVGVAFSALSV
                     LVYQGALTLLSVLVKPYVTETMLAELTGLGGALVIMIGINLLGLKKLKTGDFLPALVV
                     TVLLVLAMPYVPFL"
     gene            4074..4400
                     /locus_tag="PLG14_06735"
     CDS             4074..4400
                     /locus_tag="PLG14_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48870.1"
                     /translation="MVEKLEALREAEAVLADEGAADRVFCANCIHCKLVPSPSGAEGS
                     YRLRVRCDAGKWRKKIGEEKVYKYFTVAKRSIPACDAYEDMGDAKEFIRDLRKALPTS
                     DELYNF"
     gene            4410..4817
                     /locus_tag="PLG14_06740"
     CDS             4410..4817
                     /locus_tag="PLG14_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48871.1"
                     /translation="MRKTATILAAALAAALSLALLSSCASAPKEIPEDLTSRELIQRA
                     QEASDGDNFAAAIAYYEAARERYGYDPAVLATAEYEVSFIYYKQGKYAEAEAGFLSLL
                     ARYDGPEGESLPNTFKILAEKLLPKVREAMGKK"
     gene            complement(5006..5287)
                     /locus_tag="PLG14_06745"
     CDS             complement(5006..5287)
                     /locus_tag="PLG14_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48872.1"
                     /translation="MDAKNGLVNFGLFILLFVFTFVFCLDALALPNTTYGVLALIGYV
                     VCVAASLFTGILSRRDGEALSVWYFCYAVVVSIVFVWYLTRCGTAFGWW"
     gene            complement(5386..7383)
                     /locus_tag="PLG14_06750"
     CDS             complement(5386..7383)
                     /locus_tag="PLG14_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX48873.1"
                     /translation="MAFVQFSDVSLAFGARDILKNATLYLAAGSRAALAGPNGAGKTT
                     LMRIVAGQLSPDSGERAVTKGARVSYLPQTGVVHGGGTVYEEAERAYDFAAAMLGRQE
                     EIGRLLEDSTSDGGQTARLLEEHAAIHEAVEGSGYFRRRERIREILAGLGFRDAEMDR
                     PVAEFSGGWQMRVALAKVLLENPDIMLLDEPTNYLDLEAREWLEAFLSKYPGGVLVVS
                     HDRYFLDATVREVYELWNGRLTRYAGTYSAYERRRAEELEAVFEAWERQQEEIQKLEE
                     FIRRFRYQATKASLVQSRVKQLEKIVPIEIPEGMKKIHFSFPPAPHSGKATVKCLGVG
                     KSYGAHRVLSGLDLEIERGMKVAFVGPNGAGKSTLMRLIAGTDRDYEGEIRFGSGVET
                     AYFAQDTADLMGGDATVEETVEALCPFDLLPKVRNLLGAFLFRGDDVEKPVRVLSGGE
                     RSRLALLTMLLKPANLLILDEPTNHLDLTSKDVLLEALKSFQGTVLFVSHDRGFIEEL
                     ADRVLELEPDESARWYVGNYDYYLQKKALLAEERQAEAAGSGGAGAASGGRGAQPSGA
                     RPEAAAQAAGAATALSYEEEKARKARLRKLAKREEEILARLESLGAQKAACEREMGLP
                     ANYSDGARMRRLQASVEELEAEAEALNAEWESVAEELSAVS"
     gene            complement(7386..>8215)
                     /locus_tag="PLG14_06755"
     CDS             complement(7386..>8215)
                     /locus_tag="PLG14_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48874.1"
                     /translation="SVSQVFDYDSLSEEGGALVFEGTPLRVRFEGERRLVADWSGRSA
                     QALQPEAEEPGSAGPAADSGAEAPKPGADGRAVFTILSKELRDAVRDEELRRQKLLDA
                     FLAFGEEWVYEPSGAEPPDPAAAQAPASSLVLSRSKRFSWTGVGALPSGYLPEDVAAA
                     LGEAPGLAFASPAETVAGAAPQTGAAGSAGAAPPGESAKAATGEAAFRLFLAPSLSGE
                     WSGAVSLRFDQGSGGWTDFLFRLRDGSLELEPAVGVSGLEVGSASSLPPLRFAPKRR"
BASE COUNT         1407 a         2644 c         2961 g         1203 t
ORIGIN      
        1 agattgccca cattgtcggc gacgtattca aggagggtct cgaggaagtc gacgttgcgg
       61 atcttctcgc gggcgacgac gtccctgagc aggatggacg cgtggacatt gtcgaggtac
      121 tcgaaggcga cagcttcgtc caggccgatc gcggagaggt agggcattcc cccgagcctc
      181 aggtaaagcg cgaggctctc ccttgaatcc tggagattgt ggaagcgcag gaactcggcg
      241 aagctcaagg gatggatccg gatctcgacg tggcgccccg caagaaaggt ggcgagttcg
      301 ccggagagga ggtcggcgtt gctgcccgtg cagtagatgt cgcatcgccc ttcggctagg
      361 agactccgaa gcgcgagttc gaagcccttg atctcctgga tctcgtcgat gaagagggcg
      421 tttggcgtat cggccttgag cctgccggaa acgaatcgat gcagatcatc gccatcgcgg
      481 atgtcccgaa attcgtggag ttccttgttg atataggtga tcccgacctc gggattccgc
      541 gtcgcgatct cgtccatgag ctgatacagg atgtagcttt tcccgacccg ccgctgtcct
      601 gtcaggacct tgataagtcc cttacctata aacggcttga tgcgctcaag gtaatcgggg
      661 cggggaatga aggatggcca tgccttctta tcaatcatgt tttataatct atagcagtat
      721 cttgttttta tcaatcttgt tttacaagac tcttgtcagc aaagaacaaa gaaaaagccc
      781 cgcgtcgccg cggagcttca tctttaggct tcatcgggaa ttaccccgat tacggttgaa
      841 cataggtgcc tgacggaaac ctatagcagg ggcaggactc gaacctgcga cctccgggtt
      901 atgagcccga cgagctgcca actgctctac cctgcgtcgt gttgagatga atctacatta
      961 aacgagaaga aaggtcaata gatgcaaagg acaatccccg gaaaaaggga aaagtccaga
     1021 ggcgattcta cgattccgcg cgagggcccg ggcgcgggtc ccggccttcc cttagcccgc
     1081 ggagccccgg cccgccgcga caaaccgccg cttggcgacc gcagggcccc tacccgcccg
     1141 caagcgccgc ggctcgcggc tgtccgaccg gccgcgcccc ctcccccgct agagcctgaa
     1201 gagcttccgc aagttggtct tcttcccgac ccggtaatgc aggtagaaga ggaaacaggc
     1261 tataggcacc agggtgaagg cgacgatagc cgaccagctc agggccacgc ggccggaaag
     1321 gaaacggtcc agggtgaact cgacgccgat gcagagggtg acgacggcga ggagggcgag
     1381 cgcgatgagg ttggcgccct tccgcttgga gacgcggctc aggagccagg ccgccgccgc
     1441 cgagagctcg gcgtccaggg cgatgggcag ggccaggggc agggcccagg tgaggcggcc
     1501 gtccaccagg tccaggccca ggaggaaggc gtgcagggcc agggcgggga agaggatcgc
     1561 gagcatgggc ttccggtaga ggaggaagac gccggtgaag accgtccacg cgaaggccag
     1621 gcagaagagg ggatagaggg cccaggtcag ggcgcccgag tcgatgaggt tgacggcgag
     1681 gacggcgacg gccgcgatcg ccgaggagac ggagaggacc tcccaggcga tggtgcggcg
     1741 ctccctctcg gtgagccgct ccctgtcctc ggggtcgatg tacttctcgt gggcgtgggg
     1801 ccgcgcgtcg ccgagcggct tgccgcagag cgggcaggcg cgggcgtcct cggcgagctc
     1861 gacgccgcag tccggacatg tgggcatcag ctttcctccc taaggttgca ttcgatccgc
     1921 acggggagcc ccaggctcgc gaggcgggaa aagaagagcc gctcgagctc cctcgacttg
     1981 cccaggctcc cgaagtcgac cacgaggttc cccttccagc tcaggaccga ggcgttcgtc
     2041 ttcgtcacct tgctgggcgc ggggacgaag tcgaagcgct cgacgtgggc ggccggcccc
     2101 gggggcaggc tcaccgggcc caggttcgag atgaagcccg agatgaggtt ctccccgaag
     2161 gcctggaaga gtatccgggc gaagaagtcc ttgacgaaga ggggcaccaa gcgcacgaag
     2221 aggctccttg tccccgccac gttccgcgag atctgccgcg cgatggaggg ggcgtccgtc
     2281 tccatccgca tctggtggtg ggccctctgc acgatctccg cgaagtcccg cctgccgagc
     2341 ctgtagtcct gggtgaggag gacgaagagg ctgaaattgc ggttggactt ggtcgggtag
     2401 aacttgcgca tgttgaccgg gatctccacc gcggcgacgg ggcgctggcg gcgccgctcc
     2461 ttctcgggcg cggagaacca gatcgactgc agggcgtcca ggtaggcggc cgcgaggagc
     2521 tccgtgaggg agacgccgaa ctccttggcc ttcgcgaggg cgggggccag gggcaggacg
     2581 cccgagatga cccggtactg gtgccgcgga aggaggcggg cgccgagctg gaaggcgggc
     2641 ttccccgcct ccggatgggg atagacgccc gagaagtgcc ggttgtaggc gtcctcgtac
     2701 tcctcggcct cgggcttctc gtccaggcgg tagacgtcgg gatcctcgcc tccggcctcc
     2761 acgccgcgga gccggaagta ctcgacgagg agggtcttga ggaagcggac gccgccggtg
     2821 ccgtcggtga gggcgtggga gaactcgcag gcgatccgcc ggccccggac gcgcacgcgg
     2881 aagagacagg tgccccggcg ccgcatgcgg aaggactggc tcggcgactc ggggtccagc
     2941 tccacccgga cgggcttctc gtggggctcg aggtagtacc agaagagccc gcgcctgagc
     3001 tccacggaga agtaggggaa gcgcctcgcc gtcctctcca gggcgacctg gagggagggg
     3061 aggttgaccg gcgcgtccag gtcggcggag acgcggaaga gccccgtggc caccgagtcg
     3121 gtggtcgggg gcatgatggt cgccgcgttg tccaggatgt accaggagcc cgactcgtcg
     3181 cggaagcccg tcctggcctt ccgcctgttg aggcgctcgg cgctgtcctt ccccgccatg
     3241 gcgaaagcct ttccccttcc ggagcgcttg cggtcttggg gcgagtccta caggaagggg
     3301 acgtagggca tcgcgagcac gaggagcacg gtgacgacga gcgcgggcag gaagtcgccc
     3361 gtcttgagct tcttgaggcc gaggaggttg atgccgatca tgatgacgag ggcgccgccc
     3421 aggccggtga gctccgcgag catcgtctcc gtgacgtagg gcttcaccag gaccgagagg
     3481 agggtgaggg cgccctggta gacgaggacc gagagggcgg agaaggcgac gccgacgccc
     3541 atcgcgctcg cgaagaggac ggagatgaag ccgtccatca cgctcttcgt gaggaggatc
     3601 gcgtagtcgc cctcggtgcc cgccttgaag gagccgacga tggccatagc gccggcgcag
     3661 aagaggacgg aggcgtccag gaagccgtag gcgaaggtcg agccctcgtc cttcgcggcg
     3721 aagcgggcct tgagccgctc gcctagcttc aggaagccgc cctcgatgtc gaggagggtc
     3781 ccgaccaggc cgccgatgat gagggccagc gcgaaggcca ggacgtggct ggccttgagg
     3841 gccatctgga tgccgatcac gagggagatg atgcccgtcg cggcgaagac cgtcgcctcg
     3901 tagcgggcgg agagcccctt cttgaggagg atcccgatga gggagcccac gatgatggcg
     3961 gcggcgttga tgagggtggc aagcatgggg aactccggac ggcggcccgg tcgcgccgag
     4021 ccttgaccgc tggaaataga gtatagcata atcggggagt aaagggggcc gggatggtcg
     4081 aaaagctcga ggcgcttcgc gaggccgaag ccgttcttgc cgacgaggga gccgcggacc
     4141 gcgtgttctg cgctaactgc atccactgca agctcgtccc ctccccctcg ggcgccgagg
     4201 gcagctaccg gctccgcgtc cgctgcgacg cgggcaagtg gcggaagaag atcggcgagg
     4261 agaaggtcta caagtacttc accgtcgcca agcggagcat ccccgcctgc gacgcctacg
     4321 aggacatggg cgacgcgaag gagttcatcc gcgacctccg caaggccctc cccacgagcg
     4381 acgaactcta caacttctaa ggaagcccca tgagaaagac cgcgacgatc ctcgcggcgg
     4441 ccctggccgc cgccctgtcc ctggccctcc tctcctcctg cgcctcggcg cccaaggaga
     4501 tcccggagga cctcacctcg cgcgagctca tccagcgcgc ccaggaggcc tccgacggcg
     4561 acaacttcgc cgccgcgatc gcctactacg aggccgcccg cgagcgctac ggctacgacc
     4621 ccgccgtcct cgccaccgcc gagtacgagg tctccttcat ctactacaag cagggcaagt
     4681 acgccgaggc ggaggcgggc ttcctcagcc tcctcgcccg ctacgacggc cccgaaggcg
     4741 agagcctccc caacaccttc aagatcctcg cggagaagct cctcccgaag gtccgcgagg
     4801 cgatgggcaa gaagtagcgc gccccggatc ccgcccgagc cggccagtag ccggtgatgg
     4861 caaagccccg cccgggatcg ggcggggctt ttcgttggtc ggcgcgagaa cagggcggga
     4921 gccagccagc ttcgcgaagt cccccaggcg caaaaaaacc gcgggcacga gcccgcggtt
     4981 ccctagcgcc aaggggcggg ggagcttacc accagccgaa ggcggtgccg cagcgggtga
     5041 ggtaccagac gaagacgatg gagacgacga cggcgtagca gaagtaccag acggagaggg
     5101 cctcgccgtc gcggcgggag aggatgccgg tgaagaggga ggcggccacg cagacgacgt
     5161 agccgatcag ggcgaggacg ccgtaggtcg tgttcggcag ggcgagggcg tcgaggcaga
     5221 acacgaaggt gaagacgaaa agaaggataa agaggccgaa gttcacgagg ccgttcttag
     5281 catccatgcg gtgactcctt tacgccggga aatcgatggt tttcgcagtc cagtataaac
     5341 ccgcccacgg gaaatgtcca ctcgcgcgag gcaaatccgg gccggctagg agacggcgga
     5401 gagctcctcg gccacgcttt cccactccgc gttgagcgct tccgcctccg cctcgagctc
     5461 ctccacggag gcctggagcc tccgcatcct ggccccgtcc gagtagttgg ccggaagccc
     5521 catctcccgc tcgcaggccg ccttctgggc gcccagggac tcgagcctgg ccaggatctc
     5581 ctcctcgcgc ttggccagct tgcggagccg cgccttcctg gccttctcct cctcgtagga
     5641 gagggcggtc gccgctccgg cggcctgggc ggcggcctcg ggccgggcgc ccgagggctg
     5701 ggcgccgcgg ccgcccgagg cggctcccgc cccgccggat ccggccgcct cggcctggcg
     5761 ctcctccgcg aggagggcct ttttctggag atagtagtcg tagttcccca cgtaccagcg
     5821 cgcgctctcg tcgggctcga gctccaggac ccggtccgcg agctcctcga tgaagccgcg
     5881 gtcgtgggag acgaagagga cggtgccctg gaaggacttg agcgcctcga ggaggacgtc
     5941 cttggaggtg aggtccaggt ggttcgtcgg ctcgtccagg atgaggaggt tggcgggctt
     6001 gaggagcatc gtgaggaggg ccaggcggct ccgctccccg ccggagagga cgcgcaccgg
     6061 cttctcgacg tcgtcgcccc ggaagaggaa ggcgccgagg aggttccgga ccttcggaag
     6121 gaggtcgaag gggcagagcg cctcgacggt ctcctccacc gtcgcgtccc cgcccatgag
     6181 gtcggcggtg tcctgggcga agtaggccgt ctccacgccc gagccgaagc ggatctcgcc
     6241 ctcgtagtcc cggtcggtcc ccgcgatgag ccgcatgagg gtggacttgc cggcgccgtt
     6301 cgggcccacg aaggcgacct tcatgccccg ctctatctcc aggtccaggc ccgagaggac
     6361 gcggtgggcg ccgtagctct tgccgacgcc caggcacttg accgtcgcct tccccgagtg
     6421 gggggccggc gggaaggaga agtggatctt cttcatgccc tcggggatct ctatcgggac
     6481 gatcttctcg agctgcttca cgcggctctg gacgagggag gccttggtcg cctggtagcg
     6541 gaaacggcgg atgaactcct cgagcttctg gatctcctcc tgctggcgct cccaggcctc
     6601 gaagaccgcc tcgagctcct cggcgcggcg ccgttcgtag gcggagtagg tgcccgcgta
     6661 gcgggtcagc ctgccgttcc agagctcgta gacctcgcgc acggtcgcgt ccaggaagta
     6721 gcggtcgtgg gagacgacga ggacgccgcc cgggtacttc gacaggaagg cctcgagcca
     6781 ctcgcgggcc tcgaggtcca ggtagttcgt cggctcgtcc aggagcatga tgtcgggatt
     6841 ctcgaggagg accttcgcga gggcgacgcg catctgccag ccgccggaga actcggcgac
     6901 gggccggtcc atctccgcgt cgcggaagcc caggcccgcg aggatctcgc ggatccgctc
     6961 gcgccgccgg aagtagcccg agccctcgac cgcctcgtgg atggccgcgt gctcctccag
     7021 gagccgggcg gtctggccgc cgtcggaggt cgagtcctcc aggaggcggc cgatctcctc
     7081 ctgcctcccg agcatcgcgg cggcgaagtc gtaggcccgc tcggcctcct cgtagaccgt
     7141 gccgccgccg tggacgacgc ccgtctgcgg aaggtaggag acgcgggcgc ccttcgtgac
     7201 ggcccgctcc ccggagtcgg gggagagctg gccggcgacg atccgcatca gggtcgtctt
     7261 cccggcgccg ttcgggccgg cgagggcggc ccgcgagccc gcggccaggt agagggtggc
     7321 gttcttgagg atgtccctgg ccccgaaggc caggctcacg tcggagaact ggacgaaggc
     7381 cacggctacc gcctcttggg cgcgaaacgg aggggcggca gggaggaggc cgagcctacc
     7441 tcgaggccgg agacgccgac cgcgggctcg agctccaggc tcccgtcgcg gaggcggaag
     7501 aggaaatccg tccagccgcc cgagccctgg tcgaagcgca gggagacggc gccgctccac
     7561 tcgccggaaa gggagggcgc gaggaagagg cggaaggccg cctcgcccgt cgccgccttg
     7621 gcggattcgc ccggaggcgc ggcgccggcg gagcccgcgg cgcccgtctg cggcgcggcg
     7681 cccgcgaccg tctccgcggg cgaggcgaag gccaggcccg gggcctcgcc cagggcggcg
     7741 gcgacgtcct cgggaaggta gcccgagggg agggccccga cgcccgtcca ggagaagcgc
     7801 ttgctccgcg agaggaccag gctggacgcg ggcgcctgcg cggccgcggg atcggggggc
     7861 tcggcgcccg agggctcgta gacccactcc tcgccgaagg ccaggaaggc gtccaggagc
     7921 ttctgccgcc tgagctcctc gtcgcggacc gcgtcccgga gctccttgga gaggatcgtg
     7981 aagacggccc ggccgtcggc gccgggcttg ggcgcctccg cgccggagtc ggcggcgggc
     8041 ccggccgagc cgggctcctc cgcctcgggc tggagggcct gggcgctccg tcccgaccag
     8101 tcggcgacca ggcggcgctc cccctcgaaa cgcacccgca agggcgtgcc ctcgaagacc
     8161 aatgccccgc cctcctcgga gagggagtca tagtcgaaga cctgggagac ggagg
//
LOCUS       DAOOSJ010000110         8195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5496_length_8195_cov_5.740049, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000110 DAOOSJ010000000
VERSION     DAOOSJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5496_length_8195_cov_5.740049"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1267)
                     /locus_tag="PLG14_06760"
     CDS             complement(53..1267)
                     /locus_tag="PLG14_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX48875.1"
                     /translation="MLLVEDEAIIALAESRLLAKAGYAVVVARSGEQAVELVRGGPEI
                     DLVLMDIDLGAGMDGTEAARLILGLRDLPLVFLSSHTEPLVVERTEGLTSYGYIVKNS
                     GETVLLASIKMAFRLFQARAELRDREKHYRSLFENAGGPVWIEDFSAFKRRVAELREA
                     GFPDFRAWLAARPEELFALADLVRVVDLNRAARASIGLAEGEPPPRSLGPFLGPEARS
                     FFAEELSVFAEGGLSFESEILVERQGSEPSVYFLSMKAIPGYEADLSRVHVLVQDLSA
                     VRRAESALKASERRNAALLSALPDLMFVFSRDGRVTDCRASDPRLLYVPAAEQIGRHV
                     SEILPSDLAELTLRKMALVLERKAPEIYDYCLVLDRPRRFESRLVPMGEESCLAIVRE
                     RGEGAATAASGR"
     gene            1481..2725
                     /locus_tag="PLG14_06765"
     CDS             1481..2725
                     /locus_tag="PLG14_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48876.1"
                     /translation="MENASPTARPNPLARLVAPYRGLGGSLWSMFFATMINRFGDFVG
                     AFLALYLSRILKLDAVRTGTTISLVFAASMVGSLLSGRIADAVGRKRALLICQGSAAA
                     VNMAMSFLYGFEWAPLLIVAGSLFRGGARPLIGAVLTDLAPAGKRKEVFGIQYWSINV
                     GVAVGPLVAAFLFDRSLPWLFRGDALSTFASVALIARGVKMPAAATASTSLERHDERG
                     AMAAFVARPILIAFAGLAFLSSLSYCQTGFGLPLRVSEAFAEGGPRFMGYLMSLNAVL
                     VIVLSIPLARLLRSRTPLACMAYSGGFYVVGFGLLAFPLGKAGFVASTAVWTIGEIVS
                     SINMGVFLAKHSPANWRASFQSFMGVFYSAGWTVGPLVAGPLVRGAGMPALWTATAAL
                     CALWAAGAVAVDGWDRRIAPER"
     gene            2857..>3674
                     /locus_tag="PLG14_06770"
     CDS             2857..>3674
                     /locus_tag="PLG14_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOX48877.1"
                     /translation="MIHWESREGTRLPIKSWCREVEPKALAQALSLANHPALFHHVAL
                     MPDCHVGFGMPIGGVVAARDAVIPNAVGVDIGCGMRAARTTVASDRVLDKALLRAVVE
                     RVKEAVPVGEGRARRLPLGWSGFEEYRDALAASGGEPGWLEARGWELDALNLGTLGGG
                     NHFIELDRGDDGRAWLMLHSGSRNLGYRIANYYHRAALDLDLRRRLELPDRELAYLPL
                     DEELGRAYLRDMDFALRYAAESRRLMMEALKEAFASLVPGAGFDLELDIHHNYAA"
     assembly_gap    3675..3774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4003..4260)
                     /locus_tag="PLG14_06775"
     CDS             complement(4003..4260)
                     /locus_tag="PLG14_06775"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HOX48878.1"
                     /translation="MNKAIINILEVTAPSAQAFGAVRLSKGQAASLSPRSGSRLVCAE
                     GSLWLTREGDPADYILKAGESFELDAPLVVSGLGSASYLVA"
     gene            4483..>5899
                     /locus_tag="PLG14_06780"
     CDS             4483..>5899
                     /locus_tag="PLG14_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX48879.1"
                     /translation="MEGKRPLYERFARELEAQIQAGTFPEGSRLPALRESSESRGISL
                     STVIQAYRLLETKGLIEARPQSGYYVKRRQRRRVLEAAAEPEPLRETPSSVSIDELSR
                     DLFRDTHDPDVAQFGAAAPDPELLPTARLNRILAELAREGRFALDTSVYPEGCEELRV
                     QIAQRAFSAGCPLRPDKILVTAGCTEAMALALQALCRPGDLVAIESPSYFGVLLLLEA
                     LGLRALEIPANPGTGLSLEALEFALENHPVKAVLAVPNFSNPLGSLMPDESKRRLVGL
                     LAERGIPLIENDIYGELYFGDKRPSVARSFDPEGSVLLCSSFSKDISPGYRIGWVAAG
                     RRFEELFKLKMAISSGTSVLPQLTIARYLESGGYDLHLRKIRRAYARKVAEMSRAIEE
                     GFPEGTLVSEPEGGYLLWVRLPSGLDSMELYREALASRIAIAPGYLFSPSHKHKDFIR
                     LNAGIWSERSAKDLARLGRLVE"
     assembly_gap    5900..5999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6000..7930)
                     /locus_tag="PLG14_06785"
     CDS             complement(<6000..7930)
                     /locus_tag="PLG14_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX48880.1"
                     /translation="MEFTELNLFPDLQRGLEEAGYVTCMPVQEKVFEHSFSGDDIYAQ
                     SQTGTGKTAAFLISIFQRIMTVPEVRDRKVLILAPTRELAVQIEGEAKKLGKHLPISS
                     GAFYGGVAYGPQVDMLRDNVQVIIGTPGRIIDFVNQGKMLLKDVGFLVIDEADRMFDM
                     GFIDDLRKLIRYLPPAGNRQTYLFSATLGLRVKDLAWEYMHEPHEIAIEPEHVTVDLV
                     TQELYHVGSHEKMRLLLGVLDREKPKSCIVFCNQKFMVEEVARRLKINGFDCEYLMGD
                     LPQSQRLRIIERLKAGELSLLVATDVAARGLDVEALDLVVNYDVPDEVESYVHRIGRT
                     ARAGKAGKAVTLACEKFVFGIPSIEKYLGQKIPVAPFDTALLLEDRSEGMRFGRTYGG
                     PGDRGGRGGDRGRSGGYGDRGRSGGRAPSGGGYGHREGPRREDTRREGPRREGAYAEG
                     GRDTRSEERRDGERPENRENRSHFEDRGIRSGERRPADRRPSDRRSGERRDDTRRDEA
                     RREGQGEGRGQRSAQRPGSQARPGAPAGRDPYALSSEERMKSYKDKYANGDRGQGGKP
                     AEGGSERGGQGGRKAQRPDIRRGAAQPPREAARKPAPHGSDRAPHQKGSPAAAQASGL
                     KKPGLVERLKGLFRGKGRE"
     gene            complement(7982..>8195)
                     /locus_tag="PLG14_06790"
     CDS             complement(7982..>8195)
                     /locus_tag="PLG14_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48881.1"
                     /translation="LPAESPTARAGRAGAAYRAEDARAGAPGFRLAAGSSAAAEARAP
                     GFDEGKAFVAARSFAAAEDLAPPPYY"
BASE COUNT         1127 a         2835 c         2791 g         1242 t
ORIGIN      
        1 agcgagcagg tgggcggaga gctcgaggtc gagggcaggc gggggacgag gatcagcgtc
       61 cgcttgccgc tgtagccgcc ccttcccccc tctcccgcac gatcgcgaga cagctttcct
      121 ctcccatcgg cacgaggcgg ctctcgaagc ggcgcggccg gtccaggacg aggcagtagt
      181 cgtagatctc cggcgccttc cgctccagga ccagggccat cttccgcagg gtgagctcgg
      241 ccaggtcgga cggcaggatc tcggagacgt gccggccgat ctgctccgcc gcgggcacgt
      301 agaggaggcg gggatcggag gcccggcagt ccgtcacgcg gccgtcccgc gagaagacga
      361 acatgaggtc gggcagggcc gagagaaggg ccgcgttccg ccgctcgctc gccttgagcg
      421 ccgactcggc ccgccggacc gcgctcaggt cctggactag gacgtggacg cgcgagaggt
      481 cggcctcgta gccggggatg gccttcatgc tgaggaagta gacgctcggc tccgaaccct
      541 gtctctcgac gaggatctcc gattcgaagg acaggccccc ttccgcgaag accgagagct
      601 cctccgcgaa gaaggaacgg gcctccggcc ccaggaaggg cccgagcgac ctaggcgggg
      661 gctcgccctc ggccaggccg atggaggctc gggcggcgcg gttcaggtcg accacgcgga
      721 ccaggtcggc cagggcgaag agctcctcgg ggcgcgcggc gagccaggcg cgaaaatcgg
      781 ggaagccggc ctcgcggagc tcggccacgc ggcgcttgaa ggcggagaag tcctcgatcc
      841 agaccgggcc gcccgcgttc tcgaagaggg agcggtagtg cttctcgcgg tcccgcagct
      901 cggcgcgcgc ctggaagagg cggaaggcca tcttgatgga ggcgaggagg acagtctcgc
      961 ccgagttctt gacgatgtag ccgtaggagg tgaggccctc cgtccgttcg acgaccaggg
     1021 gctcggtgtg cgaggagagg aagacgaggg gaaggtccct gagtccgagg atgaggcgcg
     1081 cggcctccgt gccgtccatg ccggccccca ggtcgatgtc catgagtacg aggtcgattt
     1141 ccgggccgcc ccggacgagc tcgactgcct gttcccccga gcgggccacg acgaccgcgt
     1201 agcccgcctt cgcgaggagc cgggactcgg cgagggcgat gatcgcctcg tcctcgacga
     1261 ggaggatggt cttggcgggc taaggcttgc ggcccgcttc cggccgcgtc tcggggctca
     1321 tcttcgcagt atagggccgg gacctccccg gggcaagcga aagaggaggc ccggcggccc
     1381 gcgccgggat cgaagccgcg gcaggcggcg tggtacgcgc ctcggcgatc gccgcgccga
     1441 cgcctcgacg cggagggccg ccgcggatag aatccccccg atggaaaacg caagcccgac
     1501 cgccaggccc aacccgctcg cgcgcctcgt cgccccctac cggggcctcg ggggctcgct
     1561 ctggtccatg ttcttcgcga cgatgatcaa ccgcttcggc gacttcgtcg gcgccttcct
     1621 cgcgctctac ctctccagga tcctgaagct cgacgcggtg cgcacgggca cgaccatctc
     1681 cctcgtcttc gccgcctcca tggtcggctc cctcctctcg ggccgcatcg ccgacgccgt
     1741 ggggcggaag cgggccctcc tcatctgcca gggctcggcc gccgcggtca acatggcgat
     1801 gagcttcctc tacggcttcg agtgggcccc cctcctcatc gtcgcgggca gcctcttccg
     1861 cggcggcgcc cgacccctca tcggcgccgt cctcacggac ctggcgccgg cgggaaagcg
     1921 gaaggaggtc ttcggcatcc agtactggtc gatcaacgtc ggcgtggccg tcggccccct
     1981 ggtcgcggcc ttcctcttcg accggagcct cccctggctc ttccgcggcg acgcgctcag
     2041 caccttcgcc tccgtggccc tcatcgcccg cggcgtgaag atgccagccg ccgcgacggc
     2101 ctccacctcc ctggagcgcc acgacgagcg cggcgcgatg gccgccttcg tcgctcggcc
     2161 catcctcatc gccttcgcgg gactcgcctt cctctcgagc ctctcctact gccagacggg
     2221 cttcggcctg cccctccgcg tctcggaggc cttcgcggag ggcggcccgc gcttcatggg
     2281 ctacctcatg tcgctcaacg ccgtcctcgt catcgtcctc tcgatccccc tcgcccggct
     2341 gctccgctcc cggacgcccc tggcctgcat ggcgtactcg ggcggcttct acgtcgtcgg
     2401 cttcggcctc ctcgccttcc ccctgggcaa ggccggcttc gtcgcctcca cggccgtctg
     2461 gacgatcggc gagatcgtct cctccatcaa catgggcgtc ttcctggcca agcactcgcc
     2521 cgcgaactgg cgggcctcct tccagtcctt catgggcgtc ttctactccg cgggctggac
     2581 cgtcggcccc ctggtcgccg gccccctggt caggggggcg ggcatgcccg cgctctggac
     2641 ggcgaccgcg gcgctctgcg ccctctgggc cgcgggggcc gtcgcggtgg acggctggga
     2701 caggcggatc gcccccgagc gctagacggg gaggaccccc ctttcattcc gggtgaaagc
     2761 gcccatggcg cgaaagccac agaggggcgt gaaagccaac gcgagcctcg cgcgcaggaa
     2821 gtcccgggac tagacttcca gtgagggggc gcacacatga tccactggga aagccgagaa
     2881 gggacgaggc tccccatcaa gtcctggtgc cgagaggtcg agcccaaggc cctcgcccag
     2941 gcgctaagcc tggcgaacca ccccgccctc ttccaccacg tcgccctcat gcccgactgc
     3001 cacgtcggct tcggcatgcc catcggcggg gtcgtcgcgg ccagggacgc ggtgatcccg
     3061 aacgcggtgg gcgtcgacat aggctgcggg atgcgggcgg cgcggacgac ggtcgcctcg
     3121 gaccgggtcc tcgacaaggc cctgctccgc gccgtcgtcg agcgggtgaa ggaggccgtg
     3181 cccgtcggcg agggccgcgc gcggcgcctc cccttgggct ggagcggctt cgaggagtac
     3241 cgcgacgcgc tcgccgcctc ggggggcgag ccgggctggc tggaggcgcg gggctgggag
     3301 ctcgacgcgc tcaacctcgg caccctgggc ggagggaacc acttcatcga gctggaccgg
     3361 ggggacgacg gccgagcctg gctcatgctc cactcgggct cgaggaacct gggctacagg
     3421 atcgccaact actaccaccg cgccgccctg gacctggacc tgcggaggcg cctcgagctc
     3481 cccgaccggg agctcgccta cctgcccctg gacgaggagc tcggccgggc ctacctgcgc
     3541 gacatggact tcgccctgcg ctacgccgcc gagagccgcc gcctcatgat ggaggccctg
     3601 aaggaggcct tcgcctcact ggtgcccggc gccggcttcg acctggagct ggacatccac
     3661 cacaactacg cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcttc
     3781 ggccccgcgc ggctccgcgg ggcgaagggc ggcaggggcc tcctcgactt gtccgaggct
     3841 ccgcaggcct acaaggacat cgaggaggtc ctggcctcgg agctggacct ggtaaggccc
     3901 ctggtcaggc tggaggcgct cgccgtcgtg aagggctagg gcctgtcggg gccggcgacg
     3961 cggggccggg gaccggcgga ccggcgatcc gaggtacggc cctcaggcga cgaggtagga
     4021 ggccgagccg aggccggaga cgacgagggg agcgtcgagc tcgaagctct cgccggcctt
     4081 gaggatgtag tccgcgggat cgccctcgcg ggtgagccag aggctcccct cggcgcagac
     4141 gaggcgcgag cccgagcggg gcgaaaggct cgcggcctgg cccttggaaa ggcgcacggc
     4201 gccgaaggcc tgggcggaag gggcggtgac ctcgagaata ttgatgatgg ccttgttcat
     4261 aggctcctcc ttgggacttg cgaaaggcgg ctgtctggtc cgcagggaga atatagtgaa
     4321 tttacctaga aacataacag agctagatta tcgaaattat atacataaca gattgcatca
     4381 agctcaactg ttatggctat aaatggagaa aactgtattg ttttctaaat acaattcaag
     4441 atcgcgcttg ctattccagc agaatgccat aacaatatgt tcatggaagg caagcgcccc
     4501 ctctacgaac gctttgcccg cgagctggaa gcgcagatcc aggcgggaac ctttcccgaa
     4561 ggcagtcgcc ttcccgccct ccgcgagtcg agcgaaagcc gcggcatctc cctctccacc
     4621 gtcatccagg cctaccgact cctggagacc aagggcctca tcgaggcgcg gccccagtcg
     4681 ggctactacg tgaagcgccg ccagcgccgg cgcgtcctgg aggcggcggc cgagcccgag
     4741 cccctgcgcg agacgccgag ctcggtgagc atcgacgagc tctcccgcga ccttttccgc
     4801 gacacccacg accccgacgt ggcccagttc ggggccgccg cccccgaccc cgagctcctc
     4861 ccgacggccc gcctgaaccg catcctggcc gagctggccc gcgagggccg cttcgccctg
     4921 gacacctccg tctaccccga gggctgcgag gagctccgcg tccagatagc ccagcgcgcc
     4981 ttctccgccg gctgccccct gcggcccgac aagatcctcg tcacggccgg ctgcacggag
     5041 gccatggccc tggccctcca ggccctctgc aggccgggtg acctcgtcgc catcgagtcg
     5101 ccctcctact tcggcgtcct cctcctcctc gaggccctgg gcctccgcgc cctggagatc
     5161 ccagccaacc cgggcacggg cctctccctc gaggccctgg agttcgcgct ggagaaccat
     5221 cccgtgaagg ccgtcctcgc ggtgccgaac ttctcgaacc ccctgggaag cctcatgccc
     5281 gacgagtcca agcgccgcct cgtcgggctc ctggccgagc gggggatccc cctcatcgag
     5341 aacgacatct acggggagct ctacttcggg gacaagcggc cgagcgtggc ccgctccttc
     5401 gatcccgagg gctcggtcct cctctgctcc tccttctcca aggacatctc gcccggctac
     5461 cgcatcggct gggtcgccgc ggggcgccgc ttcgaggagc tcttcaagct caagatggcg
     5521 atcagctcag gcacctcggt cctgccccag ctcacgatag ctcgctacct ggagtccggc
     5581 ggctacgacc tccacctgcg gaagatcagg cgagcctacg cgcgcaaggt ggcggagatg
     5641 tcgcgggcga tcgaggaggg cttccccgag ggcaccctgg tctccgagcc cgagggcggc
     5701 tacctcctct gggtgcgcct gccatccggc ctcgactcga tggagctcta ccgcgaggcc
     5761 ctggcctcgc gcatcgcgat agcgccgggc tacctcttct cgccctcgca caagcacaag
     5821 gacttcatcc gcctcaacgc cgggatctgg tcggagcgga gcgcgaagga cctggcgcgc
     5881 ctggggcgcc tggtcgagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     6001 cctcgcggcc cttcccgcgg aagagcccct tgaggcgctc gaccaggccg ggcttcttga
     6061 ggccggaggc ctgggcggcc gcgggcgagc ccttctggtg gggcgcccgg tccgagccgt
     6121 ggggcgcggg cttgcgggcc gcctcgcggg gaggctgggc cgcgccgcgg cggatgtcgg
     6181 gccgctgggc cttccggccg ccctggccgc cgcgctcgga gccgccctcg gcgggcttgc
     6241 cgccctggcc gcggtcgccg tttgcgtact tgtccttgta gctcttcatg cgctcctcgc
     6301 tcgagagggc gtaggggtcg cggccggcgg gggcgccggg cctggcctgg gagcccggcc
     6361 tctgagcgga gcgctggccc ctgccctcgc cctggccctc gcgccgcgct tcatcgcgcc
     6421 gcgtatcgtc gcgccgctcg ccggaacggc ggtcgctcgg cctgcggtcg gcgggcctgc
     6481 gctcgcccga gcggatgccg cggtcctcga agtgggagcg gttctcgcgg ttctccggac
     6541 gctcgccgtc gcggcgctcc tccgacctcg tgtcgcgtcc gccctcggcg taggcgccct
     6601 cgcgcctcgg accctcgcgc cgcgtatcct cgcgccgcgg accttcgcga tggccgtagc
     6661 cgccgcccga gggggccctg ccgccggagc ggcccctgtc gccgtagccg ccggagcgcc
     6721 cgcggtcgcc gccgcgcccg ccgcggtcgc ccgggccgcc gtaggtgcgg ccgaagcgca
     6781 tgccctcgct ccggtcctcc aggaggaggg ccgtgtcgaa gggggccacg gggatcttct
     6841 ggcccaggta cttctcgatg gaggggatgc cgaagacgaa cttctcgcag gccagggtca
     6901 cggccttgcc ggccttgccc gcgcgggccg tgcggccgat gcggtgcacg taggactcga
     6961 cctcgtcggg cacgtcgtag ttcacgacca ggtccagggc ctcgacgtcc aggccgcggg
     7021 ccgcgacgtc cgtcgcgacc aggagggaga gctccccggc cttgagccgc tcgatgatcc
     7081 tgaggcgctg ggactgggga aggtcgccca tgaggtattc gcagtcgaag ccgttgatct
     7141 tgaggcgccg cgcgacctcc tcgaccatga atttctggtt gcagaagacg atgcagctct
     7201 tgggcttctc gcggtccagg accccgagga ggaggcgcat cttctcgtgg ctgcccacgt
     7261 ggtagagctc ctgggtgacg aggtcgaccg tcacgtgctc gggctcgatg gcgatctcgt
     7321 ggggctcgtg catgtactcc caggccaggt ccttgaccct gaggccgagg gtcgccgaga
     7381 agaggtaggt ctggcggttg cccgcgggcg gcaggtagcg gatgagcttg cgcaggtcgt
     7441 cgatgaagcc catgtcgaac atgcggtccg cctcgtcgat gaccaggaag ccgacgtcct
     7501 tgaggagcat cttgccctgg ttcacgaagt cgatgatgcg gccgggcgtc ccgatgatca
     7561 cctggacgtt gtcccggagc atgtccacct gggggccgta ggcgacgccg ccgtagaagg
     7621 cgccggagga gatcgggagg tgcttcccga gcttcttcgc ctcgccctcg atctggacgg
     7681 cgagctcgcg cgtgggcgcg aggatgagga ccttgcggtc gcgcacctcg gggacggtca
     7741 tgatgcgctg gaagatcgag atgaggaagg cggccgtctt gccggtgccc gtctgggact
     7801 gggcgtagat gtcgtcgccg gagaaggagt gctcgaagac cttctcctgg acgggcatgc
     7861 aggtcacgta gccggcctcc tcgaggccgc gctggaggtc ggggaagaga ttgagttcgg
     7921 tgaattccat ggtctcgctc ggtatggtca gattgttggc ttagaatact tcattttcga
     7981 ctcaataata agggggtggc gccagatcct ccgcggcagc gaaggatctg gcggcgacga
     8041 aggctttgcc ttcgtcgaat cccggggctc gcgcctccgc ggcagcggag gatccggcgg
     8101 cgaggcgaaa gccgggcgcg ccggcgcggg cgtcttcggc gcgataagcg gcgccggccc
     8161 ttcccgcccg cgccgtcggg ctttccgcgg gtagc
//
LOCUS       DAOOSJ010000111         8170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5519_length_8170_cov_6.263216, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000111 DAOOSJ010000000
VERSION     DAOOSJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_8170_cov_6.263216"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLG14_06795"
     CDS             <1..263
                     /locus_tag="PLG14_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48882.1"
                     /translation="YLQLYRSDDDVNMERVLDAKLEERIADRNLVEVLYDPETGYLAV
                     ALNGRLAAKYRTWFGIEGGIGVSLRSLGGGASFSGFEVLAAR"
     gene            579..1625
                     /locus_tag="PLG14_06800"
     CDS             579..1625
                     /locus_tag="PLG14_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48883.1"
                     /translation="MAGSALAAIFAAAAVLAACLVEPAPGLLPEADYRPPALLSAGAA
                     GERLFLLGFDEEIRPVEGSFALARSAAAGAAGGSAEEVPGSGPPEAVAEGAGLSVLFS
                     ESTSPGADYLLSGEVADLRGNTTRFVLGFVGWNPRPPRLRLSEFQTAKNSSLSNSHRD
                     YAELEVLADGNLGGVELAYASSVKAYRFRFPAAEVRAGDFVVLHLAPEGLPEELDETG
                     SELGLSGGVDARPYARDFWSAAGGLPDESGAAALYERPGGGLMGGFFYANAEKAGPVG
                     SGRLGELLSGLSAAGAWPLAGGEPLWEDAFLWKPSSARSLCRSPGSEEAGPGAWYLTA
                     AGGQSPGAANPPPP"
     gene            complement(1783..2106)
                     /gene="trxA"
                     /locus_tag="PLG14_06805"
     CDS             complement(1783..2106)
                     /gene="trxA"
                     /locus_tag="PLG14_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOX48884.1"
                     /translation="MSAEIVVTAENFDSVVLASDKPVLLDFWAEWCMPCRMIAPSIEQ
                     LAESYAGRLVVGKVDVDSQSELAGRFGIISIPTMIVFKGGEVARQKVGALPKHEIEAL
                     FKDLL"
     gene            2309..2977
                     /locus_tag="PLG14_06810"
     CDS             2309..2977
                     /locus_tag="PLG14_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX48885.1"
                     /translation="MTDEDREARWTGTFARLPSDKRARVLEAAKRAFAANGYSGANVN
                     KIAAEAGISVGSMYKYFRTKEDLFLALIEDFHRLIESSIDAILAREPRFLARVEALLR
                     AAAETSAADPENVRIYICCTTEELAPLAARLSGNIEAASAGRYRAMVVEAQAKGEVDP
                     SVDPAWAAFFLDDLFLLLQYSYGSEYYRERLRVFLGVPELPDPGTAVARLLGLAARAL
                     GAVA"
     gene            complement(3053..4306)
                     /locus_tag="PLG14_06815"
     CDS             complement(3053..4306)
                     /locus_tag="PLG14_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX48886.1"
                     /translation="MSETRYRVYGYRWAVLAAAMAVNFTIQTLWISYAPVTGAAAAFY
                     GVSELKVGLFSMAFMLAFIPLSIPVAWLIDRFGFALVVGLGSAATGAFGLLRGLAGAD
                     YGLALLATCGMAAAQPCLLNAWTKMPAAWFPARERATAVGLTTIANLGGTALGLVLTP
                     VLAASLPLPRIQLAYGAAAAASALAFVLVARERPATPPDESASRERALMVEGLRHALS
                     VPSFRRYLLLAFVGMGVFNGVTTWVEAIVRPRGVAPAEAGLLGALMLAGGLAGAALLP
                     ALSDRRGRRKPFIALGLAAAAPALAGLAFAPSYPLMAAAALLLGFFLVAVSPIGMQYA
                     AEAARPTPEGSSNGLVSLAGQASVLLVYAMEALNAATGSFAASLSASALLLLLSGLLA
                     AGLAEGSPSAAPEGAEARAPSGPRG"
     gene            complement(4303..5931)
                     /locus_tag="PLG14_06820"
     CDS             complement(4303..5931)
                     /locus_tag="PLG14_06820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOX48887.1"
                     /translation="MSDAHVLAVDVGTTSAKSCLYRLPEAGGSLELVACAGAEYAIRI
                     LPDGGAEQDPEDWWRAAAGGARAALAAAGLPPSAVRGMAFCCQMQGLVLVDSGGRPVR
                     PAMSYMDQRAREEKRRGLERGLKVEGMGLSRLLPSLYLAGGVSASAKDPVWKYHWVRA
                     HEPEAFARAAKWLDVKEYLVARATGRCAMTPDSANATFLYDTRPGRLGWSPLLARLFD
                     VDSRHLPEVIGASEAAGPLLPGAAAELGLEAGTPVFGGGGDLSMIALGSGAVGLGRAH
                     VYMGTSGWVASSVDRRVVDTKARAASVLGASPGRYNYISEQETSGKCMEWVRDHLALD
                     EIGLYLGARHVCEDPEARYASVFELLDETVESVPPGSGGVVFAPWLRGSRSPFEDPDA
                     RGVFFNIGLDTGKRCLVRAVAEGIALQSRWQLEAIRRKVEARGPLRFVGGGARSRAAA
                     RVLADVLGETVETTASPQNAGALGAAIVCAAGLGALSSLEDAAGLVPAAAAYEPRPER
                     RELYDLRYEVLKRLYYDNRESFKALNAPAQRKER"
     gene            complement(5928..6641)
                     /locus_tag="PLG14_06825"
     CDS             complement(5928..6641)
                     /locus_tag="PLG14_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOX48888.1"
                     /translation="MDEGEAREAVRRAGVELVDTGLVDGTWGNLSIRFKGPDGRSLMA
                     ITPSGRDYRSLGPGDIVLVDLATGEASGGKPSTETPLHRAIYAARPEVGAIVHTHSAS
                     ASTVAAARREVPPVLDDFAQVVGPSLRVADYALPGSGRMARVVVKALRGRMAALLANH
                     GAVALGRSMAEALLCARMAEKGCRVFIEAEFLGGAKPLAKVEAWAMHEVYLRKYSKLG
                     AAAGGVPGGESRTGGRGER"
     gene            complement(6647..8116)
                     /locus_tag="PLG14_06830"
     CDS             complement(6647..8116)
                     /locus_tag="PLG14_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX48889.1"
                     /translation="MNYSISAYPDAAEIYGRLARLVAEPPRPIARPAMEAYLERLDRE
                     CPRSREIVAEAKRYIPGGVQHNLAFNYPFPLVIDRAEGAWLHDVDGRRYFDFLQAGGP
                     TILGSNDPEVRRQVLEVLEKVGPSTGLFHEFELALAKEIGRHMPSVEMFRMLGSGTES
                     VMAAIRVARLATGKQRVVKAGGAYHGWSDQLVYSLKVPKTRRYEAHGIPRAMSSLTDE
                     AAPNDLSSLERVLRANRLRGGTACVLLEPLGPESGTRPVDRDYNSGARALCDKYGALL
                     VFDEVVTAFRVGLGGAQGYFGVRPDLTTFGKIVAGGYPSAGGLGGRRDLVLLMAAGLE
                     SGKRRAYVGGTMAANPLSAAAGYFTLREIEKRDACAAAGRAGDRLTAGLRRRIEELGL
                     PFVAYNQGSICHLETVGAMFVPMDYLRLRKTLAELKERKRLMEEYGAAYAAEGLVTLA
                     GSRMYTSAADTDEVVDEAVERFGRVFESVEAPRAAAGRE"
BASE COUNT         1086 a         2901 c         3067 g         1116 t
ORIGIN      
        1 cctacctcca gctctaccgc tccgacgacg acgtcaacat ggagcgggtc ctggacgcca
       61 agctcgagga gaggatagcc gaccgcaacc tcgtcgaggt gctctacgat cccgagaccg
      121 gctatctcgc cgtggcgctc aacggccgcc tggccgcgaa gtaccgcacc tggttcggca
      181 tcgagggggg catcggcgtc tccctgcgca gcctgggcgg cggcgcctcc ttctcgggct
      241 tcgaggtcct ggcggcccgc taggcttctt ttctgcgccg cgcctttgcg tcctgcctgt
      301 ccggggccgg gcgcccgggc gggcaggacg cctgcgctag aaatgccgcc tgcgctcgac
      361 caggtcgcct gcgctcgacc aggtcgccgg cccgcgctca atcgggccgg ctggccgcgc
      421 gcgttcctcc ttcggcgaag gaggaggaac cgatgggtga actcgagggg gaggtccgcg
      481 aggggccggc ccgccgtccc tggaggcgtc ccggccgcgg cctggccgcg ggtggcctgg
      541 ccgcgggtgg ccgcgtggcg agtggcctat gtctgccttt ggctggctca gccctggccg
      601 ccatattcgc cgcggccgct gtcctggcgg cctgcctggt cgagcccgcg ccgggcctcc
      661 tgcccgaggc cgactaccgt ccgcctgccc tcctctccgc cggagcggcg ggggagcggc
      721 tcttcctcct cggcttcgac gaggagatca ggcccgtgga gggcagcttc gccttggctc
      781 ggtccgctgc cgcgggcgcg gccggcggct ccgcggagga ggttccggga tcgggcccgc
      841 ccgaggcggt cgccgaaggc gcggggctca gcgtgctttt ttccgagtcc acgagccccg
      901 gcgcggacta tctcctctca ggcgaggtcg ccgacctgcg cgggaacacg acgcgcttcg
      961 tcctcggctt cgtcggctgg aaccccaggc cgccccgcct gcggctctcc gagttccaga
     1021 ccgcgaagaa cagctccctt tccaacagcc atcgggacta cgccgagctc gaggtcctcg
     1081 cggacggcaa cctgggcggg gtcgagctgg cctacgcgag ctccgtgaag gcctatcgct
     1141 tccgcttccc cgccgccgag gtccgggccg gcgactttgt cgtcctccac ctcgcgccgg
     1201 agggcctgcc cgaggagctg gacgagacgg ggagcgagct cggcctctcg gggggcgtcg
     1261 acgcgaggcc ctacgccagg gacttctggt ccgcggcagg cggcctaccg gacgagagcg
     1321 gagcggcggc cctctacgaa aggccgggcg gcgggctcat gggcggcttt ttctacgcga
     1381 acgccgagaa ggccgggccc gtgggctcgg ggcgcctcgg cgagcttctc tccgggcttt
     1441 cggccgcggg agcctggccc ttagcgggcg gcgagccgct ttgggaggac gcattcctct
     1501 ggaagccctc gtcggcccgc tcgctttgcc gctctccggg ctcggaggaa gcgggaccag
     1561 gggcctggta cctcacggcc gcgggaggcc agagccctgg cgcggccaat ccgccgccgc
     1621 cctaggaagg cggccgggcc aaggtagccg ctcggggccg ggaatagcaa aggggcgaag
     1681 aagcgaagag gcggccgggg ggccgcctct tcttcccgct tttttcggcc gcggagcggt
     1741 ccggcgggct caggcttctc gccaaggccc tggcggctcg ggctagagga ggtccttgaa
     1801 gagggcctcg atctcgtgct tggggagggc tccgaccttc tggcgggcga cctcgccgcc
     1861 cttgaagacg atcatggtcg gtatggagat gatgccgaaa cggccggcga gctcgctctg
     1921 gctgtcgacg tcgaccttgc cgacgacgag cctccctgcg tagctctccg cgagctgctc
     1981 gatggagggg gcgatcatgc ggcagggcat gcaccactcg gcccagaaat cgaggaggac
     2041 gggcttgtcc gaagcgagga ccacgctgtc gaagttctcg gcggtgacga caatctctgc
     2101 gctcatggtt cgctccttgg cgcccactgt cctcgggcgg cgatagggaa atatacgcaa
     2161 aagggctttc cctgtcaaac gcgggatccg cggaggggca tccggacagg gtctcggggc
     2221 ttccagcctg ggcaacggag cttccgggca ggcctgcggg aagcgcgctc ccttgcgccg
     2281 cggcccgccg ccggactatg ctcggcccat gaccgatgaa gacagggagg cgaggtggac
     2341 cggcaccttc gcgcggctgc ccagcgacaa gcgcgcgcgc gtgcttgagg ccgccaagcg
     2401 ggccttcgcc gccaacggct attccggcgc caacgtcaac aagatcgccg ccgaggccgg
     2461 catctccgtc ggctcgatgt acaagtattt ccgcaccaaa gaagaccttt tcctggccct
     2521 catcgaggac ttccaccgcc tcatcgagtc gagcatcgac gcgatcctgg cccgggagcc
     2581 ccgcttcctg gcccgggtgg aggccctcct gcgcgccgcg gccgagactt cggccgccga
     2641 tcccgagaac gtccgcatct acatctgctg cacgaccgag gagctcgcgc ctctcgccgc
     2701 gcggctatcg gggaacatcg aggccgcctc cgccgggcgc tatcgcgcga tggtggtgga
     2761 ggcccaggcc aagggcgagg tcgatccctc cgtcgatccc gcctgggccg ccttcttcct
     2821 agacgacctc ttcctcctcc tgcagtactc ctacggctcc gagtactacc gcgagcggct
     2881 ccgcgtcttc ctgggcgttc cggagctccc cgacccgggc acggcggtcg cgcgcctcct
     2941 cgggctcgcg gcgcgggccc tcggcgcagt cgcctaacgc ttccttcctt ccctccgcgc
     3001 cgccgcgccc tccgggggcg tcgccggcgc ggccgcacgc gccgccgaag gcctagccgc
     3061 gcgggccgga cggggcgcgg gcctccgcgc cctccggcgc ggcggacggc gagccctcgg
     3121 ccagcccggc cgcgagcagg cccgagagga ggaggaggag ggcggaggcc gagagggagg
     3181 ccgcgaagga gcccgtggcc gcgttgagcg cctccatcgc gtagacgagg aggacggagg
     3241 cctggcccgc gaggctcacg aggccgttgg agctgccctc cggcgtgggg cgggcggcct
     3301 cggcggcgta ctgcatgcct atggggctca ccgcgacgag gaagaagccg aggaggaggg
     3361 ccgccgcggc catgaggggg tagctgggcg cgaaggcgag gcccgcgagg gcgggggccg
     3421 ccgcggccag gcctagggcg atgaagggct ttctcctccc gcgcctgtcg gagagggcgg
     3481 ggaggagggc cgcgcccgcg aggccgcccg cgagcatgag ggcgccgagg agtccggcct
     3541 cggcgggcgc gacgccgcgg ggccggacga tcgcctccac ccaggtcgtg acgccgttga
     3601 agacccccat gccgacgaag gccaggagga ggtagcggcg gaacgagggc acggagaggg
     3661 cgtgcctgag gccctcgacc atgagggccc gctcgcgcga ggcgctctcg tcggggggcg
     3721 tcgcgggccg ctcgcgggcg acgaggacga aggcgagggc cgaggccgcg gcggcggcgc
     3781 cgtaggcgag ctggatccgg ggcaggggca gggaggcggc caggaccggc gtgaggacga
     3841 ggccgagggc cgtgcccccg aggttcgcga tggtcgtgag gccgacggcg gtcgcccgct
     3901 cgcgcgccgg gaaccaggcc gcgggcatct tggtccaggc gttgaggagg cagggctggg
     3961 ccgccgccat cccgcaggtc gcgaggaggg cgaggccgta gtccgcgccg gcgaggccgc
     4021 ggaggaggcc gaaagccccc gtcgcggccg agcccaggcc gacgaccagc gcgaagccga
     4081 agcggtctat gagccaggcc accgggatcg agagggggat gaaggccagc atgaaggcca
     4141 tggagaagag gcccaccttg agctcggaga cgccgtagaa ggccgccgcc gccccggtca
     4201 cgggggcgta ggagatccag agcgtctgga tcgtgaagtt gaccgccatc gccgccgcga
     4261 gcaccgccca gcggtagccg tagacccggt atcgtgtctc gctcatcgtt ccttcctttg
     4321 cgcaggcgca ttgagcgcct tgaagctctc ccggttgtcg tagtacagcc gcttgagcac
     4381 ctcgtagcgc aggtcgtaga gctccctgcg ctcgggccgc ggctcgtagg ccgccgcggc
     4441 ggggacgagg cccgccgcgt cctccagcga tgatagcgcc cccaggcccg cggcgcaaac
     4501 gatggccgcg cccagggcgc cggcgttctg cggcgaggcc gtggtctcga cggtctcgcc
     4561 caggacgtcg gccaggactc gcgccgccgc gcgcgagcgg gctcccccgc ccacgaagcg
     4621 cagggggccc ctcgcctcga ccttgcgccg gatcgcctcg agctgccagc ggctctgcag
     4681 ggcgatgccc tccgcgacgg cgcgcacgag gcagcgcttc ccggtgtcga ggccgatgtt
     4741 gaagaagacg ccgcgcgcgt ccgggtcctc gaagggcgag cggctgccgc ggagccaggg
     4801 cgcgaagacg acgccgccgg agcccggcgg gaccgactcg acggtctcgt cgaggagctc
     4861 gaagacggag gcgtagcgcg cctccggatc ctcgcagacg tggcgtgcgc ccaggtagag
     4921 gccgatctcg tccagggcga ggtggtccct cacccattcc atgcacttgc cggaggtctc
     4981 ctgctcggag atgtagttgt agcgtcctgg cgaggcgccc aggacggagg ccgccctggc
     5041 cttcgtgtcg acgacgcgcc ggtcgacgga tgaggcgacc cagccggagg tgcccatgta
     5101 gacgtgggcc ctgcccaggc cgacggcgcc ggagccgagg gcgatcatgg agaggtcgcc
     5161 gccgccgccg aagacgggcg tgccagcctc gaggccgagc tcggcggcgg ccccgggcag
     5221 gagggggccg gccgcctccg aggcgccgat gacctccggg aggtggcgcg agtcgacgtc
     5281 gaagagccgg gcgaggaggg ggctccaccc gagcctcccc ggcctcgtgt cgtagaggaa
     5341 ggtcgcgttg gccgaatccg gcgtcatcgc gcagcggccg gtcgcgcggg ccacgaggta
     5401 ctccttcacg tcgagccact tcgccgcgcg ggcgaaggcc tcgggctcgt gggcccggac
     5461 ccagtggtac ttccagacgg ggtccttcgc gctcgcggag acccctcccg cgaggtagag
     5521 ggaggggaga aggcgcgaca ggcccatgcc ctcgaccttg aggccccgct ccaggccgcg
     5581 ccgcttctcc tcccgcgccc gctggtccat gtagctcatc gcgggcctta cgggcctgcc
     5641 ccccgagtcc acgaggacga ggccctgcat ctggcagcag aaggccatgc cgcggaccgc
     5701 gctcgggggg aggcccgcgg ccgcgagggc cgcgcgggcg ccgccggccg ccgctctcca
     5761 ccagtcctcg ggatcctgct cggccccgcc gtcggggagg atgcggatcg cgtactccgc
     5821 cccggcgcag gcgacgagct caagggagcc gcccgcctcg ggaaggcggt agaggcagga
     5881 tttcgcgctc gtggtcccga cgtcgacggc caggacgtgg gcgtcgctca tcgctcgccc
     5941 cgcccgcccg tccgggactc gccgcccggg acgccgccgg cggcggcgcc gagcttcgag
     6001 tacttgcgca ggtagacctc gtgcatggcc caggcctcca ccttggcgag gggcttcgcg
     6061 ccgcccagga actcggcctc gatgaagacg cggcagccct tctccgccat gcgcgcgcag
     6121 aggagggcct cggccatgct ccgccccagg gcgaccgcgc cgtggttggc gaggagggcg
     6181 gccatgcggc cgcggagcgc cttgacgacg acgcgggcca tccggcccga gccgggcagg
     6241 gcgtagtcgg cgacgcggag gctcggcccc acgacctggg cgaagtcgtc caggacgggc
     6301 ggcacctccc tcctcgcggc cgcgacggtc gaggcgctcg ccgagtgggt gtggacgatc
     6361 gccccgacct cgggccgcgc ggcgtagatc gcgcggtgga ggggcgtctc cgtggagggc
     6421 ttcccgccgc tcgcctcgcc cgtcgcgagg tccacgagga ctatgtcccc ggggcccagg
     6481 ctcctgtagt cgcgccccga gggcgtgatg gccatgaggc tccggccgtc cggccccttg
     6541 aagcggatcg agaggttccc ccaggtgccg tccacgagcc ccgtgtcgac gagctcgacc
     6601 ccggcccgcc tgaccgcttc cctcgcctcg ccctcgtcca tggccctcac tcccttcccg
     6661 cggcggcgcg cggggcctcg acgctctcga agacgcggcc gaagcgctcg accgcctcgt
     6721 ccaccacctc gtccgtgtcc gccgcgctcg tgtacatgcg gctccccgcg agggtgacga
     6781 ggccctccgc ggcgtaggcc gcgccgtact cctccatgag gcgcttccgc tccttgagct
     6841 cggcgagggt cttccggagg cgcaggtagt ccatcggcac gaacatcgcg ccgacggtct
     6901 ccaggtggca gatggagccc tggttgtagg cgacgaaggg gaggccgagc tcctcgatcc
     6961 tccgcctgag gccggccgtc agccggtcgc ccgcgcggcc cgcggcggcg caggcgtccc
     7021 gcttctcgat ctccctgagc gtgaagtagc ccgcggcggc ggagaggggg ttggcagcca
     7081 tcgtgcctcc gacgtaggcg cggcgcttgc cgctctccag gcccgccgcc atgaggagga
     7141 cgaggtcgcg ccggccgccc aggccgcccg cggaggggta gccgcccgcg acgatcttcc
     7201 cgaaggtcgt gaggtcgggc ctcacgccga agtagccctg ggcgccgccc aggcccacgc
     7261 ggaaggccgt cacgacctcg tcgaagacga ggagggcgcc gtacttgtcg cacaacgcgc
     7321 gcgcgcccga gttgtagtcc ctgtcgacgg gccgcgtgcc gctctccggc cccaggggct
     7381 cgaggaggac gcaggccgtg ccgccgcgga gccggttggc cctgaggacg cgctcgaggg
     7441 aggagaggtc gttgggagcc gcctcgtcgg tgagggagct catggcccgc gggatgccgt
     7501 gggcctcgta gcgccgggtc ttgggcacct tcaggctgta gacgagctgg tcgctccagc
     7561 cgtggtaggc gccgcccgcc ttcacgaccc tctgcttgcc cgtggccagg cgcgccaccc
     7621 ggatcgccgc catcaccgac tcggtgcccg agccgagcat gcggaacatc tcgaccgagg
     7681 gcatgtggcg gccgatctcc ttcgcgaggg cgagctcgaa ctcgtggaag aggccggtgg
     7741 aagggccgac tttttccagg acctcgagga cctggcgcct cacctcggga tcgttgctcc
     7801 cgaggatggt cgggccgccc gcctggagga agtcgaagta gcggcggccg tccacgtcgt
     7861 ggagccaagc tccctcggcg cggtcgatga cgagggggaa ggggtagttg aaggcgaggt
     7921 tgtgctggac gccgccgggg atgtagcgct tggcctccgc gacgatctcg cgggagcggg
     7981 gacattcgcg gtccaggcgc tctaggtagg cctccatcgc cgggcgggcg atggggcggg
     8041 ggggctccgc gaccaggcgg gccaggcggc cgtagatctc cgccgcgtcg gggtaggcgc
     8101 ttatggaata gttcatgttt gctcccttgg ctcaggttgg cgagtgagtg atcgctcacc
     8161 acaaacaagc
//
LOCUS       DAOOSJ010000112         8119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5566_length_8119_cov_6.069816, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000112 DAOOSJ010000000
VERSION     DAOOSJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5566_length_8119_cov_6.069816"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLG14_06835"
     CDS             complement(<1..686)
                     /locus_tag="PLG14_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48890.1"
                     /translation="MMTLRRRTLRFGLASSLALGAVLGLVWAMVAREGAARSLELEFQ
                     AYRASSALVEAYAQDPDFSLVKDSPVLGFGLYGSRGAAIQRRGGAPESLEPEAFALPS
                     FERSASGDSYVLIRPLGAAEAPWRGMMGPGMMGGQYGQGGMRGRGRLFSPPDPPAGGP
                     VPGWPLPPEPQAGAASGLAPGAASEPALMQGAAAPASAARAPRAIWLEYDQGGSDRER
                     RVLVAGAFLVS"
     gene            933..3197
                     /locus_tag="PLG14_06840"
     CDS             933..3197
                     /locus_tag="PLG14_06840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX48891.1"
                     /translation="MPATNARLALERAIKAGEDRDYEKAVELLQALVAETDAAPEAYL
                     YLGRSYHALGEWGKSIEALRLYVRAGGDRAAGFFFLGRAYLAAGRADEAVAFLKRSAE
                     ADSGRAQTWALLGAALLKERRSKAATGCLERAVGLAPGDRRIFHGYLNALFVRSARLL
                     SRGEADMARQMLSFAIANGLEGPGPRLWRARAFRELGRFPEAIADCEAALRQAPGDAS
                     LRWLRAGLLLSAGRGDEALDEFEALRADHPELPGIPRDEKALGRLRASIAFREERWSE
                     ALAESLAMLRAEPADPALRAMAAESLRALGQMERARDHWERAVEADPDEACFRLGLAL
                     VLWELGDYQGARATVERARRLGADPGEVEYYAALCDARLGAAPETLAPRIQALIRARG
                     ADPRLMFALGEVLYRSGRPDLAGGWFEKVLYLVPDHELSLLYRASVAESLGDEEALAA
                     AYEAYLEAYPDNDKLRREYVDRLFSGGRWQEAAAALEDGLPYGMGGERDRRRLALAYR
                     NAGRFREAAILYRDLLRASPSSPELLLSLALCLERDGKGDYALALLEKAPAAAKEGAA
                     PWIVLGLLYSRRGRTEAAVDSLRKATDIEPGNPRAWRDLGRIYERQGLSEFAARAYER
                     ADSLSPRTSARQTGGGDIPRPAKAPAARGKAAANKTAAAAAARKSAVSAKAATPAAAT
                     RKGAAGGKPAGAGKASRPAAAAGATKAAPESPAEASARKPRARPAAKPDSAAKPSGGR
                     TGGAAPGSRGKTRR"
     gene            3267..3827
                     /locus_tag="PLG14_06845"
     CDS             3267..3827
                     /locus_tag="PLG14_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48892.1"
                     /translation="MPVKAMDLFDAYAKSALPKDHGYLVSSFFSTHTAYSRYDVVSYA
                     NVKSIYPNEDGLTFQTDGKKLHILVEPPNYHQKGIEPYIRPSAEQIPHRFSELELYQC
                     KNQIKVYYGKDAVVSYGSFTVMRPTGMNFSFVFYALPDVYESMQMFFEKTFNKEAGVP
                     LADAKKVSAAVAAKVKTAMAYDFKAE"
     gene            complement(4013..6652)
                     /locus_tag="PLG14_06850"
     CDS             complement(4013..6652)
                     /locus_tag="PLG14_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOX48893.1"
                     /translation="MKEHIHFFSQNQTVKKGVDLEKLGQRGRQANEFAELALPILPGL
                     IIDADAAAALGKEKVAEALRPTLRKIEEIVGKRFGDAANPLLFKIVVSPNLAISNYPT
                     LHDFGLAKDTVDGFASWVGANFAAHEVLFLLRGALAVEEKVAEFEGRAKDLERIKAQA
                     AAVAKALASEKAPKGASALMDEYAQAVPAGFFSSAEAQLEIALKRISRLIELDEQDAE
                     DTAILVQPMVYGNYGKGSASGAFYTRDVVTGQARLQGEFYQERFNEIGAAGKDINAID
                     PAYLKELEKVSRKLEDKHKEIRQIRFTVENKKLWLIEQRQVVAKSTQADLRLLLDLKA
                     RKVVDAPFVVQSIAPGRLNEILHPVVNLASVKKLPGVTGGIAGAPGAAIGRVYFTTES
                     LLEAHRNAQQRGEDKRMILVMPSTFAEDVKAIEVAQGVLSCEGGYSAHASVVARQYGK
                     VSLVKPEMRIRGKKASIGELAVEEGDYITINVPYYGDPQVWAGKADLIEPDPKDSGLL
                     DFIALAKAETSRFHVRANADSPRDAALALMFGAEGIGLCRTEHMFFNEKRINVIREMI
                     LSDTREERVRALDKLKPMQRDDFYGLFKVMAGKEVTIRLLDAPLHEFLPHNPAEMDAL
                     IAYLGSRKGAKKLPKAEIQARCDSLSEFNPMLGHRGCRIAVSYPEIYEMQVRAIFEAV
                     YRLQKEKVDVRPEVMIPIVMNAAELKLIVFGKKIEGKTYRGLVDIEEEVRKELGTKAV
                     PFKIGTMIELPAAALGAGEIARYSEFFSFGTNDLTQTTLGISRDDFNSFMPDYSLYDL
                     IEGNPFSVLTPAVKDLVQIAVRRGKLSRPGLVAGLCGEHGANPDNIRFCMEVGLDYVS
                     CSPYQVPLSITAVAQAELDRKKA"
     gene            complement(6682..7737)
                     /locus_tag="PLG14_06855"
     CDS             complement(6682..7737)
                     /locus_tag="PLG14_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48894.1"
                     /translation="MKSLSCQFEGLADRPGFNASVRHIKTVIGAVERVEGKENLADVI
                     GELLEEKLLDKDEVLPVVSMVLGAKFKYRSVSLNLPSVAADLSAIPEESGKWNAVDLV
                     AVYHHPDLGPIVVNPKNPKSLARIDRLTRNELLVAWAGRFAKEGGEAASGDAARARAE
                     LFSGKKAKAKDSLLKGDCAYRAPREKAAPAAKAPRAAKAPRGPAAALQPKRGKAAQAA
                     PAPAAAPVPEHKPAVQAERAVKPGSRMTPMYSVQVTNELFHNGNVEAWKRIIASYNVK
                     YPDLQVLVYYDGERITDINALFKWGKVKHGSTIQFAVSGDEITGVAKLQRYLAQGASP
                     QFEAFLRGPVNAVLPLF"
     gene            complement(7901..>8119)
                     /locus_tag="PLG14_06860"
     CDS             complement(7901..>8119)
                     /locus_tag="PLG14_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48895.1"
                     /translation="EEPKRGTPGADYLSMLNAEPAALEESTMAPEPVNVRAPAKRGGA
                     KPKGVVDAGRKAPAKRGRRGEDSRMKEL"
BASE COUNT         1156 a         2832 c         2895 g         1236 t
ORIGIN      
        1 gatacgagaa aggcgcccgc cacgaggacg cgccgctccc tgtccgagcc cccctggtcg
       61 tactcgagcc agatcgcgcg tggcgccctg gccgccgagg cgggggctgc ggcgccttgc
      121 atgagggcgg gttcggaagc cgcgcccgga gcgaggccgg aggcggcccc ggcctggggc
      181 tccggcggga ggggccagcc agggaccggg cccccggcgg gcgggtccgg gggggagaag
      241 aggcggcctc gccccctcat cccgccctgg ccgtactggc cgcccatcat cccgggcccc
      301 atcatgccgc gccagggagc ctcggccgcg cccagggggc ggatgaggac ataggaatcg
      361 cccgaggcgc tccgctcgaa ggaaggcagg gcgaaggcct ctggctccag ggactcgggc
      421 gcgccgccgc gccgctgtat ggctgcgccc cgcgaaccgt agaggccaaa gccgaggacg
      481 ggagagtcct tgaccaggga gaaatccgga tcctgggcgt aggcttccac gagggccgag
      541 gaggcgcggt aggcctggaa ctcgagctcg aggctccgcg ccgcgccttc gcgggcgacc
      601 atggcccaca cgaggccgag gacggccccc agcgcgagcg aggaggccag gccgaatcta
      661 agcgtccgcc gccgcaaggt catcacgccc cgagtgtagc ttcaggcggc tcgccctggc
      721 aagcgcggct ttggcgggcg cgcccggcca tggctccgcg gcggccggcc ggcgcgcttc
      781 cacggcccgg ccgcctggcg cgtttccgcg gcggcccaga aaagcgcaac ggccgcatag
      841 gcggcgcaac cggtccgatt gcggcggcgg ggcgcttggg cggcgccgcg gggccgattg
      901 cggcggcccc tcccgcggtg tatccttggc ccatgcctgc gaccaacgcg cggctcgcgc
      961 tagagcgggc catcaaggcc ggagaggacc gtgactacga gaaggcggtc gagctccttc
     1021 aggccctcgt cgccgagacc gacgccgcgc cggaggccta cctctacctc ggccggagct
     1081 accacgccct gggcgagtgg ggcaagtcca tcgaggcgct caggctctac gtgcgagccg
     1141 gcggcgacag ggccgcgggc ttcttcttcc tggggcgggc ctacctcgcc gcgggccggg
     1201 ccgacgaggc cgtggccttc ctgaagcgga gcgccgaggc cgactcgggc cgcgcccaga
     1261 cttgggccct cctgggcgcc gccctcctca aggagaggcg gagcaaggcg gcgacgggct
     1321 gcctggagcg ggccgtgggc ctagcccccg gcgaccgccg gatcttccac ggctacctga
     1381 acgccctctt cgtccgctcc gcccgcctcc tttcgcgcgg cgaggccgac atggcccgcc
     1441 agatgctctc cttcgccatc gcgaacggac tcgaagggcc cggcccgagg ctctggcgag
     1501 ccagggcctt ccgcgagctc ggccgctttc ccgaggcgat agccgactgc gaggcggccc
     1561 tgcggcaggc tccaggggac gcctccctgc gctggctcag ggccggcctc ctcctctccg
     1621 ccggccgcgg cgacgaagcc ctggacgaat tcgaggccct ccgcgccgac catcccgagc
     1681 ttcccggcat tccccgggac gagaaggccc tgggccgcct ccgcgcctcg atcgccttcc
     1741 gggaggagcg ctggagcgag gccctggccg agtccctggc catgctccgc gccgagcccg
     1801 ccgatcccgc gctgcgcgcg atggccgcgg agagcctccg cgccctgggc cagatggaga
     1861 gggccaggga ccactgggag cgcgccgtgg aggccgaccc cgacgaggcc tgcttccgcc
     1921 tgggcctcgc ccttgtcctc tgggagctcg gcgactacca gggcgcccgc gccaccgtcg
     1981 agcgcgcgag gcgcctcggc gccgaccccg gggaagtcga gtactacgcc gccctctgcg
     2041 acgcccgcct gggcgccgcc cccgagaccc tggcgccccg catccaggcc ctcatcaggg
     2101 cgaggggcgc cgatccccgc ctcatgttcg ccctgggcga ggtcctctac cgctcgggcc
     2161 ggcccgacct ggccggcggc tggttcgaga aggtcctcta cctggtcccc gaccacgagc
     2221 tctccctcct ctaccgcgcc tccgtcgccg agtccctggg cgacgaagag gccctggcgg
     2281 cggcctacga ggcctacctc gaggcctatc ccgacaacga caagctccgc cgcgagtacg
     2341 tggaccgcct cttctcgggc ggccgctggc aggaggccgc agccgcgctc gaggacggcc
     2401 tgccctacgg gatgggcggg gagcgcgacc gccgcagact ggccctggcc taccgcaacg
     2461 ccggccgctt ccgggaggcg gcgatcctct accgcgatct tctccgcgcc tcgccctcga
     2521 gccccgagct cctcctctcc ctggcactct gcctggagcg cgacgggaag ggcgactacg
     2581 ccctggccct gctcgagaag gcgccggcag cggccaagga gggggcggcg ccctggatcg
     2641 tcctgggcct cctctattcg cggcgcggcc gcaccgaggc cgccgtcgac agcctgcgca
     2701 aggcgaccga tatcgagccc gggaacccgc gggcctggcg tgacctgggc aggatctacg
     2761 agaggcaagg cctttcggag ttcgcggccc gcgcctacga gcgcgccgac tccctgagcc
     2821 cgaggacttc ggccaggcag acaggcggcg gagacatccc tcgcccggcg aaggcgccgg
     2881 ccgcccgcgg caaggcagcc gcaaacaaaa ccgccgcggc cgccgccgcc cggaaaagcg
     2941 ccgtttcagc gaaagccgcc acgcccgccg ccgcaacccg gaaaggcgcg gccgggggaa
     3001 agcctgcggg cgccggcaaa gcctctcgcc cggccgccgc cgccggcgcg acaaaggccg
     3061 cgccggaatc ccccgcggag gcctcggcca ggaagccccg ggcgaggccg gccgcgaagc
     3121 cggattccgc ggccaagccc tcgggcggac ggaccggcgg ggccgcgccc ggctccaggg
     3181 gcaagactag acgttagccg gccataaacc gcgaggctta ttgcgttttt cttccctgag
     3241 gatatactcg tgagaggagg acactcatgc cggtaaaagc gatggacctg ttcgacgcct
     3301 acgcgaagag cgcgctgccc aaggatcacg gctacctcgt atcttcgttc ttcagcaccc
     3361 acacggccta cagccgctac gacgtggtgt cctacgccaa cgtcaagtcc atctacccca
     3421 acgaggacgg cctgaccttc cagaccgacg gcaagaagct ccacatcctg gtcgagccgc
     3481 ccaactacca ccagaagggc atcgagccct acatccgccc ctcggccgag cagatccccc
     3541 accgcttcag cgagctcgag ctctaccagt gcaagaacca gatcaaggtc tactacggca
     3601 aggacgcggt cgtctcctac ggctccttca ccgtcatgcg gcccacgggc atgaacttct
     3661 ccttcgtctt ctacgccctc cccgacgtct acgagtccat gcagatgttc ttcgagaaga
     3721 ccttcaacaa ggaggcgggc gtgcccctcg ccgacgccaa gaaggtctcc gccgccgtcg
     3781 ccgccaaggt caagacggcc atggcctacg acttcaaggc cgagtagcgg gacaagagtc
     3841 ccgcgccgcc gcgcaagccc gccccctcac ggggcgggtt tttctttggc ctccgcggaa
     3901 cggccgccgg tagggcataa aaacgccgcc cgggagactc ccgggcggcg aaggcgggca
     3961 ggtcgcgggg cgggcggcct ccggggtgcc cgccccgcga aaggaccggc ggctaggcct
     4021 tcttccggtc cagctcggcc tgggcgaccg cggtgatcga gagcggcacc tggtagggcg
     4081 agcaggacac gtagtcgagc cccacttcca tgcagaagcg gatgttgtcc ggattcgctc
     4141 cgtgctcgcc gcagaggccg gcgacgaggc ccgggcggga gagctttccg cgcctgaccg
     4201 cgatctgcac gaggtccttg acggccggcg tgaggacgga gaaggggttc ccctcgatga
     4261 ggtcgtagag ggagtagtcc ggcatgaagg agttgaagtc gtcgcggctg atgcccagcg
     4321 tcgtctgcgt gaggtcgttc gtcccgaagg agaagaactc gctgtagcgg gcgatctcgc
     4381 ccgcgcccag ggcggcggcc ggcagctcga tcatggtgcc gatcttgaag ggcacggcct
     4441 tggtgccgag ctccttcctg acctcctcct cgatgtcgac gaggccccgg taggtcttgc
     4501 cctcgatctt cttgccgaag acgatgagct tgagctcggc ggcgttcatc acgatgggga
     4561 tcatcacctc ggggcggacg tcgaccttct ccttctggag ccggtacacg gcctcgaaga
     4621 tcgccctgac ctgcatctcg tagatctcgg ggtaggagac cgcgatgcgg cagccgcggt
     4681 ggcccagcat cgggttgaac tccgacaggg agtcgcacct ggcctggatc tccgccttcg
     4741 gcagcttctt ggcgcccttc ctggagccca ggtaggcgat gagggcgtcc atctccgcgg
     4801 ggttgtgcgg gaggaactcg tggagcggcg cgtccaggag gcggatggtg acttccttgc
     4861 cggccatcac cttgaagagg ccgtagaagt cgtcccgctg catgggcttg agcttgtcca
     4921 gggccctcac ccgctcctcc ctcgtgtcgg agaggatcat ctcgcggatg acgttgatgc
     4981 gcttctcgtt gaagaacatg tgctcggtgc ggcagaggcc gatgccctcg gcgccgaaca
     5041 tgagggccag cgcggcgtcg cgcgggctgt ccgcgttggc gcgcacgtgg aaacgggagg
     5101 tctcggcctt ggccagggcg atgaagtcga ggaggcccga atccttgggg tcgggctcga
     5161 tgaggtcggc cttgccggcc cagacctggg gatcgccgta gtagggcacg ttaatggtga
     5221 tgtagtcgcc ctcctccacg gcgagctcgc cgatggaggc cttcttgccg cggatgcgca
     5281 tctcgggctt cacgagcgag accttgccgt actgcctcgc gacgacggag gcgtgggcgg
     5341 agtagccgcc ctcgcaggag aggacgccct gggcgacctc gatcgccttc acgtcctccg
     5401 cgaaggtgga gggcatgacg aggatcatgc gcttgtcctc gccccgctgc tgggcgttcc
     5461 ggtgcgcctc gaggaggctc tcggtcgtga agtagacgcg gccgatggcc gcgcccgggg
     5521 cgccggcgat gccgcccgtc acgccgggca gcttcttgac gctcgcgagg ttcacgacgg
     5581 gatggaggat ctcgttgagg cggccggggg ctatcgactg gaccacgaag ggcgcgtcga
     5641 cgaccttcct ggccttgagg tcgaggagga gccgcaggtc ggcctgggtg ctcttggcca
     5701 ccacctggcg ctgctcgatg agccagagct tcttgttctc caccgtgaag cggatctggc
     5761 ggatctcctt gtgcttgtcc tccagcttgc gggagacctt ctcgagctcc ttgaggtagg
     5821 cggggtcgat cgcgttgatg tccttgccgg ccgcgccgat ctcgttgaag cgctcctggt
     5881 agaactcgcc ctgcaggcgg gcctggcccg tcacgacgtc gcgggtgtag aaggcgccgg
     5941 aggcggagcc cttgccgtag ttgccgtaga ccatgggctg gaccaggatg gccgtgtcct
     6001 cggcgtcctg ctcgtcgagc tcgatgaggc gcgagatgcg cttcagggcg atctccagct
     6061 gggcctccgc ggaggagaag aagccggcgg ggacggcctg cgcgtactcg tccatgaggg
     6121 cggaggcgcc cttgggcgcc ttctcggagg cgagggcctt cgcgacggcc gcggcctggg
     6181 ccttgatgcg ctccaggtcc ttggcccgcc cctcgaactc ggccaccttc tcctccacgg
     6241 ccagggcgcc gcggaggagg aagaggacct cgtgggcggc gaagttggcg ccgacccagg
     6301 aggcgaagcc gtccaccgtg tccttggcga ggccgaagtc gtgcagggtc gggtagttgg
     6361 agatggcgag gttcggggac acgacgatct tgaagaggag ggggttggcg gcgtcgccga
     6421 agcgcttgcc gacgatctcc tcgatcttcc gcaaggtcgg cctgagggcc tccgcgacct
     6481 tctccttccc gagggccgcg gccgcgtccg cgtcgatgat gaggcccggc aggatcggca
     6541 gggcgagctc ggcgaattcg ttggcctggc ggccgcgctg cccgagcttc tcgaggtcga
     6601 cgcccttctt gacggtctgg ttctggctga agaagtggat gtgttccttc atggttcttc
     6661 cctttcccta tccgtccgcg cctagaacag cgggaggacg gcgttgacgg ggccccgaag
     6721 gaaggcctcg aactgcggac tcgcgccctg cgcgaggtac ctctggagct tcgccacgcc
     6781 cgtgatctcg tcgccggaga cggcgaactg gatcgtcgag ccgtgcttga ccttgcccca
     6841 cttgaagagg gcgttgatgt cggtgatgcg ctcgccgtcg tagtagacga ggacctggag
     6901 gtccgggtac ttcacgttgt agctcgcgat gatgcgcttc caggcctcca cgttgccgtt
     6961 gtggaagagc tcgttcgtga cctggacgga gtacatgggc gtcatccggg agcccggctt
     7021 cacggcgcgc tcggcctgga cggcgggctt gtgctcgggg acgggggccg cggccggggc
     7081 gggagccgcc tgggcggcct tgccgcgctt cggctggagg gcggcggccg ggccgcgggg
     7141 cgccttggcc gcgcgcgggg ccttggccgc cggggcggcc ttctccctcg gggccctgta
     7201 ggcgcagtct cccttgagga gggagtcctt ggccttggcc ttcttcccgg agaagagctc
     7261 ggcgcgggcg cgcgcggcgt cgccggaggc ggcctcgccg ccctccttgg cgaagcggcc
     7321 ggcccaggcc acgaggagct cgttccgcgt gaggcggtcg atcctggcaa ggctcttcgg
     7381 gttcttgggg ttgacgacga tgggcccaag gtcgggatgg tggtagacgg cgacgaggtc
     7441 gacggcgttc cacttccccg actcctcggg gatggcggag aggtccgcgg cgacggaggg
     7501 caggttgagc gagaccgacc ggtacttgaa cttcgcgccg aggaccatcg acacgacggg
     7561 caggacctcg tccttgtcga ggagcttctc ctcgaggagc tcgccgatca cgtcggccag
     7621 gttctccttg ccctcaaccc tctccacggc tccgatgacg gtcttgatat gccggaccga
     7681 ggcgttgaag cccgggcggt cggcgagtcc ctcgaactgg cagctgagac tcttcatgga
     7741 cgtaggcccc tccttttgac gaaagggccg cccgaaccgt ttcaagttcg gacggccctg
     7801 catgtatgat cttaacgcat cccgcgcgaa gcgcaaggag cttgcgcccc gaaagaggga
     7861 atcggcccgt ttcccggccg attccccagg gccgcgggga ttagagctcc ttcatcctcg
     7921 agtcctcgcc gcggcggccg cgcttggcgg gcgccttcct gccggcgtcg acgactccct
     7981 tgggcttcgc gccgccgcgc ttggcgggcg cgcggacgtt gaccggctcg ggggccatgg
     8041 tcgactcctc cagggcggcg ggctcggcgt tcagcatgct gaggtagtcg gcgccgggcg
     8101 tgccgcgctt cggctcctc
//
LOCUS       DAOOSJ010000113         8066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5606_length_8066_cov_7.268506, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000113 DAOOSJ010000000
VERSION     DAOOSJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5606_length_8066_cov_7.268506"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLG14_06865"
     CDS             <1..476
                     /locus_tag="PLG14_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48896.1"
                     /translation="ITALRREVLGFIFQSFNLVPVLTVRENVEFPLLLGGGRVGPSER
                     AWVDRLIEEVGLSDRREHKPGELSGGQRQRVAIARALVTKPKIVLADEPTANLDSKTG
                     EAIIRLMRLINRELGTTFVFSTHDAKIVAVADHVIRLEDGKVAENRLAAPAEASR"
     gene            473..1915
                     /locus_tag="PLG14_06870"
     CDS             473..1915
                     /locus_tag="PLG14_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX48897.1"
                     /translation="MNGAVLRMAFRNLKEHRRKSLTVGLIVAAASMLFVLGSSLLESA
                     TAGLRKSYRDRFTGDLYVTAKSPKKITAFGWDDPGSMGEPLPLLPRHDEVKAFAATIP
                     GVEALASRSVAFVQVSKEDSDAKGATLLMGVEPAEYARAFPEAFALAGGASFGPAGSG
                     LLASSFLSRGMGEAAGPGGAVLLSSVGMGSAIREERVLGVLPRDGANPFLNMTSYVGI
                     DSVRGLLGHYEGEGGAAEAAASAAAPAAALDEAELFGGEAVVEAEGAGIDAAALVASL
                     ASSGSPRTDASAWQSLVLKVRPEREVPAVREALESFLAREGIEAQVRGWEEGAGMLAS
                     SVLAMKRIFYYVCAVVAMVALLVIMNALVISVTERTGEIGTMRAIGASRGFVTRLIVS
                     ETLILSAASGLAGAAAGAGVVGLLSIRGIPAGNFLLRVMFGGPVLRPVLSAAAVGLCL
                     LAVLAIGAAASWYPTKVALRVNPATAMIRA"
     gene            1925..3277
                     /locus_tag="PLG14_06875"
     CDS             1925..3277
                     /locus_tag="PLG14_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX48898.1"
                     /translation="MKVLGIAMRNVRRQRRRALLLGGAVAVSFCAMTLMEGLTGGMAR
                     SLRANVEAGYVGELYVSGEEWREGGSPVKRLRSGGPALRTIEAAGLPVEAVVPKSGAE
                     AQIVYGGRSARASVAGISWEGGRSAIGSPALATGSLDAMAAEDGIVLPAAVAERLGVV
                     AGETVLVKASTVTGQANVAEFTVAATTAAKDGGELFQAAYARIEAVNELIGLPREGAQ
                     QLNVFLEGEPDLEAAAGRLLSALRSAGEAVVSREPKAAAGDGDDEDSGLPGGKGGPLA
                     ALFGGGESGEAWTGTRYSVATLDDYASQFLSILATIKAVSVVVFLALLSISAAGIVNT
                     FRMILIERTREIGAMRAMGMRARDVERLFVLEALVISVLGSLAGLAAAAATMGLASLP
                     RFGSGQFALFLSGGRLAFAASPASILAYLALVAGVSALAARGPARGAARLKPVDALRS
                     "
     gene            3293..4051
                     /locus_tag="PLG14_06880"
     CDS             3293..4051
                     /locus_tag="PLG14_06880"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX48899.1"
                     /translation="MKHLSAILLAALVAAAPAPAAAQEKPDFGKVLSLVDSRSSFPDA
                     DFSAVVRLVSEDPERGRSEDKVALFRRDSMDAFLMLALEPANKKGQGTLLVEDNLWFY
                     DPTSRKFTHTSLKDNYGESAAKNSDFRSSSKAEDYEVASYAEATLGKYECWVVDLKAR
                     RDDVTYPFMRLWVSKGEYLQLKAEEYSLGKRLLRTSLYPSYAKIGNKYVATRAIFQDA
                     LVAGKRTELLMGEISAKPLSDEMFTKGYLEKVGR"
     assembly_gap    4104..4203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4204..>5276
                     /locus_tag="PLG14_06885"
     CDS             <4204..>5276
                     /locus_tag="PLG14_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48900.1"
                     /translation="DARPEAGLRIHARTRYAVQDTDLSGELRLEELFADLSLEGYGRL
                     RAGKQAAAWGLGYWWSPADVLSLAPIDELDPEAEREGTLALKYHLPLGLGGLTLYAAA
                     DGASSLAETALAGRMEFVYGGAELGLGAFYRPDGEVAPRLLGTASFGLGPLDCYAEAV
                     LSLGSERKLASFEGAPSAASPEGALFSGALGAGYSGSDEEGRWDLSASAEYYLNGAGI
                     EEGGSYAENRAALLALVAGGELPAGGLRGYGRHYGALALRLDDLYGTGLGLGLDWLSN
                     LAEGSGYWRAAAAFEPEADFRAEAGFAAYYGPSGGEYSALGGRGSLVASLRLLERATL
                     SLDWPLYGEGAEPRASLVLEAAF"
     assembly_gap    5277..5376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5377..6094
                     /locus_tag="PLG14_06890"
     CDS             <5377..6094
                     /locus_tag="PLG14_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX48901.1"
                     /translation="GGAMRGRILVVEDVPEMAELVRDNLEFEGFEAELAGSAEAGLAA
                     LAAGRFDLVILDLNLPGMDGFEFLERLRREDATPVMIVSACGEDEDVVRGLGVGADEY
                     LQKPFSPKGLVARVRALVRRSREYGAAPRREAWRLGPYEIDLEARSVARGGERIALSS
                     REFEVLACLVRAGGKPLRSEELLSAAWDKAYGDLAAVGVYVQRLRRKLEEDPAAPRLL
                     VTERGFGYRLAGLAGGPGEA"
     gene            6097..7431
                     /locus_tag="PLG14_06895"
     CDS             6097..7431
                     /locus_tag="PLG14_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX48902.1"
                     /translation="MRLRTKLIGLAASVVFLPVLLVGLVLSSLGFTRIEALPIPRILV
                     AKEWFVALEGASGSLEELVAAGAKARGRIDGAIVGKGDELLLATRGGQPAWLEGRGVR
                     PGGLGPREMAWSRSLAVEGYGEVLALARVDLERDDPGINLPLLGGGLMFVLLVAGSAL
                     AAGSISRALRSLEASAARLRAGDLDSPVPPPRDPDAARVAEALESLRASLKEEEARRS
                     RLLMGVSHDFRTPIALIRGYAESLESGVARDEERRARYLRVIQDKTLELDGLVEELLD
                     YARMTTDQRRAAKAPADLRAFLAGLAAEFEEDATLGGRRFAFRDEAGAPVPCLLDARL
                     ARRAFENLFSNAMRYTEPGGLVELELELVAGDRAVASLSDDGIGVPEEDRERVFEPLY
                     RGSNVGNRRGSGLGLSVVKSVIEGLGWEIACAPRKGGGTRFSVVIPLGAGTA"
     gene            7561..>8066
                     /locus_tag="PLG14_06900"
     CDS             7561..>8066
                     /locus_tag="PLG14_06900"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX48903.1"
                     /translation="MAGESGYERKRREILEATLDILTSRGFEGTTLEAVADALGYTKQ
                     ALYYYFKSKEELLGSLVLGSLREGSAQVEAICAKEASAASRLKDLVRVFLDDHFLRHG
                     YFSITHMLKGFHEKMPEGPERSEVEALSARIPQAIIGMIGEGVAAGEFRNEDPKVLGG
                     IVFAMLSGV"
BASE COUNT         1018 a         2917 c         2766 g         1165 t
ORIGIN      
        1 cgatcacggc gctccgccgc gaggtcctcg gcttcatctt ccagtccttc aacctcgtgc
       61 ccgtcctcac ggtgcgcgag aacgtcgagt tcccgctgct gctgggaggc ggccgcgtag
      121 gcccctccga gcgggcctgg gtcgaccgcc tcatcgagga ggtaggcctc tccgaccggc
      181 gcgagcacaa gcccggcgag ctctccggag gccagcgcca gcgcgtcgcg atagcccgcg
      241 ccctcgtcac caagccgaag atcgtcctcg ccgacgagcc cacggccaac ctggactcca
      301 agacgggcga ggccatcatc cgcctcatgc ggctcatcaa ccgcgagctc ggcacgacct
      361 tcgtcttctc cacgcacgac gcgaagatcg tcgcggtcgc ggaccacgtg atccgcctgg
      421 aggacgggaa ggtcgccgag aaccgcctcg ccgcccccgc ggaggcctcg cgatgaacgg
      481 cgcagtcctc cgcatggcct tccgcaacct caaggagcac cggcggaagt ccctcacggt
      541 cggcctcatc gtcgcggcgg cgtccatgct cttcgtcctc ggctcgagcc tcctcgagtc
      601 ggcgacggcg ggcctccgga agtcctaccg cgaccgcttc acgggcgacc tctacgtgac
      661 cgcgaagtcg ccgaagaaga tcaccgcctt cggctgggac gaccccggct ccatgggaga
      721 gcccctgccc ctcctcccgc gccacgacga ggtcaaggcc ttcgcggcga cgatccccgg
      781 cgtcgaggcg ctcgcctcgc gctccgtcgc cttcgtccag gtctccaagg aagactccga
      841 cgcgaagggc gcgaccctcc tcatgggcgt ggagcccgcg gagtacgctc gtgccttccc
      901 ggaggccttc gccctcgctg ggggcgcgtc cttcggcccc gcgggctcgg ggctcctcgc
      961 ctcctccttc ctctcgcggg gcatgggcga ggccgccggg cccggaggag cggtcctcct
     1021 ctcctccgtc ggcatgggct ccgcgatccg cgaggagcgg gtcctcggcg tactcccccg
     1081 ggacggcgcg aatcccttcc tcaacatgac gagctatgtc ggcatcgact cggtccgcgg
     1141 gctgctgggc cactacgagg gtgagggcgg cgccgcggag gcggcggcgt ccgccgccgc
     1201 gccggccgcc gcgctcgacg aggccgagct tttcggcggc gaggccgtcg tggaggcgga
     1261 aggcgccggc atcgacgcgg cggccctcgt cgcctcgctc gcctcctccg gctccccgcg
     1321 gacggacgcc tcggcctggc agtccctcgt gctcaaggtt cgcccggagc gggaagtccc
     1381 cgcggtccgg gaggcgctcg agtccttcct cgcccgcgag gggatcgagg cgcaggtgcg
     1441 cggctgggag gagggcgcgg gcatgctcgc ctcgagcgtc ctcgcgatga agcggatctt
     1501 ctactatgtc tgcgcggtgg tggccatggt cgccctcctc gtcatcatga acgccctcgt
     1561 catctcggtc accgagcgga caggggagat cggcacgatg cgcgcgatcg gcgcgagccg
     1621 cggcttcgtg actcggctca tcgtctcgga gaccctcatc ctctccgcgg cctcgggcct
     1681 cgcgggagcg gcggcgggcg cgggcgtcgt cggactgctc tcgatccgcg gcatccccgc
     1741 gggcaacttc ctcctccgcg tgatgttcgg agggccggtc ttgaggcccg tcctctccgc
     1801 cgcggcggtc gggctctgcc tcctcgcggt cctcgcgata ggcgcggccg cgagctggta
     1861 cccgacgaag gtcgccctgc gggtgaatcc cgcgacggcc atgatccgag cctaggagcg
     1921 aagcatgaag gtcttgggaa tagcgatgcg aaacgtccgg cgccagcggc gccgggccct
     1981 tctcctcggc ggcgcggtcg ccgtgagctt ctgcgcgatg accctcatgg agggcctcac
     2041 ggggggcatg gcccggagcc tccgcgccaa cgtcgaggcg ggctacgtcg gggagctcta
     2101 cgtctccggc gaggaatggc gcgaaggcgg gagtcccgtg aagcggctca ggagcggcgg
     2161 tcccgcgctg cgcacgatcg aagccgcggg cctccccgtc gaggccgtcg tgccgaagtc
     2221 gggagccgag gcgcagatcg tgtacggcgg caggtcggcg cgagcaagcg tcgccggcat
     2281 ctcctgggag ggcgggcgct cggccatcgg ctcgccggcc ctcgccacgg gctccctcga
     2341 cgcgatggcg gcggaggacg gcatcgtcct ccccgccgcg gtcgcggagc gcctcggcgt
     2401 cgtcgccggc gagaccgtcc tcgtgaaggc ctccaccgtc accggccagg ccaacgtcgc
     2461 cgagttcacg gtcgccgcga ccacggcggc caaggacggg ggcgagctct tccaggcggc
     2521 ctacgccagg atcgaggcgg tgaacgagct catcggcctc ccgcgcgagg gcgcccagca
     2581 gctcaacgtc ttcctggagg gcgagcccga cctcgaggcc gccgcggggc ggctcctgtc
     2641 cgccctccgg tccgcgggcg aggccgtcgt ctcccgcgaa ccgaaggccg cggcagggga
     2701 cggagacgac gaggattcgg gcctgcccgg cggcaagggc ggccccctcg ccgcgctctt
     2761 cggaggcggc gaatcgggcg aggcctggac cgggacacgc tattcggtcg cgaccctcga
     2821 cgactacgcg agccagttcc tctcgatcct ggccacgatc aaggccgtct ccgtcgtggt
     2881 cttcctcgcc ctcctctcca tctccgcggc cgggatcgtc aacaccttcc gcatgatcct
     2941 catcgagcgg acgcgggaga tcggcgcgat gcgcgccatg ggtatgcggg cccgcgacgt
     3001 ggagcgcctc ttcgtcctcg aggccctcgt catctccgtc ctcggctccc tcgccggcct
     3061 cgccgccgcc gccgcgacga tgggcctcgc ctccctcccg cgcttcggga gcgggcagtt
     3121 cgccctcttc ctctcgggag gcaggctggc cttcgcggcc tcgcccgcct cgatcctcgc
     3181 ctacctggcc ctggtcgcgg gcgtctccgc cctggccgcc cgcggccccg cccgcggcgc
     3241 cgcgaggctc aagcccgtgg acgccctcag gtcctgacaa aggagcaccg taatgaaaca
     3301 cttatccgcc atcctcctcg cggccctcgt cgccgccgcc cccgctcccg ccgccgcgca
     3361 ggagaagccg gacttcggca aggtcctctc cctcgtcgac tcgcgctcga gctttcccga
     3421 cgccgacttc tccgcggtcg tccggctcgt gtcggaggat cccgagcggg gccggagcga
     3481 ggacaaggtc gcgctcttcc gccgcgactc gatggacgcc ttcctcatgc tcgccctcga
     3541 gcccgcgaac aagaagggcc agggcaccct cctcgtcgag gacaacctct ggttctacga
     3601 tccgacgagc cgcaagttca cccatacctc gctcaaggac aactacggcg agagcgccgc
     3661 gaagaactcc gacttccgga gctcctccaa ggcggaggac tacgaggtcg cctcctacgc
     3721 cgaggcgacg ctcggcaagt acgagtgctg ggtggtcgac ctcaaggcgc ggcgggacga
     3781 cgtgacctac cccttcatgc ggctctgggt atccaagggc gagtacctcc agctcaaagc
     3841 cgaggagtac agcctcggga agcggctcct ccgcacctcg ctctacccga gctacgcgaa
     3901 gatcgggaac aagtacgtcg cgacccgcgc gatcttccag gacgccctgg tcgcggggaa
     3961 gcgcacggag ctcctcatgg gcgagatctc ggcgaagccc ctttcggacg agatgttcac
     4021 gaagggctat ctcgagaagg tggggaggta ggcatggcgg cgcgcatcgc gcgccgcgga
     4081 cgcgccctcg cgtccgcggc cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnngacgccc ggcccgaggc ggggctcagg atccacgccc ggacgcgcta cgcggtccag
     4261 gacaccgacc tctcgggcga gctccgcctg gaggagctct tcgcggacct ctcgctcgag
     4321 ggctacggca ggctccgcgc gggcaagcag gcggccgcct ggggcttggg ctactggtgg
     4381 agccccgcgg acgtcctctc cctcgcgccc atcgacgagc tcgatcccga ggcagagcgc
     4441 gagggcaccc tcgccctcaa gtaccacctg ccgctcggcc tgggcggcct cacgctctac
     4501 gccgcggcgg acggcgcctc ttcgctcgcg gagacggccc tggccgggcg gatggagttc
     4561 gtctatggcg gcgccgagct cggcctcggc gccttctacc ggcccgacgg cgaggtggct
     4621 ccccgcctcc tcggcaccgc ctccttcggc ctcgggcccc tcgactgcta cgccgaggcc
     4681 gtcctctcct tgggcagcga gcggaagctc gcgagcttcg agggggcgcc ctcggccgcg
     4741 agcccggaag gggcgctctt ctccggcgcc ctcggcgcgg gctattcggg ctcggacgag
     4801 gaggggcgct gggatctctc ggcctccgcg gagtactacc tgaacggcgc ggggatagag
     4861 gagggcggct cctacgcgga gaaccgcgcc gccctcctcg ccctggtcgc gggcggcgag
     4921 ctgcccgcgg gcgggctccg cggctacggc cggcactacg gcgccctcgc gctccgcctg
     4981 gatgacctct acggcacggg cctcggactc ggcctcgact ggctctccaa cctcgccgag
     5041 ggctcgggct actggcgcgc cgcggcggcc ttcgagcccg aggcggactt ccgcgcggag
     5101 gcgggtttcg ccgcgtacta cgggccctcg ggcggcgagt attcggcctt gggcgggcgg
     5161 ggctccctcg tcgcctcgct caggctcctc gagcgcgcga cgctctccct cgactggccg
     5221 ctctacggcg agggcgccga gccccgggcg agcctcgtcc tcgaggcggc cttctannnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggga ggcgcgatgc gcggcaggat
     5401 tttggtggtg gaggacgtgc ccgagatggc ggagctcgtc agggacaacc tggagttcga
     5461 gggcttcgag gccgagcttg cgggctcggc cgaggcgggc ctggccgccc tcgccgcggg
     5521 gcgcttcgac ctcgtcatcc tcgaccttaa cctccctggc atggacggct tcgagttcct
     5581 ggagcggctc cgccgggagg atgcgactcc cgtgatgatc gtctccgcct gcggcgagga
     5641 cgaggacgtg gtccggggcc tcggcgtcgg cgcggacgag tacctccaga agcccttctc
     5701 tcccaagggc ctcgtcgcgc gggtccgggc cctggtgcgg cgctcccgcg agtacggcgc
     5761 ggccccgcgg cgcgaggcct ggcgcctcgg gccctacgag atcgacctcg aagcccgctc
     5821 ggtcgcgcga gggggcgaaa ggatagccct ctcgtcgcgg gagttcgagg tgctcgcctg
     5881 cctggtccgt gcgggcggga agccgctccg gagcgaagag ctcctctccg cggcctggga
     5941 caaggcctac ggcgacttgg ccgcggtcgg cgtctacgtc cagcgcctca ggcgcaagct
     6001 cgaggaggat cccgccgctc cgcgcctcct ggtcacggag cgcggcttcg gctatcgcct
     6061 cgccggcctc gcgggcgggc cgggggaggc ctaggcatgc ggctcaggac caagctcatc
     6121 gggctcgccg cgagcgtcgt cttccttccc gtcctcctcg tcggcctcgt cctctcctcc
     6181 ctcgggttca cgaggatcga ggctttgccg atcccgcgga tcctcgtcgc gaaggaatgg
     6241 ttcgtcgctc tcgaaggcgc ctcgggctcc ctcgaggagc tcgtcgcggc gggcgcgaag
     6301 gcgcgcgggc ggatcgacgg ggcgatcgtc ggcaagggcg acgagctcct cctcgcgacc
     6361 cggggcgggc agcccgcctg gctcgagggc cggggcgtcc gcccgggcgg gctcgggccc
     6421 agggagatgg cctggtcgcg ctccctcgcg gtggagggct acggcgaggt cctcgccctc
     6481 gcgcgcgtgg acctggagcg cgatgatccc ggaatcaacc tgcccctcct cgggggcggg
     6541 ctcatgttcg tcctccttgt cgcgggcagc gcgctggcgg cgggctccat ctcgcgcgcg
     6601 ctccgctcgc tggaggcctc cgcggcgcgg ctccgcgccg gggacctcga ctcgcccgtg
     6661 ccgccgcccc gggacccgga cgcggcgcgg gtggccgagg cgctcgagtc gctccgcgcc
     6721 tccctcaagg aggaggaggc gcggcgctcg cgcctcctca tgggcgtctc ccacgacttc
     6781 cggacgccca tagccctcat ccgcggctac gcggagagcc tcgagtccgg ggtggcgcgc
     6841 gacgaggagc ggcgggcgcg ctacctccgc gtcatccagg acaagaccct cgagctcgac
     6901 ggcctcgtgg aggagctcct ggactacgcg aggatgacga ccgaccagcg ccgcgccgcg
     6961 aaggcccccg ccgacctcag ggccttcctg gccggcctcg ccgccgagtt cgaggaggac
     7021 gctaccctag ggggacggcg cttcgccttc agggacgagg cgggcgcgcc cgtcccctgc
     7081 ctcctcgacg cccggctcgc gcgccgcgcc ttcgagaacc tcttctcgaa cgcgatgcgc
     7141 tacacggagc cgggcggcct ggtcgagctg gagctcgaac tcgtcgcggg cgaccgcgcg
     7201 gtcgcgagcc tctccgacga cggcatcggc gtgcccgagg aggaccgcga gcgcgtcttc
     7261 gagcccctct accggggctc caatgtcggg aaccgccgcg gctcgggcct gggcctctcc
     7321 gtcgtgaaga gcgtcatcga gggcctgggc tgggagatag cctgcgcgcc gcgcaagggc
     7381 ggcgggacgc gcttctccgt ggtcatcccc ctcggggcgg gaacggccta gcgcgcgggt
     7441 aggcggccgg ggctggcgaa aagcgaaaac cgacctcgct acaccttgac cttcctcgtc
     7501 cttcccgata ttttaactct cggtaaaaga ttttaccttt gggtaaaacc gggagggccg
     7561 atggcgggcg aatcgggcta cgagcggaaa cgcagggaga tcctcgaggc caccctcgac
     7621 atactcactt cccgcggctt cgagggcacg acgctggagg ccgtggcgga cgcgctcggc
     7681 tacaccaagc aggccctcta ctactatttc aagagcaagg aagagctcct cggctccctg
     7741 gtcctgggct cgctccggga aggctcggcc caggtggagg cgatctgcgc gaaggaggcc
     7801 tcggccgcct cgaggctcaa ggacctggtc cgcgtcttcc tcgacgacca cttcctccgc
     7861 cacggctact tctccatcac gcacatgctc aagggcttcc acgagaagat gcccgagggg
     7921 ccggagcggt ccgaggtcga ggcgctgtcg gcgcgcattc cccaggccat catcgggatg
     7981 ataggggagg gcgtggcggc cggcgaattc aggaacgagg accccaaggt gctcggcggc
     8041 atcgtcttcg cgatgctgtc gggcgt
//
LOCUS       DAOOSJ010000114         8014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5648_length_8014_cov_5.298907, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000114 DAOOSJ010000000
VERSION     DAOOSJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_8014_cov_5.298907"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLG14_06905"
     CDS             complement(<1..1097)
                     /locus_tag="PLG14_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GCN5 family acetyltransferase"
                     /protein_id="HOX48904.1"
                     /translation="MPVEIRRVEGDRGLKAFIQFQYDLYKGNAYAVPPLYFDELNTLS
                     KKRNPAFEFCEAEYWMAYKDGKAVGRVAGIYNPRYVEKWGLKNARFGWIDFIDDPEVS
                     EALLRTVEDWARSKGMEGIVGPMGFTDADKEGMLVEGFEELGTMPMIYNHPYYREHLE
                     RLGYAKDVDWLEFEVKVPEWIPEKALRVQELISKRSGIRLLDWKSKRQLKRDYAKPLF
                     HLLDEAYAGLYGTVPMTEKQVDVYIKQYLGFVDPRFTKLVVDEKGELIAFGIAMPSLS
                     RALQKGGGRLFPFGWLHLLRALAKPEGLDLYLIAVKQEYQSRGVIAFMMTSLTQDCIK
                     AGIRTAETAGELESNHAVQSLWKDFERRQHKR"
     gene            complement(1100..1387)
                     /locus_tag="PLG14_06910"
     CDS             complement(1100..1387)
                     /locus_tag="PLG14_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48905.1"
                     /translation="MDDRAGSAMRWREEKYDEVYESALRGLKRRREVDPSYGLADAEG
                     SLKHLYVQDGNDQGGRGELQDIILAATIAAHEHHIAEWKAEAAAAARREGE"
     gene            complement(1511..1583)
                     /locus_tag="PLG14_06915"
     tRNA            complement(1511..1583)
                     /locus_tag="PLG14_06915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1548..1550),aa:Met,seq:cat)
     gene            complement(1715..2821)
                     /locus_tag="PLG14_06920"
     CDS             complement(1715..2821)
                     /locus_tag="PLG14_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX48906.1"
                     /translation="MPQRKAFPDIAPSAWEHPADRAALAAFSSLPMAAEIVRRFSGST
                     FERSYRLAFLASSARVSEEQFPQVRRLVEEAARVLDVSPAPEAFVAFGLEPNAAAYGV
                     ERPFIVVSSAALDFWDEAELLAVLGHEMGHVASGHAAYKTLLALLLKAGSLLGGGLLG
                     GAALAAARAALLEWDRKSELSADRAGLLACQDPQASYRALMKTAGGPRQADMDLNEFF
                     RQARDYDASAEGLDSLWKLLDLLDENHPLAAPRMTALQDWEKGGGYEAVLRGEYPRRA
                     EGQGEGGPFRGFEAARDSYARDFSASQDPLSQAAGRVFEALGGIFGQGRGATQGQAGG
                     TGETGGPEGQGGQSGPEPGRRSVEELLDELFGKR"
     gene            3007..3396
                     /locus_tag="PLG14_06925"
     CDS             3007..3396
                     /locus_tag="PLG14_06925"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOX48907.1"
                     /translation="MQRKPLLERLKARAESLRRETYALYLAFREPGVPWYAKAFTALV
                     VGYALSPVDLIPDFIPVLGYLDDLVLIPAGIALARKLIPAQVLADCRARAERELAGKK
                     LTSRWAGAVIILVWTGVLALAVKAIFF"
     gene            3456..4511
                     /locus_tag="PLG14_06930"
     CDS             3456..4511
                     /locus_tag="PLG14_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOX48908.1"
                     /translation="MNYAQFSEALFAQIDAAPEIPLEHDARVVILSDLHAGDGSARDD
                     LSRNGALLLALLERHYLPRGSTLILNGDVEELQKYRLSTIRAAWPGLYGLFDRFSEAG
                     GLFKILGNHDEALELERSYPYPLYHGLSLRSGKDSLYVVHGHQASHFYANFNKLSGAL
                     VKYVVRTLGLPNLNVSKNSRRKFKIERRFYRFCQENKLVAVIGHTHRPLFESLSKYDA
                     LRFAIERLCREYPLADEVSRLEIAAALADYKEEFGRLRRRERRGDRGLGLYGRELLLP
                     CLFNSGCAVGKKGITALEIEDGKIELVYWYEVGKMRRYLERESARAFPLDGTPYRRVV
                     INSDRLDYLFARIGLLA"
     gene            complement(4553..5710)
                     /locus_tag="PLG14_06935"
     CDS             complement(4553..5710)
                     /locus_tag="PLG14_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX48909.1"
                     /translation="MHRVHSRRGPRPGSPTAPYRRARRDRWRDYFLAAPTPMWEEDFS
                     LVKERLDGLAAAGVEDFEAYFGSRPEELRSFADSVRVLEANDAVVDLLRAPDRSVLLG
                     GLSIGFDEESYSTFCEELSRIARGERRFELEIDVVDFAGKRLHLLLQWMAAPGSEERL
                     DRVYVSAVDITARAEAEEELRRSLAEKDALLREIHHRVRNNLAIVGSLLGLQSGCSDG
                     KVAEALAQAEKRIDAMAIVHDALYLSEDFARVSLEDCVAAVARGAFQREGLPASLRLE
                     TCVDDSTLPIEKAVACGILVHELVSNALEHAFPQGRGGRVRLEAKASPDGTIGLLVAD
                     DGVGLPPGVAPQTLDSLGFTLVRLLAGQLGGSLAAVGGPDGGLGLSIRFPA"
     gene            complement(5905..6324)
                     /locus_tag="PLG14_06940"
     CDS             complement(5905..6324)
                     /locus_tag="PLG14_06940"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOX48910.1"
                     /translation="MTRVLFLCVHNSARSQMAEAFLKRLGGGRFESESAGLEPGRLNP
                     YVVRAMAEAGVDISSNPTKSVFDLAAAGKTYDVVVTVCSKEAAERCPVFPGLAERLHW
                     PFADPSAFEGSDEEIMAKVRGVRDEIEKAVAELVAGR"
     gene            complement(6317..7411)
                     /gene="arsB"
                     /locus_tag="PLG14_06945"
     CDS             complement(6317..7411)
                     /gene="arsB"
                     /locus_tag="PLG14_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOX48911.1"
                     /translation="MSDTNKAGIGFFAKYLSLWVALCIALGVAIGYFLPGVPAFLGRF
                     EYAKVSIPVALLIWLMIYPMMLKVDFTSVRNAGKKPKGLVVTLVVNWLIKPFTMYLIA
                     WFFLKVAFKALIPGELSTEYLAGAVLLGAAPCTAMVFVWSYLADGDAGYTVVQVAVND
                     LVILFAFTPIVAFLLGVSDVRVPWDTLLLSVALFVVIPLGFGALTRSSLLRRRGKEWF
                     EGVFLKKFEGITEGGLLLTLVILFAFQGRVILANPLAIVLVAVPLVLQTYLVFFIGYG
                     WSKAWKVPYEVAAPAGMIGASNFFELAVAVAVSLFGLSSGAALATVVGVLVEVPVMLS
                     LVRIAKATRGSFSAAAPSKPQPGSSGAADD"
     gene            complement(7422..>8014)
                     /locus_tag="PLG14_06950"
     CDS             complement(7422..>8014)
                     /locus_tag="PLG14_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX48912.1"
                     /translation="MVVYMASMIGILVFVNWANSKGGSAVWDAIYSAKYWITGGFALV
                     LIYTIVRWFNKYERLEWVVATRDFSLQILPLLFGGVLVSGFLLGRPGHDALIPSAWIG
                     SLLGGNSLFANFLAALSGALMYFATLTEIPIVQGLLGAGMGQGPALALLLAGPSLSLP
                     SILVIAGELGWKKTLTYSGLVVALSTIAGAAFGAIA"
BASE COUNT         1286 a         2690 c         2847 g         1191 t
ORIGIN      
        1 cgcttgtgct ggcggcgctc gaagtccttc cagaggctct gcaccgcgtg gttcgactcg
       61 agctcgcccg cggtctccgc ggtcctgatc ccggccttga tgcagtcctg ggtgagggag
      121 gtcatcatga aggcgatgac cccgcgggac tggtactcct gcttgaccgc gatgaggtag
      181 aggtcgaggc cctcgggctt cgcgagggcg cgcaggaggt ggagccagcc gaaggggaag
      241 agccggccgc cgcccttctg gagggcgcgg gagaggctcg gcatcgcgat gccgaaggcg
      301 atgagctcgc ccttctcgtc cacgacgagc ttcgtgaagc gggggtcgac gaagcccagg
      361 tactgcttga tgtagacgtc gacctgcttc tccgtcatgg gcacggtgcc gtagaggccg
      421 gcgtaggcct cgtccaggag atggaagagg ggcttcgcgt agtcgcgctt gagctggcgc
      481 ttggacttcc agtcgaggag gcggatgccg gagcgcttgg agatgagctc ctggacgcgg
      541 agcgccttct cggggatcca ctcggggacc ttgacctcga actcgagcca gtccacgtcc
      601 ttcgcgtagc ccaggcgctc caggtgctcc cggtagtagg ggtggttgta gatcatcggc
      661 atcgtcccga gctcctcgaa gccctcgacg agcatgcctt ccttgtcggc gtccgtgaag
      721 cccatggggc ccacgatgcc ctccatgccc ttggacctgg cccagtcctc gaccgtgcgc
      781 agcagggcct cggagacctc ggggtcgtcg atgaagtcga tccagccgaa gcgggcgttc
      841 ttgaggcccc acttctccac gtagcgcggg ttgtagatgc cggccacgcg gccgaccgcc
      901 ttgccgtcct tgtaggccat ccagtactcg gcctcgcaga actcgaaagc cgggttcctc
      961 ttcttggaga gcgtgttgag ctcgtcgaag tagaggggcg gcacggcgta ggcgttgccc
     1021 ttgtagaggt cgtactggaa ctggatgaag gccttgaggc cgcggtcgcc ctcgacgcgg
     1081 cggatttcca ctggcatcgc tattcccctt cccggcgggc ggccgcggcc gcctcagcct
     1141 tccactcggc gatatggtgc tcgtgcgcgg cgatggtcgc ggccaggatg atgtcctgga
     1201 gctcgccgcg cccgccctgg tcgttgccgt cctggacgta gaggtgcttg agcgagcctt
     1261 ccgcgtcggc gaggccgtag gagggatcga cctccctccg ccgcttgagg ccccggagcg
     1321 cgctctcgta gacctcgtcg tatttctctt cgcgccagcg catggcgctt cctgctctgt
     1381 cgtccatggc gccgcggagt atactccggc ggcggcggcc ctgcaagccg cgttcccccg
     1441 ccgcggccgc cggctccgcc cccccgccag ggcgaaaaaa aaccggactc cgcgaaggag
     1501 tccggttcgg tagcgggagg ggggattgaa ccctcgaccc agcggatatg agccgcttgc
     1561 tctgccatct gagctatccc gccacgatcg ggtcaaaagg tatatacagg ctttgccctt
     1621 tttcgtcaag aggataggct cgggcggccc gcgatttgcg gcgggcggac gcgtgcggcc
     1681 ggcgggcgcg ttccgcccga gcccctgtcg ccgcctagcg cttcccgaag agctcgtcga
     1741 ggagctcttc cacggagcgc cgccccggct cggggccgga ctgcccgcct tggccctcgg
     1801 gaccgccggt ctccccggtc ccgccagcct gcccctgcgt ggctccgcgg ccctggccga
     1861 agatgccgcc cagggcctcg aagacgcggc ccgcggcctg cgagagggga tcctgcgaag
     1921 cggagaagtc gcgggcgtag gagtcgcggg cggcctcgaa gccgcggaag ggcccgccct
     1981 ccccttgccc ctccgcccgg cgaggatact cgccgcgcag gacggcctcg tagccgccgc
     2041 ccttctccca gtcctggagg gccgtcatgc gcggggcggc gaggggatgg ttctcgtcca
     2101 ggaggtcgag gagcttccag agggagtcga gcccctcggc cgaggcgtcg tagtcgcggg
     2161 cctggcggaa gaactcgttc aggtccatgt cggcctggcg ggggccgccg gccgtcttca
     2221 tgagggcgcg gtaggaggcc tgcgggtcct ggcaggcgag gaggccggcc cggtcggcgg
     2281 agagctcgct cttgcggtcc cattccagga gggccgcccg cgccgccgcg agggccgcgc
     2341 ccccgaggag gcccccccca aggagggagc ccgccttgag gaggagggcg aggagggtct
     2401 tgtaggccgc gtggcccgag gcgacgtggc ccatctcgtg gccgagcacg gccaggagct
     2461 ccgcctcgtc ccagaagtcc agggccgcgc tggagacgac gatgaagggg cgctcgacgc
     2521 cgtaggcggc ggcgttcggc tccaggccga aggcgacgaa ggcctcgggc gcgggcgaga
     2581 cgtcgaggac ccgcgccgcc tcctcgacga ggcggcgcac ctgcgggaac tgctcctcgg
     2641 agacgcgggc cgaggaggcc aggaaggcca ggcggtagga gcgctcgaag gtcgagcccg
     2701 agaagcggcg gacgatctcc gcggccatgg gcagggagga gaaggcggcg agggccgccc
     2761 ggtcggcggg gtgctcccag gccgagggcg cgatgtccgg aaaggctttt ctctgcggca
     2821 tgctccgact atagcgcccg ccccgcctgg agggaagcgc cgccgcgggg cgcctcgccg
     2881 gacccgcatg gagggtagcg ccgccgcggg gcgcaggcgg gcctccgcgg cgcgaggccg
     2941 ggcggcgagg cgaggcgcgc cgccctcccc ctcgaccctt ccgcggcgcc gctgtatcat
     3001 cccgccatgc agcgaaagcc cctcctggaa aggctcaagg cccgcgccga aagcttgagg
     3061 cgcgagacct acgccctcta tctcgccttc cgcgagccgg gcgtgccctg gtacgcgaag
     3121 gccttcacgg cccttgtcgt cggctacgcc ctctcccccg tcgacctcat tccggacttc
     3181 atcccggtcc tcggctacct ggacgacctc gtcctcatcc cggccggcat cgccctggcc
     3241 cggaagctca tccccgccca ggtgctcgcg gactgcaggg cgcgggccga gagggagctc
     3301 gccggaaaga agctcacgag ccgctgggcc ggcgccgtca tcatcctcgt ctggacgggc
     3361 gtcctggcct tggccgtgaa ggcgattttc ttctagcccg cgggctggcg aaggccgcgc
     3421 ccgaggcgtt aggcttgacg tagcgaggcc agcccgtgaa ctacgcccaa ttctccgagg
     3481 cccttttcgc gcaaatcgac gcggcgccgg agatacccct ggagcacgac gcgcgcgtcg
     3541 tcatactctc cgacctccac gcgggcgacg gctcggcccg cgacgacctg tcgcggaacg
     3601 gcgccctcct cctcgccctc ctggagcgcc actacctccc gcgcggctcg accctgatcc
     3661 tcaacggcga cgtcgaggag ctgcagaagt accgcctttc cacgatcagg gcggcctggc
     3721 caggcctcta cggcctcttc gaccgctttt cggaagccgg cggcctcttc aagatcctcg
     3781 ggaaccacga cgaggccctg gagctcgagc ggagctaccc ctaccccctc taccacggcc
     3841 tgtccctccg ctcggggaag gacagcctct acgtcgtcca cggccaccag gcctcgcatt
     3901 tctacgcgaa cttcaacaag ctctcgggcg ccctcgtgaa gtacgtcgtg cggaccctgg
     3961 gccttccgaa cctcaacgtc tccaagaatt cccgccgcaa gttcaagatc gagcggaggt
     4021 tctaccgctt ctgccaggag aacaagctcg tggccgtgat cggccacacg caccgccccc
     4081 ttttcgagtc cctgtccaag tacgacgccc tccgcttcgc catcgagcgc ctgtgcaggg
     4141 agtaccccct cgccgacgag gtctccaggc tcgagatcgc ggccgccctc gcggactaca
     4201 aggaggagtt cggccgtctc aggcgccgcg agaggcgcgg cgaccggggc ctgggcctct
     4261 acggaaggga gctcctcctc ccctgcctct tcaactcggg ctgcgccgtg ggcaagaagg
     4321 gcatcacggc cctggagatc gaggacggca agatcgagct cgtctactgg tacgaggtgg
     4381 ggaagatgcg ccgctacctg gagcgcgagt cggcgagggc cttccccctg gatggcacgc
     4441 cctaccgccg cgtcgtcatc aactccgacc gcctcgacta cctcttcgcg aggataggcc
     4501 tcctcgccta gccgcaggtg gccgggcggc cgccgcgagc ggccgccgcg gcctaggcgg
     4561 ggaagcggat cgagaggccc aggccgccgt ccggcccccc gaccgccgcg aggctcccgc
     4621 ccagctggcc ggcgaggagg cggaccaggg tgaagccgag gctgtccagg gtctgcggcg
     4681 cgacgcccgg aggcaggccc acgccgtcgt cggcgacgag gaggccgatc gtgccgtccg
     4741 gcgaggcctt ggcctcgagc cgcacgcggc cgccgcgacc ctgggggaag gcgtgctcga
     4801 gggcgttcga cacgagttcg tggacgagga tgccgcaggc gacggccttc tcaatcggaa
     4861 gggtcgagtc gtcgacgcag gtctccaggc ggagcgaggc cgggaggccc tcgcgctgga
     4921 aggcgccgcg ggccacggcg gccacgcagt cctccaggct cacccgcgcg aagtcctcgg
     4981 agaggtagag ggcgtcgtgc acgatggcca tcgcgtcgat gcgcttctcg gcctgggcca
     5041 gggcctccgc gaccttcccg tcggagcagc ccgactggag gccgaggagg gagccgacga
     5101 tcgcgaggtt gttgcgcacg cggtggtgga tctcgcggag gagggcgtcc ttctccgcga
     5161 gggatcgcct gagctcctcc tccgcctcgg cgcgggccgt gatgtccacg gcggagacgt
     5221 agacgcggtc cagcctctcc tcgctgccgg gggcggccat ccactggagg aggaggtgaa
     5281 ggcgcttccc cgcgaaatcg acgacgtcga tctccagctc gaagcggcgc tcgccccggg
     5341 ctatccgcga aagctcctcg cagaaggtcg agtaggactc ctcgtcgaag ccgatggaga
     5401 gccccccgag aaggaccgag cggtccggag cgcggaggag gtcgacgacg gcgtcgttgg
     5461 cctcgaggac gcggaccgag tcggcgaagg agcggagctc ctcggggcgg gagccgaagt
     5521 aggcttcgaa gtcctcgact cccgcggccg cgagcccgtc gagccgctcc ttcacgaggg
     5581 agaagtcctc ttcccacatg ggcgtgggcg cggcgaggaa atagtcccgc caacggtcgc
     5641 ggcgggcgcg gcgatacggc gctgtcgggg agcccggcct tgggccgcgg cgggagtgga
     5701 cgcgatgcat tggggcgatc cttggccagt gctggtggag gtactggacg attttactat
     5761 caatgcgtcg ggctgtaaac gcctttccgc gctggcaggc ttgagccccg ccacgggact
     5821 caagcccttc gtaccgtttc gtagaatcac gatcgatccg ggacggcgcg ccgaggcccg
     5881 tcccgcgccc ccgaggccgc cccctcaacg tcccgcgacg agctccgcga cagccttctc
     5941 gatctcgtcg cgcacgccgc gaaccttcgc catgatctcc tcgtcgctcc cctcgaaggc
     6001 ggagggatcg gcgaagggcc agtgaaggcg ctccgcgagg ccggggaaga cggggcagcg
     6061 ctcggcggcc tctttggagc agaccgtcac aacgacgtcg taggtcttcc ccgccgcggc
     6121 caggtcgaag acgctcttcg tcgggttgga ggagatgtcg acgccggcct cggccatcgc
     6181 gcggacgaca tagggattga gcctgcccgg ctcgaggccg gcgctctccg actcgaagcg
     6241 tccgcccccc agcctcttca ggaaggcctc cgccatctgg ctgcgggcgg agttgtggac
     6301 gcagagaaag aggaccctag tcatcggccg ctccggagga gcccggctgc ggcttggacg
     6361 gcgcggcggc ggagaagctt ccgcgggtcg ccttggcgat ccggacgagg ctcagcatca
     6421 ccggcacctc gacgaggaca ccgacgacgg tggcgagggc cgcgcccgag gagagcccga
     6481 agaggctcac cgcgacggcc acggcgagct cgaagaagtt cgaggcgccg atcatgccgg
     6541 ccggggcggc cacctcgtag ggaaccttcc aggccttgga ccagccgtag ccgatgaaga
     6601 agaccaggta ggtctggagg accaagggca ccgcgacaag gacgatggcg aggggattgg
     6661 cgaggatcac gcggccctgg aaggcgaaga ggatgacgag ggtgagaagg aggcctccct
     6721 cggtgatgcc ctcgaacttc ttcaggaata cgccctcgaa ccattccttg ccgcggcgcc
     6781 tcaagaggct cgagcgcgtg agcgcgccga agccgagggg gatgacgacg aagagcgcga
     6841 cggacaggag gagggtatcc cagggaacgc gcacgtcgct gacgccgagg aggaaggcga
     6901 cgatgggcgt gaaggcgaag aggatgacca ggtcgttgac cgccacctgg accaccgtgt
     6961 agcccgcgtc gccgtccgcg agataggacc agacgaagac catcgcggtg cagggggcgg
     7021 ccccgaggag gacggctccc gcgaggtatt cggtggagag ttcgccgggg atcagggcct
     7081 tgaacgccac tttcaggaag aaccaggcga tgaggtacat cgtgaagggc ttgatgagcc
     7141 aattcaccac gagcgtgacg acgaggccct tgggcttctt gccggcgttc ctgacgctcg
     7201 tgaagtcgac cttgagcatc atggggtaga tcatgagcca gatgagcagg gccacgggga
     7261 tcgatacctt ggcgtactcg aagcggccga ggaaggcggg cacgccgggc aggaagtagc
     7321 ctatggccac gccgagggcg atgcagagtg cgacccacaa cgacaggtac ttggcgaaaa
     7381 aaccgatgcc ggctttgttg gtatcgctca tggacgctcc tttaggcaat ggcaccgaag
     7441 gcggcgcctg caatggtcga cagcgcgacg acgagtcccg agtaggtcag ggtcttcttc
     7501 catccgagct cgcccgctat gaccagtatc gacgggaggg agaggctggg tcccgcgagc
     7561 aacagcgcca aggccggccc ctggcccatg cccgcgccga ggagtccctg cactatggga
     7621 atctcggtca gtgtcgcgaa gtacatgagg gcgccggaga gcgcggccag gaagttggcg
     7681 aagagcgagt tgcccccgag gaggcttcca atccaagcgc tggggatgag agcgtcatgg
     7741 cccgggcgtc cgagcaggaa accggagacg aggacgccgc cgaagaggag gggcaggatc
     7801 tgcaaggaga agtcgcgggt cgccacaacc cactcgaggc gctcgtactt gttgaaccag
     7861 cgtacgatcg tatagatgag gacgagggcg aagcctccgg tgatccagta cttcgccgag
     7921 tagatagcgt cccagacggc gcttcctccc ttggaattag cccagttcac gaagacgagg
     7981 atgccgatca tgctcgccat gtagacgacc atct
//
LOCUS       DAOOSJ010000115         7981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5677_length_7981_cov_5.480444, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000115 DAOOSJ010000000
VERSION     DAOOSJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_7981_cov_5.480444"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /gene="feoB"
                     /locus_tag="PLG14_06955"
     CDS             <1..1368
                     /gene="feoB"
                     /locus_tag="PLG14_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOX48913.1"
                     /translation="EVEKVLDEARLRLERHFGRDAEIVVSERRYASVHGAVAESTRLA
                     GESRFSLSEKADALLMSPLLGLPAFLLVMYGIFKLTFSLGAYPTAWLEALFSWLSGLA
                     GSALPEGLSKSLVVDGILGGVGGVLSFVPPIVILFLCLSFLEDTGYMARAAFITDRFL
                     HRFGLHGQSFLPLILGFGCSVPAIMSARTLKSRRDRVATVLAVPFMSCGAKLPVYVLL
                     AGAFFPRNPAAAIMGVYGAGVVLALLSSLLLKRTILRGEATPFVMELPPYRLPTLRGI
                     LAHVGEKTGSYLRKAGTVILAASVLIWAVTTFPLAPEGATPDEALAYSAAGRAGKAVE
                     PLLSPLGFDWKIGVAAVTGFAAKEVVVSTLGVLYRTDLDGSEAGPRGLAERLKEDSGM
                     TPLVAFVLMLFVLVMPPCLAALAAIKAELGWRWLAFEAAYLAALSWALAFLVYRGGLL
                     LGLGA"
     gene            1370..1639
                     /locus_tag="PLG14_06960"
     CDS             1370..1639
                     /locus_tag="PLG14_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48914.1"
                     /translation="MGAFDAVFVSAALGAASLFLGRSALRVLRSFRKGAAPACAACAS
                     EAGPAQGSAGGGSAGCGADPGAALPGCPGCASCKACPKRSACPGA"
     gene            complement(1751..3043)
                     /locus_tag="PLG14_06965"
     CDS             complement(1751..3043)
                     /locus_tag="PLG14_06965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX48915.1"
                     /translation="MEPGDLVESIPEELFRGRVPGRPRGEIAALIDALPIGLFWKDLR
                     SFYLGCNAAFARDAGIASPAEIVGLTDFDLAWSSIAEKVREDDRAVMESGIARLGYED
                     PVTLRDGRRVWHHKNKLPIRGPDGRVVGVLGTYEDVSERRLAEERLALAIEGSGVGLW
                     DWQVAAGKLEVNERWAGICGYRLEELEPLSIETWKRLCEPGDFERAQAALEAHFSGRS
                     PTYECEVRMRHKEGRWIWVLDRGKLFERDPEGRPLRMSGTHLDITERKRLEEELLLAA
                     TRDKLTGALNRRAGLEALARELKAAERRGSSLAVGFVDVDGLKEVNDSLGHAAGDLLL
                     ASAARVLSLSLRETDLLARLGGDEFLLILPDCGQGEAEAIWDRVERSAREPAEGPAVA
                     MSHGFAFRRGAEGLGLDALVAMADAKMYADKRSRKKGR"
     gene            complement(3176..4222)
                     /gene="mglC"
                     /locus_tag="PLG14_06970"
     CDS             complement(3176..4222)
                     /gene="mglC"
                     /locus_tag="PLG14_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HOX48916.1"
                     /translation="MPAIDAAAKGTSGAVKRYKEFASKNAIMLVLLALVLVIGIINSS
                     FFSLSNIRNILIISSVRVIIALGVGGILITGGTDLSAGRTVGLASVIAASLLQRPDYA
                     DRMYRTLPLLPVIVPIALAVLAGLVVGLLNGLVVAKLKVPPFITTLGTMVIVYGVASI
                     YVDRPPRGAQPIGGLRPDFTKIGTGYLGPNGDLSIPYLILVAAAIIVCYWIMLNKTRL
                     GKNIYAIGGNSRAATVSGVNVPKNLILVYAIAGCLYGLAGALLAARTGGATNNYGMSY
                     ELDAIAACVIGGVSTAGGIGKISGMITGVLIFEILNNGLVILGVNAYWQQVIKGIIIV
                     AAVAVDIRKYLAKR"
     gene            complement(4240..5754)
                     /locus_tag="PLG14_06975"
     CDS             complement(4240..5754)
                     /locus_tag="PLG14_06975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOX48917.1"
                     /translation="MGEREFVLEMQGISKSFPGVRALDDVTLKVRPGSVHALMGENGA
                     GKSTLMKCLFGIYKPDSGEIAIEGEKAQLQDSKQALDRGVSMIHQELNPVPYRSVMEN
                     LWLGRYPTKGLGPFRLVDHARMRTDTAELFAGLNMEIDPAVWVSELSVSKIQQMEIAK
                     AVSYKSKIIIMDEPTSSLTETEVAHLFGIIRRLKSEGVSIIYISHKMEEILAIADEVT
                     IMRDGRLVGTYPAGELTTDLIIKRMVGRDLSNRFPPRENVPGEVVLKVEGLTSAEHQS
                     FRDVSFELRRGEILGIGGLVGAKRTELVETLFGMRPVASGEVYLRGKRIPVDSPIRGK
                     RNKMALLTEERRTTGIFPMLSVRDNAVIANIDRYVLKTRLLDRKRSEEDVRKSIDALR
                     VKTPGASTLIQDLSGGNQQKVILARWLLTEPEILILDEPTRGIDVGAKYEIYSIIADL
                     AKRGKSIIMISSEMPELLGMSDRILVMCEGRLTGTLDGKEATQERVMELATQFM"
     gene            complement(5972..6973)
                     /gene="mglB"
                     /locus_tag="PLG14_06980"
     CDS             complement(5972..6973)
                     /gene="mglB"
                     /locus_tag="PLG14_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HOX48918.1"
                     /translation="MKKILLALLVAAVLFAGCSGTASSGKAKIGVTIYKFDDTFMSGV
                     RRAIEANAADKAELSIVDSQNQQPTQNDQVDTFLTQKVKALAINPVDRTAAGVIVDKA
                     KAKNVPVVFFNREPLAEDMAKSDKVFYVGAKAEQSGTMQGEILAEYWKANPAADKNKD
                     GKIQYIMLTGEPGHQDALLRTEFSIKAVEAAGIVVDKLAEDTAMWNRVNGQEKMAAFL
                     AKFGDKVEAVLCNNDDMALGAIEALKAAGYFDGKKYMPVVSVDATAPALDALEAGTLL
                     GTVLNDADNQGKATFDLAYALAKGEDVAKAGWEITDGKYVWVPYQKVTKDNYKNFKK"
     gene            7306..7899
                     /locus_tag="PLG14_06985"
     CDS             7306..7899
                     /locus_tag="PLG14_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX48919.1"
                     /translation="MAEGIARLFGSGCEVVVHSLEDISASVTKICNGHVTGRKPGSPI
                     TDLGLGVLRKSFESREDIVGPYFSRTDSGKLLKSTTILIRNDQAEPIGFLCVNFDLSV
                     PLSQLLGEFSPSAEPVEQGEHYAPDVQELVSRAVAEELEAAARKTGLHPSEKNRRIVL
                     ELERRGVFEVKGAVEHAARDLGVTKHTVYKYLRELRG"
BASE COUNT         1108 a         2672 c         2794 g         1407 t
ORIGIN      
        1 gaggtggaga aggtcctgga cgaggcgagg ctccggctgg agcggcattt cgggcgggac
       61 gccgagatcg tcgtctcgga gcggcgctac gcctctgtcc acggcgccgt cgccgagtcg
      121 acgcgccttg ccggtgagtc ccgcttctcc ctctcggaga aggcggacgc cctgctcatg
      181 agccccctcc tcggccttcc cgccttcctc ctcgtcatgt acgggatctt caagctcacc
      241 ttctccctgg gggcttaccc cacggcctgg ctcgaggccc tcttctcctg gctttcgggc
      301 ctggcgggct ccgccctgcc ggaaggccta tcgaagtccc tcgtggtcga cgggatcctg
      361 ggcggcgtgg gcggcgtcct ctccttcgtg ccgcccatcg tcatcctctt cctctgcctt
      421 tccttcctgg aagacacggg ctacatggcg cgggccgcct tcatcacgga ccgcttcctc
      481 caccgcttcg gcctccacgg ccagtccttc ctccccctca tcctgggctt cggctgctcc
      541 gtcccggcca tcatgtcggc ccgcaccctc aagagccgcc gcgaccgcgt cgccaccgtc
      601 ctggccgtgc ccttcatgag ctgcggggcc aagctccccg tctacgtcct cctcgcgggc
      661 gccttcttcc cgcggaaccc cgccgccgcg atcatgggcg tctatggcgc gggcgtcgtc
      721 ctcgccctcc tctcctcact tctcctcaag aggactatct tgcggggcga ggccacgccc
      781 ttcgtgatgg agctgccgcc ctaccgcctt ccgaccctgc gaggcatcct cgctcacgtg
      841 ggcgagaaga ccggctccta cctcaggaag gcgggcaccg tgatcctggc cgcctccgtc
      901 ctcatctggg ccgtcacgac ctttcccctg gcgcccgaag gcgcgacgcc cgacgaggcc
      961 ctggcctaca gcgcggccgg ccgcgcgggc aaggccgtcg aacccctcct ttcgcccctg
     1021 ggcttcgact ggaagatcgg cgtggccgcc gtgacgggct tcgccgcgaa ggaggtcgtc
     1081 gtctcgacct tgggcgtcct ctaccgcacc gacctcgacg gaagcgaggc cgggccccgc
     1141 ggcctcgcgg agcggctcaa ggaggactcg ggcatgacgc cccttgtcgc cttcgtcctt
     1201 atgctcttcg tcctcgtcat gccgccctgc ctggccgcgc tcgcggcgat caaggcggag
     1261 ctcggctgga ggtggctcgc cttcgaagcc gcctacctgg cggccctgtc ctgggccctc
     1321 gccttcctcg tctaccgcgg agggctactc ctcggcctcg gagcctagca tgggagcctt
     1381 cgacgcggtc ttcgtctcgg ccgcccttgg cgccgcctcc ctcttcctgg ggaggtcggc
     1441 cctgcgggtc ctgcgctcct tcaggaaggg ggcggcgccc gcctgcgccg cctgcgcctc
     1501 cgaggccggg ccggcgcaag gctcggccgg cggcggctcg gccggctgcg gcgcggaccc
     1561 aggcgccgct ctgcccggct gcccgggctg cgcgtcctgc aaggcctgcc cgaagcgctc
     1621 cgcctgtccc ggggcctagg gctcgcgggc gggccgggtt gtcggcctga cgagccagct
     1681 ttttccttgc gtgcccgggc ggccggcctg acgggcccgg gcttttgcca aacaggcccg
     1741 gacggccggc ttatcggccc ttcttcctcg accgcttgtc cgcgtacatc ttggcgtcgg
     1801 ccatggcgac cagggcgtcc aggcccaggc cctcggcgcc gcggcggaag gcgaagccgt
     1861 ggctcatcgc gaccgcgggg ccttcggccg gctcgcgggc gctgcgctcc acgcggtccc
     1921 agatggcctc ggcctcgccc tgtccgcagt cgggcaggat gaggaggaac tcgtccccgc
     1981 ccaggcgggc caacaggtcg gtctcgcgga ggctcaggga gaggacgcgg gcggccgagg
     2041 ccaggaggag gtccccggcg gcgtggccca gggagtcgtt cacctccttg aggccgtcca
     2101 cgtcgacgaa gccgactgcg agggaggagc cgcgcctctc ggcggccttg agctcgcggg
     2161 ccagggcctc gaggccggcc cgcctgttca gggctcccgt gagcttgtcg cgggtcgcgg
     2221 ccaggaggag ctcctcctcc aggcgcttcc gctccgtgat gtcgaggtgg gtgccggaca
     2281 tgcgcaaggg gcggccttcg ggatcgcgct cgaagagctt gccccggtcc aagacccaga
     2341 tccagcggcc ctccttgtgg cgcatccgca cctcgcactc gtaggtcggc gagcgtccgg
     2401 agaagtgcgc ctccagggcc gcctgcgcgc gctcgaaatc cccgggctcg cagaggcgct
     2461 tccaggtctc gatggagagg ggctcgagct cctcgaggcg gtagccgcag atgcccgccc
     2521 agcgctcgtt gacctcgagc ttgcccgcgg ccacctgcca gtcccagagt cccacccccg
     2581 agccctcgat cgccagggcc agccgctcct ccgcgaggcg gcgctcgctc acgtcctcgt
     2641 aggtcccgag gacgcccacg acccgcccgt ccgggccgcg gatcgggagc ttgttcttgt
     2701 ggtgccagac gcgtctaccg tcgcgcaggg tgacggggtc ctcgtagccg aggcgggcga
     2761 tcccgctctc catgacggcg cggtcgtcct ccctgacctt ctccgcgatg gagctccagg
     2821 cgaggtcgaa gtcggtcagc ccgacgatct ccgcggggga ggcgatcccc gcgtccctcg
     2881 cgaaggccgc gttgcagccg aggtagaagg agcgcaggtc cttccagaag aggccgatgg
     2941 gcagggcgtc gatcagggcg gcgatctccc cgcgcggcct gcccggtact ctcccgcgga
     3001 agagctcctc ggggatcgac tcgacgagat cgccaggttc catgctggat ctagcttcaa
     3061 ggatagagga gagggaggcc cggcgcaagg aaaagggccg cctgaggaat cgattccccg
     3121 gcggccccgc ggaccagaag acgccggcgc cccaaggggc gcgcggttcc cggctctagc
     3181 gcttcgcgag gtacttcctg atgtcgacgg cgacggccgc gacgatgatg atgcccttga
     3241 tgacctgctg ccagtaggcg ttgacgccca ggatgacgag gccgttgttg aggatctcga
     3301 agatgaggac gccggtgatc atgccggaga tcttgccgat gccgcccgcg gtggagacgc
     3361 cgccgatgac gcaggccgcg atcgcgtcca gctcgtagct catcccgtag ttgttcgtcg
     3421 cgccgcccgt gcgggccgcg aggagggctc ccgcgaggcc gtagaggcag cccgcgatcg
     3481 cgtagaccag gatgaggttc ttggggacgt tgacgccgga gacggtcgcg gcgcgggagt
     3541 tgccgccgat cgcgtagatg ttcttcccca gcctcgtctt gttgagcatg atccagtagc
     3601 agacgatgat cgccgccgcc accaggatga ggtaggggat ggagaggtcg ccgttcggcc
     3661 ccaggtagcc cgtgccgatc ttcgtgaagt cggggcggag gccgccgatg ggctgggcgc
     3721 cgcggggcgg ccggtcgacg tagatggagg cgacgccgta gacgatgacc atcgtgccca
     3781 gggtcgtgat gaagggcggc accttgagct tcgcgaccac caggccgttg aggagcccta
     3841 cgaccaggcc cgcgaggacg gccaaggcga tggggacgat gaccgggagg aggggcaggg
     3901 tccggtacat gcggtccgcg tagtcgggcc gctggaggag ggaggcggcg atgacggagg
     3961 cgaggccgac ggtgcggccc gcggagaggt cggtgccgcc ggtgatgagg atgccgccca
     4021 ctcccagggc gatgatgacg cggacggagg agatgatgag gatgttgcgg atgttcgaga
     4081 ggctgaagaa gctcgagttg atgattccga tgacgaggac cagggcgagg aggacgagca
     4141 tgatggcgtt cttgctcgcg aactccttgt agcgcttcac ggcgccgctc gtgcctttcg
     4201 cggctgcgtc tatggcgggc atggctttcc tccgattgct cacatgaact gggtcgcgag
     4261 ttccatgacc cgctcctggg tggcttcctt gccgtcgagg gtgccggtga ggcggccctc
     4321 gcacatgacg aggatacggt cggacatgcc caagagctcc ggcatctccg agctgatcat
     4381 gatgatgctc tttccgcgct tggcgaggtc cgcgatgatc gagtagatct cgtacttcgc
     4441 gcccacgtcg atgccgcggg tcggctcgtc gaggatgagg atctcgggct cggtgaggag
     4501 ccagcgggcc aggatgacct tctgctggtt gccgcccgag aggtcctgga tgagggtgga
     4561 ggcgccgggc gtcttcacgc gcagggcgtc gatgctcttc ctcacgtcct cctcgctccg
     4621 cttgcggtcg aggaggcgcg tcttcaggac gtagcggtcg atgttcgcga tcaccgcgtt
     4681 gtcgcgcacg gagagcatgg ggaagatgcc cgtcgtgcgg cgctcctccg tgaggagggc
     4741 catcttgttc cgcttgcccc tgatgggcga gtcgacgggg atcctctttc cgcggaggta
     4801 cacctctccc gaggcgaccg gccgcatgcc gaagagggtc tccacgagct ctgtgcgctt
     4861 cgcgccgacg aggccgccga tgcccaggat ctcgccccgc ctgagctcga aggagacgtc
     4921 gcggaaggac tggtgctcgg ccgacgtgag cccctcgacc ttgaggacca cctcgcccgg
     4981 gacgttctcg cgcgggggga agcggttcga caggtcgcgg ccgaccatgc gcttgatgat
     5041 gaggtcggtc gtgagctcgc ccgcggggta ggtgccgacg agcctgccgt cgcgcatgat
     5101 cgtcacctcg tccgcgatcg cgaggatctc ctccatcttg tgggagatgt agatgatgga
     5161 gacgccctcc gacttgaggc ggcggatgat gccgaagagg tgggcgacct cggtctccgt
     5221 gagggaggag gtgggctcgt ccatgatgat gatcttggac ttgtaggaga cggccttcgc
     5281 gatctccatc tgctggatct tggagacgga gagctcggag acccagacgg ccgggtcgat
     5341 ctccatgttg aggccggcga agagctcggc cgtgtccgtc cgcatcctgg cgtggtcgac
     5401 gaggcggaag gggccgaggc ccttggtcgg gtagcgcccg agccagaggt tctccatcac
     5461 gctgcggtag gggacggggt tgagctcctg gtggatcatc gagacgccgc ggtccagggc
     5521 ctgcttggag tcctggagct gggccttctc gccctcgatg gcgatctcgc ccgagtccgg
     5581 cttgtagatg ccgaagaggc acttcatcag cgtcgacttg ccggccccgt tctcgcccat
     5641 gagggcgtgg acgctgccgg gcctgacctt cagcgtcacg tcgtcgaggg ccctgacgcc
     5701 ggggaagctt ttcgagatgc cctgcatctc gagcacgaat tcgcgttcgc ccatatgcgg
     5761 cgcctccggt aatggtcttc cgaaacgaaa agcggggcgc ccgccctttc tagaggcgcg
     5821 cgtccccgcg ttccgcgcgg cccggtcccg ggccgcggat ccgcgctccc ctcccttccc
     5881 gcgcggggcg ggcggacagg gggcgcgggg ctcccggcgg gccccggacg ccccgcgagg
     5941 ggcggcccgt cggcccgtcc gggaaggcgc cttacttctt gaagttcttg tagttgtcct
     6001 tcgtgacctt ctggtagggg acccagacgt acttgccgtc ggtgatctcc cagccggcct
     6061 tggcgacgtc ctcgcccttg gcgagcgcgt aggcgaggtc gaaggtggcc ttgccctggt
     6121 tgtcggcgtc gttcaggacg gtgccgagga gggtgccggc ctcgagggcg tcgagggcgg
     6181 gggcggtggc gtcgacggag acgacgggca tgtacttctt gccgtcgaag tagccggcgg
     6241 ccttgagggc ctcgatggcg ccgagggcca tgtcgtcgtt gttgcagagc acggcctcga
     6301 ccttgtcgcc gaacttggcg aggaaggcgg ccatcttctc ctggccgttg acgcggttcc
     6361 acatggcggt gtcctcggcg agcttgtcga cgacgatgcc ggcggcctcg acggccttga
     6421 tggagaactc ggtgcggagg agggcgtcct ggtggccggg ctcgccggtc agcatgatgt
     6481 actggatctt gccgtccttg ttcttgtcgg cggcggggtt ggccttccag tactcggcga
     6541 ggatctcgcc ctgcatggtg ccggactgct cggccttggc gccgacgtag aagaccttgt
     6601 cggacttcgc catgtcctcg gcgaggggct cgcggttgaa gaagaccacg ggcacgttct
     6661 tggccttggc cttgtcgacg atgacgccgg cggcggtgcg gtcgacgggg ttgatggcga
     6721 gggccttcac cttctgggtg aggaaggtgt cgacctggtc gttctgggtg ggctgctggt
     6781 tctggctgtc cacgatggag agctcggcct tgtcggcggc gttggcctcg atggcccggc
     6841 ggacgccgga catgaaggtg tcgtcgaact tgtagatcgt gacgccgatc ttggccttgc
     6901 cgctggacgc ggtgcccgag cagccggcga agagcacggc ggcgacgaga agagcgagaa
     6961 gaattttctt catggtcatc cctccaaata cacttttcgc gcctaaaggc gtgatgcgcg
     7021 cgtttcgcgc aatgagacga gtttagcggt ttacggaaaa cgtgtccata tctcccgggc
     7081 cgtatttcct gggctttata aacgaatagc cggcgggggc gagccggcgg cttgccgggc
     7141 ggggcggctg ttcttatact cgatggacca tctgatatac cagtcgagag gcggatcccg
     7201 atgaacgagg ttatcgaaga cggcccgagg ggcggcggct gcgccatccc gctatcccgg
     7261 cctttgagcc cttcggacaa gctggtgctt ctccgctatg agacgatggc cgaaggaata
     7321 gcccggctct tcggctccgg ctgcgaggtc gtcgtgcact cgctcgagga tatctcggcc
     7381 tcggtcacca agatctgcaa cggacacgtg acaggccgga aacccggatc cccgatcacc
     7441 gacctgggcc tgggcgtcct gcgtaaatcc ttcgaatccc gcgaggacat cgtcggcccg
     7501 tacttctcca ggaccgattc cggtaagctg ctcaagtcga ccacgatcct catcaggaac
     7561 gaccaggccg agcccatcgg ctttctctgc gtgaacttcg acctttccgt cccactgtcc
     7621 cagctgctcg gggagttttc gccttcggcc gagcccgtcg agcagggcga gcattacgcc
     7681 cccgacgtgc aggagctcgt aagccgcgcc gtcgcggagg agctcgaagc ggcggcgaga
     7741 aagaccggcc tccatccctc ggagaagaac cgacgcatcg tcctcgagct cgagcgccgc
     7801 ggcgtcttcg aggtgaaggg cgcggtcgag cacgcggccc gggacctcgg ggtgaccaag
     7861 cacacggtct acaagtacct gcgcgaactc cgaggctgaa cgacccgtct ccatcccagt
     7921 tccacaccca cctctgtccg ggcccaggcc caggcccagg cccaggccca ggcccaggcc
     7981 c
//
LOCUS       DAOOSJ010000116         7956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5706_length_7956_cov_6.142767, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000116 DAOOSJ010000000
VERSION     DAOOSJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_7956_cov_6.142767"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1426
                     /gene="tyrS"
                     /locus_tag="PLG14_06990"
     CDS             191..1426
                     /gene="tyrS"
                     /locus_tag="PLG14_06990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOX48920.1"
                     /translation="MNAALETLKERGFLQQCTDLEGLSKLMDSGPVTFYVGCDPTGPS
                     LHIGHMVPFFALRHLHEAGHHGIALIGAGTARIGDPSGKSEMRKMLSYEQLDANADSI
                     RAQLDRFVGFDGVTAYTENNKDWLASLNYIDFLRDIGRHFSVNRMLSFEAYRMRMETG
                     LSFIEFNYQLLQSYDFLQLYKRRGCRLQIGGDDQWGNIVAGIELIRRVEGAECFGLTF
                     PLVTTADGKKMGKTEKGALFLDPAQTSPYDFFQYWRNVCDADVERFLLMFTFLPAAEC
                     RELGRAKDAGINKSKERLAWEITALIHGKEEADKALAAAKAAFGAASGGDVTQVPSKG
                     IARADLDKGVGVLDLFVYAGLAPSKSEARRLVQQGGAVVNERKIEDEKASVDSGWLDS
                     EGALMLRAGKKRFFRLVAE"
     gene            1636..1821
                     /locus_tag="PLG14_06995"
     CDS             1636..1821
                     /locus_tag="PLG14_06995"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HOX48921.1"
                     /translation="MDIASLSMHIAEDRLADQVGAKVLSMALKTQEGAGATLLTTLAS
                     TETASDPALGSRLDAWA"
     gene            2028..3011
                     /locus_tag="PLG14_07000"
     CDS             2028..3011
                     /locus_tag="PLG14_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX48922.1"
                     /translation="MLKLERVVKTYETVRAVDGLSFEARPGEIFGLIGPNGAGKSTTI
                     RMIMNVISPDSGSIRFDGEAFSEAMKRRIGYLPEERGLHKKSKVEEILVYLASLKGVD
                     RATARVQASAWLERFDLADWKGRKVEELSKGMAQKVQFIGSVIHDPDLVLFDEPFSGL
                     DPLSQDLFLKTVLELKGRGKTVIFSTHVMEHAERLCDRLLLVRKGRELVSGSVSAIKE
                     RHGKKAVQIEFDGDASFVESLPFVEAVRSFPRWIEVSLRGAAGPDELLSAVAGRLSVR
                     RFEVMAPSLHSIFVSLVGGGEGAVAEGTGSEDGADGGPAEEALVAREASRA"
     gene            3004..4269
                     /locus_tag="PLG14_07005"
     CDS             3004..4269
                     /locus_tag="PLG14_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48923.1"
                     /translation="MPKIVAILRHEFRLAAANKAYVVLTILGPFLILAITILPGLLSM
                     SPAASSGGAPVAVAVEAASGSGPGASALAALASAAEKLGRKLVKVEAGAEALAAAKAG
                     VSQGGYAALILLPSSWEDSGATVFTKTGTEAVLYGAVEAALGEAAREIRARRSGVDPA
                     LARELSRGVALSVVKLGKDAAEEESGDQEFMTTLFTVMSFVMLLYMTTLLYGQLIGRS
                     VVQEKTSKTVEIMLSSVSARELMFGKILGLGLAGLLQYGVWAGIAVLLSTFAAPALGL
                     SAPAAVSPANLGWLVLFFVLAFFLYASGYAAIGAAAEDEHDLGQLAWPLLVFLIVPLV
                     MISSFVMNPDSSLSVALSLFPMTSPIVMLVRILVSPPPAWQVALSLGVLLASIYAMAS
                     GAARIFRVGILMTGKRRKLKEILRWARAR"
     gene            4444..5751
                     /locus_tag="PLG14_07010"
     CDS             4444..5751
                     /locus_tag="PLG14_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX48924.1"
                     /translation="MDFFRSSAFAALLFGALSSVSLPVGALLGLWTRPSRTVISAVMS
                     FGAGSLIAAISFELVNPAVERREAGFLPLALGLLVGCLVFIALSRAADQGGAWARKRS
                     TLLSRLRTTKRREARGILEKLGKVDIFRSLPPDEVQAILPYVSSRLLPKDIPVFLQGS
                     VGDCLYLIDSGKVRIEYSGEGGGRSLVAELGEGQTFGEMALIWNAPRSADALPLVDTK
                     AFVIRKEDFDLLAAASPAVKAAVAELAAERGRTGKIPEAAVSAEDWAREASRHLDESS
                     YRPTETEIKVAASEGRAGAATAIWLGLLLDGIPESLVIGASMSGFDASPALIAGLFMA
                     NLPESLSSSTMMRESGAKTGSILWMWSSLVIAVGVGALLGNLVSGALPPLAHSLFEGL
                     AAGSMLAMTAQTMLPEAYERNIGMVGLFTVLGFIATVFFHTLG"
     gene            complement(5823..6491)
                     /locus_tag="PLG14_07015"
     CDS             complement(5823..6491)
                     /locus_tag="PLG14_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOX48925.1"
                     /translation="MPRPFSERRVGGEPAAALWKPAGIPARGLEIVVLGLDEFEALRL
                     ADYEGLYQEAAAMRMGVSRQTFGRIVESARKKVAAALALGHALRIEGGPAIRETQGET
                     TMKIAVPTRDGMVDAHFGHCAYFSVFAVEGGKIVAEEKLESPEECGCKSGISGELARK
                     GVTKLVAGNIGEGAVRVLSSFGIETLRGASGKARSAVEDYLAGKPFEGGANCHEHHEG
                     CEHH"
     gene            complement(6496..7437)
                     /locus_tag="PLG14_07020"
     CDS             complement(6496..7437)
                     /locus_tag="PLG14_07020"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOX48926.1"
                     /translation="MNEDKGAAPDAVANKVTYVIGVGSGKGGVGKSTSAVLLAQACAA
                     KGLKVGLLDADITGPSVPRLLGIESFRADSDGEKLLPVLAEEGFGVMSINLLVEDEDA
                     PVIWRGPLLSRAVEQFWTDTAWGELDVLVVDLPPGTGDVLITALTALPVTGVAFVATP
                     QDLVSMVVAKAVGMAHAAEAHVLGIVENMGTIVCPGCGKEHPLFAAGEAGKSGAHRRG
                     LPLLGRFPFRPEIAQRGVIRWAELPEGLKAEALAFADAALEAAKKADAVIRTAKANRT
                     ASGGEDGKCEGCGCTGDCSDGCSGSSKEAAGGCGCGH"
     gene            7520..7915
                     /locus_tag="PLG14_07025"
     CDS             7520..7915
                     /locus_tag="PLG14_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOX48927.1"
                     /translation="MIQAFTSHEPLDHLGLYGAFLERETDSSGTAVLHHGRVKRPGKQ
                     VPDFKAVELKTLGSGADEGLLAIARRARADFALNQVLLVHRLGRLAAGDTVLLAIVSG
                     ATRDRCFAACSWIVDEVKKEELIELVELP"
BASE COUNT         1173 a         2854 c         2641 g         1288 t
ORIGIN      
        1 ctccttggag agttttgctc aataatagcg cgatacttgg agcgccgcaa gagagatatg
       61 cagaataggg ccctggcagg gctcggaatc ggagcgggct ccggtccttg cgcggccttc
      121 ggccccttcg ctataatcct ggggcctggc cgccccgagc ggccggagca tcccgatccg
      181 gaggacgcat atgaacgccg cactcgagac gctgaaagaa cgcgggttcc tccagcagtg
      241 caccgacctg gaggggctct ccaagctcat ggattccggc ccggtgacct tctacgtggg
      301 atgcgatccg acgggcccct ccctgcacat cggccacatg gtgcccttct tcgccctgcg
      361 ccacctccac gaggccggcc accacggcat cgccctcatc ggcgccggca ccgcgcgcat
      421 cggggacccc tcggggaaga gcgagatgcg caagatgctg agctacgagc agctcgacgc
      481 caacgccgac tcgatccgag cccagctcga ccgtttcgtc ggcttcgacg gcgtgaccgc
      541 ctacaccgag aacaacaagg actggctcgc gagcctcaac tacatcgact tcctgcgcga
      601 catcggccgg cacttctccg tgaaccgcat gctctccttc gaggcctacc ggatgcggat
      661 ggagacgggc ctctccttca tcgagttcaa ctaccagctc ctccagagct acgacttcct
      721 ccagctctac aagcgccgcg gctgccgcct ccagatcggc ggcgacgacc agtggggcaa
      781 catcgtcgct ggcatcgagc tcatccgccg cgtcgagggc gcggagtgct tcggcctcac
      841 cttccccctc gtcacgaccg ccgacgggaa gaagatgggg aagacggaga agggcgccct
      901 cttcctggac cccgcccaga ccagccccta cgacttcttc cagtactggc gcaacgtctg
      961 cgacgccgac gtggagcgct tcctcctcat gttcaccttc ctccccgccg ccgagtgccg
     1021 cgagctcggc cgggccaagg acgcggggat caacaagtcg aaggagcgcc tggcctggga
     1081 gatcaccgcc ctcatccacg gcaaggagga ggcggacaag gccctggccg cggccaaggc
     1141 cgccttcggc gcggcctcgg gcggcgacgt cacccaggtg ccctccaagg gcatcgcgag
     1201 ggccgacctg gataagggcg tcggcgtcct cgacctcttc gtctacgcgg ggctggcccc
     1261 ctccaagagc gaggccaggc gcctcgtcca gcagggcggc gccgtcgtca acgagaggaa
     1321 gatcgaggac gagaaggcga gcgtcgactc cggctggctc gactcggagg gagccctcat
     1381 gctccgcgcc gggaagaagc gcttcttcag gctcgtcgcg gagtagggcg gggccggcgc
     1441 cggaggcgag tccggacccg cggccgcgcg gatccgggtc ctttcccgcc ggcgccgcag
     1501 ctcccgctgg ctgcgaggct cgcgccggcg cggaaagccc cccgcaggcg gcgccccacg
     1561 tcaggaatcg gttaatcccg acgatttcct tgtcccccgc ttgcttatcc gggcgggaag
     1621 tggtattgtt tctacatgga tatcgcatcg ctgtccatgc atatcgccga ggatcgttta
     1681 gccgaccaag tcggggccaa agttctttcc atggccctaa agactcagga aggcgcggga
     1741 gccacgctcc tgaccaccct tgcgagcacc gagacagcct ccgatccggc cctgggttcc
     1801 cgactggacg cctgggctta gcaccgaagt tgagagagaa aagccgccgg atgggcggct
     1861 ttttgcattt ctggggcctt ccgtctccgc cgaacccgct tccgcgccct cccctatgac
     1921 aatgcgcatg cccggggctc cgcggcgggg ccctccccgc gccatattga tccaggctcc
     1981 ttcgcgcgct acagttccgg taccgcatat cgcgaaagga gcgtgccgtg ctcaagcttg
     2041 agcgggtcgt gaagacctac gagacggtgc gggcggtgga cgggctgtcc ttcgaggccc
     2101 ggcccggcga gatcttcggc ctcatcggac cgaacggcgc cgggaagtcg accaccatcc
     2161 gcatgatcat gaatgtcatc tcccccgact cgggctccat ccgtttcgac ggggaggcct
     2221 tctccgaggc catgaagcgg aggatcggct acctgccgga ggagcggggc ctgcacaaga
     2281 agtccaaggt cgaggagatc ctggtctacc tcgcctccct caagggagtc gaccgggcga
     2341 cggcccgcgt ccaggcctcg gcctggctcg agcgcttcga cctcgcggac tggaagggac
     2401 gcaaggtcga ggagctctcc aagggcatgg cccagaaagt gcagttcatc ggctcggtca
     2461 tccacgaccc cgacctcgtg ctcttcgacg agcccttctc cgggctcgac cccctgtcgc
     2521 aggacctctt cctcaagacc gtgctcgagc tcaagggaag aggcaagacc gtgatcttct
     2581 ccacccacgt gatggagcac gcggagcggc tctgcgaccg cctcctcctc gtgcgcaagg
     2641 ggagggaact cgtctccggc tccgtgtccg cgatcaagga gcgccacggg aaaaaggccg
     2701 tccagatcga gttcgacggc gacgcctcct tcgtggagag cctgcccttc gtcgaggccg
     2761 tccgatcctt ccccaggtgg atcgaggtct ccctgcgggg cgcggccggt cccgacgagc
     2821 tcctgtcggc cgtcgcgggc cgcctctccg tgcggcgctt cgaggtcatg gccccgtcgc
     2881 tgcattccat cttcgtgagc ctggtcgggg gcggcgaagg cgccgtcgcc gaaggcaccg
     2941 gcagcgagga cggagccgac ggcgggcccg ccgaagaagc gcttgtcgcc agggaggcca
     3001 gccgtgccta agatcgtagc catcctccgc cacgagttca ggctcgccgc ggccaacaag
     3061 gcctacgtcg tcctcaccat cttggggccc ttcctcatcc tcgcgatcac gatccttccg
     3121 ggccttctct cgatgagccc agcggcctcc tcgggcggcg cgcccgtcgc cgtggcggtg
     3181 gaggcggcct ccggttccgg cccaggcgcc tcggccctcg ccgccctcgc gtcggcggcg
     3241 gaaaagctgg gcaggaagct ggtcaaggtc gaggcggggg ccgaggccct cgcggcggcc
     3301 aaggccgggg tctcgcaagg cggctacgcg gcgctcatcc tcctgccctc aagctgggag
     3361 gactccggcg cgacggtctt cacgaagacg ggcaccgagg ccgtcctcta cggcgcggtc
     3421 gaggccgcct tgggagaagc ggcccgggag atccgcgcca ggcggagcgg cgtcgatccc
     3481 gccctcgcca gggagctctc ccgcggcgtc gctctctccg tcgtgaagct gggaaaggac
     3541 gccgcggagg aggagagcgg cgaccaggag ttcatgacga ccctcttcac ggtgatgagc
     3601 ttcgtgatgc tcctctacat gacgaccctc ctctacggcc agctcatcgg ccgctcggtg
     3661 gtgcaggaga agacctccaa gaccgtggag atcatgctct ccagcgtctc ggcccgggag
     3721 ctcatgttcg ggaagatcct cggcctcggc ctcgccggcc tcctccagta cggggtctgg
     3781 gcgggcatag ccgtcctcct gtccacgttc gcggcgccgg ccctcggcct ctccgcgccc
     3841 gcggccgtct cccccgccaa cctgggctgg ctcgtcctct tcttcgttct cgccttcttc
     3901 ctctatgcct cgggctacgc cgccatcggc gccgcggccg aggacgagca cgacctgggc
     3961 cagctggcct ggcccctcct cgtcttcctc atcgtccccc tcgtgatgat ctcctccttc
     4021 gtcatgaatc ccgactcgag cctgtcggtg gcgctctccc tcttccccat gacgagcccc
     4081 atcgtgatgc tcgtccggat cctcgtctct ccgccgccgg cctggcaggt ggcgctcagc
     4141 ctgggcgtcc tcctcgcctc catctacgcg atggcctcgg gcgcggcgag gatcttcagg
     4201 gtgggcatcc tgatgacggg aaagcggcgc aagctcaaag agatcctgcg ctgggcgagg
     4261 gcgcgctagc ctaaagcggc gccgctccgc ttccgtgcgg cgccggtagt cggggcggcc
     4321 gcgccgcctg acggagaccg cggccgccgc ggccgcgggg ggcgccaggc gccgccttcc
     4381 gttggaaaga gcccggcccg ggtcctatac ttgaagccga tccccgtcgg gaagggaggc
     4441 ttcatggact tcttccgatc ctcggccttc gcggccctcc tcttcggcgc gctgagctcg
     4501 gtctcgctcc cggtcggggc cctcctcggg ctctggacca ggccctcgcg gacggtcatc
     4561 tccgcggtga tgtccttcgg agcgggatcg ctcatcgcgg ccatctcctt cgagctcgtc
     4621 aaccccgcgg tcgagcgcag ggaggccggc ttcctccccc tcgccctggg ccttctcgtc
     4681 ggctgcctcg tcttcatcgc cctgtcgcgg gccgcggacc agggaggcgc ctgggccagg
     4741 aagcgctcca ccctcctttc ccggctcagg acgacgaagc ggcgcgaggc ccgcggcatc
     4801 ctggagaagc tcggcaaggt cgacatcttc cgctccctgc cgcccgacga ggtccaggcc
     4861 atcctcccct acgtctcctc gcgcctcctc cccaaggaca ttcccgtctt cctccagggc
     4921 agcgtcgggg actgcctcta cctcatcgac tcggggaagg tgcgcatcga gtactcgggc
     4981 gaggggggag ggcggagcct cgtcgccgag ctcggcgagg gccagacctt cggggagatg
     5041 gccctcatct ggaacgcgcc gcggagcgcg gacgccctgc ccctcgtgga cacgaaggcc
     5101 ttcgtcatca ggaaggagga cttcgacctc ctcgcggcgg cgagccccgc cgtcaaggcg
     5161 gccgtcgcgg agctggcggc ggagcggggg cggaccggga agatcccgga ggcggcggtg
     5221 agcgccgagg actgggccag ggaggcctcc cgccacctgg acgagagctc ctaccggccg
     5281 acggagaccg agatcaaggt ggccgcgagc gaggggaggg ctggcgccgc gacggcgatc
     5341 tggctcggcc tcctcctgga cggcatcccc gagtccctcg tgatcggcgc gagcatgtcg
     5401 ggcttcgacg cgagtcccgc cctcatcgcg ggcctcttca tggcgaacct tcccgagtcc
     5461 ctctcgagct cgacgatgat gagggagtcg ggcgccaaga cgggtagcat cctctggatg
     5521 tggagctccc tcgtcatcgc ggtcggcgtc ggcgccctcc tcgggaacct cgtctccgga
     5581 gccctgccgc ccctggccca ttccctcttc gagggcctgg ccgcgggctc catgctcgcg
     5641 atgacggccc agaccatgct tcccgaggcc tacgagcgca acatcggcat ggtcggcctc
     5701 ttcacggtcc tgggcttcat cgcgaccgtc tttttccaca ccctggggta gaatgcgggg
     5761 gccccgggcg cgaatcgctt cgcgcccgag gcccccggac tcgccgccgc agcgggaggc
     5821 tcctagtggt gctcgcagcc ctcgtggtgc tcgtggcagt tggcgccgcc ctcgaagggc
     5881 ttgccggcca ggtagtcctc cacggcggag cgggccttgc cggaggctcc gcggagcgtc
     5941 tcgatcccga aggaggaaag gacgcgcacc gccccttcgc cgatgttgcc ggcgacgagc
     6001 ttggtgacgc ccttccgcgc gagctcgccc gagatgccgc tcttgcagcc gcactcctcg
     6061 ggggactcga gcttctcctc ggcgacgatc ttgccgccct cgacggcgaa gaccgagaaa
     6121 taggcgcaat ggccgaaatg cgcgtcgacc atgccgtcgc gcgtgggtac cgctatcttc
     6181 atcgttgtct ctccttgcgt ttccctgatc gcgggcccgc cctctatcct gagggcatgg
     6241 cctaaagcca gcgcggccgc gaccttcttc ctggccgact cgacgatcct cccgaaggtc
     6301 tgccgcgaca cgcccatgcg catcgcggcc gcctcctgat agaggccttc gtagtcggcg
     6361 agcctcaggg cctcgaactc gtcgagcccg aggacgacga tctcgaggcc gcgggccggg
     6421 attcccgcgg gtttccagag cgccgccgcg ggctcgccgc cgacgcggcg ctccgagaag
     6481 gggcggggca tgtccttagt gtccgcagcc gcagccgccc gcggcttcct tcgacgagcc
     6541 cgagcagccg tcggagcaat cccccgtgca gccgcagccc tcgcacttcc cgtcctcgcc
     6601 gccggaggcc gtgcggttcg ccttcgcggt ccggatgacg gcgtcggcct tcttcgcggc
     6661 ctcgagggcc gcgtcggcga aggccagggc ctcggccttg aggccctcgg gaagctcggc
     6721 ccagcggatc acgccgcgtt gggcgatctc gggcctgaag gggaagcggc cgaggagggg
     6781 gagaccgcgg cgatgggcgc cggactttcc ggcctcgccc gcggcgaaga ggggatgctc
     6841 cttgccgcag cccgggcaga cgatggtgcc catgttctcg acgatgccca ggacgtgggc
     6901 ctcggcggcg tgggccatgc cgacggcctt cgcgacgacc atggagacca ggtcctgggg
     6961 cgtggccacg aaggcgacgc cggtgacggg cagggcggtg agggcggtga tgaggacgtc
     7021 gcccgtcccc ggaggcaggt caacgaccag gacgtcgagc tcgccccagg ccgtgtcggt
     7081 ccagaactgc tcgacggcgc gggagaggag gggaccccgc cagatcaccg gcgcgtcctc
     7141 gtcctcgacc aggaggttga tggacatgac gccgaagccc tcctccgcca ggacgggcag
     7201 gagcttctcg ccgtccgagt cggccctgaa ggactcgatg ccgaggaggc gggggacgct
     7261 agggcccgtg atgtccgcgt ccaggagtcc caccttgagg cccttcgccg cgcaggcctg
     7321 ggccaggagc accgcgctcg tcgacttgcc gacgccgccc ttgccggaac cgaccccgat
     7381 cacgtatgtt accttgttgg cgaccgcgtc aggggcggcg cccttgtcct cgttcatgtg
     7441 agtcctccga tggttctatc gctcgggaag atactaacaa gcatatgccc aatagtcaag
     7501 tatacgagga gacagcccga tgatacaggc tttcacgtcg cacgagcccc tcgaccatct
     7561 tggcctctat ggggccttcc tcgaaaggga gacggactca agcggcacgg ccgtcctcca
     7621 ccacggcagg gtcaagcgcc cgggcaagca agtgcccgat tttaaggcgg tggagctcaa
     7681 gaccctcggt tccggcgcgg acgaggggct cctggccatc gcccgccggg cccgggccga
     7741 cttcgccctc aaccaggtcc tgcttgtcca tcgcctaggc cgcctcgcgg cgggtgatac
     7801 ggtcctcctc gccatcgttt ccggcgcgac tcgggaccgc tgcttcgcgg cctgctcgtg
     7861 gatcgtggac gaggtcaaga aggaagagct catcgagctc gtcgagctgc cgtagcggaa
     7921 cgggctcagg cccgttgtcc ggccggagtc ccggcc
//
LOCUS       DAOOSJ010000117         7842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5796_length_7842_cov_6.009375, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000117 DAOOSJ010000000
VERSION     DAOOSJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5796_length_7842_cov_6.009375"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..1495)
                     /locus_tag="PLG14_07030"
     CDS             complement(425..1495)
                     /locus_tag="PLG14_07030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48928.1"
                     /translation="MQHAESLSATDYSVRSFRPSDLPALTLLTAKAFAADKTWGERRT
                     LTEITLPALLASGPSFDPELVLVAEGRDGGLLGAAVWYPYAMRLRGERIVAANLAPLA
                     VDPEAKGRGLGSALMRASFPVLEGRGASLAFLCGHEAYYPRFGFLKNMFGQFGLRPAR
                     TGDGEQSPDAAGDILRAPRPSDEAALLGLWETCQGSLDFALEPEPGFVSWMAWTPSTQ
                     ALVLERDGKPAAYARFANERPSDAFGGNAEPKATSIRQFLAADAAAAARLLSALGRPL
                     GSGAGETSPYLPVHPDSEAAKRLFPAGFEAVRATGGYAMALPLEGGPARAVRAAAEYC
                     ALVSEGKLEPGLLVLPPIFDLE"
     gene            1769..1841
                     /locus_tag="PLG14_07035"
     tRNA            1769..1841
                     /locus_tag="PLG14_07035"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1802..1804,aa:Phe,seq:gaa)
     gene            1908..2570
                     /locus_tag="PLG14_07040"
     CDS             1908..2570
                     /locus_tag="PLG14_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOX48929.1"
                     /translation="MKAWPVVVVVGQKGSGKSSLGLALAGLSGADFADLDELVAREAS
                     LPTPREAWRALGEAEFRRLEALILRRELEGARTALAGGAEGGPKPGEPGSGAAIPDGG
                     RPRILAAGGGLADNPAALEALAEGARSGRFRLLVLRLPFGLLAERAAASGYPPSLGPE
                     PSPQAFAALAERREGAYLALAGLPGALVLEAAGLSVREAAGRALSLLDLVPADSAPRR
                     QA"
     gene            2567..2959
                     /locus_tag="PLG14_07045"
     CDS             2567..2959
                     /locus_tag="PLG14_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48930.1"
                     /translation="MNVELFALCDYAQEEGGKLSILGLFDTLCAREFPATHPLFCVAA
                     RLRFPVYELGSHEIRFAIRDAEGRDLLPPFEGSISVDGIGIDSAASNIALRLANVPLP
                     GESSWRAILFVDGAEVAHTPLYLRGPRN"
     assembly_gap    2975..3074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3226..4203
                     /gene="galE"
                     /locus_tag="PLG14_07050"
     CDS             3226..4203
                     /gene="galE"
                     /locus_tag="PLG14_07050"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOX48931.1"
                     /translation="MKILVVGGAGYIGSHVARECLDSGHSVTVYDNLSSGSKGNLFPE
                     ASFVEGDILDYSALLSAMRGGYDALVHLAAFKAAGESMLKPEKYSLNNLNGTVNILNA
                     ALEAGVGRVVFSSSAATYGEPKYLPIDEKHPTEPENYYGFTKLEIERILGWYDRLKGL
                     RFAALRYFNAAGYDVRGRISGLERNPANLIPVVMEAACGMRPEVQVFGNDYGTKDGTG
                     VRDYVHVSDLARAHVAALEWIAKNDRSLTVNLGSEEGLSVLEIVEAARRVTGRPVPAK
                     IVGRRPGDPAKLVASSALAKESLGWEARHSDVETLVRTTWEAYRRAGKA"
     gene            4283..5512
                     /locus_tag="PLG14_07055"
     CDS             4283..5512
                     /locus_tag="PLG14_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOX48932.1"
                     /translation="MTNKEKAFAWLDKATPGIVELESLLTAIPAMAPESGGEGELAKA
                     EALEAWLAKKGITKIERFDSKDPRVPSGLRPNIVATIPGERDSGGRVWIMSHTDVVPE
                     GDRGMWKTDPFKVEHRDGRVYGRGVEDNQQGLVSSVFAVLSLLEQGIVPTRTVKLLFV
                     ADEEVGSVHGIQHLLKAADLFRKEDLILVPDGGSADGSEIEVAEKNICWMKVTTKGKQ
                     THASMPDQGANAFLAACDLALRVHALEKEAFPARDPLFHPDRTTIHPTKKEANVPNVN
                     TIPGDDVFYFDMRILPCYSVAQVVAEVEKKMRAVEKDWGVTVSYEIVQKNESKATSPE
                     APVVKELARAVREVYGVEGKAIGIGGGSVAAYLRNAGLDAVVWSRMEETAHQPNESAL
                     LANIIGDAKVFASLMLA"
     gene            complement(5509..>6255)
                     /locus_tag="PLG14_07060"
     CDS             complement(5509..>6255)
                     /locus_tag="PLG14_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48933.1"
                     /translation="LSLSVELDVAGAGLRLPAGRLEAERMVERELPALMKDAVFGLQV
                     DSFRSLGDTIADGSVEVDELLSLAELAERKEAVFSRDLKTFRAVYELPLLAAASLYVR
                     HSSPTALRASMDFRPTKAYTGIVIYAKGELPLRGESGEAEARPCFFPRVFDEEMNLIV
                     ERNAVSPEALARWGEVGYAEGLSLDSLERVGESPLKLVAAAVFGTGRTDLVISRDDAL
                     KILGRPENRALVAEGRVLFVLDEVSGREGR"
     assembly_gap    6256..6355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6356..>7842)
                     /locus_tag="PLG14_07065"
     CDS             complement(<6356..>7842)
                     /locus_tag="PLG14_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX48934.1"
                     /translation="TMHESIGQARLPGEPTRQELYLRLTRGLNIERDYQSWIETWEDY
                     GPTRNSVESLPLTILVLAAVPASAGTSAARPSAREMAPESFARSLDLPAPGGVPEAPL
                     QGHAEEARYGIDMPWGEERFESFRSAYLSEGGRKWLEAVTLRARPYLPYIAERIRFYG
                     LPEELAFLPVIESEYSSKAVSRSGAAGLWQFMKNSIGGYGIRVDDWVDERRDFMKSTD
                     AALRKLADNYAYFGDWPLALAAYNAGLGAVSRAVKKAASAETPAPDYWELRRRGLLSK
                     ETAGYVPKFLAAASILRYPARHGLSVSWEEPEAWEALELAKPVDLVLLSEAASIPLDL
                     LKAANAELRYTVTPPYAGHKVKVPAGRAEAIRAVLDDPARKLVRYHLHKVRSGDTLSA
                     LSRHYGTPVAMILQANPGLRADLLRIGQVLVVPALKEAGPPPAPPRPDGAGIDFSGSY
                     VVKKGDSLWAIALRHEVQPEVLAERNGLTLESVIREGMSLRVPIL"
BASE COUNT         1114 a         2697 c         2760 g         1071 t
ORIGIN      
        1 gtacagctcg atggaatggt cctccgacct cgcctcgtgg cccatgccct tcaggaaggc
       61 ctcgaggtag tggacggcgg ggtagggcgt gttgagctgc acgaggggcg gctggacgag
      121 aagggctcgc atggaaggag tatagcggcg cctccgggag gccgggaagc gggcggggtc
      181 gcggcgctga tgcgcgtccc cgtggcggac tccgcggctc cgggccgtcc gcgccacgtg
      241 cgccgggccg tccgcgccgc gcgcgtctgg cgggcgccgc gggagtcgcc cgcccgcgcc
      301 gccgcctgga aaggccgccg cgccttggcc tcgcgccgcc gcctgtaaaa ccgccgcaaa
      361 aatagccgcc gccccgcggg gcggagcggc ggctcggaat gcctcgccta cgcgaggtcc
      421 cggctcattc caggtcgaag attgggggaa ggacgaggag gccgggctcg agcttccctt
      481 ccgaaacgag ggcgcaatac tccgcggcgg cccggaccgc ccgggccggg ccgccctcca
      541 aggggagggc catcgcgtag ccgcccgtcg cccgcaccgc ctcgaagccc gccgggaaga
      601 gtctctttgc ggcctccgag tcggggtgga cggggaggta ggggctcgtt tccccggcgc
      661 cggagccgag aggccggccg agggccgaga ggaggcgggc ggcggccgcg gcgtcggcgg
      721 ccaggaactg gcgtatggac gtcgccttgg gctcggcgtt gccgccgaag gcgtcgctcg
      781 gcctctcgtt cgcgaagcgg gcataggcgg cgggctttcc gtcgcgctcg aggacgaggg
      841 cctgcgtcga gggagtccag gccatccagg aaacgaagcc gggctcgggc tcgagggcga
      901 agtccaggct cccctggcag gtttcccaga gtccgagaag ggcggcctcg tccgagggcc
      961 gcggggctcg caggatgtcc ccggcggcgt cagggctctg ctcgccgtcg ccggtccggg
     1021 cgggccggag gccgaactgg ccgaacatgt tcttcaggaa gccgaagcgg gggtagtagg
     1081 cctcgtggcc gcagaggaag gcgagggagg cgccccgccc ttcgagtacg gggaacgaag
     1141 cccgcatcag ggccgagccg aggcccctgc ccttggcctc gggatcgact gcgaggggag
     1201 ccaggttggc cgcgacgatg cgctcgccgc ggagccgcat ggcgtaggga taccagaccg
     1261 cggcgcccag gagcccgccg tcccggccct cggcgacgag gacgagctcg ggatcgaagg
     1321 aggggcccga ggcgaggagg gcggggaggg tgatctcggt cagggtgcgg cgctcgcccc
     1381 aggtcttgtc ggccgcgaag gccttggcgg tgaggagggt gagggcgggg aggtcggagg
     1441 ggcggaaact tctgacggaa tagtcggtcg cggagaggga ttctgcgtgc tgcatcgatg
     1501 agctccggat gcggaaccgg aggccccggg ccggcgacgc cggcttggtt cgccgcgtct
     1561 cgcggctgga ggccttccgt tttcagctgg gcagcgcctg cggtcctcct cctgcgaggg
     1621 gccgggcgcc gccttattcc gtcgctttca tgggggcatt ctagccgggg cggccgcgcc
     1681 tgggcaaggc ttataaagat tctgggccaa gtccgcgatc cctcttgacg atcccgcggc
     1741 gttcagctat aagaataacg catcgagcgc cgctgtagct cagttggtag agcagtggac
     1801 tgaaaatcca cgtgtcgtca gttcgattct gaccggtggc acgaaatcgg gccgatccct
     1861 tccggggtcg gccttttcat tttccgcggc cgcgttatga tcgaagtatg aaggcttggc
     1921 cggtcgtcgt cgtcgtcggg cagaagggct cgggaaaatc gagcctgggc ctggccttgg
     1981 ccggcctctc cggggccgac ttcgccgacc tggacgagct tgtcgcccgc gaggcctccc
     2041 tgccgacgcc gcgggaagcc tggcgggccc tgggcgaggc ggaattccgc cggctcgagg
     2101 ccctgatcct ccggcgcgag ctcgaggggg cgcggacggc cctcgcaggc ggcgcggagg
     2161 gcggcccgaa acccggcgag cctggatccg gcgccgccat ccccgacggg ggccggccgc
     2221 ggatcctggc cgcggggggc ggcctcgccg acaatcccgc ggccctcgag gccctcgccg
     2281 agggcgcccg gagcggccgc ttccgtctcc tcgtcctccg ccttcccttc ggcctcctcg
     2341 ccgaaagggc cgcggcctcg ggctacccgc cgagcctcgg cccggagccc tcgccccagg
     2401 ccttcgccgc cctcgcggag cggcgggaag gggcctacct cgccctggcc ggcctgccgg
     2461 gcgccctcgt cctcgaggcc gcggggctat ccgtccgtga agcggccggc cgcgcccttt
     2521 cgctcctcga cctggtcccc gccgattcgg ccccaaggag gcaggcatga acgtcgagct
     2581 cttcgcgctc tgcgactacg cccaggaaga gggagggaag ctctccatcc tgggcctttt
     2641 cgacacgctc tgcgcccggg agttcccggc gacccatccc ctcttctgcg tggccgcccg
     2701 cctgcgcttc ccggtctacg agctcggcag ccacgagatc cgcttcgcca tccgcgacgc
     2761 cgaagggcgg gacctccttc ctcccttcga gggctccatc tccgtggacg gcatcggcat
     2821 cgactccgcc gcctccaaca tcgccctgcg cctggcgaac gtgcccctgc ccggggagag
     2881 cagctggagg gccatcctct tcgtcgacgg ggccgaggtc gcccacacgc cgctctacct
     2941 ccgcggcccg cggaactagt cgagcgcgcc gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnngccggc ggcctcttcg gcttggaggg cgcggcgctt tgggatatac
     3121 tgcgcgagga agccccgccc ggccgggcag ggcgcgcgag cccccctcgg gcccgcgccg
     3181 ccccctcgcc ctccgtccgc ggcctcccat cggaaggagc acaacatgaa gatcctcgtc
     3241 gtcggcggcg ccggctatat cggaagccat gtcgcccgcg aatgcctcga ttcgggccat
     3301 tcggtcacgg tctacgacaa cctctcctcg ggctctaagg gcaacctctt ccccgaggcc
     3361 tccttcgtcg agggcgacat cctcgactat tccgccctcc tctcggcgat gcggggcggc
     3421 tacgacgccc tcgtccacct cgccgccttc aaggccgcgg gcgagtcgat gctgaagccc
     3481 gagaagtact ccctcaacaa cctgaacggc acggtgaaca tcctcaacgc ggccctggag
     3541 gccggcgtcg gccgcgtggt cttctcctcc tccgccgcga cctacggcga gcccaagtac
     3601 ctccccatcg acgagaagca tccgaccgag cccgagaact actacggctt cacgaagctc
     3661 gagatcgagc ggatcctcgg ctggtacgac cgcctcaagg gtctgcgctt cgccgccctg
     3721 cgctacttca acgcggcggg ctacgacgtc cggggccgca tctccggcct ggagcgcaat
     3781 cccgcgaacc tcatccccgt cgtgatggag gccgcctgcg gcatgcgccc cgaggtccag
     3841 gtcttcggga acgactacgg cacgaaggac ggcacgggcg tgcgcgacta cgtccacgtg
     3901 agcgacctgg cccgcgccca cgtcgcggcc ctggagtgga tcgcgaagaa cgacaggagc
     3961 ctcacggtca acctgggctc cgaggagggc ctctccgtcc tcgagatcgt cgaggcggcc
     4021 cgccgcgtca cgggcaggcc cgtcccggcg aagatcgtcg gccgcaggcc aggcgacccc
     4081 gcgaagctcg tcgcctcctc cgccctcgcg aaggagtccc tgggctggga ggcgaggcac
     4141 tccgacgtgg agaccctcgt gcgcaccacc tgggaggcct accgccgggc cggcaaggcc
     4201 taggtcaaga caagcgaacc aagggggagc gccacggctc gcacggcccg ggcgcgcccc
     4261 tcgtccatac aaaggagaac ccatgacgaa caaggaaaag gccttcgcct ggctcgacaa
     4321 ggcgaccccg ggcatcgtcg agctcgagag cctcctcacg gccatccccg cgatggctcc
     4381 cgagtccggg ggcgagggcg agctcgcgaa ggcggaggcc ctggaggcct ggctcgcgaa
     4441 gaagggcatc accaagatcg agcgcttcga ctcgaaggac ccccgcgttc cctcgggcct
     4501 gcgcccgaac atcgtcgcga ccatccccgg cgagcgcgac tcgggcggcc gcgtctggat
     4561 catgtcccac accgacgtcg tccccgaggg cgaccgcggc atgtggaaga ccgatccctt
     4621 caaggtcgag caccgcgacg gccgggtcta cggccgcggc gtggaggaca accagcaggg
     4681 cctcgtctcc tccgtcttcg ccgtcctctc cctcctggag cagggcatcg tcccgacccg
     4741 caccgtgaag ctcctcttcg tcgccgacga ggaggtgggc tcggtccacg ggatccagca
     4801 cctcctcaag gcggcggacc tcttccgcaa ggaggacctc atcctcgtgc cggacggcgg
     4861 ctcggccgac ggctcggaga tcgaggtcgc ggagaagaac atctgctgga tgaaggtcac
     4921 gaccaagggg aagcagaccc acgcctccat gcccgaccag ggcgcgaacg ccttcctggc
     4981 ggcctgcgac ctggccctgc gggtccacgc cctggagaag gaggccttcc cggcccgcga
     5041 ccccctcttc caccccgacc gcacgacgat ccacccgacc aagaaggaag ccaacgtccc
     5101 gaacgtcaac acgatcccgg gcgacgacgt cttctacttc gacatgcgca tcctcccctg
     5161 ctactccgtg gcgcaggtcg tggccgaggt cgagaagaag atgcgcgccg tggagaagga
     5221 ctggggcgtg acggtctcct acgagatcgt ccagaagaac gagtccaagg cgaccagccc
     5281 cgaggccccc gtcgtgaagg agctcgcccg ggcggtgcgc gaggtctacg gggtcgaagg
     5341 gaaggccatc ggcatcggcg gcggctcggt cgcggcctat ctccgcaacg cgggcctgga
     5401 cgccgtcgtc tggtccagga tggaggagac cgcccaccag cccaacgagt cggccctcct
     5461 ggccaacatc atcggcgacg cgaaggtctt cgcgagcctg atgctggcct agcggccttc
     5521 ccgccccgag acctcgtcca ggacgaagag gacccgcccc tcggcgacga gggcgcggtt
     5581 ctcggggcgg cccaggatct tgagcgcgtc gtcccgcgag atcacgaggt cggtgcgccc
     5641 ggtcccgaag accgcggccg cgacgagctt caggggcgac tccccgaccc tctccaggct
     5701 gtccagggag aggccctcgg cgtagcccac ctcgccccag cgcgcgagcg cctcgggcga
     5761 gaccgcgttc cgctccacga tgaggttcat ctcctcgtcg aagacgcggg ggaagaagca
     5821 gggcctggcc tccgcctcgc cgctctcgcc ccgcaggggg agctcgccct tcgcgtagat
     5881 cacgatgccc gtgtaggcct tggtgggacg gaagtccatg gaggcccgca gggccgtcgg
     5941 cgagctgtgc cgcacgtaga gcgaggccgc ggcgaggagg gggagctcgt agaccgcgcg
     6001 gaaggtcttg aggtcgcggg agaagacggc ctccttccgc tccgccagct ccgcgaggga
     6061 gaggagctcg tccacctcca cgctgccgtc ggctatcgtg tcccccaggc tccggaagga
     6121 gtccacctgg aggccgaaga ccgcgtcctt catcagggcg gggagctcgc gctccaccat
     6181 gcgctcggcc tccaggcgcc cggcgggcag cctgaggccg gcccccgcga cgtcgagctc
     6241 gacgctcagg gagagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgag
     6361 tatcggcacg cggaggctca taccttcgcg gatcacgctt tccagcgtga ggccgttccg
     6421 ctccgcgagg acctcgggct ggacctcgtg gcggagggcg atcgcccaga ggctgtcgcc
     6481 cttcttcacg acatagcttc cggagaagtc gatccccgcg ccgtcgggcc tgggcggggc
     6541 cggcggcggg cccgcctcct tcagggcggg gacgaccagg acctggccga tgcggaggag
     6601 gtccgcgcgg agcccgggat tggcctgcag gatcatcgcg acgggcgtgc cgtagtggcg
     6661 ggagagggcg gagagggtgt cgcccgagcg gaccttgtgg aggtggtagc ggacgagctt
     6721 cctggccgga tcgtccagga cggccctgat ggcttcggcc cggccggccg ggaccttcac
     6781 cttgtgcccc gcgtagggcg gggtcaccgt gtagcggagc tcggcgttgg ccgccttgag
     6841 gaggtcgagg gggatggagg ccgcctccga gaggaggacg aggtcgacgg gcttcgcgag
     6901 ctccagggcc tcccaggcct cgggctcctc ccaggagacg gagaggccgt gcctcgcggg
     6961 gtagcgcagg atggaggcgg ccgcgaggaa cttgggcacg tagcccgcgg tctccttgga
     7021 gaggaggccc cgcctcctga gctcccagta gtcgggggcg ggcgtctccg cggaggcggc
     7081 cttcttgacg gcgcgcgaca cggcgcccag gcccgcgttg taggcggcca gggccagggg
     7141 ccagtccccg aagtaggcgt agttgtccgc gagcttccgg agcgcggcgt cggtgctctt
     7201 catgaagtcg cgccgctcgt ccacccagtc gtccacgcgg atgccgtagc cgccgatcga
     7261 gttcttcatg aactgccaga gccccgccgc gccgctccgg gagacggcct tggaggagta
     7321 ctcggactcg atgacgggca ggaaggcgag ctcctccggg aggccgtaga agcggatgcg
     7381 ctccgcgatg tagggcaggt agggcctggc ccggagcgtc accgcctcca gccacttcct
     7441 cccgccctcg gagaggtagg cggagcggaa ggactcgaag cgctcctcgc cccagggcat
     7501 gtcgatgccg tagcgcgcct cctccgcgtg gccctggagg ggcgcctccg ggacgccgcc
     7561 gggggccggg aggtcgaggc tgcgggcgaa ggactcgggg gccatctctc gggccgaggg
     7621 gcgggcggcg gaggtgcccg ccgaggccgg gacggcggcg aggacgagga tggtcagcgg
     7681 cagcgactcg accgagttcc gcgtcggccc gtaatcctcc caggtctcga tccagctctg
     7741 gtagtcgcgc tcgatgttga ggccgcgcgt caggcgcaga tacagctcct gccgcgtcgg
     7801 ctcgcccggg agtcgggctt gaccgatcga ctcgtgcatc gt
//
LOCUS       DAOOSJ010000118         7837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5798_length_7837_cov_6.037522, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000118 DAOOSJ010000000
VERSION     DAOOSJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_7837_cov_6.037522"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLG14_07070"
     CDS             complement(<1..353)
                     /locus_tag="PLG14_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOX48935.1"
                     /translation="MASKVYFADMRATGPKTSTVAKIERLLSALEVGKAVRKGDLTAI
                     KIHFGELGNDSYVSPVFARKAADLVRAAGGKPFFADTNTLYTGSRSNGPDHLETAIAH
                     GFDFSVCGAPVVIADG"
     gene            complement(646..2121)
                     /locus_tag="PLG14_07075"
     CDS             complement(646..2121)
                     /locus_tag="PLG14_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOX48936.1"
                     /translation="MRALVLPHRLLGAFEVPPSKSHAARALLLSAFARGESRIEGFAR
                     PGSPSGGDSGAALRAARAFGAEVLPQADGSLLVRGNGGEPPASGRIDCGNSGTMLYFC
                     SALAALASGEWILDGDAQTRGRPALPLLAALRTLGAEAEGSGPGGGFAPLRLRGPLAS
                     GGGGRPRVRLESPTSQYLSALLLALPFSPRGAELELSLLNERPYVGMTLSWLARAGLE
                     ALPSGGAEPPVGAAAAPGGPAAAASQAAPGFPAAAGGAGDPYSRFLVPGGQTARPFRS
                     RIPADWSAAAFLLAAGAAGGGPVEVQGLSSGDGQGDEAILGMLRAFGAEVSLKQDLRE
                     EGDGQGRGLVAATVSRSGPLRPVDLDLNATPDLLPVLAALAALVPGRSVFRNVPQARI
                     KETDRIAAMAAALGALGARTEELPDGLAVEGPLGSRPATRRGEGGPRPLAVDSYGDHR
                     VAMALAVLGLGAPFPLAIDQAESAAVSYPGFWEAIGAELPE"
     gene            2250..3734
                     /gene="glgA"
                     /locus_tag="PLG14_07080"
     CDS             2250..3734
                     /gene="glgA"
                     /locus_tag="PLG14_07080"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOX48937.1"
                     /translation="MKILMVTSEATPFAKAGGLADAVSALSRALSRAGHDVRILLPRY
                     YSIDRSSLELLEGPLAVPSPGGEEWTAVYKGTLPASEVPVYFLDHERSFGREGIYGDK
                     AQPDFADNPARFALLSRSAFALCRRLGWFPDILHAHDWPTALAPVYLRRLEGGGEFAG
                     TASVLSIHNLGYQGVYDKGCFPALGLPWELFHGAGLEYYDRVNLLKGGVSEADALSTV
                     SPTYAREIQTPELGAGLDGLLRHRSADLVGILNGVDLEDWDPRTDRLIPARYSAKDLS
                     GKAACKAALQRAFGLPEDPAVPVVGMVSRLADQKGIAELFGPSYGSAASICEGMALQF
                     VIQGSGEKWCEAELSSLAARIPNFKARIGYNEATAHLIEAGADFFMMPSRYEPCGLNQ
                     MYSLRYGTLPIVRRTGGLADTVENYDEESGGGTGFMFDDLTPRAVFDTTGWAVWVWYN
                     RPERLRAMRKRAMEKDFSWDRPAGEYAKLYGRALERSAARGWPI"
     gene            3750..5288
                     /locus_tag="PLG14_07085"
     CDS             3750..5288
                     /locus_tag="PLG14_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48938.1"
                     /translation="MATTEKKVYDYKPELVRDRLVEVFRKRKGEATTADLVALTGLPK
                     AQVEAELKPVSDEYGARLRVTESGEILYSFPQGMKSRYRGLGPSLRRFWKAFKKGAAA
                     AAVFLFKIWIVGMLVGYFVLFLALALLAMLASVAVSMSGKDDRSDRRGGGIGGLYLTG
                     RLLDAFVRIWFYSELFKDPYQRSYERGLRAERRREKKPLHRAIFSFVFGDGDPNADWE
                     SVEKKAVVAFLQANKGVVTMPEFMALTGLPPAEAESRINRYLLEFEGEPAVSPGGAIY
                     YFFPGLLRRKDRADRTYGGSLPMKRLAAFSANPKKANAWFCAINGANTLFGGYFLGMS
                     LAAQPLLPILYGGQYADRVVLTRGFDFFYLFTHQLFGKVLGVGDPSALLGLVLGAVPL
                     AFSFFFYLVPVLRSRRLKAANERVKLENLRRVAYGSVLAAPGGVRPESVPVADEAARP
                     RDSRAAERLLKELAAAAGGEPGADGSYAFPEIARGQAEAGRVRAAVREEDYALGAAVF
                     DSDS"
     gene            5305..6213
                     /locus_tag="PLG14_07090"
     CDS             5305..6213
                     /locus_tag="PLG14_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX48939.1"
                     /translation="MAKTSRASGVILALLSAAGFSTLGVFAKLVYAEGLSVPEVLAWR
                     FTVAALVLWAIVLLGRRPLPRRLLPVLLLGLLGFAPQAGLYFLTIRILDPGITSLLLY
                     TYPSFVVLFSFLLFRRRPTRVQLAALGLSLAGCVVTFWRRGDYPMGGLLLGAFVGLAY
                     GAYLVAGEKVLREVDSISATALIMVAAAGVYWAFALAQAAAGGRAPRLPSSPMAAGGI
                     VGIALLATVLPITALFGSMRRIGAADASLVSTLEPVLTVGLSALVLGERLGPAQLAGG
                     LLILAAVVALNLPERAPKAAPPRRAD"
     gene            complement(6302..7285)
                     /locus_tag="PLG14_07095"
     CDS             complement(6302..7285)
                     /locus_tag="PLG14_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOX48940.1"
                     /translation="MEYRRLGGAGVKVSVLSLGSWVTYGKQLGIDAAAASIKAAYDQG
                     VNFFDNAESYEGGKAEEIMGAALKKLGLRRGSYLVSTKVFWGLHEGPNEKDTLNRKRL
                     LEAIDGSLRRFGLDYVDFLYCHRPDPDTPIEETVWAMSDIVASGRALYWGTSEWSAEE
                     LREAAGIADRRNLRQPRMEQPQYNLFVRERVEGEYARLYDGLGLGLTTYSPLASGVLT
                     GKYDGGVPSGSRLSLPGYEWLKSSAISPGRIEAARRLGPVAEELGATRSQLALAWCAK
                     NPRVSSVITGASRPEQVLENMKAVELLPRLDEGVMARIEEATAAASDRKAR"
     gene            7367..7576
                     /locus_tag="PLG14_07100"
     CDS             7367..7576
                     /locus_tag="PLG14_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOX48941.1"
                     /translation="MRISARNVLRGKVQKVVEGAVNVEVLLKLDGGEEVTSIITKASA
                     ERLGLAVGKVAYAVVKASDVMIGVD"
     gene            complement(7604..>7837)
                     /locus_tag="PLG14_07105"
     CDS             complement(7604..>7837)
                     /locus_tag="PLG14_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48942.1"
                     /translation="GYAKNCGEELWRRADRRTKRLFEREGPLLYPRCAEVDYPDLFRA
                     ALGKGLLLSTDWSLPSLLPVDFDDGELKPLAEL"
BASE COUNT         1040 a         2880 c         2771 g         1146 t
ORIGIN      
        1 ccgtccgcga tgacgaccgg ggcgccgcag acggagaagt cgaagccgtg ggcgatggcg
       61 gtctccaggt ggtccgggcc gttcgagcgg ctccccgtgt agagggtgtt cgtgtccgcg
      121 aagaagggct tgcctcccgc cgcgcgcacg aggtcggcgg ccttgcgggc gaagacgggg
      181 ctcacgtagg agtcgttgcc gagctccccg aagtggatct tgatcgcggt gaggtcgccc
      241 ttcctgacgg ccttgccgac ctcgagggcg gaaaggaggc gctcgatctt ggcgacggtc
      301 gaggtcttgg ggcccgtcgc gcgcatgtcg gcgaagtaga ctttgctggc catgggtatc
      361 tccttaaggt tcaattttcc agcggtcgcg gtcccgcgcc ttcgcgttgg ccgcctcgtg
      421 cgcgggcgac ttcgcgccgc cgcccgcgct tgcgcgacag tcgcccgcgc ttgcgcgata
      481 gccgcccgtt gccgcgcctt ggccgctgcg cgatagccga acgcacagag ccgcctgcgc
      541 agagccggcc gcgatagccg ctcgcggccg cgccgcctcc tgggccgcgt ccgcccgccc
      601 ggagccgcct gcgcggcggc cggacggcgc ccaggctgtc attgcctatt ccgggagctc
      661 ggctccgatg gcctcccaga atccgggata ggatacagcc gcagactccg cttggtctat
      721 cgcgaggggg aagggcgcgc ccaggcccag gacggccagg gccatagcga cgcggtggtc
      781 gccgtatgag tcgacggcga gcggcctcgg gccgccctcg ccgcgccgcg tcgccggtct
      841 ggatcccaag ggcccctcga cggccaggcc gtcggggagc tcctcggtcc gggcgcccag
      901 ggcgcccaag gcggcggcca tcgcggcgat gcgatccgtc tccttgatgc gcgcctgggg
      961 cacgttccgg aagacgctcc ttccggggac aagggccgcg agggccgcga ggacggggag
     1021 gaggtctgga gtcgcgttca ggtcgaggtc gacggggcgc aaggggcccg agcgcgagac
     1081 ggtcgcggcg acgaggccgc ggccctggcc gtcgccctcc tcgcggaggt cctgcttcag
     1141 cgagacctcg gccccgaagg ccctgagcat gccgagtatc gcctcgtcgc cctggccgtc
     1201 gcctgaggag aggccttgga cctcgacggg cccgccgccc gcggcgccgg ccgcgaggag
     1261 gaaggcggcg gccgaccagt cggcggggat gcgggagcgg aaggggcggg ctgtctggcc
     1321 gccgggcacg aggaagcgtg agtagggatc gcccgcgccg ccggccgcgg cggggaagcc
     1381 aggcgccgcc tgactggcgg ccgcggccgg gccgccaggc gccgcggccg cgccaaccgg
     1441 gggctccgcg ccgccggagg gcaaggcctc gaggcccgcc cgcgcgagcc aggagagggt
     1501 catgccgacg tagggcctct cgttcaggag ggagagctcg agctcggcgc cgcgggggga
     1561 gaaggggagg gcgaggagga gggcggagag gtactggctc gtcggggatt cgaggcggac
     1621 gcgggggcgg ccgcctccgc ccgaggcgag ggggccgcgg aggcgcaagg gggcgaagcc
     1681 gccgcctggg ccggagccct cggcctcggc gccgagggtc cgcagggcgg cgaggagggg
     1741 gagggcgggc ctgcccctcg tctgcgcgtc gccgtccagg atccactcgc ccgaggcgag
     1801 ggccgccagg gccgagcaga agtagagcat cgtgcccgag ttgccgcagt cgatccggcc
     1861 cgaggcgggc ggctctccgc cgttgccgcg gacaaggagg cttccgtcgg cctggggcag
     1921 gacctcggcc ccgaaggcgc gggcggcgcg cagggccgcg cccgagtccc cgcccgaggg
     1981 agagcccggc ctcgcgaagc cctcgatcct gctctcgccg cgggcgaagg cggaaagaag
     2041 gagggcgcgg gcggcgtggg acttggaggg cgggacctcg aaggcgccaa ggaggcgatg
     2101 gggcaggacg agggctctca cgccgaaagc ctacgcaagg ggcggcgcgt tgcgcaaggg
     2161 ggctttcccg tcgtataatc gcccgttcct tccccgcgcc ggcgaaacgg ccgcggccgg
     2221 agggagcgtt cgaaaggagc gatcccgcct tgaagatcct catggtgacg agcgaggcga
     2281 cacccttcgc gaaggcgggc ggcctggccg acgccgtctc tgccctctcg cgcgccctct
     2341 cccgggccgg gcacgatgtc cggatcctcc tcccgcgcta ctactcgatc gaccgctcct
     2401 ccctcgagct cctggaaggc cccctcgccg tgccgtcccc gggcggcgag gaatggacgg
     2461 ccgtctacaa ggggactctc ccggcctccg aggtcccggt ctacttcctg gaccacgagc
     2521 gctccttcgg gcgcgagggc atctacggcg acaaggccca gcccgacttc gccgacaacc
     2581 ccgcgcgctt cgccctcctg tcccgctcgg ccttcgccct ctgccgcagg ctcggctggt
     2641 tccccgacat cctccacgcc cacgactggc ccacggccct ggcgccggtc tacctccgcc
     2701 gcctggaggg cggcggggag ttcgcgggca ccgcgagcgt cctctcgatc cacaacctgg
     2761 gctaccaggg cgtctacgac aagggctgct tccccgcctt aggcctcccc tgggagctct
     2821 tccacggcgc gggcctcgag tactacgacc gcgtgaacct cttgaagggc ggcgtctcgg
     2881 aggccgacgc cctctccacg gtctcgccga cctacgcccg ggagatccag accccggagc
     2941 tcggcgcggg cctggacggc ctcctccgcc accggagcgc cgacctggtc ggcatcctga
     3001 acggcgtcga cctggaggac tgggacccga ggaccgaccg cctcatcccc gcgcgctact
     3061 cggcgaagga cctctcgggc aaggccgcct gcaaggccgc gctccagcgc gccttcggcc
     3121 tcccggagga tcccgcggtc cccgtggtcg gcatggtctc gcgcctggcc gaccagaagg
     3181 gcatcgccga gctcttcggc ccctcctacg gcagcgccgc ctcgatctgc gagggcatgg
     3241 ccctccagtt cgtcatccag ggctcgggcg agaagtggtg cgaggcggag ctctcctccc
     3301 tggccgcgcg gatccctaac ttcaaggcga ggatcggcta caacgaggcg acggcccacc
     3361 tcatagaggc cggcgccgac ttcttcatga tgcccagccg ctacgagccc tgcggcctca
     3421 accagatgta ctcgctccgc tacggcaccc tccccatcgt ccgccggacg ggagggctcg
     3481 ccgacacggt ggagaactac gacgaggagt cgggcggcgg cacgggcttc atgttcgacg
     3541 acctcacgcc ccgcgcggtc ttcgacacga cgggctgggc ggtctgggtc tggtacaaca
     3601 ggcccgagcg gctgcgcgcg atgcggaagc gggcgatgga gaaggatttc tcctgggaca
     3661 ggccggcggg cgagtacgcg aagctctacg gccgtgcgct cgagcggagc gccgcgcggg
     3721 gctggcccat ctaggcaagc ggaggaacga tggcgacgac ggagaagaag gtctacgact
     3781 acaagcccga gctcgtcagg gacaggctcg tggaggtctt caggaagcgg aagggcgagg
     3841 cgacgacggc cgacctcgtg gccctcacgg gcctccccaa ggcccaggtc gaggcggagc
     3901 tcaagcccgt ctccgacgag tacggcgccc gcctccgcgt caccgaatcg ggcgagatcc
     3961 tctactcctt cccccagggg atgaaaagcc gctaccgggg cctcggcccc tccctgcggc
     4021 gcttctggaa ggccttcaag aagggcgcgg ccgcggcggc ggtcttcctc ttcaagatct
     4081 ggatcgtcgg gatgctcgtg ggctacttcg tcctcttcct ggccctcgcc ctcctcgcga
     4141 tgctcgcctc cgtcgccgtc tccatgtcgg ggaaggacga ccgctccgac cgccgcggcg
     4201 gcggcatcgg cggcctctac ctgacgggcc gcctcctgga cgccttcgtc cgcatctggt
     4261 tctactccga gctcttcaag gacccctacc agcgctccta cgagcggggc ctccgggccg
     4321 agcggcgccg cgagaagaag ccgctccacc gggcgatctt ctccttcgtc ttcggcgacg
     4381 gcgacccgaa cgccgactgg gagagcgtgg agaagaaggc cgtcgtcgcc ttcctccagg
     4441 cgaacaaggg cgtcgtcacg atgcccgaat tcatggccct cacgggcctg ccccccgccg
     4501 aggcggagag ccggatcaac cgctacctcc tcgagttcga gggggagccc gcggtgagcc
     4561 ccggcggggc gatctactac ttcttcccgg gcctccttag gcggaaggac agggccgaca
     4621 ggacctacgg cgggagcctc cccatgaagc gcctcgcggc cttctccgcg aacccgaaga
     4681 aggccaacgc ctggttctgc gcgatcaacg gcgccaacac cctcttcggc ggctacttcc
     4741 tgggcatgtc cctcgcggcc cagcccctcc tcccgatcct ctacggcggc cagtacgccg
     4801 accgcgtcgt cctcacccgc ggcttcgact tcttctacct cttcacgcac cagctcttcg
     4861 gcaaggtcct gggcgtcggc gacccctccg ccctcctcgg actcgtcctc ggcgccgtgc
     4921 ccctggcctt ctccttcttc ttctacctcg tgcccgtcct gcgctcgcgc cgcctcaagg
     4981 ccgccaacga gcgggtgaag ctcgagaacc tccgccgggt cgcctacggc tcggtcctgg
     5041 ccgcgcccgg gggcgtcagg cccgagtccg tccccgtcgc cgacgaggcc gcgcggcccc
     5101 gcgactcccg cgccgcggag aggctcctca aggagctcgc ggccgcggcc ggcggcgagc
     5161 ccggggccga cggctcctac gccttcccgg agatcgcgcg cggccaggcc gaggccggcc
     5221 gcgtccgcgc cgccgtccgg gaggaggact acgccctggg cgccgccgtc ttcgactcgg
     5281 actcctgaag cgaaggcgct ggtcatggca aaaacctctc gggcctcggg ggtgatcctc
     5341 gccctcctct ccgcggcagg cttctccacc ctcggcgtct tcgcgaagct cgtctacgcg
     5401 gagggcctct ccgtgccgga ggtcctggcc tggcgcttca cggtcgcggc cctcgtgctc
     5461 tgggccatcg tcctcctggg gcggcgaccc cttccgcgca ggctcctgcc cgtcctcctc
     5521 ctcggcctcc tcggcttcgc gccccaggcc ggcctctact tcctgaccat ccgcatcctg
     5581 gatcccggga tcacgagcct cctcctctac acctatccat ccttcgtcgt cctcttctcc
     5641 ttcctcctct tccgcaggcg gccgacgcga gtccagctcg ccgccctcgg cctctccctc
     5701 gcaggctgcg tcgtcacctt ctggcgccgg ggcgactatc cgatgggcgg cctcctcctc
     5761 ggggccttcg tcggcctcgc ctacggcgcc tacctcgtgg cgggagagaa agtgctgcgg
     5821 gaggtcgatt ccatctcggc cacggccctc atcatggtgg cggcggccgg cgtctactgg
     5881 gccttcgccc tggcccaggc cgcggccggg ggaagggccc cgcgccttcc ctcgagcccc
     5941 atggcggccg gcggcatcgt cgggatagcc ctcctcgcga cggtcctccc gatcaccgcc
     6001 ctcttcggct cgatgcggag gatcggcgct gcggacgcct ccctcgtatc cacgctggag
     6061 cccgtcctca cggtcggcct ctccgccctg gtcctgggcg agcggctcgg gcccgcccag
     6121 ctcgcgggcg gcctcctcat cctagcggcc gtggtggcgc tcaacctgcc cgagcgcgcc
     6181 cccaaggcgg cgccgccgcg gcgggccgac taggccggga agcgccccga agtcggcctg
     6241 gcgcgccgcg ccggcgccgc cgcggcgggc cgaccaggcc ggggccgcgc ccgaaggccg
     6301 cctagcgcgc cttccggtcg ctcgcggcgg ccgtcgcctc ctcgatcctc gccatgaccc
     6361 cctcgtcgag cctcggaagg agctcgacgg ccttcatgtt ctcaaggacc tgctcggggc
     6421 ggctcgcccc cgtgatcacg gaggagacgc gcgggttctt cgcgcaccag gccagggcga
     6481 gctggctccg cgtggcgccg agctcctccg cgacgggccc gagccgcctc gccgcctcga
     6541 tcctgccggg actgatcgcg ctcgacttga gccactcgta gccggggagg gagagccgcg
     6601 agcccgaggg cacgcctccg tcgtacttgc ccgtgaggac gccggaggcg agggggctgt
     6661 aggtggtgag cccgaggccc aggccgtcgt agagcctcgc gtactcgccc tccacccgct
     6721 cgcggacgaa gaggttgtac tggggctgct ccatccgcgg ctggcggagg ttccgccggt
     6781 ccgcgatccc ggcggcctcg cggagctcct cggcgctcca ctcgctcgtt ccccagtaca
     6841 gggcgcggcc ggaggcgacg atgtcgctca tcgcccagac ggtctcctcg atcggcgtgt
     6901 ccggatcggg gcggtggcag tagaggaagt ccacgtagtc gaggccgaag cgcctcaagg
     6961 agccgtcgat cgcctccagg aggcgcttcc ggttcagcgt gtccttctcg ttcggcccct
     7021 cgtggaggcc ccagaagacc ttcgtggaga cgaggtagga gccgcgccta aggccgagct
     7081 tcttcagcgc cgcgcccatg atctcctccg ccttgccgcc ctcgtaggac tcggcgttgt
     7141 cgaagaagtt gaccccctgg tcgtaggcgg ccttgatgga ggcggccgcc gcgtcgatgc
     7201 cgagctgctt gccgtaggtc acccaggagc cgagggagag gaccgagacc ttgacgccgg
     7261 cgccgccgag ccttctgtat tccatgggat ctcctttagg ccgccgcggg gcccgcgaaa
     7321 tcgctatact ctatagtaac ggaaaaagcc tccgtgagga gggcctatgc ggatcagcgc
     7381 gcggaatgtc ctcaggggca aggtccagaa ggtggtcgag ggagccgtga atgtcgaggt
     7441 gctcctgaag ctcgacggcg gcgaggaggt gacgtccatc atcacgaagg cctcggccga
     7501 gaggctgggc ctggccgtcg ggaaggtggc ctacgccgtc gtcaaggcca gcgacgtgat
     7561 gatcggcgtc gactgaaggc tgccccctcc cgcgggcgcc ggtctagagc tccgcgaggg
     7621 gcttgagctc cccgtcgtcg aagtcgaccg gcaggaggga ggggaggctc cagtcggtgg
     7681 agagaaggag tccctttccc agggccgccc tgaagaggtc agggtagtcc acctcggcgc
     7741 atcgagggta gaggaggggg ccttcgcgct cgaaaaggcg cttggtccgc ctgtcggcgc
     7801 ggcgccagag ctcctcgccg cagttcttcg cgtagcc
//
LOCUS       DAOOSJ010000119         7834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5808_length_7834_cov_5.568068, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000119 DAOOSJ010000000
VERSION     DAOOSJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_7834_cov_5.568068"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLG14_07110"
     CDS             complement(<1..437)
                     /locus_tag="PLG14_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX48943.1"
                     /translation="MLDKDLIDAAQGALPCDLNLANCSLVDILRGEIRRDVTVSIRKG
                     HVVGVDDGLRAERTIDLKGLYLAPGLVDAHVHIESSMLTPPEYARIVCPRGTTSVVAD
                     PHEIANVLGYDGMRFMLAASEGLPVDVYFMVPSCVPATGFDTAG"
     gene            566..1858
                     /locus_tag="PLG14_07115"
     CDS             566..1858
                     /locus_tag="PLG14_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOX48944.1"
                     /translation="MEKFFKLKANGTTVRTELVAGLTTFMTMAYILAVNPATLAAAGM
                     DKGALFTATALSSLVATLMMALYANLPFALAPGMGLNAFFAYTVVLAMGYPWELALTA
                     VFVEGIIFILLSIFKIREAIVRAIPLNIRRAVSVGIGLFIAFIGLQNAKIIVSNPAVL
                     VGLGDITKGPALLALIGLVLTGALLAFKVKGALLIGILGTTVIGIPMGITAIPQGFSP
                     LAMPASLSPILFKFQFDKLLTLDFFVVMFSFLFVDMFDTIGTLVGVSTKAKMISSDGT
                     MPRMNRALMSDAVGTVFGACIGTSTVTTYVESAAGVSEGGRTGLTALSTAGLFFVALF
                     LSPIFLLIPGAATAPALILVGLFMMEPIKDIDLSDYTEAIPAFLAIIMMPLTYSIADG
                     IMFGIMGYAIIKVCSGKWKDVPVPTFILAVLFVLKLVL"
     gene            complement(2149..3099)
                     /locus_tag="PLG14_07120"
     CDS             complement(2149..3099)
                     /locus_tag="PLG14_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48945.1"
                     /translation="MLLIASILTIALRAGTSLIYATIGETFTERSGILNLGLEGIMLM
                     GALSAFATAYYSGSVALALLVALAVGAALAAVHAFLTVTMRANQVVSGLSITLFGSGL
                     ASFLGQRLGPAANNGRLVGLVGPRLAPVELPFLSGIPVLGALFRQDVLTYALYALIPL
                     AWFYLYKTRAGLSLRAVGEDPQTADAMGVDVFRTKYLYTIAGGALVALGGAQLSLSYT
                     PGWSENITGGRGWIVIALVIFSMWNPARAIWGALLFGGINAIQFRLQASGTTIPAGFL
                     NMLPYLATIVVLVAMTFWESLSKRIGAPAALGTSYMREEK"
     gene            complement(3100..4278)
                     /locus_tag="PLG14_07125"
     CDS             complement(3100..4278)
                     /locus_tag="PLG14_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48946.1"
                     /translation="MRLHFEKRKTLSVRALFIVPLASLAVSLVLTGLILLLSKADPFA
                     TYAAMFKGAFGSWRAFTETLVKAVPLMLTGLGVSVAFKLKFWNIGAEGQLTVAGVATA
                     WVALFWSPWLPPALLLPVALLVGALAGAAWAGIPAFLKTSLKVDETLVTLMLNYVAIL
                     FSEFLYYGPWRDPKGYGFPGTAPFPEAAWLPRIAGRAHAGLYAGLAAALVLWVVLYRT
                     KWGFELSIIGNSQRAARYQGISVRKNIFLAVLLSGALCGLAGSFEVTGISHRLQQGLS
                     IGYGYTAIIVAWMAQLNPLAVPFVSLLLAGLLVGGDQVQMVMGLPAAMGLVLQGMILF
                     PMLAGSLFTEYRVRLSRAEGPTAVALAAAVPAAAQAPAPEAAAQAPASEPSQSEGGTN
                     "
     gene            complement(4294..5877)
                     /locus_tag="PLG14_07130"
     CDS             complement(4294..5877)
                     /locus_tag="PLG14_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48947.1"
                     /translation="MKDKEGGPAPRAARSPRAACSPGEGGEGRLVEMRGIRKSFPGVQ
                     ASDGIDLRLERGEVLALLGENGAGKSTLMNILVGLYRPDSGEILVRGARAEIESPRDA
                     SALGIGMVHQNFMLVDSMTVAENVVLGLRGLPFVPDMKAAKKEILRLSECYNLKVDPD
                     AFIWQLSVGEQQRVEILKLLYRDAEILVLDEPTAVLTPQEAEELSAVVHRMRSEGKSS
                     IFITHKMEEVMSFSDRVMVLRKGKVVAEKATKDTDPKELARLMVGREVLFRVEKGPFS
                     PGATVLELKGVSALDDRGLPALREVDLRIRAGEILGVAGVAGNGQRELAEVITCLRSA
                     KAGSVSINCVDTTGMSPLEVIQSGVAHIPQDRNQVGAVGDMSVASNLAMKGYRQKPLS
                     AAGLIRPSRFRQLAVRLIDLFKISTPSPETRIKFLSGGNIQKSILAREIGACGSLLVA
                     VYPSRGLDIGATEAVRKELVAERDKGKAVLLVSEDIDELISVADTIAVLFEGRVMGLF
                     PSAEADAERIGALMAGMKA"
     gene            complement(6053..7153)
                     /locus_tag="PLG14_07135"
     CDS             complement(6053..7153)
                     /locus_tag="PLG14_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX48948.1"
                     /translation="MKRKFAVACVAVLSLALALAGCTKKAAAPAAAEAKPFKVAFVYI
                     GPPGDLGWTYEHDRARKAVVETYGDKIQTAYMENVPEGPDAARVIRQYAQEGYDMIFA
                     TSFGYMDPMLEVAKEFPKVKFEHCAGYKTAENMATYFGRIEEARYLSGIIAGKATKSN
                     KIGYVAAFPIPEVVRGINGFTLGLKSVNPKAEVRVVWTNTWYDPVKEREAAVALLDAG
                     CDVIAQHQDTVEPQKAAQERGKLSIGYDSDMGKFVGDSVLASPMWNWAVYYKDTIDKA
                     MKGEWKTHQYWEGLSAGVVALSDMSAKVPEDTRKLVAEAQEKIASGKMNIFAGPIKDQ
                     SGALAVKEGEILDDGKQLSMDWFVQGVVGKVK"
     gene            complement(7416..>7834)
                     /locus_tag="PLG14_07140"
     CDS             complement(7416..>7834)
                     /locus_tag="PLG14_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX48949.1"
                     /translation="AVPGAVPGAAAPAASVPAAAVQPAAKPAATAPAPAAKPAAKAVP
                     AKAAPTKTVRVDEYWIQAASFTSRARADELKESLGRRGLAALITVKDISGKSWYRVRV
                     GPYAKKSEAEGWLGRIKAVPGCEEAGIWTTSVERTL"
BASE COUNT         1190 a         2590 c         2797 g         1257 t
ORIGIN      
        1 cccgcggtgt cgaagcccgt cgcgggcacg caggagggca ccatgaagta gacgtcgacg
       61 gggaggccct cgctggccgc gagcatgaag cgcatgccgt cgtagccgag gacgttggcg
      121 atctcgtggg ggtcggccac cacggaggtc gtgcccctcg ggcagacgat gcgcgcgtac
      181 tcgggggggg tgagcatcga ggactcgatg tggacgtggg cgtcgacgag gccgggcgcc
      241 aggtagaggc ccttcaggtc gatcgtgcgc tcggcgcgga ggccgtcgtc gacgccgacg
      301 acgtggccct tgcggatcga cacggtgacg tcccgccgga tctccccgcg gaggatgtcg
      361 acgagcgagc agttggcgag attgaggtcg cagggcaggg ctccctgcgc cgcgtcgatg
      421 aggtccttgt cgagcatacc gctactctaa accgcatccc gggagcgcgc aagggaaaac
      481 ttggatacgg tatacaaaaa aagcttgcgc atccgccgat gtgaccctac actgatcccc
      541 agtcagtacc taggaggctt ctcgtatgga gaaattcttc aagctcaagg cgaacggcac
      601 gaccgtccgc accgagctcg tcgcgggtct cacgaccttc atgaccatgg cctacatcct
      661 cgcggtcaac ccggcgaccc tagcggccgc cggcatggac aagggagcgc tcttcacggc
      721 gacggcgctc tcctccctcg tcgcgaccct gatgatggcc ctctacgcca acctgccctt
      781 cgcgctcgcc ccgggcatgg gcctcaacgc cttcttcgcc tacacggtcg tcctcgcgat
      841 gggctatccc tgggagctcg ccctcaccgc cgtcttcgtg gaaggcatca tcttcatcct
      901 cctctcgatc ttcaagatcc gggaggccat cgtccgcgcc atcccgctca acatccgcag
      961 ggcggtctcc gtcggcatcg gcctcttcat cgccttcatc ggcctccaga acgcgaagat
     1021 catcgtctcc aaccccgccg tcctcgtcgg cctcggcgac atcacgaagg gccccgccct
     1081 cctcgccctc atcggcctcg tcctcacggg ggccctcctc gccttcaagg tgaagggcgc
     1141 cctcctcatc ggcatcctcg gcacgacggt catcggcatc cccatgggca tcacggcgat
     1201 cccccagggc ttctcgcccc tcgcgatgcc cgcctccctc tcccccatcc tcttcaagtt
     1261 ccagttcgac aagctcctca ccctcgactt cttcgtcgtg atgttcagct tcctcttcgt
     1321 cgacatgttc gacaccatcg gcaccctcgt cggcgtctcc accaaggcga agatgatctc
     1381 ctccgacggc accatgccca ggatgaacag ggccctcatg tccgacgccg tcggcacggt
     1441 cttcggcgcc tgcatcggca cctccacggt cacgacctac gtcgagtccg cggccggcgt
     1501 ctccgagggc ggccgcaccg gcctcaccgc cctctccacc gcgggcctct tcttcgtcgc
     1561 cctcttcctc tcgccgatct tcctcctcat cccgggcgcc gcgaccgccc ccgccctcat
     1621 cctcgtcggc ctcttcatga tggagccgat caaggacatc gacctctccg actacaccga
     1681 ggcgatcccc gccttcctcg ccatcatcat gatgcccctc acctacagca tcgcggacgg
     1741 catcatgttc ggcatcatgg gctacgcgat catcaaggtc tgctccggga agtggaagga
     1801 cgtgcccgtc cccaccttca tcctcgccgt gctcttcgtc ctgaagctcg tcctctgacg
     1861 cggccgggtt tctagacaag taaaggggcc gtcccgcgag gggcggcccc ttcttttatg
     1921 tcgaggcgat ctccccgctc ggcttcgggg aatggagggc aagacggccg aacctctccc
     1981 cggccgatcc aaagaatagg ccgccccaag ggcggccagg cggaaccagg cggaaagagg
     2041 cgcggcatcg gaggagcggc gcgggcttcg tggcgcgggc ttcgtggcgc gccggaccgc
     2101 gccggaccgc gcggggccgt ggaggcgccg cccggggccc taagcccgct acttctcttc
     2161 ccgcatgtag gaggtgccga gggcggccgg cgcgccgatg cgcttggaga gcgactccca
     2221 gaaggtcatg gcgacgagga cgacgatggt cgccaggtag ggcagcatgt tgaggaagcc
     2281 cgccgggatc gtcgtgccgg aggcctggag cctgaactgg atcgcgttga tgccgccgaa
     2341 gaggagggcg ccccagatcg cgcgggccgg attccacatc gagaagatga cgagggcgat
     2401 gacgatccag ccgcgtcccc ccgtgatgtt ctccgaccag cccggcgtgt aggagaggga
     2461 gagctgggcg ccgcccaggg cgaccagggc gccgcccgcg atcgtgtaga ggtacttcgt
     2521 cctgaagacg tcgacgccca tcgcgtcggc cgtctgcgga tcctcgccca cggcgcggag
     2581 cgagaggccg gcgcgggtct tgtagaggta gaaccaggcg agcgggatga gggcgtagag
     2641 cgcgtaggtg aggacgtcct gcctgaagag tgcccccagg acgggtatgc ccgagaggaa
     2701 ggggagctcg acgggggcga gcctcgggcc gacgaggccc acgaggcggc cgttgttcgc
     2761 ggcgggaccc aggcgctggc ccaggaagct cgcgaggccg gagccgaaga gggtgatgga
     2821 gaggccgctc acgacctggt tggcgcgcat cgtcaccgtg aggaaggcgt ggaccgcggc
     2881 cagcgcggcg ccgacggcga gggcgacgag gagggccagg gcgacgctgc ccgagtagta
     2941 ggcggtcgcg aaggccgaga gtgcgcccat gagcatgatg ccctcgaggc cgaggttgag
     3001 gatgcccgag cgctcggtga aggtctcgcc gatcgtcgcg tagatgaggg aggttcccgc
     3061 ccggagggct atcgtgagga tggaggcgat gaggagcatc tagtttgtcc ctccttcgga
     3121 ctggctgggc tcggaggcgg gggcctgcgc ggccgcttcg ggagcggggg cctgcgcggc
     3181 cgcggggacg gccgccgcca gggcgacggc cgtggggccc tcggcgcggg agaggcggac
     3241 gcggtactcg gtgaagaggg agcccgcgag catcgggaag aggatcatgc cctggaggac
     3301 gaggcccatc gcggcgggga ggcccatcac catctggacc tggtcgccgc ccacgaggag
     3361 gcccgcgagg aggagggaga cgaagggcac ggccaggggg ttgagctggg ccatccaggc
     3421 cacgatgatc gccgtgtagc cgtagccgat ggagaggccc tgctggaggc ggtgggagat
     3481 gcccgtcacc tcgaaggagc ccgcgaggcc gcagagcgcg cccgagagga ggacggcgag
     3541 gaagatgttc ttcctgaccg agatgccctg gtagcgcgcg gcgcgctggc tgttcccgat
     3601 gatggagagc tcgaagcccc acttggtcct gtagagcacc acccagagga cgagggccgc
     3661 ggccaggccc gcgtagagcc ccgcgtgggc ccgccccgcg atgcgcggca gccaggcggc
     3721 ctcggggaag ggcgcggtgc ccgggaagcc gtagcccttg gggtcgcgcc aggggccgta
     3781 gtagaggaac tcggagaaga ggatggcgac gtagttgagc atcagggtga cgagggtctc
     3841 gtccaccttg aggctggtct tgaggaaggc cggaatgccc gcccaggcgg cgcccgcgag
     3901 ggccccgacg aggagggcta cggggaggag gagggccggc ggcagccagg ggctccagaa
     3961 gagggccacc caggccgtcg cgacgccggc cacggtgagc tggccctccg cgccgatgtt
     4021 ccagaacttg agcttgaagg ccacggagac gccgaggccc gtcagcatga ggggcacggc
     4081 cttcacgagg gtctcggtga aggccctcca gcttccgaag gctcccttga acatcgcggc
     4141 gtaggtcgcg aagggatcgg ccttggagag gaggaggatg agccccgtga ggacgaggga
     4201 cacggcgagg gaggccaggg gcacgatgaa gagggccctg acggagagcg tcttgcgttt
     4261 ctcgaagtga agtctcatgc gctcgtcctt tccctatgcc ttcatgccgg ccatgagggc
     4321 gcctatcctc tccgcgtcgg cctcggccga ggggaagagg cccatgacgc ggccctcgaa
     4381 gagcacggct atcgtgtccg cgacggagat gagctcgtct atatcctcgg agacgaggag
     4441 gacggccttg cccttgtcgc gctccgcgac gagctccttg cggaccgcct ccgtggcgcc
     4501 gatgtccagg ccccgcgagg ggtagaccgc gacgaggagg gagccgcagg ccccgatctc
     4561 gcgggccagg atggacttct ggatgttgcc gccggagagg aacttgatcc tcgtctcggg
     4621 cgagggggtc gagatcttga agaggtcgat gaggcggacg gcgagctggc ggaagcggga
     4681 gggcctgatg aggccggccg cggagagggg cttttggcgg tagcccttca tcgcgaggtt
     4741 ggaggccacg ctcatgtcgc ccaccgcgcc cacctggttc ctgtcctgcg ggatgtgcgc
     4801 cacgccgctt tggatgacct cgagggggct catgcccgtc gtgtcgacgc agttgatcga
     4861 cacggagccg gccttcgcgg acctgaggca ggtgatgacc tcggcgagct cgcgctggcc
     4921 attgccggcg acgcccgcca cgcccaggat ctcgcccgcg cggatcctga ggtcgacctc
     4981 gcgcagggcc ggaaggcccc ggtcgtccag ggccgagacg cccttgagct cgaggaccgt
     5041 ggcgccgggc gagaaggggc ccttctcgac ccggaacagg acctcgcgcc cgaccatgag
     5101 ccgcgcgagc tccttcgggt ccgtgtcctt cgtcgccttc tccgctacga ccttgccctt
     5161 ccgcaggacc atcacgcggt cggagaagga catgacctcc tccatcttgt gggtgatgaa
     5221 gatggaggac ttgccctcag acctcatgcg gtgcacgacg gcggagagct cctccgcctc
     5281 ctggggcgtg aggacggccg tgggctcgtc caggaccagg atctccgcgt cgcggtacag
     5341 gagcttgagg atctccacgc gctgctgctc gccgacggag agctgccaga tgaaggcgtc
     5401 gggatcgacc ttgaggttgt agcactccga gaggcggagg atctccttct tcgccgcctt
     5461 catatcgggg acgaagggga ggccgcggag gcccaggacg acgttctcgg cgaccgtcat
     5521 cgagtccacg agcatgaagt tctggtggac catcccgatg ccgagggcgg aggcgtcgcg
     5581 cggcgactcg atctccgccc gagcgcccct gacgaggatc tcccctgagt cgggccggta
     5641 gaggcccacg aggatgttca tgagggtgct cttgcccgcg ccgttctccc cgaggagggc
     5701 gaggacctcg ccccgctcca ggcggaggtc gatgccgtcg gaggcctgga ccccggggaa
     5761 gctcttccgt atgccgcgca tctcgacgag gcgcccctcg ccgccctccc cgggggagca
     5821 ggccgcccgg gggctccgcg ccgcgcgcgg ggcgggcccg ccttctttgt ccttcatcaa
     5881 cgtggcttga aacctttcag gaggcccggg ctcgcggccc gcgggatggc gctcccctag
     5941 acaagaaaaa tcctccggcg cgccttgagg tggccgcgcc ggaggatcct ggggatccgg
     6001 cggccgcgcc gcgccttccc ccgtctccgg ggcgcgcggg cggccttccg gctcacttca
     6061 ccttgccgac gactccctgg acgaaccagt ccatggagag ctgcttgccg tcgtcgagga
     6121 tctccccctc cttgacggcc agggcgccgg actggtcctt gatcgggccg gcgaagatgt
     6181 tcatcttgcc ggaggcgatc ttctcctggg cctcggcgac gagcttcctc gtgtcctcgg
     6241 gcaccttcgc gctcatgtcg gagagggcga cgacgccggc ggagaggcct tcccagtact
     6301 ggtgggtctt ccactcgccc ttcatcgcct tgtcgatcgt gtccttgtag tagacggccc
     6361 agttccacat cgggctcgcg aggacggagt cgcccacgaa cttgcccatg tcggagtcgt
     6421 agccgatgga gagcttgccc cgctcctggg cggccttctg cggctcgacg gtgtcctggt
     6481 gctgggcgat gacgtcgcag ccggcgtcga ggagggccac ggcggcctcg cgctccttga
     6541 cggggtcgta ccaggtgttg gtccagacga cgcggacctc ggccttgggg ttgacggact
     6601 tgaggccgag ggtgaagccg ttgatgccgc ggacgacctc ggggatgggg aaggcggcga
     6661 cgtagccgat cttgttggac ttggtggcct tgccggcgat gatgccggag aggtagcggg
     6721 cctcctcgat gcggccgaag taggtggcca tgttctccgc ggtcttgtag ccggcgcagt
     6781 gctcgaactt gaccttcggg aactccttgg cgacctcgag catcgggtcc atgtagccga
     6841 aggaggtggc gaagatcatg tcgtagcctt cctgggcgta ctggcggatg acgcgggcgg
     6901 cgtcggggcc ctcggggacg ttctccatgt aggcggtctg gatcttgtcg ccgtaggtct
     6961 ccacgacggc cttgcgggcg cggtcgtgct cgtaggtcca gccgaggtct ccgggcgggc
     7021 cgatgtacac gaaggcgacc ttgaagggct tggcctccgc cgccgcgggc gccgcggcct
     7081 tcttggtgca gcccgcgagg gcgagggcga gcgacaggac ggcgacgcat gctaccgcga
     7141 atttcctctt catcgacgac tcctttctgc gattctcgtt catgaacgcg cttgtgccgg
     7201 agatgctcgg ccggacgggg ccggcccaac ggatagtaaa agccagtcgg ggcgatatgt
     7261 caaaccattt ttatcgcgat gcgcgaaaag gccgcctcct ggagggaagg cggcctttga
     7321 ttccggccag gttccggttc ggggccgggc cggggggcct ctacgaggcc ctgccgcccg
     7381 ggagggtcgg cgggacgggc gggagacttg cccggctaga gggtcctctc gacggaggtg
     7441 gtccagatgc ctgcctcttc gcagccgggc acggccttga tcctgccgag ccagccctcg
     7501 gcctcgctct tcttcgcgta ggggccgacg cggacgcggt accagctttt gccggagatg
     7561 tccttcacgg tgatgagggc ggccaggccc cgccgcccga ggctctcctt gagctcgtcg
     7621 gcgcgggcgc ggctcgtgaa gctcgcggcc tggatccagt actcgtcgac gcggaccgtc
     7681 ttcgtcgggg cggccttcgc tgggacggcc ttggcggcgg gcttggccgc cggcgcggga
     7741 gccgtggcgg caggcttggc cgccggctgg acagccgccg ccgggacact cgccgcgggg
     7801 gccgcggcgc ccgggacggc gccggggacg gcgg
//
LOCUS       DAOOSJ010000120         7827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5812_length_7827_cov_6.510165, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000120 DAOOSJ010000000
VERSION     DAOOSJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_7827_cov_6.510165"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2517
                     /locus_tag="PLG14_07145"
     CDS             <1..2517
                     /locus_tag="PLG14_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX48950.1"
                     /translation="PLVLVLQSYQRDYAWTVGIEAGLEAGFASVPAAARPAVLVEHLD
                     AKRRDDPASEAAFAAYLKERYRGTRLAAVVVCDNSALLFAAGRRQELFPGVPLVFCGI
                     NDWNPLLLKGESGATGVLERPDYAATAAAALRLLPGRRRVYLVSDATLTGRATAADCR
                     RALSALDPKLEISDVPDLPLSGLAEWAAGLPADALLFFLAYVTDGAGVPRRAEEVAAA
                     FGARLPIVTSHDVFFTPGVLGGRIATAEGQGRAAASIALRVLSGESPDSIPLADDEAN
                     EYRFSFAELKRWGIDERALPKGSVVVGKPRGFFEREPWAAPLLGSVAFLLLLLATTLV
                     VVQVSQRKAREALAASERKFRGLFQSMAFGVAHHAIVRGRDGKPADYRFIDVNGEFER
                     LLGFGRSEVVGRLASEVYGAPYYLERFASVADGADSVAFDLPFRDRVYRTTIYSPDPG
                     EFATIFEDVTEHLAARAALEESELRYRSLFDRMEEGVVYEDDEGRVVSANPAAERILG
                     LALESMEGRAPEDPRWSCVRTDGSPFPPEELYTVRALVEGRPHSGLMGIHNPDLGKTV
                     WVQASAVPEFKPGAERPFRVFTTLADVSALKEAEVRLEAQAAELAAKNAELERFTYTV
                     SHDLKSPLITIKGFLSCIAEDAASGDAELLASDLGRVSRAADRMQELLSDLLELSRIG
                     RVANPSRRFSMGEAVSQALESLGGLVASSKAEISLPDAWPEAVADEARVAEVWQNLLE
                     NAVKYSGGRPPRIVLGWERAEGELRYFARDEGIGIEPRYLGTVFGLFNKLDPKSEGTG
                     IGLALVRRIVELHGGRAWAESGGEGKGATFLFVLPEPPAA"
     gene            2568..3446
                     /locus_tag="PLG14_07150"
     CDS             2568..3446
                     /locus_tag="PLG14_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOX48951.1"
                     /translation="MPIPIYPESAPISLEMRDELYPSLNLLKDGISEFNFSNLFLFRH
                     TYGYRVSRVPGRTFVVEGSKQGKSFFYVPCAVPDRGTLAALMDSHDYLKNLSETQAEE
                     SRLELESWGYRVEEDRDNFDYLYRRTDLAELSGKEYHKKRNLVNAFLNSYSYEQKPLS
                     KANVADAVAVLDEWRAAKGYEGDYAAAREALELYDLLGMRGCVYYVDGAPAGWCLGEP
                     LSKARMFAVHFEKACDRYKGIYQFINQAFAQALPAYIRSVNREQDLGNEGLRQAKMTY
                     RPSGFVKKYRVVKEGR"
     gene            3443..4621
                     /locus_tag="PLG14_07155"
     CDS             3443..4621
                     /locus_tag="PLG14_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48952.1"
                     /translation="MSSVDPSGLAALVRPGAPGEAGFDLSVRPGLPALALAARGRGPS
                     GAPGLEAWRAELAGKTATLTLLAGAGTRWVKSLASVRARTSGAEPAALSPAEREALAF
                     DPEKPRGLFPVRDLLGAGRAFVPGAEPRVPMAAYALAAVKGLGSHLVVVRGHEAEIES
                     EILSPLGFEPGSCRFFTQEAPFGKPLGHGDAAWQCRALWKGQEYVVANFGGDANSRLT
                     ILSALLALDALASRGEEVDLLVPAALVKAPGYPVLLDAEGLPRAFGHAKLQGAGAASG
                     RRGEASWTPAPPRADGRAYTNVGLRVYRAAALLEAVERFRRDHWEEGKGYAIPGNDPE
                     GREFALDNVDAAIAASGRARLFPVARPEELRPAKAFEDLPAFEAAVAVVAAEDRAAGL
                     "
     assembly_gap    4826..4925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5025..5498
                     /locus_tag="PLG14_07160"
     CDS             5025..5498
                     /locus_tag="PLG14_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48953.1"
                     /translation="MAAQNLVSANLSAEAKERVLAHLEGIRKELDFLAAFGEKELRGL
                     FRAGTGFAPLLDKAYLVAGEHPEILPSIFPSEEFKRDYRLYKELAPIASQVEQLARSL
                     EGTMTALSSDTLMEALEVYAAVKQHRDKVPGLGVVAADMGVFFAKARRRDAAAKD"
     gene            complement(5833..7257)
                     /locus_tag="PLG14_07165"
     CDS             complement(5833..7257)
                     /locus_tag="PLG14_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOX48954.1"
                     /translation="MKNPNKRVMRHSSAAAILSAALAAVLALAACASAPPSILAGRLP
                     EGANAFQEDFAAMARYIRKTHAGFADVPLRSMEDSAYEALVQKTWDRLAGAKDELDWH
                     FEVSFFLEKLGDGHTSLDAPRDSGLPLYLWWFEDGLYAVAASAPEGAALVGKRILAMG
                     GKGIEELEGLVNAYVPADRGSLGWKRLKSPALLSSRGMLGRLGLLDAEGRALVEYEED
                     GAARKALLGTTYSNRSAEWESRGFARIPATLPKEGNSFEYLEAFEALYLQLNSLPTAV
                     DRPFLEAAFRLAKESGAKRLILDLRNNSGGNSAWCDEFLRYIVREPRNLYLYNGWESA
                     YPGPNARRADGTVRIEPVTNGLGFKGELFVLTGPRTFSSATFFAVAAKDNGLGLLVGE
                     PCGNTSIRYGFRAAPIVLPRSGKSFSTTKYVWGRAKPGVLGEGPVLIEPDLYAAPSFA
                     DFRARRDPALERIAQELSRTAAAP"
BASE COUNT         1060 a         2824 c         2709 g         1134 t
ORIGIN      
        1 cccctcgtcc tcgtcctcca gtcctaccag cgcgactacg cgtggacggt cggcatagag
       61 gccggcctgg aggcgggctt cgcctccgtc ccagccgccg cgaggccggc cgtcctcgtg
      121 gagcacctgg acgccaagcg ccgcgacgat cccgcctcgg aggcggcctt cgcagcctac
      181 ctcaaggaac gctacagggg cacgaggctc gcggccgtcg tcgtctgcga caattccgcc
      241 ctccttttcg cggccgggcg ccggcaggag cttttccccg gcgtccccct ggtcttctgc
      301 ggcatcaacg actggaatcc cctcctcctc aagggcgaga gcggagccac gggcgtcctc
      361 gagcggcccg actacgccgc gacggcggcc gcggccttgc gcctccttcc cgggcgcagg
      421 cgcgtctatc tcgtgagcga cgcgaccctc accggccggg cgacggcggc cgactgccgc
      481 agggccctct ccgccctgga tcccaagctc gagatcagcg acgttccgga cctgcccctc
      541 tccggcctcg ccgaatgggc cgccggcctc cccgccgacg ccctgctgtt cttcctcgcc
      601 tacgtgacgg acggggccgg tgtgccccgc agggccgagg aggtggccgc ggccttcggc
      661 gccaggctgc ccatcgtgac gagccacgac gtcttcttca ctccgggggt cctgggcggc
      721 aggatagcga cggcggaggg gcagggcagg gcggccgcgt ccatcgcgct ccgcgtcctc
      781 tcgggcgaga gccccgactc catacccctc gccgacgacg aggccaacga gtaccgcttc
      841 tccttcgccg agcttaagcg ctggggcatc gacgagcgcg ccctccccaa gggctcggtc
      901 gtcgtcggga agcccagggg cttcttcgaa cgcgagccct gggccgcacc cctccttggg
      961 agcgtggcct tcctcctcct cctcctcgcg acgacgctcg tcgtcgtcca ggtctcgcag
     1021 cggaaggcga gggaggccct ggccgcgagc gagaggaaat tccggggcct cttccagagc
     1081 atggccttcg gcgtcgccca tcacgccatc gtccgcgggc gggacgggaa gcccgccgac
     1141 taccgcttca tcgacgtgaa cggcgagttc gagcgcctcc tcggcttcgg gcggagcgag
     1201 gtcgtcggcc gcctggcctc ggaggtctac ggcgcgccct actacctgga gcgcttcgcc
     1261 tccgtcgcgg acggcgcgga cagcgtcgcc ttcgacctgc ccttccgcga ccgcgtctac
     1321 aggacgacga tctactcgcc cgaccccggc gagttcgcca ccatcttcga ggatgtcacc
     1381 gagcacctcg cggccagggc ggccctggag gagtcggagc tccgctatcg gagcctcttc
     1441 gaccgtatgg aggagggcgt ggtctacgag gacgacgagg ggcgggtggt ctccgccaat
     1501 cccgcggccg agcggatcct gggcctggcc ctggagagca tggagggccg cgcccccgag
     1561 gacccgcgct ggagctgcgt caggaccgac ggctcgccct ttccgccgga ggagctctac
     1621 accgtgcgcg ccctcgtcga gggccggccc cactccggac tcatgggcat ccacaacccc
     1681 gacctcggga agaccgtctg ggtgcaggcg agcgcggtgc cggaattcaa gccgggcgcc
     1741 gagcggccct tccgggtctt caccaccctg gccgacgtga gcgccctcaa ggaggccgag
     1801 gttcggctgg aggcccaggc cgccgagctc gcggccaaga acgccgagct cgagcgcttc
     1861 acctacaccg tctcccacga cctcaagagc cccctcatca ccatcaaggg cttcctctcc
     1921 tgcatcgcgg aggacgcggc ctcgggcgac gccgagctcc tggcctccga cctgggccgc
     1981 gtgtcgcgcg ccgccgaccg catgcaggag ctcctctccg acctcctcga gctctcgcgc
     2041 atcggacgcg tcgcgaaccc ttcccgccgc ttctccatgg gggaggcggt ctcccaggcc
     2101 ctggagagcc tgggcggcct cgtcgcgtcc tccaaggcgg agatctcgct cccggacgcc
     2161 tggcccgagg ccgtcgccga cgaggcgagg gtagccgaag tctggcagaa cctcctggag
     2221 aacgcggtca agtactcggg cgggaggccg ccgaggatag tgctgggctg ggagagggcg
     2281 gagggcgagc tccgctactt cgcccgggac gagggcatag gcatcgagcc gcgctacctg
     2341 ggcacggtct tcggcctctt caacaagctc gacccgaaga gcgagggcac cggcataggc
     2401 ctggcactcg tccgccgcat cgtcgagctt cacggcggcc gcgcctgggc ggagtccggg
     2461 ggcgagggga agggcgcgac cttcctcttc gtcctgcccg agcccccggc cgcctgaaaa
     2521 cgcggcgcgg cttgcctcga ggcccggcat tccgtatcat ctaggccatg cccataccca
     2581 tataccccga atccgccccc atatcgctcg agatgcgcga cgagctctat ccctccctca
     2641 acctcctcaa ggacggcatc tccgagttca acttctccaa cctcttcctc ttccgccaca
     2701 cctacggcta ccgcgtctcc cgggtgccgg gccgcacctt cgtcgtcgag ggctcgaagc
     2761 aggggaagag cttcttctac gtgccctgcg ccgtccccga ccgcgggacc ctggcggccc
     2821 tcatggactc ccacgactac ctcaagaacc tctccgagac ccaggcggag gagagccggc
     2881 tcgagctcga gtcctggggc tacagggtgg aggaggacag ggacaacttc gactacctgt
     2941 accgccggac cgacctggcg gagctctccg gcaaggagta ccacaagaag cgcaacctgg
     3001 tgaacgcctt cctcaactcc tacagctacg agcagaagcc gctgtccaag gccaacgtcg
     3061 ccgacgcggt ggcggtgctc gacgagtggc gcgcggcgaa gggctacgag ggcgactacg
     3121 ccgcggcgcg cgaggccctg gagctctacg acctcctggg gatgcgcggc tgcgtctact
     3181 acgtggacgg cgcgcccgcg ggctggtgcc tgggcgagcc cctgtccaag gcgcgcatgt
     3241 tcgcggtcca cttcgagaag gcctgcgacc gctacaaggg catctaccag ttcatcaacc
     3301 aggccttcgc ccaggccctc ccggcctaca tcaggtcggt gaaccgcgag caggacctgg
     3361 ggaacgaggg cctccggcag gcgaagatga cctaccggcc ctcgggcttc gtgaagaagt
     3421 accgcgtcgt gaaggagggg cgatgagctc cgtggatccc tccggcctcg cggccctggt
     3481 ccgtccggga gctccgggcg aggccggctt cgacctctcc gtccgccccg gccttcccgc
     3541 cctggccctg gccgcgcggg gccgcgggcc ttccggcgcc cccggcctcg aggcctggcg
     3601 agcggagctc gccgggaaga cggcgaccct caccctcctc gcgggcgccg ggacgcgttg
     3661 ggtgaaatcc ctggcctccg tccgcgcgcg tacgtccggc gcggagcccg ccgccctttc
     3721 gcccgccgag cgcgaggccc tcgccttcga cccggagaag ccgcgcggcc tcttccctgt
     3781 ccgcgacctt ttgggcgcgg ggcgcgcctt cgtccccggc gcggagccta gggtgccgat
     3841 ggcagcctac gccctggccg ccgtgaaggg cctgggctcc cacctcgtcg tcgtccgcgg
     3901 ccacgaggcg gagatcgagt cggagatcct ctctcccctc ggcttcgagc ccggctcctg
     3961 ccgcttcttc acccaggagg cgcccttcgg gaagcccctg ggccacggcg acgcggcctg
     4021 gcagtgccgc gccctgtgga agggccagga gtacgtcgtc gccaacttcg gcggcgacgc
     4081 gaactcccgc ctcacgatcc tctccgccct cctcgccctg gacgccctgg cctcgcgcgg
     4141 cgaggaggtc gacctcctcg tgcccgccgc cctggtgaag gcccccggct accccgtcct
     4201 cctcgacgcc gagggccttc cccgcgcctt cggccacgcg aagctccaag gcgccggagc
     4261 ggcctccggc cgccgcggcg aggcctcctg gacgccggcc ccgccccggg ccgacggccg
     4321 cgcctacacg aacgtcggcc tccgcgtcta ccgcgccgcc gccctcctcg aggccgtgga
     4381 gcgcttccga cgcgaccact gggaggaggg gaagggctac gccatccccg gcaacgaccc
     4441 cgagggccgc gagttcgccc tggacaacgt cgacgccgcg atagccgcct ccggccgcgc
     4501 ccgcctcttc cccgtcgccc gtcccgagga gctcaggccg gcgaaggcct tcgaggacct
     4561 gccggccttc gaggccgcgg tcgccgtcgt ggcggcggag gaccgcgcgg cgggtcttta
     4621 ggaacctggg cggacgggtc ctgcccccgc ccgcgcggcc ccgggcgccg gggtcgcctg
     4681 ggcgcttcgc gccgggcttt ctcccggggc gccggggcgc gcgtgggccg gggcacccgt
     4741 cgctccgccc aggcctctca agctgtgggt tgggtggtct ccttcacgcc gcgcgcctcg
     4801 cacgcgggga gtagccgcgc gggagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnncgccc ggaacggcgc ttgcgcgggg agaggcgggg cgcgatgctt gtaggggcgt
     4981 gggccccgcg gggcggggcg atccggctcg gatggaggcg gctcatggcg gcgcaaaacc
     5041 tggtttcggc gaatttgagc gcggaggcga aggagcgggt cctggcgcat ctcgagggga
     5101 tacggaagga gctcgacttc ctcgccgcct tcggcgagaa ggagcttcgg ggactcttcc
     5161 gcgcggggac gggcttcgcg cccctcctcg acaaggccta tctcgtggcg ggcgagcatc
     5221 cggagatcct gccctcgatc ttcccgagcg aggaattcaa gcgggactac cgcctctaca
     5281 aggagctcgc gcccatagcg agccaggtcg agcagctggc ccgcagcctg gagggcacga
     5341 tgaccgccct ctcgagcgac acgctcatgg aggccctgga ggtctacgcg gcggtgaaac
     5401 agcaccggga caaggtgccc gggctcggcg tcgtcgccgc cgacatgggc gtgttcttcg
     5461 cgaaggcgcg caggagggac gcggcggcca aggactaggc tcgggtggcg cttcgcgcaa
     5521 gggcgatgtc cttcgcgagg gccggttggt ccctggggag gggctccaag gccctgtaga
     5581 aggggagcgc ctttcgcgag aggggccgca ggcctttccg aaagggggtg cgagcccttc
     5641 ctgaaggggg cccgggccct tcgtgaaggg gtgcgaggtc cttcctgaag agggcccggg
     5701 cccttcttga aggggagcgt ggctctccac aagggggtca gagccccttc ggaagggctt
     5761 tggcccgcgc aagacgggcc cagtcctcct cccgaggggc tcctgcgctc ctttgttcgg
     5821 acttcgaagc gctcagggag cggcggcggt tctcgaaagc tcctgggcga tgcgctcgag
     5881 ggcgggatcc cgccgcgccc ggaagtccgc gaagctcggc gccgcgtaga ggtcgggctc
     5941 gatgaggacg gggccctcgc cgaggacgcc gggcttggcc ctgccccaga cgtacttggt
     6001 cgtggagaag ctcttacccg accgcgggag gacgatggga gcggccctga agccgtagcg
     6061 gatcgaggtg ttgccgcagg gctcgcccac gaggaggccg aggccgttgt ccttggccgc
     6121 gacggcgaag aaggtcgcgg agctgaaggt ccgcgggccg gtgaggacga agagctcgcc
     6181 tttgaacccg aggccgttcg tcacgggctc gatcctgacc gtgccgtcgg cgcgccgcgc
     6241 gttgggcccg gggtaggccg actcccagcc attgtagagg tagaggttcc gcggctcccg
     6301 cacgatgtag cggaggaact cgtcgcacca ggccgagttg ccgcctgagt tgttccgcag
     6361 gtcgaggatg aggcgctttg ccccgctctc cttcgccagc ctgaaggccg cctcgaggaa
     6421 tgggcggtcg accgccgtgg gaaggctgtt cagctgcagg tagagcgctt cgaaggcttc
     6481 gaggtactcg aaggagttgc cctccttcgg gagggtggcc ggaatcctcg cgaagccgcg
     6541 gctctcccac tcggcggagc ggttcgagta ggtcgtgcct aagagggcct tcctcgcggc
     6601 gccatcctcc tcgtactcga cgagggcgcg gccctcggcg tcgaggaggc cgaggcggcc
     6661 gagcatcccg cgggaggaga gcagggcggg gctcttgagg cgcttccagc ccaggctgcc
     6721 gcggtccgcg ggcacatagg cgttgaccag gccctcgagc tcttcgatgc ccttgccgcc
     6781 catcgcgagg atccgcttgc ccacgagcgc cgctccttcg ggggcgctcg ccgccacagc
     6841 atagagcccg tcctcgaacc accagaggta gaggggcagg cccgagtccc ggggcgcgtc
     6901 gaggctcgtg tgcccgtcgc ccagcttctc gaggaaaaag gatacctcga agtgccagtc
     6961 gagctcgtcc ttggctcccg cgagccggtc ccaggtcttt tggaccaggg cctcgtaggc
     7021 cgagtcctcc atcgagcgca gggggacgtc ggcgaagccc gcgtgggtct tcctgatgta
     7081 cctcgccatc gccgcgaagt cctcctggaa ggcgttcgcc ccctcgggca gcctccccgc
     7141 gaggatcgac ggcggcgccg aggcgcaggc ggcgagggct aggacggcgg cgagggcggc
     7201 cgagaggatc gcggcggctg aactgtggcg catgacgcgt ttgtttgggt ttttcatcgg
     7261 ctcgagtata tagcaagctc gccgttttcg cttaggcccg cgcgcgacca ctcgccccac
     7321 cgcaagtccc cgcgcgcagc gccggggctt tagcccggga agcggcgtgt cgctccacac
     7381 cgcggcccgc cttcctctcg gcatcccgcc tgtgactgcc cgccttgtcg caagtccccg
     7441 cgcgcagcgc cggggctcag ctcgggaagt ggcgtgtcgc tccgttcccc gcgcgccgcc
     7501 cagcggacaa ccttcgcgga acctcccctc ccgcgccttg agctccctct cctcccgcca
     7561 ggcggcgtga gaccgaccaa gggagcggag cgcctttcga cgctcggcgg agcggcgggt
     7621 ggcggagcct gggcgcgccc cggcgcggga gggcgcggag cgcccggaag cggccggggt
     7681 cgtccaggct tcgccaggac ccgccccggc gagcccccaa aaggcccgcc gccgcctcgc
     7741 tcgagaccgt gtcgcctgtc ggagttgagg agagctaaag gagcgggatg ggaggggaaa
     7801 tgtcgtagcc ctgcgcgtag tccacgc
//
LOCUS       DAOOSJ010000121         7758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5876_length_7758_cov_6.905491, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000121 DAOOSJ010000000
VERSION     DAOOSJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5876_length_7758_cov_6.905491"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..937)
                     /locus_tag="PLG14_07170"
     CDS             complement(221..937)
                     /locus_tag="PLG14_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX48955.1"
                     /translation="MSGKTTLRIARPGKGGAAGEAGAYGTAYSDYEIEAKEGMTLLDA
                     LESIRAGTEPSLRYRHSCHHGSCGTCGAVVDGKEALMCLVRLESLGPGPVLVEPLRKM
                     DAVGDLAVDPSPLFESLPPGSGYLRRSEVPHGLGREEGGGYERFEACIECGICVSACP
                     VPAGRPFSGPAALAMADRDREERPGLAEPALAFAARPEGVAACDRAFACSRACPQGVA
                     PGRRIESLRKALAAKATGRS"
     gene            complement(934..2166)
                     /locus_tag="PLG14_07175"
     CDS             complement(934..2166)
                     /locus_tag="PLG14_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX48956.1"
                     /translation="MKTEVLPLPRAAAKARAPRVCVIGGGGTGAALAYDLALRGLAVV
                     LLEKGELTSGTTGRHHGQLHSGARYALGDRAIARECYEETLVLRRIVPEAIEYNGGFF
                     IALSEEEAELAPAFIAACMEAGIPAREVPAARALELEPAMSPRLRRAVWVPDGTFDAF
                     RLPLAFFAAAKALGARVRPWTEVLGLEVSGGRVSAARVIDRSAEPFREERVEADYFVS
                     ATGAWAGRVGALAGLDIPITPAPGALLAVRGRLVDRVVSRLRPAGDGDILVPQRGLSI
                     IGSTQRVADDPEAIRPTREEARFLRGAGAELVPAFGDEAGAAPFHAVWAAARPLAGRS
                     SDDGRSISRDFAALDHEARDGLAGIATIIGGKATVLRAMAEKAADLVCAKLGVAAPCS
                     TADYALPSWREYYGGRKA"
     gene            complement(2224..2844)
                     /gene="udk"
                     /locus_tag="PLG14_07180"
     CDS             complement(2224..2844)
                     /gene="udk"
                     /locus_tag="PLG14_07180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOX48957.1"
                     /translation="MATAKIIGICGGTGSGKTTIVRKISELVSDFVFIPQDNYYKTAE
                     YISNSNITAFNWDHPDAFDNALLTEQLEKLRNGQAVDMPTYDFVHHRRREETVHVEPK
                     KLVIFEGIMIFTNKAVRDRIDLKIFVDTPDDIRFIRRLQRDIKERGRTVDSVIEQYLT
                     VVRPGHYEFIEPTKQYADIIIPEGGNNENALNVLITFINSVINAAD"
     gene            complement(2989..3552)
                     /locus_tag="PLG14_07185"
     CDS             complement(2989..3552)
                     /locus_tag="PLG14_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX48958.1"
                     /translation="MDHLPELAARRAYRSISAEPLSGPEVERLLEAATLAPSCFNNQP
                     WRLVAARGEALDKVREALTEGNRWATRAPLVVVLATKPSLDCRQDEGRDYAYFDTGLA
                     AMALMLEATRMGLYAHPIAGYSPAKVRKAVGLPPDFVPLALVVVGKPGDSSLLAEWQK
                     EREAGPRQRKPLSEVAFDGTWEKPWGA"
     gene            3683..4984
                     /locus_tag="PLG14_07190"
     CDS             3683..4984
                     /locus_tag="PLG14_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX48959.1"
                     /translation="MKRIASIALLAALVAAPLFAQAPVVPELKGKNVEITIWTHEDAN
                     RAPLEKGYIEQFTAANPGVKVNYVTYPSSKIREIVTTGFAANQGPTVFNLELFDEYPF
                     ITNGRVAPVDYKMIGVKSQKDLEAKYLPGMISPVVDGGKVYGLPLELTNWCVYLNKKI
                     FRDAGLDPDKDWPKTWEDVMAVSEKIVQRNGQIITRRGFDFRYPYYLTSVVPMVEQLG
                     GELVAKDGKKAIVGDAAWLKVLSYFRDFGPNGKNLGSPTYTAARNLFDLDKNEVAMSL
                     SGLYQEARMKGKNPAFYESKDWMVIPYPQWKDAKKKVPNNYYGHYYMVNAQSAKLEQQ
                     AAWALLGYMLSHGEEYLSKVAVVQPTKALMDSATFKGMPYSDVFAKDMAAAHMVYYGA
                     SSSKIDASLKEAFESVMLAGVEPQKALDKLRKDVTAILEEE"
     gene            5072..5983
                     /locus_tag="PLG14_07195"
     CDS             5072..5983
                     /locus_tag="PLG14_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOX48960.1"
                     /translation="MKGKPTVRSLEKGRARWGWAFVAPGILFFSIFSFYPILNAFYTS
                     FFNKKLLSLKAPKFIGFQNYERVLSSPDFWNSVRATAVFAAGAFVPLFLLSLLFAVLI
                     VSRKRFQRTAQMALYSPAVLSSAVAALIWLLIFDPRGLANQFVNLVANTPGIDHKWLA
                     DATMVQLSTILVYVWKYVGYFTILFVTGIGKIPQSVNEAAVIDGAGPVQRFFRITLPL
                     LRPTTVLVSVMILIQCLKTFSTQYLFTQRGAPLGPINVLTLNIYNTALRDQNLGRASV
                     MSVILFLCMLLLSWIQIRFSRSEEAEL"
     gene            5989..6849
                     /locus_tag="PLG14_07200"
     CDS             5989..6849
                     /locus_tag="PLG14_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOX48961.1"
                     /translation="MTRAGRSRWKSLPGAVLAVAVLSAFSLLILVPLLFMAGASLMPP
                     HEIMKMPYDWIPKEWKWANFHQALAGNDGNWIYLRNIFNSLVVSLTIAFTTVLLSASV
                     GYGLAKFDFKGRNAVFMGIMATMMIPFETVMIPLYMIAVKLGMQDSYAGLIVPFMMNA
                     FGVFLMRQYLLTFPSEILDAARIDGCSEPRIFREIVFKNSGPAAATLAILAFRQQWDN
                     LLWPLLIAQREEMKTIPAYIVKFAAEKNADEGAMMAVAAIASIPVFALFFGLSKYFLG
                     GSAVYSAGKE"
     gene            6846..7472
                     /locus_tag="PLG14_07205"
     CDS             6846..7472
                     /locus_tag="PLG14_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOX48962.1"
                     /translation="MIELYIFDQGGVLSRDFDIGPEAARRLGLALPEFRALAAPDLMP
                     FMRGEIDSAEFWRRFEARSGLRAAEDYWKTLFRPRLDPETVALVRELSRGARTVSGTN
                     TIAVHHEINLALGHHDAVQAVYASHLMGFAKPEPEFWLAILEAEGVAPGRAFFVDDYP
                     ENVEAARSLGLEARLYVSAAELRRELCALGAPVSPAPRPAAAEPARLQ"
     gene            7484..>7758
                     /locus_tag="PLG14_07210"
     CDS             7484..>7758
                     /locus_tag="PLG14_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48963.1"
                     /translation="MNGRRAAAAVLLAAASLGLSSCARPAAAATEAGEADLVVYSSHP
                     EELVRVVVAEFRDRTGLSVRLVQAGTGELLRRLRSEADSGRAPGESC"
BASE COUNT         1168 a         2752 c         2610 g         1228 t
ORIGIN      
        1 aaggaacgag gccgccgacg ttggactgca aggaatagta ttgtcgtcgc cgcgtcccgc
       61 ggcgcgcgta tcaacgtcgg cggctccgcc gtgacgaact gcgtgacggt cctgaggcgg
      121 acgcgctaga aggaacgagg ccgccgacgt tggactgcaa ggaatagtat tgtcgtcgcc
      181 gcgttccgcg gcgtgagtgg cgcgccggag ccgccggggc ctagctccga cctgtagcct
      241 tcgcagccag cgccttcctg aggctctcga tgcgccttcc cggcgcgacg ccctgggggc
      301 aggcacgcga gcaggcgaag gcccggtcgc aggcggcgac gccctcgggc ctggccgcga
      361 aggccagggc gggctcggcg aggccggggc gctcctcgcg gtcgcggtcg gccatcgcga
      421 gggcggcggg gccggagaag gggcggccgg caggcaccgg acaggccgag acacagatgc
      481 cgcactcgat gcaggcctcg aagcgctcgt agccgccgcc ctcctcgcgg ccaaggccgt
      541 gggggacctc gctccgcctg aggtagcccg agccgggcgg cagcgactcg aagaggggcg
      601 aggggtcgac ggcgaggtcg cccaccgcgt ccatcttccg caggggctcg acgaggacgg
      661 ggccgggacc gagggactcg aggcgcacga ggcacatgag cgcctccttc ccgtccacga
      721 ccgcgccgca ggttccgcag gagccgtggt ggcaggagtg ccggtagcgg agcgagggct
      781 ccgttccggc ccgtatcgac tccagggcgt cgaggagggt catgccctcc ttcgcctcga
      841 tctcgtagtc ggagtaggcc gtcccgtacg cgccggcctc gcctgccgct ccgcccttcc
      901 ccgggcgcgc gatccgcagc gtcgtcttcc cgctcatgcc ttcctcccgc cgtagtactc
      961 ccgccagctc ggcagcgcgt agtccgcggt agagcagggt gcggcgacgc cgagcttcgc
     1021 gcagaccagg tcggcggcct tctccgccat cgcgcgcagg accgtcgcct tgccgccgat
     1081 gatcgtggcg atgccggcca atccgtcgcg cgcctcgtgg tccagggcgg cgaagtcccg
     1141 gctgatggac ctaccgtcgt cggaggagcg cccggcaagg ggccgcgcgg cggcccagac
     1201 ggcgtggaag ggcgctgccc ccgcctcgtc gccgaaggcg gggacgagct cggcgccggc
     1261 gccgcgcagg aagcgggcct cctcgcgggt cggccggatc gcctcgggat cgtcggcgac
     1321 gcgctgcgtc gagccgatga tggagaggcc gcgctggggc accaggatgt cgccgtcgcc
     1381 ggcgggccgg aggcggctca cgacgcggtc cacgaggcgg ccgcggacgg cgaggagggc
     1441 gccgggcgcc ggcgtgatgg ggatgtcgag gccggccagg gcgccgaccc tgcccgccca
     1501 ggcgcccgtc gcgctcacga agtagtccgc ctcgacccgc tcctcgcgga agggctcggc
     1561 gctccggtcg atgacgcggg ccgcggagac gcggcctccc gagacctcga ggccgaggac
     1621 ctccgtccag ggacgcacgc gcgctccgag ggccttcgcg gcggcgaaga aggcgagggg
     1681 gaggcggaag gcgtcgaagg tcccgtcggg gacccagacc gcgcgcctca agcgagggct
     1741 catcgcgggc tcgagctcga gggctcgggc ggccgggact tcccgggccg ggatgcccgc
     1801 ctccatacag gccgcgatga aggccggcgc gagctcggcc tcctcctcgg agagggcgat
     1861 gaagaagccg ccgttgtact cgatggcctc ggggacgatg cggcggagga cgagggtctc
     1921 ctcgtagcac tccctcgcga tggctcggtc gcccagggcg tagcgcgcgc ccgagtgcag
     1981 ttggccgtgg tggcggcccg tcgtgcccga ggtgagctcc cccttctcaa ggaggacgac
     2041 ggcgaggccc cgcagggcca ggtcgtaggc gagggcggcc cccgtgcctc cgccgcctat
     2101 cacgcagacc ctgggggccc gcgccttggc ggcggcgcgg gggagaggca gcacttctgt
     2161 cttcacggag cggagtatag gcgaggggag gggagggggc aagcgctttc ggcgcgggcg
     2221 cccctagtcc gcggcgttga tgaccgagtt gatgaaggtg ataaggacgt tgagggcgtt
     2281 ctcgttgttg ccgccctcgg ggatgatgat gtcggcgtac tgcttggtcg gctcgatgaa
     2341 ctcgtagtgg ccggggcgca cgaccgtgag gtactgctcg atcacggagt ccacggtgcg
     2401 gccgcgctcc ttgatgtcgc gctggaggcg gcggatgaag cggatgtcgt ccggcgtgtc
     2461 gacgaagatc ttgaggtcga tgcggtcccg cacggccttg ttcgtgaaga tcatgatgcc
     2521 ctcgaagatg acgagcttct tgggctcgac gtggacggtc tcctcgcggc ggcggtggtg
     2581 gacgaagtcg taggtcggca tgtccacggc ctggccgttc cggagcttct ccagctgctc
     2641 ggtgaggagg gcgttgtcga aggcgtcggg atggtcccag ttgaaggcgg tgatgttgga
     2701 gttggagatg tactcggcgg tcttgtagta gttgtcctgg gggatgaaga cgaagtcgga
     2761 gacgagctcg gagatcttgc ggacgatggt ggtcttgccc gagcccgtac cgccgcagat
     2821 gccgattatt ttcgccgtag ccatggaacc ttcccgtccc gcggccgcgg gacggtgggg
     2881 atcatagcac ggcggccgaa gcttgaaaag cgcggggagt ccggacgcca agctttggcg
     2941 cgggccgccg ggctcccctg ccaaggcccg ggcggcgtac gcccccgctc aggcccccca
     3001 gggcttctcc caggtcccgt cgaaggctac ctcggagagg ggcttgcgct gcctcgggcc
     3061 ggcttccctc tccttctgcc actccgcgag gagggagctg tcgcccggct tgccgacgac
     3121 gaccagggcc agggggacga agtcgggggg gaggccgacg gccttcctga ccttggccgg
     3181 ggagtagccg gctatggggt gggcgtagag gcccatccgg gtcgcctcca gcatcagggc
     3241 catggccgcg aggcccgtgt cgaagtaggc gtagtcccgc ccctcgtcct ggcggcagtc
     3301 cagggagggc ttggtggcga ggacgacgac gaggggggcc cgggtggccc agcggttgcc
     3361 ctccgtgagg gcctccctca ccttgtccag ggcctcgccg cgggccgcga cgaggcgcca
     3421 gggctggttg ttgaagcagg aaggcgcgag ggtggcggcc tcgaggaggc gctcgacctc
     3481 cgggccggag aggggctcgg cggagatcga gcggtaggcg cggcgggcgg cgagctcggg
     3541 aaggtggtcc atgtcagcct ccatccgcta agatagcctt cccggccgga ttcgtccaca
     3601 aaatgcccgg atcgccgcat tgcgcgtccg cggaaaggga tcgaagcttg taggcgacca
     3661 atcatccaat aggaggaatc ccatgaagag aatcgcgtcc atcgcgctcc tcgcggccct
     3721 ggtcgccgcc ccgctcttcg cgcaggctcc cgtcgtgccc gagctcaagg gcaagaacgt
     3781 cgagatcacc atctggaccc acgaggacgc gaaccgcgct cccctggaga agggctacat
     3841 cgagcagttc acggccgcca accccggcgt gaaggtcaac tacgtcacct acccctcctc
     3901 gaagatccgc gagatcgtca cgacgggctt cgccgccaac cagggcccga ccgtcttcaa
     3961 cctcgagctc ttcgacgagt accccttcat cacgaacggc cgcgtcgcgc ccgtggacta
     4021 caagatgatc ggcgtgaaga gccagaagga cctcgaggcc aagtacctgc ccggcatgat
     4081 ctcccccgtc gtcgacggcg gcaaggtcta cggcctcccc ctcgagctca cgaactggtg
     4141 cgtctacctc aacaagaaga tcttccgcga cgcgggcctg gaccccgaca aggactggcc
     4201 caagacctgg gaggacgtga tggcggtctc cgagaagatc gtccagcgca acggccagat
     4261 catcacccgc cgcggcttcg acttccgcta tccctactac ctcacctcgg tcgtgccgat
     4321 ggtcgagcag ctgggcggcg agctcgtcgc gaaggacggc aagaaggcca tcgtcggcga
     4381 cgcggcctgg ctcaaggtcc tctcctactt ccgcgacttc ggcccgaacg gcaagaacct
     4441 cggctccccg acctacaccg ccgcgcgcaa cctcttcgac ctggacaaga acgaggtcgc
     4501 catgtccctc tccggcctct accaggaggc gcggatgaag ggcaagaacc ccgccttcta
     4561 cgagtccaag gactggatgg tcatccccta cccccagtgg aaggacgcca agaagaaggt
     4621 cccgaacaac tactacggcc actactacat ggtcaacgcg cagtcggcca agctcgagca
     4681 gcaggccgcc tgggccctcc tcggctacat gctctcccac ggcgaggagt acctctccaa
     4741 ggtcgccgtc gtccagccca ccaaggccct catggactcc gcgaccttca agggcatgcc
     4801 ctactccgac gtcttcgcga aggacatggc cgcggcccac atggtctact acggcgcctc
     4861 cagctccaag atcgacgcct ccctcaagga ggccttcgag tccgtgatgc tcgccggcgt
     4921 cgagccccag aaggccctcg acaagctcag gaaggacgtc accgccatcc tcgaggagga
     4981 gtgatccccc ttccctgacc gtctgggggc ggccccttcc ggggccgccc cctttcccga
     5041 ccgacccgga ttcacgtttc ggaggatgac cttgaaaggc aagccaaccg tccgctccct
     5101 cgagaaaggc cgcgcccgct ggggctgggc cttcgtggcg ccgggcatac tcttcttctc
     5161 gatcttctcg ttctatccga tcctcaacgc cttctacacg agcttcttca acaagaagct
     5221 cctctccctc aaggcgccga aattcatcgg cttccagaac tacgagcggg tgctctcgtc
     5281 gcccgacttc tggaactccg tgcgcgcgac cgccgtcttc gccgccggcg ccttcgtgcc
     5341 cctcttcctc ctctccctcc tcttcgccgt cctcatcgtc tccaggaagc gcttccagcg
     5401 cacggcgcag atggccctct actcgcccgc ggtcctctcc tccgcggtcg cggccctgat
     5461 ctggctcctc atcttcgacc cgcggggcct cgcgaaccag ttcgtcaacc tcgtcgcgaa
     5521 cacgccgggc atcgaccaca agtggctggc cgacgcgacg atggtccagc tctccacgat
     5581 cctcgtctac gtgtggaagt acgtcggcta cttcacgatc ctcttcgtga ccggcatagg
     5641 caagataccc cagagcgtga acgaggcggc cgtcatcgac ggggcgggcc ccgtccagcg
     5701 cttcttccgc ataaccctgc cgctgctccg gccgaccacg gtcctcgtgt cggtgatgat
     5761 cctgatccag tgcctcaaga ccttctccac ccagtacctc ttcacccagc gaggcgcccc
     5821 cttgggaccc atcaacgtcc tcaccctgaa catctacaac accgccctgc gcgaccagaa
     5881 cctgggccgg gccagcgtga tgagcgtcat cctcttcctc tgcatgctcc tcctctcctg
     5941 gatccagatc cgcttctccc ggagcgagga ggccgagctg tgagcgccat gacccgggcg
     6001 ggccggtccc gctggaagtc cctgcctggc gcggtcctgg ccgtcgccgt cctctccgcc
     6061 ttctccctcc tcatcctcgt gcccctcctc ttcatggccg gcgcctccct catgccgccg
     6121 cacgagatca tgaagatgcc ctacgactgg atcccgaagg agtggaagtg ggccaacttc
     6181 caccaggccc tggcgggcaa cgacgggaac tggatctatc tcaggaacat cttcaactcc
     6241 ctcgtcgtat ccctcacgat agccttcacg accgtcctcc tctcggcctc ggtcggctac
     6301 ggcctcgcga agttcgactt caagggcagg aacgcggtct tcatgggcat catggccacg
     6361 atgatgatcc ccttcgagac ggtgatgatc ccgctctaca tgatcgccgt gaagctcggg
     6421 atgcaggact cctacgcggg cctcatcgtg cccttcatga tgaacgcctt cggcgtcttc
     6481 ctcatgcggc agtacctcct gaccttcccc tcggagatcc tggacgccgc ccgcatcgac
     6541 ggctgctcgg agccgcgcat cttccgcgag atcgtcttca agaactccgg gcccgccgcg
     6601 gcgacgctcg cgatcctggc cttccgccag cagtgggaca acctgctctg gcccctcctc
     6661 atcgcccagc gcgaggagat gaagaccatc cccgcctaca tcgtgaagtt cgccgcggag
     6721 aagaacgcgg acgagggcgc gatgatggcg gtcgcggcga tagcctcgat cccggtcttc
     6781 gcgctgttct tcggcctgtc caagtacttc ctgggaggct cggcggtcta ttccgcgggc
     6841 aaggaatgat cgagctctac atcttcgacc agggcggggt cctgtcccgc gacttcgaca
     6901 tcgggcccga ggccgcgagg cgcctcgggc tggccctgcc cgagttcagg gccctggccg
     6961 cccccgacct catgcccttc atgcggggcg agatcgactc cgcggagttc tggcgccgct
     7021 tcgaggcgcg ctcgggcttg cgcgccgccg aggactactg gaagaccctg ttcaggcccc
     7081 gcctggaccc ggagacggtc gcgctcgtcc gcgagctctc ccgcggcgcg aggacggtga
     7141 gcggcacgaa cacgatcgcc gtccaccacg agatcaacct ggccttgggc caccatgacg
     7201 ccgtccaggc cgtctacgcc tcccacctca tgggcttcgc gaagcccgag ccggagttct
     7261 ggctcgcgat cctcgaggcc gagggcgtcg cgcccgggcg ggccttcttc gtcgacgact
     7321 acccggagaa cgtcgaggcc gcgcggtccc tgggcctgga agcgcggctc tacgtctccg
     7381 ccgccgagct ccggcgcgag ctttgcgccc tgggcgcgcc ggtgtcgccg gccccccggc
     7441 cggcggcggc cgagcccgcg aggctccagt gagccgagcc gccttgaacg gccggagggc
     7501 cgcggcggcc gtcctgctcg ccgcggcttc cctcggcctc tcctcctgcg cccgccccgc
     7561 cgcggcagcg accgaggcgg gggaggccga cctcgtcgtc tacagttccc atcccgagga
     7621 gctcgtccgc gtcgtcgtcg ccgagttccg cgaccggacg gggctctccg tccgcctggt
     7681 ccaggccggg accggcgaac tccttcggcg cctccgctcc gaggccgatt cgggccgcgc
     7741 gcccggcgag tcctgcga
//
LOCUS       DAOOSJ010000122         7756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5878_length_7756_cov_5.821841, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000122 DAOOSJ010000000
VERSION     DAOOSJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5878_length_7756_cov_5.821841"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..2341)
                     /locus_tag="PLG14_07215"
     CDS             complement(221..2341)
                     /locus_tag="PLG14_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX48964.1"
                     /translation="MKLSTKLFLLVAAPLALAAGLGVVAGVSGSAYLREVSKGKLAAA
                     AFQASSSLVSALQRERGATNVFLLGGEPPEELSALAAASDEALAAWKPAASASGLDAE
                     GGFAGAMSALPALRESVRSGAARPEEAIKAYSGLIGVLIDFEKLAAEASNAESAGRLS
                     SVLILEEAKEAAGRTRALAAGAAAADRPLGAESLGELVTNFGALRSFLESRGVQVSSA
                     SSVEIGVIFRSDPYIELTGIVLGLVGKADRGGYGIDPARAFAAASAVMDQIQASIARE
                     LAFASDALAAEGARVRDGLVATVAAGLAVLLAIAASALLILKRITRRISAIGSAFSRI
                     AEGEGDLTRSVEADSADELGELARDYNAFAASLRGTIASVKAAALSLRGDMDGLSDNM
                     GRTASAVEEIAATIDSIKQQSLSQSAGVTETSSTVEEMARRVEVLGGAIERQSESLSV
                     SSSSVEELIANVQSVTASIERMGENYVRLMEKSGKGRGSIAEAASKAKEIDEQSESLQ
                     EANALISGIAAQTNLLAMNAAIEAAHAGEAGRGFAVVADEIRKLAENAAAQSKNVARN
                     IASIRGVIGEVVSSSGQAERGFGEIYDQIESLSHLEEEIKFAMQEQSAGSAQILEALA
                     SMNGVTREVRSESEAMREGAAAILDETRRLLRITAELDQGMNEMAAGADQIRTAAAST
                     NELALRSADSVRALAAGMEQFKTE"
     gene            complement(2345..3022)
                     /locus_tag="PLG14_07220"
     CDS             complement(2345..3022)
                     /locus_tag="PLG14_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48965.1"
                     /translation="MRSAERAAAEAARLAAARAKRAARAGAYDALFAPWTLSAAGFLV
                     SIAFLFQPVLGLKALLFAAFLAAALASGKKVSLAATVFVSLGIVAANLLVPVGKVLWR
                     LGPFLVTETALWDGVGKAVTFEGLVYVSKATIRPGLRLPGRFGSIVARAFVYYDRIIE
                     YRGRLKPATLFEDADALMLAVWDDAEAPEAPPPEARPPRGALGYVLAAIAVAASYAAL
                     AAGILGR"
     gene            complement(3015..3626)
                     /locus_tag="PLG14_07225"
     CDS             complement(3015..3626)
                     /locus_tag="PLG14_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOX48966.1"
                     /translation="MMLSTPAPTKAPKAPESPEAAGRAEVRTGLVRPDKADVTALLGA
                     FCFFLSAIEYMIPKPLPFMRLGIANLPILLAADLLPLPWFLVLALVKVVGMSVISGSL
                     FSYIALFSLAGTLVAALVMWAAKRAGGRRISAVGVSVLGAVASNAVQVVLARAVVFGD
                     AARLIAPVFLGMGLVTGALLGLFAERFTATSAWYARASGRSGA"
     gene            complement(3598..4002)
                     /locus_tag="PLG14_07230"
     CDS             complement(3598..4002)
                     /locus_tag="PLG14_07230"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOX48967.1"
                     /translation="MKARKAASSMRLRLKPLDWPILVACAALVAFSAASVYGGGGSEL
                     RVVINGEGGEEWVYPLDKDREIPVQGPLGTTLVEIHEGHVHIADSPCPNKTCVAAGSV
                     SLPGQWVACLPNSVFVRVEGFSSDDAVDAGAY"
     gene            complement(3999..4544)
                     /locus_tag="PLG14_07235"
     CDS             complement(3999..4544)
                     /locus_tag="PLG14_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX48968.1"
                     /translation="MIVEKDRVVSIDYSLKDDGGNLIDSSESSEPLVYLHGNGNIIPG
                     LEKQLAGKKAGDRVNCVIDPAEGYGEHDESLVFKVAKSEFDSADVEVGMQFEAHGEDG
                     AQIVTVVAVEGDEVTIDANHPLAGEKLHFDVKVVEVREASAEELEHGHVHGGCSCGDE
                     CECGDEGEEGCSCGGGCGCGN"
     gene            4806..5975
                     /locus_tag="PLG14_07240"
     CDS             4806..5975
                     /locus_tag="PLG14_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48969.1"
                     /translation="MKAKRSVIEGLASILLLALVFGLMGSAMGPENLINTIMKTAYDL
                     LINTVLYLVAITVIVGALSAFLVEFGVVRIVELLLAPFMKPLYRLPGVASLGGLLCFL
                     SDNPAILTLSRDKGYQSYFKKYQLYSLTNFGTSFGMGLIVVTYMIGQGHLNPAGGGFY
                     AEALIGVAGAVFGSVVSTRLMQASIRKRFGEEEVVPASEKESFDEDKVIARKRESVFI
                     RFLNAMLDGGKSGLDAGVSIIPGVLVISTAVMMLTWGPKDPAVGYQGLAYEGVPLLPA
                     LGRALGFLFTALFGFTSPEAVAFPVTSLGAVGAALGLVPRYLKEGLIGGNDIAVFTAM
                     GMCWSGFLSTHTGMMDGLGRRDLIGKAIGSHAVGGLAAGVFAHYLFLAFRALGAL"
     gene            6010..6807
                     /locus_tag="PLG14_07245"
     CDS             6010..6807
                     /locus_tag="PLG14_07245"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOX48970.1"
                     /translation="MSLAQAIILGLVQGLAEFLPVSSSGHLVLFKDILGLSELPLLYD
                     VILHLATLLAILLVFRRRVLGILSSLGRWAAKRNGREDAANLALVGPLLVATVVTAAI
                     GFAIQEFLPAEGVRLVSIELLVSAAVLAASALLKPGSKGYADIGVGEGLFLGVAQGLG
                     VFSGISRSGMTITAGLAAGMRREEAGEFSFLLAIPAILGAFILELKDLGSLQASVEPL
                     PLAVSALVAFLSGLLALSLLMRVVKRGKLAWFAAYLVPLGLAGLFLL"
     gene            6912..>7756
                     /locus_tag="PLG14_07250"
     CDS             6912..>7756
                     /locus_tag="PLG14_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48971.1"
                     /translation="MNDSPRSTAASLVLGSLLATAALYLLAGLLLSAGGRAGPALLLL
                     PGERLFSVFSWAAFYAPLWLAAAAAIAFVPGFRPAALFSLAASLAPFLLLAAFARLAA
                     DPAGAFAAYPLLEALGLPALGFAALLATLFLCLLIARLALLFPAGGSGGSESGEGEAG
                     DDRPGGYPRLLGAPAGFGSAAADPSALAEAGGGSETASGRGPFRLGRAGQAGRAGARG
                     ASRGLAGVDAQAGQDSILEGQDGYSFRVPDLPPPPRLRSLGEAADEDDDPPEAALEPL
                     DPLDPL"
BASE COUNT         1035 a         2808 c         2713 g         1200 t
ORIGIN      
        1 cgcggcgccg tcaggtctcc cgccgcgtcg ggcgccgcct gccggcgcgc cgggcggccg
       61 cgcgccgcct agggctgccg cgcgcggcgc ggcgccacgg cccggcagtc cgcgccgctc
      121 ggcccaagga cgcggcgccg cggtccgcgc cggccgcgcg ttcgcccggc cgtgcggcgc
      181 cgttcgtccc aaggacgcgg cgccaagccc ggtccgcggc ctactcggtc ttgaactgct
      241 ccatgcccgc cgcgagggcg cgcacggagt cggccgagcg cagggcgagc tcgttcgtgc
      301 tcgccgcggc cgtcctgatc tggtcggcgc cggccgccat ctcgttcatg ccctggtcga
      361 gctcggcggt gatcctgagg aggcgcctcg tctcgtcaag gatggcggcc gcgccctcgc
      421 gcatggcctc cgactccgag cggacctcgc gggtgacgcc gttcatggaa gcgagggcct
      481 cgaggatctg ggccgagccc gcgctctgct cctgcatcgc gaacttgatc tcctcctcca
      541 ggtgggagag gctctcgatc tggtcgtaga tctcgccgaa gccgcgctcg gcctggccgg
      601 aggaggagac gacctcgccg atgacgccgc ggatggaggc gatgttcctg gcgacgttct
      661 tggactgggc cgccgcgttc tccgcgagct tcctgatctc gtcggcgacg accgcgaagc
      721 cgcggcccgc ctccccggcg tgggcggcct cgatggccgc gttcatggcg aggaggttcg
      781 tctgcgcggc tatgcccgag atcagggcgt tggcctcctg gagcgactcg gactgctcgt
      841 cgatctcctt cgccttggag gcggcctccg ctatcgagcc gcggcccttg ccgctcttct
      901 ccatgaggcg gacatagttc tcgcccatgc gctcgatgct cgccgtgacg gactggacgt
      961 tggcgatgag ctcctccacg gaggagctgg agacggagag gctctccgac tggcgctcga
     1021 tggcgccgcc caggacctcg acgcgcctgg ccatctcctc cacggtggag gaggtctccg
     1081 tgacgccggc gctctgggag agcgactgct gcttgatgga gtcgatggtc gccgcgatct
     1141 cctccacggc gctcgcggtg cggcccatgt tgtcggagag gccgtccatg tcgccgcgca
     1201 gggacagggc cgcggccttg acggaggcta tcgtcccgcg gagcgaggcg gcgaaggcgt
     1261 tgtagtcgcg ggcgagctcg ccgagctcgt cggcggagtc ggcctccacc gagcgggtga
     1321 ggtcgccctc gccctccgcg atccgggaga aggcggagcc gatcgcggag atcctccgcg
     1381 ttatgcgctt gaggatgagg agggcggagg cggcgatggc gaggaggacg gccaggccgg
     1441 ccgcgaccgt cgcgacgagg ccgtcgcgga cgcgcgcgcc ctccgcggcc agggcgtcgg
     1501 aggcgaaggc gagctcgcgg gcgatggagg cctggatctg gtccatcacg gcggaggccg
     1561 cggcgaaggc cctcgcgggg tcgatcccgt agccgcccct gtccgccttg ccgacgaggc
     1621 cgaggacgat cccggtcagc tcgatgtagg ggtcgctccg gaagatgacg ccgatctcca
     1681 ccgagctcgc gctggagacc tggactcccc tcgactcgag gaagctacgc agggcgccga
     1741 agttggtgac gagctcgccc aggctctccg cgcccaaggg ccggtcggcg gccgcggcgc
     1801 cggccgcgag ggcccgcgtc ctgcccgcgg cctccttggc ctcctccagg atgaggacgg
     1861 aggagagcct gccggccgac tctgcgtttg aggcctccgc ggcgagcttc tcgaagtcga
     1921 tgaggacgcc gatgaggcct gagtaggcct tgatggcttc ctcggggcgc gcggcgccgc
     1981 tccttacgct ctcgcgcagg gcgggcaggg cggacatcgc gccggcgaag cctccctcgg
     2041 cgtcgagccc cgaggccgag gccgcgggct tccaggcggc cagggcctcg tcgctcgcgg
     2101 cggccagggc ggagagctcc tcgggcggct cccccccgag gaggaagacg ttggtcgccc
     2161 cccgctcccg ctggagggcg gagacgaggg agctcgaggc ctggaaggcc gcggcggcga
     2221 gcttcccctt ggagacctcg cggaggtagg cggagccgga gacgccggcg acgacgccga
     2281 gtccggcggc cagcgcgagc ggcgcggcga cgaggaggaa gagcttggtg ctgagcttca
     2341 cggtctatct cccgaggatg cccgcggcga gcgccgcgta ggaggctgcg acggcgatgg
     2401 cggcgaggac gtagccgagg gcgccgcgcg gaggcctggc ctcgggcggc ggcgcctcgg
     2461 gcgcctcggc atcgtcccag acggcgagca tgagcgcgtc cgcgtcctcg aagagggtcg
     2521 cgggcttgag cctgccccgg tattcgatga tccggtcgta gtagacgaag gcgcgggcga
     2581 cgatggagcc gaagcggccg ggaagcctga gtccgggcct gatggtcgcc ttggagacgt
     2641 agacgaggcc ctcgaaggtg accgccttcc cgacgccgtc ccagagggct gtctcggtga
     2701 cgaggaaggg gccgaggcgc cagagcacct tgccgacggg gacgaggagg ttcgcggcga
     2761 cgatgcccag ggagacgaag accgtggcgg cgagggagac cttcttgccc gaggccaggg
     2821 cggccgcgag gaaggccgcg aagaggaggg ccttgagccc gaggacgggc tggaagagga
     2881 aggctatgga gacaaggaag ccggctgccg agagggtcca gggcgcgaag agcgcgtcgt
     2941 aggcgccggc cctggcggcc cgcttggccc gcgcggcggc caggcgcgcg gcctcggcgg
     3001 cggcgcgctc cgcgctacgc accgctcctc cccgaggccc gcgcgtacca ggccgaggtc
     3061 gcggtgaagc gctccgcgaa gaggccgagg agggcgcccg tcacgaggcc catgcccagg
     3121 aagaccgggg cgatgaggcg ggcggcgtcg ccgaagacca cggccctggc caggaccacc
     3181 tggacggcgt tggaggcgac ggcgccgagg acggagacgc ccacggccga gatgcgcctc
     3241 ccccccgcgc gcttggcggc ccacatgacc agggccgcga ccagggtgcc cgcgagggag
     3301 aagagggcga tgtaggagaa gagggagccc gagatcacgc tcatcccgac gaccttgacg
     3361 agggccagga cgaggaacca gggcaggggc aggaggtcgg ccgcgaggag gatcggaagg
     3421 ttggctatcc cgaggcgcat gaagggaagg ggcttcggga tcatgtactc gatcgcggag
     3481 aggaagaagc agaaggcgcc caggagggcg gtgacgtcgg ccttgtcggg gcggacaagg
     3541 cccgtccgga cttccgcgcg gccggcggcc tcggggctct ccggcgcctt cggagcctta
     3601 gtaggcgccg gcgtcgacag catcatcgct tgagaatcct tccaccctga cgaacacgct
     3661 gttgggcagg caggccaccc actggccggg cagggacacc gagccggccg ccacgcaggt
     3721 cttgttcggg cagggcgagt cggctatgtg cacgtggccc tcgtggatct ccacgagggt
     3781 cgtgccgagg ggcccctgga cggggatctc gcggtccttg tccaggggat agacccattc
     3841 ctcgccgccc tccccgttga tcaccacgcg gagctcggag ccgccgccgc cgtagacgga
     3901 ggcggcggag aaggcgacca gggcggcgca ggcgaccagg atcggccaat cgaggggctt
     3961 gaggcgcagg cgcatggagg aggcggcttt ccgggctttc aattcccgca gccgcaaccg
     4021 cctccgcagg agcatccctc ttcgccctcg tcgccgcact cgcactcgtc gccgcaggag
     4081 cagccgccgt ggacgtgccc gtgctcgagc tcctcggcgc tggcctcgcg gacctcgacg
     4141 accttgacgt cgaagtggag cttctcgccg gcgagggggt ggttggcgtc gatggtgacc
     4201 tcgtcgccct cgacggcgac gacggtgacg atctgggctc cgtcctcgcc gtgggcctcg
     4261 aattgcatgc caacctcgac gtcggcgctg tcgaactcgg acttggcgac cttgaatacg
     4321 aggctctcgt cgtgctcgcc atagccctcc gcggggtcga tgacgcagtt cacgcggtcg
     4381 ccggccttct tgccggcgag ctgcttctcc aggccgggga tgatgttccc gttgccgtgg
     4441 aggtagacga ggggctccga gctctcggag gagtcgatca ggttcccgcc gtcgtccttg
     4501 aggctataat cgatgctgac cacgcggtct ttctcaacaa tcatgataat cctttcgagt
     4561 tgaacgctat agcctcatag tacatggcga gcgcagactc ggcaagtgga gataatgaat
     4621 atataagtaa aacgctcata aaaagcaagt gggtggcgcg ccgtcctcgg cggcgaggga
     4681 gaggaccttc ggtgccgggc cttgcctgac aaggaactgc gaaggctggt ccggacgggg
     4741 catctgaaat atcagtaggt gactttttta tcgaatccat gctagcctcc cggcggaggt
     4801 catccatgaa agccaaacgc agcgtgatcg agggcctggc ctcgatcctc ctcctcgccc
     4861 tcgtcttcgg cctcatgggc tcggcgatgg gccccgagaa cctgatcaac acgatcatga
     4921 agacggccta cgacctgctc atcaacaccg tcctctacct ggtcgcgatc accgtgatcg
     4981 tcggcgccct ctccgccttc ctcgtcgaat tcggcgtcgt caggatcgtc gagctcctcc
     5041 tcgcgccctt catgaagccg ctctaccgcc tgcccggcgt cgcctccctg ggcggcctcc
     5101 tctgcttcct ctcggacaat cccgccatcc tcaccctctc gcgggacaag ggctaccagt
     5161 cctacttcaa gaagtaccag ctctactcgc tcacgaattt cggcacctcc ttcgggatgg
     5221 gcctcatcgt cgtcacctac atgatcgggc agggccacct caacccggcg ggcgggggct
     5281 tctacgccga ggccctgatc ggcgtcgcgg gcgccgtctt cggctccgtc gtctccacgc
     5341 gcctcatgca ggcctccatc cggaagcgct tcggcgagga ggaggtcgtc cccgcctcgg
     5401 agaaggagtc cttcgacgag gacaaggtca tcgccaggaa gagggagagc gtcttcatcc
     5461 gcttcctgaa cgcgatgctg gacggcggca agtcgggcct ggacgccggc gtctccatca
     5521 tccccggcgt cctcgtgatc tccacggcgg tgatgatgct cacctggggg cccaaggacc
     5581 cggccgtcgg ctaccagggc ctggcctacg agggcgtgcc cctcctcccc gccctcgggc
     5641 gggccctcgg cttcctcttc acggccctct tcggcttcac gagccccgag gccgtcgcct
     5701 tccccgtgac ctccctgggc gccgtcggcg cggccctggg cctcgtgccc cgctatctca
     5761 aggaaggcct catcggcggc aacgacatcg ccgtcttcac cgcgatgggg atgtgctggt
     5821 cgggcttcct ctccacccat acggggatga tggacggact ggggcggcgc gacctcatcg
     5881 gcaaggccat cggctcccac gccgtcggag gcctggccgc cggcgtcttc gcgcactacc
     5941 tattcctggc cttccgggcc ctcggcgcgc tctagcctct tcgcgtatac tgggcgcaca
     6001 ggagaagcca tgagtctagc gcaagcgatc atcctcggcc tggtccaggg cctggccgag
     6061 ttccttcccg tgtcctcgag cggccacctt gtcctcttca aggacatcct gggcctctcc
     6121 gagctgcccc tcctctacga cgtcatcctc cacctcgcga ccctcctcgc gatcctcctc
     6181 gtcttcaggc ggcgcgtcct cgggatcctc tcctccctgg gccgctgggc ggcgaagcgg
     6241 aacggccggg aggacgccgc caacctggcc ctggtcgggc ccctcctcgt cgcgaccgtc
     6301 gtcaccgccg ccatcggctt cgcgatccag gaattcctcc ccgcggaggg cgtccgcctc
     6361 gtgtcgatcg agctcctcgt ctcggccgcc gtcctcgccg cctcggccct cctcaagccg
     6421 ggctccaagg gctacgcgga catcggggtc ggagaggggc tcttcctcgg cgtcgcccag
     6481 ggcctgggcg tcttctcggg catcagccgc tccggcatga ccatcaccgc aggcctggcc
     6541 gccggcatga ggcgggagga ggccggcgag ttctccttcc tcctcgccat ccccgccatc
     6601 ctcggggcct tcatcctcga gctcaaggac ctggggagcc tgcaggcctc ggtcgagccc
     6661 ctgcccctgg ccgtctccgc cctcgtcgcc ttcctctcgg gcctcctcgc cctctccctc
     6721 ctcatgcggg tcgtgaagcg gggcaagctg gcctggttcg cggcctatct cgtccccttg
     6781 ggcctcgcgg ggctgttcct cctctagaaa accgccgata gcttaaggta aggccgcccg
     6841 cgccccggcg ggaacgcccc ttccgggcgc cccgccggag cgacgggctc gtacgaggga
     6901 gccaacgcag catgaacgat tcgcctcgat ccaccgcggc cagcctggtc ctcggctcgc
     6961 tgctcgcgac agcggccctc tacctcctgg cgggcctcct cctctcggcc ggaggccggg
     7021 ccgggccggc cctcctcctc ctccccggcg agcgcctttt ctccgtcttc tcctgggcgg
     7081 ccttctacgc cccgctctgg ctcgccgccg ccgccgccat cgccttcgtc ccgggcttca
     7141 ggcccgccgc cctcttttcc ctcgcggcct ccctcgcgcc cttcctcctc ctcgcggcct
     7201 tcgcccgcct cgccgccgac ccggccgggg ccttcgcagc ctaccccctc ctcgaggccc
     7261 tgggccttcc cgccctgggc ttcgcggccc tcctcgccac gctcttcctc tgcctcctca
     7321 tcgcgcgcct cgccctcctc ttccccgccg gaggctccgg cgggagcgag agcggcgagg
     7381 gcgaggccgg cgacgatcgg cccggcggct atcccaggct cctgggcgcg ccggccggct
     7441 tcgggtcggc cgctgccgac ccctcggcct tggccgaggc aggcggcggc tccgagaccg
     7501 cgtctggccg cggcccgttc cgcctcggcc gcgcgggcca ggctggccgc gccggggccc
     7561 gcggcgcctc ccgcggcctc gcgggcgtcg acgcgcaggc cgggcaggac tcgatcctcg
     7621 agggccagga cggctacagc ttccgcgtgc ccgacttgcc cccgcccccc aggctgcgca
     7681 gcctgggcga ggcagcggat gaggacgacg atccgcctga ggccgccctc gagccgctcg
     7741 acccgctcga cccgct
//
LOCUS       DAOOSJ010000123         7736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5892_length_7736_cov_5.868377, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000123 DAOOSJ010000000
VERSION     DAOOSJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_7736_cov_5.868377"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLG14_07255"
     CDS             complement(<1..228)
                     /locus_tag="PLG14_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX48972.1"
                     /translation="MIVRNGELVRETRTRMRGGEGEVRVALLPQAGLAEHLRLAGEIV
                     IPPGSSIGAHAHEGETEWFLILEGRGEVDDDG"
     gene            complement(361..2307)
                     /locus_tag="PLG14_07260"
     CDS             complement(361..2307)
                     /locus_tag="PLG14_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX48973.1"
                     /translation="MTNASASPGPRKRSRGPGPSSLALLLAAVALLAGGGAAAAADKA
                     AAPGYIRVLCDENYPPYAFRDEEGRLEGIVVDQWKAWEKATGVSVEILGLPWAGALSA
                     FARGEAEVLDTVFATDERRENWLFTKPYAAVEVPVFIHKSLSGIAGPADLRGLKVAVK
                     DGDAAIGELVRRGVYELSLFPSYEAIIEAAARLDYRVFCVDGPPANYLLYKKRIDRDY
                     RVAFILNRGAFSRAVRKEEAGLMPLVERGFESLPKSAYEAIDRKWMGSELERRIDLRV
                     VALVGGAGAVAIAALFALAWILRRRVARATAELSEKLLLLEASEAKNRAFITALPDFF
                     FVFDKEGRFLEFSSSTPEFLAMSPAAFLGKRMAELGLPAGIAEGFEAKLAEALGTRRM
                     TTYEYELETGAGRRRFEGRIVPLGAERALLVARDVTEARSQEERINASLAEKEILLKE
                     IHHRVKNNMQVISSLIQLQSYAILDERDRELSRETQSRIRAMAAVHELLYRSPDLASI
                     EASDYLRSVSEELALGYDFGNIDFQGDATQLGIDEAVPLGLVANELILNAIKYAYGPG
                     RRGPVEASLRKEGAELVLRVRDEGRGLPPGLDPAASDSMGFTIVRSLAAQLGGSLAFG
                     GPPGFQAELRFVPPPRDRPGKGAF"
     gene            2530..3765
                     /locus_tag="PLG14_07265"
     CDS             2530..3765
                     /locus_tag="PLG14_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX48974.1"
                     /translation="MKRILAIGLALLAAAALFVSCGAKGSNELVIQSNASDQAPKDQL
                     QILADQFMKENPGIKVTINTTAHEQFKTQLMNYLTAKDAPDVLTWFAGYRMQQYAAKG
                     LLEPLDSIWQDSEFPAAFKTASSYNGKTYFMPSSWYWWAVYYNKDVFAKLNLSVPKTW
                     EEFEAVCAALKKAGIEPIAIGAKDTWTAGGWFDYMNVAVNGGALHQDLTAGKVSYTDP
                     KVANTFNYIADLSKKGYFMKNAASYSWQEAATVMFDGKAGMYLMGQFIKDVAPADKKD
                     KIDWFQFPIVGNNTAYGVDTPIDGYFVPAKAKNKANALKFAAFLQRKDVQESYCKALG
                     RLAANQNVAPPNADAQRGLDMVKGAATAMQFYDRDAPEEMAAKGMNAIVDIIGTPDKA
                     GDILAALDKERERIYAELK"
     gene            3885..4772
                     /locus_tag="PLG14_07270"
     CDS             3885..4772
                     /locus_tag="PLG14_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOX48975.1"
                     /translation="MPSRRIRKGALAPWAFLALPLAMYLVWVVVPIFESFAFAFTKRD
                     SIVFGTVWNGFANFRRLAGDGQFWLAIKNNLLWLVFFVGVPVPLGLLVAMLFNGGRRG
                     SRAYKTLFYLPMTLSFTIIGTIWMWIYNPDFGALNTFLRSIGLDGLAVQWLGDKRYMT
                     GALIAVGVWRQVPYVMILYLAGLQGVPPELIEASLIDGANWRQRFRNVILPLLMPATV
                     VAVTISVIDSLRSFDIVYVMTNTKARAAEVLASYMYSSAFQYADFGYGSAIAVVQFLI
                     TLAFILVYVTDVLRKESER"
     gene            4793..5644
                     /locus_tag="PLG14_07275"
     CDS             4793..5644
                     /locus_tag="PLG14_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOX48976.1"
                     /translation="MKTVTSPAARTLRAVAFYAAATFLVVLWLLPLVIGAFTSLKSMD
                     ELMSDPVMWRAPKVLHFENFAEAWERAGMGRYIVNTFIVTIPSVVGALAVSSLAAFAL
                     AFYRFRLNKAVLILFVAGMLIPFQMLMIPVFRMSNAIGIDDSYLGVILFHVSFQLGFC
                     TFFLRNFMKQIPYSQIEAPRIDGAGDFTIYSKIILPLALPSIAALAVLEFTWIWNDLL
                     WSLVLLRSDALKTVTLGLANMQGEFISTYNLTAAGSILAAIAPLAVFLAFQRYFIEGL
                     TMGADKG"
     gene            5650..6624
                     /locus_tag="PLG14_07280"
     CDS             5650..6624
                     /locus_tag="PLG14_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX48977.1"
                     /translation="MGALAAAGLAVGALLLLGAAAGFGLAAVVVFPRRFALPETEAIE
                     REKGFLAAAGWDGLSPREVCVAAPDGRSLAAAYVPLEGSRRTVVLVHGITYTRWGSVK
                     YLRAFRSLGFNALLVDQRGHGASGRGPVTFGFREAPDLRAWVDWALAELGPGGLVGLH
                     GESMGAAVAIQEAAIDPRVAFVVADCPFSDLRSLLAHRLRVEFGLPAFPFLGLAESAA
                     RLLAGGFRFREVSPARAAAAFRAPLLLVHGLSDDYIPSSMAREIAAAREAAGLPFRLV
                     LVPGAAHAKSWETCPEAYERAVAEFFGGLRAPGPAEPSSLPAATAPGG"
     gene            6705..>7736
                     /locus_tag="PLG14_07285"
     CDS             6705..>7736
                     /locus_tag="PLG14_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOX48978.1"
                     /translation="MPTRAARGRETTAPSRREDDRLYEKLSLIIDSLDDLYKLDKRDF
                     LRKVFEDAFRLIPEAEKGSLYEFDGERYRPVFGRGYDFATLSRLSFSPQEAFIDFEVD
                     AEGEIRAYETLIEGRDSERFDPETIAVFRALGTYEGFVSLYAPIRVDGAVAGLISLER
                     FERSGYPRLSRKVLEYYARLISEFYSQKLYQEKETRLYRDVVSSLVSAIEVMDRYTEG
                     HAKRVAEYSRLIGVKLGLSGRDLEDVETAALLHDVGKLGVPTEILRKPARLEPEEYEL
                     VKRHPANAAKILGEIRGFERIVEYAYRHHERYDGSGYPGGLSGDAIPLGAQVIQVADA
                     IDAMTSERSY"
BASE COUNT         1185 a         2822 c         2485 g         1244 t
ORIGIN      
        1 cccgtcgtcg tcgacctcgc ccctgccttc gaggatgagg aaccactcgg tctccccttc
       61 gtgggcgtgg gcgccgatgc tcgacccggg cgggatcacg atctcgcccg cgagccgtag
      121 gtgctcggcc aggcccgcct gggggaggag ggcgacgcgg acctcgccct cgccgccgcg
      181 catcctggtc cgcgtctcgc ggaccagctc gccgttccgt acgatcatgt cgcctcccct
      241 cgggccgagg gcccgcctgg aacccatgat agcacggccg ccggcggcgc ccaaccgctg
      301 ccggcgtccc cgcaacggcc aggcgggggc gctggccgga cccgacaggg aaggccgagc
      361 ctagaaggcg cccttcccgg gccggtcccg gggcgggggg acgaagcgca actcggcctg
      421 gaagcccggc ggacccccga aggcgaggct gcccccgagc tgggcggcga ggctccgcac
      481 gatggtgaag cccatagagt cgctcgcggc cggatcgagg ccgggcggca ggccccggcc
      541 ctcgtccctg accctgagca cgagctccgc gccctccttg cggaggctcg cctccacggg
      601 gccgcgccgg cccgggccgt aggcgtactt gatcgcgttg aggatgagct cgttcgcgac
      661 gaggccgagg ggcacggcct cgtcgatgcc gagctgggtc gcgtcgccct ggaaatcgat
      721 gttcccgaag tcgtagccga gcgcgagctc ctcggacacc gagcgcaggt agtctgaggc
      781 ctcgatggag gcgaggtcgg gcgagcggta gaggagctcg tggacggcgg ccatggcgcg
      841 gatgcgggac tgggtctcgc gggagagctc gcggtccctc tcgtccagga tcgcgtagga
      901 ctggagctgg atcaggctcg agatcacctg catgttgttc ttgacgcggt ggtggatctc
      961 cttgaggagg atctccttct ccgccagcga ggcgttgatc ctctcctcct ggctcctcgc
     1021 ctccgtgacg tcccttgcga ccaggagggc gcgctcggcc ccgaggggga cgatccgccc
     1081 ctcgaacctc cgcctgccgg cgccggtctc gagctcgtac tcgtaggtcg tcatgcgccg
     1141 ggtcccgagc gcctccgcga gcttcgcctc gaagccctcg gcgatgccgg cgggcaggcc
     1201 gagctcggcc atcctcttcc ccaagaaggc cgccggggac atggccagga actccggcgt
     1261 ggagctcgag aactcgagga agcgaccctc cttgtcgaag acgaagaaga agtcgggcag
     1321 ggcggtgatg aaggcgcggt tcttcgcctc gctggcctcg aggaggagga gcttctccga
     1381 gagctccgcc gtggcccggg cgacgcgccg cctgaggatc caggccaggg cgaagagcgc
     1441 ggcgatggcg acggctcccg cgcctccgac cagcgcgacg acgcgcaggt cgatccggcg
     1501 ttccagctcg gagcccatcc acttccggtc gatggcctcg taggcggact tgggcaggga
     1561 ctcgaagccc cgctccacga ggggcatgag gccggcctcc tccttccgca cggcgcggct
     1621 gaaggcgccc cggttcagga tgaaggccac gcggtagtcc cggtcgatcc gcttcttgta
     1681 gaggaggtag ttggccggcg ggccgtcgac gcagaagacg cggtagtcga gtcgggcggc
     1741 cgcctcgatg atggcctcgt agctcgggaa gagggagagc tcgtagacgc cgcgccgcac
     1801 cagctcgcct atggccgcgt cgccgtcctt caccgcgacc ttgaggccgc gcaggtccgc
     1861 ggggccggcg atccccgaga gggacttgtg gatgaagacg gggacctcca cggccgcgta
     1921 gggcttggtg aagagccagt tctcccggcg ctcgtccgtc gcgaagacgg tgtcgaggac
     1981 ctcggcctcg ccacgggcga aggcggagag ggccccggcc cagggcaggc ccaggatctc
     2041 cacggagacg ccggtcgcct tttcccaggc cttccattgg tcgacgacga tgccctcgag
     2101 cctgccctcc tcgtcccgga aggcgtaggg agggtagttc tcgtcgcaga ggacgcggat
     2161 gtagccgggc gcggcagcct tgtccgccgc cgccgcggcg ccgccgccgg caaggagggc
     2221 cacggccgcg aggaggaggg cgagggagga aggccccggt ccccggcttc ttttccgggg
     2281 cccaggcgac gcgctggcgt tggtcaccgt cttactataa cgcgtgcctg tatgcgggca
     2341 aagcgcgaac gcgcattcct cggagttttc ttaaggtttc gcccgggccg cgaggcgcgg
     2401 cgccgtctcg attcccggtc ccggtccggc gatggcggag acagcggaaa gggtgttgac
     2461 agccgtcgat agtggagtaa aatgaaagcg cttacattaa gtttgtttct gaaagtaagg
     2521 aggaaccaca tgaagcgaat cttggccatc ggcctcgctc ttctcgccgc ggcggcgctc
     2581 ttcgtctcct gcggagcgaa gggctccaac gagctcgtca tccagtccaa cgcctccgac
     2641 caggccccga aggaccagct ccagatcctg gccgaccagt tcatgaagga gaatcccggg
     2701 atcaaggtga cgatcaacac cacggcgcac gagcagttca agacccagct catgaactac
     2761 ctcaccgcca aggacgcgcc ggacgtcctc acctggttcg ccggctaccg catgcagcag
     2821 tacgcggcca agggcctcct cgagcccctc gactcgatct ggcaggacag cgagttcccc
     2881 gccgccttca agaccgcctc cagctacaac gggaagacct acttcatgcc ctcctcctgg
     2941 tactggtggg ccgtctacta caacaaggac gtcttcgcca agctgaacct ctccgtgccc
     3001 aagacctggg aggagttcga ggccgtctgc gccgccctca agaaggccgg catcgagccc
     3061 atcgccatcg gcgccaagga cacctggacc gccggcggct ggttcgacta catgaacgtc
     3121 gccgtcaacg gcggggctct ccaccaggac ctgaccgcgg gcaaggtctc ctacaccgac
     3181 cccaaggtcg cgaacacctt caactacatc gccgacctct ccaagaaggg ctacttcatg
     3241 aagaacgcgg cgagctactc ctggcaggag gccgccaccg tgatgttcga cggcaaggcc
     3301 ggcatgtacc tcatgggcca gttcatcaag gacgtcgccc ccgccgacaa gaaggacaag
     3361 atcgactggt tccagttccc gatcgtcggc aacaacacgg cctacggcgt cgacaccccc
     3421 atcgacggct acttcgtccc cgccaaggcc aagaacaagg ccaacgccct caagttcgca
     3481 gccttcctcc agaggaagga cgtccaggag agctactgca aggccctcgg ccgcctggcc
     3541 gcgaaccaga acgtcgcccc ccccaacgcc gacgcccagc gcggcctcga catggtgaag
     3601 ggagccgcga ccgcgatgca gttctacgac cgcgacgctc cggaggagat ggcggccaag
     3661 ggcatgaacg cgatcgtcga catcatcggc acccccgaca aggccggcga catcctcgcc
     3721 gccctggaca aggagcgcga gcgcatctac gccgagctca agtaaggctc gacccgatcg
     3781 accccgggcc cgccggccag atccctccgg ccggcgggcc cttttttccg gagggtagcg
     3841 cctccccgcg ccttgcggcg gaccggcgaa aggagacgaa agccatgccc tcgaggcgga
     3901 tcaggaaagg ggccctcgcg ccctgggcct tcctcgcgct tcccctcgcg atgtacctcg
     3961 tctgggtcgt cgtgcccatc ttcgagagct tcgccttcgc cttcaccaag agagactcga
     4021 tcgtgttcgg cacggtctgg aacggcttcg cgaacttcag gcggcttgct ggcgacggcc
     4081 agttctggct cgcgatcaag aacaacctgc tctggctcgt cttcttcgtg ggcgtgcccg
     4141 tgcccctggg gctcctggtc gcgatgctct tcaacggcgg gcgccgtggc agccgcgcct
     4201 acaagaccct cttctacctg ccgatgaccc tctccttcac gatcatcggg accatctgga
     4261 tgtggatcta caacccggac ttcggcgccc tcaacacctt cctccgctct atcgggctcg
     4321 acggcctggc cgtgcagtgg ctcggcgaca agcgctacat gacgggggcc ctgatcgcgg
     4381 tcggcgtgtg gcgccaggtg ccctacgtga tgatcctcta cctcgcgggc ctccagggcg
     4441 tgccgcccga gctcatcgag gcctccctca tcgacggggc gaactggcgc cagcgcttcc
     4501 gcaacgtcat cctccccctc ctcatgcccg cgacggtcgt ggccgtgacg atctccgtga
     4561 tcgactccct gcgctccttc gacatcgtct acgtgatgac gaacaccaag gcccgcgcgg
     4621 ccgaggtcct ggcgagctac atgtactcca gcgccttcca gtacgccgac ttcggctacg
     4681 gctcggccat cgccgtcgtc cagttcctca tcacgctggc cttcatcctg gtgtacgtca
     4741 ccgacgtgct caggaaggaa tccgagcgct aggcccaagg agggagcaat cgatgaagac
     4801 cgtaaccagc cccgccgcga ggaccctgcg cgccgtcgcc ttctacgccg cggccacctt
     4861 cctcgtcgtc ctgtggctcc tgcccctcgt catcggggcc ttcacctcgc tcaagtcgat
     4921 ggacgagctc atgtccgatc ccgtgatgtg gcgggccccc aaggtcctcc atttcgagaa
     4981 cttcgcggag gcctgggagc gggcgggcat gggccgctac atcgtcaaca ccttcatcgt
     5041 gaccatcccc tccgtcgtcg gagccctggc cgtctccagc ctcgccgcct tcgccctggc
     5101 cttctaccgc ttccggctca acaaggccgt cctcatcctc ttcgtggcgg gcatgctcat
     5161 ccccttccag atgctcatga tccccgtctt ccggatgtcg aacgcgatcg gcatcgacga
     5221 cagctacctg ggcgtcatcc tcttccacgt ctccttccag ctcggcttct gcaccttctt
     5281 cctcaggaac ttcatgaagc agatccccta ctcgcagatc gaggccccga ggatcgacgg
     5341 cgcgggcgac ttcacgatct actcgaagat catcctgccc ctggccctgc cgagcatcgc
     5401 ggccctggcc gtcctcgagt tcacctggat ctggaacgac ctcctctggt ccctcgtcct
     5461 cctgcgctcc gacgcgctca agaccgtgac cctgggcctc gcgaacatgc agggcgagtt
     5521 catctccacc tacaacctca cggccgccgg ctcgatcctg gcggcgatag cgcccctcgc
     5581 cgtcttcctg gccttccagc gctacttcat cgaggggctc acgatgggcg ccgacaaggg
     5641 ctaggcgcga tgggcgcgct cgccgccgcg gggctcgcgg tcggcgccct ccttctcctc
     5701 ggcgcggccg cgggcttcgg cctcgcggcc gtcgtcgtct tcccccggcg cttcgcgctt
     5761 ccggagaccg aggcgatcga gagggagaag ggcttcctgg ccgcggccgg ctgggacggc
     5821 ctttcccccc gcgaggtctg cgtcgccgcg cccgacggcc gcagcctcgc cgcggcctac
     5881 gtgcccctgg aaggctcgcg gaggaccgtc gtcctggtcc acggcatcac ctacacgcgc
     5941 tggggctcgg tgaagtacct ccgcgccttc aggtcgctgg gcttcaacgc ccttctcgtc
     6001 gaccagcgcg gccacggcgc ctcgggccgc ggccccgtga ccttcggctt ccgcgaggcc
     6061 cccgacctcc gcgcctgggt ggactgggcc ctggccgagc tcgggcccgg cggccttgtc
     6121 gggctgcacg gagagtccat gggcgcggcc gtcgccatcc aggaggcggc gatcgacccg
     6181 cgcgtcgcct tcgtcgtcgc ggattgcccc ttctccgacc tccgctccct cctcgcccac
     6241 aggctccgcg tcgagttcgg cctgcccgcc tttcccttcc tcggcctggc ggagtcggcg
     6301 gcgcggctcc tggcgggggg cttccgcttc cgcgaggtct cgcccgcccg cgccgccgcg
     6361 gctttccgcg cgcccctcct cctcgtccac ggcctttcag acgactacat cccctcctcg
     6421 atggcgcgcg agatcgccgc agcgcgcgaa gccgcgggcc tgcccttcag gctcgtcctc
     6481 gtcccgggcg cggcccacgc caagtcctgg gagacctgcc ctgaggccta cgagagggcg
     6541 gtcgcggaat tcttcggcgg gcttcgcgcc cccggcccgg ccgagccgag ctctcttccc
     6601 gccgctacgg cgccgggcgg ttgatcaaag ccggacggcg ctgtaatcta tcccgcccag
     6661 ggccttgcgg cgaaggcccg cgctcgcgac gacggaggaa acgcatgccg acacgggccg
     6721 ccagggggcg tgaaaccaca gctcccagcc gacgggaaga cgaccgcctc tacgagaagc
     6781 tctccctcat catcgattcc ctggacgacc tctacaagct cgacaagcgg gacttcctga
     6841 ggaaggtctt cgaggacgcc ttccggctca tccccgaggc tgagaagggc tccctctacg
     6901 agttcgacgg ggagcgctac cgccccgtct tcgggcgcgg ctacgacttc gcgacgctct
     6961 cgcggctctc cttcagcccc caggaggcct tcatcgactt cgaggtggac gccgaggggg
     7021 agatccgcgc ctacgagacc ctcatagagg gccgggactc cgagcgcttc gatcccgaga
     7081 cgatcgccgt gttccgggcc ttgggcacct acgagggctt cgtctcgctc tacgcgccga
     7141 tccgcgtgga cggcgcggtg gccgggctca tcagcctgga gcgcttcgag cgctccggct
     7201 acccgcgcct ctcgcgcaag gtcctcgagt actacgcccg cctgatctcg gagttctact
     7261 cgcagaagct ctaccaggag aaggagacgc ggctctaccg cgacgtcgtc tcctccctcg
     7321 tctcggcgat cgaggtgatg gaccgctata ccgaaggcca cgcgaagcgc gtcgccgagt
     7381 acagccgcct catcggcgtc aagctcggcc tttcgggccg cgacctggag gacgtggaga
     7441 cggcggccct cctccacgac gtgggcaagc tcggcgtccc gacggagatc ctcaggaagc
     7501 ccgcccgcct cgagcccgag gagtacgagc tcgtgaagcg gcatcccgcg aacgcggcca
     7561 agatcctggg cgagatccgg ggcttcgagc gcatcgtcga gtacgcctac cgccaccacg
     7621 agcgctacga cggaagcggc tacccgggcg gcctatcggg cgacgcgatt ccgctcggcg
     7681 cgcaggtgat ccaggtggcc gacgccatcg acgccatgac gagcgagcga tcctac
//
LOCUS       DAOOSJ010000124         7732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5899_length_7732_cov_5.854110, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000124 DAOOSJ010000000
VERSION     DAOOSJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_7732_cov_5.854110"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /gene="priA"
                     /locus_tag="PLG14_07290"
     CDS             <1..1947
                     /gene="priA"
                     /locus_tag="PLG14_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOX48979.1"
                     /translation="YLKVAFNVPLPEAYTYRNDPASPASVGLRVYAPLGRREALGYVV
                     GELDEPDLDEAKIKRITRTVDKEPLFDGRSVELARWLSGMYYCGLGEALGAMLPSGKR
                     EASVPAIEAEDFEIGEKPLALSSEQAAALSRITRERSGIHYLYGLTGTGKTEVFLQAA
                     EATLAEGRGVIYLVPEIALTGQVVEQAERRFGAKCSVIHSRVTPSRKLAEWRRILRGE
                     AQVVIGARSAVFAPLPNLGLVVIDEEHESTYKSGSAPRYHARQVAMKRRAMEGARLVM
                     GSATPSAEAWSLMEEGQVERLPLTRRLSGGAVPSVEVVDMRGEEGAISHRLAEAVRET
                     ARAGGQSILFLNRRGFSYFFRCRSCGAEIRCRHCSVALTYHKDRNALVCHYCGYRAAP
                     PRACPDCGSLDVGWAGFGTERVEEEAARLFPDLRLRRLDADSTSRKGELEAALADFRE
                     GRADLLLGTQMVAKGLNFPRVKTVGVVLADTTLNLPDFRAAERAFSLVVQVAGRAGRF
                     SPDGRVFVQTFRPESPVIRLAAALDAPSFYRLELEARRELGFPPYSRLVRIVFRSKEK
                     EKAKEAARSFAEAATRVLPPEAEILGPAECPLGMVAGTARWQVLFRAPELRAIHAPLG
                     ALLASWEVPSGLRLEADVDPASLM"
     gene            2002..3180
                     /locus_tag="PLG14_07295"
     CDS             2002..3180
                     /locus_tag="PLG14_07295"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOX48980.1"
                     /translation="MRATVYWYSGTGNSWLAAGRLAERLREGGAEAVLVPIEDAARVA
                     PAARADARAAGAGGAPDGGEAVHGGAPDLEVFCFPIYAFDAPSAVARFVAGLPLPSAA
                     LADTGASASAPAGAQASAPRKAALVATMGGKGYEGRALRRLERILASRGRKVVLAEAL
                     EMPEAFVQAYPATPPEAAEARTAAALAIADGLAGELLSGGGRFRADRPALRILLTPVS
                     VLFALVARRLLGLSWGCDGLACTGCGLCARACPAGNIRMAGQRPSFGAACEDCQRCAN
                     LCPTGAIRVSALKLLVYVILSFPSYGRILAGLLGLPPPAGPLGLLLRAALWLVGAFAA
                     FAAAASAFRVAESRPAGRNFLSRSYAKGFLRRLAPGFQAELARRDRAAREKAGATDRT
                     "
     gene            3274..3639
                     /locus_tag="PLG14_07300"
     CDS             3274..3639
                     /locus_tag="PLG14_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOX48981.1"
                     /translation="MDIEFLTVRTKDLAASVAFWERVLGFKVERRFSPRPGMEIAFLV
                     DGSGGRVEFIQEEGKPAFSGSGVSVGFRVPDAAAAAASLAAQGVELVYGPIAMQSGVK
                     LLGARDPNGLELGFVEEAR"
     gene            complement(3715..6516)
                     /locus_tag="PLG14_07305"
     CDS             complement(3715..6516)
                     /locus_tag="PLG14_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48982.1"
                     /translation="MRRTPRLAPLLVLALAALLPPRPAGAGPLVAPGQRLYVVKTEHF
                     DIIFPKASEPSALRLASFAESVYDEVAGKLESGLPRRVPVLVTPDIATFNGFMNPLPY
                     FHIVLFDTPLPMGWTAFRDNFRALFLHELAHAVSLQVTTPFAAFLSGVFGSYMKPALF
                     TAPLFMVEGVTVSFESAPGEGGRAADPLVRQRLRQDILENRFKSPTEATGVWDDYPGG
                     SVYYEYGGLFSAYLQERYGMEAYAGLWRAMVFGAPSLSLDPDRRGFAKAFRKAYGIGL
                     AEAWADFRLSLTILGVRDVPEWLAPPGGASIRGLASGGGGLFWVDGEASRAWRLDAAT
                     GRKEALFDADGRTAITDASPDGARLAVQRSLGLADGRDRIESLVYDTRTRRFVEGSAA
                     PDLREARFYREGLVGVRSRLHVGDLVYASPEGERVLLRGGETLALSSPAPLPDGRIAL
                     VLAVEGQRRIGILEPESGRLVLVRPEAGEEGALGFVRQVSAAGDRLWFSYNDDDRMYK
                     LGRLEGGRVHLESTDYSGGVLLPVEEGGRVYYVGRFSEGESPCRYPGEASGLGDRVLG
                     WTEEEYAPAEPLPAAPDAQAPEILPYRPLAYANPFNAWLLYPDLDTLDRSFRVLASFG
                     FADPAENNHATLKAGYDAGYGFAEAGLEWGNRSLPVGFDLALGDSLVYSSADYPERQS
                     SASLAAHVGLPLYPGTRSLELGLGGSFLLRAAGGEGSPYSWAYAEPEYVGSAWLGLYG
                     RVPGRAWESARGLDVTSWHDLAVESSVYKTEAQVLLSADALPLRLELWGAWATGRILG
                     LDASSAVFAADRRPAYVEYQDLAADRESLLAQGDLSLRLAAQRIGADVLGLYLRRLVV
                     DAGYRGAYVSGGYLQSCYGRLSLDLGLGFGATGGLPLRAYAEGFARLSDLAAGERLGW
                     RLGIGLGGDGSIRTGAARR"
     gene            complement(6614..7243)
                     /locus_tag="PLG14_07310"
     CDS             complement(6614..7243)
                     /locus_tag="PLG14_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48983.1"
                     /translation="MGLGPGTRAVLFDIDGVLLHHGAHFYETLALAGRESPREAIGRF
                     YSSGTNLRCDKGELDPELALAPFLAEMGFPGSAADYLAAQYAFEERFVDLALLEGVRE
                     LRASGLSCYVASNQNPRRKARLVEWLSVEESFDGAFFSCDLGHVKAEDGYWEAVLESL
                     AAREGLAARDLLFLDDLSDNLRKAEEHGLATRLIEGKEGILAFLAEARS"
     gene            complement(7234..>7732)
                     /locus_tag="PLG14_07315"
     CDS             complement(7234..>7732)
                     /locus_tag="PLG14_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48984.1"
                     /translation="DAPALFAYRSLPEVQRFQSFRPAGLAEAEAFIRAYPAAFAPGGG
                     WSQLGVFLEGGLIGDLGARLYGTEGLEAEIGYTIAPARQGKGYGKEAVARLLDFLFLE
                     LRARRAVASLDPKNLASAALLSGLGFRREGLFLESVLVDGVWADDLRFAILAREWEER
                     RGAWA"
BASE COUNT         1033 a         2810 c         2871 g         1018 t
ORIGIN      
        1 tacctcaagg tcgccttcaa cgtccccctc ccggaggcct acacctaccg gaacgacccc
       61 gcctcgccgg cctccgtggg cctgcgcgtc tacgcgccct taggccgccg cgaggccctg
      121 ggctacgtcg tgggcgagct cgacgagccg gacctggacg aggccaagat caagcggata
      181 acgcggacgg tggacaagga gcccctcttc gacgggcgga gcgtcgagct cgcgcgctgg
      241 ctctccggca tgtactactg cggcctgggc gaggccctgg gcgcgatgct cccctcgggg
      301 aagcgcgagg cctccgttcc cgcgatcgag gcggaggact tcgagatcgg ggagaagccc
      361 ctcgccctct cctcggagca ggccgccgcc ctctcgcgca taacccgcga gcgctcgggc
      421 atccactacc tctacggcct cacgggcacg gggaagacgg aggtcttcct ccaggccgcg
      481 gaggcgaccc tggcggaggg ccgcggggtc atctacctcg tccccgagat cgccctcacg
      541 ggccaggtgg tcgagcaggc ggagcgccgc ttcggcgcga agtgctcggt gatccactcc
      601 cgcgtgacgc cttcccgcaa gctcgccgag tggcggcgca tcctccgcgg cgaggcccag
      661 gtcgtgatcg gcgcgcggag cgcggtcttc gcgcccttgc cgaacctggg gctcgtcgtc
      721 atcgacgagg agcacgagtc cacctacaag tccggcagcg cgccgcgcta ccacgcgcgg
      781 caggtcgcga tgaagcgccg ggccatggag ggcgcccgcc tcgtgatggg gagcgcgacg
      841 ccctccgccg aagcctggag cctcatggag gagggccagg tcgagcgcct tcccctcacg
      901 cgccggctct cgggcggcgc cgtcccctcc gtggaggtcg tcgacatgcg cggcgaggag
      961 ggggccatct cccatcgcct cgcggaggcg gtgcgcgaga ccgcgagggc cggcggccag
     1021 tccatcctct tcctgaaccg ccgcggcttc tcctacttct tccgctgcag gagctgcggc
     1081 gcggagatcc gctgccggca ctgctccgtc gccctgacct accacaagga ccggaacgcc
     1141 ctggtctgcc actactgcgg ctatcgcgcc gccccgcccc gggcctgccc cgactgcggc
     1201 tccctggacg tgggctgggc gggcttcggc acggagcggg tggaggagga ggcggcgcgg
     1261 ctcttcccgg acctgcgcct aaggcgcctg gacgccgact ccacctcccg caagggcgag
     1321 ctcgaggcgg ccctggccga cttccgtgag ggcagggcgg acctgctcct cgggacgcag
     1381 atggtcgcga agggcctcaa cttcccgcgc gtgaagaccg tcggcgtcgt cctcgcggac
     1441 acgaccctga acctccccga cttccgcgcc gcggagcgcg ccttctccct ggtcgtccag
     1501 gtcgcgggcc gggcgggacg cttctccccc gacggccgcg tcttcgtgca gaccttccgg
     1561 ccggagagcc ccgtgatccg cctggccgcg gccctggacg cgccctcctt ctaccgcctg
     1621 gagctggagg cgcggcgcga gctcggcttc ccgccctact cgcgcctcgt gcggatcgtc
     1681 ttccgctcca aggagaagga gaaggcgaag gaggccgcgc ggtccttcgc ggaggcggcc
     1741 acgcgcgtcc tcccgccgga ggcggagatc ctgggccccg ccgaatgccc cctcgggatg
     1801 gtggcgggca ccgcgcgctg gcaggtcctg ttccgcgccc ccgagctccg cgccatccac
     1861 gcccccctgg gcgccctcct cgcctcctgg gaggtcccct ccggcctgcg cctcgaggcc
     1921 gacgtcgatc ccgccagcct catgtaggac agcccgggcg cgccgcggcg cggcccgggc
     1981 cggactttga aaggaagcga tatgcgagcg accgtctact ggtactccgg aacaggcaac
     2041 agctggctgg ccgcgggccg cctcgcggag cgcctgcgcg agggcggcgc ggaggccgtc
     2101 cttgtaccta ttgaggacgc cgcgcgcgtc gccccggccg ctcgcgccga tgcccgcgcc
     2161 gcgggagcag gaggcgcgcc ggacggaggc gaggccgtcc acggcggcgc gccggacctc
     2221 gaggtcttct gcttcccgat ctacgccttc gacgcgccct ccgccgtcgc gcgcttcgtc
     2281 gccggcctgc cgctcccgtc cgcggccttg gccgacaccg gcgcttccgc aagcgctccg
     2341 gccggcgccc aggcctccgc gccgcgcaaa gcggccctag tcgcgacgat gggcgggaag
     2401 ggctacgagg gccgggcctt gagacgcctt gagcggatcc tcgcgtcgcg ggggcgcaag
     2461 gtcgtcctgg cggaggccct ggagatgccg gaggccttcg tccaggccta tcccgcgacg
     2521 ccgcccgagg ccgcggaggc gcgcacggcc gcggccctcg ccatcgccga cgggctcgcc
     2581 ggggagctgc tctcgggcgg cggccgcttc cgcgccgaca ggcccgcctt gcggatcctc
     2641 ctcacgcccg tctccgtcct cttcgccctc gtcgcgcggc gcctcctcgg cctctcctgg
     2701 ggctgcgacg gccttgcctg cacgggctgc ggcctctgcg cccgcgcctg ccccgcgggc
     2761 aacatccgca tggcggggca acggccctcc ttcggcgcgg cctgcgagga ctgccagcgc
     2821 tgcgcgaacc tctgcccgac gggcgcgatc cgcgtctccg ccctgaagct cctcgtctac
     2881 gtcatcctgt cgttcccgtc ctacgggagg atcctcgcgg gcctcctcgg cctcccgccg
     2941 cccgcggggc ccctcggcct cctcctccgc gcggcgctct ggctcgtggg cgccttcgcg
     3001 gccttcgccg ccgcggcctc tgccttccgc gtcgcggagt cgcggcccgc cgggcgcaac
     3061 ttcctctccc gctcctacgc gaagggtttc ttgcggcggc tcgctccggg cttccaggcg
     3121 gagctcgcga ggcgggacag ggccgcgcgg gagaaggcgg gggcgacgga ccggacatga
     3181 cagcgcggcg gccccgtcgc cgccgatcca tcggcagttg atcctcgagg gcggccgtca
     3241 gcgccgccca tcgcgcgcga aaaggagcgt cacatggaca tcgaattcct caccgtccgc
     3301 acgaaggact tggccgcctc ggtggccttc tgggagaggg tcctgggctt caaggtcgag
     3361 cgccgcttct cgccgcggcc gggcatggag atcgccttcc tggtcgacgg ctccggcggc
     3421 cgggtcgagt tcatccagga ggaggggaag cccgccttct cgggctcggg cgtctccgtg
     3481 ggcttccgcg tgccggacgc cgcggcggcc gcggcctccc tcgccgccca gggcgtcgag
     3541 ctcgtctacg ggccgatcgc gatgcaaagc ggcgtgaagc tcctcggggc gcgggacccg
     3601 aacggcctgg agctcggctt cgtggaggag gcccgctagg cgggcttcag cgcttcgcgg
     3661 ccttcggcgc atcgcggccg gtggcgccgc gggggctatc gcgcctcgcg gccttcagcg
     3721 ccgcgcggcc ccggtccgga tggagccgtc gccgcccaag ccgatgccca ggcgccagcc
     3781 caggcgctcg cccgcggcca ggtccgagag ccgggcgaag ccttcggcgt aggcgcgcag
     3841 gggcaggccg cccgtggcgc cgaagcccag gcctaggtcg agggagagcc ttccgtagca
     3901 ggactggagg tagccgccgc ttacgtaggc gccgcggtag cccgcgtcga ctacgaggcg
     3961 cctcaggtag aggccgagga catcggcgcc gatccgctgc gcggcgagcc gcagggagag
     4021 gtcgccctgc gcgaggaggc tttccctatc cgccgcaaga tcctggtact cgacgtaggc
     4081 ggggcggcgg tcggccgcga agacggcgct cgaggcgtcg aggccgagga tgcggccggt
     4141 cgcccaggcg ccccagagct cgagcctgag ggggagggcg tcggcggaga ggaggacctg
     4201 ggcctccgtc ttgtagaccg agctctccac ggccaggtca tgccagctcg tgacgtccag
     4261 gccccgcgcg ctctcccagg ctcggccggg cacccggccg tagaggccga gccaggccga
     4321 gccgacgtac tcgggctccg cgtaggccca ggagtaggga ctgccttctc cgccagcggc
     4381 ccggagcagg aaggagcctc ccaggccgag ctccaggctc ctcgtccccg gatagagcgg
     4441 caggccgaca tgggcggcga gggaggccga gctctggcgc tcagggtagt cggcgctcga
     4501 gtagacgagg ctgtcgccca gggcgaggtc gaagcccacg gggaggctcc ggttccccca
     4561 ttccagcccc gcctcggcga agccgtagcc cgcgtcatag ccggccttga gcgtggcgtg
     4621 gttgttctcc gccggatcgg cgaagccgaa ggaggcgagg acgcggaagg agcggtccag
     4681 cgtgtccagg tcgggataga ggagccaggc gttgaaggga ttggcgtagg cgaggggccg
     4741 gtagggaagg atctccggcg cctgggcgtc cggcgcggcg gggagcggct ccgcgggggc
     4801 gtactcctcc tccgtccagc caaggacgcg gtcgcccagg cctgaggcct cgccggggta
     4861 gcggcagggc gactcgccct cggagaagcg gccgacgtag tagacccggc ccccctcctc
     4921 cacgggcagg aggacgccgc ccgagtagtc cgtcgactcg agatggacgc gcccgccctc
     4981 cagccggccg agcttgtaca tccggtcgtc gtcgttgtag ctgaaccaga gcctgtcgcc
     5041 cgcggccgag acctgccgca cgaagccgag ggctccctcc tctccggcct cgggccggac
     5101 caggacgagc ctccccgact cgggctccag gatgccgatg cggcgctgtc cctccacggc
     5161 caggaccagg gcgatccggc cgtcgggaag aggcgcgggc gaggagagcg cgagcgtctc
     5221 gccgccccgc aggaggaccc gctccccctc gggcgaggcg tagacgaggt cgccgacgtg
     5281 cagccgggac cggacgccga ccaggccttc gcggtagaag cgcgcctccc ggaggtcggg
     5341 cgccgcgctc ccctcgacga agcggcgggt ccgcgtgtcg tagaccagac tctcgatccg
     5401 gtcccggccg tcggccagtc ccaggctgcg ctggaccgcg aggcgcgctc cgtcgggcga
     5461 ggcgtcggtg atcgcggtcc ggccgtcggc gtcgaagagg gcctccttcc tccccgtcgc
     5521 cgcgtcgagc cgccaggccc gcgaggcctc gccgtccacc cagaagaggc cgccgccgcc
     5581 tgaggccagg ccgcggatgg aggcccctcc gggcggggcg agccactccg ggacgtcccg
     5641 gacgccgagg atcgtcaggg agaggcggaa gtccgcccag gcctccgcaa ggccgatccc
     5701 gtaggctttc ctgaaggcct tcgcgaagcc gcgcctgtcg ggatccaggg agaggctcgg
     5761 ggcgccaaag accatcgccc tccagagtcc ggcgtaggcc tccatgccgt agcgctcctg
     5821 gaggtaggcg ctgaagaggc cgccgtactc gtagtagacg gagccgcccg ggtaatcgtc
     5881 ccagacgccc gtcgcctccg tcggggactt gaagcggttc tccaggatgt cctggcggag
     5941 gcgctgccgg accagggggt cggcggcccg gccgccttcg ccgggggcgc tctcgaagct
     6001 gaccgtcacg ccctcgacca tgaagagggg ggcggtgaag agcgccggct tcatgtagga
     6061 gccgaagacg ccggagagga aggcggcgaa gggcgtggtc acctgcaggg agaccgcgtg
     6121 ggcgagctcg tggaggaaga gcgcccggaa gttgtcgcgg aaggccgtcc agcccatcgg
     6181 gaggggcgtg tcgaagagga cgatgtggaa gtagggcagg ggattcatga agccgttgaa
     6241 ggtggcgatg tccggcgtga ccaggacggg aacccgccgg ggcaggccgg actcgagctt
     6301 gcccgcgacc tcgtcgtaga cggactcggc gaaggaggcc aggcgcaggg cgctcggctc
     6361 agaggccttg gggaagatga tgtcgaagtg ctcggtcttg acgacgtaga gccgctggcc
     6421 gggggccacg agggggcctg cgccggccgg ccggggaggg aggagggcgg ccagggccag
     6481 gacaaggagg ggcgcgagac gaggagtgcg gcgcatgccg ggagcatgga ggaaagccgc
     6541 cggcggtgtc catgggggga agcccgcgcg gtaccgctcg cgtccggcgc cgccgcggcc
     6601 gcaccttcct cgcctagctc cgcgcctccg cgaggaaggc gaggatcccc tccttcccct
     6661 cgatgagccg cgtggctagg ccgtgctcct cggccttgcg gaggttgtcg gacaggtcgt
     6721 ccaggaagag gaggtcgcgg gcggccaggc cttcgcgcgc cgcgaggctt tcaaggacgg
     6781 cctcccagta gccgtcctcc gccttcacgt gtcctaggtc gcaggagaag aaggcgccgt
     6841 cgaagctctc ctccacggag agccactcga cgaggcgcgc ctttctccgg ggattctggt
     6901 tcgaggcgac gtagcaggag agccccgagg cccggagctc ccggacgccc tcaaggaggg
     6961 cgaggtcgac gaagcgctcc tcgaaggcgt actgggccgc taggtagtcg gcggccgagc
     7021 ccgggaagcc catctccgcg aggaagggcg cgagagcgag ctcggggtcg agctcgccct
     7081 tgtcgcagcg gaggttcgtc ccgctcgagt agaagcgccc gatggcctcc cgcgggcttt
     7141 cgcgccccgc gagggccagg gtctcgtaga agtgcgcccc gtggtggagg aggacgccgt
     7201 cgatgtcgaa gaggactgcc ctggtccccg gccctaggcc catgcgcccc tcctttcctc
     7261 ccactccctt gcgaggatgg cgaagcgtag gtcgtcggcc cagacgccgt ccacgaggac
     7321 gctctccagg aagagcccct cgcggcggaa gcccaggccc gaaaggaggg ccgccgaggc
     7381 gaggttctta gggtcgaggg aggccacggc ccggcgcgcg cgaagctcca ggaagaggaa
     7441 gtcgaggagg cgggcgaccg cctccttccc gtagcccttc ccctgccggg ccggcgcgat
     7501 cgtgtagccg atctccgcct ccaggccttc ggtcccgtag aggcgcgcgc ccaggtcccc
     7561 gatgagccct ccctccagga agaccccgag ctggctccag ccgccgcccg gggcgaaggc
     7621 cgccgggtag gcccggatga aggcctccgc ctccgcgagg cccgcggggc ggaaggactg
     7681 gaagcgctgg acctccggga gggagcggta ggcgaagagg gcgggagcgt cg
//
LOCUS       DAOOSJ010000125         7707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_5921_length_7707_cov_5.302535, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000125 DAOOSJ010000000
VERSION     DAOOSJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5921_length_7707_cov_5.302535"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1058)
                     /locus_tag="PLG14_07320"
     CDS             complement(198..1058)
                     /locus_tag="PLG14_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48985.1"
                     /translation="MGGQRYQSPNVQWDFGVVQGLAKRDGENLVEAFILYRGRLDLHD
                     TSLPDGRFADMRGLFSTSAIAGAAYLGVTRDSRRVMSGAAAEASAELSPSFANGSLGD
                     SDFFRLNLQAEGYLPLASFGRPSDPSLNLFSLYLAGFASLNHAEGAAIPIYVLQGFGG
                     REEHKSLGGSVRGYPSRGFDSGSKAVASAELRALGPALFGVPWFMPLVSAFCDVGYYS
                     GLPGATTAAYSGSRGAFYSAGFGLQADIVDFAYLGFYAGWKFPGDDPVYERYGETDPF
                     FVAFAFRLHF"
     gene            1484..2212
                     /locus_tag="PLG14_07325"
     CDS             1484..2212
                     /locus_tag="PLG14_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOX48986.1"
                     /translation="MRIAIDTHAHSVASGHAYSTIDELALAARRNGLKGFVLTDHGPA
                     MPGTTHPYHFGNLRVVPERLRGVRFYKGIEANVLDAEGRLDLDDRNLARLDFVLAGLH
                     EICLPPGDAEFNTLCLVRALESRYVDAVAHPGNPAFPIDARAVVLAAFANGKAVELNE
                     GSFRVRKGSEENCLAVARLCAESGTLVVAGTDTHYKDHIGRFDRVLGLLKEAGVPREL
                     VVNRSTRSFEAFLARRAEEKRGAR"
     gene            complement(2238..3983)
                     /locus_tag="PLG14_07330"
     CDS             complement(2238..3983)
                     /locus_tag="PLG14_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX48987.1"
                     /translation="MERARRSPSLFSIFAAALSAATLLPLVATLSLWSFVYEGNATRS
                     LGLAQISGALDGMAAFLESSFPTVDLEEDREALAKARRLLNGPIASIRVGPSEPAAAA
                     AALSAFLGTDELRCEAEEMTERGGRKVGKRNGDGTWTLSDQGWVARAAAAWDGATEEE
                     RRRLANGRVFVRAERDISKAVLRVGREGYVWAITAALAPDLPCYEFFHPQIESVDVSG
                     LFNESGERVGAEIAGLRGLLGTARASETIRYDYAWKNPGDARERRKIVLLRYLERQRI
                     VACAGLYEDEYFLPARTAQRLFIAMVLAIGCITLGLSLGIILRINRSLRDLTRFSASA
                     GAAGPSGSGAIEPFRSGIRELDVLSRTMVDMEGQILSRQRAMERELGEKNALLREVHH
                     RVKNNLAVLASMVSLQTSRTDSPEARAALERVEGRVSSMAIAYQQLVESDEFASIPFD
                     EYLRSLLTYYQSSGGPGRAPVARSEFLEPCRIGLEAAVPLGLAAQELISNAYAHGAPA
                     GGGAAVAVRLAKDGRRAVLEVADNGGGLAEGYEERTGLLLVRALAEQMGAEFSLESPA
                     APLGGTRASFAFGVE"
     assembly_gap    3998..4097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4105..4178)
                     /locus_tag="PLG14_07335"
     tRNA            complement(4105..4178)
                     /locus_tag="PLG14_07335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4142..4144),aa:Arg,seq:tct)
     gene            complement(4417..4489)
                     /locus_tag="PLG14_07340"
     tRNA            complement(4417..4489)
                     /locus_tag="PLG14_07340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4454..4456),aa:Pro,seq:tgg)
     gene            4785..6881
                     /locus_tag="PLG14_07345"
     CDS             4785..6881
                     /locus_tag="PLG14_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOX48988.1"
                     /translation="MAYSTDCIRNVAIAGHGGTGKTSLLEHLLFQGGAIGKPETIESG
                     KTVSDYGEDEISHKISVHAALAHLSLKDVKINLIDTPGSSDFVGEVIVALRSCETVLL
                     LVDGRSGVQIETMKLWRSLEQRQKPRALFVSRLDEERASFEKALDDVREKFHASPVAI
                     TIPVGEGSGYKGVVDVLNQKAYLRPASHDQKEAESPVPADMAAKLAEYRAKLFEAAAE
                     GDDELMEKYLGEGELTQEETVKGLKEALAAGRVVPAFAGAGVRNSGTAALLAFIADIA
                     PSPLSLAPETVKDSEGKEVEVRIDPSKPVSAFVIKTQIDQFSGRLSFVKVITGALGPD
                     MEVVNVRDSRKEKLGKLYTAQGKKLEEAGALSAGDIGIVIKAADLKTNDTISAWDRPV
                     AYRKLRLPTPVHTLAISAVNKKEEDKLNENLLKASEEDLTFQVHFNSETKETVIAGMG
                     ELQINMILDKIRNGPKIAAETRVPRVAYRETITKKASAEYTHKKQTGGHGQYARVVFD
                     VEPLERGVGYQFENKLFGQSVSKGFIPGIEKGIHQAMEAGVVAGYPVVDVKTAITDGK
                     EHPVDSSEMAFKIASRGAFREATKNAGAVLLEPVMNLKVFVEEKSLGDVMSDLSGRRG
                     RIAGQNPMGGGIVEIDAQVPQAELLRYAIDLRSMTSGTGSFEVEFDHYSPITGKIAED
                     VIKAAAAFRTHEEEEE"
     gene            complement(7034..>7707)
                     /locus_tag="PLG14_07350"
     CDS             complement(7034..>7707)
                     /locus_tag="PLG14_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48989.1"
                     /translation="GAPVAGRWFALDLGPDEGDALDEEELERARVRVLARRYGLLAKP
                     LLERELPLLGWGALFPAMRRMELAGELLYGRFFEGLDGPQFMSPEAFELWKGLGTLAA
                     GDEGPLWLSALDPASPAGFPFQERPGLLPPRALSARLCLSGGEVVASSLRSGKELRIG
                     PEPGDPAAAAIVSGIAPRSAGPRAARGRVVLETVNGQAAARSPYAALLAAAGFEADRG
                     KMTLW"
BASE COUNT         1327 a         2692 c         2529 g         1059 t
ORIGIN      
        1 cggccacgag ggcaacgagc gctgcgacca gatggtccag gtcgagatag ggaagctccg
       61 ctaggccccc ttcaagatcc tctgcgccca acgcgcctcg gccaaggggc tcggcttggc
      121 ctttgactca ggccgggccc cgaggcgcgg ctcggcgcgc ggcgcttggc tacggcggat
      181 cgaggccgat tcccgtccta gaagtgcagc cggaacgcga aagcgacgaa gaagggatcc
      241 gtctccccgt accgctcgta cacggggtcg tcgccgggaa acttccagcc cgcatagaag
      301 ccgagatacg cgaaatcgac gatgtccgcc tgcaggccga agcccgcgga atagaaggct
      361 ccgcgggagc ctgagtaggc cgcggtggtc gccccgggca ggcccgaata atagccgacg
      421 tcgcagaaag ccgagaccag aggcatgaac cagggcactc cgaacagggc gggacccaac
      481 gcccgcagct ccgcgctggc gaccgccttg ctgcccgaat cgaacccccg agagggatag
      541 cccctgaccg agccgccgag actcttgtgc tcctcgcggc cgccgaaacc ctgtagcaca
      601 tagatcggga tcgccgcgcc ttccgcgtgg ttaagactgg cgaagccggc caaatagagg
      661 ctgaacaggt tgagcgaagg atccgatggc cggccgaagc tcgccagggg caagtagccc
      721 tcggcctgga ggttcaggcg gaaaaagtcc gagtctccga ggctcccgtt cgcgaacgag
      781 gggctcaatt cggcggaagc ctcggccgcg gcccccgaca tgacccgcct tgagtcgcgc
      841 gtgacgccca agtaggccgc tcccgcgatg gccgaggtcg agaagagccc gcgcatgtcg
      901 gcgaagcggc cgtccggaag gctggtgtca tggaggtcca accgcccccg atagaggatg
      961 aaggcctcga ccagattctc gccgtccctc tttgccaagc cctggactac gccgaaatcc
     1021 cactggacat tgggagactg gtacctctgg ccgcccatgg gcgagctctc cgcagcctcg
     1081 acggatatcg gatccccggt gacggcgtcc ctgaggagga attcgtcctc gtagccggcg
     1141 ccaagcctca aggacagcgc cgtcgagcca atgcgggaat ccgcgagacc gtacctgaag
     1201 cggaaatccg ctcccgcggg caggggcatc ggcagggaaa ggagggagcc gttgaagcga
     1261 ggcccgatga actccacctc gagcttctga gcgccggcgg agacggatga aaccaagacg
     1321 agaagcgcga gcagatagga tcttcgcatg ccgatcctcc ccggaagtcc ttggatcaat
     1381 atagcgcccg gcccgccgcg ggacaagcca aggacgccct cgcggcggcc cacggctcct
     1441 cccctcgatc ccctggcccc tgccgtagta taactgggcc aacatgagaa tagcgatcga
     1501 cacgcacgcg cactccgtgg cctcgggcca cgcctactcc accatcgacg agctcgccct
     1561 cgcggcgagg aggaacggcc tcaagggctt cgtcctcacc gaccacggcc ccgccatgcc
     1621 cggcacgacc cacccctacc atttcggcaa cctcagggtc gtcccggagc gcctccgggg
     1681 cgtccgcttc tacaagggca tcgaggcgaa cgtgctcgac gccgagggcc ggctggacct
     1741 ggacgacagg aacctcgccc gcctcgactt cgtcctggcc ggcctccacg agatatgcct
     1801 ccccccgggg gacgccgagt tcaacaccct ctgcctcgtc cgggccctgg agagccgcta
     1861 cgtggacgcc gtcgcccatc ccggcaaccc ggccttcccg atcgacgcgc gggccgtggt
     1921 actggccgcc ttcgccaacg gcaaggccgt cgagctcaac gagggctcct tccgcgtccg
     1981 caagggaagc gaggagaact gcctggccgt cgcgcgcctc tgcgccgaga gcgggaccct
     2041 cgtcgtcgcg ggcaccgaca cccactacaa ggaccacatc ggccgcttcg accgggtcct
     2101 gggactcctc aaggaggcgg gcgtgcccag ggagctcgtc gtcaaccgct cgacgcggag
     2161 cttcgaggcc ttcctcgcga ggcgggcaga ggagaagcgc ggggcgcgtt agccggagcc
     2221 gacagaggag ccgtccgcta ctcgacgccg aaggcgaagc tggcccgcgt ccctcccagg
     2281 ggcgccgcgg ggctctccaa cgagaactcg gcgcccatct gctccgccag ggccctgacg
     2341 aggaggaggc ccgtccgctc ctcgtagccc tcggcgagcc ctccgccgtt gtccgccacc
     2401 tccaggacgg ctcgccttcc gtccttcgcg agcctcaccg cgaccgccgc gccgcccccg
     2461 gcgggcgcgc cgtgcgcgta ggcgtttgag atgagctcct gcgccgcgag gccgaggggc
     2521 acggccgcct cgagcccgat cctgcagggc tccaggaact cgctgcgcgc gacgggcgcc
     2581 cgccccggcc cgcccgaact ctggtaatac gttagcaggc tccgcaagta ctcgtcgaag
     2641 ggtatgctcg cgaactcgtc ggactcgacg agctgctggt aggcgatggc catggaggag
     2701 acgcgcccct ccaccctttc aagggcggcg cgggcctcgg gcgagtccgt cctcgacgtc
     2761 tgcagggaga ccatgctcgc gaggacggcg aggttgttct tgacgcggtg gtggacttcg
     2821 cggaggagcg cgttcttctc cccgagctcg cgctccatgg cgcgctgtcg ggagagtatc
     2881 tgcccctcca tgtccaccat cgtccgcgac agcacgtcga gctccctgat cccgctgcgg
     2941 aaaggctcga tcgcgcctga gcccgagggg ccggctgcgc cggccgacgc ggagaagcgc
     3001 gtcaggtccc ggagcgagcg gttgatcctg aggatgatgc ccagggagag gccgagggtg
     3061 atgcagccga tcgcgaggac catggcgatg aagagccgct gggccgtcct ggccggcagg
     3121 aagtactcgt cctcgtagag tcccgcgcag gcgacgatgc gctgcctctc gaggtagcgc
     3181 aggaggacga tcttcctgcg ctcccgcgcg tcgcccgggt tcttccacgc gtagtcgtag
     3241 cgtatcgtct ctgaggcgcg ggccgtcccg aggaggccgc ggagcccggc gatctcggcc
     3301 ccgacccgct cgccgctctc gttgaagagg ccgctcacgt ccacggactc gatctgggga
     3361 tggaagaact cgtagcaggg aaggtccggg gccagcgccg cggtgatggc ccagacgtag
     3421 ccttcccgcc ccacccgcag caccgccttg gagatgtccc tctccgcgcg gacgaagacg
     3481 cggccgttcg cgagccttcg ccgctcttcc tccgtcgcgc cgtcccaggc ggcggcggcc
     3541 cgcgccaccc agccctggtc cgagagggtc caggtgccgt ctccgttccg cttgccgacc
     3601 ttgcgccccc cgcgctccgt catttcctcg gcctcgcagc ggagctcgtc cgtcccgagg
     3661 aaggccgaga gggccgcggc ggccgcagcc ggctccgacg ggcccacgcg gatggaggcg
     3721 atgggaccgt tgaggaggcg cctggctttg gccagggcct cccggtcctc ctccagatcc
     3781 acggtcggaa acgaggactc gaggaaagcg gccatgccgt caagggcgcc ggatatctgc
     3841 gcgaggccga gggaacgcgt ggcgttgccc tcgtagacga aactccacag cgaaagcgtc
     3901 gcgacaaggg gcaggagggt cgccgccgaa agagccgcag caaaaatgga gaagaggctg
     3961 ggactccggc gcgcgcgttc catgtcggca attatcgnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnctt tgtccgggac cagaggggct cgaacccccg acctgctgct
     4141 tagaaggcag ctgctctatc ctgttgagct atgatccctt gaaggcgcga agcgcccttg
     4201 attgaagtct tgcccgaggg gccttgggct gtcaatgggt ggcgatttcc gggcatcgcc
     4261 gcgagccagg cgggaatggt gatggcgcgg acagaggagc aatcggcgag gattggcctg
     4321 gggaagcgga aaaaccggga cggggggatt tgcccggcgg cgcgcgacga cgtgccaagc
     4381 gccctcggtt caaatccccc ctcactcacc gtctcgtcgg gacgggggga tttgaacccc
     4441 cgatcttcag ctcccaaagc tgacgcctta gcccctaggc tacgtcccgg aagggcttac
     4501 cctaggccga gaccgctcca cctgtcaata cgaagggctc tatgcgcagg ccctccgcca
     4561 accttatcgg acggctggcg gagcgaaagc gggcaaacgc gccatttgcg ctttacgagg
     4621 cttttttcgg cattttgcgc tccggttcgt ggggatacgt gaagcgaggg cttgacacgc
     4681 tccggcgttt tttcttgaca gggggttttc gcggttctac gatacccgag cctcgttttc
     4741 agtataaccg ggacagccaa cttgacaccc gagaaggagc gattatggcg tattcgaccg
     4801 actgcatccg gaacgtggcc atagcgggcc acggcggcac gggcaagacg agcttgctcg
     4861 agcacctcct gttccagggg ggagccatcg gaaagccgga gacgatcgag tcgggcaaga
     4921 cggtcagcga ctatggagag gacgagatat cccacaagat ctccgtccac gcggcccttg
     4981 ctcacctgtc gctcaaggac gtcaagatca acctcatcga cacccccggc tcctccgact
     5041 tcgtgggcga ggtcatcgtg gccctgcgct cctgcgagac cgtcctcctc ctcgtcgacg
     5101 gccgctccgg cgtccagatc gagacgatga agctctggag gagcctcgag cagcgccaga
     5161 agccccgcgc cctcttcgtg tcccgcctgg acgaggagcg cgcctccttc gagaaggccc
     5221 tggacgacgt gcgcgagaag ttccacgcca gtcccgtggc catcaccatc cccgtcggcg
     5281 agggctcggg ctacaagggc gtggtcgacg tcctcaacca gaaagcctac ctgcggcccg
     5341 cctcgcacga ccagaaggag gccgagtcgc cggtccccgc ggacatggcc gcgaagctcg
     5401 ccgagtaccg cgccaagctc ttcgaggccg cggccgaagg cgacgacgag ctcatggaga
     5461 agtacctggg cgagggcgag ctcacgcagg aggagacggt gaagggcctc aaggaggccc
     5521 tggccgccgg ccgcgtcgtc cccgccttcg ccggcgcggg cgtcaggaac tccggcaccg
     5581 cggccctcct cgccttcatc gccgacatag cgccctcccc cctctccctc gcgcccgaga
     5641 ccgtgaagga ctccgagggc aaggaggtcg aggtcaggat cgatccctcg aagcccgtct
     5701 ccgccttcgt catcaagacc cagatcgacc agttctccgg ccgcctctcc ttcgtcaagg
     5761 taatcaccgg cgccctcggg ccggacatgg aagtcgtcaa cgtgcgcgac agccgcaagg
     5821 agaagctcgg gaagctctac accgcccagg gcaagaagct cgaggaggcc ggcgcgctct
     5881 ccgccggcga catcggcatc gtcatcaagg cggcggacct caagacgaac gacacgatct
     5941 ccgcctggga caggcccgtg gcctaccgga agctccgcct cccgaccccg gtccacaccc
     6001 tcgcgatctc cgcggtcaac aagaaggagg aggacaagct caacgagaac ctcctcaagg
     6061 cctcggagga ggacctcacc ttccaggtcc acttcaactc cgagaccaag gagacggtca
     6121 tcgccggcat gggcgagctc cagatcaaca tgatcctcga caagatccgc aacggcccca
     6181 agatcgcggc ggagacccgc gtcccccgcg tcgcctaccg cgagaccatc acgaagaagg
     6241 cgagcgccga gtacacccac aagaagcaga cgggaggcca cggccagtac gcgcgcgtcg
     6301 tcttcgacgt cgagcccctg gagcgcggcg tcggctacca gttcgagaac aagctcttcg
     6361 gccagtcggt ctccaagggc ttcatccccg gcatcgagaa gggcatccac caggccatgg
     6421 aggccggcgt cgtggcgggc taccccgtcg tcgacgtgaa gaccgccatc acggacggca
     6481 aggagcaccc ggtcgactcc tcggagatgg ccttcaagat cgcctcccgc ggcgccttcc
     6541 gcgaggccac caagaacgcc ggcgccgtcc tcctcgagcc cgtcatgaac ctcaaggtct
     6601 tcgtcgagga gaagagcctg ggcgacgtca tgtccgacct ctccggccgc cgcggccgca
     6661 tcgcgggcca gaaccccatg ggcggcggca tcgtcgagat cgacgcgcag gtgccgcagg
     6721 ccgagctcct gcgctacgcg atcgacctgc gctccatgac gagcggcacg ggcagcttcg
     6781 aggtcgagtt cgaccactac tccccgatca cgggcaagat cgccgaggac gtcatcaagg
     6841 cggccgcggc cttcaggacc cacgaggagg aggaggagta aggcctcccc gcgcggcgcg
     6901 cctcaaggcg agactccgca tggcgagacg ccgcgaggcg cgtcgcctcc cgccgtacgc
     6961 gaagggccgt cccggaaagg ggcggccctt ccttattcag gccaagggcc cctggccccg
     7021 cggacctggg ctcctaccag agcgtcattt tgcctctgtc cgcctcgaag cccgcggccg
     7081 cgaggagggc ggcgtaggga gagcgggcgg cggcctggcc gttcaccgtc tccaggacga
     7141 cgcggcccct ggcggccctc gggcccgccg agcgcggcgc gatgcccgac actatcgccg
     7201 cggccgccgg gtcgccgggc tccgggccta tcctgagctc cttgcccgag cggaggctcg
     7261 aggcgacgac ctctccgccc gagaggcaga gccgcgcgga aagggcccgc ggcggcagga
     7321 ggccgggcct ctcctggaag gggaagcccg cggggctcgc gggatcgagg gccgagagcc
     7381 acagcggccc ctcgtccccc gccgccaagg ttccgagccc cttccagagc tcgaaggcct
     7441 cgggactcat gaactgcggc ccgtccaggc cctcgaagaa gcggccgtag aggagctccc
     7501 ccgcgagctc catgcggcgc atcgcgggaa agagggctcc ccagccgagg aggggcagct
     7561 cccgctccag gaggggcttc gcgaggaggc cgtagcgccg cgcgaggacg cggacgcggg
     7621 cccgctccag ctcctcctcg tccagggcgt ctccctcgtc ggggcccagg tcgagggcga
     7681 accaccggcc cgcgaccgga gcgccgg
//
LOCUS       DAOOSJ010000126         7589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6040_length_7589_cov_5.753517, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000126 DAOOSJ010000000
VERSION     DAOOSJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6040_length_7589_cov_5.753517"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2155)
                     /gene="topA"
                     /locus_tag="PLG14_07355"
     CDS             complement(<1..2155)
                     /gene="topA"
                     /locus_tag="PLG14_07355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOX48990.1"
                     /translation="MGKSLVIVESPAKAKTINKYLGKDFVVKSSIGHIRDLPSSGGER
                     AASAKSPAEAAALARAATAKMSPKEKEAYRKAKAREQLVARMGIDPEHGWKARYEILP
                     GKEKVAEELRKLAKSADAVYLATDLDREGEAIAWHLREVLGGDPSKFRRVVFNEITQK
                     AIKAAFEEPGSIDMDRVNAQQARRFLDRVVGFMISPLLWKKVARGLSAGRVQSVAVRL
                     VVEREREIHAFVPDEYWELRADTATPKGQSLLLEAKKRGEEAFRPTGAAEAEAAVKAL
                     KGARFEVVQREDKPQRSNPPAPFITSTLQQAASTRLGFGVKKTMVMAQRLYEAGYITY
                     MRTDSTHLSDDAVAAVRSYIEAGYGAKYLPKSPNVYASKEGAQEAHEAIRPSNVAVAA
                     DGLADMERDAERLYALIWQQFVACQMEAALYTGTRVEVRAGEYALRARGRVVLFDGYT
                     RVLPPQSAGKEGEDDGILPEVKVGDVLSLRKLLPSQHFTSPPPRYTEASLVKELEKRG
                     IGRPSTYAAIISTIQERGYVRLEKRRLFAEKMGAIVTERLVENFADLMDYGFTAGMEE
                     SLDEVAEARKRWTEVLDEFYADFRKKLAKADSEKGGMRPNEPVEIDLPCPACSRPLQI
                     RTASTGVFLGCSGYGLPPKERCTKTLNLVSGDEIAEEDEEAEVRQLRARRRCPVCGTA
                     MDSYLIDSRRKLHVCGNNPDCRGYEIEEGEWKLKGY"
     gene            complement(2387..3394)
                     /gene="pdxA"
                     /locus_tag="PLG14_07360"
     CDS             complement(2387..3394)
                     /gene="pdxA"
                     /locus_tag="PLG14_07360"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOX48991.1"
                     /translation="MSLPVIALTLGDPAGIGPEIIVRACAEEAPARRIVYGDARVLEK
                     ALRAASSSLRLRAVSTPGAALFRPGELEVVDLANADPAGFEPGLVQASCGRAAFECVE
                     AAVGAALRGEVDAVDTAPVNKEALRAAGVPYIGHTEMLAGLTGASDPLTMFETRSLRI
                     FFLTRHVSLRQACDLVTEERVLDCVERCAAALGRLGLGGGELAVAGLNPHCGEHGLFG
                     DEEGRAVEPAVAKARARGLKVAGPIGADSVFHQAAAGRYAAVLSLYHDQGHIAAKTLD
                     FDRTVSLTLGLPFLRASVDHGTAFDIAWKGLASAAGMAESVRAAARYAIAYKESAGRR
                     A"
     gene            3517..3807
                     /locus_tag="PLG14_07365"
     CDS             3517..3807
                     /locus_tag="PLG14_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48992.1"
                     /translation="MKQRRAPLALAALLALLLCASCIPGDGKNSAEKPAGFWTGIWHG
                     WIAPVSLVAELFKPELSVYEEANKGFWYDLGFYMAVISGFGGLALSRKKRSS"
     gene            complement(3804..3965)
                     /locus_tag="PLG14_07370"
     CDS             complement(3804..3965)
                     /locus_tag="PLG14_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX48993.1"
                     /translation="MPTRVAIISNSALHEIYKADPRIFAIIIMNLARDIARRLRRMDE
                     MVCGAPPSP"
     gene            complement(4437..5282)
                     /gene="zupT"
                     /locus_tag="PLG14_07375"
     CDS             complement(4437..5282)
                     /gene="zupT"
                     /locus_tag="PLG14_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOX48994.1"
                     /translation="MPSSLELSAFGLTLFAGLSTGIGSLIALLAKRKTSRFLSMALGF
                     SAGVMVYVSLVEIFQEARLDLAAIWGPKGGYLGAVAGFFGGIALIALIDKLVPPVVNP
                     HESRAGEAATYEAADLRACPAEERDARRHGRLLRTGLFTAFVVALHNFPEGIATFLAA
                     LKSPRVGLAVALAIAIHNIPEGISISVPVYFATGSRRRAFWYSFASGLAEPAGALLGY
                     LFLRPYLGGPLFGIVTAGVAGIMVYISLDELLPAAREFGEPHFAMGGLVAGMAVMAGS
                     LVMLG"
     gene            5437..6414
                     /locus_tag="PLG14_07380"
     CDS             5437..6414
                     /locus_tag="PLG14_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOX48995.1"
                     /translation="MLDSYAIEGGALVHCPPEAARIFVFSSPDEAERRALVRDYGLDP
                     HELASALDPDELGRVDHDETRFSIILKRPCNFTGEDQLLFTVTSLGLFLEKDRLLVVA
                     SDSLGLFENRAVRRIDDARDALLKILYGTIAHFLGHLKVINMLSESIEKRINTSMENR
                     YLLDMFTLEKSLVYFVNGIDSNQMVLEKMRQAGERLGLSRPQVDILEEILIENRQCSK
                     QAEIYSNILTGLMDARGSVVNNNLSVLMKRLTVISVVFMPMNVLAGMGGMSEFSQWTR
                     GAPWWVAYSLFLAGLVMVGFLTYWVLKKTGLERSGGGRREPEGRRRRKA"
     gene            6411..7253
                     /locus_tag="PLG14_07385"
     CDS             6411..7253
                     /locus_tag="PLG14_07385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX48996.1"
                     /translation="MRKESGARAGAEPRAEIGDLAEAEVGSFCKCLEDWSAEFDDEGG
                     RKLAWYGRMREKGFRVKVAREEGKAVGMIQYGPAELAPIEGKGLYYVYCVWVHGYKEG
                     VGNRQRRGLGKALLKAAEEDARALGAKGLAAWGLRLPFFMRSSWFKRQGYEVADRDGM
                     AELVLKRFEPGAEAPRFVRPGAKPEEGRGRVRVAAFVNGWCPAQNLVYERARRAAEAF
                     PDKAEFVGVETEDKARLLECGFSDALFIDGKAVRTGPPPSYEKILGLVEKRLRKRGLL
                     GRGA"
BASE COUNT         1126 a         2569 c         2759 g         1135 t
ORIGIN      
        1 cgtagccctt gagcttccac tcgccctcct cgatctcgta gccgcggcag tcggggttgt
       61 tgccgcagac gtggagcttg cgcctcgagt cgatgaggta gctgtccatc gccgtgccgc
      121 agaccgggca gcggcggcgg gcgcggagct gccgcacctc ggcctcctcg tcctcctccg
      181 cgatctcgtc gccggagacg aggttcaggg tcttggtgca gcgctccttg ggcgggaggc
      241 cgtagcccga gcagcccagg aagacgccgg tggaggcggt gcggatctgc agggggcggg
      301 agcaggccgg gcagggcagg tcgatctcca cgggctcgtt gggcctcatg ccgcccttct
      361 ccgaatccgc cttggcgagc ttcttgcgga aatcggcgta gaactcgtcc aggacctccg
      421 tccagcgctt cctcgcctcg gccacctcgt ccagagactc ctccatgccc gcggtgaagc
      481 cgtagtccat gaggtcggcg aagttctcga ccaggcgctc cgtgacgatg gcgcccatct
      541 tctccgcgaa gagcctgcgc ttctccaggc gcacgtagcc gcgctcctgg atggtggaga
      601 tgatggcggc gtaggtcgag ggcctgccga tgccccgctt ctcgagctcc ttgacgaggc
      661 ttgcctcggt gtagcggggc ggggggctcg tgaagtgctg gctcgggagg agcttcctga
      721 gggagaggac gtcgcccacc ttgacctcgg ggaggatgcc gtcgtcctcg ccctccttgc
      781 ccgcggactg cgggggcagg acgcgggtgt agccgtcgaa gaggacgacc cggccccggg
      841 ctcgcaaggc atactcgccc gcccgcacct cgacgcgggt gcccgtgtag agcgccgcct
      901 ccatctggca ggcgacgaac tgctgccaga tcagggcgta gagccgctcc gcgtcgcgct
      961 ccatgtcggc caggccgtcg gccgccacgg ccacgttgct gggcctgatg gcctcgtggg
     1021 cctcctgcgc gccttccttg ctcgcgtaga cgttgggcga cttaggcagg tacttcgcgc
     1081 cgtagccggc ctcgatgtag gagcggacgg cggcgaccgc gtcgtcggag aggtgggtcg
     1141 agtccgtcct catataggtg atgtagccgg cctcgtagag gcgctgggcc ataaccatcg
     1201 tcttcttgac gccgaagccg aggcgcgtgc tcgcggcttg ctggagggtg gaggtgatga
     1261 agggggcggg agggttgctc ctctggggct tgtcctctcg ctggacgacc tcgaagcgcg
     1321 cgcccttgag cgccttcacg gcggcctccg cctccgcggc gcccgtcggg cggaaggcct
     1381 cctcgccccg cttcttcgcc tcgagtagga gggactggcc cttgggcgtc gccgtgtcgg
     1441 cgcggagctc ccagtactcg tccggcacga aggcgtggat ctcgcgctcg cgctccacga
     1501 ccaggcgcac ggcgacggac tggacgcggc cggcggagag gcccctcgcc accttcttcc
     1561 acaggagggg cgagatcatg aagccgacca cgcggtccag gaagcggcgg gcctgctggg
     1621 cgttcacgcg gtccatgtcg atggagcccg gctcctcgaa ggcggctttg atggccttct
     1681 gcgtgatctc gttgaagacg acgcgccgga acttggaggg atcgccgccc aggacctcgc
     1741 gaaggtgcca ggcgatggcc tctccctcgc ggtccaagtc cgtcgccagg tagacggcgt
     1801 cagccgactt ggcgagcttc cggagctcct cggccacctt ctccttgccc gggaggatct
     1861 cgtagcgggc cttccagccg tgctccgggt cgatgcccat gcgcgcgacg agctgctcgc
     1921 gggccttcgc cttccggtag gcctccttct ccttcgggct catcttggcc gtggcggccc
     1981 tggccagggc cgcggcctcg gcgggggact tcgcggaggc ggccctctcc ccgcccgacg
     2041 agggcaggtc ccggatgtgg ccgatcgagg acttcacgac gaagtccttg cccaggtact
     2101 tgtttatggt cttggccttg gccggcgact cgacgatgac gagggatttt cccatggctt
     2161 cctctggatg cggttcgggc ttcggtcggc gccggcctcg ggtcggcgcg gcgcgggcac
     2221 tgccccggcc ctgagccctc gggccggacc gattcgctac aagctagtaa atcacatttt
     2281 gcatgtcaag ggaaacgagg ggccgcgagg ccccggaaaa cccgtccggg agtttcgcgc
     2341 cgagggaagc gccgcgggcg gcgccgcggc ggcggcgcgg caggggctag gcgcggcgcc
     2401 cggccgactc cttgtaggcg atcgcgtatc gcgccgccgc ccgcaccgac tccgccatgc
     2461 ccgccgcgct cgcgagcccc ttccaggcta tgtcgaaggc ggtgccgtgg tcgacactcg
     2521 cgcggaggaa ggggaggccc agggtcaggg agacggtgcg gtcgaagtcg agggtcttcg
     2581 ccgcgatgtg gccctggtcg tggtagaggg ataggacggc cgcgtagcgg cccgccgcgg
     2641 cctggtggaa gaccgagtcg gcgccgatcg ggccggccac cttgaggccg cgggcgcggg
     2701 ccttcgccac ggcgggctcg acggccctcc cctcctcgtc gccgaagagg ccgtgctcgc
     2761 cgcagtgggg gttgaggccc gccacggcga gctcccctcc gccgaggccc aggcggccga
     2821 gggccgccgc gcagcgctcg acgcagtcga ggacgcgctc ctccgtcacg aggtcgcagg
     2881 cctgcctcaa ggagacgtgg cgcgtgagga agaagatccg caaggagcgg gtctcgaaca
     2941 tcgtcagggg gtcggaggcg cccgtgaggc ccgcgagcat ctcggtgtgg ccgatgtagg
     3001 gcacgccggc ggcccgcagg gcctccttgt tgacgggcgc cgtatccacg gcgtcgacct
     3061 ctccgcggag ggccgcgccg acggccgcct ccacgcactc gaaggcggcc ctgccgcagg
     3121 aggcctggac caggcccggc tcgaagcccg cggggtcggc gttcgcgagg tccacgacct
     3181 cgagctcgcc tgggcggaag agcgccgcgc cgggagtgga gacggccctg aggcggaggc
     3241 tcgaggaggc cgcgcggagc gccttctcga ggacgcgggc gtccccgtag acgatgcgcc
     3301 tcgcgggggc ctcctcggcg caggcgcgga cgattatctc ggggccgatc cccgcggggt
     3361 cgcccagggt cagcgcgatg acaggcaggc tcatggcggt cctcctcgcc ggaatcggcc
     3421 cggccgggcc aggccctgac gggaccgggg atcctggccc tccggatcct caaccgtgat
     3481 atactcgata cagacaccgc gatcaaggag cgaacgatga aacaaaggcg cgcgccgctg
     3541 gccctcgcgg ccctcctcgc ccttctcctc tgcgcatcct gcatccccgg cgacggcaag
     3601 aacagcgccg agaagcccgc gggcttctgg accggcatct ggcacggctg gatcgcgccc
     3661 gtctccctcg tcgccgagct cttcaagccc gagctctccg tctacgagga ggccaacaag
     3721 ggcttctggt acgaccttgg cttctacatg gccgtcatct ccggcttcgg cggactcgcc
     3781 ctctcgcgca agaaacggtc ttcctagggc gagggcggcg cgccgcagac catctcgtcc
     3841 atgcggcgga ggcggcgcgc gatgtcgcgg gccaggttca tgatgatgat ggcgaagatg
     3901 cgggggtcgg ccttgtagat ctcgtggagg gcgctgttgg agatgatggc gacgcgggtc
     3961 ggcaccaggg ccttgatcga ggcgacggcg ggcttggggt ccaggacctc gacctcgccg
     4021 aaggtctccc ctccccgagc tcgaggagct cctggtcccg cttgaggacc tcgacccggc
     4081 cctccagcac gaagtggagc cggccgttgg gctcgccctc cctgatgatc tcctcgccca
     4141 cggcgtagtc ggccgtctgc atgaagggcc tgagcctgtc gatgtcctcc ggcaggaggg
     4201 cgccgaagag ggagtatttc tgcaaggcgg cgacgtcgac catgggcttt cgcgcccctc
     4261 cgcaaagcgc ttcatccgac agtagcacgt acttgccggg gccgcaaggc gagccggggc
     4321 cgcaaggcga gccaggcgcg cacggcgagg gacggctgcg gggcggccag gcgggtacgc
     4381 ggagcgaggc tcggccgcgg cccgggcgcc gcccggaatc tccgggcgcg ccgccctcag
     4441 cccagcatga cgaggctgcc ggccatcacc gccatgcccg cgaccaggcc gcccatcgcg
     4501 aagtggggct cgccgaactc gcgcgcggcg ggcaggagct cgtccagtga gatgtagacc
     4561 atgatgccgg ccacgcctgc ggtgacgatg ccgaagaggg ggccgcccag gtagggcctg
     4621 aggaagaggt agccgaggag ggcgccggcg ggctcggcga ggccggaggc gaaggagtac
     4681 cagaaggcgc ggcgcctcga gcccgtcgcg aagtagacgg ggacggagat ggagatgccc
     4741 tccgggatat tgtggatggc gatggccagg gcgacggcga ggccgacccg cgggctcttc
     4801 agggccgcga ggaaggtggc gatgccttca ggaaagttgt ggagggcgac gacgaaggcg
     4861 gtgaacagcc ccgtccggag gaggcggccg tggcggcggg cgtcccgctc ctcggcgggg
     4921 caggcccgga ggtcggcggc ctcgtaggtc gcggcctcgc ccgctcgcga ttcgtggggg
     4981 ttcacgacgg ggggcacgag cttgtcgatg agggcgatga gggcgatgcc gccgaagaag
     5041 cccgcgacgg cgcccaggta gccgcccttc ggcccccaga tcgcggccag gtccaggcgc
     5101 gcctcctgga agatctccac gagggagacg tagaccatca cgcccgcgga gaagcccagg
     5161 gccatggaca ggaagcgcga ggtcttccgc ttcgcgagga gggcgatgag gctccctatg
     5221 ccggtcgaga ggccggcgaa gagggtgagg ccgaaagccg agagctcaag ggacgacggc
     5281 atcgggagga tcctccgaat agaggccggt ccgcggcctc caggcgagca tagcccaggt
     5341 ggcgcggttt cgggaagcgg cataattagc cttggctaac tttctttgcc ccgcggcccg
     5401 cgatcggcta gactggatac tgccaggagg ccgtccatgc tcgacagcta cgcgatcgaa
     5461 ggcggcgcgc tcgtccactg ccccccggaa gcggcccgga tcttcgtctt ctcctccccc
     5521 gacgaggcgg agaggcgcgc cctcgtgcgg gactacggcc tcgaccccca cgagctcgcc
     5581 tccgccctcg atcccgacga gctcggccgc gtagaccacg acgagacgcg tttctcgata
     5641 atcctgaaac gcccctgcaa cttcacggga gaggaccagc tcctcttcac ggtgacctcc
     5701 ctcggcctct tcctcgagaa ggaccgcctc cttgtcgtcg cgagcgatag cctgggcctc
     5761 ttcgagaacc gggcggtgcg caggatcgac gacgctcgcg acgccctcct caagatcctc
     5821 tacgggacca tcgcccactt cctgggccac ctaaaggtca tcaacatgct ctcggaatcc
     5881 atagagaagc gcatcaacac ctccatggag aaccgctacc tcctcgacat gttcaccctg
     5941 gagaagagcc tcgtctactt cgtgaacggc atcgactcga accagatggt cctggagaag
     6001 atgcgccagg cgggcgagcg gctcggcctc tcgcggcccc aggtcgacat cctggaggag
     6061 atcctcatcg agaaccgcca gtgctccaag caggcggaga tctactcgaa catcctcacg
     6121 ggcctcatgg acgcgcgcgg ctcggtcgtc aacaacaacc tctccgtcct catgaagcgc
     6181 ctcaccgtga tctccgtcgt cttcatgccg atgaacgtgc tcgcgggcat gggcggcatg
     6241 tcggaattct cgcagtggac ccgcggcgcg ccctggtggg tagcctactc cctcttcctc
     6301 gcgggcctcg tcatggtcgg tttcctgacc tattgggtgc tcaagaagac cggcctggag
     6361 cggagcggcg ggggacggcg agagcccgag gggcggagga ggaggaaggc atgaggaagg
     6421 agagcggcgc aagagcaggc gcggagcccc gcgcggagat cggcgacctt gcggaggccg
     6481 aggtcgggag cttctgcaag tgcctggagg actggtcggc cgagttcgac gacgagggcg
     6541 gccgcaagct ggcctggtac ggccggatgc gggagaaggg cttccgcgtg aaggtcgcga
     6601 gggaggaggg caaggccgtc ggcatgatcc agtacggccc cgccgagctc gcgccgatcg
     6661 aggggaaggg cctctactat gtctactgcg tctgggtcca cggctacaag gaaggcgtcg
     6721 ggaaccgcca gaggcggggc ctcgggaagg ctctcctaaa agcggcggag gaggacgccc
     6781 gggcgctcgg cgcgaagggc ctcgccgcct ggggcctcag gctccccttc ttcatgcgct
     6841 cctcctggtt caagcggcag ggctacgagg tcgccgaccg cgacgggatg gcggagctcg
     6901 tcctgaagcg cttcgagccg ggggccgagg cgccgcgctt cgtccggccc ggggccaagc
     6961 ccgaggaggg tcgcgggcgg gtgcgcgtgg ccgccttcgt gaacggctgg tgcccggcgc
     7021 agaacctggt ctacgagcgg gcgaggcgcg cggcggaggc cttcccggac aaggccgagt
     7081 tcgtcggcgt cgagacggag gacaaggcgc gcctcctcga atgcggcttc tccgatgccc
     7141 tcttcatcga cggcaaggcg gtgaggacgg ggccgccgcc ctcctacgag aagatcctcg
     7201 ggctcgtcga gaagcgcctc cggaagcggg gcctcctggg ccgcggcgcc taggctcgcc
     7261 cgcagggtcc aggcgcccag gtccgcggtg caatccaccc gcgggcgcgc ttcccgcggc
     7321 gccggccgcc gcagcgcccg cagggcagcc gcgcctcccc gcctcccgcg ggcgccgcgg
     7381 gaagcggaag cggcgcgcgc ggaaagggaa ccgccgttaa ttctatacgc agtaacgcgc
     7441 cacttgcttg acgacgccta acgcaccccg gtatgcttca ctccatattt cgagagggcg
     7501 cctagggttc cttctcgccc cgtgcgccct ccttaggacg cgcgccgggg cgggagcacg
     7561 gtccgagcgg cgccggccgg acttgcgcc
//
LOCUS       DAOOSJ010000127         7568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6064_length_7568_cov_6.475309, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000127 DAOOSJ010000000
VERSION     DAOOSJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6064_length_7568_cov_6.475309"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..948)
                     /locus_tag="PLG14_07390"
     CDS             complement(1..948)
                     /locus_tag="PLG14_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48997.1"
                     /translation="MNILYSMIPIALAYSAPLVIAALGGLFSERSGIVNIALEGIMMV
                     GGFTAASVTVALEPISPAAGYWGLLAGLAAGVVFSSLHAFTSINLKANQVISGTALNI
                     LAGGLTVYLAQILFHQQRTAAFTSGLVKVSVPGLKSIPLLGPLLFVNASPPFYIAVVL
                     VALSWFVVFKTPFGLRLRACGEHPQAAASMGIDVLRFRYVGVLLSGAFAGLAGGALVL
                     TQDIQYTAVSIHGSGFIAMACLIFGKWNPFGVLGAGIFFGLSQALSLYAKDIRFLAGI
                     PSEFFYLLPYALTILALLVFAGKAVGPKAAGEIYDAGKR"
     gene            complement(945..2042)
                     /locus_tag="PLG14_07395"
     CDS             complement(945..2042)
                     /locus_tag="PLG14_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX48998.1"
                     /translation="MPKHKKQSILVPIVAILFGFALGAVVLVLTGRNPGSMFLAMFQG
                     ATGINVKAGTFNPRYIGEFVIQSMPIMLSGLSVAFAFRTGLFNIGAEGQLIAGSVAAT
                     AVALWVQAPPGVHAVLVLLAAAAAGAFWGAIPGWLKARFNVHEVVITIMMNYLALFLN
                     NWMVLYVFGSKDRIKTALFPASALLKDDALAALTNGSRLNWGFVPVLLSVLLSWFIIE
                     KTTFGYSLRAVGFNKEGARYAGMKVERNIVLSMAIAGAFAGLAGAVITTGTFNYGRFL
                     PGAEGYGMDGIAVALVGGNAAFGVFFSALLFGMLKAAQPLMQSQGVPKEIASIIQASI
                     VLFIAMRLGIETLISRLKRKSPETLGEEAGT"
     gene            complement(2047..3567)
                     /locus_tag="PLG14_07400"
     CDS             complement(2047..3567)
                     /locus_tag="PLG14_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX48999.1"
                     /translation="MAFVIEMLDIDKSFPGIRANDHVTLQVEERSIHALLGENGAGKS
                     TLMSILFGLYQPDAGTIRVRGKDVRITDPNVANELGIGMVHQHFKLVHNFTVTENIIL
                     GLEPRKGLVVDARKAERRVEELSRLYGLNVDPRARIEDISVGMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIHELMGIMRRLVGEGKTVLLITHKLKEIKEVADSCTVLRRGRL
                     IGTVPVAETSEEKMAEMMVGREVNFHIAKAPANPGEVVLRLEGLNVRNPKGLLGVKDV
                     SLDVRAGEILGLCGIDGNGQTELIKAITGLAPAESGRILLGGKDIARMPVKRRLDLGL
                     GHIPEDRHRHGLILDFRLDENLVVHDYMRAPYARAGILDSRAIGAKAGKLIEEFDVRC
                     GQGGATPTRSMSGGNQQKAIVAREIDRSPGALVVAQPTRGLDVGAIEYIHKRLVAERD
                     NGKAILLVSLELDEILDLSDRIAVIYGGRIVGLLDAKSTNENELGLLMSGSSNKVA"
     gene            complement(3759..4763)
                     /locus_tag="PLG14_07405"
     CDS             complement(3759..4763)
                     /locus_tag="PLG14_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX49000.1"
                     /translation="MKKSIAIMLLGLVAAAALVAAPIKVGLVTDVGGIDDKSFNQGTW
                     EGIRLFAKENKLKEGTDVKYLQSAADADYIPNLSTFADEGLDLIVAPGFLFEEAMKTV
                     ADQYPKQKLLIIDSVVAKPNVASAVFAANEGSFLAGVAAGLKAKADKKDTIGYVGGMK
                     FPLIENFQAGFEQGVKAVLPGAKILVDYSGSFTDAGIGQALAAKQYNAGAYIIFQVAG
                     GCGNGVIKEAKERSAKGDIRWAIGVDKDQYADGVYASGKSAILTSMMKRVDVASHDVA
                     VMVRDGKFPGGTTLVFDLKSKGVGLPEKNPNLSAAIMKEVEKFTAQITSGKLKVNSVQ
                     "
     gene            complement(4948..5622)
                     /gene="deoC"
                     /locus_tag="PLG14_07410"
     CDS             complement(4948..5622)
                     /gene="deoC"
                     /locus_tag="PLG14_07410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOX49001.1"
                     /translation="MPKEWTKASLAKTIDHTLLKAIGTADQVRELCAEARKFGFASVC
                     VNPVWVPLCAKELAGTGVMVCTVIGFPLGANQSEIKAAEAKLAVAQGAHEVDMVINIG
                     AAKAGDWKTVEEDIGAVVAAAAGKAKVKVIIETCYLSDAEKAKACEAAQRAGADFVKT
                     STGFGTGGATPEDVKLMKKAVGGKLQVKASGGVRTYHDAILMLDAGADRIGASSGVAI
                     IAELPE"
     gene            complement(5638..6192)
                     /locus_tag="PLG14_07415"
     CDS             complement(5638..6192)
                     /locus_tag="PLG14_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49002.1"
                     /translation="MDLDNMIRLKSMHLAPYIQMATALIGKRRRSGGNMFRHQLDTMA
                     ILMDYGYIDSVLLKASVIHDLLEDVPDFDRDLILEADEESPEVYKLVLEVTRRPIETK
                     PEFLGRILEYGSHRARVLKSADRISNMISLGYVTDVEFIRRYTDETEIHVYPIAALSD
                     ERMLSELKELVATRREYLARCFEI"
     gene            complement(6282..>7568)
                     /locus_tag="PLG14_07420"
     CDS             complement(6282..>7568)
                     /locus_tag="PLG14_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX49003.1"
                     /translation="DVLDTWFSSWLWPFSTLGWPKDTADFRKYYPTSSLVTAYDIIFF
                     WVARMIMAGVEFTGKSPFKDVYIHGLVRDKQGRKMSKSLGNGLDPLEIVDEYGADALK
                     FTLAYNCAAGQDVCVDKESFKMGSKFANKVWNASRYILMNLEGRELVQAPARNDVDRW
                     ILHRLDAAAAASRSALEGYRYNEAAQAAYAYFWDDFCDWYIEATKLSLRSGDEAEKNR
                     ATTVLLEVLEESLRLLHPFLPFVTEEIYRMLPNAKGRLIVQAYPEPEAARASPGLAER
                     FESLRELVRLVRTLRPEFQIPPETRIRLAVKPEAGFAGLAFLKDNAALVGLLVNGPAP
                     EFVAAKPTGAVALVGKGFEAYAFVKESVDAAKLVEKFRKDIEKDGQYLERTRAKLANA
                     AFVASAPPEVVAREREKLEETERRAAKLAQYVEELK"
BASE COUNT         1176 a         2321 c         2800 g         1271 t
ORIGIN      
        1 tcatcgcttg cccgcgtcgt agatctcgcc cgcggccttg ggcccgaccg ccttcccggc
       61 gaagacgagg agggccagga tcgtaagcgc gtaggggagg agatagaaga actccgaggg
      121 gatgcccgcc aggaagcgga tgtccttggc gtagagcgag agggcctgcg agagcccgaa
      181 gaagatgccc gcgcccagga cgccgaaggg gttccacttg ccgaagatga ggcaggccat
      241 cgcgatgaag cccgagccgt ggatggagac cgcggtgtac tggatgtcct gggtcaggac
      301 cagggccccg ccagcgaggc cggcgaaggc gcccgagagg aggacgccga cgtagcggaa
      361 gcggaggacg tcgatgccca tggaggccgc ggcctgggga tgctcgccgc aggccctgag
      421 cctgaggccg aagggggtct tgaagacgac gaaccaggag agggccacga ggacgacggc
      481 gatgtagaag ggcggggagg cgttgacgaa gaggaggggc ccgaggaggg gtatcgactt
      541 gagcccgggt acggacacct tgacgaggcc gctcgtgaag gcggccgtgc gctgctggtg
      601 gaagaggatc tgcgcgaggt agaccgtgag gccgcccgcg aggatgttga gggccgtgcc
      661 cgagatgacc tggttggcct tcaggttgat gctcgtgaag gcgtggagcg aggagaagac
      721 gacgcccgcg gccaggccgg ccaggagtcc ccagtagccg gcggcggggc tgatcggctc
      781 cagggccacg gtgacggagg ccgcggtgaa gccgcccacc atcatgatgc cctccagcgc
      841 gatgttgacg atgccggagc gctcggagaa gaggccgccc agggcggcga tgacgagggg
      901 ggccgagtag gccagggcaa tggggatcat cgagtagagt atgttcatgt gccggcctcc
      961 tcgccgagcg tctcgggact cttccgcttg aggcgggaga tgagcgtctc gatgcccagg
     1021 cgcatcgcga tgaagaggac gatcgaggcc tggatgatgc tcgcgatctc cttgggcacg
     1081 ccctgggact gcatgagggg ctgggccgcc ttgagcatcc cgaagagcag ggcggagaag
     1141 aagacgccga aggcggcgtt gccgccgacc agggcgaccg ctatgccgtc catcccgtag
     1201 ccctcggcgc cgggaaggaa gcggccgtag ttgaaggtgc cggtcgtgat gaccgcgccc
     1261 gcgaggcccg cgaaggcccc cgcgatcgcc atggagagga cgatgttgcg ctcgaccttc
     1321 atgcccgcgt agcgcgcgcc ctccttgttg aagccgacgg cgcggaggct gtagccgaag
     1381 gtcgtcttct cgatgatgaa ccaggagagg agcaccgaga gcaggacggg cacgaagccc
     1441 cagttgagcc gcgagccgtt cgtgagcgcg gcgagcgcgt cgtccttgag gagggcggag
     1501 gcggggaaga gggccgtctt gatccggtcc ttgctcccga agacgtagag caccatccag
     1561 ttgttcagga agagcgccag gtagttcatc atgatcgtga tgacgacctc gtggacgttg
     1621 aagcgggcct tgagccagcc ggggatggcg ccccagaagg ctccggctgc cgcggccgcg
     1681 aggaggacga ggacggcgtg gacgccggga ggcgcctgga cccagagggc cacggcggtg
     1741 gccgccaccg agccggcgat gagctggccc tcggccccga tgttgaagag gccggtccgg
     1801 aaggcgaagg ccacggagag gccggacagc atgatgggca tcgactggat gacgaactcg
     1861 ccgatgtagc gggggttgaa ggtccccgcc ttcacgttga ttccggtcgc tccctggaac
     1921 atcgccagga acatcgagcc cggattgcgg cccgtgagga cgaggacgac ggcgcccagg
     1981 gcgaagccga agaggatcgc cacgatcggc accaggatgc tttgcttctt gtgcttcggc
     2041 atagccttac gccaccttgt tggacgagcc cgacatgagg agcccgagct cgttctcgtt
     2101 ggtcgacttc gcgtcgagga ggccgacgat ccggccgccg tagatgaccg cgatgcggtc
     2161 ggagaggtcc aggatctcgt cgagctccag ggagacgagg aggatggcct tgccgttgtc
     2221 ccgctccgcg acgaggcgct tgtggatgta ctcgatcgcg ccgacgtcca ggccccgcgt
     2281 cggctgcgcg acgacgaggg ctccggggga gcggtcgatc tcgcgggcga cgatggcctt
     2341 ctgctggttg ccgccggaca tcgagcgcgt cggcgtggcg ccgccctggc cgcagcggac
     2401 gtcgaactcc tcgatgagct tgccggcctt ggcgccgatg gcccgcgagt cgaggatgcc
     2461 ggccctcgcg tagggcgcgc gcatgtagtc gtggacgacc aggttctcgt caaggcggaa
     2521 gtccaggatg aggccgtgcc ggtggcggtc ctccgggatg tggcccaggc ccaggtccag
     2581 gcggcgcttc acgggcatcc tcgcgatgtc cttgccgccg aggaggatcc gccccgactc
     2641 cgcgggcgcg aggccggtga tggccttgat gagctccgtc tggccgttgc cgtcgatgcc
     2701 gcagaggccc aggatctcgc cggcccggac gtccaggctc acgtccttca cgcccaggag
     2761 ccccttgggg ttgcggacgt tgagcccctc gagcctgagg acgacctcgc cgggattggc
     2821 gggggccttc gcgatgtgga agttgacctc gcggccgacc atcatctccg ccatcttctc
     2881 ctcggaggtc tcggcgacgg gcacggtgcc gatgaggcgg ccgcgcctca ggaccgtgca
     2941 tgagtcggcg acctccttga tctccttgag cttgtgggtg atgaggagga ccgtcttccc
     3001 ctcgcccacg aggcgccgca tgatgcccat gagctcgtgg atctcctggg gcgtgagcac
     3061 ggccgtcggc tcgtcgaaga tgaggatctc cgcgtcgcgg tagagcatct tgaggatctc
     3121 cacgcgctgc tgcatgccga ccgagatgtc ctcgatcctg gcgcgggggt cgacgttgag
     3181 gccgtagagc ctggagagct cctcgacccg ccgctccgcc ttgcgggcgt cgacgacgag
     3241 gcccttgcgc ggctccaggc ccaggatgat gttctccgtg acggtgaagt tgtggacgag
     3301 cttgaagtgc tggtgcacca tgccgatccc gagctcgttg gcgacattcg ggtccgtgat
     3361 gcgcacatcc ttcccccgca cccgtatcgt gcccgcgtcg ggctggtaca ggccgaagag
     3421 gatgctcatc agcgtcgact tcccggcgcc gttctccccg aggagggcgt ggatggaccg
     3481 ctcctccacc tgcagggtga cgtggtcgtt ggcgcggatg cccgggaagg acttgtctat
     3541 gtcgagcatc tcgatgacaa aagccacgaa ggcctcctag tttccaggtt ttgggcaagt
     3601 atagcaccga tcccggcgaa gggaagcggc gctcgccggt cccgtcgccc gcgaggggcc
     3661 ccggaaccgg gggaaaagcg agcccccggc tcgctgccgg gggctctccg cgcccgagga
     3721 cgggcgacgg gccggctcag ggccggcggc cttgggtctt actggacgga gttgaccttg
     3781 agcttgcccg aggtgatctg ggcggtgaac ttctccactt ccttcatgat ggcggcggag
     3841 aggttggggt tcttctcggg gaggccgacg cccttcgact tgaggtcgaa gacgagggtc
     3901 gtgccgccgg ggaacttgcc gtcgcggacc atgacggcca cgtcgtggga ggcgacgtcg
     3961 acgcgcttca tcatggaggt gaggatggcg gacttgccgg aggcgtagac gccgtcggcg
     4021 tactggtcct tgtcgacgcc gatggcccag cggatgtcgc ccttcgcgga gcgctccttg
     4081 gcctccttga tgacgccgtt gccgcagccg cccgcgacct ggaagatgat gtaggcgccc
     4141 gcgttgtact gcttcgcggc gagggcctgg ccgatgccgg cgtcggtgaa ggagccggag
     4201 tagtcgacga ggatcttggc gccggggagg acggccttga cgccctgctc gaatccggcc
     4261 tggaagttct cgatgagggg gaacttcatg ccgcccacgt agccgatggt gtccttcttg
     4321 tcggccttgg ccttgaggcc ggcggcgacg cccgcgagga aggagccctc gttggcggcg
     4381 aagacggcgc tggcgacgtt cggcttggcg acgacggagt cgatgatgag gagcttctgc
     4441 ttcggatact ggtcggcgac ggtcttcatc gcctcctcga agaggaagcc gggggcgacg
     4501 atgaggtcga ggccctcgtc ggcgaaggtg gagaggttcg ggatgtagtc ggcgtccgcg
     4561 gcggactgga gatacttgac gtcggtccct tccttgagct tgttctcctt ggcgaagagc
     4621 ctgatgccct cccaggtgcc ctggttgaag gacttgtcgt cgattccgcc gacgtcggtg
     4681 acgagaccga ccttgatggg cgcggccacg agggcggcgg cggctacgag acccagaagc
     4741 atgatggcga ttgacttctt catcaacatt ctccttcacg cagtattgtg ctagtgcgtt
     4801 ttgaaatcat aggcccggtt ttccggaaat gcaatcggat tctgtataca aatgcgcgcc
     4861 gcgggccgcg cgccggcgcc gggcaaagcc cgtcggcgcc gaagcgccgg ggcgcttcgg
     4921 cgatcgtccg cgggccggcg cggagcctta ctccggaagc tccgcgatga tcgccacgcc
     4981 ggagctcgcc ccaatgcggt ccgcgccggc gtcgagcatg aggatggcgt cgtggtaggt
     5041 gcggacgccg cccgaggcct tcacctggag cttgccgccg acggccttct tcatgagctt
     5101 gacgtcctcg ggggtggcgc cgcccgtgcc gaagccggtg ctggtcttca cgaagtcggc
     5161 gccggcccgc tgggcggcct cgcaggcctt ggccttctcg gcgtcggaga ggtagcaggt
     5221 ctcgatgatg accttgacct tcgccttgcc ggcggcggcg gccacgacgg ccccgatgtc
     5281 ctcctcgacc gtcttccagt cgccggcctt ggccgcgccg atgttgatca ccatgtccac
     5341 ctcgtgggcg ccctgggcga cggcgagctt ggcctcggcg gccttgatct cgctctggtt
     5401 cgcgcccagg gggaagccga tcacggtgca gaccatgacg ccggtgcccg cgagctcctt
     5461 ggcgcagagg ggcacccaga cgggattgac gcagacggag gcgaagccga acttgcgggc
     5521 ttcggcgcag agctcgcgga cctggtcggc cgtgccgatg gccttgagga gggtgtggtc
     5581 gatggtcttc gcgaggctcg ccttggtcca ttccttgggc atgttttgct cctttgacta
     5641 gatctcgaaa cagcgcgcca ggtactcgcg gcgcgtcgcc acgagctcct tgagctccga
     5701 gagcatccgc tcgtcggaga gggcggctat ggggtacacg tggatctcgg tctcgtcggt
     5761 gtagcggcgg atgaattcca cgtcggtgac gtagccgagg cttatcatat tggagatcct
     5821 gtcggccgac ttgaggaccc gcgcgcggtg ggagccgtac tccaggatgc ggcccaggaa
     5881 ctcgggcttg gtctcgatgg gacgccgcgt cacctcgagg acgagcttat agacctcggg
     5941 ggactcctcg tcggcctcga ggatgaggtc gcggtcgaag tccggcacgt cctcgaggag
     6001 gtcgtggatc acggaggcct tgaggaggac cgagtcgatg tagccgtagt ccatgaggat
     6061 ggccatcgtg tccagctggt gccggaacat gttgccgccc gagcgccgcc gcttcccgat
     6121 cagggccgtc gccatctgga tgtagggggc gaggtgcatg ctcttgaggc ggatcatgtt
     6181 gtccaggtcc atgccgccct cgcccccgtc ggtcctgtag ccgcccgcgg gcgaggagcc
     6241 cgggtcgggg gcgcgccgcc tggccgagcc cccgtcgatc ctcacttgag ctcctccacg
     6301 tactgggcga gcttggcggc gcgccgctcg gtctcctcga gcttctcgcg ctcccgggcg
     6361 accacctcgg gcggcgccga ggcgacgaag gccgcgttgg cgagcttcgc cctggtccgc
     6421 tccaggtact ggccgtcctt ctcgatgtcc ttcctgaatt tctccacgag cttggccgcg
     6481 tcgaccgact ccttcacgaa ggcgtaggcc tcgaagccct tgccgacgag ggcgacggcc
     6541 ccggtgggct tcgcggcgac gaactcgggc gcggggccgt tcacgaggag gccgaccagg
     6601 gcggcgttgt ccttgaggaa ggcgaggccc gcgaagccgg cctccggctt gaccgcgagg
     6661 cggatcctgg tctcgggagg gatctggaat tcggggcgca gggtcctgac caggcggacg
     6721 agctcgcgca gggactcgaa gcgctcggcg agcccggggg aggcgcgggc cgcctccggc
     6781 tcggggtagg cctggacgat gagccggccc ttcgcgttcg ggagcatccg gtagatctcc
     6841 tcggtgacga aggggaggaa gggatggagg aggcgcaggg actcctccag gacctcgagg
     6901 aggacggtcg tcgcgcggtt cttctccgcc tcgtcgcccg agcgcaggga gagcttggtc
     6961 gcctcgatgt accagtcgca gaagtcgtcc cagaagtagg cgtaggcggc ctgggcggcc
     7021 tcgttgtagc ggtagccctc cagggccgag cgggaggcgg ccgcggcggc gtccaggcgg
     7081 tggaggatcc agcggtcgac atcgttccgg gcgggcgcct ggacgagctc gcggccctcc
     7141 aggttcatga ggatgtagcg gctcgcgttc cagaccttgt tcgcgaactt ggagcccatc
     7201 ttgaaggact ccttgtcaac gcagacgtcc tggccggcgg cgcagttgta ggcgagggtg
     7261 aacttgaggg cgtcggcgcc gtactcgtcg acgatctcca gggggtccag gccgttcccg
     7321 agggacttgg acatcttgcg gccctgcttg tcccgcacga ggccgtggat gtagacgtcc
     7381 ttgaaggggc tcttcccggt gaactccacg ccggccatga tcatcctggc cacccagaag
     7441 aagatgatgt cgtaggcggt gaccagggag gaggtcgggt agtacttgcg gaagtcggcc
     7501 gtgtccttgg gccagcccag ggtgctgaag ggccagagcc agctggagaa ccaggtgtcc
     7561 aggacgtc
//
LOCUS       DAOOSJ010000128         7537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6099_length_7537_cov_5.794440, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000128 DAOOSJ010000000
VERSION     DAOOSJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6099_length_7537_cov_5.794440"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLG14_07425"
     CDS             complement(<1..1082)
                     /locus_tag="PLG14_07425"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOX49004.1"
                     /translation="MSWESTAEYYRILNEEVARRAGGLSSARLYLASVDFSGYASRMR
                     EGRWDEIRLALVGEALALKRAGAEAVLVATNTMHRFAPELEAATGLPLLHIADAAGAA
                     ARARGAARVGLLGTRYTMEGDFYSGRLRERFGIETLVPPEAERREVDAVIFDELCRGV
                     FSAPSRARLREVARGLEERGAEGIVLGCTELPLAMDDGDLGVPYFDTTRLHALAAVDF
                     MLDAAAGPVSGIASPDAAHAGSGGAAAASQAASQAAAGKPEDPSVAAMWAAYLGSLSG
                     EARARAERRGHTAWHFCADKANADELGALALAGRKRGTATSLRALERGGEPAPAAGDL
                     SVIVDWEGRALCVIETERIAIRRFDDV"
     gene            1280..1414
                     /locus_tag="PLG14_07430"
     CDS             1280..1414
                     /locus_tag="PLG14_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49005.1"
                     /translation="MNAKTARGSPASGTLAAALASERVRRGPHRWAWRGAAPSPASRA
                     "
     gene            1554..2603
                     /gene="trpS"
                     /locus_tag="PLG14_07435"
     CDS             1554..2603
                     /gene="trpS"
                     /locus_tag="PLG14_07435"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOX49006.1"
                     /translation="MDRKRILTGDRPTGKLHIGHYVGSLKNRVRLQDEYECFFIIADL
                     HTLTTKPEKENIESLADNARSAALDYLACGIDPQKSVIYLQSAVPEVIELSLFFQNLV
                     TVPRLSRIPSLKEMAQGAGIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNVAHVEI
                     TREIARRFNYLYGETFPVPEVLVSEHGSLVGLDGDAKMSKSLNNAIFLSDDEKTVVKR
                     VMSMYTDPKRVSADVPGTVEGNPVFQYHDAFNPDKAEVEDLKARYRAGKVGDVEVKEK
                     LAKALNAFLDPIRERRAKYESQPGLADEVVYDGTMRMREEARATLSLAKKAMGISAVW
                     NRISRKAEEVKKRRG"
     gene            2783..3280
                     /locus_tag="PLG14_07440"
     CDS             2783..3280
                     /locus_tag="PLG14_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOX49007.1"
                     /translation="MLDSAILAAVKAGMKGELDSITVYEDAAKAAEGEVREFFQERAE
                     EEKKHFNYLLGYYKKETINLTPERDAAAELGGKWKSAIVSEAFLRQVAVTKRLSAAVA
                     AAIHLESDAIRLYRDWAARAEAPALKKLLGTLVEWEEKHYADLLLIQGESERYYFDLN
                     RFEPF"
     gene            3388..4152
                     /locus_tag="PLG14_07445"
     CDS             3388..4152
                     /locus_tag="PLG14_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOX49008.1"
                     /translation="MSNTPGPLAFGLLVLAAAVAGFVDSIAGGGGIITLPALLAAGLP
                     PHLALGTNKLQSSFGSLAATLRYRAAGAIRIRDILPGLAATAAGAALGAVAVGAIDPA
                     FLRVLIPILLACILAFLALRPRFGASAGKRLVAPLPFWLGAGLVLGFYDGFFGPGVGT
                     FWAIALVSLAGLDLGAATANTKAANFASNVVSLGVFLAAGTVVAPLGLAMGAAQAVGA
                     LVGSRMVLKNGAPFVRVVLMVMTAGMIAYLVARYWL"
     gene            complement(4149..4784)
                     /locus_tag="PLG14_07450"
     CDS             complement(4149..4784)
                     /locus_tag="PLG14_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOX49009.1"
                     /translation="MPVVPLGPARAELVVVNSRFIASLSPASSAEEARAFIAAVRAEF
                     PDARHHVPAFVIGGGKSCVEYCSDDGEPSGTSGRPLLAVLKGSGLGDVAVVVTRYFGG
                     TLLGTGGLVKAYSEAGRRVLALVRRAETVETRRLEVPLPYHLYERFRILARESGAAIA
                     DERFEEIVRIVLEVPEDAFASFSGGLAELSAGSVPARELSRGSMRRPLDQA"
     gene            complement(4845..6179)
                     /locus_tag="PLG14_07455"
     CDS             complement(4845..6179)
                     /locus_tag="PLG14_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOX49010.1"
                     /translation="MKILVGHSNMDLDCVASIVLARYLFPDHVPVRSSLVHPSARKLL
                     NLYEDRLGFAAPGDLKGERIERMVVVDARSLDRISEHLKGADPEAMEIEVFDHHPPAG
                     RDIPGALVHERALGANASHLALILKERGAKVSPEDATVALVGIYSDTGNFTHENAREE
                     DFEAAAWLLAAGASLKLVKDLIEPLKERRQLVLFHEVLNRLERRTVRGYLVQTCYFEL
                     EEDALGLGAVIERVFEIEDGEILFGFFYFPSKGKMLVIARNSVEGVRLDEILADLGGG
                     GHAKAASATVKTQSGPELAEMILAYLEAALVPAATARDIMSEDVFTIDAEVSLLAASM
                     FLERVNHTGVPVVGEGGKLVGFLTLRDIMTGRRAGQMHVPLKTFMSRKLVTAPPGTTV
                     REIDDILFENNIGHLPIVEEGRLVGIVTRSDFLAFKRSDRRRRGALLAGLGA"
     gene            6440..6964
                     /locus_tag="PLG14_07460"
     CDS             6440..6964
                     /locus_tag="PLG14_07460"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOX49011.1"
                     /translation="MNDYAEIPLAEAYELQNAGGLVLVCTRGPDGRYDLAPVAWCCPL
                     DNGPASRLILVLDTGHRTYEDLSEAGEFAVALPAKGQAELVRRAGSVSGHDVDKYGKL
                     GIAAFPAEKVDALVPEGVAGWLECRLLRIVVEGSSAVVMGEVLRAAARPEAWKERLHF
                     VREGLMFSPRDRIE"
     gene            6970..>7537
                     /locus_tag="PLG14_07465"
     CDS             6970..>7537
                     /locus_tag="PLG14_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49012.1"
                     /translation="MEHLILGFYVLFFSTGFMGAAALVFLRIRSGERLLGLFLAFQLL
                     FVAGLALVGAYFYLGNVVLAGSRGESGMGPSAELAFGLVSSLVNGSIYLLAIAIARRL
                     EPEGGRRGLSLTAQGLAALVVLETAATVGLAVAEASGSAAAARVLSSPEWHLGGYLLT
                     GLAIAAFGFVATGAAAREERASIKALLRG"
BASE COUNT         1102 a         2774 c         2623 g         1038 t
ORIGIN      
        1 acgtcgtcga agcgcctgat cgcgatccgc tccgtctcga tgacgcagag ggccctgccc
       61 tcccagtcga cgatgacgga gaggtccccc gccgcggggg cgggctcgcc gccgcgctcc
      121 agggcgcgca aggaggtggc cgtgccgcgc ttccgccccg ccagggccag agcgccgagc
      181 tcgtccgcgt tcgccttgtc ggcgcagaaa tgccaggccg tatggccgcg ccgctccgcc
      241 ctcgcgcgcg cctccccgga aagggagccc aggtaggccg cccacatcgc ggcgacggag
      301 ggatcctccg gcttgcccgc cgcggcctgc gaggccgcct gcgaggcggc ggccgcgcct
      361 cccgagccgg cgtgcgcggc gtcaggactc gcgatgccgg aaacgggccc ggcggccgcg
      421 tccagcatga agtccacggc cgccagggcg tggaggcgcg tcgtgtcgaa gtagggaacg
      481 cccaggtcgc cgtcgtccat cgcgaggggg agctccgtgc agcctaggac gatcccctcg
      541 gcgccgcgct cctcgaggcc gcgggcgacc tcgcggagcc gcgcgcggga gggggcggag
      601 aagacgccgc ggcagagctc gtcgaagatg accgcgtcca cctcgcggcg ctccgcctcg
      661 ggcgggacga gcgtctcgat cccgaagcgc tcgcgcaggc gtccggagta gaagtcgcct
      721 tccatcgtat aacgcgtccc aaggaggccg acccgggccg ccccccgcgc gcgggccgcc
      781 gcgcccgccg cgtcggcgat gtggaggagg ggcaggcccg tcgccgcctc gagctccggc
      841 gcgaagcggt gcatcgtgtt cgtcgcgacg aggacggcct ccgcgccggc ccgcttcagg
      901 gccagagcct cgcccaccag ggccagacgg atctcgtccc agcgcccctc gcgcatgcgc
      961 gaggcgtagc ccgagaagtc caccgaggcc aggtagagcc tggccgagga gaggccgccg
     1021 gcgcggcgcg ctacctcctc gttgaggata cggtagtatt ccgccgtcga ttcccagctc
     1081 atcccgccca gtatgccgat cgcgcgcaag gccagcctcc cgtcggccgc cttgagggcg
     1141 gccagacgac actttcgccc gggaggccgc cgcggtcaag cgccgcgcgg agagaggcgg
     1201 ccggcgcgcg gagcgacgag gcccgcggcc ttcccccgct tgcggaagga gccggcgttt
     1261 ctatatgctg tgaggcgcca tgaacgccaa gaccgcccgc ggctcccccg cctccggaac
     1321 cctcgccgcc gcgctcgcct ccgagcgcgt ccgccgcggc ccccatcgct gggcgtggcg
     1381 cggggcggcg ccgtctcccg cctcccgcgc ctagggcgcg ggcccggggc ggaacccccg
     1441 ggcccgaggg gcccgaggcc ggcggcgccg cccatccttc cgcgagcagt agcccatacg
     1501 acccgagcgc cgcgcgcctt ccgcgcggcg acaccaagca tacggagaag aacatggaca
     1561 ggaagcgcat cctcacgggc gaccgcccta ccggcaagct ccacatcggc cactacgtcg
     1621 gctccctcaa gaaccgcgtc cgcctccagg acgagtacga gtgcttcttc atcatcgccg
     1681 acctgcacac cctcacgacg aagcccgaga aggagaacat cgagtccctg gcggacaacg
     1741 cgcgctcggc ggccctcgac tacctcgcct gcggcatcga cccccaaaag agcgtcatct
     1801 acctccagtc cgccgtgccc gaggtgatcg agctctccct cttcttccag aacctcgtca
     1861 ccgtgccgcg cctctcgcgc atccccagcc tcaaggagat ggcccagggc gcgggcatcg
     1921 aggagatgcc cttcggcctc ctcggctacc ccgtcctcca ggccgccgac atcctcctcc
     1981 cccgcgccca cctcgtgccc gtgggcaagg acaacgtcgc ccacgtggag atcacccgcg
     2041 agatcgcgcg ccgcttcaac tacctctacg gcgagacctt ccccgtgccc gaggtcctgg
     2101 tctccgagca cggctcgctc gtgggcctgg acggcgacgc caagatgtcg aagagcctca
     2161 acaacgcgat cttcctctcc gacgacgaga agaccgtcgt gaagcgcgtc atgtccatgt
     2221 acaccgaccc caagcgcgtc tccgccgacg tccccggcac cgtggagggc aaccccgtct
     2281 tccagtacca cgacgccttc aatccggaca aggccgaggt cgaggacctg aaggcccgct
     2341 accgggccgg caaggtgggc gacgtcgagg tgaaagagaa gctcgcgaag gccctcaacg
     2401 ccttcctgga ccccatccgc gagcgccgcg ccaagtacga gtcccagccg ggcctggccg
     2461 acgaggtcgt ctacgacggc acgatgcgca tgcgcgagga ggcccgcgcc accttgagcc
     2521 tcgcgaagaa ggcgatgggc atctccgccg tctggaaccg catctcccgc aaagccgaag
     2581 aagtgaagaa aaggcgcggc tagcgccttt tcttaagcgg atttagcgaa gcgattggaa
     2641 gtgaagaaaa ggcgcggcta gcgccttttc tcaagcggac ttggcggagc gattggaagt
     2701 gaagaaaagg cgcggccacc gccttgccct tgcgcgcgcc tgacttgcgg ctatagttga
     2761 agcgagtcca aggaggcaaa ccatgctcga tagcgccatc ctggccgccg tcaaggcagg
     2821 catgaagggc gagctcgact cgatcaccgt ctacgaggac gccgccaagg cggccgaggg
     2881 cgaggtcagg gagttcttcc aggagagggc cgaggaggag aagaagcact tcaactacct
     2941 cttaggctac tacaagaagg agaccatcaa cctgacgccg gagcgcgacg ccgccgccga
     3001 gctcgggggg aagtggaagt cggccatcgt gagcgaggcc ttcctcaggc aggtcgccgt
     3061 gaccaagcgg ctctccgcgg ccgtcgcggc ggccatacac ctcgaatccg acgccatccg
     3121 cctctaccgc gactgggccg ccagggcgga ggcgccggcg ctcaagaagc tcctcgggac
     3181 cctcgtcgag tgggaggaga aacactacgc cgacctcctc ctcatccagg gcgagtcgga
     3241 gcgctactac tttgacctga accgcttcga gcccttctag ccaggggcgc agaggcggac
     3301 cgccggccgg gccttcgccc gcggccggcg gactagaggg gcggcggggc ctgggcggcc
     3361 ccgccgcccc tcggctatga tgcgggcatg agcaacacac cgggccccct ggccttcgga
     3421 ctcctcgtcc tcgcggcggc cgtcgcgggc ttcgtcgaca gcatcgcggg cggcggcggc
     3481 atcatcaccc tcccggccct cctggccgcg ggacttcctc cccacctggc cctgggaacg
     3541 aacaagctcc agtcgagctt cgggagcctc gcggcgacgc tccgctaccg cgcggccgga
     3601 gccatcagga tccgggacat cctgccgggc ctcgcggcca cggccgccgg cgccgccctg
     3661 ggcgcggtcg cggtcggggc catcgacccc gccttcctcc gggtgctgat ccccatcctc
     3721 ctcgcctgca tcctcgcctt cctggccctg cgcccccgct tcggcgcctc agcgggcaag
     3781 cgcctcgtcg cccccctgcc cttctggctc ggcgccggcc tggtcctggg cttctacgac
     3841 ggcttcttcg ggccgggcgt cggcaccttc tgggccatag ccctcgtgag cctggccggc
     3901 ctggacctgg gggcggcgac ggccaacacg aaggcggcca acttcgcgag caacgtcgtg
     3961 tcgctcggcg tcttcctcgc ggcgggcacg gtggtcgctc ccctgggcct ggccatgggc
     4021 gcggcccagg ccgtcggcgc cctggtcggg agccggatgg tgctcaagaa cggcgccccc
     4081 ttcgtgcggg tcgtcctcat ggtgatgaca gcggggatga tcgcctacct cgtggcccgc
     4141 tactggctct aggcctggtc gagcggcctg cgcatgctgc cacgcgagag ttcgcgggcc
     4201 gggacggagc cggcggagag ctctgcgagc cccccagaga aggaggcgaa ggcgtcctcc
     4261 ggcacctcga ggacgatccg cacgatctcc tcgaagcgct cgtcggcgat cgcggccccg
     4321 gactcgcgag ccaggatcct gaagcgctcg tagagatggt aggggagcgg cacttcgagg
     4381 cgccgcgtct cgacagtctc cgcgcggcgg acgagggcca ggacgcgccg gccggcctcg
     4441 gagtaggcct tcacgaggcc gcccgtgccg aggagggtcc cgccgaagta cctcgtcacg
     4501 acgacggcga cgtctcccag gccggagccc ttgagcacgg ccaggagcgg cctccccgag
     4561 gtccccgaag gctcgccgtc gtcggagcag tactccacgc aggacttccc tcccccgatg
     4621 acgaaggccg gcacgtggtg ccgggcgtcg gggaattccg ccctgacggc ggcgatgaag
     4681 gccctcgcct cctccgccga ggaagcgggc gagaggctcg cgatgaagcg ggagttgacg
     4741 acgacaagct cggcccgcgc gggcccgagg gggacgacgg gcatgggagg actatagcac
     4801 gccgcgcggc ggggagtccg cccgcaaagg ccctgcccgg ccccttaggc gccgagcccc
     4861 gcgaggaggg cgccgcggcg cctgcggtcg ctgcgcttga aggcgaggaa gtccgacctc
     4921 gtcacgatgc cgaccaggcg cccctcctcg acgatcggaa ggtggccgat gttgttctcg
     4981 aagaggatgt cgtcgatctc ccgtaccgta gtgccgggcg gcgcggtcac gagcttgcgg
     5041 gacatgaagg tcttgagggg cacgtgcatc tggcctgccc ggcggcccgt catgatgtcg
     5101 cgcagggtga ggaagccgac gagcttcccg ccctcgccga cgacgggcac gcccgtgtgg
     5161 ttgacccgct ccaggaacat ggaggcggcg aggaggctca cctcggcgtc gatggtgaag
     5221 acgtcctccg acatgatgtc gcgcgcggtc gcggcgggca ccagggccgc ctccaggtag
     5281 gcgaggatca tctccgcgag ctccgggccc gactgggtct tcacggtggc ggaggcggcc
     5341 ttcgcgtggc cgccgccgcc caggtcggcc aggatctcgt ccaggcggac cccttccacc
     5401 gagttgcgcg cgatgaccag catcttgccc ttggacggga agtagaagaa gccgaagagg
     5461 atctcgccgt cctcgatctc gaagacgcgc tcgatcaccg cgcccaggcc cagggcgtcc
     5521 tcctcgagct cgaagtagca ggtctgcacc aggtagcccc ggacggtgcg gcgctccagg
     5581 cggttcagca cctcgtggaa gaggacgagc tggcgcctct ccttgagcgg ctcgatgagg
     5641 tccttgacga gcttgaggga ggcgcccgcc gcgaggagcc aggccgcggc ctcgaagtcc
     5701 tcctcgcgcg cgttctcgtg ggtgaagttg ccggtgtcgg agtagatgcc gacgagggcg
     5761 acggtcgcgt cctcggggct gaccttcgcg ccccgctcct tgaggatgag ggcgaggtgg
     5821 ctcgcgttgg cgcccagggc ccgctcgtgg acgagggcgc cgggtatgtc gcgcccggcg
     5881 gggggatggt ggtcgaagac ctcgatctcc atcgcctcgg gatccgcgcc cttgaggtgc
     5941 tcggagatcc ggtccaggct ccgcgcgtcg acgacgacca tacgctcgat ccgctcgccc
     6001 ttgaggtcgc cgggcgcggc gaagccgagc ctgtcctcgt agaggttgag gagcttccgc
     6061 gccgaggggt ggacgaggga gctgcgcacc gggacgtggt ccgggaagag gtagcgggcg
     6121 aggacgatgg acgccacgca atcgaggtcc atgttcgagt gtccgacgag tatcttcatc
     6181 gctcggcgaa gtatagccgc ggccgccggg aaagcaaggc cggcgccgct cgcttggccg
     6241 gcccgcggcc cggcggcacg gcggcgcgca atcgccgcgg cccgtcggca cggcggcgcg
     6301 gcggcgggcc gcgacccggg cggcgcggct cctcgcgcgg gctgaggcgg cgccgcccca
     6361 agcgccgccg cgctcctcgc cgagcctgta cctgcgcccc ggaatgcgat accatctcaa
     6421 gccgagaacg gaggatgcca tgaacgacta cgccgagatc cccctcgccg aggcctacga
     6481 gctccagaac gccggcggcc tcgtcctcgt ctgcacccgc ggccccgacg gacgctacga
     6541 cctcgcgccc gtcgcctggt gctgccccct cgacaacggc ccggcctccc ggctgatcct
     6601 cgtcctcgac acgggacacc ggacctacga ggacctctcc gaggcgggcg agttcgccgt
     6661 cgccctcccg gccaagggcc aggccgagct cgtgagacgt gcgggctcgg tctcgggcca
     6721 cgacgtcgac aagtacggga agctcggcat cgcggccttc cccgcggaaa aggtggacgc
     6781 cctcgtcccc gagggcgtgg cgggatggct cgagtgccga ctcctccgca tcgtcgtgga
     6841 ggggagctcg gcggtggtca tgggcgaggt cctgcgcgcc gcggccaggc ccgaggcctg
     6901 gaaggagcgc ctccacttcg tccgcgaggg cctcatgttc tcgccgcggg acaggatcga
     6961 gtagccgcca tggagcacct catcctcggc ttctacgtcc tcttcttctc cacgggcttc
     7021 atgggggcgg cggccctcgt cttcctcagg atccgctcgg gcgagcgcct cctcggcctc
     7081 ttcctcgcct tccagctcct cttcgtggcg gggctcgccc tcgtcggcgc ctacttctac
     7141 ctgggcaacg tcgtcctcgc ggggagccga ggggagagcg gcatgggccc ctcggcggag
     7201 ctcgccttcg gcctcgtctc ctcgctcgtg aacggctcga tctacctcct cgcgatcgcg
     7261 atcgcgcggc ggctcgagcc cgagggcggc cgccggggac tgagcctcac cgcccagggc
     7321 ctcgccgccc tcgtcgtcct cgagaccgcg gccaccgtcg gcctcgcggt cgcggaggcc
     7381 tcgggctccg cggccgcggc ccgcgtgctc tcgtcgcccg agtggcacct gggcggctac
     7441 ctcctcaccg gcctcgcgat agccgccttc ggcttcgtcg cgacgggggc cgcggccagg
     7501 gaggagcggg cctccatcaa ggccctcctg cggggct
//
LOCUS       DAOOSJ010000129         7441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6206_length_7441_cov_5.943000, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000129 DAOOSJ010000000
VERSION     DAOOSJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6206_length_7441_cov_5.943000"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..359)
                     /locus_tag="PLG14_07470"
     CDS             complement(9..359)
                     /locus_tag="PLG14_07470"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOX49013.1"
                     /translation="MPSFNGLGPNGAGSMSGRRLGPCGGGQAARRGFGYGAGPRGAGR
                     GFGRGAGYGRGWNLGPGLGRFAVGYGPEDSDTRAEGLRQALEARVSLLRAELARAETL
                     LMESQAERAGEEER"
     gene            complement(534..1229)
                     /locus_tag="PLG14_07475"
     CDS             complement(534..1229)
                     /locus_tag="PLG14_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOX49014.1"
                     /translation="MGAFDEPRGGALPRGPLAHEDRKALPPEARAFLEAMERRYACKL
                     YDGKPLPRALEDYILECGRLSPSSFGLEHWRFVAVREAAAKERLFAACFAQESVRTAS
                     LVVVALARRAAAYGPDTDFVRARAERFPGGWPVFREDYKGYYAFLAENGLLEHWAKAQ
                     TYIACANMMTGAAAAGLDSCAIEGYKEPEVLSAIGADPGEWLVGIVTVFGRRAEEPRE
                     KIREPFEAVVDYR"
     gene            complement(1229..1972)
                     /gene="deoD"
                     /locus_tag="PLG14_07480"
     CDS             complement(1229..1972)
                     /gene="deoD"
                     /locus_tag="PLG14_07480"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOX49015.1"
                     /translation="MHDKSLDHYKDGTPHNTAKSGDIARTVLMPGDPLRAKHLAENFL
                     EGAKQFNAVRNMFGYTGTYKGKPVSVMGSGMGGPSIGLYSYELFAFYGVERVIRIGTC
                     GGLTSAVEVGDLVLAMASSGDSNYAHQYRLNGSFSPCADFELLEKAVGAARSRKIPYW
                     VGNVFCSDVFSLYSALPEGEGWAKWAKMGCAATDMESFALYCNAAYLGKKALAMFTCS
                     DSNVSRKEMSPAERQTALTSMFEVALEIA"
     gene            complement(2079..2981)
                     /locus_tag="PLG14_07485"
     CDS             complement(2079..2981)
                     /locus_tag="PLG14_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49016.1"
                     /translation="MSSLLGMLPSVLMIVAPILIAALGGMISERSGVVNIALEGLMGI
                     GAFAAATTHVLLESSMRGSVWLALLVATAAGMAFSLIHAYASVSLKADQTVSGTGINL
                     LSMGGTVFLCQIIFRQERTEPYKLGMMPGLGGIYPTAYIALAVLLATWYVLYKRPFGL
                     RLRACGEHPAAAASAGIDVKKMRYIAVLASGAYAGLAGGCLVLTQTIQFTNMTINGAG
                     FIALAAVSFGRYLPKGIAGSSVLFGSAVALAIYMVNIEALRGLPPEFFSIMPYLITLV
                     TLVAFSGKDYAPRASGQPYEKGGS"
     gene            complement(2974..4116)
                     /locus_tag="PLG14_07490"
     CDS             complement(2974..4116)
                     /locus_tag="PLG14_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49017.1"
                     /translation="MAFASKDSAKAKARADLFDAFIGSNGAISVIVVVLGFLCGTLLI
                     LAVGKNPGNMYAALLQTLTGLDVTRGTWNARYIGEWLTASMPLILCGLSMGFAARTGL
                     FNVGAEGQYIVGLTAAQFVALFGPQVPGLHWALALVAGCLAGALWGGLVGWLKAKYSV
                     SEVVATIMLNYVAYYLSRFFTQLMPAANTYRTPAYPETALLKNGLFERLTNGSRLNTG
                     LFVVVICVVFYWFVIERTRLGFELRATGLNKEAARCSGIKVTRSITASMAIAGAFAGL
                     AGVIVALGSFSYGRVLGGLDNYGFNGIAVALVGNSTAGGTLLSGLLFGMLASAQPLMQ
                     SRQIPKEVTWIISGLVVVFISLRAGFKMIMDWRQKRLAEKGRQRDE"
     gene            complement(4118..5689)
                     /locus_tag="PLG14_07495"
     CDS             complement(4118..5689)
                     /locus_tag="PLG14_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49018.1"
                     /translation="MDEYAIEMLDITKVFPGVVANDKVTLRVRENEIHAILGENGAGK
                     STLMSVLFGLYDPDGGSIRVKGKEVRIRDPNEATALGIGMVHQHFKLVHTYTVTENII
                     LGMEPRRRDGTIDIASAERRVAELSERYGLSVDPRAKIESITVGMQQRVEILKILYRD
                     ADILIFDEPTAVLTPQEIDELMSIFERLKAEGKTIILITHKLREIKEAAERCTVLRRG
                     RFIGTVDVASTTEEEMAEMMVGRAVKFRIDKDEAKPGEVVLKVEDLTVMSSKGHPAVK
                     GLSLEVRAGEIVGLAGVDGNGQSELIQAITGLKPAKSGRILLEGRDIAHRSIKERVEA
                     GIGHVPEDRHKHGLVLDFRLDENLALKTYDKKPFLKRLGVLDFAAMAAHGDDLIGQYD
                     IRAGKGSATKARNMSGGNQQKAIIAREIDLSPRLLVVAQPTRGLDVGSIEYIHKRVVA
                     ERDKGRAVLLVSFELDEIMALCDRIAAISKGEIVGVVDAATAEERAIGAMMAGVRGAH
                     PAAGRRDERPAQGAS"
     gene            complement(5810..6919)
                     /locus_tag="PLG14_07500"
     CDS             complement(5810..6919)
                     /locus_tag="PLG14_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX49019.1"
                     /translation="MKRSVVTILALGLALLAISCGGKAAKPADTAGLPTARLLTDATG
                     IDDKSFNAAAWRGILDFYGDTWENQAKKGKSYDVVTAQTQDMYVPNLKQASDEKYDLV
                     VVTGFTWADALGEVAKQYPDQKYMIVDVDWVAGSNVMQMTFSEHEGSYLVGVAAALKA
                     KADGIKDAKFGFIGGIPGPVITKFEMGWVQGIKSVLPDAKIVDYYANDWGKPELAKAQ
                     AKNWYDSGVYAIFSAAGGTGNGTIAQAKEYRSQGKNVWAVGVDSDQFADGVYAEGKSA
                     VLTSMLKRVETASKYALDAVKNKTFKAEVVVLDTKAEGVGFSDKNPELGADIVAKVNE
                     AKAKIVAGDIKVAATYADAKALPGFPQNLQASDKQ"
     gene            complement(7083..>7441)
                     /locus_tag="PLG14_07505"
     CDS             complement(7083..>7441)
                     /locus_tag="PLG14_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49020.1"
                     /translation="SGDEALKPVRVLSGGEKVRCVLSKLMLSGANCLILDDPTNHLDL
                     EAITALNDGLIEFPGVLLFTSHDHEFITSIANRMVEICPGGVIDRYMSFDEYIKDKQV
                     AELRDQYYHGHTIAEL"
BASE COUNT         1141 a         2308 c         2740 g         1252 t
ORIGIN      
        1 ggcttcgctc accgctcttc ttctccagcc cgttccgcct ggctctccat gagcagggtt
       61 tcggcccgcg cgagctcggc gcggaggagg gagacgcgcg cctcgagggc ttgcctcagc
      121 ccttcggcgc gcgtgtcgga gtcctccggg ccgtagccga cggcgaagcg gccgagcccg
      181 gggccgaggt tccagcctcg accgtagccg gcgccgcgtc cgaagcctcg gcccgccccg
      241 cggggccccg cgccgtaccc gaaaccgcgg cgagcggcct ggcctccgcc gcaggggccg
      301 agcctccttc cgctcatcga tccagcccca ttggggccca gtccattgaa actaggcatg
      361 acatgcctcc ttgcgttata ggacatatac ttgcgccgac ggcgcatggg ataaaataag
      421 catatgctta attcaacctg aggtaaatat gagcaaaagc ccgctatctg tcaagtggca
      481 gcgcgaaaaa aaacccgcga cgtctgtcgc gggctctggc atcaagcgag cttctagcgg
      541 tagtcgacga cagcctcgaa aggctcgcgg atcttctccc gcggctcctc ggccctgcgg
      601 ccgaagacgg tgacgatgcc gacgagccat tccccgggat cggcgccgat cgcggagagg
      661 acctccggct ccttgtagcc ctcgatcgcg caggagtcga ggccggcggc cgccgcgccc
      721 gtcatcatgt tcgcgcaggc gatgtaggtc tgggccttgg cccaatgctc aaggagcccg
      781 ttctccgcga ggaaggcgta gtagcccttg tagtcctcgc ggaagaccgg ccagcctccc
      841 gggaagcgct cggcgcgggc cctcacgaag tccgtgtcgg ggccgtaggc ggcggcgcgc
      901 cgcgcgaggg cgacgacgac gagggaggcc gtgcggacgc tctcctgggc gaagcaggcc
      961 gcgaagagcc gctccttggc cgcggcctcg cggacggcga cgaagcgcca gtgctcgagg
     1021 ccgaaggagg agggcgagag gcgcccgcac tcgaggatgt agtcctcgag cgcgcggggc
     1081 agcggcttcc cgtcgtagag cttgcaggcg tatctccgct ccatcgcctc caggaaggcc
     1141 cgcgcctccg gaggcagggc cttcctgtcc tcgtgggcta ggggtccgcg ggggagagct
     1201 ccgccgcggg gctcgtcgaa ggcgcccatc aagctatctc gagggccacc tcgaacatcg
     1261 aggtgagggc ggtctgcctc tcggcggggc tcatctcctt gcggctcacg ttggagtccg
     1321 agcaggtgaa catcgccagg gccttctttc cgaggtaggc ggcgttgcag tagagggcga
     1381 agctctccat gtccgtcgcg gcgcagccca tcttggccca cttggcccag ccctcgccct
     1441 cggggagggc gctgtagagg gagaagacgt cggagcagaa gacgttgccg acccaatagg
     1501 ggatcttcct cgacctggcc gcgccgaccg ccttctccag gagctcgaag tcggcgcagg
     1561 ggctgaagct tccgttgagc cggtactggt gggcgtagtt cgagtcgccg gaggaagcca
     1621 tcgcgaggac gaggtcgccc acctcgacgg cggaggtgag gcccccgcag gtgccgatgc
     1681 ggatcacgcg ctcgacgccg tagaaggcga agagctcgta ggagtagagg cctatggagg
     1741 ggccgcccat gcccgagccc atgacggaga cgggcttgcc cttgtaggtg cccgtgtagc
     1801 cgaacatgtt cctgacggcg ttgaactgct tcgcgccctc caggaagttc tccgcgaggt
     1861 gcttggcgcg aagggggtcg ccgggcatga ggactgtcct ggcgatgtcg ccggacttgg
     1921 ccgtgttgtg cggcgtcccg tccttgtagt ggtctaggct cttgtcgtgc atgtggtctc
     1981 ctggagggcc cccggggcgg ccgggccggc cggcgaccct ggggattcga tggaaagcgc
     2041 ggcggctgcg cggagctccg cgcagccgcc ttgaggcttc agctcccgcc cttctcgtag
     2101 ggctggccgg acgcgcgggg cgcgtagtcc ttgcccgaga aggcgacgag ggtcacgagg
     2161 gtgatgaggt agggcatgat gctgaagaac tccggcggca ggccgcggag cgcctcgatg
     2221 ttgaccatgt agatcgcgag cgcgacggcg gagccgaaga ggacgctcga gcccgcgatg
     2281 cccttgggca ggtagcggcc gaagctgacc gcggccaagg cgatgaagcc cgcgccgttg
     2341 atggtcatgt tcgtgaactg gatggtctgg gtgaggacga ggcagcctcc cgcgaggccc
     2401 gcgtaggcgc ctgaggcgag gacggcgatg tagcgcatct tcttgacgtc gatgccggcg
     2461 gaggcggccg cggccgggtg ctcgccgcag gcgcggaggc gcaggccgaa gggccgcttg
     2521 tagaggacgt accaggtggc caggaggacg gcgagggcga tgtaggccgt cgggtagatc
     2581 ccccccaggc ccggcatcat cccgagcttg tagggctccg tcctctcttg cctgaagatg
     2641 atctggcaga ggaagaccgt gccgcccatg gagaggaggt tgatgcccgt gccggagacc
     2701 gtctggtctg ccttgaggct cacggaggcg taggcgtgga tgagggagaa ggccatgccg
     2761 gccgcggtgg ccaccaggag ggcgagccag accgagcccc gcatcgagga ctcgaggagg
     2821 acgtgggtcg tcgccgccgc gaaggcgccg atgcccatga ggccctccag ggcgatgttg
     2881 acgacgcccg agcgctcgga gatcatgccg cccagggcgg cgatgaggat gggcgcgacg
     2941 atcatgagga cggaggggag cattccgagc aggctactca tcgcgctgcc ttcctttctc
     3001 ggccaggcgc ttctggcgcc aatccatgat catcttgaag cccgcccgga gcgagatgaa
     3061 gacgacgacc aggccggaga tgatccacgt tacctccttg gggatctggc gggactgcat
     3121 gaggggctgg gcggaggcga gcatgccgaa gaggaggccg gagaggaggg ttccgcccgc
     3181 ggtgctgttg cccacgaggg ccaccgcgat gccgttgaag ccgtagttgt ccaggcctcc
     3241 caggacccgg ccgtagctga aggagcccag ggcgacgatg acgccggcca ggcccgcgaa
     3301 ggcgcccgcg atcgccatgg aggcggttat gctccgcgtc accttgatgc cggagcagcg
     3361 cgcggcctcc ttgttgaggc ccgtggcgcg gagctcgaag ccgagccgcg tgcgctcgat
     3421 gacgaaccag tagaagacga cgcagatgac cacgacgaag agccccgtgt tgagcctgga
     3481 gccgttcgtg agcctctcga agaggccgtt cttgaggagg gccgtctccg ggtaggcggg
     3541 ggtcctgtag gtgttcgccg cgggcatgag ctgggtgaag aagcgcgaga ggtagtaggc
     3601 gacgtagttg agcatgatcg tcgcgacgac ctcggagacg gagtacttgg ccttgagcca
     3661 gccgaccagg cctccccaga gggcgccggc caggcagccc gcgaccaggg ccagggccca
     3721 gtgcaggccg gggacctggg gtccgaagag ggcgacgaac tgcgccgccg tgaggccgac
     3781 gatgtactgc ccctcggcgc cgacgttgaa gaggccggtg cgggcggcga agcccatgga
     3841 gaggccgcag aggatgaggg gcatggaggc cgtgagccac tcgcctatgt agcgggcgtt
     3901 ccaggtcccg cgggtcacgt ccaggccggt gagggtctgg aggagggccg cgtacatgtt
     3961 cccggggttc ttcccgacgg ccaggatgag gagggtgccg cagaggaagc cgaggacgac
     4021 gacgatgacg gagatcgccc cgttggagcc gatgaaggcg tcgaagaggt ccgcccgggc
     4081 cttcgccttg gcgctgtctt tgcttgcgaa ggccatgtca ggacgctcct tgcgcgggcc
     4141 gttcgtcgcg gcgtccggcg gcggggtggg cgccgcggac gccggccatc atggcgccga
     4201 ttgcgcgctc ctccgcggtg gcggcgtcga cgacgccgac gatctcgccc ttggagatgg
     4261 cggcgatgcg gtcgcagagg gccatgatct cgtcgagctc gaaggagacg aggaggacgg
     4321 cccgcccctt gtcccgctcg gcgacgacgc gcttgtggat gtactcgatg gagcccacgt
     4381 ccaggccccg ggtgggctgg gcgacgacga ggaggcgggg ggagaggtcg atctcccgag
     4441 cgatgatcgc cttctgctgg ttgccgcccg acatgttccg cgccttcgtg gccgagccct
     4501 tgcccgcgcg gatgtcgtac tgcccgatga ggtcgtcgcc gtgggcggcc atcgcggcga
     4561 agtccaggac gcccaggcgc ttcaggaagg gcttcttgtc gtaggtcttg agcgccaggt
     4621 tctcgtccag gcggaagtcc agcacgaggc cgtgcttgtg cctgtcctcg gggacgtggc
     4681 cgatgccggc ctcgacccgc tccttgatgg agcggtgggc gatgtcccgg ccctccagga
     4741 ggatgcggcc cgacttcgcg ggcttgaggc cggtgatggc ctggatgagc tcggactggc
     4801 cgttgccgtc gacgccggcc aggcccacga tctcgccggc cctgacctcc agggagaggc
     4861 ccttcacggc gggatggccc ttggagctca tcacggtgag gtcctcgacc ttgaggacga
     4921 cctcgcccgg cttcgcctcg tccttgtcga tgcggaactt gaccgcgcgg ccgaccatca
     4981 tctccgccat ctcctcctcg gtcgtcgagg ccacgtcgac ggtgccgatg aagcgtccgc
     5041 ggcgaaggac ggtgcagcgc tcggccgcct ccttgatctc gcggagcttg tgggtgatga
     5101 ggatgatcgt cttcccctcg gccttgaggc gctcgaagat ggacatgagc tcgtcgatct
     5161 cctgcggcgt gagcacggcc gtcggctcgt cgaagatgag gatgtcggcg tcgcggtaga
     5221 ggatcttgag gatctccacg cgctgctgca tgccgaccgt gatcgactcg atcttcgcgc
     5281 gcgggtcgac ggacagcccg tagcgctcgg agagctcggc cacgcgcctc tcggcgctcg
     5341 cgatgtcgat ggtgccgtcg cggcggcgcg gctccatgcc caggatgatg ttctcggtga
     5401 cggtgtaggt gtgcacgagc ttgaagtgct ggtgcaccat gccgatgccg agggccgtcg
     5461 cctcgttcgg gtccctgatc cggacttcct tgcccttcac gcggatcgag ccgccgtcgg
     5521 ggtcgtagag gccgaagagg accgacatga gggtggactt gcccgcgccg ttctcgccga
     5581 ggatggcgtg gatctcgttc tcgcgcacgc gaagggtcac cttgtcgttc gccacgacgc
     5641 cggggaagac cttcgtgatg tcgagcatct cgatggcgta ctcgtccatg tgggttccaa
     5701 tcgggaaaaa tgagaagacg gaggggcccc cggaccgcgg tcgcggagcc gggggccgcc
     5761 agccgtccgg ccggagcgag cctcaaggct cgtcccggcc tggggaagct tactgcttgt
     5821 cgctggcctg gaggttctgg gggaagccgg ggagggcctt cgcgtcggcg taggtcgcgg
     5881 cgaccttgat gtcgccggcg acgatcttgg ccttagcctc gttgaccttg gcgacgatgt
     5941 cggcgccgag ctcggggttc ttgtcggaga agccgacgcc ctccgccttg gtgtcgagga
     6001 cgacgacctc ggccttgaag gtcttgttct tcacggcgtc gagggcgtac ttggaggcgg
     6061 tctccacgcg cttgagcatg gaggtcagga cggcggactt gccctcggcg tagacgccgt
     6121 cggcgaactg gtcggagtcg acgccgaccg cccacacgtt cttgccctgg ctgcggtatt
     6181 ccttggcctg ggcgatggtg ccgttgccgg tgccgccagc cgcggagaag atcgcgtaca
     6241 cgccggagtc gtaccagttc ttggcctggg ccttcgcgag ctcgggcttg ccccagtcgt
     6301 tcgcgtagta gtcgacgatc ttggcgtcgg ggaggacgga cttgatgccc tggacccagc
     6361 ccatctcgaa cttggtgatg acggggccgg ggatgccgcc gatgaagccg aacttggcgt
     6421 ccttgatgcc gtcggccttg gccttgaggg cggccgcgac gccgacgagg taggagccct
     6481 cgtgctcgga gaaggtcatc tgcatgacgt tggagccggc gacccagtcg acgtcgacga
     6541 tcatgtactt ctggtcgggg tactgcttcg cgacctcgcc cagggcgtcg gcccaggtga
     6601 agccggtgac gaccacgagg tcgtacttct cgtccgaggc ctgcttgagg ttcgggacgt
     6661 acatgtcctg ggtctgcgcc gtgacgacgt cgtaggactt gcccttcttg gcctggttct
     6721 cccaggtgtc cccgtagaag tcgaggatgc cgcgccaggc ggcggcgttg aaggacttgt
     6781 catcgatgcc ggtggcgtcg gtgaggagcc gggcggtggg gagacccgcg gtgtcggcgg
     6841 gcttcgcggc cttaccgccg caggagatcg ccaggagggc gagcccgagg gcgaggatgg
     6901 tgacgacgga acgcttcatt cgttttcctc cgtacgtgtc tgggccgcgt caaccgcggc
     6961 cttttcgtac atcatacgac tgcccgccgg catggtcaag gcggtgcggg cctccggagg
     7021 cgcaaaaaaa gccgccccgc gggggacggc tttccttaaa acggacgact acgacgaagg
     7081 tatcagagtt cggctatcgt gtggccgtgg tagtactggt cgcggagctc ggctacctgc
     7141 ttgtccttga tgtactcgtc gaagctcatg tagcggtcga tgacgccgcc cgggcagatc
     7201 tccaccatgc ggttggcgat cgacgttatg aactcgtggt cgtggctcgt gaagaggagt
     7261 acgccgggga actcgatgag gccgtcgttg agggcggtga tcgcctccag gtccaggtgg
     7321 ttcgtcgggt cgtccaggat caggcagttc gcgccggaga gcatgagctt ggagaggacg
     7381 cagcgcacct tctcgccgcc ggagaggacg cggacgggct tcagggcctc gtcgccggag
     7441 a
//
LOCUS       DAOOSJ010000130         7380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6264_length_7380_cov_5.686416, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000130 DAOOSJ010000000
VERSION     DAOOSJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_7380_cov_5.686416"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLG14_07510"
     CDS             <1..856
                     /locus_tag="PLG14_07510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOX49021.1"
                     /translation="GEPYNQLLARIAMQAEVDIIILGGMGLKYDDYLYFKDALEEGGA
                     LQKTIMFVHTAADPVVECLQVPDISLAVAERFALKGKNVLVLLTDMTNFADAMKEIAI
                     TQEQVPSNRGYPGDLYSQLAARYEKAVDFSDAGSITILAVTTMPGDDVTHPVPDNTGY
                     ITEGQYYLKGGRIEPFGSLSRLKQQVNGKTRADHRAIMDGMIKLYASYKDSLEKKSMG
                     FQMSAWDKKLLKYGEAFEREMMDLSVNVPLEKALDKGWEILADCFEPRETALRTELIE
                     KFWPKRTA"
     gene            879..1511
                     /locus_tag="PLG14_07515"
     CDS             879..1511
                     /locus_tag="PLG14_07515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOX49022.1"
                     /translation="MAKIKLTKNELKKQKDALKMYKRYLPTLQLKKQQLQMEIRGIEA
                     RRRELEEAKAGLDRGFRAWIGVFGEAEAAYGADGRPLMTVTAVRTAEGNIAGVEIPVF
                     EGADFAFAEYDLFRVPLWVDSALERMKQVLLLDLEARVLDTQIERLAAELRTTTQRVN
                     LFEKVKIPETLDDIKRIRIYLGDQQTAQVVRGKIAKRKVVAAAPAAGSAS"
     gene            1520..3415
                     /locus_tag="PLG14_07520"
     CDS             1520..3415
                     /locus_tag="PLG14_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49023.1"
                     /translation="MKKVHLVVLEREKAQALSELGKLGVVHLEPLAGKGADFEKAGRS
                     LAEVERAVAILSSIKVPEPKKGGKAKEGKAPASAPAAVSDAGLREAGLAAAARCSALV
                     DEQKAAQDEGLALVRELERVQAWGDFEPGLAAELKAAGLGLRLYEGPLARLASLPEGL
                     EYLRLAAPRGRARIAVVERGGSPALPAEFAEFALPAESPAALREGVARAEARKAAAEA
                     GLASLAPSLPALQAAKAWLSEALLFEQARSGMGAEEGLAFLSGFAPERELPALKAAAA
                     SHGWALLSDDPAPEEAPPSKVENGPFVRMIAPVFDFLGTVPNYREYDISGLFLFFFCF
                     FFAMIFGDAGYGSILLAAGLFAALKARKAQGKVPDPIRLLLLLAASTVAWGVMTASWF
                     GMPPERLPAVLRAVDIYWISNENPASGDNVKMLCFLIGTVQLVVAHLKNIKRDIGSLK
                     FLAQIGQLSMVAGMLFLVLNLVISSERFPLPTWALYLVAGGFGLNFVFASYDGSKGLV
                     KGIVGGVVSSLANIVSVFLGVVNVFADIVSYIRLWAVGLAGIAISQTVNNMAGPMLGS
                     LIMFGAGVLLLVFGHGLNIMMSVLSVIVHGVRLNMLEFSGHLGMEWSGYKYEPFRATA
                     PEERLKG"
     gene            3431..3853
                     /locus_tag="PLG14_07525"
     CDS             3431..3853
                     /locus_tag="PLG14_07525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOX49024.1"
                     /translation="MNIGMIGVAAVLGLSAIGSAIGAGIAGQAAIGAWKRNYMANKPA
                     SFLLVVFAGAPLTQTIYGFILLQRMLASTKDPLLLLGAGIIGGAAMGMSAIAQGKASA
                     SSCDAFGETGKGFGQYITVVGLCETVALFVMAFTFGAI"
     gene            4123..5184
                     /gene="ispG"
                     /locus_tag="PLG14_07530"
                     /gene_synonym="gcpE"
     CDS             4123..5184
                     /gene="ispG"
                     /locus_tag="PLG14_07530"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOX49025.1"
                     /translation="MPSTRSVKAKGLIIGGGFPVSIQTMWKEPLRADGLEAAAARIAS
                     LAELGCDVLRFAVPDLDAAEVLGRLAAMTEMPLVADIHFDWRIALACLDHPIAKIRIN
                     PGNIGERWKVEEVARKAAGQGVPIRIGVNGGSLPQDLRARIDRGELDRAGALVEAAER
                     EVAAFEELGFAALVVSMKASSVEETLEVNRRFAKLRDYPLHLGVTEAGPLIPGIVRNT
                     AALVPLLKEGIGDTIRVSISDTMESEVAAGREILAAAGLGREGVTLVSCPRCARAGFD
                     THAFTAKWASRLYAMKKRATVAVMGCVVNGPGEARHADLGITGAGDKVVIFRKGKIAR
                     TISREEADTVFEEELRALE"
     gene            5181..6473
                     /locus_tag="PLG14_07535"
     CDS             5181..6473
                     /locus_tag="PLG14_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49026.1"
                     /translation="MKQHRPPMSQAFALAASALLCAAAPSAAAQSAAPRAAALSAAPQ
                     AAAASGPGGVSRAAAGADESWLRFQSGKRLFEERRFGEALNAFEDAAALRRDRFAWAQ
                     GRLDGVLSGEEARAAKDYLPALVDSLAKRDLIERDYAQVKKASGGSLRREAELLRQRR
                     LSDGFAGFLDALVLVLDHRPAESLGGSIAKLRSAAAELSSYPEAEYWIGRVYLAEGEL
                     RLAELQFRKAYDQRESLDIPEDRFQILDALAGVYRDGGKWRDYEESLKEIVAADPAFS
                     EKSRFFRDAMERSVRRDGIDKFMTLYRVEGGAWTKAGAELGSFYLANGRPQALVHLAL
                     AANGLLARCIERVREREPSYAYTSLRELLGRIDADRELRRYAEEAKLYRCLYELGEAL
                     AAEGERDSARGIWAALAGRDTAAPWDRRSAEALARAFR"
     gene            complement(6587..>7380)
                     /locus_tag="PLG14_07540"
     CDS             complement(6587..>7380)
                     /locus_tag="PLG14_07540"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOX49027.1"
                     /translation="RDGAILGLGGGVPCDLAAFAASLYMRGSRLVLVPTSLVAMADAA
                     LGGKAAVDLAGVRNLAGTFYAADEVRIHTPFLASLPEREYRSGLAEVLRTGLVAAPKV
                     RSLLSGRGADLRSRDLALLDETVRRCVLAKASLCERDPYDRGDRLRLNFGHTFAHALE
                     ALRPDILHGEAVAWGMARALDAGLELGLSDPEAAAEARSLIEAYGFETSYSGIEPEAL
                     LAAMRRDKKGLPGSLRLVLYKEECSVALVETPEAVALRALEKGLR"
BASE COUNT         1123 a         2701 c         2484 g         1072 t
ORIGIN      
        1 cggcgagccc tacaaccagc tcctggcccg catcgccatg caggccgagg tcgacatcat
       61 catcctgggc ggcatgggcc tcaagtacga cgactacctc tacttcaagg acgccctgga
      121 ggagggcggc gccctccaga agacgatcat gttcgtccac acggcggccg accccgtcgt
      181 cgagtgcctc caggtgcccg acatctccct ggccgtcgcg gagcgcttcg ccctcaaggg
      241 aaagaacgtc ctcgtcctcc tcaccgacat gacgaacttc gccgacgcga tgaaggagat
      301 cgccatcacc caggagcagg tcccctcgaa ccgcggctat cccggcgacc tctactccca
      361 gctcgcggcc cgctacgaga aggccgtcga cttctccgac gccggctcca tcaccatcct
      421 cgccgtcacg accatgcccg gcgacgacgt cacccacccg gtgccggaca acacgggcta
      481 catcaccgag ggccagtact acctgaaggg cggccgcatc gagcccttcg gctccctctc
      541 ccgcctcaag cagcaggtca acggcaagac ccgcgccgac caccgcgcca tcatggacgg
      601 catgatcaag ctctacgcct cctacaagga cagcctggag aagaagtcca tgggcttcca
      661 gatgtcggcc tgggacaaga agctcctcaa gtacggagag gccttcgagc gcgagatgat
      721 ggacctctcg gtcaacgtcc ccctggagaa ggccctggac aagggttggg agatcctggc
      781 cgactgcttc gagccacggg agacggccct gcggaccgag ctcatcgaga agttctggcc
      841 caagcggacc gcgtaaaagg ggcccgcgag gctaaacgat ggccaagatc aagctgacca
      901 agaacgagct caagaagcag aaggacgcgc tgaagatgta caagcgctac ctcccgacgc
      961 tccagctcaa gaaacagcag ctccagatgg agatccgcgg catcgaggcg aggcggcgcg
     1021 agctcgagga ggcgaaggcc gggctcgacc gaggcttccg cgcctggatc ggcgtcttcg
     1081 gcgaggccga ggccgcctac ggcgccgacg gcaggcccct catgacggtc accgccgtgc
     1141 gcaccgccga gggcaacatc gccggcgtcg agataccggt cttcgagggg gcggacttcg
     1201 ccttcgcgga gtacgacctc ttccgcgtgc cgctctgggt cgactcggcc ctggagcgga
     1261 tgaagcaggt cctcctcctc gacctggagg cccgcgtcct ggacacgcag atcgagcggc
     1321 tcgcggccga gctccgcacg acgacccaac gcgtcaacct cttcgagaag gtgaagatcc
     1381 ccgagaccct ggacgacatc aagcgcatcc ggatctacct tggggaccag cagacggccc
     1441 aggtcgtccg cggcaagata gccaagcgca aggtcgtcgc ggccgcgccg gccgccggga
     1501 gcgcctcatg atagcgccca tgaagaaggt ccaccttgtc gtcctggagc gggagaaggc
     1561 acaggccctc tccgagctcg ggaaactcgg cgtcgtgcac ctggagcccc tcgcgggcaa
     1621 gggcgccgat ttcgagaagg ccggccgctc cctggccgag gtggagcgcg ccgtcgccat
     1681 cctctcctcg atcaaggtcc ccgagcccaa gaagggcggc aaggcgaagg agggcaaggc
     1741 gccggcctcg gcccccgccg cggtgtcgga tgctggcctc cgcgaggcag gcctcgcggc
     1801 cgccgcgcgc tgctctgccc tcgtggacga gcagaaggcc gcccaggacg agggcctcgc
     1861 cctcgtccgg gagctcgagc gcgtccaggc ctggggcgac ttcgagcccg gcctcgccgc
     1921 cgagctcaag gccgcgggcc tggggctccg cctctatgag gggccgctcg ccaggctcgc
     1981 ctccctcccc gagggcctcg agtacctccg cctcgccgcg ccccgcggcc gggccaggat
     2041 agccgtcgtc gaaaggggcg gctcgccggc cctgcccgcg gaattcgccg aattcgccct
     2101 gcccgctgag tcgcccgcgg ccctgcgcga gggcgtcgcc cgcgccgagg cccggaaggc
     2161 cgccgccgag gcgggcctcg cctccctcgc gccttccttg cccgccctgc aggcggccaa
     2221 ggcctggctc tccgaagccc ttctcttcga gcaggcgcgg tcgggcatgg gagccgagga
     2281 gggcctcgcc ttcctttccg gattcgcgcc ggagcgcgag ctccccgccc tgaaggccgc
     2341 cgcggccagc catggctggg ccctgctctc ggacgacccc gcgccagagg aggcgccgcc
     2401 gagcaaggtg gaaaacggcc ccttcgtgcg catgatcgcc cccgtcttcg acttcctggg
     2461 gacggtgccg aactaccgcg agtacgacat ctcggggctg ttcctcttct tcttctgctt
     2521 cttcttcgcg atgatcttcg gcgacgcggg ctacggctcc atcctcctcg ccgcggggct
     2581 cttcgccgcg ctcaaggcca ggaaggccca gggcaaggtg cccgacccga tccgcctcct
     2641 cctcctcctc gcggccagca cggtggcctg gggcgtgatg acggccagct ggttcggcat
     2701 gccgccggag aggctccccg ccgtcttgcg ggccgtcgac atctactgga tctccaacga
     2761 gaacccggcc tctggcgaca acgtgaagat gctctgcttc ctcatcggga cggtccagct
     2821 cgtcgtggcc catctcaaga acatcaagcg ggacataggc tcgctcaagt tcctggccca
     2881 gatcggccag ctctccatgg tcgcgggcat gctcttcctg gtcctgaacc tcgtcatcag
     2941 ctccgagcgc ttccccctcc cgacctgggc cctctacctc gtcgcgggcg gcttcggcct
     3001 caactttgtc ttcgcgagct acgacgggtc caagggcctc gtgaagggca tcgtgggcgg
     3061 cgtcgtctcc agccttgcga acatcgtctc ggtcttcctg ggggtggtca acgtcttcgc
     3121 ggacatcgtg tcctacatcc ggctctgggc ggtcggcctc gcgggcatcg cgatcagcca
     3181 gacggtcaac aacatggccg gcccgatgct cggcagcctc atcatgttcg gggcgggggt
     3241 cctcctcctc gtcttcggcc acggcctcaa catcatgatg agcgtcctct ccgtcatcgt
     3301 ccacggcgtg cggctcaaca tgctcgagtt ctccggccac ctgggcatgg agtggtcggg
     3361 ctacaagtac gaacctttca gggccacggc gcccgaagaa cgactaaagg gataaggaaa
     3421 ggagaccagc atgaatatcg gtatgatcgg cgtcgcggcg gtcctcggcc tctcggccat
     3481 cggctcggcc atcggcgcgg gcatcgcggg ccaggcggcc atcggcgcgt ggaagaggaa
     3541 ctacatggcg aacaagcccg cctccttcct cctcgtcgtc ttcgcgggcg cccccctcac
     3601 ccagaccatc tacggcttca tcctcctcca gaggatgctc gcctccacga aggatcccct
     3661 cctcctcctg ggcgccggca tcatcggcgg cgcggcgatg ggcatgtccg ccatcgccca
     3721 gggcaaggcc tccgcctcct cctgcgacgc cttcggcgag accggcaagg gcttcggcca
     3781 gtacatcacc gtcgtcggcc tctgcgagac ggtcgccctc ttcgtgatgg ccttcacctt
     3841 cggcgccatc taggcttttc ggctccgagg aagcggccgc cccgagaggg cggccgcttc
     3901 gttttccgcg gatcgccgcg gcccccgaaa gcggccccgc gttccgcgcc ggcgccgcgg
     3961 ccgcgcgccg gccgccggcc cggcggcaac tccccccgcg tacgacggcc gcgccgacag
     4021 cgctcgcgcc gtcttgaacg gccagcctga gacggccttg cgcgcggggg ccggccttaa
     4081 gaccgcttcc actctggccg ccggccgcgc tagaatggcc ccatgcccag cactcgttca
     4141 gtcaaggcca agggcctcat catcggcggg ggcttccccg tctccatcca gaccatgtgg
     4201 aaggagcccc tccgcgcgga cgggctcgag gcggcggccg cgaggatagc ctccctcgcc
     4261 gagcttggct gcgacgtcct ccgcttcgcc gtgccggacc tggacgcggc ggaggtcctg
     4321 ggcaggctcg ccgccatgac ggagatgccc ctcgtcgccg acatccactt cgactggcgc
     4381 atagcgctgg cctgcctgga ccatcccata gccaagatcc gcatcaatcc cggcaacata
     4441 ggcgagcgct ggaaggtcga ggaggtcgcg aggaaggccg ccggccaggg cgtccccatc
     4501 cgcatcggcg tcaacggcgg ctccttgccc caggacctgc gggcccggat cgaccgcggc
     4561 gagctggacc gggccggcgc cctcgtggag gccgccgagc gggaggtcgc cgccttcgaa
     4621 gagctcggct tcgccgccct cgtcgtctcg atgaaggcga gctccgtgga ggagaccctg
     4681 gaggtcaacc gccgcttcgc gaagctccgc gactatcccc tccacctggg cgtcaccgag
     4741 gcggggcccc tcatcccggg catcgtgagg aacacggccg ccctcgtgcc cctcctcaag
     4801 gaagggatag gcgacaccat ccgcgtctcc atctccgaca cgatggagag cgaggtcgcg
     4861 gccggccgcg agatcctcgc ggcggcgggg ctggggaggg agggcgtcac cctcgtgtcc
     4921 tgcccccgct gcgccagggc cggcttcgac acccacgcct tcacggccaa gtgggcaagc
     4981 cggctctacg cgatgaagaa gagggccacg gtggcggtga tgggctgcgt cgtgaacggc
     5041 cccggcgagg cgcgacacgc ggacttgggc atcaccggag cgggcgacaa ggtcgtgatc
     5101 ttcaggaagg gaaagatagc gcgcacgata tcccgcgaag aagccgatac tgtattcgaa
     5161 gaggagcttc gcgcgctcga atgaaacagc accgcccgcc gatgtcgcaa gccttcgccc
     5221 tcgccgcctc ggccctgctc tgcgccgccg ccccgtccgc ggcagcccag tccgccgccc
     5281 cgcgggcggc cgccctgtca gcggcgcccc aggcggccgc cgcctccggc ccgggcggag
     5341 tctcccgcgc ggccgccggc gccgacgagt cctggctccg cttccagtcg gggaagcgcc
     5401 tcttcgagga gcgccgcttc ggcgaggcgc tcaacgcctt cgaggacgcc gccgccttga
     5461 ggcgcgaccg cttcgcctgg gcccagggcc gcctggacgg cgtcctttcg ggcgaggagg
     5521 ccagggccgc caaggactat cttccggccc tcgtggacag tctggccaag cgcgacctca
     5581 tcgagcggga ctacgcccag gtgaagaagg cctcgggagg ctccttgagg cgggaggccg
     5641 agctcctgcg gcagcggcgg ctctccgacg gcttcgccgg cttcctcgac gccctcgtcc
     5701 tcgtcctcga ccaccgtccc gccgagtccc tgggcggctc catcgcgaag ctccgctcgg
     5761 cggccgccga gctctcctcc tatccggagg ccgagtactg gatcggccgg gtctacctag
     5821 ccgaggggga gctccgcctc gccgagctcc agttccgcaa ggcctacgac cagcgcgagt
     5881 ccctggacat acccgaggac cgcttccaga tcctcgacgc cctggccggc gtctaccgcg
     5941 acggcggcaa gtggcgggac tacgaggaga gcctcaagga gatcgtcgcg gcagacccag
     6001 ccttctccga gaagagccgc ttcttccgcg acgcgatgga gcgcagcgtc cggcgggacg
     6061 ggatcgacaa gttcatgacc ctctaccgcg tcgagggcgg cgcctggacc aaggccggcg
     6121 ccgagctcgg gagcttctac ctcgcgaacg gccggcccca ggccctggtc cacttggccc
     6181 tggcggcgaa cggcctcctg gcccgctgca tcgagcgggt gcgggagcgc gagccttcct
     6241 acgcctatac gagcctccgg gagctcctcg ggaggatcga cgccgaccgc gagctccggc
     6301 gctacgcaga ggaggccaag ctctaccgct gcctctacga gctaggcgag gccctcgcgg
     6361 ccgaaggcga gagagattcc gcccggggca tctgggcggc gctcgcgggt cgcgatacgg
     6421 ccgctccctg ggacaggcgc tcggcggagg ccctggcccg agccttccgc tagagggtcc
     6481 cggcggcgcc gcgtggccgc ctttggcttc gtccagcggc gcctagcaga ggtctggaac
     6541 gggcacgcgg cggtctggcg tcgccaagcg gcgtcctctc ggcggcctat cgcaggccct
     6601 tctccagggc gcggagcgcg acggcctcgg gcgtctcgac cagggcgacc gagcattctt
     6661 ccttgtacag cacgagcctc aggcttcccg gaaggccctt tttgtcgcgg cgcatggccg
     6721 cgaggagggc ctcgggctct attccggaat agctcgtctc gaagccgtag gcctcgatga
     6781 gggagcgggc ctcggcggcg gcctcgggat cggagaggcc gagctccagg cccgcgtcca
     6841 gggcgcgggc catgccccag gcgactgcct cgccgtggag gatgtcgggt cgcagggctt
     6901 ccagggcgtg ggcgaaggtg tggccgaagt tgaggcggag gcggtcgccg cggtcgtagg
     6961 ggtcgcgctc gcagagggag gccttcgcga ggacgcatct cctcaccgtc tcgtctagga
     7021 gggccaggtc gcgcgaccgg aggtcggccc cgcgtccgga aaggaggctc cgcaccttgg
     7081 gggcggcgac caggcccgtg cggaggacct ccgcgaggcc cgagcggtac tcccgctcgg
     7141 ggaggctcgc gaggaagggc gtgtggatcc gcacttcgtc ggccgcgtag aaggtcccgg
     7201 ccaggttccg gacgccggcc aggtcgaccg cggccttgcc gcccagggcg gcgtccgcca
     7261 tcgcgacgag gctcgtcggg acgaggacga ggcgggagcc ccgcatgtag agggaggccg
     7321 cgaaggcggc gaggtcgcag gggacgccgc cgcccaggcc caggatggct ccgtcccgcg
//
LOCUS       DAOOSJ010000131         7369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6275_length_7369_cov_5.510118, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000131 DAOOSJ010000000
VERSION     DAOOSJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6275_length_7369_cov_5.510118"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLG14_07545"
     CDS             <1..464
                     /locus_tag="PLG14_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyl-tRNA--protein transferase"
                     /protein_id="HOX49028.1"
                     /translation="ASYSEERFGLSRRSLKERHGFRSGDDSGDEARAAYRNFLVESPV
                     PGTSIAEYRLPDGRLAATGYLDVLPGGLSSVYFAFDPKESRRSVGTWSVGRELELAAS
                     LGKRYYYLGFWVPGSEKMDYKADFRPFEFALDGAWRPARDRSEALAALESA"
     gene            461..2644
                     /locus_tag="PLG14_07550"
     CDS             461..2644
                     /locus_tag="PLG14_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX49029.1"
                     /translation="MSEASDRIDAANAKAWDLMLADPEGALAVAERALVEAEEQGYQA
                     GLAEAELNVGWCESYLTRPGPAISTFEKALAAFSALGDWLGTMKTLNALGVAYHNLGR
                     YDRAMDYYTRSLEEARRAGNSFREASTLNNIGEVCLDLGEHKEALDYFLRAYETVPDD
                     SDTEVVSNILLNIGTTFHRMENWPLAREFTEKALVIAEEADDRLIAAQCWLSLGSIDA
                     SSERYTEAEGHLLKALALDEALKNEKQRVDVLLELGSLSLGLGDPAKARERFEDARAR
                     AEALGAKAQINEAYKRLAEAHERLGDFRTALEHFKRFSSYERETQGEDTSRKIKNVTV
                     QYEVEKSRQEAEIYRLRNIELRDKTAELEEANRQIVSISELGRRITSSLDLDTVVQTL
                     YESLERHLDVTVFGIAVHDQDEGTLEWRIFIEKGKRIQRAPQKLDFQRSFAGYCIKRR
                     ETVFMNDGEAEYTRYIEGKRISHGTPAESLVFIPLTIEDRAIGVLTVQSYSKNAYSGK
                     NRVLLEALAPYVAIAIENGLIHDRLEELNRAISSEKSELEKAAKRISHLANHDSLTGL
                     PNRRLLFELLQKSFDIAGRSGSRVGVLYIDLDDFKPINDRYGHFAGDRALVAVAERLS
                     GILRASDTLARVGGDEFIAVLTNVRDRSDIELVARKILEGCLEPLPLPGMDCRVGLSM
                     GIAVYPDDSRSIEELVSLADASMYRVKRERKHGFSFYSEEPGATA"
     gene            2948..4510
                     /locus_tag="PLG14_07555"
     CDS             2948..4510
                     /locus_tag="PLG14_07555"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX49030.1"
                     /translation="MASQTPNAPAARTPKKDNADAPKRGIKNPFIYVGTIVLLVIVVV
                     AFVFVPSVGSFDSSGKALQFGSYAGKAIAYEQGNYFSRQVQTVNDALRQQGLSEQNFQ
                     FFAYQVWRQAFERTVYRMAVLDEVKSAGGFVTEARLDARMAENPAFQEDGKFSARRYR
                     EASMSEKLSLRDALRDDLLSDQYLSEVYGLQPSSKEIAFVKDMGKETRTIEYAVFPLS
                     SFPDSEVAAWASQNAALFRRLKLSRITVSSSEADALKVRKQVESGGVSFEDAAKGHSK
                     DSWADKGGLVGARYFHEIAGDLESKEAAEKLAALKKGELSPVLKTITGSWVFFKADEA
                     FVPADAADPAFLKDARAYVLSNERGKVEDYVAAKAKDFAAAAAKDFAAAGRASGVEAK
                     AAGPFPLNYGDLDFSIYGQTVPLFKRVNADSAPELSGAETSEKFLGAAFSLAPGAVSE
                     PLVLGDNVLVLKVKEAGAALDEDLSGISLYYPYFFQQKTDGELRGLFLKSPKLKDNFM
                     NVFFKYFSPKQS"
     gene            4514..>5814
                     /locus_tag="PLG14_07560"
     CDS             4514..>5814
                     /locus_tag="PLG14_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOX49031.1"
                     /translation="MDADGPALVETGGALNVRYRGRLLYSERGPALIPGRAALACELG
                     PARLYLVSSPCLWYGIPELLARLEPGSAVLCLEADEALARLSLERAPAGLLDDPRLAF
                     LPSADPAKALEAAAALGSFRRCVPLRLSGGDAFHADEYRRAAAVLQAEFAAAWRNRAA
                     LLSMGRLWARNIFRNLASLREIGPEAPPRCEGAAFVCGAGPSLEASLPLIAARRGRLA
                     LIACDTALPVLLGAGLEPDLVVCLEGQAHNLADFTPAGGREIPLLADLSSHPATFRAL
                     RGRKHLSLVHIAPGAFMRRVSGLGLPCVELPPLGSVGVHAVHLARKLASGPVLAAGLD
                     FSYELGKTHARGAPALLSEERRMTRLYRSRAQLAAAFKPGARPAPGAGAAVRATAVTT
                     TVAADGASGQAPAPGARAGAPLTDPVLSSYAALLAEENAGPG"
     assembly_gap    5815..5914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5915..6429
                     /locus_tag="PLG14_07565"
     CDS             <5915..6429
                     /locus_tag="PLG14_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49032.1"
                     /translation="PVLSSYAALLAEENAGPGPLILDLRGRGLPIGGRPTSLDEADRL
                     LAALEPSGLGAGDRPGSGSGTDKAAAARAAAEAADRAEAFLAEEADRLERVKDALRGG
                     SGRSGGELASLVAGSDYLLWPFPDADRFGGLPQDLLNRLLVEVEYWRWKLGEIRAGSR
                     AFRSRGPSGD"
     gene            complement(6426..7202)
                     /locus_tag="PLG14_07570"
     CDS             complement(6426..7202)
                     /locus_tag="PLG14_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49033.1"
                     /translation="MRTVRSRPRPAKSGALAAASLLAAAFSFLLPGCARPGPRPPETL
                     VSKDGAYIVVIHEGVEAEEAKSVLEALEAQAPRIKADLGVSDPVPYSVHLRGSVGEYL
                     EVMRGLIGGVFPGSTGYAAGRHRAEILLTPLAAQDAVHEYAHSVSLAWARNWHNKPRW
                     LWEAVALYEAGELVDPRSLPYLSEGEFPSLAELDEDYNSSDHRIYEVGYLLLDFLRAR
                     WGMEKVKELVRSTGDIEGVLGVAEEDFERDWAAFVRKKYL"
BASE COUNT         1113 a         2758 c         2379 g         1019 t
ORIGIN      
        1 ccgcctccta ttcggaggag cgcttcggtc tctcccggcg ctccctgaag gagcgccacg
       61 gcttccgctc gggcgacgac tcaggcgacg aggcgcgcgc ggcctacagg aacttcctcg
      121 tcgagagccc ggtgccgggc acctcgatag ccgagtaccg cctccccgac ggcaggctcg
      181 ccgccaccgg ctacctggac gtcctccccg gcggcctctc gagcgtctac ttcgccttcg
      241 acccgaagga gtccaggagg agcgtgggca cctggtccgt cgggcgcgag ctcgagctgg
      301 ccgcctccct gggcaagcgc tactactacc tcggcttctg ggtgcccggc tcggaaaaga
      361 tggactacaa ggccgacttc cggcccttcg aattcgccct ggacggggcc tggcgccccg
      421 cccgcgaccg gagcgaggcc ctcgcggccc tggagtccgc gtgagcgaag cctccgaccg
      481 catcgacgcg gccaacgcga aggcctggga cctcatgctc gccgatcccg agggggcgct
      541 cgccgtcgcg gagcgcgccc tggtggaggc cgaggagcag ggctaccagg cgggcctcgc
      601 cgaggccgag ctcaacgtcg gctggtgcga gagctacctg acgaggcctg gcccggccat
      661 ctccaccttc gagaaggccc tcgccgcctt ctccgccctg ggcgactggc tcggcacgat
      721 gaagaccctc aacgccctgg gcgtcgccta ccacaacctg ggccgctacg accgggcgat
      781 ggactactac acgcgcagcc tcgaggaggc gcgccgcgcc ggcaactcct tccgggaggc
      841 ctccaccctc aacaacatcg gcgaggtctg cctggacctg ggcgagcaca aggaggccct
      901 cgactacttc ctcagggcct acgagaccgt gcccgacgac tccgacaccg aggtcgtctc
      961 caacatcctc ctcaacatcg ggacgacctt ccaccgcatg gagaactggc ccctcgcgcg
     1021 ggagttcacc gagaaggccc tcgtgatagc ggaggaggcc gacgaccgcc tcatcgccgc
     1081 ccaatgctgg ctctccctgg gcagcatcga cgcctcgtcg gagcgctaca cggaggcgga
     1141 gggccatctc ctcaaggccc tggccctgga cgaggcgctc aagaacgaga agcagcgcgt
     1201 cgacgtcctc ctggagctcg gctccctgag cctgggcctg ggcgaccccg cgaaggcgcg
     1261 ggagaggttc gaggacgcgc gcgcccgcgc ggaggccctg ggggccaagg cccagatcaa
     1321 cgaggcctac aagcgcctgg ccgaggccca cgagcgcctg ggcgacttca ggacggccct
     1381 ggagcacttc aagcgcttct cgagctacga gcgcgagacg cagggcgagg acacgagccg
     1441 caagatcaag aacgtcacgg tccagtacga ggttgagaag agccgccagg aggcggagat
     1501 ctaccgcctg cgcaacatcg agctcaggga caagaccgcc gagctcgagg aggccaaccg
     1561 ccagatcgtc tccatctccg agctgggacg ccgcataacg tcaagcctgg acctggacac
     1621 ggtggtccag accctctacg agagcctgga gcggcacctg gacgtgaccg tcttcggcat
     1681 cgccgtgcac gaccaggacg agggcacgct cgagtggagg atcttcatcg agaagggcaa
     1741 gcggatccag cgcgcgcccc agaagctcga cttccagcgg agcttcgccg gctactgcat
     1801 caagcgccgc gagaccgtct tcatgaacga cggcgaggcc gagtacacga ggtatatcga
     1861 gggcaagcgg ataagccacg ggacgccggc ggagtccctg gtcttcatcc ccctcaccat
     1921 cgaggaccgc gccatcggcg tgctcaccgt gcagagctac tccaagaacg cctacagcgg
     1981 gaagaaccgc gtcctcctcg aggccctggc gccctacgtc gcgatcgcca tcgagaacgg
     2041 cctcatccac gaccggctgg aggagctcaa ccgcgcgatc tcgagcgaga agagcgagct
     2101 cgagaaggcc gccaagcgca tcagccacct ggccaaccac gacagcctga cggggcttcc
     2161 caaccggcgc ctcctcttcg agctcctcca gaagtccttc gacatcgcgg ggcggagcgg
     2221 cagccgggtg ggcgtcctgt acatcgacct ggacgacttc aagccgatca acgaccgcta
     2281 cggccacttc gccggggacc gggccctcgt cgccgtcgcg gagcgcctct ccggcatcct
     2341 gcgcgcctcc gacaccctgg cccgggtggg cggcgacgag ttcatcgccg tgctcacgaa
     2401 cgtgcgcgac cgctcggaca tcgagctcgt cgccaggaag atcctggagg gctgcctgga
     2461 gcccctgccc ctcccgggca tggactgcag ggtcggcctc tccatgggca tcgcggtcta
     2521 ccccgacgac agccggagca tcgaggagct cgtgagcctc gccgacgcct cgatgtaccg
     2581 cgtgaagcgc gagcggaagc acggcttctc cttctactcg gaggagccag gcgcgactgc
     2641 ctaggggtcc cggcgcgggc cgccggccgc gacggcgcag ggccgtcggg ccctgcgagc
     2701 ccgggtcctg cgagcccggg actcgcgagt ccggaccctg cgagcccggg ccccggcggg
     2761 gcggcccccg gcagccgcct cgtcgttgac agcctaattc cagcgtggta tggttccccg
     2821 ataccgtagc ccgggggctg cgaaccgccc tagggcggcc ccctccccgg cgggtcgaag
     2881 cggaaggttt tccgtggcgc cccccggccc ccggcccggc agcacagctc cgaaacggaa
     2941 ggaagccatg gcatcccaga caccgaacgc gcccgccgcc cggacgccca agaaagacaa
     3001 cgccgacgcg cccaagcgcg gcatcaagaa ccccttcatc tacgtcggca cgatcgtcct
     3061 cctcgtcatc gtcgtggtcg ccttcgtctt cgtgccctcc gtcggcagct tcgacagctc
     3121 cggcaaggcg ctgcagttcg gcagctacgc cgggaaggcc atcgcctatg aacagggcaa
     3181 ctacttctcc cgccaggtcc agacggtgaa cgacgccctc cgccagcagg gcctctcgga
     3241 gcagaacttc cagttcttcg cctaccaggt ctggcgccag gccttcgagc gcacggtcta
     3301 ccggatggcc gtcctcgacg aggtgaagtc ggccggcggc ttcgtcaccg aggcccgcct
     3361 ggacgcccgc atggccgaga atcccgcctt ccaggaggac ggcaagttct ccgcccgccg
     3421 ctaccgcgag gcctccatgt cggagaagct ctcgctccgc gacgccctcc gcgacgacct
     3481 cctctccgac cagtacctct ccgaggtcta cggcctccag ccgagctcca aggagatcgc
     3541 gttcgtgaag gacatgggca aggagacgag gacgatcgag tacgccgtct tccccctctc
     3601 ctccttcccc gactccgagg tcgccgcctg ggccagccag aacgccgccc tcttccgacg
     3661 cctcaagctc tcccggatca ccgtctcctc cagcgaggcc gacgcgctca aggtccgcaa
     3721 gcaggtcgag tccggcggcg tctccttcga ggacgcggcc aagggccact ccaaggacag
     3781 ctgggccgac aagggcggcc tcgtcggcgc ccgctacttc cacgagatcg cgggcgacct
     3841 cgagagcaag gaagccgccg agaagctcgc cgccctcaag aagggcgagc tctcccccgt
     3901 cctcaagacc atcaccggct cctgggtctt cttcaaggcc gacgaggcct tcgtccccgc
     3961 cgacgcggcc gacccggcct tcctcaagga cgcccgcgcc tacgtgcttt cgaacgagcg
     4021 cggcaaggtc gaggactacg tcgcggccaa ggccaaggac ttcgccgccg ccgcggccaa
     4081 ggacttcgcc gccgcgggca gggcctcggg cgtcgaggcc aaggccgccg gccccttccc
     4141 cctgaactac ggcgacctcg acttctccat ctacggccag accgtgccgc tcttcaagcg
     4201 cgtgaacgcc gactccgcgc ccgagctctc cggagccgag acgagcgaga agttcctggg
     4261 cgcggccttc tccctcgcgc ccggcgcggt ctccgagccc ctcgtgctcg gcgacaacgt
     4321 cctggtcctc aaggtcaagg aggccggcgc cgccctggac gaggacctct cgggaatctc
     4381 cctctactac ccctacttct tccagcagaa gacggacggc gagctccgcg gcctcttcct
     4441 caagagcccg aagctcaagg acaacttcat gaacgtcttc ttcaagtact tcagccccaa
     4501 gcagtcctaa gccatggatg ccgatggccc cgcgctcgtc gagacgggcg gggccctcaa
     4561 tgtccgctac cggggccgcc tcctctattc ggagcgcggc cccgctctca ttcccggccg
     4621 cgccgccctg gcctgcgagc tcggccccgc ccgcctctac ctcgtctcct ccccctgcct
     4681 ctggtacggg atccccgagc tcctggcccg cctggagccg ggaagcgccg tgctctgcct
     4741 ggaggccgac gaagcgctcg ccaggctctc gctcgagcgg gcgcccgccg gcctcctcga
     4801 cgatccgcgc ctcgccttcc ttccgagcgc cgatcccgcg aaggccctgg aggccgccgc
     4861 cgccctcggc tccttcaggc gctgcgtgcc cttgaggctc tccggcggcg acgccttcca
     4921 cgcggacgag taccgcaggg cggcggccgt cctccaggcc gagttcgcgg ccgcctggag
     4981 gaaccgcgcc gccctcctct ccatggggag gctctgggcc cggaacatct tccgcaacct
     5041 ggcttccctg cgggagatcg ggccggaggc gcctccgcgc tgtgagggcg ccgccttcgt
     5101 ctgcggggcg gggccttccc tggaggcctc cctccccctc atcgcggcga ggcgcggccg
     5161 cctggccctc atcgcctgcg acacggccct ccccgtcctc ctgggcgccg gcctcgagcc
     5221 agacctggtc gtctgcctgg agggccaggc gcacaacctg gccgacttca cgccggccgg
     5281 aggcagggag atacccctcc tcgccgacct ctcctcccat cccgcgacct tccgcgcgct
     5341 ccgcggcagg aagcacctga gcctcgtcca catcgccccc ggcgccttca tgcgccgcgt
     5401 ctcgggcctg ggcctaccct gcgtcgagct ccctcccctg gggtcggtgg gcgtgcacgc
     5461 ggtccacctc gcccggaagc tcgcctcggg gcccgtcctg gccgcgggcc tggacttctc
     5521 ctacgaactc gggaagaccc acgcccgggg cgctcccgcc ctcctttcgg aagagcgtcg
     5581 catgacgcgg ctttaccgct cgcgcgccca gctcgccgcc gccttcaagc ccggcgcccg
     5641 gcccgcgccg ggggcgggtg ccgccgtccg ggcaacggcc gtcacgacga ccgtcgccgc
     5701 ggacggggcc tccggccaag ctccggcccc gggagccagg gccggggcgc cgctcaccga
     5761 tcccgtcctc tcgtcctacg cggccctcct cgccgaggag aacgccggcc cgggnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcccg tcctctcgtc ctacgcggcc
     5941 ctcctcgccg aggagaacgc cggcccgggc cccctcatcc tcgacctccg cggccgcggc
     6001 cttcccatcg gcgggcggcc gacgagcctg gacgaggccg accgccttct tgcggcgctc
     6061 gagccttccg gcctcggcgc cggggacagg ccaggaagcg gctcggggac ggacaaggcg
     6121 gccgcggcgc gggctgcggc cgaggcggcc gacagggcgg aggccttcct ggcggaggaa
     6181 gccgacaggc tcgagcgagt gaaagacgcg ctgcgcggag gctcgggccg gagcggcggg
     6241 gagctcgcct ccctcgtcgc gggctcggac tacctcctct ggcccttccc cgacgccgac
     6301 cgcttcggcg gcctgccgca ggacctcctc aacaggctcc tcgttgaagt ggagtactgg
     6361 aggtggaagc tcggggagat cagggcggga agccgggctt tccggagccg cggccccagc
     6421 ggagactaga ggtacttctt cctgacgaag gcggcccagt cgcgctcgaa gtcctcctcc
     6481 gcgacgccga ggactccctc gatgtcgccc gtcgagcgga caagctcctt gaccttctcc
     6541 atcccccagc gcgcgcggag gaaatcgaga aggagatagc ccacctcgta gatgcgatgg
     6601 tcgctcgagt tgtagtcctc gtcgagctcg gcgaggctcg ggaactcccc ttcggacagg
     6661 tagggaagcg agcgtgggtc cacaagttcc ccggcctcgt agagggcgac ggcctcccag
     6721 agccagcggg gcttgttgtg ccagttcctc gcccaggcga gcgagacgga gtgggcgtac
     6781 tcgtgcacgg cgtcctgggc ggccaggggg gtgaggagta tctcggcccg gtgcctgccc
     6841 gcggcgtagc cggtcgagcc ggggaagacg ccgcctatga gcccccgcat cacctcgagg
     6901 tactcgccga cggagccgcg caggtgcacg gaatagggca cgggatcgga gacgcccagg
     6961 tcggccttga tgcggggcgc ctgggcttcg agggcctcga ggacggactt ggcctcttcc
     7021 gcctcgacgc cttcgtggat gaccacgatg taggcaccgt ccttggagac gagggtctcg
     7081 ggcggccggg ggcccgggcg ggcgcagccc gggagaagga aggagaaggc ggcggccagg
     7141 agggaggcgg ccgccaaggc gccggacttg gccggcctcg ggcgagatcg gactgtccgc
     7201 atggaacctc cctggccctt gcgcccttcc gcgcaagacc cgccttgcgc ctcgggagcc
     7261 tagcgctcct tctcctcgtt ctcgcgcagg acccgtcctg cgcctcggaa gtctagcgct
     7321 ccctctcctc gttctcgcgc aagacggcca ggaaggcctc ctgcggaag
//
LOCUS       DAOOSJ010000132         7365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6281_length_7365_cov_5.448427, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000132 DAOOSJ010000000
VERSION     DAOOSJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_7365_cov_5.448427"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..645
                     /locus_tag="PLG14_07575"
     CDS             247..645
                     /locus_tag="PLG14_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX49034.1"
                     /translation="MNRTIAERGLSFAIDPASGVPFYRQIIQQIEHAVLSGRLEPGDR
                     LPTIRALAIELKINPNTIAKAYGELEIRGILLTQVGSGTFVSDKRASPGESERAKRLE
                     ERTARFIRDMETLGADRTTMVELVRDFKED"
     gene            648..1760
                     /locus_tag="PLG14_07580"
     CDS             648..1760
                     /locus_tag="PLG14_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOX49035.1"
                     /translation="MWFAKKQAEAPLVKNAKKPRPRRDFAVGSAGIGLLAAIVAFTVA
                     ARLLSGSSPSARDLGLLLGAPVAAAVAMTLFPRWSAALKVLLIAALALAAWRFDEWGL
                     GLALAAAGTLAAPAVQKLEEWERAVVLRFGRFHRLRGPGLFPLLPIADRVASVVDLRI
                     RVTDFAAETTLSRDSVTVTVDALCFWLVWDAEKAVLEVQDYEEAVILSSKTALRNAVS
                     KNDLSAFLERGDDIEEQIRAEVDRKTTEWGITVQHIEITDIQIPEGLQDSMSRLAQAE
                     REKRGRILLAEAEVEMARKFEEAAKVYGSNATALRLKSLSVLNEGLKAGNSMMLVPDS
                     LAAELKTRDLFGLEALAEGRRAAKEDKEADDERRQD"
     gene            1741..2424
                     /locus_tag="PLG14_07585"
     CDS             1741..2424
                     /locus_tag="PLG14_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49036.1"
                     /translation="MSVVRTEGLVKDYALNGITVNALRGVDLSFEPGEFAAVAGPSGS
                     GKSTFLHLVGCLDRPTGGRISIDGADAAGLSRKELALLRRKRIGFIFQAYNLIPVLTA
                     LENVSLPLTLLGEGAASARAKAERALEEVGLADYGKRRPKEMSGGQQQRVAIARALVK
                     RPALILADEPTANLDSKTGKEILELMLGLNAGEGTTFIFSTHDKMVMEYARRLVMLRD
                     GLVESETRK"
     gene            2421..4148
                     /locus_tag="PLG14_07590"
     CDS             2421..4148
                     /locus_tag="PLG14_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX49037.1"
                     /translation="MTRAGFRDIAAMSLRNLSRHRTKTVITTLAVAVSVALYIFVDAW
                     LLGMNLDSRRNIVSYEIGAAKIQSRAYFDKKDELPMYESFPGWEGLSGALAAKGYDSA
                     PRFVFSGTLYSRSGTAPMVFNAVDPGREARLLRYPAFLEAGRFPEAGKPELALGALAA
                     EKLKVGIPLRPTKAELEELAAAAPTGEDAAFVLSLYGPPPAKAKGAKTSVFENKDPAR
                     AAAEAAKLALKEGATKAELDRLWAILAASGRMDVRIATTIDLKAAPESIRGAKFEGEL
                     LPALGPEARARALAAYEKDPLTGDYRLAAPGTAEAGAVQDDMAASGFSGAIRHVNQLV
                     DAVVVGVVNSPNPKTNGNVGFIPLDALQDESGLMLAGRVTELLVRKAGADDSALPGKD
                     ESPEAIAAAVAAELPEGLAVRGWRDYVADFLAAYNGDNVSTRLMVFFLFLLSFIGIAN
                     TMLMAILERTKETGMLRALGMTDGEVLLAYVLEAGLIGAIGSAFGVAAGCLMNIPMVA
                     QGIDYSAVSKEMGGDFGYRITTLFKSAWNPLAIALTGVGATLLSAAAAVPPSLRALRM
                     PVTDSLRFE"
     gene            4157..5434
                     /locus_tag="PLG14_07595"
     CDS             4157..5434
                     /locus_tag="PLG14_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49038.1"
                     /translation="METSNGAGVGGLWALAVIAYRNVWRNGRRTALCVVAVAIAVFFN
                     IFMRSWIEGMMDGIEEAVRTFETGHVNAFTERFEAEREYLPVQYPLDEGRSSDELVAE
                     IEALPGVEAALPRISAYATLFDSTVKHAVLWGVDPARETKVNRLNLTDRDEGLVEGRF
                     PALGANECAIGAETARKTGLRIGDKLPLKTVSAQFSDKYWSPTVVGVFEFDYAKYDDG
                     AVIVPIDRLRRVLALGEATQGIFVYARDAEDSPAIKAGVERILGPGHVVRQWTDNYWV
                     AVMRQSSYIYAIVFAVFQVVASFLIINTVLMVIHERIKEIGMMGALGMTRREIVLVFF
                     FESVVLSVLGSLAGCALGAAATAIGSNYPIDMELFTGGGMKEMPISGTLFLAFSPGAI
                     VGGFGFGVLVSAACTLLPSLKSAFIEPVEALRR"
     gene            5602..6330
                     /locus_tag="PLG14_07600"
     CDS             5602..6330
                     /locus_tag="PLG14_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX49039.1"
                     /translation="MSAGAFPAPVFGQAAGGQPSAAALLERVDANEVYSSIAYEGEMA
                     IEYQGKRFVKRFKAFARGGSESFIEFTNPEDAGTKYLKREGRLYVYSPDAEEVMLISG
                     HLLKESMMGSDLSYEDTIENEKLSVRYEPSLAGQEEASGRLAYVLDLRARRSGESYPR
                     QKLWIDAQTGDLLRAEYYALSGAKLKEYALLRAETIGGRRFPVESEMRDLLRKGSRTV
                     FRMSAVRLDAPIPDETFSVRNLER"
     gene            6327..>7365
                     /locus_tag="PLG14_07605"
     CDS             6327..>7365
                     /locus_tag="PLG14_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49040.1"
                     /translation="MRRQPRAAALALAALLASLAAALPAAAEGPAISGSIETSAAASL
                     GAGASPAFSLGFEEYANLRLKAKAGERGTVHAALNLVAASGSLAPPEAVLAAAPYATP
                     FVSGGGYAAALELERLYCRITGETMDLEAGLLRLAFGYGQAFSPMDFLSARNPLLPEA
                     RPRGVLGVAASAYPSDLARIEAFAAAGRDPFETSGEGALVGALGELHGRSASLETLYA
                     YEAPSEAYGRGVHRAGLSLKLEAGAGIVLDALYSHDGGERSGLGGLEGSLGLDYSLGG
                     GDLYLLCQYLYRGPGSVGDLTGGNYLYAAASWRVDDYRSISLASTANLDDLSALRFLS
                     YETEPFQGLAVL"
BASE COUNT         1178 a         2639 c         2453 g         1095 t
ORIGIN      
        1 cgtggagtgg acctggctgg agcgatagga gcctgtccgt gaaacccctg aggccgcatc
       61 gacttgcgga ttcggctcgc tcaggctcgc cgaatccgcc cttcatccat cgcgcctgta
      121 ggttaagatc ttcaccatag atcccaggac aggctcctcg ctgcccgtag cctcccctaa
      181 caaaccagcg agaaatctcc tctccgctct tgacacattg tactagttca atcactattg
      241 tactagatga ataggacaat agccgagcgc ggtctctcct tcgccatcga cccggcgagc
      301 ggggtgccct tctaccgcca gatcatccag cagatcgagc acgccgtcct ctccgggcgg
      361 ctcgagccgg gagaccggct gccgacgatc cgcgccctcg ccatcgagct caagatcaac
      421 ccgaacacga tcgcgaaggc ctacggcgag ctcgagatca ggggcatcct cctcacccag
      481 gtggggagcg gcaccttcgt ctccgacaag cgcgcgagcc ccggggagtc cgagcgggcg
      541 aagcgcctcg aggagcggac ggcccgcttc atccgcgaca tggagaccct gggcgccgac
      601 aggacgacga tggtcgagct cgtccgcgat ttcaaggagg actagacatg tggttcgcta
      661 aaaaacaagc ggaggcgccc ctcgtcaaga acgccaaaaa gccgcggccg cggcgggatt
      721 tcgccgtcgg ctcggcgggc ataggcctcc tcgcggcaat tgtcgccttc acggtcgccg
      781 cccgcctcct ctcgggctcg agcccctcgg cccgggacct cggcctcctc ctgggggccc
      841 ctgtggcggc ggcggtcgcc atgaccctct tcccgcgctg gagcgcggcg ctcaaggtcc
      901 tcctcatcgc ggccttggcc ctcgcggcct ggcgcttcga cgagtggggc ctgggcctgg
      961 ccctggccgc ggcgggcacc ctggccgcgc ccgccgtcca gaagctcgag gaatgggaac
     1021 gggccgtggt gctccgcttc ggaaggttcc atcgcctccg gggccccggc ctcttccccc
     1081 tcctccccat agcggaccgg gtggcgagcg tcgtggacct gcggatccgc gtcaccgact
     1141 tcgccgcgga gacgaccctc tcccgcgact ccgtgaccgt caccgtcgac gccctctgct
     1201 tctggctcgt ctgggacgcg gagaaggccg tcctcgaggt ccaggactac gaggaggccg
     1261 tcatcctctc ctcgaagacg gccctgcgga acgcggtcag caagaacgac ctctccgcct
     1321 tcctggagcg gggcgacgac atagaggagc agatccgcgc cgaggtcgac cggaagacga
     1381 ccgagtgggg catcacggtc cagcacatcg agatcaccga catccagatc cccgaggggc
     1441 tccaggactc catgagccgc ctggcccagg ccgagcgcga gaagcgggga cggatcctcc
     1501 tggccgaggc ggaggtcgag atggcgagga agttcgagga ggcggccaag gtctacggct
     1561 cgaacgcgac ggcgctcagg ctcaagagcc tctccgtcct caacgagggc ctcaaggcgg
     1621 gcaactccat gatgctggtc cccgactcgc tggccgccga gctcaagacc cgggacctct
     1681 tcggactaga ggccctggcc gagggccggc gcgcggcgaa ggaagacaag gaggcggacg
     1741 atgagcgtcg tcaggactga agggctcgtc aaggactacg ccctgaacgg gatcacggtg
     1801 aacgcgctcc ggggcgtcga cctctccttc gagccggggg agttcgcggc cgtggcgggc
     1861 ccatccggca gcgggaagtc caccttcctc cacctcgtgg gctgcctgga caggcccacg
     1921 ggcggaagga tctccatcga cggcgcggac gccgccggcc tctcccggaa ggagctggcc
     1981 ctcctcaggc gcaagcggat cggcttcatc ttccaggcct acaacctcat tcccgtcctc
     2041 accgccctgg agaacgtgtc cctgcccctg accctcctgg gcgagggggc cgcgtcggcg
     2101 cgggccaagg ccgaaagggc gctggaggag gtgggcctcg cggactacgg gaagcggcgg
     2161 cccaaggaga tgtcgggagg ccagcagcag cgcgtcgcga tcgcccgcgc cctcgtgaag
     2221 cggccggccc tcatcctcgc ggacgagccg acggcgaacc tcgactcgaa gacgggcaag
     2281 gagatcctgg agctcatgct cgggctcaac gccggcgagg gcaccacctt catcttctcc
     2341 acccacgaca agatggtgat ggagtacgcg cgccgcctcg tcatgctccg ggacggcctc
     2401 gtggaatcgg agacgcgcaa atgaccaggg cgggcttccg cgacatcgcc gcgatgtcgc
     2461 tccgcaacct ctcgcggcac cggaccaaga cggtcatcac gaccctcgcc gtcgcggtga
     2521 gcgtggcgct ctacatcttc gtcgacgcct ggctcctcgg gatgaacctg gactcaaggc
     2581 gcaacatcgt ctcctacgag atcggagcgg cgaagatcca gtcccgggcc tacttcgaca
     2641 agaaagacga gctgcccatg tacgagagct tccctggttg ggagggcctc tccggggccc
     2701 tcgcggcgaa gggctacgac tcggctccgc gcttcgtctt ctcgggcacc ctctattccc
     2761 gctcggggac ggcccccatg gtcttcaacg cggtggaccc cgggcgcgag gcccgccttc
     2821 tccgctaccc tgccttcctg gaggccggcc gcttcccgga ggcgggcaag cccgagctcg
     2881 ccctcggcgc gctggccgcg gagaaactca aggtcggcat ccccttgcgc ccgacgaagg
     2941 cggagctgga agagctcgcc gcggcggccc cgaccgggga ggacgcggcc ttcgtgctct
     3001 ccctctacgg tccgcccccc gccaaggcga aaggcgcgaa gacgagcgtc ttcgagaaca
     3061 aggatcccgc ccgggccgcg gcggaggccg cgaagctcgc cctcaaggag ggcgcgacga
     3121 aggccgagct ggaccggctc tgggcgatcc tggccgcctc cggcaggatg gacgtccgca
     3181 tcgccacgac gatagacctg aaggccgccc ccgaatcgat ccggggcgcc aagttcgagg
     3241 gagagctcct gcccgccctg ggccccgagg cccgcgccag ggccctggcc gcctacgaga
     3301 aggatcccct gacgggggac tacaggctcg cggctccggg aacggccgag gcgggcgccg
     3361 tccaggacga catggcggcc tcgggcttct ccggcgcgat ccgccacgtg aaccagctcg
     3421 tcgacgccgt cgtcgtcggc gtggtcaact cgcccaaccc gaagacgaac ggcaacgtcg
     3481 gcttcatccc cttggatgcc ctacaggacg agtccggcct catgctcgcc ggccgcgtca
     3541 cggagctcct cgtgcgcaag gccggcgccg acgactccgc cctgcccggg aaggacgagt
     3601 ctcccgaggc gatcgcggcc gccgtcgcgg cggagctgcc cgaggggctg gccgtgcgcg
     3661 gctggaggga ctatgtcgcc gacttcctgg cggcctacaa cggggacaac gtctccaccc
     3721 ggctcatggt cttcttcctc ttcctcctct ccttcatcgg catcgccaac acgatgctca
     3781 tggccatcct ggagcgcacg aaggagacgg ggatgctccg ggcgctcggg atgacggacg
     3841 gggaggtcct tctcgcctac gtgctggagg cggggctcat cggggcgata ggctcggcct
     3901 tcggcgtcgc cgcgggctgc ctcatgaaca tcccgatggt ggcccagggc atcgactact
     3961 ccgcggtctc caaggagatg ggcggcgact tcggctaccg catcacgacg ctcttcaagt
     4021 cggcctggaa cccgctcgcc atagccctca cgggcgtggg ggccacgctg ctctccgcgg
     4081 cggcggcggt gccgccgagc ctgcgggcgc tcaggatgcc cgtgaccgat tccctgcggt
     4141 tcgagtgagg cgcgacatgg aaacaagcaa cggagccggc gtcggcggcc tctgggccct
     4201 cgcggtcatc gcgtacagga acgtctggcg gaacggcagg cgcacggccc tctgcgtcgt
     4261 cgccgtcgcc atagccgtct tcttcaacat cttcatgcgg tcctggatag agggcatgat
     4321 ggacggcatc gaggaggcgg tgcggacctt cgagacggga cacgtgaacg ccttcaccga
     4381 gcgcttcgag gccgagcggg aatacctccc cgtgcagtat cccctggacg agggcaggag
     4441 ctcggacgaa ctcgtggccg agatcgaggc cctgcccggc gtcgaggccg ccctgcccag
     4501 gatctccgcc tacgcgacgc tcttcgacag cacggtgaag cacgcggtcc tctggggcgt
     4561 cgatcccgcc agggagacga aggtcaaccg cctcaacctg acggaccgcg acgaggggct
     4621 cgtcgagggc cgcttccccg ccctgggcgc gaacgagtgc gccatcggcg cggagacggc
     4681 gagaaagacc ggcctccgga tcggcgacaa gctgcccctg aagaccgtct ccgcccagtt
     4741 ctccgacaag tactggagcc cgacggtcgt cggcgtcttc gagttcgact acgcgaagta
     4801 cgacgacggc gccgtcatcg tcccgatcga ccggctccgc cgggtcctcg ccctgggcga
     4861 ggccacccag gggatcttcg tctacgccag ggacgcggag gactcgcccg cgatcaaggc
     4921 gggagtggag cggatcctgg ggccgggcca cgtggtccgc caatggaccg acaactactg
     4981 ggtggcggtc atgcggcaga gctcctacat ctacgccatc gtcttcgccg tcttccaggt
     5041 cgtcgcgagc ttcctcatca tcaacaccgt cctcatggtg atccacgagc gcatcaagga
     5101 aatcgggatg atgggcgccc tcggcatgac gaggagggag atagtcctcg tcttcttctt
     5161 cgagtcggtg gtcctgagcg tcctcggctc gctggcgggc tgcgccctcg gcgccgcggc
     5221 gacggctatc ggctcgaact accccatcga catggagctg ttcacgggcg gcgggatgaa
     5281 ggagatgccg atctccggca ccctcttcct cgccttctcg cccggggcga tagtgggcgg
     5341 cttcggcttc ggcgtcctcg tctccgcggc ctgcaccctc ctgccctcgc tcaagagcgc
     5401 cttcatcgag cccgtcgagg ccctgcgccg ctagccgcgg cgccgcgaaa ggagagcgat
     5461 atgagaagca ttgcgatcga agccgcggag accggcgcgc gcgcgacggc gcctgcggct
     5521 gctgctcgga ctgcgcgtat ggcgcgaacg acggggactg cgcggacgcc gcgtcgcgcg
     5581 gccttcctcg ccttggccct gatgtccgcg ggagcctttc ctgcccccgt cttcgggcag
     5641 gcggcaggcg gccagccgag cgcggcggcc ctcctggagc gcgtcgacgc caacgaggtg
     5701 tactcctcca tcgcctacga gggcgagatg gccatcgagt accagggcaa gcgcttcgtg
     5761 aagcgcttca aggccttcgc gcgcgggggc tcggagagct tcatcgagtt cacgaacccc
     5821 gaggacgcag gcacgaaata cctgaagcgg gaggggcggc tctacgtcta ctctcccgac
     5881 gcggaggagg tcatgctgat ctccggccac ctcctcaagg aatccatgat gggctcggac
     5941 ctctcctacg aggacaccat cgagaacgag aagctctccg tccgctacga gccgagcctc
     6001 gccggccagg aggaggcatc cggccgcctg gcctacgtcc tcgacctccg cgcgaggcgg
     6061 agcggggaga gctatccgcg gcagaagctc tggatagacg cccagaccgg cgatctcctc
     6121 cgcgccgagt actacgccct gtcgggcgcc aagctgaagg agtatgcgct cctgcgcgcc
     6181 gagacgatcg gcgggcggcg cttccccgtg gagtcggaga tgcgggacct cctgcgcaag
     6241 ggctcgagga cggtcttccg gatgagcgcc gtgaggctcg acgcgcccat ccccgacgag
     6301 accttcagcg tgaggaacct ggaacgatga gaaggcagcc acgggccgcg gcgctcgccc
     6361 tcgccgccct cctcgcctcg ctcgccgcgg ccctgccagc agccgcggaa gggcccgcca
     6421 tatccggatc gatcgagacg agcgcggcgg cgagcctggg cgcgggcgcc tcccccgcct
     6481 tctccctcgg cttcgaggag tacgcaaacc tccgcctgaa ggctaaggct ggggagcgcg
     6541 gaaccgtcca cgcggcgctc aacctggtcg cagcctcggg gagcctggct ccgccggagg
     6601 ccgtcctagc ggcggcgccc tacgcgacgc ccttcgtctc cggcgggggc tacgccgccg
     6661 ccctggagct cgagcgcctc tactgcagga tcacgggcga gaccatggac ctggaggccg
     6721 gactcctgcg cctggccttc ggctacggcc aggccttcag cccgatggac ttcctctccg
     6781 cccgcaaccc cctcctgccg gaagcccgcc cccggggcgt cctcggcgtg gcggcgagcg
     6841 cctatccctc ggacctcgcg aggatcgaag ccttcgccgc cgcgggacgc gaccccttcg
     6901 agacctcggg agagggagcc ttggtaggcg cccttggcga actccacgga aggagcgcaa
     6961 gcctcgaaac cctctacgcc tacgaggcgc cgtccgaggc ctacggccgg ggggtccatc
     7021 gcgcgggcct ctccctcaag ctcgaggccg gagcaggcat agtcctcgac gcgctctaca
     7081 gccacgacgg cggggaaagg agcggcctcg gagggctcga gggaagcctc ggcctcgact
     7141 actcgctcgg aggcggcgac ctctatctcc tctgccagta cctctaccgt ggcccaggct
     7201 ccgtcggcga cctcacggga gggaactacc tctacgccgc cgcctcctgg cgcgtcgacg
     7261 actaccggag cattagcctc gcttccaccg cgaacctcga cgacctctcc gccctgcgct
     7321 tcctctccta cgagacggag cccttccagg gcttagccgt cctgt
//
LOCUS       DAOOSJ010000133         7331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6317_length_7331_cov_5.372045, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000133 DAOOSJ010000000
VERSION     DAOOSJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6317_length_7331_cov_5.372045"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLG14_07610"
     CDS             complement(<1..784)
                     /locus_tag="PLG14_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOX49041.1"
                     /translation="MRPSMFSVKPGAPLPLGARIVAEGVNFAVFSRHAEAMILSIYAT
                     PQDGEPVLEYRLDPRLNRTGDIWHVLVQGLKAGALYLWRADGPYLPRQGHRFNVHKAL
                     LDPYARALTGNFVWSLEAARAYDPESPELDLSFSDRDDAALMPKCVVVSDEFDWRGDR
                     PLNYPLRHCVIYEAHVRGLSLHPSSGVEHPGTFRGVVETIPRLKELGVTSLELLPVQE
                     FDELENPRRNPLTGEPLLNYWGYSTIAFFAPKARYSASGALGE"
     gene            complement(1102..2340)
                     /gene="fabF"
                     /locus_tag="PLG14_07615"
     CDS             complement(1102..2340)
                     /gene="fabF"
                     /locus_tag="PLG14_07615"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOX49042.1"
                     /translation="MARRRVVVTGLGVVSPVGNDVPGFWASLKAGKSGIGPITLVKAD
                     DLASRVAGEVKAFDPGLRMDAKEAKKMDRFSQFAVYAGLEALADAGLSKEGLDSERTG
                     VCLGTGQGGSSSIEEGVARLYERGPGRVPPLTAIKALANLGAANLAIYMNATGPSQCL
                     VTACAAGTDAIGTAYRWIADDHADLVFAGGSEASISRLGISSFAMIQALSTRNDEPEK
                     ASRPFDKDRDGFVIAEGAGVLVLEEYEAAKARGARIYCEVAGFANTCDAHHLTAPDPE
                     GKGVARAMLLAIKDAGLKPEDIDYVSAHGTSTPMNDPIETKALKHAFGAHAYKLKISS
                     IKSMIGHCMGAAGALESVATVKAIQEGFVPPTINLDNPDPECDLDYVPNKGVSMRVRA
                     AVKNSMGFGGQNAVVIFKKI"
     gene            complement(2342..3088)
                     /gene="fabG"
                     /locus_tag="PLG14_07620"
     CDS             complement(2342..3088)
                     /gene="fabG"
                     /locus_tag="PLG14_07620"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOX49043.1"
                     /translation="MDGMLIGKTCVVTGGSKGIGRAMVEAFAREGGRVYYLSRSEAED
                     PAALAAAAAQGGSVAWRKCDVAVEAEVDAVLEAVVKEAGSLDVVVNNAGVTRDGLVFR
                     MSLEDWETVLRTNLTSAFLVSRTAARHMIKKRAGSIINVSSVVGIMGNGGQTNYAASK
                     AGIIGFTKSLAREVASRGVRVNALAPGFIETAMTDKIPEEMKEKMKATIPLGRHGAPE
                     EVAKAALFLASDLASYVTGEVLKVDGGMGM"
     gene            complement(3194..4114)
                     /locus_tag="PLG14_07625"
     CDS             complement(3194..4114)
                     /locus_tag="PLG14_07625"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOX49044.1"
                     /translation="MKTCFLFPGQGAQYPGMGRDFYDTSKAVRELFELASDSAKMDLK
                     ALVFQGSEDDLKQTRNTQIAVALVGSAAALCAREHGIQPQGVAGFSVGEWPALAEAGV
                     VSHADMFRLVGERGRLMDEAGKRSGGSTMSAVLFMSPEAIEKTISDAGLKQVWIANYN
                     SPTQSVISGTEADISAAELALKAAGAKRVVRLKVSGAFHSPIMAYARDGFAELVSEVS
                     FADPKLELFSNVTGKLLSKGAEAKELAVRQIVSPVRWIEEEKSIIAAGYGQAVETGPG
                     LVLGGLWKAVAGDLPCHPCGTLDQVASISL"
     gene            4311..4625
                     /locus_tag="PLG14_07630"
     CDS             4311..4625
                     /locus_tag="PLG14_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOX49045.1"
                     /translation="MATESTERIEKVLKAIPKGRVLSYGEVAALAGVPNGARLVVRVL
                     HSRAEKSGLPWHRVLRKDGSIALPAGEGFDLQKALLEGEGVKVSPAGKVELGRYGWKG
                     PA"
     gene            complement(4771..5715)
                     /locus_tag="PLG14_07635"
     CDS             complement(4771..5715)
                     /locus_tag="PLG14_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOX49046.1"
                     /translation="MILPEARVFELSDSIIEDFIFAMEDQDGSWLVDLETGELIQLEG
                     EEAEGAAEEGGEERDLSDGERYAAPPEWSSREGFKLMEGFLGTVRQPSARNDLGAALS
                     RGKGVFKAFKEALAAHPELDKAFRDYKAREMRRSIAAWHDEQRELRGLARLGSEPEDT
                     GDLVESDLGISLGGKDDLLRLALPLLAEAELEALERLPEAVAAREAELLRARLATGEW
                     IGAWADNGEGGAIAVAAAERERSAGRAFGRLSFIFVQKPFRRAGLGSALVGALAEAFR
                     AEGTGVLVLDAPLAPADFGRALESTGFKPYGLRSISLG"
     gene            5901..6818
                     /locus_tag="PLG14_07640"
     CDS             5901..6818
                     /locus_tag="PLG14_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOX49047.1"
                     /translation="MEANLHLHSRYSDGSLWPAEMALRARETGLALAALTDHDSLGGV
                     PEFLAAAKAAGLEALAGCEMDCSLKDLGYRSEILAYFPEAKGEGDYPATAAFLRGVAR
                     AREKRLRVFVERSRALFRRSDLSFEELLARKLGASPPADLRRGELSFSKVDLFEYLKD
                     KGLVEKDLGYREFRKAYFDSELIKDGRFRRADAAEVAAPVLGDGGILVLPHPGHQFGD
                     DAGRMRAQKAELVLLLSRFRELGVSGVELYWYRNAHTQAINELVREAAEPLGYFFTYG
                     SDCHGPGSGKHTLGQFSGDFRGFPRPPRS"
     gene            6832..>7331
                     /gene="thyX"
                     /locus_tag="PLG14_07645"
     CDS             6832..>7331
                     /gene="thyX"
                     /locus_tag="PLG14_07645"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOX49048.1"
                     /translation="MAHCVVPAAEEILDRAFPVLDKGFVRLVDYLGGDARVVQAARVS
                     YGEGTKSFREDSGLIDYLLRHEHTSPFEQVVLTFHVKLPIFVARQWIRHRTARVNEIS
                     GRYSVMKDDFYVPAPGDLARQSADNKQGREDEALEAGEAAAVIARLEAGQKAAYAEYS
                     GLVESGL"
BASE COUNT         1070 a         2594 c         2559 g         1108 t
ORIGIN      
        1 gctcgcccag ggcgcccgac gcggaatacc tcgccttggg ggcgaagaag gcgatggtcg
       61 agtagcccca gtagttcagc aggggctccc cggtgagcgg gttccgcctc gggttctcga
      121 gctcgtcgaa ctcctggacg ggcaggagct cgaggctcgt gacgccgagc tccttcagtc
      181 gcggtatggt ctcgaccacg ccgcggaatg tcccggggtg ctcgacgccc gagctcgggt
      241 ggagggagag ccccctgacg tgggcctcgt agatcacgca gtgccgcagg gggtagttga
      301 gggggcggtc gccccgccag tcgaactcgt cggagacgac gacgcacttg ggcatcaggg
      361 ccgcgtcgtc cctgtcggag aaggagaggt cgagctcggg cgactcgggg tcgtaggcgc
      421 gggccgcctc caggctccac acgaaattgc ccgtgagggc cctcgcgtag gggtccagga
      481 gggccttgtg gacgttgaag cggtgcccct gccggggcag gtagggcccg tcggcccgcc
      541 agaggtagag ggcgccggcc ttgaggccct ggaccagcac gtgccagatg tcgcccgtcc
      601 tgttgagccg cgggtccagc cggtactcca ggaccggctc gccgtcctgg ggcgtcgcgt
      661 aaattgagag gatcatcgcc tcggcgtggc gcgagaagac ggcgaagttg acgccctcgg
      721 ccacgatgcg ggcgcccagg ggcaggggcg cgccgggctt cacggagaac atggagggcc
      781 gcacggacta agtatacagc gaagggcccc ttcgcgcgag ggcgaggacg agagcggcgc
      841 gccccttcgg cactgagcgc ccccgggtag ctctattgtc aatcacgtaa gaaaggaaaa
      901 gacggaaaga gatttccacg gattggcgcc gaaaggaaaa atattcggcg caggtccttt
      961 ccgtgcgaac cagcgctacc tctctccctc tcgtttctcc ttccgtgcaa atccgtggac
     1021 tctctccccc tcccgctcgg ctcgccacga aaaggggcgg ccctttcgga ccgccccctc
     1081 aagatccagc cagcggcggg cctagatttt cttgaaaatg acaaccgcgt tctgcccgcc
     1141 gaagcccatg gagttcttga ccgccgcccg cacccgcatc gagacgccct tgttggggac
     1201 gtagtccagg tcgcactcgg gatcgggatt atccaggttg atcgtcgggg gcacgaagcc
     1261 ctcctggatg gccttcacgg tcgcgacgga ctccagggcg ccggcggcgc ccatgcagtg
     1321 gccgatcatc gacttgatgg aggagatctt gagcttgtag gcgtgggcgc cgaaggcgtg
     1381 cttcagggcc ttggtctcga tggggtcgtt catcggggtc gaggtgccgt gggcggagac
     1441 gtagtcgatg tcctcgggct tgaggcccgc gtccttgatc gcgaggagca tcgcgcgcgc
     1501 cacgcccttg ccctcgggat cgggggcggt gaggtggtgg gcgtcgcagg tgttcgcgaa
     1561 gcccgcgacc tcgcagtaga tcctcgcgcc gcgggccttc gcggcctcgt actcctccag
     1621 gaccaggacg cccgcgccct cggcgatcac gaagccgtcg cggtccttgt cgaaggggcg
     1681 gctggccttc tccggctcgt cgttccgggt ggagagggcc tggatcatcg cgaagctgga
     1741 gatgcccagg cgggagatgc tcgcctcgga gccgcccgcg aagaccaggt ccgcgtggtc
     1801 gtcggctatc cagcggtagg cggtgccgat cgcgtccgtg cccgcggcgc aggcggtgac
     1861 caggcactgg ctcggacccg tcgcgttcat gtagatcgcc aggttggccg cgcccaggtt
     1921 ggccagggcc ttgatggctg tcagcggggg cacgcgcccg ggcccgcgct cgtagagccg
     1981 ggcgacgccc tcctcgatgc tggaggagcc gccctggccg gtgcccaggc agacgcccgt
     2041 ccgctcggag tccaggccct ccttggagag gcccgcgtcg gccagggcct ccaggcccgc
     2101 gtagaccgcg aactgcgaga agcggtccat cttcttcgct tccttcgcgt ccatgcggag
     2161 gcccgggtcg aaggccttca cctcgcccgc gacgcgggag gccaggtcgt cggccttgac
     2221 gagggtgatg ggcccgatgc ccgacttgcc cgccttgagc gaggcccaga acccgggcac
     2281 gtcgttgccc accgggctca cgacgccgag gccggtgacg accactcttc ttcttgccat
     2341 gctacatccc cattccgccg tcgactttga ggacctcgcc ggtcacgtag gaggcgaggt
     2401 cggaggcgag gaagagggcg gccttggcca cctcctcggg ggcgccgtgg cggccgaggg
     2461 ggatcgtggc cttcatcttc tccttcatct cctcgggtat cttatccgtc atcgcggtct
     2521 cgataaagcc cggcgcgagg gcgttgaccc tgacgccgcg gctcgcgacc tcgcgggcca
     2581 ggctcttcgt gaagccgatg atccccgcct tggaggcggc gtagttcgtc tggccgccgt
     2641 tgcccatgat gccgacgacg ctggagacgt tgatgatgga gcctgcgcgc ttcttgatca
     2701 tgtggcgggc ggccgtcctc gagacgagga aggcgctcgt gaggttcgtg cggagcacgg
     2761 tctcccagtc ctccaggctc atccggaaga cgaggccgtc gcgggtgacg cccgcgttgt
     2821 tgacgacgac gtccagggag cccgcctcct tgacgacggc ctccaggacg gcgtcgacct
     2881 cggcctccac ggcgacgtcg cacttcctcc aggcgaccga gccgccctgg gcggccgcgg
     2941 cggcgagagc ggccggatcc tcggcctcgc tccgggagag gtagtagacg cggccgccct
     3001 cgcgggcgaa ggcctcgacc atggcccggc cgatgccctt cgagcctccg gtcacgacgc
     3061 aggtcttgcc gatcagcatt ccatccatga tgtttccttt agacagcgcg ccgggcccgc
     3121 ggttgcggcc cggcgccgat gcggatgatt cccggcccgg cccgggcccg cggggcccgg
     3181 ccgggaggga agcctagagg gaaatggagg ccacctggtc cagggttccg caggggtggc
     3241 aggggaggtc gcccgcgacg gccttccaga gcccgcccag gaccaggccg gggcccgtct
     3301 ccacggcctg gccgtagcct gcggcgatga tggacttctc ctcctcgatc cagcgcacgg
     3361 ggctcacgat ctgcctgacc gcgagctcct tcgcctcggc gcccttcgaa aggagcttcc
     3421 ccgtcacgtt ggagaagagc tccagcttgg gatcggcgaa gctcacctcg gagacgagct
     3481 ccgcgaagcc gtcgcgggcg taggccatga tgggagagtg gaaggcgccg gagaccttga
     3541 ggcggacgac gcgcttcgcg cccgcggcct tgagggccag ctccgcggcg gagatgtcgg
     3601 cttccgtgcc ggagatgacc gactgggtcg ggctgttgta gttcgctatc cagacctgct
     3661 tgaggccggc gtcggagatg gtcttctcga tcgcctcggg ggacatgaag aggacggcgc
     3721 tcatcgtcga gccgccggag cgcttgccgg cctcgtccat gaggcggccg cgctctccca
     3781 cgaggcggaa catgtccgcg tgggagacga cgccggcctc ggccagggcg ggccactcgc
     3841 cgacggagaa gccggcgacg ccctgcggct ggatgccgtg ctcgcgggcg cagagggcgg
     3901 ccgcggagcc gaccagggcc acggctatct gggtgttgcg ggtctgcttg aggtcgtctt
     3961 cggagccctg gaagacgagg gccttgaggt ccatcttcgc ggagtcggag gcgagctcga
     4021 agagctcgcg gacggccttg ctcgtgtcgt agaaatctcg tcccatgccg ggatactggg
     4081 cgccctggcc cgggaacagg aaacaggtct tcatgaagga cctcctcgag tagcggcgtc
     4141 cggattccct cgcaggttcc ggcttcctca atcatttaca aattcggcaa aaatgtaaag
     4201 atggccgccc cggaatgccg acctggaagg ccgacccggt tgccgccctg gaaagacccg
     4261 cgcgcggccg cggccggatc gggctatact gggaccaaca aggaggcggc atggccaccg
     4321 agtcgaccga gcggatcgag aaggtcctga aagccatccc caaggggcgg gttctctcct
     4381 acggcgaagt cgcggccctc gccggcgtcc cgaacggcgc ccgcctcgtc gtcagggtcc
     4441 tccactcccg cgcggagaag tccggcctcc cctggcaccg cgtcctcagg aaggacggct
     4501 ccatagccct ccccgccggc gagggcttcg acctgcagaa ggccctcctc gagggggaag
     4561 gcgtgaaggt ctcccccgcc ggcaaggtcg agctcggccg ctacggctgg aagggtcccg
     4621 cctagacggg cccgcgcgcg cgggcggcgc ggaaagcgcc cgtggactcg aggacgcccg
     4681 cgccctcgcc cgccctagcc aagggaaatg gagcgcaggc cgtagggctt gaagcccgtg
     4741 gactcgaggg cgcccgcgcc ctcgcctctc ctagccaagg gaaatggagc gcaggccgta
     4801 gggcttgaag cccgtggact cgagggcgcg gccgaagtcg gcgggggcca ggggagcgtc
     4861 gagcacgagg acgcccgtcc cctcggcccg aaaggcctcg gccagggccc cgaccagggc
     4921 gctgcccagg ccggccctgc ggaagggctt ctgcacgaag atgaaggaaa ggcgcccgaa
     4981 ggcccgcccg gcgctccgct cccgctccgc cgcggccacg gcaatcgccc cgccctcgcc
     5041 gttgtcggcc caggccccga tccactcgcc cgtcgcgagt cgcgccctca ggagctcggc
     5101 ctcccgggcg gccaccgcct cgggcaggcg ctccagggcc tcgagctcgg cctcggccag
     5161 gaggggcagg gccaggcgga ggaggtcgtc cttcccgccc aggctgatgc ccaggtcgct
     5221 ctccacgagg tcgcccgtgt cctcgggctc ggagcccagg cgggccaggc cccggagctc
     5281 gcgctgctcg tcgtgccagg cggcgatgct gcgccgcatc tcgcgggcct tatagtcgcg
     5341 aaaggccttg tccagctcgg ggtgggccgc cagggcctcc ttgaaggcct tgaagacccc
     5401 cttgccccgg gagagggccg cccccaggtc gttgcgggcg ctcggctgcc gcaccgtgcc
     5461 cagaaagccc tccatgagct tgaagccctc ccgcgaggac cattccggag gcgcggcgta
     5521 acgctcgccg tcggagaggt cgcgctcctc cccgccctcc tcggcggcgc cctcggcctc
     5581 ctcgccctcg agctggatga gttcgcccgt ctccaggtcg acgagccagg agccgtcctg
     5641 gtcctccatc gcgaagatga aatcctcgat gatcgaatcg ctgagctcga acacgcgggc
     5701 ctccggaagg atcatagtgg aggcgccttg gccgatggtc aaggccgatt cgccggccgc
     5761 ctcccgcggc gacgcggtcc cccacggggc gagccagtcc gcgcggggcg cctgtccgcg
     5821 ccaggcgccc ttccgcgccg gccacggacg ccgcggccct tccccgccgg gcttccggcc
     5881 gctatactcg gcccagagcc atggaagcga acctgcacct ccactcgcgc tactcggacg
     5941 gcagcctctg gcccgccgag atggccctac gggcccggga gaccggcctt gccctcgcgg
     6001 ccctgaccga ccacgacagc ctgggcgggg tgcccgagtt cctcgccgcg gccaaggccg
     6061 ccggcctgga ggcgctcgcg ggctgcgaga tggactgctc cctcaaggac ctgggctacc
     6121 ggagcgagat cctggcctac ttcccggagg ccaagggcga gggggactat ccggccacgg
     6181 ccgccttcct ccgaggcgtc gcccgggcgc gcgagaagcg gctccgcgtc ttcgtcgagc
     6241 gctcccgcgc cctcttccgc cgctccgacc tctccttcga ggagctcctc gcgcggaagc
     6301 taggcgcctc ccctccggcg gacttgcggc gcggcgagct ctccttcagc aaggtcgacc
     6361 tcttcgagta cctgaaggac aagggcctcg tggagaagga cctgggctac cgcgagttcc
     6421 gcaaggccta cttcgactcc gagctcatca aggacggccg cttccgccgg gccgacgccg
     6481 cggaggtcgc ggctcccgtc ctcggggacg gaggcatcct cgtccttccc cacccgggac
     6541 accagttcgg cgacgacgcc gggaggatgc gcgcccagaa ggcggagctc gtcctcctcc
     6601 tgtcgcgatt ccgggagctc ggcgtctccg gcgtcgagct ctactggtac cgcaacgccc
     6661 atacccaggc catcaacgag ctcgtgcggg aggcagccga gcccctcggc tacttcttca
     6721 cctacggctc ggactgccac ggccccggct cgggcaagca caccctcggc cagttctcgg
     6781 gcgacttccg cggcttcccc cggccgcccc gcagctaagg agagttccag catggcacat
     6841 tgcgtcgtcc ccgccgccga ggagatcctc gaccgggcct tccccgtcct cgacaagggc
     6901 ttcgtccgcc tggtcgacta cctgggcggc gacgcccgcg tcgtccaggc cgcccgcgtc
     6961 tcctacggcg agggcacgaa gagcttccgc gaggactcgg gcctcatcga ctacctcctc
     7021 cgccacgagc acacctcgcc cttcgagcag gtcgtcctca ccttccacgt gaagctcccg
     7081 atcttcgtcg cgcggcagtg gatcaggcac cggaccgccc gcgtcaacga gatctccggc
     7141 cgctactcgg tcatgaagga cgacttctac gtcccggccc ccggcgacct cgccaggcag
     7201 agcgccgaca acaagcaggg ccgcgaggac gaggccctgg aggccgggga ggcggccgcc
     7261 gtcatcgccc gcctggaggc ggggcagaag gccgcctacg ccgagtactc gggcctcgtc
     7321 gagtcgggcc t
//
LOCUS       DAOOSJ010000134         7266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6385_length_7266_cov_5.828734, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000134 DAOOSJ010000000
VERSION     DAOOSJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6385_length_7266_cov_5.828734"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1746)
                     /locus_tag="PLG14_07650"
     CDS             complement(205..1746)
                     /locus_tag="PLG14_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX49049.1"
                     /translation="MQCAAPEPPLRGFPAGEAGPVNPVRVIFAVGDEAERAAFSSLAG
                     PETCCVVHTPGDIAEVEKLFSADAADVIVTDFSFHSGAFADWLTFWPLPAVLLVDAGD
                     DPERIERTIRDEASLFVERDPEGRYLERLPILVRKVLNIRESINRQNAHLKMTEHQYL
                     NLLQAVPDIVYILDGDGRFMYLNDSVRNLGFEPAKLIGKHFSEIVHPTDVPKVSRHEV
                     LRSFKGVATGADNAPKLFDERRAGERMTKNLELRLRRNVGPEGFLYASVNAYGEVNCT
                     GWKLPEYENLELGTVGIIRDITVRKEHEKELEDALAAKEILLKEIHHRVKNNLQVVSS
                     LLNLQENVVEDESARKVFLECQTQVQSMAMVHEVLYRSDNFEGVEMQKYFQSLIDYLS
                     GIYDGPYRGVAFDIEADAVTLDLDEAIPVALIVNELVSNSFKHAFPDGRAGKVRVAMA
                     ARDSGWELAVSDDGVGFAATRNPAREHGLGTELIHALTAQLHGELEQDSSAAGSTTRI
                     RFPRP"
     gene            1884..3209
                     /locus_tag="PLG14_07655"
     CDS             1884..3209
                     /locus_tag="PLG14_07655"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOX49050.1"
                     /translation="MPSDLDFSVTALGERRVPSPIGLSTTQGDRIANYVGDDELVRYG
                     AEARPGAQEPLERRALLEKAGPRERIYFGPSHVHAAIVTCGGLCPGLNDVIRAITRCL
                     WYRYGVRRISGIRYGYKGFLPEYEAAVRTLDPEVVDDIHKMGGTILGSSRGGGERTGE
                     IVDAIERLNVNMLFTIGGDGTQKGALAIAEEIERRRLKIAVVGIPKTIDNDLLFVDRS
                     FGFETAVAGAAEAVAGAHAEAHSSMNGIGLVKLMGRDSGFIAVHTVLAVHEANFVLVP
                     EVPFDLEGPAGLLARLEERLARRKHAVVVVAEGAGQEHLAATEGRDASGNRRYSDIGL
                     FLKERIQAHFEAKGLEVNLKYIDPSYLVRSAPASPTDSVYCERLGNNAAHAAMAGKTK
                     CLIGMVNNEFVHIPMAVAVSSRRRVDPEGALWRDAVDATQQPLSLKNEA"
     gene            3214..4491
                     /locus_tag="PLG14_07660"
     CDS             3214..4491
                     /locus_tag="PLG14_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX49051.1"
                     /translation="MAKKTALVIDESDLFREYLRSRLAEHGIEVEVAVNGLDGIVKMR
                     NSLPDVVLLDYHLTRKTCKELLEEKNRNPNTAAIPVILTAQKIDKNRLLELVPFNIKK
                     VFMKPIKMDSLYGTLSELLGVRFAMDETPCIIEAHVNDNIVFIEIAKGLNREKIDLLR
                     FKIAELLELYAIRNPRILIMITDMELSFVDGSNLELLLSTVLEHSRTKAKHVKVLTNE
                     AFVREFARGRPEFSEIEVLSNLQYAMDGLIADYDRDGDGGESKAEVISERILSAQSLR
                     SGGESIEMRFDAEGRKAYSLEAIKEAGHGLSIAVVDDDFVIQELVKTTFAAISADVTP
                     YSNGREFLDALKGKSFDLVFLDILMPELDGFGVLQRLHAEDIDVPVIVLSAVSQREAV
                     VRAFQAGVKSYLIKPLKPDQILKKTLEILKANF"
     gene            4502..>7266
                     /locus_tag="PLG14_07665"
     CDS             4502..>7266
                     /locus_tag="PLG14_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX49052.1"
                     /translation="MAFPEAVFRASFEQSSQPLAVVDGAGLLAIWNGAFEAFFSGLAG
                     VGPARLKTSLLDFLEERESLRFDYYAAELLLGGRDSALVETPVRAADGTRHWLRVGLS
                     LLEMAPKPGAEARPERFLLCSLEDVTDRVAREQRLRDEKEEAEKATHTKSQFLANMSH
                     EIRTPIQTILGVVELLQETGLDGEQAEYASQVRFSADILLGLINDILDFSKIEADKLD
                     LETIDFDLRACVQQSVDLLVMEAHRKGLEVIVDVDEELPGLVRGDPARIRQIIVNLFK
                     NAVKFTRDGCISLRLRKTAGSRGPFVRFEVADTGIGIPEDVRLRLFTPFFQADFAAAR
                     KAGGTGLGLAISRRLSELMGGSIGVLPNEPRGSVFWFELPLASPEFSAPPAAPPAAPD
                     ARLLIVDDYSDAREFAARTASRAGYRTATAASGEEALAALRGAAKAQDPFALCLIDQN
                     MPVMDGWRLASEITGDISINGARLILMVPEGTMGGDAKMKLLRWFNAYVPKPLRPADL
                     LETLRRALSSDVDLESADAAEEAGAAGAAGAPEDGQAEPEPSFEASVLLAEDHEVNRE
                     LFTLILGRLGCRVVAARDGVEAVEIGSSRGFDIVLMDIFMPRMNGYEASLSLRERGYR
                     GPIIAVTASALKGEREKCVEAGMDDILVKPFKKHDLATVLSAWLPSRLGGSRAEAEGQ
                     AAAPFRSAAASPSSALAAPSSPGAAAKPAAGTAAASVANAGAAAKPAAPPRPAAEGIP
                     WAAEEAAPSDRTSGPSLLAEARRRAEGLKAGAAQAAAAPGAAPAGTPAAPRARGQARQ
                     DQAAQGSGAALPDAGVFDWEGVIDTFLGQKETVVNLLRKFADMASRQVEELGAALEAK
                     DFPRFREVAHSLKGASWNLSARALGDAALAAEDAGKRGDAEAAKAARAALGPALGAFL
                     AAIKPYT"
BASE COUNT         1145 a         2635 c         2448 g         1038 t
ORIGIN      
        1 ttcgtcgcgg aggcgcacaa ccgcttcgcg gccctctaca acaaggggga ctaccaaggc
       61 gcgcggctcg cggtctccga ggccctggcc gccctcccgg gcgacccgac cctcaaggcc
      121 gacctggccc tcgcggagaa ggccctgggg cgctaggggc ggccgcggcg ggcgcgcgcc
      181 ttggccccgg accggagcgc ggcgctaggg gcgggggaag cggatgcggg tggtcgagcc
      241 cgcggccgag gagtcctgct cgagctcgcc gtggagctgg gcggtcagcg cgtggatgag
      301 ctcggtgccc agaccgtgct cgcgggccgg gttgcgcgtc gccgcgaagc ccacgccgtc
      361 gtcggagacg gcgagctccc agcccgagtc cctcgcggcc atcgcgaccc tcaccttgcc
      421 cgcccgcccg tcggggaagg cgtgcttgaa ggagttggac accagctcgt tgacgatgag
      481 ggcgacggga atggcctcgt ccaggtccag ggtgaccgcg tccgcctcga tgtcgaaggc
      541 cacgccgcgg tagggcccgt cgtagatgcc cgagaggtag tcgatgaggc tctggaagta
      601 cttctgcatc tccacgccct cgaagttgtc ggagcggtag aggacctcgt ggaccatcgc
      661 catcgactgg acctgggtct ggcactccag gaagaccttg cgcgccgact cgtcctccac
      721 gacgttctcc tggaggttga ggaggctgga gaccacctgc aggttgttct tgacgcggtg
      781 gtggatctcc ttgaggagga tctccttggc ggccagcgcg tcctcgagct ccttctcgtg
      841 ctccttgcgc acggtgatgt cgcggatgat gccgaccgtg cccagctcca ggttctcgta
      901 ctcgggcagc ttccagcccg tgcagttcac ctcgccgtag gcgttgacgg aggcgtagag
      961 gaagccctcg ggcccgacgt tccgcctgag gcggagctcc aggttcttcg tcatcctctc
     1021 cccggcgcgc cgctcgtcga agagcttggg cgcgttgtcc gcgccggtgg cgacgccctt
     1081 gaaggagcgc agcacctcgt ggcggctcac cttgggcacg tccgtggggt ggacgatctc
     1141 gctgaagtgc ttgccgatga gctttgccgg ctcgaagccc aggttcctga ccgagtcgtt
     1201 caggtacatg aagcgcccgt cgccgtccag gatgtacacg atgtccggca ccgcctgcag
     1261 caggttcagg tactggtgct cggtcatctt gaggtgggcg ttctggcggt tgatgctctc
     1321 gcggatattg agcaccttgc ggaccaggat agggagcctc tccaggtagc gcccctcggg
     1381 atcccgctcg acgaagaggc tcgcctcgtc ccggatcgtc cgctcgatgc gctcggggtc
     1441 gtcgcccgcg tccacgagga ggacggcggg gaggggccag aaggtcagcc agtcagcgaa
     1501 ggcgcccgag tggaaggaga agtccgtgac gatgacgtcc gccgcgtccg cggagaagag
     1561 cttctccacc tcggctatgt ccccgggggt gtggacgacg cagcaggtct ccgggcccgc
     1621 cagggaggag aaggccgccc gctcggcctc gtcgccgacg gcgaagatca cgcggacggg
     1681 attcaccgga ccggcctccc cggccgggaa gccccggagc ggaggctcgg gcgccgcgca
     1741 ctgcatcgca tcggctaata ataagtcggc gtttccggga tagtcaaggg aagcggcatt
     1801 gcctcctggc gcgggcggaa ctatactcaa gggcgccacg cccaggggcg gcgccagccc
     1861 cccccgcgtg ccagggggag aagatgccat ccgacctcga cttcagcgtg acggccctcg
     1921 gagagcggcg cgtcccctcg cccatcgggc tttccacgac gcagggcgac aggatcgcca
     1981 actacgtcgg cgacgacgag ctcgtgcgct acggcgcgga ggcgaggccg ggagcacagg
     2041 agcccctgga gcgccgcgcc ctcctggaga aggccggccc ccgcgagcgg atctacttcg
     2101 gcccgagcca cgtccacgcg gccatcgtca cctgcggcgg gctctgcccg ggcctcaacg
     2161 acgtcatcag ggccatcacc cgctgtctct ggtaccgcta cggcgtgcgc cggatctcgg
     2221 ggatccgcta cggctacaag ggcttcctcc ccgaatacga ggccgcggtc cggaccctcg
     2281 accccgaggt cgtcgacgac atccacaaga tgggcggcac gatcctgggc tcctcccgcg
     2341 gcggcggcga gcggacgggc gagatcgtgg acgcgatcga gcggctcaac gtcaacatgc
     2401 tcttcacgat cggcggcgac ggcacccaga agggcgccct ggccatagcg gaggagatcg
     2461 agcgccggcg gctcaagatc gccgtcgtcg gcatcccgaa gaccatcgac aacgacctcc
     2521 tcttcgtgga caggtccttc ggcttcgaga ccgccgtcgc gggcgccgcg gaggccgtcg
     2581 ccggcgccca cgcggaggcc cactcctcca tgaacgggat cggcctcgtg aagctcatgg
     2641 gccgcgactc gggcttcatc gcggtacaca ccgtcctcgc cgtccacgag gccaacttcg
     2701 tcctcgtgcc ggaggtgccc ttcgacctgg aggggccggc cgggctgctc gcccgcctcg
     2761 aggagcggct cgcgcggcgg aagcacgccg tcgtcgtcgt cgcggagggc gcgggacagg
     2821 agcacctggc cgcgaccgag ggccgcgacg cctccggcaa ccgtcgctac tcggacatag
     2881 gcctcttcct caaggagcgc atccaggccc acttcgaggc caagggcctc gaggtcaacc
     2941 tcaagtacat cgacccgagc tacctcgtgc gctcggcgcc ggcgagcccg acggactccg
     3001 tctactgcga gcgcctgggc aacaacgcgg cccacgccgc gatggccggc aagacgaagt
     3061 gcctcatcgg catggtgaac aacgagttcg tgcacatccc catggccgtc gcggtgagct
     3121 ccaggcgccg cgtcgacccc gagggagccc tctggcgcga cgcggtcgac gcgacgcagc
     3181 agcccctgag cttgaagaac gaggcgtaag gacatggcca aaaagaccgc gctcgtgatc
     3241 gacgaatcgg acctcttccg agagtacctc cgctcccggc tcgccgagca cgggatcgag
     3301 gtggaggtgg cggtgaacgg cctggacggc atcgtgaaga tgcgcaacag cctgccggac
     3361 gtcgtcctcc tcgactacca cctcacgagg aagacctgca aggagctcct cgaggagaag
     3421 aaccggaacc ccaacacggc ggccataccg gtcatcctca ccgcgcagaa gatcgacaag
     3481 aaccgcctcc tcgagctcgt ccccttcaac atcaagaagg tcttcatgaa gcccatcaag
     3541 atggacagcc tctacgggac gctctccgag ctcctgggcg tccgcttcgc gatggacgag
     3601 accccctgca tcatcgaggc ccacgtcaac gacaacatcg tcttcatcga gatcgcgaag
     3661 ggcctgaacc gcgagaagat cgacctcctc cgcttcaaga tcgcggagct cctggaactc
     3721 tacgcgatcc ggaacccgcg catcctcatc atgatcaccg acatggagct ctccttcgtc
     3781 gacggctcca acctcgagct cctcctctcc acggtgctgg agcactcccg cacgaaggcg
     3841 aagcacgtca aggtcctcac gaacgaggcc ttcgtccgcg agttcgcgcg gggccggcca
     3901 gaattctcgg agatcgaggt gctctcgaac ctccagtacg cgatggacgg cctcatcgcc
     3961 gactacgacc gggacggcga cggcggcgag agcaaggcgg aggtcatctc cgagcggata
     4021 ctctcggcgc agagcctccg gtctggcggc gagtccatcg agatgcgctt cgacgccgag
     4081 ggcaggaagg cctacagcct ggaggcgatc aaggaggcgg gccacggcct ctccatcgcc
     4141 gtcgtcgacg acgacttcgt gatccaggag ctcgtgaaga cgaccttcgc cgcgatcagc
     4201 gccgacgtga ccccctactc gaacggcagg gagttcctgg acgcgctcaa gggcaagagc
     4261 ttcgacctcg tcttcctcga catcctcatg cccgagctcg acggcttcgg ggtgctccag
     4321 cgcctgcacg cggaggacat cgacgtgccg gtcatcgtcc tctccgccgt ctcccagcgc
     4381 gaggccgtcg tccgcgcctt ccaggccggc gtcaagagct acctcatcaa gcccctcaag
     4441 cccgaccaga tactcaagaa gaccctggag atcctgaagg cgaatttcta gaggaggagc
     4501 cgtggccttc cccgaggcgg tattccgcgc gtcgttcgag cagtcctccc agcccctcgc
     4561 cgtcgtggac ggggcgggcc tcctcgcgat ctggaacggg gccttcgagg ccttcttctc
     4621 cggcctcgcc ggagtgggcc cggcccgcct caagacctcc ctcctcgact tcctggagga
     4681 gagggagagc ctccgcttcg actactacgc ggccgagctc ctcctcgggg gccgggactc
     4741 ggccctcgtc gagacgcccg tacgcgcggc cgacgggacg aggcactggc tcagggtcgg
     4801 cctctccctc ctcgagatgg ccccgaagcc cggcgccgag gcccggcccg agcgcttcct
     4861 cctctgctcc ctggaggacg tgaccgaccg cgtcgcgcgc gagcagcggc tccgcgacga
     4921 gaaggaggag gccgagaagg cgacgcacac caagagccag ttcctggcca acatgagcca
     4981 cgagatccgc acgcccatcc agaccatcct gggcgtggtc gagctcctcc aggagacggg
     5041 cctggacggc gagcaggcgg agtacgcgtc ccaggtgcgc ttctccgcgg acatcctcct
     5101 cggcctcatc aacgacatcc tggacttctc caagatcgag gcggacaagc tcgacctgga
     5161 gacgatcgac ttcgacctgc gcgcctgcgt ccagcagtcc gtcgacctcc tggtcatgga
     5221 ggcgcaccgg aagggcctgg aggtcatcgt cgacgtggac gaggagctgc ccggcctggt
     5281 gcgcggcgac cccgcgagga tccgccagat catcgtgaac ctcttcaaga acgccgtgaa
     5341 gttcacccgc gacggctgca tcagcctccg cctccggaag acggccgggt cccgggggcc
     5401 cttcgtccgc ttcgaggtgg ccgacacggg catcggcata ccggaggacg tgcgcctccg
     5461 cctcttcacg cccttcttcc aggccgactt cgccgcggcg aggaaggccg ggggcacggg
     5521 cctgggcctc gcgatctcgc gccgcctctc cgagctcatg ggcggctcca tcggcgtcct
     5581 cccgaacgag ccgcgcggct cggtcttctg gttcgagctg cccctcgcct cccccgagtt
     5641 ctccgccccc ccggccgccc cgcctgccgc cccggacgcg aggctcctca tcgtcgacga
     5701 ctactcggac gcccgcgagt tcgccgcgag gaccgcctcc cgcgcgggct acaggacggc
     5761 gacggccgcc tcgggagagg aggccctggc ggccctccgc ggggccgcga aggcccagga
     5821 tcccttcgcg ctctgcctca tcgaccagaa catgcccgtg atggacggct ggcgcctcgc
     5881 gagcgagatc acgggcgaca tctcgatcaa cggagcccgc ctcatcctca tggtgcccga
     5941 gggcacgatg ggcggcgacg cgaagatgaa gctcctgcgc tggttcaacg cctacgtgcc
     6001 caagcccctc aggcccgccg acctcctcga gaccctgagg cgggccctct cctccgacgt
     6061 cgacctcgag tcggccgacg ccgcggagga ggccggggct gccggagcgg ccggcgcgcc
     6121 cgaggacggg caggccgagc ccgagccgag cttcgaggcc tccgtcctcc tcgcggagga
     6181 ccacgaggtc aaccgcgagc tcttcaccct catcctgggg cggctcggct gccgcgtcgt
     6241 cgccgcccgg gacggcgtgg aggccgtgga gatcggctcc tcgcgcggct tcgacatcgt
     6301 cctcatggac atcttcatgc cgcggatgaa cggctacgag gcctccctct ccctgcgcga
     6361 gcgcggctac cgcggcccca tcatcgccgt gacggcgagc gccctcaagg gcgagcggga
     6421 gaagtgcgtc gaggcgggca tggacgacat cctcgtgaag cccttcaaga agcacgacct
     6481 ggccacggtc ctctcagcct ggctgccgtc gaggctcgga ggctcgcgcg ccgaggccga
     6541 gggccaggcg gccgcgccct tccgctccgc cgcggcgtcg ccctcctcgg ccctggcggc
     6601 gccctcctcg cccggcgcgg ccgcgaagcc cgccgcgggg acggccgccg cgagcgttgc
     6661 caacgccggc gcggccgcga agcccgccgc gccgccgcgc ccggccgccg aaggaatccc
     6721 ctgggcggcg gaggaagcgg cgccgtcgga ccggacttcc ggcccctccc tcctcgccga
     6781 ggcgaggagg cgcgcggaag gcctgaaggc tggcgcggcc caggccgccg ccgcgccagg
     6841 cgccgccccg gcgggtacgc ccgcggcgcc ccgggccagg ggccaggcac gacaggacca
     6901 ggccgcccaa ggctcgggcg cggcactccc cgatgccggc gtcttcgact gggagggcgt
     6961 catcgacacc ttcctgggcc agaaggagac cgtcgtcaac ctcttgcgga aattcgcgga
     7021 catggcgtcg cggcaggtcg aggagctcgg cgcggccttg gaggccaagg acttcccccg
     7081 cttcagggag gtcgcccact cgctgaaagg agcctcctgg aacctctccg cgagggccct
     7141 gggcgacgca gccctcgccg cggaggacgc gggcaagcgg ggcgacgccg aggcggccaa
     7201 ggccgccagg gccgccctcg gccccgccct cggagccttc ctcgcggcca tcaagcccta
     7261 caccga
//
LOCUS       DAOOSJ010000135         7190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6474_length_7190_cov_4.432516, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000135 DAOOSJ010000000
VERSION     DAOOSJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6474_length_7190_cov_4.432516"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..663)
                     /locus_tag="PLG14_07670"
     CDS             complement(103..663)
                     /locus_tag="PLG14_07670"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX49053.1"
                     /translation="MKIKYNAPATLTFTLVSASVLFLSGTLMPGLTQSWFSTPAPFRA
                     NQAQDYVKLFTHVLGHGSIEHFLSNFSFILLLGPILEATYGSLSITLMTAVTALVTGL
                     LNVLLFPNTILLGASGVVFMMILLASFTNFNKGEVPLTFILVMALFLGKEVWSAVANK
                     DNISQFAHVLGGLVGSFFGFLRPRKQ"
     gene            complement(708..1493)
                     /locus_tag="PLG14_07675"
     CDS             complement(708..1493)
                     /locus_tag="PLG14_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOX49054.1"
                     /translation="MTTRILSFNVNGVRACAGKGLFDWLAADSPDILCLQETKAHPEQ
                     LPKEFLAPSDGNGGAYRAYYASAKRRGYSGVAILSKEEPRTIGALGVPEFDEEGRTLV
                     ADFGGFVLFSCYFPNSQEAGARLDYKIRYCDAVLSACDRLVAAGRHVVVAGDYNIAHE
                     PIDLANPKANEGNPGYLPEERAWFDKWAGAGYADSFRRLCPEPGKYSWWTYRVPSARE
                     KNIGWRLDYHCVDEGLMPAVRAATIHPEVRGSDHCPVGIELEL"
     gene            complement(1518..2540)
                     /locus_tag="PLG14_07680"
     CDS             complement(1518..2540)
                     /locus_tag="PLG14_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49055.1"
                     /translation="MIARKRAAPAAVLLLLALFAPGRAAVGAEELRTIVAGTVSLSPE
                     ASEGTRLSIGYNDGFAILLSKDSPFIQGFEIELKSPQAALAFPGALAYELWRRVDPLP
                     DKNRYGYEGSRILTQALPARAGIVIQVPVRKDHSLKSGPYATLLPVVAEPKDFPLVFR
                     LFPVTKGLSPELEAAQFQVRVRPILTDEGALRLMLRYPEGAERAGVAVFVDDKRLPEG
                     RYQDGKELLPLKAGSHSLRVSSDCFRDESRSFQVEQGRILELAVELQDTMPLLVIEAP
                     DSAQLSLDGARIDFLAKPSFAVEPGEHNLSCRIGDYVVSRKFTAYRGKTYRLVLSVEL
                     QLQESP"
     gene            complement(2537..3499)
                     /locus_tag="PLG14_07685"
     CDS             complement(2537..3499)
                     /locus_tag="PLG14_07685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX49056.1"
                     /translation="MSLGPRRPERAGRGLLLALAFLLSSASALASEPPKAILAAAKAS
                     YPSLGSLGPDDPIYRQHQEQLAASYAALSSGGPAPELILYSYTARNVLDLLSIAARLN
                     LPYETLATLNGLDRARPFERGETILVPSAPGLFAPAKPRTDLDFLISYRGEAGGRSLS
                     VEGPRGALSLLFYRGERFNAEERALFLGLLFRLPLPAARLTSGFGLRTSPVSGRTAMH
                     SGIDLAAPSGTEVYAAREGRVSASGVDPVLGEFIVVDHEGGWQTVYGHLSTRYARLND
                     RVESSMMIGRVGSTGLSTGPHLHFEVRSRGEPRNPEPLIPKVKP"
     gene            complement(3496..3909)
                     /locus_tag="PLG14_07690"
     CDS             complement(3496..3909)
                     /locus_tag="PLG14_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49057.1"
                     /translation="MSRKAERAGEAASRALERGDRSARAAERLAHVLLAGLGIVVASA
                     AAVWPLWALATERRSLFNALMGGLALALLAFAAARRLGARRQGAGRRGAKPRGAAPGA
                     DPRGADLRGAKVRDAKLRDDQLRDAGLGESPAPRP"
     gene            complement(3906..4778)
                     /locus_tag="PLG14_07695"
     CDS             complement(3906..4778)
                     /locus_tag="PLG14_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49058.1"
                     /translation="MTSATTSLFVLRAGPEGPLVIHPFGPGSPLYAEAEAAPEPPRFE
                     GRFAAGPSPSSFYELRGALDAELEAGLRERALDSGFVLRVAAVSGAFLFVYLFLSIVV
                     RDPIPLVDELAGASLAALAARSVLRRRALSSPRTAELGAALRRALGEAYFTESRLVAL
                     AESWLEEAFPALGVAAADSLGAAAFAGGGKVADGAALEAWLAAEPPALSSDDFLELGF
                     LTELCGRELAKSGLLGPEPVAETEAAARRRLSRLDPRRRLSGRGLREAELSALFLKGR
                     AVLERGFPDAEAKP"
     gene            complement(4810..>7190)
                     /locus_tag="PLG14_07700"
     CDS             complement(4810..>7190)
                     /locus_tag="PLG14_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49059.1"
                     /translation="KEVERLIAEALKNGSFVLPPAVLAESALPQAAPRAAAAPAPKAQ
                     AKPVAVQAEPRAAPPTLAAPPSLEPEAAAEAEELEELEELEELGEAEEVAAIPAPAAA
                     KETGPGTEAEVEELEELEELEELEELEEGEAEAPAAAPSPAAAAAPSPAAAAAPSPAV
                     AAATPEPAVAIEIPEAAEPAEAAEAEELEELEELEELEELEELEEGEPEAPAAGAVEP
                     AVAPAPAGQAEPAAAPEAVEIAEAEELEELEELEALEEMEEAEEAAAEPVPQAAAGAA
                     FEQMPAFPFEAPPRVQPALKPAAEEEVHAPAAAEGGEPEELEELEACEELEPVEVESA
                     AEGGEAEELETLEPAESEFDYGALAVEEEVAFGDLRRAAARALELARDESEDIPLIPE
                     SSGLELADEADIADIIGFIDMEDTSRSAYLDFAGPAEGEVLGRAVPLADAEARKLAAL
                     EEALEEVPELAVLEEAEFAGEPAPPPEPETPVVFQPEPVIVPLEEMEFELLLGSLDLS
                     SLEQWDRGPAGADEIDATRSDYREEVAAAASAEAAGTPSPAVAAAVPSVPAAKAAPGL
                     EAAPAELEPVEAEPEELEPVEAELVEPEELEPVEAEPIEPIAHEPVAPAAVPPAAVAA
                     AAPGPVGSSPVAPAAEVPETAASPAAEAAPAELPLAESATAEESGEQAAELGASLGAF
                     RAYSPPSRARGPFMGAPVEGELPVVEEAEEGEAEGELLESPDFDFPATEDEDEVPADP
                     QGGPIVYRDGLYQLDAGYGTSLGGLDPSLRDLVDSVLGDGGQDESRRDPPSP"
BASE COUNT         1004 a         2370 c         2850 g          966 t
ORIGIN      
        1 gagcgcgggg ggccgctccg caaggcgaaa acgaagcgcc ggcggccgcg atcgccgccg
       61 ctctcgggcg gaaccgcggg tctccgcccg tctctctccc tgctattgct ttctcgggcg
      121 aaggaagccg aagaaggagc cgacgaggcc gcccaggacg tgggcgaact gggagatgtt
      181 gtccttgttc gcgaccgcgc tccagacttc cttgccgagg aagagggcca tcacgaggat
      241 gaaggtgagg ggcacctccc ccttgttgaa gttggtgaag gaggcgagga ggatcatcat
      301 gaagacgacg ccgctcgccc cgaggaggat ggtgttcggg aagaggagga cgttgaggag
      361 gccggtgacg agggcggtga ccgcggtcat gagcgtgatc gagagggagc cgtaggtcgc
      421 ctccaggatg ggcccgagga ggaggatgaa gctgaagttg gagaggaagt gctcgatgct
      481 cccgtggccg aggacgtggg tgaagagctt cacgtagtcc tgggcctggt tcgccctgaa
      541 gggggccggc gtcgagaacc aggactgggt gagccccggc atgagggtcc ccgagagaaa
      601 gaggacggaa gccgagacga gggtgaaggt gagggtggcg ggagcgttgt acttgatctt
      661 catgcgttcc tccgcgatcg ggccggggcg gctcgccccc cggcgggcta gagctcgagt
      721 tcgatgccca ccgggcagtg gtcggagccg cggacctcgg ggtggatggt cgcggcgcgg
      781 acggccggca tgaggccctc gtcgacgcaa tggtagtcca ggcgccagcc gatgttcttc
      841 tcccgagcgc tcggcacgcg gtaggtccac cagctgtact tgccgggctc ggggcagagg
      901 cgccggaagc tgtcggcgta gccagcgccc gcccacttgt cgaaccaggc ccgctcctcc
      961 ggaaggtagc cggggttccc ctcgttcgcc ttggggttcg cgaggtcgat gggctcgtgg
     1021 gcgatgttgt agtccccggc cacgacgacg tggcggcccg cggccacgag gcggtcgcag
     1081 gcggagagca cggcgtcgca gtagcggatc ttgtagtcga ggcgggcgcc ggcctcctgg
     1141 ctgttcggga agtagcagga gaaaaggacg aacccgccga agtccgccac gagggtcctg
     1201 ccctcctcgt cgaactcggg gacgcccagg gcgcctatcg tgcgcggctc ctccttggag
     1261 aggatggcga cgcccgagta gccgcggcgc ttggcggagg cgtagtaggc gcggtaggcc
     1321 ccgccgttgc cgtcggacgg ggcgaggaat tccttgggga gctgctcggg atgggccttc
     1381 gtctcctgca ggcagaggat gtcgggggaa tcggcggcga gccagtcgaa gaggcccttg
     1441 ccggcgcagg cgcgcacgcc gttgacgttg aaggagagga tccgcgtggt catggctctg
     1501 gtgtccctct ggggcctcta ggggctctcc tggagctgga gctccacgga gaggacgagg
     1561 cggtaggtct tgccgcggta ggcggtgaat ttgcgcgaga cgacgtagtc gccgatcctg
     1621 caggacaggt tgtgctcccc gggctcgacg gcgaaggagg gcttggccag gaagtcgatc
     1681 ctggccccgt ccaggctgag ctgggcggag tcgggcgcct cgatgacgag gaggggcatc
     1741 gtgtcctgga gctcgaccgc gagctccagg atccggccct gctccacctg gaaggagcgg
     1801 ctctcgtccc ggaagcagtc ggaggagacg cggagggaat ggcttccggc cttcaggggc
     1861 agcagctcct tgccgtcctg gtagcggccc tcgggaaggc gcttgtcgtc gacgaagacg
     1921 gcgacgcccg cgcgctcggc cccctcgggg tagcggagca tgaggcgcag ggcgccctcg
     1981 tcggtgagga tggggcggac gcggacctgg aactgcgccg cctcgagctc gggggagagg
     2041 cccttggtga cggggaagag cctgaagacg agggggaagt ccttaggctc ggcgacgacg
     2101 gggaggaggg tggcgtaggg gccgctcttg agcgaatggt ccttgcgcac ggggacctgg
     2161 atgacgatgc cggccctggc gggcagggcc tgggtcagga tcctcgagcc ctcgtagccg
     2221 tagcggttct tgtcgggcag ggggtcgacg cggcgccaga gctcgtaggc cagggcgccg
     2281 gggaaggcca gggcggcctg gggggacttg agctcgatct cgaagccctg gatgaagggg
     2341 gaatccttgg agaggaggat ggcgaagccg tcgttgtagc cgatggagag cctcgtgcct
     2401 tcggaggcct cgggggagag gctcaccgtg cccgcgacga tggtcctgag ctcctccgcg
     2461 ccgacggcgg cccggcccgg cgcgaacagg gcgagaagga ggagaactgc tgccggggcc
     2521 gcgcgctttc gggcaatcat ggcttcacct tcgggatgag gggctcggga ttccgcggct
     2581 ctcccctgct gcgcacctcg aaatggaggt gcgggccggt ggagaggccc gtcgatccta
     2641 ctcgcccgat catcatactc gattcgacgc ggtcgttcaa cctcgcgtat cgcgtcgaaa
     2701 gatggccgta gacggtctgc cagccgccct cgtggtcgac gacgatgaac tcgcccagga
     2761 ccggatccac ccccgaggcc gacaccctgc cctcccgcgc ggcgtagacc tcggtgcccg
     2821 acggcgcggc caggtcgatg ccggagtgca tggccgtccg gccgctcacc gggctcgtcc
     2881 tgaggccgaa gccgctcgtg agcctggccg cgggcagggg caggcggaag aggaggccga
     2941 ggaagagggc ccgctcctcg gcgttgaagc gctcgccgcg gtagaagaga aggcttaagg
     3001 cccccctcgg cccctcgacg gagaggctcc ttcctcccgc ctcgccgcgg taggagatga
     3061 ggaagtcgag gtcggtcctg ggcttggccg gggcgaagag gcccggagcc gaggggacga
     3121 ggatggtctc gccgcgctcg aagggccgcg cccggtccag gccgttcagg gtcgcgaggg
     3181 tctcgtaggg caggttcagg cgggcggcga tggagaggag gtccaggacg ttcctcgccg
     3241 tgtaggagta gaggatgagc tcgggggccg ggccgcccga gctcaaggcc gcgtaggagg
     3301 cggcgagctg ctcctggtgc tggcggtaga tcggatcgtc ggggccgagg ctcccgaggc
     3361 tcgggtagga agccttcgcg gcggcgagga tggccttggg gggctccgac gcgagggccg
     3421 aggccgagga aagaaggaag gcaagggcga ggaggaggcc ccgccccgcc cgctcgggac
     3481 ggcgcggccc gaggctcatg ggcgcggcgc cggggattcg ccaagccccg cgtcgcggag
     3541 ctgatcgtcg cggagcttcg cgtcgcggac cttcgcgccg cgaaggtccg cgccgcgagg
     3601 atccgcgccg ggagccgcgc cgcgaggctt tgcgccccgc cggcccgcgc cctggcgtct
     3661 cgcgccaagg cgcctcgcgg cggcgaaggc gagaagggcc agggccaggc cgcccatgag
     3721 ggcgttgaag agggaccttc gctccgtcgc cagggcccag aggggccaga ccgccgccgc
     3781 agaggccacg acgatgccca ggcccgcgag gaggacatgg gcgaggcgct cggcggcccg
     3841 ggcagagcgg tctccccgtt cgagggccct cgacgcggcc tcccccgccc tttcggcctt
     3901 cctgctcacg gcttcgcctc cgcgtccggg aagccccgct ccaggacggc gcggcccttc
     3961 aggaagaggg cggagagctc ggcctcgcgg aggccgcggc cggagagcct ccgccgtggg
     4021 tcgaggcgcg agagccggcg gcgcgcggcg gcctcggtct cggcgacggg ctcgggcccg
     4081 aggagtcccg acttggcgag ctcgcggccg cagagctcgg tgaggaagcc gagctccagg
     4141 aagtcgtcgg aggagagggc gggcggctcg gcggcgagcc aggcctccag ggcggcgccg
     4201 tcggcgacct tgcccccccc ggcgaaggcg gccgcgccca gggaatcggc cgcggcgacg
     4261 cccagggcgg ggaaggcctc ctccagccag gactccgcga gggcgaccag gcggctctcg
     4321 gtgaagtagg cctcgccgag ggcccggcgg agggcggcgc cgagctcggc cgtccgcgga
     4381 gaggagaggg cgcgccgacg gaggacgctc ctggccgcca gggccgcgag gctggcgccg
     4441 gcgagctcgt cgacgagggg aatgggatcc cgcaccacga tggagagaaa gaggtagacg
     4501 aagaggaagg cgcccgagac ggcggcgacg cggaggacga agcccgagtc gagggccctc
     4561 tccctgaggc cggcctcgag ctcggcgtcc agggcgccgc ggagctcgta gaaggaggag
     4621 ggcgaggggc cggcggcgaa gcgcccctcg aagcgcggag gctcgggcgc ggcctccgcc
     4681 tccgcgtaga gcggcgagcc ggggccgaag ggatggatga cgaggggtcc ctccggaccg
     4741 gcgcggagga cgaagaggct cgtggtggcc gaggtcatgg agccacccta gacggagcct
     4801 tcccggacgt caaggggagg ggggatcgcg gcgggactcg tcctggccgc catcgccgag
     4861 caccgagtcg acgaggtcgc gcagggaggg gtccaggccg ccgaggctcg ttccgtagcc
     4921 ggcgtcgagc tggtagaggc cgtcgcggta gacgatgggg ccgccctggg gatcggcggg
     4981 gacctcgtcc tcgtcctcgg tggcggggaa gtcgaaatcg ggggactcga ggagctcgcc
     5041 ctcggcctcg ccctcctcgg cctcctcgac gacgggcagc tcgccctcga cgggggcgcc
     5101 catgaagggg ccgcgggccc tggagggagg cgaataggcc ctgaaggcgc ccagggaagc
     5161 cccgagctcc gccgcctgct cgcccgactc ctccgccgtc gcgctctccg cgaggggaag
     5221 ctcggccggg gcggcttcgg ccgccgggga agcggcagtc tcggggactt ccgccgcggg
     5281 cgcgacgggc gaggacccta caggcccagg cgcggcggcc gcgacagccg caggcgggac
     5341 agccgcaggc gcgacaggtt cgtgcgcgat cggctcgata ggctcggctt ccacgggctc
     5401 gagttcctcg ggctcgacaa gctcagcctc tacgggctcg agctcctcgg gctcggcctc
     5461 gaccggttcg agctcggcgg gcgcggcctc gaggccaggc gccgccttgg cggccgggac
     5521 cgacggcacg gcggcagcca cggcgggcga cggcgtaccg gccgcctcgg cggaggcggc
     5581 cgcggcaact tcctcgcgat agtccgaacg cgtcgcgtcg atctcgtcgg cgccggcggg
     5641 accgcggtcc cactgctcga gggaggagag gtcgagggag ccgaggagga gctcgaactc
     5701 catctcctcc aggggcacga tgacgggctc gggctggaag accacgggcg tctcgggctc
     5761 ggggggcggc gcgggctcgc cggcgaactc cgcctcctcc aggacggcga gctcgggcac
     5821 ctcctcaagc gcctcctcca gggccgcgag cttgcgcgcc tcggcgtcgg ccagggggac
     5881 ggcccgtccg aggacttctc cctcagcggg accggcgaag tcgaggtaag ccgagcggct
     5941 cgtgtcctcc atgtcgatga agccgatgat gtctgctatg tcggcctcgt cggccagctc
     6001 gagtccgctc gactccggga tgagggggat gtcctccgac tcgtcccgcg cgagctcgag
     6061 ggcgcgggcg gcggcccggc gcaggtcgcc gaaggccact tcctcctcca ccgcgagggc
     6121 gccgtagtcg aactcggact ccgcgggctc cagggtctcg agctcctctg cctcgccgcc
     6181 ttcagccgcc gattcgacct cgacgggctc aagctcctcg caggcctcga gctcctcgag
     6241 ctcttcgggc tcgccgcctt cggcggccgc gggcgcgtgg acttcctctt ccgcggcggg
     6301 cttgagggca ggctggacgc ggggaggcgc ttcgaagggg aacgccggca tctgctcgaa
     6361 cgccgcacct gcggcggcct ggggcaccgg ctcggcggca gcctcctcgg cttcctccat
     6421 ctcctcgagc gcttccagct cctcgagctc ctcaagctct tcggcttcgg cgatttccac
     6481 ggcctcgggg gccgccgcgg gctcggcttg ccccgccggc gcaggggcca ccgccggctc
     6541 gaccgctccg gcggccgggg cctcgggctc gccctcttcc agctcctcaa gctcctcgag
     6601 ctcttccagt tcctcgagct cctcgagctc ctcggcctcg gcggcttcgg caggctcggc
     6661 ggcctcgggg atctcgatcg ccacggccgg ctccggcgtc gcggcggcga ctgccggcga
     6721 aggcgcggcg gcggcggccg gcgaaggcgc ggcggcggcg gccggcgaag gcgcggcggc
     6781 cggcgcctcg gcctcgccct cctccagctc ctcgagttcc tcaagctctt ccagttcctc
     6841 gagctcctcg acctcggcct cagtcccagg gccggtctcc ttcgccgccg cgggcgcggg
     6901 gatagcggcg acctcctcgg cctcgccaag ctcctcgagc tcctcgagct cttccagctc
     6961 ctcggcctct gccgcggcct caggctcgag ggagggggga gcggcgaggg tcggaggcgc
     7021 ggcgcggggc tcggcctgga cggctacggg cttggcctgg gccttgggag ccggagccgc
     7081 ggccgcgcgc ggagcggcct gcggaagggc ggactccgcg aggacggcgg ggggaaggac
     7141 gaagctcccg ttcttgaggg cctcggcgat gaggcgctcg acttccttga
//
LOCUS       DAOOSJ010000136         7182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6483_length_7182_cov_6.059071, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000136 DAOOSJ010000000
VERSION     DAOOSJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6483_length_7182_cov_6.059071"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLG14_07705"
     CDS             <1..1267
                     /locus_tag="PLG14_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX49060.1"
                     /translation="ASFLSAAAAASAESLGVEAAVAKALDGNPGLYRARLDLEAAGRA
                     AAAAYNLLYPDASLGAGLSRSSSDRIATESERYTLSGSLSLSLSLSPALAQKRKASRL
                     AYEAALLSYEAQARALELELRSSFDAILLAKARLGLVLQNQERQRKSHEQVAARYAAG
                     LASELDLLSARYSYEASGPAVEAARLALENGLDSFRLLLGLEPGSPLDLAGDLASARA
                     VTGEAARAALEAAKAKERPSVALRQKALESARAAERLASLGVASPSLSLSARLSPSAP
                     LGGPVSDSGSVSLGLSLPLDGLLPASAERLALAAARDAVAKAESSLQEAARASAAKAL
                     QAVRAIEAAEAALASLDRNVALAQKKYDLTLAAYQQGLKSLSDLEAAASGLDSARVDA
                     LAQEYALLTKALELENELGLGFGGVGRME"
     gene            1270..2454
                     /locus_tag="PLG14_07710"
     CDS             1270..2454
                     /locus_tag="PLG14_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX49061.1"
                     /translation="MKKRTAFALALVAALAAAAVVATLVLGGKGGGAADPMAGLSQAE
                     RTLVQSLGPDQRKAVLEAYAAAKAQGRKARMADFMPEAVYSVQEGRAERRTLRSYLDL
                     SGDVVTRRSVVAYPDIGGRVASVAAKLGAFVPEGGLIAQVDPSSPGSSYALSAVRAPI
                     AGTVTSLAVQVGTRVTTASAVATVGVLDELEVDVLVRERDVALVRAGQRAVLRFEAFP
                     GESFEAQVAGLSPVLDPVSRTRTATLSFASPDRRVQPGMYARVRIYTEALSGVVALPE
                     AAIFERFGERYVYVIAAKEGADRVELRKIEKGVVVDGVAEAKSGLAAGERVVTAGRTA
                     LSDGALVRVVSGRGAARNGSPAPGGAPAAPGQGSPAPGASGGAPASGTAPASGGPPGG
                     GK"
     gene            2451..5543
                     /locus_tag="PLG14_07715"
     CDS             2451..5543
                     /locus_tag="PLG14_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX49062.1"
                     /translation="MSLAKRVVGRPVLITVLFALVTIVGIYSTLQLPLDLFPASEEPR
                     MSVSTSWTGANPRTVEELVTKVLEASLTGVEGLSSVSSTSSEGSSNISLAFEYGTDLD
                     AATNDIRDKLGRARRALPDGVGEPTIQKFDSSAMPIMRLAIRGSRTAEELKAIAESAV
                     APRLEQAAGVAQVSVSGERARVVRADVSRNRLEAYGLTITQIASSLAAQSVETGGGRI
                     EEGDKSYSLRTTGEFASIAQIADAVVARRNGYGVRLRDVAEVYDGFAEETSAVLIDGE
                     TGVYLSVQKRTGANSIQTADAVAAKLAEVSRSLPAGVEVETISDDTRLIRSTVADLVS
                     SAGAGALLAMAFVFLFLRSLRPTIVIGLSIPVSIVVTLLAMYFAGLSLNMMTLTGLIL
                     GVGMIVDSSIVIIDNIHAYRERGARARVAAMVGTEEMILPITASTLTTIVVFLPMILF
                     RRKLGRLAVMFEGAMFTIVIALVASWIIALFLVPVLASRYLPFATREERPIRSRLLLA
                     LDRAVEGALSALTRGYSAALKAALGHRALSVLVALGLFALALAAAPRMDIVFSPTMQG
                     DSVSLSVSLPVGARYEETKDLVLQLEAAARADIKGYKTVIASVGSSGRSGRSNQGSID
                     VILPPDGKGADSAQAVQAKLRSRLKDYPQATFSFSSGRGGFGSRPDLSIAVTSEDLEK
                     AVATADGIVALIKAKVPQVTDARTDMTNGLPQLQIVIDRERAYSFGLSLSAVAKEIDA
                     NVAGVTAAAYEEGGEELGVFLSLAKEDRQSVPDLGSIFVLSSSGAHVPLSEIASMEKG
                     VGPVSIRRENQARKVTVTGKLSGLRADKAEALVKEAIRDGLVLDDAVRLEYSGSWSEI
                     TSTGSSFLLVLALAVLLVFGVMAGQYESFKDPFINLFTIPLALIGVFAVHLLAGQAFS
                     MFTLVGLVMLVGIVVNNGIVLVDYTNLLRGRGRPLFEACLEAGRSRLRPVLMTAVTTI
                     LGVAPMALFPTQNASIMQPIGLCIMGGLASSTFITLLLIPVVYYLFNAKEEARKEAAR
                     "
     gene            5540..5812
                     /locus_tag="PLG14_07720"
     CDS             5540..5812
                     /locus_tag="PLG14_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49063.1"
                     /translation="MKRLELIAGESMQAELVEALEAALPGIEYTLIPRALGKGASSRR
                     EGTNVWPEENFVLFSYLGEAEAERALAAVESVRSRFPAEGVAVFLL"
     gene            complement(5954..>7182)
                     /locus_tag="PLG14_07725"
     CDS             complement(5954..>7182)
                     /locus_tag="PLG14_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOX49064.1"
                     /translation="LVLALTAPRGPEDWLFDSRTVAARDANGDGPMHHAARRGLAAAL
                     SFLRDKGADPEARNADGKSPLAAAVEADQAEAAKVLVGMGASLQARDAMGATPLHAAV
                     LWSARRCLPLLLAAGAETDARDFAGEAPLHLAARKRDLPSLKALVAAGAPLSPRESRG
                     ATPLMLAARTGAADIAAALLDSGADPEERDLSGRGALLAAVDAGEAETARLVAKAGAS
                     ILSRDGEGESPLTLAAKRSLSLLDILLSPEAVNRADSEGRAPLRILVESKPSVEIVEL
                     ALARGARPDARDRFSSTALHAALRAGNFPVAALLAGAGADLFARDREGDSPASLAVAA
                     GQDALAALFGGNRLGAADPSGNTALHYAALAGRGEAVSWLLSAGADRTARNIGGETAA
                     DLAEKRGAKELAATLR"
BASE COUNT          933 a         2708 c         2532 g         1009 t
ORIGIN      
        1 ggcctcgttc ctgtccgccg cagcggccgc ctccgcggag agcctcggag tcgaggcggc
       61 cgtcgcgaag gccctggacg gcaatcccgg cctctaccgc gcccgcctgg acctggaggc
      121 cgctggccgc gcggccgcgg ccgcctacaa cctcctctat cccgacgcga gcctgggcgc
      181 ggggctctcc cgctcctcct cggaccgcat cgctacggag agcgagcgct acaccctctc
      241 gggcagcctc tcgctctccc tgtccctctc gccggccctc gcccagaagc ggaaggcctc
      301 ccgcctcgcc tacgaggccg ccctcctctc ctacgaggcc caggcccggg ccctcgagct
      361 cgagctcagg agctccttcg acgcgatcct cctcgccaag gccaggctcg gcctcgtcct
      421 ccagaaccag gagcggcaga ggaagagcca cgagcaggtc gccgcgcgct acgcggccgg
      481 cctcgcgagc gagctcgacc tcctgtccgc gcgctattcc tacgaggcct cggggccggc
      541 cgtggaggcg gcccgcctgg ccctggagaa cggcctggac tccttccgcc tcctcctcgg
      601 cctggagccc ggctcgcccc tcgacctcgc gggcgacctg gcctcggccc gggccgtcac
      661 cggagaggcg gcgagggcgg ccctggaggc cgcgaaggcc aaggagaggc cgagcgtcgc
      721 cctccgccaa aaggccctgg aatcggcccg cgccgccgag cgcctcgcct ccctgggcgt
      781 cgcgtcgccc agtctctccc tctccgccag gctctcccct tcggcgcccc tgggcggccc
      841 cgtctccgac tcgggctccg tctccttggg cctctccctg cccctggacg gccttcttcc
      901 cgcctcggcc gagaggctcg ccctggcggc ggcccgggac gccgtggcca aggccgagag
      961 ctccctccag gaggccgccc gcgcctcggc ggccaaggcc ctccaggcgg tccgcgccat
     1021 agaggcggcg gaggccgccc tggcaagcct ggaccgcaac gtcgcgctgg cgcagaagaa
     1081 gtacgacctg accctcgcgg cctaccagca gggcctcaag agcctttccg acctcgaggc
     1141 ggccgcgagc ggcctcgact cggcccgcgt cgacgcgctc gcccaggagt acgccctcct
     1201 tacgaaggcc ctggagctcg agaacgagct cggcctcggc ttcggcggcg tcgggaggat
     1261 ggaatgagca tgaagaaacg gacggccttc gccctcgccc tggtcgcggc cctcgccgcg
     1321 gccgccgttg tcgcgacact ggtcctgggc ggcaagggcg gcggcgcggc cgatccgatg
     1381 gcggggctct cccaggcgga gcggaccctc gtccagagcc tgggccccga ccagcgcaag
     1441 gccgtcctcg aggcctacgc cgccgccaag gcccagggca ggaaggcgag gatggccgac
     1501 ttcatgcccg aggcggtcta ctccgtccag gagggccgcg cggagcgcag gaccctgcgg
     1561 agctacctgg acctctcggg cgacgtcgtc acgaggcgga gcgtcgtcgc ctatcccgac
     1621 ataggcggcc gcgtcgcctc cgtcgccgcc aagctcggcg ccttcgtccc ggagggaggc
     1681 ctcatcgccc aggtcgaccc ctcctcgccc ggctcctcct acgccctctc cgcggtgagg
     1741 gcgcccatcg cgggcacggt gacttccctc gcggtccagg ttggcacccg ggtcacgaca
     1801 gcctcggccg tcgcgacggt cggcgtcctg gacgagctcg aggtggacgt cctcgtgcgc
     1861 gagcgggacg tggccctggt cagggccgga cagcgcgccg tcctcaggtt cgaggccttt
     1921 cccggagaga gcttcgaggc ccaggtcgcc gggctctccc ccgtcctcga cccggtctcc
     1981 cggacgagga cggccacgct ctccttcgcc tcgcccgacc ggcgcgtcca gccggggatg
     2041 tacgcgaggg tcaggatcta caccgaggcc ctctcgggcg tcgtggccct tccggaggcg
     2101 gccatcttcg agcgcttcgg ggagcgctac gtctacgtca tcgcggcgaa ggaaggcgcg
     2161 gaccgcgtcg agctccgcaa gatcgagaag ggcgtcgtcg tggacggcgt cgccgaggcc
     2221 aagtcaggcc tcgcggccgg cgagcgcgtc gtcaccgcgg gccgcaccgc cctgagcgac
     2281 ggggccctgg tgcgcgtcgt ctcgggccgc ggcgccgcgc ggaacggctc gcccgcgccg
     2341 ggaggcgcgc cggccgcgcc gggccaaggt tcgcccgcgc caggcgcgtc gggcggcgcg
     2401 cccgcgtccg ggacggcgcc cgcgtccggc ggcccgcccg gaggcggcaa gtgagcctcg
     2461 cgaagcgcgt cgtcgggagg cccgtcctca tcacggtcct cttcgccctc gtgacgatag
     2521 tcggcatcta ttcgaccctc cagctgcccc tcgacctctt ccccgcgagc gaggagccgc
     2581 ggatgtcggt gagcacctcc tggacgggcg ccaacccgcg cacggtggag gagctcgtca
     2641 ccaaggtcct ggaggcgagc ctcacgggcg tcgagggcct ctccagcgtc agctcgacct
     2701 cgtcggaggg ctcgagcaac atctcgctcg ccttcgagta cggcacggac ctggacgcgg
     2761 cgacgaacga catccgcgac aagctcggcc gggccaggcg ggccctgccc gacggcgtcg
     2821 gcgagcccac catccagaaa ttcgacagct ccgcgatgcc gatcatgcgg ctcgcgatca
     2881 ggggctcgcg gacggccgag gagctcaagg cgatagccga aagcgccgtc gccccgcggc
     2941 tcgagcaggc cgccggcgtg gcccaggtct cggtctccgg agagcgggcc cgagtcgtga
     3001 gggccgacgt ctcgcggaac cgcctggagg cctacgggct cacgatcacc cagatagcct
     3061 cctccctggc ggcgcagagc gtcgagacgg ggggcggccg gatcgaggag ggcgacaagt
     3121 cctacagcct gcgcacgacg ggcgagttcg cgtcgatcgc gcagatcgcg gacgccgtcg
     3181 tggccaggcg gaacggctac ggcgtgaggc tccgcgacgt cgcggaggtc tacgacggct
     3241 tcgcggagga gacgagcgcc gtcctcatcg acggcgagac cggcgtctac ctctcggtcc
     3301 agaagcggac gggcgcgaac tcgatccaga cggccgacgc cgtcgccgcg aagctcgcgg
     3361 aggtctcccg gtccctgccc gcgggggtcg aggtggagac catctccgac gacacgaggc
     3421 tcatccgctc cacggtggcc gacctggtct cctcggcggg cgcgggcgcc ctcctcgcga
     3481 tggccttcgt cttcctcttc ctcaggagcc tccggccgac catcgtcatc ggcctctcga
     3541 tcccggtgtc gatagtcgtc accctcctcg ccatgtactt cgctggcctc tccctcaaca
     3601 tgatgaccct cacgggcctc atcctgggcg tgggcatgat cgtggactcc tccatcgtca
     3661 tcatcgacaa catccacgcc taccgcgagc gcggcgcgcg ggcccgggtg gccgcgatgg
     3721 tcggcacgga ggagatgatc ctccccatca ccgcctccac cctgaccacg atcgtcgtat
     3781 tcctcccgat gatcctcttc aggaggaagc tgggcaggct cgcggtcatg ttcgagggcg
     3841 cgatgttcac catcgtcatc gccctggtcg cgagctggat catcgccctc ttcctcgtgc
     3901 cggtgctcgc gagccgctac ctccccttcg cgacgcggga ggagcggccg atcaggtcgc
     3961 gcctcctcct cgcgctggac cgggcggtgg agggggccct gtcggcgctc acgaggggct
     4021 acagcgccgc cctgaaggcc gccctgggcc accgggccct ctccgtcctc gtcgccctcg
     4081 ggctcttcgc cctggccctg gcggccgcgc ccaggatgga catcgtcttc agcccgacca
     4141 tgcagggcga ctcggtgagc ctctcggtct ccctccccgt gggcgcgcgc tacgaggaga
     4201 cgaaggacct ggtcctccag ctcgaggcgg cggcgcgggc cgacatcaag ggctacaaga
     4261 ccgtcatcgc ctcggtcggc tcctccggcc gctccggccg ctccaaccag ggctccatcg
     4321 acgtcatcct gcctcccgac ggcaagggcg ccgactcggc ccaggcagtc caggcgaagc
     4381 tccgctcgcg cctcaaggac tacccccagg ccaccttctc cttctcctcc ggcaggggag
     4441 gcttcggctc caggcccgac ctctccatcg ccgtcacctc ggaggacctg gagaaggcgg
     4501 tcgcgacggc ggacggcatc gtcgccctca tcaaggcgaa ggtcccgcag gtgaccgacg
     4561 cgaggaccga catgaccaac ggcctccccc agctccagat cgtcatcgac cgggagaggg
     4621 cctactcctt cggcctctcg ctctccgcgg tcgcgaagga gatcgacgcg aacgtggccg
     4681 gcgtcacggc cgcggcctac gaggagggag gcgaggagct cggcgtcttc ctctccctcg
     4741 cgaaggagga ccggcagtcc gtccccgacc tgggcagtat cttcgtgctc tcctcctccg
     4801 gggcccacgt ccccctctcg gagatcgcgt ccatggagaa gggcgtcggg ccggtgagca
     4861 tccgccggga gaaccaggcc aggaaggtca cggtcacggg gaagctctcc ggcctcaggg
     4921 ccgacaaggc ggaggccctg gtcaaggaag cgatccggga cggcctcgtg ctcgacgacg
     4981 cggtccggct cgagtactcg ggctcctggt cggagatcac ctccacgggc tcctccttcc
     5041 tcctcgtcct ggccctggcc gtcctcctcg tcttcggcgt gatggcgggc cagtacgagt
     5101 ccttcaagga tcccttcatc aacctcttca cgataccgct cgccctgata ggcgtcttcg
     5161 ccgtccacct cctggccggc caggccttca gcatgttcac gctggtcggc ctcgtgatgc
     5221 tcgtcggcat cgtcgtcaac aacggcatcg tcctcgtcga ctacacgaac ctgctgcggg
     5281 gccgcggccg gcccctcttc gaggcctgcc tggaggcggg ccggagccga ctgcgccccg
     5341 tcctcatgac ggcggtgacg accatcctgg gcgtggcgcc gatggccctc ttccccaccc
     5401 agaacgcctc gatcatgcag cccatcgggc tctgcatcat gggagggctc gcgagcagca
     5461 ccttcatcac cctcctcctc atccccgtcg tctactacct cttcaacgcg aaggaagagg
     5521 cccgaaagga ggccgcgcga tgaagcggct cgagctcata gcgggcgagt cgatgcaggc
     5581 cgagctcgtc gaggccctgg aggccgccct gcccggcatc gagtacaccc tcattccgcg
     5641 cgccctgggc aagggcgcaa gctcgaggcg ggagggcacc aacgtctggc ccgaggagaa
     5701 cttcgtcctc ttctcctacc tcggcgaggc cgaggcggag agggccctcg ccgccgtcga
     5761 gtccgtgcgg agccgcttcc ccgcggaggg cgtcgcggtc ttcctcctct aggccggccg
     5821 ccgggcggcg cgtctccgac gctttgcggc gctcaatgcg cgccttccgt cgcgcgcgta
     5881 ccctaggacg acaagaagcc gtccggcccg agggccggac ggcttcttgt ttcatgcgcc
     5941 gcgactgcgg tcgtcagcgg agcgtcgccg cgagctcctt cgcgccgcgc ttctccgcga
     6001 ggtccgcggc cgtctctccg ccgatgttgc gggccgtcct gtcggcgccg gccgaaagga
     6061 gccaggagac ggcctccccc ctgccagcga gggcggcgta gtggagtgcg gtgttgccgc
     6121 tcgggtcggc cgcgcccagg cgattgcccc cgaagagggc ggccagggca tcctgtcccg
     6181 cggccaccgc gagggaggcg ggggagtcgc cctcgcggtc gcgcgcgaag aggtcggccc
     6241 ccgcgcccgc gaggagggcg gccacgggga agttgcccgc gcggagggcc gcgtgcaggg
     6301 ccgtcgagga gaagcggtcg cgcgcgtcgg gcctcgcgcc gcgggccagg gccagctcga
     6361 cgatctccac ggagggcttg gactcgacga ggatgcgcag gggcgcgcgg ccctcggagt
     6421 cggcccggtt cacggcctcc ggggagagga ggatgtcgag gagggagagg ctccgcttcg
     6481 ccgccagggt gaggggcgac tcgccctcgc cgtcgcggga gaggatggag gccccggcct
     6541 tggcgacgag gcgggcggtc tcggcctcgc ctgcgtccac ggcggcgagg agggcgccgc
     6601 ggcccgagag gtcccgctcc tccgggtcgg cgcctgagtc caggagggcg gcggcgatgt
     6661 cggccgcgcc ggtcctcgcc gccagcatga ggggcgtcgc gccgcggctc tcgcggggcg
     6721 agaggggggc gccggccgcg accagggcct tgagggaggg aaggtcgcgc ttccgcgccg
     6781 cgaggtggag gggggcctcg ccggcgaagt cgcgggcgtc ggtctctgcc cccgcggcga
     6841 ggaggagggg gaggcacctc ctggcgctcc agagcacggc cgcgtggagg ggcgtggcgc
     6901 ccatcgcgtc gcgggcctgg agcgaggcgc ccattccgac gaggaccttc gcggcctcgg
     6961 cctggtcggc ctcgacggcc gcggccagcg ggctcttccc gtccgcgttg cgcgcctcgg
     7021 ggtcggctcc cttgtcccgc aggaaggaga gggcggcggc gaggccgcgc ctcgcggcgt
     7081 ggtgcatggg gccgtcgccg ttggcgtcgc gggccgcgac cgtcctggag tcgaagagcc
     7141 agtcctcggg gccgcggggc gcggtgaggg ccaggacgag gg
//
LOCUS       DAOOSJ010000137         7182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6484_length_7182_cov_5.657921, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000137 DAOOSJ010000000
VERSION     DAOOSJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6484_length_7182_cov_5.657921"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="PLG14_07730"
     CDS             complement(<1..2264)
                     /locus_tag="PLG14_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49065.1"
                     /translation="MPLPLPPGLKPLLAAGAALVPETEASLGNPSFADAEARILVVRL
                     SPWRDVDRSTSHLILFAECRKALPRAYLDFAFMPDRGDRELLVGAARGGGTPPWFYGL
                     ASGRSPADFDLVLVSNAFALELLNLPYLFSTSGLPLRASARAALGARRPLVVLGGSNA
                     AAAGAIVGEAGADGSRDAMADGLFFGEGEGAIGELALALTAPGVSPDGRLARAEAVEG
                     FWRAGAPPRGGGTRRRLLGASPEPLLEWPLLNSPESGTARLQIAAGCPGLCSFCFEGW
                     DRRPYRERPLAHIIADAREIRRRQGADSLEVYSFNFNTHEEVFALLFELGRIFRRVSF
                     MSQRLDVLASTPGLVRAELAADKRSFTLGIEGVSARMRAYYRKGLSDEELGRLVELLV
                     APGIRELKLFYIVSGLEEDADLEEFAAFARLLGEARSERAPGLRILASAGYLVRLPWT
                     PLQSAPLALEEAPLARIAGALREAFEASGLEFRLASYFDEYCADQLLVLGGPPLLPWL
                     EAAPSRGLVYDGSLSRGAWPSLRAAAEAAGLLGAEFLSEKGEGWRPPLPFLDPGRDSD
                     LLRAEYLEARAGRDRPACLGEGCRDCGACEDAGARAFIARHRVAPPAEGLIDRITRLT
                     AAKAAFRPVYAEVDLPAGLSGSTEAYKGAYVLKELGRLVPGSEQSVFEAREALFCRGG
                     AFGLGEGFSGKTVFALYGPRPERITEIAAAAGLAVLPAGPGREAPAAVRIEAEVGFPP
                     GEAEARRILAAFRE"
     gene            complement(2284..4098)
                     /locus_tag="PLG14_07735"
     CDS             complement(2284..4098)
                     /locus_tag="PLG14_07735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX49066.1"
                     /translation="MDAFLGLGLSEPLATALSSLGYSLPTPIQEAAIPLLLSGRDVLV
                     ESETGTGKTFAYLAPALELAAFGKRPPKPAPEGGAEGGAAADDAAPAAPAATAAAAAA
                     AAAAAVLEAAPARRAPLGRGASAGREGPEVLVVAPTQELAVQIGRETERLVEASGLGL
                     KVAVILGGTPLSKQEAKLQARPAVVVGTLGRLGDLVALRRIRLSALKLLVLDEADRLL
                     APEAEEQTTKLLSALPRAAARVLVSATLPARVRAKAAPFLTEPALASASAGPVLAGDI
                     EHWCFYCDGRKRLDFLRRLEAAIRPPRCLVFIAQAHRVPHAVERLEAMGLPVAGIHAR
                     LEKEERRVALERFAKGELRYLVTSDLGARGLDIAGLSHVVSLDLPEEPTVYVHRAGRT
                     GRAGAKGVSIVLADSVELERASRMAVRAGFVFRCKFLEAGEVLEPTTVEFFERAERAD
                     AEKQSHRAERSLRGQDAREARPASSGRPEASRPRQERPYPDRSGAERRRPAPGAERPA
                     APRTSPGRPSSSRPAPAGAESARPNAARPEARREEPREAPRGTPRPAPREIRRATPSH
                     GKGPQRPKSLRPGAPGWTQADEDGRSRDAEPGRGKGRR"
     gene            4203..5132
                     /locus_tag="PLG14_07740"
     CDS             4203..5132
                     /locus_tag="PLG14_07740"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOX49067.1"
                     /translation="MNLEAFVLGSGGMMPLPQRSLTSVLVRREGELFLFDGGEGTQVS
                     IRRLNLRWKKITAIFVSHMHADHVTGLPGILMLSSQVDREEPLYIFGPPRIAEYIEQS
                     RRVLDMYINYEIIVREFTGPEVLWKGDGFSIRNFPLRHTKPCYGYALEEESRPGAFHP
                     ERAMELGVPRGPMWSTLQGGSPVEIPGGATVRPEEVMGQARAGRKFSYVTDSLYFPEI
                     AKEVSGSDLLICEGMFETALAESAAEKKHMTAEQAARVAREAGGVKKLGLIHYSPRYT
                     ERDLKRLQDEARAVFPETVLTKDRMAFPIEYVD"
     gene            5212..6171
                     /locus_tag="PLG14_07745"
     CDS             5212..6171
                     /locus_tag="PLG14_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX49068.1"
                     /translation="MIEAIGLRKSYGETEAVRGVSFEAEKGSVLGLLGPNGAGKTSIM
                     KVLTGYHYPDGGRALVCGIDVTEDPVGVKRRVGYLPESAPLYPDLTPAEFLSFAADAR
                     GLEGKARAAAIGRAVEACGIGPVLRRPVEELSKGFRQRLGLAQAILHDPEVLILDEPT
                     TGLDPNQILEIRALVREIGREKTVILSTHVLQEVEAVCSRVVILNEGQVAAQGAPAEI
                     GDALKGEERLRVRLRARSGGLSASRAAGLLKALRGLKAAGEPREEESGSLALELQAAP
                     GGEPGAAVFEWAVENGLVVLEMERRRLSLEEIFVRLTQEGGSK"
     gene            6168..6890
                     /locus_tag="PLG14_07750"
     CDS             6168..6890
                     /locus_tag="PLG14_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49069.1"
                     /translation="MRPTLAIARRELRSAWNSPVAYVLVVGFLVFCAGWLYFVRGFFA
                     AGLADLRPYFGLMPLAFAFLAPAATMRSWAEERRLGTYELLLTMPFSEGELVLGKFIA
                     SLSTLGAALLLSLPVPLTASLLGSFDPGVLAGQYLGALLAAATATAAGEWVSSLAKNQ
                     VSAFVAAASLLLALVLVDRLAAFLRLGGAAAGLAAWLSMSRHFESLSRGVLDGRDLAY
                     YLATSGVFLYLTAWNVRRRKWS"
     gene            6896..>7182
                     /locus_tag="PLG14_07755"
     CDS             6896..>7182
                     /locus_tag="PLG14_07755"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOX49070.1"
                     /translation="MDAKTGKKRSLVLLLLVLAVAAASVLFAERFHARLDLTADRSYS
                     LGAVSKGLYKEIPERLRITYYRSPALAARHPGPRAVEDFLREFEAAGRGRIT"
BASE COUNT          949 a         2596 c         2714 g          923 t
ORIGIN      
        1 cattccctga aggcggcgag gatccgcctg gcctcggctt cgcccggcgg gaagccgacc
       61 tcggcctcga tcctgacggc ggccggcgcc tctcgccccg gcccggcggg gaggacggct
      121 aagccggcgg ccgcggcgat ctccgtgatc cgctcgggcc ggggcccgta gagggcgaag
      181 accgtcttcc cggagaagcc ctccccgagg ccgaaggccc cgccgcggca gaagagggcc
      241 tccctcgcct cgaagaccga ctgctcggag cccgggacga ggcggccgag ctccttgagg
      301 acataggcgc ccttgtaggc ctccgtggag cccgaaaggc ccgcggggag gtcgacctcg
      361 gcgtagacgg gccggaaggc cgccttcgcc gcggtcagcc gcgttatgcg gtcgatcaaa
      421 ccctccgcgg gcggcgcgac gcggtggcgg gcgatgaagg cccgggcccc ggcgtcctcg
      481 caggcgccgc agtccctgca gccctcgccc aggcaggcag gcctgtcccg gcctgccctg
      541 gcctccaggt actcggcgcg gaggaggtcc gagtcccggc ccgggtccag gaaggggagg
      601 ggcggcctcc agccctcgcc cttctcggag aggaattccg cgcccaggag gcccgcggcc
      661 tccgcggccg cgcggaggga gggccaggct ccgcgggaga gggagccgtc gtagacgagg
      721 ccgcgggagg gcgcggcctc tagccatggc aggaggggcg ggcctccgag gaccaggagc
      781 tggtccgcgc aatactcgtc gaagtaggag gccaggcgga actcgaggcc cgaggcctcg
      841 aaggcttcgc gcaacgcgcc ggcgatcctc gccaggggcg cttcctccag ggcgaggggc
      901 gcggactgga ggggcgtcca gggcaggcgc acgaggtagc ccgcggaagc caggatccgg
      961 aggccgggcg ccctctccga gcgagcctcg cccaggagcc gggcgaaggc cgcgaactcc
     1021 tcgaggtcgg cgtcctcctc caggccgctc acgatgtaga acagcttgag ctcgcggatg
     1081 ccgggcgcga cgaggagctc gacgaggcgg ccgagttcct cgtcggagag gcccttgcgg
     1141 tagtaggcgc gcatccgcgc ggagacgccc tcgattccca gggtgaagga gcgcttgtcc
     1201 gcggcgagct ccgcgcggac caggcctggg gtcgaggcca ggacgtcgag gcgctggctc
     1261 atgaagctca cgcggcggaa gatccgcccg agctcgaaga ggagggcgaa gacctcctcg
     1321 tgggtgttga agttgaagct gtagacctcg aggctgtcgg cgccctggcg gcgcctgatc
     1381 tcccgcgcgt cggcgatgat atgggcgagg gggcgctcgc ggtaggggcg cctgtcccag
     1441 ccctcgaagc agaaggagca gaggcccgga cagccggccg cgatctggag ccgcgcggtc
     1501 ccggactcgg gggagttgag gaggggccat tcgaggaggg gctcaggcga cgccccgagc
     1561 aggcgccgcc tcgtcccgcc tccgcgcggc ggggcgcccg ccctccagaa gccctccacg
     1621 gcctcggcgc gcgcgagcct cccgtcgggg gagacgccgg gggcggtcag cgcgagggcg
     1681 agctcgccta tcgcgccctc gccctcgccg aagaacaggc cgtcggccat cgcgtcgcgc
     1741 gagccgtcgg cccccgcctc gccgacgata gcgcccgcgg ccgcggcgtt cgagccgccc
     1801 aggacgacga ggggccgcct tgcgcccagg gccgcgcggg cggaggcgcg gagggggagg
     1861 ccggaggtgg agaagaggta gggcaggttc aggagctcga gggcgaaggc gttggagacg
     1921 aggacgaggt cgaagtcggc ggggctccgt cccgaggcga ggccgtagaa ccagggcggc
     1981 gtcccgccgc ccctcgcggc gcctacgagg agctcgcggt cgcccctgtc gggcatgaag
     2041 gcgaagtcca ggtaggctcg gggcagggcc ttcctgcact cggcgaagag tatgaggtgg
     2101 gaggtcgagc ggtccacgtc gcgccagggc gagagccgga cgacgaggat cctcgcctcg
     2161 gcgtccgcga aggaggggtt gccgagggac gcctcggtct cgggaacgag ggctgcgccg
     2221 gccgcgagga ggggcttgag gccgggcggc agggggaggg gcatgccgat tcccctcgcg
     2281 gatctagcgg cggcccttgc cgcggcccgg ctcggcgtcg cggctgcgcc cgtcctcgtc
     2341 ggcctgggtc cagccggggg cgccgggcct cagggacttg gggcgctggg gacccttgcc
     2401 gtggctcggc gtcgcccggc ggatctctcg gggcgccggg cgcggagtcc cgcgcggagc
     2461 ctcgcgcggc tcctcgcgcc ttgcctcggg ccgcgcggca ttggggcgcg cggattcggc
     2521 ccctgcggga gcgggccgcg aggacgaagg ccggcctggc gaagtcctcg gtgccgccgg
     2581 cctctcggcg ccgggggcgg ggcgccggcg ctccgcgccg gagcggtccg ggtacgggcg
     2641 ctcctggcgc gggcggctcg cctccgggcg ccccgacgag gcggggcggg cctcgcgcgc
     2701 gtcctggccg cggaggcttc tctcggcgcg gtgggactgc ttctcggcgt cggccctctc
     2761 ggcgcgctcg aagaactcca ccgtcgtcgg ctccaggacc tcgccggcct ccaggaactt
     2821 gcagcggaag acgaagcccg cgcggacggc catgcgcgac gcgcgctcga gctccacgga
     2881 gtcggcgagg acgatggaga cgcccttggc gccggcgcgg cccgtgcggc ccgcgcggtg
     2941 cacgtagacg gtgggctcct cgggcaggtc cagggagacg acgtgggaga ggccggcgat
     3001 gtcgaggccg cgggccccga ggtcgctcgt cacgaggtag cggagctcgc ccttggcgaa
     3061 gcgctccagg gcgacgcgcc gctcctcctt ctccaggcgg gcgtggatgc ccgcgacggg
     3121 caggcccatc gcctccagcc gctcgacggc gtggggcacg cggtgggcct gggctatgaa
     3181 gacgaggcag cggggcgggc ggatcgccgc ctccaggcgg cggaggaagt cgaggcgctt
     3241 gcggccgtcg cagtagaagc accagtgctc gatgtcgccc gcgaggacgg ggcccgcgct
     3301 ggcggacgcc agggccggct ccgtgaggaa gggcgcggcc ttggcccgga cgcgggcggg
     3361 gagggtggcg gagacgagga cgcgcgccgc ggcgcggggc agggcggaga ggagcttcgt
     3421 cgtctgctcc tcggcctcgg gcgccaggag gcggtcggcc tcgtcgagga cgaggagctt
     3481 gagggcggag aggcggatcc tgcggagggc cacgaggtcg cccaggcggc ccagggtccc
     3541 gacgacgacg gccggccggg cctggagctt ggcctcctgc ttggagaggg gggtgcctcc
     3601 caggatgacg gcgaccttga ggcccaggcc cgaggcctcc acgaggcgct cggtctcgcg
     3661 gccgatctgg acggcgagct cctgggtcgg ggcgacgacg aggacctcgg ggccctcgcg
     3721 gccggctgac gctccgcggc ccaggggagc ccgccgcgcg ggggcggctt ctagtacggc
     3781 ggcggctgct gcggcggctg cggctgcggc ggtggcggca ggcgcggcgg gggccgcgtc
     3841 gtcggccgcg gctcctccct cggccccgcc ctccggcgcg ggcttgggcg gcctcttccc
     3901 gaaggcggcg agctccaggg ccggcgcgag gtaggcgaag gtcttgcccg tgccggtctc
     3961 ggactcgacc aggacgtccc gtcccgaaag gaggaggggg atggccgcct cctggatggg
     4021 cgtcggaagg gagtagccga gcgaggaaag ggccgtggcg aggggctcgg agaggccgag
     4081 gccgaggaat gcgtccatgc ccctactttc gcacgagggg gggaggcgct acaagggccg
     4141 aaggggcctc ggcccgggcg ggacgttgcc caagcgcccc atatcggcta tagtctcgcg
     4201 cgatgaacct ggaagctttc gttctcggct cgggcggcat gatgcccctc ccgcagcgca
     4261 gcctcacgag cgtcctcgtc cggcgcgagg gggagctctt cctcttcgac ggaggcgagg
     4321 gcacgcaagt ctcgatacgg cgcctcaacc tgcgctggaa gaagatcacc gcgatcttcg
     4381 tctcgcacat gcacgccgac cacgtcacgg gcctcccggg catcctcatg ctctcgagcc
     4441 aggtcgaccg cgaggagccg ctctacatct tcgggccgcc gcgcatcgcg gagtacatcg
     4501 agcagagccg gcgcgtcctc gacatgtaca tcaactacga gatcatcgtc cgcgagttca
     4561 cgggcccgga ggtcctctgg aagggcgacg gcttctcgat ccgcaacttt cccctgcggc
     4621 acacgaagcc ctgctacggc tacgccctgg aggaggagtc ccggcccggc gctttccacc
     4681 ccgagcgcgc catggagctc ggcgtgcccc gcgggccgat gtggtcgacc ctccagggcg
     4741 gctccccggt ggagatcccc ggcggcgcga ccgtccgccc cgaggaggtc atgggccagg
     4801 cgagggcggg acgcaagttc agctacgtca ccgacagcct ctacttcccc gagatcgcga
     4861 aggaagtctc cggctccgac ctcctcatct gcgagggcat gttcgagacc gccctggcgg
     4921 agagcgcggc ggagaagaag cacatgacgg ccgagcaggc cgcgcgggtc gctcgcgagg
     4981 cgggcggggt caagaagctc ggcctcatcc actacagccc ccgctacacc gagcgggacc
     5041 tgaagcgcct ccaggacgag gcgcgggccg tcttcccgga gaccgtcctc acgaaggacc
     5101 ggatggcctt tccgatagaa tacgtcgact agggcgtaac cccggctcac cttccggcgt
     5161 ttccaggcgg tccggcggaa ggccgccggg cgcgggaccc aaggaggagc cgtgatagaa
     5221 gcgatagggc tccgcaagag ctacggcgag accgaggctg tgcggggcgt ctctttcgag
     5281 gcggagaagg gctcggtcct cggcctcctc ggcccgaacg gcgccggcaa gacgagcatc
     5341 atgaaggtgc tcacgggcta ccactatccc gacgggggcc gcgccctcgt ctgcgggatc
     5401 gacgtcacgg aggaccccgt cggcgtcaag cgcagggtcg gctacctgcc cgagtcggcc
     5461 cccctctacc ccgacctgac gcccgccgag ttcctctcct tcgcggcgga cgcccgaggc
     5521 ctcgagggca aggcccgcgc cgccgcgatc ggccgcgccg tggaggcctg cggcatcggg
     5581 cccgtcctca ggcggccggt ggaggagctc tccaagggct tccgccagcg cctcggcctc
     5641 gcgcaggcca tcctccacga ccccgaggtc ctcatcctcg acgagccgac gaccggcctc
     5701 gaccccaacc agatcctcga gatccgcgcc ctcgtccgcg agatcggccg cgagaagacc
     5761 gtcatcctct cgacccacgt cctccaggag gtggaggcgg tctgctcgcg cgtcgtcatc
     5821 ctcaacgagg gccaagtcgc cgcccagggc gcgcccgccg agatcggcga cgcgctcaag
     5881 ggcgaggagc ggctccgcgt ccgcctccgc gcccgctcgg gcggcctctc cgcctcccgg
     5941 gccgcgggcc tcctcaaggc cctccgcggc ctcaaggcgg ccggcgaacc ccgcgaggag
     6001 gagtcgggct ccctcgccct cgagctccag gccgcgcccg gcggggagcc gggggccgcc
     6061 gtcttcgagt gggccgtgga gaacggcctc gtcgtcctcg agatggagcg ccgccgcctc
     6121 tccctggagg agatcttcgt gcgcctgacc caggaaggag gctccaagtg aggcctaccc
     6181 tcgcgatcgc tcgccgggag ctgcgctcgg cctggaactc ccccgtggcc tacgtcctcg
     6241 tcgtgggctt cctcgtcttc tgcgcaggct ggctctactt cgtccgcggc ttcttcgccg
     6301 cgggcctcgc ggacctgcgg ccctacttcg gcctcatgcc cctggccttc gccttcctgg
     6361 cccccgcggc cacgatgcgg agctgggccg aggagcgcag gctcggcacc tacgagctcc
     6421 tcctcacgat gcccttctcc gagggcgagc tcgtcctcgg caagttcatc gcctccctct
     6481 cgaccctcgg ggcggccctc ctcctctccc tcccggtgcc gctcaccgcc tccctcctcg
     6541 ggagcttcga cccgggcgtc ctcgccggcc agtacctggg cgccctcctc gccgccgcga
     6601 cggccacggc cgcgggcgaa tgggtctcct ccctcgctaa gaaccaggtg agcgccttcg
     6661 tcgccgcggc ctccctcctc ctcgccctcg tcctcgtgga caggctcgcc gccttcctcc
     6721 gcctgggcgg cgcggccgcg ggcctcgcgg cctggctctc catgagccgc cacttcgagt
     6781 ccctctcgcg cggggtcctg gacgggcggg acctcgccta ctacctcgcg acgagcgggg
     6841 tcttcctcta cctcacggcc tggaacgtcc ggcgccggaa atggagctag cgagcatgga
     6901 cgcgaaaacc ggcaagaagc gcagcctcgt cctcctcctc ctcgtcctcg ccgtcgcggc
     6961 ggcctcggtc ctcttcgccg agcgcttcca cgcgaggctc gacctcaccg ccgaccggag
     7021 ctacagcctg ggcgccgtct ccaaggggct ctacaaggag atccccgagc ggctccggat
     7081 cacctactac cgctcccccg ccctggccgc gaggcacccg ggccccaggg cggtggagga
     7141 cttcctccgc gagttcgagg ccgccggccg ggggcggatt ac
//
LOCUS       DAOOSJ010000138         7146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6518_length_7146_cov_5.576787, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000138 DAOOSJ010000000
VERSION     DAOOSJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6518_length_7146_cov_5.576787"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1207
                     /locus_tag="PLG14_07760"
     CDS             230..1207
                     /locus_tag="PLG14_07760"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOX49071.1"
                     /translation="MAVLIRSIGTHVPERRVANADLAAMVDTTDEWIVSHTGIKARHF
                     AADGMLTSDISAEAARVALERAGLGPKDIDLLIVATATPDYFGFPSTACIVQDKIGAY
                     GCAAFDLGAGCTGFIYALDAAAAMLEARKGRNALVIGAETLSRIVDWKDRATCVLFGD
                     GAGAAVLSRIDETGRGLIASVLGAEGSGHKDLYLVQEERAKTFERSEPIVPAIAMNGQ
                     NVYNFAVKTITVLVSRLLHQTVYKLEDFAWIVPHQANARIVQAAAKRLKAPEEKFYLN
                     MEEYANTSAASIPLALDEMDRKGLLKKGDLIMLIGFGAGLTYGAQAIRW"
     gene            1293..2597
                     /gene="fabF"
                     /locus_tag="PLG14_07765"
     CDS             1293..2597
                     /gene="fabF"
                     /locus_tag="PLG14_07765"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOX49072.1"
                     /translation="MNKRVAITGLGVVSPVGNDVPAFWDSIKAGRSGAGTVTRFDPSR
                     LESRVACEVKGFDPSLYMDKKEARKMALFSQFAVAAAVQAWRDGGLPERSQAEEPGSG
                     PASVSGMRGPYPFDRIGAVIGNGIGGIDVCLESHKKLLESGPGRMLPMTVPLMIANEA
                     AGNVAMRLGIGGPALTAVTACASGSDAIGQALDLIRAGRCDAILAGGTEAAITEFAMG
                     GFCRLQALSTAYNDSPEKASRPFDKARDGFVIGEGAAVLLLEDWDKAVARGARIYAEL
                     AGYGASCDAYHLTAPDPGAEGCARAMRAALADAGMRPEEIAYYNAHGTSTAINDPVET
                     LMLKKAFGEHAKRLKVSSTKSMTGHCIAAAGAIEALVCVKAVEEGFAPPTINLENPDL
                     EAGCDLDYVPNKGVAMPIEAAASGSLGFGGHNAILVVRKARK"
     gene            2594..3046
                     /locus_tag="PLG14_07770"
     CDS             2594..3046
                     /locus_tag="PLG14_07770"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOX49073.1"
                     /translation="MIVDTVPGKALRAEIEGYLPHRAPFALVDEVEVSEGHIRGAMVF
                     PADSFFFAGHFPGYPVVPGVLLVESMAQCGGAGVKRMGLVPNGTFFLAKVKEARFRRQ
                     VRPGERFHMEIDNIKATPNIVHQRGVGYVDGEPAVEAEWLCIAGGNVA"
     gene            3043..4281
                     /locus_tag="PLG14_07775"
     CDS             3043..4281
                     /locus_tag="PLG14_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HOX49074.1"
                     /translation="MIQKPMVRSNICINAHPLGCERETLAQVEYVKARRSLYEGRGGP
                     KAVLVVGASTGYGLASRIAAAFGFRAATVGISFEKEPSATKPGTPGWYDNRTFDAAAR
                     AEGLPSRSLDGDAFSKEMKERAVQAARELDVKYDLVVYSLASPVRTDPATGELFKSAI
                     KPLGKTYTGRTVDVFSGKISEASIQPATEEEAASTVKVMGGEDWALWIEALREGGVLA
                     PGATTVAYSYVGPSLSWPIYRDGTIGRAKAHLEATAKELSARYAKEGLAAYVSINKAV
                     VTRASAVIPVIPLYVSTLFKVMKEKGSHEDCVAQIDRLYRDRLYAAAPSGARRVPPLD
                     AEGRIRIDDLEMSPGVQAEVEARMAHVTEENLRDLTDVEGFRLDFLRIHGFEVPGVDY
                     EAEVAPDYDPAGPAASGAAR"
     gene            4634..5431
                     /locus_tag="PLG14_07780"
     CDS             4634..5431
                     /locus_tag="PLG14_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOX49075.1"
                     /translation="MLILEPGEPGALIPRSDRRCEHVRKILKKKPGDRLLAGIATDSP
                     FGPAPGALGEAVVESLDERGLVLSFEPFPGPRSEPEGLAPLRLILGFPRPIQANRIFK
                     DLASLGVAEIVLTGTELGEKSYLESDFFRAGEFRRPLLEGAEQAANPRLPSVSTHWSL
                     RRCLDSLEEGAAGGGQAEQAAFAGQGLGARLALHPREGAPNLGALAELPPPLTLAIGS
                     ERGWTEGEVDLLEERGFRMAGLGRRILKTETAAVAAVALLLARLGRM"
     gene            complement(5609..6319)
                     /locus_tag="PLG14_07785"
     CDS             complement(5609..6319)
                     /locus_tag="PLG14_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOX49076.1"
                     /translation="MRKIGIVFAIEREARSTLDDSDFGWKAVGPGLYSSGRWPLALAV
                     SGIGKAFASCAAARVGQDASLLLSLGTSGSLLGPASVGSLLLAGEFVEYDIEAGALHY
                     PPGVTPLVGMRDPVIVSATEASLGPARAALSSLGLAHSEGRVASGDSFVDDPTRARFV
                     RERTGADAVDMESAALAKLAVYAGLGPDGAALECLALRVLSDCADRRAPRSWCEDGAS
                     LCACFPGLLKAFAPLYCG"
     gene            complement(6522..>7146)
                     /locus_tag="PLG14_07790"
     CDS             complement(6522..>7146)
                     /locus_tag="PLG14_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOX49077.1"
                     /translation="VSGRWPLRLALSGIGKAFAAFTAARASRGASLLLSLGTSGGLGG
                     SEEIGGLVLAAEFVEHDMEAGALHFAPGVTPYSEMRDPVMRTAGEATLGLARRALGAA
                     GLTFREGRVASGDSFIDDPSRARLLRERTGADAVDMESAAVAKIARYRLKDPEGAPLE
                     ALALRALSDAADRRAGRSWAELVDGQGLLLSRFLKAFAPLYCGSDAS"
BASE COUNT         1102 a         2660 c         2468 g          916 t
ORIGIN      
        1 tcgccgccgg gcgctcagag ccgcggcgcg gcggaagcaa gcatcaaggg ggctgcccac
       61 aggggcagcc ccctttttcg tgccaaaggc cgggcccggg cccggccttt ttgcgctcct
      121 ttcgccgaca tgtttgacac ttccaaccgc cgtgtcatat tgacactttt cctggaatgc
      181 gtattattga acgtgacatt gcccgcaggg atgtcatact ggagggaaaa tggcggttct
      241 catccgatca atcggaaccc acgtcccgga gcgcagggtc gcgaacgccg atctcgcggc
      301 gatggtggac acgacggacg aatggatcgt ctcgcacacg ggcatcaagg cgaggcactt
      361 cgcggccgac ggcatgctca cgagcgacat ctccgcggag gcggcccgcg tcgccctgga
      421 gagggccggt ctcggcccca aggacatcga cctcctcatc gtcgccacgg cgacgcccga
      481 ctacttcggc ttcccctcga cggcctgcat cgtccaggac aagatcggcg cctacggctg
      541 cgccgccttc gacctgggcg cgggctgcac gggcttcatc tacgccctgg acgccgcggc
      601 ggccatgctc gaggcccgca agggccgcaa cgccctggtc atcggcgcgg agaccctctc
      661 gcgcatcgtc gactggaagg acagggccac ctgcgtcctc ttcggggacg gggccggagc
      721 cgccgtcctc tcgcgcatcg acgagaccgg ccgaggcctc atcgcgagcg tcctgggcgc
      781 cgagggctcg ggccacaagg acctctacct cgtccaggag gagcgcgcga agaccttcga
      841 gcgctccgag cccatcgtgc ccgcgatcgc gatgaacggc cagaacgtct acaacttcgc
      901 cgtgaagacc atcaccgtcc tcgtctcgag gctcctccac cagaccgtct acaagctgga
      961 ggacttcgcc tggatcgtgc cgcaccaggc caacgcccgc atcgtgcagg cggcggccaa
     1021 gcgcctcaag gcccccgagg agaagttcta cctcaacatg gaggagtacg cgaacacctc
     1081 ggcggcgagc atacccctcg ccctggacga gatggaccgc aagggcctcc tcaagaaggg
     1141 cgacctcatc atgctgatcg gtttcggggc cggcctcacc tacggggccc aggccatccg
     1201 ctggtagacc ggccttcggc cttacgcggc aggccagggg cggaaggccc cccagccgcg
     1261 cggccgaggc gcgccccccc aaggagaata cgatgaacaa gcgcgtagcc atcaccggcc
     1321 tcggcgtcgt gagccccgtc ggcaacgacg tcccggcctt ctgggactcc atcaaggccg
     1381 gccgctccgg cgccgggacc gtgacccggt tcgacccctc gcgcctcgag tcccgcgtgg
     1441 cctgcgaggt caagggcttc gacccctccc tctacatgga caagaaggag gccaggaaga
     1501 tggccctctt cagccagttc gccgtggccg cggccgtcca ggcctggcgc gacggcggac
     1561 tgcccgagcg ttcccaggcc gaggagccgg gctcggggcc cgcctccgtc tccggcatgc
     1621 gggggcccta ccccttcgac cggatcggcg ccgtcatcgg caacggcatc ggcggcatcg
     1681 acgtctgcct cgagtcccac aagaagctcc tggagtccgg ccccgggcgc atgctcccga
     1741 tgaccgtgcc cctcatgatc gcgaacgagg cggcgggcaa cgtcgcgatg cgcctcggga
     1801 tcggcggccc ggccctcacc gcggtgacgg cctgcgcctc cggctccgac gccatcggcc
     1861 aggccctgga cctcatccgg gccggccgct gcgacgccat cctcgcgggc ggcaccgagg
     1921 ccgcgatcac cgagttcgcg atgggcggct tctgccgcct ccaggccctc tccaccgcct
     1981 acaacgacag tcccgagaag gccagccgcc ccttcgacaa ggcccgcgac ggcttcgtga
     2041 tcggcgaggg cgcagccgtc ctcctcctcg aggactggga caaggcggtc gcccgcggcg
     2101 cccggatcta cgctgagctc gctggctacg gcgcgagctg cgacgcctac cacctcaccg
     2161 cccccgaccc cggggccgag ggctgcgccc gcgcgatgcg cgccgccctg gccgacgccg
     2221 ggatgaggcc cgaggagatc gcctactaca acgcccacgg cacctccacg gcgatcaacg
     2281 accccgtgga gaccctcatg ctcaagaagg ccttcggcga gcacgcgaag cgcctgaagg
     2341 tctcctccac gaagagcatg acgggccact gcatcgccgc ggcgggcgcc atcgaggccc
     2401 tggtctgcgt gaaggccgtg gaggagggct tcgccccccc gaccatcaac ctggagaatc
     2461 ccgacctgga ggccggctgc gaccttgact atgtcccgaa caagggcgtg gccatgccca
     2521 tcgaggcggc ggcctcgggc tccctcggct tcggcggcca caacgcgatc ctcgtcgtga
     2581 ggaaggcccg caaatgatcg tcgacaccgt ccccgggaag gccctcaggg ccgagataga
     2641 aggctacctc ccccaccgcg cccccttcgc actcgtcgac gaggttgagg tctccgaggg
     2701 ccacatccgc ggcgccatgg tcttccccgc cgactccttc ttcttcgcgg gccacttccc
     2761 cggctacccc gtcgtgcccg gcgtcctcct cgtcgagtcg atggcccagt gcggcggcgc
     2821 cggcgtgaag cgcatgggcc tcgtcccgaa cggcaccttc ttcctcgcga aggtcaagga
     2881 ggcgcgcttc cgccgccagg tgcggcccgg ggagcgcttc cacatggaga tcgacaacat
     2941 caaggccacg ccgaacatcg tccaccagcg cggcgtcggc tacgtcgacg gcgagcccgc
     3001 cgtcgaggcc gagtggctct gcatagccgg agggaacgtc gcatgatcca gaagcccatg
     3061 gtccgctcca acatctgcat caacgcgcac cccctgggct gcgagcggga gaccctcgcc
     3121 caggtcgagt acgtcaaggc gcgccgctcc ctctacgaag gccgcggcgg gcccaaggcc
     3181 gtcctggtcg tgggcgcctc cacgggctac ggcctagcgt cgcgcatcgc ggccgccttc
     3241 ggcttccgcg cggcgaccgt cgggatctcc ttcgagaagg agccctcggc gaccaagccc
     3301 ggcacgcccg gctggtacga caaccggacc ttcgacgccg cggcccgggc cgagggcctc
     3361 ccctcgcgga gcctggacgg cgacgccttc tccaaggaga tgaaggagcg ggcggtccag
     3421 gcagcccgcg agctcgacgt gaagtacgac ctcgtggtct actccctcgc ctcgccggtc
     3481 aggaccgacc ccgccacggg cgaacttttc aagtccgcca tcaagcccct gggaaagacc
     3541 tacacgggcc gcaccgtgga cgtcttctcg ggcaagatct ccgaggcctc gatccagccg
     3601 gcgaccgagg aggaggccgc ctccaccgtg aaggtgatgg gcggcgagga ctgggccctc
     3661 tggatcgagg ccctgcgcga gggcggcgtc ctggcccccg gcgcgaccac cgtcgcctac
     3721 tcctatgtcg gcccctccct ctcctggccg atctaccgcg acggcacgat aggccgcgcc
     3781 aaggcccacc tggaggccac ggcgaaggag ctctccgcgc gttacgcgaa ggaggggctc
     3841 gcggcctacg tctccatcaa caaggccgtc gtcaccaggg cgagcgccgt catccccgtc
     3901 atccccctct atgtctccac cctcttcaag gtcatgaagg agaaggggag ccacgaggac
     3961 tgcgtcgcgc agatcgaccg gctctaccgg gaccgcctct acgcggcggc cccctcaggc
     4021 gcgcgccgcg tcccgcccct cgacgccgag gggcggatcc gcatcgacga cctggagatg
     4081 tccccgggcg tccaggcgga ggtcgaggct aggatggccc acgtcacgga ggaaaacctc
     4141 cgcgacctga ccgacgtcga gggcttcagg ctcgacttcc tacggatcca cggcttcgag
     4201 gtgcccggcg tcgactacga ggccgaggtg gcgcccgact acgatccggc cgggcccgcg
     4261 gcgtccggcg cggcgaggta gggccacagc ggaaatgccg acaccgaaaa ccaaggcgcg
     4321 ggaaggcggg cctcgcgagg agggacacgg ctcctagcag gcacagacga tatgccgttt
     4381 tcgtctccct tcccgagcaa gtcgagcgcc cgagcaagcc ttcccgcgcg cggcgttcca
     4441 atacgatgtt cccagggcgc gccgttcacc cggcgcgccc ttttctttgc gcgcccgcga
     4501 ccggacgcac cgcggccagg cgcgccgcgg tcggatggcg ccgaggacgg ccgcggcgtc
     4561 gagggcgccc ccgcgggcgg ccgcggccac tttactcggg aaccctcgcc gcgctagcct
     4621 cgagtccgtg aacatgctca tcctcgagcc gggcgagccc ggcgccctca tcccgcggag
     4681 cgaccggcgc tgcgagcacg tgaggaagat cctcaagaag aagccggggg accgcctcct
     4741 cgcgggcatc gccacggact cgcccttcgg gccggcgcct ggcgccctgg gcgaggccgt
     4801 cgtggaatcc ctcgacgaga ggggcctcgt cctctccttc gagcccttcc ccggcccgcg
     4861 ctcggagccc gaaggcctcg cgcccctccg ccttatcctc ggcttccccc gccccatcca
     4921 ggccaaccgg atcttcaagg acctcgcgag cctgggcgtc gcggagatcg tcctcacggg
     4981 caccgagctc ggcgagaagt cctacctgga aagcgacttt ttcagggctg gcgaattccg
     5041 gcgccccctc ctggaggggg ccgagcaggc ggcgaatccc cgcctgccct ccgtctctac
     5101 ccactggagc ttgcgtcgct gcctcgactc cctggaggag ggagccgcag gtggcgggca
     5161 ggcggaacag gccgccttcg ccggccaggg actgggcgcc cgcctggccc tccaccctcg
     5221 cgaaggcgcg cccaacctgg gcgccctcgc cgagcttccg ccgcccctca ccctcgccat
     5281 cgggagcgag cggggctgga ccgagggcga agtcgacctc ctcgaggagc ggggcttcag
     5341 gatggctggc ctgggccgaa ggatcctcaa gacggagacg gccgcggtcg ccgccgtcgc
     5401 cctcctcctt gcccgcctgg gcaggatgta ggccgaggcg ggagaggagg cccgcgcggc
     5461 cagcccgccg cgcggccgga agcggcgcgc ggcggaatca ggcgcgcaac cgagagcggc
     5521 gcgcgccggc agcccgcatc gcgccgcggg cgcgccgagc cttccgcgcc gcccttggac
     5581 agggccgcgc ggccgggcct cgccgccttc agccgcagta gagcggagcg aaggccttga
     5641 ggaggccggg gaagcaggcg cagagcgagg ccccgtcctc gcaccaggag cggggagccc
     5701 ggcggtcggc gcagtcgctg aggacgcgga gcgcgaggca ctcgagggca gcgccgtccg
     5761 ggccgaggcc cgcgtagacg gcgagcttcg cgagggcggc gctctccatg tccaccgcgt
     5821 cggctccggt cctctcgcgc acgaagcggg ccctggtcgg gtcgtcgacg aaggagtcgc
     5881 ccgaggccac cctgccttcc gaatgggcca ggccgaggga ggaaagggcg gccctggcgg
     5941 ggcccaggct cgcctcggtg gcggagacga tcacgggatc gcgcatcccc acgaggggcg
     6001 tcaccccggg cggataatgc agggccccgg cttcgatgtc gtactcgacg aactctccgg
     6061 cgaggaggag gctcccgacc gaggccgggc cgagaaggga gcccgaggtg cccagggaga
     6121 ggaggaggga ggcgtcctgg cccaccctgg ccgcggcgca ggaggcgaag gccttgccga
     6181 tacccgagac ggccaaggcg agcggccatc gccccgagga gtagagtccc ggcccgacgg
     6241 ccttccagcc gaagtccgag tcgtcgagcg tggaccgcgc ctcccgctcg atcgcgaaga
     6301 ctatcccgat cttccgcatg tcgccacctc gggctacagt ctcgtccagg cgggggccga
     6361 atgcaagctc gcaggcgagg agcgcggacg cgcgcgccgg aatcaggcgc gcgccggccg
     6421 cccgcctcgc gcagcgggcg cgccaggctt tccgcgcggc ctatacaggg gccgcgcggc
     6481 cgggcagcgc ggccgaggcg gcgccgccgg gaagccgggc cttaggaggc gtccgagccg
     6541 cagtagagag gcgcgaaggc cttgaggaag cgcgagagga gaaggccctg cccgtcgacg
     6601 agctcggccc aggagcggcc ggcgcgccgg tccgcggcgt cgctcagggc ccgcagggcc
     6661 agggcctcca ggggcgcgcc ctcggggtcc ttgaggcggt agcgcgcgat cttggcgacg
     6721 gcggcgctct ccatgtccac cgcgtccgcg cccgtcctct cgcggaggag ccttgcgcgg
     6781 gagggatcgt cgataaagga gtcccccgag gcgacgcggc cttccctgaa ggtgaggccg
     6841 gccgccccga gggcgcgccg ggccaggccg agcgtggcct cgccggcggt ccgcatcacg
     6901 ggatcgcgca tctcggaata gggggtcacg ccgggcgcga aatggagggc tccggcctcc
     6961 atgtcgtgct cgacgaattc ggcagcgagg acgaggccgc cgatctcctc ggagccgccg
     7021 aggccgcccg aggtgcccag ggagaggagg agggaggccc cccgggaagc cctggccgcc
     7081 gtaaaggcgg cgaaggcctt cccgatgccg gagagggcga gcctcagggg ccagcggccc
     7141 gagacc
//
LOCUS       DAOOSJ010000139         7128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6545_length_7128_cov_4.605259, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000139 DAOOSJ010000000
VERSION     DAOOSJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6545_length_7128_cov_4.605259"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..609)
                     /gene="recR"
                     /locus_tag="PLG14_07795"
     CDS             complement(22..609)
                     /gene="recR"
                     /locus_tag="PLG14_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOX49078.1"
                     /translation="MKALEELVGLLTRLPGIGRKSALRVAYHLLKTDQAYADALADRI
                     RGLKEAIRFCSVCGSYAESDPCPVCSSPGRDRSQICVVEQPQDVVTIEASHEYRGLYH
                     VLGGLISPLDGVGPEDLRLDALAARIRGGEAREIVVATNPTVEGDTTALYLKRALEPL
                     GVSVTRLATGIPVGGDLEYADRLTLARSFRGRVKL"
     gene            complement(606..941)
                     /locus_tag="PLG14_07800"
     CDS             complement(606..941)
                     /locus_tag="PLG14_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOX49079.1"
                     /translation="MNINDIFEMLKNPQAIQAQAEELKRRTASIEATGSSGGGLVRIT
                     LTGTLDMKSCSIAPEAVDPSDIGLLEDLVRAAYNDAAAKVREEVQRSLSSGLGGLPIP
                     PGLFGGGGL"
     gene            complement(931..3195)
                     /gene="dnaX"
                     /locus_tag="PLG14_07805"
     CDS             complement(931..3195)
                     /gene="dnaX"
                     /locus_tag="PLG14_07805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOX49080.1"
                     /translation="MPYEITAARKRPRGFEELAGQDFVSATLEASLESSRIAHAYLFS
                     GPRGCGKTSTARILAKALNCEQGPTSRPCGTCSSCRSIQAGSSLDVIEIDGASNTSVD
                     NVRQIKDEVLFPPNGGRYKVYIIDEVHMLSMSAFNALLKTIEEPPPYVVFIFATTELH
                     KVPATIKSRCQQFAFRLVPVEILKGLLQAAAAELGVKADDDALLWIAKEASGSVRDAY
                     TLFDQVLSFSEGHVTAAKIRDKLGLVGLDSMNALFEACVAGDASAPAGAAGSPAGPQA
                     GSVAGRALEALDAILSRGVSPEQALVDSVEYCRSLLLLKNGIARESLLGAAPAAYSPA
                     VLEAFSSEQIERALAGLLELHRNIRFTVDPRFELELAVSRLCRLRDYVAPSELARVVS
                     GLRRRLAEGGSQQGGSALGGASPGGRPSGRGLQPEPRARAEDGGRLSDDDGLEGGGPA
                     EPEKKKPELPTSLKSAFSSLAAAAAAAEPEAPVAPMRRPMAAPVARAAQDSGREAPRA
                     AVAAAQAAPGAPAGPMTAPAAPGPQAAAPAPARQPIAPPPPDRDGVAEDEEEDPEGGE
                     TWVPEPAQAEAAGQVPAAGDGVAGLSVSPNAQGAGAGASPRAARPAAGAPEAAPPAGL
                     DPEELKRLVIESLGRSSPLLKSGLSASLRWRIEDERVVVPFRSGMEESLLKGELGVVS
                     RAVAQAAGRAYKVELRVEPDRPRGREGQAPSGAAGPEGPGGEEAAGDPVAVVERVFRG
                     TRVKERESGGRGEY"
     gene            complement(3257..3354)
                     /gene="ffs"
                     /locus_tag="PLG14_07810"
     ncRNA           complement(3257..3354)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG14_07810"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3381..3466)
                     /locus_tag="PLG14_07815"
     tRNA            complement(3381..3466)
                     /locus_tag="PLG14_07815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3430..3432),aa:Ser,seq:gga)
     gene            complement(3494..3580)
                     /locus_tag="PLG14_07820"
     tRNA            complement(3494..3580)
                     /locus_tag="PLG14_07820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3544..3546),aa:Ser,seq:cga)
     gene            complement(3589..3662)
                     /locus_tag="PLG14_07825"
     tRNA            complement(3589..3662)
                     /locus_tag="PLG14_07825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3626..3628),aa:Arg,seq:gcg)
     gene            complement(3672..3759)
                     /locus_tag="PLG14_07830"
     tRNA            complement(3672..3759)
                     /locus_tag="PLG14_07830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3723..3725),aa:Ser,seq:gct)
     gene            complement(3774..3858)
                     /locus_tag="PLG14_07835"
     tRNA            complement(3774..3858)
                     /locus_tag="PLG14_07835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3822..3824),aa:Ser,seq:tga)
     gene            complement(3977..4468)
                     /locus_tag="PLG14_07840"
     CDS             complement(3977..4468)
                     /locus_tag="PLG14_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49081.1"
                     /translation="MQLKNPEIISAIESMGEHYRSNISNRFTRRALSSMTLDPGTWNL
                     IEDFTEKSENYRYQGYHLDELYSQILAMARFVYQARKQIGPSLRYLAQSSGGSERFTD
                     SDRILRDMAVNNFGSNLKILADRLNELYVKVAAIDKEAAGQKSPVYSQIAELREIGRY
                     LVE"
     gene            4547..5824
                     /locus_tag="PLG14_07845"
     CDS             4547..5824
                     /locus_tag="PLG14_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49082.1"
                     /translation="MKRKKSIVVAAAAGLALAAVALSLALSFLSAAKEGPAAVWTDVP
                     ELALYAELFNASQGRYRVEVEWKADLPSSIRDSKAQPALAVGRYLKSQAVRDRFQSLD
                     YLFGELLVNQAAFYPVLLSLGNVEGRQLLLPLSFNLPTIVFAAGGEAKAADDFTIGVE
                     ELKAAAAYNRKKGSSYDRMGFSPRWDGEFLTTLVNSGGASFKEGRPLAWSDQGLSAAV
                     ERARAWSESANGSRSAEEDFQFKYLYTPAYKYVAEGRALYAYLKSSDFFLVPEEKRSL
                     LDYRWFAEGGAVPVDEAIVFAAMPRAGKGKAAAEAFLKWLYKEDSQKAALESARKTRS
                     LEGSFGVAGGFSALRSVNERVFPLFYPALVGHLPPADGLSAPNVLPSDWPAIKEKVLK
                     PWLLEATGRPPSARSPGQELQARLAEYLKNSGR"
     gene            complement(5964..6995)
                     /gene="dusB"
                     /locus_tag="PLG14_07850"
     CDS             complement(5964..6995)
                     /gene="dusB"
                     /locus_tag="PLG14_07850"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOX49083.1"
                     /translation="MSDNASLHRPVIVGGAAIPGNLFLAPVAGYSDAAFRSVCADEGA
                     DLCFTEMVSAEALVRGHKRTRELMARAENETRYAIQLFGSKPETLARAAILCLEFKPA
                     LVDLNCGCPVPKIVKAGAGSALMREPGLVREIVRAMAEALSPSGVPVTVKIRSGWDEG
                     SRNFMEVAAAAVEGGAAAVTLHARTRAMGYSGKAAWPEIARLAAALPVPVFGSGDLFS
                     AEAARRMLEETACAGAMIARGAMGNPFVFREARALLEGLPAPAIGVREKAEAARKHLL
                     LSVRFLGERTACVEFRKQFCSYTKGTVAGAALRSEAVRAAGLAELLALLDRWAASGDP
                     AFGESASEA"
BASE COUNT         1023 a         2504 c         2564 g         1037 t
ORIGIN      
        1 ccttggcgcc cagccccggt cctacagctt cactcgtccg cggaaggagc gggccagcgt
       61 cagccggtcc gcgtactcca ggtcgccgcc cacggggatg cccgtcgcaa gccgcgtcac
      121 ggagacgccc aggggctcta gggcgcgctt caggtagaga gcggtcgtgt ctccctccac
      181 cgtcggattg gtcgcgacga cgatctcccg cgcctccccg ccgcggatcc gcgcggccag
      241 ggcgtcgagc ctgaggtcct cggggccgac gccgtccagc ggcgagatga gcccgcccag
      301 gacgtggtag aggccgcggt actcgtggga agcctctatc gtcacgacgt cctgcggctg
      361 ctccacgacg cagatctgcg agcggtcgcg gccgggcgag gagcagacgg ggcaggggtc
      421 ggactcggcg taggagccgc agacggagca gaagcggatg gcctccttga ggccgcggat
      481 ccggtccgcg agggcgtcgg cgtaggcctg gtccgtcttg aggaggtggt aggccacgcg
      541 gagcgcgctc ttccggccga tgcccggaag gcgcgtgagg aggccgacga gctcctccag
      601 ggccttcaca ggccgcctcc gccgaagagc ccgggcggga tgggcaggcc gccgagcccc
      661 gaggagaggg agcgctggac ctcctcgcgg accttggctg ccgcgtcgtt gtaggcggcc
      721 cgcaccaggt cctcgagaag gcctatgtcg gaggggtcga cggcctccgg cgcgatcgag
      781 caggacttca tgtccagggt cccggtcagc gttatgcgaa ccagcccgcc gcccgagctg
      841 cccgtggcct cgatggaggc cgtccggcgc ttgagctcct cggcctgggc ctggatggcc
      901 tgggggttct tgagcatctc gaagatgtcg ttaatattca ccgcgtcctc cgctttccct
      961 ttccttcacc cgagtccccc tgaagacgcg ctccacgacg gccacgggat cccccgccgc
     1021 ctcctcgccg cccgggccct ccgggccggc cgcgccggag ggagcctggc cctcgcggcc
     1081 gcggggccgg tccggctcca cgcggagctc gaccttgtag gccctgcccg cggcctgggc
     1141 gacggcccgc gacacgaccc cgagctcccc cttgaggagg ctctcctcca tcccgctccg
     1201 gaaggggacg acgacgcgct cgtcctcgat cctccagcgg agggaggccg agaggccgct
     1261 cttgaggagg gggctcgagc ggcccaggga ctcgatgacg aggcgcttga gctcctccgg
     1321 gtcgaggccg gccggcggcg cggcctcggg agcgccggcc gcgggccgcg cggcccgcgg
     1381 ggacgcgcct gcgcctgcgc cttgcgcgtt gggcgacacc gaaaggcccg ccacgccgtc
     1441 gccggccgcg ggaacctggc ccgccgcctc ggcctgggcc ggctcgggga cccaggtctc
     1501 gccgccctcg gggtcctcct cctcgtcctc cgcgacgccg tcgcggtcgg gcggaggcgg
     1561 ggcaatgggc tgccgcgcgg gggccggggc cgccgcctgc ggaccagggg cagcgggcgc
     1621 cgtcatcggg cccgccggcg ccccgggggc cgcctgagcg gcggcgacgg ccgctctcgg
     1681 cgcttcgcgc cctgagtcct gggccgccct tgcgacgggc gcggccatgg ggcggcgcat
     1741 cggcgccacg ggagcctcgg gctcggcggc ggcggccgcg gcggccaggc tcgagaaggc
     1801 ggacttcagg ctggtcggaa gctcaggctt ttttttttcg ggctcggcgg gtccgccgcc
     1861 ctccaggccg tcgtcgtcgg agaggcggcc gccgtcctcg gccctcgccc gcggctcggg
     1921 ctgaaggccg cggcccgatg gccggccgcc cggcgaagcg ccgcctaggg cggagccgcc
     1981 ctgctgcgag cccccctcgg cgaggcgccg cctgaggcct gagacgacgc gggcgagctc
     2041 ggagggggcg acgtagtcgc ggaggcggca gagccgcgag accgcgagct cgagctcgaa
     2101 gcgcgggtcg acggtgaagc ggatgttccg gtggagctca agcaggcccg cgagggcgcg
     2161 ctcgatctgc tcggaggaga aggcctcgag gacggcgggg ctgtaggccg cgggcgcggc
     2221 gcccaggagg gactcgcggg cgatgccgtt cttgaggagg aggagggagc ggcagtactc
     2281 gaccgagtcg acgagggcct gctcggggct cactccccgc gaaaggatgg cgtccagggc
     2341 ctcgagggcc ctgccggcga cgcttcccgc ctggggcccc gcgggcgatc ccgccgcgcc
     2401 ggccggcgcg gacgcgtccc cggccacgca ggcctcgaag agggcgttca tcgagtccag
     2461 gcccacgagg cccagcttgt cgcggatctt cgcggccgtg acgtggccct cggagaagga
     2521 gagcacctgg tcgaagagcg tgtaggcgtc gcggaccgag cccgaggcct ccttggcgat
     2581 ccagaggagg gcgtcgtcgt cggccttgac gccgagctcg gccgccgccg cctggaggag
     2641 gcccttgagg atctccacgg ggacgaggcg gaaagcgaac tgctggcagc ggctcttgat
     2701 cgtggccggc accttgtgca gctcggtggt ggcgaagatg aagacgacgt agggcggcgg
     2761 ctcctcgatg gtcttgagga gcgcgttgaa ggcgctcatc gagagcatgt ggacctcgtc
     2821 gatgatgtag accttgtagc ggccgccgtt cggggggaag aggacctcgt ccttgatctg
     2881 ccggacgttg tcgacggagg tgttggaggc gccgtcgatc tcgatcacgt cgagcgagga
     2941 gcccgcctgg atcgagcggc aggaggagca ggtcccgcag ggccgcgagg tcggcccctg
     3001 ctcgcagttg agcgccttcg cgaggatgcg cgcggtgctg gtcttcccgc agccgcgggg
     3061 gccggagaag aggtaggcgt gggcgatgcg ggagctctcg agggaggcct cgagggtggc
     3121 ggacacgaag tcctggccgg cgagctcctc gaagccccgg gggcgcttcc tggccgcggt
     3181 gatctcgtaa ggcatggggg gatagtagcg caaagagggg gcggggtaaa gcgggagcct
     3241 aaaagcatcg agcgtccgaa gccaggcggt ctgcggcacc gcatcttccg cttaccgttg
     3301 cttccttccg gacctggcgg ggttgggcgg cttccgcgtg cgcagtgcct ggcttccgac
     3361 gctcgaaggg gatcgatttg cggagagaga gggattcgaa ccctcggtgc cttgtgagca
     3421 cgcacgcttt ccaagcgtgg accttaaacc actcggacat ctctccaata agaaggcgct
     3481 tttcaatgcg aaccggagag agagggattc gaaccctcgg aacccttacg ggttcaacgg
     3541 ttttcgagac cgtccgattc aaccgctctc gcatctctcc aagaaacatg aggcaaagag
     3601 gattcgaacc tccgaccttc agatccgcaa tctgatgctc tatccagctg agctattgcc
     3661 tcgagaaaat ccggagaggg ggggattcga acccccggta cccttttggg gtacaactcc
     3721 ttagcaggga gcccgattcg gccactctcg cacctctcca aaccgtccac atccggagcg
     3781 agtgggattc gaacccacgg cgcctttcgg cgcaacggtt ttcaagaccg tcaccataga
     3841 ccactcggac accgctccag cggtcgtgga accacacttt acggaaaagc gaaaggcttg
     3901 tcaataccgg cggacttccg gacaccggga ccttgccata cccctttcgc tcgaaccgaa
     3961 aggggttccc gccccgctat tccactaggt agcggcctat ctcccggagc tcggcgatct
     4021 ggctgtagac gggggacttc tgccccgccg cctccttgtc gatcgccgcg accttcacgt
     4081 agagctcgtt caggcggtcg gccaggatct tcaggttgga gccgaagttg ttgaccgcca
     4141 tgtcgcgcag gatccggtcg gagtcggtga agcgctccga gccgccggag gactgggcca
     4201 ggtagcgcag gctcggtccg atctgcttgc gggcctggta gacgaagcgg gccatggcca
     4261 ggatctgcga gtagagctcg tccaggtggt agccctggta gcggtagttc tccgacttct
     4321 ccgtgaagtc ctcgatcagg ttccaggtcc ccggatccag ggtcatggag gagagggccc
     4381 gcctggtgaa gcggttcgag atgttcgatc tatagtgctc gcccatcgac tcgatggcgg
     4441 agatgatctc ggggttcttc agttgcatgg cggaggcagt ataccccata gcgcgagccc
     4501 tgtcaccgcc ttgcgcgggc gggccggagc gggctatcgt cggggcatga agcgcaagaa
     4561 gagcatcgtc gtcgcggccg cggccggcct ggccctggcg gccgtggccc tgtcgctggc
     4621 gctctccttc ctctccgcgg ccaaggaagg ccccgccgcc gtgtggacgg acgtgcccga
     4681 gctggccctc tacgccgagc tcttcaacgc ctcccagggg cgctaccgcg tggaggtcga
     4741 gtggaaggcg gacctcccct cctccatccg ggactccaag gcccagccgg ccctggccgt
     4801 gggccgctac ctgaagagcc aggccgtgcg cgaccgcttc cagtccctcg actacctctt
     4861 cggcgagctc ctcgtgaacc aggccgcctt ctaccccgtc ctcctctccc tcgggaacgt
     4921 ggagggccgc cagctcctcc tccccctctc cttcaacctg ccgaccatcg tcttcgcggc
     4981 gggaggcgaa gccaaggcgg cggacgactt cacgatcggg gtcgaggagc tgaaggcggc
     5041 cgcggcctac aaccggaaga agggctcctc ctacgaccgc atgggcttct cgccccgctg
     5101 ggacggggag ttcctgacga cgctcgtcaa ctccggcggc gcctccttca aggagggaag
     5161 gcccctcgcc tggagcgacc agggcctctc ggcggcggtc gagcgcgcca gggcctggtc
     5221 cgagtcggcc aacggctcca ggtcagcgga ggaggacttc cagttcaagt acctctacac
     5281 gccggcctac aagtacgtcg cggaggggcg cgccctctac gcctacctca agtcctccga
     5341 cttcttcctc gtgccggagg agaagcgctc cctcctcgac taccgctggt tcgccgaagg
     5401 cggagcggtg cccgtggacg aggccatcgt cttcgcggcc atgccgcgcg ccggcaaggg
     5461 caaggccgcc gccgaggcct tcctcaagtg gctctacaag gaggacagcc agaaggccgc
     5521 cctggagagc gcgaggaaaa cccgctccct ggagggctcc ttcggcgtcg cgggcggctt
     5581 ctccgccctg cggtcggtga acgagcgggt cttccccctc ttctaccccg ccctggtcgg
     5641 ccacctgccg cccgccgacg gcctctcggc ccccaacgtc ctcccgagcg actggcccgc
     5701 catcaaggag aaggtcctca agccctggct cctcgaggcg acgggccgcc ccccgagcgc
     5761 ccgctccccc ggccaggagc tccaggcccg cctcgccgag tacctcaaga actcggggcg
     5821 ctaggctcgg cgctcgcggc gcggcggcct tcgccgcgca aaccgccgcg cgaaaggcca
     5881 gccccgcgag ccgccgcgcg ccgccgccgc gccatcccgc aggcgccgcc cgcgggccgg
     5941 cgcctgtccg gaaaggcgcg cgctcaggcc tcggaggcgc tctcgccgaa ggcggggtcg
     6001 cccgaggcgg cccagcggtc caggagggcc aggagctcgg ccaggcccgc ggcgcggacg
     6061 gcctccgacc tcagggccgc gcccgcgacc gtccccttcg tgtaggagca gaactgcttc
     6121 cggaactcca cgcaggccgt gcgttccccc aggaagcgga cggagaggag gaggtgcttg
     6181 cgcgccgcct cggccttctc gcggaccccg atcgccgggg cgggcaggcc ctccaggagg
     6241 gcccgcgcct cgcggaagac gaagggattc cccatcgccc cccgcgcgat catcgcgccc
     6301 gcgcaggcgg tctcctcgag catccgcctc gccgcctccg cggagaagag gtcgcccgag
     6361 ccgaagaccg gcacgggaag ggccgctgcc aggcgggcga tctcgggcca ggcggccttc
     6421 ccggagtagc ccatcgcgcg ggtcctcgcg tggagggtca cggccgcggc cccgccctcc
     6481 acggcggcgg cggccacctc catgaagttg cggctgccct cgtcccagcc cgagcggatc
     6541 ttcaccgtga cggggacgcc ggagggagag agggcctcgg ccatcgcgcg gacgatctcg
     6601 cggaccaggc ccggctcccg catgagggcc gagcccgcgc cggccttgac gatcttcggc
     6661 acggggcagc cgcagttcag gtcgacgagg gccggcttga attccaggca aaggatggcg
     6721 gcgcgggcca gggtctccgg cttggagccg aagagctgga tcgcgtaacg cgtctcgttc
     6781 tcagccctgg ccatgagctc ccgcgtcctc ttgtgcccgc ggaccagggc ctcggcgctc
     6841 accatctccg tgaagcagag gtcggccccc tcgtccgcgc agaccgagcg gaaggccgcg
     6901 tcggagtagc ccgcgacggg cgcgaggaag aggttgcccg gaatggccgc gccgccgacg
     6961 atgacgggcc ggtgcaggga cgcgttgtcg ctcatggaag tctccgtaga cgggaaccgc
     7021 gaacggagcc tcttccaggc ttccgttcgc ggttcgatgt tcgtttcgtc ggcccttgaa
     7081 ggccctcgca gggggctgaa ggccccgggc cgagtccgcc gccggccc
//
LOCUS       DAOOSJ010000140         7077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6598_length_7077_cov_5.209057, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000140 DAOOSJ010000000
VERSION     DAOOSJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6598_length_7077_cov_5.209057"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLG14_07855"
     CDS             <1..193
                     /locus_tag="PLG14_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49084.1"
                     /translation="RAICVNKRGLSDAPWTLDPRLKSSKSFEFDFLRGGLSSRDDLTA
                     LDNSGLVADFMAEASKGTA"
     gene            190..834
                     /locus_tag="PLG14_07860"
     CDS             190..834
                     /locus_tag="PLG14_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49085.1"
                     /translation="MNPYLLSALVILALAATAQYYLGTKKNRVIAAAISKGAEEILKP
                     TMTNYVNIGGAIGHNFSYSLSGGAWTSAKGTMTLCPRHSLLYLPFSRLLGFGVDRFFI
                     NLFTKKKLRGEGHVLCASELKRAKIDGLAEMERREVEAAGKRFVLLWRGADLSGELEA
                     LLRAMPDPKRLRHFCSFADNKTFFLKTYPAAGNVRDDLKALLPRLDAFLDKAKE"
     gene            837..1115
                     /locus_tag="PLG14_07865"
     CDS             837..1115
                     /locus_tag="PLG14_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49086.1"
                     /translation="MELDAGTRGKLDAVLAKVKEPLSELSLQELGLVAKFTYSAKEKT
                     IVAVLNVDAARFECPACSAMYGEVIQGIKRNLTEELSKEFPGFTILFE"
     gene            complement(1317..1874)
                     /locus_tag="PLG14_07870"
     CDS             complement(1317..1874)
                     /locus_tag="PLG14_07870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOX49087.1"
                     /translation="MKYYRKIVGERVYLSPISLDDAERYALWLNDLSTTRYLTLAALN
                     VTLHGERDALANLSKGHNYAIVEKETDELLGNCGLMDIEQVNRTAEVGVFLGEESTRG
                     KGYGAEALRLLCDYSFNVLNLRSLMLRVYDYNERGMASYRKVGFKEMGRRRKAHFYGG
                     AYHDLVFMDLLAEELGPSVLPPALA"
     gene            complement(2082..3848)
                     /locus_tag="PLG14_07875"
     CDS             complement(2082..3848)
                     /locus_tag="PLG14_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOX49088.1"
                     /translation="MAQQIKLFSLSELRPALLRAFKGGYTGADLGKDLASGLTVGVVA
                     LPLAMAFAIGAGATPAQGLYTAIVAGFLIALLGGSKYQVSGPTGAFVVIIADVILRQG
                     MDGLLLATIVAGLLLVFLGVSGLGVLIKYIPYPVTIGFTTGIGTIIFVGQLKDLFGLA
                     IPSLSPEFFGKIGQYASYATTLSPWAALLGLGTIAIIVLVRKLAPRVPAAVTAIAVVT
                     VAAFALGLPVETIGSKYGAIPRGFPAPVLPAFSLEKLRAVFPSALTIAMLAAIESLLS
                     AVVADGMTGDRHGSSMELVAQGLGNIASALFGGIPATGAIARTATNIKAGARSPVSGI
                     IHAFVLLLFTLFLGPLASAIPLAALAGVLVVVASDMSELPRFFNMRRAPRSDLVVMLV
                     TFTLTVVIDLTAAVQVGVLLAIFLFIKRMSETTTLSQSQSEILAAIGGAEAAEEAGAP
                     VHAPAGCEVYEIDGPFFFGVADMLQDAERQFERPPEAFVLRMRKVPAIDATGLNALAS
                     FAKGARRQGTALILSEVQAQPRAALAKMGLDAEIGLENVTADLAGAFARAEVLAAARK
                     EAAAKRVAESGDRAEKPARTKI"
     gene            complement(3885..4526)
                     /locus_tag="PLG14_07880"
     CDS             complement(3885..4526)
                     /locus_tag="PLG14_07880"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HOX49089.1"
                     /translation="MPDVMPLGGLSERRPARPGRPTWSTRLGVLLAAGAFLATAALLA
                     AASLASCAGKARDPEFVPADYASWRKTTELRLDYPIPGHEDRLRVIRMNEAGFAVRPV
                     MLDGRLRYDFPAGAVIAKEIYQGSSPAPGEAPVSITAMVKAPGDPRALGGWLWIAKDL
                     SKPFPNETVFGDRFCVGCHANANERHPYGDRNKAEEFRDYVFFVPSAASPPIP"
     gene            complement(4519..5955)
                     /locus_tag="PLG14_07885"
     CDS             complement(4519..5955)
                     /locus_tag="PLG14_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX49090.1"
                     /translation="MARRGDSRGLSLTAKVNAIILVSLTAGVGAVVFAFALALVSSRD
                     SLTKAALGQQAAILATSVENFMLPGEAPVAVRFFAEVASVSPDTAVALYRRTGQRAFS
                     DGTTIARVNSNIGRERFLPRPGPEAPAEPPPRFAESASKPPKELFFRESEGGRSYYRS
                     LRPLVNYPKCTGCHGADHTLRGVIDIRVDVSPVARAQERTIAASGGGFLAVVALLALV
                     LGAYLRRVVLAPLVSIGRLCLDVTKGRFEGRVEVRSKDEIGELARTVNEMARGLYERF
                     ELTKYVSSGTIDALAAGQEPSRVARTLLFTDVRGFTSYTERKGPEEVVAVLNRLLEVQ
                     ARLIHEAGGDIDKFVGDEVVAVFEGEDSAERACAAALSIKAAVEADGAPFDGLRLGAG
                     IATGSVIRGMIGSERRADFTVIGDSVNVASRLCSMAKPGQVVACATTKRLAAGAFAFA
                     GPYGAKLKGKEETQRVYLLSGAKGGSDA"
     gene            complement(6011..6718)
                     /locus_tag="PLG14_07890"
     CDS             complement(6011..6718)
                     /locus_tag="PLG14_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOX49091.1"
                     /translation="MPIDDESVDPGLLRERVYGKLKDLLNAGRLKPGAFLDLTAIGRE
                     LGLSRTPLRDALIRLEIEGFVRIYPRRGVMVRSLDLQTIKNVYELIGALESAVIAGLK
                     GKYGPEDSARMAALNAEAGEALARDDFSAYYAANLEFHRVYLGLSDNAELVRSVNILK
                     ERLYDFPRRTGFVKEWEVASVREHGELIGRLDRGDFAGAAAFVREVHWSYESQERFIK
                     RYYSDGPGSRAPGGGED"
     gene            complement(6865..>7077)
                     /locus_tag="PLG14_07895"
     CDS             complement(6865..>7077)
                     /locus_tag="PLG14_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOX49092.1"
                     /translation="LPLSFDIAWYEQKAVIVLLALLSLGVKNIRLGPTLPAFLSPGVA
                     KVLVESFGIKGIVDPKADVAAMMSGK"
BASE COUNT         1096 a         2335 c         2627 g         1019 t
ORIGIN      
        1 gagggccatc tgcgtcaaca agcgcggcct ctccgacgcg ccctggaccc tggaccccag
       61 gctcaagtcc tccaagagct tcgaattcga cttcctgcgc gggggcctct cctcccgcga
      121 cgacctcaca gccctggaca actcaggcct cgtcgccgac ttcatggccg aagcctcgaa
      181 aggaacagca tgaatcccta tctcctgagc gccctcgtca tcctggccct cgccgcgacg
      241 gcgcagtact acctaggcac gaagaagaac cgcgtcatcg cggccgccat ctccaagggg
      301 gccgaggaga tcctgaagcc gacgatgacc aactacgtca acatcggcgg cgccatcggc
      361 cacaacttct cctactccct ctcaggcggc gcctggacga gcgcgaaggg cacgatgacc
      421 ctctgccccc gccattccct cctctacctc cccttctccc gcctcctggg cttcggcgtg
      481 gaccgtttct tcatcaacct cttcacgaag aagaagctcc gcggcgaggg ccatgtcctt
      541 tgcgcgagcg agctcaagcg ggccaagatc gacgggctgg cggagatgga gcgccgcgag
      601 gtggaggcgg cgggcaagcg cttcgtcctc ctctggaggg gcgccgacct ctcgggcgag
      661 ctcgaggccc tgctcagggc catgcccgac ccgaagcggc tcaggcactt ctgctccttc
      721 gccgacaaca agaccttctt cctcaagacc tatcccgcgg cgggcaatgt cagggacgac
      781 ctcaaggccc tgctgccgcg gctggacgcc ttcctggaca aggcgaagga gtgagcatgg
      841 aactggacgc gggaacccgg ggcaagctgg acgcggtgct cgcgaaggtg aaggagcccc
      901 tgtccgagct cagcctccag gagctcggcc tcgtggcgaa gttcacctac tcggccaagg
      961 agaagaccat cgtcgccgtc ctcaacgtgg acgccgcccg cttcgagtgt cccgcctgct
     1021 cggcgatgta cggcgaggtc atccagggca tcaagcgcaa cctgacggag gagctttcga
     1081 aggagttccc cggcttcacg atcctcttcg agtagccttg aggcttcggc ctcgccctgt
     1141 aacggagaac tcggccctcg cggccgggtt ttttgtcgca ggcgcgcgtc gccgatagcc
     1201 ggggccgcct gggcctgtgc ctcgcgaggc cgcgccgcct aagccggcgc cgcccgggcc
     1261 ggcgcgcgtc tccgatcgcc gggaccgctt ggccgcccgc gcccgggccg gcgggctcaa
     1321 gccagcgcgg gcggcaggac gctcggaccc agctcctccg cgaggaggtc catgaagacg
     1381 aggtcgtggt aggcgccccc gtagaagtgg gccttgcgcc tgcggcccat ctccttgaag
     1441 ccgaccttcc ggtaggaggc catgccgcgc tcgttgtagt cgtagaccct gagcatgagg
     1501 ctgcggagat tgaggacgtt gaaggagtag tcgcagagga ggcgcagggc ctcggcgccg
     1561 tagcccttgc cgcgggtgga ctcctcgccc aggaagacgc cgacctcggc cgtccggttc
     1621 acctgctcga tgtccatgag gccgcagttc cccaggagct cgtcggtctc cttctccacg
     1681 atggcgtagt tgtggccctt ggagaggttc gccagggcgt cgcgctcgcc gtggagggtc
     1741 acgttgagcg ccgcgagcgt gaggtagcgc gtcgtcgaga ggtcgttgag ccagagcgcg
     1801 tagcgctcgg cgtcgtcgag ggagatcggc gaaaggtaga ccctctcgcc gacgatcttc
     1861 ctgtagtact tcatggcggg cctcctccct gggagtatgg ccccgccagg gcgcgaaggc
     1921 aaggcgcgga cggccgctgg cggacgccgc tgacggcgcg gccggagcgg cgcggctcgg
     1981 gcggcgcgtc ccagaaagct cggcccccgc cgccgccgcg tgaggcgcgg cttggcggcg
     2041 aacgccgaag gcggctccgg aacggagcgg ccgcgcatcc tctagatctt cgtgcgggcg
     2101 ggcttctcgg cgcggtcgcc ggactcggcg acgcgcttgg ccgcggcttc cttccgcgcg
     2161 gcggccagga cctcggcgcg ggcgaaggcg ccggccaggt ccgccgtcac gttctccagg
     2221 cctatctccg cgtccagtcc catcttggcc agggcggcgc ggggctgggc ctgtacctcg
     2281 ctcaggatga gggccgtgcc ctggcgcctc gcccccttcg cgaagctcgc gagggcgttg
     2341 aggccggttg cgtctatcgc gggcaccttg cgcatccgca ggacgaaggc ctcgggcggc
     2401 cgctcgaact gcctctctgc gtcctggagc atgtccgcga cgccgaagaa gaagggcccg
     2461 tcgatctcgt agacctcgca gccggcgggg gcgtggaccg gggcgccggc ctcctccgcg
     2521 gcctcggcgc cgccgatggc cgcgaggatc tcggactggg actggctcag cgtcgtcgtc
     2581 tcgctcatcc gcttgatgaa gaggaagatg gccaggagga cgccgacctg gaccgcggcc
     2641 gtgaggtcga tcaccaccgt gagggtgaag gtgacgagca tgacgaccag gtcgctcctg
     2701 ggggcgcggc gcatgttgaa gaagcggggc agctcgctca tgtcggaggc gacgacgacc
     2761 aggacgcccg ccagggccgc gagcggaatc gcggaggcca ggggacccag gaagagggtg
     2821 aagaggagga ggacgaaggc gtggattatc cccgagaccg gggagcgggc gccggccttg
     2881 atgttggtcg cggtgcgcgc gatggcgccg gtggcgggga tgccgccgaa gagggcgctc
     2941 gcgatgttgc ccaggccctg ggcgacgagc tccatgctcg agccgtgccg gtcgcccgtc
     3001 atgccgtcgg cgacgacggc ggagaggagg gactcgatgg cggcgagcat cgcgatggtc
     3061 agggccgagg ggaagacggc gcggagcttc tccagggaga aggcgggcag gacgggcgcg
     3121 gggaagccgc gggggatggc gccgtacttg gagccgatcg tctccaccgg gaggccgagg
     3181 gcgaaggcgg cgaccgtgac cacggcgatg gccgtcaccg cggcgggcac gcggggggcg
     3241 agcttccgca cgaggacgat gatggcgatg gtgcccaagc cgaggagggc ggcccagggg
     3301 gacagggtcg tcgcgtagga ggcgtattgg ccaatcttgc cgaagaactc gggggagagg
     3361 gaggggatcg cgaggccgaa gaggtccttg agctggccca cgaagatgat cgtcccgatg
     3421 cccgtcgtga agccgatggt gacgggatag ggaatgtact tgatgaggac gcccaggccc
     3481 gacacgccca ggaacacgag gaggaggccc gcgacgatcg tcgcgaggag gaggccgtcc
     3541 atgccctgcc tgaggatcac gtcggcgatg atgacgacga aggcgcccgt gggccccgag
     3601 acctggtact tgctgccccc gaggagggcg atgaggaagc cggcgacgat ggccgtgtag
     3661 agtccctgcg cgggcgtggc gccggcgccg atggcgaagg ccatggcgag gggcagggcg
     3721 accacgccga cggtgaggcc ggaggcgagg tccttgccga ggtcggcgcc cgtatagccg
     3781 cccttgaagg cgcgcaggag ggcgggcctg agctccgaca gcgagaaaag cttgatttgt
     3841 tgtgccatta tggccaaagg tttagcgctt tcgcggggaa agggtcaagg gatgggcggg
     3901 gaggcggcgg acgggacgaa gaagacgtag tcccggaact cctcagcctt gttcctgtcc
     3961 ccgtaggggt ggcgctcgtt cgcgttcgcg tggcagccca cgcagaagcg gtcgccgaag
     4021 acggtctcgt tggggaaggg cttggagagg tccttcgcga tccagagcca gccgcccagg
     4081 gcccggggat cgcccggggc ctttaccatc gccgtgatgg agacgggagc ctccccgggg
     4141 gcgggcgagg agccttggta gatctccttc gcgatcacgg cgcctgcggg gaagtcgtag
     4201 cggagccgcc cgtccagcat gaccgggcgg acggcgaagc ccgcctcgtt catcctgatg
     4261 accctgaggc ggtcctcgtg gcccgggatg ggatagtcca ggcggagctc ggtggtcttg
     4321 cgccaggacg cgtagtccgc cgggacgaac tcgggatccc gggccttccc cgcgcaggag
     4381 gccaaggagg cggcggctag gagggcggcc gtggcgagga aagcgccggc ggcgaggagg
     4441 acgccgagcc gggtggacca ggtcggccgg cctggccggg ccggccggcg ctcagatagt
     4501 ccgcctaaag gcatgacgtc aggcatcgct tcctcccttg gcgcccgaga ggaggtagac
     4561 ccgctgggtc tcctccttgc ccttgagctt cgcgccgtag ggcccggcga aggcgaaggc
     4621 gcccgcggcg agccgtttcg tggtcgcgca ggcgacgacc tggccgggct tggccatgga
     4681 gcagagccgg ctcgcgacgt tgacggaatc gccgatcacc gtgaagtcgg cgcgccgttc
     4741 ggagccgatc atgccgcgga tcaccgagcc cgtcgcgatc ccggcgccga gcctgaggcc
     4801 gtcgaagggc gcgccgtcgg cctcgaccgc ggccttgatg gacagggccg ccgcgcaggc
     4861 ccgctccgcc gagtcctccc cctcgaagac ggcgacgacc tcgtcgccca cgaacttgtc
     4921 gatgtcgccg cccgcctcgt ggatcaggcg ggcctggacc tcgaggaggc ggttcaggac
     4981 ggcgacgacc tcctcgggcc cctttcgctc ggtgtaggag gtgaagcccc ggacgtcggt
     5041 gaagaggagg gtgcgggcga cccggctcgg ctcctggccc gccgccaggg cgtcgatcgt
     5101 gcccgaggag acgtacttcg tgagctcaaa cctctcgtag agccccctgg ccatctcgtt
     5161 caccgtgcgg gcgagctcgc ctatctcgtc cttgctccgg acctcgacgc ggccctcgaa
     5221 gcggcccttc gtgacgtcca ggcagagccg gccgatggag acgagcggcg ccaggacgac
     5281 ccgccggagg taggcgccca ggaccagggc gaggagggcg acgacggcca ggaagccgcc
     5341 cccggaggcg gcgatggtcc tctcctgggc ccgcgccacg gggctcacgt cgaccctgat
     5401 gtcgatgacg ccgcgcaacg tgtggtcggc gccgtggcag cccgtgcact tggggtagtt
     5461 gacgaggggc ctgaggctcc ggtagtagct ccggccgccc tcgctctccc ggaagaagag
     5521 ctccttgggc ggcttcgagg cggactccgc gaagcggggc ggcggctcgg ccggcgcctc
     5581 ggggccgggc cgcgggagga aacgctccct tccgatgttc gagttgaccc tcgctatggt
     5641 cgtcccgtcg gagaaggccc gctgccccgt cctcctgtac agcgcgaccg cggtgtcggg
     5701 gctcacggag gccacctcgg cgaagaagcg gaccgccacc ggcgcctcgc ccggcagcat
     5761 gaaattctcc acggaggtgg ccaggatcgc ggcctgctgg cccagggccg ccttggtgag
     5821 cgagtcgcgg gaggagacga gggccagggc gaaggcgaag accacggctc cgaccccggc
     5881 cgtgagggag acgaggatga tggcgttgac cttcgccgtg agggagaggc ccctggagtc
     5941 gccgcgtcgt gccatgcggt taatgatagt agacttcgcg cggttcgcaa gcgccccggc
     6001 gcgccggccc tcagtcctcc ccgcctccgg gggcgcggct cccggggccg tccgagtagt
     6061 agcgcttgat gaagcgttcc tgcgactcgt aggaccagtg gacctcccgc acgaaggcgg
     6121 ccgcccccgc gaagtcgccc cggtccaggc gcccgatgag ctcgccgtgc tcgcgcacgg
     6181 aggcgacctc ccactccttc acgaagccgg tgcgccgggg aaagtcgtag agcctctcct
     6241 tgaggatgtt gacggagcgc acgagctcgg cgttgtcgga gaggccgagg tagacgcgat
     6301 ggaactcgag gttggcggcg tagtaggcgg agaagtcgtc gcgggccagg gcctcgcccg
     6361 cctccgcgtt cagggcggcc atgcgcgccg agtcctccgg cccgtacttc cccttgaggc
     6421 cggcgatgac cgccgactcc agggcgccga tgagctcgta gacgttcttg atcgtctgca
     6481 ggtccaggga gcggaccatg acgccgcgcc gcgggtagat ccgcacgaag ccctcgatct
     6541 cgagccggat gagggcgtcc cggagcggcg tcctcgagag gccgagctcg cgtccgatgg
     6601 cggtgaggtc caggaaggcg ccgggcttga ggcgcccggc gttgaggaga tccttgagct
     6661 tcccgtagac cctctccctg aggagtcccg ggtcgaccga ttcgtcgtcg atgggcatgg
     6721 aagccactat aggcctgacc cgccgggccg gcaagggaaa aaggaggcgg ccgcccccgg
     6781 agggacggcc gcccgttttc gccgcgcggc gcgggccatt gcccggcccg cgcggcaggt
     6841 cgcccgcgag acgggctgcc ggtcctactt gcccgacatc atcgcggcga cgtcggcctt
     6901 cgggtcgacg atgcccttga tgccgaagct ctcgaccagg accttcgcaa ctccggggga
     6961 gaggaaggcg ggaagggtcg gcccgaggcg gatgttcttg acgcccaggg aaaggagggc
     7021 caggaggacg atgacggcct tctgctcgta ccaggcgatg tcgaaggaga ggggcag
//
LOCUS       DAOOSJ010000141         7028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6646_length_7028_cov_6.085903, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000141 DAOOSJ010000000
VERSION     DAOOSJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_7028_cov_6.085903"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLG14_07900"
     CDS             <1..952
                     /locus_tag="PLG14_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX49093.1"
                     /translation="ESAERARAELASSIESFLRVLHARRSEVRASFNYLLLASGVGIA
                     AVAVYAVWLWSRLRESGLRDEWSRRSLRHALEAEDAARRRIARDLHDDAAQDIAAAKM
                     LCDRAVGRAEAGAPGGDASGAGAGARAEAASGAEAARLAGEASALLRGAGLKLRSLAM
                     ELRPPELEGGGLVDALASLCASGRRGEAIGLHVGEDLPGIAPEAALHVYRVAQEGLAN
                     AAKHAKGNRIELRLERAFEGGRAWLVLEVLDSAEPTGAVRAPGPSAAADDPSLSSGTG
                     LSIMRERAALAGGRLDFERRADGAILRLSVPGLAPGKEGT"
     gene            949..1605
                     /locus_tag="PLG14_07905"
     CDS             949..1605
                     /locus_tag="PLG14_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX49094.1"
                     /translation="MKPRLLVVDDHPIFRSGVRNALADSGDYESVAEAGSVREAREAL
                     AGGGFGLVVADLGLPDGSGFDLVESFASEPGSPRFFVLSMNADRHSARKALRLGASGY
                     ASKNIGLVTLILGLRLVALGEVFIEGELLRDLLTAEAPEPRDRSLLRARFEALSPRER
                     DALDSLLEGLTTKEAASRLGVSHRTAENYQSSIYAKLGVESPLALLRLAARAGLVSLL
                     "
     gene            complement(1602..2267)
                     /locus_tag="PLG14_07910"
     CDS             complement(1602..2267)
                     /locus_tag="PLG14_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49095.1"
                     /translation="MSSPIVKAASFLRSRPRLAWPLCATALPLANALLCRLFKDLPLF
                     MDSIFTALAAAVLGPWQGLLVAILTNGYIEALTGFGGLHLPFALCGAATALLVAAAVR
                     SRRSWTPLGAAAAVLAVTLANAVLGAAIATFVYGGSTRVNIDSIAAGFAVVADSIFTA
                     AFLARLPINVIDKALAVLPALALAAALREAGGEGAPRAAVPCAARPGAAGQDAAGSGE
                     AAD"
     gene            2510..3514
                     /locus_tag="PLG14_07915"
     CDS             2510..3514
                     /locus_tag="PLG14_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX49096.1"
                     /translation="MKTKAIAFFLLVLCAAAAFAAPLPGRVVLGGKAVVMVADAVYLF
                     PSAKDRVVAVGGTDQGLGVFLGLVDPGFAAKPSLDRAAGAESYVALKPDLAVFKSAMK
                     KSLGPALDAAGVRQLYLDLETPEDYLRELKVLGEALGEGARGEALAAYYRAGMDRVAS
                     TVSAIPRGERPRVLVLQASGGGWEVPPASWMQSILAERAGGEPVWRDANPGSGWAKVG
                     PEQIAAWNPDAIFVINYREKAEASAAALRADPALASLRAAKAGRIHAFPQDFYSWDQP
                     DSRWILGLSWMAKKLHPSRFADLSVAAEAERFYAFAYGLDKARYEGAVKPRLSGGLDD
                     "
     gene            3570..4505
                     /locus_tag="PLG14_07920"
                     /pseudo
     CDS             3570..4505
                     /locus_tag="PLG14_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            4502..5257
                     /locus_tag="PLG14_07925"
     CDS             4502..5257
                     /locus_tag="PLG14_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49097.1"
                     /translation="MSLELRALSFRWPEGALALEGATLNLEPGRALALLGPNGAGKST
                     LLGLASGRLKPCAGEALLEGRPIAAWDRRALARRLAYLPQAERLPFNYYVLDFVLLGR
                     APHVPALSQPGPGDVERARAALAELGLSALEARPVTELSGGELQLARLARCLAQEADY
                     LLLDEPSTMLDPANARRLAEALRRLVAGGRALLFTSHDAAFSAYAAGEAALLKAGSLV
                     ALGPSRAVLVPDLLETAFGLPFKPTSLPSAFAE"
     assembly_gap    5262..5361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5380..6705
                     /gene="dgt"
                     /locus_tag="PLG14_07930"
     CDS             5380..6705
                     /gene="dgt"
                     /locus_tag="PLG14_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOX49098.1"
                     /translation="MEWKNLLEPRTAEGRATIGRTDFERDQDRVIFSSPFRRLDDKTQ
                     VLPIPERYFVHNRMTHSIETACVGRSLGSIVGARVLAAADPDWSDATTHELGDLVQAA
                     CLAHDIGNPPFGHSGEDAISAFFEGRPDLLEGLSEAERRDFTRFEGNAQGFRLISGAA
                     QGLRLTKNTAAAFAKYPRESLVSRYLDPRDSGRRDQKKYGAFQGERASLEDCLSAFGA
                     RRLSAEGLAFARYPFAHLVEAADDICYLVIDLEDAVRLGIIRLAEVEAELEAVVRDDP
                     ESGGLHSPAPGPRSGEAERVSYFRARAINSLIFQCAAVFAGRKAEILDGRYATSLTAE
                     IPSAGALEAIGRITREKLYRYRPVLEIEAAGFEIMGGLLELLTGAAFGLSGPRGRMIR
                     ELFPEIGLSEASPRYDRLRSVTDYVSGMTDSFAISEYKRLRGFELPRMY"
     gene            complement(6862..>7028)
                     /locus_tag="PLG14_07935"
     CDS             complement(6862..>7028)
                     /locus_tag="PLG14_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOX49099.1"
                     /translation="EVAASVFDRYRDDTYREASYKPYIIAAMILLGKAKDPAATLSRL
                     LERGTTREAL"
BASE COUNT          866 a         2681 c         2384 g          997 t
ORIGIN      
        1 cgagtccgcg gagcgggcgc gcgccgaact ggcctcctcg atcgagagct tcctccgcgt
       61 cctccacgcg cggaggagcg aggtccgcgc ctccttcaac tacctcctcc tcgcgagcgg
      121 cgtcggcatc gccgccgtcg cggtctacgc cgtctggctc tggtcgaggc tcagggagag
      181 cggcctccgc gacgagtggt ccaggaggag cctgaggcac gccctcgagg cagaggacgc
      241 tgcgcggcgc cgcatcgccc gcgacctcca cgacgacgcg gcccaggaca tagccgcggc
      301 caagatgctc tgcgaccgag cggtgggccg cgcggaggcg ggcgcgccgg gcggcgacgc
      361 atccggcgct ggcgcgggtg cgcgagcgga ggcggcttcc ggcgctgagg ccgcgaggct
      421 cgccggggag gcctcggccc tcctccgtgg cgccggtctc aagctccgct ccctcgcgat
      481 ggagctcagg ccgcccgagc tcgagggcgg gggcctcgtc gacgccctcg cgtccctctg
      541 cgcgagcgga aggcggggcg aggcgatcgg cctccatgtc ggggaggact tgcccggcat
      601 cgcccccgag gcggccctgc acgtctaccg cgtcgcgcag gagggcctcg ccaacgccgc
      661 caagcacgcg aaggggaacc ggatcgagct gaggctcgag cgggctttcg agggcggcag
      721 ggcctggctc gtcctcgagg tgctggacag cgccgagccg acgggcgccg tccgcgcccc
      781 cggcccctcc gcggcggcgg acgatccctc gctctcgagc ggtacgggac tctccatcat
      841 gcgcgagagg gccgccctcg cgggcggccg gctggacttc gagcggcggg ccgacggcgc
      901 gatcctcagg ctcagcgtgc ccggcctcgc gccgggaaag gaagggacgt gaaaccccga
      961 ctcctcgtcg ttgacgacca ccccatcttc aggagcggcg tgaggaacgc cctggcggat
     1021 tcgggagact acgagtccgt cgccgaggcc ggctccgtcc gcgaggcccg cgaagccctc
     1081 gcggggggcg gcttcggcct cgtcgtagcc gacctcggcc tccccgacgg gagcggcttc
     1141 gacctcgtcg agtccttcgc ctcggagccg ggctcgcccc gcttcttcgt cctctcgatg
     1201 aacgcggacc ggcactccgc caggaaggcc ctgcgcctgg gcgcctcggg ctacgcctcg
     1261 aagaacatcg gcctagtcac cctcatcctc ggcctccgcc tcgtggccct gggggaggtc
     1321 ttcatcgagg gcgagctcct gcgggacctc ctcacggcgg aagcgcctga gccgcgcgac
     1381 cgttccctgc tccgggcccg cttcgaggcc ctgagtcccc gcgagcgcga cgccctcgac
     1441 tccctcctcg agggcctcac gacgaaggag gccgcctcgc ggctcggcgt cagtcaccgc
     1501 accgcggaga actaccagag ctcgatctac gcgaagctcg gggtggaaag cccgctcgcc
     1561 ctcctcaggc tcgccgcgag ggccggcctg gtctccctgc tctagtccgc cgcctctccg
     1621 gaaccagccg cgtcctgtcc agccgcgccg ggacgcgccg cgcacggcac ggcggcgcgc
     1681 ggggctccct cccctcccgc ctcgcgcagg gcggcggcca gggcgagggc aggcaggacc
     1741 gccagcgcct tgtcgatgac gttgatcggc agccgggcca ggaaggccgc ggtgaagatg
     1801 gagtcggcga cgacggcgaa gcccgccgcg atcgagtcga tgttgaccct ggtcgagccg
     1861 ccgtagacga aggtcgcgat ggcggcgccg aggacggcgt tggccaaagt gacggcgagg
     1921 acggcggccg ccgccccgag gggcgtccag gagcggcggg aacggacggc ggccgcgacg
     1981 aggagggccg tcgcggcgcc gcagagggcg aagggcaggt ggaggccgcc gaagccggtc
     2041 agggcttcga tgtagccgtt ggtgaggatg gcgacgagga gtccctgcca ggggccgagg
     2101 acggcggcgg ccagggccgt gaagatcgag tccatgaaga ggggcaggtc cttgaagagc
     2161 cggcagagga gggcgttcgc caggggaagg gcggtcgcgc aaagcggcca ggccaggcgg
     2221 ggccggctcc tcaagaagga cgcggccttg acgatcgggg agctcatggt ttcagtgtag
     2281 gaccgccagc cgcctcgccg caagcggctt tcctggggct ccgtgaattt gcgaaagcga
     2341 tgtcgtgaga atacgccatt gtcccgcgaa aagcgtggct tcccggggac ttgatcctgc
     2401 cgtatagtgc ggtcagtaaa aatcatctaa aaagcactat ttactccgcc tgcctgtcgc
     2461 cgctttcctc gttgaccctg gcttcttccg gccgtattat cactccgcca tgaaaaccaa
     2521 agccatagct ttcttcctct tggtcctctg cgccgcggcc gccttcgccg ctccccttcc
     2581 cggccgcgtc gtcctgggcg gcaaggccgt cgtgatggtc gccgacgcgg tctacctctt
     2641 cccctccgcg aaggaccgcg tcgtcgcggt cggcggcacc gaccagggcc tcggcgtctt
     2701 cctcggcctc gtcgatcccg gcttcgccgc caagcccagc ctggacaggg cggccggggc
     2761 cgagtcctac gtcgcgctga agcccgacct cgccgtcttc aagagcgcga tgaagaagag
     2821 cctcggcccc gccctggacg cggccggcgt gaggcagctc tacctcgacc tggagacccc
     2881 ggaggactat ctccgcgagc tcaaggtcct gggcgaggct ttgggcgagg gcgcccgcgg
     2941 cgaggccctc gccgcctact accgcgccgg gatggaccgc gtggcctcca cggtctccgc
     3001 gatcccccgc ggcgagcggc cccgcgtcct cgtcctccag gcctccggag gcggctggga
     3061 ggttccgccg gcctcctgga tgcagtccat cctcgccgag cgcgcgggcg gcgagccggt
     3121 ctggcgcgac gcgaatccgg gctcgggctg ggccaaggtc ggccccgagc agatcgccgc
     3181 ctggaatccc gacgccatct tcgtgatcaa ctaccgcgag aaggcggagg cctcggcggc
     3241 cgccctcagg gcagaccccg ccctcgcctc gctccgcgcc gccaaggcgg gcaggatcca
     3301 cgccttcccc caggacttct actcctggga ccagccggac agccgctgga tcctgggcct
     3361 ctcctggatg gcgaagaagc tccatccctc ccgcttcgcc gacctttcgg tcgcggcgga
     3421 ggccgagcgc ttctacgcct tcgcctacgg cctcgacaag gcccgctacg agggcgccgt
     3481 gaagccgcgg ctgtcgggcg gcctcgacga ctaggccagc cccgagggcc gcgcgattcc
     3541 tttcaggctc cgcggccctc ctcgccctcc tctgcctcta ccttctcgcg gggcgctacc
     3601 cgaggccggg cctcaccgat cctcggctct tcctctccga cgagacggcg aggcgcctcc
     3661 tcctgggcgt gcgccttccc cgcgccctgg gcgccctcct cctcggggcc gtcctcggag
     3721 gcgcgggctc tgccttccag acggtcttcg gcaaccccct cgtcgagccc ggcttcctcg
     3781 gcgtctccca gggcgccgcg ctgggcgccg ccctggccct cgtcctgggc gcgggcgacc
     3841 cgaacctcgt cgccgtctcg gccttcgccc tggccctcct ctccctccgc gcgaccctcc
     3901 tcctcgcgaa ggccttccgc ttcggcggcc aggtcctccg cctcgtcctc tccggcatcg
     3961 cggtctccgc cttcgcctcc gccctcctgg ccttcgtgaa gtacgcggcg gaccccctct
     4021 ccgagctgcc cgacatcacc ttctggacga tgggcggcct ctcctcgatg ggctggggcc
     4081 gcctcgcggc cgcggccccc gtggccctcg cctcgctggc ggccctcttc ctcctgcgct
     4141 ggcgcgcgac catcctctcc ctggacgacg aggcctcccg ggccctgggc gctcggccgg
     4201 agctggagcg cggcgcggtc atcgtcgccg cgacggcggg ggtcgccgcg atgacggcgg
     4261 tttcgggcat cgtgtcctgg gtgggcctgg tcgtgcccca ggcggcgcgc gccctcgcgg
     4321 ggccggacgg ccgctcctcc atccccgcct ccatggccct gggcgccggc ttcgtcctcc
     4381 tctgcgacgg cctttcccgg agcctccttc cgggagagct ccccctgggc gtgccgacct
     4441 ccctcctggg cgcgctcgcc ttcgccctca tcctcgccgg caggcgggcg gcggtggcca
     4501 ggtgagcctc gagctcaggg ccctctcctt caggtggccc gagggagccc tggccctgga
     4561 aggcgcgacc ctcaacctgg agccggggcg ggccctggcc ctcctcgggc cgaacggcgc
     4621 ggggaagtcc acccttctcg gcctcgcctc gggacggctc aagccttgcg cgggggaggc
     4681 cctcctcgaa ggccggccga tcgccgcctg ggacaggcgg gccctcgcgc ggcgcctcgc
     4741 ctacctgccg caggccgagc gcctcccctt caactattac gtcctcgact tcgtcctcct
     4801 cggccgggcg ccgcacgtgc ctgccctctc ccagcccggc cccggcgacg tggagcgggc
     4861 ccgcgcggcc ctcgctgagc tcggcctttc ggccctcgag gcgcggccgg tgaccgagct
     4921 ctcgggaggc gagctccagc tcgcgcgcct ggcccgctgc ctggcccagg aggcggacta
     4981 cctcctcctg gacgagccct cgacgatgct cgatcccgcg aacgcccgcc gcctcgcgga
     5041 ggccctgcgc cgcctggtcg caggcggccg cgccctcctc ttcacgagcc acgacgcggc
     5101 cttctccgcc tacgccgcgg gagaggcggc cctcctcaag gcggggagcc tcgtcgccct
     5161 gggcccctcc cgcgccgtcc tcgtccccga cctcctcgag acggccttcg gccttccctt
     5221 caagcccacg agcctgccct ccgccttcgc cgaatagcgc cnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nggagagtat actcgcccca tggaatggaa gaatctcctc
     5401 gagccccgca cggccgaggg ccgcgcgacg atcggcagga cggacttcga gcgcgaccag
     5461 gaccgggtga tcttctcctc gcccttccgc aggctcgacg acaagaccca ggtccttccc
     5521 ataccggagc gctacttcgt ccacaaccgg atgacgcaca gcatcgagac ggcctgcgtc
     5581 ggccgctccc tggggagcat cgtcggcgcc cgagtcctcg ccgccgccga ccccgactgg
     5641 agcgacgcga cgacgcacga gctcggcgac ctcgtccagg cggcctgcct cgcccacgac
     5701 atcggcaacc cgcccttcgg ccattcgggc gaagacgcga tctccgcctt cttcgagggg
     5761 cgtcccgacc tcctcgaggg gctttcggag gcggagcgcc gcgacttcac gcgcttcgag
     5821 ggcaacgccc agggcttcag gctcatctcc ggcgccgccc agggcctcag gctcaccaag
     5881 aacacggcgg ccgccttcgc gaagtacccc agggagagcc tcgtgtcccg ctacctcgat
     5941 ccccgggact cgggcaggcg cgaccagaag aagtacggcg ccttccaggg cgagcgggcc
     6001 agcctggagg actgcctctc cgccttcggc gcgcgccgcc tctcggcgga gggcctggcc
     6061 ttcgcccgct atcccttcgc ccacctcgtg gaggccgcgg acgacatctg ctacctcgtc
     6121 atcgacctgg aggacgcggt gcgcctcggg atcatccgcc tcgcggaggt ggaggccgag
     6181 ctcgaggccg tcgtccgcga cgaccccgag tcgggcggcc tccactcccc ggccccgggg
     6241 ccgcggagcg gcgaggccga gcgggtctcc tacttccgcg cgcgggcgat caacagcttg
     6301 atcttccagt gcgccgccgt gttcgccgga aggaaggcgg agatcctgga cggccgctac
     6361 gccaccagcc tcaccgccga gataccgagc gccggggccc tggaggccat cgggcgcata
     6421 acgcgcgaga agctctaccg ctaccggccc gtcctggaga tcgaggcggc cggcttcgag
     6481 atcatgggcg gcctcctcga gctcctcacg ggggcggcct tcgggctctc ggggccgcgc
     6541 ggccgcatga tccgcgagct cttccccgag ataggcctct ccgaggccag cccccgctac
     6601 gaccgcctcc ggagcgtcac ggactacgtg tccgggatga cggactcctt cgcgatctcc
     6661 gaatacaagc ggctgcgggg tttcgagctc ccgcggatgt actaggagcc cgccggggcg
     6721 cggccgccct ccggccccgc gcccgccgcc tcgggaccgg gccagggcaa gccccgctcg
     6781 cgaaaaaaag gaaagggccg ccccgggaca tgcccggggc ggcccttcgg cgtccggcgc
     6841 ggccgcggcc gcgccttcgg cctagagggc ttccctcgtc gtcccgcgct cgaggaggcg
     6901 ggagagggtc gcggcgggat ccttggcctt gccgaggagg atcatcgcgg cgatgatgta
     6961 gggcttgtag ctcgcctcgc ggtaggtgtc gtcgcggtag cggtcgaaga cgctcgcggc
     7021 gacctcgc
//
LOCUS       DAOOSJ010000142         6974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6714_length_6974_cov_5.838127, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000142 DAOOSJ010000000
VERSION     DAOOSJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6714_length_6974_cov_5.838127"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1032
                     /locus_tag="PLG14_07940"
     CDS             103..1032
                     /locus_tag="PLG14_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49100.1"
                     /translation="MESSRHLRLGVPGEGRPAPSLIYTFMARRSGGLSLGINLFPDGK
                     DCSFDCPYCEVFPQPGGAAFDPALLREELAEFASVYAAGLPPLRDISLAGKGEPTLSP
                     FLPEALAAAAEARRLFPAALGSASLVVITNSTGFLEAGTADLLRRYVEEEGLVVWAKL
                     DAGTEAWYRRVNRCPIPFEKLLSGLEAFARESPVVLQTMLCALPPEGVPAGTADPAGT
                     AGRGGATGLPEAEGPSEGELGAYAERVLSLLGAGARIRELHLYTQSRPSFEGLSAALP
                     DAELLRLAALLRPRLPLPIRVFGSEGELEAAAR"
     gene            1029..1985
                     /locus_tag="PLG14_07945"
     CDS             1029..1985
                     /locus_tag="PLG14_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOX49101.1"
                     /translation="MIDLHSHSTASDGSLAPAALVALARERGLRALALTDHDTMAGLP
                     EAEEAAALPGPGAGGGAAPASSAAAAGPAGQSGPAGSGSPEAAPFRLIRGVEIEIAFS
                     PGEFHLLGLDLAGPDAPDPFAEGLARLAEALEGLGRARDDRNRKIIERINAAGIQATM
                     EELGALAGGSRPGRPHIASLLVARKAAKSRQDAFSRFLAKGRLFYEPKETLGLPEAVA
                     LVKAAGGLAVVAHPYSLFVSKARLALLMDEWKELGVDGIEAYHPTAKLGQCRRLEKMA
                     RERNFRVTAGSDYHGAIRPERRLGRTAGGLVIEDSYLDELSR"
     gene            complement(2152..3282)
                     /locus_tag="PLG14_07950"
     CDS             complement(2152..3282)
                     /locus_tag="PLG14_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOX49102.1"
                     /translation="MEGKNVRRALFLVFFLALFLLVARLLYPFLTNLLWSGLIYALFA
                     PLYNRFEKRKDGRLRGVFARNAFASFLSLFGVLVFVVPLAFLGMSMARQLGDLTASAL
                     RLLENHPEFLDLSPQGTVGGFIYRLTEGSVDLSSIDLRGELYGLFAGSSNRIIGFSGA
                     VLKNAASFLLTLAFMVFTLYFFFVDGGHLIRILVNAIPIERSYTRLFLRKFRDTSRQL
                     VVGFFLVALYQAVVAFILFTLFGVRGALVLAALTGVASFIPMVGAGLVWAPVAAIRMV
                     EGNMAGGIALFVLSALLISTLDNFIRPLLLGERLQLHPLLIFFSILGGLSLFGFNGLV
                     LGPLILILFFTGVRLFDQTYDREADDGGEAELGEAEREGPGE"
     gene            complement(3285..3734)
                     /locus_tag="PLG14_07955"
     CDS             complement(3285..3734)
                     /locus_tag="PLG14_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49103.1"
                     /translation="MALALVLAFSALGLAAALLAYLAGRLMPEGRKDGKGKGRGGLLR
                     LGQESGRAGQAGPAARSCPLCGSTLGPGERVKSDLSPGKGDRIMRIFGCPRCWPALPG
                     AKARTCPVCRGEIPPGGHAVARYFESPGRKHVHVLGCSACRERGKVV"
     gene            3876..>4141
                     /locus_tag="PLG14_07960"
     CDS             3876..>4141
                     /locus_tag="PLG14_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX49104.1"
                     /translation="MATLSVVATPIGNLGDITLRALETLKSVDVIACEDTRHTLKLLS
                     HYGIRKSLVACYAYDEEKGAARILGLLAEGKNVAYCSDAGTPGLS"
     assembly_gap    4142..4151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4152..4616
                     /locus_tag="PLG14_07965"
     CDS             <4152..4616
                     /locus_tag="PLG14_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX49105.1"
                     /translation="EFWKRELAGARGTPGLSDPGALAVRKAREAGYKVEPIPGPSAFA
                     ALLSAAGLGGRAVLFEGFLSPKPGRRRSRVVELMAREEAFLVYESPHRVAKLLAEVVA
                     VDPERRVCVGREMTKIHEEFVVGPASEALSRFSGDEEPKGEFAVLVAGAKLA"
     gene            4667..4951
                     /locus_tag="PLG14_07970"
     CDS             4667..4951
                     /locus_tag="PLG14_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOX49106.1"
                     /translation="MTIDRLNSIDPIRDPQKSSQAGRTSRAAKGDSIAISNDAAEKAE
                     LYNAVEIAKAAPEVRTDRVAELKAKIQDPGYLNDAVISMTADKILDQLLG"
     gene            5061..6221
                     /locus_tag="PLG14_07975"
     CDS             5061..6221
                     /locus_tag="PLG14_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX49107.1"
                     /translation="MADFTFSVRPRISLGPDTLLRLPLFAGEYAGSASPRALLVVDPL
                     LSESKTIDRVRSLLEDRGVQVLLFDELPDRATSRAADDALRLARGARAPLVIGLGGLK
                     TLMVARAVAAVAPSRLDIDAVLDGQSTGCPSLPLIEIPTSLRHGFMTDESFFLVDGRD
                     RRVRLVRSPGSGPVAVLVDPNLSASLSPKLASTCVLAGLMGAVESYVSARSSFMSDLL
                     LERAVSSLAKALDILVARPDDPTARVEAWRGAFLASVGQGLSAPGIGTALSLAINARY
                     PVPKSSLAAILLPYSMESASKSRLEKVAALLPLLGEEADGMPDGEAAAKSVEWLRTRL
                     GVLKIPSRLKDFDLSLDRLVETARDARELDFVNYLPRAVSVEDVFDFVKTAF"
     gene            6224..>6974
                     /locus_tag="PLG14_07980"
     CDS             6224..>6974
                     /locus_tag="PLG14_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOX49108.1"
                     /translation="MIPLAKLARLHPRHRLRKAALVLSEVERRLRAPYPLAGQEADRA
                     AAESYAAALCAYLAADADCPGELRARAGRAAEAAAALAAALAAAPAAAAPARSIDAAA
                     SALARSIDAAASAPARAGDGRAVRAWEGGGERARAALVRAVDELRHALLRETGQAPAD
                     WDLLDPATGRPDAAARRVRPGMRAYLEDLRSPFNVGSMFRTADAFGLEELLLSPDTAD
                     PAHPRSARSSMGATALVPWRRAGLEALGGPSA"
BASE COUNT          970 a         2642 c         2368 g          984 t
ORIGIN      
        1 cgcctggagc ggcgcggccc ggcgcctggc cgctcttcgc ggcgggcacg gcgcgcccgc
       61 tccgcttgca ggaggcgagg gcgaagggct agtatccctg ccatggaatc ctcccgccac
      121 ctccgcctgg gcgttccggg cgagggccgc ccggccccga gcctcatcta taccttcatg
      181 gccaggcgct cgggcggcct ttcgctcggc atcaacctct ttcccgacgg gaaggactgc
      241 tccttcgact gcccctactg cgaggtcttc ccccagccgg gaggcgcggc cttcgatccc
      301 gccctcctcc gcgaggagct cgccgaattc gcctccgtct acgccgccgg cctaccgccc
      361 ctccgcgaca tctccctcgc ggggaagggc gagccgacgc tgtctccctt cctcccggag
      421 gccctggccg ccgccgccga ggcgcgccgc ctctttcccg cggccctcgg ctcggcctcc
      481 ctcgtggtca tcacgaactc gaccggcttc ctggaggcgg gcacggcaga cctccttcgc
      541 cgctacgtcg aggaggaggg cctcgtcgtc tgggccaagc tcgacgcggg cacggaggcc
      601 tggtaccgcc gcgtgaaccg ctgccccatc cccttcgaaa agctcctctc cggcctcgag
      661 gccttcgcgc gggagagccc cgtcgtcctc cagaccatgc tctgcgccct ccctcccgag
      721 ggcgttccgg ccgggacggc cgacccggcc ggaacggcgg ggaggggcgg ggccacgggc
      781 ctcccggagg cggagggccc ctcggagggc gagctcgggg cctacgccga gcgcgtcctc
      841 tcgctccttg gcgccggggc caggatccgc gagctccacc tctatacgca gtccaggcct
      901 tccttcgagg gcctctcggc cgcgttgccc gacgccgagc tcctccgcct cgcggccctg
      961 ctccggccgc ggctcccgct gcccatccgg gtcttcggct ccgagggaga gctcgaggcg
     1021 gcggcgcgat gatcgacctg cacagccact ccaccgcgag cgacgggagc ctcgcgccgg
     1081 cggccctcgt ggccctggcc cgggagcgcg gcctccgcgc cctggccctc acggaccacg
     1141 acacgatggc cggcctgccc gaggccgagg aggcggccgc cctgcccggg cccggcgctg
     1201 gcggaggggc cgcgccggcc tcgtccgcag cggccgccgg ccccgcgggg caatccgggc
     1261 ccgccgggag cggctccccg gaggccgctc ccttccgcct catccgcggc gttgagatcg
     1321 agatcgcctt ctctcccggg gagttccacc tcctcggcct cgacctcgcg ggtccggatg
     1381 cccccgaccc cttcgcggag ggactcgcgc gcctcgccga ggccctggag ggactcggcc
     1441 gggcgaggga cgacaggaac cgcaagatca tcgagaggat caacgcggcg gggattcagg
     1501 cgacgatgga ggagctcggt gcgctggccg ggggctcgag gccgggtcgg ccgcacatcg
     1561 cgagcctcct cgtggcccgg aaggccgcga agagcaggca ggacgccttc tcccgcttcc
     1621 tcgccaaggg ccggctcttc tacgagccca aggagaccct cggcctgccc gaggcggtgg
     1681 ccctcgtcaa ggcggcgggc ggcctggccg tcgtcgccca cccctattcc ctcttcgtct
     1741 ccaaggcgag gctcgccctc ctcatggacg aatggaagga gctgggcgtg gacgggatcg
     1801 aggcctacca ccccacggcc aagctggggc agtgccgccg cctggagaag atggcccgcg
     1861 agaggaattt ccgcgtgacg gcggggagcg actaccacgg cgcgatccgg cccgagcgcc
     1921 gcctcggccg cacggcgggc ggcctcgtca tcgaggattc ctacctggac gagctctccc
     1981 gctaggcctc cgcggatccg cgccgcccgc tctcctgggc cgggcttgcg cctgccgctg
     2041 ggcccggcgc ggatccgcgc tgcccgctcc cgtagtccgg gccggcgccc gccgcggagc
     2101 ctggcgccgg ccgcggagcc cctcggcagg ctccgccccg ccaggcggcc cttattcccc
     2161 gggcccttcc cgttccgcct ctcccagctc cgcctccccg ccgtcgtcgg cctccctgtc
     2221 gtaggtctgg tcgaagagcc gcacgcccgt gaagaagagg atgaggatga gggggcccag
     2281 gaccaggccg ttgaagccga agaggctcaa gccgcccagg atggagaaga agatgaggag
     2341 gggatggagc tggagccgct cgcccaggag gagggggcgg atgaaattgt ccagggtgga
     2401 gatgaggagg gccgagagga cgaagagggc gatgccgccc gccatgttcc cttcgaccat
     2461 gcggatcgcc gcgaccgggg cccagacgag gccggcgccc accatgggga tgaaggacgc
     2521 gacgcccgtc agggcggcca ggacgagggc gccgcgcacg ccgaagaggg tgaagaggat
     2581 gaaggccacg acggcctggt agagggcgac gaggaagaag cccacgacga gctggcggct
     2641 cgtgtcccgg aatttcctca ggaagagccg cgtgtaggac cgctcgatgg ggatggcgtt
     2701 gacgaggatc ctgatgaggt ggccgccgtc cacgaagaag aagtagaggg tgaagaccat
     2761 gaaggcgagg gtgaggagga aggaggccgc gttcttgagg acggcgcccg agaagccgat
     2821 gatgcggttc gagctgccgg cgaagaggcc gtagagctcg ccgcgcaggt cgatggagga
     2881 gaggtcgacc gagccctcgg tcagcctgta gatgaagccg cccaccgtcc cttggggcga
     2941 gaggtcgagg aattccggat ggttctcgag gagccgcagg gcggaggcgg tgaggtcgcc
     3001 gagctgcctg gccatgctca tccccaggaa ggcgagggga accacgaaga cgaggacgcc
     3061 gaagagggag aggaagctcg cgaaggcgtt ccgcgcgaag acgccgcgca gcctgccgtc
     3121 cttgcgcttc tcgaagcggt tgtagagggg cgcgaagagg gcgtagatga ggcccgacca
     3181 gaggaggttc gtgaggaatg ggtagaggag cctggcgacg aggaggaaga gcgcgaggaa
     3241 gaatacgagg aacagggccc tcctgacgtt tttcccttcc atggctagac gacctttccc
     3301 cgctcgcggc aggcgctgca tcctagcacg tgcacgtgct tccgtcccgg gctttcgaag
     3361 tagcgggcca cggcgtgccc ccccgggggg atctcgccgc ggcagacggg gcaggtccgc
     3421 gccttggctc cgggcagggc gggccagcag cggggacagc cgaagatccg catgatcctg
     3481 tcgccctttc ccgggctcag gtcggatttc acccgctccc ccggtcccag ggtcgagccg
     3541 cagagcgggc agctccgcgc ggcgggcccg gcctggccgg ctcgcccgga ctcctggccg
     3601 agccggagga ggcctcctcg gcctttgccc ttcccgtcct tccggccttc gggcatgagc
     3661 ctgcccgcca ggtaggcgag gagggccgcg gcgaggccga gggccgagaa ggcgagaaca
     3721 agggcgagag ccacggcctg aattatagcc ttggccggcc cgagcccgct aggtccggcg
     3781 ttccggatcc gcgtggattc cgcgcgcggg atcccttgcg cgggatcccg ggcctcgcct
     3841 tgaccccccg cccggccccg gcccatactc cccccatggc aactctctcc gtcgtcgcga
     3901 cccccatcgg caacctgggc gacatcaccc tgcgcgccct agagaccctc aagtcggtcg
     3961 acgtgatcgc ctgcgaggac acccggcata ccctgaagct cctttcccac tacgggatca
     4021 ggaagagcct ggtcgcctgc tacgcctacg acgaggaaaa gggcgccgcc aggatcctcg
     4081 ggctcctggc cgagggcaag aacgtcgcct actgctcgga cgcgggcacg cccggcctct
     4141 cnnnnnnnnn ngagttctgg aagcgtgagc tcgcgggtgc ccgaggcacg ccgggcctct
     4201 ccgaccccgg cgccctggcc gtccgcaagg cccgcgaggc gggctacaag gtcgagccca
     4261 tccccggccc gagcgccttc gccgccctcc tctccgcggc cggcctgggc ggccgggccg
     4321 tcctcttcga gggtttcctc tcccccaagc cggggcgcag gcggagccgg gtcgtggagc
     4381 tcatggccag ggaggaggcc ttcctcgtct acgagtcccc ccaccgcgtg gccaagctcc
     4441 tggccgaggt cgtcgccgtc gatccggagc gccgggtctg cgtgggccgc gagatgacga
     4501 aaatccacga ggaattcgtc gtcggccccg cctccgaggc cctgtcccgc ttctccggcg
     4561 acgaggagcc caagggagag ttcgcggtgc ttgtcgccgg cgcaaaattg gcgtaaacta
     4621 tttcaaggcc atcgccgata attcaggtga aaggcagggg cgccatatga ccattgatcg
     4681 cctaaactcc atagacccga tccgggaccc ccagaagtcc tcccaggctg gaaggacttc
     4741 ccgcgccgcg aagggcgatt ccatcgccat ctccaacgac gcggcggaga aggccgagct
     4801 ctacaacgcc gtggagatcg ccaaggcggc ccccgaggtc aggaccgacc gcgtcgccga
     4861 actcaaggcg aagatccagg atcccggcta cctgaacgac gccgtcatct ccatgaccgc
     4921 ggacaagatc ctcgaccagc tcctcggcta aggccgtctt tccttcgaga gcccacgggc
     4981 gggagcgagt cttccgccct tttattttat caggaaggga aggggcttcc acctgatgga
     5041 agccctgcgg gaacgagcgc atggctgatt tcaccttctc ggtcaggccg aggatctctc
     5101 tcggccccga caccctcttg cggcttcccc tcttcgccgg cgagtacgcc ggctcggcct
     5161 ccccccgcgc cctcctcgtc gtcgaccccc tcctttccga gtccaagacc atcgaccgcg
     5221 tccgctccct cctggaggac aggggcgtcc aggtcctcct cttcgacgag ctccccgaca
     5281 gggcgacgag ccgcgcggcc gacgacgccc tccgcctggc ccgcggggcc cgcgcccccc
     5341 tcgtcatcgg cctgggcggc ctcaagaccc tcatggtcgc ccgcgccgtg gccgccgtcg
     5401 ccccctcccg cctcgacatc gacgcggtcc tcgacggcca gtcgacgggc tgcccctccc
     5461 ttcccctcat cgagataccg acctccctgc gccacggctt catgacggac gagtccttct
     5521 tcctcgtcga cggccgcgac cggcgggtcc gcctggtccg ctcgcccggc tcggggccgg
     5581 tcgccgtcct cgtcgacccg aacctctccg cgagcctatc ccccaagctc gcctccacct
     5641 gcgtcctcgc gggcctcatg ggcgccgtcg agtcctatgt ctcggcccgc tcctccttca
     5701 tgagcgacct cctcctggag cgcgccgtct cctccctcgc gaaggccctg gacatcctgg
     5761 tcgcgaggcc cgacgacccg acggccaggg tcgaggcctg gcgaggggcc ttcctcgcct
     5821 ccgtcggcca gggcctttcc gcccccggca tcggcacagc cctctccctc gccatcaacg
     5881 cccgctatcc cgtgcccaag tccagccttg ccgccatcct ccttccgtat tccatggaga
     5941 gcgcctccaa gagccgcctg gagaaggtgg ccgccctcct tcccctcctg ggcgaggagg
     6001 ccgacgggat gcccgacggc gaggcggcgg cgaagtccgt ggagtggctc aggaccaggc
     6061 tcggcgtcct caagatcccg agcaggctca aggacttcga cctctcgctc gaccgcctgg
     6121 tggagacggc ccgcgacgcg agggagctcg acttcgtgaa ctacctgccc cgggccgtct
     6181 ccgtcgagga cgtcttcgac ttcgtgaaga ccgctttcta gccttgatcc cactcgccaa
     6241 gctcgcccgc ctccaccccc ggcaccgcct caggaaggcg gccctcgtcc tctccgaggt
     6301 cgaacggcgg ctcagggctc cctacccgct cgctggccag gaggctgacc gggccgccgc
     6361 ggagtcctac gccgcggccc tctgcgccta cctcgccgcc gacgccgact gcccggggga
     6421 gctccgcgcc cgcgccgggc gcgccgccga ggccgccgcg gccctggcag ccgccttggc
     6481 cgccgcgccc gccgccgcgg cgccggcccg ctctattgat gcggccgctt cggcgctagc
     6541 ccgctctatt gatgcggccg cttcggcgcc ggcccgcgcc ggggatggcc gggcggtccg
     6601 cgcctgggag ggcggaggcg agagggccag ggccgccctt gtccgcgccg tcgacgagct
     6661 caggcacgcc ctcctaaggg agacgggcca ggcgccggcc gactgggacc tccttgaccc
     6721 cgcgaccggg cggccggacg ccgccgcccg ccgcgtgcgc cccgggatgc gcgcctatct
     6781 cgaggacctg cgctccccct tcaacgtagg ctccatgttc cgcacggcgg acgccttcgg
     6841 cctcgaggag ctgctcctct cgcccgacac ggccgacccg gcccatcccc gttcggcccg
     6901 aagctcgatg ggggcgacgg ccctcgtgcc ctggcgccgc gccggcctcg aggccctggg
     6961 cggcccctcg gcta
//
LOCUS       DAOOSJ010000143         6942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6746_length_6942_cov_5.560621, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000143 DAOOSJ010000000
VERSION     DAOOSJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6746_length_6942_cov_5.560621"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLG14_07985"
     CDS             complement(<1..817)
                     /locus_tag="PLG14_07985"
                     /inference="COORDINATES: protein motif:HMM:NF028196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOX49109.1"
                     /translation="MSASGGAEAGGRIVGRITRVSGPVVLAEGLGGAGLYDVVEAGEA
                     GLAGEIIRMDASGATIQVYEENTGMRPGEPVVSLGRPLSALLGPGILGSIYDGIQRPL
                     DGLKRMSGSFLAPGVKGERLDPGRKWDFEPALREGGPVGPGTLLGVVRETSVVEHRVL
                     APPGTARGRASYVAPAGGYTIRDCVARVEPEGGSARRGGAERAPAELFLAHEWPLRRP
                     RPASRRLPPDEPLVTGLRVIDVLFPLSKGGAAAVPGGFGTGKTMTQHAIAKWCD"
     gene            complement(814..1431)
                     /locus_tag="PLG14_07990"
     CDS             complement(814..1431)
                     /locus_tag="PLG14_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49110.1"
                     /translation="MEEIASTEALEREILEDSRRKSERVLKEADEESRAVQAQAEERA
                     AKAFASLEADYAARLDRLRAESLARVPLEKARLKAAHVHARVAGLVASRLAALSAERK
                     AALVRALALEAGAACRSRFASGAALGFRGLSPEEAARIAAEALPGVRVAAPAEDPGLP
                     AAGLLLEAADGSLSLRATVDLLGERLLDERRGELALSLCAKAMSL"
     gene            complement(1444..1866)
                     /locus_tag="PLG14_07995"
     CDS             complement(1444..1866)
                     /locus_tag="PLG14_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOX49111.1"
                     /translation="MPDFFFIGEEELCVAFRLVGVGGKAVANREEAVEAFLEATGRPS
                     GLLRPSAGAAPDGALLDGASRPEAASAGLSEEAPSRRAAKVLVLSSRTAALLEEETRD
                     WQFGSEYPLVVEIPSPGAGADERRSLVAAIREAVGISI"
     gene            complement(1884..2204)
                     /locus_tag="PLG14_08000"
     CDS             complement(1884..2204)
                     /locus_tag="PLG14_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOX49112.1"
                     /translation="MSKRASIFFVLFALAAFAAAAQEAAAAPAAAAAASAAGSSLKYF
                     AAALAVGIACIAGGIAVGQIGSAAMGAMAENPELSGKALPYAGLAEGICLWGFLVGLL
                     ILLL"
     gene            complement(2208..4205)
                     /locus_tag="PLG14_08005"
     CDS             complement(2208..4205)
                     /locus_tag="PLG14_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOX49113.1"
                     /translation="MSPVAMRKLELFILKNDMDRVLEILGRAGCFELAEQRRSEGEPR
                     PGRDPRVEAALDKLRKLRDLLDLEAPDLVPEGTRLPGPREDALLGELVAAAEGLSAEL
                     AALQARRGRIGEALEEARAFAGLSLPFKELDHLSFLAVRVGRVPPSELPALAANLGDR
                     AILLPLGEDGSVVAAASKKGRFALDTELERAKFRPRELPPDFKGLPPELVASLEAERA
                     ALDAEASRLEARKAALRASVGDSWESLAASYAVLASIERAKEGLESSDFVYRLEGWAP
                     KARAAALLKELGDATGGRAAARVYDPEELDSVRSGEEKVPVLLKRRPFVSSFEGLVVS
                     YGAPLYGTIDPTPLVAFFFVVLFAIMFGDLGQGAVILLAGLVIGRGKVAKLRKWKAYG
                     PIFTAVGLGSMAMGLLDGTVFANEEWLVPASRAVSLALLGRPLDRFVTVMPEGGSVAK
                     LFAFFGFTLGVGVLINSVGLVVNIVNKWRLGKKGEALFSKTGLAGSLFFWWAIGLGVR
                     VAAGGRPSWPDIPGFALPLAALFLEEPLGRLVEGRKREGKGDGALAFAIKGLVEVIES
                     VSYYFSNSLSFLRVGAFALSHAVLSFIVFAMGDLVASRAPGGVLWEVLVVVAGNAVII
                     VLEGMIVAIQVVRLQYYEFFSKFFTETGTLFAPFRFEYPKE"
     gene            complement(4202..5269)
                     /locus_tag="PLG14_08010"
     CDS             complement(4202..5269)
                     /locus_tag="PLG14_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOX49114.1"
                     /translation="MADRYELAYVYARVCGSLAGSLAGGRAAELLRIGRLADAWRAAF
                     GDAPPALPERSLVAAAESRAVREAQADFVALAASLRGEEPFFVALRRKAEFGRVKRVL
                     LAVREGALHCPESFDPEYPAGFDESAYPSLGSMFSGGRYGWLSDKALEDLPAAENRLD
                     RQFYEELWEALRSVPSRRLGSLRELVALEAELENVVWALRLARYYGMDLPRIESLLVA
                     LPGADVVSAALEGARLAGARDGGAGGGAEGRTARRAARRAAKPRGDAPLEDWKYAFLA
                     EGFQAGGIDPRTVEARARRYLYGRLRRALHRHPFTYTPLYCFFKLKEFEAAVVLGLVE
                     GIYLGAPAEEIAWLAPAGGVA"
     gene            complement(5275..5583)
                     /locus_tag="PLG14_08015"
     CDS             complement(5275..5583)
                     /locus_tag="PLG14_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49115.1"
                     /translation="MPSRELLDRLFAVESEARALLDEAREEARRRVAAAKEEAETAFK
                     RAYERKAAELERGFAEGRATCEAEYASLISSYREALASAPEDEAAFDAVFDAFLDLKA
                     "
     gene            complement(5619..6095)
                     /locus_tag="PLG14_08020"
     CDS             complement(5619..6095)
                     /locus_tag="PLG14_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOX49116.1"
                     /translation="MLLQQVFTPARIKVGLESGEKDELFEELVDLLARSGGRDFPRRN
                     VLLAVREREAKMSTGIKKGIALPHGKAEGIEGLVGAIGVSRSGVDYDSLDGEPVYLVF
                     MLISSTKDSELHLSALKRLARLLDDPEFYTELVAAETPERAFEILKSFEDLLTLQR"
     gene            complement(6141..6548)
                     /gene="secG"
                     /locus_tag="PLG14_08025"
     CDS             complement(6141..6548)
                     /gene="secG"
                     /locus_tag="PLG14_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOX49117.1"
                     /translation="MEFQTMGILGIVLLVVFVIVSILLVLLVIVQDEGSESLGGIFAG
                     GSSSAFGSRSSNVVIKVTYVLGGLFFVLAFSLAIVNKGSVGNVEAAAQKKGDAAASEW
                     WNAAPQGAAPAELAPASGDLQAPAAPAPAPAGK"
BASE COUNT         1048 a         2407 c         2625 g          862 t
ORIGIN      
        1 cgtcgcacca cttcgcgatg gcgtgctggg tcatggtctt gccggtgccg aagcccccgg
       61 gcaccgccgc ggcgccgccc ttggagagcg ggaaaaggac gtcgatgacg cggaggcccg
      121 tgacgagcgg ctcgtcgggc ggaaggcggc ggctcgccgg ccgcggccgc ctgagcggcc
      181 actcgtgggc caggaagagc tccgccgggg cgcgctcggc cccgccccgc cgcgcggagc
      241 cgccctccgg ctccacgcgg gccacgcagt cccggatcgt gtagccgccc gccggcgcga
      301 cataggaggc ccgcccccgc gccgtcccgg gcggcgccag gacgcggtgc tcgacgacgg
      361 aggtctcccg cacgacgccc aggagggtgc cgggcccgac gggcccgccc tcgcgcaagg
      421 cgggctcgaa atcccacttc cgcccgggat cgagccgctc gcccttcacg ccgggggcca
      481 ggaaggagcc ggacatgcgc ttgagcccgt ccaggggccg ctggatgccg tcgtagatgg
      541 agccgaggat gcccggcccg aggagggccg agagcggcct cccgagggag acgacgggct
      601 cgcccggcct catgccggtg ttctcctcgt agacctggat ggtcgcgccc gaggcgtcca
      661 tcctgatgat ctcgcccgcg agcccggcct cgcccgcctc gacgacgtcg tagaggccgg
      721 ccccgcccag gccctcggcc aggaccacgg gccccgagac ccgcgttatg cggccgacga
      781 tcctgccgcc ggcctccgcc cctcccgagg cgctcacagg gacatcgcct tggcgcagag
      841 ggagagcgcc agctctccgc ggcgctcgtc taggaggcgc tcgccaagga ggtcgacggt
      901 ggccctcaag gagaggctcc cgtcggccgc ctcgaggagg aggccggcag cgggaaggcc
      961 cgggtcctcc gccggcgcgg cgacgcggac gccgggaagc gcctccgcgg cgatcctggc
     1021 cgcctcctcg ggggagaggc ccctgaagcc cagggccgcg cccgaggcga agcggctccg
     1081 gcaggccgcc ccggcctcca gggccagggc gcggaccaag gcggccttcc tctccgcgga
     1141 aagggcggcc agccgggacg cgaccaggcc ggccacgcgg gcgtggacat gggcggcctt
     1201 gaggcgggcc ttctcgagcg ggacgcgcgc gaggctctcc gccctcagcc tgtccagccg
     1261 ggccgcgtag tcggcctcca gcgaggcgaa ggccttcgcg gcccgctcct ctgcctgggc
     1321 ctggacggcg cgcgactcct cgtccgcctc cttgaggacc cgctccgact tccgcctcga
     1381 gtcctcgagg atttcccgct cgagcgcctc ggtagatgca atctcttcca tacggctacg
     1441 ctcctagatg ctgatgccga ccgcttcccg tatcgcggcg acgaggctgc gcctctcgtc
     1501 cgcgcccgcg ccggggctcg ggatctccac gacgagggga tactcgctgc cgaactgcca
     1561 gtcgcgcgtc tcctcctcga ggagggcggc cgtccgcgag ctcaggacga ggaccttggc
     1621 cgcgcgccgg gacggggcct cctcggacaa gcccgcggag gcggcctccg gcctggaggc
     1681 gccgtcgagg agggcgccgt ccggcgcggc cccggcggac ggccgaagga ggccggaggg
     1741 ccgccccgtc gcctcgagga aggcctccac ggcctcctcg cggttggcga cggccttgcc
     1801 gccgacgccg accagcctga aggcgacgca gagctcttcc tccccgatga agaagaagtc
     1861 cggcatggcg gcgcgccccc gctctagagg aggaggatga ggaggccgac gaggaagccc
     1921 cagaggcaga tgccctccgc caggcccgcg tagggcaggg ccttgccgga gagctcgggg
     1981 ttctccgcca tggcgcccat cgccgcggag ccgatctgtc cgacggcgat gccgccggct
     2041 atgcaggcga tgccgacggc cagcgcggcc gcgaagtact tgagggagga cccggcggcc
     2101 gaggccgccg cggcggccgc gggagcggcc gcggcctcct gcgcggcggc cgcgaaggcg
     2161 gccagggcga acaggacgaa gaagatgctc gcgcgtttgc tcattgctca ctccttggga
     2221 tattcgaagc ggaagggggc gaagagcgtc cccgtctcgg tgaagaactt ggagaagaac
     2281 tcgtagtact ggagcctgac cacctggatc gcgacgatca tgccctccag gacgatgatg
     2341 accgcgttcc cggcgacgac gaccaggacc tcccagagga cgccgcccgg ggcgcggctc
     2401 gccacgagat cgcccatcgc gaagacgatg aaggagagga ccgcgtggga gagggcgaag
     2461 gccccgacgc ggaggaagga gaggctgttg gagaagtagt aggagaccga ctcgatgacc
     2521 tccacgagcc ccttgatggc gaaggccagg gcgccgtcgc ccttcccttc ccgcttccgg
     2581 ccctcgacga ggcggccgag cggctcctcc aggaagaggg cggccaaggg cagggcgaag
     2641 ccggggatgt ccggccagga gggccttccc cccgcggcga cgcggacgcc caagccgatc
     2701 gcccaccaga agaagaggga gcccgcgagg cccgtcttgg agaacagcgc ctcccccttc
     2761 ttcccgagcc tccacttgtt gacgatgttg acgacgaggc cgacggagtt gatgaggacg
     2821 ccgaccccga gcgtgaagcc gaagaaggcg aagagcttcg ccacgctgcc gccctcgggc
     2881 atgaccgtga cgaagcggtc gaggggccgc cccaggaggg cgagggagac ggcgcggctc
     2941 gcggggacga gccattcctc gttcgcgaag accgtgccgt ccaggaggcc catggccatg
     3001 gagcccaggc ccacggccgt gaagatgggc ccgtaggcct tccacttgcg gagcttcgcg
     3061 acctttcccc gtccgatgac gaggccggcc aggaggatga cggcgccctg gcccaggtcg
     3121 ccgaacatga tggcgaagag gacgacgaag aagaaggcga cgaggggggt cgggtcgatg
     3181 gtcccgtaga gcggcgcgcc gtaggagacg acgaggccct cgaaactgga gacgaagggg
     3241 cggcgcttga ggaggacggg gaccttctcc tcgcccgagc gcacggagtc gagctcctcg
     3301 gggtcataga ccctcgcggc ggcccggccc ccggtcgcgt cgccgagctc cttgaggagg
     3361 gccgcggccc tggccttggg cgcccagcct tcgaggcgat agacgaaatc ggaggactcg
     3421 agcccctcct tggcgcgctc gatggaggcg aggacggcgt aggaggccgc caggctctcc
     3481 cagctgtccc cgaccgaggc gcggagggcg gccttccggg cctcgagccg ggaggcctcg
     3541 gcgtccaggg cggcgcgctc ggcctccagg ctcgcgacga gctcgggagg caggcccttg
     3601 aagtcgggag gcagctccct gggccggaac ttggcgcgct cgagctcggt gtccagggcg
     3661 aagcggccct tcttggaggc ggcggcgacg acgctcccgt cctcgcccag ggggagtagg
     3721 atggcgcggt cgcccaggtt cgcggcgagg gcaggcagct cggagggggg gacgcggccg
     3781 acgcgcaccg cgaggaagga caggtggtcg agctccttga agggcaggga gagccccgcg
     3841 aaggcgcggg cctcctccag ggcctcgccg atcctgccgc gcctggcctg gagggcggcc
     3901 agctccgcgg agaggccttc cgccgcggcg acgagctcgc cgaggagggc gtcctcgcgg
     3961 gggcccggga gccgcgtgcc ctcgggaacc aggtccggcg cctcgaggtc gaggagatcc
     4021 ctgagcttgc ggagcttgtc gagggcggcc tcgacgcgcg gatcgcgccc tgggcgcggc
     4081 tcgccctccg agcgccgctg ctcggcgagc tcgaagcagc cggcccggcc caggatctcc
     4141 aggacgcggt ccatgtcgtt cttgaggatg aagagctcga gcttgcgcat ggctactggg
     4201 ctcacgcgac gcctcccgcc ggggccagcc aggcgatctc ctcggccggg gcgccgaggt
     4261 agatgccctc gaccaggccg aggacgacgg ccgcctcgaa ttccttgagc ttgaagaagc
     4321 agtagagcgg cgtgtaggtg aaggggtgcc ggtggagggc cctcctcagc ctgccgtaga
     4381 ggtagcgccg agcccgggct tcgacggtcc gcgggtcgat cccgcccgcc tggaagccct
     4441 ccgcgaggaa ggcgtacttc cagtcctcca agggggcgtc gcccctgggc ttcgcggccc
     4501 gccgcgccgc ccgcctcgcg gtccgcccct cggccccgcc ccccgcgccg ccgtccctgg
     4561 cgcccgcgag gcgggcccct tccagggccg cggagacgac gtcggccccg ggcagggcca
     4621 cgaggaggga ctcgatccgc ggaaggtcca tgccgtagta gcgcgcgagc ctcagggccc
     4681 agacgacgtt ctcgagctcc gcctcgaggg cgacgagctc gcgcagggaa ccgaggcggc
     4741 gggacgggac ggagcgcagg gcctcccaga gctcctcgta gaactgccgg tccaggcggt
     4801 tctccgcggc cggcaggtcc tccagggcct tgtccgagag ccagccgtag cggccgcccg
     4861 agaacatgga gccgagggag ggataggcgg actcgtcgaa gccggcggga tactcgggat
     4921 cgaaggactc ggggcaatgg agggcgcctt cgcggacggc gaggaggacg cgcttgacgc
     4981 ggccgaactc ggccttgcgc cgcagggcca cgaagaaggg ctcctcgcct cgcaggctgg
     5041 ccgcgagggc cacgaagtcg gcctgggcct cgcggaccgc gcgggactcg gccgcggcga
     5101 cgagggagcg ctcggggagg gcgggagggg cgtcgccgaa ggccgcgcgc cacgcgtccg
     5161 ccagccttcc gatcctgagg agctcggccg ccctgccgcc ggccagggat cccgccaggg
     5221 agccgcagac gcgggcgtag acataggcga gctcgtagcg gtcggccatg gcttctaggc
     5281 cttgaggtcc aggaatgcgt cgaacacggc gtcgaaggcg gcctcgtcct cgggggcgga
     5341 ggccagggcc tccctgtagg aggagatcag ggaggcgtac tcggcctcgc atgtcgccct
     5401 gccctcggcg aagccgcgct cgagctcggc ggctttccgt tcgtaggccc gcttgaaggc
     5461 ggtctccgcc tcttccttgg cggcggccac gcgccgcctg gcttcttccc tcgcctcgtc
     5521 gaggagggcg cgagcctcgc tctcgacggc gaagagcctg tccaacagtt cgcgagaagg
     5581 cattccagac ctccggagcg gctccccaag ggggccgctc agcgctggag ggtcaacaga
     5641 tcctcgaagc ttttaagaat ctcgaaggct cgctcgggcg tctccgccgc gacgagctcg
     5701 gtgtagaact cgggatcgtc aaggaggcgg gccaggcgct tcagggcgct caggtgcagc
     5761 tcggaatcct tggtggacga gatgagcatg aagacgaggt agacgggctc gccgtccagg
     5821 gagtcgtagt cgacgcccga cctggagacg ccgatggcgc ctacgagccc ctcgatcccc
     5881 tcggccttgc cgtgaggcag ggctatgccc ttcttgatgc ccgtggacat cttggcctcg
     5941 cgttcgcgca cggccaagag gacgtttcgg cgggggaagt cgcggccgcc cgatcgggcc
     6001 aggaggtcga cgagctcctc gaagagctcg tccttctcgc cgctctcaag gccgaccttg
     6061 atgcgggctg gcgtgaacac ttgctggagc agcatcggcg aacctcctcg gaccggcccc
     6121 gcggggggcc ggcccgacga tcacttgccg gcgggcgcgg gggcgggggc cgcgggcgcc
     6181 tggaggtcgc cgctcgcggg ggcgagctcg gcgggagcgg cgccctgggg ggcggcgttc
     6241 caccactcgg aggcggcggc gtcgcccttc ttctgtgcgg cggcttcgac gttgccgacc
     6301 gagcccttgt tgacgatggc caggctgaag gcgaggacga agaagaggcc gccgagcacg
     6361 taggtgacct tgatcaccac gttgctcgag cgggagccga aggcgctcga gctgccgccg
     6421 gcgaagatgc cgccgaggct ctcggagccc tcgtcctgca cgatgacgag gaggacgaga
     6481 aggatgctga cgatgacgaa gaccacgagg agaacgatgc cgagtatgcc catagtctgg
     6541 aactccacgc tgattgaagc ggcgcgaagc gtcctttctc ggggactcgc gcctataggc
     6601 ggcaatagta ccgaaaagcg ggatcgagtg caagaggagt cgagtcgcgt ggcggagctg
     6661 ctgatgccgg cccctgggca ggtcgtcgga cgggcggcgg ggcgatcggg accaggaagt
     6721 ccggcgaggc cacacgaaga cgctagatgc tggataattc catacccaac atatagttgg
     6781 caagcttggc cgcgactacg cgggcacagg gcatggcggc acccggcgga agcggcgccg
     6841 cggcgggctg cgcaccccgg cgaagcggcg ccgcggttcg gaaaaggacc ggccccggcc
     6901 gcgggctcgc cgcggccggg gccgcctggt ccgcgaacga cc
//
LOCUS       DAOOSJ010000144         6844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6863_length_6844_cov_6.310060, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000144 DAOOSJ010000000
VERSION     DAOOSJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_6844_cov_6.310060"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLG14_08030"
     CDS             complement(<1..536)
                     /locus_tag="PLG14_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX49118.1"
                     /translation="MQLLFAELAWILAFVAKAFLHILPYLALSLPLAVYLKRAGLAKK
                     IEGTLKGKEAVAVLVATLVGALSPFCSCGVIPIIAALLTGGAPLAPVMAFWLASPSMD
                     PEIFFLSVGSLGWELAVWRLVATFAMSLGAGYATLALERSGFLGTDILRGSAKAGKER
                     RATASPLRLFAPLGARLAA"
     gene            complement(607..939)
                     /locus_tag="PLG14_08035"
     CDS             complement(607..939)
                     /locus_tag="PLG14_08035"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOX49119.1"
                     /translation="MTNKKKAFGMPAACCSIEIDGDKQARFVAMLKAAGNPIRFEILK
                     FLVTHPGCITGDIVDHLPIAQATVSQHLKVLKEAGWIVGETEGPATCYQLDREGIAWF
                     RSSVNRLF"
     gene            complement(971..2014)
                     /locus_tag="PLG14_08040"
     CDS             complement(971..2014)
                     /locus_tag="PLG14_08040"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOX49120.1"
                     /translation="MAFRWFGRGDPVGLDRIRQIPCVTGIVSSLYEVPAGQAWDKAAL
                     AALKAEIEAAGLSFDCVESIPVHEDVKLGLPSRDRYIDAFVKGVEAMGELGIGVLCYN
                     FMPVFDWTRTQMSLSLPDGSTCLGYEDAALAGLLEGPGAFELPAWAKAYTKEELRSAL
                     EAYRGVSREDLWRNLEYFLAAVVPAAERAGVRLAIHPDDPPWDILGLPRVVVDGPALL
                     RLTASPRSPSNGVTFCTGSLGPEEGNDLPAMARSLASRIPFAHLRNVARTGTRDFHET
                     AHASSAGSLDMWEIVRALHESGFDGVVRPDHGRMIWGEQGRPGYGLYDRALGAMYLAG
                     LFEAAEKGTPPPA"
     gene            complement(2025..4331)
                     /locus_tag="PLG14_08045"
     CDS             complement(2025..4331)
                     /locus_tag="PLG14_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOX49121.1"
                     /translation="MKRHLPLRAVAALLVLLASGLPAFAQSKAAIVPSALSQELVKLF
                     PDLSSGYIDFSGNGKPDQTADLNEVVPESRVRDGQLQAQEILDFVVANWRFIPLERLR
                     AVQAAVKASPGAIGELIAIDYSASLGEAIARREEMGDGLYLTPSAYKEAMGKIGGIVS
                     AMAAAYKKEGQKAEADFVAGRDALFAMVDKGYPLPKDLPEEERAVLSTAMVGVMLKEK
                     GSSPARSRAAIRVLGQLKSAEAAPYLVELAAGTEYPIEAMRALAEIGYKPAIPVLAAQ
                     LRSAASPEVRKAALQATGAIGGAEGLDAVLEIAKPANREGVAPDLLEATAQALAGIAQ
                     KGNPDPRVQASLRELAGSDRPAIRKIAAGGIGAFATPQSAESLLLLVGNDKDVEVRKA
                     AVAVLGRQKAEGVMPALMKLLKEQDLDPALEAAAIQVVGEHPSGALAVQILVDALADR
                     DAGVRAAASAALKRLFPANQALVTGSLTRSLLSSQDEGFLVEGTALLASIADPSSLAT
                     LLALLQKPQSEVKRNAAWALYRIRSSANVKVVEELQKLVTNENESLAVRANAVRALGA
                     IGYDSPQLNVAGTLVTTAQMRGEKYAMLRYFAVRALGRLGPGKGQAAAALARIASRDP
                     DPELRKEAVSSLKELASASPEALESLAASYALAEDQELKVRVIEALADLGSDRPASLG
                     AEFLSGEAPAALKRRVVQAVSQAPSEASAAAILDAARDPAMADFAAAVLEGFPAPLIS
                     SIVARRLRTETDKGVLSVLASLDARFAE"
     gene            complement(4515..6755)
                     /locus_tag="PLG14_08050"
     CDS             complement(4515..6755)
                     /locus_tag="PLG14_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49122.1"
                     /translation="MMRRATRRAFDWRGPALASFVLFLLLGARGPAAQTLGPAAPAAS
                     EGRAAPAGRETGLDEYYRFPLSVGAQYRGLQALSGFATGFALRELGAELRAPLPFLPV
                     LQPLLRAGNTSYLEAGSSGGGKWDHDEWFASLGLGFSTRFSRNFELGLDAEGGLSQTY
                     FQNLAPGGATGSLGWLAGAGVRVGLIPSYNFAVDLRPSLRYSKSLGPLTEFDGLSFGV
                     GFAVSYRFGDDPDAPASVIRALRLLSGKLPPAFAAMQSYYASKPFATVELSNEEGYEL
                     KDLEVSFFQPGYMDAPTPVASLPSLPARGKAEVGLLAAFNQEVFKTQGSTPLAGELLV
                     RYRARGRSAEQRFPLSYELLDKTAIVWDDDRKVGAFVTPADSALRNYASYVRQSLKSE
                     ALPQLNTPLQTAAQVYAALGRLGLIYQADPSSPFVKAQVGQVSLDSVSLGRDTLVRGS
                     GDCDDLTVLFASLLESAGVETGFVTVPGHIYPAVNTKLKARDYRDLHPDRGMTLVVED
                     SLWIPLEITLVGKSGFVEAWRRGAELWRLYDADAAKRAFHKTAAAQELYRPVALRESD
                     LGLQYGSRDELLAAYRPELARLGDAALGELADSARKSGDKREWNALGVAYARYGRLKD
                     AEGAFQRALRADPAFASAQVNLGNLAFIQKEYRKALASYQAAANALQAQGKGSTQAAQ
                     VALVNVSMAYNALSSFAEARTAFERAAAIDPERVRDLAYLAQVGAGGAGRAAEQDSSV
                     LFLRDE"
BASE COUNT         1000 a         2190 c         2714 g          940 t
ORIGIN      
        1 gccgcgagcc gggcacccag gggggcgaag agcctgagcg gcgacgccgt ggcccgccgt
       61 tcctttccgg ccttggccga gccgcggagg atgtcggtgc ccaggaagcc gctccgctcc
      121 agggccaggg tcgcgtagcc ggcgcccagg ctcatcgcga aggtggccac gaggcgccag
      181 accgcgagct cccagccgag ggagccgacg gagaggaaga agatctccgg atccatggag
      241 ggcgaggcga gccagaaggc catcacgggg gcgaggggcg cgccgcccgt gaggagggcc
      301 gcgatgatcg ggatgacgcc gcaggagcag aagggactca gggcgccgac cagggtcgcg
      361 acgaggacgg cgacggcctc cttgcccttg agcgtcccct cgatcttctt cgcgaggccg
      421 gcgcgcttca ggtagaccgc gagcggcagc gagagggcga ggtagggcag gatgtggagg
      481 aaggccttcg cgacgaaggc gaggatccag gcgagctcgg cgaatagtag ttgcacggta
      541 cgctcctata acgtaaaaat acgataaaac aaacgaggga aaggacccgg ccgggcgctc
      601 cttcccctag aagagccggt tgacggaact acggaaccag gctatgccct cgcggtcgag
      661 ctggtagcag gtcgccggac cctcggtctc gccgacgatc cagcccgcct ccttgagcac
      721 cttgaggtgc tgggagacgg tggcctgggc gatgggaaga tggtcgacga tgtcgcccgt
      781 gatgcagccg gggtgggtga cgaggaactt gaggatctcg aagcggatgg ggttgcccgc
      841 cgccttgagc atggcgacga agcgggcctg cttgtcgccg tctatctcga tcgagcagca
      901 ggcggccggc atgccgaatg ccttcttttt attggtcata tcgcaataat acgatatagc
      961 ctcaggggag tcaagcgggc ggcggagtgc ccttttcggc cgcctcgaag aggcccgcga
     1021 ggtacatggc ccccagggcc cggtcgtaga ggccgtagcc gggccttccc tgctcgcccc
     1081 agatcatgcg gccgtggtcg ggtcggacga cgccgtcgaa gcccgactcg tggagggccc
     1141 ggacgatttc ccacatgtcg aggctgcccg ccgagcttgc gtgggccgtc tcgtggaagt
     1201 ccctcgtccc cgtgcgcgcg acgttgcgga ggtgggcgaa ggggatccgg ctcgcgaggg
     1261 agcgggccat cgcggggagg tcgtttccct cctcggggcc gagggagccc gtgcagaagg
     1321 tgacgccgtt ggaggggctc cgcggcgagg cggtcaggcg gaggagggca gggccgtcca
     1381 cgacgacgcg cgggaggcct aggatgtccc agggcgggtc gtccgggtgg atggccaagc
     1441 gcacgccggc gcgctcggcc gcggggacca cggccgcgag gaagtactcg aggttcctcc
     1501 acaggtcctc ccgcgagacg ccccggtagg cctcgagggc ggagcggagc tcctccttcg
     1561 tgtaggcctt ggcccaggcg gggagctcga aggcgcccgg cccctcgagg aggccggcca
     1621 gcgcggcgtc ctcgtagccg aggcaggtcg agccgtcggg cagggagagg gacatctggg
     1681 tcctggtcca gtcgaagacc ggcatgaagt tgtagcagag gacgccgatg ccgagttcgc
     1741 ccatcgcctc cacgcccttg acgaatgcgt cgatgtaccg gtcgcgcgac gggaggccga
     1801 gcttcacgtc ctcgtggacc gggatgctct ccacgcagtc gaaggagagg ccggcggcct
     1861 cgatctccgc cttgagggcc gcgagcgcgg ccttgtccca ggcctggccc gccgggacct
     1921 cgtagaggga agagacgatc cccgtcacgc aggggatctg cctgatccgg tccaggccca
     1981 cgggatcgcc gcggccgaac cacctgaagg ccatgcgcac ggggctactc cgcgaagcgg
     2041 gcgtccaggg aggcaaggac ggagaggacg cccttgtccg tctccgtcct gagccgccgc
     2101 gcgacgatgg acgagatgag cggcgccggg aagccctcca ggacggcggc ggcgaagtcg
     2161 gccatcgcgg ggtcgcgcgc ggcgtccagg atggcggccg cggaggcctc gctcggcgcc
     2221 tgggagacgg cctggaccac gcggcgcttg agcgcggcgg gggcttcgcc ggagaggaac
     2281 tcggcgccca gggaggcggg acggtcagag cccaggtccg ccagggcctc gatgacgcgg
     2341 accttgagct cctggtcctc ggccagggcg taggaggcgg ctagggactc gagggcctcc
     2401 ggagaggcgg aggcgagctc cttgagggag gagaccgcct ccttcctgag ctcggggtcg
     2461 gggtcgcggg aggcgatgcg ggccagggcg gccgcggcct gccccttccc tgggcccagg
     2521 cggcccaggg cgcgcaccgc gaagtagcgg agcatcgcgt acttctcgcc ccgcatctgc
     2581 gcggtcgtga ccagggtgcc ggccacgttg agctgggggc tgtcgtagcc gatcgcgccc
     2641 agggcgcgca ccgcgttcgc ccggactgcc aggctctcgt tctcgttcgt gacgagcttc
     2701 tgcagctcct ccaccacctt gacgttggcc gaactccgga tccggtagag ggcccaggcg
     2761 gcgttgcgtt tgacctcgga ctggggcttc tggaggaggg cgaggagagt cgccagggag
     2821 gaggggtcgg cgatggaggc gaggagggcc gtgccctcga cgaggaagcc ctcgtcctgg
     2881 ctcgagagga ggctccgcgt gaggctgccc gtgacgaggg cctggttcgc ggggaagagc
     2941 cgcttcaggg cggcgctcgc ggcggccctg acgcccgcgt cccggtcggc cagggcgtcg
     3001 accaggatct ggacggccag ggcgccggag ggatgctccc cgacgacctg gatggcggcg
     3061 gcctccaggg ccgggtcgag gtcctgctcc ttgaggagct tcatgagggc gggcatcacg
     3121 ccctcggcct tctgccgccc caggacggcg acggcggcct tgcgcacctc gacgtccttg
     3181 tcgttgccca cgaggaggag gaggctctcc gcggactggg gcgtcgcgaa ggcgccgatg
     3241 ccccccgctg cgatcttcct gatggcgggc cggtcggagc ccgcgagctc gcgcagggag
     3301 gcctggaccc gggggtcggg gttgcccttc tgggcgatgc ccgccagggc ctgggccgtc
     3361 gcctccagga ggtcgggggc gacgccctcg cggttcgcgg gcttcgcgat ctccaggacg
     3421 gcgtcgaggc cctcggcgcc gcctatcgcg ccggtcgcct ggagggcggc cttcctcacc
     3481 tcgggggagg cggcggagcg gagctgggcc gcgaggacgg ggatggcggg cttgtagccg
     3541 atctccgcga gggcgcgcat cgcctcgatg gggtactcgg tccccgcggc gagctcgacg
     3601 aggtagggcg cggcttccgc cgacttgagc tggccaagga cgcggatcgc cgcgcggctc
     3661 cgcgcggggc tcgagccctt ctccttgagc atgacgccga ccatcgcggt ggagaggacg
     3721 gcgcgctcct cctccggcag gtccttgggc agggggtagc ccttgtcgac catggcgaag
     3781 agggcgtcgc ggcccgcgac gaagtcggcc tcggccttct ggccctcctt cttgtaggcg
     3841 gcggccatgg cggagacgat gccgccgatc ttgcccatgg cttccttgta ggcggagggg
     3901 gtgaggtaga ggccgtcgcc catctcctcg cgcctggcga tcgcctcgcc cagggaggcg
     3961 gagtagtcga tggcgatgag ttcgcctatc gcgccggggc tcgccttgac cgcggcctgg
     4021 acggcgcgca gcctctccag ggggatgaag cgccagttcg cgacgacgaa gtcgaggatc
     4081 tcctgggcct ggagctggcc gtccctgacc ctggactcgg gcacgacctc gttcaggtcg
     4141 gccgtctggt cgggcttccc gttccccgag aagtcgatgt agccggagga gaggtcgggg
     4201 aagagcttca cgagctcctg ggagagggcc gaggggacga tcgccgcctt ggactgggcg
     4261 aaggcgggga ggccggaggc gaggaggacg aggagggcgg cgaccgcccg gagcggaaga
     4321 tggcgcttca tgggaggcat cgtagactat aggaggggga ctgtcaaagg cggccgcccg
     4381 ccctcgggcc gcccccggac ttccggaccg gcgggacggg gccgggcggc gcggccgcgg
     4441 gcaggcggcg cgaccgcggc caggcggtcg cgaagtcgcg gcgaggcggc cgggaggccg
     4501 gggcctcgat cccctcactc gtccctgagg aagagaaccg aactatcctg ctcagccgcc
     4561 cgccccgcgc cgccggcccc gacctgggcc aggtaggcca ggtcgcgcac ccgctcggga
     4621 tcgatggccg cggcccgctc gaaggccgtc ctggcctccg cgaagctcga gagggcgttg
     4681 taggccatcg agacgttgac cagggccacc tgggcggcct gggtcgagcc cttgccctgg
     4741 gcctggaggg cgttcgccgc ggcctggtag gaggcgaggg ccttcctgta ctccttctgg
     4801 atgaaggcga ggttgccgag gttgacctgg gcgctcgcga aggccggatc ggccctgagg
     4861 gcgcgctgga aggccccttc cgcgtccttg agtctgccgt agcgggcgta ggcgacgccc
     4921 agggcgttcc actcgcgctt gtcgccgctc ttcctggccg aatcggccag ctcgcccagg
     4981 gcggcgtcgc ccagcctcgc gagctcggga cggtaggcgg cgaggagctc gtccctggag
     5041 ccgtactgca ggcccaggtc cgactcgcgc agggcgacgg gccgatagag ctcctgggcg
     5101 gccgccgtct tgtggaaggc ccgcttggcc gcgtccgcat cgtagagacg ccagagctcg
     5161 gccccccgcc gccaggcttc gacgaagccg gacttcccga ccagggtgat ctcgaggggg
     5221 atccagaggc tgtcttccac gacgagggtc atgcccctgt cgggatggag gtcgcggtag
     5281 tcgcgggcct tgagcttcgt gttgaccgcg gggtagatgt ggcccggcac ggtgacgaag
     5341 cccgtctcca cgccggcgga ctcgaggagg gaggcgaaga ggacggtgag gtcgtcgcag
     5401 tcgcccgagc cccggaccag ggtgtcgcgg cccaggctca ccgagtcgag ggagacctgg
     5461 ccgacttgcg ccttcacgaa gggcgaggag ggatcggcct ggtagatgag gcccaggcgg
     5521 cccagggccg cgtagacctg ggccgcggtc tgcaggggcg tgttgagctg cggcagggcc
     5581 tcggacttga ggctttggcg cacgtagctc gcgtagttcc gcagggccga gtccgccggc
     5641 gtcacgaagg cgcccacctt ccggtcgtcg tcccagacga tggcggtctt gtcgaggagc
     5701 tcgtaggaga gggggaagcg ctgctcggcc gagcggccgc gggcgcggta gcgcacgagg
     5761 agctcgcccg cgaggggcgt cgagccctgg gtcttgaaga cctcctggtt gaaggccgcg
     5821 aggaggccga cctccgcctt tcccctcgcg ggcagggagg ggaggctcgc gacgggcgtc
     5881 ggcgcgtcca tgtagccggg ctggaagaag gagacctcga ggtccttgag ctcgtagccc
     5941 tcctcgttcg agagctcgac cgtcgcgaag ggcttcgagg cgtagtagct ctgcatggcc
     6001 gcgaaggcgg gagggagctt tccggagagg aggcgcaggg cgcggatcac ggaggcgggg
     6061 gcgtcggggt cgtcgccgaa gcggtaggag acggcgaagc cgacgccgaa ggagaggccg
     6121 tcgaactccg tgagggggcc gaggctcttc gaatagcgca gggacggccg caggtcgacc
     6181 gcgaagttgt aggaggggat gaggccgacg cggactccgg cgccggcgag ccagcccagg
     6241 gagcccgtcg ccccgcctgg cgcgaggttc tggaagtagg tctgcgagag gccgccctcc
     6301 gcgtccaggc cgagctcgaa gttgcgggag aagcgggtcg agaagccgag gcccagggag
     6361 gcgaaccact cgtcgtggtc ccacttgccg ccccccgagc tccccgcctc caggtagctc
     6421 gtgttgcccg cgcggagaag gggctggagg acgggcagga aggggagggg agccctgagc
     6481 tccgcgccga gctcgcgcag ggcgaagccg gtcgcgaagc ccgagagggc ctggaggccc
     6541 cggtactggg cgcctacgga gagggggaag cggtagtact cgtcgaggcc cgtctcgcgg
     6601 cctgccggcg cggcgcggcc ctccgaggcc gccggggcgg cggggccgag cgtctgggcc
     6661 gcggggccgc gggctccgag gaggaggaag aggacgaagg aggcgagggc cggtccgcgc
     6721 cagtcgaacg cacgccgtgt cgcgcgacgc atcattgggg acctcctcat ggcacgaggt
     6781 cgacgacgta gagcttggtc gctccgtcga gggcgtagag atgctcgccg tagagggcga
     6841 gcct
//
LOCUS       DAOOSJ010000145         6828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6879_length_6828_cov_5.005463, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000145 DAOOSJ010000000
VERSION     DAOOSJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6879_length_6828_cov_5.005463"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLG14_08055"
     CDS             complement(<1..600)
                     /locus_tag="PLG14_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49123.1"
                     /translation="MRIVKEAEERRNEILDAAEALFGEKGFAATSTGDILERIGIARG
                     TLYYHFESKAAIMEALVDRLAERLFRAARETAQATRDLPALDRLLATVAAFKLGGGSE
                     AGVMEHLHEPGNVLLHQRVQRAIVGGVTSILADIARDGVREGSFDTPYPQESMELVVV
                     YITEVIDGDLVELSDEARAARARALVFNLGRLFGLAEGDL"
     gene            complement(828..3077)
                     /locus_tag="PLG14_08060"
     CDS             complement(828..3077)
                     /locus_tag="PLG14_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49124.1"
                     /translation="MKKNLGIRGKLQLLFGPAMALILAVLVVFVNVRATSLVRELLKT
                     AGAETSARYATVIGSRMDNLFATARTLGQVVEGSGREPPARRRASVSGLLEGMLDKNQ
                     TLSSIWTTWEPDALDGLDGQNVGSAYGNDVGRFDATIYRGPDGSLRRKVSPEAEILES
                     EYYLEPKKTNQDTVVGPYLYSYEEGAEPLLETSVIVPIRAPDTTFRGVLGIDIPQSTF
                     QTIVAPIKPYGTGFAVLYAKGGAIAAHPDPSLVAKSLDDEAPLFPPADFELYKAAVLG
                     IDDASLFLSRDGARYLASIRKFRLGSSYTRWTLAVLVPEAKVLDGSRALTTRMAAAGV
                     AALFLVFLLVFAASRLVARPVRRVSAGLRDIAMGEGNLSLRLPATARDETGELAGYFN
                     DFVCKLEATVTKLKEVGRSGASIGDELAASSEESSATVTELDATVRSLQGKIASLDAS
                     IRSVEEAVGGISKGIDAMGGLVVRQSEAVVESKGASESIVEALGAMAKAAGERGAEAD
                     GLAERARAGEAVVGGVLGAVKDIGGYTARIAEMAQVINDVAERTNLLAMNAAIEAAHA
                     GDRGRGFAVVADEIRKLAEATGKNAATISQQLKTVTAKIDETSAGAEEAGASIRAMSE
                     GMGQAAASFREVLAGLTALAGRGAEAGRALDALVASTDELEAASADIDRRSDAIREET
                     LTLSRLSSENTAGFSEMAAGIREMRTASEELSRLGIENSRIATVMEEELGRFKTSEDL
                     CAGRPEGQE"
     gene            3209..4234
                     /gene="tdh"
                     /locus_tag="PLG14_08065"
     CDS             3209..4234
                     /gene="tdh"
                     /locus_tag="PLG14_08065"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOX49125.1"
                     /translation="MKALVKKSPEVGLWMEEAPMPQVGPKDILIKVKKTAICGTDVHI
                     YKWDAWSQRTIKTPMTIGHEFVGEIVETGKEVTGWKVGERVSAEGHIVCGECRACKAG
                     KRHLCPNTKGVGVNRTGCFAEFVSVPATNVWRVHPAVSDEVAAIFDPYGNATHTALSF
                     DMVGEDVLITGAGPIGCMAAAICRHNGARNVVITDVNDYRLSLAKKLGADRAVNVAQE
                     DLSKVMEELGMVGGFDVGLEMSGSPQAFAQMLDHMYNGGRIALLGIMPNGAGIDWDKV
                     IFKGLFLKGIYGREIFETWYKMEAMIRSGLDISPVITHRFKFDDFQKGFDAMLSGESG
                     KVVLSLD"
     gene            4386..>5034
                     /locus_tag="PLG14_08070"
     CDS             4386..>5034
                     /locus_tag="PLG14_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49126.1"
                     /translation="MRKHRSRSRQGTDDSRMESQGSGSAPGEPRPTPSTSGPASSPER
                     AEGSPGEASPRGASQGGAQGDDAAKKSRSRRRRERRKEAKSRGEGQPAEARPEQASAS
                     EAPAARPPRQKEARPPRRDEPRQREGQKPRPAAKAEDRPRGQQRRDEGKRPGKARRED
                     RPFRAEGRSRERPDQGRPDYARPDYAKPDYARPERRPRARPAGLPEGLPFDDELEL"
     assembly_gap    5035..5134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5135..6565
                     /locus_tag="PLG14_08075"
     CDS             <5135..6565
                     /locus_tag="PLG14_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49127.1"
                     /translation="ARPAGLPEGLPFDDELELPPGFRGPMGEPIGRPGGRGAFSKKAA
                     PAVGKLEALSPSSESLSIMERLEAVIEEAAPLQAKYRAGLKGDIRGLWEELTSDRESR
                     SVDYLGTPPALSAYLRYFMPWNVFRLSAIFSNADFCLPDNAVVVDIGSGPLTLPIALY
                     ASRPELREVPLTIYCMDRVERVLEAGKAIFETLCLRTSGRLPPWRIELKKEGFGGPLP
                     ERAHLLAAANVFNEFFWKDKRPLGERAIDTSRALLSYLRENGSLFVMEPGDPRSGAFI
                     SALRAALIAEGASPLGPCPHALSCPMPGIFRHLLPPEETRVVKGDPNAAPSRFVLPPV
                     VMPKKRDKFPWCHFGVDTDRAPAWLQALSQEAGLPKERAVMSYLWAARGAMARPPAGI
                     PEPMRSSEKERRARVGKGILVRVVSEPFALPEGATGQYACTNLGYTLLKRPRGEESFE
                     PGDLLEVATAPLQKYDEKSGAIVLPT"
     gene            6612..6686
                     /locus_tag="PLG14_08080"
     tRNA            6612..6686
                     /locus_tag="PLG14_08080"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6647..6649,aa:Asp,seq:gtc)
     gene            6719..6791
                     /locus_tag="PLG14_08085"
     tRNA            6719..6791
                     /locus_tag="PLG14_08085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6752..6754,aa:Pro,seq:cgg)
BASE COUNT         1038 a         2469 c         2225 g          996 t
ORIGIN      
        1 caggtcgcct tcggcgagcc cgaagaggcg gcccaggttg aagacgaggg cccgcgccct
       61 cgcggccctg gcctcgtccg acagctccac caggtccccg tcgatgacct cggtgatgta
      121 gaccacgacg agctccatgc tttcctgggg atagggggtg tcgaaggagc cctccctcac
      181 gccgtcgcgg gctatgtccg cgaggatcga cgtgactccg ccgacgatgg ccctctggac
      241 gcgctgatga aggaggacat tgcccggctc gtggagatgc tccatgacgc ccgcctccga
      301 cccgccgccg agcttgaagg ccgcgaccgt cgcgaggagc cggtcgagcg ccggcaggtc
      361 cctggtcgcc tgggccgtct ccctggccgc ccggaagagc cgctcggcga gcctatcgac
      421 cagggcctcc atgatggccg ccttggactc gaaatggtaa tagagcgtcc cgcgggctat
      481 cccgatccgc tccaggatgt ctcccgtgct ggtggccgcg aagcccttct caccgaaaag
      541 ggcctcggcc gcgtccagga tctcgttgcg gcgttcctcc gcttctttta ctattcgcat
      601 ggcctctctc gttaaccgac cgactgtctg tcggtagtaa tatcaagaag cccggcaacg
      661 agtcaagtgg agcccgcgga aaaaccgcga catcaggggg acggagcggc cgaaggcgag
      721 ggagacgagg gaggcgcgct ggcccggacc ggcctcacgc cggcccggac cggcctcacg
      781 ccagcccgga ctggtctctg ctccggccag cctccgcgca ggccgggcta ctcttggccc
      841 tctggcctcc cggcgcagag gtcttcgctc gtcttgaagc ggccgagctc ctcctccatc
      901 acggtcgcta tccgcgagtt ctcgatgccc agccgcgaca gctcctcgga ggcggtccgc
      961 atttcccgga ttccggcggc catctccgag aagccggcgg tgttctcgct cgagaggcgg
     1021 gagagggtca gggtctcctc ccggatcgcg tccgacctgc ggtcgatgtc cgcgctcgcc
     1081 gcctcgagct cgtcggtgga ggccacgagg gcgtccaggg cccttccggc ctcggcgccg
     1141 cgcccggcca gggcggtgag gcccgcgagg acctcccgga aactggccgc ggcctggccc
     1201 atgccctcgc tcatggccct gatggaggcg cccgcctcct ccgcgccggc ggaggtctcg
     1261 tcgatcttgg cggtcaccgt cttgagctgc tgggagatgg tcgccgcgtt cttgcccgtc
     1321 gcctccgcga gcttgcggat ctcgtcggcc acgaccgcga agccgcggcc gcggtcgccc
     1381 gcgtgggccg cctctatggc cgcgttcatg gcgaggaggt tggtgcgctc ggcgacgtcg
     1441 ttgatgacct gggccatctc cgcgatcctg gcggtgtagc cgccgatgtc cttcacggcc
     1501 cccaggaccc cgccgaccac ggcctccccc gccctggccc gctccgcgag gccgtcggcc
     1561 tcggccccgc gctcgccggc cgccttcgcc atggccccca gggcctcgac gatggactcg
     1621 gaggcgccct tcgactcgac gacggcctcc gactgccgga ccacgaggcc gcccatcgcg
     1681 tcgatgccct tggagatgcc gccgaccgcc tcctccacgc tcctgatgct ggcgtccagg
     1741 gaggcgatct tgccctgcag ggagcgcacc gtcgcgtcga gctcggtcac cgtcgcggag
     1801 gactcctccg agctggccgc gagctcgtcg ccgatggagg cgccgctgcg gcccacctcc
     1861 ttgagcttgg tcacggtggc ctcgagcttg cagacgaagt cgttgaagta gccggcgagc
     1921 tcgcccgtct cgtcccgcgc cgtcgcggga agcctgaggc tcaggttccc ttcccccatc
     1981 gcgatgtcgc ggaggcccgc ggagacgcgc ctcacgggcc gcgccacgag ccttgaggcg
     2041 gcgaagacga ggagaaagac gaggaagagg gccgcgacgc cggccgccgc catgcgggtg
     2101 gtcagggccc gcgagccgtc caggaccttc gcctcgggga ccaggacggc gagggtccag
     2161 cgggtgtagc tggagccgag cctgaacttc cggatagaag cgaggtagcg cgccccgtcc
     2221 ctggagagga agaggctcgc gtcgtcgatg ccgaggaccg cggccttgta gagctcgaag
     2281 tcggcggggg gaaagagcgg cgcctcgtcg tccaggctct tcgcgaccaa ggaagggtcg
     2341 ggatgcgcgg cgatggcgcc gcccttggcg tagaggacgg cgaagcccgt cccgtagggc
     2401 ttgatcggcg cgacgatggt ctggaaggtg gattggggga tgtcgatccc gaggacgccg
     2461 cggaaggtgg tgtcgggcgc cctgatgggc acgatcacgc tcgtctcgag gaggggctct
     2521 gcgccctcct cgtagctgta gaggtagggc cccacgaccg tgtcctggtt ggtcttcttg
     2581 ggctcgaggt agtactccga ttccaggatc tcggcctcgg ggctgacctt cctgcggagg
     2641 ctcccgtcgg gcccgcggta gatggtcgcg tcgaagcggc caacgtcgtt cccgtaggcg
     2701 ctcccgacgt tttggccgtc caggccgtcc agggcgtcgg gctcccaggt ggtccagatc
     2761 gagctcaagg tctggttctt gtccagcatg ccctcgagga gcccggagac cgaggcgcgg
     2821 cggcgggctg gcggctcccg tccgctgccc tccacgacct ggcccagggt cctggccgtg
     2881 gcgaagaggt tgtccatcct ggacccgatg acggtcgcgt agcgcgcgga ggtctcggcc
     2941 ccggccgtct tgaggagctc gcgcacgagg gaggtcgccc ggacgttcac gaagacgacg
     3001 aggacggcga ggatgagggc catcgccggc ccgaagagga gctgcagctt tcccctgatc
     3061 cccagattct tcttcacggc gaggccctcc tgaggctaca tgaccatcgt agagccatcc
     3121 ggggaaaagt caacggcccg gcaagcccgc ggccgtcctt cacagcccgc ggaaaccgga
     3181 ctatacttga cgtcaaaagg agaaccccat gaaagcgctc gtcaagaaaa gccccgaggt
     3241 gggtctctgg atggaagagg cgccgatgcc ccaggtcggc ccgaaggaca tcctcatcaa
     3301 ggtcaagaag accgccatct gcggcaccga cgtacatatc tacaagtggg acgcctggtc
     3361 ccagcgcacc atcaagaccc ccatgaccat cggccacgag ttcgtcggcg agatcgtcga
     3421 gacgggcaag gaggtgacgg gctggaaagt cggcgagcgc gtctccgccg agggccacat
     3481 cgtctgcggc gagtgccgcg cctgcaaggc cgggaagcgg cacctctgcc cgaacacgaa
     3541 gggcgtcggc gtcaaccgga ccggctgctt cgcggaattc gtctccgtgc ccgcgaccaa
     3601 cgtctggcgc gtccatcccg ccgtctcgga cgaagtcgcc gcgatcttcg acccctacgg
     3661 caacgcgacc cacaccgccc tctccttcga catggtcggc gaggacgtcc tcatcaccgg
     3721 cgccggcccc atcggctgca tggccgccgc gatctgccgc cacaacggcg cccgcaacgt
     3781 cgtcatcacc gacgtgaacg actaccgcct ctccctcgcg aagaagctcg gcgccgaccg
     3841 cgccgtcaac gtggcgcagg aggacctctc caaggtcatg gaggagctcg gcatggtcgg
     3901 cggcttcgac gtgggcctcg agatgtccgg cagcccccag gccttcgccc agatgctcga
     3961 ccacatgtac aacggcggcc gcatcgccct cctcggcatc atgcccaacg gcgccggcat
     4021 cgactgggac aaggtcatct tcaagggcct cttcctgaag ggcatctacg gccgcgagat
     4081 cttcgagacc tggtacaaga tggaagccat gatccgctcc ggcctcgaca tcagccccgt
     4141 catcacccac cgcttcaagt tcgacgactt ccagaaaggt tttgacgcga tgctgtccgg
     4201 agagtccgga aaagtcgtgt tgagtctcga ctgacaggaa tagccgcgtc aaaacctaag
     4261 gcggatcgac gaaggcgcaa gccttcgtcg ccgccgggcg aagcccggcg cgccgccgcg
     4321 gcggaggcct tttcgcgggg cccgtattgt ggcgagccgc ccggcggtgt tatagtggcc
     4381 ataccgtgag aaagcatcgc tccaggtccc gccaggggac cgacgattcg cggatggagt
     4441 cccagggctc cggatccgcg ccgggcgagc cgcgcccgac cccgtcaacc agtggccccg
     4501 ccagttcccc cgagagggcc gaaggctcgc cgggcgaagc ctcgcccagg ggagcttccc
     4561 agggaggagc ccagggcgac gacgcggcca agaagagccg ctcccggagg cggcgcgagc
     4621 gtcgcaagga agcgaagtcg cgaggcgagg gccagcccgc cgaggcgcgc cccgagcagg
     4681 cttccgcctc cgaggccccc gcggcgcggc cgcctcgcca gaaggaggcc cgccctcccc
     4741 gccgcgacga gccccggcag cgcgagggcc agaagccccg gccggccgct aaggccgagg
     4801 acaggccccg cggccaacag cgccgcgacg agggcaagcg gccgggaaag gcccgccgcg
     4861 aggacaggcc cttccgggcg gagggccgct cccgcgagcg ccccgaccag ggccgtcctg
     4921 actacgccag gcccgactac gccaagccag actacgccag gcccgagcgc cggccccggg
     4981 cccggccggc cggcctcccc gaaggcctgc ccttcgacga cgagctcgag ctgcnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccgg ccggccggcc tccccgaagg
     5161 cctgcccttc gacgacgagc tcgagctgcc tcccggcttc cgcggcccga tgggcgagcc
     5221 catcggccgt cccggcgggc gcggcgcctt ctccaagaag gcggcccccg ccgtcggaaa
     5281 gctcgaggcc ctctcgccgt cctccgaaag cctgtccatc atggagcggc tcgaggcggt
     5341 gatcgaggaa gccgcgcccc tccaggccaa gtaccgggcc ggcctcaagg gcgacatccg
     5401 cgggctctgg gaggaactga cctccgaccg cgagtcccgc tccgtcgact acctgggcac
     5461 gccgcccgcc ctttcggcct acctccgcta cttcatgccc tggaacgtct tcaggctctc
     5521 cgccatcttc tccaacgccg acttctgcct gcccgacaac gccgtcgtcg tcgacatcgg
     5581 ctcggggccc ctcaccctgc ccatcgccct ctacgcctcc cggccggagc tccgcgaagt
     5641 gcccctgacg atctactgca tggaccgcgt agagcgggtc ctggaggcgg gcaaggcgat
     5701 cttcgagacc ctctgcctga ggacgagcgg acgcctcccg ccctggcgca tcgagctcaa
     5761 gaaggaaggc ttcggcggcc ccctccccga gcgggcccac ctcctcgcgg cggcgaacgt
     5821 cttcaacgag ttcttctgga aggacaagcg ccccctgggc gagcgcgcga tcgacacctc
     5881 gcgggccctc ctctcctacc tccgcgagaa cggcagcctc ttcgtgatgg agcccggcga
     5941 tccccgctcg ggagccttca tctccgccct ccgcgcggcc ctcatcgccg aaggcgcctc
     6001 cccccttggc ccctgtcccc acgccctctc ctgccccatg ccggggatct tccgccacct
     6061 cctccccccg gaggagaccc gcgtcgtgaa gggcgacccg aacgccgcgc cctcccgctt
     6121 cgtcctcccg ccggtcgtga tgcccaagaa gcgcgacaag ttcccctggt gccacttcgg
     6181 cgtcgacacc gaccgcgccc ccgcctggct ccaggccctc tcccaggaag caggcctccc
     6241 caaggagcgg gcggtgatgt cctacctctg ggcggcgcgc ggggccatgg ccaggcctcc
     6301 cgccggcatc cccgaaccca tgaggagctc cgagaaggag cgccgcgccc gcgtcggcaa
     6361 gggcatcctg gtccgcgtcg tctccgagcc cttcgcgctt cccgagggcg ccacgggcca
     6421 atacgcctgc acgaacctgg gctataccct cctcaagcgg ccgcgcggag aggagtcctt
     6481 cgagccgggc gacctcctcg aggtggcgac cgcgcccctc cagaaatacg acgaaaaatc
     6541 cggggcgata gtccttccca cttgacatcg ggaaggctcc gccgatatag tgcccacgtc
     6601 gacaacgaca cgggggcgtg gcgaagctgg tttatcgcgc cggcctgtca agccggagat
     6661 cgcgggttca agccccgtcg ctcccgtccg acccgtctaa cgacgggtcg tttttttacg
     6721 ggctgtagcg cagtggctag cgcgcttggt tcgggaccaa gagatcgacg gttcaaatcc
     6781 gtccagcccg ataagcggtt accggaaacg gtgaccgctt tttcttta
//
LOCUS       DAOOSJ010000146         6819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6897_length_6819_cov_6.355115, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000146 DAOOSJ010000000
VERSION     DAOOSJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_6819_cov_6.355115"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLG14_08090"
     CDS             <1..419
                     /locus_tag="PLG14_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HOX49128.1"
                     /translation="GGIALQFPLYGGIQGIMVTTGLAGIIANWFISFSTAQTFPLFTF
                     LAGGLINFFVPSGGGQWVVQGPINIPAGIALGVDPAVVAMGISYGDQWTNMIQPFWAL
                     PLLGIAKLGIRDIMGYCMMTLLVSGAIIAAGLLILV"
     gene            complement(622..2259)
                     /locus_tag="PLG14_08095"
     CDS             complement(622..2259)
                     /locus_tag="PLG14_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOX49129.1"
                     /translation="MEHFGLLALIPPILVVVLAIWTKDVIISLFLGILSGTLIVAGGN
                     PYFALIKLTDILADMLADGWNIRIILFCGLLGALVGMWNKTGSARAFGAWAASKIKSR
                     TGVLIFTWIFGILIFIDDYFNSLTIGACMKPVCDEKKISRAKLAYILDSTAAPVCIIA
                     PISSWVITVMSYTKSSQGFDTLGVSEFTFFIRSIPYNLYAIFAILMVAFVAFKGRDFG
                     PMARSEARAKEGIGLFDEKKYGLVAGKMEASCEDLKAKWFDFVVPLVLLIASAVFFFP
                     MTTWMGAVDGEGVKTLSAAMQSMSLGEAFNNTDASKALFYSIIFTLVFSYVYFIARRL
                     LDIGKSAEAIVDGLKSMVPAIVILTMAWSIGFIIKKAPADGGVGLANFLSQVVVNGGF
                     PLWLLPLVVFLISCVISFATGTSWGTMAIMIPIALPIAVALGNKAGLAGDALINATLI
                     PVASVMGGAVFGDHCSPISDTTILSSTGANCPHLEHVVTQMPYAIFVAVCCAVGVVFA
                     GVFMSAVVGLAVTAALFVLGLIFLPKIWGEKKYDLQN"
     gene            complement(2401..3144)
                     /locus_tag="PLG14_08100"
     CDS             complement(2401..3144)
                     /locus_tag="PLG14_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOX49130.1"
                     /translation="MANIVRPYGDTLDDGKVQLSLTLPVPDGAKGREAARLMVLGWGF
                     KDCEIVHSSRISESFSFYVAYAKTESGIDYDSVVADEAASEDQVLDMEGVNAFVKEHI
                     GRKVVIVGACTGFDAHTVGIDAIMNMKGYNHHFGLERYSQLEAYNLGAQVPNEKLIEF
                     AEKVNADAILVSQIVTQKDIHIQNMTEFVELLEAKGERGRFVCVAGGPRISNKLAVEL
                     GYDVGFGKGSYAEHVATFVVRKVAERMKA"
     gene            complement(3158..4777)
                     /locus_tag="PLG14_08105"
     CDS             complement(3158..4777)
                     /locus_tag="PLG14_08105"
                     /inference="COORDINATES: protein motif:HMM:NF020610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOX49131.1"
                     /translation="MREKLGLPKDKIARARELAARSVAPVLDFVDGHTSVTVERAALR
                     IVGADGANADGVPVPNLVVDQLKDRLEGGAIVWYVNALLKSGGSVEDLNRRIAEGLRV
                     GDQPLGDREAVRRKALELVEAGLGRVRRNRAKRGEKMAEWKDRNGKPLIYLIVATGNI
                     YEDVKQAQAAAEKGADVVAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRAAL
                     DEVAEREKRYIWLTNYASGLCMPEIAAMGALERLDMMLNDSMYGILFRDINMYRTFVD
                     QKFSRMINSYAGITINTGEDNYLTTSDAYEKAYTVLASQFLNEQFGFAAGLPARLMGL
                     GHAFEMDPTIRNGFLYELAQAQMAREIFPEHPLKYMPPTKFMSGDIFKGLAMDTMFNF
                     VSKATNQGIHLLGMLTEAIHTPFMMDRDIAVENAKYVMNSMDSFYDEVEFKKDGIVVG
                     RAKEVLEQTLGFLGEVEDRGLLEAIEKGLFAEVKRPKDGGKGLDGLVKKGPEYWNPVE
                     EALKAGLGLPGRGRDYDATGAAAAASDGAKR"
     gene            complement(4794..6521)
                     /locus_tag="PLG14_08110"
     CDS             complement(4794..6521)
                     /locus_tag="PLG14_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49132.1"
                     /translation="MRSPSACSPAAWDFSRAEEFWERFSPLSPYGKDAKEERLVLSDQ
                     AAIEALYDGTERALAFLESRRDDPASLDRLSYHLRRLPRLPLERRGDAAYDLVEIFQF
                     KKFLSNYRAARLLLDEETRAWFGLGPEPAGLHAELAKGGSDAETFYVADDYDPRLRDV
                     RKSLARVDAAVAARRAAARARALAECGADFGGRDFLLVPRDAAQAILKDRALFAVEPY
                     DASSYIVRLQADDEELALETEREALRQEERVLEEGVLSGLSRLVAEELPRIEGLVAAI
                     RGFDLARARALLAVEEDLRRPLLREAPEGRLPALLVEGGRFLPCAWECGRLGLRYAPL
                     DLRLAESAAVLFGSNMGGKTVALESLVFFQVLAQAGFFTPAVRLETSVYPRIQYVGEL
                     KARSASRSALPAGGRGYVSEGLSGFGFEIRAFTEAWEEAGEGAFLVFDEFARTTSSRE
                     AEAILSAALEALARKPGVRALFSTHFRGVSRLPGVRYLRVRGLDRRAACEALGADEPL
                     GERIRRINRMMEYGLVDDEGPGPSGSDAVAIAALLGLDCGVVARAEDIYAAGMAGSDT
                     TRKAWTGKA"
     gene            complement(6514..>6819)
                     /locus_tag="PLG14_08115"
     CDS             complement(6514..>6819)
                     /locus_tag="PLG14_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49133.1"
                     /translation="AGLPVPAFALRGPLTAETLRQVPEAARTLIVEDFTKVFLDGPAL
                     EALLRSRSLAVESGIEFGGFVVILRDLSRERFRAALADPSLEALVAYNPYETEAASA"
BASE COUNT         1061 a         2222 c         2423 g         1113 t
ORIGIN      
        1 ccggcggcat cgccctccag ttccccctct acggcggcat ccagggcatc atggtgacca
       61 cgggcctcgc gggcatcatc gccaactggt tcatctcctt ctccaccgcc cagaccttcc
      121 ccctcttcac cttcctcgcc ggcggactca tcaacttctt cgtcccctcc ggcggcggcc
      181 agtgggtcgt ccagggcccg atcaacatcc ccgcgggcat cgccctcggc gtcgatcccg
      241 ccgtcgtggc gatgggcatc tcctacggcg accagtggac caacatgatc cagcccttct
      301 gggccctccc cctcctcggc atcgccaagc tcgggatcag ggacatcatg ggctactgca
      361 tgatgaccct cctcgtctcc ggcgcgatca tcgccgccgg cctcctcatc ctcgtctgat
      421 agcctgagtc atttcaccag gagagggccg cccggctggg cggccctctt ttttcccgcg
      481 gagccggctt ccgtggcccg aaacccgtaa gccccggaac ctccgtccag cccggccggc
      541 cagcgccgcg ggcggggccg ggcggataca aaaaaggccg cccatcgcgg ggcggccctg
      601 gcttcgtgac ggaaaagcct cttagttctg gaggtcgtac ttcttctcgc cccagatctt
      661 ggggaggaag atgaggccca ggacgaagag ggcggcggtg accgcgaggc cgaccaccgc
      721 gctcatgaac acgccggcga agacgacgcc gaccgcgcag cagacggcga cgaagatcgc
      781 gtagggcatc tgggtcacga cgtgctccag gtgggggcag ttcgcgcccg tggaggagag
      841 gatcgtcgtg tcggagatgg gcgagcagtg gtcgccgaag acggcgccgc ccatgacgga
      901 ggccacgggt atgagcgtcg cgttgatgag ggcgtcgccg gcgaggccgg ccttgttgcc
      961 cagggcgacg gcgatgggga gggcgatcgg gatcatgatc gccatcgtgc cccagctcgt
     1021 gccggtggcg aaggagatca cgcaggagat gaggaagacg acgaggggca ggagccagag
     1081 cgggaagccg ccgttcacga ccacctggga caggaagttg gcgaggccga cgccgccgtc
     1141 cgccggcgcc ttcttgatga tgaagccgat ggaccaggcc atggtcagga taacgatggc
     1201 gggcaccatg gacttgaggc cgtccacgat ggcctccgcg ctcttgccga tgtcgaggag
     1261 cctgcgggcg atgaagtaga cgtaggagaa gacgagggtg aagatgatcg agtagaagag
     1321 ggccttggag gcgtcggtgt tgttgaaggc ctcgccgagg ctcatcgact gcatcgcggc
     1381 ggagagggtc ttgacgccct cgccgtccac ggcgcccatc caggtggtca tcgggaagaa
     1441 gaagaccgcg gaggcgatga gaaggacgag gggcacgacg aagtcgaacc acttggcctt
     1501 gaggtcctcg caggaggctt ccatcttgcc ggcgacgagg ccgtacttct tctcgtcgaa
     1561 gaggccgatg ccctccttgg cgcgggcctc ggagcgggcc atcggtccga agtcccttcc
     1621 cttgaaggcg acgaaggcga ccatgaggat cgcgaagatc gcgtagaggt tgtaggggat
     1681 ggacctgatg aagaaggtga actcggagac gccgagggtg tcgaagccct gggagctctt
     1741 cgtgtaggac atcaccgtga tgacccagct ggagatcggg gcgatgatgc agacgggcgc
     1801 cgcggtggag tccaggatgt aggcgagctt ggcgcggctg atcttcttct cgtcgcagac
     1861 cggcttcatg caggcgccga tcgtgaggct gttgaagtag tcgtcgatga agatgaggat
     1921 gccgaagatc caggtgaaga tgaggacgcc ggtgcgggac ttgatcttgg acgcggccca
     1981 ggcgccgaat gcccgggcgg atccggtctt gttccacatg ccgaccaggg ccccgaggag
     2041 gccgcagaag aggatgatgc ggatgttcca gccgtcggcg agcatgtcgg ccaggatgtc
     2101 cgtcagcttg atcagggcga agtaggggtt gccgcccgct acgataaggg tgccagacag
     2161 gatgccgagg aagagggaga tgatgacgtc cttggtccag atcgcgagga cgacgaccag
     2221 tatcggcggg atgagcgcca atagcccgaa gtgttccatg taattcgcct ccttgatggt
     2281 gaatcccaac tcaacttagc agtttgcacc cgcccgggcg ggaatgcaac tgcttttttt
     2341 aaagaaaggg ccggcccccg tccgggagcc ggcctcgcct gcctcgcgcg ccgcggaggc
     2401 tcaggccttc atgcgctccg ccaccttgcg caccacgaag gtcgcgacgt gctcggcgta
     2461 ggatcccttg ccgaagccta cgtcgtagcc gagctccacg gcgagcttgt tggagatgcg
     2521 ggggccgccc gccacgcaga cgaagcgccc gcgctcgccc ttggcctcca ggagctcgac
     2581 gaactccgtc atgttctgga tgtggatgtc cttctgcgtg acgatctggg agaccaggat
     2641 ggcgtctgcg ttcaccttct ccgcgaactc gatgagcttc tcgttcggga cctgggcgcc
     2701 caggttgtag gcctcgagct gggagtagcg ctccagcccg aagtggtggt tgtagccctt
     2761 catgttcatg atcgcgtcga tgcccacggt gtgggcgtcg aagccggtgc aggcgccgac
     2821 gatcacgacc ttgcggccga tgtgctcctt cacgaaggcg ttcacgccct ccatgtcgag
     2881 cacctggtcc tcgctcgccg cctcgtcggc gacgacggag tcgtagtcga tcccggactc
     2941 ggtcttcgcg taggccacgt agaaggagaa actctcggag atgcgggagg agtggacgat
     3001 ctcgcagtcc ttgaagcccc agcccaggac catgagcctc gcggcctcgc ggcccttggc
     3061 tccgtcgggc acggggaggg tgagggaaag ctggaccttg ccgtcgtcga gcgtgtcgcc
     3121 gtagggccgt actatgttcg ccatggctct tcctccttta gcgcttcgcg ccgtcgctgg
     3181 cggccgcggc ggcccccgtc gcgtcgtagt cccggccgcg gccgggaagc ccgaggccgg
     3241 ccttcagggc ctcctccacc gggttccagt actcggggcc cttcttcacg agcccgtcca
     3301 ggcccttgcc gccgtccttg ggccgcttca cctcggcgaa gaggcccttc tcgatggcct
     3361 cgaggaggcc gcggtcctcg acctcgccca ggaagccgag ggtctgctcc agcacctcct
     3421 tcgcgcggcc gacgacgatg ccgtccttct tgaactccac ctcgtcgtag aaggagtcca
     3481 tggagttcat cacgtacttc gcgttctcca cggcgatgtc gcggtccatc atgaagggcg
     3541 tgtggatggc ctccgtgagc atgcccagga ggtggatgcc ctggttcgtg gccttggaca
     3601 cgaagttgaa catcgtgtcc atcgccaggc ccttgaagat gtcgcccgac atgaacttgg
     3661 tcgggggcat gtacttgagg gggtgctccg ggaagatctc gcgggccatc tgcgcctggg
     3721 cgagctcgta gaggaagccg ttgcggatcg tcgggtccat ctcgaaggcg tggcccaggc
     3781 ccatgagcct cgcggggagg ccggcagcga agccgaactg ctcgttcagg aactggctcg
     3841 cgaggaccgt gtaggccttc tcgtaggcgt cgctcgtcgt gaggtagttg tcctcgccgg
     3901 tgttgatcgt gatgcccgcg taggagttga tcatgcggct gaacttctgg tccacgaagg
     3961 tgcggtacat gttgatgtcg cggaacagga tgccgtacat ggagtcgttg agcatcatgt
     4021 ccaggcgctc cagggcgccc atcgcggcga tctccggcat gcacaggccc gacgcgtagt
     4081 tcgtgagcca gatgtagcgc ttctccctct ccgcgacctc gtccagggcg gcgcgcatga
     4141 tgcggaagtt ctcctgcgtc gcgtaggtgc cgccgaagcc ctccgtcgtg ggcccgtagg
     4201 ggacgtagtc gaggaggctc tgggccgtcg tcctgatgac ggcgacgacg tcggcgccct
     4261 tctccgcggc ggcctgggcc tgcttcacgt cctcgtagat gttgcccgtg gcgacgatga
     4321 ggtagatgag gggcttgccg ttgcggtcct tccactcggc catcttctcg ccgcgcttgg
     4381 cccggttccg cctgacgcgt cccaggcccg cctcgacgag ctccagggcc ttccgcctga
     4441 cggcctcgcg gtcgcccagc ggctggtcgc cgaccctgag gccctccgcg atcctgcggt
     4501 tcaggtcctc cacggagccg cccgacttga ggagggcgtt cacgtaccag acgatcgcgc
     4561 cgccctccag gcggtccttc agctggtcta cgacgaggtt ggggacgggg acgccgtccg
     4621 cgttcgcccc gtccgccccg acgatgcgga gggcggcgcg ctcgaccgtg accgaggtgt
     4681 ggccgtccac gaagtctagg acgggggcga cgctccgcgc cgcgagctcg cgggcgcgcg
     4741 cgatcttgtc cttgggcaat ccgagtttct ctctcatatg ctctccatct cctctaggct
     4801 tttcctgtcc atgccttgcg tgtcgtatcg gagccggcca tgccggccgc gtagatgtcc
     4861 tccgccctcg cgacgacgcc gcagtctagg ccgaggaggg ccgctatggc taccgcgtcg
     4921 ctgccggagg ggcccggccc ctcgtcgtcg acgaggccgt actccatcat cctgttgatg
     4981 cgcctgatcc gctcgcccag gggctcgtcc gcgcccaggg cctcgcaggc cgcccggcgg
     5041 tccaggcccc gcacccggag gtagcgcacg cccggcaggc gcgagacgcc gcggaagtgc
     5101 gtcgagaaga gggcgcggac gccgggcttc cgcgccaggg cctccagggc ggctgagagg
     5161 atggcctccg cctcccggga gctcgtcgtc ctcgcgaact cgtcgaagac caggaaggcg
     5221 ccctcccccg cctcctccca ggcctcggtg aaggcgcgga tctcgaagcc gaagccggag
     5281 agcccctcgg agacgtagcc cctgcccccg gccggcaggg ccgacctcga ggcggagcgg
     5341 gccttgagct cgccgacgta ctggatgcgg ggatagaccg aggtctctaa gcggaccgcc
     5401 ggcgtgaaga agccggcctg cgcgaggacc tggaagaaca cgaggctctc cagggcgacg
     5461 gtcttcccgc ccatgttgga gccgaagagg acggcggcgc tctccgcgag gcgcaggtcc
     5521 aggggggcgt agcggaggcc gaggcgcccg cactcccagg cgcaggggag gaagcgccct
     5581 ccctcgacca ggagggcggg aaggcgccct tccggcgcct cgcggaggag gggccgcctc
     5641 aagtcctcct cgaccgcgag gagggcgcgg gcccgggcca ggtcgaagcc gcgaatcgcg
     5701 gcgaccaggc cctcgatcct gggcagctcc tcggcgacga ggcgtgagag gcccgagagg
     5761 acgccctcct ccaggacccg ctcctcctgg cgcagggcct ctcgctccgt ctccagggcg
     5821 agctcctcgt cgtcggcctg gaggcggacg atgtaggagg aggcgtcgta gggctcgacc
     5881 gcgaagaggg ccctgtcctt gaggatggcc tgggcggcgt cgcggggcac gaggaggaag
     5941 tcgcggccgc cgaaatcggc gccgcactcg gccagggccc tggccctcgc ggccgcgcgc
     6001 cgggccgcga cggcggcgtc gacccgcgcc aggctcttcc gcacgtcgcg gagccgcggg
     6061 tcgtagtcgt cggcgacgta gaaggtctcc gcgtccgagc cgcccttggc gagctcggcg
     6121 tggaggccgg cgggctcggg gcccaggccg aaccaggccc tggtctcctc gtcgaggagg
     6181 agcctggccg cgcggtagtt cgagaggaac ttcttgaact ggaagatctc cacgaggtcg
     6241 taggccgcgt cgcccctccg ctcgaggggg agccgcggca ggcggcgcag gtggtaggag
     6301 aggcggtcca ggctcgcggg gtcatccctc ctcgactcga ggaaggcgag ggcccgctcc
     6361 gtgccgtcgt agagggcctc gatggccgcc tggtcggaga ggacgaggcg ctcctccttc
     6421 gcgtccttcc cgtagggaga gaggggcgag aagcgctccc agaactcctc cgcgcgggag
     6481 aagtcccagg ccgcggggga gcaggcggag gggctacgca ctcgcggcct ccgtttcgta
     6541 ggggttgtag gcgacgaggg cctcgaggga gggatcggcc agggcggcgc ggaagcgctc
     6601 cctggagagg tcgcggagga tgacgacgaa gccgccgaac tcgatgcccg actcgaccgc
     6661 gagggagcgg cttcggagaa gggcttcgag ggccggcccg tccaggaaga ccttcgtgaa
     6721 gtcctccacg atcagggtgc gggcggcctc ggggacctgg cggagggtct ccgccgtgag
     6781 gggcccgcgc agggcgaagg ccgggaccgg gaggcccgc
//
LOCUS       DAOOSJ010000147         6806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6914_length_6806_cov_6.810991, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000147 DAOOSJ010000000
VERSION     DAOOSJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6914_length_6806_cov_6.810991"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1305
                     /locus_tag="PLG14_08120"
     CDS             61..1305
                     /locus_tag="PLG14_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOX49134.1"
                     /translation="MQFKRSALALALALATAGSLFAQKTTVNFWTWRPEDTEAYNRLI
                     AVFEKANPDIDVVLSAQKNTEYNTILSAALSGGSGPDVFQSRAYGGLETFAQSGYMEP
                     LEKWVPELKGFDKAPLRGATSIKDGKVYGVPFASQTLFVYYNKALYKQLGLSVPETWD
                     QFLANLQAMKKAGVQPLANGGKDGWTLEVLMGAICPNFYGANDFFDAVVAGKTTFQDP
                     RYVGAIAKLKELTPYMPDLFMGVSYTDMQSSFINEMAGHFIGGSFEAGYFSAQNPKLE
                     YDVFPAPVAKKGDKPYVSVYADGNFSMNAASKNKDASAKFLRFLASKTTGDFFIKDLK
                     QVSAVPGADTSSSPYITKVLALQKYNTPYIMLVGFRYQQPTGSVLVQAALQGFLAGKF
                     DAAEVCKQVQEGVATWYAPFKK"
     gene            1379..2293
                     /locus_tag="PLG14_08125"
     CDS             1379..2293
                     /locus_tag="PLG14_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOX49135.1"
                     /translation="MTAKDLRKGLWIALFVLPGLVIVCTVILLPLFMSLFNSLFGWNQ
                     LLRGDFVGLDNFRKVFGTEPYKTRFLNALGNNLRWFAVTMAVQNTLGLLFGYLLSRRI
                     AGHEFFKRVIFIPVLFSIVAVGFLWGLYLRPNGLLNGFLKLAGRADLMRAWLGDERLA
                     TFSIILVNIWRWVGFPSLVFLAAIDNVPVECLEAAYLDGAREGTIFRRIILPLIVPSI
                     TVITVLTIIGSLNVFEQIYTMAGLDGPPNFSTDTIGTLFYRTAFGSVDSGSPEIGVGS
                     AIGVVIYLLTFVASILSIASLGRKEVEL"
     gene            2290..3144
                     /locus_tag="PLG14_08130"
     CDS             2290..3144
                     /locus_tag="PLG14_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOX49136.1"
                     /translation="MTTDFRFRGSGPGRKALIIAIEAAFIAYAALIFYPLANMVLSSF
                     KTTREILQKPFALPSRLDFSNYAVVWVEKGFGRYFLNSLWITAASMAFVLLFGSMAAY
                     GVSRYKYRLSTLVYMLFLSGIMLPLKAAIIPLFILMKNLRLIDKPLSVILVFIAMGLP
                     STVFILSGFMKAVPVDLEYAARIDGCSDWGIYRRIVMPVVAPAISLVTIYNAVPIWND
                     FFFPLVFLQSDRWKTLPVGLTSFFGQHSTNWSLLFTGLSIAVLPILFLYLFMAKYFIR
                     GMTSGAVK"
     gene            3158..4402
                     /locus_tag="PLG14_08135"
     CDS             3158..4402
                     /locus_tag="PLG14_08135"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOX49137.1"
                     /translation="MTKAEIESYRRLYEDELLGSVVPFWERHSPDREKGGYYSCLDRD
                     GSVFDTDKFLWMQGRGLWGFSTLCRKVAPEGRWLELARLGADFLREKGRAPNGDFWFS
                     VDREGRPLVQPYNIFSDCFCAAGLAEYGRASGEAWASELALAAYRRVLERKARPKGEW
                     TKQVEGSRPLLAMSMPMMEVWMAGEMAGLAPEDELEARVGAAVDQVFSLHVDRERKAV
                     FERVFADGGHADCMEGRLLTPGHALETLWFIMRAAAARGDAGRVEACREAMLWTAERG
                     WDGTYGGLYYYQDYEGRPTEKIESGMKLWWVHAEALCAFLLAYKLTGDPACEAWFRKT
                     HDWTWSRFRDPEYGEWFGYLDRRGEVALSLKGGKWKGFFHIPRALLECSRWLAQMAAA
                     CPEDGGYLPPFPAPGQARGGER"
     gene            4407..5366
                     /locus_tag="PLG14_08140"
     CDS             4407..5366
                     /locus_tag="PLG14_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOX49138.1"
                     /translation="MGAANSASAGSGRGLVFGLDGGGTSTRLRVADGDNRALYEGRGE
                     GINPNATAPDLVAGRLEALFAEAFRATGLAPGDFGAGCLGVAGADRPGERAELEAVLR
                     GRIGLSCPLEIASDPDIALVGGLRRLDGYLLIAGTGSIAIARLPDGARFRSGGLGHYL
                     GDEGSAFFIGFQAIRRALRSREGRDLPTSMLEPLMERFGLRDPSEFIALVYRRFDKAL
                     VASAAGLVERHRASGDELAAAIMAEARDELVALVASVRAQAGERIADPALVLWGGLME
                     HNAWLREGVAAGVARACPGMRVTEPLESAAYGACMLALGLARA"
     gene            5510..>6806
                     /locus_tag="PLG14_08145"
     CDS             5510..>6806
                     /locus_tag="PLG14_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49139.1"
                     /translation="MEMRSREDATRRNARFAFASFFVFMLLHQTDKLLIGPLQDQIME
                     SFKINYTQWGLVNTVAIVVGAVFYPLWGWLYDHYARPKLLALASLIWGCTTWLNAIAP
                     TYPVFLMTRASTGVDDSSYPGIFSLISDYYPPKKRGKMYGLLQVAQPLGYLVGMVLAI
                     VLGAAWGWRKIFYLTGGLGVLLAAVIFFRVKDQPRGAAEPELEGVQHLEKFKFSWKTV
                     GGLFRKKSLIILFLQGFIGVFPWQVITYYIFGYLGKNGRGFEGNEILFAMVPAILLMA
                     AGYPLGGVLGDRLFKRTPKGRVIVGAAGVALGAILLYAAIKVPYADKLGFGLLLAAAS
                     LFIPFASPNVISSFYDVTEPEIRATTNAAQNFIETAGSAAAPLLAGILADRTSVENAI
                     VVICGGAWALCFAFFVFAGRYIPKDIEDLKAKLKGRAADA"
BASE COUNT         1033 a         2576 c         2038 g         1159 t
ORIGIN      
        1 gactcggtgc taggctgtaa ccgtatttca atcatcgaca tcgcagccaa ggaggccctg
       61 atgcagttca agcgttcggc tctcgccctc gccctggccc tcgccaccgc gggctccctc
      121 ttcgcgcaga agacgaccgt caatttctgg acctggaggc cggaggacac cgaggcctac
      181 aacaggctca tcgccgtctt cgagaaggcg aatcccgaca tcgacgtcgt cctctccgcc
      241 cagaagaaca ccgaatacaa caccatcctc tccgccgccc tctccggcgg ctcgggcccc
      301 gacgtcttcc agagccgcgc ctacggcggc ctcgagacct tcgcccagtc cggctacatg
      361 gagcccctcg agaagtgggt gccggagctc aagggcttcg acaaggcgcc cctgcgcggc
      421 gcgacttcca tcaaggacgg gaaggtctac ggcgtgccct tcgcgagcca gaccctcttc
      481 gtctactaca acaaggccct ctacaagcag ctcggcctct ccgtgcccga gacctgggac
      541 cagttcctcg ccaacctcca ggccatgaag aaggccggcg tccagcccct cgccaacggc
      601 ggcaaggacg gctggaccct cgaggtcctc atgggcgcga tctgcccgaa cttctacggg
      661 gccaacgact tcttcgacgc cgtcgtcgcc ggcaagacca ccttccagga tccccgctac
      721 gtcggcgcca tcgcgaagct caaggagctc acgccctaca tgcccgacct cttcatgggc
      781 gtctcctaca ccgacatgca gtcctccttc atcaacgaga tggccggtca cttcatcggc
      841 ggcagcttcg aggccggcta cttctccgcg cagaacccca agctcgagta cgacgtcttc
      901 cccgcccccg tcgcgaagaa gggcgacaag ccctacgtct ccgtctacgc ggacggcaac
      961 ttcagcatga acgcggcctc caagaacaag gacgcctccg cgaagttcct ccgcttcctc
     1021 gcaagcaaga ccacgggcga cttcttcatc aaggacctga agcaggtctc cgccgtcccc
     1081 ggcgcggaca cctcgagctc cccctacatc acgaaggtcc tcgccctcca gaagtacaac
     1141 acgccctaca tcatgctcgt cggcttccgc taccagcagc ccaccggctc cgtcctcgtc
     1201 caggcggccc tccagggctt cctggccggg aagttcgacg ccgccgaggt ctgcaagcag
     1261 gtccaggaag gcgtcgccac ctggtacgcc cccttcaaga agtagatccc cgacgaaggc
     1321 cggggggagc ggaccgctcc ccccggcctt ccccatgccg cacataggag ccgccacgtt
     1381 gaccgcgaaa gacctgcgca agggcctctg gatagcgctc ttcgtcctcc cggggctggt
     1441 gatcgtctgc acggtcatcc tccttcccct cttcatgtcc ctcttcaaca gcctcttcgg
     1501 ctggaaccag cttctccgcg gcgacttcgt gggcctggac aacttccgca aggtcttcgg
     1561 caccgagccc tacaagaccc gcttcctcaa cgccctcggg aacaacctgc gctggttcgc
     1621 cgtcacgatg gcggtccaga acactctggg actcctcttc ggctatctcc tgtcaaggcg
     1681 gatagcgggc cacgagttct tcaagcgcgt gatcttcatc cccgtcctct tctcaatcgt
     1741 cgccgtcggc ttcctctggg gcctctatct ccggcccaac ggcctcctca acggcttcct
     1801 gaagctcgct ggccgggccg acctcatgcg ggcctggctc ggcgacgagc ggctcgccac
     1861 cttctccatc atcctcgtca acatctggcg ctgggtcggc ttcccgagcc tcgtcttcct
     1921 cgcggcgatc gacaacgtgc ccgtcgagtg cctggaggcg gcctacctgg acggggccag
     1981 ggaggggacg atcttccggc ggatcatcct ccccctcatc gtgccctcca tcaccgtgat
     2041 caccgtgctc acgatcatcg ggagcctgaa cgtcttcgag cagatctaca cgatggccgg
     2101 cctggacggg ccgcccaact tctccaccga cacgataggc acgctcttct accgcacggc
     2161 cttcggctcc gtcgactcgg gcagccccga gatcggcgtc ggctccgcga taggcgtcgt
     2221 catctatctc ctcaccttcg tagcgtccat cctgtccatc gcctccctcg gcaggaagga
     2281 ggtggagctg tgaccaccga cttccgcttc cgcggctcgg ggcccggcag gaaggccctc
     2341 atcatcgcca tcgaggcggc cttcatcgcc tacgccgccc tcatcttcta tcccctcgcc
     2401 aacatggtcc tctcctcctt caagaccacg agggagatac tccagaagcc cttcgccctc
     2461 ccctccaggc tcgacttctc caactacgcc gtggtctggg tggagaaggg cttcgggcgc
     2521 tacttcctca acagcctctg gatcacggcg gcctcgatgg ccttcgtcct cctcttcggc
     2581 tccatggcgg cctacggcgt ctcccgctac aagtaccgcc tgtccaccct cgtctacatg
     2641 ctcttcctct cggggatcat gctccccctc aaggcggcga tcatccccct cttcatcctg
     2701 atgaagaatc tccgcctcat cgacaagccg ctctccgtca tcctcgtctt catcgcgatg
     2761 ggcctgccgt ccacggtctt catcctctcg ggcttcatga aggcggtgcc ggtcgacctc
     2821 gagtacgcgg cgcgcatcga cggctgctcc gactggggca tctaccggcg catcgtcatg
     2881 cccgtcgtcg cccccgccat ctccctcgtg accatctaca acgcggtccc gatctggaac
     2941 gacttcttct tccccctcgt cttcctccag agcgacaggt ggaagaccct gcccgtgggg
     3001 ctcacgagct tcttcggcca gcacagcacc aactggagcc tcctcttcac gggcctctcc
     3061 atcgccgtcc ttcccatcct cttcctctac ctcttcatgg cgaagtactt catccgcgga
     3121 atgacgagcg gggcggtgaa atgagggggg cgggacaatg acgaaggcgg agatcgagtc
     3181 ctacaggcgg ctctacgagg acgagctcct cgggagcgtc gtccccttct gggagcgcca
     3241 cagccccgac cgcgagaagg gcggctacta cagctgcctg gaccgcgacg ggagcgtctt
     3301 cgacacggac aagttcctct ggatgcaggg ccgcgggctc tggggcttct ccaccctctg
     3361 caggaaggtg gcgccggaag gccgctggct cgagctcgcg cgcctggggg cggacttcct
     3421 ccgcgagaag ggccgcgccc cgaacggcga cttctggttc tccgtggaca gggagggcag
     3481 gcccctcgtc cagccctaca acatcttctc cgactgcttc tgcgcggccg gcctggccga
     3541 gtacggccgc gcctccggcg aggcctgggc ctccgagctc gctctcgcgg cctaccgccg
     3601 cgtcctggag cgcaaggcga ggcccaaggg cgagtggacc aagcaggtcg agggctcgcg
     3661 gcccctcctc gcgatgtcga tgccgatgat ggaggtctgg atggccggcg agatggccgg
     3721 cctcgccccc gaggacgagc tcgaggcccg ggtcggcgcg gccgtcgacc aggtcttctc
     3781 cctccacgtg gaccgggagc ggaaggcagt cttcgagcgc gtcttcgccg acgggggcca
     3841 cgcggactgc atggagggcc ggctcctcac gccgggccac gccctcgaga cgctctggtt
     3901 catcatgcgc gcggccgccg cgcgcggcga cgccgggcgc gtggaggcct gccgcgaggc
     3961 gatgctctgg accgccgagc ggggctggga cgggacctac ggcggcctct actactacca
     4021 ggactacgag gggcggccga ccgagaagat cgagtccggc atgaagctct ggtgggtcca
     4081 cgccgaggcc ctctgcgcct tcctcctcgc ctacaagctc accggggacc cggcctgcga
     4141 ggcctggttc aggaagaccc acgactggac ctggagccgc ttccgcgatc ccgagtacgg
     4201 cgagtggttc ggctacctgg accggagggg cgaggtcgcc ttgagcctca agggcgggaa
     4261 gtggaagggc ttcttccaca taccgcgcgc cctcctcgag tgctcgcgct ggctggccca
     4321 gatggcggcc gcctgtcccg aggacggcgg ctacctcccg cccttccccg cgccggggca
     4381 ggcgcgcggc ggggagcgtt aggcggatgg gcgccgctaa ctccgcctcc gccggctccg
     4441 ggcgcggcct cgtcttcggc ctggacggcg gcggcacctc gacccgcctc cgggtcgcgg
     4501 acggggacaa ccgggccctc tacgagggcc gcggggaggg catcaacccc aacgcgacgg
     4561 cccccgacct cgtcgccggc cgcctcgagg ccctcttcgc cgaggccttc cgcgcgacgg
     4621 gcctcgctcc cggcgatttc ggggccggct gcctaggcgt ggccggggcc gaccgccccg
     4681 gcgagcgggc ggagctcgag gcggtcctgc gcggcaggat aggcctctcc tgccccctcg
     4741 agatcgcctc cgatccggac atagccctcg tagggggctt aaggcgcctg gacggctacc
     4801 tcctcatcgc gggcacgggc tccatcgcga tcgcccgcct cccggacggc gcccgcttcc
     4861 gctccggcgg cctgggccac tacctgggcg acgagggctc ggcctttttc atcggcttcc
     4921 aggcgatacg gagggccctg cgctcccgcg agggccggga ccttcccacc tccatgctcg
     4981 agcccctcat ggagcgcttt ggcctccgcg atccctcgga gttcatcgcc cttgtctacc
     5041 gccgctttga caaggccctg gtcgcctccg cggccggact cgtggagcgg caccgcgcct
     5101 cgggcgacga gctcgcggcg gccatcatgg ccgaggcgcg ggacgagctc gtcgccctcg
     5161 tcgcctccgt ccgcgcccag gccggggaga ggatagccga tccggccctg gtcctctggg
     5221 gaggcctcat ggagcacaac gcctggctcc gcgaaggggt cgccgcggga gtggcccggg
     5281 cctgcccggg catgcgcgtg accgaaccgc ttgaaagcgc cgcctacgga gcctgcatgc
     5341 tcgccctcgg gctcgcgcgc gcgtgaaacg cgcttgcgcc cggaggcgct tgggcgtaca
     5401 gtaaggcgtc gaggaaaccg cggatcgcca cccggtgcgc gcggcctcgc ccaagcgcgg
     5461 cctcgtcctc gcgccgcaaa gaagtggtca gcggatgaaa ggaaacgaca tggaaatgcg
     5521 ctcccgggaa gacgcgacga ggcggaacgc gcgcttcgcc ttcgcctcct tcttcgtctt
     5581 catgctcctc caccagacgg acaagctcct catcgggccc ctgcaggacc agatcatgga
     5641 gtccttcaag atcaactaca cgcagtgggg cctcgtgaac accgtcgcca tcgtcgtcgg
     5701 cgcggtcttc tatcccctct ggggctggct ctacgaccac tacgcgaggc ccaagctcct
     5761 cgccctcgcc tcgctcatct ggggctgcac cacctggctc aacgcgatag cccccaccta
     5821 ccccgtcttc ctgatgaccc gcgcctccac gggcgtggac gactcgagct atcccggcat
     5881 cttcagcctc atctccgact actacccgcc caagaagcgg gggaagatgt acggcctcct
     5941 ccaggtcgcc cagcccctcg ggtacctcgt cggcatggtc ctcgccatcg tcctgggcgc
     6001 cgcctggggc tggcgcaaga tcttctacct gacgggcggc ctcggagtcc tcctcgcggc
     6061 cgtgatcttc ttccgcgtga aggaccagcc ccgcggcgcc gccgagcccg agctcgaggg
     6121 cgtccagcac ctcgagaagt tcaagttcag ctggaagacc gtcggcggcc tcttccggaa
     6181 gaagagcctc atcatcctct tcctccaggg cttcatcggc gtcttcccct ggcaggtgat
     6241 cacctactac atcttcggct acctgggaaa gaacggccgc ggcttcgagg ggaacgagat
     6301 cctcttcgcg atggtccccg ccatcctcct catggccgcg ggctaccccc tgggcggcgt
     6361 cctgggcgac aggctcttca agcggacgcc caagggccgc gtcatcgtcg gcgcggccgg
     6421 cgtcgccctg ggcgccatcc tcctctacgc cgcgatcaag gtcccctacg ccgacaagct
     6481 cggcttcggc ctcctcctcg ccgccgcctc cctcttcatc cccttcgcgt cgcccaacgt
     6541 catctcctcc ttctacgacg tcacggagcc ggagatccgc gcgacgacga acgccgcgca
     6601 gaacttcatc gagaccgcgg gctcggcggc cgcccccctc ctggcgggca tcctcgccga
     6661 caggacctcc gtggagaacg ccatcgtcgt catctgcgga ggcgcctggg ccctctgctt
     6721 cgccttcttc gtcttcgcgg gccgctacat cccgaaggac atcgaggacc tgaaggccaa
     6781 gctcaagggc cgcgcggcgg acgcgg
//
LOCUS       DAOOSJ010000148         6794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6930_length_6794_cov_5.082950, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000148 DAOOSJ010000000
VERSION     DAOOSJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_6794_cov_5.082950"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="lgt"
                     /locus_tag="PLG14_08150"
     CDS             complement(<1..478)
                     /gene="lgt"
                     /locus_tag="PLG14_08150"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOX49140.1"
                     /translation="MLAAVSFPAWLKPEIIPGLPFRWYGLMYVVAFAATWYLSNYESR
                     RRASPWTEDESTGFFFWAILGLLLGARLFGTLVYDATGYYWRKPWFVFWPFDEAGRFV
                     GFQGMSFHGGFVGVIVATLAYARSRRLSWLAWADVIAVSAPLGYTAGRLGNFINGEL"
     gene            complement(502..3588)
                     /locus_tag="PLG14_08155"
     CDS             complement(502..3588)
                     /locus_tag="PLG14_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOX49141.1"
                     /translation="MAQSIAKGVVALTVDELELEASLAFTPDPDGAEWTGEKILRLAM
                     DARISSLNQKRAEELVAKFARSRTPLSETIAKGQAPEAPRPEEAEWEELPFPEEWRSL
                     IEESLASAPPPELYAVRSEVVRVEKTVQKPAKLPFLRPKAEKVLVSEKRERRERVHPD
                     PSVLKTGYARKGERLALLSAAKAGKAGKSIFGRPLPAEQGEELAFLLGDGVERRKNEL
                     LAARDGVLRAGARWAELLPFAAPSYEVRKAPGGATFLLDYEPGDSRLPSPSPAEILAK
                     ARELGAPEGSLLPEAELAGLLAAAAAERKPLKARPLSLDRDGSAQVAISPDGLRATLT
                     LRKGRGKGKPLELSAVSAALKNAVPGGLKGVKAEELKKDVLEFYKSDRDELLDYLLVE
                     GKAPVRGKDRSLVLALEFLPEEEAAALRARLQGHPGLALAAPDLAEFPLDEAARLAFV
                     EEGQKIGSLSPGSPGQPGLDLFGRSIPGLPGNDPGIRTCDGALFAKGVVSAARAGLLA
                     ASERDGLWRFRVLPCRDASMEVSVSPDAMAAFLTLRPEEGLGEPLTVEGVLAALAEKG
                     VTSGQDAKAIGAAVVAARSGEAVLRRPVARGRPALPAGGLRLLWLARRPGEPPAPAAP
                     APGASGPAAQGAAAAAAPSAAALAALTAAEPPKGLRVAAGEAFLKAERRGAQGEPGLD
                     VLGRAVPAPPPAAASGGPSTEAGARAAAAAGAAVPLHDATVREEALPDGSTLLVAAVS
                     GELLVESGRVAVRDRLALDSDVGPLTGNVVFPGPVRVQGSVLAGFSLTAGGDALVGGS
                     VEAALVSSDGRIALSGGVKGARRGTLRARKTIEASFAEQSLLLAVEDIRIKSSVVLCN
                     AKTNGRFLVSDPKGAIVGGLTRARKGVEAAQIGSERGVKTEISFGQDYLVADQIEAEA
                     REIERLKALVVRADRTMAELERAAGLSGAGAAGLDPLRQDKVKLMKLIEKRSIRLFDL
                     REKFEEHHPSEVRVRGTIYPGVILESHNRFFEVRSKRTGVAFSFDPGTGRIVERPL"
     gene            complement(3599..4741)
                     /locus_tag="PLG14_08160"
     CDS             complement(3599..4741)
                     /locus_tag="PLG14_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOX49142.1"
                     /translation="MRIVPIASGKGGVGKSLVAANLSIALGQAGKRVVLADLDLGASN
                     LHLILGVPAPKAGIGTFLSGAAGKFENVVLDTDYENLRFVPGDAEIPGLANIKQSQKN
                     QLVKRLLSLDADYLVLDLGAGTSSNILDFFLMSSQGLVVTAPTLTATLNAYLFLKNAV
                     FRLLYASFGQRSKGYEYLESLRRDGASLQKAYIPAILEEVRKVDPERWAVAVERMSRF
                     RPRLLMNMLEDPKDAEKALKIRRSCKEYLNIEIEHLGVLYRDELQDIALGSRCPIIRY
                     KPQSVLSQGLYRAADKLVSMEGEADAPLSLAELEGSFQTAELEAESDFGAKMAYVEEL
                     LSSGALTTGDLVETVRSQQFEIDKLRRESQFLKYKLARAIGQGFSG"
     gene            complement(4743..5267)
                     /locus_tag="PLG14_08165"
     CDS             complement(4743..5267)
                     /locus_tag="PLG14_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49143.1"
                     /translation="MQRKPRFLLAALAEPIRFLGLAFLAGALGALRGGTASGALFRYA
                     AAPQLLFAAAFFFLWYDGERYAAYRPLAIVGKLLGLAALPVLLPGLLRAASEGPADPR
                     LVAVPAALVLLLDLLGLAALLLPAAKAPGSGGRAPGSSGAPGDGSGGGPSADNAPRAD
                     NVPRGPEDIERVEA"
     gene            5356..>6794
                     /locus_tag="PLG14_08170"
     CDS             5356..>6794
                     /locus_tag="PLG14_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOX49144.1"
                     /translation="MTANAQKPQRRRFAWGAVAAVLLLVSAIGFSAPAAFAEGSDSQA
                     AEAQRYFQIFENVYQFILQNYVDEVEAKKLYEGAMKGMFESLGDPYSVFLDEAMMSDM
                     NDTTEGKFGGIGLYISKQAVDPKAGEDAPRYIEIVSPIEDTPGWRAGFKPGDLIVKIE
                     GESTAPLSTDEAQGKLRGQAGKDITVTIRRGSAEFDITVTRAVIEIPTVKYALIPTKA
                     GNAAYLRIIEFTPQTLPRVQEAIKSFDAAGYSSVVVDVRSNPGGLLDSVVRIADLFLD
                     GGTIVSTKGRNPLENSVRRAKPDLAVPKDKPMVLLLNRGSASASEILAGALKDQKRAY
                     LVGENSYGKGSVQVPYPIDGTGFKLTLARYYTPSDENIDKTGIPPDLVAVEPELSEAE
                     TDALVRLFESGEIAAFAKQKPQAGQAEREAFAKALSAKGYALPERILRRLVRDEFQKT
                     SLAPVYDLEYDTALQAALAALADPAFPKRL"
BASE COUNT         1014 a         2291 c         2575 g          914 t
ORIGIN      
        1 agagctcgcc gttgatgaag ttgcccaggc ggcccgcggt gtagcccagg ggcgcggaga
       61 cggcgatgac gtcggcccag gcgagccagg agagcctgcg gctcctcgcg taggccaggg
      121 tcgcgacgat gacgccgacg aagccgccgt ggaaggacat gccctggaag ccgacgaagc
      181 ggccggcctc gtcgaagggc cagaacacga accagggctt gcgccagtag tagccggtcg
      241 cgtcgtagac cagggtgccg aagaggcggg cgcccaggag gaggccgagg atggcccaga
      301 agaagaagcc ggtggactcg tcctccgtcc agggcgaggc gcggcgcctg gactcgtagt
      361 tggagaggta ccaggtcgcg gcgaaggcga cgacgtacat gaggccgtac cagcggaagg
      421 gcaggccggg gatgatctcg ggcttgagcc aggcggggaa ggaaacggcg gcgagcatgg
      481 tcgctcctcg ttggtcagtc tttaaagcgg ccgctcgacg atgcggcccg ttccggggtc
      541 gaaggagaag gcgacgcccg tcctcttgct ccgcacctcg aagaagcggt tgtggctctc
      601 caggatgacg ccgggataga tcgtcccccg cacgcgcacc tcggaggggt ggtgctcctc
      661 gaacttctcg cgcaggtcga agagcctgat cgagcgcttc tctatgagct tcatcagttt
      721 taccttgtcc tggcgcagag ggtcgagccc cgcggcgccc gcccccgaga ggccggcggc
      781 gcgctcgagc tcggccatcg tgcggtcggc cctgacgacg agggccttga ggcgctcgat
      841 ctcgcgggcc tccgcctcta tctggtcggc gacgaggtag tcctggccga aggagatctc
      901 cgtcttcacg ccgcgctcgg agccgatctg cgcggcctcc acgcccttgc gggcccgcgt
      961 gaggccgccg acgatggcgc ccttggggtc ggagacgagg aagcggccgt tcgtcttcgc
     1021 gttgcacagg acgacggagc tcttgatcct gatgtcctcc acggcgagga ggagggactg
     1081 ctcggcgaag gaggcctcga tggtcttcct ggcccgcagg gtgccgcggc gcgcgccctt
     1141 cacgccgcct gagagcgcga tgcggccgtc gctggagacg agggccgcct ccacggagcc
     1201 gcccacgagg gcgtcgcccc cggccgtgag gctgaagccc gcgaggacgg agccctggac
     1261 gcggacgggc cccgggaaga cgacgttgcc cgtcaggggc cccacgtccg agtccagggc
     1321 caggcggtcg cggacggcga cgcggcccga ctcgacgagg agctcgcccg acacggcggc
     1381 gacgaggagg gtggagccgt cggggagggc ctcctcgcgg acggtggcgt cgtggagggg
     1441 gacggcagcg ccggcggcgg cggcggcgcg ggcgccggcc tccgtcgagg ggccgcccga
     1501 ggcggcggcg gggggcgggg cggggactgc ccgcccgagg acgtcgaggc ccggctcgcc
     1561 ctgggcgccg cgccgctccg ccttgaggaa ggcctcgccc gcggcgacgc ggaggccctt
     1621 ggggggctcc gcggcggtca gagcggcgag ggcggcggcg gaaggggcgg cggccgcggc
     1681 ggcgccttgc gcggccgggc cggaggcgcc gggggccggg gccgcagggg cggggggctc
     1741 gccggggcgg cgcgcgagcc agagtaggcg gaggccgccg gccgggaggg ccggccggcc
     1801 gcgggcgacg gggcggcgga gcacggcctc gcccgagcgg gcggccacga cggccgcgcc
     1861 gatggccttg gcgtcctggc cgctcgtgac gcccttctcc gccagggcgg cgaggacgcc
     1921 ctcgacggtc aggggctcgc ccaggccctc ctcgggacgc agggtgagga aggcggccat
     1981 cgcgtcgggg gagaccgaca cttccatgga ggcgtccctg caggggagga cccggaagcg
     2041 ccagaggccg tcccgctccg aggcggcgag gaggcccgcg cgggcggccg agacgacgcc
     2101 cttcgcgaag agggctccgt cgcaggtcct gatgcccggg tcgttgccgg gaaggccggg
     2161 tatggaccgg ccgaagaggt ccaggcccgg ctggccgggg gagcccgggc tcaaggagcc
     2221 gatcttctgg ccctcctcca cgaaggcgag cctggccgcc tcgtccaggg ggaattccgc
     2281 gaggtcgggc gccgccaggg ccaggccagg gtggccctgg agtcgggccc ggagcgccgc
     2341 ggcctcctcc tcgggcagga actcgagggc gaggacgagg ctcctgtcct tgccgcgcac
     2401 cggggccttt ccctcgacga ggaggtagtc gaggagctcg tcccggtcgc tcttgtagaa
     2461 ttcgaggacg tccttcttga gctcctcggc cttgacgccc ttgaggccgc cggggacggc
     2521 gttcttgagg gcggctgaga cggcggagag ctcgaggggc ttccccttgc ccctcccctt
     2581 gcgcagggtc agggtggcgc ggaggccatc gggggagatc gcgacctggg cgctcccgtc
     2641 gcggtccagg gagaggggcc gggccttgag gggcttcctt tccgccgccg cggccgcgag
     2701 gaggccggcg agttccgcct ccgggaggag ggatccctca ggcgcgccga gctcccgggc
     2761 cttcgcaagg atctccgcgg ggctcgggga ggggaggcgg ctgtcgccag gctcgtagtc
     2821 gaggaggaag gtggcgccgc ccggggcctt tctcacctcg taggaaggag cggcgaaggg
     2881 gaggagctcg gcccagcggg cgcccgcacg gaggacgccg tcgcgcgcgg ccaggagctc
     2941 gttcttccgg cgctcgacgc cgtcgccgag gaggaaggcc agctcctcgc cctgctccgc
     3001 ggggaggggg cggccgaaga tggacttgcc ggccttgccc gccttggccg cggagaggag
     3061 ggcgagcctt tcgcccttcc gcgcgtagcc ggtcttcagc accgaggggt ctgggtggac
     3121 gcgctcgcgc ctctcgcgct tttcggagac gaggaccttc tccgccttgg ggcggaggaa
     3181 gggcagcttc gcgggcttct ggacggtctt ctcgaccctg acgacctcgc tgcgcaccgc
     3241 gtagagctcg gggggagggg ctgaggcgag gctctcctcg atgaggctcc gccactcctc
     3301 ggggaagggc agctcctccc actcggcctc ctcgggccgg ggagcctcgg gggcctggcc
     3361 cttcgcgatg gtctccgaga ggggcgtgcg ggagcgggcg aacttggcga cgagctcctc
     3421 ggcccgcttc tggttgaggc tggagatccg cgcgtccatg gcgagtcgga ggatcttctc
     3481 ccccgtccat tcggctccgt cgggatcggg cgtgaaagcc agggaggcct cgagctcgag
     3541 ctcgtcgacg gtgagggcga cgacgccctt cgcgatgctc tgcgccatga ccgccgtcct
     3601 agcccgagaa tccctgcccg atggccctcg cgagcttgta cttcaggaac tggctctcgc
     3661 ggcgcagctt gtcgatctcg aactgctggc tgcgcaccgt ctccacgagg tcgcccgtcg
     3721 tgagggcgcc ggaggagagg agctcctcga cataggccat cttcgcgccg aagtccgact
     3781 ccgcctcgag ctcggcggtc tggaagctcc cctcaagctc cgcgagggag aggggcgcgt
     3841 cggcctcccc ttccatggac acgagcttgt ccgcggcgcg gtagaggccc tgcgagagga
     3901 cggactgggg cttgtagcgg atgatcgggc agcggctccc cagggctatg tcctggagct
     3961 cgtcgcggta caggacgccc aggtgctcga tctcgatgtt gaggtactcc ttgcaggagc
     4021 ggcggatctt cagggccttc tccgcgtcct tcgggtcctc cagcatgttc atgaggaggc
     4081 gggggcggaa gcgcgacatg cgctcgaccg cgacggccca gcgctcgggg tcgaccttcc
     4141 gcacctcctc caggatcgcg gggatgtagg ccttctggag ggaggcgccg tcgcggcgga
     4201 gcgactccag gtactcgtag cccttggagc gctggccgaa ggaggcgtag aggaggcgga
     4261 agacggcgtt cttgaggaag aggtaggcgt tgagcgtcgc cgtgagggtc ggcgccgtga
     4321 cgaccaggcc ctgggagctc atgaggaaga agtcgaggat gttcgagctc gtgcccgcgc
     4381 ccaggtccag gacgaggtag tccgcgtcca gggagaggag gcgcttcacg agctggttct
     4441 tctggctctg cttgatgttc gcgaggcccg ggatctccgc gtcgccgggg acgaagcgga
     4501 ggttctcgta gtccgtgtcg aggacgacgt tctcgaactt gcccgccgcc ccggagagga
     4561 aggtgccgat gcccgccttg ggggccggca cgccgaggat caggtggagg ttcgaggccc
     4621 cgaggtccag gtcggcgagg acgacccgct tcccggcctg gcccagggct atggagaggt
     4681 tcgcggcgac gagggacttg ccgacgccgc ccttgccgct cgcgatgggg acgatgcgca
     4741 agctaggcct ccaccctttc gatgtcctcg ggcccgcgcg gaacgttgtc cgcgcgcggg
     4801 gcgttgtccg cgctcgggcc gccgccggag ccgtcccccg gcgcgccgga ggagcccggg
     4861 gcccggccgc ccgatcccgg ggccttcgcc gcgggaagaa ggagggctgc gaggccgagg
     4921 aggtcgagga ggaggaccag ggcggccggg acggcgacga ggcgggggtc ggccgggccc
     4981 tcggaggccg cccggaggag gccggggagg aggacgggca gggccgcgag gccgaggagc
     5041 ttcccgacta tggccagggg cctataggcg gcgtagcgct ccccgtcgta ccagaggaaa
     5101 aaaaaagccg ccgcgaagag gagctgggga gccgcggcgt agcggaagag ggcgccggag
     5161 gccgtccctc cgcgcagggc gcccaaggcc cccgcgagga aggcgaggcc gaggaatctg
     5221 atcggttccg ccagggcggc gaggaggaag cggggctttc gctgcatata cagcgggaag
     5281 tgtacgagga ggccccccgg cagtcaaggg atcgaggagt tgacaggggc cgccgcggga
     5341 aaggatagtg aaagcatgac agcgaacgcc cagaaaccgc aacgccgccg cttcgcctgg
     5401 ggcgcggtcg cggccgtcct cctcctcgtc tccgcgatcg gcttctccgc gccggccgcc
     5461 ttcgccgagg gaagcgactc gcaggccgcc gaggcccagc gctatttcca gatcttcgag
     5521 aacgtctacc agttcatcct ccagaactac gtcgacgagg tcgaggcgaa gaagctctac
     5581 gaaggggcga tgaaggggat gttcgagtcc ctgggcgatc cctactccgt cttcctggac
     5641 gaagccatga tgagcgacat gaacgacacc acggagggaa agttcggcgg catcggcctg
     5701 tatatcagca agcaggccgt cgatcccaag gccggcgagg acgccccccg ctacatcgag
     5761 atcgtctccc ccatcgagga cacgccgggc tggcgggccg gcttcaagcc gggcgacctc
     5821 atcgtgaaga tcgaggggga gagcaccgcc cccctctcca cggacgaggc ccagggcaag
     5881 ctccgcggcc aggcgggcaa ggacatcacg gtgacgatcc gccggggaag cgccgagttc
     5941 gacatcacgg tgacgcgcgc cgtcatcgag atcccgaccg tgaagtacgc cctcatcccc
     6001 accaaggcgg gcaacgcggc ctacctccgc atcatcgagt tcacccccca gaccctcccc
     6061 cgcgtccagg aggccatcaa gagtttcgac gcggccggct actcctccgt cgtcgtcgac
     6121 gtccgctcca acccgggcgg cctcctcgac tccgtcgtgc gcatcgcgga cctcttcctg
     6181 gacgggggca cgatcgtctc cacgaagggc cgcaatcccc tcgagaattc cgtcaggcgg
     6241 gccaagcccg acctcgccgt gcccaaggac aagccgatgg tcctcctcct gaaccgcggc
     6301 tcggcctccg cctcggagat cctcgcgggc gccctcaagg accagaagcg ggcctacctg
     6361 gtcggcgaga acagctacgg caagggctcc gtccaggtgc cctaccccat cgacggcacg
     6421 ggcttcaagc tcaccctcgc gcgctactac acgcccagcg acgagaacat cgacaagacg
     6481 ggcatcccgc ccgacctggt cgccgtcgag cccgagctct ccgaagccga gaccgacgcc
     6541 ctcgtgcggc tcttcgagag cggcgagatc gccgccttcg cgaaacaaaa gccccaggcg
     6601 ggccaggccg agcgcgaggc cttcgccaag gcgctctcgg ccaagggcta cgccctcccc
     6661 gagcgcatcc tcaggcgact cgtccgcgac gagttccaga agaccagcct cgcgcccgtg
     6721 tacgacctcg agtacgacac ggccctccag gccgccctgg ccgccctcgc cgaccccgcc
     6781 ttcccgaagc gcct
//
LOCUS       DAOOSJ010000149         6778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6957_length_6778_cov_6.094006, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000149 DAOOSJ010000000
VERSION     DAOOSJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6957_length_6778_cov_6.094006"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLG14_08175"
     CDS             <1..488
                     /locus_tag="PLG14_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOX49145.1"
                     /translation="MATRRLLSFLEQNKVGYRLATHRTAYSAMRTAEEAHVKGAEMAK
                     SVALMVDGNPMLAVVPATMSVSFKRMKELLGAREVSLLDERKLDEYFPDCETGAMPPV
                     GAMYHMEVVADSELEYDDRISFNAGTHKEIVTMNFQDWRRLVHPRFAAIHRSGLGELT
                     I"
     gene            complement(758..831)
                     /locus_tag="PLG14_08180"
     tRNA            complement(758..831)
                     /locus_tag="PLG14_08180"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(795..797),aa:Val,seq:tac)
     gene            976..1935
                     /locus_tag="PLG14_08185"
     CDS             976..1935
                     /locus_tag="PLG14_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOX49146.1"
                     /translation="MEIVGIGNALMDVIAFVDEDFAPSRGFHNNATVHLDRARLDAVL
                     AGLPEAVFAAGGGAANTVRAAALLGLKASFSGSVGEDELGLRYRGELEEAGVEPLLSA
                     SPAPTGVYCALVRPDGGRSLLVAPSAALDLVLEPPPQAMFKAGAILYLEGFLLRDRAF
                     FMACLEGGRRAGMKIALDLGSFSLVRAQRDFLLELLPDYVDYLFANEDEFSALAELPF
                     GEAVELLADAGPAIVVKLAERGAVYARGGERIESPVRALRPVDETGAGDAFAAGFLAG
                     IARGYAPERCLRLGNRIAEEVLGVPGLAVDSERLKAAWESVSA"
     gene            2124..4472
                     /gene="lon"
                     /locus_tag="PLG14_08190"
     CDS             2124..4472
                     /gene="lon"
                     /locus_tag="PLG14_08190"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOX49147.1"
                     /translation="MPETEIIPIEQILPNKLPIIPLMGRPIFPGIFTPIMVGNPDDVK
                     VVEEALAADGMIGLVMIKNEFDKPKSDDLHAVGTAAKIVKRINLPDGGVNIFISTMKR
                     FKIKKFLSKETPIVAAVGYLDEEDDDSDEVKALTRALLGEMKQISENNPLFSEEMRLN
                     MINIDHPGKIADFIASILNIDKNDQQKILETTNVRDRMEKVLIFIKKEQELLKIQKRV
                     QQEINEKIEKSQREYFLKEELKAIKGELGMPTDAKGSDYQRFKEKIESFGFEGEVKEQ
                     VEQELEKFNLMDANSSEFIVTRNWLDLICNLPWKAPVSGDFDLSRAQEILEADHYGLK
                     DVKDRIVEYLAVRKLKKDAKGSILCLVGPPGVGKTSVGRSIARALNKQFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIVQGLKITKSRDPVFMIDEIDKMGSSFQGDPSSALLETL
                     DPEQNFSFRDHYLDLPFDMSNVFFICTANTLDSIPGPLVDRMEIIQLPGYIDSEKLEI
                     AKHYLVPKSLEKNGIRKNQVRYTRDALMFIAESYAREAGVRNFEKALDKIHRKLAKEI
                     VLEGKEGDKFPVDKAAVQKQLGKPLFREDDIKKATGPGMSIGLAWTSMGGDTLVIEAV
                     ANPGKEGFKLTGQMGSVMQESAGIAYTHVRHIASERYGVGADWFEGRQVHLHIPEGAT
                     PKDGPSAGITMATALLSLVLGRRIKDRLAMTGELSLTGQVLPIGGLREKTVAAKRNRI
                     REIVIPKANEKDLDDIPENVKKGLTFYPVERFEEVVAIALPD"
     gene            4621..6240
                     /locus_tag="PLG14_08195"
     CDS             4621..6240
                     /locus_tag="PLG14_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49148.1"
                     /translation="MNLALNKSVSGEVRELEIRKKTPILRAFVLALVILLPIVAASDL
                     VTGDTLFGLIEVFLFAVFVASFFLLRAGKYLLVSRFASILVWLAMAFISYNGRADGTS
                     SVYRVVIFLATAVAFSSFFLLDSLVPIVLAAANALVIGAYSFLRLAGSMSAGETLNNV
                     IVSQLFNALVLFLVIQPTRLGRSISEELEAERGRGASRLATLNRAAAGSEENLRSIGA
                     LAGRVEEIRSAVETASKAAARIESGLARIDRAADAATAEAASIGDRMRELNGHIEAEA
                     SAQVESAASVNEMVASIGSVADSARKRRDGMRDLVGTTEEGQARLSSLLGMIGRVEGS
                     VDAIRQMVTVINKIASSTNLLAMNASIEAAHAGEAGKGFSVVAEEIRGLAENSARNAK
                     EIGQRLKDVIQAITEAAGQSGLMKESFGKIREEIDAAISSFDEIKEATGELSEGGRQI
                     LESLKVLNETSTGVREGGALILGAQERLLGLQGQAKDAVRELAGEVGVVAERNQALLS
                     SALAVSEVAAEGQRRAEELHATMNGADGASA"
     gene            complement(6264..>6778)
                     /locus_tag="PLG14_08200"
     CDS             complement(6264..>6778)
                     /locus_tag="PLG14_08200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOX49149.1"
                     /translation="AILPLGVFALLGARLPGLEYLYAGDPRAWAMPAAALALVAYAAG
                     RLGPMDKADLANYPQYLPARWGPGELLAEGLSWLLYLSAYEFVFRGMLLSVLMPAGAL
                     AAAAASTALYAFAHLPKGNKEALGCLVFGVAASALTLRFGSVLPAALVHTALALGNDR
                     NAFVRLSGGS"
BASE COUNT         1203 a         2348 c         2227 g         1000 t
ORIGIN      
        1 ccatggctac gaggcggcta ttgtccttcc tcgagcagaa caaggtgggc tatcggctgg
       61 ccacgcaccg gaccgcctac tcggcgatgc ggacggcgga ggaggcccac gtcaagggcg
      121 cggagatggc gaagagcgtc gccctcatgg tggacgggaa tcccatgctc gcggtcgtgc
      181 ccgccaccat gtccgtctcc ttcaagcgca tgaaggagct tttgggcgcg agggaggtct
      241 ccctcctcga cgagcggaag ctcgacgagt acttccccga ctgcgagacg ggcgcgatgc
      301 cgccggtggg cgccatgtac cacatggagg tcgtggccga ctcggagctc gagtacgacg
      361 accgcatctc cttcaacgcg ggcacgcaca aggagatcgt gacgatgaac ttccaggact
      421 ggcgccgcct ggtccacccg cgcttcgccg ccatccaccg ctccgggctc ggggagctca
      481 cgatctaagc ccggcgctcc ggttcgattc cctcagcgca ccaaaaactg aagaccgctt
      541 tcgcggtctt ctctgtctgg gcgctgaggg aatcggcctc cggccctcga cttcctgtct
      601 cgggcctccg ggccgctctc ggcgtctccc ggcgcatcca tgcgccgggt tcttgtccga
      661 cgctcatcta ttcgcgtcgg acaagaaccg acgccttcgg ttcgattccc tcagcgcaat
      721 aaaatgcgaa ggccgcctat cggcggcctt cattcaatgg gcgctgaggg aatcgaaccc
      781 ccgacctact cggtgtaagc gagccgctct aaccagctgg gctaagcgcc ccaggcccgg
      841 aaggctaaca cgggcccgct cggggggtca agcggacgcg gttccgcggc cggcccgcgg
      901 tcctttcccg ccttgcccct ccttcgggac gatgctatca tgaggcgtcg gacagtcccg
      961 tcgcgaggag ggcccatgga gatcgtcggc atcggcaacg cgctcatgga cgtgatagcc
     1021 ttcgtcgacg aggatttcgc tccctcccgc ggcttccaca acaacgccac cgtgcacctg
     1081 gaccgggccc gcctggacgc ggtgctggcc gggctccccg aggccgtctt cgcggcgggg
     1141 ggaggggcgg ccaacacggt cagggccgcg gccctcctgg gcctcaaggc gagcttctcg
     1201 ggctcggtcg gcgaggacga gctgggcctg cgctaccgcg gcgagctcga ggaggccggc
     1261 gtcgagcccc tcctctccgc ctcccccgcg ccgacgggcg tctattgcgc cctggtgcgt
     1321 cccgacggcg ggaggagcct cctcgtcgcc ccctccgcgg ccctcgacct ggtcctggag
     1381 ccgccccctc aggccatgtt caaggcgggc gccatcctct acctggaagg cttcctcctc
     1441 cgcgaccggg ccttcttcat ggcctgcctg gaagggggcc gccgcgccgg gatgaagata
     1501 gcgctcgacc tgggaagctt ctccctggtc cgcgcccagc gcgacttcct cctcgagctc
     1561 ctgcccgact acgtcgacta cctcttcgcg aacgaggacg agttcagcgc cctggccgag
     1621 ctgcctttcg gcgaggccgt cgagctcctg gccgacgcgg ggccggccat cgtcgtgaag
     1681 ctggcggagc ggggcgcggt ctacgcgcgc gggggagagc ggatcgagag cccggtacgc
     1741 gccttgcggc ccgtggacga gaccggcgcc ggcgacgcct tcgccgccgg cttcctcgcg
     1801 gggatagccc gcggctacgc ccccgagcgc tgcctccgcc tgggcaaccg catcgccgag
     1861 gaggtcctgg gcgtgcccgg gctcgcggtg gattcggagc gcctcaaggc cgcctgggaa
     1921 tccgtctcgg cctaggagcg tccggcggga gcccctgggc cgatcttcca acgcggccga
     1981 ctgtccaaac ggcctggctc ggagccacat caaggcaagc ggccccaaat cgcggcagac
     2041 cgccccaaag caaggccaaa accttgccat ctccggctgg aagctttatt tttaagtcca
     2101 taaccccgag aaaggactta gccatgccgg aaaccgagat aatccccatc gagcagatac
     2161 tgccgaacaa gcttccgata atccccctca tgggacgccc catattcccc ggcatcttca
     2221 cgccgatcat ggtcggaaac cccgacgacg tgaaggtcgt ggaggaggcc ctggccgccg
     2281 acggcatgat cggcctcgtg atgatcaaga acgagttcga caagcccaag tccgacgacc
     2341 tccacgcggt gggcacggca gccaagatcg tgaagcggat caatcttccc gacggggggg
     2401 tcaacatctt catctccacg atgaagcgct tcaagatcaa gaagttcctc tccaaggaga
     2461 cgcccatcgt cgcggccgtg ggctatctgg acgaggagga cgacgactcc gacgaggtga
     2521 aggccctcac ccgcgcgctc ctgggcgaga tgaagcagat ctccgagaac aacccgctct
     2581 tctcggagga gatgcgcctc aacatgatca acatcgacca tccgggaaag atcgccgact
     2641 tcatcgcctc catcctcaac atcgacaaga acgaccagca gaagatcctg gagacgacca
     2701 acgtccggga ccgcatggag aaggtcctca tcttcatcaa gaaggagcag gagctcctca
     2761 agatccagaa gcgggtccag caggagatca acgagaagat cgagaagagc cagcgcgagt
     2821 acttcctcaa ggaggagctc aaggcgatca agggcgagct cggcatgccc acggacgcga
     2881 agggctccga ctaccagcgc ttcaaggaga agatcgagtc cttcggcttc gagggagagg
     2941 tgaaggagca ggtcgagcag gagctcgaga agttcaacct catggacgcg aactcgagcg
     3001 agttcatcgt cacccgcaac tggctcgacc tcatctgcaa cctgccctgg aaggcgccgg
     3061 tctccggcga cttcgacctc tcgcgcgcgc aggagatcct ggaggccgac cactacggcc
     3121 tcaaggacgt gaaggaccgc atcgtggagt acctcgcggt gcggaagctc aagaaggacg
     3181 cgaagggctc gatcctctgc ctcgtgggcc cgccgggcgt gggcaagacg agcgtcggcc
     3241 gctcgatcgc gcgcgccctc aacaagcagt tcttccgctt ctccgtcggc ggcatgcgcg
     3301 acgaggcgga gatcaagggc caccgccgca cctacgtcgg ggccctcccc ggcaagatcg
     3361 tccagggcct caagatcacg aagagccgcg accccgtctt catgatcgac gagatcgaca
     3421 agatgggctc ctccttccag ggcgacccct cgagcgccct cctcgagacc ctggaccccg
     3481 agcagaactt ctccttccgc gaccactacc tcgacctgcc cttcgacatg tcgaacgtct
     3541 tcttcatctg cacggccaac accctcgact ccatccccgg acccctcgtg gaccgcatgg
     3601 agatcatcca gctgccgggc tacatcgact ccgagaagct cgagatcgcg aagcactacc
     3661 tcgtcccgaa gtccctggag aagaacggca tcaggaagaa ccaggtgcgc tacacccgcg
     3721 acgcgctcat gttcatcgcc gagtcctacg cccgcgaggc gggggtgcgc aacttcgaga
     3781 aggccctgga caagatccac cgcaagctcg cgaaggagat cgtcctggag ggcaaggagg
     3841 gggacaagtt ccccgtcgac aaggccgccg tccagaagca gctcgggaag ccgctcttcc
     3901 gggaggacga catcaagaag gcgaccggcc ccggcatgtc gataggcctg gcctggacga
     3961 gcatgggcgg cgacaccctg gtcatcgagg cggtcgcgaa ccccggcaag gagggcttca
     4021 agctcacggg ccagatgggc agcgtgatgc aggaatcggc cggcatcgcc tacacccacg
     4081 tgcggcacat cgcctccgag cgctacggcg tcggggcgga ctggttcgag gggcggcagg
     4141 tccacctcca catcccggag ggcgccacgc ccaaggacgg cccctccgcc ggcatcacga
     4201 tggcgacggc cctcctttcc ctggtcctgg ggcggcgcat caaggaccgc ctcgcgatga
     4261 cgggcgagct ttccctcacg ggccaggtcc tgcccatcgg cggcctccgc gagaagaccg
     4321 tcgcggccaa gcggaacagg atccgcgaga tcgtcatccc gaaggcgaac gaaaaggacc
     4381 tggacgacat cccggagaac gtgaagaagg gcctgacctt ctacccggtc gagcgcttcg
     4441 aggaagtggt ggccatcgcc ttgccggact aggcaggcgg gtcaatcgag aggcgggagc
     4501 cccaaggctc ccgccgtttt ccctgggacc gcggccgtgg ccgcgcgccg cggcccctgg
     4561 agccgcctcg actaaaaacc caaaaatgtg ctatggttcc cggcccgaag gagtgcacgc
     4621 atgaacctgg ccctgaacaa gagcgtctcc ggcgaagtcc gcgagctcga gatccgcaag
     4681 aagaccccga tcctcagggc cttcgtgctc gccctcgtga tcctgcttcc gatcgtggcc
     4741 gcgagcgacc tcgtgacggg cgacaccctc ttcggcctta tcgaggtctt ccttttcgcg
     4801 gtcttcgtcg cctccttctt cctgctccgc gcgggaaagt acctcctcgt ctcacgcttc
     4861 gcctcgatcc tggtctggct cgccatggcc ttcatcagct acaacggccg ggcggacggg
     4921 acttcctcgg tctaccgcgt ggtcatcttc ctggcgacgg ccgtggcctt ctcgagcttc
     4981 ttcctcctgg acagcctcgt gccgatcgtc ctggcggcgg ccaacgccct cgtcatcggg
     5041 gcctactcct tcctccggct ggccggctcg atgtcggccg gggagacgct caacaacgtc
     5101 atcgtctcgc agctcttcaa cgccctcgtc ctcttcctcg tgatacagcc gacgcggctc
     5161 ggcaggtcga tatcggagga gctcgaggcg gagcgagggc ggggcgcgtc gcggctcgcg
     5221 acgctcaacc gggcggcggc gggcagcgag gagaacctcc gctccatcgg agccctggcg
     5281 ggccgcgtcg aggagatacg ctccgcggtg gagaccgcct ccaaggcggc agctaggatc
     5341 gagagcggcc ttgcccggat cgaccgcgcc gcggacgcgg ccacggcgga ggcggcctcc
     5401 atcggcgacc gcatgcgcga gctcaacggc cacatcgagg cggaggcctc cgcccaggtc
     5461 gagtccgccg cctcggtcaa cgagatggtc gcctccatcg gttccgtcgc ggattccgcg
     5521 cggaagcggc gggacggcat gcgggacctc gtcggcacca cggaggaggg acaggcgcgg
     5581 ctctcctccc tcctcgggat gatcggccgc gtcgagggca gcgtggacgc gatcaggcag
     5641 atggtcaccg tcatcaacaa gatcgcctcc tccacgaatc tcctcgccat gaacgcctcc
     5701 atcgaggccg cccacgccgg agaggccgga aagggcttct ccgtcgtggc cgaggagata
     5761 cgcggcctcg cggagaactc cgcgcggaac gcgaaggaga tcggccagcg gctcaaggac
     5821 gtcatccagg cgatcaccga ggcggccggc cagagcggcc tcatgaagga gtccttcggg
     5881 aagatccgcg aggagatcga cgccgccatc tcctccttcg acgagatcaa ggaggcgacc
     5941 ggcgagctct ccgagggcgg ccgccagatc ctcgagtcgc tcaaggtcct gaacgagacc
     6001 tccaccgggg tgcgtgaagg cggcgccttg atcctgggcg cccaggagcg gctcctcggg
     6061 ctgcagggcc aggccaagga cgcggtgagg gagctcgcgg gcgaggtggg cgtcgtcgcg
     6121 gaacggaacc aggcgcttct ctcctcggcc ctggccgtct ccgaggtcgc cgccgagggc
     6181 cagcgcaggg ccgaggagct ccacgccacg atgaacgggg cggacggagc ctccgcctag
     6241 gcgcctccgc ctaggcgcct ccgctaggaa ccgcccgaca gcctcacgaa ggcgttccgg
     6301 tcgttgccga gggcgagggc cgtatggacc agggccgcgg gcaggacgct gccgaagcgg
     6361 agcgtgaggg ccgaggccgc gacgccgaag accaggcagc cgagcgcctc cttgttgccc
     6421 ttgggcaagt gggcgaaggc gtagagcgcg gtgctcgccg cagccgccgc cagggcgccc
     6481 gccggcatga ggacggagag cagcattccc ctgaagacga actcgtaggc ggagagatag
     6541 aggagccagg agaggccctc ggccaggagc tcgcccgggc cccagcgcgc gggaaggtac
     6601 tgcgggtagt tcgccaggtc cgccttgtcc atcggaccca ggcggcccgc cgcatacgcg
     6661 acgagcgcga gcgcggccgc gggcatggcc caggcccgtg gatcgccggc gtagaggtac
     6721 tccaggccgg ggaggcgggc gcccaggagg gcgaagacgc ccaggggaag gatcgcga
//
LOCUS       DAOOSJ010000150         6764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6971_length_6764_cov_6.803995, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000150 DAOOSJ010000000
VERSION     DAOOSJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6971_length_6764_cov_6.803995"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLG14_08205"
     CDS             complement(<1..449)
                     /locus_tag="PLG14_08205"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOX49150.1"
                     /translation="MELSPLQKARYAYTPKLPPVLRGPAAGLAVERGPAALPAAEGDR
                     ERLQALFPASYGAGRVTLVRGKGEAAARAGAALKVGVILSGGPAPGGHNVIAGLFDGL
                     KAGNAASTLTGFRGGPGGLVDDKAVDITAELVGRYRNTGGFDIIGSGR"
     gene            complement(683..1837)
                     /locus_tag="PLG14_08210"
     CDS             complement(683..1837)
                     /locus_tag="PLG14_08210"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOX49151.1"
                     /translation="MNEIALTELKPKLYFTKYLYLDEGYVLLAPETPVTPELIARLTR
                     WEFRNLKTEGVPTELITGEEEGEAPAEEAPRPAENGPDDERLEGVLGFYAKFTEYVDG
                     LYTQFVTKNHLDYRELMERMKGVYELVAENKRLVLRALAAAPPLKNYLVSHAVRSSIL
                     SIALGSALKLPPFRLIELGGAAVLHEIGMVRLPAQLYMAGRQLSPVERKSITAHPVLG
                     YNLLKEHQVPLAVCLAALEHHERMNGSGYPRALTGDKISLYSRIIMVACSYDAVTSSR
                     PYKEAKEGYAGMVDILKNEGKQYDETVIRALVYSLSIYPIGSRVLLTSGKKALVVDVN
                     PENPRYPIVQILGAKTPDGKDIVIRTSETSVRVLRPLTKEEAAAEAQAKA"
     gene            1934..2809
                     /locus_tag="PLG14_08215"
     CDS             1934..2809
                     /locus_tag="PLG14_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49152.1"
                     /translation="MKFRTIYILFNVVIVVSFLFVFFLPFFVLGPSYSLSFWQGNWYL
                     GLFFVLLIGGLNAFFVLNWKLFVYVEREDWGALSAFLVDRIFTKRRYGNRHVALLVNA
                     YLLQSDVEGIERLEAELSSRRPELLRKNAVLFGVTRLLRNKPAEAEAFLARYLDAKDA
                     DDREWLRFDYAFSLVLQRRVAEALPQLRSGLASRDAVLALLSAYLVGSLGVSAASGQA
                     EKDELLRLAEASRASLRKRFPGQRWSKEVERAKNEVHIVILSKLIDDAGRWLFEAQAT
                     AAAAGSAQDQAGAPA"
     gene            2897..3058
                     /locus_tag="PLG14_08220"
     CDS             2897..3058
                     /locus_tag="PLG14_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49153.1"
                     /translation="MRPSRPNPRLAPAMAAVFVLLLPALLGAQPAAASPAAPASSPAL
                     LPFRAEGSS"
     gene            3059..>4329
                     /locus_tag="PLG14_08225"
     CDS             3059..>4329
                     /locus_tag="PLG14_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49154.1"
                     /translation="MATGSSLGATEAEALEAARGAALRALFAGLRKDRLFAEVFVRDP
                     PLGLAFETLSLERTGQGGWTAAVSLKVDDESLRIVARGPFLAAALSLLDGAEAEAAKA
                     DELLAAGAEAEARALLGEALGRYGQALDRLDAALALVEPLEDGSVLSTAKGRSSPELK
                     RALLASREGAQKGVARIRAAESALALDEADAAAAALVEAALEAVRRAESLLEEEGPVL
                     AEPSAYGPERLEPLRDRLLLERRALADAGAALDRALPAVPADKAYLRDKFDFAGRRLA
                     TLDASLLAAFRGVDRELRDPAARRAARARLWRWVFLHEPREHLALRLLPPPGLELEEG
                     EASLAEGSPFDFRLRSEDAFSFGSGGVWLRAGLESGEALLSVPEGQEGLERFLSSSFD
                     FGVWGRKGLWYAGYGWDWGRSLDGGASTPGQG"
     assembly_gap    4330..4429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4648..5550
                     /locus_tag="PLG14_08230"
     CDS             4648..5550
                     /locus_tag="PLG14_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX49155.1"
                     /translation="MRVSAAIAAFLAFGFLMAASLAAAEPEYHVLEKGETLYSLARKY
                     QLPYEAIARANGIADPGKLKVGQKILIPNLHRVEKGETLFGIAKAYGVSVDAIRAANK
                     LGASSVIKPGDVLYVPGGTKASGPAASASGAGTGAGASASAAGGAAAASGAAKSPAAE
                     PKPPVSSSVVLPPPVKTSAKPVDAKLSWPCAGEARYLDGKLFGVLIRAKAGEPSVAVA
                     GGSVVSAGPYRGFGQVAFVQSKAGYVYVYGGNESLSVGVGDRVKAGQELGRVGYDQKE
                     GRPVAYFFVFKEGEALDPARAPRE"
     gene            5795..>6764
                     /locus_tag="PLG14_08235"
     CDS             5795..>6764
                     /locus_tag="PLG14_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX49156.1"
                     /translation="MQPKKKAKTTSLALVKKKKAKHTKETDPLALYLKQISRYALLTA
                     EEELQIGEKIVKARARVRELDEARKGGLVPDDEWTEERERWEKELVGTKNRMISSNLR
                     LVVSIAKNYQHRGLSLLDLIDEGNIGLIEAVERFDYTRGCRFSTYGTWWIRQAIIKSL
                     ADKGRVIRIPIHMLNTIKKCYFVAKQLTQELGRDPNPEELADKLGMDSRKVKEIMKLS
                     QETASLDTTVDEDNVTHLSDLIKDENVAEPFEEVFSMALQDTLGDVLKNLSEREITII
                     QLRYGLNGEGPRTLEETGKLLGITRERVRQIQEKAIQKLKELQQLSE"
BASE COUNT          954 a         2406 c         2260 g         1044 t
ORIGIN      
        1 cggcccgagc cgatgatgtc gaagccgccc gtgttccggt agcggccgac gagctccgcc
       61 gtgatgtcga cggccttgtc gtcgacgagg ccgcccgggc ctccgcggaa gccggtcagg
      121 gtggaggcgg cgttgcccgc cttgaggccg tcgaagaggc ccgcgatgac gttgtggccg
      181 cccggcgccg ggccgccgga gaggatgacg ccgaccttga gggcggcgcc ggccctggcg
      241 gccgcctcgc ccttgccgcg gaccagggtc acgcggcccg cgccgtagga ggcggggaag
      301 agggcctgga ggcgctcgcg gtcgccctcc gcggcgggga gggcggcggg gccgcgctct
      361 acggcgaggc cggcggcggg gccgcgcagg acggggggca gcttgggggt ataggcgtag
      421 cgggctttct ggaggggaga cagctccatg ggtcactcct tcgggagagg tatcgtagtg
      481 gaataattct atatcggacg atagccctct gggaagacat cgcggcccgg ccgcccgcgc
      541 ttgcactgag gagccgcagc gcgcgacggg ggaaccgccg agcgcggcga gtgacgattg
      601 ggaggcgcgc ccgcgcgcgg cgagtgacgc ctggccggcg cgccatcgcg gggcgccctc
      661 ggcctcagcc ctgggccccg gcttaagcct tcgcctgcgc ctcggccgcc gcctcttcct
      721 tcgtgagggg ccttaggacg cggacgctcg tctccgaggt gcggatcacg atgtccttcc
      781 cgtcgggggt cttcgcgccc aggatctgga cgatggggta gcggggattc tccggattga
      841 cgtcgacgac cagggccttc ttgccgctcg tgaggaggac cctgctgccg atggggtaga
      901 tggagaggct gtagaccagg gcgcggatca cggtctcgtc gtactgcttg ccctcgttct
      961 tgaggatgtc gaccatgccc gcgtagccct ccttcgcctc cttgtagggg cggctggagg
     1021 tgacggcgtc gtaggagcag gcgaccatga tgatgcgcga gtagagggag atcttgtcgc
     1081 ccgtgagggc gcgggggtag cccgagccgt tcatgcgctc gtggtgctcc agggccgcca
     1141 ggcagacggc cagggggacc tggtgctcct tgaggaggtt gtagcccagg acggggtggg
     1201 ccgtgatgga cttccgctcc acgggggaga gctgcctgcc ggccatgtag agctgggcgg
     1261 ggaggcgcac catgccgatc tcgtggagga cggccgcgcc gccgagctcg atgaggcgga
     1321 agggcgggag cttcagggcc gagccgaggg cgatggagag gatggaggag cgcaccgcgt
     1381 gggagacgag gtagttcttg aggggaggag ccgcggcgag ggccctgagg acgaggcgct
     1441 tgttctccgc gacgagctcg tagacgccct tcatccgctc catgagctcg cggtagtcca
     1501 ggtggttctt cgtgacgaac tgggtgtaga ggccgtcgac gtactccgtg aacttggcgt
     1561 agaagcccag gaccccttcc agccgctcgt cgtcgggtcc gttctccgcg gggcggggag
     1621 cctcctcggc cggggcctcg ccctcctcct cgcccgtgat gagctcggtc gggacgccct
     1681 ccgtcttcag gttgcggaat tcccagcggg tcaggcgggc gatgagctcg ggcgtgaccg
     1741 gagtctcggg ggcgaggagg acgtagccct cgtcgaggta gaggtacttc gtgaagtaga
     1801 gcttcggttt aagctcagtc agcgcgatct cgttcatgca ccacccattg accccgccac
     1861 gcggcccgcc gtagactccc ggcaggccgt tcgccggaaa ctttcttctc tcttattata
     1921 ggagcgacgc cgcatgaaat ttaggacaat ctacatcctt ttcaacgtcg tcatcgtcgt
     1981 ttccttcctt tttgtcttct ttttgccctt tttcgtcctc ggcccctcct actccctctc
     2041 gttttggcag ggcaactggt atctgggcct cttcttcgtc ctcctcatcg gcgggctcaa
     2101 cgccttcttc gtcctcaact ggaagctctt cgtctacgtc gagcgcgagg actggggcgc
     2161 cctctcggcc ttcctcgtcg accgcatctt cacgaagcga cgctacggga accgccacgt
     2221 ggccctcctc gtcaacgcct acctcctcca gtccgacgtg gaggggatcg agcggctgga
     2281 ggccgagctt tcctccaggc gccccgagct cctgcgcaag aacgccgtgc tcttcggcgt
     2341 gacccgcctc ttgcggaaca agcccgcgga ggcggaggcc ttcctggcgc gctacctgga
     2401 cgcgaaggac gccgacgacc gcgagtggct ccgcttcgac tacgccttca gcctcgtcct
     2461 ccagcgccgc gtcgccgagg ccctgcccca gctccgctcg gggctcgcct cccgggacgc
     2521 cgtcctcgcc ctcctctccg cctacctggt cggcagcctc ggcgtctccg ccgcctccgg
     2581 ccaggccgag aaggacgagc tcctccgcct cgccgaggcc tcccgcgcct cgctcaggaa
     2641 gcgcttcccc ggccagcgct ggagcaagga ggtcgagcgg gccaagaacg aggtccacat
     2701 cgtcatcctc tccaagctca tcgatgacgc cggccgctgg ctcttcgagg ctcaggcgac
     2761 cgccgcggcc gccggatcgg cccaggacca ggccggagcc cccgcctgag agccgcctga
     2821 gagccgcctg agagccgccg cggcgccagg tcccgatttt taggcggctt gccgcctttc
     2881 cccgaaggag ccgtctatgc gcccatcgcg ccccaatccg cgcctggccc cggccatggc
     2941 ggccgttttc gtcctcctcc tcccggccct gcttggcgcc cagcccgcgg ccgcttcccc
     3001 agccgccccc gcttcctccc cggccctcct tcccttccgc gccgagggga gctcctaggt
     3061 ggcgacgggt tcgagtcttg gcgcgacgga ggcggaggcc ctggaggccg cgaggggcgc
     3121 cgccctgcgc gccctcttcg cggggctccg caaggaccgc ctcttcgccg aggtcttcgt
     3181 ccgcgacccg cccctgggcc tcgccttcga gaccctgagc ctggaacgga ccggccaggg
     3241 cggctggacc gcggccgtga gcctcaaggt ggacgacgag tccctgcgca tcgtcgcccg
     3301 cggtcccttc ctggccgcgg ccctgagtct cctggacggg gcggaggccg aggccgcgaa
     3361 ggcggacgag ctcctcgcgg ccggcgccga ggccgaggcc cgcgcccttc tgggcgaggc
     3421 cctgggccgc tacggccagg ccctggaccg tctggacgcg gcccttgccc tggtcgagcc
     3481 cctggaggac ggctccgtcc tctccacggc caagggacgg agctcccccg agctcaagcg
     3541 cgccctcctt gcttcccgcg agggagccca gaagggcgtc gcgcggatca gggccgccga
     3601 gtcggccctg gccctggacg aggcggacgc cgcggccgcg gccctggtgg aggcggccct
     3661 ggaggccgtc cgcagggcgg agagcctcct tgaggaggag ggccccgtcc tcgccgagcc
     3721 ctcggcctac gggcccgagc gcctcgagcc cctccgcgac cgcctcctcc tcgagcgccg
     3781 cgccctcgcc gacgcaggcg ccgccctgga ccgcgccctg cctgccgtcc ccgcggacaa
     3841 ggcctatctc cgggacaagt tcgacttcgc gggtcgccgc ctcgcgaccc tcgacgcctc
     3901 cctcctggcc gccttccggg gcgtggaccg cgagctccgc gatcccgccg cccgccgcgc
     3961 cgccagggcc cgcctctggc gctgggtctt cctccacgag ccccgcgagc acctggccct
     4021 gcgcctcctc cccccgcccg gcctcgagct tgaggagggc gaggcctccc tggcggaggg
     4081 ctcgcccttc gacttccgcc tccgctcgga ggacgccttc tccttcggct cgggcggggt
     4141 ctggctccgc gccggcctcg agagcgggga ggccctcctc tccgtaccgg aagggcagga
     4201 gggcctcgag cgcttcctct cgagctcctt cgacttcggc gtctggggcc ggaagggcct
     4261 ctggtacgcg ggctacggct gggactgggg ccggagcctg gacggcggcg cctccacgcc
     4321 cggccagggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcgccttcgc
     4441 cctccgcctg cccaagccct tcgcctgggg cctcgagtac tcgggctcgc gggccgcctc
     4501 cctgggcgaa tcgggctcct acgagggcga ggcgcggctc gagcagggcc tccgcttctt
     4561 cctcgaatac gcgctgtgag gcttcgcgcc ctgaaaaagc cggcggccct caagctcctc
     4621 cccctttcgc gccgatactc ggaacgaatg agagtctccg cggcgattgc ggccttcctg
     4681 gccttcggct tcctcatggc cgcctccctc gcggccgccg agcccgagta ccacgtgctc
     4741 gagaaggggg agaccctcta ttccctggcg aggaagtacc agctgcccta cgaggcgatc
     4801 gcgcgggcca acggcatcgc cgaccccggg aagctcaagg tcggccagaa gatcctcatc
     4861 cccaacctcc accgcgtgga gaagggggag accctcttcg gcatcgcgaa ggcctacggc
     4921 gtctccgtcg acgcgatccg cgcggccaac aagctcggcg cctcctccgt catcaagccc
     4981 ggcgatgtcc tctacgtgcc gggcgggacg aaggcctccg gcccggccgc ctccgcttcg
     5041 ggcgcgggca cgggcgcggg cgcgtcggcc tcggccgccg gcggggcggc cgccgcctcc
     5101 ggggcggcga agtccccggc cgcggagccc aagcccccgg tctcctcctc cgtcgtcctc
     5161 ccgcctccgg tgaagacctc cgccaagccc gtggacgcga agctcagctg gccctgcgcc
     5221 ggcgaggccc gctacctgga cggcaagctc ttcggcgtgc tgatcagggc caaggccggc
     5281 gagccctccg tcgccgtcgc cggcggctcc gtcgtctccg ccggccccta ccgcggtttc
     5341 ggccaggtgg ccttcgtgca gtcgaaggcc ggctacgtct acgtctacgg cggcaacgag
     5401 agcctctccg tcggcgtcgg cgaccgcgtg aaggcgggcc aggagctggg gcgcgtcggc
     5461 tacgaccaga aggaagggcg gccggtcgcc tacttcttcg tgttcaagga aggcgaggcc
     5521 ctggaccccg cgagggctcc ccgggaatga gcgccgcctt tcgcgcggag cgtacggttt
     5581 cggcgaaagg atacgggctc ggcggaaaac gcctcaaata tcagccgatt ctttccgatt
     5641 tcgcgatttt caaccgagaa agtatattat tgtctgtcgt cccggccggg cgtgatatat
     5701 tttttcccaa tcgggacgga tcaagaacgg aagcgccggg cgcagcacct ggaaggttgg
     5761 agacgctatg agcgaaagcg cttttcccaa gcctatgcag cctaagaaga aggccaagac
     5821 gacctccctc gcgctcgtca agaagaagaa agccaagcac accaaggaaa ccgatcccct
     5881 ggccctctac ctcaagcaga tctcgcgcta cgccctcctc acggcggagg aggagctcca
     5941 gatcggggag aagatcgtca aggcgcgggc ccgcgtccgc gagctcgacg aggcgcgcaa
     6001 gggcggcctc gtgcccgacg acgagtggac cgaggagcgc gagcgctggg agaaggagct
     6061 cgtcgggacc aagaaccgga tgatctcctc caacctgcgc ctggtcgtct ccatcgccaa
     6121 gaactaccag caccgaggcc tctccctcct cgacctcatc gacgagggca acatcggcct
     6181 catcgaggcg gtggagcgct tcgactacac ccgcggctgc cgcttctcca cctacggcac
     6241 ctggtggatc aggcaggcca tcatcaagag cctcgcggac aagggccgcg tcatccgcat
     6301 cccgatccac atgctcaaca cgatcaagaa gtgctacttc gtcgccaagc agctcaccca
     6361 ggagctgggc cgcgacccca atcccgagga gctcgcggac aagctgggca tggactcgcg
     6421 gaaggtgaag gagatcatga agctctccca ggagaccgcg agcctggaca cgacggtgga
     6481 cgaggacaac gtcacccacc tctccgacct catcaaggac gagaacgtcg ccgagccctt
     6541 cgaggaggtc ttctccatgg ccctccagga caccctgggc gacgtcctca agaacctctc
     6601 cgagcgggag atcacgatca tccagctccg ctacggcctc aacggcgagg gcccccgcac
     6661 cctggaggag accgggaagc tcctcgggat cacgcgcgag cgcgtccgcc agatccagga
     6721 gaaggccatc cagaagctca aggagctgca gcagctctcc gagt
//
LOCUS       DAOOSJ010000151         6754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_6988_length_6754_cov_5.759218, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000151 DAOOSJ010000000
VERSION     DAOOSJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6988_length_6754_cov_5.759218"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..>1479)
                     /locus_tag="PLG14_08240"
     CDS             complement(144..>1479)
                     /locus_tag="PLG14_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49157.1"
                     /translation="GLRKRLLSMFSLYGGLPKPVYALFAATVINGVGIFVFPFLTLFL
                     TKRLGWSASRSGDFMLLSSLAYIPGTLIGGRLADRFGRKKVMLASQALAGLMFVPCGF
                     LGDSGLVPWFILASLFFDGVTDPARSAMQTDLTTPENRQAAFSLIYLGHNLGFAFGPL
                     IAGFLFYGATPWLFWGNALAILAALALVARLVPETKPTEEAIRATIGSGSTEEAHEGG
                     LLSALASRPYLLVYTLLTTFYGFVYAQHRFALPLQTDAWFGQSGTRLYGSLMTLNAVM
                     VVALATPVVALTRRWKPVSNVSAAGFLFAAGFAMVAFARTPLLVFVSTAVWTLGEIVN
                     ATSDGAYVANHTPMSHRGRFQSILPIIGGTGFAVSSSVVGGIIERSGLAPVWPLLGLV
                     AAAAATGIALLGWVEARRAGRTSAVLPSGAEAPEAVAEAAEAAQAAPGAPAD"
     assembly_gap    1480..1579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1580..2341
                     /locus_tag="PLG14_08245"
     CDS             <1580..2341
                     /locus_tag="PLG14_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOX49158.1"
                     /translation="GTKPSIAYIVEEFVASGITEIAIISSRRKKALEDYFDREIELEE
                     VFRREGKADKLALVAPPEADVVFVRQREMRGTGHALLQVRSFVGSEPCVVAYPDDLHV
                     GAKPLAAQLVEVHARTGKSVMATILEPGDVSRYGVVDPAPDGEGVRGFVEKPARGAEP
                     SHEVSIGRYLYTPEFFDLLAEGWARHGKGEYFHTYALDRLIAAGKVAYRRVEGERLDT
                     GEPAGYLDAVLRNAAADPSLRPVLERFARETLGLK"
     gene            2354..3910
                     /locus_tag="PLG14_08250"
     CDS             2354..3910
                     /locus_tag="PLG14_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49159.1"
                     /translation="MQDPQAILAKFVPARLGADLAKLSPGVRAALPHLKRALDGLHRV
                     YLGQLDEGLPARYEDVMAGPPSAKRDFYEFFLGPWNPLEGDALVFPGEPEKRPGAALY
                     PAGLSAAEFSRRAAAAEPALRESLMDHYSVVEEKDGALRATAYHEKYAELLAPIAAAL
                     RAAAAEARADGAAAAPLCGYLEERASSLLSGGYREADATWVRLRDTPLELVLGPYEVY
                     LDGVAGVKAAYEGMLFAVDAEKGRSLRDIEKGLGELADAFPLPSGSRSAVGGLAPIVV
                     VDLLYSSGEARQGVMAAAFNLPNDPWVRGNVGWKQVMIRNVMEAKFSKIGAPISRAIL
                     GDSAAGFEPFFYFVLLHEVSHGLGPAFRADGTAVDAALGARYTAIEEAKADTGALHLM
                     LAKGGRAGVPAFGERELLDSYVAGLFRSMRFGLHEAHGAANLIEFNWLAEKGVLSWGA
                     DGRLASRPANLRSAAASLLEELCRLEAAASPAEAEAFIAKYAAVSPELEAAIAGLSSI
                     PTDIRPVFEL"
     gene            complement(4031..6541)
                     /locus_tag="PLG14_08255"
     CDS             complement(4031..6541)
                     /locus_tag="PLG14_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOX49160.1"
                     /translation="MCGIVSIAYGDDNPDIGKEGAALLRRLEYRGYDSTGAAFIDGKG
                     LVSLMKKVGSPTRVCRELGVERWGGRRFIGQVRWATYGAVTDVNSQPHRVVCKVELVG
                     AHNGNVSNTDSLKPWLAERGHKVVSDNDGEIVVHLVEEAYAAGLAPGGGFSPGGQAAL
                     AAARLALQAAGQEAAASNPADGALLLVDAARKADALAEGSYAAAVADPRGAGVVAMKS
                     GSSLYAGLGRDGRGDFVVVSSDLSSVLSKTRSLIPLSEGEGLWFTERDYLVFPLSGEL
                     RFSRPKPKRSKLDVRDTALDPKYRHFMEQEIAAEPENLDRILRYYFADEASAPLAAAL
                     EAVRPAAEEAADALLALADLPAGAAASAGLAALLGSAAFREAASRAAGAALPPETAAA
                     ASGGPFSSDERALMEELAAGTPGARTDLALLDSVLSWRKRRETAGRYRDLLDALRETE
                     RRGGRTFLVASGTSYHAALVAASFFDELARVPVYPCNPGQFRSMYLGSLKESDLLVAI
                     SQSGETKDLVDALQDAAERVPRLRRASIVNNENSRIPQELSDFYLPILCGPETAVAAT
                     KSFTNQLAVLYALAMGLGHGEGEALRRLRKARALVAESFASTAAAVDAVAERLFQRPS
                     IHMLGSSLVGLAREGALKVREVVLCHAEGYDAAEFKHGPNTILGKNEIFSAEDVAKAI
                     GLYREAVAAEPALAAKPPLEVMRARPEILEGLFRNYPLVFVCPPSERDVKITVSQIHT
                     HKIRGADVVVIAEPSKELAAAASGRPAGDEAYWSRVIELPASGDPALFVFAAAAVLQR
                     LAYRMSVLKSEWLDALGVQAHGVHPDVPKNVSKSITVD"
BASE COUNT          955 a         2260 c         2545 g          894 t
ORIGIN      
        1 cgcgcgctgt cgtcgaggcg gcgccggcgg ccggattgcc tgcgtcgcgc gctggcgtcg
       61 cggcggcgcc ggcggccgcg tccttcgggc tgccggcgca gcgagctccg cggcgattta
      121 cggcggcgcg cgccgcggcc gtcctagtcc gccggggcgc cgggcgcggc ttgggccgcc
      181 tccgccgcct cggccacggc ttccggggcc tccgcgccgg aaggcagaac ggcggacgtc
      241 cgcccggcac gccgcgcctc cacccacccg agtagcgcga tgcccgtagc cgcggccgcg
      301 gcgacgaggc cgaggagcgg ccagacgggc gcgaggccgc tgcgctcgat gatgccaccg
      361 acgacggagg agctcacggc gaagcccgtc ccgccgatga tcggcaggat ggactggaag
      421 cggccccggt ggctcatggg cgtgtggttc gccacgtagg cgccgtcgct cgtcgcgttg
      481 acgatctcgc ccagggtcca gaccgcggtg gagacgaaga cgaggagcgg cgtccgcgcg
      541 aaggcgacca tcgcgaagcc ggcggcgaag aggaagcccg ccgccgagac gttcgagacc
      601 ggcttccagc gcctggtcag ggcgacgacg ggcgtggcca gggcgacgac catgaccgcg
      661 ttgagcgtca tgaggctgcc gtagaggcgc gtgccggact ggccgaacca ggcgtcggtc
      721 tgcaggggca gggcgaagcg gtgctgggcg tacacgaagc cgtagaaggt cgtgaggagg
      781 gtgtagacga ggaggtaggg ccgcgaggcc agggccgaga ggaggccgcc ctcgtgggcc
      841 tcctcggtgg agccggagcc gatcgtcgcg cggatcgcct cctccgtcgg cttggtctcg
      901 ggcacgaggc gcgccacgag ggccagggcc gcgaggatgg cgagggcgtt gccccagaag
      961 agccagggcg tcgcgccgta gaagaggaag cccgcgatga gggggccgaa ggcgaagccg
     1021 aggttgtggc cgaggtagat gaggctgaag gctgcctgcc ggttctccgg ggtcgtgagg
     1081 tcggtctgca tcgcgctcct cgccggatcc gtgaccccgt cgaagaagag gctcgcgagg
     1141 atgaaccagg gcacgaggcc cgagtccccc aggaagccgc agggcacgaa catgaggccc
     1201 gcgagggcct gggaggcgag catcaccttc ttcctgccga acctgtccgc gagccggccc
     1261 ccgatcagcg tgccggggat gtaggcgagg gaggagagga gcatgaagtc gccgctcctg
     1321 gacgcggacc agccgaggcg cttcgtgagg aagagggtga ggaaggggaa gacgaagatg
     1381 cccacgccgt tgatcacggt ggccgcgaag agggcgtaga cgggcttggg caggcctccg
     1441 tagagggaga acatggagag gagcctcttc ctgaggccgn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnng ggacgaagcc ctcgatcgcg tacatcgtcg aggagttcgt
     1621 cgcctccggc atcacggaga tcgcgatcat cagctcgcgc cgcaagaagg cgctcgagga
     1681 ctacttcgac cgcgagatcg agctcgagga ggtcttccgc cgcgagggga aggccgacaa
     1741 gctcgccctg gtcgcgccgc ccgaggccga cgtggtcttc gtccgccagc gcgagatgcg
     1801 cggcaccggc cacgccctcc tccaggtccg ctccttcgtc gggtcggagc cctgcgtcgt
     1861 cgcctacccg gacgacctgc acgtcggcgc gaagcccctg gcggcccagc tcgtcgaggt
     1921 ccacgcgcgc acgggcaagt ccgtgatggc cacgatcctg gagccgggcg acgtctcccg
     1981 ctacggcgtc gtggatcccg cccccgacgg cgagggcgtc cgcggcttcg tggagaagcc
     2041 ggcgcgcggg gcggagccct cccacgaggt ctccatcggg cgctacctct acacgcccga
     2101 gttcttcgac ctcctcgcgg agggctgggc ccggcacggg aagggcgagt acttccacac
     2161 ctacgcgctg gaccgcctca tcgccgccgg caaggtggcg taccggcgcg tcgagggaga
     2221 gcgtctggac acgggcgagc ccgcgggcta cctggacgcg gtcctcagga acgcggccgc
     2281 cgatccctcc ctccgccccg tcctggagcg cttcgcccgc gagacgctcg gcctcaagta
     2341 aaggagaaca gccatgcagg atccccaagc catactcgcc aagttcgtgc ccgcgcgcct
     2401 tggcgccgac ctcgcgaagc tctccccggg cgtccgcgcc gccttgcccc acttgaagcg
     2461 cgccctggac ggcctccacc gcgtctacct cggccagctc gacgaagggc tccccgcgcg
     2521 ctacgaggac gtgatggccg gcccgccctc cgcgaagcgc gacttctacg agttcttcct
     2581 gggcccctgg aaccccctgg agggcgacgc cctcgtattc ccgggcgagc ccgagaagcg
     2641 gcccggggcg gccctctacc ccgccggcct cagcgccgcc gagttctccc gccgcgccgc
     2701 ggccgcggag cccgccctgc gggagagcct catggaccac tacagcgtcg tcgaggagaa
     2761 agatggcgcg ttgcgcgcga cagcctacca cgagaagtac gcggagctcc tcgcgccgat
     2821 cgcggccgcg ctcagggcgg ccgccgcgga agcccgcgcc gacggcgccg cggccgcgcc
     2881 gctgtgcggc tacctggagg agagggcctc gagcctcctc tccggcggct accgcgaggc
     2941 ggacgcgacc tgggtgaggc tccgcgatac gcccctggag ctcgtcctcg ggccctacga
     3001 ggtctacctg gacggcgtcg cgggcgtgaa ggccgcctac gagggcatgc ttttcgccgt
     3061 ggacgcggag aagggccgct ccctcaggga catcgagaag ggcctaggcg agctagccga
     3121 cgccttcccc ctgccctcag gctcgcgctc cgcggtgggc ggcctcgcgc ccatcgtcgt
     3181 cgtcgacctc ctctactcct cgggcgaggc gaggcagggc gtgatggccg cggccttcaa
     3241 cctgccgaac gacccctggg tccgcggcaa cgtcggctgg aagcaggtga tgatccgcaa
     3301 cgtgatggag gccaagttct cgaagatcgg cgcgcctatc tcccgggcga tcctgggcga
     3361 ctcggccgcg ggcttcgagc ccttcttcta cttcgtcctc ctccacgagg tgagccacgg
     3421 cctggggccc gccttccgcg cggacgggac ggcggtcgac gcggccctgg gcgcgcgcta
     3481 taccgcgatc gaggaggcga aggccgacac gggggccctg cacctcatgc tcgcgaaggg
     3541 cggccgcgcc ggcgtgcccg ccttcggcga gcgcgagctc ctggacagct acgtcgcggg
     3601 gctcttccgt tcgatgcgct tcggcctcca cgaggcacac ggcgcagcca acctcatcga
     3661 gttcaattgg ctcgccgaga agggcgtcct gtcctggggc gcagacggcc gcctcgcttc
     3721 gaggccggcc aacctgcgct cagccgcggc ctccctcctc gaggagctct gccgcctgga
     3781 ggcggccgcc agccccgcgg aggccgaggc cttcatcgcc aagtacgccg ccgtctctcc
     3841 cgagctcgag gccgccatcg cgggcctctc ctcgatcccg acggacatta ggccggtctt
     3901 cgagctgtag gctcgctccg gcgcggcttc gcgccgcgga caagagaaag ccggcccctc
     3961 ggggccggct ttcgttattc tttgcgccgg ggcgcttcgc cgatcgccgt gggcaccccg
     4021 acgtcccgcc tcaatccacg gtgatgctct tgctcacgtt cttcgggacg tcggggtgga
     4081 cgccgtgggc ctgtacgccc agggcgtcga gccactcgga cttgaggacg gacatgcggt
     4141 aggcgaggcg ctggaggacg gccgccgcgg cgaagacgaa gagggcgggg tcgcccgagg
     4201 cggggagctc gatcaccctg gaccagtagg cctcgtcgcc cgcggggcgg ccggaggcgg
     4261 ccgcggcgag ctccttggag ggctcggcga tgacgacgac gtcggcgccg cggatcttgt
     4321 gcgtgtggat ctggctcacg gtgatcttca cgtcgcgctc cgagggcggg cagacgaaga
     4381 ccagcgggta gttcctgaag aggccctcca ggatctccgg cctcgcgcgc atgacctcca
     4441 ggggcggctt ggcggcgagg gcgggctcgg cggcgacggc ctcgcggtag aggccgatgg
     4501 ccttggcgac gtcctccgcc gagaagatct cgttcttccc gaggatcgtg ttggggccgt
     4561 gcttgaactc ggcggcgtcg tagccctccg cgtggcagag gacgacctcc cggaccttga
     4621 gggcgccctc gcgggccagg cccacgaggc tcgagccgag catgtggatg gaggggcgct
     4681 ggaagaggcg ctccgcgacc gcgtccacgg cggcggccgt ggaggcgaag gactccgcga
     4741 cgagggcccg ggccttcctc agccgccgga gggcctcgcc ctccccgtgg cccaggccca
     4801 tggccagggc gtagaggacg gcgagctggt tcgtgaagga cttcgtcgcc gcgacggcgg
     4861 tctccggccc gcagaggatc gggaggtaga agtccgagag ctcctggggg atgcggctgt
     4921 tctcgttgtt gacgatggag gcgcggcgga ggcggggaac gcgctccgcg gcgtcctgga
     4981 gggcgtcgac caggtccttg gtctcgccgg actgcgagat cgcgacgagg aggtcgctct
     5041 ccttgaggct cccgaggtac atcgagcgga actggccggg gttgcaggga tagacgggga
     5101 cgcgcgcgag ctcgtcgaag aaggaggctg cgaccagggc ggcgtggtag gaggtgcccg
     5161 aggcgacgag aaaggtccgg ccgccgcggc gttcagtttc gcgcaacgcg tcaaggaggt
     5221 cgcggtagcg gccggccgtc tcgcggcgct tccgccagct gaggactgag tcgaggaggg
     5281 cgaggtcggt cctcgcgccg ggagtccccg ccgcgagctc ctccatgagg gcgcgctcgt
     5341 cggaggagaa ggggccgccc gaggcggcgg cggccgtctc cggcgggagg gcggcgccgg
     5401 cggcgcggga ggcggcctcg cggaaggccg ccgagccgag gagggctgcg aggccagccg
     5461 aggcggccgc gcccgcgggg aggtcggcca gggcgaggag ggcgtcggcc gcctcctccg
     5521 ccgcggggcg gaccgcttcc agggccgccg cgaggggggc cgaggcctcg tccgcgaagt
     5581 agtagcgcaa gatccggtcc aggttctccg gctccgccgc gatctcctgc tccatgaagt
     5641 ggcggtactt cgggtcgagg gcggtgtcgc gcacgtcgag cttggagcgc ttgggcttgg
     5701 gccgggagaa gcggagctcg ccggagagcg ggaagacgag gtagtcgcgc tccgtgaacc
     5761 agagcccctc gccctcggag agggggatga gggagcgggt cttggagagg acggaggaga
     5821 ggtcggagga gacgacgacg aagtcgccgc gcccgtcgcg gcccaggccc gcgtagaggg
     5881 aggagccgga cttcatcgcg acgacgccgg ctccgcgggg gtcggcgacg gcggccgcgt
     5941 aggagccctc agcgagggcg tcggccttgc gcgcggcgtc gacgaggagg agggccccgt
     6001 cggccgggtt cgaggccgct gcttcttgtc cggcggcctg gagggcgagc ctggccgcgg
     6061 cgagggcggc ctgtcctccg ggcgagaagc ctccgcccgg ggcgaggccc gcggcgtagg
     6121 cctcctccac gaggtggacg acgatctcgc cgtcgttgtc ggagacgacc ttgtggccgc
     6181 gctcggccag ccagggcttc agggagtcgg tgttggagac gttgccgttg tgcgcgccca
     6241 cgagctcgac cttgcagacg acccggtggg gctggctgtt gacgtcggtg acggcgccgt
     6301 aggtggccca gcgcacctgg ccgatgaagc ggcggccgcc ccagcgctcg acgccgagct
     6361 cgcggcagac gcgggtcggc gagccgacct tcttcatgag ggagacgagg cccttgccgt
     6421 cgatgaaggc cgctcccgtg gagtcgtagc cgcggtactc gagcctgcgg aggagggcgg
     6481 cgccctcctt gccgatatcg ggattgtcat cgccgtaggc gatcgatacg attccgcaca
     6541 tagtaacgct ccttttaata gttgaaacgg gacgtctcca gtatacacga tgagccgcat
     6601 cctgatcgag aaccgccgcc gcgagccgtc gagcgtcgcg atcaggccgc ggatggagag
     6661 cgggggtgcc ggaggcgacc ggagcccgcg ccgcgacaga tcagttgtgc atatgcacaa
     6721 ctgacttcga tcccgccgat cggcgcgggc gagg
//
LOCUS       DAOOSJ010000152         6718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7022_length_6718_cov_4.876932, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000152 DAOOSJ010000000
VERSION     DAOOSJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_6718_cov_4.876932"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLG14_08260"
     CDS             complement(<1..1401)
                     /locus_tag="PLG14_08260"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOX49161.1"
                     /translation="MAERTAMMDQYRRAKSRHRDAVLFFRLGDFYEMFFEDAVEVSAL
                     LNLTLTKRQGEPMCGVPYHAARSYIARLLKLGKKIAVCEQLSEPGQGKGLVERDVVEV
                     ITPGTAMEEDFLDQGANNYLLSLCEAKGRLACAYVDISTAEFRAFALKEGGGAAVEER
                     LRKELYRLAPREVLVQQSLLERPEVSGPLAERGSMIVNRLPDWSFDAAAGGEDIRSRF
                     GLATLKGLGFDEEDPELAAAAALLRYVDDTAASAAPHLSSLRPYVEEDFALVDESSQK
                     NLELVQNLHDGGRAYSLLEVLDHTKTAPGSRLLRQWLLQPLRRKEAVEARLDAVEFLY
                     RDQLLLQSLREALGRLLDAERLASRVAMDKAHAKDLLALGDSIAAAEAARVLLEKAGA
                     RGLLAAGPGAEERAAALALRELVGRAVKEEPSTLLAEGDLIREGYDPELDRLRSLKDG
                     ARAVLEAANGPLRTGSV"
     gene            1467..1958
                     /locus_tag="PLG14_08265"
     CDS             1467..1958
                     /locus_tag="PLG14_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX49162.1"
                     /translation="MEEAVFIAEAEGRIYVRAQGHVTAALCPELKTRIFPRLEAEPGV
                     EAVYFDLGPCEYMDSTFLGLIVGINKRLKLAASRPVVLLHVNDTCLGLLKTIGVTKLV
                     ELSTEEVPFPHGLSRVGPGPRATAEFLLDAHEELSDLSEENKKRFSTLTDTLRSALGK
                     GED"
     gene            complement(2173..2724)
                     /locus_tag="PLG14_08270"
     CDS             complement(2173..2724)
                     /locus_tag="PLG14_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOX49163.1"
                     /translation="MKRTTLAAAALALVLAFGAAGAAGAQQITRIAVVDLQKIIMTYS
                     KDGTALRDFEAKKAQIQAEIDKMGEELKKLQAQKVEAEKSGDKQGALRIESDLYKKTE
                     FLKEYARAKQAELDDQAKKLGSSNQFVQLVYRQIQLVAETEGFSLVLNLKSADSVMSA
                     VVWYSPMIDITDKVIQAMIGKAQ"
     gene            complement(2741..5263)
                     /gene="bamA"
                     /locus_tag="PLG14_08275"
     CDS             complement(2741..5263)
                     /gene="bamA"
                     /locus_tag="PLG14_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOX49164.1"
                     /translation="MKRLCLFIALALSLAAPAAFGEEAAADWFWGKPIAGVQWEGVKN
                     ADKRELDSAMRPYVGKAFSEELWVELQARVYELDWFDPATIQSAAFPADEEKTKVLIK
                     FTVTEKPAVDAVRVVGNSGLRSSDILDVVKIKAGDIFNPSKTKLDELAVRRLYLEKGY
                     PDAQVSSSTSTGPSGGVVLSFAVVEGSQVAIKEIRFTGNVAFSSQTLKGQLSLKEAGL
                     FQAGAFQELKLEEDKQKVVAYYRARGYVDAAVADVVRSVQKDEKSGKNWLVLTFALKE
                     GRQWTYAGMAFEGNEIFGSDKLAALVFQKEGGILNYGKLMQDKQRIDDLYYENGYIFN
                     SLDLAERRDEEKLSIAYTVRIVERDRAHIESITFKGNKKTKEAVMARELPLEVGDIFS
                     KAKIVEGLRNLYNLQYFSAIEPEMLPGGGENLMDLVINVEEQSTADIQFGVTLSGLGE
                     PDTFPVSGLIKWNDKNFLGNGQTFGIETNISPDTQDLTFSFLDNWLFGKRISGGIDLS
                     LKHEQLWVGQDILGPIFYDEDVPDPYDSLAEFEAAGSSISSAYKMPYENWEVTLGLSG
                     GYKIPTQIGDVGFGLGLSSTLSNVSYDSSKYRPYLKELRDAEGLWLLTNKVLVRGYLN
                     DLDLWYNPGKGYYASQRLTWAGVFAGEAQKYIRSDSRLDAFATLFNIPVFEGWNFKWV
                     LGAHTGFSAILPQPGASASVIKTDALRIDGTFVGRGWSSLYDVEEGRTLWENWVELRM
                     PIFEQFIWLDGFVDAAALRTETGLVVPTDGAANAAQRDFSSLAWKNFAFSTGFGFRFT
                     IPQFPFRFYFAKRFAFDGKSIDWAPDGASGLDFVISISQPLK"
     gene            complement(5281..>6718)
                     /locus_tag="PLG14_08280"
     CDS             complement(5281..>6718)
                     /locus_tag="PLG14_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOX49165.1"
                     /translation="GKLEAEVTGLGVDLSPFLGLLGPLPVGLEGGRLEGSLSVGGSLA
                     DPEFSGSLNLDGIAVTVPDIIAGKIGPMTLPVLVDGKRLSLSVPSLPIGTARASLRAE
                     ALLDGWRIGEISASLRNLPGAPLPLDVKILGVLVAGSAYADLDFSYSGDVARLGGKVS
                     VERSRVVINPGLFKAEGSRRKPQTFFDLDLDIGFGTGVQVLFPSEDLPIVSGYAEPSS
                     RLALKLFGEAQEVSLKGTAFLRGGDVFYIQRNFFLKSAKIVFNETSGHFDPLVTLYAE
                     RRERNDRGPILVMLRAENAPASNFKPQLSSSPSLSEEEIAALLGEGLLGVSESGEVDI
                     RKAVIASSEFIPQLNATKAFESRVRQALGLDIFSLRSQVVQRWLLDISSLDRGTEPTG
                     LGSYLDETSLFAGKYLDERTFLYASLSLREEDPLVPSGPLLLDSEVGVEFDTPFGTLL
                     WSVTPTSKSLENLYISDQSLSLSWRLPL"
BASE COUNT         1051 a         2126 c         2416 g         1125 t
ORIGIN      
        1 caccgaaccg gtacgcaagg gaccgttcgc cgcctccagc acggccctgg cgccgtcctt
       61 gagggacctg agcctgtcga gctcggggtc gtagccctcg cggatgaggt cgccctcggc
      121 gaggagggtg gagggctcct ccttcacggc gcgcccgacg agctcgcgga gggccagggc
      181 cgcggccctc tcctcggccc cgggaccggc tgcgaggagt ccccgggccc cggccttctc
      241 gaggaggacg cgggccgcct ccgccgcggc tatggagtcg cccagggcca ggaggtcctt
      301 cgcgtgggcc ttgtccatcg cgacgcgcga agccaggcgc tccgcgtcca ggaggcggcc
      361 cagggcctcg cggagagact ggaggaggag ctggtcgcgg taaaggaatt ccacggcgtc
      421 gagcctggcc tccacggcct ccttgcggcg caggggctgg aggagccact ggcggaggag
      481 cctggagccg ggggccgtct tcgtgtggtc caggacctcg aggaggctgt aggcgcggcc
      541 gccgtcgtgg aggttctgca cgagctccag gttcttctgg ctcgactcgt ccacgagggc
      601 gaagtcctcc tccacgtagg ggcggaggct cgagaggtgg ggcgcggccg acgccgccgt
      661 gtcgtccacg tagcggagga gggccgcggc ggccgccagt tcgggatcct cctcgtcgaa
      721 gccgaggccc ttgagcgtcg cgaggccgaa gcgggaacgg atgtcctcgc cacccgccgc
      781 cgcgtcgaag gaccagtcgg ggagccggtt cacgatcatg gagccgcgct cggccagggg
      841 ccccgagacc tcgggccgct caaggaggct ctgctggacg aggacctcgc ggggcgcgag
      901 gcggtagagc tccttccgga gcctctcctc gacagccgcg ccccccccct ccttgagggc
      961 gaaggcgcgg aactcggccg tggagatgtc gacgtaggcg caggccaggc ggcccttcgc
     1021 ctcgcagagg gacaggaggt agttgttggc cccctggtcc aggaagtcct cttccatcgc
     1081 ggtgccgggg gtgatcacct ccacgacgtc gcgctcgacg aggcccttgc cctggcccgg
     1141 ctcggagagc tgctcgcaga cggcgatctt cttcccgagc ttgaggaggc gggcgatgta
     1201 ggaacgggcg gcgtggtagg gcacgccgca catgggctcg ccctggcgct tggtcagggt
     1261 gaggttgagg agggcgctca cctcgacggc gtcctcgaag aacatctcgt agaagtcgcc
     1321 gagcctgaag aagagcacgg cgtcccggtg gcgggacttg gccctccggt attggtccat
     1381 catcgcggtt cgctcggcca tggccgttgc attatagcgg ccgccgcggt aatctacaag
     1441 gcgaggcatt tccaggagga cgcaaggtgg aagaggcggt attcatcgcg gaggcggagg
     1501 gcaggatcta cgtcagggcc caggggcacg tgaccgccgc cctctgcccc gagctcaaga
     1561 cgaggatctt ccccaggctc gaggccgagc ccggcgtcga ggccgtctat ttcgacctcg
     1621 gaccctgcga gtacatggac tcgaccttcc tcggcctcat cgtcggcatc aacaagcggc
     1681 tcaagctcgc cgcctcgagg ccagtcgtcc tcctccatgt caacgacacc tgcctgggcc
     1741 tcctcaagac catcggcgtc acgaagctcg tggagctctc cacggaggag gttcccttcc
     1801 cccacggcct ctcgcgcgtc ggccccgggc cccgcgccac ggcggaattc cttttggacg
     1861 cgcacgagga gctctcggat ctttccgagg agaacaagaa gcgcttctcc accctcaccg
     1921 acaccctgcg gagcgccctg ggcaagggag aggactgaag gccgggcaaa ggcgcgaagc
     1981 cgaaggcggg aagccgcgcc ggtcaccgga cagcccggcc gtcggccgga tcgcgccgcg
     2041 gagagcggga cgagggaagg ccgccccgaa gggaactcgc tcccctcgag gcggccttcc
     2101 tcatgtcggc cttgttccat ggcggcggct cgggccgcgc gggaggccgg gccgctggcc
     2161 gaaggcgcca ggctactggg ccttcccaat catcgcctgg atgaccttgt cggtgatgtc
     2221 gatcatcggg ctgtaccaga cgacggcgct catcacggag tcggcgctct tcaggttgag
     2281 gacgagggag aagccctcgg tctcggcgac gagctggatc tggcggtaga cgagctggac
     2341 gaactggttc gaggagccga gcttcttcgc ctggtcgtcg agctcggcct gcttggcgcg
     2401 ggcgtattcc ttgaggaatt ccgtcttctt gtacaggtcg gactcgatcc tcaaggcgcc
     2461 ctgcttgtcg ccggacttct cggcctccac cttctgggcc tggagcttct tcagctcctc
     2521 gcccatcttg tcgatctccg cctggatctg cgccttcttg gcctcgaagt cgcggagggc
     2581 ggtcccgtcc ttcgagtagg tcatgatgat cttctgcagg tcgacgacgg cgatccgcgt
     2641 gatctgctgc gcgcccgcgg cgccggcggc gccgaaggct aggacgaggg ccagtgcggc
     2701 cgcggccagg gttgtgcgct tcatctctat cgtcctccct ttacttgagc ggctggctga
     2761 tgctgatgac gaagtccagg cccgaggcgc cgtcgggcgc ccagtcgatc gacttcccgt
     2821 cgaaggcgaa gcgcttcgcg aagtagaagc ggaaggggaa ctgcgggatc gtgaagcgga
     2881 agccgaagcc cgtggagaag gcgaagttct tccacgcgag ggaggagaag tccctctggg
     2941 ccgcgttggc ggctccgtcc gtcgggacga cgaggccggt ctcggtgcgc agggcggccg
     3001 cgtccacgaa gccgtcgagc cagatgaact gctcgaagat gggcatgcgg agctcgaccc
     3061 agttctccca gagcgtcctg ccctcctcga cgtcgtagag ggagctccag ccgcggccga
     3121 cgaaggtgcc gtcgatgcgc agggcgtcgg tcttgatcac ggaggccgag gcgccgggct
     3181 ggggcaggat ggcggagaag ccggtgtgcg cccccaggac ccacttgaag ttccagccct
     3241 cgaagacggg gatgttgaag agggtcgcga aggcgtccag gcggctgtcg ctgcggatgt
     3301 acttctgggc ctcgcccgcg aagacgccgg cccaggtgag gcgctggctc gcgtagtagc
     3361 ccttcccggg gttgtaccag aggtcgaggt cgttcaggta gccgcggacg aggaccttgt
     3421 tcgtgaggag ccagagcccc tcggcgtcgc ggagctcctt gaggtagggc cggtacttgg
     3481 agctgtcgta gctcacgttg gagagggtcg aggagaggcc gaggccgaag ccgacgtcgc
     3541 ctatctgcgt cgggatcttg tagccgccgg agaggcccag tgtgacctcc cagttctcgt
     3601 agggcatctt gtaggccgag gagatggagc tgcccgccgc ctcgaactcc gcgagggagt
     3661 cgtaggggtc aggcacgtcc tcgtcgtaga agatcggccc caggatgtcc tggcctaccc
     3721 agagctgctc gtgcttgagc gagaggtcga ttccgccgga gatgcgcttc ccgaagagcc
     3781 agttgtccag gaaggagaag gtgaggtcct gggtgtcggg ggagatgttg gtctcgatgc
     3841 cgaaggtctg gccgttcccc aggaagttct tgtcgttcca cttgatgagg cccgagaccg
     3901 ggaaggtgtc gggctcgccg aggcccgaga gggtcacgcc gaactggatg tcggccgtgc
     3961 tctgctcctc gacgttgatg acgaggtcca tgaggttctc gccgccgccg gggagcatct
     4021 ccggctcgat cgccgagaag tactgcaggt tgtagaggtt gcggaggccc tcgacgatct
     4081 tcgccttgga gaagatgtcg cccacctcca ggggaagctc gcgggccatg acggcctcct
     4141 tcgtcttctt gttccccttg aaggtgatgc tctctatgtg ggcgcggtcg cgctccacga
     4201 tgcgcaccgt gtaggcgatg ctgagcttct cctcgtcgcg gcgctcggcc aggtccaggc
     4261 tgttgaagat gtagccgttc tcgtagtaga ggtcgtcgat cctctgcttg tcctgcatga
     4321 gcttgccgta gttgaggatc ccgccctcct tctggaagac gagggcggcg agcttgtcgg
     4381 agccgaagat ctcgttcccc tcgaaggcca tgccggcgta ggtccactgg cggccctcct
     4441 tcagggcgaa ggtgaggacg agccagttct tgccgctctt ctcgtccttc tggacgctcc
     4501 gcacgacgtc ggcgaccgcc gcgtccacgt agccgcgggc gcggtagtag gccacgacct
     4561 tctgcttgtc ctcctcgagc ttgagctcct ggaaggcgcc ggcctggaag aggcccgcct
     4621 ccttgaggga gagctggccc ttgagggtct gggacgagaa ggcgacattg cccgtgaagc
     4681 ggatctcctt gatggcgacc tgcgagccct ccacgacggc gaaggagagg acgaccccgc
     4741 ccgagggccc cgtcgaggtg ctggagctga cctgggcgtc gggatagccc ttctccaggt
     4801 agaggcgcct gacggcgagc tcgtcgagct tggtcttgga ggggttgaag atgtccccgg
     4861 ccttgatctt cacgacgtcg aggatgtcgg agctccggag gcccgagttg ccgacgacgc
     4921 gcacggcgtc cacggcgggc ttctcggtga cggtgaactt gatgaggacc ttggtcttct
     4981 cctcgtccgc cgggaaggcg gcggactgga tggtcgcggg gtcgaaccag tccagctcgt
     5041 agacccgggc ctggagctcc acccagagct cctcggagaa ggccttgccg acgtaggggc
     5101 gcatcgcgga gtcgagttcg cgcttgtcgg cgttcttcac gccctcccac tggacgccgg
     5161 ctatgggctt gccccagaac cagtccgcgg cggcttcctc gccgaaggcc gcgggggcgg
     5221 ccagggagag ggcaagggcg atgaaaaggc agagtcgctt catggtgctc ctcacgtcga
     5281 tcacaatggc agccgccagc tcaggctgag gctctgatcg ctgatgtaga gattctccag
     5341 gctcttggac gtcggcgtga cgctccagag cagggtgccg aagggcgtgt cgaactcgac
     5401 gccgacctcc gagtctaata gcaaaggtcc ggaggggaca agagggtcct cttcccgcag
     5461 gctcaaggag gcgtagagga aggtcctctc gtcgaggtat ttccccgcga agagcgaggt
     5521 ctcgtcgagg taggagccga ggcccgtcgg ctcggtcccg cggtccaggc tcgagatgtc
     5581 gaggagccag cgctgtacga cctggctccg cagggagaag atgtccaggc ccagggcttg
     5641 ccgcacgcgg ctctcgaagg ccttcgtcgc gttgagctgg gggatgaact ccgagctcgc
     5701 gatgacggcc ttgcggatgt ccacctctcc gctctcggag acgccgagga ggccctcccc
     5761 gagcagggcc gcgatctcct cctcggagag gctcgggctc gaggagagct ggggcttgaa
     5821 attggaggcc ggggcgttct cggccctgag catgacgagg atcggccccc tgtcgttacg
     5881 ctcccgccgc tcggcgtaga gggtgacgag ggggtcgaaa tgccccgagg tctcgttgaa
     5941 gacgatcttg gcgctcttca ggaagaaatt gcgctggatg tagaacacgt cgccgccccg
     6001 gaggaaggcc gtacccttga gggacacctc ctgggcctcg ccgaagagct tgagcgcgag
     6061 gcggctcgag ggctccgcgt agcccgagac gatgggcagg tcctcgctcg ggaagaggac
     6121 ctgcacgccc gtgccgaagc cgatgtccag gtcgaggtcg aagaaggtct ggggcttgcg
     6181 cctggagccc tccgccttga agaggccggg attgatgacg acccgggacc tctcgacgct
     6241 cacctttccg ccgagcctgg ccacgtctcc cgaatagctg aagtccaggt ccgcgtaggc
     6301 cgagcccgcg acgaggacgc cgaggatctt cacgtccagg ggcagcggcg cgccgggcag
     6361 gttccgcagg gaggcgctta tctcgccgat cctccagccg tcgaggaggg cctcggcccg
     6421 caaggaggcg cgggcagtcc cgatgggcag ggaggggacg gaaagggaga ggcgcttgcc
     6481 gtccacgagc acgggcaggg tcatggggcc gatcttgccg gcgatgatgt ccgggacggt
     6541 gacggcgatg ccgtccaggt tgagcgagcc cgagaactcg ggatcggcca ggctcccgcc
     6601 cacggagagg ctgccttcga gcctcccccc ctccaggccc acggggaggg ggccgaggag
     6661 gccgaggaag ggcgaaaggt cgacgccgag ccccgtgacc tcggcctcga gctttccg
//
LOCUS       DAOOSJ010000153         6698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7051_length_6698_cov_5.384465, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000153 DAOOSJ010000000
VERSION     DAOOSJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7051_length_6698_cov_5.384465"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLG14_08285"
     CDS             complement(<1..898)
                     /locus_tag="PLG14_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOX49166.1"
                     /translation="MSTRSFRPGRGLLALALLVAVLGGLSSCSRRLGWGVVLWTAPEG
                     PLPAGSVVPVYIRSNINKVYVVGSLDGKKKLELPFWQVELFPSKPKAKARVAEFGPAV
                     SLYLIASRDGLPVREKPSNGARRIYRLHEGQSVKALAKVEGEVVKTGDEILPGDWYYV
                     LTDDGSRGYAFSNTMRLFDEATESAAAPALAQASGSPARADLLFSRSWRPEYFQEMLD
                     DGRVDLDLFSARFGLFTDAVRRQVRIELPSVSQVFDYDSLSEEGGSLVFGGTPLRVRF
                     EGERRLVADWSGRSAQALQPEAE"
     gene            1051..2658
                     /locus_tag="PLG14_08290"
     CDS             1051..2658
                     /locus_tag="PLG14_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX49167.1"
                     /translation="MSDSSPSPSALPAGPVAVIEIGSTGIRFVVAQVDGSGGFAILDR
                     AGKPSRIGRDVFTNGYVSREAVRESVAVLLGYKELLKGYGIAPGEAMVIATSALREAA
                     NRDTYVDRIALQTGFRVSVVEDIEENHLMYLAVQHALQDERRVLSRSNAMILEVGGGS
                     TEIMLLRRGRMAASHSLRIGTVRVDELVRGAGTAPAYLRQFLEDNVRTACDLLEEELP
                     LESVKTFIVIGSDARLAAARLASERFEEYSVLGREAFVGFADEVSALSPEDCVAKLRV
                     PWSEAEGVGPGLQIERLFLERTGAESVVVPNVSIREGLLLTVARGPDTEHEAEMRRQV
                     AASAASLGRKFHYDEAHSTHVAALCLSLFDALRPEHGLGERERLLLESAAILHDIGTI
                     VRTSGHHKHSEYIIQNSEIFGLTHGELTIVANVARYHRKAPPSTTHVNFIALPREDRI
                     VVMKLAAILRVADALDRGHDQRLKEVEFERREDRLVIKAAGAARAGAARAEAGPGSAG
                     DFSLERLSLAEKGDLFEDVFGLEPILS"
     gene            2668..4992
                     /gene="ppk1"
                     /locus_tag="PLG14_08295"
     CDS             2668..4992
                     /gene="ppk1"
                     /locus_tag="PLG14_08295"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOX49168.1"
                     /translation="MTRKYFFNRELSWLEFNARVLEEALDHSTPLLERLKFIAIVGSN
                     FDEFFMIRVASLKARVRAGDVLRDGADLSPRETLQAVSKRVREIVGRQYECLVREVLP
                     ALAKEGLAVVRPSSWTQAERRWLETYFVERVAPLLTPLRVEEDKEFPSTGNLRIHAAF
                     LLRPEAGLSEAEARLGTARRSAADSVGTAAAAAGAGDAASATSATATRASAAAAAGHS
                     GAAGAAAAPGAAAVPGAQESADRVAVVQVPPRLERFITLPREGEGLRLALLDDVVAAF
                     GHRLFPGYGLKESLVFRVTRDADIGVDEDRDDDFIAAMEEIIVDRQNSYPVRLTVTAD
                     SEALAERLRLSLGLEEEEVYTLPGPVDLRGFMELASIDESRFAGADRLRFEPWRPVRP
                     SAPPEGSTIWDEMKKADSILHVPYESFDPVVRFLEDAAQDPAVLAIKMTLYRTSGNSP
                     ILKALTKAAQNGKQVAVLVEVKARFDEERNIAWASRLEQAGAIVVYGLARLKVHAKAA
                     LVVRREEDGSIRRYLHLSTGNYNDKTARLYADLSLFTANEDLCREASIFFNMVTGYSA
                     VQELRFLAVAPFDLKSRVIAMIEREAQRSSPESPGLVVAKMNALVDEEVVSALYRASS
                     AGVRVMLNVRGVCALVPGVKGLSENIVVVSVVGRYLEHARALYFRNGGAEELYLSSAD
                     WMPRNLERRIELMFPILDEGLRAGLRELLLAYFRDNRKAHRLGPSGRWKKVKPAEGEA
                     PFSAQAWFCESVKKRRRVYDEPAESELQVRRKPS"
     gene            5047..5592
                     /locus_tag="PLG14_08300"
     CDS             5047..5592
                     /locus_tag="PLG14_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOX49169.1"
                     /translation="MKKVCVLLADGFEEVEGLTPIDYLRRAGFEVRVAGVTGKDVAGG
                     HDIRVDTDITVDELDEDFDAIVVPGGGQGSRNLAADPDAVALIKRHFAAGKLVAAICA
                     APVVVLHAACGILAGRRFTCFPGLEKDAPGALFSEGRVVVDGNLITARAAGCAGEFSR
                     AVVEALAGPEAAKRLAETVLL"
     gene            complement(5607..6053)
                     /locus_tag="PLG14_08305"
     CDS             complement(5607..6053)
                     /locus_tag="PLG14_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOX49170.1"
                     /translation="MTERGTVTEIRDRLVTVQLEMTAACGACGNSGCKASRNCLQAFN
                     KDDVKIAEGDEVEIEIEGKAQLQGALWVLGLPLLLFGGGYALGRALAPGANEAPAALA
                     GRAGLAVGVVVGVLVQKGKRLDTLPRILRVVDAEAGGPAEVEAVEP"
     gene            complement(5995..>6698)
                     /locus_tag="PLG14_08310"
     CDS             complement(5995..>6698)
                     /locus_tag="PLG14_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOX49171.1"
                     /translation="QHSVYLRKLGLDVMTLGECCYYKRDIVEQLGKASYLIRPANYPY
                     GNPGRGYRVYSTPKGKVAVLQLLGQAGFPRVHLANPFQAVTDLARKLKEETKVVILDF
                     HAATTAEKEAMARHADGLVSAVIGSHGKTLTADARILAGGTAAITDAGRTGSLMSVGG
                     QDAATRIGEFLTGIPAWSKDGTAGLELQGCLVEIGDDGRASSIKALRIPCEEVLDDRT
                     GDGDGDSGQAGDRPA"
BASE COUNT          976 a         2471 c         2311 g          940 t
ORIGIN      
        1 cctccgcctc gggctggagg gcctgggcgc tccgccccga ccagtcggcg accaggcggc
       61 gctccccctc gaagcgcacc cgtagcggcg tgcccccgaa gaccagggat ccgccctcct
      121 cggagaggga gtcgtagtcg aagacctggg agacggaggg gagctcgatc cgcacctggc
      181 ggcggacggc gtccgtgaag aggccgaagc gggcggagaa gaggtccagg tcgacgcggc
      241 cgtcgtccag catctcctgg aagtactcgg gccgccagga gcgcgagaag aggaggtcgg
      301 cgcgggccgg cgagcccgag gcctgggcca gggccggcgc ggcggcgctc tccgtggcct
      361 cgtcgaagag gcgcatcgtg ttggagaagg cgtagccccg ggagccgtcg tccgtgagga
      421 cgtagtacca gtcgcccggc aggatctcgt cgcccgtctt cacgacctcg ccctcgacct
      481 tggccagggc cttcacggac tggccctcgt ggaggcggta gatcctgcgc gcgccgttgg
      541 agggcttctc gcggaccggg aggccgtccc gcgaggcgat gaggtagagg ctgacggccg
      601 ggccgaactc ggccacgcgg gccttggcct tgggcttgga ggggaagagc tcgacctgcc
      661 agaagggcag ctcgagcttc ttcttgccgt ccagggagcc gacgacgtag accttgttga
      721 tgttcgagcg gatgtagacc ggcacgacgg agccggccgg gagggggccc tcgggggccg
      781 tccagaggac gacgccccag cccaggcggc gggagcagga ggagagcccg cctaggacgg
      841 cgacgagaag ggctagggcg aggaggcccc gcccgggacg gaacgaccgg gtcgacatag
      901 tgtacaagtc tacacggatg ccgccgccag aggcaagcga ggcccgcggg ccccgcgact
      961 cgttgcgatc cgggccgcgg cgttgtatgc ttgtgtattg cgcccgccgc cccgccacga
     1021 gggccgccgg gcgggaaagc gaaggctccc atgagcgatt cctcaccctc cccgtccgcc
     1081 ctccccgccg gccccgtcgc cgtcatcgag atcggctcca cgggcatccg cttcgtcgtc
     1141 gcccaggtgg acggctccgg cggcttcgcc atcctggacc gggccggcaa gccgagccgc
     1201 atcggccgcg acgtcttcac gaacggctac gtgagccgcg aggccgtgcg cgagagcgtg
     1261 gccgtcctcc tcggctacaa ggagctcctc aagggctacg gcatcgcgcc cggcgaggcc
     1321 atggtcatcg ccacgagcgc cctccgcgag gcggccaacc gggacaccta cgtggaccgg
     1381 atcgccctcc agaccggctt ccgcgtctcc gtcgtcgagg acatcgagga gaaccacctc
     1441 atgtacctcg cggtccagca cgccctccag gacgagcgcc gcgtcctctc ccgctccaac
     1501 gcgatgatcc tggaggtggg cggcggctcc acggagatca tgctcctaag gcgcggacgc
     1561 atggccgcct cccactccct gcgcatcggc accgtccgcg tcgacgagct cgtgcgcggc
     1621 gcgggcacgg ccccggccta cctccggcag ttcctggagg acaacgtgag gacggcctgc
     1681 gacctcctgg aggaggagct gcccctcgag tcggtcaaga ccttcatcgt catcggctcc
     1741 gacgcccgcc tggccgccgc ccggctcgca tccgagcgct tcgaggagta ctccgtcctg
     1801 ggccgcgagg ccttcgtcgg cttcgccgac gaggtctcgg ccctctcgcc ggaggactgc
     1861 gtcgccaagc tccgcgtgcc ctggtcggag gcggagggcg tcgggcccgg cctccagatc
     1921 gagcggctct tcctggagag gactggcgcg gagtccgtcg tcgtgcccaa cgtctccatc
     1981 cgcgagggcc tcctcctcac cgtggcccgc gggcccgaca ccgagcacga ggcggagatg
     2041 cgccggcagg tggccgcgag cgccgcctca ctggggcgga agttccacta cgacgaggcc
     2101 cactcgaccc acgtcgccgc cctctgcctc tccctcttcg acgccctgcg gcccgagcac
     2161 ggcctcgggg agcgggagcg cctcctcctc gagtcggcgg ccatcctcca cgacatcggc
     2221 accatcgtgc ggacgagcgg ccaccacaag cactccgagt acatcatcca gaactcggag
     2281 atcttcggcc tgacccacgg cgagctcacg atcgtcgcca acgtcgcccg ctaccaccgg
     2341 aaggcgcccc cctcgacgac gcacgtcaac ttcatagccc tcccccgcga ggacaggatc
     2401 gtcgtgatga agctcgcggc catcctgcgc gtcgcggacg ccctggaccg gggccacgac
     2461 cagcgcctca aggaggtcga gttcgagcgc cgggaggacc gcctggtgat caaggcggcc
     2521 ggcgccgcgc gggccggcgc ggcgcgggcc gaggccggtc ccggaagcgc cggcgacttc
     2581 tccctcgagc gcctctccct cgcggagaag ggcgacctct tcgaggacgt cttcggcctc
     2641 gagccgatcc tgagctaggg ggccgccgtg acgcggaagt acttcttcaa ccgcgagctc
     2701 tcctggctcg aattcaacgc gagggtcctg gaagaggccc tggaccactc gacgcccctc
     2761 ctggagcgcc tcaagttcat cgccatcgtc ggctcgaact tcgacgagtt cttcatgatc
     2821 cgggtcgcct cgctcaaggc cagggtcagg gcgggcgacg tcctccgcga cggggccgac
     2881 ctctccccgc gggagaccct ccaggcggtc tccaagcgcg tgcgggagat cgtggggcgg
     2941 cagtacgaat gcctggtccg cgaggtcctg cccgccctcg cgaaggaggg gctcgccgtc
     3001 gtcaggccct cctcgtggac ccaggcggag cggcgctggc tcgagaccta cttcgtggag
     3061 agggtcgccc ccctcctcac ccccctgcgc gtcgaggagg acaaggagtt cccctcgacc
     3121 ggcaacctcc gcatccacgc cgccttcctc ctaaggcccg aggcgggcct ctccgaggcc
     3181 gaggcgcggc tcggaacagc aaggcggtcg gccgcggaca gcgtcgggac ggcggccgcg
     3241 gcagcaggcg cgggggacgc ggcaagcgcg acaagcgcca ccgcaacccg cgcgagcgcc
     3301 gccgccgcgg caggccactc aggcgcggcc ggcgcggccg ccgccccagg cgctgccgcc
     3361 gtccccggcg cccaagaatc cgccgaccgc gtggccgtgg tccaggtccc gcccaggctc
     3421 gagcgcttca tcaccctgcc ccgcgagggc gagggcctgc gcctcgccct cctcgacgac
     3481 gtcgtcgcgg ccttcggcca ccgcctcttc ccgggctacg ggctcaagga gagcctggtc
     3541 ttccgcgtga cgcgcgacgc ggacatcggc gtcgacgagg accgggacga cgacttcatc
     3601 gcggcgatgg aggagatcat cgtcgaccgc cagaactcct accccgtccg cctcaccgtg
     3661 accgcggact cggaggccct ggcggagcgt ctcaggctgt cactgggcct ggaggaggag
     3721 gaggtctaca ccctgcctgg gcccgtcgac ctgcgcggct tcatggagct cgcctccatc
     3781 gacgagtccc gcttcgccgg agccgaccgc ctccgcttcg agccctggcg ccccgtccgc
     3841 ccctcggccc cgcccgaggg ctcgaccatc tgggacgaga tgaagaaggc ggactcgatc
     3901 ctccacgtgc cctacgagtc cttcgacccc gtcgtccgct tcctggagga cgcggcccag
     3961 gatccggccg tcctcgccat caagatgacc ctctaccgca cctcgggcaa ctcgcccatc
     4021 ctcaaggccc tgaccaaggc cgcgcagaac ggcaagcagg tcgccgtcct cgtggaggtg
     4081 aaggcgcgct tcgacgagga gcgtaacatc gcctgggcct cccggctcga gcaggccggc
     4141 gcgatcgtcg tctacggcct cgcgcggctc aaggtgcacg cgaaggccgc cctggtcgta
     4201 cggcgcgagg aggacgggag catccgccgc tacctccacc tctccacggg caactacaac
     4261 gacaagacgg ccaggctcta cgccgacctc tccctcttca cggccaacga ggacctctgc
     4321 cgcgaggcgt ccatcttctt caacatggtc acgggctact ccgcggtgca ggagctccgc
     4381 ttcctcgcgg tggccccctt cgacctcaag tcgcgcgtca tcgcgatgat cgagcgcgag
     4441 gcccagcgct ccagcccgga gtcgcccggc ctcgtcgtcg cgaagatgaa cgccctggtc
     4501 gacgaggagg tcgtctccgc cctctaccgg gcgagctccg ccggcgtcag ggtcatgctc
     4561 aacgtgcgcg gcgtctgcgc cctcgtgccc ggcgtgaagg gcctctcgga gaacatcgtc
     4621 gtcgtgagcg tcgtgggccg ctatctggag cacgcgcggg ccctctactt caggaacggc
     4681 ggcgccgagg agctctacct ctcgagcgcg gactggatgc cgcgcaacct ggagcgccgg
     4741 atcgagctca tgttcccgat cctggacgag ggcctgcgcg cgggcctccg cgagctcctt
     4801 ctcgcctact tccgcgacaa ccggaaggcc caccgcctgg gcccctccgg ccggtggaag
     4861 aaggtcaagc ccgcggaggg cgaggcgccc ttctcggccc aggcctggtt ctgcgagagc
     4921 gtgaagaagc ggcgccgggt ctacgacgag cccgcggaga gcgagctcca ggtcaggcgg
     4981 aaaccgagct agcgccttgc gggccgcggg gcgcggacct agaatcgaag cgtaggagga
     5041 cgaaaggtga agaaagtctg cgtgctcctg gccgacgggt tcgaggaggt cgaggggctc
     5101 accccgatcg attacctcag gcgggcgggc ttcgaggtcc gcgtcgcggg cgtcaccggg
     5161 aaggacgtgg cggggggcca cgacatccgc gtcgacaccg acatcaccgt ggacgagctc
     5221 gacgaggact tcgacgccat cgtggtcccg ggcggcgggc agggctccag gaacctggcg
     5281 gccgaccccg acgccgtcgc cctcatcaag cggcatttcg ccgcgggcaa gctcgtcgcc
     5341 gccatctgcg ccgcgcccgt cgtcgtcctc cacgcggcct gcggcatcct cgcggggcgg
     5401 cgcttcacct gcttcccggg cctcgagaag gacgcgccgg gcgccctctt ctcggagggg
     5461 cgcgtcgtcg tggacggcaa cctcatcacc gcccgcgcgg ccggctgcgc gggggagttc
     5521 tcccgcgccg tcgtcgaggc cctggccggc cccgaggccg cgaagaggct cgccgagacg
     5581 gtcctcctct aggccctaag gtatccctag ggctcgacgg cctcgacctc ggccgggccg
     5641 ccggcctcgg cgtccacgac gcggagaatg cgcggcaggg tgtccaggcg cttccccttc
     5701 tgcacgagga cgccgacgac gacgccgacg gccagtccgg cccgacccgc gagggcggcc
     5761 ggcgcctcgt tggcgccggg cgcgagcgcc cggcccaggg cgtagccgcc gccgaagagg
     5821 aggaggggaa gaccgaggac ccagagggcg ccctggagct gggccttgcc ctcgatctcg
     5881 atctcgacct cgtcgccttc cgctatcttg acgtcgtcct tgttgaaggc ctggaggcag
     5941 ttgcggctcg ccttgcagcc cgagttgccg caggctccgc acgcggccgt catctcaagc
     6001 tggacggtca ccagcctgtc ccgaatctcc gtcaccgtcc cccgttcggt catcgagcac
     6061 ctcctcgcag ggtatcctga gcgccttgat ggacgaggcg cgcccgtcat cgcctatctc
     6121 gaccaggcaa ccctgcagct ccaggccggc cgtgccgtcc ttggaccagg cggggatgcc
     6181 cgtgaggaat tcgcctatcc gcgtcgccgc gtcctgcccg cccaccgaca tgaggctccc
     6241 cgtgcgcccg gcatccgtga tggccgccgt cccccctgcc aggatccgcg cgtcggcggt
     6301 gagggtcttc ccgtgggagc cgatcaccgc cgagacgagg ccgtccgcgt gcctggccat
     6361 cgcctccttc tccgccgtcg tggccgcgtg gaagtcgagg atgacgacct tcgtctcctc
     6421 cttgagcttc cgagcgaggt cggtgacggc ctggaaggga ttggcgaggt ggacccgcgg
     6481 gaagccggcc tggcccagga gctggagcac cgcgaccttg cccttgggcg tcgagtagac
     6541 gcggtagccc cggccggggt tcccgtaggg atagttggcc ggccggatga ggtaagaggc
     6601 cttgccgagc tgctcgacga tgtcgcgctt gtagtagcag cactcgccca gcgtcatcac
     6661 gtccaggccg agcttgcgga ggtagaccga gtgctgcc
//
LOCUS       DAOOSJ010000154         6695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7057_length_6695_cov_6.132229, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000154 DAOOSJ010000000
VERSION     DAOOSJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7057_length_6695_cov_6.132229"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..514)
                     /locus_tag="PLG14_08315"
     CDS             complement(80..514)
                     /locus_tag="PLG14_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49172.1"
                     /translation="MALAHENLYESGDSGGVELASYLGELAARAAVEFGPSLAPAMDL
                     ASATADLESAVFCGLVLSEEIAEAASVSGGGGRVSLSLRLLEPALAEARLRVEGGLKA
                     VGPDGIVAALAGGDDSRVRVETGDGWREVAVRIKVSGGRQES"
     gene            complement(524..964)
                     /locus_tag="PLG14_08320"
     CDS             complement(524..964)
                     /locus_tag="PLG14_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX49173.1"
                     /translation="MRYHRGGNDTGGRGRGIKKGKSRSGAALRVSLIYSAFASAWILG
                     SDRVLLLFSDDPDRLVWVSTAKGWLFVVVTALLLFALVGGEISRRLALEGEIARRLEE
                     KEALLREIHHRVGNNLQVIASLLSLERAGLEGKSCGEVIDGIEA"
     gene            1037..1753
                     /locus_tag="PLG14_08325"
     CDS             1037..1753
                     /locus_tag="PLG14_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX49174.1"
                     /translation="MRELEGLSMIVTGASSGLGRELARQAKARGARLALCGRSEERLR
                     ALAAELGGEAEDGLYLEAFDATDRSAVSAFVARAAGRLGPAEVLVNCAGANTARSRVL
                     DMAPADLDDMLGINLFAPLFFAQAACPAMRAAGRGLVVNILSTACLFSNEGIGAYTAS
                     KRALEGLAGVLRKELRAEGIRVSSVYPGGIDTPFRSASRPDYLEARSVAEAVLALVGM
                     DESVAPDELVLRPLVESNYA"
     gene            1766..2179
                     /locus_tag="PLG14_08330"
     CDS             1766..2179
                     /locus_tag="PLG14_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOX49175.1"
                     /translation="MVLVSACLAGVPCRWDGADKENAKAVELARRGEAVLVCPEQLGG
                     LPTPREPSELSGGRVLDRAGRDVTEEFRRGAEACLAIAELYGCRKAILKAKSPSCGSG
                     LVYDGTFSGRLVPGDGITAALLKSRGIEVVSEEEP"
     gene            2179..2883
                     /locus_tag="PLG14_08335"
     CDS             2179..2883
                     /locus_tag="PLG14_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOX49176.1"
                     /translation="MDLLYLDDAYLRSAEAEVEAVDAARGLARFSRSVVYPGGGGQPR
                     DEGRFSADGASYAIAGAVKEGDEVWYSFEGPLPREGARGLLEIDWDRRYALMRTHSLM
                     HVLCGLVWNEYGKAVTGGNMEPLRARMDFDFPELDLAVLARLEERVRAEIAAARPIRV
                     SWLGRDADLSGLIRTKTNLVPEGLGRIRLVEIEGLDRQADGGTHVARTDEIGAARLTG
                     LENKGKGNKRIRVELA"
     gene            2911..3318
                     /locus_tag="PLG14_08340"
     CDS             2911..3318
                     /locus_tag="PLG14_08340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49177.1"
                     /translation="MLKVEALGPEGLEELLEMLRERAAWLESRGQPMWDPAYLEKDRF
                     VERYRGPECFLAREDGRAVGGFVLVEEDPEIWPEGRGDRAFHVHKLVVRLGEGGKGYA
                     GRLVSWIREFARARGKDFVRLDYYEDRECLARL"
     gene            3396..4004
                     /locus_tag="PLG14_08345"
     CDS             3396..4004
                     /locus_tag="PLG14_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOX49178.1"
                     /translation="MSGPARALGPFPEGGELRGERLEGRDFSSGSLAGLSFAGCSFEA
                     CDFSDCDLSDASFEACSFRGCNLSNPSIKGARFEDCGFEGCKLAGLNFYHCGQLAFDF
                     SFDACRLVGCNFSGLKLKRSRFPACELVDCDFQDCFLVEADFARSRFERTLFHGADLR
                     KADFRGARGYSIDPRTNKVAKAVFSVPDVLGLLAGFDIVVDD"
     gene            4098..4580
                     /locus_tag="PLG14_08350"
     CDS             4098..4580
                     /locus_tag="PLG14_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49179.1"
                     /translation="MESGREGIEIRRAVRTDAPGLLAIQRACFLPDLEKYGDEATNPA
                     NESLERLERKIEACLYFAVLEEGELIGGIDVRRRDERRYRLNRIFIDPARQDLGIGRF
                     CVRRIEELFPEAEEWDLDTPHLSFRNYHLYESLGYVKVGESRQEGSLVLYEYRKAMAR
                     "
     gene            4726..5142
                     /locus_tag="PLG14_08355"
     CDS             4726..5142
                     /locus_tag="PLG14_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49180.1"
                     /translation="MYITNHLVNDRAEMREIFDRITFHQRVYAPDEEELFFCVMDEDK
                     VVGCATLLDASPASAVLEEVVLLAEYAGAEIERGLLSYIVFELEAMGKEELVLGVEGL
                     GEGSGVLSWISGLRVEDRGGKKVLRLKAALSMARAG"
     gene            complement(5199..5840)
                     /locus_tag="PLG14_08360"
     CDS             complement(5199..5840)
                     /locus_tag="PLG14_08360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX49181.1"
                     /translation="MRVLALPGRPKALLFDLDSTLYTNPAYAAFQDEALVGRLARERG
                     ESPAAARAALAGLRAAREASGGGRTSLGNLFAGLGVPIQTSVRWREELFRPAEWLSPD
                     PRLDGTLAALGKRFALALVTNNPRSVGELSLEALGVRGRFSLVVGLDDTLRSKPDPAP
                     FAFAAKGLGLEPGDCVSVGDREDVDLRPALALGMGAVLVEGVEDVYALEAVLP"
     gene            complement(5840..>6695)
                     /gene="prs"
                     /locus_tag="PLG14_08365"
     CDS             complement(5840..>6695)
                     /gene="prs"
                     /locus_tag="PLG14_08365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOX49182.1"
                     /translation="RQHKKKGREGLTAARVSQMLENLGVSRIITLDIHSREIENAFNR
                     MRLENLHASLQIIEKLIGIIDISSSDLVVLSPDTGAVDRNKFYANTLHKPLALLYKER
                     DYSKVSKNAAESNITDMRLLGDVAGKTVFMADDMIGTGGTLLKGMKSIKEMGASRIIC
                     AASLPLFSGSAIEDFDAAYRQGLFYRIIGTNAVHHRELLQKEWYVQADVTGLFAQIIS
                     LLHHNRSLSSMLDNRDLVAKRIKRQLERQPKAPRGAAPQASSGVPLDEEPLPHGALRE
                     IGGPSVQD"
BASE COUNT          960 a         2290 c         2461 g          984 t
ORIGIN      
        1 ggtctcgcgg cagggccggg cggtccggcc gcgaggcccg cgcggcggtc agccccgacc
       61 gaagcccgcg tccggacgcc taggattcct ggcgccctcc cgagaccttg atccgcaccg
      121 ccacctcccg ccagccatct cccgtctcca cgcgcacccg gctgtcgtcg cctcccgcga
      181 gggcggcgac gatgccgtcc ggaccgacgg ccttcagccc tccctccacg cggaggcggg
      241 cctcggccag ggccggctcg aggaggcgca gggagagcga gactcggccg cctccgccgg
      301 agaccgaggc ggcctccgcg atctcctccg agaggacgag gccgcagaac accgcggact
      361 ccaggtcggc cgtggccgac gccaggtcca tggcgggggc caggctcggc ccgaactcga
      421 cggcggcccg cgcggccagc tcgcccaggt aggaggccag ctcgaccccg cctgagtcgc
      481 ccgactcgta gaggttctcg tgggccaggg ccatggccct gagctaggcc tcgatgccgt
      541 cgatcacctc gccgcaggac ttgccttcca ggccggcgcg ctccaggctg aggaggctcg
      601 cgatcacctg gaggttgttc ccgacccggt ggtggatctc gcggagcagg gcttccttct
      661 cctccaggcg cctggcgatc tcgccctcca gcgcgagccg gcgggagatc tcgccgccca
      721 cgagggcgaa gaggaggagg gccgtgacga cgacgaagag ccagcccttg gccgtggaga
      781 cccagaccag gcggtccggg tcgtcggaga agaggaggag gacgcggtcc gaccccagga
      841 tccaggccga ggcgaaggcc gagtagatga gggagacgcg gagtgccgcg ccgctcctcg
      901 attttccttt tttgatgcct ctcccccttc cgccagtatc gttccctcct cgatgatagc
      961 gcaagaccgg acggcgtgcc gggacaaagc cggcctgtac gctgtatcct tggggcggga
     1021 aagggagggc caagccatgc gcgagctcga ggggctgtcg atgatcgtca cgggggcgag
     1081 ctccgggctc ggccgggagc tggcgcgcca ggcgaaggcc cggggtgcgc ggctggccct
     1141 ctgcggccgc tccgaggagc ggcttcgcgc cctcgcggcg gagctgggcg gcgaagccga
     1201 ggacgggctc tacctcgagg ccttcgacgc gacggaccgc tcggccgtct cggccttcgt
     1261 ggcccgcgcc gccgggcgtc tgggcccggc cgaagtcctc gtgaactgcg ccggggccaa
     1321 cacggcccgc tcccgcgtcc tggacatggc gccggccgac ctggacgaca tgctcgggat
     1381 caacctcttc gcgcccctct tcttcgccca ggccgcttgc cccgcgatgc gcgccgccgg
     1441 ccgcggcctc gtcgtaaaca tcctctcgac ggcctgcctc ttctcgaacg agggcatcgg
     1501 ggcctacacc gccagcaagc gcgccctgga gggcctggcc ggcgtcctgc ggaaggagct
     1561 ccgggccgag gggatccgcg tctcctcggt ctatccgggc ggcatcgaca cgcccttccg
     1621 ctccgcctcc cgccccgact acctggaggc gcggagcgtc gcggaggccg tcctggccct
     1681 cgtcgggatg gacgagtccg tcgcgcccga cgagctcgtc ctgcgtcccc tcgtggagtc
     1741 gaactacgcc tgaaggagga aagccatggt cttggtgagc gcctgcctgg ccggcgtccc
     1801 ctgccgatgg gacggggccg acaaggaaaa cgcgaaggcg gtcgagctag cgaggcgcgg
     1861 cgaggccgtc ctggtctgcc ccgagcagct cggcggcctt ccgacgccgc gcgagccctc
     1921 ggagctctcc gggggcaggg tcctggaccg cgcgggcagg gacgtgacgg aggagttccg
     1981 gcgcggcgcg gaggcctgcc tcgcgatcgc cgagctctac ggctgccgca aggcgatcct
     2041 caaggcgaag tcgccctcct gcggctcggg cctggtctac gacgggacct tctcgggcag
     2101 gctcgtcccg ggcgacggga tcacggccgc cctcctcaag tcgcgcggga tagaggtcgt
     2161 ctccgaggag gagccctgat ggacctgctc tacctggacg acgcctacct caggtcggcc
     2221 gaggccgaag tcgaggccgt cgacgccgcg cgcggcctcg cgcgcttctc ccggagcgtc
     2281 gtctaccccg gcgggggcgg ccagccccgc gacgaggggc gcttctccgc cgacggcgcc
     2341 tcctacgcga tagccggcgc cgtgaaggaa ggcgacgagg tctggtactc cttcgagggg
     2401 ccgctccccc gcgaaggcgc gagaggcctc ctcgagatcg actgggacag gcgctacgcc
     2461 ctcatgcgga cccacagcct gatgcacgtc ctctgcggcc tcgtctggaa cgagtacggg
     2521 aaggccgtga cgggcggcaa catggagccg ctcagggcgc ggatggactt cgacttcccc
     2581 gagctcgacc tcgccgtcct cgcgcggctg gaggagcgcg tccgggcgga gatcgcggcc
     2641 gcgcggccga tacgggtctc ctggctcggc cgcgacgccg acctctcggg cctcataagg
     2701 acgaagacga acctcgtgcc cgagggcctg ggcaggatca ggctcgtgga gatcgagggg
     2761 ctggacaggc aggccgacgg cggcacccat gtcgcccgca cggacgagat cggggccgcg
     2821 cgcctaacgg gcctcgagaa caagggcaag ggcaacaaac ggatccgcgt cgagctcgcg
     2881 tagggcccgg cttgggcatg ggggatggat atgctgaagg tcgaggcgct cgggcctgag
     2941 gggctcgagg agctcctgga gatgctgagg gagcgggcag cctggcttga gtcgaggggc
     3001 cagccgatgt gggatcccgc ctacctcgag aaggaccgct tcgtcgagcg ctaccgcggg
     3061 cccgaatgct tcctcgcccg cgaagacggg cgcgcggtgg gcggctttgt cctcgtggag
     3121 gaggatccgg aaatctggcc cgagggccgg ggcgaccggg ctttccatgt ccacaagctc
     3181 gtcgtccgcc ttggcgaggg cggcaagggc tacgccggcc gcctcgtctc gtggatccgc
     3241 gagttcgccc gggcgcgggg caaggatttc gtcaggctgg actactacga ggacagggaa
     3301 tgcctggcca ggctctaggc aggatgcggt ttcaggcccg tggcgacctg gaccgctccc
     3361 gacggaacgc gcctcgtgaa gggcgagctg aggccgtgag cggccccgcg cgggccctcg
     3421 ggcccttccc cgagggcgga gagctccggg gcgagcggct cgaggggcgg gacttctcct
     3481 cgggaagcct ggcaggcctg tccttcgccg gctgctcctt cgaggcctgt gatttctcgg
     3541 actgcgacct ctcggacgcc tccttcgagg cctgctcctt ccgcggctgc aacctgagca
     3601 atccctcgat caagggcgcg cgcttcgagg attgcggctt cgagggctgc aagctcgcgg
     3661 gcctgaactt ctaccactgc ggccagctcg ccttcgactt ctccttcgac gcctgccgcc
     3721 tggtcggctg caacttctcg ggcctcaagc tgaagcgctc ccgcttcccg gcctgcgagc
     3781 tcgtcgactg cgacttccag gactgcttcc tcgtcgaggc ggacttcgcc cgctcccgct
     3841 tcgagcggac gctcttccac ggcgccgacc tgcgcaaggc cgacttccgc ggggcccgcg
     3901 gctactcgat cgatccgcgg accaacaagg tcgcgaaggc cgtcttctcc gtcccggacg
     3961 tcctcggcct ccttgcgggc ttcgacatag tcgtcgatga ttgagcggcg gccgtggcgg
     4021 cgattccggg ggcgcggcgg cgacgcccgg gggcgcggcg gcgacgcgcc gggcggccgc
     4081 ccgggggagg agagaccatg gagtccggac gcgaggggat cgagatcagg agggccgtcc
     4141 ggaccgacgc gccggggctc ctcgccatac agcgggcctg cttcctgccc gacctcgaga
     4201 agtacggcga cgaggcgacg aatcccgcca acgagagcct ggaacggctg gagcgcaaga
     4261 tcgaggcctg cctctacttc gccgtcctgg aggaaggcga gctcatcggc ggcatcgacg
     4321 tgcgcaggcg cgacgagcgc cgctacaggc tcaaccgcat cttcatcgat cccgcccgcc
     4381 aggacctcgg gatcgggcgc ttctgcgtga ggcggatcga ggagctcttc cccgaggccg
     4441 aggagtggga cctggacacg ccgcacctga gcttccggaa ctaccacctc tacgagtcgc
     4501 tcggctacgt gaaggtgggg gagtcgcggc aggagggctc cctcgtcctc tacgagtaca
     4561 ggaaggccat ggcccgctga aggcgggcgg ggcctgggcg ccgcacccgc gccggccggg
     4621 tcccaccccg ccggccagag aggcccgcga tggcccgctt gcattacttc cgcgggccgc
     4681 tataattgag ccagatgtaa agtcatgcgg ccgcgggagt taaatatgta tatcacgaac
     4741 cacctggtca acgaccgggc agagatgcgc gagatcttcg atcgcataac ctttcaccag
     4801 cgagtctacg ccccggacga ggaggagctc ttcttctgcg tcatggacga ggacaaggtc
     4861 gtcggctgcg ccaccctcct tgacgcgagt cccgcgagcg ccgtcctgga ggaggtcgtc
     4921 ctcctcgccg agtacgcggg cgcggagata gagaggggcc tcctctccta catcgtcttc
     4981 gagctcgagg ccatgggcaa ggaagagctc gtgctgggcg tggaaggcct gggggagggc
     5041 tcgggcgtcc tctcctggat ctccggcctc cgggtcgagg accgcggggg caagaaggtc
     5101 ctgaggctca aggccgcctt gtccatggcc cgcgccggct aggcttctcc gccgcgccgg
     5161 ccggcccgcc gcagagccgg cttcccttca gcccgccctc agggcaggac agcttccagc
     5221 gcatagacat cctccacgcc ctccacgagc acggccccca tcccgagggc cagggccggc
     5281 cgcaggtcca cgtcctcgcg gtcgccgacg gagacgcagt ccccaggctc caggcccagg
     5341 cccttcgcgg cgaaggcgaa gggcgcgggg tcgggcttgg agcgcagggt gtcgtccagg
     5401 ccgacgacga gggagaagcg gccgcgcacg cccaaggcct cgaggctcag ctcgccgacc
     5461 gagcgggggt tgttcgttac gagggccagg gcgaagcgct tcccgagggc ggcgagggtc
     5521 ccgtccaggc gggggtcggg ggagagccat tcagccggcc tgaagagctc ctcgcgccag
     5581 cggacgctcg tttggatcgg gacgcccagg cccgcgaaga ggttccccag gctcgtgcgg
     5641 cctccgccag aagcctcgcg ggcggcgcgg aggccggcca gggcggcccg agccgcggcc
     5701 gggctctcgc cgcgctcccg ggcgaggcgc ccgaccaggg cctcgtcctg gaaggcggcg
     5761 taggcggggt tcgtgtagag ggtcgagtcg aggtcgaaga ggagggcctt gggccgcccg
     5821 ggcagggcga ggacccgcat cagtcctgga cggagggccc gccgatctcg cggagggctc
     5881 cgtgggggag gggctcctcg tccaggggca cgccggagga ggcctggggc gccgccccgc
     5941 ggggggcctt gggctggcgc tcgagctggc gcttgatgcg cttggccacg aggtcgcggt
     6001 tgtcgagcat cgaggagagg gagcggttgt ggtggaggag gctgatgatc tgggcgaaga
     6061 ggcccgtgac gtcggcctgc acgtaccact ccttctggag gagctcgcgg tggtgcaccg
     6121 cgttcgtgcc gatgatgcgg tagaagaggc cctgcctgta ggccgcgtcg aagtcctcga
     6181 tggcgctgcc ggagaagagg gggagggagg ccgcgcagat gatccgcgag gcgcccatct
     6241 ccttgatgct cttcatgccc ttgaggaggg tgccgccggt cccgatcatg tcgtcggcca
     6301 tgaagacggt cttgcccgcg acgtcgccca ggaggcgcat gtccgtgatg ttcgactcgg
     6361 ccgcgttctt ggagaccttg gagtagtccc gctccttgta gaggagggcg aggggcttgt
     6421 gcagcgtgtt cgcgtagaac ttgttccggt ccacggcgcc cgtgtccggg gagaggacga
     6481 cgaggtcgga ggaggagatg tcgatgatgc cgatgagctt ctcgatgatc tgcaacgagg
     6541 cgtggaggtt ctccaggcgc atccggttga aggcgttctc gatctcgcgg ctgtggatgt
     6601 ccagggtgat gatccgcgag acgcccaggt tctccagcat ctggctcacg cgggcggcgg
     6661 tcaggccctc gcggcccttc ttcttgtgct ggcgg
//
LOCUS       DAOOSJ010000155         6652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7120_length_6652_cov_4.547218, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000155 DAOOSJ010000000
VERSION     DAOOSJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7120_length_6652_cov_4.547218"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLG14_08370"
     CDS             complement(<1..382)
                     /locus_tag="PLG14_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOX49183.1"
                     /translation="MPASNLLLSSSPHAFTPVSTRKIMLAVVIALLPATGYGVYLYGL
                     SALVLVLATVAASVASELLFRAAIKAPRSVGDLSAVVTGLLLALVLPPSTPIWMAVLG
                     AVFSIVVAKEFFGGLGANPFNPALI"
     gene            complement(369..1715)
                     /gene="rsxC"
                     /locus_tag="PLG14_08375"
     CDS             complement(369..1715)
                     /gene="rsxC"
                     /locus_tag="PLG14_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOX49184.1"
                     /translation="MSVKSFKGGAHPPERKDRSSGLPIEYLRDAKSVVIPVNQHFGAP
                     LSPLVKVGDTVRRGQKIADAEGRMTVPVHASISGVVKKIEPRTQSNNVDGLCFVIEAA
                     EVPGGDDFLPPLDGFACGKDEALARIREAGIVGMGGAGFPAHVKLAPSKPIAVVIANG
                     AECEPYLTIDERAMIERPGDVVEGVALTMRIVGAPKGVIAVEDNKAHVVAGLTKAVAE
                     KGRGMDIAVQLLKTKYPQGGEKMLISAVTGKEVPSGGLPMDVGCVVQNIGTLVATVEA
                     FREGKPLIERGLTVSGGACKSPKNLVVPIGTLVSDLAAEGKLEVDEAALARVLYGGPM
                     MGTAVPGYAIPVQKNTSGVLLLSKDEAAHYAERDCIRCGRCMRACSCRLSPALLNQAL
                     KAGDWEEAEKIGLLDCIECGTCSFVCPAHVQLVQRFRVGKQLLRAKKQKEAQNACK"
     gene            complement(1889..>2161)
                     /locus_tag="PLG14_08380"
     CDS             complement(1889..>2161)
                     /locus_tag="PLG14_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOX49185.1"
                     /translation="IEALETYREHAASIMEIYLSSVSNRTNDIMKVLTIMSTIFIPLT
                     FIAGVYGMNFRHMPELEKAWAYPLVWGVMILVALGELVFFKLRKWI"
     assembly_gap    2162..2261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2262..2834)
                     /locus_tag="PLG14_08385"
     CDS             complement(<2262..2834)
                     /locus_tag="PLG14_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOX49186.1"
                     /translation="MSRRRAKKTPKRSFAPPFRSVTAKTAGLPPGSAVYSGPAEPTEA
                     LLSLYAYDASGWRMECPSTVGALLEALDPAKNNWINVNGLSGGAVERLGERLGIHPLV
                     VEDILDPGQRPRVEAFEDSLFVVTKMLTLHSGGEIEYEQVSLLRKGGVVVSFQETPGD
                     CFGPLRERIASGAGRLAKAGPDFLLYSLLDL"
     gene            3017..>4253
                     /locus_tag="PLG14_08390"
     CDS             3017..>4253
                     /locus_tag="PLG14_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOX49187.1"
                     /translation="MKESVSLGTARRIAAWASGLPPAFGSGREGAAAALRRLGSLQID
                     TISVVERAHLHVLWSRAPNAGADPIAALEAEPRLAFEYWSHAAAYLPLEEYRFSLSRM
                     ERIRSQGHEWFRADQGAVDYVLGRIRAEGPLRAQDFAEPMKGPRGWWDWKPAKVALEY
                     LFHSGALAVATRRGFQKVYDLPERALPSDLDARRPSPAEEAARHVDRAGSSLGLFARD
                     EIAYLRKDGLAGIDAELQARVEAGSLVEVEVEAPAGASGSASGRRRGPVRYASPAALE
                     AAAALPARPAPRAFILSPFDPLVIDRRRLARLFGLDYQVECYVPAAKRRFGYFALPVL
                     FRDGFGDFGFAALADARADRRAKVLELRRLSLEPFEAATAPGGPRRRRATLEREFAAA
                     LAAELGRFAAFNGAERVELG"
     assembly_gap    4254..4353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4375..5901)
                     /locus_tag="PLG14_08395"
     CDS             complement(4375..5901)
                     /locus_tag="PLG14_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOX49188.1"
                     /translation="MKLSTRAGRGDAGRKAGGRGDAGLRTAGLRAAALVLALLAGAGG
                     AAGAQAPAQAAGQGGAGKAAAAKRPEVRIVESPEARLRSAASATSRLVAKLEPGESVL
                     VLETAAKAEKLGGVTAPWFRVAKASGEEGWAFGGWLAKAPARGYYKRGELPEGADYDA
                     YLALALRRGERVVAAEDAERVSAGEKGWAWGRVDGDPPFLVVWDRDLGGTPLDEYLPE
                     GLPRILDTRIYFVYAESLEILGEAATADFAALGLETVAERAGEFGVGAAVTLGRHYAV
                     AEDEDSANWAEEMEDYVGSVAFIEELLGEDDWGRPLALVDADGGEWAWRIENMRLDSG
                     DAYAGDYGDEEGYDGDYGADADAESYGMIAEGSLVVLGRHDELGGDPNWNEDMAEFVG
                     KKAKVAELAGSDGSGFLGVRVEGNDWFWRVRNLALSGRGQAGSYGWRVGDEVILGRHR
                     EIEGSSNWAEEMEAYVGMRATITALLGPEGDSAGCFLVSVDADGGEWSWRAENLLPAD
                     "
     gene            6009..>6652
                     /locus_tag="PLG14_08400"
     CDS             6009..>6652
                     /locus_tag="PLG14_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49189.1"
                     /translation="MVRTIHPVILSLRNRPASSAQIARRTVASCLAAAVVAASLPLLS
                     CRTGSDLDSRPLLPPPPGPSAPTLTFSLAADMRGYVGPSEFRGALSALGQVGAGSFML
                     SPGDIDPPAPVRGAIDDILGTSYPWFPIIGNHEAETPEDMTYLLAYDLSGSATTAGAT
                     NFTAGPTGAEETCYSFDAGGAHFAVINEYYDGSSDIGVTLPPDDALAGNISSELL"
BASE COUNT          898 a         2328 c         2230 g          996 t
ORIGIN      
        1 cgatgagggc cggattgaag gggttggcgc ccaggccgcc gaagaactcc ttggcgacga
       61 cgatggagaa gaccgcgccc aggacggcca tccagatcgg cgtggagggc ggaaggacca
      121 gggccaggag gaggcccgtg acgacggccg agaggtcgcc gaccgagcgg ggcgccttga
      181 tcgccgcgcg gaagaggagc tcggaggcca cggaggcggc caccgtcgcc aggacgagga
      241 cgagggccga aaggccgtag aggtagacgc cgtagcccgt cgcgggaagg agggcgatga
      301 ccacggcgag catgatcttc ctcgtggaga cgggcgtgaa ggcgtggggg ctcgaggaga
      361 ggagcaggtt acttgcaggc attctgggcc tccttctgct tcttggcgcg gaggagctgc
      421 ttgcctacgc ggaagcgctg gacgagctgg acgtgggcgg ggcagacgaa ggagcaggtg
      481 ccgcactcga tgcagtcgag gaggccgatc ttctccgcct cctcccagtc gccggccttg
      541 agggcctggt tcagcagggc cggggagagc ctgcaggagc aggcgcgcat gcagcggccg
      601 cagcggatgc agtcgcgctc ggcgtagtgc gccgcctcgt ccttggagag gaggaggacg
      661 ccggaggtgt tcttctggac ggggatggcg tagccgggga cggccgtgcc catcatgggg
      721 ccgccgtaga ggacgcgggc cagggccgcc tcgtccacct cgagcttgcc ctcggcggcg
      781 aggtcggaga ccagggtgcc gatgggcacg accaggttct tcgggctctt gcaggcgccg
      841 cccgagaccg tgaggccgcg ctcgatgagg ggcttgccct cgcggaaggc ctcgacggtg
      901 gcgaccaggg tgccgatgtt ctgcacgacg cagccgacgt ccatcgggag gccgccggag
      961 ggcacttcct ttccggtgac cgcgctgatg agcatcttct cgccgccctg ggggtacttc
     1021 gtcttgagga gctggacggc gatgtccatg cccctgccct tctcggcgac ggccttcgtg
     1081 aggccggcga ccacgtgggc cttgttgtcc tccacggcga tgacgccctt gggggcgccg
     1141 acgatccgca tcgtgagggc cacgccctcc acgacgtcgc ccgggcgctc gatcatcgcg
     1201 cgctcgtcga tcgtgaggta gggctcgcac tcggcgccgt tcgcgatgac gaccgcgatc
     1261 ggcttggagg gggccagctt gacgtgggcg gggaagcccg cgccgcccat gccgacgatg
     1321 cccgcctctc ggatgcgggc cagggcctcg tccttgccgc aggcgaagcc gtcgagaggc
     1381 gggaggaagt cgtccccgcc ggggacctcg gccgcctcga tcacgaagca gaggccgtcg
     1441 acgttgttcg actgggtgcg gggctcgatc ttcttgacga cgccggagat ggaggcgtgg
     1501 accggcacgg tcatgcgccc ctcggcgtcg gcgatcttct gcccgcgcct caccgtgtct
     1561 ccgaccttca cgagggggga gaggggcgcg ccgaaatgct ggttcaccgg gatgacgacg
     1621 ctcttggcgt cgcgcaggta ttcgatcggg aggccgctgg aacggtcctt ccgctcgggc
     1681 ggatgcgcgc cgcccttgaa gcttttcaca gacatggcga aaaggttctc ctttgccttg
     1741 ctttgagcgc agagcttgac cgcctcgccc gaagggggcg cgccggcttg ttcagggggt
     1801 tgaaaatctc gaatatcgta acgcgctcgg cgaaaacggt caagcgacca gtatggtaag
     1861 tcttttctcc gacgctaatt cgatatattt aaatccattt gcggagcttg aagaagacca
     1921 gctcccccag ggccacgagg atcatgacgc cccagaccag gggataggcc caggccttct
     1981 cgagctcggg catgtgcctg aagttcatcc cgtagacgcc cgcgatgaag gtgaggggta
     2041 tgaagatcgt cgacatgatg gtgaggacct tcatgatgtc gttcgtgcgg ttggagacgg
     2101 aggagaggta gatctccatg atggaggccg cgtgctcccg gtaggtctcc agggcctcga
     2161 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaggtcgag gagcgaatac
     2281 aggaggaagt cgggaccggc cttggcgagc cgccccgccc cagaggcgat gcgctcgcgc
     2341 agggggccga agcagtcgcc gggcgtttcc tggaaggaga cgacgacgcc ccccttgcgg
     2401 aggaggctca cctgctcgta ttcgatctcg ccgcctgagt ggagggtgag catcttcgtg
     2461 acgacgaaga ggctgtcttc gaaggcctcg acgcggggcc gctggcccgg gtccaggatg
     2521 tcctcgacga ccaggggatg gatgcccagc cgctcgccga gcctctccac cgcgccgccc
     2581 gacagcccgt tcacgttgat ccagttgttc ttcgcggggt ccagggcctc gaggagggcg
     2641 cccacggtgg aggggcactc catccgccag cccgaggcgt cgtaggcgta gagggagagg
     2701 agggcctccg tgggctcggc gggaccggag tagacggccg agccgggcgg aagaccggcg
     2761 gtcttcgcgg tgacgctgcg gaagggcggg gcgaaggagc gtttcggggt tttcttggcg
     2821 cggcggcggc tcatggccgg aagggtaccc ttccgcgccg gcccggcgca aggcggccgc
     2881 cgcggtccgc ccgcgctgct ctcaccgggc gcggccggcc ttcgcgactc ccctcgggcg
     2941 cgtcggcctt cgcggccggc cctcgcccca ggcgcctctt gcgccgggcg gcccgcctgc
     3001 cctacattcg ttaagcatga aggaatcggt atccctcggg acggcgagga ggatcgccgc
     3061 ctgggcctcg ggccttcccc ccgccttcgg gagcgggagg gagggcgcgg ccgccgccct
     3121 gcgccgcctc ggctccctgc agatcgacac gatctccgtc gtggagcggg cccacctcca
     3181 cgtcctctgg tcccgcgcgc ccaatgccgg cgccgacccg atcgccgccc tggaggccga
     3241 gccccgcctc gccttcgagt actggtccca cgccgcggcc tacctccccc tggaggagta
     3301 ccgcttctcc ctctcgcgga tggagcgcat caggtcccag ggccacgagt ggttccgcgc
     3361 ggaccagggc gcggtcgact acgtcctggg ccggatcagg gccgagggcc cgctgcgcgc
     3421 ccaggacttc gcggagccca tgaaggggcc gcggggctgg tgggactgga agcccgcgaa
     3481 ggtcgccctc gagtacctct tccactcggg cgccctcgcc gtggcgacgc gcaggggctt
     3541 ccagaaggtc tacgacctcc ccgagcgggc cctgccctcg gacctcgacg cgaggcgccc
     3601 gagccccgcg gaggaggcgg cccgtcacgt cgaccgggcg ggctcctccc tcggcctctt
     3661 tgcccgcgac gagatcgcct acctgagaaa ggacggcctt gcgggcatcg acgcggagct
     3721 ccaggcgagg gtcgaggcgg gcagcctcgt cgaggtggag gtcgaggccc cggcgggagc
     3781 ctcgggctcg gcctcgggac gcaggcgcgg gccggtccgc tacgcctcgc ccgccgccct
     3841 cgaggcggcg gccgccctcc ccgcccggcc cgcgccgcgg gcctttatcc tctcgccctt
     3901 cgatcccctc gtcatcgacc ggcgccgcct ggcgcggctc ttcggcctcg actaccaggt
     3961 agagtgctac gtcccggccg cgaagcggcg cttcggctac ttcgccctgc ccgtcctctt
     4021 ccgcgacggc ttcggggact tcggcttcgc ggccctcgcc gacgccaggg ccgaccggag
     4081 ggcgaaggtc ctggagctcc gccgcctttc cctcgagccc ttcgaggcgg cgaccgcgcc
     4141 cggcgggccc cggcgccgcc gggcgaccct ggagcgcgag ttcgcggccg ccctcgccgc
     4201 cgagcttggg cgcttcgccg ccttcaacgg cgcggagcgc gtcgagctcg gccnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagccctc cgcgccctcc ttgcctagtc
     4381 ggcggggagg aggttctccg cgcgccagga ccactcgcct ccgtccgcgt cgacggagac
     4441 gaggaagcag ccggcggagt cgccctcagg ccccagcagg gccgtgatgg tcgcccgcat
     4501 gccgacgtag gcctccatct cctcggccca gttggagctt ccctcgatct cgcggtggcg
     4561 gcccaggatc acctcgtcgc cgacgcgcca gccgtagctg cccgcctggc ctcgcccgga
     4621 gagggccagg ttgcggacgc gccagaacca gtcgttgccc tcgaccctga cgcccaggaa
     4681 gcccgagccg tcggagcccg cgagctccgc gaccttcgcc ttcttcccga cgaactcggc
     4741 catgtcctcg ttccagttcg ggtcgccgcc cagctcgtcg tggcgtccca ggaccacgag
     4801 ggagccctcg gcgatcatgc cgtaggactc ggcgtcggcg tccgccccgt agtcgccgtc
     4861 gtagccctcc tcgtccccgt agtccccggc gtaggcgtcg cctgaatcca ggcgcatgtt
     4921 ctcgatccgc caggcccact cgccgccgtc cgcgtcgaca agggccaggg gcctgcccca
     4981 gtcgtcctcg cccaggagct cctcgatgaa ggcgaccgag ccgacgtagt cctccatctc
     5041 ctcggcccag ttcgcggagt cctcgtcctc ggccaccgcg tagtgccggc ccagggtcac
     5101 ggccgcgccg acgccgaact ccccggcccg ctccgcgacg gtctcgaggc ccaaggccgc
     5161 gaagtcggcc gtggccgcct cgcccaggat ctccagggac tcggcgtaga cgaagtagat
     5221 gcgggtatcg aggatccgcg gcaggccctc gggcaggtac tcgtcgaggg gcgtgccgcc
     5281 caggtcgcgg tcccagacga ccaggaaggg cggatcgccg tccaccctgc cccaagccca
     5341 gcccttctct ccggcggaga cgcgctcggc gtcctcggcc gcgacgaccc gctcgccgcg
     5401 gcgcagggcc agggcaaggt aggcgtcgta gtcggcgccc tcgggcagtt cgccccgctt
     5461 gtagtagccc cgcgccggcg ccttcgcaag ccagcccccg aaggcccagc cctcctcgcc
     5521 ggaggccttc gccacgcgga accagggggc ggtgacgccg ccgagcttct cggccttggc
     5581 cgccgtctcc aggacgagga cgctctcacc gggctcgagc ttcgccacca agcggctcgt
     5641 tgccgaggcc gcgctccgga gcctcgcctc gggactctcc acgatcctga cctccggccg
     5701 cttggcggcc gcggcctttc cggccccgcc ctgccccgcc gcctgggcgg gcgcctgggc
     5761 gccggcggct ccgcccgcac cagccagaag ggccaggacg agggccgcgg ccctgaggcc
     5821 cgcggtccgc aagcccgcgt cgccccggcc gcccgccttc cgccccgcgt ccccgcgtcc
     5881 cgcgcgcgtc gatagcttca tacaatgctc cttgcttgtc cgctacgctc aactatagag
     5941 ctccggaggt ccgctttcga ggatctcgcc ctttttgcct tgccgaagag gagggatata
     6001 ctatagccat ggtgcgaacc attcatcccg tcatcctttc tctccgaaat agaccggcgt
     6061 cctcagccca aatcgcccgt aggaccgttg cctcctgcct cgccgccgcg gtcgtagcgg
     6121 cttcgctgcc tctcctctcc tgcaggactg gctcggacct cgacagccgg ccactccttc
     6181 ccccgccgcc cggcccaagc gcgccgactc tcaccttctc tctggcggcg gacatgcgcg
     6241 gctacgtggg ccccagtgaa ttccgaggcg ccctctccgc cctcggacaa gtcggcgccg
     6301 gctccttcat gctcagtccc ggcgacatcg atccgcccgc cccggtccgc ggggcgatcg
     6361 acgacattct cggcacctcc tatccctggt ttccgatcat cggcaaccac gaagccgaaa
     6421 ctcccgagga catgacctac ctgcttgcct acgacctttc cggttccgcg actacggccg
     6481 gagcgactaa tttcaccgcc ggcccgacag gcgctgagga gacctgctat tccttcgacg
     6541 ccggcggagc ccacttcgcg gtgatcaacg agtattacga tggatcatcg gatataggcg
     6601 tcacacttcc gcccgacgac gccctcgcgg gcaacatttc ctctgagctc ct
//
LOCUS       DAOOSJ010000156         6647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7126_length_6647_cov_7.286408, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000156 DAOOSJ010000000
VERSION     DAOOSJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7126_length_6647_cov_7.286408"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLG14_08405"
     CDS             complement(<1..1153)
                     /locus_tag="PLG14_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49190.1"
                     /translation="MRATLTFKGRRARGPARSPSSLLPYAAALAAALALAAASPLAAQ
                     EAGGFDEAALFEGAAFETAVAESAAPGGAAAPEFLAGGTIVVRAAASGPVSMDGYSAS
                     SSAQARLFAKASLPDYGAFYAAFNVKQAFLAAGSGGLAPSPAPDLAEPSVTLSELHWS
                     FDLGKVLFLRLGDQLVAWGPSRIWTPVDFVNREKADAFSSVDLRAGKPGLRLHLPLPS
                     ANAFFFADFSGSVEGGAAQDLGESLAYAARLDATLAEAEFGLSGWAGARVQDKLGFDF
                     SGRLFGASVYGEAAWAPAYDSGERSLAASLGLSRALGDLKRWTLGAEFFYNSAGADRT
                     GQYVAPPPDWYAPLYLGEYYGYASLQAKELFSPSLATSLSVLANLSELSY"
     gene            complement(1171..1980)
                     /locus_tag="PLG14_08410"
     CDS             complement(1171..1980)
                     /locus_tag="PLG14_08410"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOX49191.1"
                     /translation="MNARAISKSPALALVLALAAGVLSAAAQGAAGPAPSPLRNEPRA
                     AALVTAFEAKLNLEGLDLANTFTLVQKKEGESDRVLRIRIYRRDSADSFTLVFQFPDS
                     EKGKGYWRSKDDLYLYLPGTREFVYRNRKDDVGSTDVRTDLFGSSRLLDQYWASYSGQ
                     AAVAKWDCDVVRLDAKKLDVSFPLQKWYLRRTDGLPVKVENFSASGTLLRTTYYVDYQ
                     KLGADKYIFTKLLAVNDLEKGQRTFITNEGISTERIADYVFSKAFLEEQSR"
     gene            complement(1992..3317)
                     /locus_tag="PLG14_08415"
     CDS             complement(1992..3317)
                     /locus_tag="PLG14_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX49192.1"
                     /translation="MNRFASVAFRNVFRNGKRSFTLGINYAIVSFILVLLFSFSRGAA
                     STISSSLARATAGHITVSGRFASDGKVFGGLKRKDDIARIARETLGEDAKVIARYQLQ
                     STVYYKGLSKRLAFLGIDATADAGLRGQLAFSEGGWEDWAADPNGLALPADLAEYFGL
                     SLGDEVVVSTRTRFGAFNTGLLKLRGIWKTDNFFLRGQALVAFPFLRELDLATPDAAS
                     TVFVYFPEAKDLAARRDRLSEALASAGFEVSKPKTDSEAIAAVSSASAKYEADEEGRD
                     RIMLKLATLDEVLGLVRNVLGAVNAVGGLVAAVMLFVIAVSIFINLRMSVNERLREIG
                     TMRALGFQAGGVTALFVAESVALALVFSGGGALLGAAAAGAVRGLLRFPPGGNLGIFL
                     EGGRLALLPKPLDMAAIVLAVSLFAALFSYFPARRGGRIRPVEALTRTF"
     gene            complement(3340..4728)
                     /locus_tag="PLG14_08420"
     CDS             complement(3340..4728)
                     /locus_tag="PLG14_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX49193.1"
                     /translation="MLTLKLALRTLARRKGRMILIGSLVAFGTFLVVFGTTVASSASR
                     ASRESIIRNFTGDFIVYSERSRDLPSPFAFNTPLPAIRDSAAVEAALAAMPEVEAYAP
                     YAQNYGLIQAERAGKKLELPFIFYAVDPATYPQVFENAKMEEGAFFAEGTRGVAISRF
                     QNEQYAKNYGATLEVGEPLTLLGITEGGVNTARSSLSGIFSARRYKSVFDYINFMDSA
                     TYAQLYNYSGVKALPADLDAGLAAAALDEAAVFGLADADFGYLDLGALESEALSGSTM
                     IAVRLKAGADADKAMASLLAVPGLGVKAARWDEASGFYAQIAKALQGFIYLATALVFL
                     VVALIFTNTLIINVTERTAEIGTMRAIGAERSFVRGIFLAEALVLNLAASLAAMAAAG
                     AAIAAFGKGGLPLPETVSQFLIGGGPLELRASPLPFLQALAAVLLVSVLATIYPVRVA
                     MSVTPLKAMAER"
     gene            complement(4733..5425)
                     /locus_tag="PLG14_08425"
     CDS             complement(4733..5425)
                     /locus_tag="PLG14_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49194.1"
                     /translation="MKVVELTDVHKHYMLGQTRVDALRGVSLGIEKGEFLAISGPSGS
                     GKSTMLNMFGCIDAPTSGKVVIDGAEVEKLGDKELTRYRRTKIGFIFQSFNLIPVLSV
                     YENIEFPLLLRRDLSAAARKELVTRFVEEVGLGDRMRNKPNELSGGQRQRVAIARALV
                     TGPAIVLADEPTANLDSETGVRIVELMRRINETEKTTFIFSTHDAHIRREARRVVSIL
                     DGRIVGDEGSEG"
     gene            complement(5422..6030)
                     /locus_tag="PLG14_08430"
     CDS             complement(5422..6030)
                     /locus_tag="PLG14_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49195.1"
                     /translation="MLVDKTAGSKARERILDAAEGLFMAKGFAAVTLREICAELGLTH
                     ASLYYHFPGGKEALYAEVAERNISRHGDGLAAALEAGGPRLEGRLKAAAAWLLSQPPM
                     DLLRMARSDLTYLPEEAAGRIARLVYELVLSRLQAALEGAKASGEIGPCDAGLMGGAL
                     VGMVESLHGIPDYALSRSREELAGELIDTLLKGLEYRKGGTS"
     gene            complement(6084..>6647)
                     /locus_tag="PLG14_08435"
     CDS             complement(6084..>6647)
                     /locus_tag="PLG14_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOX49196.1"
                     /translation="VELKRPGMALDFGSLAKGEAAVQAGRLLSARGVRAAVVDVGGSV
                     LVLGSKPGGKPWRVGVQRPGSARGEILGFVEVRDAVVNTSGVYERWFESGGRKWSHAL
                     DARTGWPAESGLVSATVVRGREASADGPSLALLLLGAEEGLALADRLGLAAILVDGER
                     RVHLSRAARPLFTLTDPAYSLVAPQDP"
BASE COUNT         1017 a         2139 c         2532 g          959 t
ORIGIN      
        1 tgtaggagag ctcgctcagg ttcgcgagga cggagaggct cgtcgcgagc gagggcgaga
       61 agagctcctt ggcctggagg gaggcgtagc cgtagtactc gcccaggtag aggggcgcgt
      121 accagtccgg cggcggggca acgtactggc ccgtccggtc ggcgcccgcc gagttgtaga
      181 agaactcggc cccgagggtc cagcgcttca ggtcgcccag ggcccgcgag aggccgaggg
      241 aggccgcaag ggatcgctcg cccgagtcgt aggccggcgc ccaggcagct tcgccgtaga
      301 cgctcgctcc gaagaggcgg cccgagaaat cgaagccgag cttgtcctgg acccgggcgc
      361 cggcccagcc ggagaggccg aactcggcct cggccagggt cgcgtccagc ctggccgcat
      421 aggcgaggct ctcgcccagg tcctgcgccg cgccgccctc gacgctcccc gagaagtccg
      481 cgaagaaaaa ggcgttggcc gagggcaggg ggaggtggag ccgtaggccg ggcttccccg
      541 cgcggaggtc gacggagctg aaggcgtccg ccttttcgcg gttcacgaag tccacggggg
      601 tccagatcct ggagggaccc caggccacga gctggtcgcc gagtctgagg aagaggacct
      661 tccctaggtc gaagctccag tggagctcgg agagggtgac gctcggctcg gccaggtcgg
      721 gggcgggcga gggggcgagg ccgcccgagc ccgcggcgag gaaggcctgt ttgacgttga
      781 aggccgcgta gaaggcgccg tagtcgggga ggctcgcctt cgcgaagagc cgggcctggg
      841 ccgaggagga ggcggagtag ccgtccatcg agacggggcc cgaggcggcg gcgcggacga
      901 ctatggttcc gcccgcgagg aattccgggg cggccgcgcc gcccggggcc gcgctctccg
      961 ccacggccgt ctcgaaggcc gcgccctcga agagggccgc ctcgtcgaag ccgccggcct
     1021 cctgggccgc gagcgggctc gctgcggcca gtgccagggc ggcggccagg gccgcggcgt
     1081 agggcagaag gctggacggg ctccgcgccg gcccgcgggc ccggcggcct ttgaaagtaa
     1141 gcgtcgcgcg catgaccgct cctttcccgc ctagcgggac tgctcctcga ggaaggcctt
     1201 ggagaagacg taatcggcta tcctctccgt ggagatgccc tcgttggtga tgaaggtccg
     1261 ctggcccttc tcgaggtcgt tcaccgcgag gagcttcgtg aagatgtact tgtcggcgcc
     1321 cagcttctgg tagtccacgt agtaggtggt ccggaggagg gtgcccgagg cggagaagtt
     1381 ctccaccttc acgggaaggc cgtccgtccg ccgcaagtac cacttctgga gggggaagga
     1441 gacgtcgagc ttcttcgcgt ccagccgcac gacgtcgcag tcccacttcg cgaccgccgc
     1501 ctggcccgag taggaggccc agtactggtc gaggaggcgg gagctaccga agaggtcggt
     1561 gcgcacgtcg gtgctgccca cgtcgtcctt gcggttccgg tagacgaact ccctcgtgcc
     1621 cgggaggtag aggtagaggt cgtccttgct cctccagtag cccttgccct tctccgagtc
     1681 cgggaactgg aagaccaggg tgaagctgtc cgccgagtcg cggcggtaga tcctgatcct
     1741 gaggacgcgg tccgactcgc cctccttctt ctggacgagg gtgaaggtgt tcgcgaggtc
     1801 caggccctcc aggttgagct tggcctcgaa ggcggtcacg agggccgccg ccctgggctc
     1861 gttccgcagg gggctgggcg cggggcccgc ggcgccctgg gccgcggcgc tgaggacgcc
     1921 ggccgccagg gccaggacga gggcgagggc cggggacttc gaaatggcgc gtgcgttcat
     1981 cgtgctgctc cttagaaggt ccgggtcagg gcctcgacgg ggcggatcct gccgccgcgg
     2041 cgggccggga agtaggagaa gagggccgcg aagagggaga cggcgaggac gatcgcggcc
     2101 atgtccaggg gcttgggaag gagggcgagc ctgccgccct ccaggaagat gcccaggttc
     2161 cccccgggcg ggaagcggag gaggccgcgg acggcgccgg ccgcggccgc gcccaggagg
     2221 gctccgccgc cggagaagac gagggccagg gccacgctct ccgccacgaa gagggccgtg
     2281 acgccccccg cctggaagcc gagggcgcgc atcgtgccta tctcccggag ccgctcgttc
     2341 acgctcatcc tgaggttgat gaagatggag accgcgatga cgaagagcat cacggccgcg
     2401 acgagtccgc cgaccgcgtt caccgcgccg aggacgttgc ggacaaggcc caggacctcg
     2461 tccagggtgg cgagcttgag catgatcctg tccctgccct cctcgtcggc ctcgtacttc
     2521 gcgctcgcgg aggagacggc ggcgatggcc tcggagtcgg tcttgggctt ggagacctcg
     2581 aagcccgcgg aagccagggc ctccgagagc cggtccctgc gggccgcgag gtccttcgcc
     2641 tcggggaagt agacgaagac ggttgaggcc gcgtcgggcg tggccaggtc cagctcgcgg
     2701 aggaagggga aggcgaccag ggcctggccg cgtaggaaga agttgtccgt cttccagatg
     2761 cccctgagct tgaggagccc cgtgttgaag gccccgaagc gggtcctcgt ggagacgacc
     2821 acctcgtcgc ccagggagag gccgaagtac tcggccaggt cggccggcag ggcgaggccg
     2881 ttcgggtcgg ccgcccagtc ctcccagccg ccctcggaga aggccagctg gccgcggagt
     2941 cccgcgtccg cggtcgcgtc gatccccagg aaggccaggc gcttggagag gcccttgtag
     3001 tagaccgtgg actggagctg gtagcgcgcg atgaccttcg cgtcctcccc cagggtctcg
     3061 cgggctatcc gggcgatgtc gtccttgcgc ttgaggccgc cgaagacctt gccgtcggag
     3121 gcgaagcggc cggagacggt gatgtggccg gccgtggcgc gggccaggct cgacgagatg
     3181 gtcgaggccg cgccgcggga gaaggagaag aggaggacga ggatgaagga gacgatggcg
     3241 tagttgatcc cgagggtgaa gctgcgcttg ccgttcctga agacgttccg gaaggcgacg
     3301 ctcgcgaagc ggttcatgtg ctgcctgcct ttcccgcgcc tagcgctcgg ccatcgcctt
     3361 gagcggcgtg acggacatgg cgacgcggac ggggtagatc gtggcgagga ccgacacgag
     3421 gaggaccgcc gcgagggcct ggaggaaggg cagggggctc gcgcggagct cgagcggccc
     3481 gcccccgatg aggaactggg agacggtctc cgggagcggg aggccgccct tgccgaaggc
     3541 cgcgatggcg gcgcccgcgg cggccatggc ggccagggag gccgcgaggt tcaggaccag
     3601 ggcctccgcg aggaagatcc cgcggacgaa ggaccgctcc gcgccgatgg cgcgcatcgt
     3661 gcctatctcc gccgtccgct ccgtcacgtt gatgatgagg gtgttcgtga agatgagggc
     3721 gacgaccagg aacacgaggg ccgtggccag gtagatgaag ccctggaggg ccttcgcgat
     3781 ctgggcgtag aagccggagg cctcgtccca gcgggcggcc ttcacgccca ggccggggac
     3841 ggccagtagg gacgccatcg ccttgtccgc gtcggcgccc gccttgagcc tcaccgcgat
     3901 catcgtcgag ccggagaggg cctcggactc gagggcgccc aggtccaggt agccgaagtc
     3961 cgcgtcggcc aggccgaaga ccgccgcctc gtccagggcc gcggcggcga ggccagcgtc
     4021 caggtcggcg ggcagggcct tcacgccgga gtagttgtag agctgggcgt aggtcgcgga
     4081 gtccatgaag ttgatgtagt cgaagacgct cttgtagcgc ctcgcggaga agatgcccga
     4141 gagcgaggag cgggcggtgt tgaccccgcc ctcggtgatg ccgaggaggg tgaggggctc
     4201 gcccacctcg agggtcgctc cgtagttctt ggcgtactgc tcgttctgga agcggctgat
     4261 cgccacgccc cgggtcccct ccgcgaagaa ggcgccctcc tccatcttcg cgttctcgaa
     4321 gacctgcggg taggtggcgg ggtcgacggc gtagaagatg aagggcagct cgagcttctt
     4381 cccggcgcgc tcggcctgga tgaggccgta gttctgcgcg tagggcgcgt aggcctccac
     4441 ctcgggcatc gccgccaggg ccgcctccac ggcggcggag tccctgatgg cgggcagggg
     4501 cgtgttgaag gcgaagggcg aggggaggtc ccgcgagcgc tccgagtaga cgatgaagtc
     4561 gcccgtgaaa ttgcggatga tggactcgcg ggaggcccgg gacgccgagg aggcgacggt
     4621 cgtgccgaag accacgagga aggtgccgaa ggcgacgagg gagccgatga ggatcatccg
     4681 ccccttgcgc cgcgcgaggg tgcgcaacgc gagcttcagg gtgagcatcg acctagccct
     4741 ccgacccttc gtcgccgacg atccggccgt ccaggatgct gacgacccgc ctggcctcgc
     4801 gcctgatgtg ggcgtcgtgg gtggagaaga tgaaggtcgt cttctccgtc tcgttgatcc
     4861 tgcgcatgag ctcgacgatg cgcacgccgg tctccgagtc caggttggcc gtcggctcgt
     4921 cggccagaac gatggccggg cccgtcacga gggcgcgggc gatggcgacg cgctggcgct
     4981 ggccgccgga gagctcgttc ggcttgttgc gcatcctgtc gcccaggccc acctcctcca
     5041 cgaagcgcgt cacgagctcc ttccgcgccg cggccgagag gtcgcgcctg aggaggaggg
     5101 ggaactcgat gttctcgtag acgctcagca cggggatgag gttgaaggac tggaagatga
     5161 agccgatctt cgtgcggcgg tagcgggtga gctccttgtc gccgagcttt tccacctcgg
     5221 cgccgtcgat caccaccttg ccggaggtag gcgcgtcgat gcagccgaac atgttgagca
     5281 tcgtgctctt gcccgagccc gagggcccgg atatggcgag gaactcgccc ttctcgatgc
     5341 ccaggctcac gccgcgcagg gcgtccaccc gggtctggcc gagcatgtag tgcttgtgga
     5401 cgtcggtcag ttccacgacc ttcatgaggt tcctcctttt cggtactcga ggcctttgag
     5461 gagcgtgtcg atcagctcgc cggccagctc ctcgcggctt cggctgagcg cgtagtccgg
     5521 tatcccgtgc aggctctcca ccatccccac cagggccccg cccatgagac cggcgtcgca
     5581 gggcccgatc tcgcccgaag ccttggcccc ctcgagggcg gcctggaggc gggagaggac
     5641 gagctcgtag acgaggcggg cgatccgccc cgcggcctcc tcggggaggt aggtgaggtc
     5701 cgacctcgcc atccggagga ggtccatcgg gggctgtgag aggagccagg ccgcggcggc
     5761 cttgagccgg ccctcgagcc tggggccgcc cgcctccagg gcggcggcca gcccatcgcc
     5821 gtggcggctg atgttgcgct ccgccacctc cgcgtagagg gcttccttgc cgccggggaa
     5881 gtggtagtag agcgaggcgt gggtcaggcc gagttcggcg cagatctcgc ggagggtcac
     5941 cgccgcgaag cccttcgcca tgaagaggcc ctccgcggcg tccaggatgc gctccctggc
     6001 tttcgatccc gccgtcttgt ctaccaacat attggtaagt tagtggctgt atagtgagaa
     6061 agcaatataa gaatggtgaa atatcacggg tcttggggcg cgaccaggct gtaggccgga
     6121 tccgtgagcg tgaagagggg gcgggcggcc ctggagagat ggacgcgccg ctccccgtcc
     6181 acgaggatgg ccgcgagccc gagccggtcg gcgagggcga gcccctcctc cgcgcccagg
     6241 aggaggaggg cgaggctcgg gccgtcggcg ctcgcctccc ggccgcggac caccgtggcg
     6301 gagacgaggc cggactccgc gggccagccc gtcctcgcgt ccagggcgtg gctccacttc
     6361 ctgccgccgg actcgaacca gcgctcgtag acgccggagg tgttgacgac ggcgtcgcgg
     6421 acctcgacga agccgaggat ctcgccgcgg gccgagccgg ggcgctggac gccgacgcgc
     6481 cagggcttgc ctccgggctt cgagccgagg acgaggacgg atccgcccac gtcgacgacg
     6541 gcggcgcgga cgccgcgggc cgaaaggagc ctccccgcct ggacggccgc ctcgcccttc
     6601 gcgagggagc cgaagtcgag ggccatcccc ggccgcttga gctcgac
//
LOCUS       DAOOSJ010000157         6596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7192_length_6596_cov_5.802477, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000157 DAOOSJ010000000
VERSION     DAOOSJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_6596_cov_5.802477"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLG14_08440"
     CDS             complement(<1..231)
                     /locus_tag="PLG14_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49197.1"
                     /translation="MLLIYPPGARAAEPPLGIARLAGYLREKGRPARCLDLGLEGILY
                     LLGEAAEGTGSAGAAERPGADEPAGRPGVAGPA"
     gene            complement(268..1026)
                     /locus_tag="PLG14_08445"
     CDS             complement(268..1026)
                     /locus_tag="PLG14_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX49198.1"
                     /translation="MDITILGVGGFDNVGLPFNSFLVDGRLLVETPPDILQSLRREGV
                     DPRSIEAVFVSHTHGDHVFGAPFLLFQLWRAREASAGPLRIIGPRDLRATILRLAALA
                     IRDDHPYVRWIEESCSFQVAEAGSRLEVLGYEARFYKMFHEKETCGLGLSQGGRLRLQ
                     YLPDTRWDEGLKAYFAEGADLVLCDVNGTGGNREVHMSLEELESNLAGIIPEGTRILG
                     THLADAIASSSSLVGIAEPGQRLRLLDRAGEPRS"
     gene            1162..1842
                     /locus_tag="PLG14_08450"
     CDS             1162..1842
                     /locus_tag="PLG14_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX49199.1"
                     /translation="MAIFKIMIVEDDPAVRALSSEALAKWGFEVSAPSPEGDVAAEFA
                     RSQPHLVILDVGLPRLDGYEWCARIREVSKAPILFLSARSHPADAVRDLAGGGDDWLT
                     KPFDVEVLVARVKALLRRAYSWAAEATTLLEREGLVLDREGSRASLEGKSVALTRNEA
                     LLLRRLLERGGRVVSRDELQDALWSEDAFVDDNTLTVNMSRLKSALDEIGAKDRIETL
                     RGAGYRLK"
     gene            2040..3050
                     /locus_tag="PLG14_08455"
     CDS             2040..3050
                     /locus_tag="PLG14_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX49200.1"
                     /translation="MALALAFQGGFAVAVMVLDRANRALPSDVLYVVAVESVAAAAFL
                     AWKAARILGQRKRLEAATAAPLEAALPAAQGAAEEAWRDLAEAARREARREAAKREER
                     AKADLEAFLASVHALKTPATALSLMAERAEREGESLKPSELRLEIDELDGILDRTLGR
                     LRLDDFERGSIFRPCDAAQAARASLRKHRRLLIARGIGVHIEGSFTAETDPSWLAFIL
                     DQAVSNAAKYARSRVSVAMRASGRLGTIEIGDDGPGFDPEDASRAFEPSATGSAGRAA
                     GASGAELPASSGYGLHLAAEAARRLGAVLELDSGREGPGGAVLRLVLPLALDARDDLT
                     AP"
     gene            3135..3893
                     /locus_tag="PLG14_08460"
     CDS             3135..3893
                     /locus_tag="PLG14_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49201.1"
                     /translation="MALLEVRGLTKTYAARTGGAARKALDSVSLSVEAGEFVAVMGPS
                     GSGKTTLLNLVALIDEPDSGEILFGSRDPRGLRGSGLAAFRRRNLGYVFQDSALIDTM
                     TLGENIALPLALDRAPGGEIEGRVAAMAERLGISEALGRYPCEVSGGQRQRAASARAL
                     ACGPALLLADEPTGALDSKSGRALMECYASLNASLGNAVLMVTHDPFAASWAMRAVFL
                     RDGKVFSELRRTGGRREFFDRVMEAQAAMEGGGA"
     gene            3890..5863
                     /locus_tag="PLG14_08465"
     CDS             3890..5863
                     /locus_tag="PLG14_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX49202.1"
                     /translation="MTAFGLLAASAGRRTRDSALLAASTAVSAMAIAVFAAAGGSPAF
                     AGIVETRETVRLLFLSASLVMAFFVALSGWFYAGHYLKRRKRELASLLLLGMRKDAVV
                     LLLSLEFAAAAALALAAGLGLAALFSRFFGLCLGALMKGRTPMSLKFDPASAAAAGLA
                     CLAQWTAATARGAWDLSRTSVLGLFKSERETEYGAGPRGAERTGRTGRSWGAGRALAP
                     LGALLLGLSYSGAALAEGPTAERLMIPVLLGTIAGSFLVFEALAPRLVGRFREGGRPR
                     DPALMVAAAQVAFRSRRNARLLAFGSVLVAVAAASLGSVMALNERDEAVARRMCPNGV
                     EFTAADSEGEGAAAAALVEAILSEAGARRWRRVELARIEGTLRTEDGEARPASLFEAE
                     AASRLFAARGEEPVAVEAGRASSFLASRLAGRAYGAESRPVPLSLEAGGHAERLVSYP
                     APSAPPLSLISANAPFLLSTAEWRRFEAAVPAQARLRVYAWNGLGDRELRAARPRLEE
                     AGIPGLLLKPRVLDEQAGQYGILLFIGSFMAATFILAAAALLAFRSIEDARDDAERYR
                     TLLGLGAGMEVVKKSLRLQNAFAFGLPLLLGLCHSAFALAMLKNISGQPNLGPTLTVS
                     AGSAVAFALFAGLAARRQSEAIRADAASRGLAL"
     gene            5998..>6596
                     /locus_tag="PLG14_08470"
     CDS             5998..>6596
                     /locus_tag="PLG14_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX49203.1"
                     /translation="MKRSVTVGLALAVFACLVPPSPSFAEEKTIRFDPLWDPALHAAG
                     FAGAAASRLALEGADRALPDPAELSGFDASLRFDYDEGLSTLSTGASGLAILWPALFA
                     LQGEREEIFPAAAACAEALAMTYAAKNAMKALFPKARPYAYGGGELSAELREEAWESF
                     PSGHAALAFCAATSFAVLSLELAPENPATPWLVAGGYGLA"
BASE COUNT          863 a         2450 c         2318 g          965 t
ORIGIN      
        1 cgccggcccc gcgactccgg gccttccggc cggctcatcg gctccgggtc tctcggccgc
       61 gccggccgaa ccagtcccct cggccgcctc gcccaggagg tacaggatgc cctccaggcc
      121 caggtcaagg cagcgggcgg ggcggccctt ctcgcggagg tagcccgcta gcctggctat
      181 cccgaggggc ggctcggctg cccgcgcccc gggcggataa atgaggagca tctacgggcg
      241 atactaccgt caaggcgggg gcgaggtcta cgatcgaggc tccccggccc ggtccagcag
      301 ccgcagcctc tggcccggct cggcgatgcc gacgaggctc gagctgctcg cgatcgcgtc
      361 cgcgagatgg gtgccgagta tcctcgtccc ttcgggaatg atcccggcga ggttcgactc
      421 gagctcctcg aggctcatgt gcacctcccg gttgccgccg gtcccgttga cgtcgcagag
      481 gacgaggtcc gcgccctccg cgaaataggc cttgagcccc tcgtcccagc gcgtgtcggg
      541 caggtactgc agcctgagtc ggccgccctg ggagaggccg aggccgcagg tctccttctc
      601 gtggaacatc ttgtagaagc gggcttcgta gcccaggacc tcgagccggg agcccgcctc
      661 agcgacctgg aaggagcagc tctcctcgat ccagcggaca tagggatggt cgtcgcgtat
      721 cgcgagggcg gcgagcctca ggatcgtcgc gcggaggtcc cggggcccga tgatcctcag
      781 gggcccggcc gaggcttccc gcgcccgcca gagctggaag aggaggaagg gcgcgccgaa
      841 gacgtggtcg ccgtgggtgt gcgagacgaa gacagcctct atcgaccggg gatctacccc
      901 ttcccgcctg agcgactgga ggatgtcggg aggcgtctcc acgaggaggc gcccgtccac
      961 caggaaggag ttgaagggaa gtccgacatt gtcgaagccg ccgacgccga ggatcgtgat
     1021 atccatgatg acgcatcatc gcaccttggc gcttcatggc gcaatcccgg ccgcggaatt
     1081 caaggcgcga gatccggacc gcgccgtcgc gtagagggag gcctggaaat cgcgggcgcc
     1141 ttgtggcagg attgtcctcc gatggccatc ttcaagatca tgatagtcga ggacgatccc
     1201 gccgtacggg ccctctcctc cgaggcccta gcgaagtggg gcttcgaggt cagcgcgccc
     1261 tctccggagg gcgacgtggc cgccgaattc gcgcgctccc agccccatct cgtcatcctg
     1321 gacgtgggcc tgccccgcct ggacggctac gagtggtgcg ctcggatccg cgaggtctcc
     1381 aaggccccga tcctcttcct gagcgcccgc tcccatccgg ccgacgccgt gcgcgacctg
     1441 gccggaggcg gcgacgactg gctcacgaag cccttcgacg tcgaggtcct ggtggcgagg
     1501 gtgaaggccc tgctccgcag ggcctactcc tgggcggccg aggccacgac gctcctcgag
     1561 agggagggcc tcgtcctgga ccgggagggc tcgcgggcga gcctggaggg gaagtcggtg
     1621 gcgctcacgc gcaacgaggc cctcctcctg aggcggctcc tggagcgcgg cggccgcgtc
     1681 gtgtcccgcg acgagctcca ggacgcgctg tggagcgagg acgccttcgt cgacgacaac
     1741 acccttaccg tcaacatgtc gcggctcaag tccgccctgg acgagatcgg cgccaaggac
     1801 aggatcgaga ccctgcgcgg cgcgggatac cggctcaagt gagggacagg gctggatcgg
     1861 acgcggctgg cgggcggccc ggccgcgccg caggccaagc cgcgggccaa gccgcgggcc
     1921 aggccgcgga cgaggcggga cgagccgccg caagcaaagg ccgagtcgcc gcaagcaaag
     1981 gccgggccgc cgccgaaaac ggccgcgcaa tccgcgcggc cgcgagggac ctggtcccct
     2041 tggccctggc cctcgccttc cagggaggct tcgccgtcgc cgtgatggtc ctggaccgcg
     2101 cgaaccgggc cctgcccagc gatgtcctct atgtggtcgc ggtcgagtcg gtcgcggcgg
     2161 cggccttcct cgcctggaag gccgcgcgga tccttggcca gcggaagcgg ctcgaggcgg
     2221 cgacggccgc tcccctcgag gcggccctgc ccgccgctca aggcgcggcc gaggaggcct
     2281 ggcgggactt ggccgaggcg gccaggcggg aggcgcggcg cgaggccgcg aagcgcgagg
     2341 agagggcgaa ggccgacctc gaagccttcc tggcctccgt ccacgccctg aagacgccgg
     2401 cgacggcctt gagtctcatg gccgagaggg ccgagcggga gggcgagagc ctcaagccct
     2461 cggagctcag gctcgagatc gacgagctgg acggcatcct cgaccggacg ctcggacgcc
     2521 tcaggctcga cgacttcgag cgcggctcca tcttccgccc ctgcgacgcg gcccaggcgg
     2581 ctcgcgcctc tctgcgcaag caccggcgcc tcctcatcgc ccgcggcatc ggggtccaca
     2641 tcgaggggag cttcacggcc gagaccgatc cctcctggct cgccttcatc ctggaccagg
     2701 ccgtctccaa cgcggccaag tacgcccgct ccagggtgag cgtcgcgatg cgggcctcgg
     2761 gacggctcgg gaccatcgag ataggcgacg acggtccggg cttcgaccct gaggacgcct
     2821 ccagggcctt cgagccctcg gccacgggga gcgccggccg ggccgccggc gcctccggcg
     2881 cggagctgcc cgcctcctcg ggctacggcc tccacctcgc ggcggaggcc gcgcggaggc
     2941 tcggcgccgt cctggagctc gacagcggac gggagggtcc gggcggcgcc gtcctgcggc
     3001 tcgtcctccc cctcgccctg gacgcgcggg acgaccttac ggcgccgtaa gggagaaggg
     3061 ccttggcgta agccgtttcg atggatcgcg agcccccgga agcccatact gttccgcgaa
     3121 aggagtcaag aaggatggcg ttgctcgagg ttcgcggcct taccaagacc tacgcggcga
     3181 ggacgggggg agccgcccgg aaggccctcg actccgtctc gctcagcgtg gaggcggggg
     3241 agttcgtcgc ggtgatgggg ccctcgggct cggggaagac gactctcctc aacctggtcg
     3301 ccctgatcga cgagccggac tccggggaga tcctcttcgg ctcgcgggat ccccggggcc
     3361 tccgcggctc cggcctggcc gccttccgga ggcggaacct cggctacgtc ttccaggact
     3421 cggccctgat cgacacgatg accctcggcg agaacatcgc cctgcccctg gccctggacc
     3481 gcgcccccgg cggggagatc gagggccgcg tggcggccat ggcggagcgc ctgggaatct
     3541 ccgaggccct gggccgctac ccttgcgagg tctcgggcgg ccagcgccag cgcgccgcct
     3601 ccgcgagggc cctggcctgc ggccccgccc tcctcctcgc cgacgagccc acgggggccc
     3661 tggactccaa atcgggccgc gccctcatgg agtgctacgc gtcgctcaac gcgtccttgg
     3721 ggaacgcggt cctcatggtg acgcacgacc ccttcgcggc gagctgggcg atgagggccg
     3781 tcttcctccg cgacggcaag gtcttctccg agctcaggcg gacggggggg cgccgcgagt
     3841 tcttcgaccg cgtcatggag gcgcaggccg cgatggaagg cggcggcgcg tgaccgcctt
     3901 cggcctcctc gccgcctccg ccgggcgcag gacgagggac tcggccctcc tcgccgcctc
     3961 cacggccgtc tccgccatgg ccatcgccgt cttcgccgcc gccggcggct ccccggcctt
     4021 tgccggcatc gtggagaccc gggagacggt caggctactc ttcctctccg cctccctcgt
     4081 gatggccttc ttcgtggccc tctcgggatg gttctacgca ggccattacc tcaagcgacg
     4141 gaagcgcgag ctcgcctccc tcctcctcct cggcatgagg aaggacgccg tcgtcctctt
     4201 gctctccctg gagttcgccg ccgcggcggc cctggccctc gccgccgggc ttggcctcgc
     4261 ggccctcttc tcgcgcttct tcggcctctg cctgggcgcc ctcatgaaag gcaggactcc
     4321 gatgtccctg aagttcgacc ccgcctcggc ggccgcggcc ggcctcgcct gcctggccca
     4381 atggacggcc gcgacggcgc gcggcgcctg ggacctgtcg cggacgagcg tcctgggcct
     4441 cttcaagtcc gagcgggaga ccgaatacgg agcgggcccc aggggcgcgg agcggaccgg
     4501 gcggaccggc cgatcctggg gagccggccg ggccttggcg ccgctcggcg ccctcctcct
     4561 tggcctctcc tactccggcg cggccctggc cgaagggccg acggccgagc gcctcatgat
     4621 ccccgtcctc ctcgggacga tagccggcag cttcctcgtc ttcgaggccc tggcgccgcg
     4681 gctcgtcggc cgcttcaggg aaggcggacg gccccgcgat cccgccctca tggtggccgc
     4741 cgcgcaggtc gccttccggt cgaggaggaa cgccaggctc ctcgccttcg gctccgtcct
     4801 ggtcgcggtt gcggcggcct cgctgggctc ggtgatggcc ctgaacgagc gggacgaggc
     4861 cgtggcgcgc cgcatgtgcc cgaacggcgt cgagttcacg gcggcggact cggaggggga
     4921 aggcgcggcc gccgcggccc tcgtggaggc catcctctcc gaggccggcg cgaggcgctg
     4981 gcggcgcgtc gagcttgcta ggatcgaggg gacgctgagg accgaggacg gcgaggcccg
     5041 gcccgcgagt ctcttcgagg ccgaagcggc ttcgagactc ttcgccgccc ggggcgagga
     5101 acccgtcgcg gtcgaggcgg gaagggccag ctccttcctg gcctcccgcc tggccggccg
     5161 cgcctacggg gccgagagca ggccggttcc cctgtcgctc gaggcgggag ggcacgcgga
     5221 gcgcctcgtc tcctatccgg cgccctccgc cccgcccctc tccctcatct ctgccaacgc
     5281 ccccttcctc ctctccacgg cggagtggag gcgcttcgag gcggccgtcc cggcccaggc
     5341 ccggctccgc gtctacgcct ggaacggctt aggcgaccgg gagctccgcg cggcccgtcc
     5401 caggctcgag gaggccggca tccccggact cctcctcaag ccgcgcgtcc tggacgagca
     5461 ggcgggccag tacggaatcc tcctcttcat cggctccttc atggcggcca ccttcatcct
     5521 ggccgcggcg gccctcctgg ccttccgctc gatagaggac gccagggacg acgcggagcg
     5581 ctacaggacc ctcctcggcc tgggcgcagg gatggaggtg gtcaagaagt ccctgcgcct
     5641 gcagaacgcc ttcgccttcg gccttcccct cctcctcggc ctctgccact cggccttcgc
     5701 cctggccatg ctcaagaaca tctcgggcca gccgaacttg ggcccgaccc tgaccgtctc
     5761 cgcgggctca gccgtggcct tcgccctctt cgctggcttg gccgcgcggc gacagtcgga
     5821 ggcgatccgc gccgacgcgg cgagccgggg gctggccctc taggctttcg gcgatgtaag
     5881 cgttttccct ctcatcgtaa ggcagttcga tggctccttc ccgcgcctcg gggctaggct
     5941 cgaggcgtga tttttaggcg cggccgcgca aaggcggtcc cccaaaggaa ggaagccatg
     6001 aaaagatccg tcaccgtcgg cctggccttg gccgtcttcg cctgcctggt cccgccaagc
     6061 ccgtccttcg ccgaggagaa gaccatccgc ttcgatccgc tctgggatcc ggctctccac
     6121 gccgcgggct tcgccggagc ggccgcgagc cgcctggcct tggagggcgc cgaccgcgcc
     6181 ctccccgacc ccgccgagct ctcgggcttc gacgcatcct tgcgcttcga ctacgacgag
     6241 gggctctcga cgctctccac cggcgcttcg ggcctcgcca tcctctggcc ggccctattc
     6301 gcgctccagg gagagaggga ggagatcttc cccgcggcgg cggcctgcgc cgaggccctg
     6361 gccatgacct acgccgcgaa gaacgcgatg aaggcgctct tccccaaggc gcggccctac
     6421 gcctatggcg ggggagagct ctccgccgag ctccgcgaag aggcctggga gtccttcccc
     6481 tcgggccacg cggccctggc cttctgcgcc gccacctcct tcgccgtcct ctccctcgag
     6541 ctcgcgccgg aaaacccggc gaccccctgg ctcgtcgcgg ggggctacgg cctcgc
//
LOCUS       DAOOSJ010000158         6595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7196_length_6595_cov_4.086850, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000158 DAOOSJ010000000
VERSION     DAOOSJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7196_length_6595_cov_4.086850"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLG14_08475"
     CDS             <1..813
                     /locus_tag="PLG14_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49204.1"
                     /translation="AMLLSLRSVVADVQGSAAGLAERSRRLSASSQDLSQGATEQAAS
                     MEEVSASLEQMGANIRQSAENAERAKTIAGRIAVDAKSGGEAVREMVGAMRTIVEKTA
                     IIEDIARQTNLLALNAAIEAARAGEAGKGFAVVASEVRKLAERSQKAAGEINEVSGVS
                     LSKAVTAGESIDKLVPAIASSSELVEEIATASAEQDSGTRQISQAVLQLDTVVQRNAS
                     SSAELVGMADELSDIAESLVSSVGYFRLSSDGEASRALAAPAGKDLPAPAED"
     gene            995..1807
                     /locus_tag="PLG14_08480"
     CDS             995..1807
                     /locus_tag="PLG14_08480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOX49205.1"
                     /translation="MPIRLIALDLDDTLLKEDLTISDANREALRAAHAAGARIVLASG
                     RNIYSMEAYARALGLAGPDDYLICSNGAEIVETATGRRLYERRMEPGLCRDVARALAE
                     RGFPWQVYEGGRILASARNPWTDEDTRLTGQPNSLVEDEEALFARGQVKYVVPGDPAK
                     IPALRDEMARLFEGRAEVLVSKPYFMEVLAAGVDKGEALSRLAGLLGLSLESTMACGD
                     AMNDLGMLRAAGLSCAPANAIPAAKEAAGFVSPLTNDEDFVADAVRRFVLKA"
     gene            2075..4459
                     /locus_tag="PLG14_08485"
     CDS             2075..4459
                     /locus_tag="PLG14_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOX49206.1"
                     /translation="MKRPALFLALTLAVQAASFAEVEFLGLDLGKDDRLLFAARTSLP
                     GDGAYDTLLSGELATGRLEQLTVYPERIALVDGGRRLQVQNRFGLFRTDASLANLAPV
                     PGYPAFARGAALPSGRILPSEPSPDGAYILQAEPSSAAYCRLVLLEAATGTTTTVAEG
                     LEYSVERFPSRWSDDSRYFVYAKRGSLYYFSVEQLAGKRVLDEDLRRIGEGRIGSVRW
                     SRDGSLYYLRGSSLYRILPAEFFTQALYRGLAGMGVLAGAIPFPYDPSFDDFWVAAEG
                     SRIILSKGGRNLFLVYLDPDDYGGERRVAALPYLYLQGGTTVRGLLWPAGGPVTVLTG
                     SIAGGERKAGAYRFEAPADPAELSLSPAVRSLEVKDVLDVVLSPDESRVALVRERGVE
                     ILGYEGWDRKAEVAAPGASNALWLDGSRLVVAGSARIEAVDLETGSRRLVALSQATAF
                     GRDAEGRILALSGGKAYARPPLGQAAAGGAAPAAAASAAASSAAVSSAAVSSAGAWTE
                     APLFAPEPAATNNEAFRVYLESSAAGPYRNAVMVRAVKTLGTRALFPLPAAAYAPFPA
                     REEPRRDGAFDHGSRIRRRELALTFDALDSGEGVVQVLNVLADYKIKATFFVNGEFVR
                     RNPGAARLLAESGHEVGSMFFTAVDPTDVRFRVDRDYIRRGLARAEDDWFAATGRELS
                     LLWHTPYYTVNSEILEAGKSMNYAYVGRDLDSLDWVSRADAARLPGSYLPAHGIIERV
                     VAAAKPGSVIPIRLGVPEGGRDDYLYSELALLVDALQCAGYEIVPVSTLMEHAE"
     gene            4486..>6595
                     /locus_tag="PLG14_08490"
     CDS             4486..>6595
                     /locus_tag="PLG14_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX49207.1"
                     /translation="MDISRLELSAAEAAFSVDFARESEAPEGAAAEAEAAAEAAAPSK
                     AGARWGSEVIGQPRALEALRMGVRIRSKGYNVYVSGAPGTGRRTAVLRVLADEPPSAE
                     RLKDIAYVYDFDAPLAPRVLLFPAGEGREFKRDLHRFVESLKRVVSLQAESEGQKRRE
                     AEATASLEAEENRRLAELEALLAADGFRAVQVEGEGGTTMDIVPLLKEGRPAGGAAAQ
                     ASAPEARQAGAADAGSASGAAAPAAADSAGAEGGTGAAPETAAGEEDVEEADGLEPSS
                     FEALQDLVASGALPEAEYNRLRELWYGHMDRMKRLFAELKKGRLELETRLTEERDEAL
                     KPQVAAEAGLLLERWQDPTVHGWIESLAKDVEERLYLFRVPDQPAEGRKRRSPALTRY
                     GINLVADRSGADRAPVVVENHPTAANLFGFVEPRPEEEGDTRGAYLRIRAGSFLQASG
                     GFLVLRAEDVVADEEAWVRLKRVLQDGRVEIQPREGPMGGAAWLKPEAVKADVKVVMI
                     GGESTYDLLYQADPDFQKLFKVHAEFDSTMPRSAEALRDYASFVRVIVEEEGLLPVSA
                     EGLAAVAEEGVRLSEYRTRLSTRFGLVADLLREADYRARLNGGTAIDSAAVRGAVAAR
                     ARLSGLPEEKIGDMIASGEIILQASGTAVGRLNGLAVHDRGYYAFGLPAVISAQVSPG
                     ESGVINIEGESGLSGEIYDKA"
BASE COUNT          915 a         2603 c         2222 g          855 t
ORIGIN      
        1 gccatgctcc tctccctcag gtcggtcgtc gccgacgtcc agggctcggc cgccggcctc
       61 gcggagagga gccgccgcct ctccgcctcc agccaggacc tctcccaggg cgcgaccgag
      121 caggccgcct ccatggagga ggtctccgcc tccctggagc agatgggggc gaacatcagg
      181 cagagcgccg agaacgccga gcgggccaag accatcgccg ggcgcatcgc cgtggacgcg
      241 aagtccggcg gcgaggccgt gcgggagatg gtcggcgcga tgcgcaccat cgtggagaag
      301 accgccatca tcgaggacat cgcccgccag accaacctcc tggccctcaa cgccgccatc
      361 gaggccgccc gcgccggcga ggccggcaag ggcttcgccg tcgtcgcgag cgaggtgagg
      421 aagctcgcgg agcggagcca gaaggccgcc ggcgagatca acgaggtctc cggcgtctcc
      481 ctgtccaagg ccgtgacggc cggggagagc atcgacaagc tcgtccccgc catcgcctcc
      541 tcctccgagc tcgtggagga gatcgccacg gccagcgccg agcaggactc cgggacgagg
      601 cagatcagcc aggccgtcct ccagctcgac acggtcgtcc agcgcaacgc ctcgagctcc
      661 gccgagctcg tcggcatggc ggacgagctc tccgacatcg cggagagcct cgtctcctcc
      721 gtgggctact tcaggctctc ctcggacggc gaggcttccc gggccctggc cgccccggcg
      781 ggcaaggacc ttcccgcccc cgccgaggac tgaccgcttt actagccgcc atcggggccg
      841 cacggcgctt gccgggcggc cctttctttt ataggcgccc ccgcggccgg cgcccggcgg
      901 acacagtgag ccaggtcggc gcggccggcg cgcgaccggc aagcgatcgg cgccgcccct
      961 cgaccagggc cgcggatcgg cgctataaac ttccatgccc atacgactca tcgccctcga
     1021 cctggacgac accctgttga aggaagacct caccatctcc gacgcgaacc gcgaggccct
     1081 ccgcgccgcc cacgccgccg gagccaggat agtcctcgcc tccggccgga acatctacag
     1141 catggaggcc tacgcccggg ccctgggcct cgcggggccg gacgactacc tcatctgctc
     1201 caacggcgcg gagatcgtgg agacggcgac cggcaggcgg ctctacgagc gcaggatgga
     1261 gccggggctc tgccgcgacg tggcgcgggc cctcgcggag cgcggcttcc cctggcaggt
     1321 ctacgagggc ggccgcatcc tcgcgagcgc ccgcaatccc tggaccgacg aggacacgcg
     1381 gctcacgggc cagcccaact ccctggtcga ggacgaggag gccctcttcg cccgcggcca
     1441 ggtcaagtac gtcgtcccgg gggatcccgc gaagatcccc gccctccgcg acgagatggc
     1501 ccgcctcttc gagggcaggg ccgaggtcct cgtctccaag ccctacttca tggaggtcct
     1561 ggccgccggg gtcgacaagg gcgaggccct ctcccggctc gccggcctcc tcggcctcag
     1621 cctcgaatcg acgatggctt gcggggacgc gatgaacgac ctgggcatgc tccgggccgc
     1681 gggcctctcc tgcgcgccgg ccaacgccat ccccgccgcc aaggaggcgg ccggcttcgt
     1741 ctcccccctc acgaacgacg aggacttcgt cgccgacgcc gtaaggcgct tcgtcctcaa
     1801 ggcctagggc cagggcggcg gcgggcggca tcccaggccg cgcgaggacg agccccacgc
     1861 cccacgcccc gcgccccacg ccccgcggcc gcccccgcgc cgcccgcccg gtcagcgccc
     1921 cggggcgact gcgcgacggc cgtttcgcgc gcgcccgccg cgccgcgccc gcgcgggcgg
     1981 cgggctaccg gcgcgcggat acgccccgcc cgcgcgatac gcgcgtttac ttctcctccc
     2041 ttttcgccga gaatgatgtg ggagggcaca gcccatgaag cgaccagcct tattcctcgc
     2101 cttgaccctc gccgtccagg cggcttcctt cgcggaggtc gagttcctcg gactcgacct
     2161 cgggaaggac gaccgcctcc tcttcgccgc ccgaacgtcc ctcccgggcg acggcgccta
     2221 cgacaccctc ctctccgggg aactcgccac cgggaggctc gagcagctca cggtctaccc
     2281 cgagcggata gccctggtgg acggcggccg ccgcctccag gtgcagaacc gcttcggcct
     2341 cttccgcacc gacgcgtcac tcgcgaacct ggcccccgtc ccgggctatc ccgccttcgc
     2401 caggggggcg gcccttccct cgggcaggat cctcccctcc gagccgagcc ccgacggcgc
     2461 ctacatcctc caggccgagc cgagctcggc cgcctactgc cgcctcgtcc tcctggaggc
     2521 cgcgaccggc acgacgacga ccgtcgccga gggcctcgag tactcggtgg agcgcttccc
     2581 ctcccgctgg tccgacgact cccgctactt cgtctacgcg aagcgcggct ccctctacta
     2641 tttctccgtc gagcagctcg cggggaagcg ggtcctggac gaggacttaa ggcggatcgg
     2701 cgaaggccgc atcggctcgg tccgctggag ccgcgacggc agcctctact acctccgcgg
     2761 ctcctccctc taccgcatcc tccccgcgga gttcttcacc caggccctct accgcggcct
     2821 cgcggggatg ggcgtcctcg cgggagccat ccccttcccc tacgacccga gcttcgacga
     2881 cttctgggtg gccgcggagg gctccaggat catcctctcc aagggcgggc ggaacctctt
     2941 cctcgtctac ctggacccgg acgactacgg cggggagcgc cgcgtcgcgg ccctccccta
     3001 cctctacctc cagggcggga cgacggtccg aggcctcctc tggccggccg gcggccccgt
     3061 caccgtgctt acgggctcga tcgcgggcgg cgagcgcaag gcgggagcct accgcttcga
     3121 ggccccggcc gatccggccg aactctcctt gagccccgcc gtccgctccc tcgaggtcaa
     3181 ggacgtcctg gacgtcgtcc tctcccccga cgagagccgc gtcgccctgg tccgggagcg
     3241 gggcgtcgag atcctgggct acgagggctg ggacaggaag gccgaggtcg cggcccccgg
     3301 cgcctcgaac gctctctggc tggacggctc ccgcctcgtc gtggcgggct ccgcccgcat
     3361 cgaggccgtc gacctcgaga ccggctcgag gcgcctcgtc gccctctccc aggccacggc
     3421 cttcggccgc gacgccgagg gcaggatcct cgccctctcg ggcggcaagg cctacgcgcg
     3481 gccgcccctc ggccaggccg cggcgggcgg ggcggctccg gcggccgcag cctcggccgc
     3541 cgcatcctcg gcagccgtat cttcggcagc cgtatcttcg gcaggggcct ggaccgaggc
     3601 ccccctcttc gcgcccgagc ccgcggcgac caacaacgag gccttccgcg tctacctgga
     3661 gagctcggcg gcaggcccct accgcaacgc ggtgatggtc cgcgcggtga agaccctcgg
     3721 cacgagggcc ctcttccccc tgccggccgc ggcctacgcg cccttccccg cgcgcgagga
     3781 gccccggagg gacggcgcct tcgaccacgg ctcccgcatc aggcgccgcg agctggccct
     3841 caccttcgac gccctggact cgggcgaagg cgtcgtccag gtcctcaacg tcctggccga
     3901 ctacaagatc aaggccacct tcttcgtcaa cggcgagttc gtcaggcgca atcccggcgc
     3961 cgcccgcctc ctcgcggaat cgggccacga ggtcggctcg atgttcttca cggccgtcga
     4021 cccgacggac gtccgcttcc gcgtcgaccg cgactacatc cgccggggcc tcgcccgcgc
     4081 ggaggacgac tggttcgccg ccacgggccg cgagctctcc cttctttggc acacgcccta
     4141 ctacacggtg aacagcgaga tcctggaggc gggcaagtcg atgaactacg cctacgtcgg
     4201 ccgggacctc gacagcctgg actgggtgag cagggccgac gcggcccgcc tccccggctc
     4261 ctacctcccc gcccacggca tcatcgagcg cgtcgtcgcc gcggccaagc cgggctccgt
     4321 catcccgata cgcctgggcg tccccgaggg cggccgcgac gactacctct acagcgagct
     4381 ggccctcctc gtcgacgccc tccagtgcgc gggctacgag atcgtgcccg tctccaccct
     4441 gatggagcac gccgaatgag gccgccagcg gccgtgggag gcgacatgga catctccagg
     4501 ctcgagctca gcgccgccga ggcggccttc tccgtcgatt tcgcccgcga gtcggaggcc
     4561 cccgaggggg ccgcggccga agcggaggcc gcggccgaag cggcggcccc gtcaaaggcc
     4621 ggggcgcgct ggggcagcga ggtcatcggc cagccccgcg ccctcgaggc cctcaggatg
     4681 ggcgtcagga tcaggtccaa gggctacaac gtctacgtct cgggggcgcc gggcacgggc
     4741 cgccggacgg ccgtcttgcg cgtcctggcc gacgagccgc cttcggccga gcggctcaag
     4801 gacatagcct acgtctacga cttcgacgcc cccctcgcgc cgcgggtcct cctcttcccc
     4861 gccggcgagg gccgcgagtt caagcgcgac ctccaccgct tcgtcgagtc cctgaagcgc
     4921 gtcgtctccc tccaggcgga gagcgaaggc cagaagcgcc gcgaggcgga ggctacggcc
     4981 tccctggagg ccgaggaaaa ccgccgcctg gccgagctcg aggccctcct cgccgcggac
     5041 ggcttccgcg cagtccaggt ggagggcgag ggcgggacca cgatggacat cgtgcccctc
     5101 ctcaaggagg gaaggcccgc cggcggggcg gccgcccagg cttccgcgcc ggaggccagg
     5161 caagccggcg ccgccgacgc cgggagcgca agcggcgccg ccgcccctgc cgccgccgac
     5221 tcggccggag ccgagggcgg gacgggcgcc gcgcccgaga ccgccgccgg cgaagaagac
     5281 gtcgaggagg ccgacggcct cgagccctcg agcttcgagg ccctccagga cctcgtcgcc
     5341 tcgggcgccc tccccgaggc cgagtacaac cgcctccgcg agctctggta cggtcacatg
     5401 gaccgcatga agcgcctctt cgccgagctc aagaagggca ggctggagct ggagaccagg
     5461 ctcacggagg agcgggacga ggccctgaag ccccaggtcg cggccgaggc cggcctcctc
     5521 ctcgagcgct ggcaggaccc gacggtccac ggctggatcg agagcctcgc gaaggacgtc
     5581 gaggagcggc tctacctttt ccgcgtcccc gaccagcccg ccgagggcag gaagaggcgc
     5641 tcgcccgccc tgacccgcta cggcatcaac ctggtcgccg accgctccgg cgccgaccgc
     5701 gcccccgtcg tcgtggagaa ccaccccacg gccgcgaacc tcttcggctt cgtcgagccg
     5761 cggccggagg aggagggcga caccagggga gcctacctgc gcatccgcgc gggctccttc
     5821 ctccaggcct cggggggctt cctcgtcctc cgcgcggagg acgtggtcgc cgacgaggag
     5881 gcctgggtcc gcctgaagcg cgtcctccag gacggccgcg tggagatcca gccccgcgag
     5941 gggccgatgg gcggggccgc ctggctcaag cccgaggcgg tgaaggccga cgtcaaggtc
     6001 gtgatgatcg gcggcgagtc gacctacgac ctcctctacc aggccgaccc cgacttccag
     6061 aagctcttca aggtccacgc cgagttcgac tccacgatgc cccggagcgc cgaggccctg
     6121 cgggactacg cctccttcgt ccgcgtcatc gtcgaggagg agggcctcct ccccgtctcg
     6181 gccgagggcc tggccgccgt cgcggaggag ggcgtgcgcc tctccgagta ccggacgcgg
     6241 ctctccaccc gcttcggcct cgtcgccgac ctcctgcgcg aggccgacta ccgggcccgc
     6301 ctgaatggcg ggacggcgat cgactcggcc gccgtccgcg gagccgtcgc cgcgcgggcc
     6361 cgcctctcgg gcctccccga ggagaagatc ggcgacatga tcgcctccgg ggagatcatc
     6421 ctccaggcca gcgggacggc ggtaggccgg ctcaacggcc tcgccgtcca cgaccggggc
     6481 tactacgcct tcggcctccc cgccgtgatc tcagcccagg tgagccccgg cgagtcgggc
     6541 gtcatcaaca tcgagggcga gtccggcctc tccggcgaga tctacgacaa ggccg
//
LOCUS       DAOOSJ010000159         6507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7316_length_6507_cov_6.377402, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000159 DAOOSJ010000000
VERSION     DAOOSJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7316_length_6507_cov_6.377402"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PLG14_08495"
     CDS             <1..1574
                     /locus_tag="PLG14_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HOX49208.1"
                     /translation="IADGLLFPDRGPKPAMAECAHLFRPVRAYASLPESLRVRAVRNS
                     SPPPASSGARLGRVFVENRFDFSDLSGVSLSWTVVSGDRSRPERDGIVARGGAALPPS
                     SPGGVEAVELGLPLSGEAGEALRSAMRDGECVLELEFSLSRTLPWAEAGHVLAREQLS
                     LSEAPLAARRPVRGKGASARASGAVASGAVASGSGGSAPGPAGGPAGGQAGGEGLLEA
                     RFSDEGFLASLKGPSGAELLASPLLPCLFRAPTQNDGLKNFMSLRGQSDFAFYYTDKA
                     MYGWLDAGLDRLVCSLESREESGGVLRLVHGLSTAKGAKTGRLYQSFRIEGRRVEADF
                     VFDLDPGLPELPRVGLRGRLAPGLENARWFGLGPHEAYSDRRAGARLGVWSAYAAGLA
                     VPYIVPQENGNRHGTRWLELYSASDAAPSSDAATRGVAPLAAAAASRGAPALAVEGVR
                     PFDFSLSPYSDEELWEGRHWDGLRPFREALKEGAWLHLDAAQRGVGTATCGPDTLERH
                     RLRPGVYRLSLAFEA"
     gene            complement(1741..3096)
                     /locus_tag="PLG14_08500"
     CDS             complement(1741..3096)
                     /locus_tag="PLG14_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49209.1"
                     /translation="MSKATRPRSIFLGLSSFQALAMFRRGIFYTFLGVYLRGYLGLSV
                     TETTLFETIPMVLNILFQSFVWGRLADRRQLRRSLIVAGELVAAVGHPLMWLAHAACP
                     DPRAAGWAVIWGLSLIEIFWAMSNIGWSAYLSDVYAPGERAAVQGRLASVGGAGRILG
                     AAAGGLLYDGAGRAFAGWGYAKGGIFLVASGAMLVSVIPLLFMPEGGVGYRPEEAARD
                     ADAPPASGRAPAGTSARGAGGRSSAGSGTGGDARRFAVFLAGMLLVNCGVNSLGAFRA
                     QYLNLAGGFAASPGEVSLAANAESAALILVGLAMGALGRRLGAARLLVLGTAAGIAAV
                     LGYALAPSLGLVYAAALLKGLSDGAVAAASYAYASTLIPPERRGRYFAAYNATFQLSW
                     GLSATLVTGPLIDALVAAGRPESFAYRAGFLSAAAIMILGLGLLAPLLARPSPGRRPG
                     R"
     gene            complement(3204..4085)
                     /locus_tag="PLG14_08505"
     CDS             complement(3204..4085)
                     /locus_tag="PLG14_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOX49210.1"
                     /translation="MVDYFGQLRRAVLFIEERLDEDIGFREVAAEAAYVSPWHFHRIF
                     EAVLGLTVTEYIRERRLSVAALRLVETEDRVVDIALDALYDSQEAFTRAFKRRFGLPP
                     GRYRERGVKASLPSREPYSAMALRAMAGGIDMEPKIERKEAFTLVGIAGKTSQADNRI
                     PALWAAFLPRAGEVPRRRSGGGSYGVCEYVDPLSMSEDTEWTEFVGVEVEEDAAAEPP
                     EGMVRKALPSRRYAVFTHKGGLEALGATYDYIYKTYIPRSGLALAEAEDFEYYDGRFD
                     PENPSASEMYIYIPLAD"
     gene            4306..5772
                     /locus_tag="PLG14_08510"
     CDS             4306..5772
                     /locus_tag="PLG14_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX49211.1"
                     /translation="MKQNPSLALRAVLAVLLETGFYALSIGLAALLAAVPVLELLVAE
                     RLHLKLALFCLVGAGTIVWSIFPRRDRFSAPGPEISREEAPGLHALVDELAAATGQPA
                     PERIYLVPDANAWVARRGGFMGIGARPLMGIGIPFVAGLGAGELRAVLAHEFGHFVGG
                     DVRLGPRIWRTRAAIERSVESLGKRSGLLQAPFLAYGRLYLRITQAVSRAQEFAADAL
                     SARLAGPAAAAGALRSTTRISAAHELYWGNELGPVLSRGFAPPLAEGFRRYLEAPAIA
                     PALAAAEARELESDATGAYDSHPSTRARLDAIAALGLPAPAGGEDGPAASLVPGLPAL
                     EAALLARLAGEGRKFEPLAWESTGEAVWLAYWREWAKELPAPPAGTPLAEAARLAADA
                     ALFPAEAEPTREGETPAEAARSRKVSSLGFNLALALRAAGWTAATGPGEQLRLVSGDK
                     AFDPFEAAAALASGELGDEAWRGRCAALGFAEVPFTAA"
     gene            complement(6062..>6507)
                     /locus_tag="PLG14_08515"
     CDS             complement(6062..>6507)
                     /locus_tag="PLG14_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOX49212.1"
                     /translation="PKELWPNEKLTAEMKAVAQETGGKIEFYSIENADQALKGADALY
                     TDVWVSMGEESKFEERIKQLEKYRVTMDFMKKTGNPDCLFLHCLPAFHDTETDVGKDI
                     EKKYGLKSMEVADDVFRCRTSKVFDEAENRLHTIKAVMVATIGDL"
BASE COUNT          801 a         2440 c         2372 g          894 t
ORIGIN      
        1 tcatcgccga cggcctcctc ttccccgacc gcggccccaa gcccgcgatg gccgaatgcg
       61 cccacctctt ccgtccggtc cgcgcctacg cctccctgcc ggagtccctg cgcgtccgcg
      121 ccgtccggaa ctcgagtccg ccgcccgcct cctccggcgc gcgactgggg cgcgtcttcg
      181 tggagaaccg cttcgacttc tcggacctct ccggcgtctc cctttcctgg accgtcgtct
      241 cgggcgaccg ctcccgcccg gagcgcgacg ggatcgtcgc ccgcggcggc gcggccctgc
      301 cgccgagttc ccctggcggc gtcgaggccg tggagctcgg cctcccgctc tccggcgagg
      361 cgggggaggc cctcaggagc gcgatgcgcg acggcgagtg cgtcctcgag ctcgagttct
      421 cgctctcccg cacgcttccc tgggccgagg ccggccacgt cctcgcccgc gagcagctct
      481 ccctgtcgga ggcgcccctc gcggcccgcc gcccggtgcg ggggaaaggc gcttcggcgc
      541 gcgcgagcgg cgctgtcgcg agcggcgctg tcgcgagcgg gagcggcggc tccgcgcccg
      601 gcccggctgg cggcccggct ggcggccagg ccggcgggga aggcctccta gaggcgcgct
      661 tctctgatga gggcttcctc gcctcgctca aggggccttc gggcgcggag ctcctcgcct
      721 cgcccctcct tccctgcctc ttccgcgcgc ccacccagaa cgacgggctc aagaacttca
      781 tgtcgctccg cggccagagc gacttcgcct tctactacac cgacaaggcg atgtacggct
      841 ggctcgacgc gggcctggac aggctcgtct gctccctcga gtcgcgggag gagtcgggcg
      901 gcgtccttcg cctcgtccac ggcctctcca cggccaaggg cgcgaaaacc ggacgcctct
      961 accagtcctt caggatcgag ggccgcaggg tcgaggcgga tttcgtcttc gacctggatc
     1021 ccggcctgcc cgagctgccc cgcgtcggcc tgcgcggccg cctggcccct ggcctggaaa
     1081 acgcgcgttg gttcggcctc ggcccccacg aggcctactc ggaccgccgc gccggggcgc
     1141 gcctgggcgt ctggtcggcc tacgcggcgg gcctcgccgt cccctacatc gtcccccagg
     1201 agaacggcaa ccggcacggg acgcgctggc tcgagctgta ctcggcctcg gacgcggcgc
     1261 cctcctcgga cgcggcgacg cgcggcgtgg ctccccttgc cgcggccgcg gcgtcgcgcg
     1321 gcgcgccggc cctcgcggtc gagggcgtca ggcccttcga cttctccctc tcgccctact
     1381 cggacgagga gctctgggaa ggccgacatt gggacggcct tcgccccttc cgcgaagcgc
     1441 tcaaggaggg agcctggctc cacctggacg cggcccagcg cggcgtgggc accgcgacct
     1501 gcgggcctga cacgctggag cgccaccgcc tgaggccggg agtctaccgg ctgtccctcg
     1561 ccttcgaggc ctgatcgaag ggccgggcgc gcgggtggcg ccgcccgcgg cggcatgggc
     1621 ggggcgcgag ccggccgtcg acgcggccgc gctcgggcct acccgcgccg ctcagcctcg
     1681 cggccctccg cgaagccggt gcgcctgccg cagcctgcct cggcctgtcg cggcctgcgc
     1741 ctagcggccg ggccgcctgc cgggacttgg ccgcgcaagg agaggcgcga ggaggccgag
     1801 gccgaggatc atgatcgcgg ccgcggagag gaagcccgcg cggtaggcga aggactccgg
     1861 ccttcccgcg gcgacgaggg cgtcgatgag ggggcctgtc acgagggtcg cggagagccc
     1921 ccaggagagc tggaaggtcg cgttgtaggc ggcgaaatag cgaccccgcc tttcgggcgg
     1981 gatcagcgtg gaggcgtagg cgtaggaggc cgcggcgacc gcgccgtcgg agaggccctt
     2041 gaggagggcc gccgcgtaga cgaggcccag gctcggcgcg agggcgtagc ccaggaccgc
     2101 cgcgatcccg gccgcggtgc ccaggacgag gagccgggcc gcgccgagcc tccgcccgag
     2161 agcgcccatc gcgaggccca cgagaatcag ggccgccgac tccgcgttcg cggccaggct
     2221 cacctcgccg ggggaagcgg cgaagccccc cgccaggttc aggtactgcg ccctgaaggc
     2281 tccgagcgag ttgacgccgc agttcacgag gagcatcccg gccaggaaga ccgcgaaacg
     2341 ccgcgcgtcg ccgccggtgc cggaccccgc ggagcttcgg cctccggctc cgcgcgcgga
     2401 ggtcccagcc ggagcgcgtc ccgacgccgg gggagcgtcc gcgtcgcgag cggcctcctc
     2461 ggggcgatag ccgacgccgc cttcgggcat gaagaggagg gggatgacgg agacgagcat
     2521 cgcccccgac gcgacgagga agatgccgcc cttggcgtag ccccagccgg cgaaggccct
     2581 gcccgcgccg tcgtagagga ggccgcccgc ggccgccccg aggatgcggc ccgccccgcc
     2641 gacgctcgcg aggcggccct gcacggcggc gcgctccccg ggggcgtaga cgtcggagag
     2701 gtaggcgctc cagccgatgt tcgacatggc ccagaagatc tcgatgaggg agagtcccca
     2761 gatgacggcc cagcccgcgg cgcgggggtc ggggcaggcc gcgtgggcga gccacatgag
     2821 gggatggccg accgccgcga cgagctcgcc cgcgacgatg aggctccgcc tgagctggcg
     2881 gcggtcggcg agcctccccc agacgaagga ctggaaaagg atgttgagga ccatcgggat
     2941 cgtctcgaag agggtcgtct ccgtgacgga gagcccgagg tagccgcgga ggtagacgcc
     3001 gaggaaggtg tagaagatgc cccgcctaaa catcgcgagg gcctggaagg aggagaggcc
     3061 gaggaatatg gagcgggggc gcgtggcttt gctcaaggcg ccgccatagt gagggaagcg
     3121 ccgcgcgcgg gtcaagggcc gcggcggagg atcgcttcgg cccgccgaac ggcgccgtgg
     3181 cccgccgccg ccccgcgccg cggctagtct gccaggggaa tgtagatgta catctccgag
     3241 gcggagggat tctccggatc gaagcggccg tcgtagtatt cgaagtcctc cgcctccgcc
     3301 agggcgaggc cggagcgcgg gatgtaggtc ttgtagatgt agtcgtaggt cgcgccgagg
     3361 gcctcgaggc ctcccttgtg cgtgaacacg gcgtagcggc gggaaggcag ggccttgcgg
     3421 accatgccct caggcggctc cgccgcggcg tcctcctcga cctcgacgcc cacgaactcc
     3481 gtccactccg tgtcctcgct catcgagagg ggatcgacgt actcgcagac gccgtagctt
     3541 ccgccgccgc tcctcctccg cggcacttcc cccgcgcgcg gcaggaaggc cgcccatagg
     3601 gcgggtatgc ggttgtcggc ctgcgaggtc ttccccgcga tgcccaccag cgtgaaggct
     3661 tccttgcgct cgatcttcgg ctccatgtcg atgcctcctg ccatcgcgcg gagggccatg
     3721 gccgaatagg gttccctaga cggcagggag gccttcacgc ctcgttcgcg gtagcgcccc
     3781 ggcggcaggc cgaagcggcg cttgaaggcc ctggtgaagg cctcctgcga atcgtagagc
     3841 gcgtccaagg cgatgtcgac gacgcggtct tccgtctcga cgagtcggag ggccgcgacg
     3901 ctcaggcgcc gctcgcgtat gtactcggtg accgtgaggc ccaggaccgc ctcgaagatc
     3961 cggtggaagt gccagggcga gacgtaggcc gcctccgccg cgacctcgcg gaatccgatg
     4021 tcctcgtcca ggcgctcctc gatgaagagc acggcgcggc gcagctgccc gaagtagtcg
     4081 accatggtcc gcgcctccct cgcgcgatgc gggcctatcc tagccgcctg gtcccgggtc
     4141 ggtcttgatc cggattgcgg aagcgcaccg cgccggcgtc ctgggccgct ccgcgggcgc
     4201 ggggggcgcg ctttcgtcca tcggcagccc gcccccgccc ggaacggctt gacttggaat
     4261 gcgggccggt gcaggattcg cgcatcccgc cgcgcggagt ctagcatgaa acagaaccct
     4321 tcgctcgccc tcagggccgt cctcgccgtc ctcctcgaga ccggcttcta cgccctctcg
     4381 atcggcctcg cggccctcct cgccgcggtg cccgtcctcg agctcctcgt cgcggagcgg
     4441 ctccacctga agctcgccct cttctgcctc gtcggcgcgg gcacgatcgt ctggtccatc
     4501 ttcccccgcc gcgaccgctt ctcggcgccg ggacccgaga tctccaggga ggaggcgccc
     4561 ggcctccacg ccctcgtgga cgagctcgcg gccgcgacgg gccagcccgc tccggagcgg
     4621 atctacctcg ttccggacgc gaacgcctgg gtcgcccgcc gcggcggctt catggggata
     4681 ggggcgcggc cgctcatggg cataggcatt cccttcgtcg cgggccttgg cgcgggcgag
     4741 ctccgcgcgg tgctcgcgca cgagttcggc cacttcgtcg gcggcgacgt caggctcggc
     4801 ccccggatct ggcgcacgcg ggcggccatc gagcgctcgg tggagagcct cggcaagcgc
     4861 tcggggctcc tccaggcccc cttcctcgcc tacggccgcc tctacctccg gatcacccag
     4921 gccgtctccc gcgcccagga gttcgcggcc gacgccctct ccgcgcggct cgcggggccg
     4981 gcggccgcgg cgggcgccct gaggtcgacg acgcggatct ccgccgccca cgagctctac
     5041 tggggcaacg agctcgggcc ggtcctctcc cggggcttcg ctccgccgct cgcggagggc
     5101 ttccgccgct acctcgaggc gccggccatc gccccggccc tcgcggccgc cgaggcccgg
     5161 gagctcgagt cggacgcgac gggcgcctac gactcccacc cctcgacccg ggcgcgcctc
     5221 gacgcgatag ccgcccttgg cctgccggcc cccgcgggcg gggaggacgg ccccgcggcc
     5281 tccctcgtcc ccgggcttcc cgccctggag gcggctcttc tcgcgcgcct cgcgggcgag
     5341 gggcggaagt tcgagccgct ggcctgggag tcgacgggcg aggccgtgtg gctcgcctat
     5401 tggcgcgagt gggcaaagga gctgccggcg ccgcccgcgg ggacgcccct cgccgaggcc
     5461 gcccgcctcg ccgcggacgc cgccctcttc cccgcggagg ccgaacccac ccgcgagggc
     5521 gagacgcccg cggaggccgc gcgctcccgc aaggtctcct ccctcggctt caacctggcc
     5581 ctcgcccttc gggcggcggg ctggacggcc gcgacggggc cgggcgagca gctcaggctc
     5641 gtctccggag acaaggcctt cgaccccttc gaggccgccg cggccctcgc ctcgggcgag
     5701 ctcggagacg aggcctggcg cgggcgctgc gcggccctcg gcttcgcgga ggtccccttc
     5761 acggccgcct gacgcgccgc ggccagaggc gcccgcggcg cggcttcaat gacgagaggg
     5821 ggcagggcgg gatcgcccga gccgcccgcg cggcgcttca ggcggcgggc tcgtccgaga
     5881 ccgccgctcc cggcgcggcc cgcggcgagg gaagcccgcc cgaggggggg ccgcgtcatg
     5941 cgatcccgct ccgggggcga aggcggccgc gcggaggccc ggcgcccccc cctggcccgc
     6001 gcgggaagaa aaaacgcccg gcctcgcggc cgggcgtccg tccaggcggc gaaggcccgg
     6061 cttacaggtc cccgatcgtc gcgaccatga cggccttgat cgtgtggagg cggttctccg
     6121 cctcgtcgaa gaccttggag gtgcggcagc ggaagacgtc gtcggcgacc tccatggact
     6181 tgaggccgta cttcttctcg atgtccttgc ccacgtccgt ctccgtgtcg tggaaggcgg
     6241 ggaggcagtg gaggaagagg cagtcggggt tgccggtctt cttcatgaag tccatcgtga
     6301 cgcggtactt ctcgagctgc ttgatgcgct cctcgaactt ggactcctcg cccatggaga
     6361 cccacacgtc ggtgtagagg gcgtcggcgc ccttcagggc ctggtcggcg ttctcgatgg
     6421 agtagaactc gatcttgccg ccggtctctt gcgcgacggc cttcatctcc gcggtgagct
     6481 tctcgttcgg ccagagctcc ttggggg
//
LOCUS       DAOOSJ010000160         6492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7347_length_6492_cov_4.838123, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000160 DAOOSJ010000000
VERSION     DAOOSJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7347_length_6492_cov_4.838123"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLG14_08520"
     CDS             <1..1102
                     /locus_tag="PLG14_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOX49213.1"
                     /translation="GGSPMTKTARILVGPAPHIHDSQSTASLVWGCVLALVPALLWGV
                     YCFGVGAALVVAAALGGALAGEGLAGLARRRFSLLDGSAFLTGLLVGLAMPPGAPLYV
                     PAASAFFATAFVKGAFGGLGSNWMNPALAGIAFAVLNWPQAMGAWVLPRHLTGVVGIS
                     GATPLALVRDKLASGQGGSDPLAILEAAGQSFTGLDRGVTGFLNGGLFERLGASLPQG
                     YIDLLVGNRPGAVGELSGLLLLAASVVLVARRMIRWEVPLSIFASYAALVWAFGGLPF
                     GEGFFSGDVLFEVLSGSFILVSFFMATDPVTSPSTRLGMAVYGAGVGALTFLLRSFGS
                     RPEGTAFAVILMNCALPLLERLERPRRGEAAK"
     gene            1099..1662
                     /locus_tag="PLG14_08525"
     CDS             1099..1662
                     /locus_tag="PLG14_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49214.1"
                     /translation="MSADKSNKGIPAKKETLAENAVAAAFLGLLAVLVAAAGLLVGSG
                     AEREERKALAELSGRRVAPLATAVGGQGFERVYGLEGKGARRYGAVALVGTPRGSALA
                     AFVLAADGELEAARLLGEGTAGQPYSREGWFSEFLGRGAERAYPRTKAASRSPEALSG
                     ATESFAATADLLESLSAAVRRVAEKKE"
     gene            1665..2273
                     /locus_tag="PLG14_08530"
     CDS             1665..2273
                     /locus_tag="PLG14_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOX49215.1"
                     /translation="MEARREPFTPDPLLGALIGLCPVVAASDSLADGVILGLGAALSA
                     LVLGLSLPSLKSLMPARLRSPMAFALAAGLAALYGLAAEAYSPLLAKGLGIYLPLVAV
                     NCLVLASLRRGLRPGEDSALGLAATVFLYFLVSVLVAGLREVLGAGRLTLPTPGLSPR
                     SLLILESPPLRLLVAPAGGFMVLGCLAAAYRLVQRVRGRRLP"
     gene            2270..2854
                     /locus_tag="PLG14_08535"
     CDS             2270..2854
                     /locus_tag="PLG14_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOX49216.1"
                     /translation="MSYLGIVLVSAFASNVLLAYGLGVCPAFRRDRESPLVSILALGL
                     VNTLAAAALWILRVLVLEPLRLGSLDVLLFAVLVAPALKYLARAVASLESPLLRKIGS
                     AADEAVVGCLVFGIALLSSRSGYLLPEAVLASASSCLGYWAALVLLEAVRERLELSDL
                     PASFRGAPSVLASAGLMAMAFMGIDLTLVKNLVG"
     gene            2859..3713
                     /locus_tag="PLG14_08540"
     CDS             2859..3713
                     /locus_tag="PLG14_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOX49217.1"
                     /translation="MEWLRIPFAFLIVLAASFGALFLSAAWERRRRRDPVLGALLALL
                     PGKDCGLCGADDCRHYAKRLLEEGADPSLCAPGGLPVEESLRKSLGGERGTAKFAFVR
                     CGGSKSQAADLFAYEGREDCGAAKRLFGGQKRCAEACLGLGSCARACPLGAITVREGL
                     ASVDPGRCSGCGACVAACPQGLIALAPQSARWYVACASKRPPEERKADCKVACVACGD
                     CQKLSANWEFSLEGGLARASTTVPEKGAGAGAFEGIAARCPTRAILKAGAARGKAETG
                     PAEAAKKP"
     gene            3780..5720
                     /locus_tag="PLG14_08545"
     CDS             3780..5720
                     /locus_tag="PLG14_08545"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOX49218.1"
                     /translation="MQKSHVIIGAYNHVPEGAEESFFEETYQICWRPFLSSLYRFPEV
                     VAALHYSGSVLRWLEARHPEFLMLLEEMAARKQIELLGGGFFAPLLPLVAGSDRLGQI
                     ELLTTYIRKAFGRRPRGSWIQEYAWEPGLASALQTCGFDYTFLPERHFRLAGAGLGLP
                     SVTEDQGRSVTVFPVFDALESFESLLPPDQAIERVREREGELPLVTVFYPDRAARGLW
                     EASGLESPDVLFERSFAAIQREGLPYETTTPGRFLKTSRRLARAYFPGCASELLMRRS
                     LPDLAAACAGEARGLEPSSDSGSPRRLLLRYEESMDLYAKMQYVRLLVGQLRGDKSRK
                     KTAQEELWKGQCGEAYWHGPSGGLLRLPVRASAYAALIEAEKTTRQRGSFSPGVIFAD
                     IDFDGEKEILYQGGDYNAYVHLRGGLLFELDSLKTRRNYANVLADGGCDGKPPRARRC
                     FHDRVCAQGGFEGQAGGFLDEHYSLAGSEKPAHLAVLVCEGKALLGGRERSLVVRKAF
                     AFKKGSLSLEYEISNPEAEPLAIRFATELNLAAGTDASRVGLSGDSGAGQEPVAFESG
                     IKGKGSGLKSLRLENLDDEEKLELRCERPFEFVHEPVYRSVDVDGVAHAMYQGSSVSL
                     GWDLDIPASSSTRLAVVLGLKA"
     gene            5793..>6492
                     /locus_tag="PLG14_08550"
     CDS             5793..>6492
                     /locus_tag="PLG14_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOX49219.1"
                     /translation="MSETRLPASLRGFRIHLVGAKGTGMAALAEILAAGGASLTGSDV
                     ADHFYTDEILADIGVAMAEGFDPAHLGAGVDLVIHSAAYARDRNPELLEAARRGIPIL
                     VYPEALGELSRRFDSSGIAGVHGKTTTTALAGSLVAALGLPATVLAGSAVSSFGGRST
                     KILGDRYFVAETCEYRRHFLHFSPRRVILTSVEPDHQDYYPAYGDILAAFVEYGRLLP
                     PGGELIYCADDPGAV"
BASE COUNT          792 a         2561 c         2113 g         1026 t
ORIGIN      
        1 tggaggctcg ccgatgacca agaccgcccg catcctcgtg ggccccgcgc cgcatatcca
       61 cgacagccag tccacggcct cgctcgtctg gggctgcgtc ctcgccctcg tcccggccct
      121 cctttggggc gtctactgct tcggcgtcgg cgccgcgctg gtcgtggccg cggccctggg
      181 cggcgccctg gcgggggagg ggctcgccgg cctcgcgcgg aggcgcttca gcctcctgga
      241 cggcagcgcc ttcctcacgg gccttctcgt gggcctcgcc atgcccccgg gggcgccgct
      301 ctacgtgccc gccgcctcgg ccttcttcgc cacggccttc gtgaaggggg ccttcggcgg
      361 cctcggctcg aactggatga atcccgccct cgcgggcata gccttcgccg tcctcaactg
      421 gccccaggcc atgggagcct gggtccttcc ccgccacctg accggcgtcg tcggcatctc
      481 cggcgcgacg cccctggccc tcgtccgcga caagctcgcc tcgggccagg gcgggagcga
      541 tcccctggcc atcctggagg cggccggcca gagcttcacc ggcctggacc ggggggtcac
      601 gggcttcctg aacggcggcc tcttcgagag gctgggcgcc tccctgcccc agggctacat
      661 cgacctccta gtcggcaaca ggcccggagc cgtcggcgag ctctccggcc tcctcctcct
      721 ggccgcctcg gtggtcctgg tcgcgcggcg gatgatccgc tgggaggtgc ccctctccat
      781 cttcgcgagc tacgcggccc tggtctgggc cttcggaggc ctgcccttcg gcgagggctt
      841 cttctcgggc gacgtcctct tcgaggtgct ctcgggctcc ttcatcctcg tctccttctt
      901 catggccacc gatcccgtaa cctcgccctc cacgaggctc ggcatggccg tctacggcgc
      961 cggcgtcggc gccctgacct tcctcctgcg gagcttcggc tcccgccccg aaggcacggc
     1021 cttcgccgtc atcctcatga actgcgcctt gcccctcctg gaaaggctgg agcggcccag
     1081 gcgcggggag gcggcgaagt gagcgcggac aagtccaaca aagggattcc ggccaagaaa
     1141 gagacactgg ccgagaacgc ggtcgcggcc gccttcctgg gtctcctcgc cgtcctggtc
     1201 gcggccgccg gactcctcgt cggctccggc gcggagaggg aggagcgcaa ggccctggcc
     1261 gagctctccg gccgcagggt agcccctcta gcgacagccg tcggcggcca gggcttcgag
     1321 cgcgtctacg gcctcgaggg caagggcgct aggcgctacg gggccgtggc cctggtcggg
     1381 accccccgcg gctccgccct ggcggccttc gtcctggccg ccgacggcga gctcgaggcc
     1441 gcgaggctcc tgggcgaggg gacggccggc cagccctatt cgcgcgaggg ctggttctcc
     1501 gagttcctgg gccggggcgc cgagcgcgcc tatccccgga ccaaggccgc ctcccgttcc
     1561 cccgaggccc tgagcggcgc gacggagagc ttcgcggcga ccgccgatct cctagagagt
     1621 ctgtccgccg ccgtgcgcag ggtcgcggag aaaaaggaat gagcatggaa gcgcgccgcg
     1681 agcccttcac ccccgacccc ctcctcggag ccctcatcgg cctctgtccc gtggtcgcgg
     1741 cctcggacag cctggccgac ggcgtcatcc tgggcctcgg cgcggccctc tcggccctgg
     1801 tcctgggcct ctccctgccg agcctcaaga gcctcatgcc cgccaggctc aggtcgccca
     1861 tggccttcgc cctcgcggca ggcctcgcgg ccctctacgg cctcgcggcc gaggcctatt
     1921 cgcccctcct cgcgaagggc ctcgggatct acctccccct cgtcgcggtc aactgcctgg
     1981 tcctggcctc gctcaggcgc ggcctccggc cgggcgagga cagcgccctc ggcctcgcgg
     2041 ccacggtctt cctctacttc ctcgtgtccg tcctcgtcgc cggcctgagg gaggtcctgg
     2101 gcgcaggcag gctcaccctg cccacgccgg ggctttcgcc ccgctccctc ctcatcctcg
     2161 agtccccgcc cctgcgcctc ctcgtcgcgc cggccggcgg cttcatggtc ctcggctgcc
     2221 tcgcggccgc ctatcgcctc gtccagaggg tccgcggaag gaggctcccg tgagctatct
     2281 cggcatcgtc ctcgtctcgg ccttcgcctc caacgtcctc ctcgcctatg gcctgggcgt
     2341 ctgccccgcc ttccgcaggg accgcgagag ccccctcgtc tccatcctgg ccctcggcct
     2401 cgtcaacacg ctcgcggcgg cggcgctctg gatcctcagg gtcctcgtcc tcgagcccct
     2461 gaggctcggc tccctcgacg tcctcctctt cgcggtcctc gtcgcccccg ccctcaagta
     2521 cctggcccgg gccgtggcga gcctggagag ccctctctta aggaagatcg gctccgccgc
     2581 cgacgaggcc gtcgtgggct gcctcgtctt cgggatagcc ctcctctcct cgcggtcggg
     2641 ctacctcctc cccgaggccg tcctcgcgag cgcgagctcc tgcctcggct actgggccgc
     2701 cctcgtcctc cttgaggcgg tcagggagag gctcgagctc tcggacctcc ccgcctcctt
     2761 ccgcggcgcc ccttccgtcc tcgcctccgc gggcctcatg gcgatggcct tcatgggcat
     2821 cgacctcacc ctcgtcaaga acctcgtggg ctagcggcat ggagtggctc aggattccct
     2881 tcgccttcct catcgtcctc gccgcctcct tcggcgccct cttcctctcc gcggcctggg
     2941 agaggcggag gcgccgcgat cccgtcctgg gcgccctcct ggccctcctg cccggcaagg
     3001 actgcggcct ctgcggcgcc gacgactgcc gacactacgc gaagcgcctc ctggaggagg
     3061 gcgccgaccc ctccctctgc gcgccgggcg gcctccccgt ggaggagtcc ctcaggaaga
     3121 gcctgggcgg ggagcggggg acggccaagt tcgcctttgt ccgctgcgga ggctcgaagt
     3181 cccaggccgc cgacctcttc gcctacgagg gccgtgagga ctgcggcgcg gccaagcggc
     3241 ttttcggcgg ccagaagcgc tgcgccgaag cctgcctggg ccttggctcc tgcgcgaggg
     3301 cctgccccct gggcgcgatc acggtcaggg agggcctcgc ctccgtcgat cccggacgct
     3361 gctccggctg cggcgcctgc gtcgcggcct gtccgcaggg gctcatcgcc cttgctcccc
     3421 aatcggcccg ctggtacgtc gcctgcgcct cgaagcgccc gccggaggag aggaaggccg
     3481 actgcaaggt cgcctgcgtg gcctgcggcg actgccagaa gctctccgcg aactgggaat
     3541 tctccctgga gggagggctc gcccgcgcct cgacgacggt gcccgagaag ggagccgggg
     3601 cgggggcctt cgagggcata gccgcgcgct gcccgacccg ggccatactg aaggccgggg
     3661 ccgcccgggg caaggccgaa accggccccg ccgaggcggc gaaaaaacct tgagcgccct
     3721 tccttcgccc cgcgccccgc gctataatcg cgccatacta ggctaaggac agtcggccca
     3781 tgcagaaaag ccatgtcatc atcggcgcat acaaccacgt ccccgaaggc gccgaagagt
     3841 ccttcttcga agagacctat caaatctgct ggcggccctt cctctcctcc ctgtaccgct
     3901 ttcccgaggt cgtcgcggcc ctccattatt ccggctccgt cctccgctgg ctcgaggcca
     3961 ggcaccccga gttcctcatg ctcctggagg agatggcggc caggaagcag atcgagctcc
     4021 tgggaggcgg cttcttcgcc ccgctcttgc ccctcgtcgc gggctcggac cgcctgggcc
     4081 agatcgagct cctcacgacc tacatccgca aggccttcgg ccgccggccc aggggctcct
     4141 ggatccagga atacgcctgg gagccgggac tggcctcggc cctgcagacc tgcggcttcg
     4201 actacacctt ccttcccgag cggcatttcc gcctggccgg ggcgggcctg ggccttccct
     4261 cggtcaccga ggaccagggc cgcagcgtca ccgtcttccc ggtattcgac gcgctggaga
     4321 gcttcgagag cctcctcccc cccgaccagg cgatagaacg ggtcagggag agggaagggg
     4381 agctccccct cgtcaccgtc ttctatcccg accgggccgc gcgcggactc tgggaggcct
     4441 cgggcctcga gtcgcccgac gtcctcttcg agcgctcctt cgcggccatc cagcgcgagg
     4501 gccttcccta cgagacgacg acgccgggcc gcttcctcaa gacctcccgc cgcctcgcgc
     4561 gcgcctactt ccccggctgc gcctccgagc tcctcatgag gcggagcctc cccgacctcg
     4621 cggcggcctg cgccggcgaa gcccggggcc tggagccctc ctccgactcg gggagcccgc
     4681 ggcgcctcct cctccgctac gaggagagca tggacctcta cgcgaagatg cagtacgtcc
     4741 gcctcctcgt gggccagctc cgcggcgaca agtcgaggaa gaagacggcc caggaggagc
     4801 tctggaaggg ccagtgcggc gaggcctact ggcacgggcc ctcgggcggc ctcctccgcc
     4861 tgcccgtgcg cgcctccgcc tacgcggccc tcatcgaggc cgagaagacg acgaggcagc
     4921 gcggctcctt ctcgcccggc gtcatcttcg ccgacatcga cttcgacggc gagaaggaga
     4981 tcctctacca gggcggcgac tacaacgcct acgtccacct gcgcggcggc ctcctcttcg
     5041 agctcgactc cctgaagacg cgccggaact acgccaacgt cctggccgac ggcggctgcg
     5101 acgggaagcc gccccgcgcc aggaggtgct tccacgaccg cgtctgcgcc cagggcggct
     5161 tcgagggcca ggcgggcggc ttcctggacg agcactactc gctcgcgggc tcggagaagc
     5221 cggcccacct ggccgtcctc gtctgcgagg gcaaggccct cctcggaggc cgggagcgca
     5281 gcctcgtcgt ccgcaaggcc ttcgccttca agaagggctc cctctccctc gagtacgaga
     5341 tctccaatcc cgaggccgag ccgctcgcga tccgcttcgc cacggagctg aacctggccg
     5401 cgggcaccga cgcctcgcgc gtcggcctct cgggcgactc gggggccggc caggagccgg
     5461 tcgccttcga gagcggcatc aagggcaagg gctcgggcct caagtccctg aggctcgaga
     5521 acctggacga cgaggagaag ctcgagctca ggtgcgagcg ccccttcgag ttcgtccacg
     5581 agcccgtgta ccggagcgtc gacgtggacg gggtggccca cgcgatgtac cagggctcga
     5641 gcgtgtccct gggctgggac ctcgacatcc ccgcctcctc gtcgaccagg ctcgccgtcg
     5701 tcctgggcct caaggcctag gcggccgcgg cggggcgcga tgggacacgc ggagccttgc
     5761 ccgcgcccgg gccttttcgg tagaatccag ccatgagcga aaccaggctt cccgcctccc
     5821 tgcggggctt caggatacat ctcgtcggcg ccaagggcac gggcatggcg gccctggccg
     5881 agatcctcgc cgcgggcggg gcgtcgctca cgggctccga cgtggccgac catttctaca
     5941 ccgacgagat cctggccgac atcggcgtcg cgatggccga gggcttcgac ccggcccacc
     6001 tgggcgcggg cgtcgacctc gtgatccatt ccgcggccta cgcccgcgac cgcaatcccg
     6061 agctcctgga ggcggcgagg cgcggcatcc ccatcctcgt ctatcccgag gccctgggcg
     6121 agctctcccg ccgcttcgac tcctccggca tcgcgggcgt ccacgggaag accacgacca
     6181 cggccctggc cggcagcctg gtcgcggccc tcggccttcc cgccacggtc ctcgcgggct
     6241 cggcggtctc ctccttcggc ggccgctcca cgaagatcct gggcgaccgc tacttcgtcg
     6301 ccgagacctg cgagtaccgc cgccacttcc tccacttcag cccccggcgc gtcatcctca
     6361 cgagcgtcga gcccgaccat caggactact acccggccta cggcgacatc ctcgcggcct
     6421 tcgtcgagta cggccgcctc ctccccccgg gcggcgagct catctactgc gccgacgacc
     6481 cgggagccgt gg
//
LOCUS       DAOOSJ010000161         6473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7375_length_6473_cov_6.113898, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000161 DAOOSJ010000000
VERSION     DAOOSJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_6473_cov_6.113898"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLG14_08555"
     CDS             <1..555
                     /locus_tag="PLG14_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49220.1"
                     /translation="KELLIMQEELAAVADEVVVVTDDGTAGRKALVTEPLKEFCGSSP
                     KPDLAVAIGPPVMMKFCAEATRPFGVRTLVSLNTIMIDGTGMCGGCRVSVGGETKFVC
                     VDGPEFDGHLVDWDGMMARLRSYKAMEEEAHHACHLRQEADRLAAEAAAGAATAAAAG
                     LCSAAGAPATGGQAPKDAAKGGER"
     gene            552..2048
                     /gene="gltA"
                     /locus_tag="PLG14_08560"
     CDS             552..2048
                     /gene="gltA"
                     /locus_tag="PLG14_08560"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOX49221.1"
                     /translation="MSEIDRTALERGAEEELAKVRALRLGGRDLSPRERLALPSQDMP
                     CQDPGARAANMFEVSEGYSEAQARLEAERCLSCRNAPCVAGCPVGVDIPGFIGRIREG
                     DFEGAARVIKRTNLLPSICGRVCPQEKQCQAPCTVGKSLGGVEKAVQIGRLERYVADR
                     ERACGLCELPDPPAPSGKRVAVVGSGPAGLTCAADLRREGHAVVVYEAFQKAGGVMVY
                     GIPEFRLPKELVRMEAETLASMGVRFELNFLVGRTRPLKALLSEDGFDAVFVGTGAGL
                     PKFMEMPGENLVGVFSANEYLTRANLMRAYDRPRAATPIYPSRRVAVLGGGNVAMDAA
                     RTALRLGAEESRVLYRRTRDEMPARAEEVVHAMEEGVAFDFLTSPTRVLAGEDGRAAA
                     LEILSYELGEPDASGRRSPVPIRGSERVVPFDTVIVAIGNESNPLLARTTPELKVDRR
                     GHILVDERQKTSIDRVYAGGDIVLGAATVILAMGEGRRAAAAINEVLQ"
     gene            2176..2847
                     /locus_tag="PLG14_08565"
     CDS             2176..2847
                     /locus_tag="PLG14_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49222.1"
                     /translation="MRRIALPLALILAVLAALPLAAQAKPDALLLYRQGRELETAGKA
                     AEAQAKYAESIAVCDVELAADSRRMDAYAVKCWSLFRQNKHQEVVNTGSAALKVQFDA
                     RVAEVMGESFYHLGQMDNAIKYLQRYLENSSDNADRVPTAYFYLGETYLRLKKYSHAD
                     IAYSTAVTREPSMPRWWYRLGQACEALGEWKRAYDAYGKALSLSPSMAEATEGQARVK
                     PKAGL"
     gene            2844..3806
                     /locus_tag="PLG14_08570"
     CDS             2844..3806
                     /locus_tag="PLG14_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOX49223.1"
                     /translation="MSAPRGPGPGGRPSCRLVLNPVAGKGRAASRLSEIESLLDARGL
                     ERDLVLTEGVGHAALLAEEAAREGRGLLVVAGGDGTANEAINGLMAARAAGLKPPALA
                     VLPVGRGNDFAHGAGFPAGLAEAVELLAKGSRRPLDVGRVTGGDYPQGRYFGNGVGVG
                     FDTIVGLEAARMKRVHGFAAYVLGAAKTFLAFPPAPSVAVSFDGRRVERRAHQISLMN
                     GCRMGGTFFMAPEAKVGDGLLDLCMSGDMTRGEMLGAILRYTKGSQSELQGFLFARSA
                     SFEIEAPDGGLVVHADGETICVNGFSLKVECLPGALELCGVRED"
     gene            complement(3884..4921)
                     /locus_tag="PLG14_08575"
                     /pseudo
     CDS             complement(3884..4921)
                     /locus_tag="PLG14_08575"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            5036..5599
                     /locus_tag="PLG14_08580"
     CDS             5036..5599
                     /locus_tag="PLG14_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49224.1"
                     /translation="MKVIRAAALVLASALVLASCASVAQGAVSGALGSLTGGISAGAP
                     SGGMAASGSAAVAVDFQSGEILCSEDSRPMMDSSFWVAKVLTPASAATKNQAEVVFVS
                     DGKKMWVNHVVASRKAVKADFQIGAPVFFLAGWSNHDKIGADSYRKDRWDLGTITSVE
                     YLYKNQVEISGDLYTIDFLRVPTDPIK"
     gene            5707..6417
                     /locus_tag="PLG14_08585"
     CDS             5707..6417
                     /locus_tag="PLG14_08585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOX49225.1"
                     /translation="MEHPEAAPFAADAAFFSTLEAETRFYIVRHGQSEANARSVVQGL
                     RDFPLDASGRAQAAAVGAWLAGEGIAEVLCSPLSRAVETARIIARAASLSEPRVEPTL
                     VELDVGVFSGLSLEEARSRFPEAYADFESRSWDGVPGAESSALLYYRAMEAWSRLRER
                     AASGSRALAVVSHGGFIQWLFRSTFGCRSWMPLLPTGNCCVFELLVAPTGGGRPAYLQ
                     WRRVNFQASPLPSVSPVF"
BASE COUNT          912 a         2432 c         2222 g          907 t
ORIGIN      
        1 aaggagctcc tcatcatgca ggaggagctc gcggccgtcg ccgacgaagt cgtcgtcgtc
       61 accgacgacg gcaccgcggg ccgcaaggcc ctggtcaccg agcccctcaa ggagttctgc
      121 ggctcctcgc cgaaaccgga cctcgcggtc gccatcggcc cgcccgtgat gatgaagttc
      181 tgcgcggagg ccacgcgccc cttcggcgtc cgcaccctgg tctccctcaa cacgatcatg
      241 atcgacggca cggggatgtg cggcggctgc cgcgtctccg tcggcggcga gaccaagttc
      301 gtctgcgtcg acgggcccga gttcgacggc cacctcgtgg actgggacgg gatgatggcc
      361 cgcctgcgct cctacaaggc gatggaggag gaggcccacc acgcatgcca cctccggcag
      421 gaggccgacc ggctcgcggc cgaggccgcc gccggagccg cgacagcagc cgcggccggg
      481 ttgtgctccg ccgccggagc cccggccacc gggggccagg cccccaagga cgccgcgaag
      541 ggaggcgagc gatgagcgaa atcgaccgga cggccctgga gcgcggggcc gaggaggagc
      601 tcgcgaaggt ccgagccctg cgcctgggcg gccgcgacct ctcgccccgc gagcgcctgg
      661 ccctcccctc ccaggacatg ccctgccagg atcccggcgc ccgcgccgcc aacatgttcg
      721 aggtctccga gggctactcc gaggcccagg ccaggctgga ggccgagcgc tgcctctcct
      781 gcaggaacgc gccctgcgtc gcgggctgtc ccgtcggcgt cgacatcccg ggcttcatag
      841 gcaggatcag ggaaggcgac ttcgagggcg ccgcgcgggt catcaagcgg acgaacctcc
      901 tgccctccat ctgcggacgc gtctgccccc aggagaagca gtgccaggcc ccctgcacgg
      961 tcggcaagtc cctgggcggc gtcgagaagg ccgtccagat cggccgcctg gagcgctacg
     1021 tcgccgaccg ggagcgggcc tgcggcctct gcgagctccc cgatcccccc gcgccgagcg
     1081 ggaagcgcgt cgccgtcgtc ggctcggggc cggccggcct cacctgcgcg gccgacctgc
     1141 gccgcgaggg ccatgccgtg gtcgtctacg aggccttcca gaaggccggc ggcgtcatgg
     1201 tctacggcat ccccgagttc cgcctcccca aggagctcgt ccgcatggag gcggagaccc
     1261 tggcctccat gggcgtccgc ttcgagctca acttcctcgt gggccgcacc cgccccctca
     1321 aggccctcct ctcggaggac ggcttcgacg cggtcttcgt gggcaccggc gcgggcctgc
     1381 cgaagttcat ggagatgccc ggggagaacc tcgtgggcgt cttcagcgcg aacgagtacc
     1441 tgacgcgcgc caacctcatg cgcgcctacg accggccccg ggccgcgacg cccatctacc
     1501 cgagccgccg cgtggcggtc ctgggcggcg gcaacgtcgc gatggacgcg gcccgcacgg
     1561 ccctgcgcct gggcgcggag gagtcgcgcg tcctctaccg ccgcacccgg gacgagatgc
     1621 ccgcccgcgc ggaggaggtg gtccacgcga tggaggaggg cgtcgccttc gatttcctca
     1681 cgagcccgac cagggtcctc gcgggggagg acggccgcgc cgccgccctg gagatccttt
     1741 cctacgagct cggcgagccc gacgcctccg gccgccggag tcctgtgccg atccgcggca
     1801 gcgagcgcgt cgtgcccttc gacacggtca tcgtcgcgat cgggaacgag tcgaaccccc
     1861 tcctggcccg gacgaccccc gagctcaagg tggacaggcg gggccacatc ctggtcgacg
     1921 agcggcagaa gacgagcatc gaccgcgtct acgcgggcgg cgacatcgtc ctgggcgcgg
     1981 ccaccgtcat cctcgcgatg ggcgaaggaa ggcgggccgc ggccgcgatc aacgaggtcc
     2041 tgcagtaggg ttgcaggggg cggtccccgg ccgcccccgg ctcgccgccg ctccgcgggg
     2101 ccgcccccgg ccttgccgag gggcgccgtt ccggggcaat atggcgggag ccacaaccca
     2161 gatccggagt agccaatgcg ccgcatcgcc cttcccctcg ccctcatcct agccgtcctg
     2221 gccgccctgc ccctcgcggc ccaggccaag cccgacgccc tcctcctcta ccgccagggc
     2281 cgggagctcg agaccgccgg caaggccgcc gaggcccagg ccaagtacgc ggagtccatc
     2341 gccgtctgcg acgtcgagct cgccgccgac tcgaggcgca tggacgccta cgcggtgaaa
     2401 tgctggagcc tcttccgcca gaacaagcac caggaggtcg tcaacaccgg ctccgcggcg
     2461 ctcaaggtcc agttcgacgc ccgcgtggcc gaggtgatgg gcgaatcctt ctaccacctg
     2521 gggcagatgg acaacgccat caagtacctc cagcgctacc tggagaattc ctcagacaac
     2581 gccgaccgcg tcccgaccgc ctacttctac ctcggggaga cctacctccg cctcaagaag
     2641 tactcccacg ccgacatcgc ctactccacg gcggtgacca gggagccctc gatgccgcgc
     2701 tggtggtaca ggctcggcca ggcctgcgag gccctgggcg agtggaagcg cgcctacgac
     2761 gcctacggca aggcgctctc cttgagcccc tccatggcgg aggccaccga gggccaggcc
     2821 cgcgtgaagc ccaaggcggg actctgagcg ccccgcgcgg cccgggcccc ggcggccggc
     2881 cctcctgcag gctcgtcctc aatcccgtcg cgggcaaggg ccgggccgcc tcccgccttt
     2941 cggagatcga gtccctcctg gacgcgcgcg gcctcgagcg cgacctcgtc ctcacggaag
     3001 gggtgggcca cgccgccctc ctcgcggagg aggcggcccg cgaaggccgc ggcctcctcg
     3061 tcgtcgcggg gggcgacggg acggccaacg aggcgatcaa cggcctcatg gccgcccggg
     3121 cggccggcct caagccgccg gccctggccg tcctacccgt ggggcggggc aacgacttcg
     3181 cccacggcgc gggcttcccg gccggcctcg ccgaggcggt cgagctcctc gccaagggct
     3241 caaggaggcc cctggacgtc ggccgcgtga cgggcggcga ctacccccag ggccgctact
     3301 tcgggaacgg cgtcggcgtg ggcttcgaca cgatagtcgg actcgaggcc gccaggatga
     3361 agcgggtcca cggcttcgcc gcctacgtcc tgggcgccgc gaagaccttc ctcgccttcc
     3421 ctcccgcccc cagcgtcgcc gtctccttcg acggcaggcg cgtcgagcgg cgggcccacc
     3481 agatcagcct catgaacggc tgccgcatgg gcgggacctt cttcatggcg ccggaagcga
     3541 aggtcggcga cggcctcctg gacctctgca tgtcgggcga catgacgagg ggcgagatgc
     3601 tcggcgcgat cctccgctac acgaagggca gccaatcgga gctccagggc ttcctcttcg
     3661 cgcgctcggc ctccttcgag atcgaggcgc ccgacggggg cctcgtcgtc cacgcggacg
     3721 gcgagacgat ctgcgtgaac ggcttctccc tcaaggtcga gtgcctgccc ggcgccctcg
     3781 agctctgcgg ggtcagggag gactgaggcg gcgcctgccc tgcctccgcc tccagcgccc
     3841 gccgcatctc ttctttgggc cctccctttc ccttcgcctc cttcgcgagg cgggccatgt
     3901 ccgcgacgaa gcgccggagg tctccgcccg acaccttgga gagatagtcg gcgtagagtt
     3961 cgggctcgcc ctcgtagagg cggtagaggt cgagataggc gttgttgagc ctctccatcg
     4021 ggaaggcctc gaagcgcgcg gtccggaaca gcgagtcgta gtcccgcgcg aagagggccg
     4081 ccctggcggc gatgacctcc gccttccgag cccgcgcctc ctccgcgggc aggcccgagg
     4141 cgtataccgc ctccagctcc tccgcggtgc cgcgcaggaa ggaggcgaag gcctccgcct
     4201 cgcggcgggc gagcctggcc tcagccaagg cgggggagtc cgacccttcg agggaggcga
     4261 ggtagcggtc ggctccctcg cggccgacga aggtggcgag ctcctcgttc cagtcccccg
     4321 cgcccttggc ataggcggtc gcgtggcaga gctcgtggat gacgaggtcc gccagctccg
     4381 cctccgagta ggagctcatg aaggagaaga gggggtcgga gagccagccg agggtggaga
     4441 aggcgtcgac gggccggacg atgacgtcca ggccctcggc gcggcgggcc gcggcctcct
     4501 tctccgcctc ctctggcctg aagaagccct tgtagggcag cttccccacg agagggtagg
     4561 accagaggtg gcggtcgaag gagagttcgg cgcaggcctg gacgaccgtc gccaggcggt
     4621 ccgcgtccag cctgacgagg ctccggtagt tcttcgtctc cttgaggccg agctcggaga
     4681 aggcgaaggc ccgcaccctc tccgccctga ggacgaggcc gcgctccgcc tccggggacg
     4741 cggggtcggc gaggacccgc tccaggggcc tggccctgga gcggaggccg aggtagcgga
     4801 agccctgggt cgtcacgtag cagcccgaga ggaagagggc ggagagggcc gccgcgccca
     4861 ggaaggccgc gcgggagagg ccgcccgagg gccggcgccg gagactgtcg ccatccgtca
     4921 tgccgcgact ctagctccgg agggcggtcc gggtaaagag agggactttc gccttgcatt
     4981 tcgcgggagc cgcgctaaaa tatgatgagc tacgagcttt tcgaaaggag actttatgaa
     5041 agtaatccgc gccgccgccc tcgtcctcgc gtcggcactc gtcctcgcct cctgcgcctc
     5101 cgtcgcccag ggagccgtga gcggagcctt gggctccctg accggcggca tctccgcggg
     5161 cgccccctcc ggcggcatgg ccgcctccgg ctccgcggcc gtcgccgtcg acttccagtc
     5221 cggcgagatc ctctgctccg aggacagccg gcccatgatg gactcgagct tctgggtcgc
     5281 caaggtcctc acccccgcga gcgccgcgac caagaaccag gctgaggtcg tcttcgtctc
     5341 cgacggcaag aagatgtggg tcaaccacgt cgtcgcgagc cggaaggccg tcaaggcgga
     5401 tttccagatc ggcgctcccg tgttcttcct ggccggctgg tcgaaccacg acaagatcgg
     5461 agccgacagc taccggaagg accgttggga cctcggcacc atcacctccg tggagtacct
     5521 ctacaagaac caggtcgaga tcagcgggga tctctacacg atcgacttcc tccgcgtccc
     5581 caccgacccg atcaagtaag ccgccttccc agctcaggat cgaggggccg tccagagggg
     5641 cggcccctcg ctatttcccg cgcggcttta ccctcggccc ggggcccggc tatactcggg
     5701 cccgccatgg aacatcccga agccgccccc ttcgccgccg acgcggcctt cttctcgact
     5761 ctcgaggccg agacgcgctt ctacatcgtc cgccacggcc agagcgaggc caacgcccgc
     5821 tccgtcgtcc agggcctcag ggacttcccc ctggacgcct cgggccgggc ccaggcggcg
     5881 gccgtcggcg cctggctcgc gggcgagggc atcgccgagg tcctgtgctc gccgctctcc
     5941 cgcgccgtcg agaccgcccg catcatcgcc cgcgcggcga gcctctccga gccccgcgtc
     6001 gagccgaccc tcgtcgagct cgacgtcggc gtcttctcgg gcctctccct ggaggaggcc
     6061 cgctcccgct ttcccgaggc ctacgccgac ttcgagtccc ggagctggga cggggtgccc
     6121 ggggccgaaa gctccgccct gctctattat cgcgccatgg aggcctggtc gcggctgagg
     6181 gagcgggccg cctcgggctc ccgggccctg gccgtcgtga gccacggcgg cttcatccag
     6241 tggctcttcc gctcgacctt cggctgccgc tcctggatgc ccctccttcc gacgggcaac
     6301 tgctgcgtct tcgagctcct cgtcgcgccc acgggcgggg gccggccggc ctacctccag
     6361 tggcgccgag tcaattttca ggcctccccc ctgccttccg tctcccccgt cttctagacc
     6421 cgccgcccga ccgggcttgg cggcggcgct ggcccgcggg cctgctgcat tag
//
LOCUS       DAOOSJ010000162         6405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7496_length_6405_cov_6.611654, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000162 DAOOSJ010000000
VERSION     DAOOSJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_6405_cov_6.611654"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLG14_08590"
     CDS             complement(<1..815)
                     /locus_tag="PLG14_08590"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49226.1"
                     /translation="MKQFDARILANRQVSESFRELVLDWRAEPPEPGQFLTLRVSPRF
                     DPLLRRPFAFSAYSPERGAASVVYQVRGSATALLSELSPGSLIDAIGPLGRPFPAPME
                     GERAILAGGGIGLGPILFLAKELERRANAGAGHEPLVLLGFRSASFVPELELPASAIL
                     CTDDGSAGFRGTPHSRLASDPSLTAPARLYGCGPAAMLAALAALARERGWEASLSAEQ
                     WMACGVGACMGCALERADGKGYLRACADGPVFGRDEIDWVAEAARAAPPARTAA"
     gene            991..2070
                     /locus_tag="PLG14_08595"
     CDS             991..2070
                     /locus_tag="PLG14_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOX49227.1"
                     /translation="MDGILQILALAAFLSGGAILARLRLAPPARLVDRLIKLVLWALL
                     FVMGFRIGNDRAIAARLGEIGLLALASAALSVAGTVAAVLAGYALIGLWRGPKGGARR
                     AAKEGAAEGAIAAATPIPAASVASAAAVRDVATPTPTASIAISAAGRSAPGSSPGRLE
                     RFKAPALLLGIVVLGFFAGLALPAAGFDFSKATGATLNALLFLIGAQFVQSGVSLKGA
                     FLRPETLMTPLATALGSLAGGLLLVPLFGLSAGKALSLSAGFGWYSLSGVLIADLGDG
                     ALGSAAFLSNMARESIAFLAIPLLARGRFPSLAIGVGGATAMDVTLPLVEQCAGPEAV
                     PASFASGALLSLSVPLLVPLFFKLG"
     gene            2289..2831
                     /locus_tag="PLG14_08600"
     CDS             2289..2831
                     /locus_tag="PLG14_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49228.1"
                     /translation="MREDDRQLGRIPLAAIGLLACLLCALAPAGGFAEAAQPSPSALS
                     VRIAGGDKAPLLLDEEAIKALPQRSFTTLDPWDGKIHEFSGVLLSVLLEKAGAPAQAA
                     KITVTARNKYTIPIRRSDCEKYGYLLAWKMDGRPFGDDKATRNRGFASIAIDFTGRPE
                     LDPELYKHQLVWQVSEIVVE"
     gene            2828..4318
                     /locus_tag="PLG14_08605"
     CDS             2828..4318
                     /locus_tag="PLG14_08605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX49229.1"
                     /translation="MRKPRVPPELAASRGSRALLYAYASIIVLLVFVVGLAYRGDLEL
                     KELAGERRVDFERNIAEQGAALTRVVFALEELLVAAAGEGWRVEPAPLAELGRSLVNH
                     ARIPMQIEPSSLEGLLGDDPLFLAERLEVSNRVYESRIGRLESLLDAAEAALGADLAS
                     GAGVASGAALGKGAAQGGLAALAALEAFLAEVRVFLPELKERSALSMQVAGAYHAANR
                     ANLAHIDEHIGWNLVELSALSALLVLVSVLIIRARLAAERELKAHKARLEAEAEVRNE
                     ELGAANESLLEALAAKDVLIKEIHHRVKNNLSMVASLISLQRDYAGPGAAEGAFEKLG
                     ERIQAISLIHEKLYRSADLSRISFGDYAAELVESLRSSLCADPEAVALDLEFEEARFA
                     PDLLLPLGLVVTELVTNSLKHAFGKEGRGRIRLSLAPAGEEWLLEVGDDGTPPADPKA
                     ILESSSLGSVLVQSLVKQVKGSLALDLSGGTNFAIRFPRNPAEGGA"
     gene            complement(4405..5337)
                     /locus_tag="PLG14_08610"
     CDS             complement(4405..5337)
                     /locus_tag="PLG14_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOX49230.1"
                     /translation="MLSFVLLSVLLGTVFLALLVSLALSLSRARIELPASAPELLLAA
                     RAAGGAARTVGGNAEAAGTAEAAGAGGPPRKPLAVCLGDSITHGVVSANYVDMLASRL
                     PDWEFSNGGVNSELAYNVASRLDPVVALEPDAVTVLIGTNDVNATFGLRSLLGYWALH
                     RLPERPSLLFFRENLAVIVRRLRRETKARVALLSMPPIGEDPGHYAWLRSEEYAEAVK
                     EVAKAEGAEYLPLRERIVAYLDSVPRGRAIPFERFGQAQGRSARSRFLYGKSFDEIAA
                     GNGFHLLVDGIHLNTHGASMAADLVEGFLRQSRR"
     gene            complement(5349..6143)
                     /locus_tag="PLG14_08615"
     CDS             complement(5349..6143)
                     /locus_tag="PLG14_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49231.1"
                     /translation="MNQDQVKGLLLDTADAETEFSVVFSGKESSVANGLYKPQSREIV
                     IHNRNFDSDDQLVYTALHEYAHHLHCERKGFVVSGRAHTNEFWGIFHELLVEAEAKGF
                     YRNVFESEPEFVDLTRRIKESCIAENGRLMIEFGKLMIEAQGLCERHKTRFEDYVDRA
                     LGVPRTTAAAASRAAAYRVDPEIGWDGLKMAAGFRDPEVRAEAVAALKGGASPAAVRA
                     RFSADKPPEEPTERLFKEKERLEKSIRMLEEKLAEVERRLAESGGA"
     gene            complement(6232..>6405)
                     /locus_tag="PLG14_08620"
     CDS             complement(6232..>6405)
                     /locus_tag="PLG14_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOX49232.1"
                     /translation="YFRQKGISFTEHNVAADPRKADEMVRRSGQMGVPVIDVNGRVIL
                     GFNQAEIERALHR"
BASE COUNT          895 a         2282 c         2306 g          922 t
ORIGIN      
        1 gcggcagtcc gggcgggcgg cgcggcgcgg gcggcctccg cgacccagtc gatctcgtcg
       61 cggccgaaga cggggccgtc ggcgcaggcg cgcaagtagc ccttgccgtc ggcccgctcc
      121 agggcgcagc ccatgcaggc gccgacgccg caggccatcc actgctcggc ggagaggctc
      181 gcctcccagc cgcgctctcg cgcgagggcg gccagggcgg cgagcatcgc ggccggcccg
      241 cagccgtaga ggcgcgcggg ggccgtcaga gatggatcgg aggcgagccg cgaatggggc
      301 gtgccgcgga agccagcgga gccgtcgtcg gtgcagagaa tggcggaggc gggcagctcg
      361 agctcgggga cgaaggaggc cgagcggaag ccgaggagga cgaggggctc atgtccggcg
      421 cccgcgttgg cgcgcctctc gagctccttc gcgaggaaga ggatgggccc gaggccgatg
      481 ccgcctccgg cgaggatcgc gcgctcgccc tccatggggg cagggaatgg cctgccgagc
      541 ggaccgatgg cgtcgatgag ggagccgggc gagagttcgg agagtagggc cgtcgcgctc
      601 ccccgcacct ggtagacgac ggaggcggcg ccgcgctcgg gggaataggc ggaaaaggcg
      661 aagggtctgc ggaggagggg atcgaagcgc ggcgagacgc gcaaggtgag gaactggccg
      721 ggctctggcg gctcggcccg ccagtcaagg acgagctccc tgaaggattc cgagacctgg
      781 cggttcgcga ggatgcgagc gtcgaattgc ttcactcggg atgccccctc tatcaagcgc
      841 cacagtagcc gaggcctccc ggcctgtcaa tcgacgcggg cctccgtggc gccgtcgggc
      901 aggggaggcg cctccgggca ggggaggcgc tgcgggagcg gcgaagcgag gcttgccgcg
      961 gcgggccgca agcggctatc ctcacccacc atggacggaa tcctgcagat actcgccctc
     1021 gccgccttcc tctcaggcgg cgccatcctc gcgcggctca ggctcgcgcc gcctgcgcgc
     1081 ctcgtcgacc gcctcatcaa gctggtcctc tgggccctcc tcttcgtcat gggcttcagg
     1141 atcggcaacg accgcgccat cgccgcgagg ctcggcgaga tcggcctcct cgccctggcc
     1201 tccgccgcgc tctccgtcgc ggggacggtc gcggccgtcc tcgcgggcta tgccctcata
     1261 ggcctctggc gcgggcccaa ggggggcgcg cggcgggccg ccaaggaagg cgcggccgag
     1321 ggagccatcg ctgccgcgac gccgatcccc gcggcgagtg tcgcctccgc cgccgccgtg
     1381 agggatgtcg ctacgccgac ccccacggcg agcatcgcca tcagcgccgc agggcgctcc
     1441 gcgcccggct cctcgcctgg ccgcctcgag cgcttcaagg cgccggccct cctcctcggc
     1501 atcgtcgtcc tcggcttctt cgccggactc gccctgcccg ctgccggctt cgatttctcc
     1561 aaggcgacgg gggccaccct caacgccctc ctcttcctca tcggggccca gttcgtccag
     1621 tcgggcgtgt cgctcaaagg ggccttcctc aggcccgaaa ccctgatgac gcccctggcc
     1681 acggctttgg gcagcctcgc tggcggcctc ctcctcgtgc ccctcttcgg cctctcggca
     1741 ggcaaggccc tctcgctctc cgcgggcttc ggctggtaca gcctctcggg cgtcctcatc
     1801 gccgacctgg gcgacggcgc cctgggctcg gcggcctttc tttccaacat ggcgcgcgag
     1861 tcgatcgcct tcctcgcgat ccccctcctc gcgcgcggtc gcttcccctc cctcgcgata
     1921 ggcgtcggag gggcgacggc gatggacgtg acccttccgc tcgtcgagca gtgcgccggg
     1981 ccggaggccg tgcccgcgag cttcgcctcg ggcgccctcc tctcgctctc cgtgcccctc
     2041 ctcgtgccgc tcttcttcaa gctcggctga ggcggcccgc gcaagcgcct cagcttgcgt
     2101 ccgcgggcga acgccgaccg cgccccgagc cgaccaacgc cccgccgccc ttcagggaga
     2161 gcgccgccgg ctcgcccgcc gcctcgaaag gcaaggccct gccggccggc gaccttgacc
     2221 gcgaggcggg cgggaagtag tgttatagtc cgaggatcga agcggcgcga gccgcgcgag
     2281 gggagtccat gagggaagac gatcgacagc ttggacggat accgctcgcg gccatcggcc
     2341 tccttgcctg cctgctctgc gcgctcgcgc cggccggcgg cttcgccgag gcggcccagc
     2401 cctctccctc cgcgctctcc gtccggatcg ccggcggaga caaggccccg ctcctcctgg
     2461 acgaggaggc gatcaaggct cttccccaga gaagcttcac gaccctcgat ccctgggacg
     2521 ggaagatcca cgagttctcg ggcgtcctcc tctcggtcct gctggaaaag gcgggagcgc
     2581 cggcccaggc cgcgaagatc accgtgaccg ccaggaacaa gtacacgatc ccgatcaggc
     2641 gctcggactg cgagaagtac ggctacctcc tggcctggaa gatggacggc cggcccttcg
     2701 gcgacgacaa ggcgacgagg aaccgcggct tcgcgagcat agcgatcgac ttcacgggcc
     2761 gccccgagct cgatcccgag ctctacaagc accagctcgt ctggcaggtg agcgagatcg
     2821 tcgtcgaatg aggaagccgc gcgtgccgcc cgagctcgcg gcgagccgcg gctcgcgcgc
     2881 cctgctctac gcctacgcct cgatcatcgt actcctggtt ttcgtcgtgg gcctggccta
     2941 ccgcggggac cttgagctca aggagctcgc gggcgagcgg cgagtggatt tcgagcggaa
     3001 catcgcggaa cagggcgcgg ccctcaccag ggtggtcttc gccctggagg agctcctggt
     3061 cgcggccgca ggggaaggct ggcgggtcga gcccgcgccc ctcgcggagc tcggccggag
     3121 cctggtcaac cacgcgcgca tacccatgca gatcgagccc tcttccctgg agggcctcct
     3181 gggggacgac ccgctcttcc tggcggagcg cctcgaggtc tccaaccgcg tatacgaatc
     3241 gcggatcggg aggctcgaga gcctcctgga cgcggcggaa gcggcgcttg gagcggatct
     3301 tgcttcaggc gcgggcgtcg cctctggcgc ggccttgggc aagggcgcgg cccagggcgg
     3361 actcgccgcc ctggcggcgc tcgaggcctt cctcgccgag gtccgcgtct tcctccccga
     3421 gctcaaggag cgctcggccc tctccatgca ggtcgccggc gcctaccacg cggccaaccg
     3481 ggcgaacctg gcccacatcg acgagcacat aggctggaac ctcgtcgagc tgtcggccct
     3541 gagcgccctc ctcgtcctcg tgtccgtcct gatcatccgc gcccgccttg ccgcggagcg
     3601 ggagctcaag gcccacaagg cgcgcctgga ggcggaggcc gaggtccgga acgaggagct
     3661 cggcgccgcg aacgaaagcc tgctcgaggc cctggcggcg aaggacgtcc tcatcaagga
     3721 aatccaccac cgcgtcaaga acaacctctc catggtcgcg agcctcatct ccctgcagcg
     3781 ggactacgcg gggccggggg ccgcggaggg ggccttcgag aagctgggcg agcgcatcca
     3841 ggccatctcc ctcatccacg agaagctcta ccggagcgcc gacctttcgc gcatttcctt
     3901 cggcgactac gccgcggagc tggtggagag cctccgctcc tccctttgcg ccgatcccga
     3961 ggcggtggcc ctggacctcg agttcgagga ggcccgcttc gcccccgacc tcctgctccc
     4021 gctgggcctc gtcgtcaccg agctcgtgac caactccctg aagcacgctt tcgggaagga
     4081 aggccgaggg aggatccgcc tctccctcgc cccggcgggg gaggaatggc tcctagaggt
     4141 cggcgacgac ggaacgccgc ccgccgaccc caaggccatc ctcgagagca gctcgctcgg
     4201 ctcggtcctg gttcagagcc tcgtgaagca ggtgaagggg agcctcgcgc tggacctctc
     4261 gggcggcacg aatttcgcga tccgcttccc ccggaacccg gcggagggcg gggcatagcg
     4321 cccgagagcc ggccgcggcg agggcgggcg gcgctccgcc ccccggcgcc ttcgcggcgg
     4381 gccctgcccg cggcgcccgt accgtcagcg cctcgactgc ctcaggaagc cctcgaccag
     4441 gtccgcggcc atcgaggcgc cgtgggtgtt gaggtggatg ccgtcgacga ggagatggaa
     4501 gccgttcccg gccgctatct cgtcgaagct cttcccgtag aggaagcggc tccgcgccga
     4561 gcggccctgg gcctggccga agcgctcgaa ggggatggcc cggccccgcg ggaccgagtc
     4621 gaggtaggcg acgatccgct cgcgcagggg gaggtactcg gccccctccg ccttcgcgac
     4681 ctcctttacc gcctccgcgt attcctcgga cctgagccag gcgtagtggc cggggtcctc
     4741 gccgatgggc ggcatggaga ggagggcgac gcgcgccttg gtctcgcgcc tcaggcgtcg
     4801 cacgatcacg gccaggttct ctcggaagaa gaggaggctc ggccgctcgg gcaggcggtg
     4861 gagggcccag tagcccagga gggagcggag gccgaaggtc gcgttgacgt cgttcgtgcc
     4921 gatgaggacc gtgaccgcgt cgggctccag ggcgacgacg gggtccaggc gggaagcgac
     4981 gttgtaggcg agctccgagt tgacgccgcc gttggagaat tcccagtcgg gaaggcggga
     5041 ggcgagcatg tcgacgtagt tcgcgctcac gacgccgtgc gtgatcgagt cgcccaggca
     5101 gaccgcgagg ggcttccgcg gcggcccgcc cgcgcccgcg gcctcagcgg tccccgcggc
     5161 ctcagcgttt ccgccgacgg tccgcgcggc gccccccgcg gcccgggccg cgaggaggag
     5221 ctcgggggcg ctcgccggga gctcgatccg cgcgcgggag agggagaggg ccagggacac
     5281 gaggagggcc aggaatacgg tccctaggag gacggagagg aggacgaagg agagcatcgc
     5341 ggcgctccct aggccccgcc cgactcggcc aggcggcgct cgacctccgc gagcttctcc
     5401 tccagcatgc ggatgctctt ctccaggcgc tccttctcct tgaagagccg ctcggtcggc
     5461 tcctcggggg gcttgtccgc cgagaagcgg gcgcggacgg cggccgggct cgccccgccc
     5521 ttgagggccg cgacggcctc cgccctgacc tcgggatcgc ggaagcccgc ggccatcttg
     5581 aggccgtccc agccgatctc gggatcgacg cgataggcgg cggcgcggga ggcagcggcg
     5641 gccgtcgtcc tgggcacgcc cagggcgcgg tccacgtagt cctcgaagcg ggtcttgtgg
     5701 cgctcgcaga gcccctgggc ctcgatcatg agcttgccga actcgatcat gagcctgccg
     5761 ttctcggcga tgcaggactc cttgatgcgg cgcgtcaggt ccacgaactc gggctcgctc
     5821 tcgaagacgt tgcggtagaa gcccttcgcc tcggcctcca cgaggagctc gtggaagatg
     5881 ccccagaact cgttcgtgtg ggcgcggccg gagacgacga agcccttgcg ctcgcagtgg
     5941 aggtggtggg cgtactcgtg gagggccgtg tagacgagct ggtcgtcgga gtcgaagttg
     6001 cggttgtgga tgacgatctc gcggctctgc ggcttgtaga gcccgttggc cacggagctc
     6061 tccttgccgg agaagacgac ggagaactcc gtctccgcgt cggccgtgtc gaggaggagg
     6121 ccttttacct ggtcctggtt catgccgcca tcatagggac ggcggcccgc gaaggcaagc
     6181 ggcggaggct cagcggtgga agctcagcgg cggaggctca gcggtgaagg ctcagcggtg
     6241 gagggcccgc tcgatctccg cctggttgaa gccgaggatg acccggccgt tcacgtcgat
     6301 caccggcacg cccatctggc ccgagcggcg caccatctcg tcggccttcc gggggtcggc
     6361 cgcgacgttg tgctccgtga aggagatgcc cttctgcctg aagta
//
LOCUS       DAOOSJ010000163         6316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7652_length_6316_cov_6.272640, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000163 DAOOSJ010000000
VERSION     DAOOSJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7652_length_6316_cov_6.272640"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..794
                     /locus_tag="PLG14_08625"
     CDS             174..794
                     /locus_tag="PLG14_08625"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOX49233.1"
                     /translation="MPSIDKAEARRRARALAASLPPAAVAEESRAAAALLLSRPEFAE
                     AELLLVFLSMPGEIDTAPLVEAALAAGKRVAAPRIEGGDLAFVPLGRDWRDWPPDRWG
                     IPDPPASARALGTAELGRLRVLVAAPGLLFDAEGRRLGRGKGFYDRFLRGAREAAREG
                     GGSLVACGYALSVQLVPEAPAGPGDEAVDLVIAGGSLSRTRTTTRS"
     gene            797..1201
                     /locus_tag="PLG14_08630"
     CDS             797..1201
                     /locus_tag="PLG14_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX49234.1"
                     /translation="MGVIMRGRSTGPTTAELVHESGFVIKTTAPKDNGGDGSSFSPTD
                     LCAVSLGACGTTIMAMFARDKGIPLAGVEFELRKEMAASPRRIARIEIAYRLKGTLSD
                     KEFAMVVAAGKTCPVRVTFADRVEVVESYERA"
     gene            complement(1244..1447)
                     /gene="thiS"
                     /locus_tag="PLG14_08635"
     CDS             complement(1244..1447)
                     /gene="thiS"
                     /locus_tag="PLG14_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOX49235.1"
                     /translation="MTITLNAQSESYPERSLTVREILARKRWSFPLVIARLNGELVPR
                     EAYAERSVSDGDALELYHLVSGG"
     gene            complement(1444..2043)
                     /gene="thiF"
                     /locus_tag="PLG14_08640"
     CDS             complement(1444..2043)
                     /gene="thiF"
                     /locus_tag="PLG14_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOX49236.1"
                     /translation="MTREAARARLAKAYVGVAGCGGLGSNCAAALARAGVGRLLVADF
                     DLVSESNLDRQFFFRDQVGRPKALALAENLRRIDPGVRVESRVARIGPAEALEFFSGC
                     DAVVEAFDEAEAKAMLVETVLCRLPGTWLVAASGLAGYGRSGALKVRASGRFVICGDL
                     ESEVGPESPPFAPRVGIVANMEANAVLSILLGGGPGEEA"
     gene            2139..4127
                     /locus_tag="PLG14_08645"
     CDS             2139..4127
                     /locus_tag="PLG14_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX49237.1"
                     /translation="MQESNMAAGKSRAVWSSFVRWLFGRGGMKSMILVPITLVIGLGF
                     TASWILYVQGSRDAVFAAVDGLVLESAMREASRVGSYVDSAMRLAEANAAFLGSFPGA
                     SGRTDAIRKSFRLQLLARSEVDIVSVGFADGEYAEAQRLPGGAVRTGGAGKATGGALV
                     LERVDERGEPVSVELRRPGYDPRERPWYKRAAAAARPVWTEPYPVLSTGELTMAAVVP
                     LMEDGRVAAVATADIGLGRLSEFLGSVAEIPGCLAVLLDEGNLLVAGSRHDEGLTASG
                     PEGRLALADLDPVLRAAVEAGARSPGKVVSVAPGGRPYRAVLLDLGAGGPVPWRLAVA
                     FPEDHFLAPLHAIDLRVALVLLFMLAGNLAIAFVVAHRVADPLRRLGRAVSSFEPGSG
                     SSAGEAEALAGRSDEIGHLAVSFLSLADRLDGSFSAIRASLAEKEILLKEIHHRVKNN
                     LQIVSSLVSFQGGAAASPLARETVERLQERIQAMAYVHEEVYLSGNFEAVDMGRYLLR
                     VGESLCGGSLGASGPGWLGGRCAIGIKVEAGGLGLGLDRAIPLGLVANELVANALKHA
                     FKGRSEGTVSIRFLEEGDDYRLEVADDGVGLGASRRTGEGEAGLDEAASFPRRPDGSG
                     GLGGSLVEGLASQLRGRVEYSSGAWGSLVAVSFPRVET"
     gene            4271..4729
                     /locus_tag="PLG14_08650"
     CDS             4271..4729
                     /locus_tag="PLG14_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX49238.1"
                     /translation="MIKYLKTHDEKCVGCMTCTAVCSKLYFKEENPAKSDIQVQELGA
                     GKFHLVVCDQECRKCVAECPTQAITVSKTGVVMINKGLCVGCLACVAVCPIGAMRYYP
                     GEKHPFKCVACGACAKKCPKEALEVAEKEESPASRAWAEAAAKVRKEGQE"
     gene            4726..>6316
                     /locus_tag="PLG14_08655"
     CDS             4726..>6316
                     /locus_tag="PLG14_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOX49239.1"
                     /translation="MSLEKKDFRLLSERRYEKGSVRKGYNMRTLHLDVGSRKVTEKPV
                     SETMKAKFVGGKGFGLKLLWDGTKPSTKWDSPENEIVIAMGPVCGNTNYPGSGKSLVV
                     SLSPMTGVPIDSNVGGYFGPYLKFSGFDALELQGKAEVETIVFIDGNEGLVQFYEAPA
                     GLHGDTHLLAEELTRAFAASQDPADLQAVSVVSAGRGADHSPMGLLNFSLYDKRRAGV
                     RVKQAGRGGIGTVFRDKKLRALVCRFRGVEQNSNDAADPAAVQRLGLKLHREIVVNDD
                     KQCKMRKQGTAHLMEVMNDYDLLPTKNHKYGQDPRGPEIASWVWEKLLSQDLPDGCWY
                     GCTMACAHAVDKFELETGPYKGQKVCVDGPEYETAAGVGSNLYVYDPHGILEINFYCD
                     TYGIDTISFGTMTSFLMECWEHGVLNKDRTDGIDLSWGNWKGACQILHDMADGKRFGV
                     LAGKGVKFLAEHFAKEYGADAQFMKDIALHGKGLEQSEYISKESLAQQGGYYLTNKGP
                     QHDEAWLIFMDMVNNRIPTFEN"
BASE COUNT          951 a         2323 c         2152 g          890 t
ORIGIN      
        1 aggcgcgagg ccggcctctg cggctgcatc cacaagccct ggcgcaccga cgagttggcc
       61 gccctcatcg agtcctgcct catggaccgg gcctccgaga gctgcggctg agacgggctc
      121 cgccctcgcc ggcttcgctt gcccccggcg gccaggcggg ctagaatccc cccatgccct
      181 ccatcgacaa ggccgaggcc cggcgccggg cccgcgccct cgccgcctcc ctgcctcccg
      241 cggccgtggc cgaggagagc cgcgcggccg cggccctcct cctatcccgg cccgagttcg
      301 ccgaggcgga gcttctcctc gtcttcctct cgatgcccgg cgagatcgac accgctcccc
      361 tggtcgaagc cgccctggcg gcggggaagc gcgtcgcggc cccccgcatc gagggcggcg
      421 acctggcctt cgtgcccctg gggcgggact ggcgcgattg gcccccggac cgctggggca
      481 tccccgatcc gcccgcctcg gcccgggccc tcgggaccgc cgagctcggg aggctgcgcg
      541 tcctcgtcgc cgcgccgggc ctcctcttcg acgccgaggg acggcggctc gggcggggca
      601 agggcttcta cgaccgcttc ctccgcgggg cccgcgaggc cgcgcgggaa ggcggcggga
      661 gcctcgtcgc ctgcggctac gccctctccg tccagctcgt gcccgaggct cccgccggcc
      721 ccggggacga ggcggtcgac ctcgtgatcg ccggcgggag cctttccagg acgcggacaa
      781 cgacaaggag ctgaacatgg gagtcatcat gcgcggccgg tccaccggcc ccaccaccgc
      841 cgagctcgtc cacgagtcgg gcttcgtcat caagaccacc gcgcccaagg acaacggggg
      901 cgacggctcg agcttctcgc ctaccgacct ctgcgcggtc tcgctgggcg cctgcggcac
      961 gaccatcatg gcgatgttcg cgcgcgacaa gggcatcccc ctcgcgggcg tggagttcga
     1021 gctccggaag gagatggcgg cctcgccgcg ccggatcgcg cggatcgaga tcgcctatcg
     1081 actcaagggg acgctctcgg acaaggaatt cgccatggtg gtggccgcgg gcaagacctg
     1141 ccccgtccgc gtcaccttcg ccgaccgcgt ggaggtcgtc gagagctacg agcgggccta
     1201 gtccgggacc gccggccccg cgccgcgccc ttgcggctcg ggcctagccc ccgctcacga
     1261 ggtggtagag ctcgagggcg tcgccgtcgg agacactcct ctcggcgtag gcctcccgcg
     1321 ggacgagctc gccgttcagc ctcgcgatga cgagggggaa ggaccagcgc ttgcgcgcca
     1381 ggatctcccg gaccgtgagg ctccgctccg gatagctctc gctctgggcg ttgagggtga
     1441 tggtcatgct tcctctcccg gcccgccgcc aaggagtatc gagaggacgg cgttggcctc
     1501 catgttcgcg acgatgccga cccgcggggc gaagggcggg ctctcggggc ccacctccga
     1561 ctcgaggtct ccgcagatga cgaagcggcc cgaggccctg accttgaggg ccccgctgcg
     1621 gccgtagccc gcgaggcccg aggccgcgac gagccaggtc ccgggcagcc ggcaaaggac
     1681 cgtctccacg agcatggcct tcgcctcggc ctcgtcgaag gcctccacga cggcgtcgca
     1741 gcccgagaag aactccaggg cctcagcggg gcctatcctc gcgaccctgg actccaccct
     1801 gacgccgggg tcgatgcgcc tgaggttctc cgcgagggcc agggccttgg gccggccgac
     1861 ctggtcgcgg aagaagaact gccggtccag gttcgactcg gacacgaggt cgaagtccgc
     1921 gacgaggagg cggccgacgc cggcgcgggc cagggcggcg gcgcagttgg agcccaggcc
     1981 cccgcagccc gccacgccga cataggcctt cgcgagcctg gcccgcgccg cttctctcgt
     2041 catgggccaa gcataccggg gctttggggg ccgcggcaag ggaagggccg ccggggacga
     2101 attttgcgga cgccggagcc cggacggcgt atactccaat gcaggaatcg aacatggcgg
     2161 cgggaaaatc gagagctgtg tggtcgagct tcgtccggtg gctcttcgga cggggcggca
     2221 tgaagagcat gatcctcgtg cccatcaccc tcgtcatagg cctgggcttc acggcgagct
     2281 ggatcctcta cgtccagggc tcgcgcgacg ccgtcttcgc ggccgtcgac ggcctggtcc
     2341 tcgagtcggc catgcgcgag gccagcaggg tcggctccta cgtcgactcg gcgatgcgcc
     2401 tggccgaggc caacgccgcc ttcctgggct ccttccccgg cgcctcgggc aggacggacg
     2461 ccatccgcaa gtccttcagg ctccagctcc tggcccgctc cgaggtcgac atcgtctccg
     2521 tcggcttcgc ggacggagag tacgcggagg cccagcgcct gccgggcggc gccgtccgca
     2581 cgggcggcgc gggcaaggcg acgggcggcg ccctcgtcct ggagcgagtc gacgagcggg
     2641 gcgagcccgt ctccgtcgag ctacggcggc cgggctacga tccccgcgag cggccctggt
     2701 acaagcgggc ggcggccgcg gcccgccccg tctggaccga gccctatccc gtcctctcca
     2761 cgggcgagct cacgatggcc gccgtcgtcc ccctcatgga ggacggccgg gtcgcggcgg
     2821 tcgcgacggc cgacatcggc ctcgggcgcc tctccgagtt cctgggcagc gtcgcggaga
     2881 ttccgggctg cctggccgtc ctcctggacg aggggaacct cctggtcgcg ggctcccgcc
     2941 acgacgaggg cctgaccgcc tcggggcccg agggccggct cgcgctggct gacctggacc
     3001 ccgtcctcag ggcagccgtg gaggcgggag cccgctctcc cggcaaggtc gtctccgtgg
     3061 cgccgggagg gcgcccctac cgcgccgtcc tcctcgacct gggcgcgggc ggccccgtcc
     3121 cctggcgcct cgccgtggcc ttccccgagg accacttcct cgcgccgctc cacgcgatag
     3181 acctccgcgt cgccctggtc ctcctcttca tgctggccgg caacctggcc atagccttcg
     3241 tcgtcgcgca ccgcgtcgcc gatcccctgc gcaggctcgg aagggccgtg tcctccttcg
     3301 agcccggctc cggctcttct gcgggagagg ccgaggccct ggcgggacgg agcgacgaga
     3361 tcggccacct ggccgtctcc ttcctctccc tggccgaccg gctggacggg agcttctcgg
     3421 cgatccgcgc ctccctcgcg gagaaggaga tcctcctcaa ggagatccac caccgcgtca
     3481 agaacaacct ccagatcgtc tccagcctcg tgagcttcca gggcggcgcg gccgcgagtc
     3541 ccctggcgcg cgagaccgtc gagcgcctcc aggagcggat ccaggccatg gcctacgtcc
     3601 acgaggaggt ctacctctcc ggcaacttcg aggcggtgga catgggccgc tacctcctgc
     3661 gcgtcggcga gtccctctgc ggcgggagcc tgggcgcctc gggcccgggc tggctgggcg
     3721 ggcgttgcgc catcgggatc aaggtggagg cggggggcct cggcctggga ctggatcgcg
     3781 ccatccccct gggcctcgtc gcgaacgagc tcgtcgcgaa cgccctgaag cacgccttca
     3841 agggcaggag cgaggggacc gtgtcgatcc gcttcctgga agagggcgac gactaccgtc
     3901 tggaggtcgc cgacgacggg gtgggcctgg gcgcctcgcg gcggaccggg gaaggcgagg
     3961 ccggcctgga cgaggccgcg tccttccccc gccggcccga cgggagcgga ggcctgggcg
     4021 gcagcctggt cgaggggctc gcgtcccagc tcaggggcag ggtcgagtac tcgagcggcg
     4081 cctggggaag ccttgtggcc gtcagcttcc cccgcgtcga gacctgattg catatttata
     4141 cggcattgtg aattagcgcg tcgctttgcc cgagctcgtt gcggaaaacc gcggccgggg
     4201 gtatactgcc tcttccctgg aacgccccgc gagcgcgggg cccccgacca gcccaaggag
     4261 gcccttcacc gtgatcaaat acctcaagac ccacgacgag aaatgcgtcg gctgcatgac
     4321 ctgcacggcc gtctgctcga agctctactt caaggaagag aaccccgcga agtccgacat
     4381 ccaggtccag gagctcggcg cgggcaagtt ccacctggtc gtctgcgacc aggagtgccg
     4441 caagtgcgtc gccgagtgcc cgacccaggc catcaccgtc tccaagaccg gcgtcgtcat
     4501 gatcaacaag ggcctctgcg tcggctgcct ggcctgcgtg gccgtctgcc cgatcggcgc
     4561 gatgcgctac taccccggcg agaagcaccc cttcaagtgc gtggcctgcg gcgcctgcgc
     4621 gaagaagtgc ccgaaggagg ccctggaggt cgccgagaag gaagagagcc cggcctcccg
     4681 cgcctgggcc gaggccgccg ccaaggtcag gaaggagggc caggaatgag cctcgagaag
     4741 aaggatttca ggctcctctc ggagcggcgc tacgagaagg gctccgtccg caagggctac
     4801 aacatgcgga ccctccacct ggacgtcggc tcgcgcaagg tcacggagaa gcccgtctcc
     4861 gagacgatga aggcgaagtt cgtcggcggc aagggcttcg gcctcaagct cctctgggac
     4921 ggcacgaagc cctccacgaa gtgggacagc cccgagaacg agatcgtcat cgcgatgggc
     4981 cccgtctgcg gcaacacgaa ctaccccggc tcaggcaaga gcctcgtcgt ctccctctcc
     5041 ccgatgacgg gcgtgcccat cgactccaac gtcggcggct acttcggccc ctacctcaag
     5101 ttctccggct tcgacgccct ggagctccag ggcaaggccg aggtcgagac catcgtcttc
     5161 atcgacggca acgagggcct cgtccagttc tacgaggcgc ccgcgggcct ccacggcgac
     5221 acccacctcc tcgcggagga gctcacccgc gccttcgccg cgagccagga tcccgccgac
     5281 ctccaggccg tctccgtcgt ctccgctggc cgcggcgccg accactcgcc gatgggcctc
     5341 ctcaacttct ccctctacga caagcgccgc gcgggcgtcc gcgtgaagca ggcgggccgc
     5401 ggcggcatcg gcaccgtctt ccgcgacaag aagctccgcg ccctggtctg ccgcttccgc
     5461 ggcgtggagc aaaactccaa cgacgccgcc gacccggccg ccgtccagcg cctgggcctc
     5521 aagctccacc gcgagatcgt cgtcaacgac gacaagcagt gcaagatgcg caagcagggc
     5581 acggcccacc tcatggaggt catgaacgac tacgacctcc ttccgaccaa gaaccacaag
     5641 tacggccagg acccccgcgg ccccgagatc gcgagctggg tctgggagaa gctcctctcc
     5701 caggacctgc ccgacggctg ctggtacggc tgcacgatgg cctgcgccca cgcggtggac
     5761 aagttcgagc tggagaccgg cccctacaag ggccagaagg tctgcgtcga cggccccgag
     5821 tacgagaccg cggccggcgt aggctcgaac ctctacgtct acgacccgca cggcatcctc
     5881 gagatcaact tctactgcga cacctacggc atcgacacga tcagcttcgg cacgatgacg
     5941 agcttcctca tggagtgctg ggagcacggc gtcctcaaca aggaccggac cgacggcatc
     6001 gacctctcct ggggcaactg gaagggcgcc tgccagatcc tccacgacat ggcggacggg
     6061 aagcgcttcg gcgtcctcgc gggcaagggc gtcaagttcc tggcggagca cttcgcgaag
     6121 gagtacggcg ccgacgccca gttcatgaag gacatcgccc tccacgggaa gggactggag
     6181 cagtccgagt acatctccaa ggagtccctg gcccagcagg gcggctacta cctcacgaac
     6241 aaggggccgc agcacgacga ggcctggctc atcttcatgg acatggtgaa caaccgcatc
     6301 ccgaccttcg agaaca
//
LOCUS       DAOOSJ010000164         6261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7726_length_6261_cov_4.598292, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000164 DAOOSJ010000000
VERSION     DAOOSJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7726_length_6261_cov_4.598292"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /gene="pnp"
                     /locus_tag="PLG14_08660"
     CDS             complement(<1..1477)
                     /gene="pnp"
                     /locus_tag="PLG14_08660"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOX49240.1"
                     /translation="MVQKLSYKAGGEELILETGRMAKQANGSVFAQLGGSAVIATACC
                     GSTAQEGLDFVPLTVEYNEKYYAAGKIPGGFIKRETRPKDREILVSRIIDRPMRPLFD
                     KAFGREIQVVPTCISTDSVNPPDILAINAASAAVHISDIPFAGPIAGVRVCLLDGAYV
                     VNPTYDQIAKAKLEIVVAGTKHGITMVEGDSLEVSEEEMLGAIEVAKKPIAEICAMIE
                     ELGRLCGKKKLPLAPLKVVLNRKAEIESYARPVLTKALFTKEKMERQAAVSAAIADAK
                     AKFADALTDEVQKKLFGALMEDLMYEILRGGILNEGKRVDGRGLEDIRPITCEINVLP
                     RTHGSALFTRGETQALAVTTLGTVFDEQIFDDIEGDRRERFMLHYNFPPFSVGETGRL
                     GTGRREIGHGNLARRSIEKVLPSKERFPYTIRVVSEILESNGSSSMASVCGTSLALMD
                     AGVPIKAPVAGIAMGLVLEEGKFEVLTDILGDEDHLGDMDFK"
     gene            complement(1619..1888)
                     /gene="rpsO"
                     /locus_tag="PLG14_08665"
     CDS             complement(1619..1888)
                     /gene="rpsO"
                     /locus_tag="PLG14_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOX49241.1"
                     /translation="MALTKEDKARVVLEYGKSEKNTGSTETQVALLTERINGLTDHFK
                     TNKKDTNSRRGLLMLVGQRRRLLKYMQRHDLAGYRALIEKLNIRK"
     gene            complement(1908..2702)
                     /locus_tag="PLG14_08670"
     CDS             complement(1908..2702)
                     /locus_tag="PLG14_08670"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOX49242.1"
                     /translation="MKVVSWEEFRETWRPERPLAATVGVFDGLHLGHRSLIRRVLARS
                     PRATPAVFTFAENPKRILRPSSYHGDLASLEQKLEGFESLGLELAVLIDFSGNFSKLA
                     GRDFLSLLRDRGGLSYLAVGSNFRCGHRLDTDAAAIVEFYSSRGVAAEIVEPLEWNGH
                     PVSSSRVRSAVLDGRLEDAAAMLGRPYELDLRRAEALESGDGFASFLLPAGRAAPPEG
                     RYVAEARGSGLRAETILELGEGRVLFGTAAGQRPESVAFIKLTKEL"
     gene            complement(2699..3718)
                     /gene="truB"
                     /locus_tag="PLG14_08675"
     CDS             complement(2699..3718)
                     /gene="truB"
                     /locus_tag="PLG14_08675"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOX49243.1"
                     /translation="MTRLSGLAAIEKRAGLTSFAALGPLKRGLGTGKVGHAGTLDRFA
                     TGLLVVLVGGYSRLAPYFTALDKRYSALVRFGSETSTLDPEGEVVAEAPPPSLAKLES
                     VLPAFRGPILQAPPAYSAIHLDGKRAYERVLAGEEVEMAPRPVVIHELELLSYDGRDA
                     RLSVRCSSGTYIRSLARDLALAAGSRASLAALERGAIGPILLSEAVLPEDFEPGRDLR
                     LLDPALAAALGLRPRRLEDPARRAFQNGGRLSPRCLSDIEGVAAGARAAVRGSAAADE
                     AAAGAGTSAAAENQAAAAGAGTSAVSAAGESLSAVFSGDGAFLGLVREAAESLEYRMV
                     LGGEA"
     gene            complement(3715..4113)
                     /gene="rbfA"
                     /locus_tag="PLG14_08680"
     CDS             complement(3715..4113)
                     /gene="rbfA"
                     /locus_tag="PLG14_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOX49244.1"
                     /translation="MDEIRHKKLEEQLRGEIASLILSGEVKDPRVGAFVSVSRVEAAR
                     DLSAAKVYVSVFGLASGGEAGGDAESQAEAEAQRLDLAVEGLSRASGYIQAQVGRRLR
                     TRLTPKLVFVADRGIKEGFELNERIKGLFT"
     gene            complement(4094..>6261)
                     /gene="infB"
                     /locus_tag="PLG14_08685"
     CDS             complement(4094..>6261)
                     /gene="infB"
                     /locus_tag="PLG14_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOX49245.1"
                     /translation="PQGGGYGGPRPGYGQGGPSGGGYGGPRPGYGQGGGYGGQRPGGY
                     GQGGYGQRPGGPGRPGGYGQRPGGPGRPGGPGGSSMGAGTGKTAVRKPVRGKKPAYTK
                     REEEAEKALRLKKKQEAQAMAIPKEIDIMETISVSDLAKKMNLKPADLIANLMKLGVM
                     ATINQKIDSDTATLLAGEYGCEVHIVSLYDETVIEKVADLPGELQHRPPVVTVMGHVD
                     HGKTKLLDAIRKTDVVAGEFGGITQHIGAYQVETPKGKITFLDTPGHAAFTKMRARGA
                     QVTDIVVLVVAADDGVMPQTREAIDHAKAANLPIIVAVNKIDLPEANPDRVMSQLAEL
                     GLTPEAWGGTTIFCQISALQKRGIPELLDAILLQAEVLELTANYDSRAEAKVVESRVD
                     QGRGIVSTVIVERGTLRIGDSFAAGIYSGKVRALFNDKGERIEEATPSMPVEVIGFEG
                     LPNAGDPFEVVEDEKYARQISMKRQELKKYEESKNVKKVTLDNLYSTIQQGDVQELKV
                     IIKGDVQGSVEALKGLLEKLSTPEVRLNVIRSAAGTISEDDVNMAAASEAIIIAFNVR
                     PSPAAKLLADREKVDIRKYNIIYRAEEEIKKAMEGMLAPELKESEIGQVEVRETFQVP
                     KVGVVAGCFVLEGCVRRNCQVRVIREGVEIHQGKINSLKRFKDDAKEVAAGYECGIGI
                     EGLQDVKAGDTLEIYEMIEIARTLSAPADAGKANGRDQA"
BASE COUNT          866 a         2036 c         2285 g         1074 t
ORIGIN      
        1 ccttgaagtc catgtcgccg aggtggtcct cgtcgcccag gatgtcggtc agcacctcga
       61 acttgccttc ctcgagcacc aggcccatcg cgatgcccgc gaccggcgcc ttgatcggca
      121 cgccggcgtc catcagcgcg aggctggtgc cgcacaccga cgccatcgag ctcgagccgt
      181 tcgactcgag gatctcggag acgacgcgga tcgtgtaggg gaagcgctcc ttggagggga
      241 ggaccttctc tatcgagcgg cgcgcgaggt tgccgtggcc gatctcgcgg cggccggtgc
      301 cgaggcggcc cgtctcgccc acggagaagg gcgggaagtt gtagtggagc atgaagcgct
      361 cgcgcctgtc gccctcgatg tcgtcgaaga tctgctcgtc gaagacggtg ccgagggtgg
      421 tgaccgcgag ggcctgggtc tcgccgcggg tgaagagggc ggagccgtgg gtccggggga
      481 ggacgttgat ctcgcaggtg atgggcctga tgtcctcgag gccgcggccg tcgacgcgct
      541 tgccctcgtt gaggatgccg cccctgagga tctcgtacat gaggtcctcc atgagggcgc
      601 cgaagagctt cttctggacc tcgtccgtga gggcgtcggc gaacttggcc ttcgcgtcgg
      661 cgatcgcggc ggagaccgcg gcctgccgct ccatcttctc cttggtgaag agggccttcg
      721 taaggacggg gcgggcgtag gactcgatct cggccttgcg gttcaggacg accttgagcg
      781 gggcgagggg gagcttcttc ttgccgcaga ggcggccgag ctcctcgatc atcgcgcaga
      841 tctcggcgat gggcttcttc gcgacctcga tggcgccgag catctcctcc tcggagacct
      901 cgagggagtc gccctcgacc atcgtgatgc cgtgcttcgt gccggcgacg acgatctcga
      961 gcttggcctt ggcgatctgg tcgtaggtcg ggttcacgac gtaggcgccg tccaggaggc
     1021 agacccggac gccggcgatg gggccggcga aggggatgtc ggagatgtgg acggcggcgc
     1081 tggccgcgtt gatcgcgagg atgtcgggcg ggttgacgga gtcggtggag atgcaggtcg
     1141 ggacgacctg gatctcgcgg ccgaaggcct tgtcgaagag cggccgcatc ggccggtcga
     1201 tgatgcggga gacgaggatc tcgcggtcct tgggcctcgt ctcgcgcttg atgaagccgc
     1261 cggggatctt gcccgcggcg tagtatttct cgttgtactc cacggtgagg gggacgaagt
     1321 cgagtccctc ctgggcggtg gagccgcagc acgcggtcgc gatgacggcg gagccgccga
     1381 gctgggcgaa gacggatccg ttcgcctgct tggccatgcg gccggtctcg aggatgagct
     1441 cctcgccgcc cgccttgtac gacagtttct gtaccatagc taccttctat aggccaacac
     1501 gaaaggcccg aactcgtaag cgagtccggg cccccggggt ttgttcccgt cggtcccgga
     1561 ggcgattctt tccgggcgcg cggcttaagg acgcgcccgg aggctccgga ccggggaatt
     1621 acttgcggat attgagcttc tcgatgaggg cgcggtagcc cgcgagatcg tggcgctgca
     1681 tgtacttgag gagcctgcgg cgctggccga cgagcatgag gagcccgcgg cgggaattgg
     1741 tgtccttctt gttggtcttg aagtggtcgg tgagaccgtt gatccgctcc gtgaggaggg
     1801 ccacctgggt ctcggtggaa cccgtgttct tctcgctctt gccgtactcg agcacgacgc
     1861 gcgccttgtc ctctttggtg agagccattt cctttcactc cttatgtcta cagttccttg
     1921 gtaagcttta tgaaagcgac ggattcgggc ctctggcccg ccgccgtacc gaacaaaacc
     1981 cttccctcgc cgagctcgag gatggtctcc gcccggagac cggagcccct tgcctcggcc
     2041 acataacgtc cctccggcgg cgccgccctt cccgccggca gcaggaagga ggcgaagccg
     2101 tcgcccgact ccagcgcctc ggccctgcgc aggtcgagct cgtagggccg gcccagcatg
     2161 gccgccgcgt cctcgagcct gccgtccagg acggccgacc gcacgcggct cgagctcacc
     2221 gggtggccgt tccactcgag cggctcgacg atctccgcgg cgaccccgcg cgaggagtag
     2281 aactccacga tggccgcggc gtcggtgtcg agccggtgcc cgcagcggaa attcgagccc
     2341 accgcgaggt aggaaagacc gcctcgatct ctaagcaggg ataggaaatc ccttcccgcc
     2401 agtttactga aattcccgga aaagtcaatg aggacggcga gttccaggcc cagggactcg
     2461 aagccctcga gcttctgctc caggctggcc agatccccgt ggtagctcga ggggcggagg
     2521 atgcgcttcg ggttctccgc gaaggtgaag accgccggcg tcgcgcgcgg cgacctcgcg
     2581 agcacgcggc ggatgaggct ccggtgcccg aggtggagtc cgtcgaagac gccgaccgtc
     2641 gccgcgaggg gacgctcagg cctccaggtt tccctgaatt cctcccagga gacgaccttc
     2701 acgcctcgcc tccaagtacc atgcggtact cgaggctttc ggccgcctcg cggacgaggc
     2761 cgaggaaggc gccgtcgccc gagaagacgg ctgagaggga ctcgcccgcc gccgagaccg
     2821 ccgacgttcc ggcgcccgcc gccgcggcct ggttttcggc ggccgccgac gttccggcgc
     2881 ccgccgccgc ttcgtccgcc gcggccgagc cccgaaccgc cgccctggcg cccgccgcga
     2941 cgccctcgat gtccgagagg cagcgcgggc tcagtcggcc gccgttctgg aaggcgcggc
     3001 gggccggatc ctccaggcgg cggggccgga ggcccagggc cgccgccagg gccggatcga
     3061 ggaggcggag gtcgcggccc ggctcgaaat cttcggggag gaccgcctcg gagaggagta
     3121 tggggccgat ggccccgcgc tcgagggcgg ccaggctcgc gcgcgagccg gccgcgaggg
     3181 ccaggtccct ggcgagggag cggatgtagg tgccggagga gcagcggacg gagagcctcg
     3241 cgtcgcggcc gtcgtaggac aggagctcga gctcgtggat gacgacgggc cgcggggcca
     3301 tctcgacctc ctcgcccgcg aggacgcgct cgtaggcgcg cttcccgtcc aggtggatgg
     3361 ccgaataggc ggggggggcc tggaggatgg gtccgcggaa cgcggggagg acggactcga
     3421 gcttggcgag cgagggcgga ggcgcctcgg cgacgacctc gccctcgggg tcgagggtgg
     3481 aggtctcgct cccgaagcgg accagggccg agtagcgctt gtcgagggcg gtgaaatagg
     3541 gcgcgaggcg ggaatagccg ccgacgagga cgacaagtag gccggtggcg aagcggtcca
     3601 gggtgccggc gtggccgacc ttgcccgtcc ccaggccccg cttgaggggc cccagggcgg
     3661 cgaaggaggt caggccggcc cgcttctcga tggcggcgag gccggagagt cgagtcacgt
     3721 gaaaaggccc ttgatcctct cgttgagctc gaagccctct ttgatgcccc ggtcggccac
     3781 gaagacgagc ttcggggtga gccgggtgcg gagccgcctg ccaacctggg cctggatgta
     3841 gcccgaggcc cgggacaggc cctccaccgc gaggtccagg cgctgggcct cggcctccgc
     3901 ctgggactcc gcgtcgcccc cggcctcgcc gcccgaggcg aggccgaaga ccgagacgta
     3961 gaccttggcc gcggagaggt cccgcgcggc ctcgacccgg gagaccgaga cgaaggcgcc
     4021 gacgcgggga tccttgacct cgcccgagag gatgagggag gctatctccc cccggagctg
     4081 ctcctcgagc ttcttatgcc tgatctcgtc cattggcctt cccagcgtcc gccggggcgg
     4141 agagcgtgcg ggcgatctcg atcatctcgt agatctcgag ggtgtccccg gccttcacgt
     4201 cctggaggcc ctcgatgccg atgccgcact cgtagccggc cgcgacttcc ttcgcgtcgt
     4261 ccttgaagcg cttgagggag ttgatcttgc cctggtggat ctccacgcct tcccggatga
     4321 cgcgcacctg gcagttgcgg cgcacgcagc cctcgaggac gaagcagccg gcgacgacgc
     4381 cgaccttcgg cacctggaag gtctcgcgga cctccacctg gccgatctcg ctctccttga
     4441 gctcgggggc gagcatgccc tccatggctt tcttgatctc ttcctcggcg cggtagatga
     4501 tgttgtactt gcggatgtcg accttctcgc ggtcggcgag gagcttggcc gcggggctgg
     4561 ggcggacgtt gaaggcgatg atgatcgcct cggaggcggc cgccatgttg acgtcgtcct
     4621 cggagatggt gcccgccgcg gagcggatga cgttgagcct gacctcgggc gtcgagagct
     4681 tctccaggag gcccttgagg gcctcgacgg agccctgcac gtcgcccttg atgatgacct
     4741 tgagctcctg cacgtcgccc tgctggatgg tcgagtagag attgtccagc gtcaccttct
     4801 tgacgttctt ggactcctcg tactttttga gctcctgccg cttcatcgag atctggcggg
     4861 cgtacttctc gtcctccacg acctcgaagg ggtcgccggc gttcggcagg ccctcgaagc
     4921 cgatgacctc gacgggcatc gacggggtgg cctcctcgat cctctcgccc ttgtcgttga
     4981 agagggcgcg gaccttgccg gagtagatgc cggcggcgaa ggagtcgccg atgcgcaggg
     5041 tgccgcgctc cacgatgacc gtggacacga tgccgcggcc ctggtcgacg cgggactcga
     5101 cgaccttggc ctcggcccgg ctgtcgtagt tggcggtgag ctcgagcacc tcggcctgga
     5161 ggaggatggc gtccaggagc tcggggatgc cgcgcttctg gagggcggag atctggcaga
     5221 agatggtcgt gccgccccag gcctcggggg tgaggccgag ctccgcgagc tgcgacatga
     5281 cgcggtcggg attggcctcg gggaggtcga tcttgttgac ggcgacgatg atggggaggt
     5341 tggccgcctt cgcgtggtcg atggcctcgc gggtctgggg catcacgccg tcgtcggccg
     5401 cgaccaccag gacgacgatg tccgtcacct gggcgccgcg ggcgcgcatc ttcgtgaagg
     5461 ccgcgtggcc cggggtgtcg aggaaggtga tctttccctt cggcgtctcc acctggtagg
     5521 cgccgatgtg ctgcgtgatg ccgccgaact cgccggcgac cacgtcggtc ttcctgatcg
     5581 cgtccaggag cttcgtcttt ccgtggtcga cgtggcccat gacggtgacg acgggaggcc
     5641 ggtgctgcag ctcgccgggc aggtccgcca ccttctcgat gacggtctcg tcgtagaggg
     5701 agacgatgtg cacctcgcag ccgtactcgc cggccaggag ggtcgcggtg tcggagtcga
     5761 tcttctggtt gatcgtcgcc atgacgccga gcttcatgag gttggcgatg aggtcggccg
     5821 gcttcaggtt catcttcttg gccaggtcgg agacggagat ggtctccatg atgtcgatct
     5881 ccttggggat cgccatcgcc tgggcttcct gcttcttctt gagcctgagc gccttctcgg
     5941 cctcttcctc gcgcttggtg taggcgggct tcttgcctcg gacgggcttg cggaccgcgg
     6001 tcttgccggt tccggcgccc atggaggagc cgccggggcc gccgggcctg ccgggaccgc
     6061 caggtcgctg gccgtagccg ccgggcctgc cgggcccgcc gggacgctgt ccgtagccgc
     6121 cctggccgta gcctccgggg cgctggccgc cgtagccgcc gccctggccg tagccggggc
     6181 gcgggccgcc gtagccgccg cctgagggtc cgccctggcc gtagccgggg cgcgggccgc
     6241 cgtagccgcc gccctgagga c
//
LOCUS       DAOOSJ010000165         6256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7739_length_6256_cov_5.220771, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000165 DAOOSJ010000000
VERSION     DAOOSJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7739_length_6256_cov_5.220771"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..491
                     /locus_tag="PLG14_08690"
     CDS             186..491
                     /locus_tag="PLG14_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX49246.1"
                     /translation="MRFLVIDDSATMRKIVGLALTGAGHTYSEAENGQMGLELAKGST
                     FDCIMLDVNMPVMNGLEFLGQRAADAAIRKIPVVVLTTQSEESLKAEAKRLGAEAFL"
     gene            complement(670..1821)
                     /locus_tag="PLG14_08695"
     CDS             complement(670..1821)
                     /locus_tag="PLG14_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49247.1"
                     /translation="MSPSPEPLLREALAALAEASRAAIEEAAKGRDPGPAAAALGRLA
                     AAAAGDSPEELLLAAKTLPWALPASARIKEAGEGLSEARALAACAAEVIAAVPRSTEA
                     ATFAIIERFEAVRSMTSRAAEAARGARGLFSGEEGSSLLSTAKGSREAVVSERLAVAE
                     IAARNKASAKALREIHKAVDDGIELLRGIEDITERSRLIAFNLAVEAAHFGDKGRGFK
                     IIAGELRSLNDRTEDFSGKVTGLLERLGSESADLERETAEETDRMVSEAEKGMGATER
                     AVESLIGASQETERFAREIAALAERIDADMSGVLESLQFQDITRQVVEGAAAMLGELP
                     PILAAARSALPELDRGDSLDAERFEAVRSRLIAAAKTKDEKEALAKARP"
     gene            1973..2602
                     /locus_tag="PLG14_08700"
     CDS             1973..2602
                     /locus_tag="PLG14_08700"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOX49248.1"
                     /translation="MEALLAIGSEAPPRGRLASRLDGFGLVCAADSGLDLLAAWGVEP
                     DLVVGDMDSLSSLDLLAAYPDAEILRFPRAKDESDTELGLRILHERGASRVVIAGGGG
                     GRIDHLLAIRALFERPRPPAEWHAARESIYLVAEGRRLEFLAAEGETVSVFPLSSGAE
                     GMRSSGLRWPLDGLRWGPGDFGLSNEAAGGGVRVEAGRGSLLVVREMRD"
     gene            complement(2690..3166)
                     /locus_tag="PLG14_08705"
     CDS             complement(2690..3166)
                     /locus_tag="PLG14_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOX49249.1"
                     /translation="MEYFFNFDKISYLKGKRPDGCILCLIRDGSAEVERLVAAETASF
                     VVSVNLYPYNPGHLILFPKRHVVDIRELSPGERLELDRLVDRCLAALDESHGPAGYNI
                     GYNMGLVAGASIDHLHLHIIPRYPREIGISELIAGARVLVEDPRNTLVRFKEAFKA"
     gene            3227..3679
                     /locus_tag="PLG14_08710"
     CDS             3227..3679
                     /locus_tag="PLG14_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49250.1"
                     /translation="MELVEGRSEEGAAAPGASPAEAAAAPRGAAVPASGGRNPSVAAE
                     APYALRVLKRLAAFLLALALIALFFWAAGNFRLFLDDTQLMLLSVLRWSSLGLGVLSL
                     VGAAASLARQGMLAGRLRSAGGWLLLVLSGTALALLSEALIVFAAGLR"
     gene            3701..3913
                     /locus_tag="PLG14_08715"
     CDS             3701..3913
                     /locus_tag="PLG14_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOX49251.1"
                     /translation="MDEALARIEALELKVSYQEATITELSRALYEHDQRLERLEEFSR
                     EASSKLKEAAEAGLPPLPADERPPHY"
     gene            complement(4157..4921)
                     /locus_tag="PLG14_08720"
     CDS             complement(4157..4921)
                     /locus_tag="PLG14_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOX49252.1"
                     /translation="MMRSFVRATLALGAASVFGLCLVLGAGAQAAQPATAPKSGEKTV
                     EEAYLQESLETMIIKEQSQSDSKDMKLVALQYIKQAVDAGRKNEEIRKSLEYLALETT
                     MVVTRSAGLGRPTNDYPDIRAKACDLLGEFPSVESKNALLKVALGDAEPMVLSAAVRS
                     LGKIGMNDGDDVTQTIAFIVNRFDILMPDNSLAFESLIAIERIADKNNGIKDPAAIRA
                     VMKIATGNYITPVRAKANEILGKLRKYSAGSGGSGR"
     gene            complement(4921..5493)
                     /locus_tag="PLG14_08725"
     CDS             complement(4921..5493)
                     /locus_tag="PLG14_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49253.1"
                     /translation="MTSRASEKQSLAGAFIASALLALLAAGCAAAPAAPSTPPPVAPA
                     TSPPAAPAQPPAASFDPAAVSVEVKTAAIADIKALIEGLNTIIQRKDYESWLGYLTEP
                     YKLHYSDPAVLAQISEYPVLKRAGIKLSSLRDYFLYVVYPSRQNDRVDDIEYLEEGLV
                     KAITVSPKGDRQILYNLEKHGDTWKIGIGR"
     gene            5672..>6256
                     /locus_tag="PLG14_08730"
     CDS             5672..>6256
                     /locus_tag="PLG14_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOX49254.1"
                     /translation="MKDIVALHRAEYEGPPEVVVAAPGVVKFLGEQVEASESLVLAAA
                     VSFEIRVAVSLRRDSSLRFFAADFGERKRTSVGNLKFKREDRWANYPKAVLDLLLREG
                     LPTKGLNFTIAGDIPVGVGLASSNALVLASALAVKELYALSRRPEELSELGRRAELEF
                     LGRASSLSDSLTASMSTPGSVSIVDLRSARRRALP"
BASE COUNT          879 a         2111 c         2206 g         1060 t
ORIGIN      
        1 caagcgaccg tactccccaa gaggcctccg gtccgcgggc gcccctcgcc atcctcgcca
       61 tcctcgccat cctcgccagc ccgcaaggcc gattccttgt cgccggcaaa tcggggcgct
      121 atactgagtt cactggcgcg cgataaaaat tgattgaaag ccgtccagac gacgaggatg
      181 atgccttgcg ttttctcgta atcgacgatt cggcgaccat gaggaagatc gtgggcctgg
      241 ccctcacggg cgcgggccat acctattcgg aggccgagaa cggccagatg ggcttggagt
      301 tggccaaggg cagtaccttc gactgcatca tgctcgacgt gaacatgccc gtgatgaacg
      361 gcctcgagtt cctgggccag cgcgcggcgg acgcggcgat ccgcaagata ccggtcgtcg
      421 tgctcaccac ccagagcgaa gagagcctga aggccgaggc caaaaggctc ggggccgagg
      481 ccttcctgta gaagcccttc cagaaggagg ccctcctcaa ggtggtctcc gccctcgcgg
      541 ccaagtaggc gcatacgcga ggaggccgcg cgggatgtcc cgcgcggcct ccttgagcgg
      601 cctatgaagc cagctccctg ggccgtccct tgggcagagc cacgtaggcc gccccgcttg
      661 ggcggctctc tagggtctcg ccttcgccaa ggcttccttc tcgtccttgg tcttcgcggc
      721 ggcgatgagt cgggacctga cggcctcgaa gcgctcggcg tccaggctgt cgcccctgtc
      781 gagctcgggg agggcgcttc tggccgcggc caggatgggc ggcagctcgc caagcatggc
      841 ggctgcgcct tccacgacct gcctcgtgat gtcctggaac tgcagcgact cgaggacgcc
      901 gctcatgtcg gcgtctatcc gctcggcgag cgccgcgatc tcccgggcga agcgctcggt
      961 ctcctgcgag gcgccgatga gcgactcgac cgcccgctcc gtcgctccca tgcccttctc
     1021 ggcctcggac accatccggt ccgtctcctc cgcggtctcg cgctccaggt cggcgctctc
     1081 cgagcccagg cgctcgagga ggcccgtgac cttgccggag aaatcctccg tgcggtcgtt
     1141 gagggacctg agctcgcccg cgatgatctt gaagccccgg cccttgtccc cgaagtgcgc
     1201 ggcctcgacg gccaggttga aggcgatgag gcgggagcgc tccgtgatgt cctcgatgcc
     1261 tcggaggagc tcgatgccgt cgtccaccgc cttgtggatc tcgcggaggg ccttggcgct
     1321 cgccttgttg cgtgcggcga tctccgccac cgcgagccgc tcggagacga cggcctccct
     1381 ggaacctttg gccgtggaga ggaggctcga gccctcctct cctgagaaga gtccccgggc
     1441 tccccgcgcg gcctcggccg cccgcgaggt catggacctg acagcctcga aacgctcgat
     1501 gatggcgaag gtcgccgcct cggtggaccg gggcacggcc gcgatgactt ccgcggcgca
     1561 ggcagccagg gccctggcct cggacagccc ctcgcccgcc tccttgatcc tcgccgaggc
     1621 gggcagggcc cagggcaggg tcttggccgc gaggaggagc tcctcggggc tgtcgcccgc
     1681 cgccgccgcg gccaggcgcc cgagggcggc ggccgccggg cccgggtcgc ggcccttcgc
     1741 cgcctcctcg atcgcggcgc gggaggcctc ggctagggcg gccagcgcct cgcggaggag
     1801 cggctcgggg gaagggctca tggctggagc gtcggccggc cccctggaga gggggccgcg
     1861 gggggccggg cggtcttggg aacgcatgcc tgaaatctag agcagagccg cccgtcctgc
     1921 aatagcggcg gcccttgaca gggcgggggg ccgcggtcaa actccccgcg tcatggaagc
     1981 cctgctcgcg atcggttcgg aggccccgcc gcgcgggcgg ctcgcctcgc gcctggacgg
     2041 cttcggcctc gtctgcgccg ctgactcggg cctcgacctc ctggccgcct ggggcgtcga
     2101 gcccgacctg gtcgtcggcg acatggacag cctctcgagc ctggacctcc tggccgccta
     2161 tcccgacgcg gagatcctgc gcttcccccg ggccaaggac gagagcgaca ccgagctcgg
     2221 cctcaggatc ctccacgagc ggggcgcctc ccgcgtcgtc atagccgggg gcgggggagg
     2281 gcgcatcgac catctcctcg cgatccgcgc cctcttcgag cgtccgcggc cccccgcgga
     2341 atggcacgcc gcccgcgagt cgatctatct cgtggccgag ggaaggcgcc tcgagttcct
     2401 ggcggccgag ggcgagaccg tgtcggtctt ccccctttcc tccggcgcgg agggcatgag
     2461 gagctcgggc ctgcgctggc ccctggacgg cctacgctgg ggtccggggg acttcggcct
     2521 gagcaacgag gcggccgggg gaggcgtgcg cgtggaggcg gggaggggaa gcctcctcgt
     2581 cgtgcgggag atgcgggact gagggccgcc gcgcttgcgt aggcgcggcg acgcggcgcg
     2641 aaacggccgc cggccggagc gcggcgcccg agggcgccca gccgggctcc taggccttga
     2701 aggcctcctt gaagcggacg agggtgttcc ggggatcctc caccaggacc ctcgcgccgg
     2761 cgatgagctc ggagatgccg atctcgcgcg ggtagcgcgg tatgatgtgg agatgcaggt
     2821 ggtcgatgct ggcgcccgcg accaggccca tgttgtagcc gatgttgtag ccggccggcc
     2881 cgtggctctc gtccagggcg gctaggcagc ggtccaccag gcggtccagc tccagccgct
     2941 cgccgggaga gagctcgcgg atgtcgacga catggcgctt ggggaagagg atgaggtgcc
     3001 cgggattgta gggatagagg ttgaccgaca ccacgaagct cgcggtctcc gcggccacga
     3061 ggcgctccac ctccgccgag ccgtcgcgga tgaggcagag tatgcagccg tccgggcgct
     3121 tgcccttgag gtaggagatc ttgtcgaaat tgaagaagta ctccatggaa tcccttcgcc
     3181 ttgcggccca agccgatcct agcctagaat ggcgaaaggc gccagtatgg aactagtcga
     3241 gggcaggtcg gaggagggcg cggccgcgcc gggcgcgtcg cccgctgagg ccgccgcagc
     3301 gccgcgcggg gcggctgtcc cggcgtcggg aggccggaac ccgtccgtcg ccgccgaggc
     3361 gccctatgcc ctgcgcgtcc tcaagcggct cgcggccttc ctcctcgccc tcgccctcat
     3421 agccctcttt ttctgggcgg cgggcaattt caggctcttc ctggacgaca cccagctcat
     3481 gctcctctcg gtcctgcgct ggtcctccct cggcctcggg gtcctctccc tggtgggggc
     3541 ggccgcgagc ctggcacggc aggggatgct cgccggccgc ctgcgctccg ccgggggctg
     3601 gctcctcctc gtcctttcgg gaaccgcctt ggccctcctg tcggaggccc tcatcgtatt
     3661 cgccgccgga ttgcgctaag gagagatcat gcttgattcc atggacgagg ccctcgcgag
     3721 gatcgaggcg ctcgagctga aggtctccta ccaggaggcg acgatcacgg agctgtcccg
     3781 cgcgctgtac gagcacgacc agcgccttga aaggcttgag gaattctcga gggaggcctc
     3841 gtcgaagctg aaagaggcgg cggaggcggg cttgccgccg ctgcccgcgg acgagaggcc
     3901 tccccactac taggcgtagg tgtccacgct ttcgcccgtt cccggcgccg gttcgggcgg
     3961 tgcctccact ggagcctccg gctcgggagc ctctgcctgg ggctggggcg gggccggaag
     4021 gggcgaaagc agcggatcca gcacgtttcc gatgccgtcc atgtccgcct cctaccctga
     4081 atttaagctt tttgcggaaa aaaagcgaac cctcccggcc gggagggttc gcgctttctc
     4141 gagggcttaa gggaggctac ctgccggagc cgcccgagcc cgcggagtat ttcctgagct
     4201 tcccgaggat ctcgttggcc ttggcgcgga cgggggtgat gtagttgccg gtggcgatct
     4261 tcatcacggc ccggatggcg gcggggtcct tgatgccgtt gttcttgtcg gcgatgcgct
     4321 cgatggcgat gaggctctcg aaggcgaggc tgttgtccgg catgaggatg tcgaagcggt
     4381 tgacgatgaa ggcgatggtc tgggtgacgt cgtcgccgtc gttcatgccg atcttgccca
     4441 gggagcggac cgcggcggag agcaccatcg gctcggcgtc gcccagggcc accttgagga
     4501 gggcgttctt ggactcgact gaggggaact ccccgaggag gtcgcaggcc ttggcgcgga
     4561 tgtccggata gtcgttcgtc ggcctgccca ggcccgccga gcgggtcacg accatcgtcg
     4621 tctccagggc gaggtactcg agggacttgc ggatctcctc gttcttcctg ccggcgtcga
     4681 ccgcctgctt gatgtactgg agggccacga gcttcatgtc cttcgagtcg gactgggact
     4741 gctccttgat gatcatcgtc tcgagggatt cctggagata ggcctcctcg acggtctttt
     4801 caccgctctt gggggcggtc gcgggctggg ccgcttgggc cccggcgcct agtacgagac
     4861 agagcccgaa caccgaggcg gcgccgaggg cgagggtcgc gcggacgaag gaacgcatca
     4921 ttacctcccg attcctatct tccaagtatc gccatgcttc tcgagattat agagtatttg
     4981 ccgatcgccc ttggggctca cggtgatggc cttgacgagg ccctcctcca ggtactcgat
     5041 gtcgtccacc ctgtcgttct gcctggaggg gtagacgacg tagaggaagt agtcccgcaa
     5101 ggaggagagc ttgatccccg cgcgcttgag cacgggatac tcggagatct gcgcgaggac
     5161 ggcgggatcc gagtagtgga gcttgtaggg ctcggtcaga tagccgagcc aggactcgta
     5221 gtccttcctc tggatgatcg tgttgagtcc ctcgatcagg gccttgatgt cggctattgc
     5281 ggccgtcttg acctcgacgc tgacggcggc gggatcgaag gaggccgcgg gcggttgggc
     5341 cggcgcggcg ggcggagacg tcgcgggcgc gacgggcgga ggggtcgaag gcgccgccgg
     5401 cgcggcggcg cagcccgcgg cgaggagggc gaggagggcc gacgcgatga aagcccccgc
     5461 aagggactgt ttttcggatg cgcggctggt catggcctta tcatatagcc tcgggcgagg
     5521 aatgtcaaaa gcgcatcgct tccgggcggt cgatttttat tgaagggccg ggccggatag
     5581 tgtatcatgc catcattcat cgaattggcg gagggattcc gcggggagga gctttccgat
     5641 ggctcccgcg gggcggccgg atgaggtact aatgaaggac atcgttgcgc tccaccgcgc
     5701 cgagtacgaa gggccgccgg aggtcgtcgt ggcggccccg ggagtcgtca aattcctcgg
     5761 cgagcaggtc gaggcctccg agtcgctcgt cctcgccgcg gcggtctcct tcgagatccg
     5821 ggtcgccgtc tccctccgcc gcgattcctc cctgcgcttc ttcgccgccg acttcggcga
     5881 gcgcaagcgc accagcgtcg gaaacctcaa gttcaagcgg gaggaccgct gggcgaatta
     5941 cccgaaagcg gtcctggacc tccttctccg cgaagggctt ccgaccaagg ggctcaactt
     6001 caccatcgcc ggcgacatcc ccgtcggcgt cggcctggcc tcctccaacg ccctcgtcct
     6061 cgcctcggcc ctggccgtga aggagctcta cgccctgtcc cgccgccccg aggagctctc
     6121 cgagctcggg cgccgggccg agctcgagtt cctgggccgc gcctccagcc tctccgactc
     6181 cctgaccgcc tccatgtcca cgcccggctc ggtgtccatc gtcgacctcc gctccgcccg
     6241 ccgccgcgcc cttccc
//
LOCUS       DAOOSJ010000166         6229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7791_length_6229_cov_5.672660, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000166 DAOOSJ010000000
VERSION     DAOOSJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7791_length_6229_cov_5.672660"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /gene="htpG"
                     /locus_tag="PLG14_08735"
     CDS             complement(<1..1892)
                     /gene="htpG"
                     /locus_tag="PLG14_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOX49255.1"
                     /translation="MPKHHFKTEVSRLLDLIIHSLYSNKEIFLRELVSNASDALDKLS
                     YLRVSDEAYKTLALEPRIEVRLDKEAKILSVLDNGVGMDEKELEDNLGTIARSGTRKF
                     LEALPQEAKKDSNLIGQFGVGFYSAFMVADRVEVTSRRAGKPEAHRWTSDGKGEYSIE
                     AVPAFDAPGSAPTAQGTEVRLYLNDEGEELLSRWKAEDILKKYSNHVAYPIHLVSMEA
                     EYEKGEKKGETRKDEQVNAASALWRRPKAELKDEDYKEFYKGFAAEHLGFGAADDEPL
                     LWMHTRAEGTIEYTSLFFVPKKAPADLYRADYRPGVKLYVRRVFITDDEKELLPVYLR
                     FVRGVIDSEDLPLNVSREILQQNRVMNQIRTGSVKKILGELAELSKADPEKYAAFVAE
                     FNRPLKEGLYSDYANREALLGLVRWKSTRSEAGASGAAAWTSLADYKARMKDGQKAIY
                     YIAGGEEERLRKSPLLEAYRKEGIEVLVCPDEIDEIVLGGIGRFEELEFKAVNRAGSE
                     EGLESAKAAETGEEAGKAAEKAKAALGAAVKDVRLSKRLAESPACVVLDGDEPSLQLR
                     QLLKEMGGSELPEMKPVLELNGSHALVKRLAAAEGPEAEDLARVLLGQALLVEGLPLE
                     DPVDFA"
     assembly_gap    1964..2063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2064..2908)
                     /locus_tag="PLG14_08740"
     CDS             complement(<2064..2908)
                     /locus_tag="PLG14_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX49256.1"
                     /translation="MDPKGPAGAATASAAPAPRLELRTAGELALTFIGAGSAFSKKFY
                     QNNLLIAKGEDHLLVDCGTRAPEALASLGLSVTDIGAFLITHSHADHIGGLEEVMLLN
                     RYAARKKAKIVVTRKLEKLLWAMSLRGGASYNEVHGGKPLEFGDFWEPIHPKRVRGAD
                     RELSVAVLGGIEVALFRTKHIPDSAAGWQDSFPSYGLVIDRRVLFTSDSRYDPEMVLA
                     MDEAYGLEAIFHDCQLFKGGVHAALEEIAGLPPKIKAKTRVMHYGDKIEDFRGRVAEL
                     GFAGVA"
     gene            3099..4112
                     /locus_tag="PLG14_08745"
     CDS             3099..4112
                     /locus_tag="PLG14_08745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX49257.1"
                     /translation="MENALYSRVSARMGTAFRSLSAFAGRVGKAGKQKLTIMFIPHTE
                     KSIFNVQLSFFALAGIVLAFGVVLFSSIFFTARFSDTHRRLQSRSEDLKSSQADLDAI
                     RDETSRLVTAAKRFETALSGTLARVGVRQSSPEAAAQRGDLASFFETDEAGTGGVREI
                     GEIKKVTDYLEKSVDPMKELGALLENQSAVLTEIPNIWPIKGGIGHISMYYGQNENPF
                     TGQWYIHKGIDISTFRTGDPIVATADGKVVAVGFDSGFGNYVILQHSHGFFTRYAHMQ
                     SFRVYKGQKVQQGQVIGYIGNTGLSTGPHCHYEVHLGTGVIDPLKFLNIRASAVAVSD
                     KDK"
     gene            4125..4646
                     /locus_tag="PLG14_08750"
     CDS             4125..4646
                     /locus_tag="PLG14_08750"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOX49258.1"
                     /translation="MPDIKDSLVNSLIGAGSAVDGDLDIEGMLRVDGDLRGSVRATGK
                     VVVGASGRVEASIRARSAIIGGLVKGDVYVTEQLRILSGGVVVGNVFAPRLEAEDGTL
                     VHGDLAVTGRPDRAEDELKAFVERYGDAARFLCRFRSSDFAAQAARAAGGRDSRRTAA
                     AVSGGESGGPGQA"
     gene            4648..5154
                     /locus_tag="PLG14_08755"
     CDS             4648..5154
                     /locus_tag="PLG14_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOX49259.1"
                     /translation="MAKIDFPEGLPNPFAPMMLPKRREDEKKAGKSKGLFRTSLGKAL
                     DEARAGSGSGGLASEPFSEAALESLLDEVHEAGDRLKENANVDAVQAYKKSVRDFVHY
                     VVEKSYSVEERTSGGSILKRKKFYLVAVIDESLERLAAEILRNQRDKLEILRRVDEIN
                     GMLVDLLR"
     gene            5167..6228
                     /gene="ricT"
                     /locus_tag="PLG14_08760"
     CDS             5167..6228
                     /gene="ricT"
                     /locus_tag="PLG14_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOX49260.1"
                     /translation="MADFSDEDTSEEALKGLEDEGLAGGQAEAEAAAEGEGDAESQAE
                     GEGREGGGALSGQRERSRGEQRGRGRELPSPLYRIRATHSNETFFAWWEGEGELAPRT
                     WVVAPTRYGRDVCELQGLVRCPGHVGADEILKIDRIATEEDLSKVKANEERERKAFEL
                     CRQKIETRRLPMKLVAAHYLFDEPKVLFFFSAESRVDFRELVKDLVSVFKMRIELRQI
                     GVRDEARVTGGCGVCGRVLCCHGVTDKLTPVSIKMAKDQNLSLNSMKISGPCGRLLCC
                     LAYEYGFYRDARRELPGEGVRFLFEGVNFRVVEVNALAGSVKAAGEDGRVLWMQAARF
                     IHSEGKWKVREEIPDPDRA"
BASE COUNT          952 a         2034 c         2140 g         1003 t
ORIGIN      
        1 tgggcgaagt ccacgggatc ctcgaggggc aggccttcga cgaggagggc ctggccgaga
       61 aggacgcggg ccaggtcctc cgcctcgggg ccttcggccg cggccaggcg cttcacgagg
      121 gcgtgggagc cgttgagctc caggacgggc ttcatctcgg ggagctccga gccgcccatc
      181 tccttcagga gctggcggag ctggaggctc ggctcgtcgc cgtccaggac gacgcaagcc
      241 gggctctccg cgaggcgctt ggagaggcgg acgtccttca ccgcggcgcc cagggcggcc
      301 ttggccttct ccgcggcctt gccggcctcc tcgccggtct ccgcggcctt cgcggactca
      361 aggccctcct cgctgcccgc gcggttcacg gccttgaact cgagctcctc gaacctgcct
      421 atgccgccga ggacgatctc gtcgatctcg tcggggcaga ccaggacctc gatgccctcc
      481 ttgcggtagg cctcgaggag gggggacttg cggaggcgct cctcctcgcc gcctgcgatg
      541 tagtagatgg ccttctggcc atccttcatg cgcgccttgt agtcggccag gctcgtccag
      601 gcggccgcgc ctgaggcgcc ggcctcggag cgggtggact tccaccggac caggcccagg
      661 agggcctcgc ggttcgcgta gtccgagtag aggccctcct tgagggggcg gttgaactcg
      721 gcgacgaagg cggcgtactt ctcgggatcg gccttggaga gctccgcgag ctcgcccagg
      781 atcttcttca cgctgcccgt ccggatctgg ttcatcacgc ggttctgctg caggatctcg
      841 cggctcacgt tgagcgggag gtcctcggag tcgatgacgc cgcggacgaa gcggaggtag
      901 acggggagga gctccttctc gtcgtcggtg atgaagacgc ggcgcacgta gagcttgacg
      961 ccggggcggt agtcggcgcg gtagaggtcg gcgggcgcct tcttcgggac gaagaagagg
     1021 ctcgtgtact cgatggtgcc ctccgcgcgg gtgtgcatcc acaggagggg ctcgtcgtcg
     1081 gccgcgccga agcccaggtg ctcggcggcg aagcccttgt agaactcctt gtagtcctcg
     1141 tccttgagct cggccttggg gcgccgccag agggcgctcg cggcgttgac ctgttcgtcc
     1201 ttcctcgtct cgcccttctt ctcccccttc tcgtactcgg cctccatgga gacgaggtgg
     1261 atggggtagg cgacgtggtt cgagtacttc ttgaggatgt cctccgcctt ccagcgggag
     1321 aggagctcct cgccctcgtc gttgaggtag aggcggacct ccgtgccctg ggcggtcggc
     1381 gcggagccgg gggcgtcgaa ggcgggcacg gcctctatcg agtactcgcc cttgccgtcg
     1441 ctcgtccagc ggtgcgcctc gggcttcccg gcgcggcggc tcgtcacctc cacgcggtcg
     1501 gcgaccatga aggcggagta gaagccgacg ccgaactggc cgatgaggtt cgagtccttc
     1561 ttggcctcct ggggcagggc ctccaggaac ttgcgggtgc ccgagcgggc gatggtgccc
     1621 aggttgtcct cgagctcctt ctcgtccatg ccgacgccgt tgtccaggac ggagaggatc
     1681 ttggcctcct tgtccaggcg gacctcgatg cggggctcca gggccagggt cttgtaggcc
     1741 tcgtcggaga cgcggaggta ggagagcttg tccagggcgt cggaggcgtt ggagacgagc
     1801 tcgcggagga agatctcctt gttggagtag agggagtgga tgatgaggtc gaggaggcgc
     1861 gagacctcgg tcttgaagtg gtgcttgggc atgttcgcgt acctttcggg gattgggttg
     1921 tcttcggatt ggaaaatagc gaagggcggg ggccggaggc aagnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnngcgacgc ccgcgaagcc gagctcggcg acgcggccgc
     2101 ggaagtcctc gatcttgtcc ccgtagtgca tcacgcgggt cttggccttg atctttggcg
     2161 gcaggcccgc gatctcctcc agggcggcgt ggaccccgcc cttgaagagc tggcagtcgt
     2221 ggaagatcgc ctccaggcca taggcctcgt ccatcgcgag gaccatctcg gggtcgtagc
     2281 ggctgtcgct cgtgaagagg acgcggcggt cgatgacgag gccgtagctc gggaaggagt
     2341 cctgccagcc cgcggcggag tcggggatgt gcttggtgcg gaagagggcg acctcgatgc
     2401 cgcccaggac ggcgacggag agctcgcggt cggccccgcg cacgcgcttc gggtggatcg
     2461 gctcccagaa gtcgccgaat tcgaggggct tcccgccgtg gacctcgttg taggaggccc
     2521 cgccgcgcag ggacatggcc cagaggagct tctcgagctt ccgggtgacg acgatcttcg
     2581 ccttcttgcg ggccgcgtag cggttcagga gcatcacctc ctccaggccg ccgatgtggt
     2641 cggcgtggct gtgggtgatg aggaaggcgc cgatgtccgt cacggagagg ccgagcgagg
     2701 ccagggcctc gggggcgcgg gtgccgcagt cgacgaggag gtggtcctcg cccttggcta
     2761 tgaggaggtt gttctggtag aatttcttcg agaaggcgct gcccgcgcct atgaaggtca
     2821 gggccagctc gcccgccgtg cggagctcga ggcggggtgc gggggccgcg ctcgccgtgg
     2881 cggcgccggc cgggcctttc gggtccatct cctaaagtgt atcggttgga agctccgctt
     2941 tccaaagaaa atacgtcaaa tggggccttg gagcccctct tttcaggttt tcgtttgaca
     3001 ggccgataat cgagatggta gagtaggata gaagcggtat agtcccattc gtatcggtta
     3061 aggagagggt cgtgtccacc cttcgaaaat ataagaaaat ggaaaacgcc ctctactcgc
     3121 gggtttcggc ccggatgggg accgctttcc gctccctctc cgccttcgcg ggccgcgtgg
     3181 ggaaggccgg caagcagaag ctcaccatca tgttcatccc ccacaccgag aagagcatct
     3241 tcaacgtcca gctctccttc ttcgccctgg ccggcatcgt cctggccttc ggcgtggtcc
     3301 tcttctcctc catcttcttc accgcccgct tttccgacac ccaccgccgc ctccagagcc
     3361 gctcggagga cctgaagtcg agccaggccg acctggacgc catccgcgac gagacgagcc
     3421 gcctcgtgac cgccgccaag cgcttcgaga ccgccctctc cggcaccctg gcccgcgtcg
     3481 gcgtcaggca gtcgagcccc gaggcggccg cccagcgcgg cgacctggcc tccttcttcg
     3541 agacggacga ggcggggacg ggcggggtcc gcgagatcgg cgagatcaag aaggtcacgg
     3601 actacctgga gaagtcggtc gatcccatga aggagctcgg cgccctcctg gagaaccaga
     3661 gcgccgtcct cacggagatc ccgaacatct ggcccatcaa gggcggcatc ggccacatct
     3721 ccatgtacta cggccagaac gagaacccct tcaccggcca gtggtacatc cacaagggca
     3781 tcgacatctc caccttccgc acgggcgacc ccatcgtcgc caccgcggac ggcaaggtcg
     3841 tcgcggtcgg cttcgactcc ggcttcggca actacgtcat cctccagcac agccacggct
     3901 tcttcacgcg ctacgcccac atgcagtcct tccgggtcta caagggccag aaggtccagc
     3961 agggccaggt catcggctac atcggcaaca cgggcctctc caccggcccc cactgccact
     4021 acgaggtgca cctgggcacg ggcgtgatcg accccctcaa gttcctgaac atcagggcga
     4081 gcgccgtggc ggtcagcgac aaggacaagt agcccggagc gcccatgcca gatatcaagg
     4141 attccctcgt caattccctc atcggggccg gttccgccgt ggacggcgac ctcgacatcg
     4201 agggcatgct ccgcgtggac ggcgaccttc gcggctcggt ccgggccacg ggcaaggtcg
     4261 tcgtcggcgc ctccggccgc gtggaggcct ccatcagggc ccgctcggcc atcatcggcg
     4321 gcctcgtgaa gggcgacgtc tacgtcaccg agcagctccg catcctctca ggcggcgtcg
     4381 tcgtcggcaa cgtcttcgcg ccccggctgg aggccgagga cggcaccctg gtccacggcg
     4441 acctggccgt cacgggcagg cccgaccggg ccgaggacga gctcaaggcc ttcgtggagc
     4501 gctacggcga cgccgcgcgc ttcctctgcc gcttccgctc ctcggacttc gccgcccagg
     4561 cggcgcgcgc cgcgggcgga cgggactccc gcaggaccgc ggcagcggtc tcgggcgggg
     4621 agagcggcgg cccgggacag gcctaggatg gccaagatcg atttcccgga ggggctcccg
     4681 aaccccttcg cccccatgat gctcccgaag cgccgggagg acgagaagaa ggccggcaag
     4741 agcaagggcc tcttcaggac gagcctgggg aaggccctgg acgaggcgag ggcgggctcg
     4801 ggttccggcg gcctggcctc cgagcccttc tccgaggcgg ccctcgagtc cctcctggac
     4861 gaggtgcacg aggcggggga ccggctcaag gagaacgcca acgtcgacgc ggtgcaggcc
     4921 tacaagaagt cggtgcgcga cttcgtgcac tacgtcgtgg agaagtccta ctccgtggag
     4981 gagcggacct ccggcggcag catcctcaag cgcaagaaat tctacctggt cgcggtgatc
     5041 gacgagtccc ttgagcggct cgccgcggag atcctccgca accagcgcga caagctcgag
     5101 atactgaggc gggtggacga gatcaacggg atgctcgtcg acctcctccg ctagaaggga
     5161 gtccccatgg ccgatttttc ggatgaagat accagcgaag aggccctgaa gggcctcgag
     5221 gacgagggcc tggccggagg ccaagccgag gcggaagccg cggccgaagg cgaaggcgac
     5281 gcggaaagcc aggccgaggg cgagggccgg gagggcggcg gggcgctgtc cgggcagcgg
     5341 gagcgctccc ggggcgagca gcgcggccgc ggccgcgagc tgccctctcc cctctaccgc
     5401 atccgcgcga cccactccaa cgagaccttc ttcgcctggt gggagggcga aggcgagctt
     5461 gcgccccgga cctgggtcgt cgcgccgacc cgctacggcc gcgacgtctg cgagctccag
     5521 ggcctggtgc gctgccccgg ccacgtgggc gccgacgaga tcctcaagat cgaccggatc
     5581 gccaccgagg aggacctctc caaggtcaag gcgaacgagg agcgcgagcg caaggccttc
     5641 gagctctgca ggcagaagat agagacgagg cggctcccga tgaagctcgt cgccgcccac
     5701 tacctcttcg acgagcccaa ggtcctcttc ttcttctccg cggagtcgcg ggtcgacttc
     5761 cgcgagctcg tgaaggacct ggtctccgtc ttcaagatgc ggatcgagct caggcagatc
     5821 ggcgtccgcg acgaggcccg cgtgacgggc ggctgcggcg tctgcggccg cgtcctctgc
     5881 tgccacggcg tcacggacaa gctcaccccc gtctccatca agatggcgaa ggaccagaac
     5941 ctctccctca acagcatgaa gatctccggc ccctgcggcc gcctcctctg ctgcctggcc
     6001 tacgagtacg gcttctaccg cgacgcgcgc cgcgagctcc ccggcgaggg cgtgcgcttc
     6061 ctcttcgagg gcgtcaactt ccgcgtcgtc gaggtgaacg ccctggccgg ctccgtgaag
     6121 gccgccggcg aggacggccg cgtcctgtgg atgcaggccg cccgcttcat ccacagcgag
     6181 ggcaagtgga aggtccgcga ggagatcccg gaccccgacc gggcctagg
//
LOCUS       DAOOSJ010000167         6170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7900_length_6170_cov_5.309076, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000167 DAOOSJ010000000
VERSION     DAOOSJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7900_length_6170_cov_5.309076"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLG14_08765"
     CDS             <1..763
                     /locus_tag="PLG14_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49261.1"
                     /translation="VALEGSMLAGAFAAAVVAKATGSATLAFAAALLAGLLLALLVGT
                     SALRLGSDVFVAGLAANLLAPGAASLVSEALFGTKGVVAAPALGGSGHVLGLDPSFFV
                     LLGASALVVVLYSATPLGLRLRAAGEPGEAARAAGLRPERLRLAAHLVAGGASGLAGA
                     ALAAQVSAFVPGMSSGRGWIALVAIYLGGRRPGGVAAASLAFGALIALSNAAQAFRGA
                     PAELLLGLPYAATALALVLARAAARRRAARAGGPA"
     gene            764..1348
                     /locus_tag="PLG14_08770"
     CDS             764..1348
                     /locus_tag="PLG14_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49262.1"
                     /translation="MPSFVGKLASGLQERLASTWLLLSDTTNFLSRTSIFGQYEADLM
                     ELRRRLSSSRKSAELEKEVRKELVAIRESVRLQGYDLSLAKLELRVEGFRNDAALEEG
                     FLRLVLFIGKRGVWKLAGEANHIELHRSLDRGLAGREDDIVGKHYLWYRWIRQRLVLS
                     GSDTETREDFEAFKAWCAVPEHSLLLLAAMKNQR"
     gene            1517..1984
                     /locus_tag="PLG14_08775"
     CDS             1517..1984
                     /locus_tag="PLG14_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOX49263.1"
                     /translation="MFSVPTRTQYGIRALVHLAKAELASRERAGSLAREEAAQAHGSL
                     AAVATSAEIARAENISPKYLEGILAQLKAKGLVAAERGKNGGYRLSRDPGAILMSEVV
                     EALEGEVRPVDCVEDSGCCSQGGSCLPRRFWLGLKGAIDGYLSSKTLKDVLDA"
     gene            2011..3249
                     /locus_tag="PLG14_08780"
     CDS             2011..3249
                     /locus_tag="PLG14_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOX49264.1"
                     /translation="MASRFVYMDYNATTPLHPEVKEEMVADLEIYANASSMHEAGRRA
                     RSRVEKARASVARLVGARDPECLYFTSGGSESNNTVFNTMYDLGRRGTAGGSKPLPER
                     RQKIITTAIEHPCVLNAARRLEDEGFPVVFLGVDGQGRIDLSELERELDPSRSRGGPV
                     LLVSVMAANNEIGTVQDMAAISRLAKAAGAFFHTDATQAVGKIPVDVEAWGADYLTLS
                     CHKIYGPKGIGGLYARKGAPLEPLVRGGHQEKGVRAGTYNNLGIYGFGKAAELAMSEL
                     PAYAARVGALRERLRSGIVGRVPSVRVNGHPTEVLPNTLNVSFPGAEGEAILLSLDLE
                     GIEVSTGSACASGSLEPSHVLMATGVGPELAHGSIRFSLGRGTTEEEVDYVLEKLPPI
                     IARLRRMSTVPASCQDGTAS"
     gene            3304..3906
                     /locus_tag="PLG14_08785"
     CDS             3304..3906
                     /locus_tag="PLG14_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOX49265.1"
                     /translation="MSDAFEWLYSDVVKEHFTNPKNVFDPDEKFAADAEGIVGNIKCG
                     DQMLFLLKIQDDVITEVRWKTYGCASAIASTSILSEAVKGMGIREAYGIKPAEIAKRL
                     GGLPENKIHCSVLGDKALRAAIDSYLEKRGRAGEFKGEDAVVICTCLNITDKDIEEAV
                     KAGARDWERLQEATKIGTVCGGCKQRAEELLHEFSHIYGD"
     gene            4026..>6170
                     /locus_tag="PLG14_08790"
     CDS             4026..>6170
                     /locus_tag="PLG14_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49266.1"
                     /translation="MNIPIRRAALLALAAAALSALPLGAQAGDSGRGLGAPAQGRFQP
                     SSWRWSLGDGPERADPSFDDSSWTEAAPGDKISLGEPGAVFWLRTRIPQDGLESILAG
                     IAASGDEEGKGLWVISGKSGSAAEVYANGVYIGARGRLEPYDLRRTRSAAVHLPASLL
                     SPGEGLVLALRCSYRGSSLVLPAYTIGGATAVAFDLGPLNFWNGGLYSMLGALCVFLG
                     FYFLLQFAYRRAETENLYFGLSLVSIALYFYEMGAEYLVAGPALRALARGALPLSMAF
                     LFCFFSTFFRYRDRRVLRAGALAAGLASMALFLALMRDESNLDLAFTLSLLPVFSVIV
                     FGLFASLAAVRRGQREALPVLIGIAIGILFASYDIYHQATGIEPFAWLQGLAFFALNV
                     SIFFSLSMRQSRLKDELERLARETADGSARLEASVRRLERAGEAAAEIGRELDSSVSS
                     VALAVGRSDEGVSRVDRETLRQAGLAEEADGIVSGFLESIGRVNGSLSEQAEGVARTA
                     AAAVELSAGAESVAANIDRTAAFAGSLASLTAAGEKAVADLAAAVGRVLESSRGIAEI
                     VDAVNEFAERTNLLAMNAAIEAAHSGAAGRGFAIIAGEVKKLAQLQAERAARIAEIVA
                     EIGKRAEEGRVDTERVRSSLAEIAEGAKTAAENLAEVKAGTQEQTRASVEVRVGGENI
                     YGTTAALISGAGVTVQVLDSSEPVGQKGKDSKK"
BASE COUNT          869 a         2355 c         2031 g          915 t
ORIGIN      
        1 cgtggcgctg gagggctcga tgctcgcggg ggccttcgct gccgccgtcg tcgccaaggc
       61 gacgggctcc gcgacgctcg ccttcgccgc ggccctccta gccggcctcc tcctcgccct
      121 ccttgtcggg acctcggccc tgaggctcgg ctcggacgtc ttcgtcgccg gactagccgc
      181 caacctcctg gcccccgggg cggcctccct tgtctcggag gcgctcttcg gcacgaaggg
      241 ggtcgtcgcc gccccggccc tgggaggctc cggccacgtc ctgggcctcg atccctcctt
      301 cttcgtcctc ctgggcgcct cggccctcgt cgtcgtcctc tattcggcga cgccgctggg
      361 cctcaggctc cgcgccgcgg gcgagccggg cgaggccgcc agggcggcag gcctacgtcc
      421 cgagcggctc cgcctcgcgg cccacctcgt cgcgggcggc gcctcgggcc tggcaggggc
      481 cgccctggcg gcccaggtct ccgccttcgt ccccggcatg tcctcgggac ggggctggat
      541 agccctggtc gcgatctacc tcggggggcg caggcccggc ggcgtcgccg ccgcgagcct
      601 cgccttcggc gccctcatcg ccctctccaa cgcggcccag gccttcagag gcgcgcccgc
      661 cgagctcctc ctcggcctgc cctacgccgc gacggccctg gccctcgtcc tggcccgcgc
      721 cgccgcgcgg cgccgggcgg cgagggcggg agggccggcc taggtgccct ccttcgtcgg
      781 caagctcgcc tcgggcctcc aggagcgcct cgcctccacc tggctcctcc tctcggacac
      841 gaccaacttc ctctcgcgca cctcgatctt cgggcagtac gaggccgacc tcatggagct
      901 taggcgcagg ctctcctcct cgcgcaagag cgctgagctc gaaaaggagg tgaggaagga
      961 gctcgtcgcg atccgggagt cggtcaggct ccagggctac gacctgagcc tcgccaagct
     1021 cgagctccgg gtggagggct tccgcaacga cgcggccctg gaggagggct tcctccgcct
     1081 tgtcctcttc atcgggaagc ggggcgtctg gaagctcgcg ggggaggcca accacatcga
     1141 gctgcaccgc tccctggatc gcggcctcgc cggccgcgag gacgacatcg tcggcaagca
     1201 ctacctctgg taccgctgga tccgccagcg cctcgtcctc tcgggctccg acaccgagac
     1261 gagggaggac ttcgaggcct tcaaggcctg gtgcgccgtc cctgagcatt ccctcctcct
     1321 cctcgccgcg atgaagaacc agcgctgact cccgcgacgc gcggtcgcgc cggccgggcc
     1381 gccgcgccgt ccccgtctcg ttccccgcag ccggcccggc ggggccccgg cccggctttt
     1441 cgtccctttt cgcccttgac gaaaccgcgc cttcccggta tattcccact aacccgatag
     1501 gaaatacgga gtatacgtgt tctcagtgcc gaccaggacc caatacggga ttcgcgccct
     1561 cgtccacctc gccaaggcgg agctcgcctc gcgggagcgc gccggctccc tagcccgcga
     1621 ggaggccgcc caggcccacg gctccctggc cgccgtggcg acgagcgcgg agatcgcgcg
     1681 ggcagagaac atctccccga agtacctgga gggcatactc gcccagctca aggccaaggg
     1741 cctcgtcgcc gccgagcggg gcaagaacgg cggctaccgc ctctcccgcg accccggcgc
     1801 catcctcatg agcgaggtgg tggaggcgct ggagggcgag gtcaggcccg tcgactgcgt
     1861 ggaggactcg ggctgctgtt cccagggcgg ctcctgcctc ccccggcgct tctggctcgg
     1921 cctcaaggga gccatcgacg gctacctctc ctccaagacc ctgaaagacg tcctcgacgc
     1981 atgacaagct acggcaaagg agcgatcccc atggcttcca ggttcgtgta catggactac
     2041 aacgcgacga cccccctcca ccccgaggtg aaggaggaga tggtcgccga cctcgagata
     2101 tacgccaacg cctcgagcat gcacgaggcg ggccgccgcg cccgctcccg cgtggagaag
     2161 gcccgagcct cggtggcgcg cctggtcggc gcccgcgacc ccgaatgcct ctacttcacc
     2221 tccggcggct cggagtcgaa caacaccgtc ttcaacacga tgtacgacct gggacgccgc
     2281 ggcacggccg gcggctccaa gcccctgccc gagcgccggc agaagataat caccacggcg
     2341 atcgagcatc cctgcgtcct caacgccgcc aggcgcctcg aggacgaggg cttccccgtc
     2401 gtcttcctgg gcgtggacgg gcaggggagg atcgacctct ccgagctcga gcgcgagctc
     2461 gacccctccc gctcgcgcgg cggccccgtc ctcctcgtct ccgtgatggc ggcgaacaac
     2521 gagatcggca ccgtccaaga catggcggcg atctcccgcc tcgccaaggc ggccggagcc
     2581 ttcttccaca ccgacgcgac ccaggccgtc ggcaagattc ccgtggacgt ggaggcctgg
     2641 ggcgccgact acctcaccct ctcctgccac aagatctacg gccccaaggg cataggcggc
     2701 ctctacgcca ggaagggcgc ccccctcgag cctctcgtgc ggggcggcca ccaggagaag
     2761 ggcgtgcggg cgggcaccta caacaacctc ggcatctacg gcttcggcaa ggccgccgag
     2821 ctcgccatgt cggagctgcc cgcctacgcg gcgcgcgtgg gcgccctgcg ggagcggctc
     2881 cgctcaggca tcgtcgggcg cgtgccctcg gtccgcgtga acggccatcc gacggaggtc
     2941 ctcccgaaca ccctcaacgt ctccttccct ggcgccgagg gcgaggccat cctcctctcc
     3001 ctggacctcg agggcatcga ggtctccacg ggctcggcct gcgcctcggg cagcctcgag
     3061 ccctcccacg tcctcatggc gacgggcgtc ggtcccgagc tggcccacgg ctcaatccgc
     3121 ttcagcctgg ggcggggcac gacggaggag gaggtcgact acgtcctcga gaagctcccc
     3181 cccatcatcg cgcgcctgag gcgcatgtcc accgtccccg cctcctgcca ggacgggacc
     3241 gccagctaga caccatcgcc cggcgcggcc gacgcggccg caggcgccaa caaaggagat
     3301 accatgagcg acgccttcga gtggctctat tccgacgtgg tcaaggaaca cttcaccaac
     3361 cccaagaacg tcttcgatcc ggacgagaaa ttcgccgcgg acgcggaagg catcgtcggc
     3421 aacatcaagt gcggcgacca gatgctcttc ctcctcaaga tccaggacga cgtgatcacc
     3481 gaggtccgct ggaagaccta cggctgcgcg agcgccatag cctccacctc catcctctcc
     3541 gaggcggtca agggcatggg catccgcgag gcctacggca tcaagcccgc ggagatcgcc
     3601 aagcgcctgg gcggcctccc ggagaacaag atccactgct cggtcctggg cgacaaggcc
     3661 ctgagggctg cgatcgactc ctacctcgag aagcgcggca gggccggcga gttcaagggc
     3721 gaggacgccg tcgtcatctg cacctgcctc aacatcaccg acaaggacat cgaggaggcc
     3781 gtgaaggccg gcgcccgcga ctgggagcgc ctccaggagg cgacgaagat cggcaccgtc
     3841 tgcggcggct gcaagcagcg ggccgaggag ctcctgcacg agttttccca catctacggg
     3901 gactgagtag ggccggcggc gcggggggcc tttggctccg cgcggcgctc aggcggggac
     3961 ggggcgggcg cttggccgaa gtttggactt gcctccgccc cgtccttcgc gtatcctgtc
     4021 ccgcgatgaa catcccaatc cgccgcgcgg ccctgctggc cctcgccgcg gcagccctgt
     4081 ccgcgcttcc cctgggcgcg caggccggcg actccggtcg cggtcttggc gccccggccc
     4141 agggccgctt ccagccatcc tcctggcgct ggtccctcgg cgacgggccc gagcgcgccg
     4201 atccctcctt cgacgactcg tcctggaccg aggccgcccc cggcgacaag atctcgctcg
     4261 gcgagccggg cgccgtcttc tggctcagga ccaggatccc gcaggacggg ctcgaatcca
     4321 tcctcgcggg catagcggcc tcgggcgacg aggagggcaa gggcctctgg gtcatctccg
     4381 gcaagtcggg ctcggccgcg gaggtctacg cgaacggcgt ctacatcggc gcccggggca
     4441 ggctcgagcc ctacgacctc aggcgcaccc gctcggcggc agtccacctt ccggcgagcc
     4501 tcctttcgcc gggggagggc ctcgtcctcg ccctccgctg ctcctaccgc ggctcctccc
     4561 tcgtcctccc cgcctacacg ataggcggcg ccacggccgt ggccttcgac ctgggcccct
     4621 tgaacttctg gaacggcggc ctctactcga tgctcggcgc cctctgcgtc ttcctcggct
     4681 tctacttcct cctccagttc gcctaccgcc gcgcggagac ggagaacctc tacttcggcc
     4741 tgtccctcgt ctcgatagcg ctctacttct acgagatggg cgccgagtac ctggtcgcgg
     4801 ggccggccct gcgggccctc gcgcggggcg cgctccctct ctccatggcc tttctcttct
     4861 gcttcttctc gaccttcttc cgctaccggg accgtcgggt actcagggcc ggagccctgg
     4921 cggccggcct cgcctccatg gccctcttcc tggccctgat gcgcgacgag tccaacctgg
     4981 acttggcctt caccctctcc ctccttcccg tcttctccgt gatcgtcttc ggcctcttcg
     5041 cgagcctggc cgcggtgcgc cgcggccagc gcgaggccct gcccgtcctc atcggcatcg
     5101 cgataggcat cctcttcgcg agctacgaca tctaccacca ggcgaccggg atcgagccct
     5161 tcgcctggct ccaaggactg gccttcttcg ccctcaacgt ctccatcttc ttctccctct
     5221 cgatgcgcca gtcgcggctc aaggacgagc tcgagcgcct ggcccgcgag acggccgacg
     5281 gctcggccag gctcgaggcc tcggtgaggc ggctcgagcg ggcgggcgag gccgccgcgg
     5341 agatcggccg cgagctcgac tcctcggtct cctccgtcgc cctggccgtg ggccgctccg
     5401 acgagggcgt ctcacgggtc gaccgcgaga ccctgcgcca ggccggcctg gccgaggagg
     5461 cggacggcat cgtctccggc ttcctcgagt ccatcggccg cgtgaacggc agcctctcgg
     5521 agcaggccga gggggtcgcg aggacggcag cggccgcggt cgagctctcc gcgggggcgg
     5581 agagcgtcgc ggccaacatc gacaggacgg ccgccttcgc cgggagcctc gcgagcctca
     5641 ccgccgcggg agagaaggcg gtggccgacc tggcggccgc cgtcggccgc gtcctcgagt
     5701 cctcgcgcgg catcgcggag atcgtggacg cggtcaacga gttcgcggag cgcaccaacc
     5761 tcctcgcgat gaacgcggcc atcgaggccg cccattcggg cgcggcggga cgcggcttcg
     5821 cgatcatcgc gggcgaggtg aagaagctcg cccagctcca ggccgagcgc gccgcccgca
     5881 tagcggaaat cgtcgcggag atcggaaaac gggcggagga gggccgggtc gacaccgagc
     5941 gcgtccgctc ctcgctcgcg gagatcgccg agggcgcgaa gacggccgcg gaaaacctcg
     6001 ccgaggtcaa ggccggcacc caagagcaga cgcgcgcgag cgtcgaggtc agggttggcg
     6061 gcgaaaacat ttacgggaca acggcagcgc tcatctcagg cgccggtgtc accgtccagg
     6121 tgctcgactc cagcgaaccg gtggggcaga agggcaagga cagcaagaag
//
LOCUS       DAOOSJ010000168         6136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_7956_length_6136_cov_5.782437, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000168 DAOOSJ010000000
VERSION     DAOOSJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_6136_cov_5.782437"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..592
                     /locus_tag="PLG14_08795"
     CDS             203..592
                     /locus_tag="PLG14_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOX49267.1"
                     /translation="MSKRCISSAGAPAAIGPYSHAVETEGLLFVSGQLGIDPGTQALV
                     PGGVRAETERALLNLSSILAATGLGPADVLKTTVFLRDIGDFAAMNEVYASFFPSEPP
                     ARSAFQVAALPKGGSVEIEAIARRGER"
     gene            613..2022
                     /locus_tag="PLG14_08800"
     CDS             613..2022
                     /locus_tag="PLG14_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOX49268.1"
                     /translation="MESATSGTEFKREINTFQLTLICVGAIIGAGVFSLTGVGISYAG
                     PAAFLVYIVAALIGIVSTIPTMIAASACPTTGGYYKYISRFLDPTLAFYYMWNRIIGI
                     FSVSVVAMSFGQYVQVIFPKVPVEVSALGAILLFTVLNLFDVKLITKANKYFVYVLIA
                     ALVWFLVEGLPKADVTRLQGFFEKGFGNFVTALIMYMFALDGASVVINMGGEVKDPRK
                     SIPMAIIWGTLIAGVLYALLAFAATTAVDFRQLIPPVSKRAQPLGAFAKTFMSPAGFV
                     FFISGGAFLAILTTLNAGIMIQSRIFWAAARDGIFPKWFATLNKHRQPARVSVVVAVV
                     ISLPILLKFSLGFTTLITLVPGFLFSMMMPISVLFIEKKFPNLYRKSLMPLPKLWLYL
                     IVAFSLGVSLLLSWNGLLKIGLKNLYYFAGFFGLGTVYYFFMVARMRKQGRNYRDIKK
                     DYDKYWIEEEARLAALAAK"
     gene            2107..3282
                     /locus_tag="PLG14_08805"
     CDS             2107..3282
                     /locus_tag="PLG14_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOX49269.1"
                     /translation="MAYDFDTVVDRAPTSSLKWSYRKKFCGDEEVLPLWVADMDFPSP
                     PEVVEAIRRRAEHPIYGYPVRTDGYYDSLIAWMRRRNGWEIQRDWICFSPGVVPALNI
                     AVLAYTQPGDKVVVQTPVYYPFSSAVLSNGRQLVENSLKLEGGRYVMDFEDLERKLDS
                     RTKLLILCSPHNPVGRVWERGELERLVELCARKGVVIVSDEIHSDLILGGRKHVCTAA
                     LSPEAARMTVTLGAPNKTFNIAGLGIANAIIPDRRLREAFMNQAANLGLGIANVFGIV
                     AQEAAYSKGEAWLEELLSYLRGNYGRLVSFLAERLPSLSVLPLEGTYLAWIDCRRLGM
                     SDAELKAFFLKEAKLWLDEGTMFGSGGSGFMRINLACPRSLLDEALSRLEKALAKRG"
     gene            complement(3289..3585)
                     /locus_tag="PLG14_08810"
     CDS             complement(3289..3585)
                     /locus_tag="PLG14_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOX49270.1"
                     /translation="MPSTIAPCGFDCANCPVYRATLADAPAALASLWELWDPATRPAT
                     PEELRCRGCLSGTAVHIPFCAACAIRRAAMEKADGDAPEEADGPAPDKPDGAGR"
     gene            complement(3651..3989)
                     /locus_tag="PLG14_08815"
     CDS             complement(3651..3989)
                     /locus_tag="PLG14_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOX49271.1"
                     /translation="MDQLLAVCGLDCAACPAFVATKADDLAALAKLAEDWGRQFGFAA
                     TPENIRCHGCGAKDGVQIGHCAECAMRLCALAKGFATCAQCADFGCAKTEGFFKEVPE
                     AKARLEALRK"
     gene            complement(4042..5073)
                     /locus_tag="PLG14_08820"
     CDS             complement(4042..5073)
                     /locus_tag="PLG14_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOX49272.1"
                     /translation="MASSIEGESPLRKAFSRPDSRKARYDRGMRSERLFSLVLLLADG
                     KVHSAAALARRFGVGVRSVYRDMDLLSSLGVPLEALPGRDGGYRVLPGYALDRSVLDE
                     GELAAVASALRGIGRATGDAESEAARSKLAALLGKAPGRRRSWIRVEFAGGGRDRERI
                     EALRAAIEERRLLRIAYRDAEGRASEREVEPVAVAYLWQSWYLWAYCRLRGGWRLFKL
                     GRIESLRPLMARFEERPEPSEDAWRSEWGTSTQTEVLLEIAPEAGARAEEWFGKTERD
                     AEGRILVRADLPENEWLLGFLLSFGEGLKVLKPESLARALSERARRIWLSNAPEGESP
                     KFWKNADMN"
     gene            5072..5572
                     /locus_tag="PLG14_08825"
     CDS             5072..5572
                     /locus_tag="PLG14_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOX49273.1"
                     /translation="MDRSLALLLDLADAEFDGESFNGPSFMKTLEGLSPERAASVETH
                     EGYSAWEVAHHVAYYKYYIARALGASEAIEPYPLPKGKSGFAPPREATEAAYKELLGY
                     LRKAHRCATEAIRGADPAKLVETMPKWDIPFAKAAAWLCTHDTYHAAQIRNMGVPGLK
                     EPKEGD"
     gene            5735..>6136
                     /locus_tag="PLG14_08830"
     CDS             5735..>6136
                     /locus_tag="PLG14_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX49274.1"
                     /translation="MGRTKKIVDKSVVLEASLDLIEREGIEAFSTRRLAARLRISAMT
                     LYNYYENREAILREVALRGLSDFLASLAAELGRHPEVGRAPLLAYKLLAAEFMRLGKE
                     RPRLYLFLFDSGLEELRRDPEVAKYYGHLLEG"
BASE COUNT          961 a         2189 c         1967 g         1019 t
ORIGIN      
        1 cccacctctg tccgggccca ggcccaggcc caggcccagg cccaggccca ggcccgggct
       61 tcggcgtcgg gcgctctagg cttgactggt gcagctcggt taaaaaagtt gactttgcta
      121 aaaaaacgtt gacaaagatt tacatgtgtc aaataataac ctcgcactgt ccagagaaac
      181 ggaaaccgcc aagcgaggtt ctatgtccaa gcgatgcatc tcatccgcgg gcgctcccgc
      241 ggccataggt ccctactccc acgccgtgga gacggagggc ctcctcttcg tctccggcca
      301 gctcgggatc gatcccggca cccaggccct cgtcccgggc ggcgtccgcg ccgagaccga
      361 gcgggccttg ctcaacctgt cctcgatact cgccgcaacc ggcctcgggc ccgccgatgt
      421 cctcaagacc acggtcttcc tccgcgacat cggagatttc gcggcgatga acgaggtcta
      481 cgcgtccttc tttccctcgg agccgcccgc ccgctccgcc ttccaggtcg ccgcgctccc
      541 caaggggggg agcgtcgaga tagaagccat cgcccgtcgc ggcgagcgct agcacacaac
      601 gaggagagtc ctgtggaatc agcaacgtcc ggcactgaat tcaagcggga aatcaacacc
      661 ttccagctca cgctgatctg cgtcggcgcc atcatcggcg cgggcgtctt cagcctgacc
      721 ggcgtcggca tctcctacgc cggccccgcg gccttcctgg tctacatcgt cgcggccctc
      781 atcggcatcg tctccacgat cccgacgatg atcgccgcct cggcctgccc gacgacggga
      841 ggctactaca agtacatctc gaggttcctc gacccgaccc tggccttcta ctacatgtgg
      901 aaccgcatca tcggcatctt ctcggtctcc gtcgtcgcga tgtccttcgg ccagtacgtg
      961 caggtcatct tcccgaaggt gcccgtcgag gtctccgccc tcggcgcgat cctcctcttc
     1021 accgtcctca acctcttcga cgtgaagctc atcacgaagg ccaacaagta cttcgtctac
     1081 gtcctcatcg ccgccctggt ctggttcctt gtcgaggggc ttcccaaggc ggacgtcacg
     1141 aggctccagg gcttcttcga gaagggcttc ggcaacttcg tcaccgccct catcatgtac
     1201 atgttcgccc tcgacggcgc ctcggtcgtc atcaacatgg gcggcgaggt gaaggatccc
     1261 cgcaagtcca tccccatggc gatcatctgg ggcaccctca tcgccggcgt cctctacgcc
     1321 ctactcgcct tcgcggcgac cacggccgtc gatttccggc agctcatccc ccccgtctcc
     1381 aagcgcgccc agcccctggg cgccttcgcc aagaccttca tgtccccggc cggcttcgtc
     1441 ttcttcatct cgggaggcgc cttcctcgcc atcctcacga ccctcaacgc cggcatcatg
     1501 atccagtcgc gcatcttctg ggccgcggcg cgcgacggca tcttcccgaa gtggttcgcg
     1561 acgctcaaca agcaccgcca gccggcccgc gtgtccgtcg tggtcgcggt cgtcatctcc
     1621 ctccccatcc tcctcaagtt cagcctcggc ttcacgaccc tcatcaccct cgtcccgggt
     1681 ttcctcttct ccatgatgat gcccatctcc gtcctcttca tcgagaagaa gttcccgaat
     1741 ctctacagga aatccctcat gcccctgccc aagctctggc tttacctcat cgtcgccttc
     1801 tccctgggcg tgagcctcct cctctcctgg aacggcctgc tgaagatcgg cctcaagaac
     1861 ctctactatt tcgcgggctt cttcgggctc gggacggtat actacttctt catggtcgcc
     1921 cggatgcgga agcagggccg gaactaccgc gacatcaaga aggactacga caagtactgg
     1981 atcgaggaag aggcccgcct cgccgccctg gcggcgaagt aggggccttg ccgcggccgc
     2041 ccgcgggcgg cccatgcccg gcggcggccc ctagcgggcc gccgccttac ccataaggag
     2101 ctcccgatgg cctatgattt cgacaccgtc gtcgaccgcg cgccgacgag ctcgctcaag
     2161 tggagctaca ggaagaagtt ctgcggcgac gaggaggtcc tccccctctg ggtggccgac
     2221 atggacttcc cctcgccccc cgaggtcgtg gaggcgatca ggcgccgggc cgagcatccg
     2281 atctacggct acccggtgag gaccgacggc tactacgact cgcttatcgc ctggatgcgg
     2341 cggcgcaacg gctgggagat ccagcgggac tggatctgtt tctcgccggg cgtggtgccg
     2401 gctctgaaca tcgccgtcct cgcctacacc cagccggggg acaaggtggt cgtccagacg
     2461 cccgtctact atcccttctc ctccgccgtc ctctcgaacg gccgccagct cgtggagaac
     2521 agcctcaagc tggagggcgg gcgctacgtc atggacttcg aggacctgga gcggaagctc
     2581 gactcgagga ccaagctcct catcctctgc agcccccaca atcccgtggg ccgggtctgg
     2641 gagcggggcg agctcgagag gctcgtcgag ctctgcgcca ggaagggcgt cgtcatcgtc
     2701 tccgacgaga tccactcgga cctcatcctg ggcggaagga agcacgtctg cacggccgcg
     2761 ctctcgccgg aggccgcgcg gatgacggtc accctgggcg cgccgaacaa gaccttcaac
     2821 atcgccggcc tcgggatagc gaacgcgatc atcccggaca gacggctcag ggaagccttc
     2881 atgaaccagg cggccaacct gggcctgggg atagccaacg tcttcggcat cgtcgcccag
     2941 gaggcggcct actccaaggg ggaggcctgg ctcgaggagc tcctttccta cctgcgcggc
     3001 aactacggga ggctggtttc cttcctcgcc gagcgcctgc cctccctctc cgtcctcccc
     3061 ctcgagggga cctacctcgc ctggatcgac tgcaggaggc tcgggatgtc cgacgccgag
     3121 ctcaaggcct tcttcctcaa ggaggcgaag ctctggctgg acgaggggac gatgttcggc
     3181 tcggggggct cgggcttcat gcggatcaac ctggcctgcc cgcggagcct cctggacgag
     3241 gccctctcgc ggctcgagaa ggccctggcc aagcggggct gaggcgggct agcggccggc
     3301 gccgtcgggc ttatccgggg cggggccgtc cgcttcctcc ggggcgtcgc cgtccgcctt
     3361 ttccatcgcg gcgcgcctga tcgcgcaggc cgcgcagaag ggaatgtgga cggccgtgcc
     3421 ggagaggcag ccgcggcaac ggagctcctc gggcgtggcg ggccgggtcg ccggatccca
     3481 gagctcccag agggaggcga gggcggccgg cgcgtcggcc agggtcgcgc ggtagacggg
     3541 gcagttggcg cagtcgaagc cgcagggggc gatggtcgaa ggcatgggcg ggagtgtacg
     3601 cgtccgcccc gccttcggca aggaatagta gcttaacgcg gcccccgcgc ctatttccgc
     3661 agggcctcga gccgcgcctt ggcctccggc acctccttga agaagccctc cgtcttggcg
     3721 cagccgaagt cggcgcactg ggcgcaggtc gcgaagcctt tggcgagggc gcagaggcgc
     3781 atggcgcatt ccgcgcagtg gccgatctgg acgccgtcct tcgctccgca gccgtggcag
     3841 cggatgttct ccggcgtcgc cgcgaagccg aactgcctgc cccagtcctc cgcgagcttg
     3901 gccagggcgg ccaggtcgtc cgccttcgtg gcgacgaagg cggggcaggc ggcgcaatcg
     3961 agtccgcaga cggcgagaag ctggtccatg ggttcctcct tggggcgatg ggcgatgccc
     4021 catgatcgga gggcaaccct gtcagttcat gtcagcattt ttccagaatt tgggcgattc
     4081 gccttccggc gcgttggaaa gccaaatcct ccgcgcccgt tccgagaggg cccgggccag
     4141 gctctcgggc ttgaggacct taaggccctc gccgaaggag aggaggaagc cgaggagcca
     4201 ctcgttctcc ggcaggtcgg ccctgacgag gatgcgtccc tccgcgtccc gctccgtctt
     4261 gccgaaccac tcctcggccc gcgccccggc ctccggggct atctcgagga ggacctccgt
     4321 ctgcgtcgac gttccccatt cgctgcgcca ggcgtcctcg gagggctccg gcctctcctc
     4381 gaagcgggcc atgagggggc gcagggactc gatccgcccg agcttgaaga ggcgccagcc
     4441 gccgcggagg cggcaatagg cccaaaggta ccaggactgc cagaggtagg ccacggcgac
     4501 gggctcgacc tcgcgctcgc tcgccctccc ctcggcgtcg cggtaggcga tcctgaggag
     4561 gcgccgctcc tcgatggccg cgcgcagggc ctcgattcgc tcgcggtcgc ggccgccgcc
     4621 ggcgaattcc accctgatcc aggagcggcg ccgcccgggc gccttgccga ggagggcggc
     4681 gagcttggac cgggccgcct cgctctccgc gtcgccggtc gccctgccta tgccgcggag
     4741 ggcggaggcg acggccgcga gctcgccctc gtccaggacg ctccggtcga gcgcgtagcc
     4801 ggggagtacg cgatagccgc cgtcgcggcc cggcagggct tcgaggggca cgcccaggga
     4861 ggagaggagg tccatgtcgc ggtagaccga gcgtacgccg acgccgaagc ggcgggccag
     4921 ggcggccgcg ctgtggacct tgccgtcagc gaggaggagg acgagggaga agagccgctc
     4981 cgagcgcatg ccccgatcat agcgcgcctt gcgggaatcg gggcgcgaaa aggccttgcg
     5041 gagcgggctt tccccctcga tacttgaagc catggacaga agcctagccc ttcttctcga
     5101 cctggcggac gcggaattcg acggggagtc cttcaacggg ccgtccttca tgaagaccct
     5161 ggagggcctt tcgcccgagc gggcggcctc cgtggagacc cacgagggct actcggcctg
     5221 ggaagtcgcg catcacgtcg cctattacaa gtactacatc gcgcgggccc tgggggcctc
     5281 ggaggcgatc gagccctatc ccctgcccaa ggggaagtcg ggcttcgccc cgccccggga
     5341 ggcgacggag gccgcctaca aggagcttct cggctacctc aggaaggctc atcgttgcgc
     5401 gacggaggcg atccggggcg cagacccggc caagctcgtg gagacgatgc ccaaatggga
     5461 catccccttc gcgaaggccg cggcctggct ctgcacccac gacacctacc acgcggcgca
     5521 gatccgcaac atgggcgtcc cggggctcaa ggagcccaag gaaggggact agggccgcgc
     5581 cccgccgacc ttcccgtcct cggtccgacg cttcaccttg tcccgcgcgg tccgcgaagg
     5641 ccggtccgga actggcccgc ggctcggctt tttcccttcc gccccttgcg gcggagcggc
     5701 taggcgtaca atgatctaaa aaacggaggc cacggtgggc aggaccaaga agatcgtcga
     5761 caagagcgtc gtgctcgagg cttccctcga cctcatcgag cgcgagggca tcgaggcctt
     5821 ctccacgcgc cgcctggccg cgcggctcag gatctccgcc atgaccctct acaactacta
     5881 cgagaaccgc gaggctatcc tcagggaggt cgcgctccgc ggcctgtcgg acttcctcgc
     5941 ctccctggcc gccgagctcg ggcggcaccc cgaggtcggc cgcgcccccc tgctggccta
     6001 caagctcctg gccgccgagt tcatgcggct agggaaggag aggccgaggc tctacctctt
     6061 cctcttcgac tcggggctgg aggagctcag gcgggatccc gaggtcgcga agtactacgg
     6121 ccacctcctt gagggg
//
LOCUS       DAOOSJ010000169         6079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8039_length_6079_cov_6.454515, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000169 DAOOSJ010000000
VERSION     DAOOSJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8039_length_6079_cov_6.454515"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1211)
                     /locus_tag="PLG14_08835"
     CDS             complement(144..1211)
                     /locus_tag="PLG14_08835"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX49275.1"
                     /translation="MEASTAALRGIVDRYASEELFSGSVLVRGPSGELLAEARGFAHR
                     GFSVPNGVETRFDTASLGKLFTAACAFRLVDRGLLRLDEGVAEAAGLEGTRIPADVTV
                     RHCLTHSSGIADDADEEAGEDYEAIWKAKPCYMVREARDFLPQFAHKEPLFPAGSKAR
                     YNNCAYVLVGLAMERRTGLPFRDLVAREVLGPAGLSRTGYFTKDGVEPDVAEGYATVE
                     DDRGEAAGFRKNIYAYPPVGSPDAGIFTTTRDLARLFAAFEGEGFLSPASKAEFLEPK
                     IDAFTLKSGDKRRYGYCLEHDLSPDGIRFRYGKDGVNPGVAAIAMRYPSSGGFVAILS
                     NQDADVWSMCRELAKAAGFVG"
     gene            complement(1397..1879)
                     /locus_tag="PLG14_08840"
     CDS             complement(1397..1879)
                     /locus_tag="PLG14_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HOX49276.1"
                     /translation="MDGEHPNDPLHGMTLEAILLALVDRYGWEGLAERIPVRCFESEP
                     SLKSSLTFLRRTPWARAKVEALYLGKLRPRRPARKRPETGAAGRPSAAGRAPAAGPAT
                     GSATAPDSGKRRTPAAGPAAAPARGSAPAPEAGPADRPAPADKPAPRGRNRYPGLFED
                     "
     gene            complement(1932..2633)
                     /locus_tag="PLG14_08845"
     CDS             complement(1932..2633)
                     /locus_tag="PLG14_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOX49277.1"
                     /translation="MKTPRLARLILPAALALLALTLPSCTRRDGGSAEEPQAENGRAA
                     ARTAVVAFVEGEATVGGKKAEPGLELEGRFVASTGPGARLDLVFGERNVLSVGQNALV
                     EVDLAGVAVELRLDRGGVTSVLKKLERLAESDSFRIVTAQSIAGVRGTSFCVWADSAS
                     TYVCACNGAVRTIDAKGSNELELEAAHHQARLYVAKDGAVSVETAGMLHHTDESVESV
                     ASRIGYAIDWTKVDR"
     gene            complement(2686..3711)
                     /locus_tag="PLG14_08850"
     CDS             complement(2686..3711)
                     /locus_tag="PLG14_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49278.1"
                     /translation="MGGDLGEARPLGLLARILPFSAVDGPGNRAVFFLQGCDFDCAYC
                     HNPETRAPCSGCGACLPACPAGALSPAGGGSGAGALAFSRGAPGEAAAGRGDPRGGAQ
                     GAPPAWDRGRCRDCGACAAACPHGSSPRARLLSAEDALADIERFRPFLRGITVSGGEC
                     TLQPAFLASLLEAARARGLPGLVDTNGGRPLAAFPRVLRAAEGFMLDVKAWNPAEHLA
                     LAGADNGPVLENLRLLSGSGALFEVRTVLALRAGASAAGEGRGVGAASEDDPAAWAFD
                     LEETVRETAAVLRAARSRARYRLIRYRPFGVRPDRAAALREPGEALAARLAALAREAG
                     AAEVLVS"
     gene            complement(3816..5384)
                     /locus_tag="PLG14_08855"
     CDS             complement(3816..5384)
                     /locus_tag="PLG14_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HOX49279.1"
                     /translation="MDPVLDLIRDKTLTYRQKLSALARAGEDRAAPLSIPPAVQALRE
                     AGVVCDLHEGPAPYRPRYVLPDYAAYLERGSAFLRVEPPRDLLEAVFALLALYRHVPS
                     ITGYPVWLGDLDGLLEPFVLRDPAALGLGGELAPAAEQALRLFLEQIDRDNPDSFCHA
                     DLGPASTAVGRAVLRHQAKAGRAVPNLSLRYDPGLTPDSFAEAAAACALDAAKPSFAN
                     HRLFQAEFEALGLAPYAIASCYNGLPVGGGSCTLVRLNLAALAARALGPAAGPVDEAA
                     LAAFLSGPLAEAVQATLSYIDERVRFLVEESGFFESSFLAAEGLVSLGRFTAMFGLVG
                     LAECANALAGEGARGSAGRFGRGGPADALGLRVLAEIDRLVAAHRIPGLSAAGGRCLL
                     HAQVGIDTDRGVSPGVRVPIGEEPPLHEHLLCSAPFQAPFVAGSGDVFAFEPTARANP
                     GQIVDAVKGFFASGGRYFSCYAADSDLVRVTGYLVKRSEVERLRKGEAVANGATALGM
                     GAMDNLGVLDRARR"
     gene            complement(5624..>6079)
                     /locus_tag="PLG14_08860"
     CDS             complement(5624..>6079)
                     /locus_tag="PLG14_08860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX49280.1"
                     /translation="GEGRIEAIEPRGYLEALVAGIVQSRTEPAALACDIEAGTDAIPP
                     EAATPLALIVNEAVTNALKHGPGGGAARRVSLSLRQEGGSFLLAVEDDGPGRPADAKP
                     GLGTKLVAALAAQMGGTCSYGPALALATDDPTRPGTRLEVRFGRPKDGA"
BASE COUNT          792 a         2084 c         2466 g          737 t
ORIGIN      
        1 tctggatgag gcaacgaccc ccgcactagt tagatttttc gatgaggcaa cacgtcgcca
       61 tgccccgccg ccgaggaccg gccccggcgg agggaccgcc gcgggccggt ccccgcgacg
      121 cggcggcaca ggccgccgcc ccgctagccg acgaagccgg cggccttcgc gagctccctg
      181 cacatcgacc agacgtcggc gtcctggttc gagaggatcg cgacgaagcc gcccgaggag
      241 gggtagcgca tcgcgatggc cgcgacgccg ggattgacgc cgtccttgcc gtagcggaag
      301 cggatcccgt caggcgagag gtcgtgctcg aggcagtagc cgtagcggcg cttgtcgccg
      361 ctcttgagcg tgaaggcgtc gatcttcggc tcgaggaatt ccgccttcga ggcgggggag
      421 aggaagcctt cgccttcgaa ggccgcgaag agcctggcga ggtcgcgcgt cgtcgtgaag
      481 atgcctgcgt cgggggagcc cacgggcggg taggcgtaga tgttcttccg gaagccggcc
      541 gcctcgcccc ggtcgtcctc gaccgtcgcg tagccctccg ccacgtccgg ctcgaccccg
      601 tccttcgtga agtagccggt cctggagagg ccggccgggc cgaggacctc gcgggcgacc
      661 aggtcccgga aggggagccc cgtccgccgc tccatggcga ggccgacgag gacgtaggcg
      721 cagttgttgt agcgcgcctt cgagcccgcg gggaagagcg gctccttgtg cgcgaactgc
      781 gggaggaagt ccctggcctc gcggaccatg tagcagggct tggccttcca gatggcctcg
      841 tagtcctcgc ccgcctcctc gtcggcgtcg tcggcgatgc cggagctgtg cgtgaggcag
      901 tggcgcaccg tgacgtccgc gggaatcctc gtgccctcga ggccggccgc ctccgcgacg
      961 ccctcgtcca ggcggaggag gccccggtcg acgaggcgga aggcgcaggc cgcggtgaag
     1021 agcttcccga gggaggccgt gtcgaagcgc gtctccacgc cgttgggcac ggagaatccg
     1081 cgatgggcga aaccgcgggc ctccgcgagg agctcgcccg aggggccgcg gacgaggacg
     1141 gagccggaga agagctcctc cgaggcgtag cggtcgacga tgccgcggag ggcggcggtg
     1201 ctggcttcca tggggccatc ctttctgcgc ggcggcgtgg cgcgccgggg catgatagcc
     1261 cggccgcggc gccgtggaaa ggtaaaaaac ggctatgtcg gtccttggcc ggatcggccg
     1321 cggaccggga gtcaatacgg gaggcgtcgc gcgcggcgag gacggccgtg cgggcgggcg
     1381 ccttcccggg cggcgcctag tcctcgaaga gccccgggta gcggttgcgg ccgcggggcg
     1441 cgggcttgtc ggcgggggcg ggccggtcgg caggcccggc ctcgggggcg ggcgcggatc
     1501 cgcgggccgg agcggcggcc ggccccgcgg cgggggtgcg gcgctttccg ctgtccggcg
     1561 cggtcgcgga gccggtcgcc ggccccgcgg cgggcgccct tcccgcggcg gagggccttc
     1621 ccgcggcgcc cgtctccggc cttttcctgg caggccgccg gggccggagc ttcccgaggt
     1681 agagggcctc caccttggcc cgcgcccagg gggtgcggcg caggaaggtc aggctcgact
     1741 tgaggctcgg ctcgctctcg aagcaccgga cggggatgcg ctcggcgagt ccctcccagc
     1801 cgtacctgtc gacgagggcg aggaggatcg cctccagggt catcccgtgc aggggatcgt
     1861 tcgggtgttc gccgtccatg gagggagtct agcccggcgg gggcgaaaag cggaagcggg
     1921 cggccgggcc gctagcgatc gaccttcgtc cagtcgatcg cgtagccgat gcgcgaggcg
     1981 acggactcga cgctctcgtc cgtgtggtgg agcatgcccg ccgtctcgac cgagaccgcg
     2041 ccgtccttcg cgacgtagag ccgcgcctgg tggtgcgccg cctcgagctc gagctcgttg
     2101 gagcccttcg cgtcgatcgt gcggactgcc ccgttgcagg cgcagacgta ggtcgaggcc
     2161 gagtcggccc agacgcagaa ggaggtgccc ctcactccgg cgatcgactg ggcggtcacg
     2221 atcctgaagg agtcggactc cgcgagccgc tcgagcttct tgagaacgga ggtgacgccc
     2281 ccgcgatcga gcctgagctc gaccgcgacg cctgcgaggt ccacctcgac gagggcgttc
     2341 tggccgacgg agaggacgtt ccgttccccg aagacgaggt cgaggcgggc gcccggcccc
     2401 gtggaggcga cgaagcgccc ctcgagctcg agccccggct ccgccttctt cccgccgacc
     2461 gtcgcctcgc cttcgacgaa ggcgacgacg gccgtgcggg ccgcggcgcg gccgttttcc
     2521 gcctggggct cctcggcgga gcctccgtcg cgccgggtgc aggagggcag ggttagcgcg
     2581 agcagcgcca aggcagcggg caggatcagt cgtgcgagcc gtggagtctt catgcctcca
     2641 tgttcgccgc ttacggccgc gctgtcaaac ctcgggccgc gcgggctagg agacgaggac
     2701 ctcggctgcg ccggcctcgc gcgcgagggc ggcgaggcgg gcggcgagag cctcgccggg
     2761 ctctcggagc gccgccgccc tgtccggacg gacgccgaag ggccggtagc ggatgaggcg
     2821 gtagcgcgcg cgggagcggg cggcgcggag gacggccgca gtctcgcgga ccgtctcctc
     2881 taggtcgaag gcccacgcgg cgggatcgtc ctcggaggcg gcgccgaccc cgcggccttc
     2941 gccggccgcc gaggctcccg cgcggagggc gagcaccgtg cggacctcga agagggcgcc
     3001 cgagcctgag aggaggcgga ggttctccag gacgggaccg ttgtccgcgc cagcgagggc
     3061 gaggtgctca gccgggttcc aggccttcac gtcgagcatg aagccctcgg ccgcccgaag
     3121 gacgcgcggg aaggccgcga ggggcctccc gccgttcgtg tcgacgaggc cggggaggcc
     3181 cctcgccctc gccgcctcga ggagggaggc gaggaaggcc ggctggagcg tgcactcccc
     3241 cccggagacg gtgatcccgc ggaggaaggg ccggaagcgc tcgatgtccg ccaacgcgtc
     3301 ctcggcggaa aggaggcgcg cccggggcga gcttccatgc gggcaggctg ccgcgcaggc
     3361 gccgcagtcg cggcagcggc cgcggtccca ggcgggcggc gcgccctgcg cgcccccgcg
     3421 tggatccccg cgccccgccg cggcctcccc gggcgcgccg cgcgagaagg cgagggcgcc
     3481 cgctccggac ccgccgcccg ccggcgagag ggcgcccgcg gggcaggcgg gcaggcaggc
     3541 gccgcagccc gagcaggggg cgcgggtctc ggggttgtgg cagtaggcgc agtcgaagtc
     3601 gcagccctgg aggaagaaga ccgcccggtt ccccggcccg tcgaccgcgc tgaaggggag
     3661 gatcctggcg aggaggccga ggggccgggc ctcgccaagg tctccgccca agccgcccgt
     3721 cgggcctccg cgcggaccca cgacatgcga gccgccgggc gggccctcgc ccggatcccc
     3781 gtggggaaag ccgccgggcc cacggccggc tccgctcacc gccgggcccg gtccaggacc
     3841 cccaggttgt ccatcgcgcc catgccgagc gcggtggccc cgttcgcgac ggcctctccc
     3901 ttccggaggc gctccacctc ggagcgtttc acgaggtagc ccgtgacgcg gaccaggtca
     3961 gagtccgcgg cgtagcagga gaagtagcgg ccgcccgagg cgaagaagcc cttcaccgcg
     4021 tcgacgatct ggccggggtt ggctctcgcc gtgggctcga aggcgaagac gtcgcccgag
     4081 cccgcgacga agggcgcctg gaagggcgct gagcagagga ggtgctcgtg gaggggcggc
     4141 tcctcgccga tggggacgcg gacgcccggc gagacgccgc ggtccgtgtc gatgccgacc
     4201 tgggcgtgga ggaggcagcg tccgccagcg gcgctgaggc cgggtatccg gtgggcggcg
     4261 acgaggcggt cgatctccgc aaggacgcgg agaccgagcg cgtcggccgg gccgccgcgg
     4321 ccgaagcgcc ccgcgcttcc gcgcgctcct tcgcccgcga gcgcattcgc gcactcggcc
     4381 aggcccacga ggccgaacat cgcggtgaag cggccgaggg agacgaggcc ctcagccgcg
     4441 aggaaggagg attcgaagaa gcccgactcc tcgacgagga agcggacgcg ctcgtcgatg
     4501 taggaaaggg tagcctggac ggcctcggcg agggggcccg agaggaaggc cgcgagggcg
     4561 gcctcgtcga cggggccggc ggcgggcccg agggcgcggg cggccagggc cgcgaggttg
     4621 agccggacga gggtgcagct cccgccgccg acggggaggc cgttgtagca gctcgcgatc
     4681 gcgtagggcg cgaggcccag tgcctcgaac tccgcctgga agaggcggtg gttcgcgaag
     4741 gagggcttgg ccgcgtcgag ggcgcaggcg gccgccgcct ccgcgaagga gtcgggcgtg
     4801 agcccggggt cgtagcgcag gctcaggttc ggcaccgccc ggcccgcctt ggcctggtgg
     4861 cggaggacgg ccctgccgac ggcggtcgag gccggcccca ggtcggcgtg gcagaaggag
     4921 tcgggattgt cgcggtcgat ctgctccagg aagaggcgga gggcctgctc cgcggccggg
     4981 gcgagctcgc cgcctaagcc gagggcggcc gggtcgcgga ggacgaaggg ctcgaggagg
     5041 ccgtcgaggt cgccgagcca gacgggatag ccggtgatgg agggcacgtg gcggtagagc
     5101 gcgaggaggg cgaagaccgc ctccaggagg tcgcggggcg gctcgacgcg gaggaaggcc
     5161 gagccgcgct ccaggtaggc cgcgtagtcc gggaggacgt agcggggcct atacggcgcg
     5221 gggccctcgt ggaggtcgca gaccacgccg gcctcgcgga gggcctggac ggcgggcggg
     5281 atggacaggg gggcggcgcg gtcctcgccg gcgcgggcca gggcggagag cttctggcgg
     5341 taggtgaggg tcttgtcgcg gatgaggtcg agaacgggat ccatgcgtcg ctcctcgcgg
     5401 gacggctcgt cccgggcggt gattttaacc gcggcttgca agcgcccgct tcctgggcgg
     5461 gcggcttggt cgggcccgag gcgcgctctg gcgcgagggg cctggcctgc ggttatttat
     5521 cgcggcgacg cgcgatgttg ccctcattgt aacggcggcc gcccgggggc ggcaagcggc
     5581 gaccgcgcgg ggcgccgcag accgccccgc cgctcggccc cgcctaggcc ccgtccttcg
     5641 ggcggccgaa gcggacctcg aggcgggtgc cgggtcgcgt cgggtcgtcc gtcgccagcg
     5701 ccagggccgg tccgtaggag caggtcccgc ccatctgcgc cgcaagggcg gcgacaagct
     5761 tggtgccgag gcccggcttg gcgtcggcgg gccgccccgg gccgtcgtcc tcgacggcga
     5821 ggaggaagga gccgccctcc tggcggaggc tgagggaaac ccgccgcgcg gcccccccgc
     5881 ccgggccgtg cttgagggcg ttcgtcacgg cctcgttgac gatgagggcg aggggcgtcg
     5941 ccgcctcggg agggatcgcg tccgtccccg cctcgatgtc gcaggcgagg gcggcgggct
     6001 ccgtcctcga ctggacgatg ccggcgacca gggcctcgag gtagccgcgg ggctcgatgg
     6061 cctcgatccg cccctcgcc
//
LOCUS       DAOOSJ010000170         5989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8213_length_5989_cov_5.707786, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000170 DAOOSJ010000000
VERSION     DAOOSJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8213_length_5989_cov_5.707786"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1418)
                     /locus_tag="PLG14_08865"
     CDS             complement(294..1418)
                     /locus_tag="PLG14_08865"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOX49281.1"
                     /translation="MDDPERDGLEGVEDLEPIDEPGEAARDAELARERRSLLSSPCLR
                     ALARSYERSPAPYAVVDAELRLIYRNPRFRALIASYEYEERETLLATFARAMPQESAL
                     DLREAVADDARGRLWKGILAHKSRDAPAHFTRMHVQPLWLTSERSKAPEVFVVYLDDV
                     TEENKKHLRGMFSSLLEASKMKDNDTGRHIERVNLYARLLARELYGDPRWPEVDVDFI
                     EEIGFLAAMHDVGKIGTPDDILNKNGPLSEFEWSIMKEHTINGAFILSSYPTPMAKQI
                     ALSHHEWWNGQGYPYNLEGAMIPLSARIVSVADVYDALRMRRSYKPAYPHEVALEKML
                     EEKAVHFDPEIIDAFLKIEAGFQEIYEEHEDQGQPAEGRR"
     gene            complement(1454..2149)
                     /gene="tsaB"
                     /locus_tag="PLG14_08870"
     CDS             complement(1454..2149)
                     /gene="tsaB"
                     /locus_tag="PLG14_08870"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOX49282.1"
                     /translation="MNVLALDSSTDLLSVALVTPAGKAEASLDLGLKHAERIVDLVDF
                     CLSRASLKPSDLDLVACARGPGSFTGLRIGMATAKGMALALGKPWISVPTLDCLAWGR
                     EAFQGAVAPVLDGRRGRVYTAIYERGARVTDWLDVPLARLAELLDTYPEALLTGPDAD
                     LFEELAAERSGFHVDRRARDPAARALAALAAEAFARDGGSAPDEGPLYLRPSEAEESA
                     DAGKAKGRDGGPP"
     gene            complement(2146..2658)
                     /gene="tsaE"
                     /locus_tag="PLG14_08875"
     CDS             complement(2146..2658)
                     /gene="tsaE"
                     /locus_tag="PLG14_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOX49283.1"
                     /translation="MAPPPPPPLPEGAPGRARQERGPSLRELELSSPEETEALGELIG
                     RRASGGLVIAFRGDLGAGKTVMAKGIARGLGVEDEVTSPTFTVVSEYEGRLRLHHVDA
                     YRLLGPADFEELGGEDLLADPGGVCLVEWSERIEAALPPDAAFVELSVLPDGRRRARA
                     WGEALERILP"
     gene            complement(2583..2843)
                     /locus_tag="PLG14_08880"
     CDS             complement(2583..2843)
                     /locus_tag="PLG14_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49284.1"
                     /translation="MKVVGLKDMRKKDTPLHYIREWSGVAVLENAGKTAERPFVCSIE
                     RRPVGAPEITARFLEDPEWPLLPLLRSLKELLAELDKSGGLP"
     gene            complement(2897..3988)
                     /locus_tag="PLG14_08885"
     CDS             complement(2897..3988)
                     /locus_tag="PLG14_08885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX49285.1"
                     /translation="MNAQLVLAQLYWKKTDPREGTTALIVVGVAVGLLLVYQIVKQLG
                     SGKGGLAGGSGSFSRGAFRRAAREGGLAEEELRFLEEYGKVFRITNPEFLFKNKAKLD
                     GFFKEAYRYIEKNSESEPAAEERKARLFAIRERLAKREALGAPVTSTRQLGRGVPLSF
                     IAPGGENYPSVIVAVEPGGLAVEPARDPYGEAIRFRKGSKLTCYFYTKGHQGFQFQAR
                     VAGWERIGPREVMVLAHSEAVTALPARSHVRREIRVPCTYYRVAVTTTRQRGKEKSNA
                     KVEKIPFPGTIVDISAGGLGIQCASPIDAGDFLKIEFNPGGGSQAAFGKVVRMNRLKG
                     TGGVMHIQFVKISQRGLNAILSYVYGYGE"
     gene            complement(3985..4920)
                     /locus_tag="PLG14_08890"
     CDS             complement(3985..4920)
                     /locus_tag="PLG14_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49286.1"
                     /translation="MDILVNGEPLALELAPGASVGEALARADELLEGSGSIIIGLELD
                     GRALDAEGFPAVRDLPASSVARVDIAVESAAGVRSKALSTLLEILALAREAAAAEDSA
                     GADWKSIAEGAAELAEAFAGLFSADELSFVQDIAALLAKAAEAAGGASAPDGVAAGPD
                     AALRADIRLRAEGVQGLARERLSEIEDPAGEMRKAAMLYRSQAADLAELPVLLQTGKD
                     ERAMRIVLLFIETFNKVIRLIPELRRQGFDTDALRVEGSSLPDFYASFNEVLRSLSRA
                     FEDKDAVQIGDLAEYEVAPRMAGFFAAIEEALLRK"
     gene            complement(4943..>5989)
                     /gene="fliD"
                     /locus_tag="PLG14_08895"
     CDS             complement(4943..>5989)
                     /gene="fliD"
                     /locus_tag="PLG14_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOX49287.1"
                     /translation="LLGPGGSTKPLPALEDSSGTATLKVELGSLLPEASALAFRSRDT
                     TRRLEVVSAKVYDPAESGGYKPRRPVSTAQDALVSVDGIEVTRTKNDIDDLVPGVTLN
                     LKSAGEKPVRLDVAPDREAAKEAVIAFVGNYNRLMADINILSRPDERLISEISYFTEE
                     EKKTAKDRLGLFQGDSTLGLLRTSLQRTMMEPYETAAGAELALLSQVGVATDARRPGG
                     GAGFDAARMRGYLEIEEDLLDKALAERFDAVKQLFGNDTDGDLIADSGAAFRLDNLLK
                     PYVETGGILSLKTGTLDRQIASEKKSIESLDKQLVAKEDELKRKYGMMEGSLNRMESS
                     SSAIDNFTQQQSGQ"
BASE COUNT          801 a         2028 c         2218 g          942 t
ORIGIN      
        1 cgaaccacgc ggtgcagagc ctctggaagg acttcgagcg ccgccagcac aagcggcgcc
       61 gcgcctactt caagaagctg tagcgaaggg ggcgccgggc agtgacgccg gtacgtggcg
      121 ccgcgccccg tagacgaagg aggccgcctg gggaatcccc gggcggcctc ctcgcttttt
      181 gttccgtcgc gcgcggcgcg tcgggctccc ctggcgtcgg acgcgtcgcg ccccgccggc
      241 cgcggacggc ggccttcgcg gcacggacgc cgcggcggcg gcgcgcggct ttcctagcgg
      301 cgcccctccg cgggctggcc ctggtcctcg tgctcctcgt agatctcctg gaagcccgcc
      361 tcgatcttca ggaaggcgtc gatgatctcg gggtcgaagt gcaccgcctt ctcctccagc
      421 atcttctcca gcgcgacctc gtgggggtag gcgggcttgt agctgcgccg catgcgcagg
      481 gcgtcgtaga cgtccgccac gctgacgatc cgcgcggaga gggggatcat cgccccctcc
      541 aggttgtagg gatagccctg gccgttccac cactcgtggt gggagagggc gatctgcttg
      601 gccatcggcg tcgggtagct cgagaggatg aaggcgccgt tgatcgtgtg ctccttcatg
      661 atggaccact cgaactcgga gaggggcccg ttcttgttga ggatgtcgtc gggcgtgccg
      721 atcttcccga cgtcgtgcat cgcggccagg aagccgatct cctcgatgaa gtcgacgtcc
      781 acctcgggcc agcgcgggtc gccgtagagc tcccgcgcca ggaggcgggc gtagaggttc
      841 acccgctcga tgtggcggcc cgtgtcgttg tccttcatct tcgaggcctc gaggagggag
      901 gagaacatgc cgcgcaggtg cttcttgttc tcctcggtca cgtcgtccag gtagacgacg
      961 aagacctccg gcgccttcga gcgctccgag gtgagccaga gcggctggac gtgcatccgc
     1021 gtgaagtggg ccggcgcgtc cctggacttg tgggcgagta tccccttcca caggcggccc
     1081 cgcgcgtcgt cggcgaccgc ctcgcggagg tccagggccg actcctgcgg catggcgcgg
     1141 gcgaaggtcg cgaggagggt ctcccgctcc tcgtactcgt agctcgcgat gagggcgcgg
     1201 aagcggggat tgcggtagat gaggcggagc tcggcgtcga cgacggcgta gggcgcgggc
     1261 gagcgctcgt agctccgcgc cagcgcgcgg aggcaggggg aggagaggag gctgcgccgc
     1321 tcgcgcgcga gctcggcgtc ccgggcggcc tcgccgggct cgtcgatcgg ctcgaggtcc
     1381 tctacgccct ccagcccgtc gcgttcagga tcgtccatcg cgctccctgt ccccgccgag
     1441 tcccggccgt ccctcacgga gggccgccgt cccgcccctt cgccttgccc gcgtcggccg
     1501 actcctcggc ctcgctcggc cgcaggtaga gcgggccctc gtcgggcgcc gagcctccgt
     1561 ccctggcgaa ggcctcggcg gcgagcgccg cgagggccct cgccgcggga tcccgcgccc
     1621 ggcggtccac gtggaagccg ctccgctcgg ccgcgagctc ctcgaagagg tccgcgtcgg
     1681 ggccggtcaa gagcgcttcc gggtaagtat cgagcagctc ggccagccgc gcaaggggaa
     1741 cgtcgagcca atccgtgacg cgcgcccccc gctcgtagat cgccgtgtag acccggcccc
     1801 gccggccgtc caggacggga gccacggcgc cttggaaggc ctcgcggccc caggccaggc
     1861 agtcgagggt cggcacggag atccagggct tcccgagggc cagggccatg cccttcgcgg
     1921 tcgccatccc gatgcggagg cccgtgaagg agccgggccc gcgggcgcag gccacgaggt
     1981 ccaggtccga cggcttgagg gaggcgcggg agaggcagaa gtcgacgagg tcgacgatcc
     2041 gctcggcgtg cttgaggccc aggtccaggc tcgcctcggc cttgcccgcg ggcgtgacca
     2101 gggcgacgga gaggaggtcg gtggaggagt ccagggcgag cacgttcacg gcaggatcct
     2161 ttcgagggcc tcgccccaag cgcgggccct gcgcctcccg tcggggagga cggagagctc
     2221 gacgaaggcc gcgtccgggg ggagggcggc ctcgatgcgc tcgctccact cgacgaggca
     2281 gacgccgccc gggtccgcga ggagatcctc gccgccgagc tcctcgaagt cggctgggcc
     2341 gaggaggcgg taggcgtcga catggtggag gcggaggcgc ccctcgtact cggagaccac
     2401 ggtgaaggtg gggctggtaa cctcgtcctc gacgcccagg ccgcgcgcga tgcccttggc
     2461 catcacggtc ttgccggcgc ccaggtcgcc gcggaaggcg atgacgaggc cgcccgaggc
     2521 ccttcggccg atgagctcgc ccagggcctc ggtctcctcg gggctcgaca gctcgagctc
     2581 cctcagggaa ggcccccgct cttgtcgagc tcggccagga gctccttcag ggagcggagg
     2641 agggggagga ggggccactc ggggtcctcg aggaagcgcg cggtgatctc cggggcgccc
     2701 acggggcgcc gctctatgga gcacacgaag ggccgctcgg ccgtcttgcc ggcgttctcg
     2761 agcacggcca cgccgctcca ctcgcgtatg tagtggaggg gcgtgtcctt cttccgcatg
     2821 tccttgagcc cgacgacctt catgaggtca ttgtacggaa agcctaaggc ggcagtcaag
     2881 ttgagcgccc cgcccgctat tcgccgtagc cgtagacgta ggagaggatc gcgttcaggc
     2941 cgcgctgcga gatcttcacg aactggatgt gcatcacccc gccagtgccc ttgaggcggt
     3001 tcatgcgcac caccttgccg aaggccgcct gggagccgcc gccggggttg aactcgatct
     3061 tgaggaagtc ccccgcgtcg atcgggctcg cgcactggat cccgaggccg ccggccgaga
     3121 tgtcgactat cgtgccgggg aaggggatct tctcgacctt ggcgttcgac ttctccttgc
     3181 cgcgctgcct cgtcgtcgtg accgccacgc ggtagtaggt gcagggcacg cggatctccc
     3241 gccggacgtg gctccgggcg ggcagggcgg tcaccgcctc gctgtgggcc aggaccatga
     3301 cctctcgggg gccgatcctc tcccagccgg cgacgcgggc ctggaactgg aagccctggt
     3361 ggcccttggt gtagaagtag caggtgagct tcgagccctt cctgaagcgg atggcctcgc
     3421 cgtaggggtc ccgggccggc tcgacggcga gcccgcccgg ctcgacggcg acgatgacgg
     3481 aggggtagtt ctccccgccc ggcgcgatga aggagagggg gactccgcgg ccgagctgcc
     3541 tcgtcgaggt gaccggcgcg cccagggcct cgcgcttggc caggcgctcc cggatcgcga
     3601 agagccgggc cttccgctcc tcggccgccg gctccgactc cgagttcttc tcgatgtagc
     3661 ggtaggcctc cttgaagaag ccgtccagct tggccttgtt cttgaagagg aattccgggt
     3721 tcgtgatcct gaagaccttg ccgtactcct ccaggaagcg gagctcctcc tccgcgaggc
     3781 cgccctctcg cgccgcccgc ctgaaggcgc cgcggctgaa ggagccggac ccgcccgcga
     3841 gcccgccctt tccggagccg agctgcttga cgatctggta gacgaggagg aggcctacgg
     3901 cgacgccgac gacgatgagg gcggtcgtgc cctcgcgggg gtcggtcttc ttccagtaga
     3961 gctgggccag gacgagctgc gcgttcattt ccgtaggagc gcctcctcta tcgcggcgaa
     4021 gaagccggcc atcctggggg cgacctcgta ctcggcgagg tcgccgatct ggacggcgtc
     4081 cttgtcctcg aaggcccggg agaggctcct caggacctcg ttgaaggatg cgtagaaatc
     4141 cggcagggag gagccctcga cgcgcagggc gtcggtgtcg aagccctgcc tgcggagctc
     4201 ggggatgagc ctgatgacct tgttgaaggt ctcgatgaag aggaggacga tccgcatggc
     4261 ccgctcgtcc ttccccgtct ggaggaggac cgggagctcg gccaggtcgg cggcctggct
     4321 gcggtagagc atcgcggcct tgcgcatctc cccggcggga tcctcgatct ccgacagccg
     4381 ctcccgggcc aggccctgga cgccctcggc gcggagcctg atgtcggccc tgagggccgc
     4441 gtcggggccg gccgcgacgc cgtccggcgc cgaggccccg cccgcggcct ccgccgcctt
     4501 ggcgaggagg gccgcgatgt cctggacgaa ggagagctcg tccgcggaga agaggccggc
     4561 gaaggcctcc gcgagctcgg cggcgccctc cgcgatcgac ttccagtcgg ctccggccga
     4621 gtcctcggcc gcggcggcct cgcgggcgag ggccaggatc tcgaggaggg tggagagggc
     4681 cttggagcgc acgccggccg ccgactccac ggctatgtcg acgcgggcga cggaggaggc
     4741 gggcaggtcc cgcaccgcgg gaaagccctc cgcgtccagg gcccttccgt cgagctcgag
     4801 gccgatgatg atcgaccccg atccctcgag gagctcgtcg gcccgggcga gggcctcgcc
     4861 gacgctcgcg ccgggcgcga gctcgagggc gaggggctcg ccgttcacca ggatgtccat
     4921 aggaggggct ccttccaggc ggctactggc cgctctgctg ctgcgtgaag ttgtctatgg
     4981 ccgaggagct gctctccatg cggttgagcg atccctccat catgccgtac ttgcgcttga
     5041 gctcgtcctc cttggcgacg agctgcttgt ccaggctctc gatgctcttc ttctcggagg
     5101 cgatctgccg gtccagggtc ccggtcttga gggagaggat gccgccggtc tcgacgtagg
     5161 gcttgaggag gttgtcgagc ctgaaggccg cgcccgagtc ggcgatgagg tcgccgtccg
     5221 tgtcgttgcc gaagagctgc ttcaccgcgt cgaagcgctc ggccagggcc ttgtcgagga
     5281 ggtcctcctc gatctcgagg tagccgcgca tccgggccgc gtcgaagccc gccccgcctc
     5341 ccggcctcct cgcgtcggtg gcgacgccga cctgcgagag gagggcgagc tccgccccgg
     5401 ccgccgtctc gtagggctcc atcatcgtgc gctggaggct ggtgcggagg aggcccaggg
     5461 tcgagtcgcc ctggaagagg ccgagcctgt ccttcgcggt cttcttctcc tcctcggtga
     5521 agtaggagat ctccgagatg aggcgctcgt cgggccgcga gaggatgttg atgtcggcca
     5581 tgaggcggtt gtagttgccc acgaaggcga tcaccgcctc cttggccgcc tcccggtcgg
     5641 gggccacgtc caggcggacg ggcttctccc ccgccgactt gaggttcaag gtgacgccgg
     5701 gcacgaggtc gtcgatgtcg ttcttcgtcc tcgtcacctc gatgccgtcg acggacacga
     5761 gggcgtcctg ggccgtggag acggggcgcc tgggcttgta gccgcccgat tccgcggggt
     5821 cgtagacctt ggcgcttacg acctcgaggc gcctcgtcgt gtcccgggag cggaaggcga
     5881 gggcggaggc ctcggggagg agggagccga gctccacctt gagcgtggcg gtcccggagg
     5941 agtcctcgag ggcggggagg ggcttggtgg agccgccggg gccgaggag
//
LOCUS       DAOOSJ010000171         5979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8236_length_5979_cov_5.110736, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000171 DAOOSJ010000000
VERSION     DAOOSJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8236_length_5979_cov_5.110736"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1282
                     /locus_tag="PLG14_08900"
     CDS             98..1282
                     /locus_tag="PLG14_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOX49288.1"
                     /translation="MRSRGNRGAARALLAAAALAAAGLLSLSCSKPGPEAGQKGEEPL
                     RIAIAFDVGGRGDGAFNDSAYAGLVQVAKAYGGKIVGDPDRVDHGGKIELRYLESKLG
                     GQDREQLLRVLSEEGNRLVFGVGFMFAESIARVAKDFPNVHYALVDGVVPDLAEGSNV
                     TCLGFAEEEGSFLVGALSGLAVADKPGAKVGFIGGMDTPLIHRFQAGFAAGAAYANPR
                     LRKPGMVLGQYIGRDGSAFNDPKTAAAIASTMYKGGAEIVYHAAGGSGAGLFEAAAAA
                     GKLAVGVDSDQGYSLMTDAKDPAARELGGRVLTSMLKRVDQAVFLTAKELVESGKVRG
                     GYRTFSLADDGVGVAKNEFNSGKLAPYEAELDALKARIVAGEIKVPADAEAAAAFVKG
                     LK"
     gene            complement(1392..3536)
                     /locus_tag="PLG14_08905"
     CDS             complement(1392..3536)
                     /locus_tag="PLG14_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOX49289.1"
                     /translation="MSPRPKLRKFLFLIVFALALVLPALAEEGTGGVTASMTSLVIQL
                     GLIIIVAHLGGSLASKVGLPSVLGELAAGILLSQGALGGVALPGFPAGLFPAAASGFA
                     VSTELYGFATLASIVLLFMSGLETDISLFKRYAAAGLAVGLGGVAASFGLGALCGVLA
                     FHWTWLDPRSLFLGILGTATSVGITARILSDQRKMDSPEGTTILAAAVIDDVLGIICL
                     AVVLGISSVLSSGSGAVNWGRIGGIAAKAFGIWAVFTLLGLAFSRRIARFLKRFRTPE
                     AFAILALGLALLLAGFFETEGLAMIIGAYIMGLSLSGTDLKLVIREKLQPLYMFLVPI
                     FFAVMGMLVDLKELSSPEVLAFGAVYTFVAFGSKIVGCALPSLFFGFNPLGALRVGVG
                     MVPRGEVALIIAGIGISGGFLDEKLFGVVIMMTLLTTVVPPPFLKLLLAKPGAGTKTP
                     QAGSETGTIEIPIEDPGIVELVVESMAAQLRHEGFFVRLADPESDIYVAAREDSRIVI
                     RPEGAGIVLEASKKSLYLAKAAAHETILELTDSFRRAGKSLDAESLRIGLVGASSEET
                     AKTALIPPECVKLGLKARDKEEAIRKLLGVLKAAGKIGDEEEAAVDVLAREHIGATGL
                     ERGVAFPHGRSDAAKKPAAALGVFEQGIDFGAADGIPARVVLLLVSPKGEEVPHLGLM
                     AGLAASLSREGALETILRANYPAEAAKVFSAK"
     gene            complement(3810..3895)
                     /locus_tag="PLG14_08910"
     tRNA            complement(3810..3895)
                     /locus_tag="PLG14_08910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3859..3861),aa:Leu,seq:taa)
     gene            4012..5229
                     /locus_tag="PLG14_08915"
     CDS             4012..5229
                     /locus_tag="PLG14_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49290.1"
                     /translation="MDSDYLLAGGRFVLPEAVLDGGALLVEKGRIARIYAAGEPLPTG
                     PARIDAKGAWVTPGLMELHIHGAGGVGFEALGLGASKRAGAGAGPDEMDRPGEPERTG
                     YAEAAARDLEGVAVFLEARGVTAFLPTLVAEPRSAERLALALETAAFPAHRVPGIYIE
                     GPFVNPARRGGLPQEFLREVGPKILDSYLQAARGRLKVMTYAPELPGADSLPGRLAEE
                     GVLPALGHSDAVLGRFEPPEGPYSLTHHFNAMSPFSHKEPGLAMLPFLDPRPSVELVS
                     DGVHVNDEALRICAAAFGSGRILLISDACAAAGLPDGEYEYFGRRIVSGERGVRYADS
                     GVLMGSRRLGPELLRNWLRATGRPVHEAVLALTRNPRRLVGVLDRGSIEPGQAAELVV
                     WEGEFEAPRLVLR"
     gene            5324..>5979
                     /locus_tag="PLG14_08920"
     CDS             5324..>5979
                     /locus_tag="PLG14_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOX49291.1"
                     /translation="MRSTCLYESDGGSPRRILCFGDSNTWGYEPGTGRRFAEGVRWPG
                     VLASELGAGFRVVEEGLCSRTTVFEDPLAAGRRGIEALPTALESHAPLDLVVLMLGTN
                     DLKRRYASGAYEIALGLERLARLVLSSGAGRASPDGTAAEAPALLLVAPPPLGALGEN
                     GAAFEGGRAVSLELPARVAALAAALAAEGRAVGHLDAGAAAAAGSDGIHLDPAAHAAL
                     G"
BASE COUNT          885 a         2083 c         2186 g          825 t
ORIGIN      
        1 tggccctcct cgtcttcgcc ggcaaggcgg tcgggcccaa ggccgcgggc gagatctacg
       61 acgcgggcaa gcgatgacgg agcaaggagc ggcaagcatg agatcgagag gcaaccgggg
      121 cgccgcgcgc gcccttctcg cggcggcggc cctggcggcc gccggcctcc tttccctttc
      181 ctgctccaag cccgggcccg aggccgggca gaagggcgag gagcccttga ggatcgccat
      241 agccttcgac gtcggcggcc ggggcgacgg ggccttcaac gactcggcct acgcgggcct
      301 ggtccaggtc gcgaaggcct acggcgggaa gatcgtcggc gaccccgacc gcgtcgacca
      361 cggcgggaag atcgagctca ggtacctcga gtccaagctc ggcggccagg acagggagca
      421 gctcctccgc gtcctctccg aggagggcaa ccggctcgtc ttcggggtgg gcttcatgtt
      481 cgccgagtcg atcgcgcggg tggccaagga cttcccgaac gtccactacg ccctcgtcga
      541 cggcgtcgtg cccgacctcg cggagggctc gaacgtcacc tgcctcggct tcgcggagga
      601 ggaaggctcc ttcctggtcg gcgccctgtc cgggttggcg gtggccgaca agcccggcgc
      661 gaaggtcggc ttcatcggcg gcatggacac gcccctcatc caccgcttcc aggccggctt
      721 cgcggccggc gcggcctacg ccaatccgcg gctccgcaag cccggcatgg tcctaggcca
      781 gtacatcggc cgggacggct cggccttcaa cgacccgaag acggccgccg ccatcgcctc
      841 cacgatgtac aagggcggag cggagatcgt ctatcacgcc gccggcggct cgggagccgg
      901 cctcttcgag gccgcggccg ccgcgggcaa gctcgcggtc ggcgtggact ccgaccaggg
      961 ctattccctg atgaccgacg cgaaggaccc ggccgccagg gaactggggg ggcgggtcct
     1021 cacctcgatg ctcaagcgcg tcgaccaggc cgtcttcctc accgccaagg agctcgtgga
     1081 gagcggcaag gtcaggggcg gctaccgcac cttctccctc gccgacgacg gcgtcggcgt
     1141 ggcgaagaac gagttcaact cgggcaagct cgcgccctac gaggccgagc tcgacgccct
     1201 caaggccagg atcgtcgccg gggagatcaa ggtgcccgcc gacgcggagg ccgccgcggc
     1261 cttcgtgaag gggctcaagt agggggcggc cttccggccg ccttcggctc cgcgcctaaa
     1321 tggcatgagg gcgccgcgcg cgaaccgcgc gcggcgccct cttcgcatcc cgcgatgtcc
     1381 tcaggcggag cctacttggc ggagaagacc ttcgcggcct cggcgggata gttcgcgcgg
     1441 aggatggtct ccagggctcc ttcccgcgag agcgaggcgg cgagtcccgc catgaggccc
     1501 aggtggggga cctcttcccc cttcggcgag acgaggagaa ggacgacgcg ggccgggatg
     1561 ccgtcggcgg ccccgaagtc gatgccctgc tcgaagacgc ccaaggcggc cgcgggcttt
     1621 ttcgccgcgt cggagcggcc gtgggggaag gcgaccccgc gctccaggcc cgtcgcgccg
     1681 atgtgctcgc gggccagcac gtcgaccgcg gcctcctcct cgtcgccgat cttgcccgcg
     1741 gccttgagca cgccgaggag cttccggatc gcctcctcct tgtccctcgc cttgaggccc
     1801 agcttgacgc actcgggcgg gatgagggcc gtcttcgcgg tctcctccga ggaggccccg
     1861 acgaggccga tcctcaggga ctcggcgtcc agggacttgc ccgcgcgcct gaaggagtcg
     1921 gtgagctcga ggatcgtctc gtgggcggcc gccttggcca ggtagaggct cttcttcgag
     1981 gcctcgagca cgatgcccgc gccttcgggg cggatcacga tgcgggagtc ctcgcgggcc
     2041 gcgacgtaga tgtcggactc gggatcggcc aggcgcacga agaagccctc gtgcctgagc
     2101 tgggcggcca tggactcgac gacgagctcg acgatgccgg gatcctcgat gggaatctcg
     2161 atcgtgcccg tctccgagcc cgcctgcggc gtcttggtgc ccgcgcccgg cttggcgagg
     2221 aggagcttga ggaagggcgg aggcacgacg gtcgtgagga gggtcatcat gatcacgacg
     2281 ccgaagagct tctcgtccag gaagccgccc gagatgccga tgcccgcgat gatgagggcg
     2341 acctcgcctc gcggcaccat gcccacgccg acgcggaggg ccccgagggg gttgaagccg
     2401 aagaagaggc tcgggagggc gcagccgacg atcttggagc cgaaggcgac gaaggtgtag
     2461 accgcgccga aggccaggac ctcgggcgag gagagctcct tcaggtcgac gagcatgccc
     2521 atcaccgcga agaagatcgg gaccaggaac atgtagagcg gctggagctt ctcgcggatg
     2581 acgagcttca ggtccgtgcc cgagagggac aggcccatga tgtaggcgcc gatgatcatc
     2641 gcgaggccct ccgtctcgaa gaagccggcg aggaggaggg cgaggcctag ggccaggatg
     2701 gcgaaggcct ccggcgtgcg gaagcgcttg aggaagcggg cgatgcgcct ggagaaggcg
     2761 aggccgagga gggtgaagac ggcccagatg ccgaaggcct tcgcggcgat gccgcctatc
     2821 ctgccccagt tcaccgctcc cgagcccgag gagaggacgc tcgagatgcc caggaccacc
     2881 gccaggcaga tgatgccgag gacgtcgtcg atgacggcgg ccgcgaggat ggtcgtgccc
     2941 tcgggactgt ccatcttgcg ctggtcggag aggatccgcg cggtgatgcc gacgctcgtc
     3001 gcggtgccca ggatgccgag gaagaggctg cgcgggtcga gccaggtcca gtggaaggcg
     3061 aggacgccgc agagcgcgcc caggccgaag gaggcggcga cgccgcccag ccccacggcc
     3121 aggcccgccg ccgcgtagcg cttgaagagg gagatgtcgg tctccaggcc cgacatgaag
     3181 aggaggacga tggaggcgag ggtcgcgaag ccgtagagct cggtggagac ggcgaagccg
     3241 gaggcggcag ccgggaagag gcccgcggga aagccgggca gggcgacgcc gccgagggcg
     3301 ccctgcgaga ggaggatgcc cgcggcgagc tctccgagga ccgagggcag gcccaccttc
     3361 gaggcaaggc tccctccgag atgggcgacg atgatgatga ggccgagctg gatgacgagg
     3421 gacgtcatcg atgcggtgac gcctccggtc ccctcctcgg ccagggcggg gaggacgagg
     3481 gccagggcga agacaataag gaagaggaac ttgcggagct tcggtcttgg actcatggac
     3541 aaaccttgtg atggggtccc gtcggcaggg cggggctccg agatggcgga ggggcgaggc
     3601 cctggcgcca ccatcggcga aggccgaagg atggcccagt atgccgaatt ccgagcctga
     3661 gcttcaacgg ggcttgcgct ctgttgtatt actatagaaa aaggacgaaa agcctggaga
     3721 aagccgcggc gtgtccgagc cgccgcgcgc gctccgcttc gatgcctgtc cccggccatg
     3781 gcggaggtag cgcggcgctt ttcctgtaat gccggggacg ggcatcgaac ccgcacgcgc
     3841 cttgcggcgc aagggatttt aagtccctgg tgtctaccga ttccaccacc ccggcggagc
     3901 gcccggctgg ctggcggggc gtgaggctac tttacggtca ggcatgggaa gcgtcaatgc
     3961 ccgccccttg tcgcttcacc tcaaggggct ttcggagcta ggatggcgtc catggatagc
     4021 gactatctcc tcgccggggg acgcttcgtc cttccggagg ccgtcctcga cgggggagcc
     4081 ctcctggtgg agaagggccg catcgcgagg atctacgcgg cgggagagcc cctccccacg
     4141 gggccggcgc ggatcgacgc gaagggcgcc tgggtcaccc ccggcctcat ggagctccac
     4201 atccacggcg ctggaggagt cggcttcgag gctctcggcc tgggcgcttc gaagcgcgcc
     4261 ggcgccgggg cgggtccgga cgagatggac aggccgggcg agccggagag gacgggctat
     4321 gccgaggccg cggcgcggga cctggagggc gtcgccgtct tcctggaggc gcggggcgtc
     4381 accgccttcc tccccaccct ggtcgccgag cccaggtcgg cggagcgcct cgcgctggcg
     4441 ctggaaaccg cggcctttcc cgctcatcgc gtccccggga tctacatcga aggccccttc
     4501 gtcaatcctg cccgccgggg cggcctcccc caggaattcc tccgagaggt aggccccaag
     4561 atcctcgact cctacctcca ggcggcgcgc ggcaggctca aggtcatgac ctacgcgccc
     4621 gagcttcccg gcgccgatag ccttcccggg cgcctcgccg aggaaggcgt cctccccgcc
     4681 ctcggccatt ccgacgccgt cctcggccgc ttcgagccgc ctgaggggcc ctattcgctc
     4741 acccaccact tcaacgcgat gtcgcccttc tcgcacaagg agccggggct cgcgatgctg
     4801 cctttcctcg atcccaggcc ctcggtggag ctcgtctccg acggcgtgca cgtgaacgac
     4861 gaggccctga ggatctgcgc cgcggccttc gggtcgggac gaatcctcct catctccgac
     4921 gcctgcgcgg cagcaggcct tcccgacggc gagtacgagt acttcgggcg caggatcgtc
     4981 tccggggagc ggggggtgcg ctacgcggac tcgggcgtgc tcatgggttc gaggcgcctc
     5041 ggccccgagc tcttgcggaa ctggctccgg gcgaccggcc gtcccgtcca cgaggccgtc
     5101 ctcgccctga cgcgcaatcc ccggcggctc gtcggcgtct tggaccgcgg ctccatcgag
     5161 cccggccagg cggcggagct cgtcgtctgg gagggcgagt tcgaggcgcc gcgcctggtc
     5221 ctccgctagg cggcccgcgg ccggccccgg ccaggcctgg gccgtccccc ggccgcgccg
     5281 gccggggcgg aggcccaggc gacaccacca caaggagcgc gatatgcgat ctacgtgtct
     5341 gtacgaatcg gacgggggct cgccccgccg gatcctctgc ttcggcgact cgaacacctg
     5401 gggctacgag cccgggacgg gccggcgctt cgcggagggc gtccgctggc ccggcgtcct
     5461 cgcctcggag ctcggggccg gcttccgcgt cgtcgaggag gggctctgtt cgcggaccac
     5521 cgtcttcgag gatcccctgg ccgccggccg gcgcggaatc gaggccctgc cgacggctct
     5581 cgagagccac gcgcccctcg acctcgtcgt cctcatgctc ggcacgaacg acctcaagcg
     5641 ccgctacgcc tcgggcgcct acgagatcgc gctgggcctg gagcggctcg cgcgccttgt
     5701 cctctcctcc ggagcggggc gggcctcgcc ggacggaacg gccgcggagg ctccggccct
     5761 cctcctcgtc gcgccgccgc ccctcggcgc gctcggcgaa aacggcgccg ccttcgaggg
     5821 cggcagggcc gtctccctcg agctgcccgc gcgcgtcgcg gccctggcgg ccgccctcgc
     5881 ggctgagggc cgcgccgtcg gccatctcga cgcgggcgcc gccgccgccg ccggctccga
     5941 cggcatccac ctcgaccccg ccgcccacgc cgccctggg
//
LOCUS       DAOOSJ010000172         5977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8240_length_5977_cov_6.767477, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000172 DAOOSJ010000000
VERSION     DAOOSJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8240_length_5977_cov_6.767477"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLG14_08925"
     CDS             complement(<1..863)
                     /locus_tag="PLG14_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOX49292.1"
                     /translation="MATTYGYTGRILRVNLTTGKVSTDDTLKYKDFIGGEGMAMKIMW
                     DEVAPGVKPYDPENKLVFGTGPLCGTGVPCSGRTNITSLLPSSPFHGVSSSHMGGYFA
                     TELKYAGWDLIVVEGKAVHPVWLRIEDDKVRIEDARWMWGKGIFDTQAMVASFMGKEA
                     QVAAIGQAGENLVNLSVIRTGSSHSAGGHGGVMGSKNLKAIGVKGSGSVKIAGDKQAW
                     AELDKLMMSVIGANNQMVVPSTPQPWAEYSDKSSRWTGRKGLFWGAANPPVETGECDP
                     HDMQSVGFRTM"
     gene            complement(882..1571)
                     /locus_tag="PLG14_08930"
     CDS             complement(882..1571)
                     /locus_tag="PLG14_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX49293.1"
                     /translation="MSDSTEKREGILDKDQTRRDFLKMTGTGLAGAFVALTLGCATTV
                     RTREGNELRVWATPQGALVHDPNRCVGCKRCEAACTVRNDGKASAHLSRIKVSRNLNF
                     GVAGVSAAYAKADGQLGNFRVVAETCRQCEKPACGIACPAGAIGTDKKTGARVVNAKL
                     CVGCGACHDACPWNLPTVDAETRKSTKCLVCDGDPSCAKACPTGAIRYLPYAEAEKLL
                     WGAEVVSGATA"
     regulatory      complement(1636..1795)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     assembly_gap    1848..1947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1948..3893)
                     /locus_tag="PLG14_08935"
     CDS             complement(<1948..3893)
                     /locus_tag="PLG14_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX49294.1"
                     /translation="MKLVEAIGLSKDWAGTPVFEGAAFSIEEGEKIGLVGRNGCGKSS
                     LLGVLAGRDEDYRGTLRRKPGLRAALVPQAYEPPAGLACAALLAEPAARLALRLEALG
                     EELGSLEGSALERALAEYGELRGRYELLGAEGAEEKAERLLERLGLPGSAGREAARLS
                     GGEKNVLALALALMGEPELLLLDEPGNHLDFAGLSWLEEFVRGERRAVLMVSHNRWLL
                     DRSVDSILELEERRVSRYSGGFSAFRLEKLKRAAGQGKEWQADRKRVERLEALVRRFA
                     EIAAARPDPAWGKRLRARRSQLEREKAQAAERPELGGAKMSLSFAGAESKADFALVVR
                     GYSKAFGSRVLFDGAGLDLLVGERAALVGPNGSGKTSFVRDLVAAAGSLDGGPIRVGP
                     SMVLGYCAQERELFRAERTVAEEFGALGARDAETAKLLRRFLFGREVLEKRIASLSGG
                     ELNRLQIARAVYLGANFLVLDEPTNHLDIEGREAVEEGLADFEGTVLVVSHDRWFLEK
                     VAERIILVEDGKLLPYEGSFAEYWRDRGASAAPRSSRPAVSRALEDRGAELSRAREAR
                     GRAAGGAASGAASRTPSGAPPGAAKGETARAAKGAASAAASRAAALEARIGELEKEKL
                     RAEGESARAIGRRDFERGGRLAEEA"
     gene            complement(4213..4686)
                     /locus_tag="PLG14_08940"
     CDS             complement(4213..4686)
                     /locus_tag="PLG14_08940"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HOX49295.1"
                     /translation="MSDPILGLPEGLAEFLLKAKRRTYAAGAPPTASSRPSSKDLGWA
                     EGGFSYLDSYFGSRDFLGEEVVLFQGKPVWGMNYHGLSLAPEAELEGMVPCLLGALME
                     VPLEAPYRGPARFSLGDCEYRCRWEGGLGSFLGEEEIRKGGRLVYTLRFHGGAID"
     gene            4763..>5977
                     /locus_tag="PLG14_08945"
     CDS             4763..>5977
                     /locus_tag="PLG14_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOX49296.1"
                     /translation="MELAEKLEILAAGARYDASCASGGSSRGPETGARLGAASPAGVC
                     HSFAGDGRCVSLLKLLYSNVCRYDCAYCLNRSSNDQRRCSFDPEELVRLTVSFFRRNY
                     VEGLFLSSGIFSDPDIVMERLVEVARLLREREGFGGYIHLKAIPGCGPKILRKAGLYA
                     DRLSANIELPTAESLARLAPDKSGAEILGAMRFIGEACAEEREDRRRLASTPRFAPAG
                     QSTQLVVGASPEDDRSVLGLAEGLYRRYGMRRVYYSAFVPVRPDPRLPGTPPPLLREH
                     RLYQADWLLRFYGFRAGELLDAAGPSLPSDLDPKCAWALAHPEFFPVELSRAELPALL
                     RVPGLGLTSARRIVATRRTGSLREADLPRIGLVLKRARYFLSLHGRRLAEPPEPGLLR
                     ALLSDQAELAPAA"
BASE COUNT          801 a         2130 c         2048 g          898 t
ORIGIN      
        1 ttcatcgtgc ggaagcccac ggactgcatg tcgtgcgggt cgcactcgcc ggtctccacg
       61 gggggattcg cggcgcccca gaagaggccc ttgcgcccgg tccagcggga ggacttgtcg
      121 ctgtactcgg cccagggctg gggggtggag gggacgacca tctggttgtt ggcgccgatg
      181 acgctcatca tgagcttgtc gagctcggcc caggcctgct tgtcgcccgc gatcttcacg
      241 gagcccgagc ccttgacgcc gatcgccttg aggttcttgg agcccatgac gccgccatgg
      301 cctcccgcgg agtgcgagga gcccgtgcgg atgacggaga ggttgacgag gttctcgccg
      361 gcctggccga tcgccgcgac ctgggcctcc ttgcccatga agctcgcgac catcgcctgg
      421 gtgtcgaaga tgcccttgcc ccacatccag cgggcgtcct cgatcctgac cttgtcgtct
      481 tctatgcgga gccagacggg atggaccgcc ttgccctcga cgacgatgag gtcccagccc
      541 gcgtacttga gctccgtggc gaagtagccg cccatgtggg agctggagac gccgtggaag
      601 gggctggagg ggaggaggga ggtgatgttc gtgcggcccg agcaggggac gccggtgccg
      661 cagagggggc cggtgccgaa gacgagcttg ttctcggggt cgtagggctt gacgccgggg
      721 gcgacctcgt cccacatgat cttcatcgcc atgccctcgc cgccgatgaa gtccttgtac
      781 ttgagcgtat cgtcggtgga gaccttgccc gtcgtgaggt tcacgcggag gatcctgccg
      841 gtgtatccgt aggtagtagc catgttcgtt tcctccttgc cttacgccgt cgcgccggag
      901 acgacttccg cgccccagag gagcttttcg gcctccgcgt aggggaggta gcggatcgcg
      961 cccgtgggac aggccttcgc gcaggaagga tcgccgtcgc agacgaggca cttcgtggac
     1021 ttgcgggtct cggcgtccac cgtcgggagg ttccaggggc aggcgtcgtg gcaggcgccg
     1081 cagcccacgc agagcttcgc gttcacgacg cgggcgccgg tcttcttgtc cgtgccgatg
     1141 gcgccggccg ggcaggcgat gccgcaggcg ggcttctcgc actggcggca ggtctccgcg
     1201 acgacgcgga agttgccgag ctggccgtcg gccttggcgt aggcggcgga gacgccggcc
     1261 acgccgaagt tgaggttgcg ggacaccttg atgcgggaga ggtgggcgga ggccttgccg
     1321 tcgttgcgga cggtgcaggc ggcctcgcag cgcttgcagc ccacgcagcg gttcgggtcg
     1381 tggacgagcg cgccctgggg ggtggcccag acccgcagct cgttgccctc gcgggtgcgg
     1441 accgtggtcg cgcagcccag ggtcagggcg acgaaggcgc cggccaagcc ggtgcccgtc
     1501 atcttgagga agtctcgacg ggtctggtcc ttgtcgagga tgccttcccg cttctccgtg
     1561 gaatcgctca tcgcttctgc ctccggttcg ccggcctgga gcggggccca gaggggcccg
     1621 ccccctgccg tagggggatc gatgatcgtt tctcctttcc aatgccggaa gaggcgggag
     1681 gcggaggggt ttccccccgc gcctttaagg ccgcgggcga cgcccgcgcc tcaagaccgc
     1741 cggccatcgc ctgggagagg cgtccaggcc gggcggttcg gagtacgatg aaaaagatcg
     1801 gtcagtgccc ttgcgtgacg ctcgggccta tggcgtcatc ttaggaannn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc tcctcggcga gccgccctcc cctctcgaag
     1981 tcgcggcggc cgatcgccct cgccgattcg ccctcggccc tgagcttctc cttctcgagc
     2041 tcgccgatcc gcgcctccag cgcggcggcc cgcgaggccg ccgccgaagc cgcgcccttg
     2101 gccgcccgcg cggtctcgcc cttggccgcg cccggcggcg cgccggaagg cgtccgcgaa
     2161 gccgcgccgg aagccgcgcc cccggcggcc cggccgcggg cttccctggc gcgggacagc
     2221 tcggcgcccc tgtcctccag ggcgcggctt accgcgggtc gcgaggaccg cggagctgcg
     2281 gaggctccgc gatcgcgcca gtactccgcg aagctcccct cgtagggcag gagcttgccg
     2341 tcctccacga ggatgatccg ctcggcgacc ttctccagga accagcggtc gtgggagacg
     2401 acgaggaccg tgccctcgaa gtcggccagt ccctcctcca ccgcctcccg gccctcgatg
     2461 tcgaggtggt tcgtcggctc gtcgaggacg aggaagttcg cgcccaggta gaccgccctc
     2521 gcgatctgca ggcggttgag ctcgccgccg gagagggagg ctatccgctt ctcgaggacc
     2581 tcgcggccga agaggaagcg gcggaggagc ttcgccgtct ccgcgtcgcg cgcccccagg
     2641 gcgccgaact cctcggcgac cgtccgctcc gccctgaaga gctcccgctc ctgggcgcag
     2701 tagcccagga ccatcgaggg gccgacgcgg atgggcccgc cgtccaggct gcccgccgcg
     2761 gcgacgaggt cgcgcacgaa gctcgtcttc cccgagccgt tggggcccac gagggccgcc
     2821 ctctccccca cgaggaggtc gagcccggcc ccgtcgaaga ggacgcggct tccgaaggcc
     2881 ttggagtagc cccggacgac gagggcgaag tcggccttgg actcggcgcc cgcgaaggag
     2941 aggctcatct tggccccgcc cagctccggc cgctcggcgg cctgggcctt ctctcgctcg
     3001 agctggctcc gccgggcccg gaggcgcttc ccccaggcgg gatcgggccg cgcggcggcg
     3061 atctccgcga agcgccgcac cagggcctcc aggcgctcga cccgcttgcg gtcggcctgc
     3121 cattccttgc cctggccggc ggcccgcttg agcttctcca ggcggaaggc cgagaagccg
     3181 cccgagtagc gcgagacgcg ccgctcctcg agctcgagga tggagtccac ggagcggtcc
     3241 aggagccagc ggttgtgcga caccatgagg acagcccgcc gctcgccccg gacgaattcc
     3301 tcgagccagg agaggcccgc gaagtcgagg tggtttcccg gctcgtccag gagaaggagc
     3361 tcgggctcgc ccatgagggc cagggcgagg gccaggacgt tcttctcccc gccggagagc
     3421 cgggccgcct ctcggcccgc cgatcccggg aggccgaggc gctcgaggag gcgctccgcc
     3481 ttctcctccg ctccctccgc cccgaggagc tcgtagcggc cgcggagctc cccgtactcg
     3541 gcgagggccc gttccagggc tgagccctcc aggctcccga gctcctcgcc cagggcctcg
     3601 agcctcagcg cgagcctggc cgcgggctcc gcgaggaggg cggcgcaggc caggccggcc
     3661 gggggctcgt aggcctgggg cacgagggcc gctcggaggc cgggcttccg cctcagggtc
     3721 ccgcggtagt cttcgtcccg acccgccagg acgccgagga ggctcgactt gccgcaaccg
     3781 ttcctcccga ccaggccgat cttctccccc tcctcgatcg agaaggccgc tccctcgaag
     3841 acgggcgtgc ccgcccagtc cttggagagg ccgatcgctt ccaccagttt catcttcgct
     3901 ccttcctgcg gcgagagcgg cttccttgga ggagggcgcc gcgcggcgct ccgggcccgc
     3961 ccgccgcagg tccatgcgcg gactggcggc gggcataaaa aaagcgcggc ctcccgtcaa
     4021 ggaaggccgc gcgtcgctcg ctatggatta tctcaaatat ctccaagcag ggcttcgggc
     4081 agaccttccc cgtcggagag agccgcaccg gtagagcgcc gcccgtggac gaggatggcc
     4141 gaacgaaggt cccgcttgag catgggccga tcctacaggg cggagaccgc ggccgtcaag
     4201 ctagtcgtca agctagtcga tggcgccgcc gtggaagcgc agggtataga cgagcctgcc
     4261 gcccttccgg atctcttcct cgcccaggaa ggagccgagg ccgccttccc agcggcagcg
     4321 gtattcgcag tcgccgaggg aaaaacgcgc cgggccgcgg tagggagcct caagggggac
     4381 ctccatgagg gccccgagga gacaggggac catgccctcg agctcggcct cgggagccag
     4441 ggagaggccg tggtagttca tgccccagac cggcttgccc tggaagagga cgacctcctc
     4501 gcccaggaag tccctggagc cgaagtagct gtcgaggtag ctgaaaccgc cctcggccca
     4561 gcccaagtcc ttcgaggaag gccgggagga ggccgtcggc ggggcgccgg ccgcgtaggt
     4621 gcggcgcttg gccttgagga ggaactcggc gaggccctca ggaaggccca gtatcggatc
     4681 gctcatggaa tcccctttcc gcgccggcct ttttcccgga cggcgcttgc ggatactagc
     4741 acacgtctgt atagttgtcc tcatggaact cgcggaaaaa ctggaaatcc tcgccgcggg
     4801 agcccgctac gacgcctcct gcgcctcggg cggcagttcg aggggcccgg agacgggggc
     4861 gaggctcggc gccgcgagcc cggcgggcgt ctgccattcc ttcgcggggg acggcagatg
     4921 cgtctccctc ctcaagctac tctattccaa cgtctgccgc tacgactgcg cctattgcct
     4981 gaaccgctcc tccaacgacc agcgccgctg ctccttcgac ccggaggagc tcgtccgcct
     5041 gaccgtctcc ttcttcaggc ggaactacgt cgaaggcctc ttcctctcct ccggcatctt
     5101 ctccgatccc gacatcgtga tggagcgcct ggtcgaggtg gcccgcctcc ttcgggagcg
     5161 cgagggcttc ggcggctaca tccacctgaa ggccatcccc ggctgcgggc ccaagatcct
     5221 caggaaggcc ggcctctacg ccgaccggct ctccgcgaac atcgagctgc cgacggcgga
     5281 aagcctggcg cgcctcgccc ccgacaagtc gggcgcggag atcctgggcg cgatgcgctt
     5341 catcggcgag gcctgcgccg aggagaggga ggacaggcgc cgcctggcct cgaccccgcg
     5401 cttcgccccg gcaggccaga gcacccagct cgtcgtcggc gcgagccccg aggacgaccg
     5461 gagcgtcctc ggcctggccg aggggctcta taggcgctac gggatgcgcc gcgtctacta
     5521 ttcggccttc gtccccgtcc gacccgaccc gcggctgccg ggcactcccc cgcccctcct
     5581 ccgcgagcac cgcctctacc aggccgactg gctcctgcgc ttctacggct tccgggcggg
     5641 cgagctcctc gacgccgccg ggccgagcct gccttccgac ctcgacccca agtgcgcctg
     5701 ggccctggcc caccccgagt tcttccccgt cgagctctcc cgcgccgagc tccccgccct
     5761 cctgcgcgtg ccgggcctgg gcctcacgag cgcgcgcagg atcgtcgcga caaggaggac
     5821 gggctccttg cgcgaagccg acctgccccg catcggcctt gtcttgaagc gcgcccgcta
     5881 cttcctcagc ctccacggcc gccgcctggc cgagcccccc gagcccggcc tcctcagggc
     5941 actcctctcc gaccaggccg agctcgcgcc ggcggcc
//
LOCUS       DAOOSJ010000173         5967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8256_length_5967_cov_6.706698, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000173 DAOOSJ010000000
VERSION     DAOOSJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8256_length_5967_cov_6.706698"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PLG14_08950"
     CDS             <1..1163
                     /locus_tag="PLG14_08950"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOX49297.1"
                     /translation="GATVTGAREFLIAYNVNLNTRDKKLANEVALTIREGGRAAKDAK
                     GNTLKDAAGNTVKVPGLLKDVRAIGWYIDAYKCAQISINLVNYRTTPLHVVFETAKAE
                     AEKLGLLVTGSEVVGLLPLEPLLDAGRHFLRKMGKSEGVSETELVETAIRSMGLDSVA
                     PFEPSKKIVEYAGRQPAPLVSMKVADFVDEVASESPAPGGGSVAALSGSLGAALAAMV
                     ANLTVGKKGYEAAWAEMSALAVEGQRVKDALVRAVDEDTAAFNDLMEAMKLPKATDAQ
                     KAARDAAMQEGYKKAAAVPLETARVCVEAISLCERAAAKGNANSASDAGVGALLARAG
                     AEGAALNVLINLGSITDAAFVADLKARAQALVAEAEAAKEKVLALVGKSIGA"
     gene            1189..1851
                     /locus_tag="PLG14_08955"
     CDS             1189..1851
                     /locus_tag="PLG14_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49298.1"
                     /translation="MKQGAEYLATVLARAAGSVHLPRALRGAGGPFLLHVSDPPSSLY
                     PLVGRLLDALRPAWLLLTGDLADELKLELYPALRHRYAARLADLREVLLPRLAAAPRG
                     GEPGQGLRLLVCAGNHDDALLVRSLFPEALVVGDSLRFRAGGLDCAASHWAARAEETG
                     ADYLFYGHDLSRPSGEEGRRRFLNGVSSINVVDLGSGGVFHLPYPAGTDAKRLLRPKI
                     GL"
     gene            1912..2916
                     /locus_tag="PLG14_08960"
     CDS             1912..2916
                     /locus_tag="PLG14_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49299.1"
                     /translation="MLSFMRGGPRLLVAESPDGALADWLGRQGSESPSFEEAFDAGDG
                     ESCIVGLVDSVGVPVRLADVRRLFLVHLPVEDLLLRMLSDKAPGRGRVRMAPRLLVIR
                     GAGDLGAVVGRIEDEIGAFPGRFLDLVDEAEDGVILSVTARSLESKVALADLHPVSLH
                     VPRSALEPRELERLLRLNGLRYLNAGLGDRDWYDLEIRVYDRYEAYGIQLERILRIAN
                     ALELGLVLGESWSKDSPRFLMSIDVYRIRLASYMVPERVKGFLLGLEYLDDGLRVGDY
                     DLMRGDRKVHWSDAAVEGIRDRKELGMRFRRELLGLLPQADREALSLLEDRLRASRVD
                     "
     gene            3105..4283
                     /locus_tag="PLG14_08965"
     CDS             3105..4283
                     /locus_tag="PLG14_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49300.1"
                     /translation="MKGSDVAVSALRKSFGGFEALKDVSLEIRKGEFFSLLGPSGCGK
                     TTLLRVIAGFEDPDTGIVAIDGREVNGLPPDKRRCNTVFQNYALFPHLSVFENVAFPL
                     RLRKVPNRLVKEQVRRHLALVQLESHAGKKPSQLSGGQKQRVAIARALINEPSVLLLD
                     EPLSALDAKLRQHMLLELDQIHDQVGITFIYVTHDQAEALSVSDRIAVMDGGRVLQVG
                     SPQEIYENPKSEFVARFIGDTNLFKGLVVAREGPIARVEAEGYGPLLVEANGEAPVGA
                     RVTVAVRPEKLRIASAPPPRHAEANVFRGVVEEPVYSGFQTKYLVRGSASAAGEAELA
                     GSGPSSSLSVLRQHAQWSEGVPDIRWKDEVYLYWSARDAVVVETDARGGKPPEAEAPR
                     "
     gene            4280..5146
                     /locus_tag="PLG14_08970"
     CDS             4280..5146
                     /locus_tag="PLG14_08970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49301.1"
                     /translation="MSAKRNYGALYAAPQGLWLLAFFAAPLAIIVAYSFMRKGLYGGV
                     TGELSLDAYRALANPSLLAVAARTLAVSLAATALTLLIALPCGYHIARSRNQALRLFL
                     VIVPFWTNFLVRIYAWIAILGNEGFLNDFLRALGVVADPVQFLYNQWAVVLVLVYMYL
                     PYAILPLFSTIDKFDFTLLEAARDLGATKPQAMGRVLLPNIQGGVVTAVLFTFIPIFG
                     AYAVPLLVGGKDSYMLGNIIADQVTKTRNWPLASAISMTVTALTGVAALAFLYRSRKP
                     APAPLEGQGGAS"
     gene            5143..5931
                     /locus_tag="PLG14_08975"
     CDS             5143..5931
                     /locus_tag="PLG14_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX49302.1"
                     /translation="MKARRKLSFSALVYWSTIAFLFLPLAVLLVYSFNSSKSGTWTGF
                     SLVWYEKLVFGSPELWKAFANSLVVALGSASAATLLGTLAAVGTNRFRFRGRAAVQAL
                     SFVPMILPEIVVGVSLLVFFAGVGLKLGMATVLIAHTTFNLPFVFLLVSARLEEFDSS
                     IVEAARDLGANERQTLFRVIVPMALPGVASGFLTAVTLSLEDFVITFFVSGPGSTTLP
                     LYIYSMIRFGVSPVINALSAVMVAGTIALVYPLRNFLKTFAAHS"
BASE COUNT          820 a         2320 c         1883 g          944 t
ORIGIN      
        1 ggggcgccac cgtcaccggc gcccgcgagt tcctcatcgc ctacaacgtg aacctgaaca
       61 cccgggacaa gaagctcgcg aacgaggtgg ccctcacgat ccgcgagggc ggccgcgccg
      121 cgaaggacgc gaagggcaac accctcaagg acgccgccgg caacaccgtg aaggtgcccg
      181 gcctcctcaa ggacgtgcgc gccatcggct ggtacatcga cgcctacaag tgcgcgcaga
      241 tctccatcaa cctcgtcaac tacaggacga cgcccctcca cgtcgtcttc gagacggcga
      301 aggcggaggc cgagaagctc ggcctcctcg tcacgggcag cgaggtcgtc ggcctcctcc
      361 ccctcgagcc cctcctggac gcgggccgcc acttccttag gaagatgggg aagagcgagg
      421 gcgtctcgga gaccgagctc gtggagacgg cgatccgctc catgggcctc gactccgtcg
      481 cgcccttcga gccttcgaag aagatcgtcg agtacgcggg ccgccagccg gcccccctcg
      541 tctccatgaa ggtggccgat ttcgtggacg aggtcgcgag cgagtccccc gcccccggcg
      601 gcggctcggt ggcggccctc tcgggctccc tgggcgccgc cctcgcggcg atggtcgcca
      661 acctcaccgt cggcaagaag ggctacgagg ccgcctgggc ggagatgtcc gccctcgccg
      721 tggagggcca gcgcgtgaag gacgccctgg tccgcgcggt ggacgaggac acggccgcct
      781 tcaacgacct catggaggcc atgaagctcc cgaaggcgac ggacgcgcag aaggccgccc
      841 gcgacgcggc gatgcaggag ggctacaaga aggccgcggc cgtgcccctg gagacggccc
      901 gcgtctgcgt cgaggcgatc agcctctgcg agcgggccgc cgcgaagggc aacgccaact
      961 ccgcctcaga cgcgggcgtc ggcgccctcc tcgcgcgcgc cggcgccgag ggcgccgccc
     1021 tcaacgtcct catcaacctc ggctccataa ctgacgccgc cttcgtcgcc gacctcaagg
     1081 ccagggccca ggccctcgtg gcggaggccg aggcggccaa ggagaaggtc ctcgccctcg
     1141 tgggaaaatc gataggcgcc tagggcctgg ccgggctata ctgccgccgt gaagcaaggc
     1201 gccgagtacc tcgccaccgt cctggcccgc gccgcgggct cggtccacct gccccgcgcc
     1261 ctgcgcgggg cgggcgggcc cttcctcctc cacgtctcgg acccgccgag ctccctctat
     1321 cccctcgtcg gccgactcct cgacgccctg cggcccgcct ggctcctcct cacgggcgac
     1381 ctcgcggacg agctcaagct cgagctctac ccggccctga ggcaccgcta cgccgcccgc
     1441 ctcgccgacc tgcgcgaggt cctcctccct cggctcgcgg cggcgccgcg ggggggcgag
     1501 ccgggccagg gcctccgcct cctcgtctgc gccggcaacc acgacgacgc cctcctcgtg
     1561 aggagcctct tccccgaggc cctggtcgtc ggggactccc tgcgcttccg cgccggcggc
     1621 ctcgactgcg cggcgagcca ctgggccgcc cgcgcggagg agaccggcgc cgactacctc
     1681 ttctacggcc acgacctctc gaggccgagc ggggaggagg gccgccgccg cttcctgaac
     1741 ggcgtctcgt ccatcaacgt cgtggacctc ggctcgggcg gggtcttcca cctgccctat
     1801 cccgcgggga ccgacgcgaa gcggctcctg cgcccgaaga tcggtctgta gcctcccgtc
     1861 ttcgcgcggg aggccggggc gggccccgag gcggacccgc gaggaggaag gatgctttcg
     1921 ttcatgcgcg gcgggccccg cctgctcgtg gccgagtccc ccgacggggc cctggccgac
     1981 tggctcggcc gccagggctc ggagagcccg agcttcgagg aggccttcga cgccggcgac
     2041 ggcgagagct gcatcgtcgg cctcgtggac tccgtcggcg tcccggtgcg cctggccgac
     2101 gtgcgccgcc tcttcctcgt ccacctgccc gtggaggatc tcctcctcag gatgctctcg
     2161 gacaaggcgc cggggcgggg gagggtcagg atggcgcccc gcctcctcgt catccgaggg
     2221 gcgggcgacc tgggcgccgt ggtgggccgc atcgaggacg agatcggcgc cttcccgggc
     2281 cgcttcctgg acctcgtgga cgaggcggag gacggcgtca tcctctccgt caccgcgcgg
     2341 agcctggagt ccaaggtcgc gctggccgac ctccatcccg tctccctcca cgtgccccgc
     2401 tccgccctgg agccccggga gctcgagcgc ctcctccgcc tcaacggcct caggtacctc
     2461 aacgcgggcc tgggggacag ggactggtac gacctggaga tcagggtcta cgaccgctac
     2521 gaggcctacg gcatccagct cgagcgcatc ctgcggatag cgaacgccct ggagctcggc
     2581 ctggtcctgg gcgagtcctg gagcaaggac agcccgcgct tcctcatgtc catcgacgtg
     2641 taccgaatcc gcctcgcgag ctacatggtg cccgagcgcg tgaagggctt cctcctcggc
     2701 ctcgaatacc tggacgacgg cctcagggtg ggcgactacg acctcatgcg cggcgaccgg
     2761 aaggtccact ggtcggacgc cgcggtggag ggcatccgcg accgcaagga gctcggcatg
     2821 cgcttcaggc gcgagctcct gggcctcctg ccgcaggccg accgcgaggc cctctccctc
     2881 ctcgaggaca ggctccgcgc ctcccgcgtc gactaggccg cgactaggcg ccctccgcgg
     2941 cgcaaggtct ccagccgcgg ccaagccccc gccgccgcgc cgccccgccc gccggatgga
     3001 tatgcggcgg ggcggaatat ttatgcgcat ttggtcttga ccaaccgagc ctcttgtcct
     3061 tataccttgc gcatgatcga tttccgttag ggaggctccc tgaattgaaa ggttctgacg
     3121 tcgccgtttc ggcgcttcgg aagtccttcg gcggcttcga ggccctgaag gacgtttctt
     3181 tggagatcag gaagggcgag ttcttctccc ttctcggtcc ctcgggctgc ggcaagacga
     3241 ccctcctcag ggtcatcgcc ggcttcgagg atcccgacac cggcatcgtg gccatcgacg
     3301 gccgggaggt caacggcctc ccccccgaca agcggcgctg caacaccgtc ttccagaact
     3361 acgccctctt tccccacctc tccgtcttcg agaacgtcgc cttccccctg cgcctccgga
     3421 aagtcccgaa ccgcctcgtc aaggagcagg tgcggcgcca cctggccctc gtccagctcg
     3481 agtcccacgc ggggaagaag ccctcccagc tctccggcgg ccagaagcag cgcgtggcga
     3541 tcgcccgggc cctcatcaac gagccctccg tcctcctcct cgacgagccc ctctccgccc
     3601 tggacgcgaa gctcaggcag cacatgctcc tcgagctcga ccagatccac gaccaggtcg
     3661 gcatcacctt catctacgtc acccacgacc aggcggaggc cctctccgtc tccgaccgca
     3721 tcgccgtcat ggacgggggc cgcgtcctcc aggtgggcag cccccaggag atctacgaga
     3781 accccaagtc cgaattcgtc gcccgcttca tcggcgacac gaacctcttc aagggcctcg
     3841 tcgtcgcgcg cgagggcccc atcgcccggg tcgaggcgga gggctacggc cccctcctcg
     3901 tggaggcgaa cggcgaggcg ccggtcgggg cccgggtcac cgtcgcggtc aggcccgaga
     3961 agctcaggat agcttcggcc ccgccgcccc gccacgcgga ggccaacgtc ttccgcggcg
     4021 tggtagagga gcccgtctac tcgggtttcc agacgaagta cctggtccgc ggctcggcct
     4081 ccgcggccgg cgaggccgag cttgcgggct caggcccgag ctcgagcctc tcggtcctca
     4141 ggcagcacgc gcagtggtcg gagggcgtgc ccgacatccg ctggaaggac gaggtctacc
     4201 tttactggag cgcccgcgac gccgtcgtcg tggagacgga cgcgcgcggc ggcaagcccc
     4261 ccgaggccga ggccccgcgg tgagcgcgaa gcgcaactac ggcgccctct acgctgcgcc
     4321 ccagggcctc tggctcctcg ccttcttcgc cgcccccctc gcgatcatcg tcgcctactc
     4381 cttcatgagg aaggggctct acggcggcgt gacgggcgag ctctcgctgg acgcctaccg
     4441 cgccctcgcg aacccctccc tcctcgccgt cgcggccagg accctcgcgg tctccctggc
     4501 ggcgaccgcc ctcaccctcc tcatcgccct gccctgcggc taccacatcg ctcgcagccg
     4561 gaaccaggcc ctgaggctct tcctcgtgat cgtccccttc tggacgaact tcctcgtgcg
     4621 gatctacgcc tggatcgcga tcctgggcaa cgagggcttc ctcaacgact tcctccgggc
     4681 cctcggcgtc gtggccgatc ccgtgcagtt cctctacaac cagtgggccg tcgtcctcgt
     4741 cctcgtctac atgtacctgc cctacgcgat cctccccctc ttctcgacga tcgacaagtt
     4801 cgacttcacc ctcctggagg ctgcgcgcga cctgggcgcc accaagcccc aggcgatggg
     4861 ccgcgtcctc ctccccaaca tccagggcgg cgtcgtcacg gccgtgctct tcaccttcat
     4921 cccgatcttc ggcgcctacg ccgtccccct cctcgtcggc ggcaaggact cctacatgct
     4981 cggcaacatc atcgccgacc aggtgacgaa gaccaggaac tggccgctgg cctccgcgat
     5041 ctccatgacg gtcaccgccc tcacgggcgt ggccgccctg gccttcctct accgctcgcg
     5101 gaagccggcc cccgcccccc tcgagggcca gggaggcgcc tcgtgaaagc caggcggaag
     5161 ctctccttct cggccctggt ctactggtcg actatcgcct tcctcttcct ccccctcgcc
     5221 gtcctcctcg tctactcctt caactcctcc aagagcggga cctggaccgg cttctccctg
     5281 gtctggtacg agaagctcgt cttcggctcg cccgagctct ggaaggcctt cgcgaacagc
     5341 ctcgtcgtcg ccctgggctc ggcctcggcc gcgaccctcc tcggcaccct cgcggccgtc
     5401 ggcacgaacc gcttccgctt ccggggccgc gcggccgtgc aggccctgag cttcgtgccg
     5461 atgatccttc cggagatcgt cgtcggcgtg tccctcctcg tcttcttcgc cggcgtgggc
     5521 ctcaagctcg ggatggcgac cgtcctcatc gcgcacacga ccttcaacct gcccttcgtc
     5581 ttcctcctcg tctccgcgcg gctcgaggag ttcgactcct ccatcgtgga ggcggcgcgc
     5641 gacctcgggg cgaacgagag gcagaccctc ttccgggtga tcgtgcccat ggccctgccg
     5701 ggcgtcgcct cgggcttcct caccgcggtg accctctccc tggaggactt cgtgatcacc
     5761 ttcttcgtgt cggggcccgg ctcgacgacc ctcccgctct acatctactc gatgatccgc
     5821 ttcggcgtgt cgcccgtgat caacgcgctc tccgccgtga tggtggcggg cacgatagcc
     5881 ctcgtatatc ccttgcgcaa tttcctcaag accttcgccg cccattccta gggcgcgaag
     5941 gccgcttcgc cggaaggcgg cccctgg
//
LOCUS       DAOOSJ010000174         5861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8471_length_5861_cov_3.743713, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000174 DAOOSJ010000000
VERSION     DAOOSJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_5861_cov_3.743713"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLG14_08980"
     CDS             complement(<1..183)
                     /locus_tag="PLG14_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX49303.1"
                     /translation="MNQSGRPRALVVGTGFIGPAHLEALRRLGVPVAGLVERDAETAA
                     RKARELGIERAYPSYEA"
     gene            complement(206..1438)
                     /locus_tag="PLG14_08985"
     CDS             complement(206..1438)
                     /locus_tag="PLG14_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOX49304.1"
                     /translation="MKEREEGAGAGPSTSAGRIIRIIRDHGPLSPADIARRSGFAKST
                     VSLYVEKLLSVGLVREETKSGGKRRTLKVAESAGYVVGVDLGQTHLNVALCDLEADVL
                     DLSAGRVDFSREGPEAVLSRIAEAARSLAAKNGLSPSSLFGVGVGLPGPVDYQHGVPV
                     SPPVMPGWDRFPVASQLAREFTCPVFVDNDVNVMALAEREKGAAAGERDFMLVKAGSG
                     IGAGIVVDGKIYRGAKGAAGDIGHIGIDGVETLCRCGNHGCLEAVAGGKALALLADEA
                     ARSGASPFLAAQLAAGEAPSPELLALGAAHGDEECLRVVIAAGKALGNVLAKLVNFFN
                     PSLIVVGGGLSDLGERYLASIRESIYRRSTPLATSDLVVKRSALRERAGVVGAAVLVL
                     DEVFSQNNVGRLMRDIAP"
     gene            complement(1582..2553)
                     /locus_tag="PLG14_08990"
     CDS             complement(1582..2553)
                     /locus_tag="PLG14_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX49305.1"
                     /translation="MKKILATLLVLVAVTGLAFGQAQATKKITIGVTMPTADHGWMGG
                     ANWWAKRAMDDWKKKDPNVTFLFRTAPDVAKQVADIEDLIVQGVNGLVVFPHESAALT
                     PVVEKAYAKGIYVVVLDRGVTKPVYDVYLSNDDEAYARNSMEWICKELGYKGNLVLIE
                     GVPSVISDLRTKVAKDVAAKYPGIKVLDSQPGYWNKEKALAVMENYLQKYKQIDAVYT
                     ADDDMLEGVLQAYKESGRKDVKIMFGGAASKSMIKRIMDGDPLVRANATYPPDVCATA
                     VSMAVVGLRGRVFEGFYQKKLPIRIILNSEIITRENAKSYYVPESVF"
     gene            complement(2616..3584)
                     /locus_tag="PLG14_08995"
     CDS             complement(2616..3584)
                     /locus_tag="PLG14_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49306.1"
                     /translation="MTTIRKAPRLDFERFGSLVALAVLAAASAALSPHFLQPQNLLNI
                     LRQVSYTGIIGLGMTFVITAGGIDLSVGSLAAFTGSLAVLAMNAAAAKGLSEPSGVAV
                     AVAVALFVGAAGGAANGLLVTKGRIAPFIVTLGTMAIFRSLSLYMADAGEFRSQNQLY
                     GSIGSASVLGVPVPVWILLALAAVLSLALRRTRYGRYVRAVGSNQRVARYSAIDVDRV
                     RFVAYLLNGLAVGVSATLLSMRFNSVGSSTLGQSYELDAIAAVIIGGTAMEGGRGTVW
                     GTIVGAVILGVINNMLNMVGVSPYLQGTVKGLVIIGAVFIQRRRQE"
     gene            complement(3581..5089)
                     /locus_tag="PLG14_09000"
     CDS             complement(3581..5089)
                     /locus_tag="PLG14_09000"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOX49307.1"
                     /translation="MEKSFALLENIEKSFSTVSVLKKVTVGVGQGEILGLIGENGAGK
                     STLMKILSGIHEPNAGRILVEGKERRFRRPADAKAAGIALVPQEFNLCPDLSVAENVF
                     LGAEPLAKGGLLDQAAMRTRSAALLAELGARIDPDARISRLSAAQKQIVEICKALAFD
                     ARLLILDEPTTVLTPVEIHALFALMRALKAKGMSMVYISHKLGEVKEICDRVAILRDG
                     ELVHEAPAAGLSPAEMASRMVGRELSEIYPEPAEVRGEVLLKVEGLSSPGAFEGVSLE
                     LRSGEVLGLAGLVGAGRTEVAEAIMGLRPASGRVEVGGEELRLGRPREAIAAGVGYLS
                     EDRQGSGVIVGFSVNENETLASLPSYARGLFGPIDRRRERAAAEKWARLFSIKAASAD
                     ERLEHLSGGNQQKVSLAKSLDPAPRVLIVDEPTRGVDVGAKREIYRLLRDLAAEGIAI
                     LLISSELEEVLGMCGRVLVMREGRAAGELSGAGLTEEGIMFLATGIKEGVSA"
     gene            complement(5299..5619)
                     /locus_tag="PLG14_09005"
     CDS             complement(5299..5619)
                     /locus_tag="PLG14_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49308.1"
                     /translation="MAARRKSLPTGALAGILGALLLVALNPTTADFKAFYQRQAASGV
                     SSKAGGSGGLIGSIAKGAAGIAADSSYKRANLLVASLYQSRSRDGEVQRAYLGIAKTF
                     IKIK"
     gene            complement(5629..>5861)
                     /locus_tag="PLG14_09010"
     CDS             complement(5629..>5861)
                     /locus_tag="PLG14_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969321.1"
                     /note="with UgpABC is involved in uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HOX49309.1"
                     /translation="IQFIYGWNQYLWPLLITTKEKYYTLLIGINRMLAGADVLIEWQI
                     VMATTLLAMIPPVLVVVVMQKQFVAGMTDTEK"
BASE COUNT          887 a         1906 c         2153 g          915 t
ORIGIN      
        1 cgcctcgtag gagggatagg cgcgctcgat gccgagctcg cgggccttgc gcgcggccgt
       61 ctcggcgtcg cgctcgacca gtcccgcgac ggggacgccg aggcgccgca gcgcctcgag
      121 gtgcgccggc ccgatgaagc cggtgcccac gacgagggcc cggggcctgc cgctctggtt
      181 catggctatg ctccttgggg ccgcgctagg gcgcgatgtc gcgcatgagg cggcccacgt
      241 tgttctgcga gaatacctcg tccaggacga ggaccgcggc cccgaccacg ccagcccgct
      301 cgcggagggc ggagcgcttg acgacgaggt cggaggtggc gagcggagtc gagcggcggt
      361 agatggactc gcggatggag gcgaggtagc gctcgcccag gtcggagagc ccgccgccga
      421 cgacgatgag cgaggggttg aagaagttga cgagcttggc gaggacgttg cccagggcct
      481 tgcccgctgc gatcacgacg cggaggcact cctcgtcgcc gtgcgccgcg cccagggcca
      541 ggagctcggg gctcggcgcc tcgcccgcgg cgagctgggc cgccaggaag gggctcgcgc
      601 ccgacctcgc ggcctcgtcg gcgagcaggg cgagggcctt gccgccggcc acggcctcca
      661 ggcagccgtg gtttccgcag cggcagaggg tctcgacgcc gtcgatgccg atgtggccta
      721 tgtctccggc cgcgcccttg gcgccgcggt agatcttgcc gtccacgacg atgcccgcgc
      781 cgatgcccga gcccgccttc acgagcatga agtctcgctc gcccgcggcg gcccccttct
      841 cgcgctcggc cagggccatg acgttcacgt cgttgtcgac gaagacgggg caggtgaact
      901 cgcgggcgag ctgggaggcg acggggaagc ggtcccatcc cggcatcacg gggggcgaga
      961 ccggcacgcc gtgctggtag tcgacggggc cggggaggcc gacgccgacg ccgaagagcg
     1021 aggagggcga gaggccgttc ttcgcggcca ggctccgcgc cgcctccgcg atgcgggaga
     1081 ggacggcctc cgggccctcg cgcgagaagt cgacgcggcc ggcggagagg tccaggacat
     1141 cggcctcgag gtcgcagagg gccacgttga ggtgggtctg ccccaggtcc acgccgacca
     1201 cgtagcccgc ggactcggcg accttgaggg tgcggcgctt gccgcccgac ttggtctcct
     1261 cgcgcacgag gccgacggag aggagcttct cgacgtagag ggagacggtg gacttcgcga
     1321 agccgctccg cctcgcgatg tcggcggggg agagggggcc gtggtccctg atgatcctga
     1381 ttatcctgcc ggcggaggtc gagggtccgg ccccggctcc ttcctctcgt tccttcacgc
     1441 gtcgtcctcg catcgaaata gggttaggca gtcttccccg cgcgggcccg gggcgtcaag
     1501 cgccgtcggg ccagcgcggg cggccgcgcc cggccgtccg cccgcgggaa gggctcccgc
     1561 ggacggcggc cgggaagggg cttagaacac ggactcgggg acgtagtagc tcttggcgtt
     1621 ctcgcgggtg atgatctcgg agttgaggat gatgcggatc gggagcttct tctggtagaa
     1681 gccctcgaag acgcggccgc ggaggccgac gacggccatg gagaccgcgg tggcgcagac
     1741 gtcgggcgga taggtggcgt tggcgcggac gagggggtcg ccgtccatga tgcgcttgat
     1801 catcgacttg gaggcggcgc cgccgaacat gatcttcacg tccttgcggc ctgactcctt
     1861 gtaggcctgg agcacgccct cgagcatgtc gtcgtcggcg gtgtagacgg cgtcgatctg
     1921 cttgtacttc tggaggtagt tctccatgac ggccagggcc ttctccttgt tccagtagcc
     1981 gggctgggag tcgaggacct tgatcccggg gtacttggcg gcgacgtcct tggccacctt
     2041 ggtgcggagg tcggagatca cgctcgggac gccctcgatg aggacgaggt tgcccttgta
     2101 gccgagctcc ttgcagatcc actccatcga gttgcgggcg taggcctcgt cgtcgttgga
     2161 gaggtagacg tcgtagacgg gcttcgtcac gccgcggtcc aggaccacga cgtagatgcc
     2221 cttcgcgtag gccttctcga cgacgggggt gagggcggcg gactcgtgcg ggaacacgac
     2281 gaggccgttg acgccctgga cgatgaggtc ctcgatgtcg gcgacctgct tggcgacgtc
     2341 gggggcggtg cggaagagga aggtcacgtt cgggtccttc ttcttccagt cgtccatggc
     2401 gcgcttggcc caccagttgg cgccgcccat ccagccgtgg tcggccgtcg gcatggtcac
     2461 gccgatggtg atcttcttcg tggcctgggc ctggccgaag gccagccccg tgacggcgac
     2521 gaggacgagg agagtcgcga ggatcttctt cattcgatgc ctcctgtcgg aaaccgaaaa
     2581 cttctaggcc cccgatgggg gccgaaccgc ccggcctatt cctgcctccg gcgctggatg
     2641 aagaccgcgc cgatgatgac gagtcccttg acggtcccct gcaggtaggg ggacacgccg
     2701 accatgttca gcatgttgtt gatgacgccc aggatgacgg cgccgactat cgtgccccag
     2761 accgtgccgc gtccgccctc catcgccgtg ccgccgatga tgacggcggc tatcgcgtcc
     2821 agctcgtagc tctgccccag ggtggaggag ccgacggagt tgaagcgcat ggacaggagg
     2881 gtggcggaga cgccgaccgc gaggccgttc aggagatagg cgacgaagcg cacgcggtcc
     2941 acgtcgatcg ccgagtagcg cgcgacgcgc tggttggagc cgacggcccg cacgtagcgg
     3001 ccgtagcgcg tccgcctcag ggccagggag aggacggccg ccagggccag gaggatccac
     3061 accggcacgg gcacgcccag gacagaggcc gagccgatgc tcccgtagag ctggttctgc
     3121 gagcggaact cgcccgcgtc ggccatgtag agggagaggg agcggaagat cgccatcgtg
     3181 cccagggtca cgatgaaggg ggcgatgcgt cctttcgtga cgaggaggcc gttggccgcc
     3241 ccgccggccg cgccgacgaa gagcgcgacc gcgacggcga cggcgacgcc cgagggctcc
     3301 gagaggccct tcgccgcggc ggcgttcatc gcgagcaccg cgagggagcc cgtgaaggcc
     3361 gcgagggagc cgacggagag gtcgatgccg cccgcggtga tgacgaaggt catgccgagg
     3421 ccgatgatgc cggtgtagga gacctgcctc aggatgttga ggaggttctg cggctgcagg
     3481 aaatgggggc tcagggccgc cgaggccgcc gcgagcacgg cgagggccac gaggctcccg
     3541 aagcgctcga agtcgagccg cggcgccttc cgtatcgtcg tcatgcgctc actccttcct
     3601 tgattccggt ggcgaggaac atgatgccct cctccgtgag ccctgcgccc gagagctctc
     3661 ccgccgcacg gccctcccgc atcacgagga cgcggccgca catgcccagc acctcctcga
     3721 gctccgagga gatgaggagg atcgcgatgc cctccgccgc gaggtcgcgg aggaggcggt
     3781 agatctcccg cttcgcgccg acgtcgacgc ccctcgtggg ctcgtcgacg atgaggacgc
     3841 ggggagccgg gtccaggctc ttcgcgaggg agaccttctg ctggttgccg cccgagaggt
     3901 gctcgagccg ctcgtcggcg gaggcggcct tgatcgagaa gagcctggcc cacttctccg
     3961 ccgcggcgcg ctcgcggcgc cggtcgatcg ggccgaagag gcccctcgcg taggagggga
     4021 gggaggccag cgtctcgttc tcgttgacgg agaagccgac gatgacgccc gagccctggc
     4081 ggtcctcgga gaggtagccg acgcccgccg ctatcgcctc gcggggccgc cccaggcgca
     4141 gctcctcgcc gcccacctcg acgcggcccg aggccgggcg gagccccatg atcgcctccg
     4201 ccacctcggt cctgcccgcc ccgaccaggc ccgcgagccc cagtacctcg cccgagcgca
     4261 gctcgaggga gacgccttcg aaggcccccg gcgaggagag cccctcgacc ttgaggagga
     4321 cctcgccgcg gacctcggcc ggctcggggt agatctccga gagctcgcgg cccaccatcc
     4381 ggctcgccat ctccgcgggc gaaaggccag cggcgggagc ctcgtgcacg agctcgccgt
     4441 cgcggaggat cgcgacgcgg tcgcagatct ccttgacctc gccgagcttg tgcgagatgt
     4501 agaccatgga catgcccttg gccttgaggg cgcgcatcag ggcgaagagg gcgtggatct
     4561 ccaccggcgt gaggacggtc gtcggctcgt ccaggatgag gaggcgggcg tcgaaggcca
     4621 gggccttgca gatctcgacg atctgcttct gggcggcgga gagcctcgag atgcgcgcgt
     4681 ccgggtcgat cctggcgccg agctccgcga ggagggcggc cgacctggtc cgcatcgccg
     4741 cctggtcgag gagtccgccc ttggccaggg gctcggcgcc gaggaagacg ttctccgcga
     4801 cggataagtc tgggcagagg ttgaactcct ggggcaccag cgcgatgccc gcggccttgg
     4861 cgtcggccgg gcgccggaag cggcgctcct tcccctcgac gaggatccgg cccgcattcg
     4921 gctcgtggat gccggagagg atcttcatga gggtggactt gccggcgccg ttctcgccga
     4981 tgaggcccag gatctcgccc tggcctacgc ctacggtcac cttcttgagg acggagaccg
     5041 tggaaaagct cttctcgatg ttctcgagca gagcgaagga tttctccatg atcgctcccg
     5101 cggggttcta gggtgtcgac gcgtctcgcg cgccgggcgt cgatctcgtt gttttagtat
     5161 tgaatgactt tgtccggctg tcaatctctg aaagatcgaa aaagaactag aaagttcgaa
     5221 aacgaactag cgaagcgggc ggagcgacaa agaaaagggc gcccctcggg gcgccctcgg
     5281 ctcgcggaga ccggaagcct acttgatctt gatgaaagtc ttcgcgatcc cgagataggc
     5341 gcgctgcacc tccccgtccc tcgacctgct ctggtacagg ctggcgacga ggaggttcgc
     5401 gcgcttgtag ctggaatccg cggcgatgcc cgccgcgccc ttcgcgatgc tgcctatgag
     5461 gccgcccgag ccgcccgcct tgctggatac gcccgacgcc gcctggcgct ggtagaaggc
     5521 cttgaagtcg gccgttgtcg gattcagggc cacgaggagg agggccccga ggatgccggc
     5581 cagggcgccc gtgggaaggc ttttccgtct tgctgccatg ctgtctcctt atttctccgt
     5641 gtcggtcatg ccggcgacga actgcttctg catcacgacc acgaccagga cgggcgggat
     5701 catcgcgagg agggtcgtcg ccatgacgat ctgccactcg atgaggacgt ccgcgccggc
     5761 gagcatgcgg ttgatgccga tgaggagggt gtagtacttc tccttcgtcg tgatgaggag
     5821 gggccagagg tactggttcc agccgtagat gaactggatg a
//
LOCUS       DAOOSJ010000175         5840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8509_length_5840_cov_5.333449, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000175 DAOOSJ010000000
VERSION     DAOOSJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8509_length_5840_cov_5.333449"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..855)
                     /locus_tag="PLG14_09015"
                     /pseudo
     CDS             complement(106..855)
                     /locus_tag="PLG14_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
     gene            1455..1679
                     /locus_tag="PLG14_09020"
     CDS             1455..1679
                     /locus_tag="PLG14_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOX49310.1"
                     /translation="MLKEKVLRAIQDVRPSLQADGGDIELVDVGEDGIVKVRLTGACG
                     SCPMAVMTLKQGVESYLKKVIPEVKEVVQA"
     assembly_gap    1855..1954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1955..2973)
                     /locus_tag="PLG14_09025"
     CDS             complement(<1955..2973)
                     /locus_tag="PLG14_09025"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOX49311.1"
                     /translation="MRNLVSKIVEATPGLRVADKAMNGVFALQKIPRCDPDVIVLDIE
                     MPEMNGIEFLKERRRLGLEIPVVILSSVAKEGAAVTMEALSLGAVDFITKPSGSVSAD
                     IHTVAAELSRLLLAFGAQYQRKRGKGAPTYEFEAPERLFEPAPVLEAKAKAKVAPPKP
                     LRQPGPLELIAIGISTGGPNALREVFAKIDADLPQPVVVVQHMPPGFTEEFAKSLDRI
                     CPLDVKEAADGDLLKPGRVLIAPGDRHVEVERRPLGSVVRLSEAPPVNGHRPSADVLF
                     ASAAKAYGNRAMGVIMTGMGRDGARELGSIFREGGVTLGQDEASSVVYGMPKAAFELG
                     HVTEQV"
     gene            complement(3029..3844)
                     /locus_tag="PLG14_09030"
     CDS             complement(3029..3844)
                     /locus_tag="PLG14_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOX49312.1"
                     /translation="MSDFLSDADFELYRKLIYDESGIHFSATNRSILESRLKERLREK
                     KSDSLRDYYKLILGDKEEFKTLLDSVTTNLTRFFRNQPHFDALEHYVVPELAKLRGAS
                     ADRKLRIWSAGCSTGEEPYTIAMLLKEILPAGFSFEVVASDISLKSLMVAKEGFYGDS
                     RIQGIPESYLAKYFERKPSGYQLKDEVKKLVRFDYHNLKNDSGLRNLDVVFCRNVLIY
                     FDEAAQKATIERFWESMAPKSFLFIGHSESLFGMNTRFEFVKTDWACFYRKFT"
     gene            4054..4596
                     /locus_tag="PLG14_09035"
     CDS             4054..4596
                     /locus_tag="PLG14_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOX49313.1"
                     /translation="MKQDFVEVRVHGVSVGPGEGAPMVLLLDGRGEAGLPVPVGPFEA
                     SAIIIELEGISPPRPLTHDLLAELFREGGLSLERAELYGELGSGGWRGLPDSLGLAFP
                     EGGFRAAGAPLARLVYRRGLRRREREVRPSDAIALALRLGAPICAERGLLGSRAEAEA
                     AMRSYAPGPELHRQSAGEAL"
     gene            4669..>5840
                     /locus_tag="PLG14_09040"
     CDS             4669..>5840
                     /locus_tag="PLG14_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49314.1"
                     /translation="MGLFSSIKAFFVRIFTRDPDEARRRAELKRIHAVLTEMRPSYYK
                     PKQNLVLTGFAQALYSFCLALKPLSELVRSTVAASDSRVSQRYFDYLIECRLPKATQD
                     RKANFGYDGMAARLASSLDPERELELVASEFQGLLSEVESLSVRDLNIELGEMDRFIE
                     ICRHDFERLLGLFDPGIAVDSPSYRPDFSPAEGEQAFPEIVDLYYLFQGFFVSAELKA
                     NLARLLERRSSSGLEEAKRKKLDKIAARLDELLSGRLDPELLLALARATKGDPTLLPA
                     TARERRDFVESYRRRLAQQFEKDRERLQRERHESVVAADIKSLFADGDIMEVDGYDDE
                     TDAYLRRESPNGFSYVKPLRILKTFVLAAFDSSIKEPVKRLLVEGYFDNKGFQNNLA"
BASE COUNT          847 a         1911 c         2077 g          905 t
ORIGIN      
        1 ctccgcgtcg ccgacggcgg cgaagaggcc gggctcggcg ctctcgggct gccgggcgga
       61 ggaggcgacg gggccggcga tggctcccgc ctccgtcccc tcgacccggg cgatgagctc
      121 gcgggccagc gactcgtagc tcttggcccc gatgcaggag gggtcgtaga ggcagacggg
      181 caggccgtgg gagggcgcct ccgagaggcg gacgttccgc ggcacgatgg tgcggaagac
      241 gcggtccttg aagtactcgg agacctgctg gaccacttcc tgggccagct tggtcctgga
      301 gtcgtacatc gtgaagacga tgccccccac ctggaggcgg gggttcatgc tcttctggac
      361 gaggctgacg gtctggagga tgagggagag gccctccagg gcgaagtact cgcactggag
      421 ggggatgagg acctcgtcgg cggccacgag gccgttcagg gtcaggacgc ccagggaggg
      481 ggggcagtcg atgaggacgt agtcgtagtc cgcggagacg gtggcgaggg cgcgcttcag
      541 gtagccgttg cggtcttcct tgtcgacgag ctcgacggtc gcgccggaga ggtcgatgga
      601 ggagggcacg agggagagcc gctcgacctt ggtcgcctgg acgacctcgg ccatcgtcgc
      661 ctggccgctc accacctcgt acacgccctt ggcgccgtgg acgccgccga cgccgctcgt
      721 gcagttcgcc tggggatcga agtcgacgag aagggtccgc ttgcccttga gggcgagata
      781 ggctccgagg ttgatcgcgg tcgtggtctt gcccacgccg cctttctggt tgacgaatac
      841 gattgtgcgt gccatcggac ctctccccag gaagttcagg catgatagca caggattccg
      901 cgctctacca agcccgcccg cgacgggccg aggcggcggc cggggcttcg cacccttcga
      961 ccggtctcgg gcggccgggc tggacgggtc ctggggggcg ggaaggcgcg gggccctcgc
     1021 tgaaggctcg gggcccgcgt cttcccgccc cccacccgtc gtcccgccag gtctcccggg
     1081 cgcttcgagg ggtttgacgc ctcgggccgc gtctcgggca ggcgcttcgc ctcgagggtg
     1141 gggagaagag gtcggcgcga ggcggctgga cggcgagcgg cggctcggga cgccgcgacc
     1201 gtacggggag cggcggctgg ccaaggccag cgccgacctg accgtggggt gccgcttggc
     1261 acggggcgac cggtcggcga gcggcggctc aggacgccgc gaccgcacgg ggagcggcgg
     1321 ctggctgaag ccagcgccga ccttaccgtg gggcgcggct tggcacggtg cgaccgttcg
     1381 gggagcgacg gcgacatggg cggcttgacc ggagcggggg acggtagata aattgtagag
     1441 aaaggagagc atgcatgctc aaagagaagg tactgcgcgc catccaggat gtgcgtccct
     1501 cgctccaggc ggatggcggg gatatagagc tcgtggacgt gggagaggac ggaatcgtga
     1561 aggtgcggct gaccggcgcc tgcggttcct gccccatggc cgtgatgact ctcaagcagg
     1621 gggtggagag ttacctcaag aaggtcatcc ccgaggtcaa ggaagtcgtc caggcctgaa
     1681 gcgcctcgag cccggcttcc cggagaagtg cggccgcctc tcggggcggc cgtatttttg
     1741 gggtgcgccg gtcctcagcg gacgccgtcg tcgaaggggc cgtcgtcgtc ctcgtagcgg
     1801 tcctgctcgg cggtcatggg gtggggaggc gggggcgcca tgagccgcgc gcccnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacctgc tcggtgacgt ggccgagctc
     1981 gaaggcggcc ttgggcatgc cgtagacgac gctggaggcc tcgtcctggc ccagggtgac
     2041 gccgccctcc cggaagatgg agccgagctc ccgggcgccg tcgcggccca tgccggtcat
     2101 gatgacgccc atggcccggt tgccgtaggc cttcgcggcg ctcgcgaaga ggacgtcggc
     2161 gctggggcgg tggccgttga cgggcggggc ctcggagagg cggacgacgg agccaagggg
     2221 acggcgctcc acctcgacgt ggcggtcgcc gggggcgatg agaacgcgcc ccggcttgag
     2281 gaggtcgccg tcggccgcct ccttcacgtc cagggggcag atccggtcca ggctcttcgc
     2341 gaactcctcc gtgaagccgg ggggcatgtg ctggacgacg acgacgggct ggggcaggtc
     2401 ggcgtcgatc ttcgcgaaga cctcgcgcag ggcgttgggg ccgccggtgg agatgccgat
     2461 cgcgatgagc tcgagggggc cgggctggcg caggggcttg ggcggcgcga ccttggcctt
     2521 cgccttggcc tccaggacgg gcgcgggctc gaagaggcgc tcgggcgcct cgaactcgta
     2581 ggtgggcgcg cccttgcccc gcttccgctg gtactgggcg ccgaaggcga ggaggaggcg
     2641 cgagagctcg gcggcgacgg tgtggatgtc ggcggagacg gagcccgagg gcttcgtgat
     2701 gaagtcgacg gcgcccaggg agagggcctc catcgtgacg gcggcgccct ccttcgcgac
     2761 ggaggagagg atgacgaccg ggatctcgag gccgaggcgc ctgcgctcct tcaggaactc
     2821 gatgccgttc atctccggca tctcgatgtc caatacgatg acgtcggggt cgcagcgcgg
     2881 gatcttctgg agggcgaaga cgccgttcat cgccttgtcg gcgaccctga ggccgggggt
     2941 ggcctcgacg atcttcgaga ccaggttgcg catcagcgcc gagtcgtcca cgacgagtac
     3001 ggagatcggg tccggcacgg gattccccct aggtgaattt gcggtagaag caggcccagt
     3061 cggtcttcac gaactcgaac ctcgtgttca tcccgaagag cgactcggag tggccgatga
     3121 agaggaagga tttcggggcc atcgactccc agaagcgctc gatggtcgcc ttctgggccg
     3181 cctcgtcgaa gtagatgagg acgttgcggc agaagaccac gtcgaggttc ctgagtcccg
     3241 agtcgttctt gaggttgtgg tagtcgaagc ggacgagctt cttcacctcg tccttgagct
     3301 ggtagcccga gggcttcctc tcgaagtact tcgcgaggta ggactcgggg atgccctgga
     3361 tgcgggagtc gccgtagaag ccctccttgg cgaccatgag ggacttgagg ctgatgtcgc
     3421 tcgccaccac ctcaaaggag aagcccgcgg gaaggatctc cttgaggagc atcgcgatcg
     3481 tgtagggctc ctcgcccgtg gagcagccgg cgctccagat gcggagcttg cggtcggccg
     3541 aggcgccgcg gagcttcgcg agctcgggca cgacgtagtg ctccagggcg tcgaaatggg
     3601 gctggttgcg gaagaagcgc gtcaggttcg tcgtgacgga gtcgaggagg gtcttgaact
     3661 cctccttgtc gccgagtatg agcttgtagt agtcgcggag gctgtcggac ttcttctcgc
     3721 ggaggcgttc cttgagacgg ctttcgagga tggaacggtt ggtcgccgag aagtgtatgc
     3781 cgctctcgtc gtagatgagc ttccggtaga gctcgaagtc ggcgtcgctg agaaaatcgg
     3841 acatgcccgt ggcgcctttc cccgcgaggc gccgtcttac gccccgcgga tacagccaaa
     3901 agtctaggcg ccgggcatgg aggtgtcaat accgcgcctt gcgcgccctt gcgcgccctt
     3961 gcgcgtcccc gcgcgccgga tcggccgcgt tccgcgccgg tttggcggcc ctccgcgcgg
     4021 aaagggcggg aaaggcgggc tatattggac ggcatgaagc aggatttcgt ggaagtgcgg
     4081 gtccacggcg tctccgtggg ccccggcgag ggcgccccca tggtcctcct cctggacggc
     4141 aggggggaag cgggccttcc cgtgccggtc ggccccttcg aggcgagcgc catcatcatc
     4201 gaactcgagg gcatctcgcc gccccgcccc ctcacccacg acctcctcgc cgagctcttc
     4261 cgcgagggcg gcctctccct ggagcgggcc gagctctacg gcgagctcgg ctcgggcggc
     4321 tggcggggcc tcccggactc cctgggcctg gccttccccg agggcggctt ccgcgcggcc
     4381 ggcgcccccc tcgcgcggct cgtctaccgc cggggccttc ggcgccggga gcgggaggtc
     4441 aggccctccg acgcgatcgc cctggccctg cggctcggcg cgcccatctg cgcggagcgg
     4501 ggcctcctgg gctcccgggc ggaggcggag gccgcgatgc ggtcctacgc ccccggcccc
     4561 gagctccacc gccagagcgc cggcgaggcc ctctagggaa ggcccgaaaa tcgggcatcg
     4621 cagggattgc gccttgccgg cgcggccgcg gggccctaca atggctccat gggcctcttc
     4681 tcatcgataa aagcgttctt cgtccgcatc ttcacccgcg accccgacga ggcgcgcagg
     4741 cgcgccgagc tcaagcgcat ccacgcggtc ctcacggaga tgcgcccctc ctactacaag
     4801 cccaagcaga acctcgtcct cacgggcttc gcccaggccc tctactcctt ctgcctggcc
     4861 ctgaagcccc tctccgagct cgtgcgctcg acggtcgccg cctcggattc ccgcgtctca
     4921 cagcgctact tcgactacct catcgagtgc cgcctcccca aggccaccca ggaccggaag
     4981 gccaacttcg gctacgacgg catggccgcg cgcctggcct cctccctcga ccccgagcgc
     5041 gagctcgagc tcgtggcctc cgaattccag ggcctcctct cggaggtcga gtccctctcc
     5101 gtccgagacc tcaacatcga gctgggcgag atggaccgct tcatcgagat ctgccgccac
     5161 gacttcgagc gcctcctcgg cctcttcgac cccggcatcg ccgtcgactc cccgagctac
     5221 cggcccgact tctcgccggc cgagggcgag caggccttcc ccgagatcgt cgacctctac
     5281 tacctcttcc agggcttctt cgtctccgcc gagctcaagg ccaacctcgc gaggctcctg
     5341 gagcggcgct cctcctcggg actggaggag gccaagcgca agaagctcga caagatcgcc
     5401 gcccgcctgg acgagctcct ctcgggccgc ctggaccccg agctcctcct ggccctggcc
     5461 cgcgcgacca agggcgaccc caccctcctg cccgccaccg cgcgggagcg gcgcgacttc
     5521 gtcgaatcct accggcgccg cctggcccag cagttcgaga aggaccgcga gcggctccag
     5581 cgcgagcgcc acgagagcgt cgtcgccgcg gacatcaaga gcctcttcgc ggacggcgac
     5641 atcatggagg tggacggcta cgacgacgag acggacgcct acctccgccg ggagagcccg
     5701 aacggcttct cctacgtgaa gcccctgcgc atcctcaaga ccttcgtcct cgcggccttc
     5761 gactcctcca tcaaggagcc cgtgaagcgc ctcctcgtcg agggctactt cgacaacaag
     5821 ggcttccaga acaacctggc
//
LOCUS       DAOOSJ010000176         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8553_length_5824_cov_5.401456, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000176 DAOOSJ010000000
VERSION     DAOOSJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_5824_cov_5.401456"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLG14_09045"
     CDS             <1..846
                     /locus_tag="PLG14_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49315.1"
                     /translation="AELSDERFLELFREAIDLGVGTLMVAGGEPLVRRSLMEGIARLP
                     GVVVPVFTNGSLVDERYLELFSSGRLVPVFSVEGDSAFTAERRGAGIHETVLARCAEL
                     KERGALFGFSVTLTSRNADAVLSEGFLRGLEGLGASVLFLIEYVPVTPGSEGLVLSDA
                     QKAELSREGRFDGYSFMTISLPGDEEAFGGCLAAGRGFIHLADDGALEACPFAPFSDT
                     SAADRSLREALDSPLMRAIRARHAELTETKGGCALWNQKGWVASLGACGRSSGEAALR
                     EAEPA"
     gene            1039..1560
                     /locus_tag="PLG14_09050"
     CDS             1039..1560
                     /locus_tag="PLG14_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX49316.1"
                     /translation="MPRPDVSRPDASLPRRPLSRLVLGLAPVFILAGGAFFPALGLVV
                     PLILLAAVLGGLKRRRWFCSAACPRASLLEILGSRLTRRRPLPPALRSPALRTALCGL
                     LMLCAVGQAARLWPSLQAMGAFFWLVCLVTLALSLLMAFLWKPRAWCAVCPVGALQDN
                     IAARTAPRPSQAE"
     gene            1570..2928
                     /locus_tag="PLG14_09055"
     CDS             1570..2928
                     /locus_tag="PLG14_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX49317.1"
                     /translation="MNSLRVRKTAHGLVRWGILAGVLAYVSWEFYAHAAVSKVHPSVH
                     ALCPLGGLESLLRYVAGDGATLGKIFSGTMGLFFVSLGTALLFKRAFCGNVCPLGTLQ
                     ELAGNLGRKVLGPRRFLVPRRLDKVLRWAKYLALALAVVMAWATGTLWIQAVDPWPAY
                     SHLFSPSELFPAYAAGLVLLAASLVASFFYERAFCKYLCPMGALTAVAGLASPFKARR
                     DEAACVSCGLCDQACPVNLEVSKAAAVADAECLSCGKCAAVCPAPKALDLGFSKRPRL
                     SPAAAVALGAGAFFLGVLALQLLGFDRFSGRQEPTLRELARSQGQTAAEFKEAYGLPS
                     GLYGGTRASGLEKALPLSKFAEMNGTDAAALKATLGLDPALDEDVSWGEAYGSVKLGR
                     IAELNGMSLEDFKKTFSLKASAGADTPWREVEKAVIRASERLAAEAAAAEGEGGGSCS
                     GD"
     gene            complement(2980..4602)
                     /locus_tag="PLG14_09060"
     CDS             complement(2980..4602)
                     /locus_tag="PLG14_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOX49318.1"
                     /translation="MRIGKGLVALFCLCILLAPRVAWAAEGGKRPPDLRGLLAPGERA
                     WLSAHPVIRVAGDPVWGPIEYRDREGKFVGMTPDYLAWIGEGLGVEFRYGEEASWAEC
                     IESLRTGRADMAATLMATPERRSWLLMSRPYISFRYAVFGLPGSPYIERLADLAGSRV
                     LVVAGFGVEELLRRDFPGLELVEKSTAEEAVAALARGGADFFINDIATTSWYVEKLGL
                     TNVKATGDSPYVMELVMGVRKDLAALASILDKALAAMPEAEARAIRREWFSAQPAKPA
                     NYDYLFAFAAAAAVVVTAALAWNRILAGRVRERTADLAFANECLIAEIAERKKAEAGL
                     SSALEERGRLLSELNHRVKNNLQLALSLVRLSSSAAEGEGREALDGALGRLEAIARVH
                     ETLDPEEALVDLKVDLGLYLENLAADERTLAAEGGLVSLETELERGVRVSMKTAANVG
                     LVVNELLTNARKYAFPEGRRGRISLRARALPEGGAAVEVADDGVGMDPGAAPRVDSLG
                     MSIVRSIVEGFGTSLELKTAPGEGTSWRFVVP"
     gene            4783..5535
                     /locus_tag="PLG14_09065"
     CDS             4783..5535
                     /locus_tag="PLG14_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX49319.1"
                     /translation="MNEILRSLFERKSARSFLDRPIGGAEKDLILDSAIQAPTAGNQA
                     LYTILDIEDQALKEALALSCDDQPFIAKAPLVLVFLADCRKWLDFYRYAGVEAREPGP
                     GDLLLACADALVAAQNSVVAAESLGIGSCYIGDILENREKVVELLGLDDYTMPAAMLV
                     YGYPAESALARRKPPRFGRKYLVRKDRYARLSEEEAREMLASTHPEPGFDFDAYMEAF
                     AKRKYLSAFALELDRSAGEYLRRFAERDRPRS"
     gene            complement(5599..>5824)
                     /locus_tag="PLG14_09070"
     CDS             complement(5599..>5824)
                     /locus_tag="PLG14_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49320.1"
                     /translation="YRSISLASTANLDDLSALSFLSYETEPFQGLAVLLRAALPLDRA
                     ALGIGDGEGELGPDALGYRVSFSARAKLRF"
BASE COUNT          794 a         2143 c         1955 g          932 t
ORIGIN      
        1 gccgagctct ccgacgagcg cttcctcgag ctcttccgcg aggccatcga cctgggcgtg
       61 ggcacgctga tggtcgcggg cggcgagccc ctcgtccggc gctccctcat ggagggcatc
      121 gcccgcctgc cgggcgtcgt cgtgcccgtc ttcacgaacg gctccctcgt ggacgaaagg
      181 tacctcgagc tcttctcctc cggacgcctc gtgccggtct tcagcgtcga gggcgactcg
      241 gccttcaccg ccgagcgccg cggcgcgggc atccacgaga ccgtcctcgc gcgctgcgcc
      301 gagctcaagg agcgcggcgc cctcttcggc ttctccgtga ccctcacctc gcggaacgcc
      361 gacgccgtcc tctccgaggg cttcctccgc ggcctcgagg gcctgggcgc ctccgtcctc
      421 ttcctcatcg aatacgtccc ggtcacgccc ggcagcgaag gcctcgtcct gagcgacgct
      481 cagaaggccg agctctcccg cgaggggcgc ttcgacggct attccttcat gaccatctcc
      541 ctccccggcg acgaggaggc cttcggcggc tgcctcgcgg ccggccgggg cttcatccac
      601 ctggccgacg acggagccct ggaggcctgc cccttcgcgc ccttctccga cacgagcgcc
      661 gcggaccgct ccctcaggga ggccctcgat tcgcccctca tgagggccat ccgagcgcgc
      721 cacgccgagc tcacggagac caagggcggc tgcgccctct ggaaccagaa gggctgggtg
      781 gcctccttgg gcgcctgcgg caggagctcg ggcgaagccg cgcttcggga ggcggagccg
      841 gcctaggcgg gtccggccgc tttggccgat ggccggcgca gcttagtggc ttcgccggcc
      901 cggaggcttt gccagtctgg aggctttgcc gcggggccgg cggctctcaa atttctgtag
      961 gctggcggtc ctgcatgctg gcaatataga ataattgcta tattgccagc atgcatagta
     1021 ctatgctatc gtcgcccaat gccgcgccct gatgtcagcc gccctgatgc cagccttcca
     1081 aggcgacccc tctcacgcct cgtcctgggc cttgcccccg ttttcatcct cgcgggaggt
     1141 gccttcttcc ccgccctggg cctcgtcgtg cccctcatcc tcctcgcggc cgtcctgggc
     1201 ggcctcaaga ggcggcgatg gttctgctcc gccgcctgcc ccagggccag tctcctcgag
     1261 atcctgggct cacggctgac gcgcaggcgg cccctgccgc cggcgctccg ctcgcccgcc
     1321 ctgcgcacgg ccctctgcgg cttgctcatg ctctgcgccg tcggccaggc ggcccggctc
     1381 tggccctcgc tccaggcgat gggcgccttc ttctggctcg tctgccttgt gaccctcgcc
     1441 ctgtccctcc tcatggcctt cctctggaag cccagggcct ggtgcgcggt ctgccccgtc
     1501 ggagccctcc aggacaatat agccgcccgg accgccccgc ggccgagcca agcagaataa
     1561 ggagaaacga tgaactcgct tcgcgtccgc aagaccgccc acggactagt ccgctggggc
     1621 atcctcgccg gggtcctggc ctacgtttcc tgggagttct acgcccacgc ggcggtgagc
     1681 aaggtacacc cctccgtcca cgccctctgc cccttgggag gcctcgaatc cctcctccgc
     1741 tacgtcgcgg gcgacggggc gaccctcggg aagatcttct ccggcacgat gggcctcttc
     1801 ttcgtctccc tcggaacggc cctcctcttc aagcgcgcct tctgcgggaa cgtatgcccc
     1861 ctgggaaccc tccaggagct ggccgggaac ctgggccgca aggtcctggg accgcgccgc
     1921 ttcctggtcc cgcggcgcct cgacaaggtc ctgcgctggg cgaagtacct ggccctcgcc
     1981 ctggccgtcg tcatggcctg ggccacggga accctctgga tccaggccgt cgatccctgg
     2041 cccgcctact cccacctctt ctcgccctcc gagctttttc cggcctacgc ggccggcctc
     2101 gtcctcctcg cggcctccct cgtcgcctcg ttcttctacg agcgcgcctt ctgcaagtat
     2161 ctctgcccca tgggcgcgct cacggccgtc gccggcctcg cctcgccctt caaggccagg
     2221 cgcgacgagg cggcctgcgt ctcctgcggc ctctgcgacc aggcctgccc ggtcaacctg
     2281 gaggtctcca aggccgccgc ggtggccgac gccgagtgcc tctcctgcgg gaagtgcgcg
     2341 gccgtctgcc cggcccccaa ggccctcgac ctgggcttct cgaagcggcc tcggctgagt
     2401 ccggccgcgg ccgtcgccct gggcgccggc gccttcttcc tcggcgtcct ggccctgcag
     2461 ctcctcggct tcgaccgctt ctcaggccgg caggagccga ccctccgcga gctcgccagg
     2521 agccagggcc agaccgccgc cgagttcaag gaggcctacg gactcccctc ggggctctac
     2581 ggaggcacca gggcgagcgg gctcgagaag gcccttccgc tttcgaagtt cgcggagatg
     2641 aacggcacgg acgccgccgc gctcaaggcg accctcggcc tcgatcctgc cctggacgag
     2701 gacgtgagct ggggcgaggc ctacggcagc gtcaagctag ggcggatcgc cgagctaaac
     2761 gggatgagcc tcgaggattt caagaagacc ttctcgctca aggcctccgc cggggccgac
     2821 acgccctggc gggaggtcga gaaggcggtc atccgcgcct cggagcgttt ggcggccgag
     2881 gccgcggcgg cggagggcga gggcgggggc tcctgctccg gggattgagg agggcaagga
     2941 gtcagtacgc cgcggcggac gggatcgagc gggccggggc tagggtacga cgaagcgcca
     3001 ggaggtgccc tctccgggcg ccgtcttgag ctcgaggctg gtgccgaaac cctcgacgat
     3061 ggagcggacg atgctcatgc ccagggagtc gaccctgggt gcggctcccg gatccatgcc
     3121 caccccgtcg tcggccacct cgaccgcggc gccgccttcc ggcagggccc tggccctgag
     3181 gctgatcctg ccccggcggc cttcggggaa ggcgtacttc cgcgcgttgg tcaacagctc
     3241 gttcacgacc aggcccacgt tggccgccgt cttcatggag acgcgcacgc cgcgctccag
     3301 ctccgtctcg agcgagacga ggccgccctc cgcggcgagg gtccgctcgt ccgcggcgag
     3361 gttttcgagg tagagcccca ggtccacctt gaggtcgacc agggcctcct cggggtccag
     3421 ggtctcgtgg acgcgggcga tggcctcgag ccgccccagg gccccgtcca gggcttcgcg
     3481 gccctcgccc tccgctgccg aggaggagag ccgcaccagg gacagggcga gctggaggtt
     3541 gttcttgacg cggtggttga gctcggagag aaggcggccc ctctcctcca gggccgacga
     3601 gagtcccgcc tccgccttct tgcgctcggc gatctccgcg atgaggcatt cgttggcgaa
     3661 ggccaggtcc gccgtcctct cacgcacccg ccccgccagg atgcggttcc aggccagcgc
     3721 ggccgtgacg acgacggccg cggccgccgc gaaggcgaag aggtagtcgt agttcgctgg
     3781 cttggcgggc tgggcggaga accattccct gcggatcgcc ctggcctccg cctcgggcat
     3841 cgcggccagg gccttgtcca ggatcgaggc gagggcggcg aggtccttgc ggacgcccat
     3901 gacgagctcc attacatagg ggctgtcgcc cgtcgccttc acgttggtca ggccgagctt
     3961 ctcgacgtac cagctcgtgg tcgcgatgtc gttgatgaag aagtccgccc cgccccgcgc
     4021 gagggccgcg accgcctcct ccgcggtgga cttttctacg agctcaaggc ccgggaagtc
     4081 gcgccgcagg agctcctcga cgccgaagcc agccacgacc aggacgcggg agcctgcgag
     4141 gtcggcgagc ctttcgatgt agggcgagcc gggcaggccg aagacggcgt agcggaaact
     4201 gatatagggc cggctcatga ggagccaact ccgccgctcg ggcgtggcca tgagggtggc
     4261 ggccatgtcc gcgcgcccgg tccttaggga ttcgatgcac tcggcccagc tcgcctcctc
     4321 gccgtagcgg aattcgacgc ccaggccctc gcctatccag gctaggtagt cgggggtcat
     4381 gccgacgaac ttgccctccc tgtcccggta ctcgatgggt ccccatacag gatcccccgc
     4441 gacccggatg acgggatggg cggagagcca ggccctctcc ccgggggcga gtaggccgcg
     4501 aaggtcgggc gggcgcttcc ccccctcggc ggcccaggct acgcggggcg cgaggaggat
     4561 gcagaggcag aacagggcga ccaagccctt gccgatgcgc ataccataag ggtaggtccc
     4621 gctttccgcg ctgtccagtg acaaagcggg ctaattgcgt cgccaaagag cctcaaggcc
     4681 cggtgattcg ggtcgacagg gacggcctca tggtgtagaa cgatcctcgc gccgcgcggc
     4741 cgcttccttc catccttggg cgcgcgcggc ccggaggaag gcatgaacga gatcctgagg
     4801 tcgctcttcg agaggaagtc ggcgagatcc ttcctcgatc ggcccatagg cggggccgag
     4861 aaggacctca tcctggactc ggccatccag gcgccgacgg ccggcaacca ggccctctac
     4921 accatcctcg acatagagga ccaggccctc aaggaggccc tggccctctc ctgcgacgat
     4981 cagcccttca tcgccaaggc gcccctcgtc ctcgtcttcc tggcggactg ccggaagtgg
     5041 ctcgacttct atcgctacgc gggcgtcgag gcgagggagc ccgggccggg ggacctcctc
     5101 ctcgcctgcg ccgacgccct ggtcgccgcc cagaactccg tcgtggcggc cgagtccctc
     5161 gggatcggct cctgctacat cggcgacatc ctggagaacc gggagaaggt cgtggagctc
     5221 ctcggcctcg acgactacac catgcccgcc gcgatgctcg tctacggcta tcccgcggaa
     5281 tcggcgctcg cgcgccgcaa gcctccccgc ttcggccgga agtacctggt caggaaggac
     5341 cgctacgcgc gcctctcgga ggaggaggcc cgggagatgc tcgcctcgac ccaccccgag
     5401 cccggcttcg acttcgacgc atacatggag gccttcgcga agcgcaagta cctctcggcc
     5461 ttcgccctgg agctggaccg cagcgcgggc gagtacctaa ggcgcttcgc ggagcgggac
     5521 cggccgcgga gctgaggccc gcctggtgcg gccttgcctg ccctgcgtcg cgcgcggcta
     5581 tcccgcttgc gcggcctcct agaaccgcag cttcgcccgg gccgagaagg agactcggta
     5641 tcccagggcg tcgggcccga gctcgccctc tccgtccccg atcccgaggg cggcgcggtc
     5701 caggggaagg gccgcgcgga gaaggacggc aagaccctgg aagggctccg tctcgtagga
     5761 gaggaaggaa agggcggaga ggtcgtcgag gttcgcggtg gaagcgaggc taatgctccg
     5821 gtag
//
LOCUS       DAOOSJ010000177         5765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8668_length_5765_cov_6.180035, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000177 DAOOSJ010000000
VERSION     DAOOSJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8668_length_5765_cov_6.180035"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PLG14_09075"
     CDS             complement(<1..1664)
                     /locus_tag="PLG14_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOX49321.1"
                     /translation="MRRNSFGRAAKPLAILLLALAAAAGFVACATASAAYTPGTYVGV
                     GKGHGGELKVAVTVSERAILKIEVLSAGETAVLGDSAFEELTRRVIAANSAEVDTVSG
                     ASDSSEGFLAAVRGALAAAGFKGGASGAKTTARAPAKAYDVIVVGGGGAGLSAANAAA
                     AAGAKVVVFEKMGVLGGNTLRATGGLNAAATKYQAAKGVADSPELFFKDTMKGGYNKN
                     DPALVKLLSEKAVDSLDWIESMGGDLSDVGRAAGASVDRIHRPAGGAKAGPEIVKTLK
                     GAAEAKSGISFFTDARVTALQADKKGAVSGVVVAIGGKDYKVAAKAVVLASGGFGANE
                     EMLVALNPALKGFATTNHPGATGDGIKLAEAVGAALVDMKEIQTHPTYAPGKEMVTEA
                     VRGNGAILVDHKGARFIDELKTRDVVSAAILGLPEKTAYLVFDTSVRKSLKAIEDYVK
                     MGIVVEAATPAELAAKIGVDPAVLASTIERYNGFVAAKADADFARPDMPRALSVGPFH
                     AIEVTPAVHHTMGGVKIDTSARAISAKGQPIPGLFAAGEVTGGVHGG"
     gene            1808..>2521
                     /locus_tag="PLG14_09080"
     CDS             1808..>2521
                     /locus_tag="PLG14_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX49322.1"
                     /translation="MQDLARLDLPIAPYLPEIERLLLERGSLVLSASPGAGKTSLAPL
                     FLASGGRLGGKVLCLEPRRIACVQAAARAAELLGEEVGGQVGYRVRLESRTSPRARLE
                     FVTEGILVRMIQDDPGLEGIALLVFDEFHERSVQADLGLALALESRALRPDLRLLLMS
                     ATLDTERLASFLSAPLLEVPGRAYPVVTRYEALAGADRASGGTGGWGAQAGGAGAGRG
                     GLESGIASLARRLAGEAEGD"
     assembly_gap    2522..2621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2622..4349
                     /locus_tag="PLG14_09085"
     CDS             <2622..4349
                     /locus_tag="PLG14_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HOX49323.1"
                     /translation="VTEREARDRAEQRRGRAGRLGPGLCLRAWAASESLPERTEAEIL
                     RAELSSLVLEAALWGARSPGDLPWIDEPPAASWEAARELLAELGALDSGGRITEFGRA
                     AAGLGAEPRLAALVLRGAEAGRLREAAALAALLGERLPAEDGEGDLGERLARLARGEP
                     AYARIREVAAELARQAERLAPRGHLPGPSAARQSGAAGYGAAGYGALLARAFPDRVAR
                     RSERRVSARGPEASFRIPGGRLLRARGALAEADWIVAAEVDAGGAGGSLAARLGAADE
                     GTVFSGAALSEDEALEALASLVEDRVELEWEGLHCRARRRRRAGSILLSETPLGPPSP
                     EAAGQALAARVAAEGLGILPWEAGGDASGAGSAGAFLARARWLFGAAEAEAAPPGAGT
                     PGSAPAAAPPPGAAAPGAAEAALRLSPEWMARNAAEWLAPFVHGGPGPVLDGAALRRA
                     AEALLPWEARAALDRLAPERLGLPSGSSRPVLYAEGADPVVEARVQEFFGLSEQPRIA
                     GRPVVARLLTPAGRPLQVTADLPGFWRGSWAEARKELRGRYPKHDWPEDPAAAAPSAR
                     GFKKKPPAP"
     gene            4450..5565
                     /locus_tag="PLG14_09090"
     CDS             4450..5565
                     /locus_tag="PLG14_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX49324.1"
                     /translation="MDKTFGSLAEGGIDLLESSFTALDAYLGLKPAPLRFALASGSLV
                     ELAKAAEGLEYPGLSYADAALSEPGEEGEEERRLYIRLADSPAAAEPSAFAPLDLLRD
                     PESGVYHDPKGVYGELRSPLLEPRPAPAETAAFEAAVLLARYGYELPEDFLPEPPRDF
                     PPAAQRDLLELVLTGRTPERAFGFLARTGFVEAYWPELAELRGVGHSKEYHPEGDAWE
                     HTMETFRYRKLPDLRLSLALLLHDTGKPGASSSGGRRFDRHAEIGRAVAARFLARLGF
                     GRRLSDDVAYLVRYHMLPAALPRLPLGRGIEGLDDPRFPLLLELYKCDELSTFRGPDG
                     YYESCAAYRAYLRNSRNPYRGPEGRKLARTFLEGSAT"
BASE COUNT          652 a         2176 c         2067 g          770 t
ORIGIN      
        1 ccgccgtgga cgccgcccgt cacctcgccc gcggcgaaga ggccggggat gggctggccc
       61 ttcgccgaga tcgcgcgcgc ggaggtgtcg atcttgacgc cgcccatcgt gtggtggacg
      121 gccggcgtga cctcgatcgc gtggaagggg ccgacggaga gggcgcgggg catgtcgggc
      181 cgcgcgaagt ccgcgtccgc cttcgcggcg acgaagccgt tgtagcgctc gatcgtcgaa
      241 gccaggaccg cggggtcgac gccgatcttc gccgcgagct ccgcgggggt cgcggcctcg
      301 accacgatgc ccatcttcac gtagtcctcg atggccttga ggctcttccg caccgaggtg
      361 tcgaagacga gataggcggt cttctcgggg aggccgagga tggccgcgga gacgacgtcg
      421 cgggtcttga gctcgtcgat gaagcgcgcg cccttgtggt cgaccaggat ggcgccgttg
      481 ccgcgcaccg cctcggtgac catctccttg cccggcgcgt aggtcgggtg ggtctggatc
      541 tccttcatgt cgacgagggc cgcgccgacg gcctcggcga gcttgatgcc gtcgcccgtg
      601 gctccggggt ggttcgtcgt cgcgaagccc ttgagggcgg ggttgagggc gacgagcatc
      661 tcctcgttcg cgccgaagcc gccggaggcg aggacgaccg ccttcgcggc caccttgtag
      721 tccttgccgc cgatcgcgac gacgacgccg gagacggcgc cctttttgtc cgcctgaagg
      781 gcggtgacgc gggcatcggt gaagaaggag atgccgctct tggcctcggc cgcgcccttc
      841 agggtcttca cgatctcggg gccggccttc gcgccgcccg caggacggtg gatacggtcg
      901 acggaggcgc ctgccgcgcg gccgacgtcg gagagatcgc cgcccatgga ctcgatccag
      961 tcgagcgagt cgacggcctt ctcggagagg agcttcacga gggcgggatc gttcttgtta
     1021 tagccgccct tcatcgtgtc cttgaagaag agctcggggc tgtcggcgac gcccttcgcg
     1081 gcctggtact tcgtggccgc ggcgttgagg ccgccggtgg cgcgcagggt gtttccgccg
     1141 aggacaccca tcttctcgaa gacgacgacc ttcgcgccgg cggccgcggc ggcgttcgcg
     1201 gccgaaaggc cggcgccgcc gccgcccacc acgatcacgt cgtaggcctt ggcgggagcc
     1261 cgcgcggtgg tcttggcgcc ggaggcgccg cccttgaagc cggcggcggc gagggcgccg
     1321 cggaccgcgg ccaggaagcc ctcgctcgag tcggaggcgc cggagaccgt gtccacctcg
     1381 gcggagttgg ccgcgatcac gcggcgggtg agctcctcga aggccgagtc gccgaggacg
     1441 gcggtctcgc ccgcggaaag gacctcgatc ttgaggatgg cccgctcgct cacggtgacg
     1501 gcgaccttga gctcgccgcc gtggcccttg ccgacgccga cgtaggtgcc gggcgtgtag
     1561 gcggcggagg ccgtcgcgca ggcgacgaag cccgcggcgg cggccagggc gagaaggagg
     1621 atggcgaggg gcttggctgc cctcccgaag ctgtttcttc gcatagcgac tccttggagg
     1681 ctgatgagcc accatagaga gtttcgagat attgcgtcaa gtagtttcga taatttcata
     1741 tcgctacaat tttcgcatca ccctccctcg ccttgcccaa ggggccggct tcgggcacac
     1801 tgcccccatg caggacctcg cccggctcga cctccccatc gccccctacc ttccggagat
     1861 cgagcgcctc ctcctcgagc gcggaagcct cgtgctctcc gcgagcccgg gcgcgggcaa
     1921 gacgagcctc gcgcccctct tcctcgcctc aggcgggcgg ctgggcggga aggtcctctg
     1981 cctcgagccc cgcaggatcg cctgcgtcca ggccgccgcc cgcgcggccg agctcctggg
     2041 cgaggaggtc ggcggccagg tcggctaccg ggtgcggctc gagtcgagga cgagcccccg
     2101 cgcccgcctc gagttcgtga cggagggcat cctggtccgc atgatccagg acgatcccgg
     2161 ccttgagggc atcgccctcc tcgtctttga cgagttccac gagcgctcgg tccaggccga
     2221 cctgggcctg gccctggccc tggagtcgcg ggccttgcgg cccgacctca ggctcctcct
     2281 catgtccgcc accctggaca cggaaaggct cgcctccttc ctctccgcgc ccctcctcga
     2341 ggtgccgggc cgggcctatc ccgtggtgac acgctacgaa gccctggccg gcgcggaccg
     2401 ggcctcggga gggacaggcg gctggggcgc gcaggcgggc ggagcggggg ccggacgcgg
     2461 cggccttgag tcgggcatcg cgtccctggc caggcgcctc gcgggcgagg ccgaggggga
     2521 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcaccgag cgcgaggccc
     2641 gcgaccgcgc cgagcagcgc cggggccgcg ccggccgcct ggggccgggc ctctgcctcc
     2701 gcgcctgggc cgcctcggag agccttcccg agcggacgga ggcggagatc ctccgcgccg
     2761 agctctcctc cctcgtcctc gaggccgcgc tctggggggc gcggagcccc ggcgacctgc
     2821 cctggatcga cgagccgccg gccgcctcct gggaggccgc gagggagctc ctggccgagc
     2881 tcggcgccct ggactcgggc gggcggatca cggagttcgg ccgcgcggcc gccggcctcg
     2941 gcgctgagcc caggctcgcc gccctggtcc tgcgcggcgc cgaggccggg cgcctccgcg
     3001 aggcggcggc cctcgccgcc ctcctcggcg agcggctccc cgcggaggac ggcgagggcg
     3061 acctggggga gcgcctcgcg cggctcgccc ggggcgagcc cgcctacgcc cgaatccgcg
     3121 aagtggcggc cgagctcgcc cgccaggccg agcggctcgc gccccgaggc cacctgcccg
     3181 gaccgtcagc cgcgcgccag tccggcgccg ccggctacgg cgccgccggc tacggagccc
     3241 tcctcgcgag ggccttcccc gaccgcgtcg cgaggcgctc ggagcggcgc gtctccgccc
     3301 gcggccccga ggcgagcttc cgcattcccg gcggccgcct cctccgcgcg cggggcgccc
     3361 tcgccgaggc cgactggatc gtcgccgcgg aggtcgacgc gggaggggcg ggaggctctt
     3421 tggcggcccg gctcggcgcg gccgacgagg gcaccgtctt ctcgggcgcc gccctgtccg
     3481 aggacgaggc cctcgaggcc ctcgcctccc tcgtggagga cagggtcgag ctcgagtggg
     3541 agggcctcca ctgccgggcg cgccgccgcc gccgcgcggg gagcatcctc ctctcggaga
     3601 cgcccctcgg cccgccctcc cccgaggcgg cgggccaggc cctggccgcg cgcgtcgccg
     3661 ccgagggcct gggcattctc ccctgggagg cgggcgggga cgcctcgggc gcgggctccg
     3721 cgggcgcctt cctcgcgcga gcgcgctggc tcttcggcgc cgccgaggcc gaggcggcgc
     3781 cccccggcgc ggggacgccc ggctcggccc cggcggccgc cccgcccccc ggagccgcgg
     3841 cccccggcgc ggccgaggcc gccctgcggc tctcccccga atggatggcg cgcaacgcgg
     3901 cggagtggct cgcgcccttc gtccatggag ggcccgggcc cgtcctggac ggcgcggccc
     3961 tgcggcgcgc ggccgaggcc ctcctcccct gggaggcccg cgccgccctg gacaggctcg
     4021 cgcccgagcg cctcggactc ccctcgggct cctcccgtcc cgtcctctac gcggaagggg
     4081 ccgacccggt cgtcgaggct cgggtccagg aattcttcgg cctctccgag cagccgcgga
     4141 tcgcgggccg gcccgtcgtc gcgaggctcc tcacgccggc cggccgcccc ctccaggtga
     4201 cggccgacct ccccggtttc tggaggggct cctgggccga ggccaggaag gagctccgcg
     4261 gccgctaccc caagcacgac tggcccgagg atccggccgc cgcagcgccc agcgcgcggg
     4321 gcttcaagaa gaagccgccc gctccctgaa ggccggccgc gctcggcctt cagcccccag
     4381 ggcccggtcg gcgcggggcc cgcgaaggca tggtttacag ccgcggccgg aaatcgctat
     4441 cttcttccca tggacaagac cttcggcagc ctcgccgaag ggggcatcga cctcctcgaa
     4501 tcctccttca ccgcgctcga tgcctacctg gggctgaagc ccgcgcccct gcgcttcgcc
     4561 ctggcctcgg gctccctggt ggaactcgcg aaggcggccg agggcctcga gtaccccggc
     4621 ctctcctacg ccgacgccgc cctttccgag ccgggcgagg aaggggagga ggagcgacgc
     4681 ctctacatcc ggctggccga ttccccggcg gcggccgagc cctcggcctt cgcccccctc
     4741 gacctcctcc gcgatcccga gagcggcgtc taccacgacc ccaagggcgt ctacggcgag
     4801 ctcaggtccc cgctcctcga gccgcggccc gcgccggcgg agaccgccgc cttcgaggcc
     4861 gccgtcctcc tggcccgcta cggctacgag ctccccgagg acttcctccc ggagccgccc
     4921 cgcgacttcc ccccggccgc ccagcgcgac ctcctcgagc tcgtcctcac ggggcggacg
     4981 ccggagcggg ccttcgggtt cctggccagg acgggcttcg tcgaggccta ttggcccgag
     5041 ctggccgagc tcaggggcgt gggccactcc aaggagtacc atcccgaggg cgacgcctgg
     5101 gagcacacga tggagacctt ccgctaccgg aagctccccg acctccggct ctccctggcc
     5161 ctcctcctcc acgacacggg caagcccggg gcgagctcct cgggcgggag gcgcttcgac
     5221 cgccacgcgg agatcgggcg cgccgtcgcg gcgcgcttcc tcgcccgcct cggcttcggg
     5281 cggcggctct ccgacgacgt cgcctacctg gtgcgctacc acatgctgcc ggcggccctg
     5341 ccgcgcctgc ccctgggccg cggcatcgag ggcctcgacg acccgcgctt ccccctcctc
     5401 ctcgagctct acaagtgcga cgagctctcc accttccgcg gccccgacgg ctactacgag
     5461 tcctgcgccg cctatcgcgc ctacctccgg aactccagga atccctaccg cggcccggag
     5521 ggcaggaagc tcgccaggac cttcctggaa gggagcgcga cttgagcgcg cggacggccg
     5581 ggcctagact ttttgggaga gcctcgggga gggcgcgcga tggcgaatga ggggcgcgag
     5641 ggcgccttgg cggccgcggc cgggctggcg acggccctct tcggccccgg cgggaccggc
     5701 aggccgagcg agctcctcgc cgcgaaggcc ttcatgggct tcctcgagcc cgcgggcgtg
     5761 gggat
//
LOCUS       DAOOSJ010000178         5745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8715_length_5745_cov_5.836380, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000178 DAOOSJ010000000
VERSION     DAOOSJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8715_length_5745_cov_5.836380"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PLG14_09095"
     CDS             <1..1496
                     /locus_tag="PLG14_09095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOX49325.1"
                     /translation="ERATLESRIAQVGEAEVLTRPELAEAAGWARVFSGKAAETDAFL
                     ERLEAAAAGLPEGKARDDLSGAAAAMRAFASVQRGLLREAEAAARRAESLLGPDRHFS
                     RNIVPFVLGSCARMRGDLRQAEGIMREFLRRSREWGDLYDMMMAYYEVCLTARQLGRL
                     GEASEAYREAMAELARRGARGFGSACKVHGNYGEILYERGELDALDALLEGHLDGGGD
                     WNLPTDILVAMSPAVKSRIARGRLEEARGLLLRAEGIEAKSGTFPRIAVLYRDLRARL
                     SIAEGSPAAPPPAAYPPELVAVAQAARATDLRVLLASGSPAEAAAGASALAAEALSGG
                     GLALFVEAKALEAAALAEAGEEGKALAALGEALAPARGEGFVRSLVDAGEGMRRLLAL
                     GLEEGRAERLGPAEAAYARRLLASFPPNGEASPGAARATAPEGLASARELEVLALLAE
                     GLGNKEIASRLFISEGTVKTHVHHLAEKLEAKSRGAIVVRARALGLL"
     gene            1679..1894
                     /locus_tag="PLG14_09100"
     CDS             1679..1894
                     /locus_tag="PLG14_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49326.1"
                     /translation="MESDKKDYEIRIRGAIEPGWAEWLDAAEARAEGGDSLLFLRAAD
                     RAALYGALRILGDLDCRLVSVRCLEPQ"
     gene            1944..2828
                     /locus_tag="PLG14_09105"
     CDS             1944..2828
                     /locus_tag="PLG14_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49327.1"
                     /translation="MKKTTRTGMAALCLAIALLAGAASAAGVFLRGDGETGQTTSLRG
                     ESYRYAANGVYKWNAERVVAEGVGWDIFTLFLAVPALAAAAFLVAKGSFRGRLFAVGM
                     LGYFFYQYLEYATYWAYGPLFLLYVAIYGASLGGMAWILSSLGLKDMASRFSENFPRK
                     GMAGLCFGMSFALVFVMWLPLVLRSMGGDIEGVLLGQTTLVVQAFDLGLIVPLAVFTG
                     VAILRRRPVGYLLSAVFVVKAVAMAGAITAMLLSAWKVEGRLEVPPFLFFGGATLFAL
                     WLLVRMYASFAPEASPAE"
     gene            3036..3482
                     /locus_tag="PLG14_09110"
     CDS             3036..3482
                     /locus_tag="PLG14_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOX49328.1"
                     /translation="MKDRMAIPGKGRAALLLASTLLAAAALLATSCSGVALSGTGDFA
                     KGLPATMPDLSAKAAGDYQGSYTIALPAGAIAMSRRWEVTVTVDASHAMSAIAVDYPA
                     SYPDEGMIDTLKARVLAAQSLDVDAFSGATYSSKAFLKAVEDALSD"
     gene            3466..4158
                     /locus_tag="PLG14_09115"
     CDS             3466..4158
                     /locus_tag="PLG14_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49329.1"
                     /translation="MPSLTKARKVLLAGLLTAIFVAAFAAAPAAAEEAAPAGLAEPLP
                     AAASSSGAAAGSAAAVGPARISAAYLDAGALGSGIAYSAFHLRGGAAFPLAGRDTWGF
                     EAEFYWTRPDDLAFFQADALGLYRRRLGVGGFFLGAGAGLGFSRAAFAGSSGGAEAYA
                     ETRIKAILLAEAGYALDLFGTRLYLEPFVRAFAAAGPDARSGAALAPGEAQEGWSASA
                     SGEVGLRLGWRF"
     gene            4335..4733
                     /locus_tag="PLG14_09120"
     CDS             4335..4733
                     /locus_tag="PLG14_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOX49330.1"
                     /translation="MRIDHVAIWTNDLERLKEFYRDRLGGQPGEKYQNHAKGLETYFL
                     RFGDGARLEIMRRPEVGLREGAGDYPRGYAHLALSVDNPQAVDAAVARLKAAGVVVQG
                     EPRWTGDGYYEAVVLDPDGNRIELVAERRA"
     gene            complement(4840..5241)
                     /locus_tag="PLG14_09125"
     CDS             complement(4840..5241)
                     /locus_tag="PLG14_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX49331.1"
                     /translation="MAADVLGIGKRILLFMGLAYLIQALITRYLPEGFVSGLLGKESP
                     FAIPLATLVGVPFYTTGISALGLAAGFLGKGMSEPAVLAFLVGGAVTTLPAMSAVYGI
                     VRPRVFGLYLGFCAAGALLAGYAMQLAKALL"
BASE COUNT          782 a         2124 c         2056 g          783 t
ORIGIN      
        1 gcgagcgggc gaccctggag tcgcgcatcg cgcaggtcgg cgaggcggag gtcctcacgc
       61 ggcccgagct cgcggaggcg gcgggctggg cccgcgtctt ctcggggaag gccgcggaga
      121 ccgacgcctt cctggaacgg ctcgaggccg ccgcggcggg cctgccggag ggcaaggcga
      181 gggacgacct ctcgggggcg gcggccgcga tgcgagcctt cgcctccgtc cagcggggcc
      241 tcctccgcga ggccgaggcg gccgcgagga gggccgagtc cctcctgggg ccggaccgcc
      301 acttctcccg caacatcgtc cccttcgtcc tcgggagctg cgcgaggatg cgcggcgacc
      361 tgcgccaggc ggaggggata atgcgggagt tcctgcgccg gtcgcgcgag tggggcgacc
      421 tctacgacat gatgatggcc tactacgagg tctgcctcac ggcgcgccag ctggggcggc
      481 tcggggaggc ttccgaggcc taccgcgagg cgatggccga gctcgcccgg cgcggcgccc
      541 gcggcttcgg atcggcctgc aaggtgcacg gcaactacgg agagatcctc tacgagcgcg
      601 gggagctcga cgccctggac gccctcctgg agggccacct ggacgggggc ggggactgga
      661 acctcccgac ggacatcctc gtggccatgt cccccgcggt gaagtcgcgc atcgcgcggg
      721 gtcgtctcga ggaggcccgc gggctccttc tccgggcgga ggggatagag gcgaagtcgg
      781 gcaccttccc ccgcatcgcc gtcctctacc gggaccttag ggcgcggctt tcgatcgcgg
      841 agggctcgcc cgccgctcct ccccccgcgg cctaccctcc cgagctcgtc gcggtcgcgc
      901 aggccgcacg cgcgaccgac ctccgcgtcc tcctcgcctc gggctccccg gccgaggcgg
      961 ccgctggcgc ctcggccctg gccgcggagg cactctcggg cggcggcctc gccctcttcg
     1021 tcgaagccaa ggccctggag gccgcggccc tggccgaggc gggggaggaa gggaaggccc
     1081 tcgccgccct cggcgaggcc ctggctcccg cccgcgggga gggctttgtc cgcagcctcg
     1141 tcgacgcggg ggagggcatg aggcgcctcc ttgccctcgg cctcgaagag gggagggcgg
     1201 agaggctcgg cccagccgaa gccgcctacg cccgccgcct cctcgcctcc ttccccccga
     1261 acggggaagc gagcccgggg gcggcgcgcg ccaccgcgcc cgagggcctc gcgagcgccc
     1321 gcgagctcga ggtccttgcc ctcctcgccg aggggctcgg caacaaggag atagcctctc
     1381 gcctcttcat ctcggagggg acggtcaaga cccacgttca ccacctggcc gagaagctcg
     1441 aggccaagag ccgcggcgcc atcgtcgtcc gcgcccgcgc cctcggcctc ctctagcccc
     1501 gggacgaggc tccacccgag gctccacccg aggccggccc cccgccgctc ccagctgccc
     1561 gcctaacaac tgcacctcgg ccgtcctcac gagccggcca cgcgtcggat cgccctaaaa
     1621 tccatctttc gatggatagc gggccccggc ggtttaggcc tatcttccag gcaacgacat
     1681 ggaaagcgac aagaaggact acgagatcag gatacggggc gccatcgagc cgggctgggc
     1741 cgagtggctc gacgcggccg aggcgagggc ggaagggggc gacagcctcc tcttcctcag
     1801 ggccgccgac cgcgccgccc tctacggggc gctccggatc ctcggggatc tcgactgcag
     1861 gctggtctcg gttcgttgcc tcgaaccgca atgataggga aagcgcggag ggccccctgg
     1921 ggcgccgcgg gaggagactt gaggtgaaga aaaccacgag gacggggatg gcggcgctct
     1981 gcctggccat cgccctgctg gccggcgccg cgagcgcggc cggcgtcttc ctccggggcg
     2041 acggagagac cgggcagaca acgagccttc gcggcgagag ctaccgctac gccgcgaacg
     2101 gcgtctacaa atggaacgcc gagcgggtcg tcgcggaggg cgtgggctgg gacatcttca
     2161 ccctcttcct cgccgtcccc gccctggccg ccgcggcctt cctggtcgcg aagggctcct
     2221 tcaggggtcg gctcttcgcg gtcgggatgc tcggctactt tttctaccag tacctcgagt
     2281 acgcgaccta ctgggcctac gggccgctct tcctcctcta tgtcgccatc tacggggcga
     2341 gcctgggagg catggcctgg atcctttcga gcctggggct caaggacatg gcctcccgct
     2401 tctccgaaaa cttcccgcgg aagggcatgg ccggcctctg cttcggcatg tccttcgccc
     2461 tggtattcgt gatgtggctg cccctcgtcc tgcgtagcat gggcggcgac atcgagggcg
     2521 tcctcctcgg ccagacgacc ctcgtcgtcc aggccttcga cctcggcctc atcgtgcccc
     2581 tggccgtctt cacgggggtc gccatccttc ggcgccggcc agtcggctac ctcctctccg
     2641 ccgtcttcgt cgtgaaggcg gttgcgatgg ccggcgccat caccgcgatg ctcctctccg
     2701 cctggaaggt cgagggaagg ctcgaggtcc cgcccttcct cttcttcggg ggggcgacgc
     2761 tcttcgcgct ctggctcctc gtcaggatgt acgcgagttt cgcgcctgag gcctcgccgg
     2821 cggagtagcg ggagctcccg cagggagagg gccgccgggc cgcgaacccg gcggccacgc
     2881 gccgaaccgc tctccacccc gctctcctct ccgcacggca gcgcgccggg ccgccctaag
     2941 ctccgcgcgc cggccggcgg cctgagcctt ggcgagatag ccgatccgcg gcggccgccc
     3001 ggccgagcgc tgcgagactc ttacaaagga acgctatgaa agacagaatg gccattccgg
     3061 ggaagggacg ggccgcgctc ctcctcgcat ctaccttgct cgccgcggcc gccctcctcg
     3121 ccacgtcctg ctccggcgtc gcgctctcgg gcacgggcga cttcgcgaaa ggcctcccgg
     3181 cgacgatgcc cgacctttcg gcgaaggccg cgggggacta ccagggctcc tacacgatag
     3241 ccctgccagc aggggccatc gccatgtccc ggcgctggga ggtgaccgtg acggtcgacg
     3301 catcccacgc tatgtcggcg atcgccgtcg attatcccgc gagctatccc gacgagggca
     3361 tgatcgacac gctcaaggcg cgggtgctcg ccgcgcagtc cctcgacgtg gacgccttct
     3421 cgggcgccac ctattcctcc aaggctttcc tcaaggcggt cgaagatgcc ctctctgact
     3481 aaggcgcgca aggtcctcct cgccggcctg ctaaccgcca ttttcgttgc agccttcgcc
     3541 gccgctcccg cggccgcgga ggaggcggcc cccgcagggc tcgccgagcc gctcccagcc
     3601 gcggcctcca gctccggcgc cgcagccggc tcagccgcgg ctgtcgggcc agcccggatc
     3661 tcggcagcct acctcgacgc gggagccctc ggctcgggaa tcgcctactc ggccttccat
     3721 ctccgcgggg gagccgcctt tcccctcgcg ggccgcgaca cctggggctt cgaagcggaa
     3781 ttctactgga cgcggccgga cgacctcgcc tttttccagg ccgacgccct cggcctctac
     3841 cgtcgccggc tcggcgtagg gggcttcttc ctgggcgccg gcgccggcct cggcttctcc
     3901 cgcgccgcct tcgccggctc gtctggcggg gcggaggcct acgcggagac gcggatcaag
     3961 gccatccttc tcgcggaggc gggttatgcg ctcgacctct tcgggacgag gctctacctc
     4021 gagcctttcg tccgcgcctt cgcggccgcc ggcccggacg cgcgctcggg cgcggccctg
     4081 gctccaggcg aagcccagga gggctggtcg gcctcggcct ccggcgaggt cggcctcagg
     4141 ctgggctggc gcttctgagt ggcccggcca cgtgagtccg gcgcgttccg gcgccgatgg
     4201 ctaaagcccc cgcgccgagc cgcgggggct tctctttgcc cgtcgagccg aggccgccgc
     4261 acgcaatcgg cgtagcgcgc cgcggttccg cgcggagcgg actcgcgcta tactcgaggc
     4321 accggaggac cagcttgcgc atcgaccatg tggcgatttg gacgaacgac ctcgagcgcc
     4381 tgaaggaatt ctaccgcgac cgcctgggcg gccagcccgg agagaagtac cagaaccacg
     4441 cgaagggcct ggagacctat ttcctccgct tcggcgacgg agccaggctc gagatcatgc
     4501 gccggcccga ggtggggctc cgcgaaggcg cgggggacta tcctcggggc tacgcccacc
     4561 tggcgctctc cgtggacaat ccgcaagcgg tcgacgcggc cgtcgcgcgg ctcaaggccg
     4621 ccggcgtcgt ggtccagggg gagccccgct ggacgggcga cggctactac gaggccgtgg
     4681 tcctcgaccc ggacggcaac aggatcgagc tcgtcgccga gcggagggcc taggagcctg
     4741 ggctggagcg gcgccgcggg ggcgccgccc gcgccgcacc caacgccagc gccgcaggcc
     4801 accaacgcgc ggcaggccgc gaacccgccg cttcgtcggc tagagcaacg ctttcgcgag
     4861 ctgcatcgca tagcccgcga ggagggctcc cgccgcgcag aagccgaggt agaggccgaa
     4921 gacccgcgga cggacgatgc cgtacaccgc ggacatggcc ggcaaggtcg tcaccgcgcc
     4981 gccgaccagg aaggccagca ccgccggctc gctcatgccc ttgccgagga agcccgcggc
     5041 caggccgagc gcggagatcc ccgtcgtgta gaaggggacc ccgaccaggg tggcgagggg
     5101 tatcgcgaag ggactctcct tcccgaggag gccggagacg aagccttcgg gcaggtagcg
     5161 ggtgatgagc gcctggatga ggtaggcgag gcccatgaag aggaggatcc gcttgccgat
     5221 cccgaggacg tcggcggcca tgccggcgaa tcccccctcg cggcccttgg actggcagcc
     5281 gcaggaagaa gcggcggacc cgcccgaggc ggaggcttgg gcgcttagcg cctcgattgc
     5341 ccggccgcag ccgcaggccg gctccggggc ggcggccgcc gcgccgcagc cgcaggcccg
     5401 ctcccaggga gcggcggctg ccgcgtccga gtgaagcggc cgcgaccggg cggaactcca
     5461 tggcggcccg cggcgcagga gccgaagcgc cgcagcaggc gacggcgggc cgggcaccga
     5521 gggccgcgag ccgggcaccc aggggggcga agagcctgag cggcgacgcc gcggcccgcc
     5581 gttcctttcc ggccttggcc gagccgcgga ggatgtcggc gcccaggaag ccgctccgct
     5641 ccagggccag agtcgcgtag ccggcgccca ggctcatcgc gaaggtggcc gcgaggcgcc
     5701 agaccgcgag ctcccagccg agggagccga cggagaggaa gaaga
//
LOCUS       DAOOSJ010000179         5727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8741_length_5727_cov_7.020628, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000179 DAOOSJ010000000
VERSION     DAOOSJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8741_length_5727_cov_7.020628"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLG14_09130"
     CDS             complement(<1..567)
                     /locus_tag="PLG14_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49332.1"
                     /translation="MRIETYFARPELDGRGGSLASRLGRVLPSPLSVRIVDVVLTDFP
                     VPRDAAAELFGDPVVQVLAEGPAPLDPGLGPWDWLVEIAYRPGVTDTLALTAREALAI
                     GLGPETVEGRVVQTSRLYALALPGSPGERAVLKGEVERAFAPLWNSLVQSAVFLSRAE
                     WASGARLPELYPEVRLSDPAEPERIDVAS"
     gene            complement(677..2494)
                     /locus_tag="PLG14_09135"
     CDS             complement(677..2494)
                     /locus_tag="PLG14_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49333.1"
                     /translation="MNSGFYKRAIGTTLLYIGIFVLLVLIQFSGAPGFSLRSGRLSVS
                     AAYSKTEKTKPVSARVAYAGLSFEIGGDRAAYFVDQGGTRRSAVLRSVEKLPGGARLL
                     FQGGAELRALSAQAPAEGFSLSLVPPQSGPVSLHLPYALSGRARLSDKAGSRLLESGG
                     GPYELGLAGASLDAKSGFLVLQGRAGAGRGLVLAKAAPAEGPARPAAERFIAQAPKDP
                     AVFRAEIGAWVDKAWSGLAGSRWDGERLAWRDKDGAARFSEKALAAYLAESYGRGSHV
                     EGLARARAVKAARPAELSYLTVPFLGDTIRRMQALEADDLVEVKRLGALVQAKDPALF
                     EKEGLIHFLMDRSPYALAQDVLRYASEIDPAKLTTRQALGLLACAAESQSYLSEAENP
                     FKSQGAVVDRLVAAIKKSPEGFFLATEEDGSVDLRLSLLAGVRFAELGQAEGKEVLVG
                     VGQSLVEGVLGLSDESGFTPARVSVGPAGLEKTGSLAPEELYPLVSANPYYPREVSFY
                     RDVAPGVWAWTCAPSLKVEATTSRYLFTSSFPVGRSHYMTFYGVKPFANIKLYDIDYS
                     PDAEFEIYDVSGYFYRKTSSALYLKMKHKSESERIELDF"
     gene            2602..4026
                     /locus_tag="PLG14_09140"
     CDS             2602..4026
                     /locus_tag="PLG14_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49334.1"
                     /translation="MQRTTSRASAALPLLACAASLLLFACAGGPKPGKPSGGGRETAE
                     QKQVRELEGAVALMDKGDAQAAADKVAELAAAQPEKAELRLLKAATLVSAGKLPEARA
                     EVEAVLAAAPEDPRALVMAAELARYSGDEKARRAYLDRGLAAAPEDAGVLAAFGELSL
                     DKKEWTKAEGYFRRARAADPRNADAALGLGKALYRQAKYADSEAAYGEAVALEPKLPF
                     AYADRARARYQLGKYEEAMADLDAAAARAPDSSWIYLDRGRMNLDLERRDRAEADLTR
                     SISLDPEYFLPYVYRAGMYEEAGRDAEALADYRRIIQLQPDYWYALESAGAVAFRLGL
                     WEESAARFREAFGFAPERYEYAILSSISLLRAGKPAEAKALAGKVAPGIDREKNGIYW
                     LMLRLLQDQNDQSSELEIKIQSEKRLDVKAGLLFYLGEYWIGRGKPEIGVKYLVLAKE
                     MRREDCLEHRLLGAELKRLAGANG"
     gene            4019..5080
                     /locus_tag="PLG14_09145"
     CDS             4019..5080
                     /locus_tag="PLG14_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOX49335.1"
                     /translation="MAKPCAEGGARFGRLAALLDRDRPVLVQTHDYPDIDAVASAWGL
                     AGLLGRRGFSAECSYRGKIRARSLSRLVAELGLPLLEEPTPFAGTAAGGTGGAAAGGR
                     AAGTDGVGGAARQIVVVDGSPANGNVSLYEGELVGVIDHHCKSAEPEAPFVDLRPELA
                     SCSAIVHGYWAEAGENPPRDLATALLAGIQSDTDFLSRRASPEDFAAYAALFGTGDFE
                     KASRIVRTVLDLRELGLVVRALESSELREGLLWACLPGPSGQEVLAVLAEFVLRAEEI
                     RAAVVMERGEGGVHLSVRSKDPALSAFALVRSALQGLGAGGGHSHSAGGFVPDAAFPG
                     EGRLRERFFAGVREIARNR"
     gene            5092..>5727
                     /locus_tag="PLG14_09150"
     CDS             5092..>5727
                     /locus_tag="PLG14_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HOX49336.1"
                     /translation="MSKTVRRVELGDRAVLLVGTAHISPESIEEVRSLIASERPTRVC
                     VEIDEGRWKSMSEGQDWASLDIGKVLREGKGFLLMANLALSGFQRRMGSEVGVKPGEE
                     MLAAARAAEELGVPYAFCDREVQLTLKRAWGKSNLWNRSKLIASLASSAFTNEKLSEA
                     EIEQLKERSELEEMMGELADFLPSVKEVLIDERDRYLATKIFLEAKRPLPEA"
BASE COUNT          851 a         2082 c         2048 g          746 t
ORIGIN      
        1 ggaggccacg tcgatccgct cgggctcggc ggggtcggag aggcgcacct cggggtagag
       61 ctcgggaagg cgtgcgcccg aggcccactc ggcccgcgag aggaagacgg cggactggac
      121 cagggaattc cagaggggcg cgaaggcgcg ctcgacctcc ccctttaaaa cggcgcgctc
      181 cccggggctt ccggggagcg cgagggcgta gagccgcgag gtctgcacga cgcggccctc
      241 cacggtttcc ggccccaggc cgatcgcgag ggcctccctg gccgtcagcg cgagggtgtc
      301 cgtcacgccg ggcctgtagg ctatctccac gagccagtcc cagggcccca ggcccggatc
      361 cagcggggcc ggcccctcgg ccaggacctg gaccaccggg tcgccgaaaa gctcggcggc
      421 cgcgtcccga ggcacgggga agtcggtgag gacgacgtcg acgatcctga cggagagggg
      481 ggagggaagg acgcgcccga ggcgggaggc gaggcttccg ccgcgtccgt cgagctccgg
      541 gcgggcgaaa taggtctcta tgcgcatgtc gcacgataca gaatggcggc ggcctcgtca
      601 agccgcggcc cgcccggcgc cgcgcctgac gcgggcgccg cgggcgggcc gccccgggcc
      661 gcgccggctg tccaggctag aagtcgagct cgatccgctc gctctccgac ttgtgcttca
      721 tcttgaggta gagggcgctc gaggtcttcc ggtagaagta gcccgacacg tcgtagatct
      781 cgaactcggc gtcggggctg tagtcgatgt cgtagagctt gatgttcgcg aagggcttca
      841 cgccgtagaa ggtcatgtag tgggagcgcc cgaccgggaa ggacgaggtg aagaggtagc
      901 gcgaggtggt ggcctcgacc ttgagggagg gcgcgcaggt ccaggcccag acgccgggcg
      961 ccacgtcgcg gtagaaagag acctcgcggg gatagtaggg gttggcggag acgagggggt
     1021 agagctcctc gggggcgagg ctgcccgtct tctccaggcc ggcggggccg acggagaccc
     1081 gggcgggcgt gaagccgctc tcgtcggaga ggcccaggac gccctcgacc aggctctgcc
     1141 ccacgccgac caggacctcc ttgccctcgg cctggccgag ctcggcgaag cggacgcccg
     1201 cgagtaggga gaggcgcagg tcgacggagc cgtcctcctc ggtcgcgagg aagaagccct
     1261 ccggggattt cttgatggcg gcgacgagcc tgtcgacgac ggcgccctgg ctcttgaagg
     1321 gattctcggc ctcggagagg taggactggg actcggcggc gcaggcgagg aggcccaggg
     1381 cctgcctcgt cgtgagcttg gcgggatcga tctccgaggc gtagcggaga acgtcctggg
     1441 ccagggcgta gggcgagcgg tccatgagga agtggatgag gccttccttc tcgaagaggg
     1501 ccggatcctt cgcctgcacc agggcgccaa gccgcttgac ctccacgagg tcgtcggcct
     1561 ccagggcctg catgcgcctg atcgtgtcgc ccaggaaggg caccgtcagg tagctgagct
     1621 cggccgggcg cgcggccttg accgcccggg cccgcgccag gccctcgacg tgggagccgc
     1681 ggccgtagga ctcggccagg taggcggcca gggccttctc ggagaagcgg gccgctccgt
     1741 ccttgtcgcg ccaagccagg cgctccccgt cccagcggct gcccgccagg ccggaccagg
     1801 ccttgtcgac ccaggcgccg atctcggcgc ggaagacggc cggatccttg ggcgcctggg
     1861 cgatgaagcg ctcggcggcg ggccgggccg gcccctcggc gggggcggcc ttcgcgagga
     1921 ccaggcccct gcccgcgccg gccctgccct ggaggacgag gaagccggac ttcgcgtcca
     1981 gggacgctcc cgcgaggccg agctcgtagg ggccgcctcc cgactcgagg aggcgggagc
     2041 ccgccttgtc ggacaggcgc gccctgccgg agagcgcgta gggaagatgc agggagacgg
     2101 gtccggactg gggagggacg agggagagcg agaagccctc ggcgggcgcc tgggcggaga
     2161 gggcgcggag ctcggccccg ccctggaaga ggaggcgggc cccgccgggc agcttctcca
     2221 cggagcggag gacggcggag cgcctggtcc cgccctggtc cacgaaatag gcggccctgt
     2281 cgccgccgat ctcgaaggag aggccggcgt aggccacgcg cgcggagacc ggcttcgtct
     2341 tctccgtctt cgagtaggcc gcggagactg agaggcggcc cgagcggagg gagaagcccg
     2401 gcgctcccga gaattggatg aggacgagga ggacgaagat gccgatatag aggagcgtcg
     2461 tgccgatggc gcgtttgtag aagcctgagt tcatcgcggc taccgactat agccggttac
     2521 ggcccccgat gcaaccttgt ataatggctc ctaaaagtat agcatctggc ggcgcccgaa
     2581 cgcctccacg gagagaaaga catgcagaga acgacgtccc gcgcatcagc cgctcttccc
     2641 ctcctcgcct gcgccgcgag cctcctcctc ttcgcctgcg ccggcggccc caagcccggc
     2701 aagccctccg gcggcggccg cgaaaccgcc gagcagaagc aggtccgcga gctcgagggc
     2761 gcggtcgccc tcatggacaa gggcgacgcc caggccgcgg ccgacaaggt cgccgagctc
     2821 gccgccgccc agcccgagaa ggccgagctc aggctcctca aggccgccac cctcgtctcc
     2881 gcagggaagc tccccgaggc cagggctgag gtcgaggcgg tcctcgccgc cgcgcctgag
     2941 gatccgcggg ccctcgtgat ggccgccgag ctcgcgcgct actccggcga cgagaaggcg
     3001 cgccgcgcct acctggaccg gggcctggcc gccgcgcccg aggacgcggg agtcctcgcc
     3061 gccttcggcg agctcagcct ggacaagaag gaatggacga aggccgaggg ctatttccgc
     3121 cgcgcccgcg ccgccgatcc ccggaacgcc gacgcggccc tgggcctcgg caaggccctc
     3181 taccggcagg ccaagtacgc cgactccgag gccgcctacg gcgaggccgt cgccctcgag
     3241 cccaagctgc ccttcgccta cgccgaccgc gcccgcgccc gctaccagct cggaaagtac
     3301 gaggaggcga tggccgacct ggacgccgcc gcggcgcgcg cccccgactc ctcctggatc
     3361 tacctggacc gcggccgcat gaacctggac ctggagcgcc gcgaccgggc ggaggccgac
     3421 ctcacccgct cgatctccct cgaccccgag tacttccttc cctacgtcta ccgcgcgggc
     3481 atgtacgagg aggcgggcag ggacgccgag gccctcgccg actaccgccg catcatccag
     3541 ctccagcccg actactggta cgccctggag tcggccggcg ccgtcgcctt ccgcctcggc
     3601 ctctgggagg agtccgccgc ccgcttcagg gaggccttcg gcttcgcccc ggagcgctac
     3661 gagtacgcca tcctctcgtc catctccctc ctccgcgccg gcaagcccgc ggaggccaag
     3721 gccctggccg gcaaggtcgc cccgggcatc gaccgggaga agaacggcat ctactggctc
     3781 atgctccgcc tcctccagga ccagaacgac cagagctccg agctcgagat caagatccag
     3841 tcggagaagc gcctggacgt gaaggccgga ctcctcttct acctgggcga gtactggatc
     3901 ggccgcggca agcccgagat cggcgtgaag tacctcgtcc tcgccaagga gatgcgccgc
     3961 gaggactgcc tcgagcaccg gctcctgggc gccgagctca agcgcctcgc cggggccaat
     4021 ggctaagccc tgcgcggagg gcggagcccg cttcgggcgc ctcgccgccc tcctcgacag
     4081 ggaccggccc gtcctcgtcc agacccacga ctatccggac atcgacgccg tggcctccgc
     4141 ttggggcctc gccggcctcc tcgggcggcg aggcttctcg gccgagtgct cctaccgcgg
     4201 caagatccgc gcccggagcc tctcccgcct cgtcgccgag ctcggcctcc ccctcctgga
     4261 ggagcctacg cccttcgcgg gaaccgcggc cggcggaacg ggcggcgcgg ccgcgggcgg
     4321 ccgggcggcc ggaaccgacg gcgtgggcgg cgccgcccgg cagatcgtcg tcgtcgacgg
     4381 ctcccccgcg aacggcaacg tgagcctcta cgagggcgag ctcgtcggcg tcatcgacca
     4441 ccactgcaag tcggccgagc ccgaggcgcc cttcgtcgac ctccggcccg agctcgcctc
     4501 ctgctccgcg atcgtccacg gctactgggc cgaggccggc gagaacccgc cccgcgacct
     4561 ggcgacggcc ctgctggccg gcatccagtc ggacacggac ttcctctcgc gccgcgcctc
     4621 tcccgaggac ttcgccgcct acgccgccct cttcgggacc ggcgacttcg agaaggcgag
     4681 ccgcatcgtc cgcaccgtcc tcgacctccg ggagctcgga ctcgtcgtcc gagccctgga
     4741 gtcctccgag ctccgcgaag gcctcctctg ggcctgcctg cccggcccct cgggccagga
     4801 ggtcctggcc gtcctcgcgg agttcgtcct ccgcgccgag gagatccgcg ccgccgtcgt
     4861 catggagcgc ggcgagggcg gcgtccacct ctccgtccgc tcgaaagacc cggccctctc
     4921 ggccttcgcc ctcgtgcgct ccgccctcca gggcctcggt gccgggggcg gccattcgca
     4981 ctccgcgggg ggcttcgtcc ccgacgcggc cttcccgggg gaggggcggc tccgcgagcg
     5041 cttcttcgcc ggcgtcaggg aaattgcccg gaacagataa gggagcatcg catgtccaag
     5101 accgtccgcc gggtggagct cggcgaccgc gccgtcctcc tcgtgggcac ggcccacatc
     5161 tcgcccgaaa gcatcgagga ggtgcgctcg ctcatcgcct ccgagcgccc gacgcgcgtc
     5221 tgcgtggaga tcgacgaggg ccgctggaag tcgatgagcg agggccagga ctgggccagc
     5281 ctcgacatcg gcaaggtcct ccgcgagggc aagggcttcc tcctcatggc caacctcgcc
     5341 ctctcgggct tccagcgccg catgggctcg gaggtcggcg tgaagcccgg cgaggagatg
     5401 ctggccgccg cccgcgccgc cgaggagctc ggcgtcccct acgccttctg cgaccgcgag
     5461 gtccagctca ccctcaagcg ggcctggggc aagtccaacc tctggaaccg ctccaagctc
     5521 atcgccagcc tcgcctccag cgccttcacg aacgagaagc tctcggaggc cgagatcgag
     5581 cagctcaagg agcgcagcga gctcgaggag atgatgggcg agctcgccga tttcctgccc
     5641 tccgtgaagg aggtcctcat cgacgagcgc gaccgctacc tcgcgaccaa gatcttcctg
     5701 gaggccaagc gcccgctccc ggaggcg
//
LOCUS       DAOOSJ010000180         5608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_8994_length_5608_cov_4.341797, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000180 DAOOSJ010000000
VERSION     DAOOSJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8994_length_5608_cov_4.341797"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLG14_09155"
     CDS             <1..509
                     /locus_tag="PLG14_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOX49337.1"
                     /translation="GGQFSVSPGGHFLMSPDSIAFKNGKLLMIESKMAGDYKFPGGGV
                     EKGETEFDALIREVREESGHNVSKIGHIVFRIIETSKDKELDNTKFKMVSDYYLCEVD
                     DGVDSLSLDDYEREMGFSPIWIGIEEAVKKNIEVSKSEKCTKWVERELLVLNEITNRK
                     ISLTIASS"
     gene            633..1166
                     /locus_tag="PLG14_09160"
     CDS             633..1166
                     /locus_tag="PLG14_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49338.1"
                     /translation="MREYFEMSLDDLREVAAWAAGFAERALAVYEERVPGDARPRLAL
                     EGAKEFARGGGRTNALRKLAMDAYRASREAVDGAASEAANAASLAAALPFTHPFRDLR
                     QAEHLLGPVVHAALALEDGALAGKGEALLDEAARAAGGAVAGLLRNYPRREPGSTRRD
                     EAFHRLDSAILGFASGR"
     gene            complement(1197..1763)
                     /locus_tag="PLG14_09165"
     CDS             complement(1197..1763)
                     /locus_tag="PLG14_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOX49339.1"
                     /translation="MARPRVAIYLALSLDGFIAREDGSLGWLERVQAEGEDYGYAAFF
                     AGIDAVLLGRKTFDTVLGFGAWPYEGKAVYVLSSRALGELPGFVPRDAAGPARVSGPI
                     EAVLGLISSEGRLSVYLDGGETARQALAAGLVDEMTLSIVPTLLGRGRPLFGPELPFS
                     EWELLSARSFPSGLVQARYAPAKTAPRG"
     gene            complement(1825..5259)
                     /locus_tag="PLG14_09170"
     CDS             complement(1825..5259)
                     /locus_tag="PLG14_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX49340.1"
                     /translation="MPSTHTAPAALAPGREGVASGEYPLIAAEKFEAALSRVSRVHER
                     FSRELGSMAEALAQEAGLADDCASLASRLPEGIEAIGEAARDIERALAAVEAAIEAGR
                     ASLEDVVEDVRDEIAGLEERTAGMRDIADFGARVSEGAGRVAEIADENRLIAINASVS
                     ASKAGDRVRGFKVIAAEISRLSGAMAARVPEVIRASAQVASRMDQIMAGMDESIAGAR
                     RALGAIDEAFSRLAAIGGALREASSGAAAMLEESGALAEGGRAIDAALGSMRDAIALS
                     RDGAAALSSQMGAQGSAIARLGERMPSVTGLARGIAAGGAAAGGGGTAEGPKILRMNE
                     IGLDRYDPALTRMIREIHYTSFTCIRLLRYSSEKKIVPYLAETWFLHPDGRTWEFALR
                     RDARFSDGSPIGTADVRFSFERLLNPALGSPYAQLFTVIEGAEEYRSGAEPSVRGIET
                     PDALTLRIRLREPNNFFLSLLALGYSAVVKANPAYAYRPISKEELVSAGPFAPAPDPE
                     PGVDRLVANPGFVNGRPFIDEIRIRRTDAPPIEELLAGRVDLAYNLTAASKRALESGG
                     FEGGFRPYTSRYCYGLVVNFARSSALARSPELRLALAMGLDKEGIIREALGGAGLRAD
                     CVIPSSLVDSGGEPFVPYDPEGARARIAAALRASAGRAAAPIKIAIREYPTIAGLERL
                     GPAIQSNLERLGLSATVDYHPLSKPIGSYREAYDLTFIGFLPEIDLYSAVEPFINPEG
                     GDNYFGYANEALFKELDATRGVKDEGERRARFSRILKTLTEDCFMVPIFFQQVYCAFR
                     RGIRGVYVAAEETVMPELLFMEPEPAAGAAGPRDRQGDGPRGPEAVLGAAGTLGDYAA
                     AVGALEVEATAVLDGSRALIDRSASIESGVDRQRPAVDKANGLFAAFAEGAGRVEAGK
                     ARLGALIATSSAEASTAADAAKGVAAGLADLGATLQATTKSLASVLAEVDGMLDTLGA
                     IRSSNEFITSIAVNAAIIAAKSDRGAGDLRKVSQSIAAQAKRNTEHTEGLAKTVDEMR
                     AAVLDHQTFLAATLAALERSAAGAGAAGSAMAGALPLLGEVSSGGERAALATAGLGSL
                     VAEERKALAAITDKAETLSQAAETLRFGMDLERAVADILADVGVINRDVRDFAEGN"
BASE COUNT          845 a         1857 c         2020 g          886 t
ORIGIN      
        1 aagggggtca attctcagtg tcgcctgggg gtcattttct catgtcgcct gacagcattg
       61 cctttaaaaa tggcaaactg ttgatgattg aatcaaaaat ggcaggtgac tacaaattcc
      121 caggtggcgg agtggaaaaa ggcgaaaccg aatttgatgc gttgattagg gaagtgagag
      181 aagaaagtgg ccataatgta tcgaaaatag gtcatattgt attcaggata atcgaaacca
      241 gcaaagacaa agaactggat aatacaaaat tcaagatggt ttctgactac tatttgtgtg
      301 aagttgatga tggagtcgat agcttgagtt tggacgacta tgaaagagaa atgggattca
      361 gcccaatatg gattggcata gaagaggccg ttaagaagaa cattgaagta tcgaagagtg
      421 agaagtgcac caagtgggtc gaaagagagt tgctggtgtt gaatgaaata acaaatagga
      481 agatttcgct aacgatcgct tcatcctgac tccgccgcct gtcacggttt gtgcttcagt
      541 cgcttcgctc caggcacaaa ccgcgccggg ctcgcgtcgc cggttgagcg tttgatttgc
      601 cgacggagag gccgagccgc ggaaggattc ctatgcgcga gtatttcgag atgagcctgg
      661 acgatctgcg cgaggtcgcc gcctgggcgg cgggtttcgc cgagcgggcc ttggcggtct
      721 acgaggaacg cgtgcccgga gacgcgaggc ctcgcttggc cctggaaggc gcgaaggagt
      781 tcgcgcgcgg cggcgggcgg accaacgcct tgcggaagct cgcgatggac gcctatcgcg
      841 cctcgcggga ggcggtggac ggggcggcct ctgaggcggc gaacgcggcc agcctggccg
      901 cggcgctccc cttcacccat cccttcaggg acctgcggca ggcggagcac cttctcggtc
      961 ccgtggtcca cgccgccctc gccctggagg atggcgccct ggcgggcaag ggcgaggccc
     1021 tgctggacga ggccgcgcgc gcggcgggag gggcggtcgc cggcttgctg cggaactatc
     1081 cgaggcgcga gcccggctcc acgaggcggg atgaggcctt ccataggctc gattccgcca
     1141 ttctcggctt cgcgtccggt cggtaggccc cgctgtcccg ggcgcggcgg ggcgcgtcat
     1201 ccgcggggcg cggtcttggc aggcgcgtac ctcgcctgga ccagcccgct cgggaaggag
     1261 cgggcgctca acagttccca ttcggagaag ggcagttccg ggccgaacag cggccggccg
     1321 cggccgagca gggtggggac gatggagagg gtcatctcgt ccacgagccc cgcggccagg
     1381 gcctggcggg cggtctcgcc gccgtccagg tatacggaga gcctcccctc cgagctgatc
     1441 agcccgagca cggcctcgat gggcccggag acccgcgccg gccccgccgc gtcccgcggg
     1501 acgaaaccgg gaagctcgcc caatgcccgg cttgagagga catagaccgc cttcccttcg
     1561 tagggccagg cgccaaaacc gagcacggtg tcgaaggtct tccggcccag gaggacggcg
     1621 tcgattcccg cgaagaaggc cgcgtagccg tagtcctcgc cctccgcctg gacgcgctcc
     1681 agccagccga gcgagccgtc ctcgcgggcg atgaagccgt caaggctgag ggccaggtag
     1741 atcgcgactc ggggacgggc catgggatcc tcctcgtgcc tgccctgggc ttgcggccgc
     1801 cggctgggcg ggccttcctc gcccctagtt cccttcggcg aagtcgcgga cgtcgcggtt
     1861 gatgacgccc acgtcggcca ggatgtcggc cacggcccgc tccaggtcca tgccgaagcg
     1921 cagggtctcc gccgcctggc tcagggtctc ggccttgtcc gtgatggccg cgagggcctt
     1981 ccgctcttcg gccacgaggc ttccgagccc ggccgtcgcc agggcggccc gttcgccgcc
     2041 tgaggagacc tcgccaagga gcggcagggc tccggccatg gccgagccgg cggctcctgc
     2101 cccggcggcc gagcgctcca gggccgcgag ggtcgcggcc aggaaggtct ggtggtccag
     2161 cacggccgcg cgcatctcgt ccacggtctt ggccaggccc tcggtgtgct cggtgttgcg
     2221 cttcgcttgc gccgctatgg actggctcac cttgcgcagg tcgcccgcgc cccggtcgct
     2281 cttggccgcg atgatggccg cgttgaccgc tatcgacgtg atgaactcgt tggacgagcg
     2341 gatggccccg agcgtgtcga gcatcccgtc gacctccgcc aggacggagg ccaggctctt
     2401 cgtcgtcgcc tggagggtcg cgcccaggtc ggcgaggccg gccgccacgc ccttggccgc
     2461 gtccgcggcc gtggaggcct cggccgagct cgtggcgatc aaggccccga ggcgggcctt
     2521 gcccgcctcg acgcgcccgg ctccctcggc gaaggccgcg aagaggccgt tggccttatc
     2581 caccgccggg cgctgccggt ccacgccgga ttctatcgaa gccgagcggt cgatcagggc
     2641 ccgggatccg tccaggacgg ccgtcgcctc gacctccagg gctccgacgg cggcggcgta
     2701 gtcgcccagg gttccggcgg cgcccaggac ggcctccgga ccgcgcggcc cgtccccctg
     2761 cctgtcccgc ggcccggccg cccccgcggc gggctcgggc tccatgaaga ggagctccgg
     2821 catgacggtc tcctccgcgg cgacgtacac gccgcggatg ccccgcctga aggcgcagta
     2881 cacctgctgg aagaagatcg ggaccatgaa acagtcctcg gtcagggtct tgaggatgcg
     2941 gctgaagcgc gcgcgccgct cgccctcgtc cttgacgccc ctggtcgcgt cgagctcctt
     3001 gaacagggcc tcgttggcgt agccgaagta gttgtcgccg ccttcggggt tgatgaaggg
     3061 ctccacggcc gaatagaggt cgatctccgg caggaagccg atgaaggtga ggtcgtaggc
     3121 ctcgcggtag ctcccgatgg gcttcgacag ggggtggtag tccaccgtgg cggaaaggcc
     3181 caagcgctcg aggttggact ggatcgcggg gcccagcctc tccaggccgg cgatcgtcgg
     3241 gtactctcgg atggcgatct tgatcggggc cgcggcgcgg ccggcggacg cgcggagggc
     3301 ggccgcgatc ctcgccctcg cgccctcggg gtcgtagggc acgaagggct ctccgcctga
     3361 gtcgacgagg gaggagggga tgacgcagtc ggccctgagg cccgcgccgc ccagcgcctc
     3421 gcggatgatg ccctccttgt ccaggcccat ggccagggcc agcctgagct cgggcgatcg
     3481 ggcaagggcg gaggaccgcg cgaagttgac cacgagtccg tagcagtagc gcgaggtgta
     3541 gggcctgaag ccgccctcga agccgcccga ctccagggcg cgcttggagg ccgcggtcag
     3601 gttgtaggcc aggtcgacgc ggccggccag gagctcctcg atcggcgggg cgtcggtcct
     3661 gcgtatgcgg atctcgtcga tgaagggccg gccgttgacg aagcccgggt tcgcgaccag
     3721 ccggtccacg ccgggttcgg gatcgggcgc cggggcgaag ggtccggcgg agacgagctc
     3781 ctccttggag atggggcggt acgcgtaggc cggattcgcc ttgaccacgg ccgagtagcc
     3841 cagggccagg aggctcagga agaagttgtt gggctcgcga aggcggatgc gcagcgtcag
     3901 ggcgtccggc gtctcgatgc cgcgcacgga aggctcggcg cccgaccggt actcctccgc
     3961 tccctcgatg acggtgaaga gctgggcgta gggggagccc agcgccggat tcaggagccg
     4021 ctcgaaggag aagcggacgt ccgcggtgcc gatggggctc ccgtcgctga agcgcgcgtc
     4081 ccggcgcagg gcgaactccc aggtcctgcc gtcggggtgc aggaaccagg tctccgcgag
     4141 gtagggcacg atcttcttct cgctcgagta gcggaggagg cgtatgcagg tgaagctcgt
     4201 gtagtggatc tcgcggatca tgcgggtcag ggccgggtcg tagcggtcca ggccgatctc
     4261 gttcatacgc aggatcttgg ggccctcggc cgtcccgccg ccgcccgcgg ccgcgccgcc
     4321 cgcggcgatg ccgcgcgcga gcccggtcac cgagggcatg cgctcgccaa ggcgcgcgat
     4381 cgcgctcccc tgcgcgccca tctgggagct cagggcggcc gctccgtcgc gcgagagggc
     4441 tatcgcgtcg cgcatcgacc cgagcgcggc gtctatggcg cgtccgccct cggccagggc
     4501 cccgctctcc tcgagcatag cggccgcccc ggaagaggcc tcgcgcaggg ccccgccgat
     4561 ggcggcgagg cgcgagaagg cctcgtcgat cgcgcccagg gcccggcggg cgccggcgat
     4621 gctctcgtcc atccccgcca tgatctggtc catccgcgag gccacctggg cggaggcccg
     4681 gatgacctcg ggcacgcggg ccgccatggc tccggagagg cgcgatatct cggccgcgat
     4741 gaccttgaag cccctgaccc ggtcgccggc cttgctggcg ctgaccgagg cgttgatggc
     4801 gatgaggcgg ttctcgtccg ctatctcggc gacccgcccc gcgccttcgc tcacgcgggc
     4861 gccgaagtcg gcgatgtcgc gcatgccggc ggtgcgctcc tcgagccccg cgatctcgtc
     4921 cctgacgtcc tcgacgacgt cttccaggct cgcgcgcccg gcctctatgg cggcttcgac
     4981 ggcggccagc gcgcgctcga tgtcacgcgc ggcctcgccg atggcctcga tcccctcggg
     5041 cagtctggag gcgagggatg cgcaatcgtc ggccagtccc gcctcctggg ccagggcttc
     5101 ggccatcgat cccagctccc ggctgaagcg ctcatggacc cgcgagacgc gggaaagggc
     5161 ggcctcgaat ttctcggccg cgatgagggg atactcgccg gatgctaccc cttcgcggcc
     5221 gggagcgagg gcggcgggcg ccgtatgggt ggaaggcatg tcagtactct actatcgagc
     5281 gcgcctatgg acaaggcgcg gaaaaccccg ctggcggctc tggcgcggcg gcctcgggct
     5341 gcgcccgggc gggccgtcag gagcgaggcc tttcagcgcc gtcttgacgc tccccctctt
     5401 ttcctatact tgtcgcatat catcaaaccc gggagcgctc gccgtcatga atatccttgg
     5461 ggtcaaccac atcaccatcg ccgtctcgga cgtggaggag gccttccggt tctacacgga
     5521 gcttctcggc ctcgcgcccg tcatgaagag cgaggtggcc gcctatgtct cggcggccgg
     5581 cgcctggata gccctggtcg cggacgag
//
LOCUS       DAOOSJ010000181         5546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9131_length_5546_cov_5.124567, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000181 DAOOSJ010000000
VERSION     DAOOSJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_5546_cov_5.124567"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLG14_09175"
     CDS             <1..505
                     /locus_tag="PLG14_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOX49341.1"
                     /translation="SGSLVTAGGGALLYAAARRDGTVLRNLAESLLPELRMTDFNAAL
                     GFAQLKELEASIAKRRELYGAFSQSLARTRHGLLSQEGEGEPAYWAFPVLLSSGMKDV
                     RAYAKKKEVDTEPAFEGSLVGRGLVPEGSCPNARSVLMRALLFPLHQRVGQSGAQKIS
                     RVLATLP"
     gene            520..1371
                     /locus_tag="PLG14_09180"
     CDS             520..1371
                     /locus_tag="PLG14_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOX49342.1"
                     /translation="MADRKVLIIANLLKDDASGVAKEMAAHLRTLGVSAEIFAFSGPP
                     GVNPSAEGLDLVVTLGGDGTVLFAARSAALHGVPVLPFNLGRLGFIAGFGKSDWAVAL
                     DLWLAGRIGLTARSMLAVEVRREGQAVASFDALNDGVVSAQGIAKVIGLDIRVGGSHL
                     GSYRSDGVIVATPTGSTAYNLAAGGPVLHPEMDAMILNPICPFTLSNRPLVVPGSERI
                     DVLIEEGKRAAVMLTVDGQVTFDLKAGDRVYFTHGKRRALIYTPERLAFYDVLREKLS
                     WSGGPNA"
     gene            1364..3067
                     /gene="recN"
                     /locus_tag="PLG14_09185"
     CDS             1364..3067
                     /gene="recN"
                     /locus_tag="PLG14_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOX49343.1"
                     /translation="MLEELSVRDFALIDRLSVSFSGGLSILSGETGAGKSLLIGAIGF
                     LLGGKADTGVIRTGAEEALVTGLVDVSASPEAQAWLAARGIEAEEGRVLVRRGIKANG
                     RGSIFIQNQPALRADLSELTGLLVDLHGQHEHQSLLASENHRRILDRYAGLEAEVVEF
                     GLRFAELSSKRRSYESALASEKERARETEFLRYAVEEIGKAKLKRGEEAELEAEERLL
                     GQHEKLYAAVAEAHEALSRADESGGILSSLRRGRSALEAAQAIDPALADLSRRSDDAY
                     YELEDVADSLRSYLGGLRYSPERLEEIESRLAELHKLKKKYGGSVEDLLSRLAEDQER
                     LSRLETWEEDKEGLAAEIAALESSILAAALALSEKRKKAAGSLETRIEAILRTLGMPR
                     SRFLVRMGRKEPEGGKPVVGQHGVDEIEFLIAPNPGEPPKPLSRIASGGELSRVALAA
                     KTVLAETDTVDTLIFDEIDAGIGGEVAVAVGEHLKALGSRRQVLCITHLASIAARADN
                     HYRVEKEVADGRTSTRLARMEGRPRAEEIARMLAGDPEAEASVAHATELLRKYGNWRD
                     G"
     gene            3067..3873
                     /locus_tag="PLG14_09190"
     CDS             3067..3873
                     /locus_tag="PLG14_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49344.1"
                     /translation="MGKITQEQRNRYFEMVKEYRKTIEAGLAKEKTLLELLGKEESGA
                     AYKRLFLAETVLDLSSWYVLVNGLSVSLLGLKNEDYLLEGRKTLVRALKYLEDTVTAT
                     LDGPFSDYEDRLAEIKDYDAASRYRLARKFGFAVQSLEEAFGENSKYSWSFLEIWGKF
                     AAVSKNLVDLKSAIADLDFNSPTRPVVMGHLALVKGQLQRCADRYREQYELYTHKHQD
                     FKQAIAYLSALRRLHMNLGERDDAETMKKKADVWNSKLEADSKKQEEKKR"
     assembly_gap    3879..3978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4021..4452)
                     /gene="cdd"
                     /locus_tag="PLG14_09195"
     CDS             complement(4021..4452)
                     /gene="cdd"
                     /locus_tag="PLG14_09195"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOX49345.1"
                     /translation="MLSTHGAEEHMDKAKLFALASDAARASYSPYSKFRVGAALLCAD
                     GTVFTGTNVENRSFGLTICAERSALVAAIGAGKREFEGIAISTPDADYPVSPCGACRQ
                     VLSEFLKPSAPVVFGSKAGTLVETTIGDLLPNDALHELGSR"
     gene            4526..>5546
                     /locus_tag="PLG14_09200"
     CDS             4526..>5546
                     /locus_tag="PLG14_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49346.1"
                     /translation="MEDTGKRRLAAAAVALAGLVYFFVCARPLPKALVLEPRWARTLG
                     AASGEQAAPAAGQPRPAASTATAAASSVAPAAGAPVAAGSAGLAAAAPLSASPQGGFD
                     ADSGWIPYRIAGNYGYFDEEGKVALKAASPFGVALAKGAYVAYDRLPESLLLRSASGR
                     ELARVAEAGYPFFSAGRLFVMRPGQSSVAEIGPDGKALWSRDFPSIVTAFDASPGLAL
                     FGLMDGSLVGVGADGAELLSFAPGGSRVAGVYGCAVSPDGLMAAAVTGLDRQRLVVLE
                     RRSSAFRVTYHRWLESDFRRQVSMAFTEDGRYLAYEIPSGLGVYDREKRAESKIASAA
                     LSGVGL"
BASE COUNT          835 a         1995 c         1817 g          799 t
ORIGIN      
        1 gtctggctcc ctcgtgacgg ccggcggcgg cgccctcctc tacgcggccg cccggcgcga
       61 cggcacggtc ctgcgcaacc tggccgagtc cctccttccc gagctcagga tgaccgactt
      121 caacgcggcc ctgggcttcg cccagctcaa ggagctcgag gcgagcatcg ccaagcggcg
      181 ggagctttac ggcgccttct cgcagtccct cgcgcggacc aggcacggcc tcctctccca
      241 ggaaggcgag ggcgagccgg cctactgggc cttccccgtc ctcctctcct cggggatgaa
      301 ggacgtcagg gcctacgcga agaagaagga agtggacacc gagccggcct tcgagggcag
      361 cctcgtgggc cggggcctcg tccccgaagg ctcctgcccg aacgcccgct cggtcctgat
      421 gcgggccctc ctctttcccc tgcaccagcg cgtcgggcag tccggcgcgc agaagatctc
      481 cagggtgctg gcgacgctcc cgtagaggca tgaggcgcca tggccgatag gaaggtcctc
      541 atcatcgcca atctcctcaa ggacgacgcc tccggcgtcg cgaaggagat ggccgcgcac
      601 ttgcgcacct tgggagtctc cgcggagatc ttcgccttct cgggcccgcc gggggtgaac
      661 ccgtccgccg agggcctgga tctcgtcgtc accctgggcg gcgacggcac cgtgctcttc
      721 gccgcgcgct ccgccgccct ccacggcgtc cccgtccttc ccttcaacct cggccgcctg
      781 ggcttcatcg ccggcttcgg caagtccgac tgggcggtcg ccctcgatct ctggctcgcg
      841 gggcggatcg gcctcacggc caggtcgatg ctcgccgtgg aggtcaggcg cgagggccag
      901 gccgtcgcct ccttcgacgc cctcaacgac ggcgtcgtct cggcccaggg catcgcgaag
      961 gtgataggcc tggacatcag ggtcggcggc tcccacctgg ggagctaccg ctcggacggc
     1021 gtcatcgtcg cgacgcccac gggctcgacc gcctacaacc tggcggccgg cggccccgta
     1081 ctccatcccg agatggacgc gatgatcctg aatcccatct gccccttcac cctctccaac
     1141 cggccgctgg tcgtgccggg cagcgagagg atcgacgtcc tcatcgagga gggcaagcgc
     1201 gcggccgtga tgctcacggt cgacggccag gtgaccttcg acctcaaggc cggcgaccgg
     1261 gtctacttca cgcacggcaa gcgccgcgcc ctgatctaca ctcccgagcg cctcgccttc
     1321 tacgacgtgc tccgcgagaa actcagctgg tcgggagggc ccaatgcttg aggagctttc
     1381 cgtacgcgac ttcgccctca tcgaccgcct ctccgtctcc ttctctggcg gcctgtccat
     1441 actctcgggc gagactggcg cgggcaagtc cctcctcatc ggcgccatag gcttcctcct
     1501 gggcggcaag gcggacacgg gggtcatcag gacgggcgcg gaggaggcgc tggtcacggg
     1561 cctcgtggac gtgtccgcga gcccggaggc ccaggcctgg ctcgcggcca ggggcatcga
     1621 ggccgaggag ggcagggtcc tcgtccgccg cggcatcaag gcgaacggca ggggctcgat
     1681 cttcatccag aaccagccgg cgctcagggc cgacctctcg gagctcacag gcctcctcgt
     1741 cgacctccac ggccagcacg agcaccagtc cctcctcgcg agcgagaacc accgcaggat
     1801 cctggaccgc tatgcgggcc tggaggcgga ggtcgtggag ttcggcctgc gcttcgccga
     1861 gctcagctcc aagcggagat cctacgagag cgccctcgcc tccgagaagg agcgcgcccg
     1921 cgagaccgag ttcctgcgct acgccgtgga ggagataggg aaggccaagc tcaagcgggg
     1981 cgaggaggcc gagctcgagg ccgaggagcg cctcctcggc cagcacgaga agctctacgc
     2041 ggccgtcgcc gaggcccacg aggccctctc ccgcgcggac gagtcgggcg gcatcctctc
     2101 ctccctgcgg cgcggccgct ccgccctgga ggcggcccag gccatcgatc ccgcgctggc
     2161 cgacctctcc cggcgctccg acgacgccta ctacgagctc gaggacgtcg ccgactccct
     2221 gcggtcctac ctgggcggcc tccgctacag cccggagcgc ctggaggaga tcgagtcgcg
     2281 cctggccgag ctccacaagc tcaagaagaa gtacggcggc tcggtggagg acctcctgtc
     2341 ccgcctcgcc gaggaccagg agcggctgtc gcgcctcgag acctgggaag aggacaagga
     2401 aggcctggcc gcggagatcg ccgccctcga gtcaagcatc ctggcggccg ccctcgcgct
     2461 ctcggagaag aggaagaagg ccgcgggctc cctggagacg cgcatcgagg ccatcctgcg
     2521 caccctcggc atgccgcgct ctcgcttcct ggtccggatg ggacggaagg agcccgaggg
     2581 cgggaagccg gtcgtgggcc agcacggcgt cgacgagatc gagttcctca tcgcgccgaa
     2641 tcccggcgag ccgcccaagc cgctctcgcg gatagcctcg ggcggcgagc tctcccgcgt
     2701 cgccctcgcg gccaagaccg tcctcgcgga gaccgacacc gtggacaccc tcatcttcga
     2761 cgagatcgac gcggggatcg gcggcgaggt ggccgtcgcg gtcggagagc acctcaaggc
     2821 cctgggctcg aggcggcagg ttctttgcat tacgcatctc gcctccatag ccgcccgcgc
     2881 cgataatcat tacagggtcg agaaggaggt ggccgatggc cgtacctcca cgcgcctggc
     2941 ccgcatggag ggcaggccga gggccgagga gatcgcgcgc atgctcgcgg gcgatcccga
     3001 ggccgaggcc tccgtggcgc acgcgaccga gcttctccgt aaatacggga actggcggga
     3061 cggctgatgg gcaagatcac gcaggagcag cgcaatcgtt acttcgagat ggtcaaggaa
     3121 taccgcaaga ccatcgaggc ggggctcgcc aaggagaaga ccctcctgga gctcctcggc
     3181 aaggaggagt cgggcgcggc ctacaagcgc ctcttcctcg cggagacggt gctcgacctc
     3241 tcctcctggt acgtcctcgt gaacggcctc tccgtctccc tcctgggcct caagaacgag
     3301 gactacctcc tcgagggcag gaagaccctg gtccgcgccc tcaagtacct ggaggacacg
     3361 gtgaccgcca ccctggacgg ccccttctcg gactacgagg acaggctcgc ggagatcaag
     3421 gactacgacg cggcctcccg ctaccggctc gcccgcaagt tcggcttcgc cgtccagtcc
     3481 ctggaggagg ccttcgggga gaattccaag tacagctggt ccttcctgga gatctggggg
     3541 aagttcgcgg ccgtctccaa gaacctcgtc gacctgaaga gcgcgatagc cgacctggac
     3601 ttcaactcgc cgacgcggcc cgtggtcatg gggcacctgg ccctggtgaa gggccagctg
     3661 cagcgctgcg ccgaccgcta ccgcgagcag tacgagctct acacgcacaa gcaccaggac
     3721 ttcaagcagg ccatcgccta tctctccgcc ctgcgccgcc tccacatgaa cctgggcgag
     3781 agggacgacg cggagaccat gaagaagaag gccgacgtgt ggaattccaa gctggaggcc
     3841 gactccaaga agcaggaaga gaagaagcgc taggcggcnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnncc gccgctgccg cgatccccgg agccgcccgg cgccgcgccg
     4021 ctaccgcgag ccgagctcgt ggagggcgtc gttgggcagg aggtcgccga tcgtggtctc
     4081 cacgagggtc ccggccttgg agccgaagac gacgggcgcg gagggcttga ggaactcgga
     4141 gaggacctgg cggcaggcgc cgcaggggct caccggatag tccgcgtcgg gcgtggagat
     4201 cgcgatcccc tcgaactccc tcttgcccgc gccgatcgcc gcgacgagcg cgctccgctc
     4261 cgcgcagatg gtcaggccga aggacctgtt ctccacgttg gtgccggtga agaccgtccc
     4321 gtcggcgcag agcagggccg ccccgacccg gaacttcgag tagggagagt aggaagccct
     4381 cgccgcgtcg gaagcgagtg cgaataactt ggccttgtcc atgtgctcct ccgcgccatg
     4441 agttgacagc aacgagagat agtatatacc attcggccag cagagggaag ctcgcggggg
     4501 cttccggaaa ggccaaagag agctcatgga agatactggc aagaggcggc tcgccgccgc
     4561 cgcggtggcc ctcgccggcc tcgtctactt tttcgtctgc gcgcggcccc ttcccaaggc
     4621 cctggtgctc gagccgcgct gggccaggac gctcggcgcc gcctcaggcg agcaggccgc
     4681 gcccgccgcc ggacagcctc gccccgcggc atccacggcg accgccgcag cttccagcgt
     4741 cgcgcccgcc gctggcgctc cggtcgccgc gggctccgcc ggcctcgccg ccgccgcgcc
     4801 gctctcggct tccccgcaag gcggcttcga cgccgattcg ggctggatcc cctaccgcat
     4861 cgccggcaat tacggctact tcgacgaaga gggaaaggtc gccctcaagg ccgcctcgcc
     4921 cttcggcgtc gccctcgcca agggcgccta cgtggcctac gacaggctcc ccgagagcct
     4981 cctcctccgc tccgcctcgg gcagggagct cgcgcgcgtc gccgaggcgg gctatccctt
     5041 cttctccgcc ggacggctct tcgtcatgcg gccgggccag tcctccgtcg cggagatcgg
     5101 ccccgacggc aaggccctct ggtcgcgcga cttcccctcg atcgtcaccg ccttcgacgc
     5161 gagtccgggc ctagccctct tcggcctcat ggacgggagc ctcgtcggcg tcggcgccga
     5221 cggcgcggag cttctctcct tcgcgccggg cgggagcagg gtcgcgggcg tctacggctg
     5281 cgcggtctcc cccgacggcc tcatggccgc ggccgtcacc ggcctcgacc gccagcgcct
     5341 cgtcgtcctc gagcggcgct cctcggcctt ccgcgtgacc taccaccgct ggctcgagtc
     5401 ggacttccgg cgccaggtct ccatggcctt caccgaggac ggccgctacc tcgcctatga
     5461 gatcccctcc ggcctcggcg tctacgaccg cgagaaacga gccgagtcga agatcgcgtc
     5521 ggcggccctc tccggcgtcg gccttt
//
LOCUS       DAOOSJ010000182         5486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9262_length_5486_cov_4.888050, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000182 DAOOSJ010000000
VERSION     DAOOSJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_5486_cov_4.888050"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PLG14_09205"
     CDS             <1..139
                     /locus_tag="PLG14_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOX49347.1"
                     /translation="DFLCQEMNREVNTIGSKNILLPVAEAVVELKDALENVREQLRNI
                     E"
     gene            153..689
                     /locus_tag="PLG14_09210"
     CDS             153..689
                     /locus_tag="PLG14_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX49348.1"
                     /translation="MGASIVAISGKSGCGNSTVTRLLAERLGFKVVNYTFRNMAADRG
                     LTLARVLELAGQDDSWDRDLDAHQVELARAGDCVIGSRLAMWLLPEAALRVYLWASPE
                     IRAARIHTREGGSLDEVIAFTRKRDEADRARYRRIYGLDNDDYSEADLTVNTERFGPA
                     EIASILAEALARKLAPKA"
     gene            702..1526
                     /locus_tag="PLG14_09215"
     CDS             702..1526
                     /locus_tag="PLG14_09215"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX49349.1"
                     /translation="MERREPVALVTGASSGIGNACATYLAKRGYKVYGTSRNPASYAK
                     KADEYFELVQMDVTDEASVRKAVDEILAKEGRIDALLCNAGMGISGSVEDSSLEEIRL
                     QLETNFLGVVRCVKAVLPGMRQAKAGRILVLSSIAGVIGMPFQAFYSASKFALEGFVE
                     ALRYEAYPFGVQAALIEPGDFRTGFTDARRPVKAAEGSHYKKLYDAAMGVQVRDERAG
                     HDPLEVAKLAVRLLQKRRLKIRYSVGPAFERFSVVLKRRIPDLLFEYGYRKYYKLP"
     gene            1539..2579
                     /locus_tag="PLG14_09220"
     CDS             1539..2579
                     /locus_tag="PLG14_09220"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOX49350.1"
                     /translation="MSIRKTSFGTTAEGFEASLYTLSSGGLEAGLSDFGATLVFLEAP
                     AGRGETVDLVLGLESAAAYEASDAYLGATAGRFANRIAKGRFSLDGKDYGLAANNGPN
                     HLHGGLRGFDKRPWRVEASELGGSPALRFSRLSPDGEEGYPGALSVSATYVLEPEGVL
                     RVLFEAGTDAATLVSLTQHAYFNLRGAGSGDILGHDLELRASRYLPIDKDLIPTGELA
                     PVEGGPFDFRAAKPLGRDIEALGDAAGGYDHCFVLDRDGDGLFEFAELREPLSGRRMT
                     AATTLPGVQLYTGNFLSGAKGRKGQVYARRGGVCLEAELFPDSPNRPAFPSPVLRPGE
                     TWRHETLYRFFL"
     gene            complement(2623..3957)
                     /locus_tag="PLG14_09225"
     CDS             complement(2623..3957)
                     /locus_tag="PLG14_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49351.1"
                     /translation="MRYHCCLALAGLFAVSTLGAQEPAPAGVETAEEAPSEAVFAPFP
                     SKLRAALREGQVVLSWEDSADVKGRYAVYRSLAPIDGLSAFAGAEKVGEVESGIGTYV
                     DRPSGGAAYYYLVLAVGSEGEPYKVFIPARNTTLAAIALPPSESLPAAPAPAAPATPA
                     AATAAEPPSLEGVAAAQAKDAVVVSYRSRIPGQRLVLYRSSQPILGSASLLDAVLVAA
                     FEDKSGAIADYPVPGVDYWYAILPEEELRAGRLSVEPGRNATRDSVSIPAGLYRVGLP
                     ATPPLSRTPPLPSFLLEKGLVAGPGETAAPLAAPAASAEYRELGPEAAKAAALLLEGA
                     PRLEAQRPPVTILPEERKSPSGGEDYALALIAKEKLLPGDAAGAAEQLRKYLSLNRGP
                     RAAARARYYLGEALAVEGAYREAFFEFLRARELFAAETAPWIDYLIAVLRRG"
     gene            4134..4424
                     /gene="cas2"
                     /locus_tag="PLG14_09230"
     CDS             4134..4424
                     /gene="cas2"
                     /locus_tag="PLG14_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOX49352.1"
                     /translation="MFVAIVCDMGNEDSRAALYNLLPQYGFERVQKACFESASVTDRQ
                     LAALKRDIDRITDSYDTIRLYQYPVDGTLALTVLKDNKWRRIVVRDPSRPRE"
     gene            4501..5486
                     /gene="prfB"
                     /locus_tag="PLG14_09235"
                     /pseudo
     CDS             4501..5486
                     /gene="prfB"
                     /locus_tag="PLG14_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758144.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          900 a         1951 c         1872 g          763 t
ORIGIN      
        1 cgacttcctc tgccaggaga tgaaccgcga ggtcaacacc atcggctcca agaacatcct
       61 cctccccgtc gccgaggccg tcgtcgagct caaggacgcc ctggagaacg tgcgggagca
      121 gctccgcaac atcgaatagg aagggacggg acatgggagc gagcatcgtc gcgatatcgg
      181 gaaagtcggg ctgcggcaac agcaccgtga cgaggctcct cgcggagcgc ctcggattca
      241 aggtcgtcaa ctacaccttc aggaacatgg ccgccgaccg cggcctcacc ctcgcgcggg
      301 tcctggagct cgccggccag gacgactcct gggacaggga cctcgacgcc caccaggtcg
      361 agctcgcccg cgccggggac tgcgtcatcg gctcgcgcct cgcgatgtgg ctcctcccgg
      421 aggcggccct gcgggtctac ctctgggcga gccccgagat ccgcgcggcg cgcatccaca
      481 cccgggaggg cggcagcctg gacgaggtca tcgccttcac gcggaagcgg gacgaggcgg
      541 accgggcccg ctacaggcgc atctacggcc tggacaacga cgactacagc gaggcggacc
      601 tcaccgtcaa cacggagcga ttcgggcccg cggagatcgc ctcgatcctc gcggaagccc
      661 tggcgcgcaa gctcgcgccc aaggcatagg aaggagacag catggagcgt cgcgaaccgg
      721 tagcgctggt caccggagcc tcgagcggca taggcaacgc ctgcgccacc tatctcgcca
      781 agcggggcta caaggtctac ggcacctcga ggaacccagc ctcctacgcc aagaaggccg
      841 acgagtactt cgagctcgtc cagatggacg tcacggacga ggcctccgtc aggaaggcgg
      901 tggacgagat cctcgccaag gagggcagga tcgacgccct cctgtgcaac gccggcatgg
      961 gcatctcggg ctccgtggag gactcgagcc tcgaggagat ccgcctccag ctcgagacca
     1021 acttcctcgg ggtggtgcgc tgcgtgaagg ccgtccttcc cgggatgcgc caggccaagg
     1081 cgggccgcat cctcgtcctc tcctcgatag cgggcgtcat cggcatgccc ttccaggcct
     1141 tctactccgc gagcaagttc gcgctggagg gcttcgtgga ggccctgcgc tacgaggcct
     1201 atcccttcgg cgtccaggcg gccctcatcg agccgggcga cttccggacg ggcttcaccg
     1261 acgcgcgccg ccccgtcaag gcggccgagg gcagccacta caagaagctc tacgacgcgg
     1321 ccatgggcgt ccaggtccgc gacgagcggg ccggccacga ccccctggag gtggcgaagc
     1381 tcgccgtccg cctcctccag aagcgcaggc tcaagatccg ctactcggtc ggcccggcct
     1441 tcgagcgctt cagcgtggtc ctgaagcgcc gcattcccga cctcctcttc gagtacggct
     1501 accgcaagta ctacaagctc ccctgaggcc gccttaagat gtccatacgg aagacaagct
     1561 tcgggacgac ggcagagggc ttcgaggcga gcctctacac gctgtcctca ggcggcctcg
     1621 aggccggcct ctccgacttc ggcgcgaccc tcgtcttcct ggaggccccc gcgggccgcg
     1681 gggagacggt cgacctcgtc ctcggcctcg aatcggccgc cgcctacgag gcctccgacg
     1741 cctacctggg cgcgacggcc gggaggttcg ccaaccgcat cgcgaagggg cgcttcagcc
     1801 tggacgggaa ggactacggc ctggcggcca acaacgggcc caaccacctg cacggcggcc
     1861 ttcgcggctt cgacaagcgg ccctggaggg tcgaggcctc ggagctcggc ggctcgcccg
     1921 cgctccgctt ctcccgcctg agccccgacg gcgaggaggg ctaccccggc gcgctctcgg
     1981 tctccgccac ctatgtcctc gagcccgagg gcgtgctccg cgtcctcttc gaggccggca
     2041 cggacgcggc caccctcgtc tccctgacgc agcacgccta cttcaacctg cgcggcgccg
     2101 gctccggcga cattctgggc cacgacctcg agctccgcgc ctcgcgctac ctgcccatcg
     2161 acaaggacct gattccgacg ggagagctcg cgccggtaga aggcggcccc ttcgacttcc
     2221 gcgcggcgaa gcccctgggg cgcgacatcg aggccctggg cgacgcggcg ggcggctacg
     2281 accactgctt cgtcctggac cgcgacggcg acggcctctt cgagttcgcg gagctccgcg
     2341 agcccctttc ggggcggcgc atgaccgcgg cgacgaccct tcccggtgtc cagctctata
     2401 cggggaattt cctgtcgggg gcgaagggga ggaagggcca ggtctacgcg aggcggggcg
     2461 gagtctgcct ggaggcggag ctcttccccg attcgcccaa ccgccccgcc ttcccgagtc
     2521 ccgtcctgcg cccgggcgag acctggcgcc acgagaccct ctaccgcttc ttcctctagc
     2581 ggccttcgcc ccggttccgc tccaggccga gcctggcccg gcctagccgc gcctcagcac
     2641 ggcgatcagg tagtcgatcc agggcgcggt ctcggcggcg aagagctccc tggcccgcag
     2701 gaactcgaag aaggcctcgc gataggcgcc ctcgacggcg agggcctcgc ccaggtagta
     2761 gcgggccctg gccgccgccc tggggccgcg gttcaaggac aggtacttcc ggagctgttc
     2821 ggcggccccc gcggcgtcgc ccgggaggag cttctccttc gcgatgaggg ccagggcgta
     2881 gtcctcgccg cccgaggggc tcttcctctc ctcgggcagg atggtcacgg ggggcctctg
     2941 ggcctcgagg cgcggcgccc cctccaggag tagggccgcg gccttggcgg cctcggggcc
     3001 aagctcgcgg tattcggcgc tggcggcggg ggcggccagg ggagcggccg tctctccggg
     3061 cccggcgaca agtcccttct cgaggaggaa ggaggggagg ggcggcgtcc tggacagggg
     3121 cggcgtcgcg gggaggccga cccggtagag gccggcgggg atgctgacgg agtcgcgggt
     3181 cgcgttcctg ccgggctcga cggaaagccg gccggcgcgg agctcctcct cggggaggat
     3241 ggcgtaccag tagtcgacgc cggggacggg gtagtcggcg atggcgcccg acttgtcctc
     3301 gaaggccgcg acgagcaccg cgtcgaggag gctcgccgag ccgaggatgg gctggctgct
     3361 ccggtagagg acgagtctct ggcccggaat cctcgacctg tagctcacga cgacggcgtc
     3421 cttggcctgg gccgccgcca cgccttcgag gctgggcggc tccgctgcgg tcgccgccgc
     3481 gggcgtggcg ggagcggcgg gagcgggagc ggcgggaagg gactcggaag gaggcagggc
     3541 tatcgcggcc agggtggtgt tccgggcggg gatgaagacc ttgtagggct cgccctcgct
     3601 cccgacggcc aggacgaggt agtagtaggc cgcgccgccc gaggggcggt cgacgtaggt
     3661 gccgatgccc gactcgacct ccccgacctt ctcggctccg gcgaaggccg ataggccgtc
     3721 gatgggcgcc agggagcggt agaccgcgta gcgacccttc acgtcggcgg aatcctccca
     3781 ggagaggacg acctggccct ccctgagggc ggccctgagc ttggagggga agggcgcgaa
     3841 gacggcctcg gagggcgcct cctcggccgt ctcgaccccg gccggagccg gctcctgggc
     3901 gccaagggtg gaaaccgcga aaaggccggc tagggcgagg cagcagtgat accgcataat
     3961 atatatgtat cggatcaaag ccggctctac cagaggcccg gcgcgcatct ttgccgtccc
     4021 tgcggcgccg ccctgatgcc tttggcgctg tccgaagacc ccaagcgccg gccggaggcc
     4081 catggcgcgg gccgcgaatt gacgggacag gccgtttccg ctaccatgag cccatgttcg
     4141 tcgccatagt ctgcgacatg gggaacgagg attcccgcgc cgccctctac aacctccttc
     4201 cccagtacgg cttcgagcgg gtgcagaagg cctgcttcga gagcgcctcc gtgaccgacc
     4261 gccagctcgc ggcgctcaag cgcgacatcg accgcatcac cgactcttac gacacgattc
     4321 gcctctacca gtatcccgtc gacggcaccc tcgccctcac cgtcctcaag gacaacaagt
     4381 ggcgcaggat agtcgtccgg gatccctcgc gccccaggga atgagggcca aggcgccggc
     4441 cccgcgccct cgcgggccgc acacgaagac aggatacgac gaccgcccca ggaggaaccc
     4501 atgatcgaag agctcgccaa tcccatcgcc tcgctccgcg gggaaatcct ggacatctgg
     4561 gggcgtcttt gacgcccctt ccatagagaa gcggatagcc gagaaggagg cccagagcgg
     4621 agagcctggc ttctggaacg acggggccaa ggccggccgc gtcatgaccg agatcaagca
     4681 gtggaaggac cgcctggaca cctggacgcg cctcaaggcc gacgtcgagg ccctgcgcga
     4741 ggtctacgcg atggcccgcg acgagggcgg cgactccatg gagggcgacc tccgcgcctc
     4801 cctggacgac atcaaggccc gcttcgacaa ggccatcgtc ttcgagctcc tctccggcga
     4861 ggccgacccc tccagcgcct tcctcaccat ccacgccggg cagggcggca cggaggcctg
     4921 cgactgggcc tcgatgctcc tgcgcatgta cacccgctgg tgcgagcgcc gcggctacaa
     4981 gtacgaggtg atcgacctcc aggaggccga gggcggcgtc aagagcgcga cgatccagat
     5041 cgacgcgccc tacgcctacg gctacctcaa aggcgagtcg ggagtccacc ggctggtgcg
     5101 catctcgccc ttcgacgcca acgcgaggcg ccacacctcc ttcgcctccg tctacatctt
     5161 ccccgtcctg gacgactcca tcgacgtgga gatcaggccg gaggacatcc gcatcgacac
     5221 ctaccgctcg ggcggcgccg gcggccagaa ggtcaacaag accgactcgg ccgtccgcat
     5281 cacccacctg gagacgggca tcgtcgtcac ctgccagaac gagcggagcc agtacaagaa
     5341 caaggacgtc gcgatgtccg tgctcaagtc ccggctctac gaggactacc gcctggagaa
     5401 ggagaaggag aactccaagt tcgcctccga aaagaaggag atcgcctggg gctcccagat
     5461 ccgctcctac gtcttccagc cctaca
//
LOCUS       DAOOSJ010000183         5454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9341_length_5454_cov_4.779589, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000183 DAOOSJ010000000
VERSION     DAOOSJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9341_length_5454_cov_4.779589"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLG14_09240"
     CDS             complement(<1..229)
                     /locus_tag="PLG14_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49353.1"
                     /translation="MKKIAAILVILVALFAVVGCASKPAAPAGGVQPPAVTVKTYPPQ
                     ILEHKGTAFGRDYPKWMDAALDGPKAVEKLGD"
     gene            complement(339..1424)
                     /locus_tag="PLG14_09245"
     CDS             complement(339..1424)
                     /locus_tag="PLG14_09245"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOX49354.1"
                     /translation="MPKTKLPFIALAMASLLSACATTAPASAPAAGPAAPQASAPAAG
                     DSAAKDRAKALVQGGYDAGAAIPAPAQPAAGAAAGTAAAQPASASADQPPAPGAPLTP
                     EEQAYLQNYLARLNYMVYYNEDAKVEPRLAKIAVSQANRYLIEQSGLSVIDFDQIEKN
                     KKDQQAAYQSETGGSIDLIQYLAQKFNADVYVELDFAVSTETRDGKYYASAQGSMKLF
                     DTSTASLLGSVAFMSQPAFSPSSMDAAVANAIAASVWTAMPKMTGQAKELIKNSLGRG
                     VRYEVVVQNTPDSRQVSNLRRALAKKVREVEQVSYSPNETKLYLYTFQGRDKVEDAVY
                     DAAAAAGLNDLNLVYMRGKSFTFNSGL"
     gene            complement(1731..3743)
                     /locus_tag="PLG14_09250"
     CDS             complement(1731..3743)
                     /locus_tag="PLG14_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49355.1"
                     /translation="MRAPFPASALAALALAALLLAGCDASSSLFSEAERSAIYDLSLS
                     FRGGPVAEGSALEGGAELEVELGLMRGAEAPALLSIELQDAKGGVLSSRRYETEAAAK
                     AAEPGSQAAGSSAGTAADSAAAAEAPLRVKSLSGSLPSLRLPESLPPGAYRLETRILG
                     REGALLDRSAILVFVGLPGQAAPALSLYPPKPRPGSAVLLSASLSGVEGDPWLRWSAD
                     GRLLAEGPRSKGLDKTVWRAPGLASAYAIRVDVYPAPPEGRGYEVGSPWRQDMKAIVS
                     GPPLADEYSDPTSFLSRFAFDGSFEESGSRLIEERPLAFGSPRLEVYSGGFGYRLGPA
                     AGLSAPGAVPPSEEGRPRAFSLLWRLGLGAAALEGSSTLARFLDVEGREVLVVGVEEG
                     RPFLSLGREPGAARSRAGFLLSPGTVDLALSLEAEGDALLPVWTLAGEASPSPSLPLR
                     SFTPPAELSLGGPGSLEAVYDEFGLRDDSASGPPALYRAAARRAYGESLILAEGFEAF
                     ALPSGLSVAGLAGLGPRSLSLGQDASLQVQEPLPLERALAAELRFEGRAEGLVVELSD
                     GEGAVLLSMAGSGEVLGPDGAILGRLGREAGRLAFALRAQGGFLELSPPRGLPVARAA
                     LPGSLASVSLSLRNSGERPVVLRRLLVRAAPELLAQGPRPSLARLP"
     gene            complement(3845..>5454)
                     /locus_tag="PLG14_09255"
     CDS             complement(3845..>5454)
                     /locus_tag="PLG14_09255"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOX49356.1"
                     /translation="LVVFPELALCGYPPMDLLDQDSFVEASLASLRRLQRELPPGLAV
                     AVGYVDRNRGGSGRPLVNAVAVIKDGEVLFSQAKTLLPTYDVFDEARYFEPARERRVF
                     ELYGKRIGVAICEDIWWEEPPLPGFRYAVDPVKELLDAGAELLVVPSSSPFHKGKLAT
                     RLRLAGDAAREGGIPVLYCNAVGGNDSLVFDGRSFALDSRGDPVLVAGFEEGLFLVDS
                     GALELVPGPKAGSRPEGEGAAAALGEADPWEEVEAALVLGTRDYLKKCGFSKACLGLS
                     GGVDSALVAVIAARALGPENVACLSMPSRFSSAGSVDDSVELCRRVGLRMETLPIEGP
                     FAAYLSLLEPKFEGRPMDLTEENLQARIRGAILMAWSNKFGSLLLTTGNKSELAAGYC
                     TLYGDMCGAFNPIGDLLKTEVYALCRSINARAEAAGLVAPIPAPIIDKAPSAELRPNQ
                     KDQDSLPPYEELDAILELYIVRNLSAAEIVGLGHEPALVRRIVTLVARSEYKRRQSAP
                     VVKVSPRAFGTGRRIPIARAVHEARLP"
BASE COUNT          805 a         1784 c         2166 g          699 t
ORIGIN      
        1 agtcgccgag cttctccacg gccttggggc cgtccagggc ggcgtccatc cacttgggat
       61 agtcgcggcc gaaggcggtg cccttgtgct cgaggatctg gggcggatag gtcttgaccg
      121 tcacggcggg cggctggacg ccgccggcgg gggcggcggg cttgctcgcg cagccgacga
      181 cggcgaaaag cgcgacgagg atgacaagga tcgcggcgat cttcttcacg ttaacctcct
      241 ggaaaactgt tttctacggg ccccttgggc cctatgttcg cgcgggcgcc cgtccctcgc
      301 ggcggctctc gccgccgggc cggaccgccc gcggcggttc acaatccgga attgaaggtg
      361 aaggacttcc ccctcatgta gaccaggttc aggtcgttga ggccggcggc ggcggccgcg
      421 tcgtagaccg cgtcctccac cttgtccctg ccctggaagg tgtagaggta gagcttggtc
      481 tcgttcgggg agtaggagac ctgctcgacc tcgcggacct tcttcgcgag ggccctgcgg
      541 agattgctca cctggcggga gtcgggcgtg ttctggacga cgacctcgta gcggacgccg
      601 cggcccaggg agttcttgat gagctccttg gcctggcccg tcatcttggg catggcggtc
      661 cagacggagg cggctatcgc gttcgcgacg gcggcgtcca tggagctcgg actgaaggcg
      721 ggctggctca tgaaggccac cgagccgagg agggaggcgg tggaggtgtc gaagagcttc
      781 atggagccct gggcggaggc gtagtacttg ccgtcgcggg tctccgtgga gacggcgaag
      841 tcgagctcga cgtagacgtc ggcgttgaac ttctgggcca ggtactggat gaggtcgatg
      901 gagccgccgg tctccgactg gtaggcggcc tgctggtcct tcttgttctt ctcgatctgg
      961 tcgaagtcga tcacggagag gcccgactgc tcgatcaggt agcggttggc ctgggagacg
     1021 gcgatcttcg cgaggcgcgg ctccaccttc gcgtcctcgt tgtagtagac catgtagttg
     1081 aggcgggcca ggtagttctg gaggtaggcc tgctcctcgg gggtgagggg ggcgccgggg
     1141 gccggcggtt ggtcggcgga ggcggaagcc ggctgggcgg cggcggttcc ggcggcagcg
     1201 cccgcggccg gctgggcggg cgcggggatg gcggcgccgg cgtcgtagcc gccctggacc
     1261 agggccttgg ccctgtcctt ggccgcggag tcgccggcgg cgggcgcgga ggcctgcgga
     1321 gcggccggac ccgcggcggg agccgaggcg ggagccgtcg tcgcgcaggc tgagaggagg
     1381 gaggccatcg cgagggcgat gaacggcagc ttcgttttcg gcatgggtcg ctccttcgtt
     1441 accgggtgtt aaatctagcg cgccgcggcg aaaaaaacaa agcggccgcc ttgtctcgga
     1501 ccgacggccg cctcgcggga cgcgcgaggc gaaggggccg ctggccgcgc atgatcagtc
     1561 tatagccggg aaggggaaga cgcaagcgct cgggccgttt ccagggctgc ccgacgcagc
     1621 caagccgtcc tcaaagcccg ccgtagccaa gccgccctca cggcccgtcg cagccaggcc
     1681 tccttcacgg cccgccgcag ccggccgccc tcagggaagc ctggctgtct tcagggtagc
     1741 ctggccagcg aaggcctcgg gccctgggcc aggagctcgg gcgcggcccg gacgaggagg
     1801 cgcctcagga cgacgggcct ttccccggag ttcctcaggg agagggagac cgaggcgagg
     1861 ctgcccggca gggcggcgcg cgcgacggga aggccgcggg gaggcgaaag ttccaggaag
     1921 ccgccctggg cgcggagcgc gaaggcgagc cgccccgcct cccttccgag ccggcccagg
     1981 atcgcgccgt cgggccccag gacctcgccc gagcctgcca tcgagaggag gacggcgcct
     2041 tccccgtccg agagctcgac gaccagcccc tcggcccggc cctcgaagcg gagctcggcg
     2101 gccagggcac gctccagggg gaggggctcc tggacctgga ggctggcgtc ctggcccagg
     2161 gagagggagc gcggcccaag cccggccagg cccgcgacgg acaggcccga gggcagggcg
     2221 aaggcctcga agccctcggc caggatgagg ctctcgccgt aggcccgcct agcggccgcc
     2281 cggtagaggg cgggcggccc cgaggccgag tcgtcgcgca ggccgaactc gtcgtagacg
     2341 gcctcgagcg agccggggcc gcccaggctg agctccgcgg ggggcgtgaa ggagcggagg
     2401 ggcaggctcg gcgagggcga agcctcgcca gccagggtcc agaccgggag gagggcgtcc
     2461 ccctcggcct cgagggagag ggcaaggtcg accgtgccgg gggagaggag gaagccggcc
     2521 ctgcttcgcg ccgcgccggg ctcccggccc agggagagga agggccgccc ctcttcgacg
     2581 ccgaccacga ggacctcgcg gccctcgacg tcgaggaaac gggcgagggt ggaggagccc
     2641 tccagggcgg cggcgccaag gccgaggcgc cagaggaggc tgaaggcccg gggcctcccc
     2701 tcctccgagg gcgggacggc gccgggagcc gaaaggccgg cggcggggcc gagcctgtag
     2761 ccgaagccgc cagagtagac ctcgaggcgg ggggagccga aggcgagggg gcgctcctcg
     2821 atgaggcggg aacccgactc ctcaaaggag ccgtcgaagg cgaagcgcga gaggaagctc
     2881 gtcgggtcgg agtactcgtc ggcgaggggc ggccccgaga cgatggcctt catgtcctgg
     2941 cgccagggcg aaccgacctc gtagcccctg ccttcgggag gggcgggata gacgtcgacg
     3001 cggatcgcgt aggcggaggc gaggccgggg gcccgccaga ccgtcttgtc gaggcccttg
     3061 gagcgggggc cctcggccag gaggcggccg tcggcgctcc agcggagcca gggatcgccc
     3121 tcgaccccgg agagggaggc ggagaggagg acggccgagc caggccgggg cttgggcgga
     3181 tagagggaga gggccggcgc ggcctggccg ggaaggccga cgaagacgag gatcgcgctc
     3241 cggtccagaa gggctccctc gcggcccagg atgcgggtct ccaggcggta ggcgcccggc
     3301 ggaaggcttt ccggaaggcg gagcgacggc aggctgccgg agaggctctt cacccggagc
     3361 ggcgcctcgg ccgccgcggc cgagtccgct gcggtcccgg cgctggagcc tgccgcctgg
     3421 gagcccggct ccgcggcctt cgcggccgcc tccgtctcgt agcggcggga ggagaggacg
     3481 ccgcccttcg cgtcctggag ctcgatggag aggagggccg gggcctcggc gccgcgcatg
     3541 aggccgagct cgacctcgag ctcggcgccg ccctcgagag ccgagccttc ggccacgggg
     3601 ccgccccgga aggagaggga gaggtcgtag atggcgctcc gctcagcctc cgagaacagg
     3661 ctcgaggagg cgtcgcagcc cgcgagaagc agggccgcca aggccaaggc cgcgagggcg
     3721 gaggccggga agggggccct caaggccgcc cttccggccg gggccgcgcc tcttcggaga
     3781 aaatcacgcc gcatgccgct ccatcgagtc aaatatagcc cgcttccccg cccctttcca
     3841 aagcctaggg aaggcgggcc tcgtggaccg cccgcgctat ggggatgcgc cgccccgtcc
     3901 cgaaggcccg cggggagacc ttgacgacgg gcgcgctctg cctccgcttg tactccgagc
     3961 gggcgacgag ggtgacgatc cgccgcacga gggcgggctc gtggcccagg ccgacgatct
     4021 ccgccgcgga gaggttcctc acgatgtaga gctcgagtat ggcgtccagc tcctcgtagg
     4081 gcggcagcga gtcctggtcc ttctggttcg gcctgagctc ggccgagggc gccttgtcga
     4141 tgatcggcgc cgggatgggc gcgacgaggc cggcggcctc cgcgcgggcg ttgatcgagc
     4201 ggcagagggc gtagacctcc gtcttgagga ggtcgccgat cgggttgaag gccccgcaca
     4261 tgtcgccgta gagggtgcag tagccggccg cgagctcgct cttgttcccc gtcgtgagga
     4321 ggagggagcc gaacttgttg gaccaggcca tgaggatggc gccgcggatc cgcgcctgga
     4381 ggttctcctc cgtgaggtcc atgggccgcc cctcgaactt gggctcgagg agggagaggt
     4441 aggcggcgaa gggcccctct atgggcaggg tctccatgcg gaggcccacg cggcggcaga
     4501 gctcgacgga gtcgtccacc gagcccgcgc tcgagaagcg gctcggcatg gagaggcagg
     4561 cgacgttctc cggccccagg gcccgggccg cgatcaccgc gaccagggcc gagtcgaccc
     4621 cgcccgagag gccgaggcag gccttggaga agccgcactt cttgaggtag tccctggtcc
     4681 cgaggacgag ggcggcctcg acctcctccc agggatcggc ctcgcccagg gccgcggccg
     4741 cgccttcgcc ctccggccgg ctgccggcct tcgggccggg gacgagctcc agggcgcctg
     4801 agtcgaccag aaagaggccc tcctcgaagc ccgcgaccag gacggggtcg ccccgcgagt
     4861 ccagggcgaa ggagcggccg tcgaagacca gggaatcgtt ccccccgacc gcgttgcagt
     4921 agaggacggg gatgccgccc tcgcgagccg cgtcccccgc gaggcggagc cgcgtcgcga
     4981 gcttgccctt gtggaagggc gaggaagagg gcacgacgag gagctcggcc ccggcgtcca
     5041 ggagctcctt cacgggatcc acggcgtagc ggaagccggg caggggcggc tcctcccacc
     5101 agatgtcctc gcagatcgcg acgccgatcc gcttcccgta gagctcgaag acccgccttt
     5161 cgcgcgcggg ctcgaagtag cgggcctcgt cgaagacgtc gtaggtgggt aggagggtct
     5221 tggcctggga gaagaggacc tcgccgtcct tgatgaccgc gacggcgttg acgaggggcc
     5281 tgccgctccc gccgcggttg cggtccacgt agcccacggc gacggccagg cccgggggca
     5341 gctcgcgctg gaggcggcgc agggaggcaa gggaggcctc gacgaaggag tcctggtcca
     5401 ggaggtccat gggcgggtag ccgcagaggg cgagctcggg gaagacgacg aggt
//
LOCUS       DAOOSJ010000184         5449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9354_length_5449_cov_5.641824, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000184 DAOOSJ010000000
VERSION     DAOOSJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_5449_cov_5.641824"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..322)
                     /locus_tag="PLG14_09260"
     tRNA            complement(249..322)
                     /locus_tag="PLG14_09260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Ile,seq:cat)
     gene            450..>1792
                     /locus_tag="PLG14_09265"
     CDS             450..>1792
                     /locus_tag="PLG14_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX49357.1"
                     /translation="MRKIPVVSSDEKLNEVVRLECARFGSEFAPVFFKDRDEVIEFLK
                     YELPEMKIFYFSDKEADCRGILKEIQEDPWLHYGGIVAIHDDQDEKEALEAMRDANII
                     AVLRAREFMAGLPRLLKILRQNKQILFQRGIQQHLLKTISGAFVMDNDPLDITTYANL
                     VTNYLYNANLIGRDEKEKLHVALLELLINAIEHGNCRIGFDEKTAWLERNGDIMELIR
                     EKNRDPAVRARKVYFSYTITPERSRFDIRDEGEGFDWRARLSSGDGVVEPGLHGMGMR
                     MANLYVRELAYNDKGNAVSFEVEHRRGESNSIPKIFEQAAEERFADGEYVCREGEESD
                     FLYYIVSGTLYVYSKGKLVSSLSPDDMFMGEMSFLLSNRRSATVVAKGPSVLIKISKQ
                     DFVNLIKENPHYGIFLARLLAQRLARLNARTARLNTEYLKLKALCGPDGQGCGPQA"
     assembly_gap    1793..1892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2024..2521)
                     /locus_tag="PLG14_09270"
     CDS             complement(2024..2521)
                     /locus_tag="PLG14_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOX49358.1"
                     /translation="MTKAEKLELIDQFEGAFGQVEGLVAGLPDAALRYKPALPEAWTI
                     EEHLVHLLDAEAASWFRIRAAVAEPGKAIVLWEEDSWRERLAYGAVGGKAALALAKGL
                     RSALAASLRALADEVWEGFWIEHPSRGRVLLDELLETYRDHLAFHAPFVKRNREAWEA
                     AGRMA"
     gene            complement(2648..3886)
                     /locus_tag="PLG14_09275"
     CDS             complement(2648..3886)
                     /locus_tag="PLG14_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOX49359.1"
                     /translation="MNQRPARARPRVLALLCLLSSLSAAAAHGAAGSAASGAASGAKA
                     FPPDGKAPAQPVLPRAAFSPAEAALRRAAVLAHPERYVTIGSAPAEFRTLKEALAAPD
                     DGRRTFVLTDPVHREEGTRIGRDLTLCGFGAGESVLEAAASAAEAKGRVLEVERGASV
                     LLAGLTVRNGRIMDVPRRAGGIANSGTLSMEDCAVSGNVATYGVGVWTEGRLRMLRCA
                     VTGNVGRPRPQPDQYKGVDDGGAGAGLRIEKGGFALVEDCLIAWNEAPTAGGGVQLCC
                     EASARLVDTTIFGNRAGVRGGGVNQSGGSLTLEACSIAGNRAAAKGQAFFHRGRLKMS
                     GSLIAAEAGLAYFLSTDKGGDVGRGILELNEGNFCESGELPGAATGKLGVLLLADSGG
                     ALPVLALPEGSPARGYGARP"
     gene            3982..>5449
                     /locus_tag="PLG14_09280"
     CDS             3982..>5449
                     /locus_tag="PLG14_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOX49360.1"
                     /translation="MHIAYLDGHRLSRALVAGARRLQERAGRLNAINVFPVPDGDTGT
                     NMAGTARAMALRLEALPAGSLGFGAAMRAAADSALRGARGNSGAILAQFLHGLAEELE
                     GEARIGTRRFAEAARSAARRARAALSKPREGTIVTVLTDWAEAAHRVAAKTDDFLVLL
                     KAALEEARASLARTPDLLPELKKAGVVDAGAEGFVHILEGIWAYVSGGRLRDRARAGR
                     SGAARHAERPADEALPGAGSERAHVADGLAESLSAAEDSVLPGLSDAAADGGHEESAF
                     RYCTECLVTGEGLDLEALRAGLVELGDSLVVAGSSTLARVHLHTDRPQAAFRLLAEAG
                     SGASAVEGQKVDDMELQRRLSAAPRRPAAVLTDTACDLPDALGLELLADRAAVLVEIG
                     GKAYLDRDGLSSDEFFGMLRADPAAPLRSSQPSHAAFSRKLDLLLERADEVVYVGLSA
                     ALSGTLEGGRRAAGEGPRAGRVRVVDTRSISVGAALVVR"
BASE COUNT          781 a         2050 c         1755 g          763 t
ORIGIN      
        1 gagtttgggc cctgctggac ttgccctccg ctcggcgcct tccaggcgcc ttcgctccca
       61 ttgcccctca tcgattcggg gcaaagcctc gcggggctcg cgcgggcgtc cgtgcccgcg
      121 cgtacgccgc gcgccctcgc cggctcgggc gcgcggcgaa gcgcccggct cggttcaagt
      181 ccagcagggc ccgcttggct tcgccgcggc gcttctatgg ggtggtattc gcgtttgaaa
      241 ggggagtttg ggccctgctg gacttgaacc agcgacctac ggattatgag tccgcagctc
      301 taaccaactg agctaagggc ccggaaaagg gcctttccat aaagacatag gggtcgaaaa
      361 aagtcaatcg gaggggaatt cgggccgatt ttctatagcg gagcgccccg gccaggggcg
      421 ccgtgccgca agaaagcagg ggtgcccgca tgcgaaagat acccgtcgtc tcgagcgacg
      481 agaagctcaa cgaagtcgtc aggctcgagt gcgcgcggtt cgggagcgaa ttcgcgcccg
      541 tcttcttcaa ggaccgcgac gaggtcatcg agttcctgaa gtacgagctg ccggagatga
      601 agatcttcta cttctccgac aaggaggcgg actgccgcgg gatcctcaag gagatccagg
      661 aggacccctg gctccactac ggcggcatcg tggccatcca cgacgaccag gacgagaagg
      721 aggccctgga ggcgatgcgg gacgcaaaca tcatcgcggt cctgcgcgcc cgggagttca
      781 tggccggcct cccgcgactc ctcaagatcc tcaggcagaa caagcagatc ctcttccagc
      841 gcgggatcca gcagcacctc ctcaagacca tctccggcgc cttcgtgatg gacaacgacc
      901 ccctggacat cacgacctac gccaacctcg tgacgaacta cctctacaac gcgaacctca
      961 tcggccgcga cgagaaggag aagctccacg tcgccctcct cgagctcctc atcaacgcga
     1021 tcgagcacgg caactgcagg atcggcttcg acgagaagac ggcctggctc gagaggaacg
     1081 gcgacatcat ggagctcatc cgcgagaaga accgcgaccc cgcggtgcgc gcgcggaagg
     1141 tctacttctc ctacacgatc acgccggaga ggtcgcgctt cgacatccgc gacgagggcg
     1201 agggcttcga ctggcgcgcc cgcctctcct ccggggacgg cgtcgtcgag ccgggcctcc
     1261 acggcatggg catgcggatg gcgaacctct acgtgcgcga gctcgcctac aacgacaagg
     1321 gcaacgcggt gagcttcgag gtcgagcacc gccgcggcga gtccaactcg atcccgaaga
     1381 tcttcgagca ggccgcggag gagcgcttcg ctgacggcga gtacgtctgc cgcgagggag
     1441 aggagtcgga cttcctctac tacatcgtct cgggcaccct ctacgtctac tcgaagggaa
     1501 agctcgtctc ctccctctcg cccgacgaca tgttcatggg ggagatgtcc ttcctcctct
     1561 ccaaccgccg ctcggccacc gtcgtcgcga agggcccctc cgtcctcatc aagatctcca
     1621 agcaggactt cgtgaacctc atcaaggaga atccccacta cgggatcttc ctcgcccgcc
     1681 tcctcgcgca gcgcctcgcg cgcctcaacg cgcgcacggc ccgcctcaac acggagtacc
     1741 tgaagctcaa ggccctctgc ggtcccgacg gccagggctg cggtccccag gcnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacctgaag ctcaaggccc tctgcggtcc
     1921 cgacggccag ggctgcggtc cccaggccta ggccacggga ccgcgacgca agcccggggc
     1981 ctgagccaag cctcgatacc ctggcctggg ctcgccccgc agcctaagcc atcctgccgg
     2041 cggcctccca ggcctcgcgg ttccgcttga cgaagggcgc gtggaaggcg aggtggtcgc
     2101 ggtaggtctc caggagctcg tccaggagga ccctcccgcg gctcgggtgc tcgatccaga
     2161 agccttccca gacctcgtcg gcgagggccc ttaaggaagc cgccagggcc gagcggaggc
     2221 ctttcgccag ggccagggcc gccttgccgc caactgcccc gtaggcgagg cgctcgcgcc
     2281 aggagtcctc ctcccagagg acgatggcct tccccggctc tgcgaccgcc gcgcggatcc
     2341 tgaaccagga ggccgcctcc gcgtccagga gatggaccag gtgctcctct atcgtccagg
     2401 cctcgggcag ggccggcttg tagcgcaggg ccgcgtcggg caggccggcc acgaggccct
     2461 caacctggcc gaaggctccc tcgaattggt cgatcagctc gagcttctcc gccttcgtca
     2521 tgctccctcc tctcgcgcgc cgcgcgccgc tccgtgccgc tccgcataac tccgcgccgc
     2581 acggcgccgc gattagagtc tagcctccgg ccggcgcccg cgccagggcg cgttccgcgc
     2641 gccgctttca gggcctcgcg ccgtagcccc gcgccggcga gccctcgggc agagccagga
     2701 ccggcagggc gccgcccgag tcggccagga ggaggacgcc gagcttgccc gtcgccgctc
     2761 ccggcagctc ccccgactcg cagaagttgc cctcgttgag ctccaggatc ccgcgcccga
     2821 cgtccccgcc cttgtcggtg gagaggaagt aggcgaggcc cgcctccgcc gcgatgaggc
     2881 tgccgctcat cttgagcctc ccgcggtgga agaaggcctg gcccttcgcc gcggccctgt
     2941 tgcccgcgat cgagcaggcc tcgagcgtca ggctgccgcc cgactggttc acgccgcctc
     3001 cgcggacgcc ggcgcggttc ccgaagatgg tcgtatccac caggcgggcg gatgcctcgc
     3061 agcagagctg gacgccgccg cccgccgtcg gcgcctcgtt ccaggcgatg aggcagtcct
     3121 ccacgagggc gaagccgccc ttctcgatcc tgaggcccgc gcccgcgccg ccgtcgtcca
     3181 cgcccttgta ctggtcgggc tggggccggg gccggccgac gttgcccgtg acggcgcagc
     3241 gcagcatcct gaggcggccc tccgtccaga ccccgacgcc gtaggtcgcg acgttgcccg
     3301 acaccgcgca gtcctccatg gagagcgtgc ccgagttcgc tatcccgccc gcgcggcggg
     3361 gcacgtccat gatccggccg ttcctcaccg tgaggcccgc aagcaggacg ctcgcgcccc
     3421 gctcgacctc caggaccctc cccttcgcct ccgccgcgct cgcggccgcc tccaggacgc
     3481 tctcccccgc gccgaagccg cagagggtca ggtcgcggcc tatcctcgtg ccctcctcgc
     3541 ggtggacggg atccgtgagg acgaaggtcc tcctcccgtc gtcgggcgcg gccagggcct
     3601 ccttgagggt cctgaactcg gctggcgccg accctatcgt cacgtagcgc tcgggatggg
     3661 ccaggacggc ggcccgacgc agggccgcct cggccgggga gaaggcggcc ctgggcagga
     3721 cgggctgggc gggggctttc ccgtccgggg ggaaggcctt ggcgccgctt gcggcaccgc
     3781 ttgccgcaga tcccgcggcg ccgtgggcgg cggccgcgga tagcgaggaa aggaggcaga
     3841 ggagggcgag gactcgcggc ctcgcgcggg ctggtcgctg attcatggcc cattgtcccc
     3901 cgcccgtcct ggcggcgcaa tccttgattc gggggaggcg tacttgatcg cggccacaat
     3961 tacagttatt gtaatattgt catgcatata gcctacctcg acggacaccg tctttcccgc
     4021 gccctggtcg ccggagcccg ccgcctccag gaacgcgccg gccgcctcaa cgccatcaac
     4081 gtcttccccg tccccgacgg ggacacgggc accaacatgg ccggcacggc ccgggccatg
     4141 gccctgcgcc tggaggccct gcccgccggc agcctcggct tcggcgccgc catgcgcgcg
     4201 gccgccgact ccgccctgcg cggcgcccgc ggcaactcgg gcgccatcct cgcccagttc
     4261 ctgcacggcc tggccgagga gctggagggc gaggcgcgga tcggcaccag gcgcttcgcc
     4321 gaggccgcca ggtccgccgc ccggcgggcc cgagccgccc tctccaagcc ccgcgagggc
     4381 accatcgtca ccgtcctcac ggactgggcc gaggcggcgc accgcgtcgc cgcgaagacc
     4441 gacgacttcc tcgtcctcct caaggccgcc ctggaggaag cccgcgcctc cctggcccgc
     4501 acccccgacc tcctccccga gctcaagaag gccggcgtcg tcgacgcggg cgccgagggc
     4561 ttcgtccaca tactggaggg catctgggcc tacgtgagcg gcggccgcct ccgcgaccgc
     4621 gcccgggcag gcaggtccgg cgcggcccgc cacgccgaac gccccgccga cgaggccctc
     4681 cctggcgcgg gatccgagag ggcccatgtc gccgatggcc tggccgagtc cctctccgcc
     4741 gccgaagact ccgtcctccc ggggctctcc gacgcggcgg cggacggcgg ccacgaagag
     4801 agcgccttcc gctactgcac ggagtgcctc gtgacgggcg agggcctgga cctcgaggcc
     4861 ctccgagccg gcctcgttga gctcggcgac tccctcgtcg tcgcaggctc ctccacgctg
     4921 gcccgcgtcc acctccacac cgacaggccc caggccgcct tccgcctcct cgcggaggcg
     4981 ggctccggcg cgagcgccgt cgaaggccag aaggtcgacg acatggagct ccagcgccgc
     5041 ctctccgcgg cgccccggcg cccggccgcc gtcctcacgg acacggcctg cgacctcccc
     5101 gacgccctcg gcctcgagct cctcgccgac cgggccgccg tcctcgtgga gatcggggga
     5161 aaggcctacc tcgaccggga cggcctctcc tccgacgagt tcttcggcat gctccgcgcc
     5221 gaccccgcgg ccccgcttag gagctcccag ccctcacacg cagccttctc ccggaagctc
     5281 gacctcctcc tcgagcgcgc ggacgaggtc gtctatgtcg gcctctccgc ggccctgagc
     5341 ggcaccctgg agggcggccg ccgcgccgcc ggcgagggcc cgcgggccgg ccgggtccgc
     5401 gtcgtcgaca cgaggtccat ctccgtcggc gccgccctcg tcgtccgcg
//
LOCUS       DAOOSJ010000185         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9436_length_5413_cov_6.645017, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000185 DAOOSJ010000000
VERSION     DAOOSJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9436_length_5413_cov_6.645017"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLG14_09285"
     CDS             <1..342
                     /locus_tag="PLG14_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49361.1"
                     /translation="SSLWGDSAGQALRWTHVGLLAAGESLYLANAVTGVGFMEPLEPG
                     LSSRKIHRYAFFGHAFLMAAEATLGFFTTDALRRGDHEAVTKLGVAHAGVGFAIPVLI
                     LGAGAVIELGR"
     gene            578..2200
                     /locus_tag="PLG14_09290"
     CDS             578..2200
                     /locus_tag="PLG14_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX49362.1"
                     /translation="MGKRVLVIGAGIAGLSASSYLQRNGFDTEIFELHDKAGGLCTAW
                     KRGGYTFDGCVHWLMGSGPSSNLHEIWRELGAGDLEYVEWDEYLRVRLSDGDSFVVYT
                     DPARLEAEMLRLGPGDSRVARSVAAKVRLAASLDMPAAMDKLPLGERLALLARLPAAL
                     PLLPWARRSVADLVAPVRSAKLREGFERLFGEAIGEFPAAALFMMLGFMAKKSSGYPI
                     GGSLAFAKAIEAKYISLGGKVRYGARVDEIIVEDGKAVGLRGAWGEERGDYVVSAADG
                     RDTLKRLLRGRYAHPELEASLAEESDGAAEAGAAAAASAGADDGAASSSAPAPRKGAG
                     RAKGKGQARLARYPSLIYVGLGLARDFSGTPHMQSFTLDQSLVLEGGALELKRLGLRL
                     FTFDPTTAPEGKTAAIVMIETRNDAYWSGLRGSDRGAYEREKAETARKVVAALDRFVP
                     GLAESVETVDVATPASFIRYTNNWHGSYEGWLPTGGSLGKRVSKTLPGLRNFHLVGQW
                     TNPGGGLPPCGIGGRRLAQRLCKAEGLRFKAD"
     gene            complement(2202..3461)
                     /locus_tag="PLG14_09295"
     CDS             complement(2202..3461)
                     /locus_tag="PLG14_09295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOX49363.1"
                     /translation="MRTLLVLLSSLNVLMTVFAFATWRLGAGRMKGPGYWFCAFALIA
                     AGTMALSLRGVLPDLVTIVAANSLLFAGISFQSMGVFCFLGRPFPKAAAALLWAAATL
                     FFAYFGLVRPSLAPRLAAFAAYGVVVYAPTALALLRRRGRLKGSALFAGLAFAAFSLI
                     YLYRFATAFAWSGGQDWFANSQSETFYLLAAMLAHCAVAFAELELVNGSLLADLAAAA
                     EEKALLLREMNHRTKNNLALADSLVSLEAGRIEDPAFVSVLEAIRARLRTIGLVHDSL
                     YRADSGSAIRLDEYLGRLLGELGLEALGVKIESRVQAIALSADSALSIGLVVNELVTN
                     CLKYAFPPGGPGTILVSLGSEGGRVRLVVRDDGKGLPESPREGLGGLLVDSLAEQLGG
                     RASRRNEGGAVVELDFPLVDPRRPSSA"
     gene            complement(3590..3757)
                     /locus_tag="PLG14_09300"
     CDS             complement(3590..3757)
                     /locus_tag="PLG14_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49364.1"
                     /translation="MTEEWREVCLPWSSFEKGDFGAFFAFNPARLASVAVAAYKKAFT
                     AALDLRRISFY"
     gene            complement(4005..5279)
                     /gene="serS"
                     /locus_tag="PLG14_09305"
     CDS             complement(4005..5279)
                     /gene="serS"
                     /locus_tag="PLG14_09305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOX49365.1"
                     /translation="MLDYKFIKDNLSAVKKNIADRYMKADADLVVELYDRRNAALRAL
                     EDERKRRNDNAAAMKGKLEPEARQKLIDEGKALKERIAALEAESAEVESRLDEEGRKI
                     PNMAHPAAPVGKEDKDNLEVKRVGEPTKFDFEPKDHVQLGQDLDIIDFDAATRVSGTK
                     FYYLKNEGVILELALVRYALDILIKKGFTLFTTPDVAKTEILEGIGFNPRGAESNVYT
                     VEGEGACLIGTAEITLGGYYSGSVLDRAKLPLKLAGLSHCFRREAGAAGQFSKGLYRV
                     HQFTKVEMFSYCLPEDSNRIHEELRQIEEEIFAGLGVPFRVVDTCSGDLGAPAYRKWD
                     LEAWMPGRNGGEWGEVTSTSNCTDYQARRLNVKYKDEDGKNKYVHMLNGTAIACSRGI
                     IAVLENFQRADGSVAIPPALVPYCGFSEIRRK"
BASE COUNT          788 a         1824 c         1977 g          824 t
ORIGIN      
        1 tcgagcctct ggggcgattc agcgggccag gccctgcgct ggacccacgt cggccttctc
       61 gcggcgggcg aatccctcta cctcgcgaac gcggtcacgg gggtcggctt catggagccc
      121 ctcgagcccg gtctctcgag ccggaagatc caccgctacg ccttcttcgg ccacgccttc
      181 ctcatggccg cggaggcgac gctcggcttc ttcacgaccg acgccctcag gcgcggcgac
      241 cacgaggcgg tcacgaagct cggcgtcgcc cacgcgggcg tcggcttcgc catccccgtc
      301 ctcatccttg gggccggggc ggtgatcgag ctcgggcgat aggcgcgcgc aggactcgtc
      361 ctcggctcgg gcggccccgc gctctcggtc cggacggtct ccggcgacat ctccgtggaa
      421 aggcggcggg gatttgacgc aggcagggga agggcggtat ccttccctca tcccggaagc
      481 gcgccgactt taggcgcgcc cggggccggc gccgcgcatg cgggccggac aggcaaacgc
      541 tcgtggcgcc tccggggccg cgggcgggga gaacgacatg ggaaagcgcg tcctcgtcat
      601 cggcgccggc atcgccggcc tttcggcctc gagctacctc cagcgcaacg gcttcgacac
      661 cgagatcttc gagctccacg acaaggcggg cgggctctgc accgcctgga agcgcggcgg
      721 ctacaccttc gacggctgcg tccactggct catgggctcc ggcccctcca gcaacctcca
      781 cgaaatctgg cgcgagctcg gcgccgggga tctcgagtac gtcgagtggg acgagtacct
      841 gcgcgtccgc ctctccgacg gcgacagctt cgtcgtctac accgatcctg cgaggctcga
      901 ggccgagatg ctgaggctcg gccccgggga ctccagggtc gcgcggtccg tcgcggccaa
      961 ggtcaggctg gccgccagcc tggacatgcc ggcggcgatg gacaagctgc cgctcggaga
     1021 gagactcgcc ctcctggcca ggctgcccgc ggccctgccc ctccttccct gggccaggcg
     1081 ctccgtcgcc gacctcgtcg cgccggtcag gagcgcgaag ctccgcgagg gcttcgagcg
     1141 cctcttcggc gaggccatcg gcgaattccc cgccgccgcc ctcttcatga tgctcggctt
     1201 catggccaag aagagctcgg gctatccgat aggaggctcc ctagccttcg cgaaggcgat
     1261 cgaggccaag tacatctcgc tcggcgggaa ggtccgctac ggcgcgcgcg tcgacgagat
     1321 catcgtcgag gatggcaagg ccgtcggcct ccgcggcgcc tggggcgagg agaggggcga
     1381 ctacgtggtc tccgccgccg acggccgcga cacgctgaag cggctccttc gcggccgcta
     1441 cgcgcatccc gaactcgagg cctccctcgc cgaggagtcg gacggcgcgg cggaggccgg
     1501 cgctgcggcc gcggcctcgg ccggcgctga cgacggcgcg gcctcgtcgt cggccccggc
     1561 tccgcggaag ggtgcgggcc gtgcaaaggg caagggccag gcgcggctcg cccgctatcc
     1621 ctcgctcatc tacgtgggac tcggcctcgc gcgggacttc tccgggacgc cccacatgca
     1681 gtccttcacg ctggaccaga gcctcgtcct ggagggcggc gccctcgagc tcaagcgcct
     1741 cggcctccgg ctcttcacct tcgacccgac cacggcgccg gaggggaaga cggcggccat
     1801 cgtgatgatc gagacccgca acgacgccta ttggtccggc ctgcgcggga gcgaccgcgg
     1861 cgcctacgag agggagaagg cggagaccgc gcggaaggtc gtcgccgccc tggaccgctt
     1921 cgtgcccggc ctcgccgagt cggtggagac cgtcgacgtc gcgacgcccg cgagcttcat
     1981 ccgctacacg aacaactggc acgggagcta cgagggctgg ctgcctacgg gcggctccct
     2041 cgggaagcgg gtctccaaga ccctccccgg cctccgcaac ttccatctcg tcgggcagtg
     2101 gacgaatccg ggcggcggcc ttcctccctg cggcatcggc ggccgcaggc tcgcccagcg
     2161 gctctgcaag gcggagggcc tgcgcttcaa ggccgactag gctaggccga cgaggggcgg
     2221 cgcgggtcga cgagggggaa gtcgagctcg acgacggcgc cgccctcgtt cctccgcgag
     2281 gccctgccgc cgagctgttc ggcgagggag tcgacgagga gtccgcctag cccctcgcgc
     2341 ggggattctg gcaggccctt cccgtcgtcc cgtacgacga ggcggacccg gccgccttcc
     2401 gagccgaggc tcacgaggat ggttcccggc ccccccgggg ggaaggcgta cttgaggcaa
     2461 ttggtgacaa gctcgttcac gacgaggccg atcgagaggg cggagtcggc ggacagcgcg
     2521 atggcctgga cccgggactc gatcttgacg cccagggcct ccagcccgag ctcgccgagt
     2581 aggcggccca ggtactcgtc gagccgtatg gccgagcccg aatcggcgcg gtagaggctg
     2641 tcgtggacga ggcctatcgt ccggagccgg gcgcggatcg cctccaggac ggagacgaag
     2701 gcgggatcct cgatcctgcc cgcctccagg gagacgaggg agtcggcgag ggcgaggttg
     2761 ttcttggtcc ggtggttcat ctcgcggagg aggagggcct tctcctccgc cgccgccgcg
     2821 aggtcggcga ggaggctgcc gttcacgagc tccagctcgg cgaaggcgac ggcgcagtgg
     2881 gcgagcatgg cagccaagag gtagaaggtc tcgctctgcg agttggcgaa ccagtcctgg
     2941 ccgccggacc aggcgaaggc ggtggcgaag cggtagagat agatgaggga gaaggccgcg
     3001 aaggccaggc cggcgaatag ggcgctgccc ttgagccgcc cgcgcctcct gaggagggcc
     3061 agggcggtcg gcgcgtagac gacgacgccg taggccgcga aggcggcgag gcgcggcgcg
     3121 aggctgggcc ggacgaggcc gaagtaggcg aagaagagcg tcgcggcggc ccagagcagg
     3181 gcggcggctg ccttcgggaa gggcctgccc aggaagcaaa agacgcccat ggactggaag
     3241 ctgatgccgg cgaagagcag ggagttggcc gcgacgatgg tgacgaggtc gggcaggacg
     3301 ccccgcaggg agagcgccat cgtccccgcc gcgatcaggg cgaaggcgca gaaccagtat
     3361 cccgggccct tcatccggcc cgccccgagg cgccaggtcg cgaaggcgaa gacggtcatg
     3421 aggacgttga gggaactgag gaggacgagc agcgtcctca tgtccaggag cggcaaccgc
     3481 gcgacctcct tgtcatagcc tccgcgtctc tcgccctgaa agagtagggg aggcgcggga
     3541 ggctgtcaag gcgagttttc cggcgggctt ctcttcggcc ttggggccgt caatagaacg
     3601 agatgcgccg caggtccagg gccgcggtga aggctttctt gtaggccgcc acggcgacgc
     3661 tcgcgagcct ggccgggttg aaggcgaaga aggctccgaa atcgcccttc tcgaagctgc
     3721 tccaggggag gcatacctcc cgccattcct ccgtcacggg aatgcgggcc aggaagaagg
     3781 accagggcag gacgttggct gccgcccatg gcgaggtggg ggaggccgcc ctcgctccgg
     3841 atgccgaccg tcatgtccga ctcgccgccc atgacccggt ccgtgaatcc ctcccagcgc
     3901 gcgccaagag gcgattcggg cccggagaaa tcgtcgatca acagctcggg catgggcacc
     3961 tccgctcgga gcgcttcgtc ggtccgcctc ggcttccctc ccgcctactt ccgcctgatc
     4021 tcgctgaagc cgcagtaggg gaccagggcc ggagggatgg cgaccgagcc gtccgcgcgc
     4081 tggaagttct cgaggacggc gatgatgccg cggctgcagg cgatcgccgt gccgttgagc
     4141 atgtggacgt acttgttctt gccgtcctcg tccttgtact tcacgttgag gcgccgggcc
     4201 tggtagtcgg tgcagttcga ggtcgaggtg acctcgcccc actcgccgcc gttgcggccg
     4261 ggcatccagg cctccaggtc ccacttgcgg taggcggggg cgcccaggtc gcccgagcag
     4321 gtgtcgacca cgcggaaggg gacgccgagg ccggcgaaga tctcctcctc gatctggcgg
     4381 agctcctcgt ggatccggtt ggagtcctcg ggcaggcagt aggagaacat ctccaccttc
     4441 gtgaactggt ggacgcggta gaggcccttg gagaactggc ccgcggcgcc cgcctcgcgg
     4501 cggaagcagt gggagaggcc ggcgagcttc aggggcagct tcgccctgtc caggacgctt
     4561 cccgagtagt agccgcccag ggtgatctcg gcggtgccga tgaggcaggc gccctcgccc
     4621 tccacggtgt agacgttgga ctcggcgccg cgggggttga agccgatgcc ctcgaggatc
     4681 tcggtcttcg cgacgtcggg ggtcgtgaag agggtgaagc ccttctttat gaggatgtcc
     4741 agggcgtagc gcacgagggc gagctcgagg atgacgccct cgttcttgag gtagtagaac
     4801 ttggtgccgg agacgcgggt cgcggcgtcg aagtcgatga tgtccaggtc ctgtccgagc
     4861 tggacgtggt ccttgggctc gaagtcgaac ttcgtgggct cgccgacgcg cttgacctcg
     4921 aggttgtcct tgtcttcctt gccgacgggg gccgcggggt gggccatgtt cgggatcttg
     4981 cggccctcct cgtccaggcg ggactccacc tcggcggact cggcctccag ggcggcgatg
     5041 cgctccttca gggccttgcc ctcgtcgatg agcttctggc gggcctcggg ctccagcttg
     5101 cccttcatcg cggcggcgtt gtcgttccgc ctcttccgct cgtcctcgag ggcgcgcagg
     5161 gcggcgttcc ggcggtcgta gagctcgacg acgaggtcgg cgtcggcctt catgtagcgg
     5221 tcggcgatgt tcttcttgac cgcgctcagg ttgtccttga tgaacttgta gtcgagcatg
     5281 atgggccata ctagcgcggc gggcccgggg aggcaagcgg gccgggcccg cccctgtcgc
     5341 ggtcagcggg tccgcccctg ccgggctcgc ccttgccggg cggccgcctt ttgcggtagc
     5401 attcccgcat gag
//
LOCUS       DAOOSJ010000186         5381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9511_length_5381_cov_5.901427, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000186 DAOOSJ010000000
VERSION     DAOOSJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9511_length_5381_cov_5.901427"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG14_09310"
     CDS             <1..237
                     /locus_tag="PLG14_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HflK protein"
                     /protein_id="HOX49366.1"
                     /translation="VEVAQGYATERVNRAKGDVARFNSVYEEYRKAPEVTRQRLYYEM
                     MEEVFKGASGVELIDKRFTNFLPLKNLDQKGAVK"
     gene            234..1214
                     /gene="hflC"
                     /locus_tag="PLG14_09315"
     CDS             234..1214
                     /gene="hflC"
                     /locus_tag="PLG14_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOX49367.1"
                     /translation="MKRLAVILAVAVVALVVFVALGPFYVVREGEQALVVRLGKIVSV
                     QTEAGLKIRSPFVDNVVKYPKKILSWDGMPERIQTKENQYIWVDTTARWKISDPVKFY
                     ESVGTIEGGYGRLDDVIDSSVRTVISSNFLREAVRNSNRLIGTETKESFQTGDSEGSA
                     ALDQLTQSDISYEGIEKGRDKLSRDMLTAIQGIVPSYGIEVLDIVPRQIKYSDELTES
                     VYQRMIKERNQIAQAFRSYGEGKKAEWFGKLENEKRSILSGAYQKAETAKGKADAEAS
                     RIYADAYGRDPAFFDFWRAVESYRQTLPGFNKTISTDMDYFRYLYGTKGR"
     gene            1348..3021
                     /locus_tag="PLG14_09320"
     CDS             1348..3021
                     /locus_tag="PLG14_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOX49368.1"
                     /translation="MSIRLKIIFVVLPLIIVAVVLAGMSSYFVAASSVTRIATEFLSF
                     KASELEKYAESQWNLLAENGYVGRPDMEAAARAAVESFARSILRSPTEAIVAVDAEGT
                     VAMRAGGSGGSALEPLPEEKAALAAWAAEGKRGFVQARLAGKDRVASAFAFKPFGWEI
                     LVTEERASFYGEVEAIARTSLEILAAAAAAAIVLLLLFARVLTKPIVEVVAAMRRIIA
                     SNDLSERVPVEYKDEIGQLSHTFNIMLGELGKAYDQIKRFAFDAVVAQKREMKVRNIF
                     QLYVPKDVIDQVFMNPEGMLVGDNRVVSILFSDIRSFTSISEKMAPDDLVTALNRYFS
                     GMVDVIMARGGVVDKYIGDAIMAFFGAPVRHEDDALQSVLAGLEMAEALDAFNAGQRA
                     SGGPEFHIGVGINYGVVTVGNIGCEKKMNYTVIGDMVNLASRLEGLTKMYHQPLLFSE
                     SVYLKVKDALPCRQVDTVAVKGKTKGVRIYTARRRVEGPEAEAWALHSSALERYYDRD
                     FRGAIAGLEKVQGLLKDDYASEIFLERARRYDKAPPSADWVGVEVMTEK"
     gene            3018..4262
                     /locus_tag="PLG14_09325"
     CDS             3018..4262
                     /locus_tag="PLG14_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49369.1"
                     /translation="MMKAPRPSPRRAASLAFVLCLAAASLPAEETAAPQAPLDPGRGV
                     VVLAVGSGGEEARRFAEALAVSLAREAEATGLSASRGAPIDAAMPEPQAAKGAAAAAG
                     ARWVFVARASVESRRLLWRAAVYDGSDGALYGADAYSAYAGLSALPLLEASAKGAAVA
                     AKTARDAPERPSPIARRLSFSSPDEGAEVFFGLPGKDAASAGFVAEGLLEAPYFPFMP
                     GERLEIGVAKEGYWPRGLSLKVKAEPEPFRIPPLFKETSLAWGATYGVGRLLGAAASL
                     RWYPLPDRVYLRAENALWAAYDFRPGSKPVLHDELRLGAAAYLFFDPEARFRSSLGLG
                     LSGIATLLTSPGLADPFAFDLCLEPVFLTLEWHLADWALVLEQRFPYSLGLDSGLLPR
                     QWLELGGSGPPLFVSLGAIFKL"
     gene            4397..5260
                     /locus_tag="PLG14_09330"
     CDS             4397..5260
                     /locus_tag="PLG14_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49370.1"
                     /translation="MNVEASLDIAPSLAASGLSGPYTVERSERLTATGGESALFVFAH
                     GSNGSRLLILDPESLEVRAVRTSTDFSRFLGADDAGNFICGASRVAPGTFVVTSAIYT
                     FIPTQAADFPRSVAFIMTEGGGTYTSLALRNKNDSSQIGLSTPIAPATYFELLDAETW
                     GGHTFLLARRNDPSGGLIAVDLPGDTPAELALLLDTAFLSIKPSVAITTVAAADVDRA
                     WLTEGGIVAFSRDRDSRVVRYAYGSGEELDHKSVRSDWKEGASFDPSGDSWYYYDRYD
                     SRFYRLRSWWK"
BASE COUNT          847 a         2019 c         1634 g          881 t
ORIGIN      
        1 gtcgaggtgg cccagggcta cgcgaccgag cgcgtcaacc gcgccaaggg cgacgtggcc
       61 cgcttcaact ccgtctacga ggagtaccgc aaggcgccgg aagtcacgcg ccagcgcctc
      121 tactacgaga tgatggagga ggtcttcaag ggcgcctccg gcgtcgagct catcgacaag
      181 cgcttcacca acttcctccc gctcaagaac ctggaccaga agggggccgt gaaatgaagc
      241 gactcgccgt catcctcgcc gtcgccgtcg tcgccctcgt cgtcttcgtg gccctcgggc
      301 ccttctacgt cgtcagggag ggcgagcagg ccctcgtcgt gcgcctcgga aagatcgtct
      361 ccgtccagac ggaggccggc ctcaagatca ggtcgccctt cgtcgacaac gtcgtgaagt
      421 acccgaagaa gatcctctcc tgggacggca tgcccgagcg catccagacc aaggagaacc
      481 agtacatctg ggtggacacc accgcccgct ggaagatctc cgaccccgtg aaattctacg
      541 agtccgtcgg caccatcgaa ggcggctacg ggcgcctgga cgacgtcatc gactcctcgg
      601 tgcgcaccgt catctcctcc aacttcctcc gcgaggccgt gcgcaactcg aaccgcctca
      661 tcggcacgga gaccaaggag agcttccaga ccggcgactc cgagggctcc gccgccctcg
      721 accagctcac ccagtcggac atctcctacg agggcatcga gaagggccgc gacaagctct
      781 cccgcgacat gctgaccgcc atccagggca tcgtcccctc ctacggcatc gaggtcctgg
      841 acatcgtgcc ccgccagatc aagtactccg acgagctcac ggagagcgtc taccagcgca
      901 tgatcaagga gcggaaccag atcgcccagg ccttccggag ctacggcgag ggcaagaagg
      961 ccgagtggtt cggcaagctg gagaacgaga agcgctccat cctctccggg gcctaccaga
     1021 aggccgagac cgcgaagggc aaggccgacg ccgaggcctc caggatctac gccgacgcct
     1081 acggccgcga cccggccttc ttcgacttct ggcgcgccgt cgagtcctac aggcagaccc
     1141 tgcccggctt caacaagacc atctccacgg acatggacta cttccgctac ctctacggga
     1201 ccaaggggcg ctaatgcccc cgcccggccg gccctcgagg ccggccgccg gccccagggc
     1261 cttcccggcc gatcctttcc aaggatctcg cccccgggaa ggcctttttt gttgcgcgag
     1321 gcccgcggag cggagtatga tggatgcatg agcatacggc tcaagatcat cttcgtcgtc
     1381 ctgcccctca tcatcgtcgc cgtcgtgctc gccggcatgt cgtcgtattt cgtcgccgcc
     1441 tcctccgtga cgcggatcgc gacggagttc ctctccttca aggcctccga gctcgagaag
     1501 tacgcggaga gccagtggaa cctcctcgcg gagaacggct acgtgggcag gcccgacatg
     1561 gaggccgcgg cgcgggccgc ggtcgagtcc ttcgcgcggt ccatcctccg cagccccacg
     1621 gaggccatcg tcgccgtgga cgcggaaggg accgtcgcga tgcgcgccgg cggctcgggc
     1681 ggctcggccc tggagcccct tcccgaggag aaggccgccc tcgccgcctg ggccgcggag
     1741 ggcaagcgcg gcttcgtcca ggccaggctc gccggcaagg accgcgtcgc ctcggccttc
     1801 gccttcaagc ccttcggctg ggagatcctg gtcaccgagg agcgggcctc cttctacggc
     1861 gaggtcgagg cgatagcccg cacctccctg gagatcctcg cggccgccgc ggccgcggcg
     1921 atagtcctcc tcctcctctt cgcccgcgtg ctcacgaagc ccatcgtgga ggtcgtcgcc
     1981 gcgatgcggc gcatcatcgc ctccaacgac ctctccgagc gggtccccgt cgagtacaag
     2041 gacgagatcg gccagctctc ccacaccttc aacatcatgc tcggcgagct cggcaaggcc
     2101 tacgaccaga tcaagcgctt cgccttcgac gccgtcgtgg cccagaagcg cgagatgaag
     2161 gtcaggaaca tcttccagct ctacgtgccc aaggacgtca tcgaccaggt cttcatgaac
     2221 cccgagggca tgctcgtcgg cgacaaccgc gtcgtgtcca tcctcttctc cgacatcagg
     2281 agcttcacct ccatctccga gaagatggcg cccgacgacc tggtcaccgc cctcaaccgc
     2341 tacttctccg ggatggtcga cgtgatcatg gcaaggggcg gcgtcgtcga caagtacatc
     2401 ggcgacgcga tcatggcctt cttcggcgct ccggtccgcc acgaggacga cgccctccag
     2461 tccgtcctgg ccggcctcga gatggcggag gccctggacg ccttcaacgc cggccagcgc
     2521 gcctccggcg gcccggagtt ccacatcggc gtgggcatca actacggcgt cgtcaccgtc
     2581 ggcaacatag gctgcgagaa gaagatgaac tacacggtca tcggcgacat ggtgaacctg
     2641 gcctcgcgcc tggagggcct gaccaagatg taccaccagc ccctcctctt ctccgagagc
     2701 gtctacctca aggtgaagga cgcccttccc tgccgccagg tcgacacggt cgccgtcaag
     2761 ggcaagacca agggcgtccg catctacacc gcccgccgca gggtggaggg acccgaggcg
     2821 gaggcctggg ccctccactc ctcggccctg gagcgctact acgaccgcga cttccgcggc
     2881 gccatcgccg gcctggagaa ggtccagggc ctcctcaagg acgactacgc ctcggagatc
     2941 ttcctcgagc gcgcgcgccg ctacgacaag gccccgccct ccgcggactg ggtcggcgtc
     3001 gaggtcatga cggagaaatg atgaaggccc caaggccctc gccccggcgc gcggcgtcgc
     3061 tcgccttcgt cctctgcctg gcggcggcgt cgctgcctgc cgaggagacg gccgcccccc
     3121 aagcccccct ggatccgggg cggggcgtcg tcgtcctcgc cgtgggctct ggcggcgagg
     3181 aggcgcgccg cttcgccgag gccctcgccg tctccctggc ccgcgaggcc gaggctacgg
     3241 gcctttccgc ctcgcgcggc gcgccgatcg acgcggcgat gcccgagccc caggccgcca
     3301 agggcgcggc cgccgccgcc ggcgcgcgat gggtcttcgt ggcccgcgcc tccgtcgagt
     3361 cgcggcgcct cctctggcgg gccgccgtct acgacggctc ggacggagcc ctctacggcg
     3421 ccgacgccta ttccgcctac gcgggccttt cggccctgcc cctcctcgaa gcctcggcca
     3481 agggagcggc tgtcgcggcc aagaccgcca gggacgcgcc ggagcggccc tctcccatag
     3541 cccgccgcct gtccttctcc tcccccgacg agggcgccga ggtcttcttc ggcctgccgg
     3601 ggaaggacgc tgcctcggca ggcttcgtcg ccgagggcct cctggaggcg ccctacttcc
     3661 ccttcatgcc cggcgagcgc ctggagatcg gcgtcgccaa ggaaggctac tggccgcgcg
     3721 ggctctccct caaggtgaag gccgagcccg agccattccg cattcccccc ctcttcaagg
     3781 agaccagctt ggcctggggc gcgacctacg gcgtgggccg cctcctcgga gcggccgcct
     3841 ccctgcgctg gtatcccctc ccggaccgcg tctacctccg cgcggagaac gccctctggg
     3901 cggcctacga cttccgcccg ggctccaagc ccgtcctcca cgacgaactc aggctcggcg
     3961 ccgccgccta cctcttcttc gaccccgagg cccgcttcag gtccagcctg ggcctgggcc
     4021 tttccgggat agccaccctc ctcacgagcc caggcctggc cgatcccttc gccttcgacc
     4081 tctgcctcga gcccgtcttc ctcaccctgg agtggcacct cgctgactgg gccctcgtcc
     4141 tcgagcagcg cttcccctac tccctcggcc tggactcagg cctcctcccc cgccaatggc
     4201 tggagctcgg cggctcgggt ccgccgcttt ttgtctccct gggagctata ttcaagctat
     4261 gagtagcctc atgcgccgag cagccagccg tcgccccgcc gcgcggggag ccgcttacgc
     4321 ctgcggcgcc cttgcgctcg cccttctcct ctccgcatgc gacttcatcg gcctggacat
     4381 gtttccgccg gagctcatga atgtcgaggc gagcctggac atcgccccga gcctggccgc
     4441 ctcgggactc tccggcccct acaccgtgga gcgttccgag aggctcaccg cgacgggagg
     4501 cgagtccgcc ctcttcgtct tcgcgcacgg ctccaacggc tcccgcctcc tcatcctgga
     4561 tcccgagagc ctcgaggtac gcgccgtacg cacaagcacg gatttctccc gtttccttgg
     4621 cgccgacgat gcgggcaatt tcatctgtgg cgcttcccgc gtcgcgccag gcactttcgt
     4681 cgtcacctcg gcaatctata ccttcatacc cacacaggcc gccgattttc cccgctcggt
     4741 cgcgtttatc atgaccgaag ggggcggcac ctacaccagc ctcgcccttc ggaacaaaaa
     4801 tgacagcagt caaatagggt tgtcgacccc catcgccccg gccacctatt ttgagctcct
     4861 cgacgccgag acctggggcg gacatacctt cctccttgcc aggcgaaacg atccttcggg
     4921 cggtctcatc gcagtcgatc tgccgggcga cacccccgcg gaattagcac tcctattgga
     4981 taccgcattt ctctccatta agccgtccgt cgcgatcacg acggtcgccg ccgccgatgt
     5041 cgatcgagca tggctcaccg aaggcgggat cgtggccttc tcccgggaca gggactcgcg
     5101 cgtcgtccgc tacgcctatg gaagcggaga agagctggac cacaagagcg tccgcagcga
     5161 ctggaaggaa ggagcctcct ttgatccctc gggcgacagc tggtactact acgaccgcta
     5221 cgacagccgt ttttaccggc tgaggagctg gtggaaatga ggcgtttcgc ggccatgctc
     5281 cttctcgtcc tcgcgtcggc cgccgccggc gcggaggatc ggcgaagaat actcgtcgcc
     5341 ttctccgcgc ctggctatga agcggccaag gtcgagtcct t
//
LOCUS       DAOOSJ010000187         5377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9520_length_5377_cov_7.040962, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000187 DAOOSJ010000000
VERSION     DAOOSJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_5377_cov_7.040962"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLG14_09335"
     CDS             <1..363
                     /locus_tag="PLG14_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HOX49371.1"
                     /translation="KYYELGEDDVVFTMFTDSMEMYGSRLLELTAERGKYDQRQADRD
                     YDRLMGVSVDHVLELSQVDKRRVHQLKYFTWIEQLGKDLGELRAQWDDYRAYWGGLRS
                     EVGALDAMIEDFNKEVLR"
     gene            360..1721
                     /locus_tag="PLG14_09340"
     CDS             360..1721
                     /locus_tag="PLG14_09340"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOX49372.1"
                     /translation="MILLKDCLRVVRPHGADLAGVDVLVDGNRITRIAKAIPAPAGAE
                     VVDASGLVCAPGLVNTHHHFYQTLTRNVPAVQDAKLFDWLVYLYEVWKGVDEEAVYWS
                     SMLAMAELAKTGCTLTTDHHYLYPRGFKDDIPRLQFEAAARIGLRFAPTRGSMSRSKK
                     DGGLPPDSVVQDEDEILAHCEATIRKYHDPAEDAMRKVALAPCSPFSVSEKSMRDAAD
                     LARRHKVRLHTHLAETRDEDDFCVATLGRRPLKVMEDCGFVGPDVWYAHGIFFNDEEL
                     DLLARTRTGVAHCPSSNMRLGSGICRVREMLDRGIPVGLAVDGSASNDSSDMLGEARQ
                     ALLLQRVRYGSAGITAKEVWGVATEGGARVLGYEKAGRIEEGWLADIALFDVMKMEYA
                     GALSDPSAALLFSGYDHGAEHVIANGRFVVKDRRLVGADEEEIRRNADLASRRLLEKA
                     GVA"
     gene            1723..2538
                     /locus_tag="PLG14_09345"
     CDS             1723..2538
                     /locus_tag="PLG14_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOX49373.1"
                     /translation="MRADTYLRPASLEEALAAKASKPEAVFLAGGTYLLSSDRGAACG
                     RDYVDVSRLLPRALERRGAELVVGAGATFQDLLDSAAAPASLKAAAASMAARNVRNRA
                     TVGGNLGAGKSCASLVPILLVLDARVEAAQGPGAGRVSLPLAAWLERPQGLLLDVILP
                     LEAGERRSFQRWGRSACDVSVLTAAASYRLEGDGTARGLAVAAGGLGPRARRFPEIEQ
                     LFEGKRLPSAPEMEEAIRPLLSPIDDLRGSAAFKRLRGAALIADAIAKAEAEA"
     gene            2535..>5377
                     /locus_tag="PLG14_09350"
     CDS             2535..>5377
                     /locus_tag="PLG14_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HOX49374.1"
                     /translation="MTVKFTLNGRPVALEAGAGESLKALLKRRGVISVRDGCDGEGSC
                     GLCAVLLDGKLVNSCLVLAAQAEGSQVLTLEHFSKDRKLGIIQRALVDASCVQCGYCT
                     PAVVLAVHELLERTKSPTKAEVKDALSGTMCRCTGYEQFHAAVRIASKRLSDPVYTEA
                     LAPEFKPELRHVGKDREKIDAAALALGEKAFVEDRVEPGACHLRMLGSPLAHAWIKSI
                     DTGAALAMPGVVAVLTHENCPDKVYTTAGQGFPEPSPYDQRMFSRKVRHVGDRVAAVL
                     AETPEEAEAALAAIKVEYEPLEPVLSIDAAKAPGAPVVHSGEVRYVVGEPPKGSSTKG
                     DGRDDPITYQFPLHADPHRNLAASAADGIGDMDKGFAEADVVIERKYRGNRVQSTPLE
                     PHVVYAKVDGGRLVLHASTQVPWHVRRIVAGVVGIPENKVRVVKERVGGGFGAKQDIV
                     VEDVAGFLAWTTGRPVYFRYNREQEFVASRTRHAMEVTVKLGAKRDGKLTAILMRLDA
                     DTGPYGQHCLTVPMNAISKSLPLLLCENARFEVKSYYTNLSPAGAYQGYGAPKGSFAL
                     QTALAELAAELGIDQLEMIEKNRVRSGARIEILRSLGEGREGQAVTLGECGLGEMIER
                     GKKAFRWGEPKPASGDPDWKIGRGAVVIQQGSGLPGLDAANAQVRLLADGTVILLSGG
                     ADLGTGLDTVTAKAVAEILGLEMADVAVVSGDTDATPFDKGAYASSGTYFSGNAALLA
                     AKDLRDKALAVAAGILGEPVADLRLAHRGMVKGKSGEISLAKLAHMATQGEGHGELCG
                     SACFKTDHAAFPYGAHFVEAAVNARSGEVRVRRYHAYQDCGTPINPALAKGQIFGGCL
                     KAIGHSLYEEMVYDEKGRCLNPRFLDYKIPSIYEVPEDYVVELVPVEDEVGPFGGKSV
                     SEIALNGAAPAIAIAIHDAVGAWCREWPFTPERV"
BASE COUNT          830 a         2035 c         1790 g          722 t
ORIGIN      
        1 aagtactacg agctcggcga ggacgacgtc gtcttcacga tgttcaccga ctccatggag
       61 atgtacggct cccgcctcct cgagctcacc gccgagcgcg gcaagtacga ccagcgccag
      121 gccgaccgcg actacgaccg cctcatgggc gtctccgtcg accacgtcct ggagctctcc
      181 caggtggaca agcgccgcgt gcaccagctc aagtacttca cctggatcga gcagctcggg
      241 aaggacctcg gggagctccg cgcccagtgg gacgactacc gcgcctactg gggcggcctc
      301 cgctccgagg tcggcgccct cgacgcgatg atcgaggact tcaacaagga ggtgcttagg
      361 tgatcctcct caaggattgc ctccgcgtgg tcaggcccca cggagccgac ctcgcgggcg
      421 tggacgtcct cgtcgacggc aaccgcataa cgcggatcgc caaggcgatc ccggcgcccg
      481 cgggcgccga ggtcgtcgac gcctcgggac tcgtctgcgc gccgggcctc gtcaacaccc
      541 accaccactt ctaccagacc ctcacgcgga acgtcccggc cgtgcaggac gccaagctct
      601 tcgactggct cgtctacctc tacgaggtct ggaagggcgt cgacgaggag gcggtctact
      661 ggtcgagcat gctcgcgatg gccgagctcg cgaagacggg ctgcaccctc acgaccgacc
      721 accactacct ctacccccgc ggcttcaagg acgacatccc gaggctccag ttcgaggcgg
      781 cggccaggat cggcctccgc ttcgcgccga cccgcggctc catgtcccgc tccaagaagg
      841 acggcggcct gcctcccgac tccgtcgtcc aggacgagga cgagatcctc gcgcactgcg
      901 aggcgacgat ccggaagtac cacgacccgg cggaggacgc gatgcgcaag gtcgccctcg
      961 cgccctgctc gcccttctcc gtctccgaga agtcgatgcg ggacgcagcc gacctcgcgc
     1021 gccgccacaa ggtcaggctc cacacccacc tggcggagac gagggacgag gacgacttct
     1081 gcgtcgcgac cctgggccgc cgccccctca aggtgatgga ggactgcggc ttcgtcggac
     1141 ccgacgtctg gtacgcccac ggcatcttct tcaacgacga ggagctcgac ctcctcgccc
     1201 gcaccaggac cggcgtcgcc cactgcccct cctccaacat gcgcctgggc tccggcatct
     1261 gccgcgtgcg ggagatgctc gaccgcggca tacccgtcgg cctcgcggtc gacggctccg
     1321 catcgaacga cagctccgac atgctcggcg aggcccgcca ggccctcctc ctccagcgcg
     1381 tgcgctacgg ctccgccggc atcaccgcga aggaggtctg gggcgtggcc accgagggcg
     1441 gcgcgcgcgt cctcggctac gagaaggccg gccggatcga ggaaggctgg ctcgccgaca
     1501 tcgccctctt cgacgtgatg aagatggagt acgcgggggc cctctcggac ccctccgcgg
     1561 ccctcctctt ctcgggttac gaccacggcg ccgagcacgt catcgcgaac ggccgcttcg
     1621 tggtcaagga ccggcgcctc gtcggcgccg acgaggagga gatcaggcgg aacgccgacc
     1681 tcgcgtcccg ccgcctcctc gagaaagcgg gagtcgcctg acatgagagc cgacacctat
     1741 ctgcggccgg cctccctgga ggaggccctg gccgccaagg cctcgaagcc cgaggccgtg
     1801 ttcctcgcgg gcgggaccta cctcctctcg agcgaccgcg gcgcggcctg cgggagggac
     1861 tacgtcgacg tgtcccgcct ccttccgcgc gccctggagc gccggggagc ggagctcgtc
     1921 gtcggggcgg gggcgacctt ccaggacctc ctcgactccg ccgccgcccc ggcctccctc
     1981 aaggccgcgg ctgcctccat ggcggcgcgc aacgtcagga accgtgccac cgtgggcggc
     2041 aacctgggcg cgggcaagtc ctgcgcgagc ctcgtcccga tcctcctcgt cctggacgcc
     2101 cgcgtggagg ccgcgcaggg gcccggagcc gggcgcgtct cccttcccct cgcggcctgg
     2161 ctcgagcgtc cccagggcct cctcctcgac gtcatcctgc ccctggaggc gggagagcgc
     2221 aggtccttcc agcgctgggg caggagcgcc tgcgacgtct ccgtcctgac cgccgcggcc
     2281 tcctacaggc tcgagggcga cgggacggcc aggggcctcg cggtcgccgc cggaggcctg
     2341 ggcccccggg cccgccgctt cccggagatc gagcaactct tcgaggggaa gaggctcccc
     2401 tccgctcccg agatggagga ggcgatccgc cccctcctct ccccgatcga cgacctcagg
     2461 gggagcgccg ccttcaagcg cctccgcggg gccgcgctga tcgccgacgc gatcgccaag
     2521 gcggaggccg aagcatgaca gtcaagttca ccctgaacgg ccggcccgtc gccctcgagg
     2581 ccggggccgg cgagagcctc aaggccctcc tcaagcgccg cggcgtcatc tcggtccgcg
     2641 acggctgcga cggcgagggc tcctgcggcc tctgcgccgt cctcctggac ggcaagctcg
     2701 tcaactcctg cctggtcctc gccgcccagg ccgagggctc gcaggtcctc accctggagc
     2761 acttctccaa ggacaggaag ctcgggatca tccagcgggc cctggtggac gcctcctgcg
     2821 tccagtgcgg ctactgcacc ccggccgtcg tcctcgccgt ccacgagctc ctggagcgga
     2881 cgaagagccc taccaaggcc gaggtcaagg acgccctctc cggcacgatg tgccgctgca
     2941 cgggctacga gcagttccac gccgccgtgc gcatcgcctc caagcgcctg tcagatcccg
     3001 tgtacacgga ggccctggcc cccgagttca agcccgagct ccgccacgtc ggcaaggacc
     3061 gcgagaagat cgacgcggcg gccctggccc tgggcgaaaa ggccttcgtc gaggacaggg
     3121 tcgagcccgg cgcctgccac ctcaggatgc tcggcagccc cctcgcgcac gcctggatca
     3181 agtcgatcga cacgggcgcc gccctcgcga tgcccggcgt cgtcgccgtc ctcacccacg
     3241 agaactgccc cgacaaggtc tacacgacgg cgggccaggg cttccccgag ccctcgccct
     3301 acgaccagcg catgttctcc cggaaggtcc gccacgtcgg cgaccgcgtc gccgcggtgc
     3361 tcgcggagac gcccgaggag gcggaggcgg ccctggccgc catcaaggtc gagtacgagc
     3421 ccctcgagcc cgtcctctcc atcgacgccg ccaaggcgcc cggcgcgccg gtcgtccact
     3481 cgggcgaggt gcgctacgtc gtcggcgagc cgcccaaggg cagctcgacg aagggcgacg
     3541 gcagggacga tcccataacg taccagttcc ccctccacgc cgacccgcac cgcaacctcg
     3601 cggcgagcgc tgccgacggg attggcgaca tggacaaggg cttcgccgag gccgacgtcg
     3661 tgatcgagcg gaagtaccgc ggcaaccgcg tccagtcgac gcccctcgag ccccacgtgg
     3721 tctacgcgaa ggtcgacgga gggcgcctgg tcctccacgc ctccacccag gtgccctggc
     3781 acgtcaggcg catcgtcgcg ggcgtcgtcg gcattccgga gaacaaggtg cgcgtcgtga
     3841 aggagcgcgt gggcggaggc ttcggcgcca agcaggacat cgtcgtcgag gacgtcgcgg
     3901 gcttcctggc ctggaccacc ggaaggccgg tctacttccg ctacaacagg gagcaggagt
     3961 tcgtcgcctc gcgcactcgg cacgcgatgg aggtgacggt gaagctcggc gcgaagcgcg
     4021 acgggaagct caccgccatc ctcatgcgcc tggacgcgga cacgggcccc tacggccagc
     4081 actgcctcac cgtgcccatg aacgccatct ccaagagcct tcccctcctc ctctgcgaga
     4141 acgcccgctt cgaggtgaag tcctactaca cgaacctctc ccccgccggc gcctaccagg
     4201 gctacggcgc gcccaagggc tccttcgccc tccagaccgc cctggcggag ctggcggccg
     4261 agctcggcat cgaccagctg gagatgatcg agaagaaccg cgtccgctcg ggcgcgcgca
     4321 tcgagatcct gcggagcctg ggcgagggcc gcgaaggcca ggcggtgacg ctcggcgagt
     4381 gcggcctggg cgagatgata gagcgcggca agaaggcctt ccgctggggc gagcccaagc
     4441 ccgcctccgg ggaccccgac tggaagatcg gccgcggcgc ggtcgtgatc cagcagggct
     4501 cgggcctgcc cggcctggac gcggccaacg cccaggtgcg gctcctcgcc gacggcacgg
     4561 tcatcctcct ctcgggcggc gccgacctcg gcaccggcct ggacacggtg acggccaagg
     4621 ccgtcgcgga gatcctgggc ctcgagatgg ccgacgtggc cgtggtctcc ggcgacaccg
     4681 acgcgacgcc cttcgacaag ggggcctacg cctcctcggg cacctacttc tcgggcaacg
     4741 cggccctcct ggccgcgaag gacctcaggg acaaggcgct cgcggtcgcc gcgggcatcc
     4801 tgggcgagcc cgtcgcggac ctccggctcg cgcaccgcgg catggtcaag ggcaagagcg
     4861 gggagataag cctcgcgaag ctcgcgcaca tggcgaccca gggcgagggc cacggcgagc
     4921 tctgcggctc ggcctgcttc aagaccgacc acgcggcctt cccctacggg gcccacttcg
     4981 tcgaggcggc ggtcaacgcg cgctcaggcg aggtgagggt gcggcgctac cacgcctacc
     5041 aggactgcgg cacgcccatc aatcccgccc tcgccaaggg gcagatcttc ggcggctgcc
     5101 tcaaggccat aggccactcc ctctacgagg agatggtcta cgacgaaaag ggccgctgcc
     5161 tgaacccgcg cttcctggac tacaagatcc cctcgatcta cgaggtgccg gaggactacg
     5221 tcgtggagct cgtgcccgtg gaggacgagg tcggcccctt cggcggcaag tccgtctccg
     5281 agatcgcgct caacggggcg gcgcccgcca tagcgatcgc gatccacgac gccgtcggcg
     5341 cctggtgccg cgagtggccc tttacgcccg agagggt
//
LOCUS       DAOOSJ010000188         5352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9575_length_5352_cov_5.440060, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000188 DAOOSJ010000000
VERSION     DAOOSJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9575_length_5352_cov_5.440060"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLG14_09355"
     CDS             complement(<1..775)
                     /locus_tag="PLG14_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49375.1"
                     /translation="MAGRSATQLVDIRLVEERRIEESYFQDYAKVHERLERIFEKTPK
                     PLPPQEAERAAEALLAWLSGQAEKSVPVERLTQFLRSGLGHICVELAFVPNYPPELVA
                     ARAYDELRRRGWTEAVFDPLEAAEAAKRLAPAEKKAPAARAAAPKAAAAPKNRVSKDP
                     SGGTAAAKSGSKKEDRRMSSIEETGVAPVAPESAEKKAPEAAKKPAPKKAAAPKKPAA
                     KKAAPAKKAVAKKPVAKKAAPAKKAVAKKPVAKKAAPAKK"
     gene            969..1973
                     /locus_tag="PLG14_09360"
     CDS             969..1973
                     /locus_tag="PLG14_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOX49376.1"
                     /translation="MLKFQKQPLMRRVLLALAPIGLHSVWLYGARALGLAAAVFAAGV
                     LAEWLVERKRSGKVSEAVLVTSALYALALPPRTPVWMALVGVAVAVFLAKGVYGGFGR
                     NVFNPAVAGRLFVYISFATTLNASYSAPGGFGAGAVDVLASATPLARMRAGQEVGLWD
                     LLLGNRAGAVGEGMVLLVLAAAVYLLVTKTASWRIMLATAASAAALNAALWFGGVRGA
                     LPMESLLAGSFLFVAVFMATDPVSAPKKKGSQLAYGALIGSAVVAIRTFSAFPEGTSF
                     AILLGNSFASFFDEIASRSQAAKAARVGGKEAAGPAGAGTASAAPGEAAPVAGKGGSA
                     "
     gene            1970..2575
                     /locus_tag="PLG14_09365"
     CDS             1970..2575
                     /locus_tag="PLG14_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOX49377.1"
                     /translation="MKKDSIAYVVAFTFVACAAFIAPLALANEGTKGRVEANRLFASR
                     SAVLRAFGVAYEGQADAEAKYASLVAELPGGRGWRAELDGASYLAVKRSGAGLWGTIT
                     MVVAADARGERLRGLEILDQQETPGLGGRIDEAWFKEQFRGERLGPAGVVVDQAGTGK
                     GDPDRENGRVDAVTGASRTSGFVQAIVNAAVAEIRAAGGSK"
     gene            2572..3228
                     /locus_tag="PLG14_09370"
     CDS             2572..3228
                     /locus_tag="PLG14_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOX49378.1"
                     /translation="MKGKSAKILKDGLWANNPIFVQVLGICSTLAVTNNVRNTLIMAA
                     GVTFATALGSLTLSAIKGLIPRKVRMIVQVLVLSFYVILVDIVLRAYAPAVSKQLGPY
                     VGLIITNCILMGRAEAFAAANPPWPSFVDGLANGLGYGLVLLCIAVVREFLGFGSVLG
                     IRLVGDWFTPWTIMIMAPSAFFLVALALWGAKSYNARAEARRKAASAAARPAPSGAAK
                     "
     gene            3241..3840
                     /locus_tag="PLG14_09375"
     CDS             3241..3840
                     /locus_tag="PLG14_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOX49379.1"
                     /translation="MPDAPAISPLVLFLASVLTSNILLSNFLGTCSFISISKDWKSSF
                     GLGLAVTLVIGLCAAICWAVLYLVVIPLGIEYLSFVVFIIVIAAVVQVLEMVIDRFSP
                     ALYLSLGIFLPLITVNCAVLGAVLFMQIRKYDFVQSVAFGFGSGAGWWLAIMLLAAIR
                     KRIENNPVPAGLKGTGITLITIGFMAMAFIGFSGMIAVQ"
     gene            3852..4970
                     /locus_tag="PLG14_09380"
     CDS             3852..4970
                     /locus_tag="PLG14_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOX49380.1"
                     /translation="MSPILLGPLVVAGLSAALALAIAVLDRFLNDYGVLKISVNGGKK
                     ELSVKGGSPLLGTLAAEGIFVPSACGGRGSCGACKCKVVTDVGPHLPTETPYLNPEEM
                     SRNVRLACQVKVRKDLEIELPEELFRVKKFAARVERIRDLTYDIKEVLFALEGQEIEF
                     EPGQYVQLVVPPYDKLPESVQRAYSMSSRPGDPRHVELLVRLVPGGIATTYVHKHLKE
                     GDRAELVGPFGDFGVRDTPATMLCVAGGSGMAPFKSILYHMKETGAWPEKEIWYFFGA
                     RTTKDMFYLEELRELAAAWPRFHFVAALSEPRPEENWQGDSGLITDVLDRYIREKIDP
                     AKGFEGYLCGSPGMINACVAVMKKNGVGEDKIYFDKFA"
     gene            4982..>5352
                     /locus_tag="PLG14_09385"
     CDS             4982..>5352
                     /locus_tag="PLG14_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49381.1"
                     /translation="MKDTIQHREAEARMRPGSITAQGFLGDEERRVAEIVEADEIEFR
                     RLGLDFASVAAELARLRDEGEKGLGEPVTLEGRWLVQTGDARGKLPCPYEDGIFHKNS
                     IWVRRLDSGEALVFSDLSIHLL"
BASE COUNT          726 a         1967 c         1747 g          912 t
ORIGIN      
        1 gcttcttcgc gggggcggcc ttcttggcga ccggcttctt cgcgacggcc ttcttcgcgg
       61 gggcggcctt cttggcgacc ggcttcttcg cgaccgcctt cttggcgggg gcggccttct
      121 tggcggccgg cttcttcgga gccgcggcct tcttgggggc gggcttcttc gcggcctcgg
      181 gcgccttctt ctcggcggac tcgggggcga cgggcgccac gccggtttct tcgatgctgc
      241 tcattctacg atcctccttc ttacttccgc tcttcgcggc ggcggtgccg cccgacgggt
      301 ccttggatac gcgattcttc ggcgctgcgg cggccttggg ggccgcggcg cgcgccgctg
      361 gggccttctt ctcggcggga gccaggcgct tcgccgcctc ggccgcctca aggggatcga
      421 atacggcttc ggtccagcct cggcggcgga gctcgtcgta ggcgcgggcc gccacgagct
      481 cggggggata gttggggacg aaggcgagct cgacgcagat gtgcccgagg ccggagcgca
      541 ggaactgcgt gagccgctcc acggggacgc tcttctccgc ttgcccggag agccaggcga
      601 ggagggcttc cgcggcccgc tccgcctcct gcggaggaag gggcttcggc gtcttctcga
      661 agatcctctc gaggcgctcg tggaccttcg cgtagtcctg gaaatatgat tcctcgatgc
      721 ggcgctcctc gacgagccgt atatcgacga gctgcgtcgc gcttctaccg gccatctcgt
      781 aatccccctt tcaataatcg gctgatttcc tcttaattgt atatctactc cgcgcgaacg
      841 caagcataat ctctctcttc ttcgctccgc taaccttctt tcgcttgcgc gggcccccgc
      901 ggccgctata ctagaggtgc ggccgcccga aaggcggccc tgcgaagcag cggaaaagga
      961 gtcgagccgt gctcaagttc cagaaacagc cgctcatgcg ccgcgtcctc ctcgccctgg
     1021 cgccgatcgg gctccactcg gtctggctct acggggcaag ggccctgggc ctcgccgcgg
     1081 cggtcttcgc cgccggcgtc ctcgcggaat ggctcgtcga gcgcaagcgc tcgggcaagg
     1141 tgagcgaggc cgtcctcgtg acctccgccc tctacgccct ggccctcccg cccaggaccc
     1201 cggtctggat ggccctggtc ggcgtcgcgg tcgcggtctt cctcgcgaag ggcgtctacg
     1261 gcggcttcgg ccgcaacgtc ttcaaccccg cggtcgcggg ccgcctcttc gtgtacatct
     1321 ccttcgcgac cacgctgaac gcctcctact ccgcgcccgg cggcttcggc gcgggggcgg
     1381 tcgacgtcct ggcctccgcg acccccctcg cgcggatgcg cgcgggccag gaggtcggcc
     1441 tctgggacct ccttctgggg aaccgcgccg gagcggtcgg cgagggcatg gtcctcctcg
     1501 tcctcgcggc ggccgtctac cttctcgtca ccaagacggc gagctggcgg atcatgctcg
     1561 cgaccgccgc ctccgccgcc gccctcaacg cggccctctg gttcggcggc gtccgcggcg
     1621 ccctccccat ggagagcctc ctcgcgggct ccttcctctt cgtcgccgtc ttcatggcca
     1681 cggatcccgt ctccgcgccg aagaagaagg gctcccagct cgcctacggc gccctcatcg
     1741 gctccgccgt cgtggcgatc aggaccttct ccgccttccc cgagggcacg agcttcgcga
     1801 tcctcctcgg gaacagcttc gcgagcttct tcgacgagat cgcctcgcgc tcgcaggccg
     1861 ccaaggccgc gcgcgtcggc ggcaaggagg cggcgggccc ggccggcgcg gggacggcgt
     1921 ccgcggcgcc gggagaagcg gcgcccgtgg cgggcaaggg aggatcggca tgaaaaagga
     1981 ctccatcgcc tacgtggtag ccttcacctt tgtcgcctgc gcggccttca tcgcccccct
     2041 cgccctcgcg aacgagggca cgaagggccg cgtggaggcg aaccggctct tcgcgtcccg
     2101 ctccgccgtc ctgcgggcct tcggcgtcgc ctacgagggc caggccgacg cggaggcgaa
     2161 gtacgcctcc ctcgtcgccg agctccccgg gggcaggggc tggcgagccg agctcgacgg
     2221 cgcctcctac ctcgccgtga agaggtcggg ggcggggcta tggggcacga tcacgatggt
     2281 cgtggcggcc gacgcccggg gcgagcggct ccgcggcctc gagatcctgg accagcagga
     2341 gacccccggc ctgggcggcc gcatcgacga ggcctggttc aaggagcagt tccgcggcga
     2401 gcgcctcggc cccgcgggcg tggtcgtgga ccaggccggg accggcaagg gcgaccccga
     2461 ccgggagaac ggaagggtgg acgcggtgac gggcgcgagc cgcacgagcg gcttcgtgca
     2521 ggccatcgtc aacgcggccg tcgcggagat ccgcgcggcg ggaggctcga aatgaagggc
     2581 aagagcgcga agatcctgaa ggacgggctc tgggcgaaca atcccatctt cgtccaggtc
     2641 ctgggcatct gctcgaccct cgcggtcacg aacaatgtcc ggaacaccct catcatggcg
     2701 gccggcgtca ccttcgccac cgccctgggg agcctcaccc tctccgccat caagggcctc
     2761 atcccccgca aggtcaggat gatcgtccag gtcctggtcc tctccttcta cgtcatcctc
     2821 gtggacatcg tcttgcgggc ctacgcgccc gcggtctcca agcagctcgg gccctacgtc
     2881 ggcctcatca tcacgaactg catcctcatg ggccgggccg aggccttcgc ggccgcgaac
     2941 ccgccttggc cctccttcgt ggacggcctg gcgaacggcc tgggctacgg cctcgtcctc
     3001 ctctgcatcg ccgtggtccg cgagttcctg ggcttcggct ccgtcctggg gatcaggctg
     3061 gtcggggact ggttcacgcc ctggaccatc atgatcatgg cgcccagcgc cttcttcctc
     3121 gtggccctgg ccctctgggg ggccaagtcg tacaacgccc gggcggaggc ccggcgaaag
     3181 gccgcctccg cggccgccag gcccgcgccc tcaggcgcgg ccaagtaagg aggaagcgcc
     3241 atgcccgacg cccccgccat cagccccctc gtcctcttcc tcgcctcggt cctgacgagc
     3301 aacatcctcc tctccaactt cctcgggacc tgctccttca tctccatctc caaggactgg
     3361 aagagctcct tcggcctcgg cctcgcggtt accctggtca tcggcctctg cgccgcgatc
     3421 tgctgggccg tcctctacct cgtcgtcatc cccctcggga tcgagtacct ctccttcgtc
     3481 gtcttcatca tcgtgatcgc cgccgtggtc caggtcctgg agatggtgat cgaccgcttc
     3541 tccccggccc tctacctctc cctcgggatc ttcctccccc tcatcacggt gaactgcgcc
     3601 gtcctgggcg cggtgctctt catgcagatc cggaagtacg acttcgtcca gtccgtcgcc
     3661 ttcggcttcg gctcgggggc gggctggtgg ctcgccatca tgctcctggc cgccatccgg
     3721 aagcggatcg agaacaatcc cgtgccggcc ggcctcaagg gcaccggcat caccctgatc
     3781 accatcggct tcatggcgat ggccttcatc ggcttctccg gcatgatagc cgtccagtag
     3841 aggaggagag cgtgagcccc atcctgcttg gccccctcgt cgtcgcgggc ctctccgcgg
     3901 ccctggccct ggccatcgcg gtcctggacc gcttcctcaa cgactacggc gtcctcaaga
     3961 tctccgtcaa cggcgggaag aaggagctgt ccgtgaaggg cggctccccc ctcctgggca
     4021 ccctggccgc ggagggcatc ttcgtcccct ccgcctgcgg cggccgcggc tcctgtggcg
     4081 cctgcaagtg caaggtcgtc acggacgtcg ggccccacct gcccacggag acgccctacc
     4141 tcaatcccga ggagatgtcg cggaacgtgc gcctggcctg ccaggtgaag gtccggaagg
     4201 acctggagat cgagctcccc gaggagctct tccgcgtcaa gaagttcgcc gcgagggtgg
     4261 agcggatccg cgacctcacc tacgacatca aggaggtcct cttcgccctg gagggccagg
     4321 agatcgagtt cgagccgggc cagtacgtcc agctcgtcgt gcccccctac gacaagctcc
     4381 cggagagcgt ccagcgcgcc tactcgatgt cctcgcgccc gggcgacccg cgccacgtgg
     4441 agctcctcgt ccgcctggtc cccggcggca tcgcgacgac ctacgtccac aagcatttaa
     4501 aggaagggga ccgcgcggag ctcgtcgggc ccttcggcga cttcggcgtc cgcgacaccc
     4561 ccgccacgat gctctgcgtc gcgggcggct cgggcatggc ccccttcaag agcatcctct
     4621 accacatgaa ggagaccggg gcctggccgg agaaggagat ctggtacttc ttcggcgcca
     4681 ggaccacgaa ggacatgttc tacctcgagg agctgcggga gctcgccgcc gcctggcccc
     4741 gcttccactt cgtcgcggcc ctctcggagc cccgccccga ggagaactgg cagggcgact
     4801 ccggcctcat caccgacgtc ctggaccgct acatccgcga gaagatcgac cccgcgaagg
     4861 gcttcgaggg ctacctctgc ggctcgccgg gcatgatcaa cgcctgcgtc gccgtcatga
     4921 agaagaacgg cgtcggcgag gacaagatct acttcgacaa attcgcctaa gggaggccgc
     4981 gatgaaggac acgatccagc acagggaagc ggaggcgcgc atgcgccccg gctccatcac
     5041 cgcccagggc ttcctcggcg acgaggagag gcgggtcgcg gagatcgtcg aggccgacga
     5101 gatcgaattc cgccgcctgg gcctggactt cgcctccgtc gcggcggagc tcgcgcggct
     5161 ccgcgacgag ggcgagaagg gcctgggcga gcccgtgacc ctggagggcc gctggctcgt
     5221 ccagacgggc gacgcgaggg gaaagctccc ctgcccctac gaggacggca tcttccacaa
     5281 gaactcgatc tgggtgcgcc gcctggactc gggcgaggcc ctggtcttct ccgacctctc
     5341 catccacctc ct
//
LOCUS       DAOOSJ010000189         5317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9657_length_5317_cov_5.151463, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000189 DAOOSJ010000000
VERSION     DAOOSJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_5317_cov_5.151463"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLG14_09390"
     CDS             complement(<1..761)
                     /locus_tag="PLG14_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX49382.1"
                     /translation="MKAIELAKAYDPKSFEDRIYEAWKAEGLFQPAGVDGRKPFVVVI
                     PPPNVTGVLHMGHGLNHSLQDISVRYARMCGRPTLWLPGTDHAGIATQNVVEKRLRKE
                     GKNRHELGREKFVEETWKVANEHKSTIRRQIAKIGDSVDWSRERFTLDEGLSKAVREV
                     FVTLYERGLVYKGKYLVNWCCSCGTALADDEVEKADEPGKMWHIWYELEDGPAPGFPE
                     GRIEIATTRPETLLGDTAVAANPEDERYRGLVGKRV"
     gene            948..2342
                     /locus_tag="PLG14_09395"
     CDS             948..2342
                     /locus_tag="PLG14_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX49383.1"
                     /translation="MTYRTERRLVGAGTTAAAALLFLLAPIVASRGIKLDAALSLGAA
                     LFLGLYVFREAVYGTLDRIIYRRRFRKPETRILLDFAERVGASFTILDLIEAIRETLE
                     RPADMGAILVRSNTWELIYQSPSAAATDPKVLEALEKNFRDWSEGFSFINADYSIASS
                     NEGARGFFVYAKGFHFFVLTRLCSIIELEAFRTLYGELSIYFDRVITISDLFEVASLS
                     KEWQLVAETQRSFLPKALPEVKKLELAVAYRPLVNVSGDYYDAIKVDESRTLLLIGDV
                     SGKGLAAALIMGIIVNTIRVAEDKSDLAALVRAVDAAVRDMGFDDKYTVLFLGLVDTA
                     KKTLRYVNAAMADPIIIAQTALGPKLRRLEPTMGLVGLVPIDGEIKVEEMPLRTDEVI
                     LLASDGVTEVADASGARLGETELFERTLLQAAKLPPEDFVGSVTSLIYSFVGDAELKD
                     DVTILAAKAGRLWD"
     gene            2333..4468
                     /locus_tag="PLG14_09400"
     CDS             2333..4468
                     /locus_tag="PLG14_09400"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOX49384.1"
                     /translation="MGLNFISFLAALGIAFAGLSIQRRGSRNAAARLLLVVDLFSCAY
                     AVLHGLGTAPGGADHAALLRAAFACLVVAAGFLLAYGINFPSRRGRWSWPLSALAVAA
                     SAAAAYFVATSLDFIAIVRVLPEGVLVSTGRLFYPLVDLVAAVGLLAAVLQGWRSFAS
                     ADRITRQRSALALLAVLLSAGGTWALIRLFPERSGFRRSFVLLPLAPLASAAVSAYAY
                     SLARIFDWRENGRRLLSYLILGLLFGLPASAAIAFLAYLGRVYPIVPVLGAPIVFVTA
                     FRGAKSFSERSLSRLGSRREYREELESALAHIDLSRGRDAVLSELHEELSEHLGFKDL
                     AVLVEDDRGFLKTVYAPSGGQASLGRDSALREHLERTVHTVLLRSEALASPAHERLRD
                     ELLELFDALKAEALIFAKEGRRVIGAFAIGARSSGADFTDYDYDTFRSIYGKLFVFAF
                     YLKNVARESILYTVDRELALSDQIIRFALEKVDPIEDPAVDAAWAMRSTRRLGGDFVD
                     FVRLTKDRSFFVLGDVSGKGLSASMNMLVLKSMIRTFLRAEKDFTSLVQRVNLFIKEN
                     LPKGSFFAGVFGYFDFAKGAVYFINCGVPAILLYSPSFDAFIEVQGEGRILGFVRDIK
                     PYLKPRKLSLPAGSALLVSTDGVLDGENIRGERFGKDRLRRSAHERLGKSARELADGV
                     LDDLLAFTDKRQEDDLTLLAMKFMPRSAK"
     gene            4465..4791
                     /locus_tag="PLG14_09405"
     CDS             4465..4791
                     /locus_tag="PLG14_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX49385.1"
                     /translation="MTQLDIVERRDGDRLVVAVAGPVNSYTYTDFQEKVSKALAAHPL
                     VVVDMEKVTTLSSAGIGVLMAALDEAEAEGKRIVVLRPSEIARLALDSTGFGERFPVA
                     QNIRDL"
     gene            4792..5268
                     /locus_tag="PLG14_09410"
     CDS             4792..5268
                     /locus_tag="PLG14_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49386.1"
                     /translation="MAEAPNAAGEKAPPRARAAEATARIPALPAGVFAFERFVDGLAF
                     AGAGERHRLKLAGSEIFDNIMHHATPLEDGTVIVRAARRGGSLFLAFHFKSRTFAAYA
                     ASCADEGADAEGPTFDRERRRWRGIGLRMCRNLALSVRFRSGGRLDRIYLRFSPSD"
BASE COUNT          735 a         2029 c         1654 g          899 t
ORIGIN      
        1 acgcgcttcc cgacgaggcc cctgtagcgc tcgtcctcgg ggttggcggc gacggcggtg
       61 tcgcccagga gggtctccgg ccgcgtggtc gcgatctcga tgcggccctc ggggaagccg
      121 ggcgcgggcc cgtcctcgag ctcgtaccag atgtgccaca tcttcccggg ctcgtcggcc
      181 ttctccacct cgtcgtcggc cagggccgtg ccgcaggagc agcaccagtt gaccaggtac
      241 ttgcccttgt agacgaggcc ccgctcgtag agggtgacga agacttcgcg gacggccttg
      301 gacaggccct cgtccagggt gaagcgctcg cggctccagt ccacggagtc gcctatcttg
      361 gcgatctgcc tgcggatggt cgacttgtgc tcgttggcga ccttccaggt ctcctccacg
      421 aacttctccc ggcccagctc gtggcggttc ttgccctcct tgcggaggcg cttctcgacg
      481 acgttctgcg tcgcgatgcc cgcgtggtcg gtgccgggga gccagagggt cggccggccg
      541 cacatgcggg cgtagcggac cgagatgtcc tggagggaat ggttgaggcc gtggcccatg
      601 tggaggaccc ccgtgacgtt cgggggcggg atgacgacga cgaagggctt ccttccgtcg
      661 acgcccgcgg gctggaagag gccctcggcc ttccacgcct cgtagatgcg gtcctcgaag
      721 gatttgggat cgtaggcctt ggccagctcg atcgctttca tagggaagcc tccatttcca
      781 gtcgcgcgcg ctcctttttc gacgcgcgcc gcttctggtc agggtaacag aaaggcggct
      841 ggcatggaaa ggggcggcgc gggcgccgag aggcgccgaa ttcggttgac tgggggccca
      901 ggggggtcta aaagtatcca gatactccaa ggagggaatt cttcgacatg acgtacagga
      961 cagaacggag gctggtcggg gcgggaacca cggccgccgc cgccctcctc ttcctcctcg
     1021 cgcccatcgt ggcctcgcgg gggatcaagc tcgacgccgc cctgtccctc ggagccgccc
     1081 tcttcctggg cctctacgtc ttccgcgagg cggtctacgg gaccctggac cggatcatct
     1141 accgccgccg cttcaggaag ccggagacga ggatcctcct cgacttcgcc gagcgggtcg
     1201 gcgcctcctt cacgatcctg gacctcatcg aggcgatccg cgagaccctg gagcggccgg
     1261 ccgacatggg cgccatcctg gtgcgctcca acacctggga gctcatctac cagagcccct
     1321 cggccgccgc gaccgacccc aaggtcctcg aggccctgga gaagaacttc cgcgactggt
     1381 cggagggctt cagcttcatc aacgccgact actcgatcgc gagctccaac gagggggcgc
     1441 ggggcttctt cgtctacgcg aagggcttcc acttcttcgt cctcacccgc ctctgctcca
     1501 tcatcgagct cgaggccttc aggaccctct acggcgagct ctccatctac ttcgaccggg
     1561 tcatcacgat ctccgacctc ttcgaggtcg cctccctgtc caaggaatgg cagctcgtcg
     1621 ccgagaccca gcgctccttc ctcccgaagg ccctgcccga ggtcaagaag ctcgagctcg
     1681 ccgtggccta ccgccccctc gtcaacgtct cgggggacta ctacgacgcc atcaaggtcg
     1741 acgagagcag gaccctcctc ctcatcggcg acgtctcggg caagggcctc gcggccgccc
     1801 tgatcatggg catcatcgtc aacacgatcc gcgtcgcgga ggacaagtcg gacctcgcgg
     1861 ccctggtccg cgccgtggac gccgccgtcc gcgacatggg cttcgacgac aagtacaccg
     1921 tgctcttcct gggcctcgtc gacacggcca agaagaccct gcgctacgtg aacgccgcca
     1981 tggccgaccc catcatcatc gcccagacgg ccctcgggcc caagctcagg cggctcgagc
     2041 cgacgatggg cctcgtgggc ctcgtgccca tcgacggcga gatcaaggtc gaggagatgc
     2101 cgctcaggac ggacgaggtc atcctcctcg cctccgacgg cgtcaccgag gtcgccgacg
     2161 cctcgggcgc gcgcctgggc gagaccgagc tcttcgagcg gaccctgctc caggcggcca
     2221 agctcccgcc ggaggacttc gtgggctccg tgacgagcct catctacagc ttcgtgggag
     2281 acgccgagct caaggacgac gtcaccatcc tcgcggcgaa ggcgggcagg ctatgggact
     2341 gaacttcatc tccttcctcg cggccctggg gatcgccttc gcgggcctct cgatccagag
     2401 gcgcggctcg cgcaacgcgg ccgcccgcct cctccttgtc gtcgacctct tctcctgcgc
     2461 ctacgccgtc ctccacggcc tggggacggc cccgggcggg gccgaccacg cagccctcct
     2521 ccgcgcggcc ttcgcctgcc tcgtggtcgc ggccggcttc ctcctcgcct acggcatcaa
     2581 cttcccgtcc aggcgcggcc gctggtcctg gcccctctcc gccctcgcgg tcgccgcctc
     2641 ggccgccgcg gcgtatttcg tcgcgacgag cctggacttc atcgccatcg tccgcgtcct
     2701 cccggagggc gtcctcgtct ccacgggccg gctcttctac cccctcgtcg acctggtcgc
     2761 cgccgtcggc ctcctcgcgg ccgtcctcca gggctggcgg tccttcgcga gcgccgaccg
     2821 catcacgcgc cagcgctccg ccctggccct cctcgccgtc ctcctctccg cgggcgggac
     2881 ctgggccctc atccgcctct tccccgagcg ctcgggcttc cgccggagct tcgtcctcct
     2941 ccccctcgcg cccctggcct ccgcggcggt ctccgcctac gcctactccc tggccaggat
     3001 cttcgactgg agggagaacg gccgccgcct cctctcctac ctgatcctgg gcctcctctt
     3061 cggcctgccc gcgagcgccg cgatcgcctt cctcgcctac ctgggccggg tctacccgat
     3121 cgtgccggtc ctcggcgcgc cgatcgtctt cgtgacggcc ttccgcgggg ccaagagctt
     3181 ctccgagcgc tccctctccc gcctgggctc ccgccgggag taccgcgagg agctggagag
     3241 cgccctggcc cacatcgacc tgtcgcgggg ccgcgacgcg gtgctctccg agctccacga
     3301 ggagctctcc gagcacctgg gcttcaagga cctggccgtc ctcgtggaag acgaccgggg
     3361 cttcctcaag accgtctacg cgccctccgg cgggcaggcc tccctcggcc gcgactccgc
     3421 gctcagggag cacctggagc ggacggtcca caccgtcctc ctccgctccg aggccctggc
     3481 ctcccccgcc cacgagcgcc tccgggacga gctcctcgag ctcttcgacg ccctcaaggc
     3541 cgaggcgctg atcttcgcca aggagggccg gcgcgtcatc ggcgccttcg cgatcggcgc
     3601 gcggagctcg ggcgccgact tcacggacta cgactacgac accttcaggt cgatctacgg
     3661 caagctcttc gtcttcgcct tctacctgaa gaacgtcgcg cgcgagtcca tcctctacac
     3721 ggtcgaccgc gagctcgccc tctccgacca gatcatccgc ttcgccctgg agaaggtcga
     3781 ccccatagag gaccccgccg tcgacgccgc ctgggccatg cgctccacgc ggcgcctggg
     3841 cggcgacttc gtcgacttcg tgcggctcac gaaggaccgc tccttcttcg tcctgggcga
     3901 cgtctccggg aagggcctgt cggcaagcat gaacatgctc gtcctcaagt ccatgatcag
     3961 gaccttcctc cgggcggaga aggacttcac gagcctcgtg cagagggtca acctcttcat
     4021 caaggagaac ctgcccaagg gctccttctt cgcgggcgtc ttcggctact tcgacttcgc
     4081 caagggcgcc gtctacttca tcaactgcgg cgtgccggcc atcctcctct actcgccgag
     4141 cttcgacgcc ttcatcgagg tccagggcga gggcaggatc ctcggcttcg tgcgggacat
     4201 caagccctac ctgaagccgc ggaagctcag cctgcccgcc ggctcggccc tcctcgtctc
     4261 cacggacggc gtcctggacg gcgagaacat ccgcggcgag cgcttcggca aggaccgcct
     4321 gcgccgctcc gcccacgaga ggctcgggaa gagcgcccgc gagctggcgg acggcgtcct
     4381 cgacgacctc ctcgccttca ccgacaagcg ccaggaggac gacctcaccc tcctcgcgat
     4441 gaaattcatg ccaaggagcg ccaaatgacc caactcgaca tcgtggagcg ccgggacggc
     4501 gacaggctcg tggtcgcggt cgcgggcccc gtgaacagct acacctacac ggacttccag
     4561 gagaaggtga gcaaggccct cgccgcccat cccctggtcg tcgtcgacat ggagaaggtg
     4621 accaccctct cctccgccgg gatcggcgtc ctcatggccg ccttggacga ggccgaggcc
     4681 gaggggaagc gcatcgtcgt cctcaggccc tcggagatcg ccaggctcgc cctggattcc
     4741 acgggcttcg gggagcgctt tcccgtggcg cagaacatcc gggacctgta gatggccgaa
     4801 gctcccaacg cggcggggga gaaggctccc ccccgcgcgc gggctgccga ggcgaccgcc
     4861 cgcattccgg ccctgcccgc gggcgtcttc gccttcgagc gcttcgtcga cggcctcgcc
     4921 ttcgccggtg ccggggagcg ccaccgcctc aagctcgcgg gctcggagat cttcgacaac
     4981 atcatgcacc acgccacccc cctggaggac gggaccgtca tcgtccgggc ggccaggcgc
     5041 ggcggctccc tcttcctcgc cttccacttc aagtcgcgga ccttcgcggc ctacgccgcc
     5101 tcctgcgccg acgagggcgc cgacgccgag ggcccgacct tcgaccggga gcggcggcgc
     5161 tggcgcggca taggcctccg gatgtgccgg aacctcgccc tctccgtgcg cttccgctcg
     5221 ggcggccgcc tggaccgcat ctacctccgc ttttccccct cggactaaca tcctcccttt
     5281 ctcattccgt cccccttccg ccgtgtcggg cgacgcc
//
LOCUS       DAOOSJ010000190         5292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9723_length_5292_cov_5.157151, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000190 DAOOSJ010000000
VERSION     DAOOSJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9723_length_5292_cov_5.157151"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1322)
                     /locus_tag="PLG14_09415"
     CDS             complement(153..1322)
                     /locus_tag="PLG14_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49387.1"
                     /translation="MKRILSLAAVLLALAAGSAFSGEEAEVYRRIYLEAEGLSQKYAA
                     AQNLVRLDDRSTAPVLAEALEELLRTQNSYKDASEKELYARTVRMLAAALGDYKHEES
                     AAFLWDVVEEVSEPLAKSEALVALGKMRALAYAERISLMLRDLNMGPTADRDSGEKVA
                     YGCVVALEKLKDPRGFPPVFYAADGWYSQRVRQQAERALPNILADPTDAIKELLRLES
                     PERKVLALRLNASSRASVERKTEAAALALALGHEKAARDKAEARVMADLRKLALRSLV
                     AYKARGPEAVPATAASYENGYDIEERLLALSALGANGGDAAAAALRDIILKLDREQKA
                     GVSDETRNRLAKAAIENAGASKNRTLRPALVAVASNDKWSGGIILAAQNAVKEIP"
     gene            complement(1335..2198)
                     /locus_tag="PLG14_09420"
     CDS             complement(1335..2198)
                     /locus_tag="PLG14_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YD repeat-containing protein"
                     /protein_id="HOX49388.1"
                     /translation="MQRHGFPLLAALCAALVLASCAGAPKAPAAPEKIRKEREVVVKT
                     PVTAKETAFYADGLVDEYSIYKYDAENRLLLEKAVYDAARPEPVERYAYEYAAGRLAG
                     ELSYDAEGKPRQRREYAYDASGRLASERVLDAKGLPLSSSAYAYDAAGRRVEWRALDG
                     SGLLRALTAYAYEGDRLVLVEMKDAASRRTGSIRLEYGPSGLAARKSYFAADGSLQKY
                     EAMAYEGGRLASLELRRADGSLASRSAYAYGPLGELLSRSDFDGSGALKARLAVEYLV
                     REDRKTEVYYE"
     gene            2352..3860
                     /locus_tag="PLG14_09425"
     CDS             2352..3860
                     /locus_tag="PLG14_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOX49389.1"
                     /translation="MKRQTRSKAVLLAAALAALAAAALAACASGPQAKAEPAAAPAAA
                     PAPQQAAPAPTAVEPVSTTPAAPSAAAQAPAAAPAASPAPTAQPPVSQAPAAPKAAAP
                     APAAPQAAAKPAQPKPATPAPAQPLPPARALGEIVYAEGEVSIRRGDKTLTGDAADIG
                     GRVLAYDLLLTGAKSRAEVDLGSSASGGALVKVGERTAFYFDTQELDDGTRRSLIRLL
                     SGSLALKVEKLGSGQLTVASSGAVLGVRGTTFAVDASPDGSLLVSCAEGRVACRGADG
                     AEFIARPGTAVEAVGSPAASPGEGLALAPKPLPAERLSSYRASWLLDRDAALAAGGAP
                     LAASLAAELSGARPALDAAYARLEAQAGALAAWEKLAAAGKTFRLTERIEERKAVAAA
                     LLGCLKALPSYERPFYRLADLASRRDALLSGANGAPRELGGALPASSPALPGLFAAFK
                     AEREASELRLARIRRALFLFSSLSGDSPLGQFFGEKAEGLGSGGASLLDLDF"
     gene            3979..4260
                     /locus_tag="PLG14_09430"
     CDS             3979..4260
                     /locus_tag="PLG14_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOX49390.1"
                     /translation="MNDLFKALSDPTRRRILELLKAGNLSAGEIADSFDIGKPAISHH
                     LAILKAADLVEAERSGQNIIYSLNTTVFQELLAWVYGLRGAEGEPHEDR"
     gene            4247..4915
                     /locus_tag="PLG14_09435"
     CDS             4247..4915
                     /locus_tag="PLG14_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOX49391.1"
                     /translation="MRTDRFLTGFAAASFAGMAAAYFFLPESVPIHWSASGQVDRWGG
                     KANVLWMGALPLLAALLLRFLPRIDPRREAYARHAKSYDAIKLFLVPSLAAFGWIAVA
                     AALGVGVDAALLTRAVVGVLLVGTGNFLGRLKRNFFVGIKTPWALADDEVWRLTHRRG
                     GYVFVAMGLAFLVSLLLPPGPFLEAFLLASSLGGVCYACLYSYLAWRRLRGPGAKLGD
                     SGRG"
     gene            complement(5072..>5292)
                     /locus_tag="PLG14_09440"
     CDS             complement(5072..>5292)
                     /locus_tag="PLG14_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOX49392.1"
                     /translation="ALCVIETERIAIRRFDDVDEAFAAREGEGDGSLAYWRDAHRRYF
                     GAEHAALGLPFDGEAPVLCEEFGVVWPR"
BASE COUNT          655 a         1982 c         1887 g          768 t
ORIGIN      
        1 gaggtagcgg atggaggggt cgcggcggcc ctccgtgtcc tcgaagaggc aggccgcgat
       61 gccctcctcc ttgaggcggg cggcggcggc ggcgcggcgg gcggcgtagg cggagggctc
      121 gaagagtccg tcgggaccgg gctgggacgt tttcacggta tctccttcac ggcgttctgg
      181 gctgccagga tgatgccgcc cgaccacttg tcgttggagg cgacggcgac cagggcggga
      241 cggagggtgc ggttcttcga ggcgccggcg ttctcgatgg cggccttggc caggcggttc
      301 ctggtctcgt cggagacgcc ggccttctgc tcgcggtcga gcttcaggat gatgtcccgc
      361 agggccgcgg ccgcggcgtc gccgccgttc gcgccgaggg cggagagggc gaggaggcgc
      421 tcctcgatgt cgtagccgtt ctcgtaggag gcggccgtcg cggggacggc ctcgggcccg
      481 cgggccttgt aggcgacgag ggagcgcagg gcgagcttgc gcaggtcggc catcacccgg
      541 gcctcggcct tgtccctcgc ggccttctcg tggcccaggg ccagggccag ggcggcggcc
      601 tccgtcttgc gctccacgga ggcgcgggag gaggcgttga gcctgagggc caggaccttg
      661 cgctcggggg actcgagcct gaggagctcc ttgatggcgt ccgtcgggtc ggccaggatg
      721 ttcgggaggg cgcgctcggc ctgctggcgg acgcgctggg agtaccagcc gtcggccgcg
      781 tagaagaccg gcgggaagcc gcgcgggtcc ttgagcttct ccagggcgac cacgcagccg
      841 taggcgacct tctcgcccga gtcgcggtcg gcggtcgggc ccatgttgag gtcgcggagc
      901 atgagggaga tgcgctcggc gtaggccagg gcgcgcatct tgccgagggc gaccagggcc
      961 tcggacttgg ccaggggctc gcttacctct tcgacgacgt cccagaggaa ggccgcggac
     1021 tcctcgtgct tgtagtcgcc cagggcggcg gcgagcatgc ggacggtccg cgcgtagagc
     1081 tccttctcgg aggcgtcctt gtagctgttc tgcgtgcgga ggagctcctc gagcgcctcc
     1141 gcgaggacgg gggccgtgct ccggtcgtcc aggcggacca ggttctgggc ggcggcgtac
     1201 ttctgggaga ggccctcggc ctcgagatag atgcggcggt agacctccgc ctcctcgcct
     1261 gagaaggccg atccggccgc gagggcgagg aggacggcgg cgagggaaag gatgcgcttc
     1321 attcgggtgc tccgttattc gtagtagact tcggtcttcc ggtcctcgcg gaccaggtat
     1381 tcgacggcaa ggcgcgcctt gagggcgccc gagccgtcga agtcggaacg ggagaggagc
     1441 tcgccgagcg ggccgtaggc gtaggcgctc cgcgaggcga gggagccgtc ggcgcggcgc
     1501 agctcgaggg aggcgaggcg gccgccctcg taggccatgg cctcgtactt ctggagggag
     1561 ccgtcggccg cgaagtagga cttcctggcg gcgaggccgg acgggccgta ctcgagccgg
     1621 atcgagcccg tgcgcctgga ggccgcgtcc ttcatctcga cgaggacgag cctgtcgccc
     1681 tcgtaggcgt aggcggtcag ggcgcggagg aggccgctcc cgtccagggc ccgccactcg
     1741 acgcggcgcc cggcggcgtc gtaggcgtag gccgaggagg agagggggag gcccttcgcg
     1801 tccaggacgc gctcggaggc caggcggccg gaggcgtcgt aggcgtactc gcggcgctgc
     1861 ctcggctttc cctcggcgtc gtaggagagc tcgcccgcga ggcgcccggc ggcgtactcg
     1921 taggcatagc gctcgacggg ctcgggcctg gccgcgtcgt agacggcctt ctcgaggagg
     1981 aggcggttct ccgcgtcgta tttgtagatc gagtactcgt cgacgaggcc gtcggcgtag
     2041 aaggcggtct ccttcgccgt cacgggcgtc ttgacgacga cttcgcgctc cttgcggatc
     2101 ttttcgggcg cggcgggcgc cttgggggcc ccggcgcagg aggcgaggac tagggcggcg
     2161 cacagggccg ctaacaaagg gaatccgtgt cgttgcatga ggacctccgg ctggtttcct
     2221 gaaggcaggg aggcgctcaa tatagcgaaa aagccccgcc tagaaaaagc ccttcccgcg
     2281 gtgcttcaaa aaccgggccg ccccgtctat acttcgccca taatagtcgt accgagcgga
     2341 gaagccatcc catgaaacgc cagactcgat cgaaggccgt cctcctcgcc gccgccctgg
     2401 ccgccctcgc cgcggccgcc ctggccgcct gcgcctcggg cccccaggcc aaggccgagc
     2461 cggccgccgc gcctgccgcc gcgccggccc cccaacaggc cgcccccgcc cccacggcgg
     2521 tcgagcccgt ctccaccacc cccgcggctc cctccgccgc cgcccaggcc cccgccgcgg
     2581 cgcctgccgc ttcgccggcc cccacagccc agccgcccgt ctcccaggcg ccggccgccc
     2641 ccaaggcggc cgctccggcc ccggccgcgc cccaggcggc cgccaagccc gcccagccca
     2701 agcccgcgac tcccgccccg gcccagcccc tgccgcccgc ccgggccctg ggcgagatag
     2761 tctacgccga gggagaggtc tccatccgcc gcggcgacaa gaccctcacg ggagacgcgg
     2821 ccgacatcgg cggccgcgtc ctcgcctacg acctcctcct cacgggggcc aagagccggg
     2881 ccgaggtcga cctgggctcc tccgcctcgg gcggcgccct cgtcaaggtt ggcgagcgga
     2941 ccgccttcta tttcgacacg caggagctcg acgacggcac caggcggagc ctcatccgcc
     3001 tcctctccgg ctccctggcc ctcaaggtcg agaagctcgg ctcgggccag ctcaccgtcg
     3061 cctcctcggg ggccgtcctg ggcgtcaggg gcacgacctt cgccgtcgac gcctcgccgg
     3121 acggctccct cctcgtctcc tgcgccgagg gccgcgtcgc ctgccgcggg gccgacggcg
     3181 ccgagttcat cgcccgcccc ggcaccgcgg tggaggccgt cggctctccc gccgcctcgc
     3241 ccggcgaggg cctggccctg gcgcccaagc ccctccccgc cgagcgcctc tcctcctacc
     3301 gcgcctcctg gctcctggac cgcgacgccg ccctcgccgc gggcggagcc cccctcgcgg
     3361 cctccctcgc cgcggagctc tccggcgccc gccccgccct cgacgcggcc tacgcccgcc
     3421 tggaggccca agccggcgcc ctggccgcct gggagaagct ggccgccgcg ggcaagacct
     3481 tccgcctcac ggaaaggatc gaggagcgca aggccgtcgc cgccgccctc cttggctgcc
     3541 tcaaggccct cccctcctac gagcggccct tctaccgcct cgccgacctg gcctcgaggc
     3601 gcgacgccct gctttcgggc gcgaacggcg cgcctcggga gctcggcggc gcgcttccag
     3661 cctcctctcc cgccctgccc gggctgttcg cggccttcaa ggccgaacgc gaggcctccg
     3721 agctccgcct cgcgaggatc cgccgcgccc tcttcctctt ctcgagcctc tcgggcgaca
     3781 gcccccttgg ccagttcttc ggggagaagg ccgagggcct gggctcaggc ggcgcgagcc
     3841 tcctcgacct cgacttctaa ggccgtccgc cgggcggtcc tcgtccacgc gaaggctcag
     3901 ccggcgcctc ggcttgactg agccggcccg cctcgatatt ttaacatttg tcgaacactc
     3961 gaagaacgag gccgcacgat gaacgacctt ttcaaggccc tctccgaccc cacgcgcagg
     4021 cggatcctcg agctcctcaa ggcgggaaac ctgagcgcgg gagagatcgc ggattccttc
     4081 gacataggga agccggcgat cagccaccac ctggccatcc tcaaggcggc cgacctggtg
     4141 gaggccgagc ggagcggcca gaacatcatc tattcgctga acacgacggt gttccaggag
     4201 ctcctggcct gggtctacgg gctccgaggc gccgaggggg aaccgcatga ggaccgatag
     4261 attcctgacc ggcttcgccg ccgcctcctt cgccggcatg gcggcggcct atttcttcct
     4321 gcccgagtcg gtgccgatcc attggagcgc ctcgggccag gtcgaccgct ggggcggcaa
     4381 ggcgaacgtc ctctggatgg gcgccctgcc cctgctcgcg gccctcctcc tccgcttcct
     4441 tccccggatc gatccgcggc gcgaggccta cgcgcggcac gcgaagagct acgacgcgat
     4501 caagctcttc ctcgtgccga gcctcgcggc cttcggctgg atcgccgtcg cggccgccct
     4561 gggcgtcggc gtggacgcgg ccctcctcac gcgcgccgtc gtcggcgtcc tcctcgtcgg
     4621 cacgggcaat ttcctgggga ggctcaagcg caacttcttc gtcggcatca agacgccctg
     4681 ggccctcgcc gacgacgagg tctggaggct gacccaccgc cgcggcggct acgtcttcgt
     4741 cgcgatgggc ctggccttcc tcgtctcgct cctcctgccg cctggcccct tcctggaggc
     4801 cttcctcctc gcctccagcc tgggcggcgt ctgctacgcc tgcctctact cctacctcgc
     4861 ctggcgcagg ctccgcggac cgggggccaa gctcggggac tcgggccgcg gctgaggcgg
     4921 cccgccttcc cgccgccgtc ccgaaagagg cgcgcctcgg gcggccgcgg atagcgcggg
     4981 cgacgcggct cgggcaggcg ctgccccggc ggccgcggat agcgcgggcg gcgcggctcg
     5041 ggcggccgcg cggcccgcat agtccgcgcg gctaacgcgg ccacacgacg ccgaactcct
     5101 cgcagaggac gggcgcctcc ccgtcgaagg gcaggcccag ggcggcgtgc tcggcgccga
     5161 agtagcggcg gtgcgcgtcg cgccagtagg cgaggctccc gtcgccctcg ccctcccgcg
     5221 cggcgaaggc ctcgtccacg tcgtcgaagc gcctgatcgc gatccgctcc gtctcgatga
     5281 cgcagagggc cc
//
LOCUS       DAOOSJ010000191         5291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_9725_length_5291_cov_5.954545, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000191 DAOOSJ010000000
VERSION     DAOOSJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9725_length_5291_cov_5.954545"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PLG14_09445"
     CDS             complement(<1..1580)
                     /locus_tag="PLG14_09445"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOX49393.1"
                     /translation="MDTKSLDKIALSVRSLSMDAIEAAKSGHPGLPLGCAELGAFLYG
                     EAMKADPSAPAWLDRDRFVLSAGHGSMFLYSLLHLAGYGLSLDDVKRFRQLGSPCAGH
                     PEYGMVPGVETTTGPLGQGVANAVGLAIAEAMLAARFNTAKRRIFDHYTYVLAGDGCL
                     MEGVAAEASSLAGHLALGKLVVFYDSNAITIDGSTGLAFGEDVGKRYEAYGWKVLKGS
                     MYDLPGLERLLAEAKAETAKPKLVILESVIGKGAPTKQGTHGVHGAPLGPEEIAKAKA
                     GLGIPAGAQFWVAPEAYAYFEKRRAAQKQVRAAWEAEFAAWEKEEPALAAELKALLTG
                     KPVKALDLPGFAKGEKVATRNASGKALAAVAASYPGLAGGSADLTGPNVTQIPGGDFN
                     AKDRKGRMLRFGVREHGMAAIANGMALGGLTPFVATFLSFVDYLRPSLRLAALMKLPV
                     VYVLTHDSVFIGEDGPTHQPVEQLASVRAIPNVRVLRPADAEETAAAWAMAMDRRDGP
                     TLLCLSRQNLAVFEKDDP"
     gene            1679..>1883
                     /locus_tag="PLG14_09450"
     CDS             1679..>1883
                     /locus_tag="PLG14_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOX49394.1"
                     /translation="MGRLRVDGLELELLRSERRSLSLQAGPGFGLRLRAPLGLPEPEL
                     AAFVRRKRGWIEKQLKALDAAGAL"
     assembly_gap    1884..1983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1984..2568
                     /locus_tag="PLG14_09455"
     CDS             <1984..2568
                     /locus_tag="PLG14_09455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOX49395.1"
                     /translation="KRGWIEKQLKALDAAGALDSGPRYREGGLFPFWGEPARLELVPR
                     PGSTSARASREGSLLRVELPDPGDEAAVRRALGRLYLREAKLVLPPLFADCLARARVA
                     LGPLSLPAPELRLRAMRSRWGSCDPARRIVTLATALAEHPASAAEFVAMHELAHLRER
                     NHDQAFYGLLGRLCPDWRERRAYLRLRRPGLPSI"
     gene            3040..4500
                     /locus_tag="PLG14_09460"
     CDS             3040..4500
                     /locus_tag="PLG14_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49396.1"
                     /translation="MLAAAFVFVLADKLVLTTLLAQGITRYPPRLREARQRRKNLIIP
                     IFMAIMSLVFAFALSVLLFATAEEGVGSASFAAGMTLPALAYFSVVLVLLLIWNTNTA
                     LLFRSVHEQLEGLSSGERDLRARVSVGSVDEIASIAGMVNAFCDGLSEGIRGLERIYG
                     ELSAVQGRLFEGIGASSAAAGDIGESVERAIESIGKEDEALRAVLAEAQTLKLLSSDL
                     AGKARDQAERVTASSSGVEVVMRAVGGLAGEAEGARRKSAELGASVRAGEEAVRAAAE
                     AASAVATRGADLSEINRLIAAVAAKTNLLAMNAAIEAAHAGEAGTGFSVVAEEIRALA
                     ESTAEHTRRNKESLSEILGLIRGAMEAAKRAGAAFAEVRSSSEEVESVAAGLARAMGE
                     EEGRSREILGLLGETESLGEGVAETARSLDGIALSMSTRLGDAATASADARRLAEAMR
                     ERNAELGRAVGEADGLVAKTSELDGTLAAFIQSFKT"
     gene            4517..5236
                     /locus_tag="PLG14_09465"
     CDS             4517..5236
                     /locus_tag="PLG14_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX49397.1"
                     /translation="MNPKTFALSLAALALAAGGAQAAPAGKAELSYFGRSSVKIRTAS
                     GFVVYVDPYAKGDYAEPADLVLVTHGHTDHNKVSLVALKPGAAILAPKGAARGAGLQE
                     IKEGEAKSFGPVSVRALPASNKNHARSESLGYLVSFDGIVLYHAGDTSYLPEMAGFAK
                     YGIGWALLPCDDYWNMGPAEAAKCALAMKARRVVPIHSSGDGLYGAKNAEALAAAGAS
                     AGAGAFKGVLLEPGKSTLLEP"
BASE COUNT          676 a         1885 c         1892 g          738 t
ORIGIN      
        1 tcggggtcgt ccttctcgaa gacggcgagg ttctgccggg agaggcagag gagggtgggg
       61 ccgtccctgc ggtccatcgc catggcccag gcggcggccg tctcctccgc gtcggcgggg
      121 cgcaggaccc tgacgttcgg gatggcgcgg acggaggcca gctgctcgac gggctggtgg
      181 gtcgggccgt cctcgccgat gaagaccgag tcgtgggtca ggacgtagac gaccgggagc
      241 ttcatgaggg ccgcgaggcg gagcgagggc cggaggtagt cgacgaagga gaggaaggtc
      301 gcgacgaagg gcgtgagtcc gcccagggcc atgccgttcg cgatcgcggc catgccgtgc
      361 tcgcggacgc cgaagcggag catccggccc tttcggtcct tggcgttgaa gtcgccgccc
      421 gggatctggg tgacgttggg gcccgtgagg tcggcggagc cgcccgcgag gccggggtag
      481 gaggcggcga cggcggccag ggccttgccg gaggcgttcc tggtcgcgac cttctcgccc
      541 ttcgcgaagc cgggaagatc gagggccttg acgggcttgc ccgtgaggag ggccttgagc
      601 tcggcggcga gggcgggctc ctccttctcc caggcggcga attcggcctc ccaggcggcg
      661 cggacctgct tctgggcggc gcggcgcttc tcgaagtagg cgtaggcctc gggggcgacc
      721 cagaactggg cgcctgcggg gatgccgagg ccggccttgg ctttggcgat ctcctcgggg
      781 cccaggggcg cgccgtggac gccgtgggtg ccctgcttgg tcggggctcc cttgccgatg
      841 acggactcga ggatgacgag cttgggcttc gcggtctcgg ccttcgcctc cgcgaggagc
      901 cgctccaggc cggggaggtc gtacatgctc cctttgagca ccttccagcc gtaggcctcg
      961 tagcgcttgc cgacgtcctc cccgaaggcg aggcccgtgg agccgtcgat cgtgatcgcg
     1021 ttcgagtcgt agaagacgac gagcttgccc agggccaggt ggccggcgag ggaggaggcc
     1081 tccgcggcga cgccctccat gaggcagccg tcgcccgcga ggacgtaggt gtagtggtcg
     1141 aagatcctgc gcttcgccgt gttgaagcgg gcggcgagca tggcctcggc gatggccagg
     1201 ccgacggcgt tcgcgacgcc ctggcccagg gggccggtgg tggtctcgac gccggggacc
     1261 atcccgtatt cggggtggcc ggcgcagggg ctgccgagct gcctgaagcg cttcacgtcg
     1321 tccagggaga ggccgtagcc cgcgaggtgg aggagggagt agaggaacat ggagccgtgg
     1381 ccggcggaga ggacgaagcg gtcgcggtcc agccaagcgg gcgccgaggg atcagccttc
     1441 atcgcctcgc cgtagaggaa ggcgccgagc tcggcgcagc ccaggggcag gccggggtgg
     1501 ccggacttgg ccgcctcgat cgcgtccatg gagaggctcc ggacggagag ggctatcttg
     1561 tcgagggatt tcgtgtccat gcttgctcct tgctggcgat gacttcgcag gtcggtccag
     1621 gctagaatag tatacgcagg ctggccgctt gctcaaccgg aggggaaaag gagggccgat
     1681 gggaaggttg cgggtcgacg gcctcgagct cgagctcctg cgcagcgaga ggcggagcct
     1741 cagtctccag gcggggcctg gcttcggcct ccgcctccgc gcgcccctcg gccttcccga
     1801 gcccgagctc gcggccttcg tgcggaggaa gcgcggctgg atcgagaagc agctcaaggc
     1861 cctggacgcg gcgggggccc tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnaagcgcg gctggatcga gaagcagctc aaggccctgg acgcggcggg ggccctggac
     2041 tccggcccgc gctaccgcga gggcgggctg ttccccttct ggggcgagcc agcccgcctc
     2101 gagcttgtcc cgaggccggg ctccacgtcc gcccgcgcga gccgcgaggg ctccctcctc
     2161 cgcgtggagc tccccgatcc cggcgacgag gccgccgtcc gccgggccct cgggcgcctc
     2221 tacctccgcg aggcgaagct cgtcctcccc ccgctcttcg cggattgcct ggcgcgggcc
     2281 cgcgtcgcct tggggcccct ctcccttccc gcgcccgagc tccgcctccg cgcgatgcgc
     2341 tcgcgctggg ggagctgcga ccccgcgagg aggatcgtca ccctcgccac ggccctggcc
     2401 gagcatccgg cctccgcggc ggagttcgtc gccatgcacg agctcgccca cctccgcgag
     2461 cggaaccacg accaggcctt ctacggcctc cttggccggc tctgccccga ctggcgggag
     2521 cggcgcgcct acctccgcct gcgccggccg ggactccctt ccatttgacg ccgccgcctc
     2581 cggcccctat catggaggcg tagtctcgaa ggagcccgcc gcccaggcgc gggcccgcgg
     2641 aggtcccatg aagaacaagc cacgcgcctt ccgcctcatc gtggcgagcg cggccgtcgt
     2701 tttcgccctg gcctcgtcgg ccggcctcta cctggcgagg tcggccgagt cctcccccgg
     2761 gacggcctcg gccatcctgg gcgcctcggc cgccgccttc atcctcgcgg cctacgccgc
     2821 cgtcggcctc ctcggcaagc gctacggcgt caggctcgcg gctcgcgagg gcgacgagga
     2881 ggcctacgag aaggccttcg ccgagctggg cggcgtcccc ctcaaggccc tcctccgctt
     2941 cctcctcctc gacatcgcct atctcggcgc cctcgccgcc tcctcggact gccccgggcg
     3001 acgtcggggg ccatccacct cctcatcctc tccgccggca tgctcgcggc ggccttcgtc
     3061 ttcgtcctcg cggacaagct cgtcctcacg accctcctgg cccaggggat cacgcgctac
     3121 ccgcccaggc tccgcgaggc ccgccagcgc cgcaagaacc tcatcatccc catcttcatg
     3181 gccatcatgt ccctcgtctt cgccttcgcc ctctcggtcc tcctcttcgc gacggccgag
     3241 gagggggtgg gcagcgccag cttcgcggcg ggcatgaccc tgcccgccct ggcctatttc
     3301 tccgtggtcc tggtcctcct cctcatctgg aacaccaaca cggccctcct tttccgcagc
     3361 gtccacgagc agctcgaggg cctctcctca ggcgagcgcg acctgcgcgc ccgggtcagc
     3421 gtcggctcgg tcgacgagat cgcctccatc gcgggcatgg tgaacgcctt ctgcgacggc
     3481 ctctccgagg gaatccgcgg cctggagcgg atctacggcg agctctccgc ggtgcagggc
     3541 aggctcttcg agggcatcgg cgcctcctcc gcggccgcgg gggacatcgg cgagagcgtg
     3601 gagcgggcca tcgagtccat cggcaaggag gacgaggccc tcagggccgt cctcgccgag
     3661 gcccagaccc tgaagctcct ctcctccgac ctcgcgggga aggcccgcga ccaggcggag
     3721 cgggtcaccg cctccagctc gggcgtagag gtggtcatgc gcgccgtggg cggcctcgcg
     3781 ggcgaggccg agggcgcgag gcggaagtcg gccgagctcg gggcctccgt cagggcgggc
     3841 gaggaggcgg tgcgcgccgc ggcggaggcg gcgagcgccg tcgcgacccg gggcgccgac
     3901 ctctcggaga tcaaccgcct catcgccgcc gtcgccgcga agacgaacct cctcgcgatg
     3961 aacgccgcca tcgaggcggc ccacgccggc gaggcgggca cgggcttctc cgtcgtcgcg
     4021 gaggagatcc gcgccctcgc ggagtcgacg gccgagcaca cgcggcgcaa caaggagagc
     4081 ctctccgaga tcctcggcct gatccgcggc gccatggagg ccgccaagcg ggccggggcg
     4141 gccttcgccg aggtacgctc ctcctcggag gaggtcgagt cggtcgcggc cggcctggcc
     4201 cgcgcgatgg gcgaggagga ggggcggagc cgcgagatcc tcggcctcct cggggagacc
     4261 gagtccctgg gcgagggcgt cgccgagacg gcccgcagcc tcgacggcat cgccctctcc
     4321 atgtcgacca ggctcggcga cgcggcgacg gcctcggccg acgcccgccg cctggccgag
     4381 gcgatgcgcg agcgcaacgc cgagctgggc cgggccgtcg gcgaggcgga cggcctcgtg
     4441 gcgaagacct cggagctcga cgggaccctc gccgccttca tccagtcctt caagacctga
     4501 gcaaggagag tccagcttga acccgaagac cttcgcgctt tccctcgccg ccctggccct
     4561 cgcggccggc ggcgcccagg cggccccggc cggcaaggcc gagctgtcct atttcggccg
     4621 ctcctccgtc aagatcagga cggcctcggg cttcgtggtc tacgtcgatc cctacgcgaa
     4681 gggcgactac gcggagccgg ccgacctcgt cctcgtgacc cacggccaca cggaccacaa
     4741 caaggtctcc ctcgtcgcgc tgaagcccgg ggcggccatc ctcgcgccca agggcgcggc
     4801 gaggggggcc ggcctccagg agatcaagga gggcgaggcg aagagcttcg gcccggtctc
     4861 cgtgcgggcc ctgcccgcct ccaacaagaa ccacgcgcgc agcgagagcc tgggctacct
     4921 cgtctccttc gacgggattg tcctctacca cgcgggggac acctcctacc tccccgagat
     4981 ggccggcttc gccaagtacg ggataggctg ggccctcctg ccctgcgacg actactggaa
     5041 catgggaccg gcggaggcgg cgaagtgcgc cctcgccatg aaggccaggc gcgtcgtccc
     5101 catccattcg agcggggacg ggctctacgg ggccaagaac gcggaggccc tggccgcggc
     5161 gggagcctcg gccggagccg gcgccttcaa gggcgtcctc ctcgagccgg gcaagtccac
     5221 gctcctggag ccctaggcgc cgtagccctc cggcggctcg gccgcctcgt cgaggccgga
     5281 cgagccccgg c
//
LOCUS       DAOOSJ010000192         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10223_length_5097_cov_4.742364, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000192 DAOOSJ010000000
VERSION     DAOOSJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10223_length_5097_cov_4.742364"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLG14_09470"
     CDS             complement(<1..374)
                     /locus_tag="PLG14_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOX49398.1"
                     /translation="MRGSRLRCSKLLLVGLFIAAIGLAAGAGEYRIEIRETSWELEPG
                     LSTAAYTYGGSVPGVPIVARPGERLKVEFVNRLPVATNVHWHGLVVPADQDGPALRIE
                     PGASHTYEFTVGESGTYWYHPHA"
     gene            complement(528..2231)
                     /locus_tag="PLG14_09475"
     CDS             complement(528..2231)
                     /locus_tag="PLG14_09475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOX49399.1"
                     /translation="MTKFDDLPYVRPDLEAFEAEFRAALRDFGAAGSAEGQAAAFARL
                     SSLRNGFETAASMVEIRQTRNTLDPFYEAENDFMDEAKPRVEGLESELYAALAASPFR
                     PELERRLGRQLFRLAELKMKTYKPEILEDLQAENRLGSEYVKLKASARVPFEGEERNL
                     TELLAFAESPDRGMRKRATAAIAGFYRDHEAEFDRIYDELVRLRDAIARKLGFPNFVP
                     LAYARLGRSDYGAEAVAGYRRQVHATVVPLAAALRKRQAARLGLPSLEFYDEALEFPS
                     GNPEPLGGAEGIVANGLSMFAAMSKETGDFFRLLSERGLYDLETRKGKAAGGYCSYLP
                     TERVPYIFANFNGTAEDVDTFSHECGHAFQVYRSGLAARGVPDYDWPTLEACEIHSMG
                     MEFLAWPWMDSFFGKDAAKYRFRHLSAGILFLPYGVLVDEFQHWVYGNPQVSPAERKA
                     AWRGLERKYLPWRDYSGDEFLERGGFWFRQGHIFQDPFYYIDYTLAQVSAYEFWGKAG
                     ADRGAAWASYLSLCDLGGSESYLELLRRAGLRSPFAEGAVEAIMKPVREWLDAADDRG
                     M"
     gene            complement(2444..3439)
                     /locus_tag="PLG14_09480"
     CDS             complement(2444..3439)
                     /locus_tag="PLG14_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOX49400.1"
                     /translation="MAKREQGKPRFARVLAVLALWAAAAGLGAAPQAKLAWEAKGPPG
                     DPLGYVGAVAVAPDGKVWAADASGRFFIFDEGGAFLETWGSAGSGPGQFRFIVDTGQI
                     NWERDVSAEILFLPDGSFYVRDWGNNRVQRFSSARRFLGAFGSGESPGEAPTGIAVRN
                     GNELLACFAGRTDLQRFSLEGAYRGSLVLDLVDVEFVQTTSGVAVDSAGTIYVATKFR
                     GQGGVRPRGRILTFDPKGASRGLFGVQEAEFSGLSFDFFLRLGPDGRLYAADEWNQRL
                     IAFGADRKPFFVYGEAGSGPGRFNRPSSLAFGPKGELYVADLLNRRIQKFILSGL"
     gene            complement(3566..4141)
                     /locus_tag="PLG14_09485"
     CDS             complement(3566..4141)
                     /locus_tag="PLG14_09485"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOX49401.1"
                     /translation="MGKTKTGAYIPLPAVPLVLVGANVRGRPNYLPVGFVAGVNAEPA
                     VVCVGLNKAHHTTAGILESGTFSLNVPPAGLVAEADFCGLVSGREADKSRLFTTFYGG
                     LGTAPLIEECPVVCECRLVGPKVEFAMDTVFFGEITQVYVDEGLLGPGGRVDAAKARP
                     FVFCGLENRYRALGEELGSAWSAGKGLLKAL"
     gene            complement(4340..4864)
                     /locus_tag="PLG14_09490"
     CDS             complement(4340..4864)
                     /locus_tag="PLG14_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX49402.1"
                     /translation="MTEAAARGRIILLNGVSSSGKTTLARSLAGALPGFFPLCLDAFD
                     DLVASMEDRQGGRLIPVATERFFHRTLLMFSDSGVSLVADHILHDEATRSDFYETLRG
                     REVLLVGLRCPLEEAERRERERGDRPLGLARRQAAFVHRDEAYDLEMDTSRESVEACV
                     ERIRARALGPWRRL"
     gene            complement(4861..>5097)
                     /locus_tag="PLG14_09495"
     CDS             complement(4861..>5097)
                     /locus_tag="PLG14_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49403.1"
                     /translation="FLRADQTGKGLGALALARLEEDARRLGVRKLVANLSSGNLGSLV
                     FHIRNGFEEYGRLRDAGRKFGLPFDIVYLEKSLA"
BASE COUNT          791 a         1765 c         1837 g          704 t
ORIGIN      
        1 gcgtgggggt ggtaccaata ggtgccgctt tccccgaccg tgaattcgta ggtatgggag
       61 gctcccggct cgatgcgcag ggcggggccg tcctggtcgg cgggcacgac gaggccgtgc
      121 caatgcacgt tcgtggccac gggaaggcgg ttgacgaact cgaccttgag ccgctccccg
      181 ggccgcgcga cgatggggac accgggaacc gagcccccgt aggtataggc cgccgtggag
      241 agccccggct cgagctccca gctcgtctcc cggatctcga tcctgtactc cccggcgccg
      301 gccgcgaggc cgatcgccgc gatgaagagc ccgacgagga gcagcttaga acaccgaagc
      361 cttgagccgc gcatctcttc ctcccaggaa agccgccgcc cggcgggccg gggactctcc
      421 tgaggaaaaa gatactggcg cgggcggccg gccgggccgg cttcacggaa ttcgcacgga
      481 agaagcgcgg gccgatcgcc gtcccgcgcc cctaggcccc tgccacccta catcccccgg
      541 tcgtccgccg cgtccagcca ctcccggacg ggcttcatga tcgcctcgac cgcgccttcg
      601 gcgaaggggc tcctcaggcc cgcgcgccta aggagctcga ggtaggactc gctgccgccc
      661 aggtcgcaga gggagaggta ggaggcccag gcggcgccgc ggtcggcgcc cgccttgccc
      721 cagaactcgt aggcggagac ctgcgccagg gtgtagtcga tgtagtagaa ggggtcctgg
      781 aagatgtggc cctgcctgaa ccagaagccg ccgcgctcca ggaactcgtc gccggagtag
      841 tcgcgccagg gcaggtactt cctctccaag ccccgccagg cggccttccg ctcggcgggc
      901 gagacctgcg ggttcccgta gacccagtgc tggaactcgt cgaccaggac gccgtagggg
      961 aggaagagga tgccggcgga gaggtggcgg aagcggtact tcgccgcgtc cttgccgaag
     1021 aaggagtcca tccagggcca ggccaggaac tccatgccca tcgagtggat ctcgcaggcc
     1081 tccagggtcg gccagtcgta gtcggggacg ccgcgggccg cgaggccgct cctgtagacc
     1141 tggaaggcgt ggccgcactc gtgggagaag gtgtccacgt cctccgccgt gccgttgaag
     1201 ttcgcgaaga tgtagggcac ccgctccgtg gggaggtagg agcagtagcc gcccgcggcc
     1261 ttccccttcc gcgtctccag gtcgtagagg ccgcgctccg agaggagcct gaagaagtcg
     1321 cccgtctcct tggacatggc cgcgaacatg gagaggccgt tcgcgacgat gccctcggcc
     1381 ccgccaaggg gctcgggatt gccggagggg aactccaggg cctcgtcgta gaactcgagg
     1441 gagggaaggc cgagcctcgc ggcctggcgc ttcctcaagg ccgccgcgag agggacgacg
     1501 gtcgcgtgga cctggcggcg gtagccggcg acggcctcgg ctccgtagtc cgagcggccc
     1561 aggcgcgcgt aggcgagggg gacgaaattc gggaagccga gcttccgcgc gatcgcgtcg
     1621 cgcaggcgca cgagctcgtc gtagatgcgg tcgaactccg cctcgtggtc ccggtagaag
     1681 ccggctatcg cggccgtcgc ccgcttcctc atgccgcggt cgggcgactc ggcgaaggcg
     1741 aggagctcgg tgaggttccg ctcctccccc tcgaagggca cgcgggcgga ggccttgagc
     1801 ttcacgtact cgctccccag gcggttctcc gcctgcaggt cctcgaggat ctcgggcttg
     1861 taggtcttca tcttgagctc ggcgagcctg aagagctgcc gccccaggcg ccgctccagc
     1921 tcgggccgga agggcgaggc ggcgagggcg gcgtagagct cgctctcgag gccctccacc
     1981 cggggcttcg cctcgtccat gaagtcgttc tccgcctcgt agaagggatc cagggtgttc
     2041 cgcgtctggc ggatctcgac catggaggcc gccgtctcga agccgttccg caaggaagag
     2101 aggcgggcga aggccgccgc ctggccctcc gccgagccgg ccgcgccgaa gtcgcgcagg
     2161 gccgcgcgga actcggcctc gaaggcctcc aggtcgggac ggacgtaggg caggtcgtcg
     2221 aacttcgtca tgattagtcg ttcctcgctt tcgcgcctgg gcgtcccttc cggtatagca
     2281 ggcctgggcc gcggcggcga gggaaaagac gggtatgagg aaagagtccc ccgcggcggc
     2341 gagacgcggc cgccgcggcc gggcggcggc gctcgcgaag cttggcccgg cccggggcgc
     2401 ggccggatca ccgagcgccg ggcgccgcgg gagactcgcc ggcctagagt cccgagagaa
     2461 tgaatttctg gattcgccgg ttcaggaggt cggcgacata gagctctccc ttcgggccga
     2521 aagccaagga acttggacga ttgaagcggc caggccctga gcccgcttcg ccgtagacga
     2581 agaaaggctt ccggtcggcg ccgaaggcga tcaggcgctg gttccactcg tccgcggcgt
     2641 agagccgccc gtcgggccca aggcggagaa agaagtcgaa ggagaggccc gagaactcgg
     2701 cctcctggac gccgaagagc ccccgcgagg cgcccttggg atcgaaggtg aggatccgcc
     2761 cccgcggacg gacgccgccc tggccccgga atttcgtcgc gacatagata gtccccgcgg
     2821 aatcgacggc gacgccggag gtcgtctgca cgaactccac gtcgacgagg tcgaggacca
     2881 gggagcccct gtaggcgccc tccagggaaa agcgctggag gtcggtcctc cccgcgaagc
     2941 aggccaggag ctcgttcccg ttccggacgg cgatccccgt cggcgcctcg cccgggctct
     3001 cgccggagcc gaaggccccc aggaagcgcc gggccgagga gaagcgctgg acccggttgt
     3061 tgccccagtc tcggacatag aatgagccgt cgggaaggaa gaggatctcc gcgctgacgt
     3121 cgcgctccca gttgatctgg ccggtgtcca cgatgaagcg gaactgcccg ggccccgagc
     3181 ccgcggagcc ccaggtctcc aggaaggcgc caccctcgtc gaagatgaag aaacgcccgc
     3241 ttgcgtcggc ggcccagacc ttgccgtcgg gcgcgaccgc caccgcgccg acgtagccga
     3301 ggggatcgcc gggcggaccc ttggcctccc aggcgagctt ggcctgcggc gcggcgccaa
     3361 ggccggcggc cgcggcccaa agggccagga cggcgaggac gcgcgcgaag cgcggcttcc
     3421 cttgctcccg ttttgccatg gtgttcctcc ccggcctggc cggacaggcg ggcgacgcgg
     3481 agcgcgggcc gcgcggaggc acaggccgat gaggcaagta tagcgcccgg cccgctccgc
     3541 cgtcgaaccg ctggctcccg cgcggctaca aggccttcag gagtcccttg cccgcgctcc
     3601 aggccgagcc gagctcctcg cccagcgcgc ggtaccggtt ctccagtccg cagaagacga
     3661 agggcctggc cttggccgcg tccacgcggc cgcctggccc caggagcccc tcgtcgacgt
     3721 agacctgggt tatctcgccg aagaagacgg tgtccatggc gaactcgacc ttcggcccga
     3781 cgagcctgca ctcgcagacg acggggcact cctcgatgag gggcgccgtc ccgagcccgc
     3841 cgtagaaggt cgtgaacagg cgggacttgt cggcctccct ccccgagacg aggccgcaga
     3901 agtccgcctc ggcgacaagg cccgccggcg gcacgttcag gctgaaggtc ccgctctcca
     3961 ggatgcccgc cgtcgtgtgg tgcgccttgt tgaggccgac gcagacgacg gccggctcgg
     4021 cgttcacgcc cgcgacgaag cccacgggca ggtagttggg cctcccgcgg acattcgcgc
     4081 ccacaaggac gagcgggacg gccggcaggg ggatgtaggc tccggtcttg gtctttccca
     4141 tcgtgcttcc ttccgcggcg ccggccggcg ccgcggggga aggctcgcct atactaaaat
     4201 aattgtcaaa acaattttta taatcgccct gggcccgcgc ggccgcggcc gcgcgggggt
     4261 ctcgcgcgtg gccgcgcggg ctgccgcgac gacggtcgag gcgacggtca aaggggcggc
     4321 tcctgcccct cagacccgcc tagagcctgc gccaggggcc gagggcgcgc gcgcggatcc
     4381 tctcgacgca ggcctccacg gactcccgcg aggtgtccat ctccaggtcg taggcctcat
     4441 cgcggtggac gaaggccgcc tgccgcctcg cgagcccgag gggccggtcg ccccgctccc
     4501 gctcgcgccg ctcggcctcc tcaagggggc agcggaggcc gacaaggagg acctcgcggc
     4561 cgcgcagggt ctcgtagaag tcgctcctcg tcgcctcgtc gtggaggatg tggtcggcca
     4621 cgaggctcac gcccgagtcg gagaacatca gcagggtccg gtggaagaag cgctccgtgg
     4681 ccacggggat gagcctgccg ccctgccggt cctccatgga cgcgaccagg tcgtcgaaag
     4741 cgtccaggca gagggggaag aagcccggga gcgcgcccgc gaggctcctc gccagggtgg
     4801 tcttccccga gctcgagacg ccgttcaaga ggatgatcct gccccgggcc gccgcctccg
     4861 tcacgccagg ctcttctcga ggtagacgat gtcgaagggg aggccgaact tgcgccccgc
     4921 gtcccggagg cgcccgtact cctcgaagcc gttccggata tggaagacga ggctccccag
     4981 gttgccggag gagaggttgg ccacgagctt cctgacgccc agccgcctcg cgtcctcctc
     5041 gagccgcgcg agggcgaggg cgcccaggcc cttcccggtc tggtcggcgc ggaggaa
//
LOCUS       DAOOSJ010000193         5050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10353_length_5050_cov_5.173974, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000193 DAOOSJ010000000
VERSION     DAOOSJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10353_length_5050_cov_5.173974"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1016)
                     /locus_tag="PLG14_09500"
     CDS             complement(81..1016)
                     /locus_tag="PLG14_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49404.1"
                     /translation="MADAVLEARGLGKRYGSKEALRGLGFELRRGGSLALVGPDGAGK
                     TTTIRLAAGLLSPDEGEVRVFGEAPRSPGAKRRMGYLSQRFSLYGDLSVDENIAFFAE
                     IHGLSGYEARRDRLLGMTRLEPFRGRAAGRLSGGMKQKLALACALVHEPELLLLDEPT
                     TGVDPVSRREFWAILAELLASGLSLLVATPYLDEAERCAEVGLLDRGAFLAFGSPARL
                     KASLPLAVLELPAADTRRAAAALRGLPELAEVQSFGDRLALLAADEGEARRAVGAALA
                     RAGLESGGLRLAPPSLENVFMHLVGHGSQKGEPAK"
     gene            complement(1026..2006)
                     /locus_tag="PLG14_09505"
     CDS             complement(1026..2006)
                     /locus_tag="PLG14_09505"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX49405.1"
                     /translation="MRRASAIRLVPVLAAAGFLLAACAGEEAGIVASGTIEATEIRVS
                     AQVSGRVLETPAVEGATVKAGDVLARIDPEGYRLQVGQARAGAEAARAQLELLVGGAR
                     AEDILQGKAALDQAEEALRLADADAARMRGLAASGSATAKQRDDAESRLATAKAQREG
                     AAQALRKLESFARPEELRAARARLEQAEWAVRIAEKSLADCAVLAPVPGTVLERLAEA
                     GELALAGSGVATIADLGALSLTIFVPEAELGLVALGNEARVSVDSFPGRSFPGKVAFI
                     SPKAEFTPKNVQTRDERVKQVFAVRLDLGSGEGLLRPGMPADARLVPAVR"
     gene            complement(2003..2653)
                     /locus_tag="PLG14_09510"
     CDS             complement(2003..2653)
                     /locus_tag="PLG14_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49406.1"
                     /translation="MSSEREDRKREEILDKAIALFFERGIASLTMESLAARLGVSKVT
                     LYKYFPGKDQLIGSAVERRMEVVAGQLEAMDRLPAGAFIERIGLFFDVVDQFVKPAMA
                     VFFRDLASEAPWLWDRIQRFRAERVFPKLARLIEEGQAAGYVRSDLDKGLAATLLVAM
                     AEQVARPDFIYRLPIPPAEVVGAIVSILLGGILNDKGRELLGSGLAPSAARDKERT"
     gene            complement(2895..3599)
                     /gene="deoD"
                     /locus_tag="PLG14_09515"
     CDS             complement(2895..3599)
                     /gene="deoD"
                     /locus_tag="PLG14_09515"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOX49407.1"
                     /translation="MSIHIAAEKGQIAEAILLPGDPLRAQFIAENFLEEPVQFNKVRN
                     MFGFTGRYKGQRVSVMGTGMGMPSHSIYVNELIRDYGCKRLVRIGTCGALRAEIKVRD
                     LILGLSASTDSAVNKVRFQGMDYAPAPSFRLLKAAYEVALERRMPVHVGQILSSDSFY
                     TEDPDQWKLWAKFGVIGVEMEAAELYTLAAKYGVEALAILTVSDHLVTGEATTSAERE
                     TSFKAMVEVGFAAALS"
     gene            complement(3610..4725)
                     /locus_tag="PLG14_09520"
     CDS             complement(3610..4725)
                     /locus_tag="PLG14_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX49408.1"
                     /translation="MTQALHIASFNAENFYLLLDSEYGREELEALTEADYLAMNASIY
                     NPNKDRGKIAEIARIILEEDFDLVALVEVGGMETLAAFNRIYLRGAYDCFLHEENSRR
                     GIFVGALLRKGRFPGARALAMPGAFSRNLLRLDLGPEGGDLEVFALHLKSQYGEDRGL
                     ERRIKEVELLASLVRTTNCVVLGDFNGILIRGEHQFEYEPFLDLPFRDVLEAVGVPPE
                     SRRTHYHFGPRPNFAQLDYIFCTEDIEVLDAGVIEGEIPLNREQRSRLPSDHLFIRAT
                     LRPAPRRDQVRGPGAASAEPRPAVAPPATDRPAADVRPPAPLAAAPAPAAEGPSRRPA
                     SLWDRLRRILPGYASGANPGSRGPKGPGRKPRRRDNN"
BASE COUNT          730 a         1682 c         1898 g          740 t
ORIGIN      
        1 ttgggccccc gcccgccccc cgagggcgag gaggagggcg aggacggcag gccggagccc
       61 cggaatcgcg acgcggcgct tcatttcgct ggttctcctt tttgggaccc gtgcccgaca
      121 aggtgcatga acacgttctc gaggctcggc ggcgcgagcc tgaggccgcc cgattcgagg
      181 ccggcccggg ccagggccgc gccgaccgcg cgacgggcct cgccctcatc cgcggcgagg
      241 agggcgaggc ggtcgccgaa gctctggacc tccgcgagct cgggcaggcc cctgagggcg
      301 gccgcggccc tcctcgtgtc ggccgccgga agctcgagga cggcgagggg cagcgaggcc
      361 ttgagccggg cgggactgcc gaaggcgaga aaggcgccgc ggtccaggag ccccacctcg
      421 gcgcagcgct cggcctcgtc gaggtagggc gtcgcgacga ggaggctcag gcccgaggcg
      481 aggagctcgg cgaggatggc ccagaactcg cgccgggaga cgggatcgac gcccgtggtg
      541 ggctcgtcga ggaggaggag ctcgggctcg tggacgaggg cgcaggccag ggcgagcttc
      601 tgcttcatcc ctccggagag cctgcccgcg gcccggccgc ggaagggctc gagacgagtc
      661 atcccgagga ggcggtcgcg cctcgcctcg tagcccgaca ggccgtggat ctccgcgaag
      721 aaggcgatgt tctcgtcgac ggagaggtcg ccgtagaggc tgaagcgctg cgagaggtag
      781 cccatgcggc gcttcgcgcc ggggctcctg ggcgcctccc cgaagacgcg gacctccccc
      841 tcgtcgggcg acaggaggcc cgccgcgagg cgaatcgtcg tggtcttccc cgccccgtca
      901 ggcccgacca gggcgaggga gccgccgcgc cgcagctcga agccgaggcc gcgaagggcc
      961 tccttcgagc cgtagcgctt gccgaggccg cgagcctcga ggaccgcgtc agccatcgcg
     1021 acggcctagc ggacagccgg gacgaggcgt gcgtcggccg gcatgcccgg ccgcagaagg
     1081 ccttcgccgg agcccaggtc gagcctgacg gcgaagacct gcttgacccg ctcgtccctg
     1141 gtctggacgt tcttgggcgt gaactccgcc ttgggggaga tgaaggccac cttgcccgga
     1201 aaggatctgc cggggaagga gtcgacggag acgcgagcct cgttcccaag ggcgacgagg
     1261 ccgagctcgg cctcgggcac gaagatcgtg aggctcaagg cgcccaggtc ggctatcgtc
     1321 gccacgcctg agcctgcgag ggcaagctcc ccggcctctg ccaggcgctc cagcaccgtg
     1381 cccgggacag gcgcgagtac ggcgcagtcg gcgagggact tctccgcgat gcggacggcc
     1441 cattcggcct gctccaggcg ggccctggcg gcgcggagct cctcgggtcg ggcgaaggac
     1501 tcgagcttcc tgagagcctg ggcggcgccc tctctctgcg ccttcgccgt cgcgaggcgg
     1561 gactcggcgt cgtcgcgctg cttcgccgtg gcgctgcccg aggccgcgag gccccgcatg
     1621 cgcgccgcgt ccgcgtcggc gaggcggagc gcctcctcgg cctggtccag ggcggccttg
     1681 ccctggagga tgtcctcggc cctggctccg cccacaagga gctcgagctg ggccctcgcg
     1741 gcctcggccc ctgccctcgc ctggccgacc tggagcctgt agccctcggg atcgatccgg
     1801 gcgaggacgt cgccagcctt caccgtcgcg ccctcgacgg ccggcgtctc caggacgcgg
     1861 ccggagacct gggcggagac ccggatctcg gtcgcctcga tggtgccgga ggccacgatg
     1921 cccgcctcct cccccgcgca ggccgccagc aggaagccgg cggccgccag gaccgggacg
     1981 agccgaatcg ccgatgcgcg cctcatgttc gctccttgtc gcgcgccgcg gagggcgcca
     2041 agcccgaacc cagcagctcg cggcccttgt cgttcaggat tcccccgagg aggatgctga
     2101 ctatggcgcc gaccacctcc gcggggggaa taggcagtcg gtagatgaaa tcgggcctgg
     2161 ccacctgctc ggccatcgcg acgaggagag tcgccgcgag gcccttgtcg aggtctgacc
     2221 gcacgtagcc cgcggcttgg ccctcttcga tgaggcgcgc gagcttgggg aagacgcgct
     2281 cggccctgaa gcgctggatc ctgtcccaga gccagggggc ctcggaggcc aggtcgcgga
     2341 agaagacagc catggcgggt ttcacgaact ggtccacgac atcaaagaag aggccgatgc
     2401 gctcgatgaa ggctccggcg gggaggcggt ccatggcctc caattgtccc gccacgacct
     2461 ccatccgccg ctcaaccgcg gagccgatga gctggtcctt ccccggaaag tacttgtaga
     2521 gcgtgacctt ggagacgccg agtctcgcgg ccagggactc catcgtcagc gaggcgattc
     2581 ctctctcgaa gaacaaggcg atcgccttgt cgagtatttc ctcccgcttg cggtcctctc
     2641 gctccgagct catctggtcg acctccgtgt tttacttttt gaacgttcta tgttcatttc
     2701 gtaaagcctg ggcaatgtag ctcgcctgcg aataaaggtc aagcgcctcg cggcgcgatt
     2761 cgatgagcgc gtggtggggg gcatggggta gcgttaggaa gccggaggca gggggtgccg
     2821 ccgggccggc cgcgccgccc cggacaaaaa acgcccggcc tgctcggccg ggcgctttct
     2881 ctatagcttg ggtctcagga aagggcggcg gcgaagccca cctcgaccat ggccttgaag
     2941 ctcgtctcgc gctcggcgct cgtcgtggcc tcgcccgtga ccaggtggtc gctcacggtg
     3001 aggatggcga gggcctcgac gccgtacttg gcggcgaggg tgtagagctc ggcggcctcc
     3061 atctcgacgc cgatgacgcc gaacttggcc cagagcttcc actggtcggg atcctccgtg
     3121 tagaaggagt cgctggagag gatctggccg acgtggacgg gcatcctgcg ctccagggcg
     3181 acctcgtagg cggccttgag gaggcggaag gagggcgcag gcgcgtagtc catgccctgg
     3241 aagcggacct tgttgacggc ggagtcggtg gaggcggaga ggccgaggat gaggtcgcgg
     3301 accttgatct ccgcgcggag cgcgccgcag gtgccgatgc ggaccaggcg cttgcagccg
     3361 tagtcgcgga tgagctcgtt gacgtagatc gagtggctcg gcatgcccat gcccgtgccc
     3421 atcacggaca cgcgctggcc cttgtagcgg ccggtgaagc cgaacatgtt gcggaccttg
     3481 ttgaactgga cgggctcttc gaggaagttc tccgcgatga actgggcccg caaggggtcg
     3541 cccgggagga ggatggcctc ggcgatctgt cccttctccg ctgcgatgtg gatgctcatg
     3601 caatccctcc tagttgttgt cccggcggcg tggtttccgc cccggcccct tcgggcctcg
     3661 gcttccggga ttcgcgccgc tcgcgtatcc gggcaggata cgacggaggc ggtcccaaag
     3721 gctagcgggg cggcgggagg ggccttccgc ggccggggct ggcgcggcgg caaggggcgc
     3781 gggaggccgg acgtcggcgg cgggacggtc cgtggccggg ggcgcgactg cgggacgggg
     3841 ctcggcagaa gcggctcccg gcccgcggac ttggtcccgc cgaggggccg gccgcagggt
     3901 cgcgcggata aagaggtggt ccgaggggag acgggagcgc tgctcgcggt tcagggggat
     3961 ctcgccctcg atcacgcccg cgtccaggac ctcgatgtcc tccgtgcaga agatgtagtc
     4021 gagctgggcg aagttggggc ggggaccgaa gtggtagtgg gtgcggcgcg actcgggcgg
     4081 gacgccgacg gcctccagca cgtcccggaa gggcaggtcg aggaagggtt cgtactcgaa
     4141 ctggtgctcg ccccggatga ggatgccgtt gaagtcgccc aggacgacgc agttcgtcgt
     4201 ccgcaccagg gaagcgagga gctcgacctc cttgatccgc cgctccagtc cccgatcctc
     4261 gccgtactgg gacttgaggt ggagggcgaa gacctcgagg tcgccgccct ccggcccgag
     4321 gtccaggcga aggaggttgc gcgagaaggc gccgggcatg gcgagggcgc gcgcgcccgg
     4381 aaaacggccc ttcctgagga gggcgccgac gaagatgccg cggcgcgagt tctcctcgtg
     4441 gaggaagcag tcgtaggctc cgcggaggta gatccggttg aaggcggcca gggtctccat
     4501 gccgcccacc tcgaccaggg ccacgaggtc gaagtcctcc tcaaggatga tccgcgcgat
     4561 ctcggcgatc ttcccgcggt ccttgttggg gttgtagatg gaggcgttca tcgcgaggta
     4621 gtcggcctcg gtcagggcct cgagctcctc gcggccgtac tccgagtcga ggaggaggta
     4681 gaagttctcg gcgttgaagg aggcgatgtg cagggcttgc gtcacgcgcc tactctagcc
     4741 ggcggcgccg gccgggggga aggggtcgcg gagcgccgac cggcggccga cgcggaagcc
     4801 tggtgggtgt ccgtcacggc gcggctgctt cggcctctcg ccgcatcggc acgggcatcc
     4861 ctcggccgca cggcgcctgg cgcggcggcc tcggacctca ccgcggaggc agggcggagc
     4921 gcgggcctca agcccagagc tcgacttcca tgccggactc ctcgccttgg accacaggcg
     4981 acgccccggc ttcctgccgg cccgcagcct ggtaggacga gtcgcgctcc gcgccgtagg
     5041 cggccgccgc
//
LOCUS       DAOOSJ010000194         5047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10365_length_5047_cov_5.706130, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000194 DAOOSJ010000000
VERSION     DAOOSJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_5047_cov_5.706130"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PLG14_09525"
     CDS             <1..1172
                     /locus_tag="PLG14_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49409.1"
                     /translation="SGWAVQSLLPGDEPPPGTDVLLVLGNSGLDDYAAYRVDAYLAGG
                     GSAVFAVRGVDVEARERLSAKPLADDAILESLAAYGVKVGRSLVLDRSSLTVPFQERN
                     PAGGSAYRYVRYPHWIAARPENADGKHPVTARLSGLDLFWPSPLELSPPSGVAAEPLV
                     RTTPKAWLQTKDFAIAPEEEAYFAREEEATRGQYLLAATLSGRLPMAYSGREIPVREG
                     AAPLPPLPAEAKSSRILVVGSADFANDLMTMTDSLFNAAFVAGGAEWLSSGSELAALK
                     SRGGRDPRLSRIEDPEERNLAALFAYAVNLVLVPGGLAVYGLVRARKRKAEARRKGEA
                     AAPGTEAPAAAAAPHSTAVATAAPAGLADTAAASPGPADMAAGPGDGAGQEGGKR"
     gene            1169..2128
                     /locus_tag="PLG14_09530"
     CDS             1169..2128
                     /locus_tag="PLG14_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOX49410.1"
                     /translation="MMGYRRKVIALSSVLALLLVSLLLGELFSPDRRLARAEEGRLLS
                     GEPKDAAILEISGPEGLLLFERKEGAWLLAEEGGSLPASASRVESFLSEAASVSRLEL
                     RATGRASWPEFGLDGGKGYRVSAKDGKGKSLADFTVGSYGPTGKELFVRLGEAEKSYS
                     AQAPSLASYLAAGRSGWLDLRVSGAPIRPEDVQGVAMRADIALDGPGSPARRFEWRAQ
                     RSEGGWKSGAAALDLLALESLLRGLVNLEAEDARAAAPAGVFDRISLRAELSLGTGVT
                     RIYEAGAEAGGGRYYFRRKDSPIAYEVSAYALKNLAKSLSELK"
     gene            2253..2876
                     /locus_tag="PLG14_09535"
     CDS             2253..2876
                     /locus_tag="PLG14_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49411.1"
                     /translation="MAGDLRDRIIAGGAEALRDSGLKGFSMEQAALRAGVSRKTVYNY
                     FSSRFELLDGVLEARARETMEVLGAIASDASLGFVEKLNRVTEEGFRRGREGTRFLRG
                     DGSYPKSAELYRALRANLAEFITGIVSEAAGLGLIREDVDPRRLTYVILNMVSGLLDL
                     AEPEDEPYTRIELLQESLRLLVGGILSPEGAETLKGYSLLARPEARP"
     gene            2873..3946
                     /locus_tag="PLG14_09540"
     CDS             2873..3946
                     /locus_tag="PLG14_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX49412.1"
                     /translation="MKTKTTARRAAAWLALLALLGGAAALRLLSAGKEGKAEAAMVAP
                     VRVVQASFGPLVKELKVNAFVESDSMVTVLPLVSGILQTLEVEAGDRVRKGQVLARID
                     AARYELQLGQAEAAWLSAKSTFERVEQLFRAGATSSQNYDQAKGQYDAYRAQYDLAKL
                     QAGYAAVTSPVDGVVLVRHTTVGSIAAPERPLVTIGDLSRLVIRARVPERYYAEFRGT
                     GAPVAVRVERSDGGSFPARLSSVSPYISAENRTFEATCDLGPGQDVLRPGMSVVAVFV
                     IERRDGVWSLPFEVLVGGDTAWYVEEDGAGQAVARKLALPPGFSSEARFEIPEGEAGK
                     TFIVEGQHFIRDGAPVRVAAGAP"
     gene            3943..>5047
                     /locus_tag="PLG14_09545"
     CDS             3943..>5047
                     /locus_tag="PLG14_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX49413.1"
                     /translation="MKASDFSIRHPAIVGILLASLLVFALLSYSSLNREMIPSVGLPA
                     ANVITAYPGAGAEEIERTVTREIEDQLSTLAGVSSMKSTSKDSYSVVSVDFRDGVDVY
                     EKLPQIRELLNGVRDRLPEGLRGEPEIIVSEASGILPIFSVRVDSDIDRVVLAKRVEE
                     DLVPRLTRIKGVSKVNVRGGAKRRVRVDLDLDRLEARGISPLQAYEALRYANRNLPAG
                     SADWRGSRLSLTTMGALASLEEAEGVAVGQKDGSAIYLGDVAQVAFELEPPEYRILSG
                     GRDVLVVDILKREDGNTIDIVRDARRELDAFSARQGGAAGWKVLTDQAETTEASLAVV
                     VQSAFMGTLLAVLVILLFLHDWRATLIIGASIPL"
BASE COUNT          711 a         1906 c         1707 g          723 t
ORIGIN      
        1 cctcgggctg ggccgtccag tccctcctcc ccggcgacga gccgcccccc gggaccgacg
       61 tcctcctcgt cctggggaac tcagggctgg acgactatgc cgcctatcgc gtcgacgcct
      121 acctggcggg gggcggctcc gccgtcttcg ccgtccgcgg cgtcgacgtg gaggcgaggg
      181 agcgcctctc cgcgaagccc ctcgcggacg acgcgatcct ggagagcctc gcggcctacg
      241 gcgtcaaggt ggggcgctcg ctcgtcctgg accgctcctc gctgaccgtg cccttccagg
      301 agcggaatcc cgcgggcggc tcggcctacc gctacgtccg ctacccgcac tggatagccg
      361 cgcggccgga gaacgccgac gggaagcacc ccgtcaccgc ccgcctctcc ggcctggacc
      421 tcttctggcc gagccccctc gagctctccc cgccttcggg cgtcgcggcc gagcccctcg
      481 tcaggaccac gcccaaggcc tggctccaga ccaaggactt cgccatcgcc cccgaggagg
      541 aagcctactt cgccagggaa gaggaggcga cgaggggaca gtacctcctg gcggcgaccc
      601 tctccgggcg cctccccatg gcctactcag gcagggaaat ccccgtccgc gagggcgccg
      661 cccctctgcc gcccctgccc gcggaggcca agtcctccag gatcctcgtc gtgggctcgg
      721 ccgacttcgc gaacgacctc atgacgatga ccgattccct cttcaacgcg gccttcgtag
      781 ccggaggagc cgagtggctc tcctccggct ccgagctcgc cgccctcaag tcccgcggag
      841 gccgcgaccc gcggctctcc cgcatcgagg atcccgagga gcgcaacctc gccgccctct
      901 tcgcctacgc cgtgaacctc gtcctcgtgc cgggaggcct cgcggtctac ggactcgttc
      961 gggcaaggaa aaggaaggcc gaggcgagga gaaagggcga ggcagccgcg ccgggcacgg
     1021 aggcgccggc cgcggccgcc gcgcctcact cgaccgccgt cgcgactgcc gcgcctgccg
     1081 ggctcgccga cacggccgcg gcgtcgcccg ggcccgccga catggccgca gggcctggcg
     1141 acggagccgg ccaggaagga ggcaaacgat gatgggctac cggcggaagg tgatcgctct
     1201 ctcctcggtc ctcgcccttc tcctcgtgtc gctcctcctc ggggagctct tttcccccga
     1261 ccggcggctc gcccgcgcgg aggaaggccg gctgctctcg ggcgagccta aggacgcggc
     1321 catcctcgag atctccggcc ccgagggcct cctcctcttc gagaggaagg agggagcctg
     1381 gctcctcgcc gaggagggcg ggagcctccc ggcctcggcc tcgcgcgtgg agagcttcct
     1441 ctccgaggcc gcctccgtct ccaggctgga gctccgggcg acgggcaggg cttcctggcc
     1501 cgagttcggc ctggacgggg ggaagggcta ccgcgtgtcc gcgaaggacg gcaagggcaa
     1561 gtccctcgcc gatttcacgg tcggctccta cggcccgacg gggaaggaac ttttcgtgcg
     1621 cctgggggag gcggagaaga gctactcggc ccaggccccg agcctggcct cctacctcgc
     1681 cgcgggaagg agcggctggc tcgacctccg cgtctcgggc gcgcccatcc ggcccgagga
     1741 cgtgcaggga gtcgcgatgc gcgccgacat cgcgctggac ggccccggct ccccggcccg
     1801 ccgcttcgag tggagggcac agaggagcga gggtggctgg aagtccggcg ccgcggccct
     1861 cgacctcctc gccctcgagt ccctcctccg cggcctcgtg aacctggagg cggaggacgc
     1921 ccgcgccgcc gcccccgccg gcgtcttcga ccgcatcagc ctgcgcgccg agctctcgct
     1981 cggtacggga gtcacgagga tctacgaggc gggggccgag gcgggcgggg ggcgctacta
     2041 tttccggcgc aaagactccc ccatcgccta cgaggtctcg gcctacgccc tcaagaatct
     2101 tgccaaatca ttgtctgaac tgaaataaca accagcgcgg tcccggggct tcggtcctgg
     2161 gggcgggcgc ttgaagggcg gggcagggct tgaccgaagg ccttgcctcg cctattcttt
     2221 acacatggtg agtaaaatgt gtaaggcgag ccatggccgg tgaccttcgg gaccgcatca
     2281 tcgcgggcgg agccgaggcc ctcagggact cgggcctcaa gggcttcagc atggagcagg
     2341 ccgcgctcag ggcgggcgtc agccgaaaga ctgtctacaa ctatttctcc tcgcgcttcg
     2401 agctcctgga cggcgtcctc gaggcccgcg ccagggagac gatggaggtc ctgggggcga
     2461 tcgcctccga cgcctccctc ggcttcgtgg agaagctcaa ccgcgtcacg gaggagggct
     2521 tcaggcgcgg ccgcgaggga acgcgcttcc tcagggggga cggctcctac ccgaagtccg
     2581 ccgagctcta ccgggccctc cgcgcgaacc tcgccgaatt catcaccggc atcgtctccg
     2641 aggccgccgg cctcggcctc atccgcgagg acgtcgatcc ccgccgcctc acctacgtca
     2701 tcctcaacat ggtctcgggc ctcctcgacc tcgcggagcc cgaggacgag ccctatacgc
     2761 ggatcgagct cctgcaggag tccctcaggc tcctcgtggg gggcatcctc tcccccgaag
     2821 gcgcggagac cctcaagggc tacagcctcc tcgcgaggcc ggaggccagg ccgtgaagac
     2881 gaagaccacc gcgcggcgcg ccgccgcctg gctcgccctc ctcgccctcc tgggcggggc
     2941 cgcggccctg aggctcctgt cggccgggaa ggagggcaag gccgaggccg cgatggtcgc
     3001 ccccgtccgg gtcgtccagg cctccttcgg gcccctcgtc aaggagctca aggtcaacgc
     3061 cttcgtggag agcgacagca tggtcaccgt cctgcccctc gtctccggca tcctccagac
     3121 cctggaggtg gaggcgggcg accgggtcag gaaagggcag gtcctggcca ggatcgacgc
     3181 ggcgcgctac gagctccagc tcggccaggc cgaggcggcc tggctctccg cgaagagcac
     3241 cttcgagcgg gtggagcagc tcttccgggc cggggcgacg agcagccaga actacgacca
     3301 ggccaagggg cagtacgacg cctaccgcgc ccagtacgac ctggcgaagc tccaggcggg
     3361 ctacgccgcc gtcacgagcc ccgtggacgg cgtcgtcctc gtgcgccaca cgacggtcgg
     3421 ctccatcgcc gcgcccgagc gtcccctcgt cacgataggc gacctctccc gcctcgtcat
     3481 ccgggcccgc gtgcccgagc gctactacgc ggaattccgc gggaccggcg ctcccgtggc
     3541 ggtccgggtg gagcggagcg acggcggctc cttcccggcc aggctctcct cggtctcccc
     3601 ctacatctcg gcggagaacc gcaccttcga ggcgacctgc gacctcggtc ccggccagga
     3661 cgtcctgcgg cccggcatgt ccgtggtcgc ggtcttcgtg atcgagcgcc gcgacggcgt
     3721 ctggtccctt cccttcgagg tcctggtggg cggggacacg gcctggtacg tggaggagga
     3781 cggcgctggc caggcggtgg cccggaagct cgccctcccg ccgggcttct cgagcgaggc
     3841 ccgcttcgag atccccgagg gcgaggccgg caagaccttc atcgtcgaag ggcagcactt
     3901 catccgcgac ggggcgcccg tccgcgtcgc ggccggggcc ccatgaaggc ctccgacttc
     3961 tcgatccggc acccggcgat cgtcggcatc ctcctcgcct ccctcctcgt cttcgccctc
     4021 ctatcctact cctcgctcaa ccgcgagatg atcccctccg tcggcctccc cgcggcgaac
     4081 gtgatcaccg cctacccggg cgcgggcgcc gaggagatcg agcggaccgt gacgagggag
     4141 atcgaggacc agctgtccac gctggccggc gtctcctcca tgaagtcgac gagcaaggac
     4201 tcctactccg tcgtctccgt cgatttccgg gacggcgtcg acgtctacga gaagctcccg
     4261 cagatccgcg agctcctgaa cggcgtccgg gaccgcctgc ccgagggcct gcgcggcgag
     4321 ccggagatca tcgtgagcga ggcgagcggc atcctcccga tcttctccgt ccgcgtcgac
     4381 tccgacatcg accgcgtcgt cctcgcgaag cgggtggagg aggacctcgt gccccgcctc
     4441 acccgcatca agggcgtctc caaggtcaac gtccgcggcg gagccaagcg gcgcgtcagg
     4501 gtcgacctgg acctggaccg cctggaggcc aggggcatct cgcccctcca ggcctacgag
     4561 gccctccgct acgcgaaccg caacctcccc gcgggcagcg cggactggcg gggctcgcgc
     4621 ctctccctca cgacgatggg cgccctcgcg agcctggagg aggcggaggg cgtggccgta
     4681 ggccagaagg acggctcggc catctacctg ggcgacgtcg cccaggtggc cttcgagctc
     4741 gagcccccgg agtaccggat cctctccggg ggccgcgacg tcctggtcgt cgacatcctg
     4801 aagcgcgagg acggcaacac gatcgacatc gtgcgcgacg cgcggcgcga gctcgacgcc
     4861 ttctccgcgc ggcagggcgg agcggccggc tggaaggtcc tcaccgacca ggcggagacg
     4921 acggaggcct ccctcgccgt cgtggtccag agcgccttca tgggcaccct cctcgccgtc
     4981 ctcgtcatcc ttctcttcct ccacgactgg agggcgaccc tcatcatcgg cgcgagcatc
     5041 cccctct
//
LOCUS       DAOOSJ010000195         5036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10403_length_5036_cov_5.755872, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000195 DAOOSJ010000000
VERSION     DAOOSJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10403_length_5036_cov_5.755872"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLG14_09550"
     CDS             complement(<1..305)
                     /locus_tag="PLG14_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49414.1"
                     /translation="MKRSIALVLMLVAFSVLVFGQVKAKDGVYFAKESAFSKSGWMDE
                     VVVTVAGGKITKVLWNGVPNLPGATDKKSFAASGKYGMAKAAKAGEWDKQAAAAEAWL
                     "
     gene            complement(398..703)
                     /locus_tag="PLG14_09555"
     CDS             complement(398..703)
                     /locus_tag="PLG14_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOX49415.1"
                     /translation="MLKKDYAKNRKTCKVTFSLPARVAADAKTVTLVGDFNSWSQEAT
                     PLKLGEDGLWTATLALEAGREYHFRYLIDGRTWENDWNADKYVPSPYGDADNSVVIV"
     gene            911..2206
                     /locus_tag="PLG14_09560"
     CDS             911..2206
                     /locus_tag="PLG14_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX49416.1"
                     /translation="MNALAEELNKALAGTVAERLLADMGRRMYFPKGILSQSAEADAR
                     ANRMNATIGMAYEGGAPLILDAIRSALPTLSPAEAVAYAPTSGVAALRKRWKAELEKK
                     NPSLKGKSYSLPVVVPGLTAAISYIADLFLEPGDTVVVPDLHWPNYRLIIEERKMAAG
                     LTYPIFKDGGFNVAGLEAGLREAGGRRGKAACILNFPNNPTGYSPTLAEADAIAAALR
                     RVAEEGTDLLVIADDAYFGLQYEPGLLEESLFSRLADAHERILAVKADGPTKEDYVWG
                     FRTGFVSFAGKELGEAQHEALVKKLMGVIRSSVSNSSAPVQHLLLKALDSPGYAEQKA
                     RYRGLLEARYAKVRAFLARASLPASLRLLPFNSGYFMSFECVGVSAEALRLRLLDKGI
                     GVISMQDRYLRVAFSSVEAASIEALYAAIFEEAAALAKA"
     gene            2226..>2584
                     /locus_tag="PLG14_09565"
     CDS             2226..>2584
                     /locus_tag="PLG14_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOX49417.1"
                     /translation="MGVSRASLSSAAILLAAFALGLPGAAPAGAAPSPSPAKAPAAAA
                     SAPATPGQPDAEGRFEGLASWYGAQFHGRQTSNGEIYDKEGLTAAHRSLPFGTLLRVT
                     SFDTGASVVVRVNDRGP"
     assembly_gap    2585..2684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3190..4461
                     /locus_tag="PLG14_09570"
     CDS             3190..4461
                     /locus_tag="PLG14_09570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOX49418.1"
                     /translation="MKKIALAYSGGLDTTVIVPWLKENYDCEVVAVCGDVGQDCDWAS
                     LEKRAVASGAAKLVVRDLKAEFVEGYLWPLVKSGAAYEGRYLLGTSAARPLLAKCLAE
                     TAVKEGCSAVAHGCTGKGNDQVRFELGVKAFAPEMEVIAPWRIWDIQSREEEIEYLEA
                     RGIPVPMKKSDSYSRDDNLWHVSHEGLDLEDPANEPKLEGMLKMSTPPEKAPDVPEYV
                     ELGFGQGVPVSLNGKAMDGLSLVKALNAIAGRHGVGILDMVENRVVGMKSRGVYETPG
                     GAVIMEAHARLEQLCLDRKTLSFKLGVAQRFAETLYEGEWFCPLREALSAFIDSTQGR
                     VTGTVRLKLWKGSISGAGAKSPYSLYDASLASFTTGPLFSHKDAEGFINLFGLPTKVR
                     ARLEARLTSGGGAAAAAIVPGSAGYTSPAGD"
     gene            4490..>5036
                     /locus_tag="PLG14_09575"
     CDS             4490..>5036
                     /locus_tag="PLG14_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOX49419.1"
                     /translation="MAKLWETREGGDYRLDPGVEAFLSSVSVDSRLLEEDLAGSLAHA
                     AMLGEAGIISPAAAEALRGGLEALAAEAEAGSLAVDPGAEDVHSFVEAELTARLGDAG
                     RSLHAGRSRNDQVALDLRLWLRSASVALRASLLDAIEAAAALAAGNLGTIMPGYTHLQ
                     RAQPVTFAYHLLAWCAALERDY"
BASE COUNT          793 a         1777 c         1605 g          761 t
ORIGIN      
        1 agccaggcct cggcggccgc ggcctgcttg tcccactcgc cggccttggc ggccttggcc
       61 atgccgtact tgccggaggc ggcgaagctc ttcttgtcgg tcgcgccggg gaggttgggg
      121 acgccgttcc agaggacctt ggtgatcttg ccgccggcga cggtgacgac gacctcgtcc
      181 atccagccgg acttggagaa ggcgctttcc ttggcgaaat agacgccgtc cttggccttg
      241 acctggccga agacgaggac tgagaaggcg acgagcatga gaacgagagc gatactgcgc
      301 ttcatcgata accccctggg aatattggag aatcaagtaa actagatata aagagcactt
      361 cttgcggcaa tgcgtcaagc actggggcgt ggggttatca aacgataacc accgagttgt
      421 cggcgtcgcc gtagggcgag gggacgtact tgtcggcgtt ccagtcgttt tcccaggttc
      481 gtccgtcgat gaggtagcgg aagtggtact cgcgtcccgc ctcgagggcc agggtggccg
      541 tccagagtcc gtcctctcca agcttgagcg gagtcgcctc ctggctccag ctgttgaagt
      601 cgccgacgag ggtcacggtc ttcgcgtcgg cggcgacgcg ggccggcagg ctgaaggtca
      661 ccttgcaggt cttgcggttc ttcgcgtagt cctttttgag catacttccc tccgcacccg
      721 cgaattctac cgcatcggcg cgccggccgc cagggctcgg ggggcggcgc aggccgcggc
      781 gaggcggccg cggcggtcct cgggcccttc cgccggcggg tcccgcgcgg cccttggtcc
      841 gggccgccga attgacagct tcggctttcg cgtttaccct ggtcgaatca cctcttaggg
      901 agaatcctcg atgaacgctc tcgccgaaga actcaacaag gcgctcgccg gcaccgtcgc
      961 cgagcgcctc ctcgccgaca tgggccggag aatgtacttt cccaagggca tcctctccca
     1021 gagcgccgag gccgacgcgc gggccaatcg catgaacgcc acgatcggga tggcctacga
     1081 gggaggcgcc cccctcatcc tcgacgccat ccgctccgcc ctgcccaccc tgagccccgc
     1141 ggaggccgtc gcctacgccc cgacctccgg cgtcgccgcg ctcaggaagc gctggaaggc
     1201 cgagctcgag aagaagaacc cgagcctcaa gggcaagtcc tattccctcc ccgtcgtcgt
     1261 ccccggcctc acggccgcga tctcctacat cgccgacctc ttcctggagc cgggcgacac
     1321 cgtcgtcgtc cccgacctcc actggccgaa ctaccgcctc atcatcgagg agaggaagat
     1381 ggccgccggc ctcacctatc ccatcttcaa ggacggcggc ttcaacgtgg ccggcctcga
     1441 ggccgggctc cgcgaggcgg ggggaaggag gggcaaggcg gcctgcatcc tcaacttccc
     1501 gaacaacccc acgggctact cgccgaccct cgcggaggcc gacgccatcg ccgcagccct
     1561 gcgccgcgtc gccgaggagg gcaccgacct actcgtcatc gccgacgacg cctatttcgg
     1621 cctccagtac gagccgggcc tcctcgagga gtccctcttc agccgcctcg ccgacgccca
     1681 cgagcgcatc ctagccgtga aggccgacgg gcccacgaag gaggactacg tctggggctt
     1741 ccgcaccggc ttcgtgagct tcgcgggcaa ggagctcggc gaggcccagc acgaggccct
     1801 cgtgaagaag ctcatgggcg tgatccgctc ctcggtctcc aactcctccg ccccggtcca
     1861 gcacctcctc ctcaaggccc tcgactcgcc cggctacgcc gagcagaagg cccgctaccg
     1921 cggcctcctc gaggcgcgct acgcgaaggt ccgcgccttc ctcgcccgcg cgagcctccc
     1981 cgcgagcctc aggctcctgc ccttcaactc gggctacttc atgagcttcg agtgcgtcgg
     2041 cgtctcggcc gaggccctgc ggctccgcct cctcgacaag gggatcggcg tcatctcgat
     2101 gcaggaccgc tacctccgcg tggccttctc cagcgtcgag gccgcctcca tcgaagcgct
     2161 ctacgctgcc atcttcgaag aggcggccgc cctcgcgaag gcctagggcc tcggggggct
     2221 cgcgcatggg cgtctctcgg gcaagcctct cgtcggccgc catcctcctc gcggccttcg
     2281 ccctggggct gcccggggcg gcgcccgccg gggccgcccc gtccccgtcc ccggccaagg
     2341 cgccggcggc cgcggcttcg gccccggcga cgcctggtca gcccgacgcc gagggccgct
     2401 tcgagggcct cgcgagctgg tacggggccc agttccacgg caggcagacc tccaacggcg
     2461 agatctacga caaggagggc ctcaccgcgg cccaccggag cctgcccttc ggaaccctcc
     2521 tccgcgtgac gagcttcgac accggggcga gcgtcgtcgt ccgcgtgaac gaccgcggcc
     2581 ccttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggatcg tcttccccct
     2701 cgtgcccgcc gctgaggccg agaccctcgc cgccaggcta cgcgcgatgg gctaccgcga
     2761 cctcctcgtg accaggacac tcgcctcgag gtagcgaaag cgaccttatc cctactcatc
     2821 gattatctct cagggtgaat atatctttct ctgcaaaatt tgcaccgcgg aattattatc
     2881 catgaacgct tcagtgatgc aataattgcg tcctagaaat gcataaaaat acataaaagt
     2941 cacaagaaag cgtataataa tacttgacca cgcggagaag ccaggatatg gtaaagccct
     3001 catggctacc caaggtttcg gcgcttgcct cgaaccgcgc ggagcgaccc gctccgagct
     3061 ttccccccgc cccgcccgcc tccgtcgccg ctgacactcc cccctacgac acaaccagtc
     3121 catccaacgc ggccccggca taggggaagc gcatacgcgc gccttcgcgg cgcgaagaag
     3181 gagcgaacca tgaagaagat agccctggcc tattcgggcg gcctcgacac caccgtcatc
     3241 gtgccctggc tcaaggagaa ctacgactgc gaggtcgtcg ccgtctgcgg cgacgtcgga
     3301 caggactgcg actgggcctc cctggagaag cgcgccgtcg cctcgggcgc cgcgaagctc
     3361 gtcgtgcggg acctcaaggc ggagttcgtc gagggctacc tatggcccct cgtgaagtcg
     3421 ggcgcggcct acgagggccg ctacctcctc gggacctccg cggcccggcc actcctcgcc
     3481 aagtgccttg cggagacggc cgtcaaggaa ggctgctccg cggttgccca cggctgcacg
     3541 ggcaagggca acgaccaggt ccgcttcgag ctcggcgtca aggccttcgc tcccgagatg
     3601 gaggtgatcg cgccctggcg gatctgggac atccagagcc gggaggagga gatagagtac
     3661 ctcgaggcgc ggggcatccc ggtgccgatg aagaagtccg actcctacag ccgggacgac
     3721 aacctctggc acgtgagcca cgagggcctg gacctcgagg atcccgcgaa cgagcccaag
     3781 ctcgagggga tgctcaagat gtcgaccccg cccgaaaagg cgcccgacgt ccccgagtac
     3841 gtggagctcg gcttcgggca gggcgtgccg gtgagcctga acggcaaggc gatggacggc
     3901 ctctccctcg tgaaggcgct caacgcgata gcgggccggc acggcgtcgg catcctcgac
     3961 atggtggaga accgcgtggt cggaatgaag tcgaggggcg tctacgagac cccgggcggc
     4021 gcggtgatca tggaggcgca cgccaggctc gagcagctct gcctggaccg gaagacgctc
     4081 tccttcaagc tcggcgtcgc gcaacgcttc gcggagaccc tctacgaggg cgagtggttc
     4141 tgccccctca gggaggccct ctccgccttc atcgactcga cgcaagggag ggtcacgggg
     4201 acggtcaggc tcaagctctg gaagggctcg atctccgggg ctggcgccaa gtcgccctat
     4261 tccctctacg acgcgagcct ggcgagcttc acgacggggc ccctcttctc gcacaaggac
     4321 gccgagggct tcatcaacct cttcggcctc ccgaccaagg tccgggcgag gctggaggcg
     4381 aggctcacgt cggggggcgg cgccgcggcc gccgcgatag tccccggctc cgcgggctac
     4441 acttcgcccg cgggcgactg acggaaggcg ggagagggga gggggagcca tggccaagtt
     4501 gtgggaaacc agggagggcg gggactaccg cctcgatccg ggcgtcgagg ccttcctgtc
     4561 ctcggtctcc gtcgactcgc ggctcctcga ggaggacctc gcaggctccc tcgcccacgc
     4621 cgcgatgctc ggcgaggccg gcatcataag ccccgcggcg gcggaggccc tccgcggcgg
     4681 cctcgaggcc ctcgcggccg aggccgaggc ggggagcctc gcggtggacc cgggggccga
     4741 ggacgtgcac tccttcgtcg aggcggagct caccgcccgc ctcggggacg cggggaggag
     4801 cctccacgcg gggcggagcc ggaacgacca ggtcgccctg gacctcaggc tctggctccg
     4861 ctcggcctcg gttgcgctcc gcgcctccct cctcgacgcg atcgaggccg ccgcggccct
     4921 cgccgcgggg aacctcggga ccatcatgcc gggctacacc catctccagc gcgcccagcc
     4981 cgtcaccttc gcctaccacc tcctggcctg gtgcgcggcc ctggagcggg actacg
//
LOCUS       DAOOSJ010000196         5035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10407_length_5035_cov_6.515261, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000196 DAOOSJ010000000
VERSION     DAOOSJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_5035_cov_6.515261"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..2058)
                     /gene="aspS"
                     /locus_tag="PLG14_09580"
     CDS             complement(187..2058)
                     /gene="aspS"
                     /locus_tag="PLG14_09580"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOX49420.1"
                     /translation="MEYLKRTVTCGELRAQDAGKTVVLNGWVHRKREHGPITFVNLRD
                     RYGITQVVVDADAEAALKAAAAELKYEYCIAVKGAVRARPDTMVNPDMATGAVEVVAL
                     ELQILTRCETLPFMVDEKSDAKEDLRLKYRYLDLRSFSMQDKIRLRHEVAFAVREHLV
                     SKGFYEIETPTFIRSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQILMVSGMDKYFQ
                     LARCYRDEDARGDRQPEFTQVDIEMSFVKRDDVLELVEGMMGHVFRKTLGVELPAKFR
                     RIAYDDSIELYGTDKPDLRFGMEMKDFGELAAKSGFQAFKDALAEGAARQAAAKAKGI
                     TQEGGAVKALLVPGKADYSRKQIEELEAAAKIYRAKGMAWMKVNAEGRLEGGASKFFA
                     GEAAPAGLEAEVVAYLGAKPGDLILLVADAKRRVACVSLGAVRSKLGKDLGFCDPKRF
                     EFAWIVDFPMFEYNEEEAKWDTAHHMFTMPQEKYHPTLESDPGAVKGDLYDLVMNGFE
                     LASGSIRIHDPELQKRIFSIVGFDPAEAERKFGFLTEAFKYGPPPHGGIAPGLDRLVM
                     LMAGETSIKEVIAFPKNSFAVSPMDESPNEVDAKQLEELSIRIVEGADGAKTVEIQK"
     gene            complement(2465..3667)
                     /locus_tag="PLG14_09585"
     CDS             complement(2465..3667)
                     /locus_tag="PLG14_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOX49421.1"
                     /translation="MHENEARRARELTRDLIERHGPRLAGSPACLAAADELAGLAAAR
                     ADRVLKEDFAVRPGAFLGFIKVMVALYVLSCLALPFAPWASVAGLGLGAVVLVLEFFL
                     YKELIDPFYPLKTGRNVTAVLEPLGEAKRQVVVSGHHDSARVFLFYVNRPELYAARLY
                     GGMGAYFAFLAAALGLAALGAPRPLLVGASALFAALLVLVAPLWRFALAEGTPGAGDN
                     LAASAAALEILGHFKERRDAGKGLESTRLVFASFDAEEAGLRGARAWAKARSAELAST
                     PTFVYNMDCMYEAKDARFLSSDLNGSVALDARTAAACAEAARRRGVAAPVEPIAFLTG
                     GTDAAELAKKGARATTFIGMRWSNEARGSGYHTPGDTVEAVSPEVLELAIGVGVDFIE
                     RLDSGELD"
     gene            3845..>5035
                     /locus_tag="PLG14_09590"
     CDS             3845..>5035
                     /locus_tag="PLG14_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOX49422.1"
                     /translation="MPELDPIAYIPASSGAASEAKAAAKVRAKPAAPRLLSLDASMAN
                     RHGIVAGATGTGKTVTLHSLAERLSNAGVAVFLADAKGDLSGLAAPGEAFSEGPPKVG
                     NAKIDAAIEASGLSGRPRPGLPAVFWDVFGEKGHPLRATVSEMGPLLLGRALGLSETQ
                     AEVLSVVFRVADDRGLLLLDLKDLRAVLEFCSSNAQALEPDYGRMAPASLGAIQRGIV
                     VLQGQGGDLFFGEPALDIGELLRKDEKGRGLVHVLAADRLLEAPSLYAAFMLWLLSEL
                     FEELPEEGDLPAPKLVFFFDEAHLLFDGAPEELVRSVARTVRLVRSKGVGVWFVTQNP
                     TDLPEEVLAQLGNRIQHALRAYTEKERRSLKAAADSFRPNPKLDAEATLGELGVGEAL
                     VSLLD"
BASE COUNT          692 a         1790 c         1768 g          785 t
ORIGIN      
        1 ctcttcggcg acgatggcga gggcgcggtt ggtgttgccc tccgcgcggg gacttccgtt
       61 gaacgcgacg accttcatgc tgcctcctct gaatagggaa acgagggggc cgccctcgcg
      121 gacggccccc cggagcccgc gccgcccgcc gccccgcggg gcggcgggcc ggccggtgcg
      181 cccgccttac ttctggatct cgaccgtctt cgcgccgtcc gcgccctcga cgatgcggat
      241 ggagagctcc tcgagctgct tcgcgtcgac ctcgttcggc gactcgtcca tggggctcac
      301 cgcgaaggag ttcttcggga aggcgatgac ctccttgatg gaggtctcgc cggccatcag
      361 catcacgagg cggtccaggc cgggcgcgat gccgccgtgg gggggcgggc cgtacttgaa
      421 ggcctccgtg aggaagccga acttccgctc cgcctccgcg ggatcgaagc cgacgatgga
      481 gaagatgcgc ttctggagct cggggtcgtg gatgcggatg gagcccgagg cgagctcgaa
      541 gccgttcatc acgaggtcgt agaggtcgcc cttcacggcg cccgggtccg actcgagggt
      601 cgggtggtac ttctcctggg gcatcgtgaa catgtggtgg gcggtgtccc acttcgcctc
      661 ttcctcgttg tactcgaaca tcgggaagtc gacgatccag gcgaactcga agcgcttcgg
      721 gtcgcagaag cccaggtcct tgccgagctt ggagcgcacg gcgcccaggg agacgcaggc
      781 gacgcggcgc ttcgcgtccg cgacgaggag gatgaggtcg ccgggcttgg cgccgaggta
      841 ggcgacgacc tccgcctcga ggcccgcggg ggcggcctct ccggcgaaga acttggaggc
      901 cccgccctcg agcctgccct ccgcgttgac cttcatccag gccatgccct tggcgcggta
      961 gatcttcgcc gccgcctcga gctcctcgat ctgcttgcgg gagtagtcgg ccttgcccgg
     1021 gacgaggagg gctttcacgg cgccgccctc ctgggtgatg cccttggcct tggcggcggc
     1081 ctggcgggcc gcgccttcgg ccagggcgtc cttgaaggcc tggaagccgg acttcgcggc
     1141 gagctcgccg aagtccttca tctccatgcc gaagcggagg tccggcttgt cggtgccgta
     1201 gagctcgatc gagtcgtcgt aggcgatgcg gcggaacttg gccggcagct cgacgccgag
     1261 ggtcttcctg aagacgtggc ccatcatgcc ctcgacgagc tccaggacgt cgtcgcgctt
     1321 cacgaaggac atctcgatgt cgacctgcgt gaactccggc tggcggtcgc cgcgggcgtc
     1381 ctcgtcgcgg tagcagcggg cgagctggaa gtacttgtcc atgcccgaca ccatgaggat
     1441 ctgcttgtag agctggggcg actggggaag ggcgtagaac ttgcccgggt agaggcgcga
     1501 ggggacgagg tagtcgcgcg cgccctcggg cgtcgagcgg atgaaggtcg gcgtctcgat
     1561 ctcgtagaag cccttcgaca cgaggtgctc gcggaccgcg aaggcgacct cgtggcggag
     1621 gcggatcttg tcctgcatcg agaaggaccg caagtcgagg tagcggtact tgaggcggag
     1681 gtcctccttc gcgtcggact tctcgtccac catgaagggg agggtctcgc agcgggtgag
     1741 gatctggagc tccagggcca cgacctcgac cgcgccggtc gccatgtcgg ggttgaccat
     1801 ggtgtcggga cgggcgcgga cggcgccctt gaccgcgatg cagtactcgt acttgagctc
     1861 ggcggcggcg gccttcaagg ccgcctcggc gtcggcgtcc acgaccacct gcgtgattcc
     1921 gtagcggtcg cggagattca cgaaggtgat ggggccgtgc tcgcgcttgc gatggaccca
     1981 gccgttgagg acgacggtct ttccggcgtc ttgggcgcgg agctctccgc aggtgacggt
     2041 gcgcttcagg tattccatgt gacccctcca agatagacaa gccccccgtc tttgcggacg
     2101 aggggctctc tcgtggtgcc accgcaattc gcccaaggcg ccggagtggg agcgcctcga
     2161 gcctttaagg gagcccgtga cgggggctcg gtccggcggg acttactggg cggccttttc
     2221 cgggtccgct ttttttcccc gcgctcggga gggtctttcg ggaggctgcc tcgtccgcct
     2281 tctcagctgc cggcggctct ctgtgcgagg gcgcgttcct tcctactctt ctccgtcaac
     2341 gctttagcgg caatcataca ggggcgggcg gcccctgtca acggaagcga tcgggggcgg
     2401 cgcgccgccc ggcggcggcc ctgcaagccc gccggccgcc cgtccgccgg ccgccttcct
     2461 cggcctagtc gagctcgccc gagtctaggc gctcgatgaa atcgacgccg accccgatcg
     2521 cgagctccag tacctcgggg ctgaccgcct cgaccgtgtc gcccggcgtg tggtagcccg
     2581 agcctcgcgc ctcgttcgac cagcgcatcc cgatgaaggt cgtggccctg gcgcccttct
     2641 tcgcgagctc ggcggcgtcc gtgccgcccg tcaggaaggc gatgggctcc accggcgccg
     2701 cgacgccgcg cctgcgggcc gcctccgcgc aggccgcggc cgtccgcgcg tcgagcgcca
     2761 ccgagccgtt gaggtcgctc gagaggaagc gcgcgtcctt ggcctcgtac atgcagtcca
     2821 tgttgtagac gaaggtgggc gtcgaggcga gctccgccga gcgagccttc gcccaggccc
     2881 gcgcgccgcg gagacccgcc tcctcggcgt cgaagctcgc gaagacgagc cgcgtcgact
     2941 cgaggccctt ccccgcgtcg cggcgctcct tgaagtggcc caggatctcg agggcggcgg
     3001 cgctcgccgc gaggttgtcg cccgcgcctg gcgtgccctc cgcgagggcg aagcgccaca
     3061 gcggcgccac gaggacgagg agggccgcga agagggccga ggccccgacg aggaggggcc
     3121 tcggcgcgcc cagcgccgcg aggccgaggg ccgccgcgag gaaggcgaag taggcgccca
     3181 tgcctccgta gagccgagcg gcgtagagct cgggcctgtt cacgtagaag aggaagacgc
     3241 gggccgagtc gtggtggccc gagacgacca cctggcgctt cgcctcgccc agcggctcga
     3301 ggacggccgt gacgttccgc cccgtcttta gcgggtagaa gggatcgatg agctctttgt
     3361 agaggaagaa ctcgaggacg aggaccacgg ccccgaggcc gagccccgca accgaggccc
     3421 agggcgcgaa ggggagggcg aggcaggaga ggacgtagag ggcgaccatc accttgatga
     3481 agccgaggaa ggcccccggc cggaccgcga agtcctcctt aaggacccgg tcggcgcgcg
     3541 ccgccgcgag gccggcgagc tcgtccgccg cggccaggca ggcggggctt cccgcgagcc
     3601 gcggcccgtg ccgctcgatg aggtctctcg tgagctcgcg cgcgcggcgc gcctcgttct
     3661 cgtgcatgtt ccgctcctcc cccggaccgg gccgggcctc cggaggatag catggaaagg
     3721 cccgcgcgcg gctcaccttt ggcccgaggc gccagtttag catatgctaa accgagcccg
     3781 cccttgcggg cctcgccagc ccggcctacc atggaaagcg aacccgcctt tcgaggagcg
     3841 ccccatgccc gagctcgatc caatcgccta cattcccgcc tctagcggcg ccgcgtccga
     3901 ggcaaaagcc gccgcgaagg tccgcgccaa gcccgccgcg ccccgcctcc tctccctcga
     3961 cgcctcgatg gcgaaccgcc acggcatcgt cgccggagcg acgggcacgg ggaagaccgt
     4021 caccctccat tccctggccg agcggctctc caacgccggc gtcgcggtct tcctggccga
     4081 cgcgaagggc gacctctcgg gcctcgcggc gcctggcgag gccttctccg aggggccgcc
     4141 caaggtcggc aacgcgaaga tcgacgccgc gatcgaggcc tcgggccttt ccggcaggcc
     4201 gaggccgggc ctccccgccg tcttctggga cgtcttcggc gagaagggcc atcccctgcg
     4261 cgcgaccgtc tcggagatgg ggcctctcct tctcgggcgg gccctcggcc tctcggagac
     4321 ccaggcggag gtcctctccg tggtcttccg cgtcgcggac gaccgcggcc tcctcctcct
     4381 cgacctcaag gacctgcggg ccgtcctcga gttctgctca agcaacgccc aggccctcga
     4441 gcccgactac ggccgcatgg cccccgcctc cctgggcgcg atccagcgcg gcatcgtcgt
     4501 cctccagggc cagggcggcg acctcttctt cggcgagccg gccctcgaca tcggcgagct
     4561 cctccggaag gacgagaagg gccgcggcct cgtccacgtc ctcgccgcgg accggctcct
     4621 cgaggccccc tccctctacg ccgccttcat gctctggctc ctctccgagc tcttcgagga
     4681 gctccccgag gagggggacc tgcccgcccc gaagctcgtc ttcttcttcg acgaggccca
     4741 cctcctcttc gacggggcgc ccgaggagct cgtgaggagc gtcgcccgca ccgtgcgcct
     4801 cgtgcgctcc aagggcgtcg gcgtctggtt cgtcacgcag aacccgacgg acctgccgga
     4861 ggaggtcctg gcccagctcg gcaaccggat ccagcacgcc ctgcgcgcct ataccgagaa
     4921 ggagcggcgc tcgctcaagg ccgccgccga ctccttcagg cccaatccca agctcgacgc
     4981 cgaggccacc cttggcgagc tcggcgtcgg cgaggccctg gtctccctcc tggac
//
LOCUS       DAOOSJ010000197         5025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10432_length_5025_cov_5.577867, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000197 DAOOSJ010000000
VERSION     DAOOSJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_5025_cov_5.577867"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLG14_09595"
     CDS             complement(<1..302)
                     /locus_tag="PLG14_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49423.1"
                     /translation="MRNKFVLAALALCLGSAAFAAPATYSAFIGANFFGYDYTALETG
                     VGVAIPLQEGLAFEAKAAYAVRPSAGDAFMAIPLQAGVRFAFGSGPLGFNCALGMEP"
     gene            complement(536..1663)
                     /locus_tag="PLG14_09600"
     CDS             complement(536..1663)
                     /locus_tag="PLG14_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49424.1"
                     /translation="MERLRRTWSVVRKELIQLRRDRKMRAMILVAPILQVLVLGYAAD
                     MDVRDVPLAVLDGDLSPRSRGLAAALVASTAFELVAAPASQAELESALERGKAEIGVV
                     IPPSFGRDLDAGRSPGVQVLVDGTRSNTASVAMAYATATIAAFGAKAAPRQSAHPASL
                     PPVEARSRVWYNPELKSRLFMVPGIFALLAMVFTIILTSLAVVKEKEIGTLEQLMVSP
                     LRRGELIAGKLIPFLLVSVVILGVALAAVRVFFGISPRGSLLLLALLSVVFAVSTLGV
                     GLFVSTIARTQHQAMIFSIFFFMFPMMILSGFVFPIANMPAPVRAVTRLMPLRYYFVI
                     VRGIFLKGSGMATLWPQLLPMAAIGAAVIAVSVARFKKQLD"
     gene            complement(1665..2798)
                     /locus_tag="PLG14_09605"
     CDS             complement(1665..2798)
                     /locus_tag="PLG14_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49425.1"
                     /translation="MNRRVKAIARKEFRHILRDPGSLGALLGLPLFLLLMFGYAVSLD
                     VKRVPLAVRDLDRSPESRALAAAILSAGDFVPGGTPDSPAAEAEALDSGRAAAVLEIP
                     RGFSALLAERKSASVQLLVDGSDGTRAQTVLGYLESAIRRFGEDRLVLEAARLPSGAS
                     RALAVDARPRVLFNQELRSVLFLVPGLVALILIITAVVSASLSVVREKETGSMEQILV
                     SPVGSFEFIVGKTLPYAAVGAVSAATVLAVAALVFGVAVKGSLLALAASTALFLLACL
                     GLGVLISTLAETQQVAFLVSTMATLIPTFTLSGFVFPLRNMPLAIRAAARLFPTTYYI
                     EILNAILVKGAGIAAYWRPAAALAAYAAATALASAARFARARR"
     gene            complement(2795..3724)
                     /locus_tag="PLG14_09610"
     CDS             complement(2795..3724)
                     /locus_tag="PLG14_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49426.1"
                     /translation="MSDAIVAEGLTKRFGGFLAVDGVSFAVPSGSVFGLLGANGAGKS
                     TTIRMLCGLLSPSGGRASVAGLDVASRPEEVKRSIGYMSQKFSLYEDLTARENVEFFA
                     GLYGIGRSARRRECDRVLEMAGLEGREDELSRELPGGFRQRLALGCALLHRPAVLFLD
                     EPTAGVDPVARRRFWDVIYGLTASGSTVLVTTHYLDEAEYCSTVTLMRDGRVVASGSP
                     GELKAERFPGLLLEIEHERPADVLAALSGAPGVADASLFGSRVHAGLAPGAGPEAALR
                     ALEAAGLGGARTASVRPSLEDVFIRVVSGKAEP"
     gene            complement(3721..>5025)
                     /locus_tag="PLG14_09615"
     CDS             complement(3721..>5025)
                     /locus_tag="PLG14_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX49427.1"
                     /translation="SLEGAEAALEAADARRDAASRLRLPSVLASAAYSHAGPVEPGVL
                     AIDLGAPAGVKSVALPEALQDSTSFRIVLQQPLYTGGRLASGLAQAEAGRAAALAETA
                     ARRRDVAAAAERAWWGLVLASESEAAVAENLLAVRSHRADAERRLAEGTATKSELLSW
                     RMQEVEASVRARSADADRAAARARLNVLLGRPWDAPLEAAEEPTMAADGSAAEPTRAA
                     AESTGAAAAAAPEGEASCPDGGAGESGLLATALGSRPELAAARARVEAREAAVGVARS
                     ALLPALHLTASAAYADPNPKAFPQRPGFELLWDIGLVASLDLGRLPFARSQVEEARAQ
                     AREARGALAQAQDGVALELALAGLELEKARDRLESSSAAVALAEENRRVQEERFAAGI
                     ALASELADAESALLLARLDGTRSRVAWELARAALRDAAGGTR"
BASE COUNT          775 a         1731 c         1933 g          586 t
ORIGIN      
        1 ggctccatgc cgagggcgca gttgaagccc agggggccac tcccgaaggc gaagcgcacg
       61 cccgcctgga gggggatggc catgaaggcg tcgcccgccg agggcctcac cgcgtaggcc
      121 gccttggcct cgaaggccag cccctcctgg agggggatcg cgacgccgac gcccgtctcc
      181 agggcggtat agtcgtaccc gaagaaatta gcgccgataa aggcgctgta ggtcgccggc
      241 gccgcgaagg cggcggagcc gaggcagagg gctagggcgg ccaggacgaa cttgttgcgc
      301 atgggtttcc tccgacgccg gacagcatag cacgaaacgc cctgacgacg aagcagcggc
      361 tccgaaagcg gcgcgcgtcc gagccggagg tatggcggag cgactggacg cgggggcgga
      421 aagagccgcg ggattcgccg tgtcggaaac cgccgggccg gccgagcccc cctcgcggcg
      481 aaaaacgcgc tcgtccttgc gcggccgccg ggcaggcctc gctcccggcc cttccctagt
      541 ccagctgctt cttgaaacgc gcgacgctga ccgcgatcac cgcggccccg atggcggcca
      601 tgggcaggag ctggggccag agcgtagcca tgcccgagcc cttgaggaag atgccgcgca
      661 cgatcacgaa gtagtagcgc agcggcatga ggcgggtgac ggccctgacc ggcgccggca
      721 tgttggcgat cgggaagacg aagccggaga ggatcatcat cgggaacatg aagaagaaga
      781 tggagaagat catggcctgg tgctgggtcc tggcgatggt cgagacgaag aggccgacgc
      841 cgagcgtgga gacggcgaag acgacggaga ggagggcgag gaggaggagg cttcccctcg
      901 gcgatatccc gaagaagacg cgcaccgcgg ccagggcgac gccgaggatg acgacgctga
      961 ccaggaggaa ggggatgagc ttccccgcga tgagctctcc gcgccgcagg gggctcacca
     1021 tgagctgttc gagggtgccg atctccttct ccttcacgac cgcgagcgag gtcaggatga
     1081 tcgtgaagac catcgcgagc agggcgaaga tgccgggcac catgaagagc cgcgacttca
     1141 gctcgggatt gtaccagacc cgcgacctgg cctcgaccgg gggcagggac gccggatggg
     1201 ccgactgccg cggcgcggcc ttcgccccga aggcggctat cgtcgccgtg gcgtaggcca
     1261 tcgccacgga agccgtgttg gaccgggtgc cgtccacgag gacctggacg ccgggcgagc
     1321 gccccgcgtc caggtcgcgg ccgaaggagg gcgggatgac gacgccgatc tccgccttgc
     1381 ccctctcgag cgcggactcg agctccgcct gcgacgcggg ggcggcgacg agctcgaagg
     1441 ccgtggaggc gacgagggcg gcggcgaggc cgcggctccg cgggctcagg tccccgtcca
     1501 ggacggccag gggaacgtcg cgcacgtcca tgtccgccgc gtagccgagc acgaggacct
     1561 gcaggatcgg cgcgaccagg atcatcgcgc gcatcttcct gtcccggcgg agctggatga
     1621 gctccttcct cacgacgctc caggtcctcc gcaggcgttc catctcatct cctcgcccgc
     1681 gcgaagcggg ccgcgctcgc cagggccgtc gccgccgcgt aggcggcgag ggccgcggcg
     1741 ggccgccagt aggcggcgat ccccgcgccc ttcacgagga tggcgttgag gatctctatg
     1801 tagtaggtcg tcgggaagag cctcgccgcg gcgcggatgg cgaggggcat gttgcgcagg
     1861 gggaacacga agccggagag cgtgaaggtc gggatgaggg tggccatggt gctgacgagg
     1921 aaggccacct gctgggtctc cgccagcgtg gagatgagga cgcccaggcc caggcaggcg
     1981 aggaggaaaa gggccgtcga cgccgcgagg gcgaggaggc tgcccttcac cgcgacgccg
     2041 aagaccaggg ccgcgaccgc caggaccgtc gccgcggaga cggcgcccac ggccgcgtag
     2101 ggcagagtct tgccgacgat gaactcgaac gagcccaccg gcgagacgag gatctgctcc
     2161 atcgagccgg tctccttctc ccgcaccacg gacagggagg ccgagacgac ggccgtgatg
     2221 atgaggatga gggcgacgag gccgggcacc aggaagagca cgctcctgag ctcctggttg
     2281 aagaggaccc ggggcctcgc gtcgacggcc agggcccgcg aggctcccga cggaagcctc
     2341 gccgcctcga ggacgaggcg gtcctcgccg aagcggcgga tggccgactc gaggtagccc
     2401 aggaccgtct gcgccctcgt cccgtcgctt ccgtccacga ggagctggac ggaagcgctc
     2461 ttcctctccg cgaggagggc ggagaagccg cggggtatct cgaggacggc tgcggccctc
     2521 cccgagtcga gggcctccgc ctcggcggcc ggcgagtcgg gcgtcccgcc agggacgaag
     2581 tccccggcgg agaggatggc ggccgcgagg gccctgctct cgggactccg gtccaggtcg
     2641 cggaccgcga gggggacgcg cttcacgtcc aggctcacgg cgtagccgaa catgaggagg
     2701 aggaagagcg ggaggccgag cagggccccg aggctccctg gatcgcggag gatatggcgg
     2761 aactccttgc gggcgatggc cttgacccgg cggttcacgg ctccgccttc ccggagacga
     2821 cgcggatgaa gacgtcctcc agcgaggggc ggacggacgc cgtcctcgcg ccgccaagtc
     2881 cggcggcctc cagggcccgc aaggccgcct cggggccggc cccaggggcg aggcccgcgt
     2941 ggacgcggga gccgaagagc gaggcgtccg cgacgcccgg cgcgcccgag agggcggcca
     3001 ggacgtcggc cggcctctcg tgctcgatct cgaggaggag gccggggaag cgctcggcct
     3061 tgagctcgcc gggactgccc gaggccacga cgcggccgtc gcgcatgagg gtgacggtcg
     3121 agcagtactc ggcctcgtcg agatagtggg tggtcacgag gacggtggag cccgaggccg
     3181 tgagcccgta gatgacgtcc cagaaccgcc ggcgcgccac cgggtccacg ccggccgtcg
     3241 gctcgtcgag gaagaggacg gcgggccggt gcaggagggc gcagccgagg gcgaggcgct
     3301 ggcggaagcc ccccgggagc tcccgggaaa gctcgtcctc ccggccctcg agtccggcca
     3361 tctcgagcac ccggtcgcat tcgcgcctcc gcgcggaccg gccgatgcca tagaggccgg
     3421 cgaagaactc gacgttctcc ctggccgtga ggtcctcgta gagggagaat ttctgggaca
     3481 tgtagccgat gctccgcttg acctcctcgg ggcgggaagc cacgtcgagg cccgccacgc
     3541 tggccctgcc cccgctcggc gaaaggaggc cgcagagcat cctgatcgtc gtggacttgc
     3601 ccgcgccgtt cgcgccgagg agtccgaaga ccgagcccga gggcaccgcg aagctgacgc
     3661 cgtcgacggc gaggaatccg ccgaagcgct tcgtgagccc ctcagcgact atggcgtcgc
     3721 tcatcgcgtc ccaccggccg cgtcgcggag ggccgccctc gcgagctccc aggcgacgcg
     3781 ggagcgcgtc ccgtccagcc gggccaggag gagggccgac tcggcgtcgg cgagctcgga
     3841 ggcgagggct atccccgcgg cgaatcgctc ctcctggacc cggcggttct cctcggcgag
     3901 cgcgaccgcc gccgaggacg actcgaggcg gtcgcgcgcc ttctcgagct cgaggccggc
     3961 gagggcgagc tcgagggcga cgccgtcctg agcctgggcg agggctccgc gcgcctcgcg
     4021 ggcctgcgcc cgggcctcct cgacctgcga gcgggcgaag ggcagcctgc ccaggtccag
     4081 gctcgcgacg aggccgatgt cccagaggag ctcgaagccc gggcgctggg gaaaggcctt
     4141 ggggttggga tcggcgtagg ccgcgctggc ggtcaggtgg agcgcgggca ggagggccga
     4201 gcgcgcaacg ccgacggccg cctccctcgc ctcgaccctc gccctggccg cggcgagctc
     4261 gggacgggag ccaagggctg tggcgagaag gccggattcc cctgcacctc cgtcggggca
     4321 ggaggcttcg ccctcggggg cggccgccgc ggccgccccg gtcgactccg cggccgcccg
     4381 tgtcggctcc gcggctgacc cgtccgccgc catcgtcggc tcctcggccg cctccagcgg
     4441 cgcgtcccat gggcggccga gaaggacatt gagacgggcc cgcgcggccg cgcggtccgc
     4501 gtcggccgac cgcgccctga cggaggcctc gacctcctgc atccgccagg agaggagctc
     4561 gcttttcgtc gccgttccct cggcgaggcg ccgctcggcg tcggcgcgat gggaccgcac
     4621 cgccaggagg ttctcggcga ccgccgcctc gctctccgag gcgaggacaa ggccccacca
     4681 ggcgcgttcg gcggcggcgg cgacgtcgcg ccgcctggcc gcggtctccg caagggccgc
     4741 cgccctgccc gcttcggcct gggccaggcc cgaggccagg cggccgcccg tgtagagggg
     4801 ctgctggagg acgatgcgga agctcgtcga gtcctggagg gcttcgggga gagcgacgga
     4861 cttcacgccg gccggagccc caagatcgat ggcgaggacc cccggctcca ccggcccggc
     4921 atgcgagtac gccgccgaag ccaggacgct cggcagcctg aggcgcgagg cggcgtcccg
     4981 cctcgcgtcc gcggcctcga gggccgcctc ggcgccctcg aggga
//
LOCUS       DAOOSJ010000198         5002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10490_length_5002_cov_6.974732, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000198 DAOOSJ010000000
VERSION     DAOOSJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10490_length_5002_cov_6.974732"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLG14_09620"
     CDS             complement(<1..1091)
                     /locus_tag="PLG14_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX49428.1"
                     /translation="MLDSSLPALAGKIALGLLASVVGLGLLGLALLLAASPGRARPVL
                     GADGRPLPGSISEKVFITVNGSRQGIFLKGKDRTKPVLLYLHGGMPDYFLTERYPTGL
                     EELFVVAWWEQRGSGISYDPAAAERPVTLDLMVKDTLAVADWLRARFGAEKVYLMGHS
                     GGSFVGIHAAARAPEKFAAYVAVAQMAEPRKSEALAYEYMLREYRERGDARMVRRLER
                     FPVGAAGDFPAGYAMTRDVAMHDLGVGTTRGMRSIARGLLLPSLAFPEYGAREKWRFW
                     AAKARSGIASLWGDMLSAELPRSLPRLELPVYFLHGAHDYTCSYPLAKAYLQALEAPA
                     KGFYTFPEAAHSPLFEDPARLREILGKDVL"
     gene            1176..>1762
                     /locus_tag="PLG14_09625"
     CDS             1176..>1762
                     /locus_tag="PLG14_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49429.1"
                     /translation="MARPKTNFDPTREALLRTACRLFSERGYEETPISLIMSEAGLSK
                     AGMYHYFASKEEILEEAIGLYVQRGVERLRAASAGKPLEERLVHFILGAGRRDGIERG
                     LEAMKGSSPGSYAAYRIRERSLHADIPLLAEILEEGMELGLYRRAGSPRAVASVLVLQ
                     AKALAEGNYLPPAGPEERLERLEAYLDLLRTWLDPP"
     assembly_gap    1763..1862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1863..3845
                     /locus_tag="PLG14_09630"
     CDS             <1863..3845
                     /locus_tag="PLG14_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOX49430.1"
                     /translation="LALAALLLSGGLAAAQDLPVTKVALFSSGVGYFERRGTVKGEAT
                     VSLPFSTQEVDDVLKSLVVWDLAGGGAGPSVSYPSLETLDEALRGLGVDLRGNPRVEE
                     LLARLRGAELVVEAPERIQGRVVSVEQRPTGKDGSPRATVVLLTQGGVRAVALDDLAS
                     FRFADPAVAADFERGLALILGARDAERRVLDLRLPGTGSREAALGYVVAAPVWKASYR
                     LDLSGDKPWFQGWAVVDNPSQSDWKDVSLTLVSGRPVSFIQNLYAPLRLARPVLPLSV
                     AGTAEALTYGSGLEPEAYYELDEMAAAPPAARALAAPSREKKAEYSGAAADRLSLAAS
                     AAGAAEARAAGDQFEFTIRKPVTLERRRSAMLPLVAGAMAAEKVSVWRPGSGSKNPML
                     GARIVNSLGMKLPAGPVTVFDGGAYAGDALLEFLPEKDKRLVAYGQDLSVEVEDSSAQ
                     SRETTGVTVAKGVLVFSRRVTYTRTYVAKNASASPRKLLVEHPVTAGAELAAPASFEE
                     KTGSAYRFLVSLPAGGQAKLEVKERSPAQERVVLAGLSSESFLAYASSSEIPAKVKEA
                     LRKAIELKGKAEDAKKALADLEARSAELESGQSRIRSNLSAVGRDSTQGQQYLKRLMD
                     SEAELDGLEARIEEARKASREAQKAYEGYLGSLSLE"
     gene            4093..>5002
                     /locus_tag="PLG14_09635"
     CDS             4093..>5002
                     /locus_tag="PLG14_09635"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX49431.1"
                     /translation="MAGFFAAAYAFMAGAVAFSIAVTLRSEDSASPLAKSFLYFSLGT
                     LVWNAAYAAMTLSTDESFVRLVYRASSLGWGANPSLALYYAVGLYDEHVARIRSRRLR
                     LLVFAPIPLIWYGVFAGKILAVGFERGPNGWIERMDPSSPWNWAFVALMLVGLGIFFY
                     IGLELARRSRTNRARLHARLITFPVSLMIVLVIVFHIVLPLAGLQKFPPTAQLLLALW
                     LVVVSAKLQRYPVFSLSPRIAADAIVGSMSDMCFLVDPDGLVSGANEAVLGLLGRGPA
                     SLAGRAASEVLSPEVATLLGGAAKGTG"
BASE COUNT          677 a         1780 c         1725 g          720 t
ORIGIN      
        1 aggacgtcct ttccgaggat ctcgcggagc cgggcagggt cctcgaagag ggggctgtgg
       61 gccgcctcgg ggaaggtgta gaagcccttc gccggcgcct cgagagcctg gaggtaggcc
      121 ttggcgaggg gataggagca ggtgtagtcg tgggcgccgt ggaggaagta gacggggagt
      181 tcgaggcggg ggaggctccg cgggagctcc gccgagagca tatcgcccca gagcgacgcg
      241 atgcccgacc tcgccttggc ggcccagaag cgccacttct cccgggcgcc gtactccggg
      301 aaggcgaggg aggggaggag gaggcccctc gcgatggagc gcatgccgcg cgtcgtgccg
      361 accccgaggt cgtgcatcgc gacgtccctg gtcatcgcgt agcccgccgg gaagtccccc
      421 gccgcgccca cggggaagcg ctcgagcctc cggaccatcc gcgcgtcgcc ccgctcccgg
      481 tattcgcgca gcatgtactc gtaggccagg gcctccgact tccggggctc ggccatctgg
      541 gccaccgcga cgtaggcggc gaatttctcc ggcgcccgcg cggccgcgtg gatgccgacg
      601 aagctgccgc ccgagtgtcc catgaggtag accttctcgg cgccgaagcg ggcgcggagc
      661 cagtcggcga ccgcgagggt gtccttgacc atgaggtcga gggtgacggg ccgctccgcc
      721 gcggcgggat cgtaggagat gccggagccc cgctgttccc accaggcgac gacgaagagc
      781 tcctcgaggc cggtcgggta tcgctccgtg aggaagtagt cgggcatgcc gccgtggaga
      841 tagagaagga cgggcttcgt gcggtccttg cctttgagga agattccctg ccgtgagccg
      901 ttgaccgtga tgaagacctt ctccgagatc gagccgggca ggggcctgcc gtccgctccc
      961 aggacgggcc tggcccggcc ggggctcgcg gcgaggagga gggcgaggcc cagaaggccc
     1021 aggccgacga ccgacgcgag gaggccgagg gctatcttgc ccgcgagggc cggaaggcta
     1081 gaatccagca taaggtctcc ttgacagcgc gtacgccggg tggtaatttg accgtggtta
     1141 tttaaccacg gtcaatatat cgccaaggat gagccatggc aaggcccaag acgaatttcg
     1201 atccgacccg cgaggcgctc ttaaggacgg cctgccgcct cttctccgag cgcggctacg
     1261 aggagacgcc gatcagcctc atcatgagcg aggcgggcct gagcaaggcg ggcatgtacc
     1321 actacttcgc ctccaaggag gagatcctgg aggaggcgat cggcctctat gtccagcgcg
     1381 gcgtcgagcg cctgcgggcg gcctccgcgg ggaagccgct cgaggagcgg ctcgtccact
     1441 tcatcctggg cgccgggcgg cgggacggga tcgagcgcgg cctggaggcg atgaagggct
     1501 ccagccccgg ttcctacgcg gcctaccgga tccgggagcg cagtctccac gcggacattc
     1561 ccctcctcgc ggagatcctc gaggagggca tggagctcgg cctctaccgc cgggccggaa
     1621 gcccgcgcgc cgtcgcctcg gtcctggtgc tccaggccaa ggccctcgcg gagggcaact
     1681 acctcccccc ggcgggaccg gaggagcggc tggagcggct ggaggcctac ctcgacctcc
     1741 tgcggacctg gctcgatccg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnctcgccct cgccgccctt ctcctttcgg gagggctggc cgccgcccag gacctgcccg
     1921 tcacgaaggt cgcactcttc tcctcggggg tgggctattt cgagcgccgc gggacggtga
     1981 agggggaggc gacggtctcc ctgcccttct ccacgcagga ggtggacgac gtcctcaagt
     2041 ccctcgtggt ctgggacctc gccggcggcg gcgccggccc ctcggtctcc tatccctcgc
     2101 tggagaccct ggacgaggcg ctccgcggcc tgggcgtgga cctgcgcggg aatccgcggg
     2161 tcgaggagct cctggcgcgg ctccgcggcg ccgagctcgt cgtggaggcg cccgagcgca
     2221 tccaaggccg cgtcgtctcc gtcgagcagc ggcccacggg caaggacggc tcgccccgcg
     2281 cgacggtcgt cctcctgacc cagggaggcg tgcgcgccgt cgccctggac gacctggcga
     2341 gcttccgctt cgccgacccg gccgtggccg ccgacttcga gcgcggcctc gcgctgatac
     2401 tgggcgcgag ggacgcggag cggcgcgttc tcgacctgcg cctgcccgga acggggagcc
     2461 gcgaggcggc cctgggctac gtcgtcgccg cgccggtctg gaaggcctcg taccgcctgg
     2521 acctctcggg cgacaagccc tggttccagg gctgggccgt cgtcgacaac ccctcgcagt
     2581 ccgactggaa ggacgtcagc ctcaccctcg tgagcgggcg gccggtctcc ttcatccaga
     2641 acctgtacgc gcccttgcgg ctcgcgaggc cggtcctccc cctctccgtc gcggggacgg
     2701 ccgaggccct gacctacggc tcgggcctcg agcccgaggc ctactacgag ctcgacgaga
     2761 tggccgccgc tcctcccgcc gcgcgcgcgc tggccgcgcc ctccagggag aagaaggccg
     2821 aatactcggg cgcggcggcc gacaggctct ccctcgccgc ctccgccgcc ggcgcggccg
     2881 aggcccgggc cgccggcgac cagttcgagt tcacgatccg gaagcccgtg accctggagc
     2941 ggcggcgctc cgcgatgctg cccctggtcg ccggcgccat ggccgcggag aaggtctccg
     3001 tctggaggcc cggctcgggc tcgaagaacc cgatgctcgg cgcgaggatc gtgaacagcc
     3061 tggggatgaa gctgcccgca gggccggtca ccgtcttcga cggcggcgcc tacgcgggcg
     3121 acgccctcct cgagttcctt cccgagaagg acaagcggct cgtcgcctac ggccaggacc
     3181 tctccgtcga ggtcgaggac tcgagcgcgc agagccggga gacgaccggc gtgaccgtcg
     3241 cgaagggcgt cctcgtcttc tctcggcggg tgacctacac gaggacctac gtcgccaaga
     3301 acgcctccgc ctcgccgcgg aagctcctcg tcgagcaccc cgtcacggcc ggcgccgagc
     3361 tcgccgcgcc cgcctccttc gaggagaaga ccggttccgc ctaccgcttc ctcgtctccc
     3421 tgcccgcggg cggccaggcg aagctcgagg tgaaggagag gagccccgcc caggagcgcg
     3481 tcgtcctcgc gggcctctcc tccgaaagct tcctcgccta cgcctcctcc tccgagatcc
     3541 cggccaaggt caaggaggcg ctcaggaagg ccatcgagct caaggggaag gccgaggacg
     3601 cgaagaaggc cctggccgac ctggaggccc gctcggccga gctcgagtcc ggccagtcgc
     3661 ggatcaggtc caacctctcc gccgtgggac gggactcgac ccagggccag cagtacctga
     3721 agcgcctcat ggactccgag gccgagctcg acggcctcga ggccaggatc gaggaggcga
     3781 ggaaggcgag ccgcgaggcg cagaaggcct acgagggcta cctcggctcg ctcagcctgg
     3841 agtagggagg ggccgcggcc gccttgaggc gcagcggccg actggagggg ccgcggccgc
     3901 gccgcgcccc gccgggccgt gatggccgcc gtgaccgcct tgccgaagcc gcaccgccat
     3961 ggccgcgccg cgccccgctg ggccgtgatg gccgccgtga ccgccccgcc gaagccgcgc
     4021 cgccgcggcc gcgcgaggtt gcggcgggga aggccatgca ttagcctcta acctaaggaa
     4081 ggggcgaaag ggatggcagg attcttcgcc gcggcctacg ccttcatggc gggggccgtc
     4141 gcgttcagca tcgcggtgac gctgcgctcc gaggactcgg cgtcgcctct cgccaagtcc
     4201 ttcctctact tctcgctcgg aaccctggta tggaacgcgg cctacgctgc gatgacgctc
     4261 tcgacggacg agtccttcgt ccgcctcgtc taccgggcga gctccctggg ctggggcgcc
     4321 aacccctccc tcgccctcta ctacgccgtc gggctctacg acgagcatgt cgcgcggatc
     4381 cgctcgcgcc ggctccgcct cctggtcttc gcgccgatcc ccctcatctg gtacggcgtc
     4441 ttcgccggca agatcctggc cgtgggcttc gagcgcggcc cgaacggctg gatcgagcgc
     4501 atggatccct cctcgccctg gaactgggct ttcgtcgccc tgatgctcgt cggcctcggg
     4561 atcttcttct acatcgggct cgagctcgcc aggcggtcca ggacgaaccg ggccaggctc
     4621 cacgccaggc tcatcacttt ccccgtctcg ctgatgatcg tcctcgtcat cgtcttccac
     4681 atcgtccttc ccctggccgg cctgcagaag ttcccgccca ccgcccagct cctcctcgcg
     4741 ctctggctcg tcgtcgtctc cgccaagctg cagcgctacc cggtcttctc cctctccccg
     4801 cggatcgccg ccgacgcgat agtcggcagc atgtccgaca tgtgcttcct cgtggacccg
     4861 gacggcctcg tctccggggc gaacgaggcc gtcctgggcc tccttggccg cggtcccgcc
     4921 tccctggccg gccgcgccgc ctcggaggtc ctctctcccg aggtcgcgac gctgctgggg
     4981 ggcgcggcca aggggacggg cg
//
LOCUS       DAOOSJ010000199         4986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10531_length_4986_cov_5.180491, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000199 DAOOSJ010000000
VERSION     DAOOSJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10531_length_4986_cov_5.180491"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLG14_09640"
     CDS             <1..357
                     /locus_tag="PLG14_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOX49432.1"
                     /translation="AIQDLSASKKIVLVPVDGEVASKLMAKWSFYTSTVIPAGTYQNV
                     AADVTTVSVKSMLAVSDKLDDKTVYEMLKTMFANGERLAASHKKGADVKLATATDGMS
                     IPLHPGAEKFYKENKK"
     gene            513..908
                     /locus_tag="PLG14_09645"
     CDS             513..908
                     /locus_tag="PLG14_09645"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOX49433.1"
                     /translation="MLSVRDPRGRELAAVDLPDGRFSHVFVHSFHLTPVEERIELVPG
                     GFRLYELRYESCGVGMPADAEGGYRLEDGVFVLDMDRRFKKIPIMVSVLPGHGIIAGD
                     AYLPFRNWARPEDTLVLQPRTKTTLRFRR"
     gene            915..2918
                     /locus_tag="PLG14_09650"
     CDS             915..2918
                     /locus_tag="PLG14_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOX49434.1"
                     /translation="MSAKEKNPAAGDPSIDAQAILKKFDKESDYRTLTGLAGKLVAAF
                     AISFTVFQLYTAVFGVFDAMIQRSIHLAFGLSLIFLLYPTSKKWRRDRIHPLDGALAV
                     LGALAPLYIVVFYQALVARSGTATPLDIAFGIVGILLVLEAARRVVGVPIVVIAVVFV
                     AYAFAGPYIPGTLGHRGADFETLVQHLYFTTEGVFGIPIGVSSTFIFLFILFGAYLEK
                     TGLGQLFIDLANAVAGWAAGGPAKVAVLSSALMGTVSGSSVANVVGTGSFTIPMMKKL
                     GYKPEFAGAVEATASTGGQLMPPIMGAAAFLMAEFTNIPYRRIIGAAAIPALLYYFGV
                     WAGVHFEAKKLGLKGIPREELPKFKRIMLDRGHLIIPLIAIIYLLVAGFTPMKAALWA
                     IILSIVASWLRKSTRISLKDIVKGLESGARGALGIIAATACAGIIIGVVTQTGLGLKL
                     GSSLVQAAGGNLLLTLFFTMITSLVLGMGVPTTANYVITSTITAPAVIMILMNQSGLA
                     AGSAIPASIILPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPMKTGFAASKLAIA
                     AFLVPYIFVLNPQMLLFNIKPLEMIWMIATSLVGIISVAAGVGAFLKANMSWLERILF
                     FVGGVLLVTPGLLTDAIGGGLILIGVVIQYRKARLGGAAKAAA"
     gene            3090..4382
                     /locus_tag="PLG14_09655"
     CDS             3090..4382
                     /locus_tag="PLG14_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49435.1"
                     /translation="MAPRQRATRRPSPALLLGLAALLASAAPALAEAQAALPSPAPGG
                     RAVPAVAILEMANAGLDPRLDYLAGIIQGLLAYDLGGRSDLVLVDRRNLEAVLKEKEL
                     ALSPLGSGSSGGGEASTEELSRAGRLAGADWLLSGEYAFLGADVLVTLSLTETASARR
                     TVFRDRGPSENLVHALAEKVVERLTGTRPSFVEAGRERSLVSLRDETPGSLALHSPII
                     DAEIFLDGEFIGFTTGDERKPFLVERLAPGKHRVRTHLDSSFGVLKLPEVAFGDWDVE
                     VVVEPGKRLVLRDPTRHFNDQLYRLIRLWDEEYTAPFGQGAKLAVLKELAFQDRQGSP
                     VSVRLEARPLSDTAASPEAATGAVAMEFVLTVKAGPGPAESATLRLERDLAAGSESEG
                     EANAGIVRLSVSLERGYREWELELSLERTDIRQNMWRE"
BASE COUNT          711 a         1926 c         1559 g          790 t
ORIGIN      
        1 gccatccagg acctctccgc ctccaagaag atcgtcctcg tgcccgtgga cggcgaggtc
       61 gcctccaagc tcatggccaa gtggagcttc tacacctcca ccgtcatccc cgccggcacc
      121 taccagaacg tggccgccga cgtcaccacc gtctccgtga agtcgatgct cgccgtcagc
      181 gacaagctcg acgacaagac cgtctacgag atgctcaaga ccatgttcgc caacggcgag
      241 cgcctcgccg cctcccacaa gaagggcgcg gacgtgaagc tcgcgaccgc gaccgacggc
      301 atgtcgatcc ccctgcaccc gggcgccgag aagttctaca aggagaacaa gaaatagctt
      361 ccgcgagccc ctccgccggc caggcggagg gctcgggccc caggccgggc cgcgccggga
      421 ggctctcccg cgccgcggcc gccggcctcg tcctcctcgc cgcggccgcc gtcggggcct
      481 atgtcctcgg cgtccggccg gagaggacgg caatgctttc cgtcagggac ccgcgcgggc
      541 gcgagctcgc cgcggtcgac ctccccgacg gccgtttttc ccacgtgttc gtccactcct
      601 tccacctcac gcccgtggag gagaggatcg agctcgtccc gggcggcttc cgcctctacg
      661 agctccgcta cgaaagctgc ggcgtgggca tgccggccga cgcggaaggc ggctacaggc
      721 tcgaggacgg cgtcttcgtc ctcgacatgg accggcgatt caaaaagatc cccatcatgg
      781 tttccgtctt gccgggtcac ggcatcatcg cgggggacgc atacctccct ttccgcaact
      841 gggcccgtcc agaagatacg ctcgtcctcc agccgaggac gaagacgaca ctacgtttcc
      901 ggaggtaagc tcgcatgagc gccaaagaga agaacccggc cgcaggcgac ccgtccatcg
      961 acgcccaggc catcctcaag aagttcgaca aggaatccga ctacaggacc ctcacgggcc
     1021 tggcgggcaa gctcgtcgcg gccttcgcga tcagcttcac cgtcttccag ctctacacgg
     1081 cggtcttcgg cgtcttcgac gcgatgatcc agcgctcgat ccacctcgcc ttcggcctct
     1141 ccctgatctt cctcctctac cccacgagca agaagtggcg cagggacagg atccatcccc
     1201 tggacggcgc gctcgccgtc ctcggcgccc tggcgccgct ctacatcgtc gtgttctacc
     1261 aggccctggt ggcccgctcc ggcacggcga cgcccctcga catcgccttc ggcatcgtcg
     1321 gcatcctcct cgtcctggag gcggcccgcc gcgtcgtcgg cgtgcccatc gtcgtgatcg
     1381 ccgtcgtctt cgtcgcctac gccttcgccg gcccctacat ccccggcacc ctgggccacc
     1441 gcggcgcgga cttcgagacc ctcgtccagc acctctactt caccacggag ggcgtcttcg
     1501 gcatcccgat cggcgtgtcg agcaccttca tcttcctctt catcctcttc ggcgcctacc
     1561 tggagaagac cggcctgggc cagctcttca tcgacctcgc gaacgccgtc gcgggctggg
     1621 ccgccggcgg acccgccaag gtcgccgtcc tctccagcgc cctcatgggc acggtctccg
     1681 gctcctccgt cgccaacgtc gtcggcacgg gcagcttcac gatccccatg atgaagaagc
     1741 tcggctacaa gcccgagttc gccggcgccg tcgaggccac cgcctccacg ggcggccagc
     1801 tcatgccccc gatcatggga gccgccgcct tcctcatggc ggagttcacg aacatcccct
     1861 accgccgcat catcggcgcg gccgccatcc ccgccctcct ctactacttc ggcgtctggg
     1921 ccggcgtcca cttcgaggcg aagaagctcg gcctcaaggg catcccgcgc gaggagctgc
     1981 cgaagttcaa gcgcatcatg ctcgaccgcg gccacctcat catccccctc atcgcgatca
     2041 tctacctcct ggtcgcgggc ttcacgccga tgaaggccgc cctctgggcc atcatcctct
     2101 ccatcgtcgc gtcctggctc aggaagagca cccgcatcag cctcaaggac atcgtgaagg
     2161 gcctcgagtc cggcgcgcgc ggcgccctgg gcatcatcgc cgcgacggcc tgcgccggca
     2221 tcatcatcgg cgtcgtcacc cagacgggcc tcgggctcaa gctcggctcc agcctcgtcc
     2281 aggccgccgg cgggaacctc ctcctcaccc tcttcttcac gatgatcacc tccctggtcc
     2341 tgggcatggg cgtcccgacc accgccaact acgtcatcac ctccacgatc acggcgccgg
     2401 ccgtcatcat gatcctcatg aaccagtccg gcctcgcggc gggctccgcc atccccgcct
     2461 ccatcatcct ccccgcccac atgttcgcct tctacttcgg catcatcgcc gacgtgaccc
     2521 cgccggtggc cctagcggcc ttcgccggcg cgggcatcgc gaaggccaac ccgatgaaga
     2581 cgggcttcgc cgcctccaag ctcgcgatcg ccgccttcct cgtgccctac atcttcgtcc
     2641 tcaatcccca gatgctcctc ttcaacatca agcccctgga gatgatctgg atgatcgcga
     2701 cgagcctcgt gggcatcatc tccgtcgcgg ccggcgtcgg cgccttcctc aaggcgaaca
     2761 tgagctggct cgagcgcatc ctcttcttcg tgggcggcgt cctcctcgtc acccccggcc
     2821 tcctcaccga tgccatcggc ggcggcctca tcctcatcgg cgtcgtcatc cagtacagga
     2881 aggcgaggct cggcggagcc gccaaggcgg cggcctagcc cggccggcgg agggccgagg
     2941 gccttccgcc gaccggccgc cagggcccct ctcaagggcg ccctgtctcg aggggctgtc
     3001 ccgaaggggc agcccctcct cgtttcgccc ccgccgtccg agcggagacg gcggttgatc
     3061 ggccgcgcgc cctgcgcgat actgtccgca tggcacccag acaacgcgcg acccgacgcc
     3121 cctcccccgc cctcctcctt ggcctcgcgg cccttctcgc ctccgccgct ccggccctcg
     3181 ccgaggccca ggccgccctt ccctcccccg ccccgggcgg cagggcggtt cccgccgtcg
     3241 ccatcctgga gatggcgaac gcgggcctcg acccccgcct cgactacctt gcgggtatca
     3301 tccagggcct cctcgcctac gacctggggg gaaggagcga cctcgtcctc gtggacaggc
     3361 gcaacctgga ggccgtcctc aaggagaagg agctcgccct gagccccctg ggctcgggct
     3421 cttccggcgg cggcgaagca tcgaccgagg agctctcgcg ggccgggcgc ctcgcgggcg
     3481 cggactggct cctctcgggc gagtacgcct tcctgggcgc cgacgtcctg gtcaccctct
     3541 ccctcacgga gacggcgagc gcgaggcgca cggtcttccg ggaccgcggc ccgagcgaga
     3601 acctcgtcca cgccctcgcg gagaaggtgg tcgagcggct cacgggaacc cgcccatcct
     3661 tcgtcgaggc cggacgcgag cgctccctcg tctccctgcg cgacgagacg ccgggcagcc
     3721 tcgccctcca ctcccccatc atcgacgcgg agatcttcct ggacggtgag ttcatcggct
     3781 tcacgacggg cgacgagcgg aagcccttcc tggtcgagcg cctcgcgccc gggaagcatc
     3841 gcgtccgcac acacctcgac tcctccttcg gcgtcctcaa gctcccggag gtggccttcg
     3901 gcgactggga cgtggaggtg gtcgtggagc cggggaagcg cctcgtgctg cgcgatccca
     3961 ccaggcactt caacgaccag ctctaccgcc tgatccggct ctgggacgag gagtacacgg
     4021 cgcccttcgg ccagggcgcg aagctcgccg tcctgaagga gctcgccttc caggacaggc
     4081 agggctcgcc cgtctccgtg cgcctcgagg cgcggcccct ctccgacacg gcggcctcgc
     4141 ccgaggcggc cacgggcgcg gtcgccatgg agttcgtcct cacggtgaag gccggcccag
     4201 gcccggcgga gagcgcgacg ctcaggctcg agcgcgacct tgccgccggc tccgagtccg
     4261 agggcgaggc gaacgcgggt atcgtgaggc tctcagtctc cctggagcgg ggctacaggg
     4321 agtgggagct cgaattgtcc ctcgagcgca cggacatacg gcagaacatg tggcgggaat
     4381 aggggagacg cgcggcggga ttcgtaagga tctgctggac gggtcctgag gaggggaaag
     4441 gggctccccc tctccgctgc gcttcgaggg gagccccttt cccctcctca cccgtcgtcc
     4501 cgccccgcgt caagaaccgc gggcgggttt gatcgccttc acgccgcgag ccttgcccac
     4561 gggctgaggt tcggggcggc gctcttcgcg gttaggttgg gcgcggcggc ttgggtgcgc
     4621 cgcgaagcgt tgagggcgca acttcgtctc gcgaggctcg cgcgcctgcg gcgcggcgca
     4681 gtcagggacg gccctttggc gtgcgccgcc tggtgacgcg gggcacgagc gctacgcgcg
     4741 gcgcctacgg ggtgggttcc ttggagtacg ccgcctgggg ccgaagagca cgcgcgctgc
     4801 gcgcggcgcc tacagggtcg gctccttgga gcgcgccgcc tggtgacgcg gggcacacgc
     4861 gctgcgcgcg gcgccggcaa gtgaggcttg cggatggcgt tgcctttgcc cgcagcaacg
     4921 cgcgccttcg gcgcggcgcc gatgactctc ccatgggcga cgccgaccct ccagagggtg
     4981 gcgacg
//
LOCUS       DAOOSJ010000200         4981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10549_length_4981_cov_4.034308, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000200 DAOOSJ010000000
VERSION     DAOOSJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10549_length_4981_cov_4.034308"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3110)
                     /locus_tag="PLG14_09660"
     CDS             complement(<1..3110)
                     /locus_tag="PLG14_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49436.1"
                     /translation="MATLRRAPAALSGVILAGGLAALLLLPSLDRDLRDRLSGALQGL
                     QASVERETGLRLTFESMSPSILKAFSLRRIELKDAEGRTVLAAERVYAYYDFGALLLG
                     KGQLAISELSFARVRADLRRADLERLSALASRFGGGGGGGGSKGLPSFAVSGKSLDLR
                     LSGFAPGELRAEIDSFRIGKGGPDAQLLLSGRLSARVDPALAPGIGRLEVPVGVDGLV
                     ARDFSRARLMLSLKASSDSFALERQRFELSYAQGVAELRKVEDEAPLDAFVRYDLRGR
                     ALAASLRMEGYAPARMATLRGGLSSLSPWLRIPLSGSLSFEALAGDPKRTRYSLELSG
                     ELADSSLGSLARAAVSAKGGPEGADIAYARVEGGLGRASYSGSLGFRGLSLDGELALE
                     ASLAEGRLPLEADLRVLGGQGHYAAYSDSVRAAGVAFRDFALDASIGSGGAGSLDFGL
                     SFRPPEAEAPPILALPPDEAAPAFSGEAVAASGGSPRFRCEGSLSGGPEPVLELSLGL
                     ESFDLAAVEPLAAALVSSGAAALLSDLRLDGEVFALSDLKRLSFSTTDFALVSRSSPG
                     AYALVSVSGSEKGVSLRKASVSYGGYRAEASGSADFAEAGRLGFEAKLSLEDVPYSVK
                     GTYAGEGLFLSGDYGLEVKLRGMGGETYLEARADGLPVPLKGATLVVGLDADGRYAGP
                     ADWSLLLSSLSAELAGSAGAGLPRLGLSGTLGPGGGDFPSISLSDAESSLSGSGRLDY
                     DLSRSSRSASFKGGLSGKGGERYDLAASLSGDSLEARFEVASSPVSRFVKGPLSGRVD
                     GKARVSGSIERPLLSFEASLRDGALAEDPLELVLSGSFGDGLLRVGKARAAWRGMELS
                     QVELSLEAASLASELSGSFRSLGAAEPLSFSFSASGSRPDSGSGAVAAAAAGEPAGPD
                     AGAAPGSATVVPSAEAAGAPAESAAAVAAAGGYRVQGRLKDWKYRGLQSRDWPFLLVS
                     RPEALSFSGGDGGELSFERRADGRFQAKAGAPLPFAFEASGRSAGGRLEAEVTGLGVD
                     LSPFLGL"
     gene            3201..3845
                     /gene="tmk"
                     /locus_tag="PLG14_09665"
     CDS             3201..3845
                     /gene="tmk"
                     /locus_tag="PLG14_09665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOX49437.1"
                     /translation="METNPVPTLKRFVVLEGVDGAGTTTQLRRLGEALGRAGVPHWTT
                     CEPTGMPTGRLVRQVLKGEVDARPETLARLYSADRHEHLYGPGGVLERLGRGEAVVSD
                     RYFFSSLAYQGMTCGPELPRLLNGDFPLPELLLYFDLDTEVSMARVETRAERDIFEHR
                     AFQERVRGAYEEVLAEYSGSPLRIARIDASLPVEEVFGAVAAAVGSVLGVDLTD"
     gene            3912..4517
                     /locus_tag="PLG14_09670"
     CDS             3912..4517
                     /locus_tag="PLG14_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49438.1"
                     /translation="MRRKQLLALALLLVLAAGGASAYPVYYKEQFFKLYHQHYIQYPD
                     DAIENIYWLEQAKKANFCNPLYALATIADERHWERYRYLFEMHLSLKLIEQHLALGSK
                     FDKRVAYFYNAPWKEQNLESLKTAESAYRAALAYWTEAKELAAKAMGMRFVQLEEVQF
                     WIDEAYRIDSGELDYADIIGDELGRLEKVRAAFEKMDASTY"
     gene            complement(4572..>4981)
                     /locus_tag="PLG14_09675"
     CDS             complement(4572..>4981)
                     /locus_tag="PLG14_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOX49439.1"
                     /translation="LPVRAEEIEGHAMHEEEYSVPEETVRAVARAKAEGRPVLAVGTT
                     SVRTLESAWREEGAEGPAGLRAGPGSTRIFIYPGYRFRAADRLFTNFHTPKSTLLMLV
                     SAFAGRERILAAYEEAVRERYRFFSYGDAMLIR"
BASE COUNT          791 a         1761 c         1824 g          605 t
ORIGIN      
        1 aggccgagga agggcgaaag gtcgacgccg agccccgtga cctcggcctc gagccttccg
       61 cccgcgctcc ttcccgaggc ctcgaaggcg aagggcaacg gcgccccggc cttggcctgg
      121 aagcgcccgt ccgcgcggcg ctcgaaggag agctcgccgc cgtcccctcc cgagaagctc
      181 agggcctcgg gccgcgagac gaggaggaaa ggccagtcgc ggctttgcag gccgcggtac
      241 ttccagtcct tgaggcggcc ctggacgcgg tagccgccgg ccgccgcgac ggcggctgcg
      301 gactccgcgg gagcgccggc ggcctcggca gagggcacga cagtggcgct ccccggagcc
      361 gccccggcgt cagggccggc aggctcgccg gccgccgcgg ccgcgaccgc gcctgagccg
      421 gaatcgggcc tcgagcccga ggccgagaag gagaagctca ggggctcggc ggcgcccagg
      481 ctccggaagg agcccgaaag ctccgaggcc agggaagcgg cctcgaggct gagctccacc
      541 tgggagagct ccattccccg ccaggccgcc ctggccttcc cgacgcggag caggccgtcg
      601 ccgaaggagc ccgagagtac gagctcgagc ggatcctcgg ccagggcgcc gtcccgcagc
      661 gaggcctcga aggagaggag gggcctctcg atgctgcccg agaccctggc ctttccgtcg
      721 acgcggcccg agagcgggcc cttcacgaag cgcgagacgg gggaagaggc gacctcgaag
      781 cgggcctcca ggctgtctcc gctgaggctc gccgcgaggt cgtagcgctc gccgcccttg
      841 ccggagagcc cccccttgaa cgacgccgag cgcgaggacc tggacaggtc gtagtcgagc
      901 cggcccgagc ccgagagcga ggactccgcg tcggagaggg agatggaggg gaagtcgccg
      961 cccccgggcc cgagcgtccc cgaaaggccg agcctcggca ggccggcccc cgcggagccg
     1021 gccagttcgg cgctcaagga ggagaggagg aggctccagt cggcgggccc cgcgtagcgg
     1081 ccgtcggcgt cgaggcccac gaccagggtc gcgcccttga gggggaccgg gaggccgtcg
     1141 gccctggcct cgaggtaggt ctcgcctccc atgccgcgga gcttgacctc gaggccgtag
     1201 tcgccgctca ggaagaggcc ctcgccggcg taggtgccct tcacggagta gggcacgtcc
     1261 tccagggaga gcttcgcctc gaagcccagg cgccccgcct cggcgaaatc cgcgctgccg
     1321 ctcgcctccg cgcggtagcc gccgtaggag acggaggcct tgcggaggga gacgcccttc
     1381 tcgctgcccg agacggagac gagggcgtag gccccaggcg aggacctgga gacgagggcg
     1441 aaatccgtcg tggaaaagga gaggcgctta aggtcgctga gcgcgaagac ctcgccgtcc
     1501 agccggaggt cggagaggag ggccgccgcc cccgacgaga cgagggccgc cgccaggggc
     1561 tcgaccgccg cgaggtcgaa ggattcgagg ccgagggaga gctcgaggac gggctcaggc
     1621 ccgccggaga ggctgccctc gcagcggaag cggggggagc cgcccgaggc ggcgaccgcc
     1681 tctcccgaga aggccggcgc ggcctcgtcg gggggaaggg ccaggatagg cggggcctcg
     1741 gcctcggggg ggcggaagga gaggccgaag tccaggcttc ccgcgccgcc cgagccgatc
     1801 gaggcgtcca gggcgaaatc gcggaaggcc acgccggctg cccggacgga gtccgagtag
     1861 gccgcgtaat ggccctggcc gcccaggacc cgcaagtccg cctcgagcgg cagccgtccc
     1921 tccgcgaggg aggcctccag cgcgagctcg ccgtccaggg aaaggccccg gaagccgagg
     1981 cttcccgagt aggaggcgcg tcccaggccg ccctcgaccc tggcgtaggc gatgtcggcg
     2041 ccctcgggcc cgcccttcgc ggagacggcg gcgcgagcca ggcttccgag actggagtcg
     2101 gccagctcgc ccgagagctc gagggagtag cgggtccgct tcgggtcgcc ggccagggcc
     2161 tcgaaggaga gggagcccga gaggggtatg cggagccagg gagagaggga ggagaggccc
     2221 ccgcgcaggg tcgccatcct cgcgggcgcg tagccctcca tgcgcagcga ggcggctaga
     2281 gcgcggccgc gcaggtcgta gcggacgaag gcgtcgaggg gggcctcgtc ctcgaccttc
     2341 ctgagctcgg cgacgccctg ggcgtaggag agctcgaagc gctgccgctc gagggcgaag
     2401 gagtccgagg aggccttgag ggacagcatg agtcgggccc gggagaaatc cctcgcgacg
     2461 aggccgtcga cgccgacggg aacctcgagc cgcccgatcc cgggggcgag ggccggatcg
     2521 acccgggccg agaggcggcc ggagaggagg agctgggcgt cgggcccgcc cttccctatc
     2581 ctgaaggaat cgatctcggc gcggagctcc cccggggcga agccggacaa cctgaggtcg
     2641 aggctcttcc ccgagaccgc gaaggagggc aggcccttgg agccgccgcc cccgcccccg
     2701 cctccgaagc gcgaggctag ggcggagagg cgctcgaggt cggcgcgccg caggtcggcc
     2761 ctgacgcgcg cgaagctgag ctcggatatc gcgagctggc ccttccccaa aaggagggcg
     2821 ccgaagtcgt agtaggcgta gaccctctcg gcggccagga ccgtcctccc ctctgcgtcc
     2881 ttgagctcga tccgcctgag ggagaaggcc ttgaggatcg aagggctcat ggactcgaag
     2941 gtgaggcgaa ggcccgtttc ccgctcgacc gaggcctgga ggccctggag ggcgccggag
     3001 agccggtccc ggaggtcccg gtccagggag ggcaggagga ggagggccgc gaggccgccg
     3061 gcgagtatga cgcccgataa cgcggcgggc gcgcgccgca atgttgccat tcccccatcc
     3121 tctcccagac acactacgat accacagccc cgcggctctc gtcttgccca gaaaggcttt
     3181 ccccggctat tctcgtcgcc atggaaacga atcccgtccc gacgctcaag cgcttcgtcg
     3241 tcctggaggg cgtggacggc gcgggcacca ccacccagct ccgccgcctg ggcgaggccc
     3301 tcgggcgggc gggcgtcccg cactggacga cctgcgagcc cacgggcatg cccaccggcc
     3361 gcctcgtgcg ccaggtcctc aagggcgagg tggacgcgcg cccggagacc ctcgccaggc
     3421 tctactccgc cgaccgccac gagcacctct acggcccggg cggcgtcctc gagcggctcg
     3481 gccgcggcga ggcggtagtc tccgaccgct acttcttctc cagcctcgcc taccagggca
     3541 tgacctgcgg cccggagctc cccaggctcc tcaacgggga cttccccctg cccgagctcc
     3601 tcctctactt cgacctcgac accgaggtct ccatggcccg cgtggagacg agggccgagc
     3661 gcgacatctt cgagcaccgc gccttccagg agagggtccg cggcgcctat gaggaggtcc
     3721 tcgcggagta ctcgggctcg cccctgcgga tagcgcggat cgacgcctcc ctgcccgtgg
     3781 aggaggtctt cggggccgtg gcggcggccg tcgggtcggt gcttggcgtc gatctcacgg
     3841 actagaaacc cccgagcggg ggaaaggttt cactttcccc gcggggcctg tccggccgat
     3901 actctagggg tatgcgccgc aagcagctcc tcgccctggc cctcctcctc gtcctggcgg
     3961 cagggggggc ctccgcctac cccgtctact ataaagagca gttcttcaag ctctaccacc
     4021 agcactacat ccagtacccc gacgacgcca tcgagaacat ctactggctc gagcaggcga
     4081 agaaggccaa cttctgcaat ccgctctacg ccctcgcgac gatagccgac gagcggcact
     4141 gggagcgcta ccgctacctc ttcgagatgc acctctccct caagctcatc gagcagcacc
     4201 tggccctggg ctccaagttc gacaagcgcg tggcctactt ctacaacgcg ccctggaagg
     4261 agcagaacct cgagagcctc aagacggccg agagtgccta ccgcgcggcc ctggcctact
     4321 ggacggaggc gaaggagctc gcggccaagg ccatgggcat gcgcttcgtc cagctcgagg
     4381 aggtccagtt ctggatcgac gaggcctaca ggatcgactc aggcgagctc gactacgccg
     4441 acatcatcgg ggacgagctt gggcgacttg aaaaggtgcg cgccgccttc gagaagatgg
     4501 acgcctcgac ctactgaatg agcgtcgcgg cgcccgcggc gcggacgccg cgccgcggaa
     4561 actgcgagac cctacctgat gagcatcgcg tcgccgtagc tgaagaagcg gtagcgctcc
     4621 cgcaccgcct cctcataggc ggcgaggatc ctctcccggc ccgcgaaggc ggagacgagc
     4681 atgaggaggg tcgacttggg cgtgtggaag ttcgtgaaga ggcggtccgc cgccctgaag
     4741 cggtagccgg ggtagatgaa gatcctcgtg ctgcccgggc ccgcgcggag gccggcgggg
     4801 ccctccgctc cctcctcgcg ccaggccgac tccagggtgc ggacgctggt cgtcccgacg
     4861 gcgaggacgg gccggccctc ggccttggcc cgggccacgg cgcggaccgt ctcctccggc
     4921 accgagtact cctcctcgtg catcgcgtgc ccctcgatct cctccgcccg gacggggagg
     4981 a
//
LOCUS       DAOOSJ010000201         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10565_length_4976_cov_7.275960, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000201 DAOOSJ010000000
VERSION     DAOOSJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10565_length_4976_cov_7.275960"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..551
                     /locus_tag="PLG14_09680"
     CDS             15..551
                     /locus_tag="PLG14_09680"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOX49440.1"
                     /translation="MALKATVYKADLHVADMDRNHYADHKVTLAREPSETDERMMVRL
                     LAFALDADERLELTADMSETEEPSLWNRGYDGRIRLWIEVGLPEEKRLRKAAARADSV
                     LLYLYHGRQARLWWEQNRAALEGLKNLRVVEVDPASVKALAARAEKTMDFQVTIQDGT
                     AWFADGEENVEVALERLK"
     gene            complement(659..1408)
                     /locus_tag="PLG14_09685"
     CDS             complement(659..1408)
                     /locus_tag="PLG14_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOX49441.1"
                     /translation="MDDEVEAALRRAAGVIREAEGLMAFTGAGISVESGIPPFRGPGG
                     VWGRYDPSLLEIGRFRADPAASWAAIREIFYASFGKAEPNAAHRVLASWEGEGLLGFL
                     VTQNIDGLHRKAGSRRLAEFHGAMDLLLCERCGERLAASADLLGSLPPRCPSCGGLLK
                     PDFVFFGEAIPREAYRASFASAEEAGACLVVGSTGTVYPAAELPLAVKRRGGVLIEID
                     PGTTEFSGLADVRIPLGASEALTRLDALLKS"
     gene            complement(1525..3153)
                     /locus_tag="PLG14_09690"
     CDS             complement(1525..3153)
                     /locus_tag="PLG14_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOX49442.1"
                     /translation="MKKLLKAALLGLAAALVLGCAGGPKAPAGGEVQIAVLHTNDTHG
                     HPLPFADHGVPEVSGLPAITTLVGRTRAEFENVLVLDAGDFNTGRPESNFFKAEPDIV
                     GFNLIGYDAVALGNHEFDNSREILEGQKALAEFPLLSANAKTKDGKLVADAAYVVKEF
                     QGVRVGVFGLTTRETATIGGPEVVRDLVFADEVETAKAMVAELRGKRKCQVVIALTHL
                     GVYENAAEGSRALAAKVPGIDLIVDGHSHTQMKEPLVENGVPIVQAWQWGLNVGRGVL
                     TVSGGKVAGFSWKPLTVNDKKAVKNADGSTRYEPLGPEFETNPIVNGKLIEYGYKVES
                     LLSEKIGSAAAAFPNAMSRKAESEIGGLVADSMKWRLRDQGVDFAIVGGGNIRSDLAA
                     GDITKKTAYTILPFDNSLVVLTMKGSELKELFDFLPTIPQGKGAFPQVSDGVSFTIDY
                     EKGATTDILVGGKPLDPAREYKVVTNSYMAAGGDGYAVFKKARAYDSSVYQRDALVDY
                     VKSLTAPLSPELKGRIKVVGQKLAWLFGGLLSRV"
     gene            complement(3507..>4976)
                     /locus_tag="PLG14_09695"
     CDS             complement(3507..>4976)
                     /locus_tag="PLG14_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOX49443.1"
                     /translation="AIGCAGAPKGPAPLASEVTITIAHTNDMHARAVSGKAELGYDRI
                     AGYINGLKAKNPNVLVLDAGDTLHGMPFANLERGASIVKLMNAVPYDYMTTGNHDYNY
                     GTPRLLELQKDMKFPILAANVYKDGKRLFKPYDIKVVGGVRVAVVGVASPETAYKADP
                     KGLDGATFTNPVTEVRTIANELAGSYDVFVLLSHVGLDRSSDPTSDMIANAVPEIDVI
                     IDGHSHTSLALAQSTNRSRTLIASTGSYDTGLGIVDIVVGKDRMVVSKTARTVGVSEP
                     GLASDPAVKSVIDGIVAAQAPMLAEKVGATAVELVGKREIVRTSESNLGRLIANSMLY
                     VTGADVSFIGGGGVRDTIPAGDITKKHIFTVLPFGNFIQTAKLKGSEFDAIIEHGVGK
                     LPAADGRFPHFAGLTYTLDPAAPAGERASNIMIGGKPVEPDKEYVFAATNFNYNGGDD
                     FKMLVGKKILNEYPTDAEIFMSYVKKLGTVTDANIEFKK"
BASE COUNT          728 a         1566 c         1877 g          805 t
ORIGIN      
        1 tcgggggtga tgccatggcg ttgaaagcga ccgtctacaa ggccgatctc catgtcgcgg
       61 acatggaccg gaaccactac gcggaccaca aggtcacgct ggcccgcgag ccctcggaga
      121 ccgacgagcg gatgatggtc cgcctcctcg ccttcgccct ggacgcggac gagcgcctcg
      181 agctcacggc cgacatgagc gagacggagg agccgagcct ctggaaccgc ggctacgacg
      241 gccggatccg gctctggatc gaggtgggcc tccccgagga gaagcgcctc cgcaaggccg
      301 cggcccgcgc cgactcggtc ctcctctacc tctaccacgg ccgccaggcc aggctctggt
      361 gggagcagaa cagggcggcc ctggagggcc tcaagaacct ccgcgtcgtc gaggtggatc
      421 ccgcctccgt gaaggccctg gccgcccgcg cggagaagac catggacttc caggtgacga
      481 tccaggacgg gaccgcctgg ttcgccgacg gggaggagaa tgtcgaggtg gccctggagc
      541 gcctcaagta gagcccggcg ccgccccgta ggccgctccg cgcggccggc ggccgcggcg
      601 tccattccgc gcggccggcg gcccgcttgt tcgggccgcg ccggcggccc gccggcgctc
      661 aggacttgag gagggcgtcg aggcgcgtga gggcctccga ggcgcccagg gggatccgga
      721 cgtcggcgag gccgctgaac tcggtcgttc ccgggtcgat ctcgatgagg acgccgccgc
      781 gccgcttcac cgcgaggggc agctcggccg cgggatagac ggtgcccgtg gagcccacga
      841 ccaggcaggc gcccgcctcc tccgcggagg cgaaggaggc ccggtaggcc tccctcggta
      901 tggcctcgcc gaagaagacg aagtcgggct tgaggaggcc gccgcaggag ggacagcgcg
      961 gaggcaggct cccgagcagg tccgccgaag ccgcgaggcg ttcgccgcat ctctcgcaga
     1021 ggaggaggtc catcgcgccg tggaactcgg cgagccgccg ggagcccgcc ttgcggtgga
     1081 gcccgtcgat gttctgggtg acgaggaagc cgaggaggcc ctccccctcc caggaggcca
     1141 ggacgcggtg ggcggcgttg ggctcggcct tcccgaagct cgcgtagaag atctcgcgga
     1201 tggccgccca ggaggcggcc ggatcggcgc ggaagcggcc tatctcgagg aggctcgggt
     1261 cgtagcggcc ccagaccccg cctgggcccc ggaagggagg gatgccgctc tccacggaga
     1321 tgcccgcccc cgtgaaggcc atgaggcctt ccgcctccct gatgacgccc gccgcccgcc
     1381 tgagggccgc ctcgacctcg tcgtccatgg aaaggagtat agccctcccg ccggcgcgca
     1441 aaaaggccgc ccccgggcgg ccggccttgc ggggcggcgt ccgggggcgg caggcgcgaa
     1501 tcggggagaa tccgctcagg atcgctagac gcgggagagg aggccgccga agagccaggc
     1561 gagcttctgg cccacgacct tgatgcgccc cttcagctcc ggggaaaggg gcgcggtgag
     1621 ggacttcacg tagtccacga gggcgtcacg ctggtagacg gagctgtcgt aggcgcgggc
     1681 cttcttgaac acggcgtagc cgtcgcctcc tgcggccatg taggagttgg tcacgacctt
     1741 gtactcccgg gcggggtcga ggggcttgcc gccgacgagg atgtcggtcg tcgcgccctt
     1801 ctcgtagtcg atcgtgaagg agacgccgtc ggagacctgg gggaaggcgc ccttgccctg
     1861 ggggatcgtc ggaaggaagt cgaagagctc cttgagctcc gagcccttca tcgtgaggac
     1921 gaccaggctg ttgtcgaagg gcaggatggt gtaggcggtc ttcttggtga tgtcgccggc
     1981 ggccaggtcg gagcggatgt tgccgccgcc cacgatcgcg aagtcgacgc cctggtcgcg
     2041 gagccgccac ttcatggagt cggcgacgag gccgccgatc tcggactcgg ccttgcggct
     2101 catggcgttg gggaaggccg cggcggcgga gccgatcttc tccgagagga ggctctccac
     2161 cttgtagccg tactcgatga gcttgccgtt gacgatgggg ttggtctcga actcggggcc
     2221 gaggggctcg tagcgcgtcg agccgtcggc gttcttcacg gccttcttgt cgttgaccgt
     2281 gaggggcttc caggagaagc ccgcgacctt gccgccggag accgtgagga cgccgcggcc
     2341 gacgttgagg ccccactgcc aggcctggac gatgggcacg ccgttctcga ccaggggctc
     2401 cttcatctgc gtgtggctgt ggccgtccac gatgaggtcg atgccgggca ccttcgcggc
     2461 cagggcgcgg gagccctccg cggcgttctc gtagacgccg aggtgggtca gggctatgac
     2521 gacctggcac ttccgcttgc cgcggagctc ggcgaccatg gccttcgcgg tctccacttc
     2581 gtcggcgaag accaggtccc tgacgacctc ggggccgccg atggtcgcgg tctcgcgggt
     2641 cgtgaggccg aagacgccga cgcggacgcc ctggaattcc ttcacgacat aggcggcgtc
     2701 ggcgacgagc ttcccgtcct tggtcttcgc gttggcggag aggaggggga actcggcgag
     2761 cgccttctgc ccctccagga tctcccggct gttgtcgaac tcgtggttgc ccagggcgac
     2821 ggcgtcgtag ccgatgaggt tgaagccgac gatgtccggc tcggccttga agaagttgga
     2881 ctcggggcgg cccgtgttga agtcgcccgc gtccaggacc aggacgttct cgaattcggc
     2941 cctggtccgg cccacgaggg tggtgatggc gggaaggccg gagacctccg gcacgccgtg
     3001 gtcggcgaag ggcaggggat gtccgtgcgt gtcgttcgtg tggaggacgg cgatctggac
     3061 ctcgccgcct gcgggcgcct tggggccgcc ggcgcagccc aggacgagcg cggccgcgag
     3121 gccgagcagg gccgccttga gtagtttttt cacgtgatac ctcctggcgt agcctttgcg
     3181 gacgggaagc ggatgcgagc cccctcgctc aaggatacca gaatactata tgcggtttct
     3241 aggaatctgt cacgggtatt attcccgtct attcgcccca aacccgggaa atgtcgtcta
     3301 tacacgagtg tcgcggagcg actggcgcgc gccacggcaa gcgcgccacc acctgcgtgc
     3361 cgcggcaagc gcgccgcgac cgcccgctcc ggacagggcg cctcgggacc tcggacaaac
     3421 cgcggataaa aagaagggcc gtcccccagg agccggcgcc aaggcgccga ctcgcagggg
     3481 acggccccgc aaggggcgat cgacgcctac ttcttgaact cgatgttcgc gtcggtgacg
     3541 gtcccgagct tcttcacgta gctcatgaag atctcggcgt cggtcgggta ctcgttgagg
     3601 atcttcttcc cgacgagcat cttgaagtcg tctccgccgt tgtagttgaa gttggtggcg
     3661 gcgaagacgt actccttgtc cggctcgacg ggcttgccgc cgatcatgat gttcgaggcg
     3721 cgctcgcccg cgggggcggc ggggtcgagg gtgtaggtga ggccggcgaa gtgggggaag
     3781 cggccgtcgg cggcggggag cttgccgacg ccgtgctcga tgatggcgtc gaactcggag
     3841 cccttgagct tcgcggtctg gatgaagttg ccgaagggga ggaccgtgaa gatgtgcttc
     3901 ttcgtgatgt cgccggcggg gatcgtgtcg cggacgccgc cgccgccgat gaaggagacg
     3961 tcggcgccgg tcacgtagag catggaattg gcgatgagcc tgccgaggtt ggactcggag
     4021 gtgcggacga tctcgcgctt gccgacgagc tcgacggcgg tcgcgccgac cttctcggcg
     4081 agcatcgggg cctgggcggc gacgatcccg tcgatgacgg acttcacggc ggggtcggag
     4141 gccaggccgg gctcgctcac gccgacggtg cgggcggtct tggagacgac catcctgtcc
     4201 ttgccgacga cgatgtccac gatgccgagg ccggtgtcgt aggagccggt gctcgcgatg
     4261 agggtgcggg agcggttggt cgactgggcc agggcgaggc tcgtgtggct gtggccgtcg
     4321 atgatcacgt cgatctcggg cacggcgttc gcgatcatgt cgctcgtcgg gtcgctcgag
     4381 cggtccaggc ccacgtggga gaggaggacg aagacgtcgt aggagccggc gagctcgttg
     4441 gcgatggtcc ggacctcggt gacggggttc gtgaaggtgg cgccgtcaag gcccttcggg
     4501 tcggccttgt aggcggtctc ggggctcgcc acgccgacga ccgcgacgcg gacgccgccc
     4561 acgaccttga tgtcgtaggg cttgaagagg cgcttcccgt ccttgtagac gttggccgcg
     4621 aggatgggga acttcatgtc cttctggagc tcgaggaggc ggggcgtgcc gtagttgtag
     4681 tcgtggttgc ccgtcgtcat gtagtcgtag ggcacggcgt tcatgagctt cacgatgctc
     4741 gcgccgcgct cgaggttggc gaagggcatg ccgtggaggg tgtcgcccgc gtccaggacg
     4801 aggacgttcg ggttcttggc cttgaggccg ttgatgtagc ccgcgatgcg gtcgtagccg
     4861 agctcggcct tgccggagac ggcccgggcg tgcatgtcgt tcgtgtgggc gatcgtgatc
     4921 gtgacctcgc tcgcgagggg agcggggccc ttgggagcgc cggcgcagcc gatcgc
//
LOCUS       DAOOSJ010000202         4972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10578_length_4972_cov_5.307708, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000202 DAOOSJ010000000
VERSION     DAOOSJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10578_length_4972_cov_5.307708"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1445
                     /gene="putP"
                     /locus_tag="PLG14_09700"
     CDS             6..1445
                     /gene="putP"
                     /locus_tag="PLG14_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HOX49444.1"
                     /translation="MILIGFFYMKRTKNASDFFLGGRSVGPVTAAISAEASDMSSWLL
                     MGLPGLAYLFGIQEALWTAVGLAIGTFVNWRFVAARLRRYTMRSGDAITIPEFLKNRF
                     RDRSNLLSVVSAFFILVFFGIYTASGFVACGKLFSSVFGMPYAAAVGLGAGVIVVYTL
                     LGGFLAVCATDVVQGMLMFVALLAAPVGAIIAVGGPAVAVERIAAVGPNFLNPFLSAS
                     GAPLPAMTLVSTLAWGLGYFGMPHILVRFMAVRSEREIPTSRRIAMAWVLVAMTAAVI
                     VGLAGRAYLAAPLDAAASETVFIKIAEGVFPAFVAGLFLCGILAATMSTADSQLLVTS
                     SAFSKDFYKAFLRKQAGDKEVVLVSRLTIVAVAVAGYFMALDPAGSIFRLVSYAWAGF
                     GATFGPIILLSLFWKRTTRNGALAGLLAGGGVTILWKQLSGGIFDLYEIVPGFAAGLA
                     AIVVVSLAGAPPPKEIVEEFEAVARMPVD"
     gene            1508..3031
                     /locus_tag="PLG14_09705"
     CDS             1508..3031
                     /locus_tag="PLG14_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49445.1"
                     /translation="MKSDSLGTAFAVAAPRYSDPSLQRLQERFDALAEEGAAPAELKR
                     FLSKVFVERNRAIRNQDFRKLAPASVAEDFLLPRRDLFAFLVENWISRIFLPSLREET
                     VESMLVFGLGRLFSSYNDTGVQRSTDADINVVVRDGLSAPRRAELREALTLLKRELFE
                     HFKINLEVDPSFTLLREKEIQARLQAADGATRLGNLLFYKSNSRSARIIKDEADLRAS
                     VFSRVARLPDALLFENFLGLANPKVSFMKLLTGESALPILADGACERIEASSVIGSRP
                     FSFRFHRLVPRRLLVAPPDWHFSMKYFVNRVYDYVGAMRNLGHGLGELGLGEVSPELG
                     VDPDYRFLRNAHRMMLYLQELEELSARSFNADCDYSYVSRARFMRFIEIDGDKFKRDF
                     VDMAVKGDLLLASEKAAFRALKRKIETKARDRFLSGRAAELKLLPPGLAYETVFKDKS
                     DFKIRLPYSWSDLGYFVFDRIAARMERIVLGKLVPPLSGLGMPEKDYRRYLELAKEA"
     assembly_gap    3064..3163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3164..4251
                     /locus_tag="PLG14_09710"
     CDS             <3164..4251
                     /locus_tag="PLG14_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX49446.1"
                     /translation="LAAFLALSLSACAKPPVKVGLPLVLTGLTSRIGIAGRNGALLAA
                     ERINAAGGIRGRRLELLPRDDFEDPDKALEVDQALAGEGAVALVGHMASRTGLKAVEF
                     ATRRRLPLISPTISSTDYSGKDDYFFRIIGSNALMGKTLAAYARKTGLSRAAAAYEST
                     NRSYTVPFFEGFKAEFERLGGVVLEPRSFATSASLDYGAMVEELLAEGPDSVFFVASP
                     FDTAEVCQALQTRGSPLPVLAAMWAMTDDLFAFGGRSVERLVGVMYLDPEDDSPAYRA
                     FREEYSRRYGEEPSFASAYGYDAVMVLAKAMAAARRLDGPSIKEALLGIGRFDGLQGQ
                     IVLDRYGDCEKEPFVFRVRDGRFQRAP"
     gene            4425..>4972
                     /locus_tag="PLG14_09715"
     CDS             4425..>4972
                     /locus_tag="PLG14_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOX49447.1"
                     /translation="MEVSRYLDAPARALFSLDTFLAAGPGDAMVQAFMDSELGARPEL
                     VSVLLLDEEGRVLLSSPEDRRALGQDYSGQPSFKEAKTAKGVILSPPYVSPADGSVTI
                     AAYGRAGRGFGAVTLKLEALSDFLSSLRLSPLDRIGIADGAGRFAAHTDMAFVREQRY
                     WPGRPAEGKPVVEDGRSWIAATA"
BASE COUNT          681 a         1806 c         1522 g          863 t
ORIGIN      
        1 tggcgatgat cctcatcggc ttcttctaca tgaagcggac gaagaacgcc tcggacttct
       61 tcctgggggg gcgcagcgtc gggcccgtga ccgcggccat cagcgccgag gcctcagaca
      121 tgtcgagctg gctcctcatg ggccttcccg gcctggccta tctcttcggc atccaggagg
      181 cgctctggac ggccgtcggc ctggccatcg gcaccttcgt caactggaga ttcgtcgccg
      241 ccaggctgcg ccgctacacg atgcgctcgg gcgacgccat caccatcccg gaattcctga
      301 agaaccgctt ccgcgacagg tcgaacctcc tctccgtcgt ctcggccttc ttcatcctcg
      361 tcttcttcgg catctacacc gcctccggct tcgtcgcctg cgggaagctc ttctcctcgg
      421 tcttcggcat gccctacgcg gccgccgtgg gcctgggcgc cggcgtcatc gtggtctaca
      481 ccctccttgg aggcttcctg gcggtctgcg ccaccgacgt cgtccagggc atgctcatgt
      541 tcgtcgccct tctcgcggcg cccgtgggcg cgatcatcgc cgtcggcggc cccgccgtcg
      601 ccgtcgagcg catcgccgcc gtcggcccga atttcctcaa tcccttcctc tccgcctccg
      661 gggctcccct cccggccatg accctcgtct ccaccctggc ctggggcctg ggctacttcg
      721 gcatgcccca catcctggtg cgcttcatgg ccgtgcgctc cgagagggag atcccgacct
      781 cgaggcggat agcgatggcc tgggtcctcg tcgccatgac ggccgccgtg atcgtgggcc
      841 tggccggacg ggcctacctc gctgccccgt tggacgcggc ggcctcggag acggtcttca
      901 tcaagatcgc cgagggggtc ttcccggcct tcgtggccgg cctcttcctc tgcggcatcc
      961 tggccgcgac gatgagcacg gccgactccc agcttctcgt gacctcctcc gccttctcca
     1021 aggatttcta caaggccttc ctgcgcaagc aagcgggcga caaggaggtg gtgctcgtga
     1081 gccgcctgac catcgtcgcc gtcgccgtgg cgggctattt catggccctc gatcccgcgg
     1141 gctccatctt ccgcctggtg agctacgcct gggccggctt cggggcaacc ttcggcccca
     1201 tcatcctcct ttccctcttc tggaagcgga cgacccggaa cggcgccctg gccggcctcc
     1261 tcgcgggggg cggcgtgacc atcctctgga agcagctctc aggtggcatc tttgacctgt
     1321 atgagatcgt gccgggcttc gcggccgggc tcgccgccat cgtcgtggtg agcctcgcgg
     1381 gcgcgcctcc ccccaaggaa atagtcgagg agttcgaggc cgtggctcgt atgccggtag
     1441 attgactaat ctttggataa tggagaaaat aagccgctag cccttctccc gcgaggtctt
     1501 tctcccgatg aagtccgact ctctaggcac ggccttcgcc gtagcggcac cccgatactc
     1561 cgacccctcc ctgcaaaggc tgcaggagcg ctttgacgcg ctggccgagg aaggggcggc
     1621 gcccgcggag ctcaagcgct tcctctccaa ggtcttcgtc gagcggaacc gcgccatccg
     1681 caaccaggac ttccggaagc tcgcccccgc cagcgtcgcg gaggactttc tcctcccgcg
     1741 cagggacctc ttcgccttcc tggtggagaa ctggatctcc cgcatcttcc tcccctccct
     1801 cagggaggag acggtcgagt cgatgctcgt cttcggcctg ggaaggctct tctcctccta
     1861 caacgacacg ggagtccagc gctccaccga cgcggacatc aacgtcgtcg tccgcgacgg
     1921 cctttccgcc ccccggcggg cggagctccg cgaggccctc accctcctca agcgcgagct
     1981 cttcgagcac ttcaagatca acctggaggt cgatccctcc ttcaccctcc tccgcgagaa
     2041 ggagatccag gcgcggctcc aggccgcgga cggcgcgacg aggctcggaa acctcctctt
     2101 ctacaagagc aacagccgct cggccaggat catcaaggac gaggcggacc tgcgcgcctc
     2161 ggtcttctcc cgcgtcgccc gcctcccgga cgccctcctc ttcgagaatt tcctggggct
     2221 cgccaatccc aaggtctcct tcatgaagct cctcacgggg gagtccgccc tgcccatcct
     2281 ggccgacggc gcctgcgaga ggatcgaggc ctcttccgtc atcggctcga ggcccttctc
     2341 cttccgcttc caccgcctcg tgccgcgcag gctcctcgtg gcgcctcccg actggcactt
     2401 ctccatgaag tacttcgtga accgcgtcta cgactacgtc ggcgcgatgc ggaacctggg
     2461 ccacggcctc ggggaactgg gcctgggcga ggtgtctccc gagctcggcg tcgaccccga
     2521 ctaccgcttc ctccgcaacg cgcaccggat gatgctctac ctccaggagc tcgaggagct
     2581 gtcggcgcgg agcttcaacg ccgactgcga ctacagctac gtctcccgcg cccgcttcat
     2641 gcgcttcatc gagatagacg gcgacaagtt caagcgggac ttcgtcgaca tggccgtgaa
     2701 gggagacctc ctcctcgcga gcgagaaggc ggccttccgc gccctgaagc gcaagatcga
     2761 gaccaaggcc agggaccgct tcctctcggg acgggcggcc gagctcaagc tcctgccgcc
     2821 gggcctggcc tacgagaccg tcttcaagga caagtccgac ttcaagatcc gcctgcccta
     2881 ctcctggtcc gacctgggct acttcgtctt cgacaggatc gccgctcgca tggagaggat
     2941 cgtgctcggg aaactcgtgc cgccgctttc ggggctcggc atgccggaga aggactaccg
     3001 ccgttacctg gagctggcca aggaggctta ggcccgggag gccaagccgc gaatcgtggt
     3061 cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcttggc ggccttcctc
     3181 gccctttccc tctcggcctg cgcgaaaccg ccggtcaagg tgggccttcc cctcgtcctc
     3241 acgggcctga cctccaggat cggcatagcc gggcgcaacg gcgccctcct cgccgccgag
     3301 cggatcaacg cggcgggcgg gatacggggc cggcgcctcg agctcctgcc gcgcgacgac
     3361 ttcgaggatc cggacaaggc cctggaggtg gaccaggccc tggccgggga gggcgccgtc
     3421 gccctggtgg gccacatggc gagccgcacg ggcctcaagg ccgtggaatt cgcgacccgg
     3481 cgccgactgc ccctcatatc tccgaccatc tcctccacgg actactcggg caaggacgac
     3541 tacttcttca ggatcatcgg ctcgaacgcc ctcatgggga agaccttggc cgcctacgcg
     3601 aggaagaccg ggctctcgcg ggccgcggcg gcctacgaga gcacgaaccg ctcctacacg
     3661 gtgccctttt tcgagggctt caaggccgag ttcgagcggc tgggcggcgt cgtcctggag
     3721 ccgcggagct tcgccacctc ggcgagcctc gactacggcg cgatggtgga ggagctcctc
     3781 gccgaggggc ccgactcggt cttcttcgtc gcgagcccct tcgacacggc agaggtctgc
     3841 caggcgctgc agacgcgcgg cagccccctc cccgtcctgg ccgccatgtg ggcgatgacc
     3901 gacgacctct tcgccttcgg cggccgcagc gtcgagcgcc tcgtcggcgt gatgtacctc
     3961 gatcccgagg acgactctcc cgcctatcgc gccttccgcg aggagtactc ccggcgctac
     4021 ggcgaggagc cgagcttcgc ctcggcctac ggctacgacg ccgtgatggt gctcgccaag
     4081 gccatggccg ccgcgcgccg cctggacggc ccttcgataa aggaggcgtt gctcgggatc
     4141 gggcgcttcg acggcctcca gggccagatc gtcctggacc gctacggcga ctgcgagaag
     4201 gagcccttcg tcttccgggt gagggacggc cgcttccagc gcgcgccctg aaggagcgga
     4261 acctgcgcac caccttcagc ctgaggcgct tcttcacggc gagcgccttc gccctggccc
     4321 tcctgcccgt cctcctcgtc gtcgtcctct cggagcgcat cctcttcgcg tcgctggagc
     4381 gggacatcgc cgcctccgtc ggctccatct ctaggtcggt cgccctggag gtctcccgtt
     4441 atctcgacgc gccggcgcgc gccctcttca gcctggacac cttcctcgcc gccgggcccg
     4501 gagacgccat ggtccaggcc ttcatggaca gcgagctggg cgcgaggccg gagctcgtct
     4561 ccgtcctcct cctggacgag gaggggcggg tcctcctctc gagcccggag gaccggcgcg
     4621 cgctgggcca ggactactcg ggccagccct ccttcaagga ggcgaagacg gcgaaaggcg
     4681 tcatcctctc ccctccctac gtctcgcccg ccgacggctc ggtgacgatc gcggcctacg
     4741 gcagggccgg aaggggcttc ggggccgtca cgctgaagct cgaggccctg tcggacttcc
     4801 tctcctcctt gcggctctcc cccctcgacc gcatcgggat cgccgacggg gcgggccgct
     4861 tcgccgcgca cacggacatg gcctttgtgc gggagcagcg ctactggccg ggaagaccgg
     4921 cggagggaaa gcccgtcgtc gaggacggca ggagctggat cgcggcgacg gc
//
LOCUS       DAOOSJ010000203         4851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10948_length_4851_cov_8.197665, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000203 DAOOSJ010000000
VERSION     DAOOSJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10948_length_4851_cov_8.197665"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            666..1220
                     /locus_tag="PLG14_09720"
     CDS             666..1220
                     /locus_tag="PLG14_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX49448.1"
                     /translation="MDCLSAIKGRRSVRKYRSDPVPEETFQALMESVRWAPSWANTQC
                     WEIIAVDDARTKAELALTLGGPNPAASALAEAPLVLVLCGRKGVSGLRRGEPATDKGD
                     WLMFDVGLATQTLCLAARDLGLGTVIVGLFDHRKVAEALGVPPEVEVVAMTPLGYPAE
                     EEGAPRRKDVSEFVFFGRHPGKRP"
     gene            1318..2388
                     /locus_tag="PLG14_09725"
     CDS             1318..2388
                     /locus_tag="PLG14_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOX49449.1"
                     /translation="MRPEDLAESLKLILARSPAFPGEALAVDLIANPKAGGFTRPAYA
                     KKRKAELEALEARAAALPLRGAPASLRLHLTERSGHAADLARGVVEEARRDPPGLRRL
                     VLTAGGDGTSLETASALVELPPAERSRFALLRLPFGTGNDGSEGRDLEACLGRLLGPL
                     SFAPRRAVRVSPNPAGGKAPLYSFNIASVGLDAYVCDMTNRLKTAFPGDSYKFWVDVS
                     SVLYDRIWPPAPLRLRGLDSGGRETAAFERKCLLFAFGASGNRQYGANKPILPDVDNA
                     CAIFQMPLLRKLALKDRMASGGHRGLGEDVVKLFSAERVEIEYGRGLLLQRDGEVSEL
                     SPADFPLVMELTEPLYNVLERA"
     gene            2412..3296
                     /locus_tag="PLG14_09730"
     CDS             2412..3296
                     /locus_tag="PLG14_09730"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX49450.1"
                     /translation="MARIESMLSLPHGAKVIAAALPIRRDPPGAKEAVLLLHGFTGHP
                     GELALQAEALAAAGYAVSVPRLPGHGSGRADFLATDEKDWLRRALDAALELRAAYGTV
                     HLLGHSMGGLLAALVASALQGGESGSQAAAGRGASASGPTASAQGGAAAPRLVLLAPA
                     FLLSKAGVAAAPFVAPFAPVLRRGRPVPDSEALDPVRSYLHGEYRSDDLVREAAALRR
                     IQLAARRRLPALRSRLLVVVGSEDASVPPRVAAYIEAKARSALGVEVRLLPGAGHVFP
                     FDARGPEAAALVSAWMRG"
     gene            complement(3495..4682)
                     /locus_tag="PLG14_09735"
     CDS             complement(3495..4682)
                     /locus_tag="PLG14_09735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49451.1"
                     /translation="MSDLMPSFAASMREVDRAEWDALASPSGSPLLSWGFLSLLEESG
                     SVSPDTGWTPAHLLLRRGGRLVAAAPFYLRGDSWGDFVFDFELAEAAESLRAPYYPKL
                     VGMVPATPAPAWKVLVAEGEDEAALSALVLAAAEETARASGLAGLQLNWVDPAFEPLV
                     RGSGRFLEWSHQGFLWRDGDAAGRNYGDFEGYLAAFSKNMRRNVLREREGLAAAGIES
                     RVLGAREAAATPGLLARMADFYEATNDKFGPWAARFLSRDFFLRLPEFLPEGWILSAG
                     YERDAPLGEPLGLAFLLEGEERLYGRYWGAARTEPGLHFELCYYRPIEYALERGLSSF
                     DPGMGSEHKARRGFRSVLSPSFHLAFDRRVAALLSRYLPLASEEAAAEAKALDRELPF
                     KEE"
BASE COUNT          641 a         1848 c         1674 g          688 t
ORIGIN      
        1 cggggggtca aaactgaatg tcgcccgggg gtcaattctg gatgtcgctt gacaggactc
       61 actccttccc gccgcgatcc tctgtcgtgg gggggcttcg gaaatactcc tacctacctc
      121 gaaggtagct ctgcctacct taaaggtagc tctacttacc tagaaggtag ctgtgcctac
      181 ctccgaggta cccataccta cctccgagct tcgtttttct atctccgagc tccgagtgcc
      241 tatctccgag ctccgacttc ctatctcgga gatccgagtg cctgtctccg agctccgatt
      301 ttgtatctcc gagctccggc gtcgaggctc cgagctccgg cgccgggtcc ccaagggccg
      361 attcacccga atgggggaaa ggcgaggccg ggcgcgatcc ctatgatgtc gccatcgcgt
      421 ggacacgccg atcgtcgatc gcgcgcgttc cttccggagg cggcctagca gggaccgcgt
      481 ccggaagggg gaggacgcct cctacccagg atgctgatgc gaacgccccg cctccccggg
      541 gcttttttta ttgagccgac gggccggccc gcgaggccga accctcgagg ccgcgcccct
      601 tggccggcgc gcgggccgcg accgcctcga gcaggcccgg atcgccggcg aggaaggagc
      661 gtgacatgga ctgcctgtcc gcgatcaagg gaagacggag cgttcggaag tacaggtccg
      721 accccgtgcc ggaggagacc ttccaggccc tcatggagtc ggtgcggtgg gcgccttcct
      781 gggccaacac gcagtgctgg gagatcatcg cggtcgacga tgcccggacc aaggccgaac
      841 tggccctcac ccttggcggc ccgaacccgg ccgcctcagc gctggccgag gcgccgctcg
      901 tcctcgtcct ctgcggcagg aagggcgtgt ccggactgag gcggggagag cccgcgaccg
      961 acaagggcga ctggctcatg ttcgacgtcg gcctcgcaac ccagaccctc tgcctcgccg
     1021 cgcgggacct cggcctgggc acggtgatcg tcgggctctt cgatcaccgg aaggtcgcag
     1081 aggccctggg cgtccccccc gaggtcgagg tcgtcgcgat gacgcccctc ggctatcccg
     1141 cggaggagga aggcgccccg agacggaaag acgtctccga attcgtcttc ttcggccgcc
     1201 acccggggaa acgtccctag cgccgggagt ggccgtgcgg cgccctcccg cgcgacgccc
     1261 tcccgcgcga aggctttgcg gtggacaagg ctccggagcg gcgctagact ctcgcccatg
     1321 cgtccagaag acctcgcgga atccctcaag ctgatcctcg cgcgctcgcc ggccttcccc
     1381 ggcgaggccc tcgccgtgga cctcatcgcg aacccgaagg ccggcggctt cacccgcccc
     1441 gcctacgcga agaagcgcaa ggccgagctc gaggcgctcg aagcccgcgc cgcggccctt
     1501 ccgctccgcg gcgcgcccgc gagcctgagg ctccacttga ccgagcgctc gggccacgcg
     1561 gccgacctgg cccgcggcgt cgtcgaggag gcgaggcgcg acccgcccgg cctccgccgc
     1621 ctcgtcctca ccgcgggcgg cgacggcacc tccctggaga cggcgagcgc cctcgtcgag
     1681 ctgccgccgg cggagcggag ccgcttcgcc ctcctccgcc tgcccttcgg gacgggcaac
     1741 gacggctcgg agggccgcga cctcgaggcc tgcctgggcc gcctcctcgg gcccctatcc
     1801 ttcgcgcccc gccgcgcggt gcgggtgtcg cccaacccgg cgggaggcaa ggcgcccctc
     1861 tactccttca acatcgcgag cgtgggcctg gacgcctacg tctgcgacat gacgaaccgc
     1921 ctcaagaccg cctttcccgg cgactcctac aagttctggg tggacgtctc ctccgtcctc
     1981 tacgacagga tctggccgcc cgcccccctg cgcctccgcg gcctggactc gggcggccgc
     2041 gagaccgcgg ccttcgagcg gaagtgcctc ctcttcgcct tcggcgcctc ggggaaccgg
     2101 cagtacggcg cgaacaagcc catcctcccc gacgtggaca acgcctgcgc catcttccag
     2161 atgccgctcc tcaggaagct cgcgctcaag gacaggatgg cctccggcgg ccatcgcggc
     2221 ctgggcgagg acgtcgtcaa gctcttctcc gccgagcgcg tggagatcga gtacggccgg
     2281 ggcctcctcc tccagcgcga cggcgaggtc tccgagctct cgcccgccga cttccccctc
     2341 gtgatggaac tcacggaacc cctctacaac gtgctggaac gggcctaggc gagggcccga
     2401 aggaggacgc catggcgcgg atcgagtcga tgctgtccct gccccacggg gcgaaggtca
     2461 tcgcggccgc gctgccgatc cgccgcgatc ctcccggcgc gaaggaggcc gtcctcctcc
     2521 tccacggctt cacgggccac ccgggcgagc tcgccctcca ggccgaggct ctcgcggccg
     2581 cgggttacgc ggtctctgtt ccgcgccttc ccggccacgg gagcggccgc gccgacttcc
     2641 tcgcgaccga cgagaaggac tggctccgcc gggccctcga cgccgccctc gagctccgcg
     2701 ccgcctacgg gaccgtccac ctcctcggcc actcgatggg agggcttctc gcggccctcg
     2761 tggcctccgc cctccagggc ggcgagtccg gatcgcaggc ggcggccggg cgcggcgcct
     2821 cggcctcggg gccgaccgcc tccgcgcagg gcggcgccgc ggctccgcgc ctcgtcctcc
     2881 tcgcgccggc cttcctcctg tccaaggccg gcgtcgcggc cgcgcccttc gtcgcgccct
     2941 tcgcgcccgt cctgcggcgc ggcaggcccg tgccggactc cgaggccctg gatcccgtcc
     3001 gctcctatct ccacggcgag taccgctcgg acgacctggt ccgcgaggcc gcggccctca
     3061 ggcgcatcca gctcgcggcg cggcgccgcc ttccggccct gcgctcgcgc ctcctcgtcg
     3121 tggtcgggag cgaggacgcg agcgtcccgc cccgggtcgc cgcctacatc gaggccaagg
     3181 cccgctccgc cctcggcgtc gaggtccgac tccttccggg cgccggccat gtcttcccct
     3241 tcgacgcgcg gggccccgag gcggccgccc tggtctcggc ctggatgcgg ggctagcctc
     3301 cgcgcttcgc cgccgggccg cccctgcgta gcgccgcgcc ggcgcctcgt ccgcgggatc
     3361 gccctgcgcg ccttgccgtc acgccgcgcc cgcgccgcct cgccgcggcg ccgcgcgggc
     3421 ccgcgcgacc gggcctggcg gccgcctcgc cgcttccgcg ccgccagccg cgcccgcgcc
     3481 gcctcgggcg gcgctcactc ctccttgaag ggcagctctc tgtccagagc tttcgcctcg
     3541 gccgccgcct cctcgctcgc gagggggagg tagcgcgaaa ggagggcggc gacgcggcgg
     3601 tcgaaggcga ggtggaagga gggggagagg acggagcgga agccccgcct cgccttgtgc
     3661 tcgctcccca tgcccggatc gaagctcgac agcccgcgct ccagggcgta ctcgatcggc
     3721 ctgtagtagc agagctcgaa gtggaggccc ggctcggtcc tggccgcgcc ccagtagcgg
     3781 ccgtagagcc tctcctcccc ctcgaggagg aaggcgaggc cgaggggctc gcctaagggc
     3841 gcgtcccgct cgtagcccgc ggagaggatc catccctcgg gaaggaactc aggcaggcgg
     3901 aggaagaagt cgcgggaaag gaagcgcgcg gcccaggggc cgaacttgtc gttcgtcgcc
     3961 tcgtagaagt ccgccatccg cgcaaggagg cccggcgtcg ccgccgcctc cctcgcgccc
     4021 aggacgcggc tctcgatccc cgccgccgcg aggccctcgc gctcgcgcag gacattgcgc
     4081 cgcatgttct tggagaaggc ggcgagatag ccttcgaagt ccccgtagtt ccgtcccgcc
     4141 gcgtcgccgt cccgccagag gaagccctgg tggctccatt ccaggaagcg gcccgagccg
     4201 cggaccaggg gctcgaaggc ggggtcgacc cagttgagct ggaggccggc caggcccgag
     4261 gcccgcgcgg tctcctccgc cgcggccagg acgagggcgg agagcgccgc ctcgtcctcg
     4321 ccctcggcga cgaggacctt ccaggccggg gcgggcgtcg cggggaccat gccgacgagc
     4381 ttcggatagt agggcgcgcg cagggactcg gccgcctcag cgagctcgaa gtcgaagacg
     4441 aagtcgcccc aggagtcgcc gcggaggtag aagggcgcgg cggcgacgag ccgccccccg
     4501 cgcctaagga ggaggtgggc cggcgtccag cccgtgtcgg gcgagaccga gcccgactcc
     4561 tcgaggaggg agaggaagcc ccaggagagg aggggcgagc ccgagggaga ggccagggcg
     4621 tcccactccg cgcggtcgac ctcgcgcatc gaggccgcga aggaaggcat gaggtcggac
     4681 atgccttaag gataacgctt cgagcgggcc ttgggcgcct gtcctgaggg cttttcaggg
     4741 ctcttcgcgc gggctttttt cggccgcctc gcgcctggcc aggaagccgt cgatctcgtc
     4801 cttgccgaag gcgtaggtcg tgccgcagtt gtggcagtgc gcctccacgg g
//
LOCUS       DAOOSJ010000204         4849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10957_length_4849_cov_4.818732, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000204 DAOOSJ010000000
VERSION     DAOOSJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_4849_cov_4.818732"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLG14_09740"
     CDS             <1..777
                     /locus_tag="PLG14_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49452.1"
                     /translation="EKAAAAVKSQGRAVLRIVGPPGALVSGFSDERLEIPAEGEIELS
                     LKAPATYLWKARASGFESAEGIVSLFEESASFDLDLKRVRAWTLEVGLKNAAFPDFWA
                     SYRLLGDRLFVKAGFEQYLFGLALTRERVDYDPPYFLSIRMIEPGLGVGWLFGKAGGE
                     TRPYAALTLAARLAFPKDAGFFLDPVAPLQVQPAAGIEWKPHESWGFFLELGSSLYLL
                     GDGILMAGSVGEDRGGTLYAYGETWFLEFPSFRFGARMHL"
     gene            774..1874
                     /locus_tag="PLG14_09745"
     CDS             774..1874
                     /locus_tag="PLG14_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49453.1"
                     /translation="MTRPASRRASRLTKLAIALAASAALASCWVPVYDPAASLAMRLE
                     RKLEKVTELGPFPMNEWEARKGYFLPHRTLAPVDGFWIARPEERIRVRYLAEGLTYWG
                     SWESGYNALGGGFSAVALTDAAASGLSTSVFKRGLVLVYETGGYTGIYLDATNNFIPD
                     PGILVGGINPRIVGAYASPSGPNTLELAALQYSTQDHELWGGATTIAASSAFAPSTAN
                     ISQAKPNVYYGLDLKPGAFFAWNTSDYFLSGRLNSDDSLVTIRWNAGLSDSSPTLLSG
                     IDRQLTDILYDDRLLARGELTTIVYSNGGTRLFEIPTGSLRFVHELYDMATTTWYCYF
                     SRSTYVYGKDDSGQLYVEVFRIPSASLSDLAQ"
     gene            complement(1984..4440)
                     /locus_tag="PLG14_09750"
     CDS             complement(1984..4440)
                     /locus_tag="PLG14_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49454.1"
                     /translation="MSMTTEGTTGLLKRASALRSSQAGEDSAELDRRSGITPEDRKEI
                     QARIEELTKGARLTAEPGLFKVKAKSSGLLFPVLVNLAAALLVAGGLFGLSYLFRERE
                     AAVAQSDVAVSSAEGKLIRELQRESASKLDEKDKEIADIQLRLASLDKERGALAATIE
                     ERVRARELELRAALDAELRRERERLLAEGLSEAVIQERLKKFEAEKTAAFRRELAEFQ
                     AKAEAERQAAEANYQKLRDEYQKSIAGLAEERKRIQDESRRREEELRASLEASMAART
                     QSLQAKSAEAEAGLAAAKAELARVQDEKAKATAAEDRVVGLYGSVRQALRDRRFDEAA
                     LQAAALRSYLDDPGVANLPGLQARRSADIFAAETLAAYAKSELDRAGSDAARLLRQAE
                     LLQAALDSAAAGERALKDGDGALAERKYREALEKSPEILKAHEYFLARSGAAVAEERA
                     RLDTRLLQAEEAYKAGDYPAAAARYQEALAYLPIEESARLKIVSRLAEGGAFEADKAR
                     RAADTKAAREPLAQARRDLAASRFGPALAGYVGLLASFPAADQAPEARRGAETARLGL
                     AKAAEDAAKAAEGDSAALRAEAERLARELESETRRLSEETRLARAEGASALESLAAAK
                     DARISELERLLETARAETAAARAAAPGAAAPGATGGQAPKGQAAPAPGSEAALAGLQR
                     DIDTLKAENERLAAASDRYDAVLSSYKDYLGAEDAVLASGGANALVEGRAKLDAFLSG
                     PAASAALPGLRERIARYERAFQEAGQREVLFNAMDIVENALRQRDEASRDRYFKDLAA
                     RYSGDATMLEFITGLRKSIK"
     gene            complement(4496..>4849)
                     /locus_tag="PLG14_09755"
     CDS             complement(4496..>4849)
                     /locus_tag="PLG14_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX49455.1"
                     /translation="RSEEELDESIEAALNASAELAAGSPLVVLTHAPPHGTEADRRHG
                     SHVGSRAFRSILEEVAPLLWVSGHIHESRSVSREEATLLVNPGPLHDGFYAVAELFRG
                     PGGFRAEAELARLGS"
BASE COUNT          629 a         1688 c         1718 g          814 t
ORIGIN      
        1 gaaaaggcgg ccgccgccgt caagtcccaa gggcgcgccg tgctgaggat cgtcggcccg
       61 ccaggcgccc tcgtctcggg cttctcggac gagcgtctcg agattccggc cgagggggag
      121 atcgagctct ccctcaaggc tcccgcgacc tatctttgga aggctcgggc ctcaggcttc
      181 gagtccgccg aaggcatcgt ctccctcttc gaggagagcg cctcattcga cctcgacctc
      241 aagcgcgtca gggcctggac cctcgaagtc ggattgaaga acgcggcctt cccggatttc
      301 tgggcgtcct accgccttct tggcgacagg ctcttcgtga aggcgggctt cgagcagtac
      361 ctcttcggct tggcgctgac gcgcgagcgc gtcgactatg acccgcccta cttcctgtct
      421 atccgcatga tcgagcccgg actgggcgtg ggctggctct tcggaaaggc cggaggggag
      481 acccgcccct acgccgcgct gacgctcgcg gcgcggctcg ctttccccaa ggacgcgggc
      541 ttcttcctcg atccagtcgc cccgctccag gtgcaacccg cagcaggaat agagtggaag
      601 ccccacgaaa gctgggggtt tttccttgaa ctcggatcca gtctttatct cctcggggac
      661 ggcatcctga tggccggttc cgtaggcgag gaccggggcg gcacgctcta cgcctacggc
      721 gagacctggt tcctggaatt cccgagtttc cgcttcggcg cgaggatgca cctatgaccc
      781 gtccggcctc tcggcgcgcg agccgcctga ccaagctcgc catcgcgctg gccgcctcgg
      841 ccgcgctggc ctcctgctgg gtgccggtct acgatccagc cgcttcgctc gccatgcgcc
      901 tggagcgaaa gctcgagaaa gtaaccgagc tcggcccctt cccgatgaac gaatgggagg
      961 ccaggaaggg ctatttcctc ccccatagga cgctggcgcc ggtggacggc ttctggatcg
     1021 cccggccgga ggagaggatc cgcgtccgct acctcgccga gggactgacg tattggggaa
     1081 gctgggaatc gggatacaac gccttgggcg gcggcttctc ggccgtcgcg ctgaccgacg
     1141 ccgcggcttc ggggctctcg acctccgttt tcaagcgcgg ccttgtgctt gtctatgaga
     1201 cgggcggcta cacgggaatc tacctcgacg ctactaacaa cttcattccc gatccgggca
     1261 tcctcgtcgg gggtatcaac cctcgcatcg tcggcgccta cgcgtcgccg tcggggccaa
     1321 acacgttgga gctggccgcc ctccagtaca gcacacaaga ccacgaacta tggggcggcg
     1381 ccacgacgat cgccgcctcc tcggccttcg ccccgtcgac cgccaacatc agccaggcga
     1441 agccgaacgt ctactatggc ctcgatctca agccgggcgc cttcttcgcc tggaacacct
     1501 cggactattt tctctccgga aggctgaact ctgacgacag tctcgtgact atccgctgga
     1561 acgcgggcct ctccgactcg agcccgaccc tgctctccgg catcgaccgg caactgaccg
     1621 acatcctcta cgacgaccgc cttctcgccc ggggcgagct cactacgatc gtctactcaa
     1681 acggcgggac tcggcttttc gagattccaa cgggctcctt gcgctttgtc catgagcttt
     1741 acgacatggc gaccactacc tggtactgct acttcagccg ctccacgtat gtctacggaa
     1801 aagacgactc gggccagctc tatgtagaag tcttccgcat cccgagcgca agcctctcgg
     1861 acttggctca atagtaggcg ccactccatg ttccgcagga aacaagcgag ggcggccctt
     1921 cggccgccct ctcctatttc cgtcctcaag cgcgccaagt tcggcgtgct tcggcgccgt
     1981 cccctacttg atgctcttcc tgagccccgt gatgaactcc agcatcgtcg cgtcgcccga
     2041 gtagcgggcc gcgaggtcct tgaagtagcg gtcgcgggag gcctcgtcgc gctggcgcag
     2101 ggcgttctcc acgatgtcca tcgcgttgaa gaggacctcg cgctggccgg cctcctgaaa
     2161 ggcgcgctcg tagcgcgcga tgcgctcgcg gaggcccggc agggcggcgc tcgcggccgg
     2221 ccccgagagg aaggcgtcga gcttggcgcg gccctcgacc agggcgttcg cgccgcctga
     2281 ggcgaggacg gcgtcctcgg ccccgaggta gtccttgtag ctggagagaa ccgcgtcgta
     2341 gcggtcggag gcggcggcca ggcgctcgtt ctccgccttc agggtgtcga tgtcgcgctg
     2401 aaggccggcg agggccgcct cggagccggg cgccggggcg gcttggccct tgggggcctg
     2461 gccgcccgtg gctccgggcg ccgccgcgcc gggcgcggcc gcgcgggctg cggcggtctc
     2521 ggcgcgggcc gtctcgagga ggcgctcaag ttcggagatg cgggcgtcct tcgcggccgc
     2581 gaggctttca agggcggacg cgccttcggc gcgggccagg cgggtctcct cggagaggcg
     2641 cctcgtctcg gactcgagct cgcgggccag gcgctcggcc tcggccctga gggcggcgga
     2701 gtctccctcg gccgccttgg ccgcgtcctc cgcggccttg gcaaggccga ggcgggccgt
     2761 ctcggcgccg cgcctggcct cgggcgcctg gtcggccgcc gggaaggagg cgaggaggcc
     2821 gacgtagcct gcgagggccg ggccgaagcg ggaggccgcg aggtcgcgcc tggcctgggc
     2881 caggggctcg cgggcggcct tggtgtcggc ggcgcggcgg gccttgtcgg cctcgaaggc
     2941 gccgccctcc gccaggcggg agacgatctt gagcctcgcg gactcctcga tcgggaggta
     3001 ggccagggcc tcctggtagc gggcggcggc ggcggggtag tcgcccgcct tgtaggcctc
     3061 ctccgcctga agaaggcggg tgtccaggcg ggcccgctcc tccgcgaccg cggcgccgct
     3121 ccgggccagg aagtattcgt gggccttgag gatctcgggc gacttctcca gggcctcgcg
     3181 gtacttccgc tcggccaggg cgccgtcgcc gtccttgagg gcgcgctcgc cggcggcggc
     3241 ggagtccagg gcggcctgga ggagctcggc ctggcggagg agccgcgccg cgtcggagcc
     3301 cgcgcggtcg agctccgact tcgcgtaggc cgcgagggtc tccgccgcga agatgtcggc
     3361 ggaccgcctg gcctggaggc cgggcaggtt ggcgacgccg ggatcgtcga ggtaggagcg
     3421 cagggcggcg gcctggaggg ccgcctcgtc gaagcggcgg tcgcgcaggg cctggcggac
     3481 gctcccgtac aggccgacga cgcggtcctc ggcggcggtc gccttggcct tctcgtcctg
     3541 gacgcgggcc agctcggcct tcgctgcggc gagcccggcc tcggcctcgg cgctcttggc
     3601 ctggaggctt tgggtcctgg cggccatgga ggcctcgagg ctcgctcgga gctcctcctc
     3661 gcggcgcctc gactcgtcct ggatgcgctt ccgctcctcg gccaggcccg cgatgctctt
     3721 ctggtactcg tccctgagct tctggtagtt cgcctcggcg gcctggcgct cggcctcggc
     3781 cttggcctgg aactcggcga gctcgcggcg gaaggcggcc gtcttctccg cctcgaactt
     3841 cttgaggcgc tcctggatga cggcctcgga gaggccctcc gcgaggaggc gctcgcgttc
     3901 gcgcctgagc tcggcgtcca gggcggcgcg gagctcgagc tccctcgccc tgacccgctc
     3961 ctcgatggtc gccgcgaggg ctccgcgctc cttgtccagg ctggccaggc gcagctggat
     4021 gtcggcgatc tccttgtcct tctcgtcgag cttggaggcg gattcgcgct ggagctcgcg
     4081 gatgagcttg ccctccgcgg aggagacggc gacgtcgctc tgcgcgaccg cggcctcccg
     4141 ctcccggaag aggtacgaga ggccgaagag gcctccggcc acgaggaggg ccgcggccag
     4201 gttgacgagg acggggaaga ggaggcccga gcttttcgcc ttcaccttga agagcccggg
     4261 ctcggcggtg agccgggcgc ccttggtgag ctcctcgatc ctcgcctgga tctccttcct
     4321 gtcctccggc gtgatgcccg agcggcggtc gagctccgcc gagtcctctc cggcctggga
     4381 ggacctgagc gcgctcgctc tcttgaggag gcctgtcgtt ccttcagtcg tcatgctcat
     4441 accgcttcac ctaaggatag tcgcatatcg ccgccgccgc aacgctttcc gggcgctagg
     4501 agccgaggcg ggcgagctcc gcctccgccc ggaagccgcc ggggccgcgg aagagttcgg
     4561 ccaccgcgta gaacccgtcg tggagggggc ccggattgac gaggagggtc gcctcctccc
     4621 gggaaacgga gcgggactcg tggatgtggc cggagaccca gaggaggggc gcgacctcct
     4681 ccaggatgga gcggaaggcc cgggagccga cgtggctgcc gtggcgcctg tccgcctccg
     4741 tcccgtgggg cggcgcgtgc gtgaggacga cgaggggcga gccggcggcg agctcggcgg
     4801 aggcgttgag ggccgcctcg atggactcgt ccagctcctc ctccgagcg
//
LOCUS       DAOOSJ010000205         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_10969_length_4847_cov_4.922788, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000205 DAOOSJ010000000
VERSION     DAOOSJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_4847_cov_4.922788"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLG14_09760"
     CDS             complement(<1..262)
                     /locus_tag="PLG14_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOX49456.1"
                     /translation="MKRIAILYGGKSGEHEVSRTSAASVVRNLDPKRFEVLLVGIAKE
                     GAWYLQEEGLAAKVRAGLSAIPVEKGPRVFLAPSGGPSGGLVA"
     gene            complement(259..1809)
                     /locus_tag="PLG14_09765"
     CDS             complement(259..1809)
                     /locus_tag="PLG14_09765"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOX49457.1"
                     /translation="MASKLSEIVRGLELLERSGPQELDIEGLAYDSREVKPGYLFFAL
                     PGLHEDGRRFIGQAVAAGAVAVVHEGVLEGKAPGVAYLRVASSRFAMSPVADAFHRSP
                     SRSMAVLGVTGTEGKSTTVYLIYQLLKLAGRKAGFFSTVMSDTGRGEEPNPEHQTTPE
                     ATAVQRMLAAMRDSGCEYAVVEASSHGLSPRLNRLGDVAFDAGVFMNVTHEHLEFHGS
                     WERYRDDKANLFRALDRFPHDKIFGGVPRAVPAFGVTCADDPSAAYFAAATGKPTRTF
                     SSKGAPRADFRAQGIRPDAAGSSFAIEAEGRRAEARIELPGAFNVDNALAALIAVSGI
                     TGLDWETLVPLLPRLKAVRGRMTAVDRGQPFEVIVDYAHTPSSFKAVFPPLRGRAKGR
                     MLCVFGSGGERDVEKRPQQGRIAADWFDVVILADEDPRGEDPTELLEAIAAGCPELRR
                     GEELFVIPDRPQAIRKAFSLAGPGDMVLLLGKGHENSIIYKDGPRPYDEIAEAERALA
                     ELGYKEKA"
     gene            complement(1951..2487)
                     /locus_tag="PLG14_09770"
     CDS             complement(1951..2487)
                     /locus_tag="PLG14_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49458.1"
                     /translation="MKRFALALVVLALVAAPAFSQLRLDIGIDVPRGVGAAFQGSAEV
                     SQETVDFFNNYFIPFPEAGLYYQYDLGMFKFGLGARAYTFIVETILWPNAFAEVHLGP
                     VVVEAQVGGGLFFMVGLANQFEAGKVFLPDLSAWFKIGKKQNLRLGAGVLGLYLPELT
                     TEGFGFVYYLGGKVALNL"
     gene            2611..2970
                     /gene="queD"
                     /locus_tag="PLG14_09775"
     CDS             2611..2970
                     /gene="queD"
                     /locus_tag="PLG14_09775"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOX49459.1"
                     /translation="MYRVRVEASFAAAHFLSHYHGKCERLHGHNYRVRAFAKGPALDE
                     GGMLLDFGALKGALREVLEGLDHSLLNDEPAFKGDPSAERIAELIFRRMAALLPDAPL
                     EAVEVFETETSMARYEP"
     gene            2960..>4847
                     /locus_tag="PLG14_09780"
     CDS             2960..>4847
                     /locus_tag="PLG14_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOX49460.1"
                     /translation="MSRELPPEGAPEGAPEGGAARDYASTRLIAYLGNKRALLPFLAS
                     VFDELEARAPLSSFLDPFAGSGAVSRLARSRGLRVAASDAEDYSYAVNACWLGVPAEA
                     LPGLFPEEGGLEAVLGRLNRLHPARGDEDIPHEPYIARHYAPASTEGADWRRERLFYT
                     RENAVFLDRAREAVERLRPAPRPPEALRAGAPAAATGQADEAGVAAAAAGKALSRAEM
                     ERALLLGPLVYEAATHANTSGVFKACHKGFGGHGRDALGRIMAPMRLEPPLLWKGPGA
                     EVARSDAAAFCAGRSADLCYLDPPYNQHQYGSNYHLLNALARWEKPPVSEERLPDGSL
                     RRKAGIDPGWARSRSAFCSRTGAAEAFRELLAAVDARFIVLSYNDSGIVPPEELYDLL
                     SDRAAVSIRSSGYVSYRGGRQSAERRLASRELLFVAERSGRAGRAAGVAAGAASAAAF
                     GEARSGTSCAASGAAPEAAAGTARGAAALPRDLELAALKAEIRLSSALASALSVERLR
                     LLPLRAEGAPGLYREADGLVYAWEGGELLLPTHRDLLFAPSAPAFAASSLPASERESL
                     AGLLEPLLARDNAEAAASAALLLEEGASEARLQRFALSRLRKLAHPKYRDSYEALFAR
                     LEAAA"
BASE COUNT          683 a         1736 c         1755 g          673 t
ORIGIN      
        1 cggcgacgag gccgcccgag gggccgcccg agggcgcgag gaagacccgc gggcccttct
       61 ccacggggat cgcggagagg ccggcgcgga ccttcgcggc aaggccctcc tcctggaggt
      121 accaggcccc ttccttggcg atgccgacga ggagcacctc gaagcgcttc gggtccaggt
      181 tcctgacgac gctggccgcc gaggtccggg agacctcgtg ctcgcccgac ttgccgccgt
      241 agagtatcgc tatccgcttc atgccttctc cttgtagccg agctcggcga gcgcgcgctc
      301 ggcctcggcg atctcgtcgt agggccgggg cccgtccttg tagatgatcg aattctcgtg
      361 gcccttcccg aggaggagga ccatgtcgcc cggcccggcc agggagaagg ccttgcggat
      421 ggcctgcggc ctgtcgggga tgacgaagag ctcctcgccg cggcgtagct cgggacagcc
      481 cgcggctatc gcctcgagga gctcggtcgg gtcctcgccg cggggatcct cgtcggcgag
      541 gatgacgacg tcgaaccagt cggcggcgat gcggccctgc tggggccgct tctccacgtc
      601 gcgctcgccg cccgagccga agacgcagag catccggccc ttcgcccggc cgcgcagcgg
      661 cgggaagacc gccttgaagg aggaaggcgt gtgggcgtag tcgacgatga cctcgaaggg
      721 ctggccgcgg tcgacggcgg tcatgcggcc ccgcacggcc ttgagccgcg ggaggagggg
      781 gacgagggtc tcccagtcca ggcccgtgat gcccgacacc gcgatgaggg cggccagcgc
      841 gttgtcgacg ttgaaggccc cggggagctc gatcctggcc tcggcgcgcc gaccctccgc
      901 ctcgatcgcg aaggaggagc cggcggcgtc ggggcggatg ccctgggcgc ggaagtcggc
      961 gcgcggcgcg cccttggagc tgaaggtcct cgtgggcttg ccggtcgccg cggcgaagta
     1021 ggcggcggag gggtcgtcgg cgcaggtgac gccgaaggcg gggacggccc gagggacgcc
     1081 gccaaagatt ttgtcgtggg ggaagcggtc cagggcgcgg aagaggttgg ccttgtcgtc
     1141 ccggtaccgc tcccagcttc cgtggaactc caggtgctcg tgggtgacgt tcatgaagac
     1201 gcccgcgtcg aaggccacgt cgcccaggcg gttgagccgg ggggagaggc cgtgggagga
     1261 ggcctcgacg acggcgtatt cgcagcccga atcgcgcatc gccgctagca tgcgctggac
     1321 ggccgtggcc tcgggcgtcg tctggtgctc gggattgggc tcctcgcccc tccccgtgtc
     1381 ggacatcacg gtggagaaga agccggcctt cctgcccgcg agcttgagga gctggtagat
     1441 caggtagacc gtcgtgctct tgccctcggt cccggtgacg cccaggacgg ccatggagcg
     1501 cgagggcgag cggtggaagg cgtcggcgac gggggacatc gcgaagcggc tcgaggcgac
     1561 gcggaggtag gcgacgcccg gggccttgcc ctcgagcacg ccctcgtgca ccacggcgac
     1621 ggccccggcc gcgacggcct ggcctatgaa gcggcggccg tcctcgtgga ggcccgggag
     1681 ggcgaagaag aggtagccgg gcttcacctc gcgggagtcg taggcgaggc cctcgatgtc
     1741 gagttcctgg gggccggagc gctcgaggag ctcgaggccg cggacgattt cggacagctt
     1801 ggatgccatg gcgctaccat agcaccgggc ctccgggcct agcaaggacc gccccttgcg
     1861 acggcttccg gagggaagcc ttcgcaaggg gcggggatca ggaccggccg cggcggccgg
     1921 tcggagggaa gcgcgggacc caggcgcccg ctagaggttg agggccacct tgccgcccag
     1981 gtagtacacg aagccgaagc cctccgtggt cagttcgggc aggtagaggc cgaggacgcc
     2041 ggcgccgagc ctcaagttct gcttcttgcc gatcttgaac caggcggaga ggtcggggag
     2101 gaagaccttg cccgcctcga actggttcgc gaggccgacc atgaagaaga ggccgccgcc
     2161 gacctgggcc tccacgacga cggggccgag gtggacctcg gcgaaggcgt tgggccagag
     2221 gatggtctcg acgatgaagg tgtaggccct ggctcccagg ccgaacttga acatgccgag
     2281 gtcgtactgg tagtagagcc cggcctccgg gaaggggatg aagtagttgt tgaagaagtc
     2341 caccgtctcc tgggagacct cggcgctgcc ctggaaggcg gccccgacgc ccctcgggac
     2401 gtcgatgccg atgtccagcc tgagctggga gaaggcggga gccgcgacca gggccagcac
     2461 cacgagcgcc agcgcgaaac gtttcatgca gaacctcctg tttcgggctc cgcgcgccgg
     2521 aaccccaatt atcactatag accatcgcgc ttcgcttttc acgcgccctc gcatcaggta
     2581 gaatgcccgc gagaaggggg acacaagcgc atgtacagag tccgcgtcga ggcgagcttc
     2641 gccgccgccc acttcctgag ccactaccat ggcaagtgcg agcgccttca cggccacaac
     2701 taccgagtcc gggccttcgc caagggtccc gccctcgacg aggggggcat gctcctcgat
     2761 ttcggtgcgc tcaagggcgc cctccgcgag gtcctcgagg gcctcgacca cagcctcctc
     2821 aacgacgaac cggccttcaa gggcgacccg agcgccgagc gcatcgccga actgatcttc
     2881 aggaggatgg ccgccctcct ccccgatgcg cccctggagg cggtggaggt cttcgagacg
     2941 gagacgagca tggctcgcta tgagccgtga gctgccgccc gagggcgcgc ccgagggcgc
     3001 gcccgagggc ggcgcggcca gggactacgc ctccacccgc ctcatagcct acctcggcaa
     3061 caagcgcgcc ctccttccct tcctggcctc ggtcttcgac gagctcgagg cgcgcgcgcc
     3121 cctctcctcc ttcctggatc ccttcgcggg ctccggggcg gtgagccgcc tggcgcggag
     3181 ccgcggcctg cgggtcgcgg cctccgacgc ggaagactac tcctacgccg tgaacgcctg
     3241 ctggctcggc gtccccgcgg aggccctgcc cggcctgttc cccgaggagg gcggcctcga
     3301 ggccgtcctt gggcgcctca accgcctcca tcccgcgcgc ggggacgagg acattcccca
     3361 cgagccctac atcgcccggc actacgcccc ggcgagcacg gaaggggccg actggcgccg
     3421 cgagcggctc ttctacacga gggagaacgc ggtcttcctg gacagggccc gcgaggccgt
     3481 cgaacggctc aggcccgcgc cgcggccgcc ggaagcgctc cgggccggcg ctccggcggc
     3541 ggcaaccggc caagccgacg aggccggcgt cgccgcggcg gcggccggca aggccctctc
     3601 gcgggcagag atggagcggg ccctccttct cggacccctg gtctacgagg cggccaccca
     3661 cgccaacacc tcgggcgtct tcaaggcctg ccacaagggc ttcgggggcc atggccgcga
     3721 cgccctcggc cgcatcatgg ccccgatgcg cctggagccg cccctcctct ggaaggggcc
     3781 gggggccgag gtcgcgcgct ccgacgcggc ggccttctgc gcggggcgga gcgccgacct
     3841 ctgctacctc gacccgccct acaaccagca tcagtacggc tccaactacc acctcctcaa
     3901 cgccctggcg cgctgggaaa agccccccgt ctccgaggag cggctgccgg acgggagcct
     3961 caggcgcaag gccggcatcg accccggctg ggcgaggagc cgctccgcct tctgctcgag
     4021 gacgggcgcg gcggaggctt tccgcgagct cctggccgcg gtggacgcgc gcttcatcgt
     4081 cctctcctac aacgactcgg gcatcgtccc gcccgaggag ctctacgacc tcctttccga
     4141 ccgggccgcg gtctccatcc gctcctcggg ctacgtgagc taccgcgggg ggcggcagag
     4201 cgcggagagg cgcctcgcct cgagggagct cctcttcgtc gccgagcgct cgggccgggc
     4261 gggccgggcg gccggcgtcg ccgcaggagc cgcttccgcg gcggccttcg gggaggcgcg
     4321 atccgggacg tcatgcgcgg cctccggggc cgcgcccgag gcggccgccg ggacggcgcg
     4381 aggcgccgcc gcccttccgc gcgacctcga gctcgcggcc ctgaaggcgg agatcaggct
     4441 ctcctcggcc ctggcctcgg ccctctcggt cgagcgcctg aggctccttc ccctcagggc
     4501 cgagggcgct cccggcctct accgcgaggc cgacggactt gtctacgcct gggaaggcgg
     4561 agagctcctt ctcccgaccc accgggacct tctcttcgcg ccttcggccc cggccttcgc
     4621 ggcctcctcc ctgcccgcct ccgagcggga gagcctggca ggcctccttg agcctctcct
     4681 tgcccgcgac aacgccgagg ccgcggcctc ggccgccctc cttctcgagg aaggagcctc
     4741 cgaggcccgg ctccagcgct tcgccctctc gcgcctgcgg aagctcgcgc acccgaagta
     4801 ccgcgactcc tacgaggcgc tctttgcgag gctcgaggcg gcggccg
//
LOCUS       DAOOSJ010000206         4832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11022_length_4832_cov_4.466611, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000206 DAOOSJ010000000
VERSION     DAOOSJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11022_length_4832_cov_4.466611"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLG14_09785"
     CDS             complement(<1..786)
                     /locus_tag="PLG14_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOX49461.1"
                     /translation="MSIRHAILGFLSWQPSTGYELKKRFAEATPFHWSGNNNQVYGAL
                     IELHREGLVQVEVVQQEKRPARKVYAITERGREALEGWLREGSEPPETRADFHARLAW
                     SASLPEAEFERLASAYEERLEGEILYRRERERRGEDRPARDARERLIWASLALNRIDA
                     LEAGERQRNLAVHRHQAHAVDLDVAGTEPAHVILQHFQLGRVVGRLVVQPKNVLLHLR
                     FLLRYPCFRWRISNCQRLNTCFISSEFIFRITVPSIISSVKTVT"
     gene            917..1768
                     /gene="tsf"
                     /locus_tag="PLG14_09790"
     CDS             917..1768
                     /gene="tsf"
                     /locus_tag="PLG14_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOX49462.1"
                     /translation="MKIDVAALKELRARTEAGMLDCRKALEASLGDLEAAASLLAEWG
                     LAGAARREEREAREGRVFAACGPEGAALLELSCETDFAARSAPFLAAGGKAAEAARDR
                     RLSEPDGGLAALVAGLGPALREKVALRRLAWLGRGEGELVEAYLHGEGRIGVLVKCGA
                     RPAGALLDGEVRGLVRDLALQVAAFGPRFLEAASLPEGELEAMAADIRAEVEADPLLG
                     AKPLAVRERAAAGRLAKRLAADSLLDRGFVRDEGRSVREAIAEVEARRGCELRVAGFA
                     RFEVGAE"
     gene            1972..2553
                     /locus_tag="PLG14_09795"
     CDS             1972..2553
                     /locus_tag="PLG14_09795"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOX49463.1"
                     /translation="MPETKVLINLSNRHIHVSQEDLELLFGKGHALTKTKDLMQPGQF
                     ACEETVTIVGPKGKFENVRILGPVRKETQCEVLASDSFKLGIKSIPCRESGKLDGSAA
                     FEIVGPAGTVKKAQGLIVAKRHIHFDPAAAERFGVKDTELVDLVIPGERGATFHNVVC
                     RVHPTYALECHLDFDEGNGVGIGNGSIGEIVKK"
     gene            2550..3614
                     /locus_tag="PLG14_09800"
     CDS             2550..3614
                     /locus_tag="PLG14_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOX49464.1"
                     /translation="MSRVVLKAQDLDGYLGEGDKVSLARMDALYREAMLSFSILSSAH
                     SEEEKRKEEARLIDRYNEMGSLMQEICASEPRLQVYSFVTPSESHGEASRLIAKLRDV
                     GTGRQEFVYYIQRAYELLFNMTFGGAQAQNKNYLIVKTPVTAPVQNYAVHKIPNLDEA
                     LDDTVMCVMLRGALLPSMIMSKEIQEFSSRGYVTPFALFKIKRDDSKSETTMEYILDL
                     DRSYFKLEALDGKDLVFADPMNATGGSLVTVIKYLQDKGVRPRSVKFVNVISALKGAL
                     RIVRAIDNATVHTLWMDPVLNDRAYIMPGLGDAGDRINGKDEDEAPRDMLRLIADYGS
                     NITSLYRSQLRTIEETVLRR"
     gene            3652..4557
                     /locus_tag="PLG14_09805"
     CDS             3652..4557
                     /locus_tag="PLG14_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49465.1"
                     /translation="MSRLRVASALCLAALLCAAALASCATAPASAAALEWYEVGNAWF
                     DAGKWDRAGEAYSRALALDPKLGAASYNMLRALAEAGDHEGALRAADLLLATDPGNVR
                     VLAAKAYVLSRSGEPEAALEVYSELLALDPFSPDAVYNAAWLKAEAGDEAGAAADLKR
                     LVEARPEDSGALALYAKLLYGLGPKAEALAAHERLKAQGKADAAALERLGGLYAEGRD
                     FAKAMEAFAAATAAEPKRASSWFALARLRLVEAEDGKGGLEALAKALEAGFADKAAAA
                     ALLGERNLVEREAVAKALREKGLAP"
BASE COUNT          741 a         1733 c         1692 g          666 t
ORIGIN      
        1 cgtgactgtc ttaactgatg agatgatcga cggcacggtg attcggaaga tgaactcgga
       61 cgagatgaag catgtgttca aacgctggca gttcgagatt cgccagcgga agcacgggta
      121 acgcaagagg aaacgaagat gaagaagtac gttcttcggc tgaacgacga gacggccaac
      181 aacccggcca agctggaagt gttgaaggat gacgtgggcc ggctcggtgc ccgcaacatc
      241 gaggtccacg gcatgggcct ggtgacggtg gacagcgagg ttccgctgtc gttcgccggc
      301 ctccagggcg tcgatccggt tcagggcgag ggaggcccag atgaggcgct cgcgggcgtc
      361 ccgcgccggg cggtcctcgc cgcggcgctc gcgctcgcgc ctgtacagga tctccccctc
      421 gagccgctcc tcgtaggccg aggcgaggcg ctcgaactcg gcttccggca gggaggccga
      481 ccaggccagg cgcgcgtgga aatccgcgcg ggtctcggga ggctccgaac cctcgcgcag
      541 ccagccttcc agggcctccc ggccccgctc cgtgatggcg tagaccttcc tcgccggccg
      601 cttctcctgc tggaccacct cgacctggac caggccctcc cgatggagct cgatgagggc
      661 gccgtagacc tggttgttgt tgcccgacca atggaagggc gtggcttcgg cgaagcgctt
      721 cttgagctcg tagcccgtgg agggctgcca ggacaggaag ccgaggatgg cgtggcggat
      781 ggacatgggg gctcctcgac ctagctatag cctaccttgc tctggcttga caatagtata
      841 aaactaacat aggttatatg taacctatgt gattggccct gggcggctcg cgccggctcg
      901 agggaggagg aatgccatga agatcgatgt cgccgccctg aaggagctcc gcgcgaggac
      961 ggaggccggc atgctcgact gccggaaggc cctcgaggcc tccttgggcg acctcgaggc
     1021 ggccgcctcc ctcctggctg aatggggcct cgcgggggcg gcccggcgcg aggagcggga
     1081 ggcccgggaa gggagggtct tcgcggcctg cggccccgag ggagcggccc tcctcgagct
     1141 ctcctgcgag acggacttcg ccgcccgctc cgctcccttc ctggccgcgg gcggaaaggc
     1201 cgccgaggcc gcgcgggaca ggcgcctctc ggagcccgac ggcgggctcg cggccctggt
     1261 cgcgggcctg ggcccggcgc tcagggagaa ggtcgcgctc cgtcgcctcg cctggctcgg
     1321 ccggggggag ggggagctcg tcgaggccta cctccacggc gaggggcgga tcggcgtcct
     1381 cgtgaagtgc ggggccaggc cggcaggcgc gttgctggac ggcgaggtgc gaggccttgt
     1441 ccgcgacctg gccctgcagg tcgcggcctt cgggccgcgc ttcctggagg cggcgagcct
     1501 tcccgagggc gagcttgagg cgatggccgc ggacatccgc gccgaggtcg aggccgatcc
     1561 cctgctcggg gcgaagcccc tggcggtccg ggagcgggca gccgcgggaa ggctcgcgaa
     1621 gcgcctggcc gcggacagcc tcctggaccg gggcttcgtg cgcgacgagg ggcggagcgt
     1681 ccgcgaggcg atcgcggagg tcgaggcgag gcggggctgc gagcttcgcg tcgcgggctt
     1741 cgcccgcttc gaggtcgggg ccgagtaggg cggcccgggg ccactcgggc ggcggcctcc
     1801 tcggggtcgg ctccgcgcgc cgggccagcg cgccaagccc gcgcgccggg gccgccggaa
     1861 ggccagcgcc ccttgacccg ccgggcgggg ggcgtctact ctatcatgac cgcctagcag
     1921 atcccggccg ccgccttccg gcggccccca tatcctcgaa ggagtcacgc catgcccgag
     1981 accaaggtgc tcatcaacct ctcgaaccgc catatccacg tttcccagga ggacctcgag
     2041 ctcctcttcg gcaagggcca cgccctcacg aagacgaagg acctcatgca gcccggccag
     2101 ttcgcctgcg aggagaccgt gacgatcgtg ggcccgaagg gcaagttcga gaacgtgcgc
     2161 atcctcgggc cggtccgcaa ggagacgcag tgcgaggtcc tggcctccga ctccttcaag
     2221 ctcggcatca agagcatccc ctgccgcgag tcgggcaagc tcgacggctc cgccgccttc
     2281 gagatcgtcg gccccgccgg gacggtcaag aaggcccagg gcctcatcgt cgcgaagcgc
     2341 cacatccact tcgaccccgc cgcggcggag cgcttcggcg tcaaggacac ggagctcgtc
     2401 gacctcgtca tccccggcga gcgcggcgcc accttccaca acgtcgtctg ccgcgtccac
     2461 ccgacctacg ccctcgagtg ccacctcgac ttcgacgagg gcaacggcgt cgggatcggg
     2521 aacggctcca tcggggagat cgtcaagaaa tgagccgcgt cgtcctcaag gcgcaggacc
     2581 tggacggcta cctcggcgag ggcgacaagg tctccctggc caggatggac gcgctctacc
     2641 gcgaggcgat gctgtccttc tccatcctct cctccgccca ctccgaggag gagaagcgga
     2701 aggaggaggc ccgcctcatc gaccgctaca acgagatggg ctccctcatg caggagatct
     2761 gcgcgtccga gccccgcctc caggtctact ccttcgtgac cccctcggag agccacggcg
     2821 aggcgagccg gctcatcgcg aagctccgcg acgtgggcac gggccggcag gagttcgtct
     2881 actacatcca gcgcgcctac gagctcctct tcaacatgac cttcggcggc gcccaggccc
     2941 agaacaagaa ctacctcatc gtgaagacgc ccgtcacggc ccccgtgcag aactacgcgg
     3001 tccacaagat cccgaacctg gacgaggccc tggacgacac ggtgatgtgc gtgatgctcc
     3061 gcggcgccct cctcccctcc atgatcatgt cgaaggagat ccaggagttc tcgtccaggg
     3121 gctacgtgac gcccttcgcc ctcttcaaga tcaagcgcga cgactccaag agcgagacga
     3181 cgatggagta catcctggac ctggaccgct cctacttcaa gctcgaggcg ctggacggca
     3241 aggacctcgt gttcgccgac ccgatgaacg cgacgggcgg aagcctggtc acggtcatca
     3301 agtacctcca ggacaagggc gtcaggcccc gctccgtcaa gttcgtcaac gtcatctcgg
     3361 ccctgaaagg agcgctccgc atcgtccgcg ccatcgacaa cgcgacggtc cacacgctct
     3421 ggatggaccc cgtcctcaac gaccgggcct acatcatgcc gggcctgggc gacgcgggcg
     3481 accgcatcaa cggcaaggac gaggacgagg cgccccgcga catgctccgc ctcatcgcgg
     3541 actacggctc gaacatcacg agcctctacc gctcccagct caggaccatc gaggagacgg
     3601 tgctcaggcg ctagcctggg ccgggcgggg gcggggaggg aggcttccag gatgagcagg
     3661 ctccgagtcg cctctgccct ctgcctcgcc gccctcctct gcgcggccgc cctcgcgtcc
     3721 tgcgcgacgg cgccggcctc ggccgccgcc ctggagtggt acgaggtcgg caacgcctgg
     3781 ttcgacgcgg ggaagtggga ccgcgcgggg gaggcctact ccagggccct ggccctggac
     3841 ccgaagctcg gcgcggcctc ctacaacatg ctgcgcgcct tggccgaggc cggggaccac
     3901 gagggcgcgt tgcgcgcggc cgacctcctc ctcgccacgg atcccgggaa cgtccgggta
     3961 ctcgcggcca aggcctacgt cctctcgcga agcggcgagc ccgaggccgc cctcgaggtc
     4021 tactcggagc tcctcgccct cgatcccttt tcccccgacg ccgtctacaa cgccgcctgg
     4081 ctcaaggccg aggcgggcga cgaggcgggg gcggccgcgg acctgaagcg cctcgtcgag
     4141 gcgcggcccg aggactcggg cgccctcgcc ctctacgcga aactgctcta cgggctcggt
     4201 cccaaggcgg aggcgctggc cgcccacgag cggctcaagg cccagggcaa ggccgacgcg
     4261 gccgccctcg agcgcctggg cggcctctac gccgagggcc gcgacttcgc caaggccatg
     4321 gaggctttcg cggccgcgac ggccgccgag cccaagcgag cctcctcctg gttcgccctc
     4381 gcgcggctcc gcctggtcga ggcggaagac gggaagggcg gcctcgaggc cctcgcgaag
     4441 gccctggagg ccggcttcgc agacaaggcg gcggccgccg ccctccttgg cgagcggaac
     4501 ctcgtcgaga gggaggccgt cgccaaggcc cttcgcgaga agggcctcgc gccttgaggc
     4561 ccaagcggct ccaagccctc gcagcccttg ccctcgtcgc cgcggcctcg gccgcgatag
     4621 cctggtcggc cgcgcgggcc gccgccgcgc gggggcagac cggcgccgaa gtcgcggcgc
     4681 ctgccggctc gcgggccgcc gccgcgggcc cggccgtcca gactgcgccg ccgtccgcgg
     4741 cctcctccgc gcccgaagcg atggcctccg agcccgccgc gcaagccgtc gcgcctaacg
     4801 cgccgctcgg ggctgccgct gcggcaggcg cg
//
LOCUS       DAOOSJ010000207         4785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11164_length_4785_cov_6.073362, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000207 DAOOSJ010000000
VERSION     DAOOSJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11164_length_4785_cov_6.073362"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLG14_09810"
     CDS             complement(<1..868)
                     /locus_tag="PLG14_09810"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX49466.1"
                     /translation="MSASGGRAGEEGAAAQGRAEGLGAGGFGEIARRALAEGLFAGLV
                     LRVERNGGLLFEEAWGEAAASGALGPGSAPVAMATSTVFDLASVSKLFTTTAVLRLVT
                     MGELGLGEDVRGLLSRCSRSLGLAPALLSRLSADLGRVDVAALLSHSSGIHYWFPFYA
                     ARAAADGAGRVAPDGAAAESAAARGAAPAGGAGFEEILASVLEAHPPEGKVVYSDLNF
                     MLLGRLVEGATGLPLPEAVAALVLKPLGLKRTSYAPRRGPGPLGPCAATEFGNRIERG
                     MVAALGLSFDAWR"
     gene            complement(865..1335)
                     /locus_tag="PLG14_09815"
     CDS             complement(865..1335)
                     /locus_tag="PLG14_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49467.1"
                     /translation="MRKEDARACAAMVCASTIGERYGFEPEALAATLASALEGGGELF
                     VAETEGRLGGFAWIDPRGAFSSAPYLRLIAVDESLRGSGIGAALMDEFEARTASVGRD
                     WCLLVSDFNLQAQAFYERRGYRKAGCLPGFARPDIAEILMVKKRPAAAAPGDAR"
     gene            complement(1370..2470)
                     /locus_tag="PLG14_09820"
     CDS             complement(1370..2470)
                     /locus_tag="PLG14_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOX49468.1"
                     /translation="MEIRIFRLRLPLRSAFRIAHGSYAFRENLFLELREGGASGMGEA
                     PIVPYYGLSIDEVEADLRRGLAGFLDALPRGSSAPGGGFGPAAAASARFAHPVGAAAF
                     QAAFLSLGAALEGKSLASFLGLETAPAQADPAAAASPPTSYTIAYDDDPEAMLAAARA
                     SGFRRLKVKAGISGDLERIRRLREGLPEALIRVDANQGWSLEEAPGKLAELERLGVEL
                     VEEPVSGSPSDYERLAAGTSIPILLDESARSLEDAKRYAHGAPSLAGLVVKTAKSGGP
                     AASLALVRAARELGLKVMLSSMVETSLGVGSALPLAPLCAWCDLDAPLLLAEDPFSGL
                     SYEDEVPRLAPGGVRPGAELAARVAALRPLFA"
     gene            complement(2521..3525)
                     /locus_tag="PLG14_09825"
     CDS             complement(2521..3525)
                     /locus_tag="PLG14_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX49469.1"
                     /translation="MSDEGNLLEVRGLKVHFPIHKGVLVRRRAGFVRAVDGVDFSVRK
                     GEALGLVGESGCGKSTTAMAVAQLQAATAGQVLFEGRDLGSLPPRELRRARRDFQLVF
                     QDPYSSLDPRMRAFDIVAEPLRIFARLGAVEADEAEISRRTLSLMERCGLSAGFAKRY
                     PHEFSGGQRQRIGIARALSLGPKLIIADEPVSALDVSIQSQILNLLKDLQKDFGLTFL
                     FIAHDLAVIEYFCDRIAVMYLGRVVETASAEELNAKPLHPYTKALLSAVPLPDPRKER
                     SRRRVILSGDVPSPSVERKGCPFRERCPEAMGRCALEAPSLKGVGEGHEVSCFLFGED
                     "
     gene            complement(3528..4577)
                     /locus_tag="PLG14_09830"
     CDS             complement(3528..4577)
                     /locus_tag="PLG14_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49470.1"
                     /translation="MSATSVISGAKGSAKGSAAAGPQPILAVRDLVTSFRVEGQWRAV
                     VRGVSFEVAPRETVALVGESGSGKSVTALSVMRLTAPGASRVEGSVRLAGRELLTLGD
                     ADIRRVRGNEAAMIFQEPMTSLNPVLTIGFQIAEALILHRGLNRSDAEAETVRLLEKV
                     RIPAARSRLHDYPHRFSGGMRQRVMIAMALACRPKLLIADEPTTALDVTIQAQILELI
                     KSLAARDRTAVIMVTHDLGVVAGMCESVCVMYAGRIVEKALVDDLFYDPKHPYTRGLL
                     DSIPRADRTGKDERLYSIPGSPPSLVGMPDACPFHPRCPRAMDICRKAYPPETRLEGL
                     GPRTVSCWLHAKERA"
BASE COUNT          684 a         1670 c         1798 g          633 t
ORIGIN      
        1 cgcgccaggc gtcgaaggag aggccgaggg cggcgaccat gccccgctcg atgcggttcc
       61 cgaactcggt ggcggcgcag ggtcccaggg ggcccgggcc gcggcggggg gcgtagctcg
      121 tgcgcttaag gccgaggggc ttgaggacga gggcggcgac cgcctccggc agggggaggc
      181 ccgtcgcgcc ctcgacaagg cggccgagga gcatgaagtt gaggtcggag tagaccacct
      241 tgccctccgg cggatgggcc tccaggacag aggcgaggat ctcctcgaag ccggcgccgc
      301 cagcgggagc ggcgccgcgc gcggccgcgc tctccgcggc ggcgccatcg ggtgcgacgc
      361 ggccggcgcc gtccgcggcg gctcttgccg cgtagaaggg gaaccagtag tggatgcccg
      421 aggagtggga taggagggcc gccacgtcga cgcggcccag gtccgccgac agccgcgaca
      481 ggagagcggg cgcgaggccg aggctcctcg agcagcgcga aaggaggccg cggacgtcct
      541 ccccgaggcc gagctctccc atggtgacga ggcggaggac ggccgtggtc gtgaagagct
      601 tggagacgga ggcgaggtcg aagaccgtcg aggtcgccat cgcgacaggc gccgagcccg
      661 gccccagggc gccggacgcg gcggcctcgc cccaggcctc ctcgaacagc aggccgccgt
      721 tccgctcgac gcggaggacc aggcccgcga agaggccctc ggccagggca cgccgcgcga
      781 tttcgccgaa gccgccggct ccgagtccct cggcccggcc ctgcgccgcg gcgccttctt
      841 ctcccgcgcg gccgcctgag gcgctcatcg cgcgtctccg ggcgccgccg cggcgggacg
      901 cttcttcacc atgaggatct ccgcgatgtc cggtcgcgcg aagccgggga ggcaccccgc
      961 cttccggtag ccccgccgct cgtagaaggc ctgggcctgc aggttgaagt cggagacgag
     1021 gaggcaccag tcgcggccga cggaggccgt cctcgcctcg aactcgtcca tcagggccgc
     1081 cccgatcccc gagccccgca gggactcgtc cacggcgatg aggcggaggt atggcgcgct
     1141 cgagaaggcg ccgcgggggt cgatccaggc gaagccgccc agcctgccct ccgtctccgc
     1201 gacgaagagt tcccctcccc cctccagggc ggacgcaagg gtcgcggcca gggcctcggg
     1261 ttcgaagccg tagcgttcgc ctatcgtgga ggcgcagacc atggcggcgc aggccctcgc
     1321 gtcctccttc ctcatggggc ggatcctcgg caccatcgct gtcacctccc taggcgaaga
     1381 gcggacgcag ggccgcgact cgggcggcga gctccgcgcc gggacggacg ccgccgggcg
     1441 cgagcctcgg gacctcgtcc tcgtaggaca ggcccgagaa gggatcctcc gcgaggagga
     1501 ggggcgcgtc caggtcgcac caggcgcaga ggggcgcgag gggcagggcc gagccgacgc
     1561 ccagggaggt ctccaccatc gagctcagca tgaccttgag tccgagctcc cgcgcggcgc
     1621 ggaccagggc caggctggct gcggggcctc cgctcttcgc ggtcttcacg acgaggcccg
     1681 ctagggaggg agctccgtgc gcgtagcgct tcgcgtcctc caggctcctc gcgctctcgt
     1741 cgaggaggat ggggatggag gtcccggccg cgaggcgctc gtagtccgat gggctgcccg
     1801 agacgggctc ctccacgagc tcgacgccga ggcgctcgag ctcggcgagc tttcccggcg
     1861 cctcttcgag gctccagccc tggttggcgt cgacgcggat gagcgcctcg ggcaggccct
     1921 cgcggaggcg ccggatccgc tccaggtcgc ccgagatgcc cgccttcacc ttgagacggc
     1981 ggaagcccga ggccctcgcg gcggcgagca tcgcctccgg atcgtcgtcg taggcgatgg
     2041 tgtaggaggt cggaggcgag gcggccgcgg cagggtccgc ctgggccggg gccgtctcca
     2101 ggccgaggaa ggaggcgagg ctcttcccct cgagcgcggc gcccagggag aggaaggcgg
     2161 cctggaaggc ggccgcgccc acgggatggg cgaagcgcgc ggaggccgcg gccgcggggc
     2221 cgaagccccc tcccggcgcg gaggagcccc ggggcagggc gtccaggaag cccgcgaggc
     2281 cgcggcggag atcggcctcg acctcgtcga tggagaggcc gtagtagggc acgatgggcg
     2341 cctcccccat gcccgaggcc ccgccctcgc gcagctcgag gaagaggttc tccctgaagg
     2401 cgtaggagcc gtgggctatc ctgaaggcgc tccgcaaggg gagcctcagc ctgaagatcc
     2461 tgatttccat ggtgtcctgt cgccttttct gtcccgcccg gccgctcgct gcgcccggcc
     2521 tcagtcttcc ccgaagagga agcaggagac ctcgtggccc tcgccgacgc ccttcaggga
     2581 tggcgcctcg agggcgcagc gccccatcgc ctcggggcag cgctcgcgga agggacagcc
     2641 cttccgctcc acgctcggcg agggcacgtc gcccgagagg atgacgcggc gcctggaccg
     2701 ctccttccgc ggatcgggca ggggcacggc ggagaggagg gccttcgtgt aggggtggag
     2761 gggcttcgcg ttgagctcct ccgccgaggc ggtctccacg acgcggccca ggtacatgac
     2821 ggcgatgcgg tcgcagaagt actcgatcac cgcgaggtcg tgggcgatga agaggaaggt
     2881 gaggccgaag tccttctgca ggtccttgag gaggttgagg atctgggact ggatcgacac
     2941 gtccagggcg gagacgggct cgtcggcgat gatgagcttg ggccccaggg agagggcccg
     3001 ggctataccg atgcgctggc gctggccgcc tgagaactcg tgggggtagc gcttcgcgaa
     3061 gcccgcggag aggccgcagc gctccatgag ggagagggtc ctccttgaga tctccgcctc
     3121 gtccgcctcc acggcgccca gccgcgcgaa gatgcgcagg ggctcggcga cgatgtcgaa
     3181 ggcccgcatc cgcgggtcga gggaggagta ggggtcctgg aagacgagct ggaagtcccg
     3241 cctcgcgcgg cgcagctccc tgggcgggag gctccccagg tccctcccct cgaagaggac
     3301 ctggccggcc gtcgcggcct ggagctgggc gacggccatc gccgtcgtcg acttgccgca
     3361 gcccgactcg cccacgaggc ccagggcctc gcccttcctc acgctgaagt ccacgccgtc
     3421 cacggcccgc acgaagccgg ccctgcgccg gacgaggacg cccttgtgga tggggaaatg
     3481 gaccttgagg ccccgcactt cgaggaggtt tccttcgtcg ctcatggcta tgctctttcc
     3541 ttcgcgtgga gccagcagct caccgtccgg gggcccaggc cctcgagcct ggtctccggc
     3601 gggtaggcct tgcggcagat gtccatcgcg cgcgggcagc gcggatggaa ggggcaggcg
     3661 tcgggcatgc cgacgaggct cgggggcgag cccgggatcg agtagaggcg ctcgtccttc
     3721 cccgtccggt cggcgcgggg gatggagtcg aggaggcccc tcgtgtaggg gtgcttcggg
     3781 tcgtagaaga ggtcgtcgac cagggccttc tccacgatgc ggcccgcgta catcacgcag
     3841 acggactcgc acatgcccgc gacgaccccg aggtcgtggg tgaccatgat gacggcggtg
     3901 cggtccctcg ccgcgaggct cttgatgagc tccaggatct gggcctggat cgtcacgtcc
     3961 agggccgtcg tcggctcgtc ggcgatcagc agcttcggcc ggcaggccag cgccatcgcg
     4021 atcatcacgc gctggcgcat gccgccggag aagcggtgcg gatagtcatg cagccgtgac
     4081 ctggccgccg ggatcctgac cttctccagc agccgcaccg tctcggcctc ggcgtcgctg
     4141 cggttcaggc cgcggtgcag gatcagcgcc tcggcaatct ggaagccgat cgtcagcacc
     4201 gggttcaggc tcgtcatcgg ctcctggaag atcatcgccg cttcgttgcc gcggacccgg
     4261 cggatgtcgg catcgccgag ggtcagcaac tcgcggccgg ccagtcggac gctgccctcg
     4321 acgcggctcg cccccggcgc ggtcagccgc atcaccgaca gggccgtcac gctcttgccc
     4381 gagcctgact cgccgaccag ggcgacggtc tcgcgcggcg cgacctcgaa ggacacgccg
     4441 cgcacgacgg cccgccactg gccttcgacc cgaaacgagg tgaccagatc gcggaccgcg
     4501 aggatcggct gaggtcccgc cgccgcgctc cccttcgcgc tgcccttcgc gccgctgatc
     4561 accgaggtcg cgctcatcga cagctcctcg ccgtcaggct gatcacgggc cgcctcatat
     4621 cacccgccgc gggtcgaggg cgtcgcgcag gccgtcgccg atgaagttga tcgcgagcac
     4681 ggtcaggaag atcgccaggc cggggaacag ggcccagtgc ggggcgacgt cgagatgatc
     4741 cttcgcgtcg aacagcagcc gtccccaggt cgggatgtcg ggcgg
//
LOCUS       DAOOSJ010000208         4784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11166_length_4784_cov_4.812434, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000208 DAOOSJ010000000
VERSION     DAOOSJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11166_length_4784_cov_4.812434"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLG14_09835"
     CDS             <1..384
                     /locus_tag="PLG14_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49471.1"
                     /translation="RIEEGIVHALEKLDAFEDLVIRAAEPSPREVPAADRRPAPAREA
                     PAAPAQAAAPAAVPLREAAPAAHVAVAPAPAQAAALAAAPALDADELSLEELEAATAP
                     DAPVAKAPAAQPAAQPAPANELDIF"
     gene            404..799
                     /locus_tag="PLG14_09840"
     CDS             404..799
                     /locus_tag="PLG14_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOX49472.1"
                     /translation="MPVQLISIEVLGASFSVQTDETREYMETLLAELERRLDALRKAT
                     KVADPLKLAILANITLLDELARARRETGGGQASEELGRIAERLIGRLDEGLAASGAGD
                     GASVAGAVPGAASSAAQEGAEPSNPVSPV"
     gene            878..1069
                     /locus_tag="PLG14_09845"
     CDS             878..1069
                     /locus_tag="PLG14_09845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOX49473.1"
                     /translation="MVMPLEQLIEYDANAYEFTVAVTHRAYQLAVIKGPEVEKFNSKV
                     VSLSTRQIFTKQVEYRLLD"
     gene            1047..2057
                     /gene="miaA"
                     /locus_tag="PLG14_09850"
     CDS             1047..2057
                     /gene="miaA"
                     /locus_tag="PLG14_09850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOX49474.1"
                     /translation="MSTASSTRIPVLVLAGPTASGKTDLLLELFGGGGRSVFPPAEIV
                     SADSMQAYRGMDIGTAKPDAALRASLPHRLVDILDPAEQYSVGDFVRLADEACAQIAA
                     GGGLPVVSGGTGFYIRNFMLGLPTSPVSDPAMREAVARDFAEKGRAALRAELEALDPA
                     AAARIHPNDSYRLQRALEILRATGRPLSDFAPPGLGAADLGEGLGEGRPAYRFLVACV
                     ERPREELYARIDARVDAMFRAGLPAEFARLRAAGYGREAPGMKAIGYSEFFEAEAEGA
                     DLRSEAGLARVAEAVKLHSRRYAKRQETFFRGLPGLVRVPAQGAAAALSSLLAAFLAK
                     GV"
     gene            2129..2608
                     /locus_tag="PLG14_09855"
     CDS             2129..2608
                     /locus_tag="PLG14_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOX49475.1"
                     /translation="MKASTLLVSAILSLTPISELRGAIPYAVAAGMPLVAAALWCVAW
                     NALAAPLAYLFLATFHKLFYRFKPYRNFFDRFVEKARGKVHASVEKYGYLGLAVFVGV
                     PLPVTGAWTGALGAWILGMDRKKSVLAIVLGVAAAGAIVSLVVGLGVGALSIFVKRV"
     gene            2619..4679
                     /locus_tag="PLG14_09860"
     CDS             2619..4679
                     /locus_tag="PLG14_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX49476.1"
                     /translation="MAIDYASELNPEQYEAATTIDGPLLIIAGAGSGKTRVITFRMAY
                     MLDKGIPQSSILALTFTNKAAKEMAERVKSVTKRKLKNLTVSTFHSFGVKILRKEIEA
                     LGWRENFSIYDEADRYQLIKDCARDLGFRMEAFDPVKAGALFSDIRTERRPWSKADDA
                     YRQLYDEYRRSLKIYNSVDFDDLIVMPTEIFGLHPDIRAKYREQYRYVMIDEFQDTSL
                     IQYKMMRLLCDRNVCVVGDDDQSIYSWRGANYENLMQFERDFPDVKEIKLERNYRSTS
                     TILDAANAVIRNNTSRKEKALWSPAGLGGAPIELHLPEDEMAEAEFIARTIKAIKLED
                     HVPWDEFGILIRTNALTRWLEEGLLAENVPYRISGGTSFFGRKEIKDVISYLRVIANP
                     DDDVNLIRILNTPRRGIGKTTLERLGNVARERKCSIRTAMELARAGESLGAEGGAEGG
                     LAFGGLSAKTIADVEAFLDLVAEYREEMLGKKRIAEKVRKLMDQIDYWGYLIEENKAN
                     DKVAKWKFHNVELFIQMIDRWEKDPDNLSPGLYEWLNRISLITRDDLSEEDEGKVNLM
                     TIHAAKGLEFDVVFIAGCEDGIIPHAKSLEEGEGNLEEERRLFYVAVTRARRRLMISA
                     ALRRRKQNQATDQVPSPFLKEIPESLITVVESEAEFTEEEGDAYFGKLQAMFAPPPGT
                     EDSA"
BASE COUNT          778 a         1815 c         1509 g          682 t
ORIGIN      
        1 cgcatcgagg agggcatcgt ccacgccctc gagaagctcg acgccttcga ggacctcgtg
       61 atccgcgcgg ccgagccttc ccccagggaa gtcccggccg cggatcgccg cccggccccc
      121 gcccgcgagg cgcccgcggc ccccgcccag gcggccgctc ccgcggcggt ccccctccgc
      181 gaggcggccc cggccgccca cgtggccgtc gctccggccc ccgcccaggc ggccgccctt
      241 gcggcggcgc ccgccctcga cgccgacgag ctcagcctcg aggagctcga ggccgcgacg
      301 gcccccgacg cgcccgtggc caaggcgccc gccgctcaac cggccgccca gcccgctccc
      361 gcgaacgagc tcgacatctt ctaggccaag ggcgcggtag gccatgcctg tccagctcat
      421 cagcatcgaa gtcctcggcg cctccttctc agtgcagacc gacgagaccc gcgaatacat
      481 ggagaccctc ctcgccgagc tggagcgccg cctggacgcc ctgcgcaagg ccacgaaggt
      541 cgccgacccc ctgaagctcg caatcctcgc caacatcacc ctcctcgacg agctcgcgcg
      601 cgcgaggcgg gagacgggcg ggggccaggc ctccgaggag ctcggacgca tcgccgagcg
      661 cctcatcggc cgcctggacg aggggctcgc cgcctccgga gccggggacg gagcttcggt
      721 cgcgggcgcc gtccccggag cagcctcaag cgccgcccag gagggcgcgg agccttccaa
      781 tccagtctcc ccggtctaaa ggccggtcca gggacttgat ggcgcgggac tttttgctgt
      841 agactctttc ccactacgca gttacgagga gcccggcatg gtcatgcctc tcgagcagct
      901 cattgaatac gacgccaacg cctacgagtt caccgtcgcc gtcacccatc gcgcctacca
      961 gctggccgtg atcaagggac ccgaggtcga gaagttcaac agcaaggtcg tctccctctc
     1021 cacccgccag atcttcacga agcaggttga gtaccgcctc ctcgactagg atccccgtcc
     1081 tcgtcctggc cgggcccacg gcctccggca agaccgacct cctcctcgag cttttcggcg
     1141 ggggaggtcg ttccgtcttt cctcccgcgg agatcgtgag cgccgactcg atgcaggcct
     1201 accggggcat ggacatcggc acggccaagc ccgacgccgc cctccgcgcg agccttcccc
     1261 atcgccttgt cgacatcctc gaccccgcgg agcaatactc ggtcggcgac ttcgtccgcc
     1321 tggccgacga ggcctgcgca cagatagccg ccggcggcgg cctgcccgtc gtctcaggcg
     1381 gcaccggctt ctatatccgc aacttcatgc tcggcctacc cacctccccc gtctccgatc
     1441 cggccatgcg ggaggcggtg gcccgcgact tcgcggagaa aggccgggcg gccctgcggg
     1501 ccgagcttga ggccctggac ccagccgcgg ccgcgaggat ccacccgaac gacagctatc
     1561 gcctccagcg cgccctcgag atcctccggg cgacggggag gcccctctcc gacttcgcgc
     1621 cgccgggcct cggcgccgcc gatctcggcg agggcctcgg cgagggccgg cccgcctacc
     1681 gcttcctcgt cgcctgcgtg gagcggcccc gcgaggagct ctacgcccgc atcgacgccc
     1741 gcgtggacgc catgttccgc gccggcctgc ccgccgagtt cgcccggctc cgcgccgcgg
     1801 gctacggacg ggaggctcct ggcatgaagg ccatcggcta ctcggagttc ttcgaggccg
     1861 aggccgaggg cgccgacctc cgctccgagg ccggcctcgc gcgggtcgcc gaggcggtga
     1921 agctccactc gcgccgctat gccaagcgcc aggagacctt cttccgcggc cttccgggcc
     1981 tggtccgcgt ccccgcgcag ggcgcggccg cggccctctc ctccctcctt gcggccttcc
     2041 tggcgaaagg cgtctagccc cgcccggccc tggccaatcc ggcgctccgc cgtgacatgg
     2101 tgaggcctcc caggctatag tggcgccaat gaaagcctcg accctcctcg tctccgccat
     2161 cctctccctg acgcccatct ccgagctcag gggggccata ccctacgccg tcgcggcggg
     2221 catgcccctc gtcgccgcgg ccctctggtg cgtcgcctgg aacgccctcg cggcgccgct
     2281 ggcctacctc ttcctcgcga ccttccacaa gctcttctac cgcttcaagc cctaccggaa
     2341 cttcttcgac cgcttcgtgg agaaggcccg cggcaaggtc cacgcgagcg tcgagaagta
     2401 cggctacctg ggactggcgg tcttcgtcgg cgtgcccctc cccgtcaccg gggcctggac
     2461 cggcgccctg ggcgcctgga tcctcggcat ggaccggaag aagtccgtcc tcgccatcgt
     2521 cctgggcgtc gcggccgccg gagccatcgt gagcctcgtc gtcggcctgg gcgtcggagc
     2581 cctctccatc ttcgtgaagc gcgtctagca aggaacccat ggcgatagac tacgcctcgg
     2641 agctcaaccc cgagcagtac gaggccgcga cgacgatcga cggccccctc ctcatcatcg
     2701 cgggcgcggg ctcggggaag acccgcgtca tcaccttccg catggcctac atgctcgaca
     2761 agggcatccc ccagagctcc atcctggccc tcaccttcac gaacaaggcg gccaaggaga
     2821 tggccgagcg cgtgaagtcc gtcacgaagc ggaagctcaa gaacctcacc gtctccacct
     2881 tccactcctt cggcgtgaag atcttgcgga aggagatcga ggccctgggc tggcgcgaga
     2941 acttctccat ctacgacgag gcggaccgct accagctcat caaggactgc gcccgcgacc
     3001 tgggcttccg gatggaggcc ttcgaccccg tgaaggccgg cgccctcttc tccgacatcc
     3061 gcaccgagcg ccgcccctgg tccaaggccg acgacgccta ccgccagctc tacgacgagt
     3121 accgccgctc cctcaagatc tacaactccg tcgacttcga cgacctcatc gtgatgccca
     3181 cggagatctt cggcctccac cccgacatca gggccaagta ccgcgagcag taccgctacg
     3241 tgatgatcga cgagttccag gacacctcgc tcatccagta caagatgatg cgcctcctgt
     3301 gcgaccggaa cgtctgcgtc gtcggcgacg acgaccagtc catctactcc tggcgcggcg
     3361 cgaactacga gaacctcatg cagttcgagc gcgacttccc cgacgtgaag gagatcaagc
     3421 tcgagcggaa ctaccgctcc acctccacga tcctggacgc ggccaacgcg gtgatccgga
     3481 acaacacgag ccgcaaggag aaggccctct ggtcgcccgc gggcctcggg ggcgcgccca
     3541 tcgagctcca cctcccggag gacgagatgg cggaggccga gttcatcgcg cggaccatca
     3601 aggcgatcaa gctcgaggac catgtcccct gggacgagtt cggcatcctc atccggacga
     3661 acgccctcac gcggtggctg gaggagggcc tcctcgcgga gaacgtgccc taccgcatct
     3721 ccggcggcac ctccttcttc ggccgcaagg agatcaagga cgtcatctcc tacctccgcg
     3781 tgatcgcgaa tcccgacgac gacgtgaacc tcatccgcat cctcaacacc ccgcgccgcg
     3841 gcatcgggaa gacgaccctg gagcgcctgg ggaacgtcgc ccgcgagcgc aagtgctcca
     3901 tccgcaccgc gatggagctc gcgcgggcgg gcgagagcct cggggccgag ggcggcgcgg
     3961 aggggggact ggccttcggc ggcctctccg cgaagaccat cgccgacgtg gaggccttcc
     4021 tggacctggt cgcggagtac cgcgaggaga tgctcgggaa gaagcgcatc gccgagaagg
     4081 tccgcaagct catggaccag atcgactact ggggctacct catcgaggag aacaaggcga
     4141 acgacaaggt cgccaagtgg aagttccaca acgtcgagct cttcatccag atgatcgacc
     4201 gctgggagaa ggaccccgac aacctctccc ccggcctcta cgagtggctc aaccgcatct
     4261 ccctcatcac ccgcgacgac ctgtcggagg aggacgaggg caaggtcaac ctcatgacca
     4321 tccacgccgc gaagggcctg gagttcgacg tcgtcttcat cgcgggctgc gaggacggca
     4381 tcatccccca cgcgaagagc ctggaggagg gggaggggaa cctggaggag gagcggcggc
     4441 tcttctacgt cgcggtgacg cgggccaggc gccgcctcat gatctccgcc gccctgcgcc
     4501 ggcgcaagca gaaccaggcg acggaccagg tcccgagccc cttcctgaag gagatcccgg
     4561 agagcctcat caccgtcgtc gagtcggagg ccgagttcac ggaggaggag ggcgacgcct
     4621 acttcgggaa gctccaggcc atgttcgcgc cccctcccgg cacggaggac agcgcctagg
     4681 ccgggagcgg aggccgggcg gcgaggttgc ggcgcgcaga aacgccgtgc tacgatagcg
     4741 gcacgcgcgc cggggagtca accggaggcg cttggaatgc cggc
//
LOCUS       DAOOSJ010000209         4672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11525_length_4672_cov_6.239116, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000209 DAOOSJ010000000
VERSION     DAOOSJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11525_length_4672_cov_6.239116"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PLG14_09865"
     CDS             complement(<1..1427)
                     /locus_tag="PLG14_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOX49477.1"
                     /translation="MGSAEGSEARLRGDAEAIFRAALDRVEPARMVRRALSLEEGQGG
                     ASLVARTGLEEASYRLADFDRITCLAFGKAAAPMARGLEEALAALEAGGPGPARTAAA
                     RADARPKAPAPAWPVFAGGVAAAKKGHGGSLAKVRLVEAGHPVPDESSVAAARALLEA
                     GSGLTERDLVVVLVSGGGSAILCAPAEGLSLADKAETTRLLLASGATIQEANCVRKHL
                     SAVKGGRLAAALAPASVLALVLSDVVGDDLDAIASGPTVPDPTSFADALAVARRRGIV
                     DRLPPPVRARLEAGAAGRIPDTPKPGDPAFARTRTLLVGTNRLALMAAEEKARELGYA
                     TLALSSRVTGEAREAALFYLGIGKDLAASGFPLKRPACLLAGGETTVTLRGKGRGGRN
                     QEMALAFLAALGRAPKDSGGLLFLAASTDGNDGPTDAAGAFASLGLLEAAREAGLDPE
                     AYLAENDSYAFYERLGGLLKTGPTNT"
     gene            complement(1427..2200)
                     /locus_tag="PLG14_09870"
     CDS             complement(1427..2200)
                     /locus_tag="PLG14_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49478.1"
                     /translation="MTSFYERMGLNAYVQGDYAKAEAWFRKLESREPDSLRVLRNLGV
                     ILLARGDAEGAEAYLLREERAYGPSFHRHAALADLAYARGRREEAARRYSAALAEPEA
                     GGAPTAALIEARARICRDEKAFSRSRDSLSRFAEGEEARGGGDLARAVALFDEAAALD
                     PTAWPALNNAGTLLLNDLGDPEGAKARFERALALSRAPQVARNLELAEGALERERYRS
                     LKAAAAEEAGKGGRGAAGAGKGGKDGKGGKPARAGGEGR"
     gene            complement(2235..3566)
                     /locus_tag="PLG14_09875"
     CDS             complement(2235..3566)
                     /locus_tag="PLG14_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOX49479.1"
                     /translation="MVSGGLALIYLVLAVAVIIVLTGKYKVNAFLVLIGVAFAYGFAI
                     GLPALDVVKHIKNGFGGTLTKIGIVIVAGTIMGTILERTGAALAMTEAILRLVGKKRA
                     PLAMNIAGYVVSIPVFCDSGYVILNPLNKALAKETGTSMAVMAVALSTGLYATHVMVP
                     PTPGPLAAAAALNADLGKVIVLGLVAAIPAALAGLFWALKVAKKYQIEADIKESYSDI
                     VKKYGKLPNTFLSFMPIVMPLVFIFAKSVAEYPTKPFGTGFLQQACSFIGDPVVALML
                     GVLISLLLVRKGGIHEAVNGWMGEGIKDSALILVITAAGGSFGEILGKSPITGFIQTN
                     MAGMNLGIFLPFLIAAALKTAQGSSTVAIVTTAGILAPMLATLGLDPALTALAIGAGS
                     MVVSHANDSYFWVVSQFSGMPVSTAYKVYTSATAVEGVVAVAAIWVMSFIF"
     gene            complement(3823..>4672)
                     /locus_tag="PLG14_09880"
     CDS             complement(3823..>4672)
                     /locus_tag="PLG14_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX49480.1"
                     /translation="VELELYKEKAMRRQDRKNLFVSQLVYEPDAPRSQLEALARKLGY
                     DPRAPRAPLILRPAPGVEPTRALSAIKADGVHGAEDISWVSPEGSIVVWKRLELGGEG
                     LMESIRGQVEAYLLAAGKALGEGPSAGASTGGPAAGGPFLAYSGALQSDLGRYRGGYR
                     QLLWLVERFPSPPGPHVSLYDHLGEFLASRIPRADLVDALDGVAALLPGDFRGGLRDS
                     VEALFESAFNGKEAAARLGVHRNTLSARMERLNRVFGLDPRRDPKAREFLSFLVRYLE
                     LKGPSA"
BASE COUNT          651 a         1569 c         1817 g          635 t
ORIGIN      
        1 gtgttcgtcg gcccggtctt gaggaggccg cccagcctct cgtagaaggc gtaggagtcg
       61 ttctccgcga gataggcctc ggggtccagt ccggcctcac gcgccgcttc gaggaggccg
      121 aggctcgcga aggcgccggc cgcgtcggtc ggcccgtcgt tgccgtcggt ggaggccgcg
      181 aggaagagga ggccccctga gtccttgggc gcgcggccca gggccgccag gaaggccaga
      241 gccatctcct ggttcctgcc gcccctgccc ttgccgcgca gggtgaccgt cgtctctccg
      301 ccagcgagga ggcaggcggg ccgcttgagg gggaagcccg aggcggcgag gtccttgccg
      361 atgccgaggt agaagagggc ggcctcgcgc gcctcgcccg tcacgcggga ggagagggcg
      421 agggtcgcgt agccgagctc gcgggccttc tcctccgcgg ccatgagggc caggcggttc
      481 gtgccgacga ggagggtcct cgtccgcgcg aaggccgggt cgcccggctt gggcgtgtcg
      541 gggatcctcc ccgcggctcc cgcctccagg cgggcgcgga ccggcggcgg gagcctgtcc
      601 acgatgccgc gccggcgcgc gaccgccagg gcgtcggcga agctcgtcgg gtcgggcacg
      661 gtgggccccg aggctatggc gtccaggtcg tcgccgacga cgtcggagag gacgagggcg
      721 aggacggagg ccggcgcgag ggcggcggcc aggcggccgc ccttcaccgc ggagaggtgc
      781 tttcggacgc agttcgcctc ctggatcgtc gccccggagg cgaggaggag gcgcgtcgtc
      841 tccgccttgt cggccaggct gagaccttcg gccggagcgc agaggatggc cgagccgccg
      901 cccgagacga ggacgacgac gaggtcgcgc tccgtgaggc cggagcccgc ctccaggagg
      961 gcgcgggccg cggccacgga gctctcgtcg gggacgggat ggccggcctc gacgaggcgg
     1021 accttcgcga ggctcccgcc gtggcccttc ttggccgcgg ccacccctcc ggcgaagacc
     1081 ggccacgcgg gcgccggggc cttcggccgc gcgtcggccc gggccgccgc ggtccgggcc
     1141 gggccgggcc cgccggcctc cagggcggcc agggcctcct cgaggccgcg ggccatgggg
     1201 gcggccgcct tcccgaaggc caggcaggtt atgcggtcga aatcggcgag ccggtagctc
     1261 gcctcctcga ggcccgtgcg ggcgacgaga ctggcgccgc cttgcccctc ctcgagggag
     1321 agggcgcggc ggaccatcct ggcgggctcg acgcggtcca aggccgcccg gaagatggcc
     1381 tccgcgtccc cgcggagcct cgcctcggag ccctcggcgg agcccatcaa cgaccctccc
     1441 cgccggcccg ggcaggcttg ccgcccttgc cgtccttgcc gcccttgccg gcaccggccg
     1501 cgccgcgccc gcccttgccg gcttcctcgg ccgcggcggc cttgaggctc cgatacctct
     1561 cgcgctcgag ggcgccctcg gcgagctcga ggttccgcgc cacctggggc gcgcgggaga
     1621 gcgcgagggc ccgctcgaag cgggccttcg cgccctcggg atcgcccagg tcgttgagga
     1681 ggagggtgcc cgcgttgttg agggcgggcc aggccgtcgg atccagggcc gcggcctcgt
     1741 cgaagagggc caccgcgcgg gccaggtcgc ccccgccgcg ggcctcttcg ccctcggcga
     1801 agcgggagag ggagtcgcgg ctcctcgaga aggccttctc gtcgcggcag atgcgcgcgc
     1861 gggcctcgat gagggcggcc gtgggcgcgc ccccggcctc cggctcggcc agggcggccg
     1921 agtagcgccg cgccgcctct tcccgccttc cccgcgcgta ggcgaggtcg gccagggcgg
     1981 cgtggcggtg gaagctcggg ccgtaggccc gctcctcgcg gaggaggtag gcctcggcgc
     2041 cctcggcgtc ccccctcgca aggaggatga cgccgaggtt ccgcaagact cggagggaat
     2101 cgggctcgcg ggactcgagc ttccggaacc aggcctcagc cttggcgtag tcgccctgga
     2161 cgtaggcgtt gagtcccatc cgctcgtaga aagacgtcac gatgttcggc atagagcccg
     2221 gttcccccgc gttcctagaa tatgaagctc atgacccaga tcgcggcgac ggcgacgacg
     2281 ccctccaccg cggtcgccga ggtgtagacc ttgtaggccg tcgacaccgg catgcccgag
     2341 aactgcgaga cgacccagaa gtaggagtcg ttcgcgtggg agactaccat cgagcccgct
     2401 ccgatggcga gggccgtcag ggcggggtcc aggccgaggg tggcgagcat cggggccagg
     2461 atgccggccg tcgtgacgat cgcgacggtg gaggagccct gggcggtctt gagggccgcg
     2521 gcgatgagga aggggaggaa gatgccgagg ttcatcccgg ccatgttggt ctggatgaag
     2581 cccgtgattg gcgacttccc gaggatctcg ccgaaggagc cgcccgcggc ggtgatgacg
     2641 aggatgaggg ccgagtcctt gatgccctcg cccatccagc cgttcacggc ctcgtggatc
     2701 ccgcccttcc tgacgaggag gagggagatg aggacgccga gcatgagagc cacgacgggg
     2761 tctccgatga aggagcaggc ctgctggagg aagcccgtgc cgaagggctt ggtcgggtac
     2821 tccgcgacgg acttcgcgaa gatgaagacc aggggcatga cgatgggcat gaaggagagg
     2881 aaggtgttcg ggagcttccc gtacttcttc acgatgtcgg agtagctctc cttgatgtcc
     2941 gcctcgatct ggtacttctt cgcgaccttg agggcccaga agaggccggc cagggccgcg
     3001 gggatcgcgg ccacgaggcc caggacgatg accttcccga ggtccgcgtt gagggcggcg
     3061 gcggcggcga gggggcccgg ggtgggcggc accatcacgt gggtggcgta gaggccggtg
     3121 gagagggcga cggccatcac ggccatggag gtgcccgtct ccttggcgag ggccttgttg
     3181 agggggttga ggatgacgta gccggagtcg cagaagacgg ggatggagac cacgtagccc
     3241 gcgatgttca tcgcgagggg ggcgcgcttc ttcccgacga gcctgaggat ggcctccgtc
     3301 atcgcgaggg cggcgcccgt cctctccagg atggtgccca tgatggtgcc ggccacgatg
     3361 acgatgccga tcttcgtgag cgtgccgccg aagccgttct tgatgtgctt gacgacgtcg
     3421 agggccggca ggccgatcgc gaagccgtag gcgaacgcga cgccgatgag gacgaggaag
     3481 gcgttgacct tgtacttgcc ggtcaggacg atgatgaccg cgacggcgag gacgaggtag
     3541 atgagggcta ggcctccgct taccatggga acctccttga agggacgtgg gatcgagtag
     3601 agggtgcacc cgtttcccgg aacgcgaaat ggacgcgggg gcgaatccgc gggccgctcc
     3661 ctgtgcacgg tgcacaacgg cggctcgccc gggcgcgggc gggcgcgcgt cgctaaggcc
     3721 tggaacgcgc gggcgggcgc tcggagacga ggccttggac gcgccggcgc gcctgcgtgg
     3781 cgaagcttca atgggccgac ggctcagccg cggcggccga gactaggccg aggggccctt
     3841 gagctcgagg tagcgcacca ggaaggagag gaattcccgc gccttgggat cgcgccgggg
     3901 atcgaggccg aagacgcggt tcaggcgctc catccgcgcg gaaagggtgt tccggtggac
     3961 gcccaggcgg gcggcggcct ccttcccgtt gaaggcgctc tcgaagaggg cctccaccga
     4021 gtccctgagg ccgccccgga aatcgccggg caggagggcg gcgacgccgt ccagggcgtc
     4081 gaccaggtcg gccctcggga tccggctcgc caggaactct cccaggtggt cgtagaggga
     4141 gacgtggggc cctggcgggc tcgggaagcg ctcgacgagc cagaggagct ggcgatagcc
     4201 gccccggtag cgccccaggt cggactgcag ggcccccgag taggcgagga aggggccgcc
     4261 ggccgccggc ccgcctgtgg acgcgcctgc cgacggccct tcgcccaggg ccttcccggc
     4321 cgcgaggagg taggcctcca cctggccgcg gatcgactcc atgaggccct cgccgccgag
     4381 ctccagccgc ttccagacga cgatcgagcc ctcgggcgag acccaggaga tgtcctccgc
     4441 gccgtggacg ccgtccgcct tgatcgccga aagggccctg gtcggctcca cgccgggcgc
     4501 tggcctcagg atgagcggag cccgcggcgc cctcgggtcg tagccgagct tcctcgccaa
     4561 ggcctcgagc tgggacctgg gcgcgtccgg ctcgtagacg agctggctca cgaagagatt
     4621 cttgcggtcc tggcggcgca tcgccttttc cttatagagc tcgagctcga cc
//
LOCUS       DAOOSJ010000210         4670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11534_length_4670_cov_5.143229, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000210 DAOOSJ010000000
VERSION     DAOOSJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11534_length_4670_cov_5.143229"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /gene="miaB"
                     /locus_tag="PLG14_09885"
     CDS             complement(<1..1277)
                     /gene="miaB"
                     /locus_tag="PLG14_09885"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOX49481.1"
                     /translation="MKQYFIETYGCQMNKAESAALEVIFRERGWSPAESPESAALVLI
                     NTCSVRITAENRAWGRIDHFAALKRERAQAGGSGPALVVTGCMAERMKRGIKERQPAV
                     DYVVGTFQKQAFGLVLDAVAAGRALGDIEESPAFVFAKSYYEPGSFRSFLPIMHGCDN
                     FCSYCIVPYLRGRETSRKPEDILSELDALEDSGVREVTLLGQNVNSYRYKGTASGGEA
                     LDLDFPALLRLVARHLKERGRGGIRWIRFLTSHPKDLSDDLVAAIAEEELFCRHIHLC
                     VQSGSDRILKAMNRKYDTAYYLSLVERLKSAIPGLTLSTDLLVGFPGETEEDNELTLG
                     LMRRVGFSYAYMYHYNPREGTPAATMPGRVAEKVKKARLARVIELQKEISRRLMESRV
                     GAVEEVLVEGVSRRRKSEVLARTQADEMVVFPAP"
     gene            complement(1480..1893)
                     /locus_tag="PLG14_09890"
     CDS             complement(1480..1893)
                     /locus_tag="PLG14_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49482.1"
                     /translation="MSDLEPIGSKEITNSGVRGIGATVGGLALFFLRGLSTAFGGFLG
                     LGLGALSFIVGASSVKSASKADKVGGSIAMGAGIVLALPGAARLLHVVPVVGGILGVA
                     AGFSGWVIGAGALALVGYGVFNIVKFVRGIRSHRG"
     gene            complement(2126..3418)
                     /locus_tag="PLG14_09895"
     CDS             complement(2126..3418)
                     /locus_tag="PLG14_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOX49483.1"
                     /translation="MKTPSEKPSLQSAYRSFAEDRFPPRMEIAFYDETGRRTALSYEK
                     LTWEVEGERKGLRYGENPDQGAALYRPVRGNLALGGAEFLGEGSEGGLPAPGGLVAAA
                     ELLQFGKHPGKTNLTDVDAALNILRYLSAGPACAIMKHNNPSGVARAATLAEAYGRAY
                     LADRVAAFGGAVVVNRALDLDTARLVASAYCEVVAAPDFEEGVLAELAKRKNLRIMRI
                     PEMARLERWAPARVLDFRALLDGSLVVQTSFASRVLSRGDFLPAEGSHQGKDYRVARE
                     PTDAEWEDMLFGWLVETGVTSNSVIYVKDGATVGIGTGEQDRVGVAEIARDKAYRKLE
                     DRLLWERTGKGWNESADEAAKAEVRAEAASRRGGLAGSVMVSDAFFPFRDGIEVGLRE
                     GVAAVVQPGGSLRDFESIEACNEFGAAMKFTGQRCFRH"
     gene            complement(3547..4248)
                     /locus_tag="PLG14_09900"
     CDS             complement(3547..4248)
                     /locus_tag="PLG14_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49484.1"
                     /translation="MAFNRVEEVQDLVPLRRALVSVYDKDGLPRLIRGLALAAPGLEI
                     YSTGGSYAAVAEVLASLDPAPGQAPEPRLVAVSEYTGQPEMEGGLVKTLDWKIYLGLL
                     AESGNAAHAADLERTGAVAFDLVVGDLYPFEAACRDPSSEPEALRQRIDIGGPAMLRA
                     AAKNYLRVASVSSPGQYAGLLEELRVRGGHTSLDYRRRLAAAAFDRVSRFDRAVSSRL
                     GAMTAEELKAGYRGL"
     gene            complement(4313..>4670)
                     /locus_tag="PLG14_09905"
     CDS             complement(4313..>4670)
                     /locus_tag="PLG14_09905"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HOX49485.1"
                     /translation="PCSKAISAYGAHNQRGVVTLKVALGPFFWIEDLVGLVEGSASSG
                     VYTLLKRDDEKYVTERSFENPRFVEDVVREVYRQVEALGKFPRFSVEAENFESIHNHS
                     AYAYVEYPSGRPSGED"
BASE COUNT          671 a         1555 c         1767 g          677 t
ORIGIN      
        1 ggggcgggga agaccaccat ctcgtcggcc tgggtccgcg cgaggacctc gctcttccgc
       61 cgccgggaga cgccctccac gaggacctcc tcgaccgccc cgacgcgcga ctccatgagt
      121 ctcctcgaga tctccttctg gagctcgatc accctggcga ggcgggcctt cttgaccttc
      181 tccgccacgc ggccgggcat ggtggcggcg ggcgtgcctt cgcggggatt gtagtggtac
      241 atgtaggcgt agctgaagcc gacgcggcgc atgaggccca gcgtgagctc gttgtcctcc
      301 tcggtctcgc cggggaagcc cacgaggagg tcagttgaga gggtgaggcc ggggatggcc
      361 gacttgagcc tctcgacgag ggagaggtag taggccgtgt cgtacttgcg gttcatcgcc
      421 ttgagaatcc ggtcggagcc cgactggacg cagaggtgga tgtgacggca gaagagctcc
      481 tcctccgcga tcgccgcgac gaggtcgtcg gagaggtctt tcgggtggct cgtgaggaag
      541 cggatccagc ggatccctcc cctcccccgc tccttcaagt gcctcgcgac gagccggagc
      601 agggccggga agtcgaggtc cagggcctcg ccgcccgaag ccgtcccctt gtagcggtag
      661 gagttgacgt tctggccgag gagggtcacc tcgcgcacgc ccgagtcctc cagggcgtcg
      721 agctcggaga ggatgtcctc gggcttcctc gaggtctcgc ggccccggag gtaggggacg
      781 atgcagtagg agcagaagtt gtcgcagccg tgcatgatgg gaaggaagga gcggaaggag
      841 cccggctcgt agtaggactt cgcgaagacg aaggcggggc tctcctcgat gtcgcccagg
      901 gccctgcccg ccgcgacggc gtccaggacg aggccgaagg cctgcttctg gaaggtgccg
      961 acgacatagt ccacggccgg ctgcctttcc ttgatcccgc gcttcatgcg ctcggccatg
     1021 cagcccgtga cgacgagagc cgggcccgag ccgcctgcct gggccctctc ccgcttcagg
     1081 gcggcgaagt gatcgatgcg gccccaggcg cggttctccg cggtgatccg gaccgagcag
     1141 gtgttgatga ggacgagggc ggcggactcg ggcgactcgg ccggcgacca gccgcgctcg
     1201 cggaagatca cctcaagggc ggccgactcg gccttgttca tctggcaacc gtaggtttcg
     1261 atgaagtact gcttcataag ggacccacaa tgtaccccga ccgccgcgcc gccttcaacg
     1321 ggttcgccgg agcgtgaaaa ggccgcgccg atagtggcgc ggccttgcct tcgcgcggcc
     1381 ttgccttcgc gcggccttgt tcggccgggg gcggccccat tccgcgcggg cgcggccttc
     1441 ctgcccgcgg ggcgcggtcc gcgccctcgg ctgagccgcc tagccccggt gggagcggat
     1501 gccgcggacg aacttgacga tgttgaagac gccgtagccc acgagggcca gggcgcccgc
     1561 gccgatgacc cagcccgaga agccggcggc gacgcccagg atgccgccga cgacgggcac
     1621 gacgtggagg agcctcgcgg ctccggggag ggccaggacg atgccggctc ccatggctat
     1681 cgagccgccg accttgtcgg ccttggaggc cgacttgacg ctcgaggcgc cgacgatgaa
     1741 ggagagggcg ccgaggccca gtcccaggaa gccgccgaag gccgtggaaa ggccccgtag
     1801 aaagaacagg gccaggccgc ccacggtggc gccgatgccc ctgacgcccg agttggttat
     1861 ttccttgctc ccgatcggct cgagatcgct catggatagc ctccgttcac cgccgcgaca
     1921 tgtttggccc tccccgggag ggcgagggcg ccgggcgcga gtgtcgcgtc aataatacgg
     1981 aattggggcc gcggggtaaa gtggaggggc gcgggtcccg ggccgcgcga ccggcgccgg
     2041 ccagaggcag cacgctagag gcggcaggtc caaggcgggc cgtcggcggc tggacacctg
     2101 acgcgccgtg cctctgccgc ggccttcagt gcctgaagca gcgctggccc gtgaacttca
     2161 tcgcggcgcc gaactcgttg caggcctcga tcgactcgaa gtcgcgcaag ctgccccccg
     2221 gctggaccac ggccgcgacg ccttcgcgga ggccgacctc gatgccgtcg cggaagggga
     2281 agaaggcgtc ggagaccatc acggagcccg cgaggccgcc gcggcgcgag gccgcctcgg
     2341 cgcggacctc ggccttcgcc gcctcgtccg ccgactcgtt ccagcccttg ccggtccgct
     2401 cccagaggag cctgtcctcg agcttgcggt aggccttgtc gcgggcgatc tccgcgaccc
     2461 cgaccctgtc ctgctctccc gtgccgatgc cgaccgtcgc cccgtccttc acgtagatca
     2521 ccgagttgga ggtgacgccc gtctccacga gccagccgaa gagcatgtcc tcccactcgg
     2581 cgtcggtcgg ctcgcgcgcc acgcggtagt ccttgccttg gtgcgagccc tccgccggaa
     2641 ggaagtcgcc gcgggagagg acgcgggagg cgaaggaggt ctggacgacc agggagccgt
     2701 ccaggagggc gcggaagtcc aggacgcggg ccggggccca gcgctcgagc ctcgccatct
     2761 ccgggatgcg catgatgcgg aggttcttgc gcttcgcgag ctccgcgagg acgccctcct
     2821 cgaagtcggg ggccgcgacg acctcgcagt aggcggaggc gacgaggcgg gcggtatcga
     2881 ggtcgagggc gcggttcacg acgacggcgc cgccgaaggc cgcgacgcgg tcggcgaggt
     2941 aggcgcggcc gtaggcctcg gcgagggtcg ccgccctggc gacgcccgag gggttgttgt
     3001 gcttcatgat ggcgcaggcc gggcccgcgg agaggtagcg gaggatgttc agggccgcgt
     3061 cgacgtcggt gaggttggtc ttcccggggt gcttcccgaa ctggaggagc tcggccgcgg
     3121 cgacgaggcc gcccggcgcg ggcaggcctc cttcggagcc ctcgcccagg aactccgcgc
     3181 cgcccagggc caggttgccg cggacgggcc ggtagagggc cgcgccctgg tcggggttct
     3241 cgccgtagcg gaggcccttg cgctccccct cgacctccca ggtgagcttc tcgtaggaaa
     3301 gggccgtgcg cctgcccgtc tcgtcgtaaa aggcgatctc catccgcggg gggaagcggt
     3361 cctccgcgaa gctccggtag gcggactgaa gcgagggctt ttccgagggg gttttcatgt
     3421 tcggagtata gcggcccggg caggccgcgg gaaggggcgg cgaggcgggc cataagctgg
     3481 ccgcagagcg tccctggcgg tcgcgcggcg cggcgcctct gtatgcgcgg cgccggcgac
     3541 gccccgctac aggccccggt agcccgcctt gagctcctcg gccgtcatag cgccaaggcg
     3601 ggaggagacg gcgcggtcga agcgggagac gcggtcgaag gccgcggccg cgagcctgcg
     3661 ccggtagtcg agggaggtgt ggccgccccg gacgcggagc tcctcaagga ggcccgcgta
     3721 ctggccgggg gaggagacgg aggcgacgcg gaggtagttc ttcgccgcgg cgcggagcat
     3781 ggcgggaccg ccgatgtcga tgcgctggcg cagggcctcg ggctccgagg aagggtcgcg
     3841 gcaggcggcc tcgaaggggt agaggtcgcc cacgacgagg tcgaaggcga cggccccggt
     3901 gcgctcgagg tcggccgcgt gggcggcgtt gcccgactcc gcgaggaggc cgaggtagat
     3961 cttccagtcc agggtcttca cgagtccgcc ctccatctcg ggctggcccg tgtactcgct
     4021 caccgccacg agcctgggct cgggggcctg ccccggcgcg gggtccaggg aggcgaggac
     4081 ctccgctacc gcggcgtagc tcccccccgt cgagtagatc tcgaggccgg gggccgcgag
     4141 ggcgaggccg cgtatcaggc gcggaaggcc gtccttgtcg tagaccgaga cgagggcccg
     4201 gcggaggggg acgaggtcct ggacctcttc gacgcggttg aaggccatgg tcggctcctt
     4261 ttcgcggggc cggggggccc cgcagcggga gcgcccggcg ccccgccctg gttcagtcct
     4321 cgcccgaggg gcggcccgag ggatactcca cgtaggcgta ggccgagtgg ttgtggatgc
     4381 tctcgaagtt ctcggcctcg acggagaagc gggggaactt gcccagggcc tccacctgcc
     4441 tgtagacctc cctgacgacg tcctccacga agcgcgggtt ctcgaaggag cgctcggtga
     4501 cgtacttttc gtcgtcccgc ttgagaagcg tgtagacgcc gctcgaggcc gagccctcca
     4561 cgaggccgac gaggtcctcg atccagaaga aggggccgag ggcgaccttc agggtgacga
     4621 cgccgcgctg gttgtgggcc ccgtaggcgc tgatcgcctt ggagcagggg
//
LOCUS       DAOOSJ010000211         4658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11580_length_4658_cov_5.314795, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000211 DAOOSJ010000000
VERSION     DAOOSJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11580_length_4658_cov_5.314795"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLG14_09910"
     CDS             complement(<1..881)
                     /locus_tag="PLG14_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOX49486.1"
                     /translation="MRDELSASRGEASRAPDASPAAAPAAACSAPSGRAPAAAPSSAR
                     PLLVALAGNPNAGKTTIFNALTGARHKVGNYPGVTVEKREGRLSHRGREIRLVDLPGV
                     YSLTAYSADEVVARDFVLEERPDLVVDVLDSTNLERHLYLCLQLLELGVPVVGALNMS
                     DEAEAKGIAVDGERLGSLLGLRFVKTARGAGMEALLDAILEAAEGRGPEPRLPSYGPE
                     LDARAADLEAAIAADPAFAARVPARWLALKLLEKDEAAERLLRGERPGGGRAGPAHAR
                     LEEAELVLGEARLWLER"
     gene            complement(881..1117)
                     /locus_tag="PLG14_09915"
     CDS             complement(881..1117)
                     /locus_tag="PLG14_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOX49487.1"
                     /translation="MRLSELPAGGCFRVERILLAREVGKRLADMGFNEGRRGVVVRRQ
                     LSGGPLQVGILGGSVLIRRSEAEGIEVGVLEGRC"
     gene            1437..2717
                     /locus_tag="PLG14_09920"
     CDS             1437..2717
                     /locus_tag="PLG14_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX49488.1"
                     /translation="MERYDVVIVGTGPAGLGAAFELLGRKPGLSVLLLDKNEYSSGGL
                     RNDCKMNFSWPIGFPEYCWDEAEAGAYLARVEAFLDPNILEKKNLERYARRAEKIGVR
                     LLEIRQSHLGTDGGLELIKRLVAGLRDRGASVSLGEKALSLDAGKKTLATDRRELSYG
                     DLVVAPGRGGFEFLQGLMHEAGVPYTDNVVDVGVRIETREEHYPIVKDYYDPKFLFPK
                     KTRTFCTNSRAAHVVQEKYEAPDGGSWYSVNGHAWSASRPSNGLANFAMLKTVTLTEP
                     LASGQAYARMLGIQASLLGGGGPIMQRVGDFRLGKRSFRSSFSGDLYDFEPTLPSATP
                     GDIGLCAPAKVLRALWNAMKLLDTVVPGLLHPSTIMYYPEIKLYANRPAFVDRHFRAA
                     PGLYLIGDGAGTSRGITAAWASGLRAADGILAAK"
     gene            complement(2741..3202)
                     /locus_tag="PLG14_09925"
     CDS             complement(2741..3202)
                     /locus_tag="PLG14_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49489.1"
                     /translation="MKNTLEELVSLLPELDEEGLVFLLDQARLRRYALEVERRGLEAA
                     KEARGSLRLSGSSRTGLRMDRSADGSTYHIVSGRTWKMFTAEETAALVRIARREAPDR
                     EAASGLHSWLEAERRDFLVDLDIEGADSPRLLELLSLIRDTFPAKLPRKSF"
     gene            complement(3462..>4658)
                     /locus_tag="PLG14_09930"
     CDS             complement(3462..>4658)
                     /locus_tag="PLG14_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49490.1"
                     /translation="RSLADYQAGLKASGKSFSGELSRRYAERWTGGPLAAALAVRTTL
                     VGALHEYLGGKGLLNIERVSLSPVTDPLAHDVESTPVIGYKGVPYRTTHSMIYSKMLA
                     CFNPAIEGIFVDSPNIRLELPDGEGRQRGKYLADFSQMDVEVRRKGRIGEAMYREEPE
                     TVSAILVLERDRALDFFEGMVVHAVSRLLERNAADLASLGVALDVPARPFPRFAKDEA
                     VRRHGEAGLEKALGREAGTQFFWVLGLFRENYDLVYPFFPGDARRGVPSERIYNYDLC
                     AAARRADGGLGEAFEVLSGGLREWLYPAIVARLLDNGVIPEPPRFSPEGDLENIEAME
                     GYGPFLAAARLSGPDGLPLFPSTFGGGLGIERFLYATLRGPVLRRIEDLTYFGKNPDS
                     DDVYLF"
BASE COUNT          707 a         1671 c         1613 g          667 t
ORIGIN      
        1 tgccgctcca gccagagcct cgcctctccc aggaccagtt ccgcctcctc gaggcgggcg
       61 tgggcaggcc cggccctgcc gccgcccggc cgctcgcccc ggaggagcct ctcggccgcc
      121 tcgtccttct cgaggagctt gagcgcgagc cagcgggccg gcacgcgggc cgcgaaggcc
      181 ggatcggccg cgatggccgc ctccaggtcc gccgcgcggg cgtcgagctc ggggccgtag
      241 gaggggagcc gcggctcggg cccccggccc tccgccgcct ccaggatcgc gtccaggagg
      301 gcctccatcc cggcgccgcg ggccgtcttc acgaagcgca ggccgaggag ggagccgagc
      361 ctctccccgt cgacggcgat gcccttggcc tccgcctcgt cgctcatgtt gagggccccc
      421 acgacgggca cgccgagctc gaggagctgg aggcagagat agaggtggcg ctcgaggttc
      481 gtcgagtcca ggacgtcgac gaccaggtcg ggcctctctt ccaggacgaa gtcgcgggcg
      541 acgacctcgt ccgccgagta ggcggtgagg ctgtagacgc cgggcaagtc gacgaggcgg
      601 atctcgcggc cccggtggct gagccgcccc tcgcgcttct ccacggtgac gccggggtag
      661 ttcccgacct tgtggcgcgc cccggtgagg gcgttgaaga tggtcgtctt tcccgcgttc
      721 gggttcccgg ccagggcgac gaggaggggc cgcgccgagg agggagcggc ggcgggcgct
      781 cggccagacg gcgcggagca agccgccgcg ggtgcggcgg cgggcgaagc gtcgggcgcc
      841 cttgacgcct cgccgcggga ggcggagagc tcgtcccgca tcagcagcgg ccctccagga
      901 cgccgacctc gatgccctcg gcctcggatc tcctgatgag gaccgagccg cccaggatcc
      961 ccacctgcag ggggccgcct gagagctgcc gccggacgac gacgcctcgt cggccctcgt
     1021 tgaagcccat gtcggcgagg cgctttccga cctcgcgcgc aagcagtatc cgctcgaccc
     1081 tgaaacagcc gccggcgggg agctctgaga gacgcactgg aacctccatg gacgttcgcg
     1141 ggattagtta gctcttgcta acatttaccc gttacaaaaa gcggctcgac gccgcttgtt
     1201 agttgaggct aactcaatct ctccaaatag tcaagcacgg gctggcatcg gcctgcaggc
     1261 gcgcccatag tcgcgccaga gtcgcgccag agtcgcgcca tagtcgcgcc atagtcgcgc
     1321 cataggcgcg tcctcgggta gccacggccc gcaccgggcg gcggcgccag cgcctcgaag
     1381 agagccgaaa agactccccc ttccgcccct tcggcccttc ggcgtacaat cgcggcatgg
     1441 aaagatacga cgtcgtcatc gtcggcaccg gccccgccgg cctcggcgcc gccttcgagc
     1501 tcctcggcag gaagcccggc ctctccgtcc tcctcctcga caagaacgag tactcctcgg
     1561 gcggcctccg gaacgactgc aagatgaact tcagctggcc catcggcttc ccggagtact
     1621 gctgggacga ggccgaggcc ggcgcctacc tcgcgcgcgt ggaggccttc ctcgacccga
     1681 acatcctcga gaagaagaac ctggagcgct acgccaggcg ggcggagaag atcggcgtcc
     1741 gcctcctcga gatccggcag tcccacctgg gcaccgacgg cggcctcgag ctcatcaagc
     1801 gcctggtcgc gggcctccgc gaccgcggcg cctcggtctc cctgggcgag aaggccctct
     1861 ccctggacgc ggggaagaag acgctcgcga cggaccggcg ggagctctcc tacggcgacc
     1921 tcgtcgtcgc ccccggccgc ggcggcttcg agttcctcca gggcctcatg cacgaggcgg
     1981 gcgtccccta cacggacaac gtcgtggacg tgggcgtccg gatcgagacc cgcgaggagc
     2041 actatcccat cgtcaaggac tactacgacc cgaagttcct cttccccaag aagacgagga
     2101 ccttctgcac gaactcccgc gcggcccacg tcgtgcagga gaagtacgag gcgccggacg
     2161 gcggctcctg gtacagcgtg aacggccacg cctggtcggc ctcccggccc tccaacggcc
     2221 tcgcgaactt cgcgatgctc aagaccgtca ccctcacgga gcccctcgcc tccggccagg
     2281 cctacgcgag gatgctcggc atccaggcct ccctcctggg cggcggcggc ccgatcatgc
     2341 agcgggtcgg cgacttccgc ctgggcaagc gctccttccg ctcgagcttc tcgggcgacc
     2401 tctacgactt cgagccgacc ctgccctccg cgacgccggg cgacatcggc ctctgcgccc
     2461 cggccaaggt gctgcgcgcc ctctggaacg cgatgaagct cctggacacg gtcgtccccg
     2521 gcctcctcca cccgagcacc atcatgtact acccggagat caagctctac gcgaaccggc
     2581 cggccttcgt tgaccgccac ttccgcgcgg cccccggcct ctacctcatc ggcgacgggg
     2641 cgggcacgag ccgcggcatc accgccgcct gggcttccgg cttaagggcc gccgatggca
     2701 tcctcgccgc gaaataggcg gcggcaggag cgcccgcggg tcaaaagctc tttctgggga
     2761 gtttcgcggg gaaggtgtcg cggatgaggc tcaagagctc gaggaggcgc ggggagtcgg
     2821 cgccctcgat gtccaggtcg acgaggaagt cccgccgctc ggcctccagc caggaatgga
     2881 ggccggaagc cgcctcgcgg tcgggcgcct cccttcgggc tatcctgacc agcgcggccg
     2941 tctcctcggc ggtgaacatc ttccaggtcc tgcccgagac gatgtggtag gtggagccgt
     3001 cggccgagcg gtccatcctg aggcccgtcc tggacgagcc ggagagccgc aaggagccgc
     3061 gcgcctcctt ggccgcctcc aggccgcggc gctccacctc cagggcatag cgcctgaggc
     3121 gggcctggtc gagaaggaag acgagtccct cctcgtcgag ctcgggcagg agggagacaa
     3181 gctcctcaag ggtattcttc aagccggatt tggccattgc cgtccttcac gggcccgctc
     3241 tcgccggccc gtccagtaga gtatagcgcc cgccgaagga ttttgcctct ttttcgcctt
     3301 ttgggcaaaa ccggtacaat ctcgatttca gcccccggtc gggcggccag ccttgagcgg
     3361 cgcctccgcg cccaaggcca ggccgaccga ggccctgggc ggcgtctccg cgctcgccgc
     3421 ccggcgcggg acggcgccgg gcggccggat cacgacagcg ctcagaacag gtagacatcg
     3481 tccgagtcgg gattcttccc gaagtaggtg aggtcctcga tgcggcgcag gaccgggccg
     3541 cggagggtcg cgtagaggaa gcgctctatg ccgaggccgc cgccgaaggt ggaggggaaa
     3601 aggggaaggc cgtccggccc cgatagccgc gcggccgcga ggaaggggcc gtagccctcc
     3661 atcgcctcga tgttctccag gtccccctcg ggactgaagc gcgggggctc gggtatgacg
     3721 ccgttgtcga ggaggcgcgc gacgatggcc ggatagagcc actcgcgcag gccgccggag
     3781 aggacctcga aggcctcgcc gaggccgccg tccgcgcggc gcgcggccgc gcagaggtcg
     3841 tagttgtaga tccgctccga ggggacgccc cgcctcgcgt cgccgggaaa gaagggatag
     3901 acgaggtcgt agttctcgcg gaagaggccg aggacccaga agaactgggt gccggcctcg
     3961 cggcccaggg ccttctccag gcccgcctcg ccgtggcggc ggaccgcctc gtccttcgcg
     4021 aagcgtggga agggcctcgc cggcacatcg agggcgacgc ccagggacgc caggtcggcg
     4081 gcgttcctct caaggaggcg ggagacggcg tggacgacca tcccctcgaa gaagtcgagg
     4141 gccctgtccc gctccaggac gaggatggcc gacaccgtct cgggctcctc gcggtacatc
     4201 gcctcgccga tccggccctt gcgccgcacc tcgacgtcca tctgcgagaa gtcggccagg
     4261 tacttgccgc gctggcggcc ctcgccgtcg ggcagctcca ggcggatgtt cggcgagtcg
     4321 acgaagatgc cctcgatggc gggattgaag caggcgagca tcttcgagta gatcatcgag
     4381 tgcgtggtcc tatagggcac gcccttgtag ccgatgacgg gcgtcgactc gacgtcgtgc
     4441 gcgagggggt ccgtcacggg cgagagggag accctctcga tgttgaggag gcccttcccg
     4501 cccaggtact cgtggagggc gcccacgagg gtcgtgcgga cggccagggc agcggcgagg
     4561 ggccctcccg tccaacgctc cgcgtaccgc ctggaaagct cccccgagaa gctcttcccc
     4621 gaggccttga gtcccgcctg gtagtccgcc aggctcct
//
LOCUS       DAOOSJ010000212         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11617_length_4648_cov_5.284128, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000212 DAOOSJ010000000
VERSION     DAOOSJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11617_length_4648_cov_5.284128"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1482)
                     /locus_tag="PLG14_09935"
     CDS             complement(106..1482)
                     /locus_tag="PLG14_09935"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOX49491.1"
                     /translation="MKDVDAKTRPAEAGLRDVGEGRRDPIRILQDLIRFDTTNPPGNE
                     SGCVRYLASLLAEAGIEGELVGKDEGRLNLVARLPGRGEAPPLLLYGHADVVPADPAG
                     WRFPPFSGELAEGCVWGRGALDMKGAVAMMASALLEAAASGKRPAGDVILAFVADEED
                     GGSFGARHLVEERPGLFSGTRHAIGEIGGFTLRLGGRKFYPVMVAEKQRCGLRATWRG
                     PSGHGSMPARGSAAAKLGAALAALDRRRLPVHATEPARLMVEALAKGLGGAKGLLLRS
                     LLAPSLADGILGLMGDAGKFFDPVLHNTVNATVLRAGGKINVVPGEATAEFDARILPG
                     FGVEELLSELRGALAAGGAEEPEFEVLFYDEGPAAPDLGLFSLLSDILTEAVPSGLPV
                     PFMVSGVTDARFFSRLGIQTYGFTPMRLPPEIDFSRLIHGTDERVPAEALGFGARAMG
                     ELLARYRG"
     gene            complement(1479..2945)
                     /locus_tag="PLG14_09940"
     CDS             complement(1479..2945)
                     /locus_tag="PLG14_09940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOX49492.1"
                     /translation="MTRLKVFLAGLLARMVGLAMGERWRRSLGHEDDVRHMGLEEKLF
                     WLYKSMVRQVELAEPGSGLEERFAAQDLDFTPPDSFVKEASVVVSAGGDLSAMELIRP
                     DNTERLWDEVGGFLFGGAATDGAAAAAAPAAVPVIAVANLETPIRPGKPSVGVPAVCL
                     AAPALNGSAAMFDRFQSGGGFAYLATANNHSYDQGEDGVAETLDFLDARGVPHSGTAR
                     SPEERDAFPVLERGGVRVAFLSWTYCLNGRLLPEGKEHLANVARLNKAGFDAAPIAAQ
                     ARAARERGADFVVAVLHWSVEFEAYPTKAVAEAARRVAEAGVDVVLGGHPHNAQPSER
                     LSVRDPATGLEREALVVYSLGELVALNLHTKNSRLAQLVRVELSVGTEGGRRASRITR
                     FEILPIATSYRRSPRGAGTGGGAATAGGAAPGGPGDEYRVLDLRAALEALDRGENPEG
                     WSRREIRELKRLGALAARTMLPADAGRVLAPAGAWRKA"
     gene            complement(2957..3631)
                     /locus_tag="PLG14_09945"
     CDS             complement(2957..3631)
                     /locus_tag="PLG14_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49493.1"
                     /translation="METKALSPDEAYENKMHVIGRVTLPIGLLLTFLPPVILWLRFGI
                     LPPGKSLLQGVVGISSVMIPVSIVEILTFTPILGSAAMYMSYLTGNISNMKLPSAAIA
                     MESVGAVPGTKEGDIASTLAISGSVFASELIIALGILLIAPLSARLQTPALKPAFEQV
                     LPALFGAIGAYYIAKSWKLAAAPLAVALLLGLVPGVPTALSIPLCVLSSILAARFLYR
                     KGLVRA"
     gene            complement(3648..4364)
                     /locus_tag="PLG14_09950"
     CDS             complement(3648..4364)
                     /locus_tag="PLG14_09950"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HOX49494.1"
                     /translation="MDDYLGVANDPVFFACGAAIILFILGQAALFSRAAYREGRRLGM
                     ERRTLWRSFRAGAISSAVPTLAVIVALVTMVPVLGVPVPWIRLSVIGSAPYELMAAGI
                     GAKAMGVNGLGGSGYTAAVFASSVWVMCVGSVWSVLLVTFATKRLKKRVAGSLDKDPA
                     WRNVLMNAAFLGVFSIFIADPIVRGGLPLATLGSSALIMALVGLAVTKLRAEWLREFA
                     LALSMIGAMGAAALLAPLFS"
BASE COUNT          686 a         1602 c         1743 g          617 t
ORIGIN      
        1 cgcggtccgg cgggacggga ttcggccgcc gcccctgccg cggctcctct ggcggccgcc
       61 gcccttgcct cggctccgct agcggccgct gtccctatcc cgcctctagc cgcggtagcg
      121 ggcaaggagc tcgcccatcg cgcgggcgcc gaagcccagg gcctcggcgg gcacccgctc
      181 gtcggtcccg tggatgaggc gggagaagtc gatctccggc ggcaggcgca tcggcgtgaa
      241 gccgtaggtc tggatgccga gccgggagaa gaagcgggcg tcggtgacgc cggagaccat
      301 gaagggcacg ggcaggccgc tggggacggc ctccgtgagt atgtccgaga ggaggctgaa
      361 gaggcccagg tcaggggcgg cggggccctc gtcgtagaag aggacctcga actcgggctc
      421 ctcggccccg cccgcggcca gagccccgcg gagttccgac agaagttcct ccaccccgaa
      481 gccggggagg atccgcgcgt cgaattcggc cgtcgcctcg ccgggcacga cgttgatctt
      541 gcctcccgcg cgcaggaccg tcgcgttgac ggtgttgtgc agcacgggat cgaagaactt
      601 gcccgcgtct cccatgaggc cgaggatccc gtccgcgagg gagggcgcga ggagggagcg
      661 gaggaggagc cccttggcgc cgcccaggcc cttggccagg gcctcgacca tgagcctcgc
      721 gggctctgtc gcgtggacgg ggaggcgccg cctgtccagg gcggcgaggg cggcgcccag
      781 cttggccgcg gccgagcccc gcgcgggcat ggagccgtgg ccgctcgggc cgcgccaggt
      841 ggccctgagt ccgcagcgct gcttctcggc caccatgacg gggtagaatt tcctgccccc
      901 caggcggagg gtgaagccgc cgatttcgcc tattgcgtgc ctggtccccg agaacagccc
      961 gggccgctcc tccacgaggt gcctggctcc gaagcttccg ccgtcctcct cgtccgcgac
     1021 gaaggccagg atgacgtccc ccgcgggcct tttccccgag gccgccgcct cgaggagggc
     1081 cgaggccatc atcgcgacgg cgcccttcat gtccagggcg ccccggcccc agacgcagcc
     1141 ctcggcgagc tcgcccgaaa agggcgggaa gcgccatccg gccggatccg cggggacgac
     1201 gtcggcgtgg ccgtagagga ggaggggcgg ggcctcgccg cggccgggta ggcgcgcgac
     1261 caggttgagc cttccctcgt ccttgccgac gagctctcct tcgatccccg cctccgcgag
     1321 cagggaggcc aggtagcgga cgcagcccga ctcgttcccc ggcgggttcg tcgtgtcgaa
     1381 gcggatgagg tcctgcagga tccggatcgg atccctgcgg ccctcgccga catccctgag
     1441 gccggcctcc gccggcctcg tcttggcgtc cacgtccttc atgccttcct ccaggcgccc
     1501 gccggcgcga gcactcggcc cgcgtccgcg ggcagcatcg tcctcgcggc cagggcgccg
     1561 agccgcttga gctcccggat ctcccgccgc gaccagccct cggggttctc cccccggtcc
     1621 agggcctcga gggccgcgcg gaggtcgagg acccggtact cgtccccggg cccgccgggc
     1681 gccgcgccgc cggccgtagc cgcgccgccc cccgtccccg cgccgcgcgg gctcctcctg
     1741 taggaggtgg cgatcggcag gatctcgaag cgcgttatgc gcgacgccct gcgtcccccc
     1801 tccgtcccga cggagagctc cacgcggacg agctgggcga ggcgcgagtt cttcgtgtgc
     1861 aggttgagcg cgacgagctc gcccagggag tagacgacga gggcctcgcg ctccagtccc
     1921 gtcgcgggat cccgcaccga gagccgctcc gagggctggg cgttgtgggg gtgcccgccc
     1981 aagacgacgt cgacgcccgc ctcggcgacc cgcctcgccg cctcggcgac ggccttggtc
     2041 gggtaggcct cgaattcgac gctccagtgc aggacggcga cgacgaagtc ggcgccccgc
     2101 tccctcgcgg ccctggcctg cgcggcgatc ggcgcggcgt cgaagccggc cttgttgagg
     2161 cgggcgacgt tcgcgaggtg ctccttccct tcggggagga ggcggccgtt caggcagtag
     2221 gtccaggaga ggaaggcgac gcgcacgccg ccccgctcca ggacggggaa ggcgtcgcgc
     2281 tcctccgggc tccgcgccgt gccggagtgc ggcacccccc gcgcgtccag gaagtcgagg
     2341 gtctcggcga cgccatcctc gccctggtcg tagctgtggt tgttcgccgt cgcgaggtag
     2401 gcgaagccgc cgccggactg gaagcggtcg aacatcgcgg ccgagccgtt gagggccggc
     2461 gcggccaggc agacggcggg gacgccgacg gagggctttc ccggcctgat cggggtctcg
     2521 aggttcgcga ccgcgatgac cgggacggcg gccggcgcgg ccgcggccgc cgcgccgtcc
     2581 gtcgcggccc cgccgaagag gaagccgccg acctcgtccc agagccgctc cgtgttgtcg
     2641 ggccgtatca gctccatcgc ggagaggtcg ccgccggcgg agacgacgac gctggcctcc
     2701 ttgacgaagg agtcgggcgg cgtgaagtcg aggtcctgcg cggcgaagcg ctcctcgagg
     2761 cccgagccgg gctcggccag ctccacctgc ctgaccatgg acttgtagag ccagaagagc
     2821 ttctcctcca ggcccatgtg gcgcacgtcg tcctcgtgcc ccagggagcg cctccatctt
     2881 tcgcccatcg cgaggccgac catccgcgcg aggaggccgg cgaggaagac cttgagccgc
     2941 gtcatgccag cctccttcag gcccggacca ggcccttcct gtagaggaag cgggccgcga
     3001 ggatggagga gaggacgcag agggggatgg acagggccgt cgggacgccg gggacgaggc
     3061 cgaggaggag ggcgaccgcg aggggcgcgg cggcgagctt ccagctcttg gcgatgtagt
     3121 aggccccgat ggccccgaag agggcgggca ggacctgctc gaaggccggc ttgagcgccg
     3181 gcgtctggag cctggcggac agcggcgcga tgaggaggat gcccagggcg atgatgagct
     3241 cgctcgcgaa gaccgagcct gagatcgcca gggtggaggc gatgtcgccc tccttggtgc
     3301 ccgggacggc gccgaccgac tccatcgcga tggccgcgct cgggagcttc atgttggaga
     3361 tgttgcccgt gagataggac atgtacatcg cggcggagcc caggatgggc gtgaaggtga
     3421 ggatctccac gatggagacc gggatcatca cgctggagat cccgacgacg ccctgtagga
     3481 ggctctttcc cgggggcagg atcccgaagc ggagccagag gatcacgggc ggcaggaagg
     3541 tgaggaggag gccgatcggc agcgtcacgc ggccgatgac gtgcatcttg ttctcgtagg
     3601 cctcgtcggg cgaaagggct ttggtttcca cggctttctc ctttgcgtca gctgaacagg
     3661 ggggcgagga gggcggcggc gcccatcgcg ccgatcatgg agagggccag ggcgaactcc
     3721 ctgagccact ccgccctgag cttcgtcacc gcgaggccga cgagggccat gatgagggcg
     3781 ctcgagccga gggtggcgag ggggaggcct ccgcggacga tggggtcggc gatgaagatc
     3841 gagaagaccc cgaggaaggc ggcgttcatg aggacgttcc tccaggccgg gtccttgtcg
     3901 agcgaccccg cgacccgctt cttgaggcgc ttggtcgcga aggtgacgag gaggaccgac
     3961 cagaccgagc caacgcacat gacccagacg gaggaggcga agacggcggc cgtgtagccc
     4021 gagcctccca gcccgttcac gcccatcgcc ttggcgccga tgcccgcggc catgagctcg
     4081 tagggggccg agccgatcac ggagagcctg atccagggca cgggcacgcc caggacgggc
     4141 accatcgtga ccagggcgac gatgacggcc agggtgggga cggccgagga gatcgcgccg
     4201 gcccggaagg accgccagag ggtccgccgc tccatgccca ggcgcctgcc ttcgcgatag
     4261 gcggcccggc tgaagagtgc ggcctggccc aggatgaaga ggatgatcgc cgcgccgcag
     4321 gcgaagaaga ccgggtcgtt cgcgacgccg agatagtcgt ccatcgttgc tccttggcgg
     4381 cctgcctccc ggagcccggg gggcggccac gccgacttgg aaaccaatat agtttccaaa
     4441 gtttccaggc agtatcagcc cataccggag aactgtcaac cggaattcga gcgggccgag
     4501 cttgaccgcg gccgaggcgg ggggctatag tcgggccatg gcgactgacc ccgggcgcat
     4561 cgcgtccgcg aaccggaaca atcccaaccc ctccagcgcg gcggggccgg cttcgcgcgc
     4621 ctaggggaaa ctcaggggag acagcaag
//
LOCUS       DAOOSJ010000213         4574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11858_length_4574_cov_5.753928, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000213 DAOOSJ010000000
VERSION     DAOOSJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11858_length_4574_cov_5.753928"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1574)
                     /locus_tag="PLG14_09955"
     CDS             complement(237..1574)
                     /locus_tag="PLG14_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOX49495.1"
                     /translation="MTYLLIAYSLLLLGIALLSLKYNDSFENFLVAGRTQPRALIIAS
                     MLASTIGGGITIGTVSKAYAMGFAAFWFVAAGSLAHFLQGAFLSRRVRESEALTLPDL
                     AGKLGGRGVRKLVAVIIVFTWTGIAAGQFLAASKVVGTMTGLNHSWAVVASALFLLVY
                     TLIGGQKSVLRSDFIQFGILALALLGSLAWLYLAKSPAPGSIAIELFSPKFSPLDLSY
                     YLVVVAGSYFICPMMFGRVLSADSPANARKSSFVSGFGMLGFAFAITFIGLWARATGF
                     DAGKLDPLNAIVKNVLPGWLGVFMMFGILAAILSTADTVLLTAAGVLENDVLERKSLA
                     WTRAWVAIVAVIGAVIALYQTDIVDLLLRTYQGYTSGIVPALFVAIMAMGKRKAKPRL
                     LFAAILAGYLLGFGGNFLPTPTAQKLAAFAGLAVSAILALLSIRAGEGKKRLA"
     gene            complement(1738..3738)
                     /gene="ftsH"
                     /locus_tag="PLG14_09960"
     CDS             complement(1738..3738)
                     /gene="ftsH"
                     /locus_tag="PLG14_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOX49496.1"
                     /translation="MKLGERGPAQPPMGGWPFRFSLGYVVLMILAVSLFNYFVFQADD
                     TVVPYSVFKEKVRSGEIKRVDMDSNYYVGYAEAERQPVSILPGKTPGAAVVKTVPVYD
                     PEFTALLDEKRVVYSAVAREGNAVLSFVLNWILPLAFMIFVWRLVFKRLGNANSNVLA
                     FGQNKATIVAEGDVKTRFVDVAGVDEAKEELVEVVDFLKNPKKYTDIGGKIPKGVLLV
                     GPPGTGKTLLARAVAGEAAVSFFRMSGADFVEMFVGVGAARVRDLFKQAREKAPCIVF
                     IDELDAIGKSRLTGAVGGNDEREQTLNQLLVEMDGFDATSGLIILAATNRPDVLDPAL
                     LRPGRFDRQVLVDRPDLVGREAILRIHAKAVRLDGAVDLAKVARGTPGFVGADLANIV
                     NEAALLAVRAGRKKVVQTDFDAAIEKVVAGLEKKSRVMNPKEKRTIAYHETGHALAAA
                     HTPGADPVKKISIVPRGFGALGYTLQLPIEDRYVVTEEELLGQIDVLLGGRAAEEIAF
                     GRVSTGAANDLTRATDVARRMISDYGMSERFRNVALTRRGAGILGGQGEAMLSREYSE
                     ETQRYIDEEIARIVAARYERILDLLGTKRELLEKVASRLLEKETIEEAEFEALVAGKP
                     VSAAPETAAPEAAAPEAAAPEAAKASAPQAAHPEPEAGKAQA"
     gene            3906..>4574
                     /locus_tag="PLG14_09965"
     CDS             3906..>4574
                     /locus_tag="PLG14_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49497.1"
                     /translation="MVCLPRRRAFSIASKTSLFLAGFGLLVSYLAIVLVGTVSTRNLL
                     GGLSLVFGEASREIFPDRTEQSLLALQAEPDPYASRLFQTLTRAALSAKSVEGLSFQV
                     RRASEPGRWYRLFQGLGGRFVLIPLPEPDSAALERCLLPGLAHRVPAFLGKGASLPFR
                     VDLSRPGDEWFLVAEFRIVRAGLLAEARRNALPVLASLLSWILLSLLLARAFGRHLAQ
                     PVKEL"
BASE COUNT          704 a         1491 c         1647 g          732 t
ORIGIN      
        1 ttcgcagtgg tctgccaact agcaagcaag gagcgtgcca ggaagggagg aacggaggct
       61 agccgcgcct cgggagcccg aaaaacctgc gccgggcggg agccggccgc agccgcgcct
      121 cgctcgcgcc gcggcgtcgc gaaaaagaga gggcgcggat cgtcatccgc gccctctctt
      181 cccaatatcg cccggcagag gcgacggact ggggcgtgac gccccgcgcc ggcggctcag
      241 gccagccgct tcttcccctc tcccgccctg atcgagagga gggcgaggat ggcggagacc
      301 gcgaggccgg cgaaggccgc gagcttttgc gccgtgggcg tgggcaggaa gttgccgccg
      361 aagcccagga ggtagcccgc gaggatggcc gcgaagagga gcctcggctt ggccttccgc
      421 ttccccatcg ccatgatggc gacgaagagg gcggggacga tgccggaggt gtagccctgg
      481 taggtcctga ggaggaggtc gacgatgtcc gtctggtaga gggcgatgac ggcgccgatg
      541 acggccacga tggcgaccca ggcgcgggtc caggccaggg acttccgctc caggacgtcg
      601 ttctcgagga cgcccgcggc ggtgaggagg acggtgtcgg cggtggagag gatggcggcg
      661 aggatgccga acatcatgaa gacgccgagc cagcccggga ggacgttctt gacgatggcg
      721 ttcaggggat ccagcttgcc cgcgtcgaag cccgtcgccc tggcccagag gccgatgaag
      781 gtgatggcga aggcgaagcc cagcatgccg aagccgctca cgaaggagga cttccgcgcg
      841 ttggcggggc tgtcggcgga gaggacgcgg ccgaacatca tcgggcagat gaagtaggag
      901 ccggcgacga cgaccaggta gtaggagagg tcgagggggc tgaacttggg cgaaaagagc
      961 tcgatggcga tggagccggg agcgggggac ttggcgaggt agagccaggc cagggagccc
     1021 aggagggcga gggcgaggat gccgaactgg atgaagtcgc tccgcaggac tgacttctgc
     1081 ccgccgatga gggtgtagac gaggaggaag agggcggagg cgacgacggc ccagctgtgg
     1141 ttcaggcccg tcatcgttcc gaccaccttg ctggccgcga ggaactgccc ggcggctatc
     1201 cccgtccagg tgaagactat gatgacggcg acgagcttgc ggacgcccct tccgccgagc
     1261 ttgccggcta ggtcggggag ggtcagggcc tccgactcgc ggacccgcct ggagaggaag
     1321 gcgccctgga ggaagtgggc gagggagccg gcggcgacga accagaaggc ggcgaagccc
     1381 atggcgtagg ccttgctcac cgtgccgatc gtgatgccgc cgccgatggt ggaggcgagc
     1441 atggaggcga tgatgagggc cctgggctgg gtcctgccgg ctacgaggaa gttctcgaag
     1501 ctgtcgttgt acttgaggct caggagggct atgcccagga gcaacagcga gtaggcgatg
     1561 agaaggtagg tcatggcgag cccctttcga taagacgcat ggcatgaaaa ataggacact
     1621 gcattaagtg tcttacttat accgcgaaca tagacgaaaa gaaaactccg gtcaaggggc
     1681 cgccagactc aaggcggcgc ccgaccggag tcgcgggact caggcgatag gcggaggcta
     1741 ggcctgggcc ttgcccgcct cgggctcggg gtgggcggcc tggggcgctg aggccttggc
     1801 cgcctcgggc gccgccgctt caggcgcggc cgcctcgggc gcggcagtct cgggcgccgc
     1861 cgagaccggc ttgccggcca ccagcgcctc gaactccgcc tcctcgatgg tctccttttc
     1921 taggaggcgc gaggccacct tctccaggag ctcccgcttg gtcccgagga ggtcgaggat
     1981 gcgctcgtag cgcgccgcga cgatgcgggc gatctcctcg tcgatgtagc gctgggtctc
     2041 ctcgctgtat tcgcgggaga gcatggcctc gccctggccg cccaggatgc ccgcgccgcg
     2101 gcgggtgagg gccacgttcc tgaagcgctc ggacatgccg tagtcggaga tcatccgcct
     2161 ggccacgtcc gtcgcccgcg tgaggtcgtt cgcggccccc gtggagaccc gcccgaaggc
     2221 gatctcctcg gccgcccgcc ctcccaggag gacgtcgatc tggcccagga gctcctcctc
     2281 cgtgacgacg tagcggtcct cgatgggcag ctggagggtg tagcccaggg cgccgaagcc
     2341 gcggggcacg atggagatct tcttcacggg atcggcgccg ggcgtgtgcg ccgccgccag
     2401 ggcgtggccc gtctcgtggt aggcgatggt ccgcttctcc ttggggttca tcacccggct
     2461 cttcttctcc aggcccgcga cgaccttctc gatcgccgcg tcgaagtccg tctggacgac
     2521 cttcttcctg cccgccctca cggctaggag ggcggcctcg ttgacgatgt tggcgaggtc
     2581 cgcgccgacg aagcccggcg tcccgcgggc gaccttcgcg aggtcgacgg cgccgtcgag
     2641 cctcaccgcc ttcgcgtgga tgcgcaggat ggcctcgcgg cccacgaggt cgggccggtc
     2701 cacgaggacc tggcggtcga agcgcccggg ccggaggagg gccgggtcga ggacgtccgg
     2761 ccggttcgtc gcggccagga tgatgaggcc gctcgtcgcg tcgaagccgt ccatctccac
     2821 gaggagctgg ttcagggtct gctcgcgctc gtcgttgccg ccgacggcgc ccgtgagcct
     2881 gctctttccg atggcgtcca gctcgtcgat gaagacgatg cagggggcct tctccctggc
     2941 ctgcttgaag aggtcgcgga cccgggccgc gcccacgccg acgaacatct cgacgaagtc
     3001 ggcgccgctc atgcggaaga aggagaccgc cgcctcgccg gcgaccgcgc gcgcgaggag
     3061 ggtcttcccc gtgcccgggg ggccgaccag gaggacgccc ttggggatct tgccgccgat
     3121 gtcggtgtac ttcttcgggt tcttgaggaa gtcgacgacc tcgacgagct cctccttggc
     3181 ctcgtccacg cccgccacgt cgacgaagcg ggtcttcacg tccccttccg ccacgatggt
     3241 ggccttgttc tgcccgaagg ccaggacgtt ggagttggcg ttgccgaggc gcttgaagac
     3301 gaggcgccat acgaagatca tgaaggcgag ggggaggatc cagttgagga cgaaggagag
     3361 gactgcgttg ccctcgcggg cgacggccga gtagacgacc cgcttctcgt cgaggagggc
     3421 cgtgaactcg gggtcgtaga cggggacggt cttcacgacg gcggcgcccg gggtcttgcc
     3481 ggggaggatg gagacgggct gcctctcggc ctcggcgtag ccgacgtagt agttcgagtc
     3541 catgtcgacc cgcttgatct cgcccgagcg gaccttctcc ttgaagacgg agtagggcac
     3601 gacggtgtcg tcggcctgga agacgaaata gttgaagagg ctgaccgcga ggatcatgag
     3661 gacgacgtag cccagggaga atcggaacgg ccagcccccc atgggcggct gggcggggcc
     3721 gcgctccccg agcttcatgg gcagcttggg ctttttcttg ttccattcat tcttgctccg
     3781 gggcatccgg cactccttgt ccgcccttcc ccccgggcat ggctggcgcc ttggcgccgg
     3841 gcggggagag ggaatcgcta cctctaatct agtcattatt cgccggctcg gctatgtttc
     3901 atggcatggt ttgcctcccg cggcggcggg ctttcagcat cgccagcaag acgagcctct
     3961 tcctagcggg cttcggcctc ctggtctcct acctcgccat cgtcctggtc ggcaccgtct
     4021 ccaccaggaa tctcctgggc ggcctcagcc tggtcttcgg cgaggcatcc cgcgagatct
     4081 tccccgaccg gacggaacaa agcctcctcg ccctgcaggc ggagcccgat ccctacgcga
     4141 gccgcctgtt ccagacgctg acccgggcgg ccctgagcgc gaagagcgtc gagggcctct
     4201 ccttccaggt caggcgggcc tcggagccgg gcaggtggta ccgcctcttc cagggcctgg
     4261 gcgggcgctt cgtcctcatc cccctccccg agcccgactc ggccgccctg gagagatgcc
     4321 tcttgcccgg cttggcccat cgcgtgcccg ccttcctcgg caagggcgcc agcctgcctt
     4381 tccgcgtcga cctttcccgc cccggcgacg aatggttcct cgtagcggaa ttccgcatcg
     4441 tcagggccgg cctcctcgcc gaggcccggc gcaacgccct gccggtcctc gcctccctcc
     4501 tgtcctggat ccttctctcc ctcctcctgg cccgggcctt cggccgccac ctagcccagc
     4561 cggtgaagga gctt
//
LOCUS       DAOOSJ010000214         4555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_11922_length_4555_cov_5.676444, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000214 DAOOSJ010000000
VERSION     DAOOSJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11922_length_4555_cov_5.676444"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLG14_09970"
     CDS             <1..499
                     /locus_tag="PLG14_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49498.1"
                     /translation="AAPQAPAAFASMRDAQAAARPGTGHLTASRPNSGYAWSLELEDS
                     FLLGGGGLRSLGLSLGLRSLRTASGPRLSFDLGLTLRPIEAPGESEAAAASPGAAAPA
                     QAGLLSAAELSAKARLGLELPLRAAARLELEAALEESPLALLGPSQSLPGLELSAAYR
                     APIGP"
     gene            682..1317
                     /gene="pncA"
                     /locus_tag="PLG14_09975"
     CDS             682..1317
                     /gene="pncA"
                     /locus_tag="PLG14_09975"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOX49499.1"
                     /translation="MRLEVERSALLIVDVQNDFCPGGALGVPDGDAVIGPINRLSRRF
                     KRVVATQDWHPADHISFASNWKGKRPGDVVDAGGLEQALWPEHCLQGSGGAAFHPGLA
                     LEPVGLILRKGYHRDLDSYSALYENDRKTPTGLDGYLRSLGVRRLYLAGLATDYCVYY
                     SALDALALGYEVVLLEDAVRGVDLPAGRVQAVLAQMREAGVAFARARDYEE"
     gene            1323..2750
                     /locus_tag="PLG14_09980"
     CDS             1323..2750
                     /locus_tag="PLG14_09980"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOX49500.1"
                     /translation="MQSALFTDFYELTMAQGYFRDGPLRRSVFDVFYRRQPWGGGYAV
                     SAGLEPLLAALESFRFSEEDLAYLDSLKMFDRAFLDYLAAFRFKGDVWAIPEGRVVFP
                     QLPLLRVHADLIEAQLIEGLVLNHLNFQSLIATKAARVWRASGYGRVMEFGLRRAQGP
                     DGALSASRAAYLGGAEGTSNALAGRRYGIPVLGTMAHSWVMTYPSEREAFDAYAAIYP
                     DKTVYLIDTYDTIGSGLKNAIASGRALAEKGKRFGVRLDSGDIDYLSREVRKALDAEG
                     LDDAYIVVSNDLDEEIIEHLVADGAPVDLWGVGTKLVTGGEDAAFTGVYKLAAVERGG
                     RLEPTMKFSDNPEKSSNPGVKQAYRLYDANGGAMADLIALEEEDQPLPGREIELFHPS
                     GDWRRATVRPARVEPLLAKVMEGGRVSAPLPALADIRGSLRGRLEAFDGTYLRLLNPH
                     VYKVSISARLRDSKLKFISERFGNS"
     gene            3282..4043
                     /locus_tag="PLG14_09985"
     CDS             3282..4043
                     /locus_tag="PLG14_09985"
                     /inference="COORDINATES: protein motif:HMM:NF026198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOX49501.1"
                     /translation="MRLQEVFMALRIRAIPNNLKSGGKPYFAYASTSDLVESEEFIDR
                     MAAGRTTLSKTDILAVFQLAREELSGLLAEGCYVKTPLGSAYPVARGAFESPFEPFRP
                     GRRGTGHGLRFEFRLEAALEAEALAMLKWTREEPFDRLSPRPRSAEAIEEGASGAIRP
                     GELLRIRGERLGFDPDNESLGVFFQGESGEETRSGLYAYVKPSHLIVLVPPGLAGGSC
                     RLLVRTRSRGARILEGRLPQALAVESPQATPATSD"
BASE COUNT          708 a         1774 c         1395 g          678 t
ORIGIN      
        1 ggccgcgccc caagcgccgg ccgcgttcgc ttccatgcgc gacgcgcagg ccgccgcgcg
       61 ccccggcacc ggacacctca ccgcctcgcg accgaactcc ggctacgcct ggagcctcga
      121 gctcgaagac agcttcctcc tgggcggcgg cggcctgcgc tccctcggcc tcagcctggg
      181 cttaaggagc ctcaggaccg cctcgggccc gcgcctctcc ttcgacctcg gactgacgct
      241 gcggccgatc gaggcgcccg gcgagtccga ggccgcggcc gcctcgcccg gtgccgccgc
      301 ccccgcccag gcgggcctgc tttcggcggc cgaactctcc gcgaaggcgc gcctcggcct
      361 cgaactgccc ctgcgggccg cggccaggct ggaactcgag gccgctctcg aggagagccc
      421 cctcgccctc ctcgggccaa gccagagcct gcccggcctc gaactctccg ccgcctatcg
      481 cgcgcccatc ggcccctgac gcgacaccgc ctgcctcgcg gcgccggcca acgcgcgcgg
      541 gcgactacga tcgcggccgc gggaccggcc gccgcgcggc gccggccgcc gcgcgggcgc
      601 cgcccccggc cccgccccgc ttccccccgg cgcgccaaag cggtatcatg gcccaacact
      661 tgcggcgcgg aggaataggc tatgcgactc gaggtcgagc ggagcgcgct cctcatcgtg
      721 gacgtccaga acgacttctg ccccggcggg gccctgggcg tccccgacgg cgacgccgtc
      781 ataggcccca tcaaccgcct gtcgcggcgc ttcaagagag tcgtcgccac ccaggactgg
      841 caccccgccg accacatctc cttcgcctcc aactggaagg gcaagcgccc cggcgacgtc
      901 gtggacgcag gcggccttga gcaggccctc tggcccgagc attgcctcca gggctcgggc
      961 ggcgccgcct tccatccggg cctggccctc gaacccgtcg gcctcatcct caggaagggc
     1021 taccaccgcg acctcgactc ctactcggcc ctctacgaga acgaccggaa gaccccgacg
     1081 ggcctggacg gctacctccg ctccctgggc gtccgccgcc tctacctcgc gggcctcgcg
     1141 accgactact gcgtctacta cagcgccctg gacgccctgg ccctgggcta cgaggtcgtc
     1201 ctcctcgagg acgccgtccg gggcgtcgac ctgcccgcgg ggcgggtcca ggccgtcctt
     1261 gcccagatga gggaagcggg cgtcgccttc gcgcgcgccc gcgactacga ggagtgaccg
     1321 ccgtgcagag cgcccttttc accgacttct acgaactcac gatggcccag ggctacttcc
     1381 gcgacggccc cctgcgccgc tccgtcttcg acgtcttcta ccgccgccag ccctggggcg
     1441 gcggctacgc cgtctccgcc ggcctcgagc ccctcctcgc cgccctggag tccttccgct
     1501 tctcggagga ggacctcgcc tacctcgaca gcctcaagat gttcgaccgc gccttcctcg
     1561 actacctcgc ggccttccgc ttcaagggcg acgtctgggc catccccgag ggccgagtcg
     1621 tcttccccca gctccccctc ctgcgcgtcc acgccgacct catcgaggcc cagctcatcg
     1681 agggcctcgt cctgaaccac ctcaacttcc agagcctcat cgcgaccaag gccgcccgcg
     1741 tctggcgggc ctccggctac ggccgcgtca tggagttcgg cttacggcgg gcccagggcc
     1801 ccgacggagc cctctccgcg agccgcgccg cctacctggg cggggcagag ggcacctcca
     1861 acgccctcgc tggacggcgc tacggcatcc ccgtcctggg caccatggcc cactcctggg
     1921 tgatgaccta cccctccgag cgcgaggcct tcgacgccta cgccgccatc taccccgaca
     1981 agaccgtcta cctcatcgac acctacgaca ccatcgggag cggcctcaag aacgcgatcg
     2041 cctccggccg cgccctcgcg gagaagggga agcgcttcgg cgtgcgcctg gactcgggcg
     2101 acatcgacta cctctcccgc gaggtcagga aggccctgga cgccgagggc ctggacgacg
     2161 cctacatcgt cgtctccaac gacctggacg aggagatcat cgagcacctg gtcgccgacg
     2221 gcgcccccgt cgacctctgg ggcgtcggca ccaagctcgt cacgggcggc gaggacgcgg
     2281 ccttcaccgg cgtctacaag ctcgccgccg tggagcgcgg cggccgcctg gagcccacga
     2341 tgaagttctc cgacaacccg gagaagtcct ccaaccccgg cgtcaagcag gcctaccgcc
     2401 tctacgacgc gaacggcggc gcgatggccg acctcatcgc cctggaggag gaggaccaac
     2461 cgctgcccgg ccgcgagatc gagctcttcc acccctccgg cgactggcgc cgcgcgaccg
     2521 tccgccccgc ccgcgtcgag cccctccttg cgaaggtcat ggagggcggc cgcgtctctg
     2581 cccccctgcc cgccctggcc gacatccgcg gctccctgcg cggccgcctc gaggccttcg
     2641 acggcaccta cctccgcctc ctcaatcccc acgtctacaa ggtctccatc tccgcgcgcc
     2701 tgcgcgactc caagctcaag ttcatctccg agcgcttcgg caactcctga accacctagc
     2761 ctcaaaagat ccaaaggggg agcgcccggc ttagcggccg ggcgcctcct ttgagccgag
     2821 cggcgccgcg gcggaggcgg ctccccctcg ctccgaaagt cgcgctacgc gcgacgtttc
     2881 tccgctaccc ccactcttgc agcggagtcg cgggatgggg gcagatcccc catgccccct
     2941 cgcggcgctc gccggctcgc gccgcgcccc ctcggccgag cctccagacg ctacgctagg
     3001 ccttcggctc ggcgcgcgat gcgcgcctcc ggtcgccttc ggtaccccca ctcttgcctg
     3061 gaccggaggc ttctcggggc cgcccagggc ggagagggcc gccgcttagg ccggggaatc
     3121 gccgcgcgcg gcttggggaa ggataccgcc caagccggga agggagtttt cccaagccgc
     3181 ggccggcgcg gcgccagccc gcggacgcct cgctccgggg cagggcggaa agcctttact
     3241 tattgcaaag caaggctttg tcgatactct aaccatcgta gatgcgcttg caggaggtct
     3301 tcatggcctt gcggatacgc gcgataccga acaacctcaa atcgggcggc aagccctact
     3361 tcgcctacgc ctccaccagc gacctcgtcg agagcgagga gttcatcgac cgcatggccg
     3421 ccgggaggac caccctctcc aagaccgaca tcctcgccgt cttccagctc gcgagggagg
     3481 agctctccgg cctcctcgcc gagggctgct acgtgaagac ccccttggga tcggcctatc
     3541 cggtcgcccg cggcgccttc gaaagcccct ttgagccctt caggccgggg agacggggca
     3601 cgggccacgg cctccgcttc gagttccgcc ttgaggccgc cctggaggcg gaggccctgg
     3661 ccatgctcaa gtggaccagg gaggagccct ttgaccgcct atccccccgc ccgcggtcgg
     3721 ccgaggccat cgaggaaggc gcctccggcg cgatccgccc gggagaactc ctcaggatcc
     3781 gcggggagcg cctcggcttc gaccccgaca atgagagcct cggcgtcttc ttccagggcg
     3841 aatcaggcga ggagaccagg tccggcctct acgcctacgt gaaaccctcc cacctcatcg
     3901 tcctcgttcc gccagggctc gcgggcggaa gctgccggct tctcgtaagg acccgctcgc
     3961 gaggcgcccg aatcctcgag gggcggcttc cccaggccct cgccgtcgag agcccccagg
     4021 cgacaccagc gacctccgac tagggcgctc cgcggcggcg ttccgggcaa gacaagacgc
     4081 ttattggttt gcgccatacg caaaggagat atatactgtc gagcgatagc ggacggacca
     4141 cggcagtcac tctgttacgg cgaggaaagc gccggcccgc ccttcatccc tgcccggcga
     4201 aagccgcgaa gcacataaaa ggcccaacca tgccctcatg gcattcataa acaggagcga
     4261 ttcgatcata aaacaggatt ccgcagcgcc ttcccataag agtgtacttc ctctactccc
     4321 aagcccctcc cgcaacctgt cggcggcata atagatttta cggaacagcc taagctgttt
     4381 agtctggatg agcggataaa acggattgtg cggacgggat ttgagcgccg caatttgcag
     4441 ggagctttct tagacggttg agccgagtta tagccgagat acgtggaacg gcagaataat
     4501 tgttaggcag actaattcgt gttaaatact gaaacatgta tttgaaagga agtta
//
LOCUS       DAOOSJ010000215         4531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12011_length_4531_cov_6.762511, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000215 DAOOSJ010000000
VERSION     DAOOSJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12011_length_4531_cov_6.762511"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..988)
                     /locus_tag="PLG14_09990"
     CDS             complement(14..988)
                     /locus_tag="PLG14_09990"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX49502.1"
                     /translation="MDTGEDFYPDYREGRLDPEVAAFLRANAGPPGGAAPLRSLSPAQ
                     VRERSRLSPFLVIRDEVARKTDILVPAAGRPLRLRVYEPRGSGPFPVLLYFHGGGFVF
                     GGLDEADHLCAAFAARTPCLVVSVDYRLSPESRFPAALEDAEEALRWTCGQAAGHGGD
                     PGRIAVAGESAGANLAAVLCRLARGRGGPRLAFQLLLCPWTELSSSKSGSRGLFGRGP
                     WLSLANLDYYRDQYLGNEALAADPRVSPLRAEDLGGLPPAHVVTAEFDPLRDEGEAYA
                     RRLAEAGCRVTTRRYAGMIHSFFVLNAALGGADGAISDFAAALKEGLA"
     gene            1169..2191
                     /locus_tag="PLG14_09995"
     CDS             1169..2191
                     /locus_tag="PLG14_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOX49503.1"
                     /translation="MNRLDRVMGILLLLRGGRAVPARELAERFEVTARTIYRDIELLS
                     GLGVPVEAEMGSRGGFRLREGYFLPPVTLDPEEAVSLLLGLLLARRLRIAPFPQAAEF
                     AERKLLAALPEETRLLAARAAGLIGFESTPPDLLHPERDDPITSPARSPEEEALVVGR
                     FLRAALFRSRARLRYRSPYRAEEESLEVEPLGVVWDRDRWYLVGDAEVRPGEPRMWRS
                     DRVLEIGPAPSMRPTRGGFEAGDLLGRKWLGLAMERWRAEHPVRMALGPAQAALLKAD
                     WYYGHALFEPLADGRTVMTWGEAEPEPVLALARWLGPGAELLEPEGWRQRLAAELEAL
                     AAAYRR"
     gene            complement(2188..3102)
                     /locus_tag="PLG14_10000"
     CDS             complement(2188..3102)
                     /locus_tag="PLG14_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49504.1"
                     /translation="MNPFIGLVDSGRVADEAALKRAFRLLAKRVHPDLTGSHAPAPAG
                     ADPAESAPTAAGSAASGGAAHQGAAHQGAAHQGAAHQGAAPADPLRASRAFVALRAEY
                     EEARAYLAARAAGRAGGADSGGCAGGGGGGGGAGNGRAGADRSSAADKGPAPRAARWD
                     RRAFYESLEDLLGRGFPRRPGAAWPGRAYDASREKVLAYLAGRDQVYPEDSSREAFLA
                     FEAGYAALARGGGGWLSVDNDAGRSVYFLLADVLSYHELGFAHVRRIARSIHPDAERL
                     ARARGELRPLAFLSLLVADLEKGPAIAD"
     gene            3214..4530
                     /locus_tag="PLG14_10005"
     CDS             3214..4530
                     /locus_tag="PLG14_10005"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOX49505.1"
                     /translation="MIATETYSNYLAILKSELVPALGCTEPIAVAYAAAKARVALGAM
                     PERAELRCSGNIIKNVKGVTVPNTGGLKGVAAAAVAGVVGGDAEKGLEVLAGMGPAEI
                     EETGRLLAAGFCSVRLAEGVEGLFIEARVFAGKDSAEVVIEGTHTNVSRVSRNGELLA
                     GAAPGPGPGPAATCGDASGPPPAAGKGDRKSLNVRDILEFARAVRLCDVGELIAGQVR
                     MNSAISDEGLSRHYGVDVGKTLLKCFGGDVKTRAKARAAAGSDARMSGCAMPVVINSG
                     SGNQGMTASLPVIEYAKEFGASEERLCRALVLSNLVAIHQKTTIGRLSAYCGAVSAAC
                     GAGAGITYLSGGGYEEISRTIINTIANVGGMVCDGAKPSCAAKIATAVDAAIMAHHLS
                     AEGKVFGEGEGLVMGGVEGTIASVGRMGGVGMRATDLEVLRIMTGS"
BASE COUNT          607 a         1626 c         1715 g          583 t
ORIGIN      
        1 ccctcggtct ttctcatgcg agtccctcct tgagcgcggc ggcgaaatcg gagatcgccc
       61 cgtcggcgcc gccgagcgcc gcgttgagga cgaagaagga atggatcatg cccgcgtagc
      121 gcctcgtcgt cacgcggcag cccgcctccg cgagccgacg cgcgtaggcc tctccctcgt
      181 cgcgaagggg atcgaactca gccgtgacga cgtgggcggg gggcaggccg cccaggtcct
      241 cggcccgcag gggcgagacg cgcgggtcgg cggcgagggc ctcgttcccg aggtactggt
      301 cgcggtagta gtcgaggttg gcgagggaga gccaggggcc gcggccgaag aggccgcggg
      361 agccagactt ggagctcgag agctcggtcc aggggcagag gaggagctgg aaggcgaggc
      421 gggggccgcc gcggccgcgg gcgaggcggc agaggacggc cgcgaggttg gccccggcgc
      481 tctctcccgc gacggcgatc cgcccgggat cgccgccgtg tccggcggcc tgcccgcagg
      541 tccagcggag cgcctcctcc gcgtcctcga gggcggccgg gaaacggctc tcgggcgaga
      601 ggcggtagtc gacggagacg acgaggcagg gcgtcctggc ggcgaaggcg gcgcagaggt
      661 ggtcggcctc gtccaggccg ccgaagacga agccgccgcc gtggaagtac agcaggacag
      721 ggaagggccc cgagccgcgg ggctcgtaga cccggagcct gagcgggcgc ccggccgcgg
      781 ggaccaggat gtcggtcttc ctcgcgacct cgtcacggat gacgaggaag ggcgagaggc
      841 ggctccgctc ccgtacttgc gccgggctca gcgatcggag gggagccgcc ccgccgggcg
      901 gccccgcgtt ggcgcggagg aaggcggcga cctcggggtc gagcctccct tcgcggtagt
      961 ccggatagaa atcctcgcca gtgtccatgg cttcgcctcc gtccagtcct ggccctaagg
     1021 ccggagatgt catatattgg gagcggttgc ccgcacaggc ttggccgctc ccggcttcgg
     1081 cgcgcccatg agggctatct tcggcgcctg cgagccgagt ctacgccgcg cctgcgacag
     1141 ggcgtgtcat acatcgggga ggcgcgcgat gaatcggctc gacagggtca tgggcatcct
     1201 ccttctcctg cgcggcggac gcgcggtccc ggcgcgggag ctcgcggagc gcttcgaggt
     1261 cacggcgagg accatctacc gcgacatcga gctgctctcc ggcctcggcg tccccgtcga
     1321 ggcggagatg ggctcgcggg gcggcttcag gctccgcgag ggctacttcc tcccgcccgt
     1381 caccctcgac cccgaggagg cggtctccct cctgctcgga ctcctccttg cgaggaggct
     1441 ccgcatcgcg cccttccccc aggcggccga gttcgcggag cggaagctcc tcgccgccct
     1501 gcccgaggag acccgcctcc ttgccgcgcg cgccgccggc ctcatcggct tcgagtccac
     1561 gccgcccgac ctcctccacc cggagcgcga cgaccccatc acctcgccgg cgcgctctcc
     1621 cgaggaggag gccctggtcg tgggccgctt cctccgcgcc gccctcttcc gctcccgcgc
     1681 gaggctccgc taccgctcgc cctaccgggc cgaggaggag agcctcgagg tcgagcccct
     1741 cggcgtcgtc tgggaccgcg accgctggta cctggtcggc gacgccgagg tcaggcccgg
     1801 cgagccgcgc atgtggcgct cggaccgcgt cctggagatc gggccggctc cctcgatgcg
     1861 cccgaccagg ggcggcttcg aggccgggga tctcctcggg aggaagtggc tcggcctcgc
     1921 gatggagcgc tggagggcgg agcatcccgt ccgcatggcc ctgggcccgg cccaggccgc
     1981 cctcctcaag gccgactggt actacggcca cgccctcttc gagccgctgg ccgacgggcg
     2041 gaccgtcatg acctggggcg aggccgagcc cgagccggtg ctggccctcg cgcgctggct
     2101 cggccccggc gccgagctcc tcgagcccga gggctggagg caaaggctcg ccgccgagct
     2161 cgaggccctg gccgcggcct accggcgcta gtcggcgatc gccggcccct tctcgaggtc
     2221 ggcgacgagg agggagagga aggcgagggg gcggagctcg ccccgcgccc tcgcgaggcg
     2281 ctcggcgtcg ggatggatgg agcgggcgat gcgcctgacg tgggcgaagc cgagctcgtg
     2341 gtaggagagg acgtcggcga ggagaaagta gacggacctc cccgcgtcgt tgtccacgga
     2401 gagccagcct ccgcctcccc tggccagggc ggcgtagccc gcctcgaagg cgaggaaggc
     2461 ctcgcgggag gagtcctccg gatagacctg gtcgcggccg gcgaggtagg cgaggacctt
     2521 ctcccgcgag gcgtcgtagg ccctgcccgg ccaggccgcg ccgggccgcc tgggaaagcc
     2581 ccggcccagg aggtcctcga ggctctcgta gaaggcccgc ctgtcccagc gggcggcgcg
     2641 gggagcgggg cctttatccg ccgcggagct tcggtccgcg cccgctcggc cgtttccggc
     2701 gccgcccccg cccccgcccc cgcccgcgca gccgccagag tcggcgccgc ccgcgcgccc
     2761 ggcggccctc gcggcgaggt aggcgcgggc ctcctcgtac tcggccctga gggcgacgaa
     2821 ggcccgtgag gcgcgcaaag ggtcggcggg tgcggcgcct tgatgcgcgg cgccttgatg
     2881 cgcggcgcct tgatgcgcag cgccttgatg cgcggcgcct cccgaggcgg cgctcccggc
     2941 cgccgtcggc gcggactccg ccgggtcggc gccggcggga gcgggcgcgt ggctccccgt
     3001 gaggtccggg tggacccgct tggcgagcag cctgaaggcg cgcttgagcg ccgcctcgtc
     3061 cgcgaccctg cccgagtcga cgaggccgat gaaggggttc acggccgtta ctcctcgaag
     3121 aaagcttccc ggggccagta tagcctcccg cgcgcgcggg cgcgagcggc ctcccgctta
     3181 cggaaacggg gtaatgggcg tatactcgga accatgatcg cgaccgagac ctactcgaac
     3241 tacctcgcca tcctgaagag cgagctcgtc cctgccctgg gctgcaccga gcccatagcc
     3301 gtggcctacg ccgccgcgaa ggcccgggtc gccctgggcg ccatgcccga gcgggccgag
     3361 ctccgctgct cgggcaacat catcaagaac gtgaagggcg tcacggtccc caacaccggc
     3421 ggactcaagg gcgtggccgc cgcggccgtc gcgggcgtcg tgggcggcga cgcggagaag
     3481 ggcctcgagg tcctcgcggg catgggcccc gccgagatcg aggagacggg gcgcctcctc
     3541 gcggcgggct tctgctcggt gcgcctggcc gagggcgtcg agggcctctt catcgaggcc
     3601 agggtcttcg ccgggaagga cagcgcggag gtcgtcatcg aggggaccca cacgaacgtg
     3661 agccgcgtct cccggaacgg cgagctcctc gccggggccg ctcccggccc ggggccgggg
     3721 ccggccgcta cctgcggcga cgcgtcggga ccgcctcccg ccgccggcaa gggcgatcgg
     3781 aaatcgctca acgtgcgcga catcctcgag ttcgcgcggg ccgtccgcct ctgcgacgtc
     3841 ggggagctca tcgccggcca ggtccggatg aactcggcca tctccgacga ggggctttcg
     3901 cggcactacg gcgtcgacgt gggcaagacc ctcctcaagt gcttcggcgg cgacgtgaag
     3961 accagggcca aggccagggc cgcggcgggc tccgacgcga ggatgagcgg ctgcgcgatg
     4021 ccggtcgtga tcaactcggg aagcgggaac cagggcatga ccgcctccct acccgtgatc
     4081 gaatacgcga aggagttcgg cgcctcggag gagcggctct gccgcgccct cgtcctctcg
     4141 aacctcgtcg cgatccacca gaagaccacg atcggccggc tctccgccta ctgcggcgcg
     4201 gtgagcgcgg cctgcggcgc gggtgcgggc atcacctacc tgtcgggcgg cggctacgag
     4261 gagatctccc gcacgatcat caacacgata gccaacgtcg gcggcatggt ctgcgacggg
     4321 gccaagccct cctgcgcggc gaagatagcc accgccgtcg acgcggccat catggcccac
     4381 cacctctcgg ccgagggcaa ggtcttcggc gagggcgagg ggctcgtgat gggcggcgtc
     4441 gaggggacca tcgcgagcgt cggccgcatg ggcggggtcg ggatgcgcgc caccgacctc
     4501 gaggtgctca ggatcatgac ggggagctag g
//
LOCUS       DAOOSJ010000216         4510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12083_length_4510_cov_7.095398, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000216 DAOOSJ010000000
VERSION     DAOOSJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_4510_cov_7.095398"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLG14_10010"
     CDS             <1..985
                     /locus_tag="PLG14_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49506.1"
                     /translation="YSTWELNYQSIFPDRFRSAPEREKAAGVGTLIGVLGIAGGAILP
                     TFVIRYGKPATYLANGLVFGAVALLAALLLWPGARETPAMIRRFLEQEKARTEAPSFA
                     KQLAAAFKERNFVAFILLFFFYQSATISMTASIHYVGDYVLGGRSTTLIFAGMLSGSL
                     AAVPIWLGLGKLVRSRQALLAAAAAALSLLCLPLFFARSYGAFVAGMFAWGLAFGGFW
                     LMMTPAMADVIDEIVVKTGKREDGVYLGFRAFAGRLAYAVQALSFWAIHAATGFAQDR
                     RSASAVLGVRLHTAIVPAVLLAVGVAVFLRVNTLDAAKAEENRRLLAERGL"
     gene            1010..3169
                     /locus_tag="PLG14_10015"
     CDS             1010..3169
                     /locus_tag="PLG14_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX49507.1"
                     /translation="MSAPVRPVRRRLALLALGLSAALAVPAASAASLDLGGSVVNVRR
                     GFDPATIASPPVPGTIDWLALAADSRRSLAVRDLLLEGEARPGPFDLGSRPPESFTIA
                     FVFEADLSLINTTGLAVKVPRAGQGWALYLNGSLVRDELRLRPDGSFAAERSLRNTII
                     PLDKRLLKPGRNVLAFHVRGDPRDERTGLPGRGSILIDGYSRLVSLDREYLDIMFIGV
                     YALFGLYHFILFAQRPKDRSNLFYGLSTLVLALFLFTRTQAATLVVPDTALLGRIDNL
                     SLFLILPAFLAFFDKALGGRVSPFSKASLAFGLALAPLSFLARPEVAHNLWEVGAAVG
                     IAYLFALVLVPKMARAAREAEPGRRLGALLSSAAGRLSAGSLVAAAAVVADILAVNSG
                     GEMLFSKYAFLFLVVGAAALLADQYVSVYRELEALSVSLERKVAERSLELESAVDRQK
                     RLGAEIERKGRALAEAADASGRDLKIAERLQRGLFPARPPEVSGWDIALDYRPASGVS
                     GDFYDFFVEGGELKGLAVGDVSGHGVGAGLIAVLARSVFARRFAALGQGALGDCLAGI
                     HAELARELGGVDEYLTCLMLRIEGGRVEYANAAHPDLLFRRAGQDLARPLVPKGREGF
                     RGPPLGKEGFEGSWTALRFAPAPGDILLAFTDCLEEARNAAGERYGRARLEASVGRAP
                     AGSARELLDAVLGDIVAFAGSEALDDDLTAVVLRRLP"
     gene            complement(3166..3489)
                     /locus_tag="PLG14_10020"
     CDS             complement(3166..3489)
                     /locus_tag="PLG14_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX49508.1"
                     /translation="MSHVRQNLVYNLKRRRAALGISQMQLAARADISSGYVGELEMGR
                     KYPAPEVLERLAEALETEPFRLLMSEEDLADSGGSDAFYAAAEIIGERLKAEIEEMKK
                     RGGGA"
     gene            complement(3588..4277)
                     /locus_tag="PLG14_10025"
     CDS             complement(3588..4277)
                     /locus_tag="PLG14_10025"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HOX49509.1"
                     /translation="MKSRYLLAAAAVFLLAALPPLFAQRSLEVSVEAGADYAHTKWFG
                     PLPVKLRPQMAIWLEKADGTYVDTLYVTKAGGKGQWKGGASIRRPEALPVWAAKRGLK
                     AKDGLFMPDASTALPDAVAGATPTAGFAKSLALPAGLEPGLYRIRLEANQSFDFNAAY
                     PEKDGNVNGQPSLVWEALIQLGQAEATARLEAAGSGSPTGADGLLSPGTKGLDSALGI
                     LGKVEARYRPR"
     gene            complement(4274..>4510)
                     /locus_tag="PLG14_10030"
     CDS             complement(4274..>4510)
                     /locus_tag="PLG14_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49510.1"
                     /translation="RYYRIHDQARSLVVEALADGSMEGPRDPDLEASVGQAALWGLVA
                     QFKAKRVHPRFWNDIDPLVERFLELFLGGKGVTE"
BASE COUNT          536 a         1730 c         1528 g          716 t
ORIGIN      
        1 ctactcgacc tgggaactca actaccagtc gatcttcccc gaccgcttcc gctcggcgcc
       61 cgagcgggag aaggcggccg gcgtcggcac cctgatcggc gtcctgggca tcgccggggg
      121 ggcgatcctc cccaccttcg tgatccgcta cgggaagccc gcgacctatc tcgcgaacgg
      181 cctcgtcttc ggggccgtcg ccctcctcgc ggccctcctc ctctggcccg gcgcccgcga
      241 gacgcccgcg atgatccggc gcttcctgga gcaggagaag gcgaggacgg aggctccctc
      301 cttcgcgaag caactcgccg cggccttcaa ggagcggaac ttcgtcgcct tcatcctcct
      361 cttcttcttc taccagtcgg cgacgatctc catgacggcg agcatccact acgtgggcga
      421 ctacgtcctc ggggggagga gcacgaccct catcttcgcg ggcatgctct caggctccct
      481 ggccgccgtg ccgatctggc tcggcctcgg gaagctcgtc aggagccggc aggccctcct
      541 cgcggcggcg gccgcggccc tctccctcct ctgcctcccg ctcttcttcg cgcggagcta
      601 cggcgccttc gtggccggca tgttcgcctg gggcctcgcc ttcggcggct tctggctcat
      661 gatgaccccc gcgatggccg acgtcatcga cgagatcgtc gtgaagaccg ggaagcgcga
      721 ggacggcgtc tacctgggct tccgggcctt cgcgggccgg ctggcctacg cggtccaggc
      781 cctgtccttc tgggccatcc acgcggcgac cggcttcgcc caggacaggc gctcggcgag
      841 cgccgtcctg ggcgtgcgcc tccacaccgc catcgtgccc gccgtcctcc tcgcggtcgg
      901 cgtcgccgtc ttcctcaggg tcaacacgct cgacgcggcg aaggcggagg agaaccgccg
      961 cctcctcgcc gagcgcgggc tctagggccg cgggaaggag ctgcagccct tgagcgcccc
     1021 cgtccgtccc gtccgccgcc gcctcgccct cctcgccctg ggcctgtccg ccgccctggc
     1081 cgtccccgcg gcctccgccg cctccctgga cctcggcggc tcggtcgtca acgtcaggcg
     1141 gggcttcgat cccgcgacga tcgcctcgcc gcccgtgccc ggcaccatcg actggctcgc
     1201 cctcgcggcg gactcccggc gcagcctcgc ggtgcgcgac ctcctcctcg agggcgaggc
     1261 ccggccgggg cccttcgacc tgggctcccg cccgcccgag tccttcacga tcgccttcgt
     1321 cttcgaggcc gacctctccc tcatcaacac gacgggcctg gccgtgaagg tgccccgcgc
     1381 cggccagggc tgggccctct acctcaacgg ctccctggtg cgcgacgagc tcaggctcag
     1441 gcccgacggg agcttcgcgg ccgagcggag cctccgcaac acgatcatcc ccctggacaa
     1501 gcgcctcctc aagcccggcc gcaacgtcct cgccttccac gtccgcggcg acccccgcga
     1561 cgagcgtacg ggcctgcccg gcaggggctc gatcctcatc gacggctact cgcgcctcgt
     1621 ctccctggac cgcgagtacc tcgacatcat gttcatcggc gtctacgccc tcttcggcct
     1681 ctaccacttc atcctcttcg cccagaggcc caaggaccgc tccaacctct tctacggcct
     1741 gtcgaccctg gtcctggccc tcttcctctt cacgcgcacc caggccgcga ccctggtcgt
     1801 cccggacacg gccctcctcg gccggatcga caacctctcc ctcttcctca tcctgcccgc
     1861 cttcctcgcc ttcttcgaca aggccctggg cgggcgggtc tcccccttct ccaaggccag
     1921 cctggccttc ggcctggccc tcgcgcccct gtccttcctg gcccggcccg aggtggcgca
     1981 caacctgtgg gaggtcgggg cggccgtcgg catcgcctac ctcttcgccc tggtcctggt
     2041 gcccaagatg gcccgggccg cccgggaggc cgagccgggc aggcgcctcg gcgccctcct
     2101 ctcctccgcg gcgggccgcc tgagcgccgg ctccctggtc gcggccgccg ccgtcgtcgc
     2161 cgacatcctg gccgtgaact cgggcgggga gatgctcttc tccaagtacg ccttcctctt
     2221 cctcgtcgtg ggcgccgccg ccctcctcgc cgaccagtac gtgtcggtct accgcgagct
     2281 ggaggccctc tccgtctccc tggagcggaa ggtcgccgag cgctccctgg agctcgagtc
     2341 ggccgtggac cggcagaagc gcctgggcgc cgagatcgag cggaagggcc gcgccctggc
     2401 cgaggccgcc gacgcctcgg gacgcgacct caagatagcc gagcgcctgc agcgcggcct
     2461 tttccccgcg cggccccccg aggtctcggg ctgggacata gccctggact atcggcccgc
     2521 ctccggcgtc tccggcgact tctacgactt cttcgtggag ggcggggagc tcaagggcct
     2581 cgcggtcgga gacgtgtcgg gccacggcgt gggggccggc ctcatcgccg tcctcgcgcg
     2641 gtccgtcttc gcccgccgct tcgcggccct gggccaggga gccctgggcg actgcctcgc
     2701 gggcatccac gcggagctcg cgcgcgagct gggcggcgtc gacgagtacc tcacctgcct
     2761 gatgctccgg atcgagggcg gccgcgtcga gtacgcgaac gccgcgcacc ccgacctcct
     2821 cttccgccgc gcgggccagg acctggcccg gcccctcgtc cccaaggggc gcgagggctt
     2881 ccgcgggccg cccctgggca aggaaggctt cgagggctcc tggacggccc tccgcttcgc
     2941 gcccgcgccg ggcgacatcc tcctcgcctt caccgattgc ctggaggagg cgaggaacgc
     3001 cgcgggggag cgctacggga gggcgaggct cgaggccagc gtgggcaggg cgcccgccgg
     3061 ctcggcgcgg gagcttttgg acgcggtgct cggcgacatc gtcgccttcg cggggagcga
     3121 ggccctggac gacgacctga cggccgtcgt cctgcggcgc ttgccctagg cgcctccgcc
     3181 ccgcttcttc atttcctcga tctcggcctt gagccgctcg ccgatgatct cggcggcggc
     3241 gtagaaggcg tcggagcccc ccgagtccgc caggtcctcc tcgctcatga ggaggcggaa
     3301 gggctccgtc tccagggcct cggcgagcct ctccaggacc tccggcgcgg ggtacttccg
     3361 gcccatctcc agctcgccca cgtagcctga ggagatgtcc gcgcgcgcgg cgagctgcat
     3421 ctgcgagatg ccgagggcgg cgcggcgccg cttcaggttg tagacgaggt tctggcggac
     3481 atggctcacc ggaccatgct aggaccggcg tggccctaaa gcaattcgcc gagggagtgc
     3541 ggcgctctct gatagagagc gccgcgttgg gcgaatcggc ggccggccta gcgggggcgg
     3601 tagcgcgcct cgaccttgcc taggatgccc agggccgaat cgaggccctt cgtgccgggg
     3661 ctcaggagcc cgtcggcgcc cgtcggggag cccgagcccg cggcctcgag ccgcgccgtc
     3721 gcctcggcct ggccgagctg gatcagggct tcccacacca gggagggctg gccgttcacg
     3781 ttgccgtcct tctcggggta ggcggcgttg aagtcgaagc tctggttcgc ctccaggcgg
     3841 atccggtaga ggccgggctc gaggcccgcg ggcagggcca ggctcttcgc gaagcccgcc
     3901 gtgggggtgg cgccggcgac ggcgtcgggc agggccgtcg aggcgtcggg catgaagagg
     3961 ccgtccttcg ccttgaggcc gcgcttggcg gcccagacgg gcagggcctc gggacggcgg
     4021 atcgacgccc cgcccttcca ctggcctttg ccgccggcct tcgtcacgta gagggtgtcg
     4081 acgtaggtcc cgtccgcctt ctcgagccag atggccatct ggggcctaag cttgacggga
     4141 agggggccga accacttcgt gtgggcgtag tctgctccgg cctccacgga gacctcgagg
     4201 ctcctctgtg cgaagagggg cgggagggcg gcgaggagga agacggcggc cgcggcgaga
     4261 agatagcgtg atttcattcg gtgactcctt taccgcccag gaagagctcc aggaagcgct
     4321 ccacgagcgg gtcgatatcg ttccagaagc gcgggtggac gcgcttcgcc ttgaactggg
     4381 ccacgaggcc ccaaagggcg gcctggccga cggaggcctc caggtcggga tccctcggac
     4441 cctccatgct gccgtcggcc agggcctcca cgacgaggct ccgggcctgg tcatggatcc
     4501 ggtagtagcg
//
LOCUS       DAOOSJ010000217         4510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12085_length_4510_cov_5.370146, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000217 DAOOSJ010000000
VERSION     DAOOSJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_4510_cov_5.370146"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="PLG14_10035"
     CDS             complement(<1..1709)
                     /locus_tag="PLG14_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49511.1"
                     /translation="MDALSPETRREIVRRVFPDLRYGGDPDLERYFDLRKAGRLAEAL
                     SVYNGPLRARYPDDRARLLLLRLYRENDPRWVELQARLELELGERLAKRVRANIDALA
                     APLEGAGLSNAWKALAAAEALMRLLPRDSGEALALLERYAGFARLLSHRERLVERAGD
                     LVREYLAMARSDAPGEYDFVARSEALEKKRREEERLRRGGEPASAGGPRADERYDFIA
                     RSEALEEGRRKEEEARARYFDLSRLSFSAADLSRIEIPEEIERKEDRVLAFCAKYWRS
                     AADAGLERLVFLYSKKFGTRHYAVFRAVKVGRLRRFTDDEILTAVSTILSSSYSYSVS
                     GDLYMQEAWRRLKAGLVEAERAEAEAREAAAESPAVVGRKGRAGPPKARPLAPAAERP
                     PVARPAARAAEPRPSGRQPAARPSVAQPAARPAAPRLADRPVLDRPAVERRTPDFASR
                     PPSFALREAAVRPQASPVRSFASAPETAPGLRTSALRSAAFEADRAASVEFLPRPSAP
                     EPRSRAYREPGDDGEPRSRTRSAAAEAAEPRSRAASAPDRKPGLRWRADEGAAPGREA
                     EPRS"
     gene            complement(1874..2734)
                     /locus_tag="PLG14_10040"
     CDS             complement(1874..2734)
                     /locus_tag="PLG14_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOX49512.1"
                     /translation="MIINHNLSAMYADRSLRVTNGSMEKGMEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLERASKNAQDGISFIQTTEGYLQESQDIVQRLRELAVQASNGVY
                     TAEDRMQIQVEVSQLVDEIDRISSHAQFNGLNLLTGRFARETGENAVTGSMWFHIGAN
                     MDQREQVYIGTMTSKALGVKNVGDDSIINFESPDGSNRAIGTLDAALKKISKQRADLG
                     AYQNRLEHAIRGIDVGAENMQAAESRIRDTDMANEMVKYTKDRILAQAGNAMLAQANQ
                     KTQQVMQLLQ"
     gene            3103..3462
                     /locus_tag="PLG14_10045"
     CDS             3103..3462
                     /locus_tag="PLG14_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOX49513.1"
                     /translation="MTVRKIVEVLDARVVAGEGDLDLEVGSACGADLMSDVMAFVKEK
                     VVLLTGLVNPQAIRTADLMDIRVVVFVRGKSPSEDMVEMARENGMILLSTKYSMFLAC
                     GRLYEAGLKSGGVREID"
     gene            3469..3930
                     /locus_tag="PLG14_10050"
     CDS             3469..3930
                     /locus_tag="PLG14_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX49514.1"
                     /translation="MRYRYLVPGDDFSMAGKASAEVKRILGQLGIDPAIVKRVSVAMY
                     EAEINMAIHAGGGVAEADISPERIVVTMADEGPGISDIDLAMTEGYSTAPEWIRELGF
                     GAGMGLPNMKRNADEFGIESEVGRGTKVTMLVRFAAAAPAAAQGAEREASP"
     gene            3927..>4510
                     /locus_tag="PLG14_10055"
     CDS             3927..>4510
                     /locus_tag="PLG14_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOX49515.1"
                     /translation="MSGDGRPDWPGEADSRSEAARLLHSVRLEPEKCVGCTTCIKKCP
                     TEAIRVRKGRARIIDERCIDCGECIRTCPHGAKKAVSDPLALIAGFRYKVALPAPSLY
                     AQFDERWSIDRILSGLLALGFDEVFEVAEAAELVTDATLALLGPGRDGAMPRPVISSA
                     CPAVVKLIQVRYPSLIPHLAPLLPPVEVAARFVK"
BASE COUNT          600 a         1543 c         1673 g          694 t
ORIGIN      
        1 gagcggggct cggcctcgcg gccaggcgcg gcgccttcgt ccgcgcgcca gcgaaggccg
       61 ggcttgcggt ccggggcgga ggcggcacgc gaccgcggct cggcggcctc ggccgcggcg
      121 gagcgcgtgc gggacctcgg ctcgccgtcg tccccgggct cgcggtacgc gcgcgaccga
      181 ggctcgggcg ccgagggcct tgggaggaat tcgacgctcg ccgcgcggtc ggcctcgaag
      241 gcggcgctcc taagagcgct ggtcctgagg ccgggcgcgg tctccggggc ggaggcgaag
      301 gagcggaccg gagaagcctg agggcgcacg gcggcctcgc gcagggcgaa gctcgggggg
      361 cgcgaggcga aatcgggcgt gcgccgctcg acggcgggcc ggtccaggac gggccggtcg
      421 gcaaggcgcg gcgcggcggg ccgagcggcg ggttgcgcga cggagggccg cgcggcaggt
      481 tgccggccgg aggggcgcgg ctccgcggcc cgcgcggcgg gtcgcgcgac aggagggcgc
      541 tcggcggcgg gagcgagggg cctggccttg ggaggcccgg cgcgtccctt cctgccgacg
      601 acggcgggcg actcggcggc ggcctcgcgg gcctccgcct cggcgcgctc ggcctccacg
      661 aggccggcct tgaggcggcg ccatgcctcc tgcatgtaga ggtcgccgga gacggagtag
      721 ctgtagctcg aggagaggat ggtggagacg gcggtgagga tctcgtcgtc ggtgaagcgc
      781 ctcaggcggc ccaccttgac cgcgcggaag accgcgtagt gcctcgtgcc gaatttcttc
      841 gagtagagga agacgaggcg ctcgaggccg gcgtcggcgg ccgacctcca gtacttggcg
      901 cagaaggcga ggacgcggtc ctccttcctc tcgatctcct cggggatctc gatccgcgaa
      961 aggtcggcgg cggagaagga caggcgcgag aggtcgaagt agcgggcccg cgcctcctcc
     1021 tccttcctcc gcccctcctc cagggcctcg ctcctcgcga tgaagtcgta tctctcgtcg
     1081 gcccgcggcc cgccggccga tgcaggctcg cccccgcgcc gcagccgctc ctcctcgcgg
     1141 cgctttttct ccagggcctc gctccgcgcg acgaagtcgt actctcccgg cgcgtcggac
     1201 ctggccatcg cgaggtactc gcgcacgagg tcgcccgcgc gctcgacgag gcgctcgcgg
     1261 tgggacaaaa ggcgcgcgaa gcccgcgtag cgctcgagga gggccagcgc ctcgcccgag
     1321 tcccggggga ggagccgcat gagggcctcc gcggcggcca gggccttcca ggcgttggag
     1381 aggcccgctc cctcgagggg ggcggcgagc gcgtcgatgt tcgcccggac gcgcttggcc
     1441 aggcgctcgc cgagctcgag ctcgaggcgg gcctgcagct cgacccagcg cgggtcgttc
     1501 tcgcggtaga ggcggaggag gaggaggcgc gcccggtcgt cgggatagcg cgcgcgcagg
     1561 gggccgttgt agacggagag ggcctcggcc aggcggccgg ccttgcggag gtcgaagtat
     1621 cgctcgaggt ccgggtcgcc gccgtaccga aggtcgggga agacgcgccg gacgatctcg
     1681 cgccgggtct cggggcttag cgcgtccatc gggggggatt atacgcggcc ttgggccgag
     1741 gcgcaacgag aggggcgcgc gcagctccct ataagaacgg cccgcccccc ttgagaagga
     1801 ggacgggccg cccgagcgcc gctcccgggg cttcccttgc gggaaggggc cggggcgccg
     1861 tttcgagggg actctactgg aggagctgca tgacctgctg ggtcttctgg ttcgcctggg
     1921 cgagcatggc gttgccggcc tgcgcgagga tgcggtcctt cgtgtacttc accatctcgt
     1981 tggccatgtc cgtgtcgcgg atgcggctct ccgccgcctg catgttctcc gctccgacgt
     2041 cgatgccgcg gatcgcgtgc tcaaggcgat tctggtaggc gccgaggtcg gcgcgctgct
     2101 tcgagatctt cttgagggcc gcgtcgaggg ttccgatggc ccggttggag ccgtcgggag
     2161 actcgaagtt gatgatggag tcgtccccga cgttcttcac gccgagggcc ttcgaagtca
     2221 tcgtgccgat gtagacctgc tcgcgctggt ccatgttggc gccgatgtgg aaccacatgg
     2281 atcccgtgac ggcgttctcg cccgtttcgc gcgcgaaacg gccggtgagg agattgagcc
     2341 cgttgaactg cgcgtggctg gagatcctgt cgatctcgtc gacgagctgg ctgacttcca
     2401 cctggatctg catcctgtcc tcggcggtgt agacgccgtt cgaggcctgg accgcgagct
     2461 cgcggaggcg ctggacgatg tcctgcgact cctggaggta gccttccgtc gtctggatga
     2521 agctgatccc gtcctgcgcg ttcttcgagg cccgctcgag gccgcggatc tgcgaccgca
     2581 tcttctcgga gacggcgagc ccggaggcgt cgtcgcccgc gcggttgatc cgcatgcccg
     2641 aagagagctt ctccattccc ttttccatgc tgccattcgt gactctgaga gaacggtcgg
     2701 cgtacatagc gctgagattg tgattgatga tcatccgata ctcctttccg aattgccgcg
     2761 cgggaatcgc tccccgcgcg cggcgtcctt gccgtcgcga aatgcgccgg tccgccagca
     2821 tccttccgcg cgagtccctt gcgcgcggcg atacaacgct cgaaaggccc tgcttcggga
     2881 cgagagccgc cgggagcttg cgcctccgtc ggccccgcgc cgccccttat cggggccctt
     2941 ccctccgagt atcggaggcg gggggcgcgg ctttagggaa aagcgcgggc ggcggcggcg
     3001 ccttcccgcc ctcggggctc gccgggctgc cgtccttttt ccctcgattc acggcttgag
     3061 gatagaggcg cgccgtgcta tcctctgcgg gctggaggcc cgatgaccgt acggaagatc
     3121 gtggaagtcc tcgacgcgcg cgtcgtggcg ggcgagggag acctggacct ggaggtgggc
     3181 tcggcctgcg gcgccgacct catgagcgac gtcatggcct tcgtcaagga gaaggtcgtc
     3241 ctcctcaccg gcctcgtgaa cccgcaggcc atccgcaccg ccgacctcat ggacatccgc
     3301 gtcgtcgtct tcgtgcgcgg caagagcccg agcgaggaca tggtggagat ggcgcgggag
     3361 aacggcatga tcctcctgtc caccaagtac tcgatgttcc tcgcctgcgg gcggctctac
     3421 gaggcgggcc tcaagtcggg cggcgtccgg gagatcgact aggcgggcat gcggtatcgc
     3481 tacctcgtcc ccggggacga tttctccatg gcgggcaagg cctccgcgga ggtgaagcgg
     3541 atcctgggcc agctcggcat cgatcccgcg atcgtgaagc gcgtctccgt cgcgatgtac
     3601 gaggcggaga tcaacatggc catccacgcg ggcggcggcg tcgccgaggc cgacatcagc
     3661 cccgagcgca tcgtcgtcac gatggccgac gagggccccg gcatctccga catcgacctc
     3721 gcgatgacgg agggctactc gacggccccc gagtggatcc gcgagctcgg cttcggcgcg
     3781 ggcatgggcc ttcccaacat gaagcgcaac gccgacgagt tcggcataga gagcgaggtg
     3841 ggccgcggca ccaaggtgac gatgctcgtc cgcttcgcgg ccgcggcgcc ggccgcggcc
     3901 cagggcgccg agcgagaggc gtcgccgtga gcggggacgg gcgccccgac tggccgggcg
     3961 aggccgacag tcgcagcgag gcggcgcgcc tcctccactc ggtccgcctc gagcccgaga
     4021 agtgcgtcgg ctgcacgacc tgcatcaaga aatgcccgac ggaggcgatc agggtcagga
     4081 agggacgcgc ccgcatcatc gacgagcgct gcatcgactg cggcgagtgc atcaggacct
     4141 gcccccacgg cgccaagaag gccgtctccg atcccttggc cctcatcgcg ggcttccgct
     4201 acaaggtggc cctgccggcg ccctccctct acgcccagtt cgacgagcgc tggtccatcg
     4261 accggatcct ctccggcctc cttgccctcg gcttcgacga ggtcttcgag gtggcggagg
     4321 cggccgagct cgtcaccgac gcgaccctgg ccctcctggg cccgggccgc gacggggcca
     4381 tgccccgccc ggtgatctcc tccgcctgcc cggccgtcgt caagctcatc caggtccgct
     4441 acccgagcct catcccccac ctggcgcccc tcctgccccc ggtggaggtc gcggcgcggt
     4501 ttgtcaagga
//
LOCUS       DAOOSJ010000218         4467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12267_length_4467_cov_5.339075, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000218 DAOOSJ010000000
VERSION     DAOOSJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12267_length_4467_cov_5.339075"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..230
                     /locus_tag="PLG14_10060"
     CDS             51..230
                     /locus_tag="PLG14_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49516.1"
                     /translation="MRLDMNPRGNGACPLCGHDASCRLKRLLSGSLEDVSDAAESGME
                     IVVYSCPYFAEKARS"
     gene            227..1297
                     /gene="prfA"
                     /locus_tag="PLG14_10065"
     CDS             227..1297
                     /gene="prfA"
                     /locus_tag="PLG14_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOX49517.1"
                     /translation="MIDKLEAMSARLRELDEAVADPGVAKDPARYREIMRERARLSVI
                     DEAYTEFKSVLAGIESSKALVRDETDPEMRELAREELRGLEARRGELDERFKELLIPR
                     DPLADKNVIVEIRAGTGGDEAALFVADLFRMYSRYCDRLGWKVEVMETSETELGGYRE
                     IVFSVSGEGAYERLRWESGVHRVQRVPSTEGSGRIHTSAVTVAVLPEMEETEIDIRPE
                     DLRVDVMRAGGPGGQCVNTTDSAVRLTHLPTGLVVHCQDEKSQIKNKAKAMRILRARL
                     YELEEGKRQAERAADRKSQIGSGDRSERIRTYNFPQNRVTDHRINLTLYKLELAMEGD
                     IGELVDALMLSAREEFMKAEET"
     gene            1294..2283
                     /locus_tag="PLG14_10070"
     CDS             1294..2283
                     /locus_tag="PLG14_10070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOX49518.1"
                     /translation="MTAGDALREGARILGAARDAEGEGSETPFLDASLLLADILGMDR
                     ARLLASGPDELGPEAEARYRAALAERASGRPVAYILGRKEFYGRSFLVDERVLVPRPD
                     TELLVEAALAAGDELAVGRGAVAGAELARGGAAGRAASGGRGEGAAAAGSGPGGLRVH
                     EACVGSGCVAVSLCLERPAWEVSASDISKDALAAAARNAAALMPADRPGGPLRLLESD
                     LLSGVQGPFDIVVANPPYVPSRETDRLLGKGWGEPRLALDGGEDGLDPYRRLVPEAAA
                     RLSPGGRLLLEAGEEQAAELRSLLEAAGFEAVRTLEDLAGRPRVTEGRKPWKS"
     gene            2271..>4467
                     /locus_tag="PLG14_10075"
     CDS             2271..>4467
                     /locus_tag="PLG14_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOX49519.1"
                     /translation="MEELREGFLASVRAVYAPADSERVERALAAAEARLAGAKRASGE
                     PAAAHDLRVAAILVELGLDADSVVAGLLHDALDPVPGAPEGSEAERAAKTVAGAAAAA
                     AARAEDRRRLEAGFGPVAASLVEGVSRLAALRAKNKTVQAAETMRKMLFAMTRDIRVI
                     LVKLADKLDNMRTLKFLPEERQKEIAAECLDIFAPLADRLGVSWMKDELEDLSLKALN
                     REAFDQIKGLVSAKKGEREVYLERVERAIREAAGAEGVEVEISARAKHFYSIYQKMRK
                     KAKGAEELYDLLGVRLITESDNDCYALLGLVHRIWKPIEGRFKDYIAMPKANGYRSLH
                     TTVMGFDGRLLEVQIRTREMHRIAEYGVASHWLYKKGSTAEAPKPEDLPIVNRLKEWG
                     DFLSQGAEYLEEIKRELLKDSIFVFTPQGDVVELPAGAGPLDFAFAIHTDVGTKCMGA
                     KADGQIVPLDGELRNTQVVEILTSPQAHPTMNWLPLAKTSKARQKIRAWLVQSGQVLA
                     IERNIVAGRHTGSAAEARAREAARELKEAGAGHEAAEAKAREAREAREAAKPRGPVEN
                     LGETTVYRRVTPGEALRNEKAGVAIGGQKNLMIRIAGCCRPVTGDAIVGYVSRGRGII
                     VHRADCKSLAAVPDFQERRIEVSWESTASLVTARFRIVARRSPDLFAEIESAMHKFQG
                     HLREGRLGERGDAVSCIRQKVRYDAGHESAGEYHRVQGEGDCPVAAGNAG"
BASE COUNT          684 a         1649 c         1582 g          552 t
ORIGIN      
        1 tgccgggctc gcccttgccg ggcggccgcc ttttgcggta gcattcccgc atgagactcg
       61 acatgaatcc ccgcggcaac ggcgcctgcc ccctctgcgg ccacgacgcg tcctgccgcc
      121 tcaagcgcct cctttcgggc tccctcgagg acgtctccga cgccgccgag tcgggcatgg
      181 agatcgtcgt ctattcctgc ccctacttcg ccgagaaggc ccgctcgtga tagacaagct
      241 cgaggccatg tcggcccggc tccgcgagct ggacgaggcc gtcgccgacc cgggcgtggc
      301 gaaggatccc gcgcgctacc gcgagatcat gcgggagcgc gcccgcctct ccgtcatcga
      361 cgaggcctac acggagttca agtccgtcct cgcgggcatc gagagctcca aggccctggt
      421 ccgcgacgag accgaccccg agatgcgcga actcgcccgc gaggagcttc gcggcctcga
      481 ggcgaggagg ggcgagctcg acgagcgctt caaggagctc ctcatccccc gggaccccct
      541 cgccgacaag aacgtcatcg tcgagatccg cgccggcacg ggcggcgacg aggcggccct
      601 cttcgtggcg gacctcttcc gcatgtactc ccgctactgc gaccgcctgg gctggaaggt
      661 cgaggtcatg gagacgagcg agaccgagct cggcggctac cgcgagatcg tcttctccgt
      721 ctccggcgag ggcgcctacg agcgcctccg ctgggagtcg ggcgtccacc gcgtccagcg
      781 cgtgccctcc acggagggct cgggccggat ccacacgagc gccgtcacgg tcgccgtcct
      841 ccccgagatg gaggagacgg agatcgacat ccgcccggag gacctccgcg tcgacgtgat
      901 gcgcgcgggc gggcccggcg gccagtgcgt caacacgacc gactcggcgg tgcgcctcac
      961 ccacctcccg acgggcctcg tcgtccactg ccaggacgag aagagccaga tcaagaacaa
     1021 ggcgaaggcc atgcgcatcc tccgggccag gctctacgag ctggaggagg ggaaacgcca
     1081 ggccgagcgc gcggcggacc gcaagagcca gatcggctcg ggcgaccgct cggagcgcat
     1141 ccgcacctac aacttcccgc agaaccgcgt gacggaccac cgcatcaacc tgacgctcta
     1201 caagctggag ctcgcgatgg agggcgacat cggggagctc gtcgacgccc tgatgctctc
     1261 cgcccgcgag gagttcatga aggcggagga aacatgaccg ccggggacgc gctccgggaa
     1321 ggggcccgga tcctgggcgc ggcgcgcgac gccgaaggcg agggatccga gacgcccttc
     1381 ctggacgcgt ccctcctcct cgccgacatc ctgggaatgg accgcgcccg cctcctcgcc
     1441 tccggccccg acgagctcgg gcccgaggcc gaggcgcgct accgcgccgc cctggccgag
     1501 cgcgcctccg ggaggcccgt cgcctacatc ctgggccgca aggaattcta cggacgctcc
     1561 ttcctcgtcg acgagcgcgt cctcgtgccc cgccccgaca ccgagctcct cgtcgaggcg
     1621 gccctggccg cgggcgacga gctcgccgtg ggccgcggag cggtcgccgg ggccgagctc
     1681 gcccgcggcg gcgcggcggg gcgcgcggcc tcgggcggcc gaggcgaagg cgccgccgcc
     1741 gcggggagcg ggccgggggg gctccgcgtc cacgaggcct gcgtcggctc gggctgcgtc
     1801 gccgtaagcc tctgcctgga gcgccctgcc tgggaggtct ccgcctccga catctcgaag
     1861 gacgccctcg ccgcggcggc gcgcaacgcg gccgcgctga tgcccgcgga caggccgggc
     1921 ggccccttgc gcctccttga gagcgacctc ctctcgggag tccagggacc tttcgatatc
     1981 gtcgtcgcca acccgcccta cgtgcccagc cgggagacgg accggctcct cgggaagggc
     2041 tggggggagc cgcgcctcgc cctggacggc ggggaggacg gcctcgatcc ctacaggcgc
     2101 ctcgtccccg aggccgcggc ccggctctct cccggcggcc gcctcctcct cgaggcgggg
     2161 gaggagcagg ccgcggagct ccgctccctc ctcgaggccg ccggcttcga ggccgtccgc
     2221 acgctcgaag acctcgcggg gaggccccgc gtcaccgaag gaagaaagcc gtggaagagc
     2281 tgagggaagg attcctcgcc tccgtccgcg cggtctacgc gccggcggac tcggagcgcg
     2341 tcgagcgcgc gctggccgcg gccgaggccc gcctggccgg ggccaagcgc gcctccgggg
     2401 agccggcggc cgcccacgac ctccgcgtcg cggccatcct cgtcgagctc ggcctggacg
     2461 ccgactccgt cgtggccggc ctcctccacg acgcgctcga tcccgtcccc ggcgcccccg
     2521 aggggagcga ggccgagcgc gcggccaaga ccgtcgccgg cgccgccgcg gcggccgcgg
     2581 cccgcgcgga ggaccgccgc cgcctcgagg cgggcttcgg ccccgtggcg gcctccctcg
     2641 tcgagggcgt ctcccgcctg gcagccctca gggccaagaa caagaccgtc caggccgcgg
     2701 agacgatgcg gaagatgctc ttcgcgatga cccgcgacat ccgcgtcatc ctcgtgaagc
     2761 tcgccgacaa gctcgacaac atgcggaccc tcaagttcct cccggaggag cggcagaagg
     2821 agatcgcggc cgagtgcctg gacatcttcg cgcccctggc cgaccgcctg ggcgtgagct
     2881 ggatgaagga cgagctcgag gacctctcgc tcaaggccct gaaccgcgag gccttcgacc
     2941 agatcaaggg cctggtctcc gcgaagaagg gcgagcgcga ggtctacctg gagcgggtcg
     3001 agcgggccat ccgcgaggcg gccggggccg agggcgtgga ggtggagatc tccgcccgcg
     3061 ccaagcactt ctactcgatc taccagaaga tgcggaagaa ggcgaagggg gcggaggagc
     3121 tctacgacct cctgggcgtc cgcctcatca cggagagcga caacgactgc tacgccctcc
     3181 tcggcctcgt gcaccggatc tggaagccca tcgaggggcg cttcaaggac tacatcgcga
     3241 tgcccaaggc taacggctac cggagcctcc acacgacggt gatgggcttc gacggccgcc
     3301 tcctcgaggt gcagatccgc acgcgcgaga tgcaccggat cgccgagtac ggcgtcgcct
     3361 cgcactggct ctacaagaag ggctcgacgg ccgaggcgcc gaagcccgag gacctgccga
     3421 tcgtcaaccg cctcaaggaa tggggcgact tcctctccca gggggcggag tacctggagg
     3481 agatcaagcg cgagctcctc aaggactcga tcttcgtctt cacgccccag ggcgacgtcg
     3541 tggagctgcc cgccggggcc ggccccctcg acttcgcctt cgcgatccac accgacgtcg
     3601 ggaccaagtg catgggcgcc aaggccgacg gccagatcgt gcccctggac ggggagctcc
     3661 ggaacaccca ggtggtggag atcctcacct cgccccaggc ccatccgacg atgaactggc
     3721 tgcccctcgc caagacctcc aaggcgaggc agaagatccg cgcctggctc gtccagtcgg
     3781 gccaggtcct cgcgatcgag cgcaacatcg tcgcgggccg ccacacgggc tcggcggcgg
     3841 aggcccgcgc ccgggaggcc gcccgcgagc tcaaggaggc cggcgcgggc cacgaggcgg
     3901 ccgaggcgaa ggcccgcgag gcccgggagg cgcgcgaggc ggcgaagccg cgcggccccg
     3961 tggagaacct gggcgagacg acggtctacc gccgcgtcac gcccggagag gccctgcgga
     4021 acgagaaggc cggcgtcgcg atcggcgggc agaagaacct catgatccgc atagccggct
     4081 gctgccgtcc cgtgacgggc gacgccatcg tcggctacgt ctcgcggggg cggggcatca
     4141 tcgtgcaccg cgccgactgc aagagcctgg ccgccgtgcc cgacttccag gagcgccgca
     4201 tcgaggtgag ctgggagtcg accgcctccc tcgtcacggc gcgcttccgc atcgtcgcga
     4261 ggcggagccc cgacctcttc gcggagatcg agtcggcgat gcacaagttc cagggccacc
     4321 tccgcgaggg gcggctcggc gagcggggcg acgccgtctc ttgtatccgc cagaaggtcc
     4381 gatacgatgc gggacatgaa tctgccggag aataccaccg ggtgcagggc gagggcgatt
     4441 gcccggtggc tgcaggaaac gcgggcg
//
LOCUS       DAOOSJ010000219         4452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12328_length_4452_cov_4.598817, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000219 DAOOSJ010000000
VERSION     DAOOSJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12328_length_4452_cov_4.598817"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLG14_10080"
     CDS             <1..1281
                     /locus_tag="PLG14_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOX49520.1"
                     /translation="FVERRRGSGTYVARRRGAGRARASAGEGEAVEPVPAWQAELADR
                     GGLAEPGLGMGTAAAPGFVDLASGLSDPALFPAEDFRRALREVLAFQGDEAFGYGDPR
                     GYLPFREAVARVLASRGVDARAEEILVTSGSQEALCLCALLARERGGPVLVETPTYAA
                     ALSLFRSLGLEVRGLDSDSGGPRLEALERALAGERPSLLYLMPNFRNPSGTSVEGPRR
                     AALVALAERACLPILEDDYVGDLRFEGRDLPALAAYGSPGATLHAGTFSKMLAPGLRV
                     GFLVARGPVYRRLLELKYSVSMAAPNLAQRALAEILSVGSYERHLGRSRRAYRARRDA
                     MLRGLAALPPGYAAERPSGGLFAWLRLPPGASASALAAAAAARGLLVYDGGRSFPDRG
                     HPLADSFLRLNFAASTEAAIGEGLARLAQAAASL"
     gene            1366..2022
                     /locus_tag="PLG14_10085"
     CDS             1366..2022
                     /locus_tag="PLG14_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOX49521.1"
                     /translation="MAVLTVSRELGSGGRTVARKAAEILGYRFADKKLLGEILGEYGL
                     VEFPREYEESRGFWDYFDTRGEAMVEMLNRAILAVAATDEVVILGRGCFALLRGYEDV
                     VNARIQAPFAERARRVAARDGYEEAAKAEDYVRKADKARRSFVEQVYGLRWGDAEPFD
                     LVVDTAKVDAETAASWLVEAVGVVEARPSEGGRRVSRIEVDPTLAEAVEEAFAKAEEA
                     "
     gene            2099..2632
                     /gene="purE"
                     /locus_tag="PLG14_10090"
     CDS             2099..2632
                     /gene="purE"
                     /locus_tag="PLG14_10090"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOX49522.1"
                     /translation="MESSKNGGPLVGIVMGSDSDLPVMAEAARILAELGVDCETTIVS
                     AHRTPARLFDYARQAAGRGLEAIVAGAGGAAHLPGMVASLTRLPVIGVPVRTKTLDGL
                     DSLLSIVQMPPGIPVATVAVDGARNAGLLAARVLALSRPALAKRLEDYAARLEAEVLS
                     KAARLENLGPEDYLAAK"
     gene            2837..3826
                     /locus_tag="PLG14_10095"
     CDS             2837..3826
                     /locus_tag="PLG14_10095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOX49523.1"
                     /translation="MEILKPSEENIRRAGEALARGLLVAIPTETVYGLGASAFDVRAV
                     ARVFEAKNRPVFDPLIVHVDSLEMVDRVATIPSPRVRALMEGLWPGPLTLILPRREAV
                     PDLVTSGLPTVAVRLPANAIARAVIAAAGLPVAAPSANPFGYLSPTLPEHVARMLGGR
                     VDFIVDGGACPIGVESTILDATKEPFTVLRPGGMPLEAIEELAGPVEVFNRSVARPTS
                     PGQLPSHYAPRTPLYLFPSGGLAEARPVGRAAALLFDPASLAALERSGRAAAFAELRV
                     LAPGGTSLEAAAALFAVLHELDGGGFDAICAERLPEAGLGRAVNDRLYKASEK"
     gene            3874..4263
                     /locus_tag="PLG14_10100"
     CDS             3874..4263
                     /locus_tag="PLG14_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49524.1"
                     /translation="MTAAVASSYVPLSYAHAAGAGAGRISLPVPASQLLYSGFEHVTG
                     VPAPEGGTALSIDRLKILDVLIDRLSSVKSEPLAAREAPSSLTSGRVDALIEQYGSEL
                     HALAAAPSLPYAQVPSIPSGSLFSLAA"
BASE COUNT          525 a         1783 c         1549 g          595 t
ORIGIN      
        1 ttcgtggagc ggcggcgggg cagcgggacc tatgtggccc gtcgccgcgg ggccgggcgc
       61 gcgcgggcgt cggcggggga gggagaggcg gtagagcccg tcccagcctg gcaggccgag
      121 ctcgccgacc ggggcggcct cgcggagccc ggcctgggca tggggacggc ggccgcgccg
      181 ggcttcgtcg acttggcctc gggcctgtcc gatcctgccc tcttcccggc cgaggacttc
      241 aggcgggccc tgagggaggt cctggccttc cagggcgacg aggccttcgg ctacggcgac
      301 ccccgcggct acctgccttt ccgtgaggcc gtcgcccggg tgctcgcgag caggggcgtc
      361 gacgcgaggg ccgaggagat cctcgtcacc tccggctcgc aggaggccct ctgcctctgc
      421 gccctcctcg cgcgcgagcg gggagggccc gtcctcgtgg agacgccgac ctacgcggcc
      481 gccctttccc tcttccgctc cctcggcctc gaggtccgcg gcctcgactc cgactcgggc
      541 gggcccaggc tcgaggccct ggagcgggcc ctggccgggg agcggccctc cctgctctac
      601 ctcatgccca atttccggaa cccttccggc acgagcgtgg aggggccgcg ccgggccgcc
      661 ctcgtggccc tggccgagcg cgcctgtctg cccatcctgg aggacgacta cgtcggcgac
      721 ctgcgcttcg agggacggga cctgcccgcc ctcgccgcct acggcagtcc cggcgcgacg
      781 ctccacgcgg gcaccttctc caagatgctc gcccccggcc tccgcgtcgg cttcctggtc
      841 gcccgcggcc ccgtctaccg ccgcctcctc gagctcaagt acagcgtctc catggccgcc
      901 cccaacctcg cccagcgggc cctggcggag atcctctccg tcgggagcta cgagcgccac
      961 ctgggccgct cgcgccgggc ctaccgcgcg cggcgcgacg cgatgctccg gggcctggcc
     1021 gcccttccgc ccggctacgc cgccgagagg ccctccggcg gcctcttcgc ctggctccgc
     1081 ctccctccgg gggcgagcgc ctccgccctc gccgccgcgg cggccgcccg gggcctcctg
     1141 gtctacgacg ggggccgctc cttccccgac cgcgggcacc ccctcgccga ctccttcctc
     1201 cgcctcaact tcgccgcctc gacggaggcg gcgatagggg aggggctcgc ccgcctggcc
     1261 caggccgccg cctccctgta gccgtcgccg ccgagccgcc aggtcagcgc cgccccctgg
     1321 actcgacccc cgcaaaggct atgctttaaa gcgaggaggc gcgccatggc ggtgctcacg
     1381 gtctctcggg agctcgggag cggcggcagg accgtcgcgc ggaaggcggc ggagatcctc
     1441 ggctaccgct tcgccgacaa gaagctcttg ggcgagatcc tcggggaata cggcctcgtc
     1501 gagttcccca gggagtacga agagtcgagg ggcttctggg actatttcga cacgcggggc
     1561 gaggcgatgg tcgagatgct caaccgggcc atcctggccg tcgcggccac cgacgaggtc
     1621 gtcatcctgg ggaggggctg cttcgccctc ctccgcggct acgaggacgt cgtcaacgcg
     1681 aggattcagg cgcccttcgc cgaaagggcc aggcgggtcg ccgcccgcga cggctacgag
     1741 gaggcggcga aggccgagga ctacgtgcgg aaggccgaca aggcccgccg gagcttcgtg
     1801 gagcaggtct acggcctgcg ctggggcgac gcggagccct tcgacctggt cgtcgacacg
     1861 gcgaaggtcg acgcggagac ggcggcctcc tggctcgtcg aggctgtcgg cgtcgtcgag
     1921 gccaggccct ccgaaggcgg ccgccgcgtc tcccggatcg aggtggaccc gaccctcgcc
     1981 gaggcggtgg aggaggcctt cgccaaggcc gaggaggcct aggaacctac gcggcggccg
     2041 cgcggcgggc cggcggcggg cctagctagg ctcgacgccc cgggctacta tgcgaggcat
     2101 ggaaagctcg aagaacggcg ggccgctcgt cggcatagtc atgggctcgg actccgacct
     2161 gcccgtcatg gcggaggcgg cgaggatcct cgccgagctc ggcgtggact gcgagaccac
     2221 gatcgtctcc gcgcaccgca cgcccgcgcg ccttttcgac tacgcgcgac aggccgcggg
     2281 gcggggcctg gaggccatcg tcgcgggggc cgggggcgcc gcccacctgc ccggcatggt
     2341 cgcgagcctg acgaggctcc ccgtgatcgg cgtgcccgtc cgcacgaaga ccctggacgg
     2401 cctcgacagc ctcctctcca tcgtccagat gccgccgggc atccccgtcg cgacggtcgc
     2461 cgtcgacggg gccaggaacg ccggcctcct cgccgcccgc gtcctggccc tctcccggcc
     2521 ggccctcgcg aagcgcctgg aggactacgc cgcccgcctg gaggccgagg tcctttcgaa
     2581 ggccgcccgc ctggagaacc tcgggcccga ggactacctg gccgcgaagt aggccgccgc
     2641 gcggtcctgg cccctcgccg cggcagcgcc cacgggccgc cgcggccgcg cggcgacgcg
     2701 gtttcgcgcc gcgggctcgg ccgcgccgcg ggctcagccg cgcccctgga ggccgcactg
     2761 ggccgccgtc cgtggagccg cggcccaagg cccggtagcc gaggcgggcc cctcgggcgt
     2821 atcatcctgt ccgaccatgg aaatcctcaa gccctccgaa gagaacatcc gccgcgccgg
     2881 cgaggccctc gcccgcggcc tcctcgtcgc cataccgacg gagaccgtct acggcctggg
     2941 cgcgagcgcc ttcgacgtcc gcgccgtcgc ccgcgtcttc gaggccaaga accggcccgt
     3001 cttcgatccc ctcatcgtcc atgtcgacag cctcgagatg gtcgaccggg tcgcgaccat
     3061 cccctcgccc agggtccgcg ccctgatgga gggcctctgg cccggccccc tgaccctcat
     3121 cctgccgcgg cgcgaggccg tgcccgacct cgtgacgagc ggcctcccga ccgtggccgt
     3181 gcgcctcccc gcgaacgcga tagcccgcgc cgtcatcgca gcggccggcc tccccgtcgc
     3241 ggcgccgagc gccaacccct tcggctacct ctcgccgacc ctgccggagc acgtggcgag
     3301 gatgctcggc gggcgcgtcg acttcatcgt cgacggaggc gcctgcccca tcggcgtcga
     3361 gtcgacgatc ctcgacgcga ccaaggagcc cttcacggtc ctgcgcccgg gcgggatgcc
     3421 cctcgaggcc atcgaggagc tcgccggccc cgtcgaggtc ttcaaccgct ccgtcgcgag
     3481 gccgacgagc ccgggccagc tccccagcca ctacgcgccc aggacgcccc tctacctctt
     3541 cccctcgggc ggcctggccg aggcgcggcc agtcggacgg gcggcggccc tcctcttcga
     3601 cccggcctcc ctcgcggccc tggagcgctc gggccgcgcc gccgccttcg cggagctccg
     3661 cgtcctcgcg cccggaggaa cctcgctgga ggccgccgcc gccctcttcg ccgtcctcca
     3721 cgagctggac ggcggcggct tcgacgcgat ctgcgccgag cgcctgcccg aggcgggcct
     3781 gggaagggcg gtcaacgacc gtctctacaa ggcctcggag aaatagctca acccgggccg
     3841 cctccgttcc gaaattcggt agtggaggca gccatgacgg cagcggtggc atcgagctat
     3901 gttcctctgt cctacgccca cgcggcgggc gcgggcgccg gaaggatcag ccttcccgtg
     3961 ccggcctcgc agctcctcta ttccggcttc gagcacgtga cgggcgtccc cgcccccgag
     4021 ggcgggacgg ccctcagcat cgaccggctc aagatcctgg acgtcctgat cgaccggctg
     4081 tcgagcgtca agtccgagcc cctggccgcg cgcgaggccc cctcgagcct cacctcgggc
     4141 agggtcgacg ccctcatcga gcagtacggc tccgagctcc acgccctggc cgcggccccc
     4201 tccctgccct acgcgcaggt cccctccatt ccctcaggca gtctcttctc cctcgccgcc
     4261 tagcgcgtcc tgcgcggccg ggcagccgcg gcgtggcagc cgcgcgcccg cccccgcgga
     4321 cttggccccg cgcggccggt cagccgcggc cctagcgccg cgaggccgcc tcgccgagca
     4381 tcccgtccac ggcggccgcc ggatccatcc cgtagtacag ctccccgtag atcgtcgccg
     4441 cctggaggat gt
//
LOCUS       DAOOSJ010000220         4435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12387_length_4435_cov_5.827854, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000220 DAOOSJ010000000
VERSION     DAOOSJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12387_length_4435_cov_5.827854"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..845)
                     /locus_tag="PLG14_10105"
     CDS             complement(201..845)
                     /locus_tag="PLG14_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOX49525.1"
                     /translation="MKACVVADAPPYLAYGEERDPEGFLSFFILPRLPSATGNAFAPG
                     VRPPEADLYVLPAELYLSLPAEARPRPAFAYGPERLMPAAFEAGCADYLREPWGPSEL
                     RARARRLLSFRFAPGSGSVELKGRDLVSGGRTERLTEAERRLFRLLLLNLGETVPCKA
                     LELALWGEERPASRAPGVHVSSIRRKLERLSPGSGKALRAHRGRGYSFEAVRCG"
     gene            complement(879..1589)
                     /locus_tag="PLG14_10110"
     CDS             complement(879..1589)
                     /locus_tag="PLG14_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOX49526.1"
                     /translation="MGIFERMKTVISSNINDLISKSENPEKMLNQLVLDMNEQMIESK
                     KAVAMAIADEKKLEREALENKRQADDWEKKAMLAVRAGRDDLAKEALVRKQEYEGYAV
                     QLSTQWQAQKESVEKLKESLRQLQNKIDEASRKKNILIARAKRAEAQDKINKTMSSIS
                     GNKSAFDTFDRMSKKVDEIEARTEAHKELEEMSSGASLEKQFAQLESSGAGADLLLEQ
                     LKAKMLTDGSGSAPQGNP"
     gene            complement(1603..1986)
                     /locus_tag="PLG14_10115"
     CDS             complement(1603..1986)
                     /locus_tag="PLG14_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOX49527.1"
                     /translation="MAGLTKIEEYLIDLGVSYEEIGASTWLVDDPEKGLLKIVVSYTE
                     PVVVIRASVMPAPSSGREAFFAELLKLNGEDLLHGAYALDGDDVVIVDTLEYADMDKS
                     EFEASLDAIGFALAQHYPLLSRFKN"
     gene            complement(2021..2572)
                     /locus_tag="PLG14_10120"
     CDS             complement(2021..2572)
                     /locus_tag="PLG14_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOX49528.1"
                     /translation="MSNNDIVPGFDDEKDDSLKIRLQKVDAVDNCLTLFLTGYIDTYN
                     SNFFQKRIGKAIESGFIRLIFNCGGLNYVSSTGIGSFTAFLKSVKPRGGDIVLLEIQP
                     KVYEVFQLLGFSQFFNIKDNIDEATGFFRQGDQGSAASVFPKIFSCPICNKKLKAARP
                     GRFRCSECKTILAIDNAGQVFLG"
     gene            complement(2818..3666)
                     /locus_tag="PLG14_10125"
     CDS             complement(2818..3666)
                     /locus_tag="PLG14_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX49529.1"
                     /translation="METPQDRGRGQAAGRRRKDRPSPLAGRRALVVGGSGGIGAALAA
                     ALAARGADLLIHGGSSRERLAAAVARAEEAGRGALGDEASGRAPAGASARPSVESLLL
                     PLERPSDLVDRLPGLGRVDILAVAFGPFVRKALHETGAADWERLALLDLALPGALASA
                     LIPAMAARGYGRILFFGGTRTDAIRAYSSNAAYAAAKTGLAVLAKSIAAEYSQAGIGA
                     FVLCPGFVDTEYLGEGQRAELRSKAPGGRLIEAEEIAEFAASLVAAEPCIASGAVVAL
                     DGGLKL"
     gene            3792..>4435
                     /locus_tag="PLG14_10130"
     CDS             3792..>4435
                     /locus_tag="PLG14_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX49530.1"
                     /translation="MSEPDLSRYVVVLCRAEEAGNVGSACRAMKTMGLTRLRLAACPA
                     FDEARVRTMAVHAADLYEAAERVPSLGEALAGTALSAGFTRRRGERRKSFSVDAETFA
                     RTAADRPGGDIALVFGNEKSGLSEEELSACSLAVHIPTSSAFPSLNLAQAVQIAVYEV
                     RRAALGGRKGSYEPAPRAAVEEAVGRMSRRLEELGFFRLNQGADLKAFLRDTAER"
BASE COUNT          668 a         1450 c         1558 g          759 t
ORIGIN      
        1 cgcaaggttt tttcccgtac agggtaaaag cctaaaaaca tcaaagacct ctagctactt
       61 tgttaattca ttgtggagta tgacaaaaac aaaattatgc acagtttttt ccgcaggctt
      121 cgggcaagac cgactaagac gctcgtcaat ttgtacggtt atttttccgc ttggggaaag
      181 gcccccagat tgtgcacgac ttatccacaa cggactgctt cgaagctgta acctcttcca
      241 cgatgggcac gtagcgcctt tccggacccg ggcgagagtc tctcgagctt gcgccggatc
      301 gaggagacgt ggactcccgg agcgcgggaa gcgggccttt cctcgcccca gagggccagc
      361 tccagggcct tgcagggcac ggtctcgccc aggttgagga ggaggagacg gaagagccgc
      421 cgctccgcct ccgtcagccg ctcggtccgc cccccggaga ccaggtcgcg tcccttgagc
      481 tcgacgctgc ccgagccggg ggcgaagcgg aaggaaagga ggcgcctggc cctggcccgg
      541 agttcggagg ggccccaggg ctcgcggagg tagtcggcgc agccggcctc gaaggcggcg
      601 ggcatgaggc gctcggggcc gtaggcgaat gcgggccggg gccgcgcctc ggccgggagg
      661 gagagataga gctcggccgg caggacatag aggtcggcct cgggaggccg gacgcccggg
      721 gcgaaggcgt tccccgtggc cgaaggcagg cgcgggagaa tgaaaaagga gaggaatccc
      781 tcgggatccc tctcctctcc gtaggcgaga tagggcggag cgtcggctac gacgcaggcc
      841 ttcactctcg tcggcccgtc gggcggcctt ggccgtccct aggggttgcc ctgcggcgcg
      901 gagcccgagc cgtcggtgag catcttggcc ttgagctgct cgaggaggag gtcggcgccc
      961 gcgcccgagg actcgagctg ggcgaactgc ttctccaggc tcgcgccgga ggacatctcc
     1021 tcgagctcct tgtgggcctc ggtcctggcc tcgatctcgt cgaccttctt ggacatgcgg
     1081 tcgaaggtgt cgaatgcgct cttgttgccc gagatgctcg acatcgtctt gttgatcttg
     1141 tcctgggcct cggcgcgctt ggcgcgggcg atgaggatgt tcttcttgcg ggaggcctcg
     1201 tcgatcttgt tctggagctg ccgcaggctc tccttgagct tctcgaccga ctccttctgg
     1261 gcctgccact gggtggagag ctggaccgcg tagccctcgt actcctgttt ccggaccagt
     1321 gcctccttgg cgaggtcgtc ccggccggcg cggaccgcga gcatggcctt cttctcccag
     1381 tcgtcggcct ggcgcttgtt ctccagcgcc tcgcgctcga gcttcttctc gtccgcgatg
     1441 gccatcgcga cggccttttt cgactcgatc atctgctcgt tcatgtccag gacgagctgg
     1501 ttcagcatct tctccgggtt ctcggacttg ctgatgaggt cgtttatatt cgacgagatg
     1561 accgtcttca tgcgctcgaa aatgcccatt ggggtttcct cctcagttct tgaagcggga
     1621 gagcagggga tagtgctggg ccagggcgaa gccgatcgcg tcgagggagg cctcgaattc
     1681 cgacttgtcc atgtccgcgt attccagggt gtcgacgatg acgacgtcgt cgccgtcgag
     1741 ggcgtaggcg ccgtggagga ggtcctcgcc attgagcttg aggagctcgg cgaagaaggc
     1801 ctcccggccg gaggaggggg cgggcatgac gctcgcccgg atgacgacga cgggctcggt
     1861 gtaggagacc acgatcttga ggaggccctt ctcaggatcg tcgacgagcc aggtggaggc
     1921 gccgatctcc tcgtaggaga cgcctaggtc gatgaggtac tcttcgatct tcgtgagtcc
     1981 ggccatgggg gacactcctt ggggcctcgc gcggcccgcg ctagccgagg aagacctggc
     2041 ccgcgttgtc gatcgcgagg atcgtcttgc actcggagca gcggaagcgg ccgggccgcg
     2101 ccgccttgag cttcttgttg cagatggggc aggagaagat cttcgggaac acggaggcgg
     2161 cggagccctg gtctccctgg cggaagaagc cggtcgcctc gtcgatgttg tccttgatgt
     2221 tgaagaactg ggagaagccg aggagctgga agacttcgta gaccttgggc tggatctcga
     2281 ggaggacgat gtcgccgccg cggggcttca cggacttgag gaaggcggtg aaggagccga
     2341 tgccggtgga ggagacgtag ttgagcccgc cgcagttgaa gatgaggcgg atgaagccgc
     2401 tctctatggc cttgccgatc ctcttctgga agaagttgga gttgtaggtg tcgatatacc
     2461 cggtaaggaa gagagtgagg cagttgtcca cggcgtcgac tttctggaga cggatcttga
     2521 ggctgtcgtc cttctcgtcg tcgaagccgg gcactatatc attattgctc atggtactcc
     2581 tcttcgcccc tcacggtact cggaatctat tgtagaagca taatatgaac aagtcaattc
     2641 gttgtcaaat tccggaaagg gcccttcgag gggcccgcga tgcccggcgg ccgcggcgcc
     2701 gccgccctcc ggcggccgct ttccggcccg tccggcgcgc ccctggaccc ggtcgccgcg
     2761 gcgcgctctc gtacatgtat cggccgattc aggcccgccc tcaagccccg ccttggccta
     2821 gagcttgagc cccccgtcca gggcgacgac cgcgcccgag gcgatgcagg gctcggcggc
     2881 gaccagggag gcggcgaatt cggctatctc ctcggcctcg atgaggcgtc cgccaggggc
     2941 cttcgacctg agctcggccc gctggccctc ccccaggtac tccgtgtcga cgaagccggg
     3001 gcagaggacg aaggcgccga tgccggcctg cgagtactcc gcggcgatgg acttcgcgag
     3061 gacggccagg cccgtcttgg ccgcggcgta ggccgcgttg gaggagtagg ccctgatcgc
     3121 gtccgtcctc gtgccgccga agaagaggat gcggccgtag cctcgggccg ccatcgcggg
     3181 gatgagtgcc gaggcgaggg cgccgggcag ggccaggtcg aggagggcga gccgctccca
     3241 gtccgcggcc ccggtctcgt ggagggcctt ccttacgaag gggccgaagg cgacggccag
     3301 gatgtcgacg cggcccaggc cgggaaggcg gtcgaccagg tcggacggcc gctcgagggg
     3361 gaggaggagg gactcgaccg agggccgggc cgacgctccg gccggggccc ggccagaggc
     3421 ctcgtccccg agggcgcccc gtccggcctc ctcggcacgg gcgacggcgg ccgcgaggcg
     3481 ctccctggag ctgcccccgt ggatgaggag gtcggctccc cgggcggcca gggctgcggc
     3541 cagggccgcc ccgatgcctc ccgagccccc gacgacgagg gcccgccttc cggcgagcgg
     3601 gctgggcctg tccttccggc gccggcccgc ggcctggccg cggccgcggt cttgcggcgt
     3661 ctccatacgg aagctactat aacggcgcct cgccgccggc cgcaagccgg cccggcgcgc
     3721 cttccttata tacgccatag atgcgcggcc aggcggcgcg cggagcggca ggtagccttc
     3781 aaggagccag agtgagcgag cccgaccttt cccgctacgt cgtcgtcctc tgccgcgccg
     3841 aggaggcggg caacgtaggc tccgcctgcc gcgccatgaa gaccatgggc ctcacgcggc
     3901 tccgcctcgc ggcctgcccg gccttcgacg aagcccgcgt ccgcacgatg gccgtgcacg
     3961 ccgccgacct ctacgaggcc gccgagcgcg tcccgagctt gggggaagcc ctggccggga
     4021 cggccctctc cgccggcttc acccggcgcc gcggcgagag gcggaagtcc ttctccgtcg
     4081 acgccgagac cttcgcgagg acggcggccg accggcccgg cggcgacatc gccctggtct
     4141 tcgggaacga gaagagcggc ctctccgagg aggagctctc cgcttgctcc ctcgccgtcc
     4201 acataccgac ctcttcggcc tttccctccc tgaacctcgc ccaggccgtc cagatcgccg
     4261 tctacgaggt ccgccgcgcg gccctgggcg ggcggaaggg ctcctacgag cccgcccccc
     4321 gcgcggccgt ggaggaggcc gtcggccgca tgtcgcgccg cctcgaggag ctgggcttct
     4381 tccgcctgaa ccagggcgcc gacctcaagg ccttcctccg cgacaccgcc gagcg
//
LOCUS       DAOOSJ010000221         4426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12425_length_4426_cov_5.972546, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000221 DAOOSJ010000000
VERSION     DAOOSJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12425_length_4426_cov_5.972546"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..134
                     /locus_tag="PLG14_10135"
     CDS             6..134
                     /locus_tag="PLG14_10135"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOX49531.1"
                     /translation="MANDEEKGNIILMHAIGSNVSGQLGSVIAGGMVLALVPLLVG"
     gene            150..305
                     /locus_tag="PLG14_10140"
     CDS             150..305
                     /locus_tag="PLG14_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49532.1"
                     /translation="MDQLIQNLRYESTTVQALGVTVGGLVGVFATLGVFFLVIWISGK
                     LGKKSAE"
     gene            674..1843
                     /gene="trpB"
                     /locus_tag="PLG14_10145"
     CDS             674..1843
                     /gene="trpB"
                     /locus_tag="PLG14_10145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOX49533.1"
                     /translation="MKHYDASLSMKSGFFGRFGGRYVPEALVPRLSALEAEFGRAIAD
                     PSFGEEFRRYLKDYVGRPSPLYRAERLSAEVGATIYLKREDLNHTGAHKINNTIGQIL
                     LARRMGAARVIAETGAGQHGVATATACALFGLSCTVYMGAEDARRQAPNVERMKLLGA
                     EVRTTDAGTATLKDAVDAALGAYAADPEAFYCLGSAVGPHPYPSIVRHFQSVIGTEAR
                     AQILEAEGRLPDAIFACAGGGSNAIGLFSAFLDDEGVEIFGAEGGGTDVAGLKTAATL
                     SRGAPCVFQGTFSYCLVDEGGNPVDAYSVSAGLDYPGIGPEHAHLKETGRVTYAPVMD
                     DEAIEAFGKLSRLEGVIPAIESAHAVADAMKRLAGTGKLAIINVSGRGDKDLGRF"
     gene            complement(1999..3213)
                     /locus_tag="PLG14_10150"
     CDS             complement(1999..3213)
                     /locus_tag="PLG14_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOX49534.1"
                     /translation="MKAHAITDRIYALHADIETDDLFEGIWPIPDGVSLNSYLVKGDK
                     TALIDLVRDWTEAPKVLEEELAGIGVKLSDVDYLVLNHLEPDHTGWLAEYKRLNPKVE
                     ILATPKGVELVKSFYKITEGLRAVKSGDSLDLGEGMVLRFEEAPNVHWPETMVTFEPS
                     SGVLFACDAFGSYGKLGDRVFDDQFSPEEHAFFEKESLRYYANIVASFSPFVKKAVAK
                     LAALDVKVVAPSHGIVWRKDPKAIIDRYSRYADYMEGPREKEICVVWGSMYGNTKKGL
                     DAVIAGIEDEQVPYSVHRVPNEDVSYVLADAYKSAGIVIAMPTYEYKMFPPMAYVLNL
                     FERKHVWGRKAFRLGSWGWIGGAKKEYEALVAPLKWDCLEAEEWAGAPDAERLAALRE
                     KGRELARKVKEA"
     gene            complement(3489..3701)
                     /gene="rpmE"
                     /locus_tag="PLG14_10155"
     CDS             complement(3489..3701)
                     /gene="rpmE"
                     /locus_tag="PLG14_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOX49535.1"
                     /translation="MKADIHPKYEMTKITCACGSVIETRSTVKNIEVEICSACHPFFT
                     GKQKLVDTAGRIERFNRKYGIKSSEK"
     gene            complement(3804..>4426)
                     /gene="rho"
                     /locus_tag="PLG14_10160"
     CDS             complement(3804..>4426)
                     /gene="rho"
                     /locus_tag="PLG14_10160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOX49536.1"
                     /translation="MERTVKAEVISSTFDEQATRHVQVSEMVLDKAKRLVEHGKDVVI
                     LLDSITRLARAYNQTVPTSGKILSGGVDSNALHKPKRFFGAARNIEGGGSLTIIATAL
                     IDTGSRMDEVIFEEFKGTGNMEINLDRRLSDRRLFPAINIKKSGTRKEELLLTDEELQ
                     KIWVLRKVISPMDDVEIMELLIDKMMKSKNNDAFLRSMNTPLGGGD"
BASE COUNT          658 a         1492 c         1493 g          783 t
ORIGIN      
        1 agaagatggc caacgacgag gagaagggca acatcatcct gatgcacgcc atcggctcca
       61 acgtctccgg ccagctcggc tcggtcatcg cgggaggcat ggtcctcgcc ctcgtccccc
      121 tcctcgtggg ctaagtaagg agcgcaatga tggatcagct cattcagaac ctcaggtacg
      181 agagcaccac cgtccaggcc ctgggcgtca ccgtgggcgg cctcgtcggc gtcttcgcca
      241 ccttgggcgt gttcttcctc gtcatctgga tctcgggcaa gctcgggaag aagtcggccg
      301 agtaggcctt cgcgcgctcc ggggcgggct ttggcccgcg gcccggaacg aggcggcccg
      361 ccccctgccg gggcgggccg tttttatatg cagaaacagc ttgacggccc tgggcgatcc
      421 aggtaagatg ctacgcggag aaacaagatg aagcagcact tcagcgccgt ccttggatct
      481 agacccgccg ccgcattctc ctcctcccct gcccaccacg gctccatctt ctgggcctgg
      541 ccccgctaac acccctccat cgcccgcgcg gcccgggaca ggccgcctct ccgagcggat
      601 agcgggactg cagcccgtcg ggccgtcccc cgcctagctt ccgcatgctt tggcaaaggc
      661 ccaggagccc ctcatgaagc actacgacgc ctccctctcg atgaagtccg gtttcttcgg
      721 ccgcttcggc ggcaggtacg tccccgaggc cctcgtcccc aggctctccg ccctggaggc
      781 ggagttcggg cgcgcgatcg ccgacccctc cttcggcgag gagttccgcc gctacctcaa
      841 ggactatgtg ggcaggccga gcccgctcta ccgggcggag cggctctccg cggaggtcgg
      901 cgcgacgatc tacctgaagc gcgaggacct gaaccacacg ggcgcgcaca agatcaacaa
      961 caccatcggc cagatcctcc tggcccgccg gatgggcgcg gcccgcgtca tcgcggagac
     1021 cggcgccggg cagcacgggg tcgcgacggc gacggcctgc gccctcttcg gcctctcctg
     1081 cacggtctac atgggagcgg aggacgcgag gaggcaggcg cccaacgtcg agcggatgaa
     1141 gctcctgggc gccgaggtgc ggaccacgga cgcaggcacg gcgacgctca aggacgcggt
     1201 cgacgcggcc ctgggcgcct acgccgccga ccccgaggcc ttctactgcc tcggctcggc
     1261 cgtcggcccc catccctatc cctccatcgt caggcatttc cagtcggtga tcgggaccga
     1321 ggcccgggcg cagatcctgg aggccgaggg ccgcctcccc gacgccatct tcgcctgcgc
     1381 gggcggcggc tcgaacgcga tcggcctctt ctcggccttc ctggacgacg agggcgtgga
     1441 gatcttcggc gccgagggcg gcgggaccga cgtagcgggg ctcaagaccg ccgcgaccct
     1501 gagccgcggc gcgccctgcg tcttccaggg gaccttctcc tactgcctcg tggacgaggg
     1561 cgggaatccc gtggacgcct actccgtctc agcgggcctc gactacccgg gcatcggccc
     1621 cgagcacgcc cacctcaagg agacgggtcg cgtcacctac gcgcccgtga tggacgacga
     1681 ggcgatcgag gccttcggga agctctcgcg cctggaggga gtcatcccgg cgatcgagag
     1741 cgcccacgcc gtcgccgacg cgatgaagcg cctcgcgggc acgggcaagc tcgcgatcat
     1801 caacgtctcg gggcggggcg acaaggacct ggggaggttc tagagggcgg cccaaggccg
     1861 cggtccgctg ctgcggtccg tcgccgcggt ccgtcgccgc ggtccggcgc ggtttgacgc
     1921 cgccgtgacg cggccaaaaa agcgaaggga ggagccgctc ggctcctccc ttccgcgttc
     1981 cacggcggcg cgcccgcctc aggcttcctt gaccttgcgc gcgagctcgc ggcccttctc
     2041 gcgcagggcc gcgagcctct ccgcgtcggg ggcgcccgcc cactcctcgg cctcgaggca
     2101 gtcccacttc aggggggcga cgagggcctc gtactccttc ttggcgccgc cgatccagcc
     2161 ccagcttccg agccggaagg ccttgcggcc ccacacgtgc ttcctctcga agaggttgag
     2221 gacgtaggcc atgggcggga acatcttgta ctcgtaggtg ggcatggcga tgacgatccc
     2281 cgcgctcttg taggcgtcgg cgaggacgta ggagacgtcc tcgttgggca cgcggtggac
     2341 gctgtagggc acctgctcgt cctcgatgcc ggcgatgacc gcgtccaggc ctttcttcgt
     2401 gttgccgtac atggagcccc agacgacgca gatctccttc tcccgggggc cttccatgta
     2461 gtccgcgtac ctcgagtagc ggtcgattat ggccttcggg tccttcctcc agacgatgcc
     2521 gtggctcggc gcgacgacct tgacgtccag ggcggcgagc ttcgccacgg ccttcttcac
     2581 gaaggggctg aagctcgcga cgatgttggc gtagtagcgg aggctctcct tctcgaagaa
     2641 ggcgtgctcc tcgggcgaga actggtcgtc gaagacgcgg tcgccgagct tcccgtagct
     2701 cccgaaggcg tcgcaggcga agaggacgcc ggaggagggc tcgaaggtga ccatcgtctc
     2761 aggccagtgg acgttgggtg cctcctcgaa gcgcaggacc atgccctctc ccaggtccag
     2821 gctgtcgccc gacttcacgg cccggaggcc ctccgtgatc ttgtagaagg acttcacgag
     2881 ctcgacgccc ttcggggtag cgagaatctc caccttgggg ttgagccgct tgtactcggc
     2941 gagccagccc gtgtggtccg gctccaggtg gttcaggacg aggtagtcga cgtcggagag
     3001 cttgacgccg atcccggcga gctcctcctc caggaccttg ggcgcctcgg tccagtcgcg
     3061 caccaggtcg atgagggcgg tcttgtcgcc cttcacaagg taggagttga gggagacgcc
     3121 gtcggggatg ggccagatgc cctcgaagag gtcgtcggtc tcgatgtcgg cgtggagcgc
     3181 gtagatgcgg tcggtgatcg cgtgggcttt catttcggtc tccgtcgtgg gatgctgcct
     3241 tggcggcgcc tcggggggcc gcgcttcaag tatacaggcg ctgcgtcggc tgggcaacgc
     3301 cggctcgtgg aagagccgcc gcgtcggctg ggcaacgccg ccccgctccg gcaggccgtc
     3361 ctcggccacg cattgtctgg cggcgggccg gccggccggc caaggcgcct ctccggctgg
     3421 cgcccgggcc gcgcgggcaa aaaaaggccg tccccggggg gacggcgctt ttcgcgaact
     3481 ggcgagaatt acttctcgga cgacttgatg ccgtacttcc tgttgaagcg ctcgatgcgt
     3541 ccggcggtgt ctacgagctt ctgcttgccg gtaaagaagg ggtggcaggc cgagcaaatt
     3601 tcgacctcga tgttctttac cgtcgaacgg gtctcgatga cactaccgca ggcgcaggtg
     3661 atcttcgtca tctcatactt gggatgaatg tcagccttca tttcactacc tcccgcgttt
     3721 cgtgcctgaa aaccggcctt gtggacggcg ttcttcctat aagcctcccg gagggccttg
     3781 gcccgccggg aagttccgct cccctagtct ccgccgccca agggcgtgtt catcgaccgg
     3841 aggaacgcgt cattattctt ggatttcatc attttgtcaa tgagcagctc catgatctcc
     3901 acgtcgtcca tggggctgat caccttgcgc aggacccaga tcttctggag ctcctcgtcc
     3961 gtgaggagga gctcctcctt gcgcgtgccg gacttcttga tgttgatcgc ggggaagagg
     4021 cgcctgtcgg agagcctgcg gtccaggttg atctccatgt tgcccgtgcc cttgaactcc
     4081 tcgaagatga cctcgtccat gcggctgccc gtgtcgatga gggcggtcgc gatgatcgtg
     4141 agggagccgc ccccctcgat gttgcgcgcg gcgccgaaga agcgcttggg cttgtggagg
     4201 gcgttggagt ccacgccgcc cgagaggatc ttgccggagg tcggcacggt ctggttgtag
     4261 gcgcgggcga ggcgggtgat cgagtcgagg aggatgacga cgtccttccc gtgctcgacg
     4321 aggcgcttgg ccttgtccag gaccatctcg gagacctgga cgtggcgggt cgcctgctcg
     4381 tcgaaggtgg aggagatgac ctccgccttg acggtgcgct ccatgt
//
LOCUS       DAOOSJ010000222         4343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12738_length_4343_cov_6.187966, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000222 DAOOSJ010000000
VERSION     DAOOSJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_4343_cov_6.187966"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PLG14_10165"
     CDS             <1..1382
                     /locus_tag="PLG14_10165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX49537.1"
                     /translation="AADGDAARTPLPRFLLLSCADAGIERELGANKRLAKWLAPCPGG
                     FRVLLTERGTVKRELLRLGWPVKDVAPLTPGDPLSFSLLPARRSGGPFGLRPYQEDAL
                     SAFLGGGLPGSGYGVVVLPCGAGKTIVGMAAMSAIGRKTLIVTTNVAAVHQWIDELRD
                     KTDLGEDAIGEYTGAEKTVRPVTVATYQVLTWRPDKESDFPHFDLFRRENWGLIVYDE
                     VHLLPAPVFRVVAEIQAVRRLGLTATLVREDGREEDVFSLIGPKRYDVPWKDLERKGF
                     IAEAYCREIRIPLPPEEELRYAVSEPREKHRLAAENTAKYEAVRELVENHPDDPVMVI
                     GQFLDQLGRLSKALGAPLITGKTPNPERERIYADFRKGTTRVIVVSKVANFAIDLPDA
                     SVAIQVSGSFGSRQEEAQRLGRILRPKKRNSFFYSLVTRYTVEEEFAANRRKFLTEQG
                     YRYQIEAWE"
     gene            1379..4033
                     /locus_tag="PLG14_10170"
     CDS             1379..4033
                     /locus_tag="PLG14_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49538.1"
                     /translation="MSERTRPGPRIDEELWTAALLSSDTEALIAAARNYLGPVRTPYD
                     KRELVSRLAAFLRRPDSREGAVALLDLLDARIVGSLLVAGPLSEPELRSLFVGELPLF
                     DLGIRVSNLLDRLLLFRFETGGRRYLAVNPILAEELGRAVLDPVLLFGAAAEGAQGPG
                     AGEGLAAEAAPATGAAAHPAEGVAAPSAGASTDGDVAASGTAATRSAAAQAKGLEPKD
                     GLLDAEAATAFFLFLHHAPGSLRKGGGLTKRASERLAALFPGLAGGGAGGAAAAEAAV
                     GGESRFDAMARALAAAGALGGGEEGRGPDPAAYAALAAEWGAALPAFLATALARSGAE
                     EAAMAEGRASLEAEASLVAAALAAAPPGLGFSKGGLSRWLGLASRRAAQTAVPRPRSA
                     AAQAEPPLIEGAEAERLLAALLALGLAADRDGGIRLRAPWRDSGRSEGGAGEARERGA
                     AESLAAEGNSAEGNAAEAGKPVLVAEGSHALHLLPEASLEDRLFVGRLARPTAFGKVW
                     SLELDRDSARRGFAAGLGAKESLARLEALAGRPLPQSLSFSLASWEEEYRALRLYRGF
                     VLVADERLRQIVERSERLKPLLAERLAPGVYVLAAASPEEAAAALREAGLEPPPEVRR
                     ATPEGWAESEPAARGAGALRGPGARGSALALRIESAVAPLAGLAAEAAPGAAGGAAAS
                     GGAGGRSAGAAGRLDPAARVASLKAALAALAAGGAAERAEAARAAAGPGGAEGAARGR
                     APAPDRGAADRGSLASGAHGPERGPEVLRELEDRIERRLVLSERQLAQADARPERIEA
                     AGLDYMGKVRIVERALRTSGDRLEVLYRLPGADPARALLRPVRLDKTDKGLVLEAEDL
                     ATGGPVRVPLGAMSSVKRMRASLFGEEA"
     gene            4030..>4343
                     /locus_tag="PLG14_10175"
     CDS             4030..>4343
                     /locus_tag="PLG14_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOX49539.1"
                     /translation="MKNNSQAYQNQPKAEAGYQGQLKLAVLVDADNTQASIIEGLLGE
                     VAKYGIASVKRIYGDWTSTNLRSWKDKLLEFAIQPIQQFSYTTGKNATDSAMIIDAMD
                     LL"
BASE COUNT          560 a         1699 c         1566 g          518 t
ORIGIN      
        1 ccgccgcgga cggcgacgcc gcgaggaccc cgctgccccg ctttctcctc ctctcctgcg
       61 cggacgccgg gatcgagcgg gagctcggcg cgaacaagcg cctcgcgaaa tggctcgcgc
      121 cctgccccgg cggcttccgc gtcctcctca ccgagcgggg cacggtgaag cgcgagctcc
      181 tccggctcgg ctggccggtg aaggacgtcg cgccgctcac gcccggcgac cccctctcct
      241 tctccctcct ccccgcccgc cgctcgggcg gccccttcgg cctcaggccc taccaggagg
      301 acgccctgtc cgccttcctg ggcggcggcc tccccggctc gggctacggc gtcgtcgtcc
      361 tgccctgcgg cgcgggcaag accatcgtcg gcatggccgc gatgtccgcc atcggccgca
      421 agaccctcat cgtgacgacc aacgtggccg ccgtccacca gtggatcgac gagctccgcg
      481 acaagaccga cctgggcgag gacgcgatag gcgaatacac cggcgcggag aagacggtcc
      541 ggcccgtgac cgtcgccacc taccaggtcc tgacctggcg ccccgacaag gagtcggact
      601 tcccccactt cgacctcttc cgccgcgaga actggggcct catcgtctac gacgaggtcc
      661 acctcctgcc cgcgcccgtc ttccgcgtcg tcgcggagat ccaggcggtg cgccgcctgg
      721 gcctcaccgc gaccctggtg cgcgaggacg gccgcgagga ggacgtcttc agcctcatcg
      781 ggccgaagcg ctacgacgtg ccctggaagg acctggagcg gaagggcttc atcgccgagg
      841 cctattgccg cgagatccgg atcccactcc ctcccgagga ggagctgcgc tacgcggtct
      901 ccgagccgcg ggagaagcac cggctggccg ctgagaacac ggcgaagtac gaggcggtgc
      961 gcgagctcgt ggagaaccat cccgacgacc ccgtcatggt gatcggccag ttcctggacc
     1021 agctcggccg cctctccaag gccctgggcg cgcccctcat caccgggaag acgcccaacc
     1081 ccgagcggga gcggatctac gccgacttcc gcaaggggac gacgcgggtc atcgtggtct
     1141 ccaaggtcgc caacttcgcg atcgacctgc ccgacgcctc cgtggccatc caggtctccg
     1201 gcagcttcgg ctccaggcag gaggaggccc agcgcctggg gcggatcctc cggcccaaga
     1261 agaggaactc cttcttctac tccctcgtca cgcgttatac ggtcgaggag gagttcgcgg
     1321 ccaaccggcg caagttcctg accgagcagg gctaccgcta ccagatcgag gcctgggaat
     1381 gagcgagcgg acgcgcccgg ggccgcgcat cgacgaggag ctctggaccg ccgccctcct
     1441 gtcctccgac acggaggccc tgatcgcggc cgcccgcaac tacctgggcc cggtgcgcac
     1501 gccctacgac aagcgcgagc tcgtctcgcg gctcgccgcc ttcctccggc ggcccgactc
     1561 ccgcgagggc gccgtcgccc tcctcgacct cctggacgcg cggatcgtcg ggagcctcct
     1621 cgtcgcgggg cccctctccg agcccgagct caggagcctc ttcgtcgggg agctgcccct
     1681 cttcgacctc ggcatccgcg tctcgaacct cctggaccgc ctcctcctct tccgcttcga
     1741 gacgggcggc cgccgctacc tggcggtgaa cccgatcctc gcggaggagc tggggcgggc
     1801 cgtcctcgac cccgtcctcc tcttcggcgc cgccgcggag ggcgcccagg ggccgggagc
     1861 cggcgagggg ctcgccgccg aagcggctcc ggcgaccggc gccgcggctc acccggcaga
     1921 gggtgtggcg gcgccaagcg ctggcgcgag caccgacggg gacgtcgccg caagcgggac
     1981 tgcggcgacc cggtccgccg cggcgcaggc taaggggctc gagccgaagg acggcctcct
     2041 ggacgccgag gcggcgacgg ctttcttcct tttcctccac catgccccgg ggagcctcag
     2101 gaagggcggc ggcctcacga agcgcgcctc cgagcggctg gccgcgctct tccccggcct
     2161 ggcgggcggc ggggccggcg gggcggccgc cgcggaggcg gccgtcgggg gggagagccg
     2221 cttcgacgcg atggcgcggg ccctcgccgc ggccggagcc ctggggggag gggaggaggg
     2281 gcgaggcccc gatccggcgg cctatgccgc cctggccgcg gagtggggcg cggccctgcc
     2341 cgccttcctc gccacggccc tggcccggag cggcgcggag gaggcggcca tggccgaggg
     2401 ccgcgcctcg ctggaggccg aggcttccct cgtggccgcc gccctcgccg ccgcgcctcc
     2461 cgggctcggc ttctccaagg gcgggctctc ccgctggctg ggcctcgcct cgcgccgggc
     2521 ggcccagacg gcggtccccc ggcccaggtc ggccgccgcc caggccgagc cgcccctcat
     2581 cgagggcgcg gaggccgagc gtctcctcgc cgccctcctg gccctgggcc tggccgcgga
     2641 cagggacggc ggcatccggc tccgcgcgcc ctggcgggac tccgggcgca gtgaaggcgg
     2701 cgcgggcgag gcgcgcgagc gcggggcggc cgaaagccta gcggccgagg gcaactcggc
     2761 cgagggcaac gcggccgagg cggggaagcc cgtcctcgtc gccgagggct cgcacgccct
     2821 ccacctcctc cccgaggcga gcctcgagga ccggctcttc gtgggccgcc tcgcgaggcc
     2881 cacggccttc gggaaggtct ggagcctcga gctcgaccgc gacagcgcca ggaggggctt
     2941 cgccgccggc ctgggcgcga aggagtctct cgcgcggctc gaggccctgg cgggaaggcc
     3001 cctcccgcag agcctcagct tctccctcgc ctcctgggag gaggagtacc gggcactcag
     3061 gctctaccgg ggcttcgtgc tcgtcgcgga cgagcggctc aggcagatcg tggagagatc
     3121 cgagcgcctg aagcccctcc tcgccgagag gctcgcgccc ggggtctacg tcctggccgc
     3181 cgccagcccc gaggaggccg ccgcagccct ccgcgaggcc ggcctggagc ccccgcctga
     3241 ggtccgacgg gccacgcccg agggctgggc cgagtccgag ccggctgccc ggggggccgg
     3301 cgcccttcgc ggaccgggcg cgcgcgggtc ggccctggcg ctccggatcg agtccgccgt
     3361 ggcgcccctg gccgggctcg ccgccgaggc ggcgcccggc gcggcgggcg gagcggcagc
     3421 gtccggcggc gctggcgggc ggagcgcagg cgcagccggc cgcctcgacc cggcggcgcg
     3481 cgtcgcctcc ctcaaggccg ccctcgcggc cctggccgcc ggcggggcgg ccgagcgggc
     3541 cgaggccgcg cgcgccgcgg ccggcccagg cggggcggag ggcgcggcgc ggggcagggc
     3601 tccggctccc gaccgcggcg cggcggaccg cggctccctc gcctccgggg cccatggccc
     3661 ggagcgcggg cccgaggtgc tgcgcgagct cgaggacagg atcgagcgcc gcctcgtcct
     3721 ctcggagcgc cagctcgccc aggccgacgc gcggcccgag cggatcgagg ccgcgggcct
     3781 cgactacatg gggaaggtgc gcatcgtcga gcgcgccctc cgcacttccg gcgaccggct
     3841 ggaggtgctc taccgcctcc ccggcgccga ccccgcgcgg gccctcctca ggccggtgcg
     3901 gctcgacaag accgacaagg ggctcgtcct cgaggccgag gacctcgcga ccggcggccc
     3961 cgtccgcgtg ccgctcggag ccatgagctc cgtgaagcgg atgcgcgcat ccctcttcgg
     4021 agaagaagca tgaagaacaa cagccaagca taccagaacc agcccaaggc cgaggccggc
     4081 taccagggcc agctcaagct cgccgtcctc gtcgacgccg acaacaccca ggcttccatc
     4141 atcgagggcc tcctgggcga ggtcgcgaag tacggcatcg cgagcgtcaa gcgcatctac
     4201 ggcgactgga cgagcacgaa cctccgctcc tggaaggaca agctcctcga gttcgccatc
     4261 cagcccatcc agcagttcag ctacacgacg ggcaagaacg cgaccgacag cgcgatgatc
     4321 atcgacgcga tggacctcct cta
//
LOCUS       DAOOSJ010000223         4337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12762_length_4337_cov_4.358944, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000223 DAOOSJ010000000
VERSION     DAOOSJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12762_length_4337_cov_4.358944"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLG14_10180"
     CDS             complement(<1..504)
                     /locus_tag="PLG14_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49540.1"
                     /translation="MRRREWIRFVSGRWFAAKKASGGSATSVLASAGIAVGVAALIVV
                     LGVMNGFQMGYVDSILEISSFHVRIEDERAGGPDERLLAELGGLPGARSVLPFAETQV
                     LLSTEEGRSMPIRVRAVPDDAAVRDPGLAPALGLEGRAFPPEEGIVLGAELARYLNLA
                     AGDEVELL"
     gene            complement(501..1643)
                     /locus_tag="PLG14_10185"
     CDS             complement(501..1643)
                     /locus_tag="PLG14_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49541.1"
                     /translation="MRKDARRASFSLAALARAGFALAGLAAAEQAWAEAPPPGEGRAS
                     AWFASDQGGFQGESIPALAPTGFCLWVGTEGGTETRILFKDGLETERRVTRKTASGSQ
                     ESVREGGVLREERKSGPSGQLQEELFYFESGLLRERRSYVYEDRRLSRVEAFDSGGAQ
                     VGSLSYLYDPRGRLSELQAAGSFGEARSGLLGLSGGGVAAEWSILEGELLLRRFDQAG
                     RVASVEVREGDAVLRRESFAYEGASARPSLSLLEDLASGDGTEKAYDAAGRIIRLRLL
                     SKGAERSRTDYAYDAEGRLESERTRSGSSLSLRSLSYDGEGGLLREETRKDGLLFRTV
                     AFLKDGRVEEYFEGGALVLRATYEGAKKTKDEFFEGGQVVRVRSYP"
     gene            complement(1651..2526)
                     /gene="ftsY"
                     /locus_tag="PLG14_10190"
     CDS             complement(1651..2526)
                     /gene="ftsY"
                     /locus_tag="PLG14_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOX49542.1"
                     /translation="MKFAERIKALFGLGNRGDEDFFDELADLLVEGDLGASLAFAVAD
                     ELKASCRRQGISGPEAIKLELKRILAGYGKEARVEPEPGRLNVFLLLGVNGVGKTTSC
                     AKLARHYMKSGSAKGVILAAGDTFRAAAIDQIKIHGERLGLRVVAQRHGSDPGAVLYD
                     ALEAAKADGANLVLADTAGRMHTKLDLVRELGKIDKIVASRAEGANYRRLLAIDATTG
                     QNGLRQAEVFAGAVPIDGLVLTKLDSSAKGGLALALAKELGLPTAFVGRGEGYDDLEP
                     FRLGAFLDEFLGIEG"
     gene            complement(2539..4125)
                     /locus_tag="PLG14_10195"
     CDS             complement(2539..4125)
                     /locus_tag="PLG14_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49543.1"
                     /translation="MPGSGRRPAGGRAGHGLPPAPGLLPAALLAAFAACAALPPPADA
                     QAAQPGAAASAASKAAGQAVKPYVVAVAELSGRDLGSLSEPFLSILPRLLAAELSSLP
                     PRHEDGAYLAEAALRSSEAARFAAGAALAARLDEEALRSLEPGVDPLKRASQVAEARR
                     RTEEAAKALAELGSLGEAAAPPAASRASAQAEGAAPPASGSMPQAGTGPRTTLLWEGN
                     AGGGLFPATESDPSSLLRDKGVSLLVRGQARAFGSYLALDMECFDLALGRPILAFRAY
                     ADPADPGSLARDIAARVERAVAGRDFARVELKARPASALVLADGGLLGTGDGVLYRFE
                     PGILELAAAAPGRLPESLSLPLGLGDRKTASFDLKRASTGLVLVETDPPGAAVFLDGL
                     PAGKAPARLELEGRRAVASASAPGRESVSVVLQAEGESVARIELPPDDGLGPAGRIDL
                     AQRRFYKALGWFVLSLPATTLGYGIQGSYREAAARGYDPGMVSAYETSYYAFEIAAAA
                     SAALAVNALFRLAAYIRAAR"
     gene            complement(4128..>4337)
                     /gene="prfB"
                     /locus_tag="PLG14_10200"
     CDS             complement(4128..>4337)
                     /gene="prfB"
                     /locus_tag="PLG14_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704758.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOX49544.1"
                     /translation="KKEIAWGSQIRSYVFQPYTMVKDHRNKFFVGNVQAVMDGDIDSF
                     IESFLRWRWQGGTAGGAEAEDDEEL"
BASE COUNT          600 a         1532 c         1605 g          600 t
ORIGIN      
        1 gaggagctcc acctcgtcgc ccgccgccag gttcaggtag cgcgcgagct cggcccccag
       61 gacgatgcct tcctcggggg gaaaggcccg gccttccagg cccagggcgg gggccaggcc
      121 gggatcgcgg accgcggcgt cgtcggggac ggctcggacg cggatgggca tgctcctccc
      181 ctcctccgtg gagaggagta cctgggtctc ggcgaaggga aggacgcttc ttgcgcccgg
      241 caggcccccg agctcggcga gcagcctctc gtcggggccg cccgcgcgct cgtcctcgat
      301 cctcacgtgg aagctcgaga tctccaggat ggagtccacg tagcccatct ggaagccgtt
      361 catcacgccc aggacgacga tgagggccgc gacgccgacg gctatgcctg ccgaggcgag
      421 gacggaggtc gccgagcctc cggaggcctt cttcgccgcg aaccagcggc cggagacgaa
      481 gcggatccat tcccgccgcc tcatggatag ctcctgacgc ggaccacctg tcccccttcg
      541 aagaactcgt ccttggtttt cttcgcgcct tcgtaggtcg cgcggaggac gagggcgccg
      601 ccctcgaagt actcctccac ccgcccgtcc ttcaggaagg cgaccgtcct gaaaagaagg
      661 ccgtccttcc tcgtctcctc gcggaggagg ccgccctccc cgtcgtagga gaggctgcgg
      721 agggacaggg aggagccgct cctcgtccgc tcgctctcga gccttccctc ggcgtcgtag
      781 gcgtagtccg tccgggagcg ctcggctccc ttggacagga ggcggaggcg gatgatccgt
      841 cccgccgcgt cataggcctt ctccgttcca tcccccgagg caaggtcctc gaggagggag
      901 agggagggcc tcgcggaggc cccttcgtag gcgaagctct cccggcggag tacggcgtcg
      961 ccctcgcgga cctccacgga ggcgacgcgc cccgcctggt cgaagcgcct gaggagaagc
     1021 tcgccctcca ggatggacca ttcagcggcg actccgccgc cggagaggcc gaggaggccg
     1081 gacctggcct cgccgaagct ccccgccgcc tggagctcgg agagcctccc ccgcggatcg
     1141 tagaggtagg agagcgagcc gacctgcgcg ccgccggagt cgaaggcttc gaccctcgag
     1201 agcctccgat cttcgtagac ataggagcgc cgctcgcgga ggaggcccga ctcgaagtag
     1261 aaaagctcct cctggagctg gccgctcgga cccgacttcc tctcctcgcg gaggacgccg
     1321 ccctcgcgga cgctctcctg gctgcccgag gccgtcttgc gcgtgacgcg gcgctcggtc
     1381 tccaggccgt ccttgaagag gatccgcgtc tcggtcccgc cctcggtccc gacccagagg
     1441 caaaagccgg tcggcgcgag tgcggggatg gactcgccct ggaagccgcc ctggtcggag
     1501 gcgaaccagg ccgaagcccg gccctcgccc gggggcggcg cctccgccca ggcctgctcc
     1561 gccgcggcga ggcccgccaa ggcgaatccc gcccgggcca gggctgcgag ggaaaaagag
     1621 gcgcgtcgcg cgtctttccg catggaaagc ctagccctcg atccccagga actcgtcgag
     1681 gaaggcgcca agcctgaagg gctccaggtc gtcgtagccc tcgcccctgc cgacgaaggc
     1741 cgtcggcagg ccgagctcct tggcgagggc cagggccagg cctcccttgg cggaggagtc
     1801 gagcttcgtg aggacgaggc cgtcgatggg caccgcgccc gcgaagacct cggcctggcg
     1861 gaggccgttc tggccggtgg tcgcgtcgat cgcgaggagg cggcggtagt tcgcgccctc
     1921 cgcccgggag gcgacgatct tgtcgatctt gccgagctcg cgcaccaggt cgagcttcgt
     1981 gtgcatccgc cccgccgtgt ccgcgaggac gaggttcgcg ccgtcggcct tcgccgcctc
     2041 cagggcgtcg tagaggacgg cgcccgggtc ggagccgtgc cgctgggcga cgaccctgag
     2101 gcccaggcgc tcgccgtgga tcttgatctg gtctatggcc gcggcccgga aggtgtcgcc
     2161 ggccgcgagg atgaccccct tggcgcttcc gctcttcatg tagtgccggg cgagcttcgc
     2221 gcagctcgtc gtcttgccga cgccgttgac gcccaggagg aggaagacgt tgaggcggcc
     2281 gggctcgggc tcgaccctcg cctccttgcc gtagcctgcg aggatgcgct tgagctcgag
     2341 ctttatcgcc tcggggccgg agatcccctg cctgcggcag gaggccttga gctcgtcggc
     2401 gacggcgaag gccagcgagg ccccgaggtc gccctccacg aggaggtcgg cgagctcgtc
     2461 gaagaagtcc tcgtcgcccc ggttgccgag gccgaagagg gccttgatgc gttcggcgaa
     2521 tttcatcgtt ccatgctcct agcgcgccgc cctgatgtag gcggcgaggc ggaatagcgc
     2581 gttgacggcc agggcggcgc tggccgccgc ggcgatctcg aacgcgtagt agctcgtttc
     2641 gtaggcggag accatgcccg ggtcgtagcc cctggcggcg gcctcgcggt agcttccctg
     2701 gatgccgtag cccagagtgg tcgcggggag ggagaggacg aaccagccca gggccttgta
     2761 gaagcggcgc tgggccaggt cgatgcgccc cgcggggcca aggccgtcgt ccggcggcag
     2821 ctctatcctc gcgacggact cgccctcagc ctgaaggacg acggagacgc tctcgcggcc
     2881 gggcgccgag gcgctcgcga cggcgcgtcg accctcgagc tccagccgcg cgggagcctt
     2941 gcccgcggga aggccgtcca ggaagacagc cgctccgggg gggtcggtct ccacgaggac
     3001 gaggcccgtc gaggcccgct tgaggtcgaa ggaggcggtc ttcctgtcgc cgagtcccaa
     3061 gggcagggaa aggctttcgg ggagcctccc cggcgccgcg gcggccagct cgaggatccc
     3121 cggctcgaag cggtagagga cgccgtcccc tgttcccagg agccctccgt cggcgaggac
     3181 gagggccgag gcgggcctcg ccttgagctc gacgcgggcg aagtccctgc ccgcgacggc
     3241 cctctccact cgcgccgcga tgtcgcgggc gagggagccg gggtcggcgg gatcggcgta
     3301 ggcgcggaag gccaggatgg gccttcccag ggcgaggtcg aagcactcca tgtcgagggc
     3361 gaggtaggag ccgaaggccc tggcctggcc cctcacgagg aggctcacgc ccttgtcgcg
     3421 gaggaggctc gagggatctg actcggtcgc ggggaagagg ccgcccccgg cgttgccctc
     3481 ccacaggagg gtcgtgcgcg gcccggtccc ggcttgaggc atcgacccgg aggcgggagg
     3541 cgccgccccc tccgcctggg ccgacgcccg cgacgcggcc ggcggcgccg cggcctcgcc
     3601 caaggagcca agctcggcca gggccttggc ggcctcctcc gtcctccttc tcgcctcggc
     3661 gacctggctc gcccgcttca ggggatcgac gccgggctcg aggctccgca gggcctcctc
     3721 gtcgaggcga gcggccagcg cggctccggc ggcgaagcgc gcggcctcgg agctccgaag
     3781 ggccgcctcg gcgaggtagg ccccgtcctc gtggcgcgga ggcaggctcg agagctcggc
     3841 ggcgaggagg cgaggcagga tggagaggaa gggctcggag agggacccga gatccctgcc
     3901 ggagagctcg gcgacggcga cgacgtaggg cttgaccgcc tggccggccg ctttcgaggc
     3961 cgccgaggcc gccgcgcccg gctgggcggc ctgggcatcc gcgggcggcg gaagcgccgc
     4021 gcacgccgcg aaggcggcga gaagggccgc ggggaggagg cccggcgcgg gagggagccc
     4081 gtgaccggcc ctcccgcccg cgggcctcct gcctgagccc ggcatcccta gagctcctcg
     4141 tcgtcctccg cctcggcgcc gccggcggtt ccgccctgcc agcgccagcg gaggaaggac
     4201 tcgatgaagg agtcgatgtc gccgtccatc acggcctgga cgttgcccac gaagaacttg
     4261 ttccggtggt ccttcaccat cgtgtagggc tggaagacgt aggagcggat ctgggagccc
     4321 caggcgatct ccttctt
//
LOCUS       DAOOSJ010000224         4327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12812_length_4327_cov_5.250936, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000224 DAOOSJ010000000
VERSION     DAOOSJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_4327_cov_5.250936"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLG14_10205"
     CDS             complement(<1..256)
                     /locus_tag="PLG14_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX49545.1"
                     /translation="MSKAAWSPNKKLLLVFGVFLAAYFIPWEAPRLLGATSEAFLMLG
                     EYARQHVLLCLVPAMFIAGAITVFLNQQAVMRYLGPDANRL"
     gene            complement(302..565)
                     /locus_tag="PLG14_10210"
     CDS             complement(302..565)
                     /locus_tag="PLG14_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOX49546.1"
                     /translation="MSDRQEGVMKIQVLGSGCQKCKLLEQHVREAVAELGLAAEVEKV
                     TDLDAIMEMGVMMTPALAVDGEVKSVGKVLTKDQVMTHLKGGK"
     gene            complement(562..1098)
                     /locus_tag="PLG14_10215"
     CDS             complement(562..1098)
                     /locus_tag="PLG14_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX49547.1"
                     /translation="MKPLLKRYAGAAAAALAFAAFVLLFPAWRGKALDSMVFQTKTML
                     LVIPPIFILLGLLDAWVPREKMIRYMGEGSGLKGRVLAFLLGSFAAGPLYGAFPFAAV
                     LMKKGAAFSNILIFIGAWSTTKIPMLLFEMAALGKRFALSRLAVDIVGIAVIAWAIEL
                     ALPRKEVDRLYAEAEKTE"
     gene            complement(1095..1577)
                     /locus_tag="PLG14_10220"
     CDS             complement(1095..1577)
                     /locus_tag="PLG14_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOX49548.1"
                     /translation="MDTLFLYVLAAAGLALSLFKDRAKTRMALKKAWKAFEGILPQFL
                     AVLFLVAAALAVLDTATISRFLGSGSGLPGILGASFVGAITLIPGFVAFPAAAALLKG
                     GAGATQIAAFVSSLMMVGVVTLPMEMKFFGRQAALLRNALAWLFSIAAAFFVGWAVSL
                     "
     gene            1667..1996
                     /locus_tag="PLG14_10225"
     CDS             1667..1996
                     /locus_tag="PLG14_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOX49549.1"
                     /translation="MDSQSLRRYELKAEMFKALAHPMRIAMLDKLRARPWCVCELAAE
                     VGVDKSVASKHLSQLKAAGLVDDEKRGTLVEYRLVAPCVLDLAACAETTILEHRRQRL
                     GLGSSSS"
     gene            complement(2119..2928)
                     /locus_tag="PLG14_10230"
     CDS             complement(2119..2928)
                     /locus_tag="PLG14_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX49550.1"
                     /translation="MTGPHIEAQGLCYAYPDGTSALDSVSFSIPRGECVGLVGANGAG
                     KSTLLMLLAGLAFARSGTISLGGEVVERRSLASIRRKLGFVFQDPDDQLFMTTVRDDV
                     AFGPRNMGLPPEEVAERCAQALATVGIPHLAGRPPFRLSGGEKRAAALASILSMEPEA
                     LALDEPTAALDPRARRRLIGILGGLPQTRLVATHDIDLVYELCDRVIVLSGGRVAAFG
                     PTDLVVGDEALMERAGLEPPLALQACPRCGGSKAALRLRVSASGDEDCRGA"
     gene            complement(2925..3713)
                     /locus_tag="PLG14_10235"
     CDS             complement(2925..3713)
                     /locus_tag="PLG14_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOX49551.1"
                     /translation="MRIRELEGLSEGDSPVHGLHPLAKLAATLLYLVAVTSFPNKEVS
                     RLLPFALFPIVVASAAELPARPILARLLFASPLILGIGALNPFFDRALVAVGPFAVPG
                     GWLVFASLALKGALTASAAIILVGTTGAGGVGEALLLLGLPRIFVTQFLLTFRYLSLL
                     MEETARVLRAHELRSLGRRGIGKEARGSLPGGVLVRTYERGMRVYEAMRLRGFSGDLP
                     RDPGRRLGARDLAYLLSWAAVFLVLRTVDLPEAIGSFLIKAVSR"
     gene            complement(3810..>4327)
                     /locus_tag="PLG14_10240"
     CDS             complement(3810..>4327)
                     /locus_tag="PLG14_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOX49552.1"
                     /translation="IGLVEGLVTAAVVAYVAKASPDILERADSKEGLGQAPTRKVLAV
                     LAVGVLAVGGILSWFASANPDGLEWSMAKVAGRSELEAEGKVHDGLAELQGATAFMPD
                     YSFKPSASAAAATEVAATAAAETWPAVSAGTSAAGLVGGAMTLALAALVGWSIRAAKR
                     RKGPAQPAAGR"
BASE COUNT          743 a         1438 c         1550 g          596 t
ORIGIN      
        1 tgaggcggtt ggcatcgggg ccgaggtagc gcatgaccgc ctgctggttc aggaacaccg
       61 tgatggcgcc tgcgatgaac atggcgggca cgaggcagag gagcacgtgc tggcgggcgt
      121 actcgccgag catgaggaag gcctcgctcg tcgccccgag gaggcgaggc gcctcccagg
      181 ggatgaagta ggccgcgagg aagacgccga agacgaggag cagcttcttg ttgggggacc
      241 aggcggcctt gctcatgcct tggcccgcgc ttcgatcagg cccccgacga gctccgacat
      301 cttactttcc tcccttgaga tgcgtcatca cctggtcctt cgtgaggacc ttgcccacgc
      361 tcttcacctc gccgtcgacg gcgagggcgg gcgtcatcat cacgcccatc tccatgatcg
      421 cgtcgaggtc cgtgaccttc tcgacctccg ccgcgagtcc gagctcggcc accgcctcgc
      481 ggacgtgctg ctctaggagc ttgcacttct ggcaaccgga accaagcacc tgtatcttca
      541 tgacgccctc ctgtcgatcg ctcattcggt cttctccgcc tcggcataga ggcggtccac
      601 ttctttccgg ggaagcgcga gctcgatggc ccacgcgatg acggcgatgc cgacgatgtc
      661 gacggccagg cgcgagaggg cgaagcgctt gcccagcgcc gccatctcga agagcagcat
      721 cgggatcttc gtcgtggacc aggcgccgat gaagatgagg atgttggaga aggccgcgcc
      781 ctttttcatg aggacggcgg cgaaaggaaa ggctccgtag agcggccccg ccgcgaagga
      841 gccgaggagg aaggccagga cgcggccctt gaggcccgag ccctcgccca tgtagcggat
      901 catcttctcg cgcggcaccc aggcgtcgag gagcccgagg aggatgaaga tcggcggaat
      961 cacgaggagc atggtctttg tctggaagac catggagtcg agggccttcc cgcgccaggc
     1021 cgggaagagg aggacgaagg cggcgaaggc cagggcagcg gccgcggctc ccgcgtatcg
     1081 cttgagaagg ggcttcatag actcaccgcc catccgacga agaaggccgc ggctatcgag
     1141 aagagccagg cgagggcgtt cctcaggagg gccgcctgcc tgccgaagaa cttcatctcc
     1201 atcggaagcg tgacgacgcc caccatcatg aggctcgaca cgaaggccgc gatctgcgtc
     1261 gcgccggcgc cgcccttgag cagggccgcc gcggccggaa aggccacgaa gccggggatg
     1321 agggtgatgg cgccgacaaa ggaagcgccg aggatgcccg gcagccctga gccgcttccg
     1381 aggaagcggg agatcgtcgc cgtatcgagg acggccaggg ccgcggcgac caggaagagg
     1441 acggcgagga attgcggaag gatgccctcg aaggccttcc aggccttctt gagcgccatc
     1501 ctggtcttcg cgcggtcctt gaaaagggat aaggccaggc cggccgccgc cagcacataa
     1561 agaaacaggg tatccatggg cacctcttgt tgccaattat tgaaacattg cactgctttg
     1621 tcaagtcttc attgactttt gtttccaaac attgcaacat tgtggcatgg acagccagtc
     1681 attgcgccgc tacgagctga aagccgagat gttcaaggct ctcgcgcatc cgatgcgcat
     1741 cgccatgctc gacaagctca gggcgaggcc ttggtgcgtc tgcgagttgg ccgccgaggt
     1801 cggcgtcgac aaatccgtgg cctcgaagca cctctcgcag ctcaaggccg ccggactcgt
     1861 cgacgacgag aagcgcggca cccttgtcga ataccgcctg gtggcaccct gcgtcctgga
     1921 cctcgccgcc tgcgcggaga ccaccatact cgagcaccgc cgccagcgcc tgggcctggg
     1981 ttcctcctcg agctgaagga acacaggcgt cggccacgcc tggccccagg cctcgcggcc
     2041 tccgtctccg ctcttcaacc ccgctccttc aggcccctcc tcgggcgccg cggccgttct
     2101 caagcgccgc ggccgttctc aggcgccgcg gcagtcctcg tccccggacg ccgagacgcg
     2161 caagcggagt gcggccttgg agccgccgca gcggggacag gcctggaggg cgagcggcgg
     2221 ctccagcccc gccctctcca tgagcgcctc gtcgccgacg acgaggtccg tcggcccgaa
     2281 ggccgcgacc ctcccgcccg agagcacgat cacgcggtcg cagagctcgt agacgaggtc
     2341 gatgtcgtgg gtcgcgacca gtcgggtctg aggaaggccg cccaggatcc cgatgaggcg
     2401 ccgccgcgcc cgcggatcca gggccgcggt cggctcgtcg agggcgaggg cctctggctc
     2461 catcgagagg atcgaggcga gggcggccgc ccgcttctcg ccccccgaca ggcggaaggg
     2521 cgggcgcccc gcgaggtggg ggatgcccac cgtcgcaagc gcctgtgcgc atcgctcggc
     2581 gacttcctcc gggggcaggc ccatgttccg gggtccgaag gcgacgtcgt ccctgaccgt
     2641 ggtcatgaac agctggtcgt cgggatcctg gaagacgaag ccgagcttcc tccgtatcga
     2701 cgcgaggctc cgcctctcca cgacctcgcc gcccagggag atggtcccgc tccgcgcgaa
     2761 ggcgaggccg gcgagcagca tgaggagggt ggacttgcct gcgccgttcg cgcctacgag
     2821 gccgacgcac tcgccccgcg ggatcgagaa ggagaccgag tccagggcgc tcgttccgtc
     2881 gggataggcg tagcagaggc cctgcgcctc gatgtgggga ccagtcatcg cgacaccgcc
     2941 ttgatgagga aggatccgat cgcctcgggg agatccaccg tccggaggac gaggaagacc
     3001 gcggcccagg agaggaggta cgcgaggtcg cgcgcgccaa ggcgcctgcc cggatcccgc
     3061 ggaaggtcgc ccgagaagcc ccgcagcctc atcgcctcgt agacgcgcat gccccgctcg
     3121 taggtcctga cgaggacgcc gcccggcagg gagccccgcg cctccttccc tatgccgcgc
     3181 cggccgagcg accgcagctc gtgggcccgg agcacgcgcg cggtctcctc catgaggagg
     3241 gagaggtagc ggaaggtgag gaggaactgg gtgacgaaga tccgcggcag gccgaggagg
     3301 agcaaggcct cgcccactcc cccggcgccc gtcgtcccga cgaggatgat cgcggccgag
     3361 gccgtgagcg cgcccttcag ggccagggag gcgaagacga gccagccgcc cgggacggcg
     3421 aaggggccga ccgcgacgag ggcgcggtcg aagaaggggt tcagcgcgcc tatgccgagg
     3481 atgagggggg aagcgaagag gaggcgcgcg aggatgggcc tcgcggggag ctccgcggcc
     3541 gaggcgacga cgatggggaa gagcgcgaag ggcaagaggc ggctgacctc cttgttcggg
     3601 aaggaggtca cggcgacgag gtacaggagg gtcgccgcga gcttcgcaag cggatggagc
     3661 ccgtgcacgg ggctgtcgcc ttccgacagc ccctcgagtt cgcggatgcg caaaagggaa
     3721 tccgagacgc gggacaagcg gcctcccaag gctcaagaag aatcggcggg accccggctc
     3781 aaggaggcag gggccccgtc cgtcgactgc tagcggccgg cggcgggctg ggcagggccc
     3841 ttccgccgct tggccgcgcg gatggaccag ccgaccaggg cggcgagcgc cagggtcatg
     3901 gctccgccca caagcccggc ggcgctcgtg cccgcgctga cggccggcca ggtctcggcg
     3961 gccgcggtag ccgcgacctc ggtcgccgcg gccgcggagg ccgagggctt gaaggagtag
     4021 tcgggcatga aggcggtcgc gccctggagc tcggccagcc catcgtgcac cttgccctcg
     4081 gcctccagct cggacctgcc ggccaccttg gccatggacc actcgagtcc gtcggggttg
     4141 gcggaggcga accaggagag gatgcctccg accgcgagga cgccgacggc caggacggcg
     4201 aggaccttcc tggtcggggc ctggccgagc ccttccttcg aatcggctct ctcgaggatg
     4261 tcgggagagg ccttggcgac gtaggcgacg acggccgcgg tcacgaggcc ctccacgagg
     4321 ccgatcg
//
LOCUS       DAOOSJ010000225         4308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12898_length_4308_cov_4.541030, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000225 DAOOSJ010000000
VERSION     DAOOSJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_4308_cov_4.541030"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLG14_10245"
     CDS             complement(<1..893)
                     /locus_tag="PLG14_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOX49553.1"
                     /translation="MLAALERNPKWKGRRGPLVLVIMDGVGYGQYAEGDAVADSKMVH
                     FRAMEAAGAHTRLKAHGKAVGLPSDEDMGNSEVGHNAIGCGRVYSQGAQLVTNSLATG
                     AMFEGQTWKRLVANVRRSGGALHFIGLFSDGNVHSHIDHLKAMVARADAEGVAKVRVH
                     VLLDGRDVGEQSALEYVEPFEAFLAGLNAKGRDYRIASGGGRQYITMDRYGANWEQVR
                     RGWETHVLGKGRQFASAKAAIEAYRAEKPSVIDQDLKEFVVVEGEPGSASAKPVGAMA
                     DGDSVVYFNFRGDRALEITAA"
     gene            complement(1064..1597)
                     /locus_tag="PLG14_10250"
     CDS             complement(1064..1597)
                     /locus_tag="PLG14_10250"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOX49554.1"
                     /translation="MAEGFNLDDAIRKVPDFPKEGILFYDITSVLANPDAFRYCVDQM
                     VKLYRNEGLDAVAAVESRGFLFAAPLCDRLGIPLILVRKKGKLPGKTIGKKYELEYGQ
                     AEIEMHGADIPQGGKVLIVDDLIATGGTVRAAADLLKEGGAEPVGVFAVIGLPFLDYA
                     TTLGGLPSRTLIEYYGE"
     gene            complement(1625..2017)
                     /locus_tag="PLG14_10255"
     CDS             complement(1625..2017)
                     /locus_tag="PLG14_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49555.1"
                     /translation="MKMMKTLIVYEDGSPELERTARSIAERLDSARHEARVRAASAVT
                     IPEILASSLFFFGADAPASPSYAEIARVVAGMNLAGRKAAYYGASGAAVAWLKGIVAD
                     SDLQAAGADLVAPRPDPQALAAWLRNIA"
     gene            complement(2103..2678)
                     /locus_tag="PLG14_10260"
     CDS             complement(2103..2678)
                     /locus_tag="PLG14_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49556.1"
                     /translation="MPSPSRPAWRARRGPPLSLILAFAILSSAAQVRADEATPSAASP
                     AESAPAGAAAEAAPASAAAAPAARGSVLAAGVLPAAYGQEGKGRENALRRFEIVALGS
                     YPIMLFYTGLGFDLSAYIGSGYDPSRAPWPFKNEYSAELSDAERLQRLGTAALASLFV
                     AGLDAAIHEVRLRREERAARRLFEGRPGSPP"
     gene            complement(2684..3463)
                     /locus_tag="PLG14_10265"
     CDS             complement(2684..3463)
                     /locus_tag="PLG14_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HOX49557.1"
                     /translation="MSGYDFVADRAAGLLARAEAAAARAGRKPSEVAVMAVTKFHPIE
                     AVEAAYRAGLRLFGESRVQEAEGKFPAFLAAHPDARLEMIGTLQSNKAKRAAALFSRI
                     QSVDSAELLKELDKRARALGRRLEILLELHTGEESKAGFPDLESLFRALELHEALQGG
                     SGVAGDAAGDAAGDAAGDAAPAGLVLRGLMTMAPYTKDEAPIRASFRALREAREAALA
                     RFSLPGFDVLSMGMTNDFEAAIEEGSTLVRVGTALFGSREG"
     gene            complement(3471..>4308)
                     /locus_tag="PLG14_10270"
     CDS             complement(3471..>4308)
                     /locus_tag="PLG14_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOX49558.1"
                     /translation="ERLNRASDSAGFDAISLGGVLAWLMDCLDSGELSPEDLGVTRKP
                     RWPTASGAAAFDAVADSAHNAELGLELVDSILGKRGLLDLSEGPRKWARRIRRGKDVR
                     VLDRFVATANGRKGWMVPNQYWTPGVLAPMAIMGKYYMHYGSDFLPPRELGRACAERL
                     KKELAMDDAGLCRFHRAWGEEMMAEAIGLVYGKKEEYLEAVKVAASRINSRNAAVYWE
                     SEADLSFVRSFLVRRRDVEKDASPELARWIAAFDADPKEAALDFWFEMRKGIDESLRD
                     FL"
BASE COUNT          609 a         1461 c         1585 g          653 t
ORIGIN      
        1 aaggcggcgg tgatctccag ggcgcggtcg ccccggaagt tgaagtagac gacggagtcg
       61 ccgtcggcca tcgcgcccac gggcttcgcc gaggccgagc cgggctcgcc ctcgacgacg
      121 acgaattcct tcaggtcctg gtcgatgacg ctcggcttct ccgcgcggta ggcctcgatg
      181 gcggccttcg cgctcgcgaa ctgccggccc ttgcccagga cgtgggtctc ccagccgcgg
      241 cggacctgct cccagttcgc gccgtagcgg tccatcgtga tgtactggcg gccgccgccc
      301 gaggcgatcc ggtagtcgcg gcccttcgcg ttcagtcccg cgaggaaggc ctcgaagggc
      361 tcgacgtact cgagggcgct ctgctcgccg acgtcccggc cgtccaggag gacgtggacg
      421 cggaccttgg cgacgccctc ggcgtcggcg cgggcgacca tcgccttgag gtggtcgatg
      481 tgcgaatgga cgttgccgtc ggagaagagg ccgatgaagt ggagggcgcc gcccgaccgc
      541 ctgacgttgg cgacgaggcg cttccaggtc tgtccctcga acatggctcc ggtggcgagc
      601 gagttggtga cgagctgcgc gccctgggag tagacgcggc cgcagccgat ggcgttgtgg
      661 ccgacctcgg aattgcccat gtcctcgtcg gaggggaggc ccaccgcctt cccgtgggcc
      721 ttgaggcgcg tgtgggcgcc ggcggcctcc atggcgcgga aatggaccat cttggagtcg
      781 gcgacggcgt cgccctccgc gtactggccg tagccgacgc cgtccatgat gaccaggacc
      841 agggggccgc ggcggccctt ccacttcgga ttgcgctcaa gggctgcaag catgaggatc
      901 tccttaggct atggggtaag gccggggacg gccttggccg ggcttggacg cgcgggcccg
      961 cggagccggg gcgttgatgc gccagggcgc cggaacgccg cggccgcgga cctcgggcgg
     1021 cgtccaggca gagggaggaa ggacgaggac tggccccttc ccatcactcc ccgtaatact
     1081 cgatgagggt ccggctgggg aggccgccga gggtcgtggc gtagtcgagg aagggaaggc
     1141 cgatgacggc gaagacgccc acgggctcgg ctccgccttc cttgaggagg tcggccgccg
     1201 ccctgaccgt gccgcccgtc gcgatgaggt cgtcgacgat gaggaccttg ccgccctggg
     1261 ggatgtcggc gccgtgcatc tcgatctcgg cctggccgta ctcgagctcg tacttcttgc
     1321 cgatggtctt gccggggagc ttccccttct tcctgaccag gatgaggggg atgccgagcc
     1381 ggtcgcagag gggcgcggcg aagaggaagc ccctcgactc cacggccgcc accgcgtcca
     1441 ggccttcgtt cctgtagagc ttgaccatct ggtcgacgca gtagcggaag gcgtcgggat
     1501 tggccaggac gctcgtgatg tcgtagaaaa ggatgccctc tttgggaaaa tccggcactt
     1561 tccggatggc gtcgtcgagg ttgaagcctt cggccatggg taccttacct cttagtcgtg
     1621 gggtttaggc gatattgcgc agccaggccg cgagggcctg gggatcgggc ctgggcgcga
     1681 cgaggtcggc gccggcggcc tggaggtcgg aatcggcgac gatgcccttg agccaggcca
     1741 ccgcggcgcc cgaggcgccg tagtaggcgg ccttcctgcc ggcgaggttc atgccggcga
     1801 ccacgcgcgc gatctcggcg taggagggac tcgccggagc gtcggctccg aagaaaaaga
     1861 ggctcgaggc gaggatctcg ggtatcgtca cggccgaggc cgcgcggacg cgggcctcgt
     1921 gccgggctga gtcgagcctc tcggcgatgc tcctggcagt ccgttcgagt tcgggactgc
     1981 cgtcttcgta gacgatcagg gttttcatca tcttcattgc aaccgcgctg tcatactagt
     2041 ccgcgcccgg cgcgctgtca agttcgaccg gtcgccgcgg ccgacgggcc gctcgggagg
     2101 cttcagggag ggctcccggg gcggccctcg aagaggcgcc gcgccgcccg ctcctcgcgc
     2161 ctcagccgga cctcgtggat cgccgcgtcc aggccggcga cgaagaggga ggccagggcc
     2221 gccgtgccga gcctttggag cctctcggcg tcgctgagct cggccgagta ctcgttcttg
     2281 aagggccagg gagcgcggct cgggtcgtag cccgagccga tgtaggccga taggtcgaag
     2341 cccaggccgg tgtagaagag catgatcgga tagctgccca gggccacgat ctcgaagcgc
     2401 ctgagagcgt tctccctccc cttcccctcc tgtccgtagg cggcgggaag gacgccggcc
     2461 gccaggacgg agccccgcgc cgcgggggcc gccgcggcgc ttgcgggagc ggcctcggcc
     2521 gcggcgcccg cgggagcgga ttcggccggc gaggcggcgg atggagtcgc ctcatcggcc
     2581 cggacttgcg ccgccgagga gaggatcgcg aaggcgagga tgagggaaag cggcgggccg
     2641 cgccttgcgc gccacgcggg acgagagggg ctcggcacgg ccgctagccc tccctcgagc
     2701 cgaagagggc ggtgccgacg cgcacgaggg tggagccctc ctctatcgcc gcctcgaaat
     2761 cgttggtcat gcccatggac aggacgtcga agcccggaag ggaaaaacgc gcgagggcgg
     2821 cctccctcgc ctcgcggaga gccctgaagg aggcgcggat cggcgcctcg tccttcgtgt
     2881 agggcgccat cgtcatgagg ccccggagga cgaggccggc gggcgccgcg tcgccagccg
     2941 cgtcgccagc cgcgtcgccc gccgcgtcgc cagctacccc ggagccgccc tggagcgcct
     3001 cgtggagctc gagggcgcgg aagagggact cgaggtcggg aaagccggcc ttcgactcct
     3061 cgcccgtgtg gagctcgagg aggatctcga gccggcggcc cagggccctg gcccgcttgt
     3121 cgagctcctt gaggagctcg gccgagtcca ccgactggat cctcgaaaag agggccgccg
     3181 cccgcttcgc cttgttggac tggagggtgc ctatcatctc gaggcgcgcg tcgggatgcg
     3241 cggccaggaa ggccgggaac tttccctcgg cctcctggac cctgctctcg ccgaagaggc
     3301 gcaggccggc gcgataggcg gcctccacgg cctcgatcgg gtggaacttg gtcaccgcca
     3361 tgacggcgac ctcggagggc ttcctcccgg ccctggcggc ggcggcctcc gcgcgggcga
     3421 ggagcccggc ggcgcggtcc gcgacgaaat cgtagccgct caccgcttcc tcacaggaaa
     3481 tcccgcaggg actcgtcgat gcccttgcgc atctcgaacc agaagtcgag ggccgcctcc
     3541 ttcgggtcgg cgtcgaaggc cgctatccag cgggcgagct cggggctcgc gtccttctcc
     3601 acgtcgcggc ggcggaccag gaagctccgc acgaaggaga ggtcggcctc gctctcccag
     3661 tacacggcgg cgttgcggct gttgatccgg ctcgcggcca ccttcaccgc ctccaggtac
     3721 tcctccttct tgccgtagac gaggccgatg gcctccgcca tcatctcctc gccccaggcc
     3781 cggtggaagc ggcagagccc tgcgtcgtcc atcgcgagct ccttcttgag gcgctcggcg
     3841 caggcccgcc ccagctcgcg cggggggagg aagtcggagc cgtagtgcat gtagtacttg
     3901 cccatgatcg ccatgggcgc gaggacgccc ggggtccagt actggttcgg gaccatccag
     3961 cccttgcggc cgttggccgt cgcgacgaag cggtccagga ccctgacgtc cttcccgcgg
     4021 cggatccgcc tggcccactt cctgggcccc tcggagaggt cgagcagtcc gcgcttcccg
     4081 aggatggaat cgacgagctc gaggccgagc tccgcgttgt gcgccgagtc ggcgaccgcg
     4141 tcgaaggccg cggcgcccga cgccgtcggc caacgcggct tcctcgtgac gcccaggtcc
     4201 tccggcgaga gctcacccga gtccaggcag tccatgagcc aggccaggac gccgccaagg
     4261 gagatcgcgt cgaagcccgc cgagtcggag gcgcggttca gtcgctcc
//
LOCUS       DAOOSJ010000226         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_12935_length_4300_cov_5.391755, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000226 DAOOSJ010000000
VERSION     DAOOSJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_4300_cov_5.391755"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..>1231
                     /locus_tag="PLG14_10275"
     CDS             322..>1231
                     /locus_tag="PLG14_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOX49559.1"
                     /translation="MAISDRSAAQDQPSSPEGIPDLVESLLRSLLAGPLPKAEAEAAL
                     PLIAGARPADVVAAVDALAGEAALGGSSLEPLKPAVARLLNLLRQPLESASRGAGERR
                     DPLSLLLKAENRAALAALEALRAPVALLAAKASPPGSLPVAARELRAGLAALAPLELH
                     YARLENVLFPYFEARYPKYRCLSLMWSIHDDVRRSLRALSALLSGDKTASGASPYEPA
                     ASPLDGAAVALQSGRLFFDVNALVFREESLLFPVAIPLLSPRERLSLFGEALALGPGL
                     LGREALAELAQMAEGYAAALEDHETAA"
     assembly_gap    1232..1331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1332..1577
                     /locus_tag="PLG14_10280"
     CDS             <1332..1577
                     /locus_tag="PLG14_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOX49560.1"
                     /translation="DVRNCHPPESLGRVLGLIEDFRSGRREREAFHIQLRGRFVHIEY
                     FALRGADGEFLGVLEASEDLTEKRALSGEKRLASESP"
     gene            complement(1574..2581)
                     /locus_tag="PLG14_10285"
     CDS             complement(1574..2581)
                     /locus_tag="PLG14_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOX49561.1"
                     /translation="MRKPSVFLLVLLAAAASLGAQSLDEARKRLVAVAEGYRGAPYVY
                     GADEPPSFDCSGFVQYVYRTAISLDIPRNSKSQYAAGAAIAKEALKPGDILVFDTVGG
                     SPSHVALYLGSGSMIHAASAGPRTGVIVSALDDSYFGPRFIGARTFLGGFPGAQDAAP
                     MAAGPQAALPQAAAPQAAAAPVAAAPTAAAPKSNPQAAPAPAAPPPQAAAPAAAKPLQ
                     ARPSAAEPAVAQIGFVIPAERASYTDKIPTAKGTRLAFTLTNGTGADGGFTVIFYKTA
                     VDFSKSTEIHREFAKIRSGGSVELPPYLFSEPGIYKLIVKDSWNGQLMERIFKVIEPR
                     G"
     gene            complement(2583..2861)
                     /locus_tag="PLG14_10290"
     CDS             complement(2583..2861)
                     /locus_tag="PLG14_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49562.1"
                     /translation="MKSTEPLRALCKPGLAANRINMHPNEGFDLGLMTTRHEVEVEGL
                     GKAEVWLLNDCPKESFELNPGVWERYGKPMRAVLTFDGAKLRIEACGL"
     gene            3001..>4300
                     /locus_tag="PLG14_10295"
     CDS             3001..>4300
                     /locus_tag="PLG14_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOX49563.1"
                     /translation="MTTNLPELLLPAGGFESGIAALEGGADALYLGFSEFSARRQARN
                     FGREEYRRLLRLARSSGKKIHVAVNTVILESELEGAAGLLAFLGEFPPDALIIQDWGL
                     VALVRERFPGLAVHASTQTAAQGPGAARLALERGATRLVLPRECGLAELRLLHEALPE
                     LEYEVFVHGALCYSFSGLCLASGRLLGRSGNRGECAQVCRSYYRAESGPGMAGRTGYW
                     FSCKDLNLEADLSALVEAGAASLKVEGRMKSPEYAYAVARLYRGALDRLAGLPGAPSE
                     GELEARREEARLAFSRSPTRGYLRASSGENLLDADYPGHRGLPGAKVVAAKEGRATLE
                     LKAPLGLRDGLLSLSGPAPLEAKAFSVGELRDAATGRFLVHARAGGLVEIASPPGLAV
                     GQELERVSAREFDRRTPSPEEYPPALLALRGRLFFEAVEPE"
BASE COUNT          560 a         1586 c         1410 g          644 t
ORIGIN      
        1 tccgaggcgt tcttcgtccg cttcatgtag aagaagccga tgaggatcat cgccagcagg
       61 tagacggcga aagccgccag gacgctcaag gttcgcgaat ccatcgaatg gtcctccgcc
      121 gcataaatat gcgctggaac cgactctata tgcagaatcg catccccgtc aagccgcctt
      181 tgtcccctca tgcaagagcc tgtcccgggc gcaggccgcc ttgcgccggg cgctcgcgcc
      241 gagcatccac acagggcgct tgcgcctggc gcgggcagcg cctacgctgg acgcatccag
      301 ttaccgagag aggagccgcg gatggccatt tccgatcgaa gcgccgccca ggaccagcct
      361 tcttcgccgg agggaatccc ggacttggtc gagtccctcc tccggtccct cctggccgga
      421 cccctgccca aggccgaggc cgaggcggct ctccccctca tcgccggggc caggcccgcc
      481 gacgtcgtcg ccgccgtcga cgccctcgcg ggcgaggccg cccttggcgg ctcctccctc
      541 gagcccctga agccggccgt cgcgcgcctt ctcaacctcc tccgccagcc cttggagtcg
      601 gcctcccgcg gggccggaga gcgccgcgac cccctctccc tcctcctaaa ggccgagaac
      661 cgcgcggccc tcgccgccct ggaggccctc cgcgccccgg tggccctcct cgcggcgaag
      721 gcctccccgc ccggctccct gcctgtggcc gcgagggagc tccgcgcggg gctcgccgcc
      781 ctggctcccc tcgagctcca ctacgcccgc ctggagaacg tgctcttccc ctatttcgag
      841 gcgcgctacc cgaagtaccg ctgcctctcc ctcatgtggt ccatccacga cgacgtgagg
      901 cggagcctcc gagctctttc ggcgctcctc tcaggggaca agaccgcgtc cggcgcctcg
      961 ccttatgaac ccgccgcctc gcctttggac ggcgccgcgg tggctctcca gtcgggccgc
     1021 ctcttcttcg acgtgaacgc cctcgtcttc agggaagaga gcctcctctt tcccgtcgcc
     1081 attccgctcc tctctccccg ggaacgcctc tccctcttcg gcgaggccct ggccctgggg
     1141 ccgggcctcc ttggtcgcga agcgctcgcg gagcttgccc aaatggccga gggctacgcg
     1201 gccgctctcg aggatcacga gacggccgcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn ngacgtgcgg aactgccacc cgcccgagag cctcgggcgg gtgctcggcc
     1381 tcatcgagga cttccgctcg ggccgtcgcg agcgcgaagc cttccacatc cagctccgcg
     1441 gccgcttcgt ccacatcgag tacttcgccc tcaggggagc ggacggcgag ttcctgggag
     1501 tccttgaggc gagcgaggac ctgacggaga agcgggcgct ctcgggagag aagcgcctcg
     1561 cctcggaatc gccctagccc ctgggctcga tgaccttgaa gatcctctcc atgagctggc
     1621 cgttccagct gtccttcacg attagcttgt agatgcccgg ctcggagaag aggtagggag
     1681 ggagctcgac cgagccgccc gagcggatct tcgcgaattc ccggtggatc tccgtcgatt
     1741 tggagaagtc gacggcggtc ttgtagaaga tgacggtgaa tccgccgtcg gcgcccgtgc
     1801 cgttcgtgag cgtgaaggcc agcctcgtgc ccttggccgt cgggatcttg tcggtgtagg
     1861 aggcgcgctc cgcgggaatg acgaagccga tctgcgcgac ggccggctcg gcggcggagg
     1921 ggcgggcctg gaggggcttg gccgccgccg gggccgcggc ctggggaggc ggggcggccg
     1981 gggcgggagc cgcctggggg ttggacttcg gagcggcggc agtcggcgcg gcggccacgg
     2041 gggccgctgc cgcctggggc gcggctgcct ggggcagcgc cgcctgcgga cccgcggcca
     2101 tgggcgcggc gtcctgggct cccgggaagc cgcccaggaa ggtcctcgcc ccgatgaagc
     2161 ggggcccgaa ataggagtcg tccagggcgg agacgatgac gcccgtcctg ggcccggcgg
     2221 aggccgcgtg gatcatcgag cccgagccca ggtacagggc gacgtggctc ggcgagccgc
     2281 ccacggtgtc gaagacgagg atgtcgcccg gcttcagggc ctccttggcg atggccgcgc
     2341 ccgcggcgta ctgcgacttc gagttcctgg ggatgtcgag ggaaatggcc gtccggtaca
     2401 cgtactggac gaagcccgag cagtcgaagg aggggggctc gtcggctcca tagacgtagg
     2461 gagcgccccg atagccctcg gccacggcca cgaggcgttt ccgcgcctcg tccagggact
     2521 gggcgcccag ggaggcggcc gcggcgagga ggaccaggag gaagacggaa ggctttctca
     2581 tgctagagcc cgcaggcctc gatcctgagc ttggccccgt cgaaggtgag gacggcccgc
     2641 atgggcttgc cgtagcgctc ccacacgccg ggattgagct cgaagctctc cttgggacag
     2701 tcgttgagga gccagacctc ggccttgccc aggccctcca cctcgacctc gtggcgcgtc
     2761 gtcatgaggc ccaggtcgaa gccctcgttc gggtgcatgt tgatccggtt ggccgccagt
     2821 cccggcttgc agagcgccct gagcggctcg gtgcttttca tcgctcgcct cctcctgctc
     2881 ctcaaagcct accctccggc gccgcaaaat gccagcgccg gcgcggcgaa agctcccggg
     2941 tcccgaaggc tcagggccgc cccctagacc atgcccctcc ttcgtgctat cccgaagccc
     3001 gtgaccacca acctgcccga gctactcctc cccgccggcg gcttcgagtc cggcatcgcg
     3061 gccctggagg gcggcgccga cgccctctac ctcggcttct ccgaattctc cgcgcgccgg
     3121 caggcgagga acttcggccg cgaggaatac cgccgcctcc tccgcctggc ccgctcctcg
     3181 gggaagaaga tccacgtggc ggtcaacacc gtcatcctcg agtccgagct cgagggggcg
     3241 gccggcctcc ttgcctttct gggcgagttc ccgcccgacg ccctcatcat ccaggactgg
     3301 ggtctcgtcg ccctggtcag ggaacgcttc cccggcctcg ccgtccacgc ctcgacccag
     3361 accgccgccc agggcccggg cgcggccagg ctggccctgg aacgaggcgc gacccgcctc
     3421 gtcctccccc gcgagtgcgg cctcgccgag ctcaggctcc tccacgaggc cctgcccgag
     3481 ctcgagtacg aggtcttcgt ccacggcgcc ctctgctact ccttctcggg cctctgcctc
     3541 gcctcgggcc gcctcctcgg ccgctcggga aaccgcggcg agtgcgccca ggtctgccgc
     3601 tcctactacc gcgccgagtc cggccccggg atggccggga ggaccggcta ttggttctcc
     3661 tgcaaggacc tgaacctgga ggccgacctt tcggccctgg tcgaggccgg agccgcctcc
     3721 ctcaaggtcg aggggcggat gaagtcgccc gagtacgcct acgcggtcgc ccgcctctac
     3781 cgcggcgcgc tggaccgcct cgcgggcctg cccggcgccc cttccgaggg cgaactcgag
     3841 gccaggcgcg aggaggccag gctcgccttc tcccgctcgc cgacccgcgg ctacctcagg
     3901 gcctcctcgg gcgaaaacct cctcgacgca gactacccgg gccaccgcgg ccttccgggc
     3961 gcgaaggtcg tcgccgcgaa ggagggcagg gcgaccctcg agctcaaggc tcccctgggc
     4021 ctccgcgacg gacttctttc cctctcgggc ccggcgcccc tggaggcgaa ggccttctcc
     4081 gtgggcgagc tccgcgacgc cgcgacgggc cgcttcctgg tccacgcccg cgcaggaggc
     4141 ctcgtcgaga tagcctcgcc cccgggactg gccgtcggcc aggagctcga gcgcgtctcc
     4201 gccagggagt tcgaccgcag gacgcccagc cccgaggagt atccgccggc cctcctcgcc
     4261 ctccgcggcc gcctcttctt cgaggccgtg gagccggagg
//
LOCUS       DAOOSJ010000227         4266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13058_length_4266_cov_5.702683, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000227 DAOOSJ010000000
VERSION     DAOOSJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13058_length_4266_cov_5.702683"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLG14_10300"
     CDS             complement(<1..1520)
                     /locus_tag="PLG14_10300"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOX49564.1"
                     /translation="MRKELERLAELDRGQVLVAHIGAALGWDQETYMPPKAIEERAEQ
                     LALLEGLSHEKLSDPEIGRLLEALGSTPENPLGDASLEAEERAYLRALRRAYDQATKL
                     PADLVVELAREVSLSQAAWAEAREKNDFPAFAPHLERMVALNKRKAACLGPGKKPYDV
                     LLDLYEPGSTEASIAAVFGALRKDLVALLGKITSRPQVEDSFLRRPCEAARQEKMSEW
                     LMRLLSYDVERGRLDTTAHPFTTTLGTDDVRITTRYLEDYFPSSVFSTIHETGHALYE
                     LGIAPPPAFRRTKLHDAASMAVHESQSRLWENTIGRSLAFWKPNYAKLETLAGPALKG
                     VSVEDFCRAINKVEPSLIRTEADEVTYGLHVILRFELEAELMAGRLAVKDVPAAWNAK
                     AKELLGLTPPDDARGCLQDIHWSMGAIGYFPSYALGNLYAAQFWARMKKDIPGLEAQV
                     ESGDVAPVLGWLREKIHRHGTAYLPGELVERVTGEKLDARYFVEYLTEKYSKVYGF"
     gene            1711..3840
                     /gene="recG"
                     /locus_tag="PLG14_10305"
     CDS             1711..3840
                     /gene="recG"
                     /locus_tag="PLG14_10305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOX49565.1"
                     /translation="MFLRELNQSVTNLRGAGKAVAERLARLGVFSVADLLLRYPRDYD
                     DRSVFVPFSAFAKGGRVHTVARVTGHDWFGFGRMRTLKIEVRDASSSAILLCFNRPFL
                     EKSLPVGARVLVTGKFQFRYGEIQSSSFEAERLEDGEDRDEEDRDAGAAGGPGEGGAS
                     SLLKGILPVYALTDGLQQGNVRKLVRAALAEYGARVEDELPPSLAKGRGLLPKREALR
                     EIHFPSSPERLAAARESLVYEELFYFQLAVARRAARRREAERPSRSSPGLLKRRLLER
                     LPYSLTPDQERSAAEIAAEMAGPYPMARLLQGDVGSGKTLVAILACLDAIESGGQAAI
                     MAPTELLARQHAGTAARLLEPLGIRLAFLSGNVEDAARPPLLAALKRGEVDLVLGTHA
                     LFSDEVEYRDLRLAVVDEQHRFGVLQRLALGRKGPLPDLLMMTATPIPRTLALTVYGD
                     LSLSTIRTMPPGRKPIVTHLAKTGNDGKVYGFVRRLLAEGRQAYFVYPLIGESERLEL
                     KNAEAMYERLKDEVYPDYPAGLVHSRLKDEEKRATMDAFVRGDLRILVATSVVEVGVD
                     VPNAAAMVVEHAERFGLAALHQLRGRVGRGEAQSYCFLVFSDGLTEDAKARLKAMMET
                     NDGFALAEEDLKIRGPGELAGTAQAGALRLSIADPVRDQAVLEKARTDAFALVAADPG
                     LLGAECAVLREVLSRAKPFGDGPVGQG"
     gene            complement(3987..>4266)
                     /locus_tag="PLG14_10310"
     CDS             complement(3987..>4266)
                     /locus_tag="PLG14_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49566.1"
                     /translation="AESVLKRGAGAVEALAASEKERAKAALKAPAKAGAKAKPAAKAK
                     PKAAAKGGASAKKPAAKVAPKKSAPKKAASMAPAKKAVRGAARKPAKK"
BASE COUNT          560 a         1541 c         1523 g          642 t
ORIGIN      
        1 tagaagccat agaccttcga atacttctcc gtgaggtact cgacgaagta gcgggcgtcg
       61 agcttctcgc ccgtgacccg ctcgacgagc tcgccgggga ggtaggccgt cccgtggcgg
      121 tggatcttct cgcggagcca gcccaggacg ggggcgacgt cgcccgactc gacctgcgcc
      181 tcgaggccgg ggatgtcctt cttcatccgc gcccagaact gggcggcgta gaggttcccc
      241 agggcgtagc tcgggaagta gccgatggcg cccatcgacc agtggatgtc ctggaggcag
      301 ccgcgggcgt cgtccggcgg cgtgaggccg aggagctcct tggccttcgc gttccaggcg
      361 gcgggcacgt ccttgaccgc gaggcggccg gccatgagct cggcctcgag ctcgaagcgc
      421 aggatgacgt ggaggccgta ggtgacctcg tcggcctccg tgcggatgag ggagggctcc
      481 accttgttga tggcgcggca gaagtcctcc acggagacgc ccttgagcgc ggggccggcg
      541 agggtctcga gcttggcgta gttgggcttc cagaaggcca gggagcggcc gatcgtgttc
      601 tcccagagcc gcgactggga ctcgtggacg gccatggagg cggcgtcgtg gagcttcgtg
      661 cggcggaagg cgggcggcgg cgcgatgccg agctcgtaga gggcgtggcc cgtctcgtgg
      721 atcgtcgaga agaccgagga ggggaagtag tcctccaggt agcgcgtcgt gatgcggacg
      781 tcgtccgtgc ccagggtcgt cgtgaagggg tgggccgtcg tgtccaggcg gccgcgctcc
      841 acgtcgtagg agaggaggcg catgagccac tcgctcatct tctcctggcg cgcggcctcg
      901 caggggcgcc ggaggaagga gtcctccacc tggggcctcg acgtgatctt cccaaggagg
      961 gcgacaaggt ccttcctgag ggcgccgaag acggccgcga tggacgcctc cgtgctcccg
     1021 ggctcgtaga ggtccaggag gacgtcgtag ggcttcttgc cggggccgag gcaggcggcc
     1081 ttccgcttgt tgagcgcgac catcctctcc aggtgggggg cgaaggcggg gaagtcgttc
     1141 ttctcccgcg cctcggccca ggcggcctgg gagaggctca cctcgcgggc gagctcgacg
     1201 acgaggtcgg ccggcagctt cgtcgcctgg tcgtaggcgc ggcggagggc gcggaggtag
     1261 gccctctcct cggcctcgag ggaggcgtcg cccagtgggt tctccggcgt cgagccgagg
     1321 gcctcgagga ggcggccgat ctcgggatcg gagagcttct cgtgggagag gccttcgagg
     1381 agggcgagct gctcggcccg ctcctcgatg gccttcgggg gcatataggt ttcctggtcc
     1441 cagccgaggg ccgccccgat gtgggcgacg aggacctgcc cgcggtcgag ctccgcgagg
     1501 cgttcgagtt ccttccgcat gttcgattct ccttgtttag gcgcgtcgcc cgatgcgccg
     1561 gcggcgcctg agggacatcc tagcacccct ccccgcggag ggcaaggaag aaggcggggc
     1621 ctaggccgcg gggcctcgcg cgggcggagg cggccgcgcc cgggccgcgg ggcctcgcgc
     1681 gggcggaggc ggcgtgctat cctcgaaggc gtgttcctcc gcgagctcaa ccagtccgtg
     1741 accaacctca ggggcgcggg caaggccgtg gccgagcgcc tcgcgaggct cggcgtcttc
     1801 tcggtcgccg acctcctcct ccgctatccg cgcgactacg acgaccgctc cgtcttcgtc
     1861 cccttctccg ccttcgccaa gggcgggcgg gtccacaccg tcgccagggt gacgggccac
     1921 gactggttcg gcttcggccg catgcgcacc ctgaagatcg aggtgcgcga cgcctcctcc
     1981 tccgccatcc tcctctgctt caaccggccc ttcctggaga agagcctccc cgtcggcgcc
     2041 cgggtcctcg tcacgggcaa gttccagttc cgttacggcg agatccagtc ctcctccttc
     2101 gaggccgagc ggctcgagga cggggaagac cgggacgagg aggaccggga cgcgggcgcg
     2161 gccggagggc ccggggaggg cggcgcctcc tccctcctca agggcatcct cccggtctac
     2221 gccctcacgg acggcctcca gcagggcaac gtgcgcaagc tcgtccgcgc ggccctggcc
     2281 gagtacggag cccgggtcga ggacgagctg ccgccctccc tcgccaaggg ccgcggcctc
     2341 cttcccaagc gagaggcgct gcgcgagatc cactttccct cctcgcccga gcgcctggcc
     2401 gcggcccgcg agagcctcgt ctacgaggag ctcttctact tccagctcgc ggtcgcgagg
     2461 cgggccgcca ggcgccggga ggccgagcgg ccttcccgct cctcgccggg cctcctcaag
     2521 cgccgcctcc tcgagcgcct cccctactcc ctcacgccgg accaggagcg ctcggccgcg
     2581 gagatcgccg cggagatggc cggcccctac cctatggcgc ggctcctcca gggcgacgtc
     2641 ggctcgggga agaccctggt cgccatcctg gcctgcctgg acgccatcga gtccggaggc
     2701 caagccgcga tcatggcccc gaccgagctc ctcgcgaggc agcacgcggg caccgcggcc
     2761 cgcctcctcg agcccctggg gatccgcctg gccttccttt cgggcaacgt ggaggacgcg
     2821 gcccgccctc ctctcctcgc cgccctgaag cggggggagg tcgacctcgt cctcggcacc
     2881 cacgccctct tctccgacga ggtcgagtac cgcgacctcc gcctggccgt cgtcgacgag
     2941 cagcaccgct tcggcgtcct ccagcgcctg gccctggggc ggaagggccc cctccccgac
     3001 ctcctcatga tgacggcgac gcccatcccg cggaccctcg ccctcaccgt ctacggcgac
     3061 ctctccctct ccacgatcag gaccatgccg ccggggcgga agcccatagt cacccacctc
     3121 gccaagacgg gcaacgacgg gaaggtgtac ggcttcgtcc gccgcctcct cgccgagggg
     3181 cggcaggcct atttcgtcta tcctctcatc ggggagtcgg agcggctgga gctcaagaac
     3241 gcggaggcca tgtacgagcg gctcaaggac gaggtctacc cggactaccc ggccggcctc
     3301 gtccactcgc ggctcaagga cgaggagaag cgggcgacga tggacgcctt cgtccgcggc
     3361 gatctcagga tcctcgtcgc gaccagcgtc gtcgaggtcg gcgtcgacgt cccgaacgcc
     3421 gcggcgatgg tcgtggagca cgcggagcgc ttcggcctcg ccgccctcca ccagctgcgc
     3481 ggccgcgtcg gccgcggcga ggcccagtcc tactgcttcc tcgtcttctc cgacggcctc
     3541 acggaggacg cgaaggcgag gctcaaggcg atgatggaga cgaacgacgg cttcgccctg
     3601 gcggaggagg acctgaagat ccgcggcccc ggcgagctcg cgggcacggc ccaggccgga
     3661 gccctgcggc tctccatagc cgaccccgtc cgcgaccagg ctgtcctgga gaaggcccgg
     3721 accgacgcct tcgccctcgt cgccgccgat ccgggcctcc tcggggcgga atgcgccgtc
     3781 ctccgggagg tcctctcccg cgcgaagccc ttcggcgacg gcccggtcgg ccaaggctga
     3841 gcgaccgggc cggggaccgg tcggccaggg atgatggacc gcgccgaaag gccgacctcg
     3901 ccgcccctgg accaaaaaag accgcccccc gccctggccg ggcggagaac ggtccttgac
     3961 gcgcgggcct tctagaaaag gagtgtctat ttcttggccg gtttcctggc ggcgccgcgg
     4021 acggccttct tggccggggc catggacgcg gccttcttgg gcgcggactt cttgggcgcg
     4081 accttcgccg ccggcttctt ggcagaggcc ccgcccttcg cggccgcctt gggcttggcc
     4141 ttcgccgcag gcttcgcctt cgcgccggcc ttggccggcg ccttcagggc ggccttggcg
     4201 cgctccttct ccgaggcggc cagggcctcc accgcgccgg cgccgcgctt gagcaccgac
     4261 tcggcc
//
LOCUS       DAOOSJ010000228         4262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13078_length_4262_cov_4.939149, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000228 DAOOSJ010000000
VERSION     DAOOSJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_4262_cov_4.939149"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..2073)
                     /locus_tag="PLG14_10315"
     CDS             complement(310..2073)
                     /locus_tag="PLG14_10315"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOX49567.1"
                     /translation="MSVEKLKPVPSDIEIAQAAKIRPIVEIAERYGICDECIDHFGRY
                     KAKVRLEFMKDNADKPNRAKYIDVTAITPTPLGEGKTTTTVGLAQGLGVLGRKAIAAI
                     RQPSMGPTFGIKGGAAGGGYSQIVPMADFNLHLTGDIHAVSAAHNLCSTALDARIYHE
                     GRWSDAYFEKLGMRKLNMDPYEVWWRRVVDMNDRTLRRIVVGVGGIDNGPLRETGFDI
                     AVASEVMAILALAKDLRDLRERLGRIVLGLDKAGKPVTTEDLGVAGAMTILLKDAIKP
                     NILQTLEEQLAFVHAGPFANIAHGNSSVIADLMAMKIGDYVVTESGFGSDMGLEKFVD
                     IKCRTSGLWPDAAVVVATVRALKMHGGGPKVTPGKALATAYTQEDRELVRKGLSNLEA
                     HLRIVRKIGIPAVVAINAFPTDSEAEQELVREAAIKAGAVDAVVTRNWAEGGAGAAEL
                     AAAVEKAAAQPSNCGPFYPLDLTIKEKIERIAREIYGAEHVAYSAEAEGRIETFEKLG
                     YGNLPICMAKTQYSISHDPSLKGAPKGFIFPVNDLRVAAGAGFVYPISGEINTMPGLP
                     SKPGYMGMDIDPDTGRITGLS"
     gene            2320..2745
                     /locus_tag="PLG14_10320"
     CDS             2320..2745
                     /locus_tag="PLG14_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49568.1"
                     /translation="MRKTTALFAALALCAAAAFGQALSFGLGDAGLEASLNELGASAK
                     LDLPGFTAEVSLQWGVPQAQVQATLAQGLAPAEAYLAAALASISKKPLDAVVAAYKKD
                     KAAGWGALAKSLGVKPGSKEFKALKDKTQASAAKAKKKK"
     gene            complement(2942..4093)
                     /locus_tag="PLG14_10325"
     CDS             complement(2942..4093)
                     /locus_tag="PLG14_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOX49569.1"
                     /translation="MQYRRFPKIDGLEVSTLGLGCMRLPQRSADPADIDEAALDAMVL
                     AAREAGVNYLDTAYIYHKGRSEAALGESLERTGSRGDFLLATKSPVWLAKDSGDWERF
                     LDEQLKRLKTDHVDFYLLHALSAERWALVEKLGGLRAMERFRMDGRIRHLGFSFHDNL
                     DAFKRIVDGYPAWEFCQVQYNYVDKDFQAGEAGLRYAAEREIGAIVMEPLRGGALVRA
                     PRAVREIFARHPKPRMLAEWAMRFVWERQEVTTALSGMGSVDQVLENAAYAEAARMNS
                     LTRAEMALFDEARAFFKEREKVPCTGCGYCRPCPSGVSIPDCFELYNAAAMFDIKADR
                     GRWYEAAYRSQGKGGNACVQCGACVPKCPQGIAIPERLAEADGYLKGGA"
BASE COUNT          597 a         1532 c         1483 g          650 t
ORIGIN      
        1 tacgggaagg ccctggaggc ggcgaaaaag gccctcgcgg ccggcgcggg ctactaggcg
       61 gggccaaagg cccggcggcg ccgcgctgcg gtgccgccga gccccgcgct ctgcgccccg
      121 cgccgaagcc gggccgcgcg ggcccgccgc gttccgcgcg gaggcgaggc gcgcgcggta
      181 gcccgaccgc aaaacccatc cgcgcgcggc atcggccgcc cgcagcaaga aggaaggccc
      241 ggggatccct ccccgagcct tcctcgcgtc ctggccccgg cctccaggtc ggccgatggg
      301 ctctcctcct caggaaagac ccgtgatcct ccccgtgtcc gggtcgatgt ccatgcccat
      361 gtagccgggc ttcgagggga gacccggcat cgtgttgatc tcgccggaga tcgggtagac
      421 gaagccggcg cccgcggcca cccgcaggtc gttcaccggg aagatgaagc ccttgggcgc
      481 gcccttcagg ctcgggtcgt gggagatcga gtactgggtc ttggccatgc agatggggag
      541 gttgccgtag ccgagcttct cgaaggtctc gatcctgccc tcggcctcgg cggagtaggc
      601 gacgtgctcg gcgccgtaga tctcgcgggc gatccgctcg atcttctcct tgatggtgag
      661 gtcgagggga tagaagggcc cgcaattcga gggctgggcc gcggccttct ccacggcggc
      721 cgcgagctcg gccgcgcctg ccccgccctc ggcccagttc cgcgtgacca cggcgtccac
      781 ggctccggcc ttgatcgcgg cctcgcggac gagctcctgc tcggcctcgc tgtccgtcgg
      841 gaaggcgttg atcgcgacga cggcggggat gccgatcttc ctgacgatgc gcaggtgggc
      901 ctcgaggttc gagagcccct tcctgacgag ctcgcggtcc tcctgggtgt aggcggtcgc
      961 tagggccttg cccggcgtca ccttggggcc gccgccgtgc atcttcaggg cccgcaccgt
     1021 cgcgacgacg acggccgcgt cgggccagag ccccgaggtg cggcacttga tgtccacgaa
     1081 cttctcaagg cccatgtcgg agccgaagcc cgactccgtg acgacgtagt cgccgatctt
     1141 catcgccatg aggtcggcga tcaccgagga gttgccgtgg gcgatgttcg cgaagggccc
     1201 ggcgtggacg aaggcgagct gctcctccag ggtctggagg atattcggct tgatcgcgtc
     1261 cttgaggagg atcgtcatcg cgccagcgac gcccaggtcc tccgtcgtca cgggcttccc
     1321 cgccttgtcc aggcccagga cgatccgccc gagccgctcg cggaggtcgc ggaggtcctt
     1381 ggccagggcc aggatggcca tgacctcgct cgcgacggcg atgtcgaagc cggtctcgcg
     1441 cagggggccg ttgtcgatgc cgcccacgcc gacgacgatg cggcgcaggg tcctgtcgtt
     1501 catgtccacg acgcggcgcc accagacctc gtaggggtcc atgttgagct tgcgcatccc
     1561 gagcttctcg aagtaggcgt cggaccagcg gccctcgtgg tagatcctag cgtccagggc
     1621 ggtggagcag aggttgtgcg ccgcggagac ggcgtggatg tcgcccgtga ggtggaggtt
     1681 gaagtccgcc atcggcacga tctggctgta gccgcctcct gccgccccgc ccttgatccc
     1741 gaaggtcggc cccatggagg gctggcggat cgcggcgatc gccttccgcc ccaggacccc
     1801 gagcccctgg gccaggccga ccgtcgtcgt agtcttgccc tccccgaggg gcgtcggcgt
     1861 gatggccgtg acgtcgatgt acttcgcgcg gttgggcttg tccgcgttgt ccttcatgaa
     1921 ctcgagccgc accttggcct tgtagcggcc gaaatggtcg atgcactcgt cgcagatgcc
     1981 gtagcgctcg gctatctcga cgatgggacg tatcttcgcc gcctgggcga tctcgatgtc
     2041 gctcggcacc ggcttcagct tctctacgga catctcctgc ctcctcgcgt ataccctacc
     2101 tacaagctaa ggcctggggg gcggagagtc aacggaagcg ggccggcgga aggccggggg
     2161 cgggccgggg agggcgagtg ccgggcggcg cgctgccccg cggcggacag ccggcggctc
     2221 gctcgcgctc ccgtggcttg atggccgcac gctcgcccgc cgcacgcttg cgcgcgaggc
     2281 ttccgaaggt cgacaatgaa ggcgcggaaa ggagaaacca tgagaaagac gacagccctc
     2341 ttcgccgccc tcgccctctg cgcggccgcg gccttcggcc aagccctctc cttcggcctc
     2401 ggcgacgccg gactggaggc gagcctcaac gaactcggcg cgagcgccaa gctcgacctc
     2461 ccgggcttca cggccgaggt cagcctccag tggggcgtcc cccaggccca agtccaggcg
     2521 accctggccc agggcctcgc ccccgcggaa gcctacctcg cggccgccct cgcctcgatc
     2581 tccaagaagc ccctcgacgc ggtcgtcgcg gcctacaaga aggacaaggc ggcgggctgg
     2641 ggcgccctcg cgaagagcct cggcgtcaag cctggctcca aggagttcaa ggcgctcaag
     2701 gacaagacgc aggcctcggc cgccaaagcc aagaaaaaga agtagcggcc agatcggcct
     2761 tcgcgggact cgcctcggat cggccatcgc gcgagcgcgg gccgcgcccg gggccgccgc
     2821 gaggcgcgcc ggcgccggga ctctcctgcg gcctcgccgc gtcgcgcgcc ggggcggcgc
     2881 gccgcagggc gtccccgcgg gtccgtcggc ggccgccgaa agccggccgc cccgccctcc
     2941 ctcaggcccc gcccttcagg taaccgtccg cctcggccag cctctcgggg atggcgatgc
     3001 cctgcgggca cttcgggacg caggccccgc actggacgca ggcgttcccg cccttcccct
     3061 gcgagcggta ggccgcctcg taccagcggc cccggtcggc cttgatgtcg aacatcgcgg
     3121 ccgcgttgta gagctcgaag cagtccggga tggagacccc gctcggacag ggcctgcagt
     3181 agccgcagcc ggtgcagggc accttctccc tctccttgaa gaaggccctc gcctcgtcga
     3241 agagcgccat ctccgcccgg gtgagcgagt tcatcctcgc cgcctccgcg taggccgcgt
     3301 tctccaggac ctggtccacg ctccccatgc cggagagcgc cgtcgtcacc tcctgccgct
     3361 cccagacgaa gcgcatcgcc cactcggcga gcatccgcgg cttcgggtgg cgggcgaaga
     3421 tctcgcgcac cgcgcgcggc gcccggacca gggccccgcc gcgcaggggc tccatgacga
     3481 tggcgcctat ctcgcgctcg gccgcgtagc ggagccccgc ctcgccggcc tggaagtcct
     3541 tgtcgacgta gttgtactgg acctggcaga actcccaggc gggatagccg tcgacgatcc
     3601 gcttgaaggc gtccaggttg tcgtggaagg agaagcccag gtgcctgatc cgcccgtcca
     3661 tcctgaagcg ctccatcgcc ctgagcccgc ccagcttctc gaccagggcc cagcgctcgg
     3721 ccgacagggc gtggaggagg tagaagtcga cgtggtcggt cttgagccgc ttaagctgct
     3781 cgtccaggaa gcgctcccag tcgcctgagt ccttcgcgag ccagaccggg ctcttcgtcg
     3841 cgaggaggaa gtcgccccgg ctgcccgtcc gctccagcga ctcgcccagg gccgcctcgc
     3901 tccggccctt gtggtagatg taggccgtgt ccaggtagtt cacgcccgcc tcgcgcgcgg
     3961 ccaggaccat cgcgtccagc gcggcctcgt cgatgtcggc gggatcggcg ctcctctgcg
     4021 ggagcctcat gcaaccgagc ccgagcgtgg agacctcgag cccgtcaatc ttggggaagc
     4081 gtcggtactg catgggccga tgatagcgcg aagagggcga aagggggagt acgtgccgtc
     4141 ggatgcccgg atcgccccgc cgcaggcctg gcggggggcc cgcttcgggg gcgcagggcc
     4201 ctcgctacgc cgtgtcaagc gacatccaga attgaccccc gggcgacatt cagttttgac
     4261 cc
//
LOCUS       DAOOSJ010000229         4257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13098_length_4257_cov_5.594955, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000229 DAOOSJ010000000
VERSION     DAOOSJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_4257_cov_5.594955"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="PLG14_10330"
     CDS             complement(<1..2027)
                     /locus_tag="PLG14_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOX49570.1"
                     /translation="MMDSQNSGKPWENPELVARNRLPMRSPLLPFPRAEAALADALAG
                     PRGRAGNPGSLPAGNPWLLSLDGRWRFALAPSPEAAPAGWDGPACDDSGWAEIEVPGS
                     WSLQGFDKPHYTNVVMPFEGAPPASPAVNPTGLYRLAFDLPAAWKGRRVVLRVGSAES
                     FLSVRANGMEVGFSKDSRLPAEFDLGPFLREGRNLLALKVIRYSDSSYIEDQDQWWLG
                     GLHRGVYLYSTEGAYLRDLDARPTLSADFSSGSVELAASLGFSATPAPGSPSWRVRAR
                     LYGPIATFSGATAHGAKVRGAADRGPKPLAEAEAEVGPSYRASGWEARLSLPVASPEH
                     WSAESPSLYVLVATLLDPEGREVESEACRLGFRRVEVRGGALLVNGKRVLVKGANRHE
                     HDERRGKTLSVESMVRDIEILKRHNFNAVRNSHYPNDERWYELCDEYGLYLFDEADIE
                     SHAYYDRLCRDPRWLLAFQDRVSRMALRDKNHASVIVWSLGNESGYGPNHDAAAAWLR
                     SFDPTRPLHYEGACRPDWGQGPHPLDTAGRGRAATDIVSPMYPSVAYLEEWDRLGADD
                     RPFIMCEFSHAMGNSNGGLSDYWELVERSRSLQGGFVWELMDHGLLVGEGGADLPSSL
                     VPAGPNAAPSGSPAGGKAWRYGGDFGDSPSDLDFIADGLLFPDRGPKPAMA"
     gene            complement(2038..3165)
                     /locus_tag="PLG14_10335"
     CDS             complement(2038..3165)
                     /locus_tag="PLG14_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX49571.1"
                     /translation="MSDEDVPTSKNARIYAWLREEFDAERIAVGARLPSENELCERFG
                     ASRPAARQALARLAHEGLVRTVRGSGSFRAPPPRSTSRDAALVLPALSTYIYPELVEA
                     ASSAFRSLGFQTLIDCTSSDMATERRVLESLRARRPACAAVSPLKGLFPGEEGAESQA
                     TSLELLKDLRAAGTEVLLLDNELGSGLFSSIVLDDRLGGERAVERLWSLGHRDIAVVR
                     WRGHAPFGARREGALAALASLGAAAGPEADLDAGSVYAGREERRAALRAALDARAALG
                     DLPTAYFCSNDELAFLAAELLSERGIRAGEDFSLIGFDDSPLARTAGLTTFTYPSRWI
                     GERAAGLLAEAVGAGRPRSRVRVSLEPELVERSSVRDLRIR"
     gene            3341..>4257
                     /locus_tag="PLG14_10340"
     CDS             3341..>4257
                     /locus_tag="PLG14_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX49572.1"
                     /translation="MRIEDIAREAGVSTATVSRVLNNPELVKRETRDRVLAVVRRREY
                     TPNSLARGLMTKQSKTVGVLIISISNSYYMEITETIQRRLREAGFMMLLAATDDRAEL
                     ERAYIREFGSRRADGIVVIDASEENFADGFFEREAAKRPLVLVHSSPRIASSPLGQVF
                     VDQRLGMRGVMDYLAGLGHAEVALLRGERGFSYDVKEAARAEWLAERGLPERRDLVLR
                     IEDGNSEDAIPLAEEAVGAAIREGRLPTAVFACNDLMARGAVNAATRAGLRIPEDLSI
                     VGHDNTILAVSGRVQLTSVDLKMRSVGQAA"
BASE COUNT          638 a         1443 c         1601 g          575 t
ORIGIN      
        1 tcggccatcg cgggcttggg gccgcggtcg gggaagagga ggccgtcggc gatgaagtcg
       61 aggtcggagg gcgagtcgcc gaagtcgcct ccgtagcgcc aggccttgcc gcccgcgggc
      121 gagccggacg gagccgcgtt gggccccgcg gggaccaagg aggagggaag gtcggcgccg
      181 ccctccccca cgaggaggcc gtggtccatg agctcccaga cgaagccgcc ctggagggag
      241 cggctcctct ccacgagctc ccagtagtcg gagaggccgc cgttcgagtt gcccatcgcg
      301 tgggagaact cgcacatgat gaagggccgg tcgtcggcgc cgagcctgtc ccattcctcg
      361 agataggcga cgctggggta catgggcgag acgatgtccg tcgcggccct tcccctgccg
      421 gccgtgtcga ggggatgggg gccctgcccc cagtcgggcc ggcaggctcc ctcgtagtgg
      481 agggggcggg tgggatcgaa ggagcggagc caggcggccg cggcgtcgtg gttcggcccg
      541 tagcccgact cgttgcccag ggaccagacg atgaccgaag cgtggttctt gtcgcgcagc
      601 gccatgcggg agacgcggtc ctggaaggcg aggagccatc gcgggtcgcg gcagagccgg
      661 tcgtagtagg cgtggctctc gatgtccgcc tcgtcgaaga ggtagaggcc gtactcgtcg
      721 cagagctcgt accagcgctc gtcgttcgga tagtgcgagt tgcggacggc gttgaagttg
      781 tggcgcttga ggatctcgat gtcgcggacc atcgactcga cggagagggt cttgcccctc
      841 ctctcgtcgt gctcgtggcg gttcgcgccc ttcacgagga cgcgcttgcc gttcacgagg
      901 agggctccgc cccggacctc aacgcggcgg aagccgaggc ggcaggcctc gctctcgacc
      961 tcccgcccct cggggtcgag gagggtggcg accaggacgt agaggctcgg gctctcggcg
     1021 ctccagtgct cgggcgaggc gacggggagg gagagcctgg cctcccagcc cgaggcgcgg
     1081 taggaggggc cgacctcggc ctcggcctcc gccaggggct tcggaccgcg gtccgccgcg
     1141 ccgcggacct tcgcgccgtg ggccgtcgcg ccgctgaacg tcgcgatggg gccgtagagc
     1201 cgcgcgcgga cgcgccagga agggctcccg ggcgcgggcg tggcggagaa gccaagggag
     1261 gcggcaagct cgaccgagcc cgaggagaag tcggcggaga gcgtgggacg ggcgtccagg
     1321 tcgcggaggt aggcgccttc ggtggagtag aggtagacgc cgcggtggag gccgccgagc
     1381 caccactggt cctggtcctc gatgtagctc gagtccgaat agcggatgac cttgagggcg
     1441 aggaggttcc ttccctccct gaggaaggga ccgagatcga actccgccgg aaggcgggag
     1501 tccttggaga agccgacctc catcccgttc gcgcggacgg agaggaagct ctccgcgctc
     1561 ccgacccgca ggacgacgcg gcgccccttc caggccgcgg gcaggtcgaa ggcgaggcgg
     1621 tacaggcccg tggggttcac ggcgggcgag gccggcggcg cgccctcgaa gggcatgacc
     1681 acgttcgtgt agtgcggctt gtcgaagccc tggagggacc aggagccggg cacctcgatc
     1741 tccgcccagc ccgagtcgtc gcaggcgggg ccgtcccagc ccgctggcgc cgcctcgggc
     1801 gagggggcga gggcgaagcg ccagcggccg tccagggaga ggagccaggg attcccggcg
     1861 ggcagggagc ccggattgcc cgccctgccc ctggggccgg ccagggcgtc ggccagggcc
     1921 gcttccgcgc gggggaaggg aaggaggggg ctccgcatcg ggaggcggtt cctggcgacg
     1981 agctcggggt tctcccaggg ctttccggaa ttctggctgt ccatcatcgc ggtctcctta
     2041 tcgtatgcgc aggtcgcgga ccgagctccg ctcgacgagc tcgggctcca gggaaacgcg
     2101 gacgcgcgag cggggccgcc cggcgccgac cgcctcggcc aggagcccgg ccgcccgctc
     2161 gccgatccat cgggaggggt atgtgaaggt cgtgaggccc gccgtccgcg ccaggggcga
     2221 gtcgtcgaag cctatgaggg agaagtcctc tccggcgcgg atgccgcgct cggagaggag
     2281 ctccgccgcg aggaaggcga gttcgtcgtt ggagcagaaa taggccgtgg ggagatcccc
     2341 cagcgcggcg cgcgcgtcga gggctgcccg gagcgccgcc cgcctctcct cccggcccgc
     2401 gtagacggag cccgcgtcca ggtcggcctc tggcccggcc gccgcgccca gcgaggccag
     2461 ggccgcgagg gcgccttccc gcctcgcgcc gaagggagcg tgccccctcc acctcacgac
     2521 ggctatgtcc cggtgcccga gcgaccagag gcgctcgacg gcccgctcgc cgcccagcct
     2581 gtcatccagg acgatggagc tgaagagccc ggagccgagc tcgttgtcga ggaggaggac
     2641 ctccgtcccg gccgcgcgga ggtccttgag gagttcgagg ctcgtcgcct gggactcggc
     2701 gccctcctcg cccgggaaga ggcctttgag gggcgagacc gcggcgcagg cgggccgcct
     2761 ggcgcgcagg gactcgagaa cgcggcgctc ggtcgccatg tccgaggagg tgcagtcgat
     2821 gagggtctgg aagcccaggc tccggaaggc cgaggaggcc gcctccacga gctcggggta
     2881 gatgtaggtg gagagggcgg gaaggacgag ggccgcgtcc cgggaggtac tgcggggagg
     2941 aggggcgcgg aaggagcccg agccgcggac cgtccgcacg agtccctcgt gggccaggcg
     3001 ggccagggct tgcctggccg cgggccggga ggcgccgaag cgctcgcaga gctcgttctc
     3061 cgagggcagt ctggccccta cggctatccg ctcggcgtcg aattcctccc ggagccaggc
     3121 gtagatccgg gcgtttttcg atgtaggtac atcttcatcc gacatgtact acatgttagc
     3181 cgacagggaa aggccggtca aggccggctt cctcgatcgg ttcccgcgat cggttcccgc
     3241 caccggctac cgccgcagac cccgcaagca ggcgggaggg attcgccttg acaccgaagc
     3301 ggaatggatg cacattgtaa gcgcttacat aagaggaacc atgcgcatcg aagacatagc
     3361 ccgggaagcg ggggtctcca ccgccaccgt ctccagggtg ctcaacaatc ccgaactcgt
     3421 caagcgggaa acgagggacc gggtcctcgc ggtggtccgc aggcgcgaat acacccccaa
     3481 ctccctcgcc cgcggactca tgaccaagca gtccaagacc gtcggcgtcc tcatcatctc
     3541 gatctccaac tcctactaca tggagatcac ggagaccatc cagcgccggc tccgcgaggc
     3601 gggcttcatg atgctcctcg cggcgacgga cgacagggcc gagctcgagc gcgcctacat
     3661 ccgcgagttc ggctcccgcc gcgccgacgg catcgtcgtc atcgacgcct ccgaggagaa
     3721 cttcgcggac ggcttcttcg agcgcgaggc cgccaagcgc cccctcgtcc tggtccactc
     3781 gagtccgcgc atcgcctcct cccccctggg ccaggtcttc gtcgaccagc gcctgggcat
     3841 gcggggggtc atggactacc tcgcgggcct gggccacgcc gaggtcgccc tcctccgcgg
     3901 cgagcgcggc ttctcctacg acgtcaagga ggcggcgcgg gccgaatggc tcgccgagcg
     3961 cggccttccc gagcgccgcg acctcgtctt gcggatcgag gacggcaaca gcgaggacgc
     4021 catccccctc gccgaggagg cggtgggcgc ggcgatccgc gagggcaggc tgccgacggc
     4081 cgtcttcgcc tgcaacgacc tcatggccag gggcgcggtc aacgcggcca cgcgggccgg
     4141 cctccgcatc cccgaggacc tctccatagt cggccacgac aacaccatcc tcgccgtctc
     4201 gggccgcgtc cagctcacct cggtggacct gaagatgcgg agcgtgggcc aggccgc
//
LOCUS       DAOOSJ010000230         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13365_length_4196_cov_4.846414, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000230 DAOOSJ010000000
VERSION     DAOOSJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13365_length_4196_cov_4.846414"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1814)
                     /locus_tag="PLG14_10345"
     CDS             complement(270..1814)
                     /locus_tag="PLG14_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOX49573.1"
                     /translation="MTVAAHEPSGFEGVLVLVEVDIRRGIPQVDLVGLAEGAVREARE
                     RVRAAVRNAGFEFPQDRVLVSLVPADLPKEGSAYDLPIALAVLGAAGLLPDPGRPFLA
                     LGELGLEGSVRPVRGVLPAVAAGLKAGIEGFIVPRANVEEAMAVGRGVIHGIGALSEA
                     AETLVRLREGRDPEPRRPREAQPAQGAEAGDLSEIRGLGRARRALEIAAAGGHHLLLF
                     GPPGSGKTMVARRLPTILPDLREEEAVAVAKIQSLAGLLGADAGLPRRPPFRAPHHSS
                     SLEGLVGGGGRLGPGEASLAHRGVLFVDEAPEFHRDALQALREPLEEGAVSLARAGRT
                     ARLPADFQLVMAANSCPCGNLGREGRACVCSPQEIQRYWRRLGGPLLDRIDLRVPLRP
                     VDARELLGPAGETSDAVRLRVAAARSLQSRRYEGFGFDRNGRIPAGLIERFCALDAEA
                     SRALAVAVEKLSLSSRACHSVLRIARTIADLSDPPGAVAIGEAALLEAVQHRRYGDGD
                     FFWKEA"
     gene            complement(2053..2352)
                     /locus_tag="PLG14_10350"
     CDS             complement(2053..2352)
                     /locus_tag="PLG14_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49574.1"
                     /translation="MNAGLAVYLVSRLAVAATTAFLAIVLWSRTRDLAWMLIVIGAIA
                     SYADILFDLLVRFGLLDEARLSPFGLPLGRIVFSNLPYLFLSAAFLVMILRRRTR"
     gene            2436..3278
                     /locus_tag="PLG14_10355"
     CDS             2436..3278
                     /locus_tag="PLG14_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49575.1"
                     /translation="MDLWYSRPESPGSDFRVKVRRPLFSAESGERRLDVLETEDFGRL
                     LLVDGRIVLAENDGAAYREMAVHVPLNAHPGVRSVLVAGGGDGSVVAELVRYPEVERI
                     VVAEPDDGVVEAARRWFPGIAASLRDPRVRLVAEEAAGFARETKERFDLVIVDRPDDE
                     GKAELGQAFYCDCFRILSGDGILVNRAGSATFPGRRRDLVRSAGRLKRLFPVYRLYRV
                     SPPAGEPGELLLGFASKRYEPSKGPDEEAWEKRGIATSYYNAALHCAAFALPQYAQEL
                     LEGV"
     gene            complement(3393..>4196)
                     /locus_tag="PLG14_10360"
     CDS             complement(3393..>4196)
                     /locus_tag="PLG14_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX49576.1"
                     /translation="GLPLGRLLFRPVREKAGLGTVRLAVSGGGPLAPATADFFDALGI
                     NLVQGYGMSENGPLISVNLPKRKDNRSVGFPVKHTELRIGEPGPDGIGEIQVRSPSLM
                     EGYLDNPEASREALTEDGWLKTGDLGRVDARGFVFITGRRKSLIVTEGGKNVYPEEIE
                     GRFEGSAWIKESLVVGRPSRRGHPGEEVIAVLVPDFEKLALAHPGREKDAAFVESLLR
                     EEVQRVNKTLPPYMKLADFLVKDEEFEKTSTRKIKRFIYQEYAKANPRR"
BASE COUNT          622 a         1460 c         1503 g          611 t
ORIGIN      
        1 ccagaaggcg ggcattttgt agcactttcg tgcccgctct gtcaagcgct caggcggaaa
       61 tcttttttga agattttcgc cgccggaatc gagggctttt caggccactc ggtccacaag
      121 gcgctcgatc aggctactac cgtagccgcg ttcgtgtcaa gcgccgcgct ctcgtaccca
      181 tcgcttttta tgttcgcgcc gcctgttcgc cggcgcggat gaataaatac ccgatcggca
      241 ttttttagtc aatcgtcccg cgccggaaat cacgcttcct tccagaaaaa atcgccgtcg
      301 ccgtagcgcc ggtgctggac ggcctccaac agcgccgcct cgccgatggc gacggcgcca
      361 ggagggtccg agaggtccgc tatcgtcctc gctatgcgca agacggaatg gcaggcccgg
      421 gaggagaggg acagcttctc gaccgcgacg gccagggccc gggaagcctc cgcgtccaag
      481 gcgcagaagc gctctatgag ccccgcggga atccgaccgt tccggtcgaa accgaaaccc
      541 tcgtagcgcc tggattggag gctccgcgcg gcggccaccc gcagcctcac ggcatcggag
      601 gtctcgccgg cggggcccag gagttccctc gcgtcgacgg gccgcaaggg aacccgcagg
      661 tcgatcctgt cgaggagggg gccgcccagt cttctccagt agcgctggat ctcctggggg
      721 gagcagacgc aggccctgcc ctccctgccc aggttgccgc aggggcagga gttggccgcc
      781 atgacgagct ggaagtccgc cggcagcctc gcggtcctgc ccgcccgggc gagggagacc
      841 gcgccctcct caagcggctc cctgagggcc tgcagggcgt cccgatggaa ctcgggagcc
      901 tcgtcgacga agagcacgcc cctgtgggcg aggctggcct cgccggggcc cagcctccct
      961 cccccgccga ccaagccttc gagggatgag gaatggtgcg gggcgcggaa gggaggccgc
     1021 ctgggaagtc ccgcgtcggc gcccaggagg ccggccaggg actggatctt cgccacggcc
     1081 acggcctcct cctccctgag atcggggagt atggtgggga ggcgcctggc caccatggtc
     1141 ttgcccgagc ccggagggcc gaagaggagg aggtgatggc cgcccgcggc ggcgatctcg
     1201 agggcccgcc tggcgcggcc aaggcccctg atctccgaaa ggtcgccggc ttcggcgccc
     1261 tgggccggct gagcctccct cggacggcgt ggctccggat cccgcccctc ccggagcctt
     1321 acgagcgtct cagcggcctc ggagagggcg ccgatgccat ggatgacgcc cctgcccacg
     1381 gccatggcct cctcgacgtt cgcccgcggc acgataaaac cctcgattcc cgccttgagg
     1441 ccggccgcga ccgcgggaag gacgccgcgc acggggcgca cggagccctc gaggccgagc
     1501 tcgcccaggg ccaggaaggg ccgccccgga tcgggaagga gacctgccgc tccgaggacg
     1561 gccagggcga tgggcaggtc gtaggccgag ccctccttgg gcaggtctgc cggaacgagg
     1621 ctcacgagga cgcggtcctg ggggaattcg aagccggcgt tgcggacggc cgcccgcacg
     1681 cgctccctcg cctcgcgcac ggccccttcg gcgaggccga cgaggtccac ctgcggtatg
     1741 ccccggcgga tgtccacctc gacgaggacg aggacccctt cgaagccgga gggctcgtgg
     1801 gcggcgactg tcatgatggc ctccgccctg gctacgcgcg cgtggccgcg cggcgcttca
     1861 agagaggccc cttcgcgagc ggacgcttcc tccgcctacg ggggatcgac aaagcgccag
     1921 gcgcttcttc gccgcgagcg gacgctgccg cctacggcta gtcgcggaag cgctccgcgc
     1981 ttccgcgccg tcgcaggacg cgctctccgg cgatcgacga agcgccatgc gcttcgtcgc
     2041 cgtcggcgga cgctaccgcg tccgccgacg aaggatcatc acgaggaagg cggcggagag
     2101 gaagaggtag ggcaggttcg agaagacgat gcgcccgagg gggaggccga agggcgagag
     2161 acgggcctcg tccaggaggc cgaagcgcac gaggaggtcg aagagtatgt cggcgtagct
     2221 cgctatggcc ccgatgacga tgagcatcca ggccaggtcg cgggtcctcg accagaggac
     2281 gatggccagg aaggccgtcg tcgcggcgac ggcgaggcgg gagacgaggt acacggcgag
     2341 tcctgcgttc atatcgtccc catagtatcg gcgcagccgg actcgttctt gacaggaggc
     2401 gggagggggc ctaccttggg acgaggaggc gaagcgtgga cctttggtac agccgtcccg
     2461 agagcccggg ctccgatttc cgagtgaagg tgcggaggcc cctcttctcc gcggagtcgg
     2521 gagagcgccg cctcgacgtc ctggagaccg aggacttcgg ccgcctcctc ctcgtcgacg
     2581 gccggatcgt cctggccgag aacgacggag ccgcctaccg cgagatggcc gtccacgtgc
     2641 cgctcaacgc gcatcccggc gtccgctccg tcctcgtggc cggcggcggg gacggctcgg
     2701 tcgtggccga gctcgtgcgc taccccgagg tggagcggat cgtcgtcgcc gagcccgacg
     2761 acggagtcgt ggaggcggcc aggcgctggt tccccgggat cgcggcctcc ttgcgcgacc
     2821 cgcgcgtgcg cctcgtggcc gaggaagcgg ccggcttcgc ccgcgagacc aaggagcgct
     2881 tcgacctcgt catcgtggac aggccggacg acgagggcaa ggccgagctc ggccaggcct
     2941 tctactgcga ctgcttccgg atactctcgg gggacgggat cctcgtgaac cgcgcgggga
     3001 gcgccacctt cccgggccgg cgccgcgacc tcgtgcgctc ggcgggccgc ctcaagcgcc
     3061 tctttcccgt ctaccgcctc taccgcgtct cgccgcccgc cggcgagccc ggggagctcc
     3121 tcctcggctt cgcctccaag cgctacgagc cctccaaggg gccggacgag gaggcctggg
     3181 agaagcgggg catcgcgacg agctactaca acgcggccct tcactgcgcc gccttcgccc
     3241 tgccccagta cgcccaggaa ctcctggaag gcgtctagga cccccggacc gcggcgcccg
     3301 aggcggcgcc gcccggggca acggcgcccg ggcaatggcg ctctcaggcc gcgccaccct
     3361 aggccgcggc cgctcgagga tggcctgcct gactacctac ggggattcgc cttggcgtac
     3421 tcctggtaga tgaagcgctt gatcttccgc gtgctcgtct tctcgaactc ctcgtccttg
     3481 acgaggaagt cggcgagctt catgtagggc ggcagggtct tgttgacccg ctggacctcc
     3541 tcgcggagga gggactcgac gaaggcggcg tccttctcgc gccccggatg ggccagggcc
     3601 agcttctcga agtcggggac gaggacggcg atgacctcct cgccgggatg gccccggcgc
     3661 gaggggcggc cgacgaccag ggactccttg atccaggccg agccctcgaa gcggccctcg
     3721 atctcctcgg gatagacgtt cttcccgccc tccgtgacga tgaggctctt ccgccggccc
     3781 gtgatgaaga cgaagccgcg ggcgtcgacg cgcccgaggt cgcccgtctt gagccagccg
     3841 tcctccgtga gggcctcgcg gctcgcctcg gggttgtcga ggtagccctc catgagggag
     3901 gggctccgca cctggatctc gccgatcccg tcggggccgg gctcgccgat gcggagctcc
     3961 gtgtgcttga ccgggaagcc gaccgagcgg ttgtccttgc gcttgggcag gttgacggag
     4021 atgagggggc cgttttcgct catcccgtag ccctggacca ggttgatgcc gagggcgtcg
     4081 aagaagtcgg ccgtcgcggg ggctaggggt ccgcctcccg agaccgcgag gcggaccgtg
     4141 cccaggcccg ccttctccct gacgggcctg aagaggaggc ggccgagggg caggcc
//
LOCUS       DAOOSJ010000231         4186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13409_length_4186_cov_6.426289, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000231 DAOOSJ010000000
VERSION     DAOOSJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_4186_cov_6.426289"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="ffh"
                     /locus_tag="PLG14_10365"
     CDS             <1..1296
                     /gene="ffh"
                     /locus_tag="PLG14_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOX49577.1"
                     /translation="ISEKNVEEAVEQIKLALLEADVNLRVVRRFVNSTLEEAKGEKVL
                     RSVQPGQQFVKIVHDKMVALLGEGRQDLVLKGRDTTSVILLLGLQGSGKTTSASKLAF
                     RMKGEGRKVLLAACDLVRPAAVEQLSVLGAQIGVDVYKEEGAKDPVKVASAALAKARR
                     ELYDLLIVDTTGRLEVDEAMMAELVRVRDAVKPDESLLVADAMTGQAAADIAKAFDEK
                     VGVTGVVLSKFDSDARGGAALSLKSVTGKPVKFVGVSEKPEGLEPFYPERVAGRILGM
                     GDVVGLVEKAQAAFDQTEALELEKKMANESFTLEDYLDQIAKVKKMGSMKGMLDMIPG
                     LAGQIDEDRIDEAGIKYEEAILRSMTRKERANHLIIGPKRRTRIAKGSGTSVAEVNRL
                     LKKFEKTRLMMKKMVKNKGQMAQMAQMMGQGQGPVGRRG"
     gene            1391..1582
                     /gene="rpmB"
                     /locus_tag="PLG14_10370"
     CDS             1391..1582
                     /gene="rpmB"
                     /locus_tag="PLG14_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOX49578.1"
                     /translation="MSRKCDICGKGTIFGNTVSHAKNRSRRTWFPNLLEVRADIGGTV
                     KTVKICTRCLKAGKVTKVV"
     gene            1768..2664
                     /locus_tag="PLG14_10375"
     CDS             1768..2664
                     /locus_tag="PLG14_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOX49579.1"
                     /translation="MPELPDLVHIVRALGPALLGRRVEAVRVKNPLVLRVLLPGDFGA
                     LLEGRRFEELERHGPFLRFGLGAAGLPAGAGAAGEGARIDVVAHLMLAGRLRLAPRGE
                     KPLAYTAFALDLDSGETLSYGDEKSMGKIYLCARGCFEAIPGYLSQGVDVTGPGFTYE
                     AFSALIAKKRCQARVFVMDQSSLSAIGNAYADEILFEAGIHPKAPCSSLPEERRRALY
                     EAVKSVLAWGTEEVEKAGRPLEDKVRGHLRVRNRAGEACPRCGATIRKAGVLGFDAFF
                     CPICQPDKAGRGLDWSRLEGPR"
     gene            2766..3659
                     /locus_tag="PLG14_10380"
     CDS             2766..3659
                     /locus_tag="PLG14_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49580.1"
                     /translation="MIHLESLVGLAGAEGAARALLAACEAADGSASLSFEKSLDAHPD
                     LPSWFLAREGGAGSPLVGLASVFCPRREEAEISAFVAPGARRRGVFSALLARLEEELA
                     GSGYESELLVVERGSPGGAETAAAFGALLDFSEYELAHPGPEALTAALAGSGKALPQA
                     LALRRATEADFESLLGVYTEAFGESRENEESMLRSSLSSATRTQYAAWAGGRVVGAAA
                     LTDEGGSFSVNGVAVLGSERGKGYGKAIVARCLELVFARGSRAVIEVDSTNDIAYRTY
                     LGLGFEVKRAIDYWRRPFPSA"
     gene            3755..4126
                     /gene="crcB"
                     /locus_tag="PLG14_10385"
     CDS             3755..4126
                     /gene="crcB"
                     /locus_tag="PLG14_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOX49581.1"
                     /translation="MIDLALVFAGGGLGAASRWLASRLALRLIGSEFPWGTLAVNLAG
                     CFLIGLSVGALERGLLPPRLRPALVSGFLGGFTTFSTYAFGTFELMRHGAWGKALLQA
                     AASNLLGVLLAALGWALAPRR"
BASE COUNT          604 a         1535 c         1416 g          631 t
ORIGIN      
        1 atcagcgaga agaacgtcga ggaggccgtc gagcagatca agctcgccct cctcgaggcg
       61 gacgtcaacc tccgcgtggt ccgccgcttc gtcaactcca ccctggagga ggcgaagggc
      121 gagaaggtcc tccgctccgt ccagccgggc cagcagttcg tcaagatcgt ccacgacaag
      181 atggtcgccc tcctgggcga gggcaggcag gacctcgtcc tcaagggcag ggacacgacc
      241 agcgtcatcc tcctcctcgg cctccagggc tcgggcaaga cgacgagcgc ctcgaagctc
      301 gccttccgga tgaagggcga ggggaggaag gtcctcctcg ccgcctgcga cctcgtgcgc
      361 ccggccgccg tggagcagct ctccgtcctc ggcgcccaga tcggcgtcga cgtgtacaag
      421 gaagagggcg cgaaggaccc ggtgaaggtc gcgtccgcgg ccctcgccaa ggccaggcgc
      481 gagctctacg acctcctcat cgtcgacacg acgggccgcc tggaggtcga cgaggccatg
      541 atggccgagc tcgtccgcgt ccgcgacgcc gtcaagcccg acgagtccct tctcgtcgcc
      601 gacgcgatga cgggccaggc cgccgccgac atcgcgaagg ccttcgacga gaaggtcggc
      661 gtcacgggcg tcgtcctctc caagttcgac tccgacgcca ggggcggcgc cgccctctcc
      721 ctcaagtccg tcaccggcaa gcccgtcaag ttcgtcggcg tctccgagaa gcccgagggc
      781 ctcgagccct tctacccgga gcgcgtggcc ggccgcatcc tgggcatggg cgacgtcgtc
      841 ggcctcgtgg agaaggccca ggccgccttc gaccagaccg aggccctgga gctcgagaag
      901 aagatggcga acgagtcctt caccctggag gactacctgg accagatcgc caaggtgaag
      961 aagatgggct ccatgaaggg catgctcgac atgatccccg ggctcgcggg ccagatcgac
     1021 gaagacagga tcgacgaggc cggcatcaag tacgaggagg ccatcctccg ctcgatgacg
     1081 aggaaggagc gcgcgaacca cctcatcatc ggccccaagc gccggacgcg catcgccaag
     1141 ggctcgggca cctcggtcgc ggaggtcaac cgcctcctca agaagttcga gaagacccgc
     1201 ctcatgatga agaagatggt gaagaacaag ggccagatgg cccagatggc ccagatgatg
     1261 ggccagggcc aggggcccgt cggccggagg ggctaggctt cctgccgccc cgaaggggcg
     1321 gtttccggga ctttacacgg gagggcgact tctgctagta tcgccctacc atcccgatcg
     1381 gagttatacc atgtcgagaa aatgcgatat ttgcggtaag ggcactatct ttggaaatac
     1441 cgtctcccac gccaagaacc ggagccgccg gacctggttc cccaacctcc tcgaggtcag
     1501 ggccgacatc ggcggaaccg tcaagacggt caagatctgc acccgctgcc tcaaggccgg
     1561 caaggtcacc aaggtagtct gattcccggg gcggggcggc gagtcccgtt cgcgatgaaa
     1621 agccgccctt ttcgggcggc ttttccgttt ttccgcgcct ttcccccgtt ttgggccttc
     1681 cagccgcccc gcggcaagca cgaagacagg acgacgcccg cgacttcccg gcttgccgag
     1741 gcggctccgg gagcctaagc tcggggcatg cccgagcttc ccgacctggt ccacatcgtc
     1801 cgcgccctcg gtcccgccct ccttggccgg cgggttgaag ccgtccgcgt gaagaacccc
     1861 ctcgtcctcc gcgtcctcct ccccggcgac ttcggcgccc tcctcgaggg aaggcgcttc
     1921 gaggagctcg agcggcacgg ccccttcctc cgcttcggcc tcggggcggc cggcctcccg
     1981 gccggagccg gcgcggcggg cgagggcgcc aggatcgacg tggtggccca cctcatgctc
     2041 gccggccggc tccgcctggc cccccgcggg gagaagcccc tcgcctacac cgccttcgcc
     2101 ctggacctgg actcggggga gaccctgtcc tacggcgacg agaagagcat gggcaagatc
     2161 tacctctgcg cgcggggctg cttcgaggcg atccccggct atctctccca gggcgtcgac
     2221 gtgacggggc cgggcttcac ctacgaggcc ttctccgccc tcatcgcgaa gaagcgctgc
     2281 caggcgcggg tcttcgtcat ggaccagtcc tctctctccg cgatcgggaa cgcctacgcc
     2341 gacgagatcc tcttcgaggc gggcatccac cccaaggcgc cctgctcctc cctgcccgag
     2401 gagaggcgcc gcgccctcta cgaggcggtg aagtccgtcc tggcctgggg gacggaggag
     2461 gtcgagaagg cgggacggcc cctcgaggac aaggtccgcg gccacctgcg cgtgcggaac
     2521 cgggcgggcg aggcctgccc ccgctgcggc gcgacgatcc ggaaggccgg cgtcctgggc
     2581 ttcgacgcct tcttctgccc gatctgccag cccgacaagg cggggcgggg cctggactgg
     2641 tcgaggctcg aggggccaag gtaggggccg cggccgcggc gaccttctgc gcggcggagg
     2701 attcttcgtt tctccgcaag aagggcactt cccgcccgcg gcggcctggg ataggctcgg
     2761 agccgatgat acacctagaa agcctggtcg gactcgccgg ggccgagggc gccgcgcggg
     2821 cccttctcgc cgcctgcgag gccgctgacg gctcggcctc cctctccttc gaaaagagcc
     2881 tcgacgccca cccggacctg ccctcctggt tcctggcccg ggagggggga gcaggctctc
     2941 ccctcgtcgg cctcgcctcg gtcttctgcc ccaggcggga ggaggcggag atcagcgcct
     3001 tcgtggcgcc cggcgcgcgg cggaggggcg tcttctcggc ccttctcgcc aggctcgagg
     3061 aggagcttgc gggctcaggc tacgagtcgg agctcctggt cgtcgagcgc ggctcgcccg
     3121 gtggagcgga gacggccgcc gccttcggcg ccctcctcga cttcagcgag tacgagctcg
     3181 cccatccggg gcccgaggcc ctcacggcgg ccctcgccgg ctccggcaag gcacttccgc
     3241 aggccctcgc cctcaggcgg gcgacggagg cggatttcga gagcctgctc ggcgtctaca
     3301 ccgaggcctt cggcgagtcg cgggagaacg aggagtcgat gctccgctcc tcgctctcct
     3361 ccgcgacgcg gacgcagtac gccgcgtggg ccggaggccg cgtggtcggg gccgccgcgc
     3421 tcaccgacga gggcggaagc ttctccgtca acggcgtcgc cgtcctcggc tccgagcggg
     3481 gcaagggcta cgggaaggcc atcgtcgccc gctgcctgga gctcgtcttt gcccgcggct
     3541 cgcgggccgt catcgaggtc gactccacga acgacatcgc gtaccggacc tacctgggcc
     3601 tcggcttcga ggtgaagcgg gccatcgact actggcggcg gcccttcccc tcggcctagg
     3661 cggccagccg gcctccgggc cgcgccttgc cgcgctgagt ctttttcggc gcggcgcctt
     3721 cccccgcgcg acgcggccgg gctagaatcg gcccatgatc gacttggccc tcgtcttcgc
     3781 ggggggcggc ctgggagccg cctcgcgctg gctcgcctcc cgcctcgccc tgcgtctcat
     3841 cggctcggaa ttcccctggg gcaccctcgc ggtcaacttg gcgggctgtt tcctcatcgg
     3901 gctgtcagta ggcgccctcg agcgcggcct ccttccgccg cgcctccggc cggccctcgt
     3961 ctccggcttc ctgggcggct tcaccacctt ctcgacctac gccttcggca ccttcgagct
     4021 catgcgccac ggcgcctggg gcaaggccct cctccaggcc gcggcctcga acctcctcgg
     4081 cgtcctcctg gccgcactgg gctgggccct cgccccgcgc cgctagcgcg gtcatctccc
     4141 gggcgccgcg acggggcgta gggccgctgc tataggcgcc gcgccg
//
LOCUS       DAOOSJ010000232         4181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13429_length_4181_cov_5.640330, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000232 DAOOSJ010000000
VERSION     DAOOSJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_4181_cov_5.640330"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1032
                     /locus_tag="PLG14_10390"
     CDS             52..1032
                     /locus_tag="PLG14_10390"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOX49582.1"
                     /translation="MTIDEAAGFLRAHDDYLIAPHDGPDADGLGAAYALAIALELAGK
                     RAYPLLSDEAPAKYRFIDRRGLFRNLAAGEEPPFDPSAATLVIVDTHDLGYLGQRVER
                     FVDETRDLLVIDHHEVKTGQKPYHCIDPAASSTCELVFLIAKALGVELPEDAAEAVFS
                     GIVYDTGSFAYPKTGERTFACALELVKLGVKPYIVHNRMYESSSIGALLLQKTVLSSL
                     ELRSENRIAVQTLRRADLAASGATYEDAEDLINLPLQGRTVEVSLLFKENLEGRLRCS
                     LRSKGAVNVAHVAQGFGGGGHKTAAGFTCDRPLERMKEAVLESIVRALEG"
     gene            1082..2593
                     /locus_tag="PLG14_10395"
     CDS             1082..2593
                     /locus_tag="PLG14_10395"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HOX49583.1"
                     /translation="MKKFVQALFLVFCAIALGVAVYFYISTRSGRAEASRPVATRQLE
                     LRSGADAAGEREAAAEREAATNRISALPNEVVLDVVTVNLDQDEGDEQILIVRKTDKA
                     ESLLSVVVADYLPQRRAWIRAWEGETLATKLTTFSIQVRDVLGDHNLAIVCTGINDSN
                     EQTVTVFRRIPGSDPSVLSYALVCAVAADAVVVEELERTEGYQLGQVNGVSWPVVAYR
                     SDKESQNFLDQVKTTYAWDFRKGSYVESGSEKITGAEVERGMAAKVLTGVEKDFEAYL
                     TGVWEATAPAPSGPGARLLVFDRPGDTITFFSSDAQEVFHWNESHSTRYGLYVGCQNE
                     SVSNLRRLMDIELTGADAISVRIFEDVQMKVDAEKRWDGSYRKLPPGSGGRPEAEARR
                     EAPLKLEGIYRSADGAELVFEGSRFTLSKGQTVERGGFVAYSLGRDSVLELTSLKESG
                     LVSSRRIYRVAFAETRNGRSISRKLRLSPARAAISGLELLEEPELSFEQKIGD"
     gene            complement(2621..4045)
                     /locus_tag="PLG14_10400"
     CDS             complement(2621..4045)
                     /locus_tag="PLG14_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX49584.1"
                     /translation="MRRLGRLVRPIGRLGRLGRGRGRGARAWTLPVRPVGFGAALFLG
                     ASLFLSASPIAQAAPAAPQATAPSQAAARLEALRAELRAAKGQAGFASLLEARAGAAQ
                     PADAAAILSEFAPLVADPAASKALMLRGASLCLLLGRLPEAAALYEGAAFRPGLPRDE
                     ALLLKAARLRLAAGEAERAAEIAGIVQGGTKVEELLREARLVSAWAALLSGAQDRAAA
                     LAAEVAKGAPAGSAARREARFLAWAAAAESGRPEAARLLASEYPGSPEARIAVGQAPG
                     AAGASPPKGQAASVELSPLPHWYLSGVLAGPGLDRPAPGSLAEPQAGAAGSQPAAAVQ
                     GSQPPGAAKPAASAPDAAATPGTAAAKPAAPAAALSPAAATPAPAAPAAGSTPAPAAA
                     AAPSAPTAYQVGVFSKPENAARLLEELKAKGFKARTEKRISGGRELVAVVVEGYSGAK
                     GGPADGEGLLLRLKDSGYEAYPLF"
BASE COUNT          631 a         1526 c         1481 g          543 t
ORIGIN      
        1 tcatcctggc gctaaaattg ctaaagaagc gccgaagcgg cgaggagcgg aatgaccatt
       61 gacgaagcgg ccggattcct ccgtgcccac gacgattacc tcatcgctcc ccacgacggg
      121 cccgacgccg acgggctcgg ggctgcctac gccctggcca tagccctgga gctggccggc
      181 aagcgcgcct accccctcct ctccgacgag gcccccgcca agtaccgctt catcgaccgc
      241 cgcggcctct tcaggaacct ggcggcgggc gaggagccgc ccttcgaccc ctccgccgcg
      301 accctggtca tcgtcgacac ccacgacctg ggctacctgg gccagcgcgt cgagcgcttc
      361 gtcgacgaga cccgcgacct cctcgtgatc gaccaccacg aggtgaagac cgggcagaag
      421 ccctaccact gcatcgatcc ggccgcctcc agcacctgcg agctcgtctt cctcatcgcg
      481 aaggccctgg gcgtcgagct gccggaggac gcggccgagg cggtcttctc cggcatcgtc
      541 tacgacacgg gctccttcgc ctacccgaag accggcgaga ggaccttcgc ctgcgccctg
      601 gagctcgtca agctcggggt gaagccgtac atcgtgcaca acaggatgta cgaatcctcc
      661 tccatcggcg ccctcctcct ccagaagacc gtcctctcct ccctggagct ccgctccgag
      721 aaccggatcg ccgtccagac cctgaggcgg gccgacctcg cggcctccgg cgccacctac
      781 gaggacgccg aggacctgat caacctcccc ctccagggcc gcaccgtcga ggtctccctc
      841 ctcttcaagg agaacctgga ggggaggctc cgctgctccc tgcgctcgaa gggcgccgtc
      901 aacgtcgccc acgtcgccca gggcttcggg ggaggcgggc acaagaccgc cgcgggcttc
      961 acctgcgacc gcccccttga acgcatgaaa gaggccgtgc tagaaagcat cgtccgggcc
     1021 cttgagggct aaagccaggc cttccggacc caagccccgc ccgcccccct tccgaggtcc
     1081 catgaagaag ttcgtgcaag cgctgttcct cgtcttctgc gccatcgccc tgggcgtggc
     1141 cgtctacttc tacatcagca cgcgctccgg cagggccgag gcgagccgcc ccgtcgccac
     1201 ccgccagctc gagctccgct cgggcgccga cgccgcgggc gagcgggagg cggcggccga
     1261 gcgagaggcc gccaccaacc ggatctccgc cctgcccaac gaggtcgtcc tggacgtggt
     1321 caccgtcaac ctggaccagg acgaggggga cgagcagatc ctcatcgtcc gcaagacgga
     1381 caaggcggag agcctcctct ccgtcgtcgt ggccgactac ctgccccagc gccgcgcctg
     1441 gatcagggcc tgggagggcg agaccctcgc caccaagctc acgaccttct cgatccaggt
     1501 cagggacgtg ctcggcgacc acaacctggc catcgtctgc accggcatca acgactccaa
     1561 cgagcagacc gtgacggtct tcaggcgcat accgggctcc gacccctcgg tgctctccta
     1621 cgccctcgtc tgcgcggtgg ccgccgacgc ggtcgtcgtg gaggagctcg agcgcacgga
     1681 gggctaccag ctcggccagg tgaacggcgt gagctggccc gtggtggcct accggagcga
     1741 caaggagtcc cagaacttcc tggaccaggt gaagaccacc tacgcctggg atttcaggaa
     1801 gggctcctac gtcgagtcgg gcagcgagaa gatcacgggc gccgaggtcg agcgcggcat
     1861 ggccgccaag gtcctgaccg gcgtcgagaa ggacttcgag gcctacctca cgggcgtctg
     1921 ggaggcgacc gcgccggccc cctccggccc gggcgcccgc ctcctcgtct tcgaccggcc
     1981 gggggacacg atcaccttct tctcctccga cgcccaggag gtcttccact ggaacgagtc
     2041 gcattcgacg cgctacggcc tctacgtggg ctgccagaac gagtcggtct ccaacttgag
     2101 gcgcctcatg gacatcgagc tcacgggcgc ggacgcgatc tccgtgcgca tcttcgagga
     2161 cgtgcagatg aaggtggacg ccgagaagcg ctgggacggc agctaccgga agctgccccc
     2221 gggctcgggc ggaaggccgg aggccgaggc caggcgggag gcgcccctca agctcgaggg
     2281 gatctaccgc tcggccgacg gcgccgagct cgtcttcgag ggctcgcgct tcaccctctc
     2341 caaggggcag acggtcgagc gcggcggctt cgtcgcctat tccctgggcc gcgactccgt
     2401 cctcgagctc acgagcctca aggagtcggg cctggtctcc tccaggcgga tctaccgcgt
     2461 ggccttcgcg gagacgagga acggccgctc catctcgagg aagctcaggc tctcgcccgc
     2521 gcgcgcggcg atctcgggcc tcgagctcct ggaggagccc gagctctcct tcgagcagaa
     2581 gataggcgac tgaaagtccc cgggccggcc gaggcccggg ctagaagagc gggtaggctt
     2641 cgtagcccga gtccttgagc cggaggagga ggccctcgcc gtccgcgggg cctcccttcg
     2701 cgcccgaata gccctcgacc accacggcca cgagttccct acccccggag atcctcttct
     2761 ccgtcctcgc cttgaagccc ttggccttga gctcctcgag gagccgcgcc gcgttctcgg
     2821 gcttggagaa gaccccgacc tggtaggccg tgggggctga cggggcggcc gccgcggcgg
     2881 gcgcgggcgt cgagccagcg gccggggccg cgggcgcggg cgtcgcagcg gccggagaca
     2941 gggccgcagc cggcgcggct ggcttggccg cggcggtccc cggggtcgcg gccgcgtcgg
     3001 gagcagaggc agcaggcttg gccgcgccgg gaggctggct cccctggaca gctgcggccg
     3061 gctgggatcc cgcggccccc gcctggggtt ccgcgaggga acccggggcg ggccgatcca
     3121 ggcccggacc cgcgaggacg ccgctcaagt accaatgggg aaggggcgat agttccaccg
     3181 aggcggcctg ccccttcggg ggagaggccc cggccgcccc gggagcctgg ccgacggcga
     3241 tgcgggcctc ggggctaccg ggatactcgg aggcaaggag acgggccgcc tcgggccttc
     3301 ccgattcggc ggcggcggcc caggcgagga agcgggcctc ccgcctcgcc gcgctcccgg
     3361 ccggcgctcc cttcgcgacc tcggccgcca gcgcggcggc cctgtcctgg gcccccgaaa
     3421 ggagggcggc ccaggccgag acgaggcgtg cctcgcggag aagctcctcg accttggtcc
     3481 ctccctggac aatgcccgct atctcggcgg cacgctcggc ctcgccggcc gcgagcctga
     3541 ggcgcgccgc cttgaggagg agggcctcgt cccgcgggag gccgggcctg aaggcggcgc
     3601 cttcatagag ggccgcggcc tcgggcagac gccccaggag gaggcagagg gaagcgccgc
     3661 ggagcatgag ggccttcgag gcggccgggt cggccacgag gggggcgaat tcggagagga
     3721 tggccgcggc gtcggcgggc tgggccgcgc cggccctggc ctcgaggagg gaggcgaagc
     3781 cggcctggcc cttggccgct cggagctctg cccgcagggc ctcgagccgg gcggcggcct
     3841 gggagggcgc cgtagcctgg ggcgctgccg gcgccgcctg ggcgatcggg gaggcgctca
     3901 gaaagaggga ggcgcctagg aagagggcgg cgccgaagcc gacgggtcgg acagggagcg
     3961 tccaggcgcg agcgcctcgg cctcggccgc ggccaaggcg gccaaggcgg ccaattggcc
     4021 ggacgaggcg accgaggcgc ctcaaggctc gacctcctcg gccttgaagg tgttgcccga
     4081 aagggagagg aggcggaccc gcgcgaagcc gcccacgcgg gaggccgggg cggggaagac
     4141 caccatctcg tcggcctggg tccgcgcgag gacctcgctc t
//
LOCUS       DAOOSJ010000233         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13551_length_4157_cov_5.726962, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000233 DAOOSJ010000000
VERSION     DAOOSJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13551_length_4157_cov_5.726962"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLG14_10405"
     CDS             complement(<1..1087)
                     /locus_tag="PLG14_10405"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOX49585.1"
                     /translation="MSAQSIAHIPTIRLSGGETIPLEFHKVRVVQRLTLAPAAGRVAA
                     IRKAGFNTFLLPTSAVFLDMLTDSGTNAMSDAQVAAMSAPDDAYAGSASFDRLSAAAR
                     ELFGKELVLPAHQGRAAEHVICQALVKKGSVVPMNYHFTTTRAHIELAGGSIVELLRP
                     GAVASRSTEPFKGDIDVAALEKVLDANPGRVPFVRMEASTNLIGGQPFSLANLRAASA
                     AARSRGIPLVLDASLLGENVWFMKTREAACAGMAIPEIYRAVSACADIVYFSARKLSS
                     SRGGAILTSDPALYGAMRDLIPLFEGFLTYGGMSTREMEAIAVGLRESVDEAVIGQSP
                     TFIEHLASRLDAAGVPVVLPAGALGCHV"
     gene            complement(1255..1386)
                     /locus_tag="PLG14_10410"
     CDS             complement(1255..1386)
                     /locus_tag="PLG14_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49586.1"
                     /translation="MRIPDPTSAPAGAPIRSLSLSFATSSPLLSSSYTPYSRYLYSY"
     gene            complement(1544..2188)
                     /locus_tag="PLG14_10415"
     CDS             complement(1544..2188)
                     /locus_tag="PLG14_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX49587.1"
                     /translation="MEASAQRVFEAGETIFREGEKGDLMYVLLEGAVELKKKVERGEA
                     VLKVVDTPNDFFGEMALLDDRPRSATAVAERRSRVLVVDRPSFESMILQNGRFALKII
                     KVLAERIRRSNDQVSELIETMPKERISRGMADFAMRAGERIHDGSYKVSVAGMSAWIN
                     GHLGVPLDELEAAIFRLVKAESISYSKAKDHLLLPEAFVRTNDRRAGEAPDRDD"
     assembly_gap    2274..2373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2374..2636)
                     /locus_tag="PLG14_10420"
     CDS             complement(<2374..2636)
                     /locus_tag="PLG14_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49588.1"
                     /translation="MDLAAFAALRDRDLRAEGLVVAEGRLLAERLLAASRLEVLGVLC
                     VPALAGRFEELAAGRCPVLVRPEAALAETAGYPFHRGVIAAARR"
     gene            complement(2661..3734)
                     /locus_tag="PLG14_10425"
     CDS             complement(2661..3734)
                     /locus_tag="PLG14_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOX49589.1"
                     /translation="MTSFLETELAGRPLGLWLEASLYILGGFLAGLVVSRVSSGILKK
                     LSAKTRNRLDDIILAVTEKPLVFVLVLVGLRLGLEHLALGAESAALVDKAYYILVAVA
                     AGWVLGRIIDAITQEFLVPLVEKTEGDLDDQLLPIVRKIGKVVVWALAILVGLKNAGY
                     DVGALIAGLGIGGVAVALAAKDTLANFFGSLAIFIDRPFKMGDRIKVNGVDGTVVDIG
                     IRTSKLKTLDNRIVTMPNLSFSSSPIENVSSEPSTKIVEYLDLTYATGAAGVERALAI
                     LRALPSSIEGIEENVVCGLSAFAESSLRLTFVFFIKKGADYLGTIDAVNRAVLSSFGE
                     AGIDFAFPTRVVVGQGAASAASR"
     gene            3876..>4157
                     /locus_tag="PLG14_10430"
     CDS             3876..>4157
                     /locus_tag="PLG14_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOX49590.1"
                     /translation="MTRSELIDYCLAKPKAKGDFPFDDRTLTVRVGKKIFLLVDLEAE
                     EESANLKCEPELSADLRAAYPAIKPGYHMNKEHWNTVLLDGSLPDELVRS"
BASE COUNT          636 a         1282 c         1561 g          578 t
ORIGIN      
        1 cgacgtggca gcccagggcg ccggccggga gcacgacggg gacgcccgcc gcgtcgaggc
       61 gcgaggcgag gtgctcgatg aaggtcggcg actggccgat cacggcctcg tcgaccgatt
      121 cgcgcaggcc gacggcgatg gcctccatct cgcgggtcga catgccgccg taggtcagga
      181 agccctcgaa gagggggatg agatcgcgca tcgcgccgta gagggccggg tccgaggtga
      241 gtatggcgcc gccccgcgag gaggagagct tccgcgcgga gaagtagact atgtcggcgc
      301 aggccgagac cgcgcggtag atctcgggga tcgccatccc ggcgcaggcg gcctccctcg
      361 tcttcatgaa ccatacgttc tcgcccagga ggctcgcgtc gaggacgagg gggatgcccc
      421 tcgagcgggc ggccgccgag gccgcgcgga ggttggccag ggagaagggc tggccgccga
      481 tgaggttggt cgaggcctcc atgcgcacga agggcacgcg gccgggattc gcgtcgagga
      541 ctttctccag ggcggccacg tcgatatcgc ccttgaaggg ctcggtcgag cgcgaggcga
      601 cggcgccggg gcggaggagc tcgacgatcg agccgccggc gagctcgatg tgggcgcgcg
      661 tggtggtgaa gtggtagttc atgggtacca cggagccttt cttcaccagg gcctggcaga
      721 tgacgtgctc cgcggcgcgg ccttggtgcg cgggcagaac gagctccttg ccgaagagct
      781 cgcgggcggc ggccgatagc cgatcgaagc tcgccgagcc ggcgtaggcg tcgtccggcg
      841 ccgacatggc ggcgacctgg gcgtcgctca tcgcgttggt gcccgagtcg gtgagcatgt
      901 ccaggaagac tgcgctcgtg gggaggagga aggtgttgaa gcccgccttc cggatcgcgg
      961 ccacgcggcc ggcggccgga gcgagcgtca ggcgctggac gacgcggacc ttgtggaact
     1021 cgagggggat ggtctcgccg cccgagaggc ggatcgtggg gatatgcgcg atggattggg
     1081 cgctcatggc gtcctcctgg tggtgcgtgg ttttccccgc cgtcgcgggc ggggggctcg
     1141 cgccgcgcgc gcgaggcgcg atggcgcgat aggcgatatt gcgagcctag gggctcgggc
     1201 tcggggaggg agcgggttta tcgggggatg gcctgcggcg cccgacgcgc cgcgtcaata
     1261 ggagtagagg taccgggagt agggggtgta ggaggaggag aggagggggg aggaggtggc
     1321 gaaggaaagg gagagggatc ggatcggcgc ccccgcgggc gcgctggtcg gatcggggat
     1381 tctcacggcg acgctccttt cgacatgcgt actatgcgga gaatcatgcc gcgccgggcc
     1441 ttcgctgtca agcgctttcg gcggccgaat gcgcggaccg ccgttgcggc ggaccggagc
     1501 cgcggcggac cggccgcgcc gccgggcgcc gccggcgccg cggctagtcg tcccggtcgg
     1561 gagcctcgcc ggcgcgccgg tcgttcgtcc tcacgaaggc ctcgggaagg aggaggtggt
     1621 ccttggcctt cgagtaggag atcgactcgg ccttcaccag gcggaatatg gcggcctcga
     1681 gctcgtccag ggggacgccg aggtggccgt tgatccaggc tgacatgccc gcgacggaga
     1741 ccttgtagct gccgtcgtgg atgcgctccc cggcgcgcat ggcgaagtcg gccatgccgc
     1801 gcgatatgcg ctccttgggc atcgtctcta tgagctcgga gacctggtcg ttggagcggc
     1861 ggatgcgctc ggcgagcacc ttgatgatct tgagggcgaa gcggccattc tgcaggatca
     1921 tcgactcgaa gctggggcgg tcgaccacga ggacgcggga gcgccgctcc gctaccgccg
     1981 tcgccgagcg gggcctgtcg tccaggaggg ccatctcgcc gaagaagtcg ttcggcgtgt
     2041 cgacgacctt gagcacggcc tcgccgcgct ccaccttctt cttgagctcg accgcgccct
     2101 cgagcaggac gtacatgaga tcgcccttct ctccctcgcg gaagatcgtc tctcccgcct
     2161 cgaacacgcg ctgcgcggat gcttccatgg gatgaattct accctttccg cggcgtccgg
     2221 gcaagggcgg cggcgggccg gcggcgaggg gaacgcggag cgcggcgcgg acgnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgccggg ccgccgcgat gacgccgcgg
     2401 tggaaggggt agccggccgt ctccgcgagg gcggcctcgg gccggacgag gacggggcag
     2461 cgtccggcgg ccagctcctc gaagcggccg gccagggccg ggacgcagag gacgcccagg
     2521 acctcgaggc gcgaagccgc gaggaggcgc tccgcgagga ggcgtccctc ggcgacgacc
     2581 aggccctcgg cgcggaggtc gcggtcgcgc agggccgcga aggccgcgag gtccaagggt
     2641 ccggctccgc ccgccatcgg ctagcgcgag gccgcggacg cggcgccctg gccgacgacg
     2701 acgcgggtcg ggaaggcgaa gtcgatcccc gcctcgccga aggaggagag gacggcgcgg
     2761 ttgaccgcgt cgatcgtgcc caggtagtcc gctcccttct tgatgaagaa gacgaaggtc
     2821 aagcggagcg aggactcggc gaaggccgag aggccgcaga ccacgttctc ctcgatcccc
     2881 tcgatcgagg agggcaatgc gcggaggatg gccagggccc gctcgacgcc ggccgctccg
     2941 gtcgcgtagg tgaggtccag gtactccacg atcttggtcg agggctcgga gctcacgttc
     3001 tcgatggggc tcgaggagaa gctcaggttg ggcatggtca cgatgcggtt gtcgagggtc
     3061 ttgagcttgg aggtgcggat gccgatgtcg accaccgtgc cgtcgacgcc gttcaccttg
     3121 atgcggtcgc ccatcttgaa gggcctgtcg atgaagatcg cgaggctccc gaagaagttg
     3181 gcgagcgtgt ccttggcggc cagggccacc gccacgccgc cgatcccgag gcccgcgatg
     3241 agggcgccga cgtcgtagcc ggcgttcttg aggccgacga ggatggccag ggcccagacc
     3301 acaaccttgc cgatcttgcg gacgatgggc aggagctggt cgtccaggtc gccctccgtc
     3361 ttctcgacca gggggacgag gaattcctgg gttatggcgt cgatgatccg ccccaggacc
     3421 cagcctgccg cgaccgccac gaggatgtag taggccttgt cgaccagggc ggcggattcc
     3481 gcgcccaggg ccaggtgctc caggccgagc ctgaggccga cgagcacgag gacgaagacg
     3541 aggggcttct ccgtgacggc gaggatgatg tcgtccaggc ggttcctcgt cttcgccgac
     3601 agcttcttga ggatgcccga ggacacgcgg gagacgacga ggccggccag gaagcccccc
     3661 aggatgtaga gcgacgcctc gagccagagc cccaggggcc ggcccgccaa ctcggtttca
     3721 aggaaggatg tcatgcgcac ctcgcggtct atgggatacg cgcaggatgc aggaaaaacc
     3781 ggccttccgc aagggcgtgc ggggtgccgc gctcccgccg ccgcccccct ggcgcggcgg
     3841 cccgaggaga agtaggctgg atggcaggag cagccatgac ccggagcgag ctgatcgact
     3901 attgcctcgc caagcccaag gcgaagggcg acttcccctt cgacgaccgc accctgacgg
     3961 tgcgcgtcgg gaagaagatc ttcctcctcg tggacctgga ggcggaggag gagtcagcca
     4021 acctcaagtg cgagcccgag ctctcggccg acctccgggc cgcctatccc gccatcaagc
     4081 ccggctacca catgaacaag gagcactgga acacggtcct gctcgacggg agcctccccg
     4141 acgagctcgt gcgctcc
//
LOCUS       DAOOSJ010000234         4148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13602_length_4148_cov_7.183484, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000234 DAOOSJ010000000
VERSION     DAOOSJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_4148_cov_7.183484"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLG14_10435"
     CDS             complement(<1..683)
                     /locus_tag="PLG14_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOX49591.1"
                     /translation="MKVSQEVQAIFNAAYNEAKLRNHEYLTPEHILYASLSFEEVRSI
                     LAACEADVDQLKRGMEAYFEQKIPQVKNAEPVQTAGFQSVIERAVLQSQAAGKEEVEV
                     ADILVSLFDEERNYSAYYLRKAGVKRLQLLEVISHGSEEGGDAYEEDEEEDEDEEEGH
                     EEREERRPQKGGRVGPLERFTSDLTKLAREGKLEPVIGRTAEIERTVQVLCRRLKNNP
                     IHVGDAGVG"
     gene            complement(687..998)
                     /gene="clpS"
                     /locus_tag="PLG14_10440"
     CDS             complement(687..998)
                     /gene="clpS"
                     /locus_tag="PLG14_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOX49592.1"
                     /translation="MANEPQNEGATLAREEEELKEPDEYRILLLNDDYTTMEFVVTVL
                     MTVFRKSLPEATRIMLDVHRKGKGVVGTYPYDIAATKINQVHELARQNGFPLKCTMEK
                     A"
     gene            1194..1784
                     /locus_tag="PLG14_10445"
     CDS             1194..1784
                     /locus_tag="PLG14_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX49593.1"
                     /translation="MEEEVIVDARALREDALSGGERAARAFGRLWSVYYRRLSAYAAS
                     FRGLPRGEREDAVAEALVSAFSRLESYDPGRPLSSWVYAIAHNRFADEARRARRLSPV
                     EVEAAAGPREGEGPPDGAHAEAIVSRLSDAELEAACAAAIAELPELDRRVAALSFLEG
                     LDSAEVGRVLGMAPGTVRWRIHRIRARVAGRVGGRP"
     gene            1781..2155
                     /locus_tag="PLG14_10450"
     CDS             1781..2155
                     /locus_tag="PLG14_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49594.1"
                     /translation="MTRLDRALKEALRREAEAADYRPPSGAELARALGPGRAAAFRAV
                     PSASARKAASLAAGFLALYASGFAAGLALSIWLKAPDAAALIGRSLPAKPGEGLYRFI
                     VELRPGWPGGAGRRGASAPPLE"
     gene            2166..2636
                     /locus_tag="PLG14_10455"
     CDS             2166..2636
                     /locus_tag="PLG14_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49595.1"
                     /translation="MLKRLLLPLSVAFLAVVGLVLLWGGGLWLFLDGYALVLVPGLGT
                     LYAFAMFGGRAGAGAFAAALDEGASPSDLRSAQAFFDALGRAYLAFAFLGAIVSFIDM
                     LKNLADRTQLGPRLAVACVSLEYAAILLVFLVLPFRTAAARRLAAEEEGGRPRA"
     gene            3152..>4148
                     /gene="thrS"
                     /locus_tag="PLG14_10460"
     CDS             3152..>4148
                     /gene="thrS"
                     /locus_tag="PLG14_10460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOX49596.1"
                     /translation="MSSNVETIRHSASHVLAEAVLRLYPGTKFGIGPAIEDGFYYDFQ
                     LPAPIQPEDLPAIEKEMRRIIASGAEFVRSEVSKEEARRRFADQPFKLELIDGLEEGS
                     ISLYEQDGFVDLCRGPHVATTREIRPDAFKLRAVAGAYWRGDEKRPMLTRIYAYAFAS
                     KADLEAHLKMLEEAEKRDNRRLGKELDLFSTHEEAGPGLIYWHPKGGRFRVELEKWWR
                     DEHYANGYDILFTPHVGKSWLWETSGHLGFYKEGMYAPMEIDEDKYYIKPMNCPFHIM
                     IYNSNNHSYRDLPLRWAELGTVYRYERAGSLHGLMRVRGFTQDDAHIICTPEQIED"
BASE COUNT          577 a         1510 c         1377 g          684 t
ORIGIN      
        1 ttgccgacgc cggcgtcgcc gacgtggatc gggttgttct tgagcctgcg gcagaggacc
       61 tggacggtgc gctcgatctc cgccgtccgg ccgatgacgg gctcgagctt gccctcgcgg
      121 gcgagtttcg tgaggtcgct cgtgaagcgc tccagggggc cgacgcggcc gcccttctgg
      181 ggccgccgct cctcgcgctc ctcgtggccc tcctcctcgt cctcgtcctc ctcctcgtcc
      241 tcctcgtagg cgtcgccgcc ctcctcggag ccgtgggaga tgacctccag gagctggagg
      301 cgcttcactc ccgccttcct taagtagtag gcggaatagt tgcgctcctc gtcgaagagg
      361 gagacgagga tgtccgccac ctcgacctcc tccttgcccg cggcctggga ctggaggacg
      421 gcgcgctcga tcacgctctg gaagccggcc gtctgcacgg gctctgcgtt cttcacctgc
      481 gggatcttct gctcgaagta ggcctccatg ccccgcttga gctggtccac gtccgcctcg
      541 caggcggcca ggatggagcg gacctcctcg aaggagagcg atgcgtagag gatgtgctcc
      601 ggcgtgaggt actcgtggtt gcggagcttg gcctcgttgt acgcggcgtt gaatatagcc
      661 tggacttcct ggctgacctt catgtcctat gccttctcca tcgtgcactt gagcggaaag
      721 ccgttctggc gggcgagctc gtgcacctga ttgatcttcg tcgcggcgat gtcgtaggga
      781 taggtcccca cgacgccctt ccccttccgg tggacgtcga gcatgatcct cgtcgcctcc
      841 ggcagggact tccggaacac cgtcatgagc accgtcacga cgaattccat ggtcgtatag
      901 tcgtcgttca ggaggaggat ccggtactcg tccggctcct tgagctcctc ctcctcgcgg
      961 gcgagcgtcg ccccttcgtt ctgcggttca tttgccatgg ttgcttctag actaaaaata
     1021 cagactaggc ggactaaaaa caagcccggc gggcgagcgg ggcccgccgg ggcctgagcc
     1081 gagggcccgt aggccgcgct tgccgaggct gcggggccct cgccgcgccg cctcgcgccc
     1141 gattcgcgcg ctttccgctc caacagggcc gtcgttccgg cgtactataa ggcatggaag
     1201 aggaggtgat cgtcgacgcc cgcgccctcc gggaggacgc gctctccggg ggggagaggg
     1261 ccgcccgcgc cttcggccga ctctggtccg tctattatcg ccgactttcg gcctacgcgg
     1321 cctccttccg gggcctgccc cggggcgaga gggaggacgc ggtcgccgag gccctggtgt
     1381 cggccttttc ccgcctcgaa tcctacgacc ccggtcgccc gctctcctcc tgggtctacg
     1441 ccattgccca caaccgtttc gccgacgagg cgcggcgcgc ccggcgcctt tcccccgtgg
     1501 aggtggaggc ggcggccggg cccagggagg gggaggggcc gccggacggc gcccacgcgg
     1561 aggcgatcgt ctcccggctc tccgacgccg agctcgaggc ggcctgcgcg gccgcgattg
     1621 ccgagctccc cgagctcgac cgccgcgtcg ccgccttgag cttcctggag ggcctcgact
     1681 cggccgaggt cgggcgcgtc ctgggcatgg cgccgggaac cgtgcgctgg cgcatccatc
     1741 gcatccgcgc ccgggtcgcc gggcgagtgg gagggagacc gtgacgaggc tggaccgggc
     1801 cctgaaagag gcgctccgcc gcgaggccga ggccgcggac tataggccgc cgagcggggc
     1861 ggagctggcc agggccctcg gaccgggccg agccgcggcc ttccgcgcgg tcccgtccgc
     1921 gtccgcgcgg aaggccgcca gcctggcggc gggcttcctg gccctctacg cctcgggctt
     1981 cgcggccggc ctcgcgctct cgatctggct gaaggcgccg gacgccgcgg ccctgatagg
     2041 tcgctccctg ccggcgaagc cgggggaagg gctctaccgc ttcatcgtcg aactgcgccc
     2101 cggctggccg ggaggcgccg gccggcgggg cgcctcggcc ccgccgctcg aataaggagg
     2161 ttcccgtgct caaaagactg cttctgcccc tctccgtcgc cttcctcgcc gtcgtcggac
     2221 tcgtcctcct ctggggagga ggcctctggc tcttcctcga cggctacgcc ctcgtcctgg
     2281 tgcccggact gggtaccctc tacgccttcg cgatgttcgg cggccgggcc ggcgcgggag
     2341 ccttcgccgc ggccctggac gaaggggctt ctccctccga cctccgctcg gcccaggcct
     2401 tcttcgacgc cctgggccgc gcctatctgg ccttcgcctt cctcggcgcc atcgtgagct
     2461 tcatcgacat gctcaagaac ctcgccgacc ggacgcagct cggtccccgc ctcgccgtgg
     2521 cctgcgtctc cctcgagtac gccgccatcc tcctcgtctt ccttgtgctg cccttccgca
     2581 cggccgcggc ccggcgcctg gcggcggagg aggagggggg ccggccgcgc gcttgacagc
     2641 gcccgccgtc gcgggtattt ttgccctaca ggctttgatg gagaagagta gccgaaagcc
     2701 ccgccacagg gagccggacc cgcgattgag aggtccaggc ggcggaagag gcggcgaaaa
     2761 ccactcctga gccgtccggg tgaaagcgcg ttccgcgcgg ggcctccagg ttccgcggcc
     2821 tgaggcgcga gtattccgga ccggcgcgcc gccgtcccgt aaaaggaagg cggcgcggtc
     2881 gagggaagcc ccgcgggcgt cgcccgggga gccctccttc gattgccgtc cgtcaaacgg
     2941 cgaagagcgc ggcccccgtc atggggcgcc gaagtaaggt ggtaccgcgg atttccgggg
     3001 ctccctcggg aattcgtcct tacgcgaagg cgaggagcct ttccgtcgag agggcgaatt
     3061 cccgacggag ccccgtcttt tatccggccg agcctggccg ccgtttcggg ccgcggagtc
     3121 gacgccgcgg gacgccgaga aggagtaccc catgtcatcg aacgtcgaga ccatccgcca
     3181 ttccgcgagc cacgtcctcg cggaggccgt cctcaggctc taccccggca ccaagttcgg
     3241 gataggcccc gcgatagagg acggcttcta ctacgacttc cagctccccg cgcccatcca
     3301 gcccgaggac ctgcccgcga tcgagaagga gatgcgccgc atcatcgcct cgggcgccga
     3361 gttcgtccgc agcgaggtgt cgaaggagga ggcgaggagg cgcttcgccg accagccctt
     3421 caagctcgag ctcatcgacg gcctggagga gggctcgatc tccctctacg agcaggacgg
     3481 cttcgtcgac ctctgccgcg ggccccacgt cgcgaccacc cgcgagatcc ggcccgacgc
     3541 cttcaagctc cgcgccgtcg cgggcgccta ctggcgcggc gacgagaagc gccccatgct
     3601 cacccgcatc tacgcctacg ccttcgcctc caaggccgac ctcgaggccc acctcaagat
     3661 gctcgaggag gcggagaagc gcgacaaccg ccgcctcggc aaggagctcg acctcttctc
     3721 cacccacgag gaggcgggcc cgggactcat ctactggcac ccgaagggcg gccgcttccg
     3781 cgtcgagctc gagaagtggt ggcgcgacga gcactacgcc aacggctacg acatcctctt
     3841 caccccccac gtcggcaagt cctggctctg ggagacctcc ggccacctcg gcttctacaa
     3901 ggagggcatg tacgcgccga tggagatcga cgaggacaag tactacatca agccgatgaa
     3961 ctgtcccttc cacatcatga tctacaacag caacaaccat tcctaccgcg acctgcccct
     4021 gcgctgggcc gagctcggca ccgtctaccg ctacgagcgc gccgggtccc tccacggcct
     4081 catgcgcgtc cggggcttca cccaggacga cgcccacatc atctgcaccc cggagcagat
     4141 cgaggacg
//
LOCUS       DAOOSJ010000235         4093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13869_length_4093_cov_4.339772, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000235 DAOOSJ010000000
VERSION     DAOOSJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13869_length_4093_cov_4.339772"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1316)
                     /locus_tag="PLG14_10465"
     CDS             complement(84..1316)
                     /locus_tag="PLG14_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOX49597.1"
                     /translation="MKIWMKYLIGALLGAAIALVVPAGDAALKATLGFLFDLSIRIGR
                     YALVPLILFSVPVAVFELNEDKEFWRVLGRIMLVLLASVAAFALIGVATASLVRPARI
                     PLLADAAPSASTLDLKGLLLAVFPESLFSIFASSGSFLLPVYFFAVLLGLAFSFDKTA
                     TRPVLSLVDSLSRIFYQINSFFAEFLGVLVITATAYNVLKFRSELRVDVYGPLLLLVG
                     IEVLVLALGVIPAVLYFAGGKKNPYRVLYALLAPSLAALVSGDVYFPLGSLMKHAKES
                     LGIRRRINGLVLPLAVLFGRAGTALVTTTAFIVVLSSYSGLGISIGSLAWILAAAPLL
                     TLLLGASPGRGAMTALTALCALYGRGFENGYLIVAPIALPLVAAGAFLDTLWAGAAAV
                     LVAKKTGQMQEKEARFYI"
     gene            1440..2705
                     /locus_tag="PLG14_10470"
     CDS             1440..2705
                     /locus_tag="PLG14_10470"
                     /inference="COORDINATES: protein motif:HMM:NF026171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49598.1"
                     /translation="MKFESTRRGGQALPFREAVLRGYPADGGLFMPAGEADLRYAVYS
                     PAASFQETIALAAGALFPEELDPLKAAAVADLAFASEPSAFAYGEDLLVLDLARGPSA
                     SAADYGAAFAAAITGDLLAAPGVAVAAAEGREAAALAAAFAGPKGQASRRGAAPLVLL
                     CPEEGLRGLPPSIRGGGVILLGVKGGPEAASLLERRASGGRLGAASLIPLGAGTPARL
                     AGRALLLVGLFALARRGLSGDLLVSAPPSDGFGLATGLWAWSWGLPVSAFVVARPPSA
                     PRLPGDSLAGFGLDPMAEELLAPFARDCPLGSLALLVDVEAGAARREAAALAASGGPG
                     LDAGSALGLAAARRALAAGFGGHARIVVPRFADPSWDAPPSGAAEEKAEGALAGAAGG
                     QPGGAELLEPDALVEPDLDSLVHALSRLL"
     gene            2769..>4093
                     /locus_tag="PLG14_10475"
     CDS             2769..>4093
                     /locus_tag="PLG14_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX49599.1"
                     /translation="MEKASLPGTAPTDRGIYGSLLRIALPAMAGSFVETLYNLTDAFF
                     LGKLGAAEISAPSIAFPILFFLIVFGNGLSGAGTTLIAQSKGRGDPEKMNGYLNQMVF
                     FLGLASLLFMLLGLASALPLLKLLKTPAEVLGPAHTYLSIALLGMPFMFAYFSLQAGF
                     MALGDSFTPLRVHLLAVGVNVILDPLLIFGLGPLPRLGVAGAAIATVASQGVAAFLSV
                     AILVRGRYGLRLSRKAMRPRAEAFRLFMRIGLPSSVGQGLSALGFTVLQGVVNFFGAS
                     AIAAFGVGNRIISLFDIPAHGIAGAATSLTGQALGAGDHRRAKKVVKAALVACLLLLA
                     PPLCASFFWGGGLVRFFVDDPEAIRLGGVMFKIVSPSVLLFGLYLVTTGAFQGAGATR
                     IIMVLAIARLWLIRVPLASFLAFRLGTGPVSIWYAMFVSNAATALAGFL"
BASE COUNT          551 a         1484 c         1418 g          640 t
ORIGIN      
        1 gttcttgagg ttcatggtct tattctacgg ctcgactcgc cgcggcgtca agctggaacg
       61 gcgtttctgg agaaattgcc gtttcatatg tagaaacggg cctctttctc ctgcatctgc
      121 ccggtctttt tcgccaccag gacggcggcg gcccccgccc aaagggtgtc gaggaaggct
      181 ccggccgcga cgagggggag ggctatcggc gcgacgatga ggtagccgtt ctcgaagccc
      241 cttccgtaga gggcgcagag ggcggtgagg gcggtcatcg cgccgcgtcc cggggaggcg
      301 ccaaggagga gggtgaggag gggggcggcg gcgaggatcc aggccaggct cccgatggag
      361 atgccgaggc ccgagtagga ggagaggacg acgatgaagg cggtggtcgt gaccagggcc
      421 gtgccggcgc ggccgaagag gacggcgagg gggaggacga ggccgttgat cctccgcctg
      481 atccccaggc tctccttcgc gtgcttcatg agggagccca ggggaaagta gacgtcgccg
      541 gagaccaggg ccgcgaggga gggcgcgagg agggcgtaga ggacgcggta ggggttcttc
      601 ttccccccgg cgaaatacag gacggcgggg atgactccca gggccaagac caggacctcg
      661 attccgacga ggagaaggag ggggccgtag acgtccacgc gcagctccga gcggaatttg
      721 aggacgttgt aggccgtcgc cgtgatcacg aggacgccca ggaactcggc gaagaaggag
      781 ttgatctggt agaagatgcg cgagagggag tcgacgaggg agaggacggg cctggtcgcg
      841 gtcttgtcga aggagaaggc gaggccgagg aggacggcga agaagtagac ggggaggagg
      901 aagctccccg aggaggcgaa gatggagaag aggctctccg ggaagacggc gaggaggagg
      961 cccttgaggt cgagggtcga ggcggagggc gcggcgtcgg cgaggagggg gatgcgcgcc
     1021 ggccggacca aggaggccgt cgccacgccg atgagggcga aggccgcgac ggaggcgagg
     1081 aggacgagca tgatcctccc gaggacgcgc cagaactcct tgtcctcgtt gagctcgaag
     1141 accgcgacgg gcacggagaa gaggatgagg gggaccaggg cgtagcggcc gatcctgatg
     1201 gagagatcga agaggaagcc gagggtggct ttgagggcag cgtctcccgc cggcacgacg
     1261 agggcgatgg ccgccccgag gagggcgccg atcaggtact tcatccagat cttcatgggg
     1321 gcagagcata gcctccgcgc cattggtcgt caatcgcgcc cgccttggcc gaggcctggg
     1381 ccgggccctg cccgcccgcc ttgctacgtc cgggcctcgc ggatagactc tcaggcacga
     1441 tgaagttcga gagcacgagg aggggcgggc aggcccttcc cttccgcgag gcggtcttgc
     1501 ggggctatcc cgccgacgga gggctcttca tgcccgcggg ggaggccgat ctccgctacg
     1561 ccgtctattc gcccgccgcc tccttccaag agaccatagc cctcgcggcc ggcgcccttt
     1621 tccccgagga gctcgatcct ttgaaggcgg ctgccgtggc cgacctcgcc ttcgcctccg
     1681 agccctccgc cttcgcctac ggcgaggacc tcctcgtcct cgacctggcc aggggcccct
     1741 ccgcctccgc cgccgactac ggagccgcct tcgccgcggc cataacgggc gaccttctcg
     1801 ccgcgccggg cgtcgccgtc gccgcggccg aaggccggga agcggccgcc ctggccgcgg
     1861 ccttcgccgg cccgaagggc caggcttccc gccgcggcgc cgcgcccctc gtcctcctct
     1921 gccccgagga ggggctccgc ggccttccgc cctcgatccg gggcggcggc gtcatcctcc
     1981 tcggcgtcaa gggcggcccg gaagccgcca gcctccttga gcggagggcc tcaggcgggc
     2041 ggctcggagc cgcctccctc atccccctcg gggcgggaac cccggccagg ctcgccggca
     2101 gggccctcct cctcgtcggc ctcttcgccc tcgcgaggag gggcctttcg ggcgacctcc
     2161 tcgtctcggc ccccccgagc gacggcttcg gcctcgcgac gggcctctgg gcctggagct
     2221 ggggcctccc ggtgagcgcc ttcgtcgtcg cccgcccgcc ctccgcgccc cgcctccccg
     2281 gcgacagcct cgcgggcttc ggcctcgatc ccatggccga ggagctcctc gcccccttcg
     2341 cccgcgactg tcccctgggt tccctcgccc tcctcgtcga cgtggaggcg ggcgccgcgc
     2401 gcagggaggc cgccgccctc gccgcctcag gcgggcccgg cctggacgcg ggctcggccc
     2461 tcggcctggc cgcggcgcgc cgcgccctcg ccgcgggctt cggcggtcac gcgcggatcg
     2521 tcgtgccgcg cttcgccgat ccctcctggg acgccccgcc ttctggcgcc gccgaggaga
     2581 aggcggaagg cgcgctcgcc ggcgcggccg gcggccagcc cggaggcgcg gagctcctcg
     2641 aaccggacgc cctggtcgag ccggacctcg attccctggt ccacgccctt tcccgtcttc
     2701 tctagagccc ctggccgttg cccctcggcc gttgcccctc ggccgcgaag cctgctatgg
     2761 tgtccggcat ggaaaaagcc agccttccgg gaaccgcgcc gaccgacagg ggcatctacg
     2821 gcagtcttct caggatagcc cttccggcga tggcgggaag cttcgtggag actctctaca
     2881 acctcacgga cgccttcttc ctgggcaagc tcggcgccgc ggagatctcg gccccctcca
     2941 tcgccttccc gatactcttc ttcctcatcg tcttcgggaa cggcctctcc ggcgcgggca
     3001 cgaccctcat agcccagtcg aagggcaggg gggaccccga gaagatgaac ggctacctga
     3061 accagatggt cttcttcctc ggcctggcct cgctgctctt catgctgctc ggcctggcct
     3121 cggccctgcc cctcctcaag ctcctcaaga cccccgccga ggtcctcggg ccggcccaca
     3181 cctacctctc catcgccctc ctggggatgc ccttcatgtt cgcctacttt tccctgcagg
     3241 ccggcttcat ggccctgggc gacagcttca cgccccttcg ggtccacctc ctggcggtcg
     3301 gcgtcaacgt catcctcgac ccgctcctca tcttcggcct gggccccctg ccgcggctcg
     3361 gggtcgcggg cgcggccatc gcgaccgtcg cctcccaggg cgtcgccgcc ttcctctccg
     3421 tcgccatcct ggtccggggc cgctacggcc tcaggctctc gcggaaggcg atgcgccccc
     3481 gcgcggaggc cttcaggctg ttcatgcgga tcggcctacc ctcgtcggtc gggcagggcc
     3541 tctccgccct gggcttcacc gtcctccagg gagtcgtcaa cttcttcggc gcctccgcga
     3601 tagccgcctt cggcgtcggc aaccgcatca tcagcctctt cgacatcccg gcccacggca
     3661 tcgcgggcgc ggcgacgagc ctcacgggcc aggccctggg ggcgggcgac caccgccggg
     3721 cgaagaaggt cgtgaaggcg gccctcgtgg cctgcctcct cctcctcgct ccgcccctct
     3781 gcgcctcctt cttctgggga ggaggcctcg tgcgcttctt cgtcgacgac cccgaggcca
     3841 tcaggctggg cggcgtcatg ttcaagatcg tgagcccctc cgtcctcctc ttcggcctct
     3901 acctcgtgac gacgggcgcc ttccagggcg ccggggcgac caggatcatc atggttctcg
     3961 cgatagcccg gctctggctc atccgcgtgc ccctcgcctc cttcctcgcc ttcaggctcg
     4021 ggacgggacc cgtctccatc tggtacgcga tgttcgtctc caacgcggcg acggccctgg
     4081 ccggcttcct cta
//
LOCUS       DAOOSJ010000236         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13900_length_4085_cov_5.925806, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000236 DAOOSJ010000000
VERSION     DAOOSJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13900_length_4085_cov_5.925806"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..934
                     /gene="mazG"
                     /locus_tag="PLG14_10480"
     CDS             56..934
                     /gene="mazG"
                     /locus_tag="PLG14_10480"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOX49600.1"
                     /translation="MSPTAAGPAPADGAAAPGPGAAGSGAAGAAFQELYRIVARLRAP
                     DGCPWDREQTPASLRGGLVEETYELIEAIDEGDAEHVREEAGDLFLLATMIAYMHEEE
                     GGFSVADALGGIAEKLVRRHPHVFGESDADTSEKVLAQWKEIKEKVEGRRPKDSVLDE
                     VSRALPPLERAYKLQKKAAKAGFDWPRSEEVWAKAREELAEAEEACEAAAREGDREAL
                     EGELGDLLFSVVNLARWLKIDPALALNRTADKFSRRFRHVERRMAESGKALGPESMGL
                     MDEFWNEAKAAEDTKG"
     gene            complement(940..1707)
                     /locus_tag="PLG14_10485"
     CDS             complement(940..1707)
                     /locus_tag="PLG14_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49601.1"
                     /translation="MIRVGLSSQALLTRSPSEVLALAKAAGLDGVEWAGEAHVPTGDG
                     AAQDRLLMDTLRAGLTVASIASLYRVTPGAEQGLGFESLLAGAVRLQAPVLRVYAGAL
                     PAARLSPAEREALRGELSRLGDLAGKRGVTICLSLGRNTSLDNYAAAIGVVEGAAHPF
                     VRLAWEPLPGVEAAKATAALESAARGVGLLLAKRSDRDGKGGPLSEEGEAWRLRLEAY
                     RRGEGDPAMGRFVLLGRVGEDDEGRLREDAAFLRSLA"
     gene            1870..2463
                     /locus_tag="PLG14_10490"
     CDS             1870..2463
                     /locus_tag="PLG14_10490"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49602.1"
                     /translation="MPKIVDYDERRSEIATKAVAVFVREGFHESNLSKIAELCGFGRT
                     TIYKYFKNKEEIFVYTIEDVFGRLDRLTSSVIDDRELKPADKLEKLLGLLVRKAVEEK
                     DRMVLILDLLLHPDREDKSSRLNASEQVLKLRAAIERIIAEGIEAGELKRVNPEAMAF
                     TLFSLVESSTMHNSLYSHFSLEETLEAAKALLASLRA"
     gene            2624..>4085
                     /locus_tag="PLG14_10495"
     CDS             2624..>4085
                     /locus_tag="PLG14_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX49603.1"
                     /translation="MKDLTQGNETKAIVFFALPMLLGNIFQQFYNMVDSFVVGRFVGT
                     AALAAVGTGFPIIFLMIALIMGVTMGSTVLVAQYFGAKEYAKVRAAVDTAYVFLFWAG
                     LALSVLGVAFTEPILRLLRVPADVFPEASVYLRIIFAGMLPSFGYNAVSAILRGLGDS
                     KTPLYLLVVSTVINIALDLSFVVLFHWGVAGVAWATVIAQTASFAGAIVVLNRRNEFL
                     RLKLKELTFDREVFGLSLKIGLPTGLQQTLVAAGMMALTRIVNGFGTSTMAAYAAASR
                     LDSFASMPAMSLGAAATTFVGQNLGARKPERVKRGHLSAVLLGGAYSLLTAAAVILFG
                     RALMGIFSADAEVIAIGARYLFIVGLFYVLFSTMFITNGALRGAGAAMVPMFNTILAL
                     WVVRIPLALLFSGPLGMGSDGIWWSIPAGWAVGATMATAYYASGKWKGKAIAGRGPAG
                     SGPAGGAGPGDSQAAAAEPAQAAARRGAAAPRGPASE"
BASE COUNT          602 a         1527 c         1358 g          598 t
ORIGIN      
        1 cgagggccgc gggggcgcgg cgtcgcccgc agtcggcgcg accgcgcccg gcgcgatgtc
       61 gcccaccgcg gcgggcccgg cgcccgccga cggcgccgcg gcgcccggtc ccggagccgc
      121 cggctcaggc gcggcggggg ccgccttcca ggagctctac cgcatcgtcg cgcggctccg
      181 cgcccccgac ggctgcccct gggaccgcga gcagacgccc gcgagcctcc gcggcggcct
      241 cgtcgaggag acctacgagc tcatcgaggc catcgacgag ggcgacgcgg agcacgtccg
      301 cgaggaggcg ggcgacctct tcctcctcgc gacgatgatc gcctacatgc acgaggagga
      361 ggggggcttc agcgtcgccg acgcgttggg cggcatcgcc gagaagctcg tgcggcgcca
      421 tccccacgtc ttcggcgaga gcgacgcgga cacctcggag aaggtcctgg cgcagtggaa
      481 ggagatcaag gagaaggtcg agggccggcg gcccaaggac tcggtcctgg acgaggtgtc
      541 ccgcgccctt ccgcccctgg agcgggccta caagctccag aagaaggcgg cgaaggccgg
      601 cttcgactgg ccgaggtccg aggaggtctg ggccaaggcc cgcgaggagc tcgcggaggc
      661 tgaggaggcc tgcgaggccg ccgcccgcga gggcgaccgg gaggccctgg agggcgagct
      721 cggcgacctc ctcttctccg tggtcaacct ggcccgctgg ctcaagatcg atcccgccct
      781 ggccctgaac cggaccgccg acaagttctc gcggcgcttc cgccacgtcg agcgccgcat
      841 ggccgagtcg ggcaaggccc tcggccccga gtcgatgggc ctcatggacg agttctggaa
      901 cgaagccaag gccgccgagg acacgaaggg ctgaaggccc taggcgaggg atcgcaggaa
      961 ggccgcgtcc tcgcggaggc ggccctcgtc gtcctcgcca accctcccca gcagcacgaa
     1021 acggcccatc gcgggatcgc cctcgccgcg cctgtaggcc tcgagcctca ggcgccaggc
     1081 ctcgccctct tcggagagcg gcccgccctt cccgtcccgg tcgctccgct tggcgaggag
     1141 gaggccgacg cccctcgcgg cggactccag ggcggccgtc gccttggccg cctcgacgcc
     1201 gggcaggggc tcccaggcca gtcggacgaa gggatgggcc gcgccctcga ccacgccgat
     1261 cgcggccgca tagttgtcca ggctcgtgtt cctccccagg gaaagacaga tcgtcacgcc
     1321 ccgcttgccg gccaggtcgc cgagccgcga aagctcgccg cgcagggcct cccgctccgc
     1381 gggcgaaagc cgcgccgcgg gcagggcgcc ggcgtagacg cggaggacgg gcgcctggag
     1441 gcggaccgcg ccggcgagga gggactcgaa gccgaggccc tgctccgcgc cgggggtcac
     1501 gcggtagagc gaggcgatgg aggccaccgt gaggccggcg cgcagggtgt ccatgaggag
     1561 gcggtcctgg gccgccccgt cccccgtcgg gacgtgggcc tcccccgccc attcgacgcc
     1621 gtcgaggccg gcggccttgg ccagggccag gacctcggag gggcttctcg tgaggagggc
     1681 ttgcgaggac agtccgacgc ggatcatggc ggctcccgga ggcggcccct gcccaaaggc
     1741 gggcggccgg tatttttact cgttggaaga cgatcatacc cgagccgccc gccttcctca
     1801 agcgactcct ttttattgac atatttgtca tctttgagta tcatcgcccg accccaagca
     1861 ggagacggca tgccgaagat agtcgactac gacgagcgga gaagcgagat agccaccaag
     1921 gcggtcgcgg tcttcgtccg cgagggcttc cacgaatcga acctctccaa gatcgccgag
     1981 ctctgcggct tcggaaggac gacgatctac aagtacttca agaacaagga agagatcttc
     2041 gtctacacga tcgaggacgt cttcggccgg ctcgacaggc tcacgagctc cgtgatcgac
     2101 gaccgggagc tcaagcccgc ggacaagctc gagaagctcc tcggcctcct tgtccggaag
     2161 gccgtggagg agaaggaccg catggtcctc atcctcgacc tactcctcca ccccgaccgg
     2221 gaggacaaga gctcccggct gaacgcgagc gagcaggtcc tgaagctccg cgccgccatc
     2281 gagcggatca tcgccgaggg catcgaggcc ggcgagctca agcgcgtcaa tcccgaggcc
     2341 atggccttca ccctcttctc cctcgtcgag tcctccacga tgcacaactc gctctacagc
     2401 cacttttccc tggaggagac cctggaggcc gccaaggccc tgctcgccag cctcagggcc
     2461 tagggccggc cgccgcgccc cgtccctttg ccgcggcagc ccaagccgcg cggtcggccg
     2521 ggcgggccgc ctggaggcgc ggcttagcgg gccggcttcc tccttgtccc cagcccgggt
     2581 tttcgctacc ctggacgaaa gccccaggca cgcaaggatc gccatgaaag acctcaccca
     2641 ggggaacgag acgaaggcca tcgtcttctt cgcccttccc atgctcctcg gcaacatctt
     2701 ccagcagttc tacaacatgg tcgacagctt cgtcgtcggc cgcttcgtcg gcacggcagc
     2761 cctggccgcg gtcgggacgg gcttccccat catcttcctg atgatcgccc tcatcatggg
     2821 cgtgacgatg ggctccacgg tcctcgtcgc ccagtatttc ggcgccaagg aatacgccaa
     2881 ggtccgcgcc gccgtcgaca cggcctacgt cttcctcttc tgggccggcc tggccctgag
     2941 cgtcctgggc gtcgccttca cggagcccat cctccgcctc ctcagggtcc ccgcggacgt
     3001 cttcccggag gcctcggtct acctccggat catcttcgcg ggcatgctcc cctccttcgg
     3061 ctacaacgcc gtctcggcca tcctgcgcgg cctcggcgac tcgaagacgc ccctctatct
     3121 cctcgtcgtc tcaaccgtca tcaacatcgc tctggacctg tccttcgtcg tcctcttcca
     3181 ctggggcgtc gcgggcgtcg cctgggcgac ggtgatcgcg cagacggcct ccttcgccgg
     3241 cgccatcgtc gtcctcaacc ggaggaacga gttcctccgc ctcaagctca aggagctcac
     3301 cttcgaccgc gaggtcttcg gcctctccct caagataggc ctcccgaccg gcctgcagca
     3361 gaccctggtc gccgccggca tgatggccct cacgcgcatc gtcaacggct tcggcacctc
     3421 gaccatggcg gcctacgccg cggcgagccg cctggactcc ttcgcctcca tgccggccat
     3481 gagcctgggc gcggcggcga cgaccttcgt agggcagaac ctgggcgcgc gcaagcccga
     3541 gcgggtgaag cgggggcacc tctccgccgt cctccttgga ggggcctact ccctcctcac
     3601 cgccgccgcc gtcatcctct tcggccgcgc cctcatgggc atcttctccg cggacgcgga
     3661 ggtgatcgcg ataggcgccc gctacctctt catcgtcggg ctcttctacg tcctcttctc
     3721 cacgatgttc atcaccaacg gcgccctccg cggcgccggc gccgcgatgg tgccgatgtt
     3781 caacacgatc ctcgccctct gggtggtccg catccccctg gccctcctct tctcggggcc
     3841 gctcgggatg ggctcggacg gcatctggtg gtccatcccc gcgggctggg ccgtcggcgc
     3901 aacgatggcg acagcctact acgcctccgg gaaatggaag ggcaaggcga tagccggcag
     3961 aggtcccgcg ggctccggcc ccgcgggcgg cgcaggaccc ggagactccc aggcggcggc
     4021 cgcggagccc gcccaggcgg ccgcccggcg cggcgccgcg gcgccgcgcg gccccgcctc
     4081 cgagt
//
LOCUS       DAOOSJ010000237         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13915_length_4082_cov_4.583809, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000237 DAOOSJ010000000
VERSION     DAOOSJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13915_length_4082_cov_4.583809"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLG14_10500"
     CDS             complement(<1..466)
                     /locus_tag="PLG14_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX49604.1"
                     /translation="MRQRRVLVVDDERNMQAVLKLLLEGEGYETACVADGAEALALLE
                     AGERFGAVVSDLKMDRLDGIGLLRAIHERGLAVPFVLITAYGTIEKAVEAMKLGAVDV
                     VTKPFTKEILLNILSRIFRLTCLEDENAILRSALRQDELVYRSPAMAEIVRLL"
     gene            complement(463..2157)
                     /locus_tag="PLG14_10505"
     CDS             complement(463..2157)
                     /locus_tag="PLG14_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX49605.1"
                     /translation="MAFAKTRKISTKVMLSFALIVLFQGALSILTLSYVTNRAMQSSL
                     EDQRRKTRLLIEKYFTDARKEMIVKADLLAGQAKIVDFVEGGQDGMLWRELSFYLAPL
                     KLDTILVIDERGEKVAAAGNSEIADVLLKRNPLDLLAQGSLTTIAGEGNRIHLWGLHA
                     IGAPGNPKGMIGVATSLDRSFIGRVEEISSTQMLLTLRKTIPVNGRVSDNVFIEYNRR
                     VAADPLAGQAGKIGAYVYRTTYLPEYPELEMVYFIDTAPSIRLMSNYVASTLAILAIA
                     LLVAFWISVLLYRYSFLKPFAAFQKAIREISRGDLSFRFSRDAADEFADLEREFENMT
                     VELKKLESKLQISSRMAAIGEMVAGVAHQIRNPLAIMKVSAEMIRDGLPPERAKPLAD
                     MMVSEVDSLSAVVTKFLDFTRPLKVNRQEVELGDFLARTVSLLPLERYPGRRVEVDAG
                     PGPLRASLDPHLMEQALQNLLVNALEASKEGQAVRLAAGREDGLLRLRVADSGCGMAE
                     EVRSQLFHPFFTTKSDGTGLGLSIVHRIVEAHGGLVEARSEPGAGTEFAITLGEGA"
     gene            2448..3839
                     /locus_tag="PLG14_10510"
     CDS             2448..3839
                     /locus_tag="PLG14_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOX49606.1"
                     /translation="MSNLLSSLGAAFSAFFGFKAYVILPVFMLVLALAARMRPKEAVL
                     ASLRIAAGFSGIFIVFNFFVGQIGPAIKAIIALRGLDYPVVDVGWPPLAAITWSSFIA
                     PLCIPLVILLNLGMLLARATKTLYIDLWNYWHFAFLGALLMSATGSVPVALAGTLAIA
                     AYNIKTCEWTRPWVKRETGLEGIAISPLSVAGLLPYAVAMDRLLGAVPGLRRLDWNPS
                     RARKGGKAAGLLEEPMAIGFLVGLFLSVFAGYGLKETLETSVNIAAVMFVLPRCGGLI
                     GEGMGAVSLAVKTLVERRFAGMRGLSIAVDTGVLLGNPSVTATGLVLIPVSLLLAFVL
                     PGNRVIPLGDLPNLISIMSVTTIAMGGNVVRAVLAGIPVVATFMLFSSALAPVITRLS
                     AQAGLAAAAAGQEITAFTDGGSQVRYWMYWLFQGNLAALALAPAVAALLWYTWKRHKA
                     LSLDEESGGTGAR"
BASE COUNT          575 a         1400 c         1432 g          675 t
ORIGIN      
        1 ccaggaggcg cacgatctcc gccatcgccg gcgagcggta gacgagctcg tcctggcgga
       61 gcgccgaccg gagtatggcg ttctcgtcct ccaggcaggt gaggcggaag atccgcgaca
      121 ggatgttgag gaggatctcc ttcgtgaagg gcttcgtgac cacgtccacc gcgccgagct
      181 tcatcgcctc cacggccttc tcgatggtgc cgtaggcggt gatgaggacg aaggggacgg
      241 cgaggccgcg ctcgtggatg gcccggagaa ggccgatccc gtcaagcctg tccatcttga
      301 ggtcggagac gacggcgccg aagcgctcgc cggcctccag gagggcgagg gcctcggccc
      361 cgtccgcgac gcaggccgtc tcgtagccct cgccctcgag gaggagcttg agcacggcct
      421 gcatgttcct ctcgtcgtcg acgacgagca cccgtctctg cctcatgcgc cctccccgag
      481 cgtgatcgcg aactccgtcc cggccccggg ctcggacctc gcctccacga ggccgccgtg
      541 cgcctcgacg atcctgtgga cgatggagag ccccaggccc gtcccgtcgc tcttcgtggt
      601 gaagaagggg tggaagagct gcgagcgcac ctcctccgcc atgccgcagc ccgagtcggc
      661 cacgcggagc ctgaggaggc cgtcctccct ccccgcggcc aggcgcaccg cctggccctc
      721 cttggaggcc tctagggcgt tcacgaggag gttctgcagg gcctgctcca tgaggtgggg
      781 gtccaggctc gcgcgcaagg ggcccgggcc ggcgtcgacc tccacgcgcc tccccggata
      841 gcgctccagg gggaggaggg agaccgtgcg ggccaggaag tccccgagct ccacctcctg
      901 gcggttcacc ttgagcggcc tcgtgaagtc caggaacttg gtgaccaccg cggagaggga
      961 gtccacctcg ctcaccatca tgtccgcgag gggcttggcc cgctccggcg ggagcccgtc
     1021 gcggatcatc tccgcggaga ccttcatgat cgcgagaggg ttgcggatct ggtgggcgac
     1081 gcccgcgacc atctcgccga tcgcggccat ccgggagctg atctggagct tggactcgag
     1141 cttcttgagc tcgaccgtca tgttctcgaa ctcgcgctcc aggtcggcga actcgtcggc
     1201 ggcgtcgcgc gagaagcgga aggagaggtc gccgcgggag atctcccgga tggccttctg
     1261 gaaggccgcg aagggcttga ggaagctgta gcggtagagg aggacgctga tccagaaggc
     1321 gacgaggagg gcgatcgcca ggatggccag ggtggaggcc acgtagttcg acatgaggcg
     1381 gatgctcggc gccgtgtcga tgaagtagac catctcgagc tcggggtatt ccggcaggta
     1441 ggtcgtgcgg tagacgtagg ccccgatctt gccggcttgt ccggccaggg ggtcggccgc
     1501 gacgcgccgg ttgtactcga tgaagacgtt gtcggagacg cgcccgttca cggggatggt
     1561 cttgcgcagg gtgaggagca tctgggtgct ggagatctcc tcgaccctgc cgatgaagga
     1621 gcggtccagg ctcgtcgcga cgcctatcat ccccttggga ttgcccggag cgcctatcgc
     1681 gtggaggccc cagaggtgga tccggttgcc ctctccggct atggtcgtga gggatccctg
     1741 ggccaggagg tcgagggggt tgcgcttgag gaggacgtcg gcgatctcgg agttgcccgc
     1801 ggccgcgacc ttctcgcccc gctcgtcgat gacgaggatg gtgtcgagct tgaggggcgc
     1861 gaggtagaag gagagctccc gccagagcat cccgtcctgg ccgccctcca cgaagtcgac
     1921 gatcttggcc tggcccgcga ggaggtcggc cttgacgatc atctccttcc gagcgtcggt
     1981 gaagtacttc tcgatgagga gcctcgtctt cctgcgctgg tcctcgagcg aggactgcat
     2041 ggcgcggttc gtgacgtagc tcaaggtgag gatggagagg gcgccctgga agaggacgat
     2101 tagggcgaag gagagcatta ccttggtgga gatcttcctc gtcttcgcga aggccatgct
     2161 cagaccgctc ctttgtaggc tcgcttccgc ccggcgagcc gcggcgccaa ggccgggccg
     2221 cgtcgctccg tgcgaggccg cgcggggtcg ggcggccggg ccagtatagc acggcctcca
     2281 atagcccggg aggccgaaag ggccgggagg ggctcggcgg gcccaggcgc agctcggcgg
     2341 gacgcggcgg ggccgcgggg tatggcgggg ccgcgggcgg ccgaacaaag ggccgcattc
     2401 gcggcggccg cctggaagcg ggcccgagtc ctgctatact tggtcgcatg agcaacctgc
     2461 tatcaagcct cggggcggcc ttctccgcct tcttcggctt caaggcctac gtcatcctcc
     2521 ccgtcttcat gctcgtcctc gccctcgccg ccaggatgcg gcccaaggag gccgtcctcg
     2581 cctccctgcg catcgccgcc ggcttctcgg gcatcttcat cgtcttcaac ttcttcgtcg
     2641 gccagatcgg ccccgcgatc aaggccatca tcgccctgcg cggcctcgac tatcccgtgg
     2701 tcgacgtggg ctggccgccg ctcgcggcga tcacctggtc ctccttcatc gcgcccctct
     2761 gcatccccct cgtcatcctc ctcaacctcg ggatgctcct cgcccgcgcg acgaagaccc
     2821 tctacatcga cctctggaac tactggcact tcgccttcct gggggccctc ctcatgagcg
     2881 cgacggggag cgtccccgtg gccctcgcgg gcaccctcgc catagccgcg tacaacatca
     2941 agacctgcga gtggacccgg ccctgggtga aaagggagac cggcctcgag ggcatcgcga
     3001 tcagccccct ctcggtcgcc ggcctcctgc cctacgccgt ggcgatggac aggctcctcg
     3061 gcgccgtacc cggcctgcgg cgcctggact ggaatccctc gcgggccagg aagggaggga
     3121 aggccgcggg cctcctcgag gagccgatgg ccatcggctt cctcgtgggc ctcttcctct
     3181 cggtcttcgc gggctacggc ctcaaggaga cgctcgagac ctcggtgaac atcgcggcgg
     3241 tcatgttcgt cctgccgcgc tgcggcggcc tcatcggcga gggcatgggc gcggtctcgc
     3301 tcgcggtgaa gaccctggtg gagcggcgct tcgccgggat gcggggactc tccatcgcgg
     3361 tggacacggg cgtgctcctc ggcaatccct ccgtcaccgc gaccggcctc gtcctcatcc
     3421 ccgtctccct cctcctcgcc ttcgtccttc caggcaaccg ggtcatcccc ctcggggacc
     3481 ttccgaacct catctccatc atgtcggtga cgaccatcgc gatgggaggc aacgtcgtgc
     3541 gggcggtcct ggccgggatc ccggtcgtcg ccaccttcat gctcttctcc tcggccctcg
     3601 cgcccgtcat cacgaggctc tccgcccagg cgggcctggc cgccgccgcg gcggggcagg
     3661 agatcaccgc cttcacggac ggcggcagcc aggtccgcta ctggatgtac tggctcttcc
     3721 agggaaacct tgcggccctg gccctcgcgc cggctgtggc ggccctcctt tggtacacgt
     3781 ggaagaggca caaggcgctt tcgcttgatg aggagtcggg cggaaccggc gcgcgttgac
     3841 gcggtcgcgg gcgcggcggc gcggccgcgg ccttgcgcgg cctttgggtg gcgcggactc
     3901 gggcggcccg gcgccccgcg tccgcggcgc cgccgcctaa tgtcccggcg cggcgccggg
     3961 caggggcata cggggaggca ggcccagggc gcgcatcccc aggaggatga ggacggaggc
     4021 ggcgagaagg gccaggaacc agagggccgt gatgcccacc catttcccga agcccacctt
     4081 cg
//
LOCUS       DAOOSJ010000238         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_13916_length_4082_cov_4.487708, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000238 DAOOSJ010000000
VERSION     DAOOSJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13916_length_4082_cov_4.487708"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLG14_10515"
     CDS             complement(<1..345)
                     /locus_tag="PLG14_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49607.1"
                     /translation="MKRNIAFAIVIALIFGGAAMPAIAQPTASFEQFKTAFVDFSDAL
                     AGTLNLNSTIGNTWSDGWIGGFPHFGAGLALGSTFAGKDASQALVSAIGQTLPSELEN
                     LGVPIPAVAATAK"
     gene            complement(420..1394)
                     /locus_tag="PLG14_10520"
     CDS             complement(420..1394)
                     /locus_tag="PLG14_10520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX49608.1"
                     /translation="MRGKSDAAAEPREAASPAGAAGFESLRPAYARSVAELVSARASV
                     SELSAAFAPDRLVGLSAEEAGKAARALVEAAKAGGMDPERALGAVLPKGGAVSALLAT
                     DRDRRDFLAAAARDAPATLRHAEAYAEIARLVPLMRSIRGLYGALLESAYDALATRKS
                     ASPGPEATGPVARRGEVYAPPKRGLDRAHPYALDVFFTRIERSPSGEIGPEILSVSSG
                     IVLAAADDWKGGGGPSAYRSGGLSPNSGNGVIVYDPAARRYYSYFHLSRVNVGPGELV
                     EKGKVLGRGGDTGANARKAGHGGHVHVEIFDASKDRALPALEIREIIF"
     gene            complement(1790..2620)
                     /locus_tag="PLG14_10525"
     CDS             complement(1790..2620)
                     /locus_tag="PLG14_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX49609.1"
                     /translation="MSGRPNRGYSYARRRPSGGADVPYSRDGGRAAQGDGAAPPAARR
                     VLPEDWRTRTYLALDFETTGLDPARERVVEIGALRFRFVEPCGQADLGPLFAAALGGE
                     ADYGGEAGREAADGGGAFRGAGPGGPGAERLTLGEEGSLSLLVNPGKPIPPVTTAIHG
                     ISDKDVEGEPAFAALAPALAELARGAVIIAHNAPFDLSFLRAELSRAGLPPAANEVLD
                     TRIVAKAAFPGLPSYRLVDLAARLGIGHARAHRALDDARACAELFLASALRLDGGKTR
                     "
     gene            complement(2617..>4082)
                     /locus_tag="PLG14_10530"
     CDS             complement(2617..>4082)
                     /locus_tag="PLG14_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOX49610.1"
                     /translation="VFAEMTARVFAANGVKAYLFSALRPTPELSYAIRELKADTGVVV
                     TASHNPPKYNGYKAYWNDGSQVTPPHDTGIIDMVNAVKAPKLMPKAEAMEKGLLKVID
                     REIDDPYVAMVKSKLLRPALIKELGKSVKIVYTPLHGTGALHFERIMGELGLEVVTVP
                     EQREPDGAFPTVAFPNPEETAALKLALELGAKVKADCVMATDPDADRLGIAVPDAKGD
                     FVLITGNQLGSLHLDYILLTRKELGLMPRRPAAIRSIVTTSLQDRIAKAYGAECRECL
                     TGFKWIAELMRKLEAEGVDFLYGTEESYGHLVEPEVRDKDGISAAALTAEMTLYWRSK
                     GKSLLDRLDELYLEHGYFEEKGVNKYFEGPAGMDIMRGIMEAYRKDQPAGFGGIKVAE
                     IRDVKTGRKWQAGRPEAAEKVELPASDVIQWYLEDGTVLTVRPSGTEPKIKYYILLRT
                     EVEGGKAAGPAALAAARAASAKKAAAIEADVRKVIDR"
BASE COUNT          554 a         1343 c         1540 g          645 t
ORIGIN      
        1 cttggccgtc gcggcgaccg cggggatggg cacgcccagg ttctcaagct cgctcggcag
       61 ggtctggccg atcgcggaga caagggcctg cgaggcgtcc ttgcctgcga aggtggagcc
      121 cagggcgagg cccgccccga agtgggggaa accgccgatc cagccgtcgg accaggtgtt
      181 gccgatggtg gagttgaggt tgagggtgcc cgccagggcg tcggagaaat cgacaaaggc
      241 cgtcttgaac tgttcgaaag acgcggtcgg ctgagctatt gccggcatgg ccgcaccgcc
      301 aaagatgagg gcgatgacta tggcgaaggc gatattccgt ttcatggtac ctcctgtggc
      361 gatactccca taagtatacc gccccgggtg gcgcttatcc aaaaagtcaa aatagataat
      421 taaaagatga tttccctgat ctccagggcg ggcagggcac gatccttgct tgcgtcgaag
      481 atctccacgt ggacgtgtcc gccgtggccg gccttccgcg cgttggcgcc cgtgtcgccg
      541 ccgcggccca gcaccttgcc cttctcgaca agttcgccgg ggccgacgtt gacccgggag
      601 aggtggaagt agctgtaata gcgcctggcc gcgggatcgt agacgatgac gccgttgcct
      661 gagttggggg agaggcctcc cgagcggtag gccgaggggc cgccgccgcc cttccagtcg
      721 tcggccgcgg ccaggacgat gccggaggag acggagagta tctcggggcc gatctcgccc
      781 gaggggctac gctcgatcct ggtgaagaac acgtccaggg cgtagggatg ggccctgtcc
      841 aggccccgct tcgggggggc atagacctcg ccgcggcggg cgacggggcc agtggcttcc
      901 ggacctggtg aagcggactt ccgggttgcg agggcgtcat aggccgattc gaggagggcg
      961 ccgtagaggc cgcggatcga acgcatgagg ggcacgagcc gggcgatctc ggcgtaggct
     1021 tccgcgtgcc gcagcgtcgc cggggcgtcg cgggcggccg ccgcgaggaa gtccctgcgg
     1081 tcgcggtcgg tggcgaggag ggccgagacg gctcctccct tgggcaggac tgctcccagg
     1141 gcacgttccg gatccatgcc gcccgccttc gctgcctcga cgagggcccg cgccgccttg
     1201 cccgcttcct cggccgaaag gcctacgagg cgatcgggag cgaaggccgc ggagagttcg
     1261 gagaccgagg ctctcgcgga gacaagttcg gcgacgctcc gggcgtaggc gggcctgagg
     1321 ctctcgaagc ccgccgcgcc cgcgggcgac gcagcctctc ggggctcggc cgcggcatcg
     1381 ctcttgcccc gcatgacgct gaaggcagcc ctatccgagg cgttccccgc gaagccggcc
     1441 gccgccaggc ctccggctgc caggaggcag aggaaaacga tcggcgcggc cttgcgcggc
     1501 gcctctctcc gttccgccgc gccggaccgc ggctgggccg tgtcctgtac ggaacgggtc
     1561 cccgtggctc gcgtgtcggc gtcgaccgag gcccccgcag gggccgtcgc gacagactcc
     1621 tgtgtgggcg ccgcgggctt gtcggaatca agcgctccga tcccgggctc ggaggtccgg
     1681 agtgttgcga cctcgaccat ttccgcctct tgtacggcgg cttcgtgttc tgcggcctca
     1741 agctcggcgg cctcaagctc ggcagcctcg gcggcctcgg ggagtccgat caacgggtct
     1801 tccccccgtc cagccggagc gcggaggcca ggaagagctc ggcgcaggcg cgggcgtcgt
     1861 ccagggcgcg atgcgcgcgc gcatggccta tccccaggcg ggcggccagg tcgacgaggc
     1921 ggtagctcgg aaggccggga aaggcggcct tcgcgacgat gcgcgtgtcc aggacctcgt
     1981 tcgccgcggg ggggaggccc gccctggaaa gctcggcccg caggaaggag aggtcgaagg
     2041 gcgcgttgtg cgcgatgatg acagcgcccc gggcgagctc cgcgagggcg ggggccaggg
     2101 ccgcgaaggc cggctcgccc tcgacgtcct tgtcgctgat gccgtggatg gcggtggtca
     2161 cgggcgggat gggcttgccg ggattcacga ggagggagag gctgccctcc tcgccgagcg
     2221 tgagcctttc cgctccgggg ccgccgggcc ccgcgccgcg gaaagccccg ccgccgtcag
     2281 ccgcctcgcg gccggcctcg ccgccgtagt cggcctcgcc gccaagggcc gcggcgaaga
     2341 gagggcccag gtcggcctgg ccgcaaggct cgacgaacct gaagcgcagg gcgccgatct
     2401 cgacgacgcg ctcgcgggcg gggtccaggc ccgtcgtctc gaagtccagg gccaggtagg
     2461 tccgcgtccg ccagtcctcg gggaggacgc ggcgggccgc ggggggcgcg gcgccgtcgc
     2521 cctgggccgc gcggccgcca tcgcgggaat acgggacgtc ggcgccgccc gagggacggc
     2581 gccgcgcgta ggagtacccg cggttcggcc ggccgctcac cggtcgatga ccttcctcac
     2641 gtcggcctcg atggcggcgg ccttcttcgc cgaggccgcg cgggccgcgg ccagggccgc
     2701 ggggccggcg gccttcccgc cctcgacctc ggtcctgagg aggatgtagt acttgatctt
     2761 gggctcggtg ccgctcgggc gcacggtgag gacggtgccg tcctcgaggt accactggat
     2821 gacgtcgctc gcggggagct cgaccttctc ggcggcctcg ggcctgccgg cctgccactt
     2881 cctgcccgtc ttcacgtcgc ggatctcggc gaccttgatg ccgccgaagc ccgcgggctg
     2941 gtccttgcgg taggcctcca tgatgccgcg catgatgtcc atgccggcgg ggccctcgaa
     3001 gtacttgttg acgcccttct cctcgaagta cccgtgctcg aggtagagct cgtccaggcg
     3061 gtccaggagg ctcttgccct tggacctcca gtagagggtc atctccgcgg tcagggccgc
     3121 ggcggagatg ccgtccttgt cgcggacctc gggctcgacg aggtggccgt agctctcctc
     3181 ggtgccgtag aggaagtcga cgccctcggc ctcgagcttc cgcatgagct cggcgatcca
     3241 cttgaagccg gtgaggcact cgcggcactc ggcgccgtag gccttcgcta tcctgtcctg
     3301 caggctcgtc gtgacgatgc tcctgatggc ggcggggcgc cggggcatga ggccgagctc
     3361 cttccgcgtg aggaggatgt agtccaggtg gagggagccg agctggttgc ccgtgatgag
     3421 gacgaagtcc cccttggcgt ccgggacggc gatgcccagg cggtcggcgt ccgggtcggt
     3481 cgccatcacg cagtcggcct tcaccttcgc gccgagctcc agggcgagct tgagggcggc
     3541 ggtctcctcg gggttcggga aggcgaccgt ggggaaggcg ccgtccggct cccgctgctc
     3601 ggggacggtg acgacctcga ggccgagctc gcccatgatc cgctcgaagt ggagggcgcc
     3661 cgtgccgtgg aggggggtgt agacgatctt cacgctcttc ccgagctcct tgatgagggc
     3721 ggggcggagg agcttcgact tcaccatcgc gacgtaggga tcgtcgatct ccctgtcgat
     3781 gaccttgagg aggcccttct ccatcgcctc cgccttaggc atgagcttcg gggccttcac
     3841 ggcgttgacc atgtcgatga tgcccgtgtc gtgcggcggc gtgacctggg agccgtcgtt
     3901 ccagtaggcc ttgtagccgt tgtacttggg cggattgtgg ctcgcggtga cgacgacgcc
     3961 cgtgtcggcc ttgagctcgc ggatcgcgta ggagagctcg ggcgtaggcc ggagggccga
     4021 aaagaggtag gccttgacgc cgttggccgc gaagacgcgg gcggtcatct cggcgaagac
     4081 gt
//
LOCUS       DAOOSJ010000239         3991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_14386_length_3991_cov_7.452236, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000239 DAOOSJ010000000
VERSION     DAOOSJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14386_length_3991_cov_7.452236"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2954
                     /locus_tag="PLG14_10535"
     CDS             <1..2954
                     /locus_tag="PLG14_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOX49611.1"
                     /translation="HCSAFINSDIRKAVQQGATREDITAGIVYSIVANYLNRVVGNRS
                     IGKSVFLQGGVAKNEAVPLAFASLLGKPILVPPSPELMGCLGVGLLAKAKAAEGLLER
                     GSFDLSEMLDREITYESVFPCKSCENFCPIQVLSVSGRKYMFGGRCNKYANLRKSGAE
                     AAATDWVERRARLMFEECAPDPATFVRKRPEVVGIPRCFSVHSLYPFYARLFHELGLR
                     TFLSEDVAREGVARAESAYCFPAEIAHGAVQDAWDRGADYVFLPHFRDMPSYEKKVHA
                     NFCPITQALPYYIGKAFPDIPKERLLPVVVTFKFGRRKALEHFLAMGETLGIGAAEME
                     AAFDAACAAQDRYFERAAELGREALAEARASGRPAIALLGRPYNAFTQEANMGIPRKF
                     ATRGRSVVPFDILPFGDRTIFANMYWYYGQQDMKAAAFAKDEANLFVAYVSNFSCAPD
                     SFILHYLKWTMGQKPFLVLELDSHSADAGIDTRVEAFLDIVDGYRSKLGELKGERYDN
                     GLRFVNDGRSPLHVLDGRSGRRHELFGNPKVKLLLSNMGELSTELMAAVLRGSGVAAE
                     AMPVADERTIQVARAHASGKECVPSHLVLGSALQYFASDKYRKDEIYLLFVPITTGPC
                     RTGQYFVFYENLFRDLRLENVVVVTLSADNSYGEFGPRFSRDVWFSLAIGDYMKDLQN
                     SLRACAEDRKAARAWFDSSWRELIALAETDIRRVFPALRRIASGAAALPLAVRLEDAP
                     RVLVVGEIYVRRDDFAVDELVELFSARRIVAKVSGICEWIHYLDFVREYDLRKRLGLL
                     PRLSRPFSLESRKLLRLRAEEAWKHWVERRVRKALAPSGLIPRTPHDMRRIMANTSEH
                     FVSHELNSEIAVSSGVAATAMEDGYSGIVNISPFACLIGRVIEGLFAPWARERGYPMI
                     SVEVDGNLLPPSLVSKLDIFMVNVLRFRGRGDSGRLVEAARPAERAGAKPPEGAEHPA
                     GASAAKAREAE"
     gene            2951..3778
                     /locus_tag="PLG14_10540"
     CDS             2951..3778
                     /locus_tag="PLG14_10540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX49612.1"
                     /translation="MKDARNKQGPGTALVTGASGGIGAEFARALSREGWRLVLAGRNA
                     ERLESVRAGLQGPRAKEAIALQADLSEPGAGERLHRACREGGLEVDLLVNNAGSGLLG
                     QSADLPPAALEAMAALNVTALTVLSSLFGRDMRDRGSGRILNVGSFAGLNATPYFAAY
                     AATKSYVLSFSLALRAELKPRGVGVTCLLPGYVRTNFDAAAGIRSAKYLAFSERNAMD
                     AADVARIGLRALARGRAWTVAGARNRLAATVLGLLPRAAAPALMKASLDGLLAKEGR"
     gene            3775..>3991
                     /locus_tag="PLG14_10545"
     CDS             3775..>3991
                     /locus_tag="PLG14_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49613.1"
                     /translation="MKTNFSVVPKLMAKDPRLAAAIVRAFWAQKKSMLVDRTFRDGRT
                     SELGLMYFRLTPLCNLRCVMCGQRGDKG"
BASE COUNT          599 a         1479 c         1315 g          598 t
ORIGIN      
        1 agcactgctc ggccttcatc aactcggaca tccgcaaggc cgtccagcag ggcgcgacgc
       61 gggaggacat caccgcgggc atcgtctact cgatcgtcgc gaactacctg aaccgcgtcg
      121 tcgggaaccg cagcatcggc aagtccgtct tcctccaggg aggcgtcgcg aagaacgagg
      181 ccgtgcccct cgccttcgcc tccctcctcg ggaagcccat cctcgtcccg cccagccccg
      241 agctcatggg ctgcctgggc gtgggcctcc tcgcgaaggc gaaggcggcg gagggcctcc
      301 tcgagcgcgg ctccttcgac ctctcggaga tgctcgatag agagataacc tacgagagcg
      361 tctttccctg caagtcctgc gagaacttct gccccatcca ggtcctctcc gtctccggcc
      421 gcaagtacat gttcggcggc cgctgcaaca agtacgcgaa cctcaggaag agcggcgcgg
      481 aggccgcggc gaccgactgg gtcgagcggc gggcccgtct catgttcgag gaatgcgccc
      541 ccgaccccgc gaccttcgtc cggaagcggc ccgaggtggt cggcatcccc cgctgcttct
      601 cggtccacag cctctacccc ttctacgcgc ggctcttcca cgagctcggc ctcaggacct
      661 tcctctccga ggacgtggcg cgcgagggcg tcgcgagggc ggagagcgcc tactgcttcc
      721 ccgcggagat agcccacggc gccgtccagg acgcctggga ccggggcgcc gactacgtct
      781 tcctgcccca cttccgggac atgccgagct acgagaagaa ggtccacgcg aatttctgcc
      841 ccatcaccca ggccctgccc tactacatcg gcaaggcctt ccccgacatc ccgaaggagc
      901 gcctcctccc cgtcgtcgtc accttcaagt tcgggaggag gaaggccctg gagcacttcc
      961 tcgcgatggg cgaaaccctg ggcatcggag ccgcggagat ggaggcggcc ttcgacgcgg
     1021 cctgcgcggc ccaggaccgc tacttcgagc gggccgcgga gctcggccgc gaggccctgg
     1081 cggaggcccg cgcctccggc cggccggcga tcgccctcct ggggcggccc tacaacgcct
     1141 tcacccagga ggcgaacatg ggcatcccgc ggaagttcgc gacgcggggc cgctcggtcg
     1201 tccccttcga catcctcccc ttcggcgacc ggaccatctt cgccaacatg tactggtact
     1261 acggccagca ggacatgaag gccgccgcct tcgcgaagga cgaggcgaac ctcttcgtcg
     1321 cctacgtctc caacttctcc tgcgcccccg actccttcat cctccactac ctcaagtgga
     1381 cgatgggcca gaagcccttc ctggtcctcg agctcgactc gcactcggcg gacgcgggca
     1441 tagacaccag ggtggaggcc ttcctggaca tcgtggacgg ctaccgctcc aagctcgggg
     1501 agctcaaggg cgagcgctac gacaacgggc tccgcttcgt gaacgacggc cgttcgcccc
     1561 tccacgtcct ggacgggcgc tcgggccgga ggcacgagct cttcgggaac cccaaggtga
     1621 agctcctcct ctccaacatg ggcgagctct ccaccgagct catggccgcc gtgctccggg
     1681 gctccggcgt agccgccgag gccatgcccg tcgcggacga gcgcaccatc caggtcgcgc
     1741 gcgcccacgc ctccgggaag gagtgcgtcc cctcccacct cgtcctgggc tcggccctgc
     1801 agtacttcgc ctcggacaag taccggaagg acgagatcta cctcctcttc gtgcccatca
     1861 cgacggggcc ctgccgcacc ggccagtact tcgtcttcta cgagaacctg ttccgggacc
     1921 tgcggctcga gaacgtggtc gtcgtgaccc tgagcgcgga caactcctac ggcgagtttg
     1981 gcccccgctt ctcccgcgac gtctggttca gcctcgcgat aggcgactac atgaaggacc
     2041 tgcagaactc cctgcgggcc tgcgcggagg acaggaaggc cgcacgggcc tggttcgact
     2101 cctcctggcg ggagctcatc gccctggcgg agaccgacat ccgcagggtc ttccccgccc
     2161 tcaggcggat agcctcggga gcggcggccc tgcccctcgc ggtccggctc gaggacgcgc
     2221 cgcgcgtcct cgtcgtgggc gagatctacg tgcggcgcga cgacttcgcc gtggacgagc
     2281 tcgtggagct cttcagcgcg cggcgcatcg tcgccaaggt ctccgggatc tgcgagtgga
     2341 tccactacct ggacttcgtg cgggagtacg acctccggaa gcggctcggc ctcctgccgc
     2401 gcctctcgcg gcccttctcc ctcgagtcgc ggaagctcct ccgcctccgg gccgaggagg
     2461 cctggaagca ttgggtcgag cggcgcgtgc gcaaggccct cgcgccttcc ggcctgatcc
     2521 cgaggacgcc gcacgacatg cgcaggatca tggccaacac gagcgagcac ttcgtgagcc
     2581 acgagctcaa ctccgagatc gccgtctcct ccggcgtcgc cgcgacggcg atggaggacg
     2641 gctactccgg catcgtcaac atctccccct tcgcctgcct gatcgggcgg gtgatcgagg
     2701 ggcttttcgc cccctgggcc agggagcggg gctacccgat gatctccgtg gaggtggacg
     2761 gcaacctcct cccgcccagc ctggtgagca agctcgacat cttcatggtc aacgtcctgc
     2821 gcttccgcgg ccgcggcgac tcgggccgcc tcgtggaggc ggcccggccc gcggagcggg
     2881 cgggcgcgaa gccgcccgag ggggcggagc atccggcggg cgcgtcggcg gccaaggcaa
     2941 gggaggcgga atgaaggacg caaggaacaa gcagggtccg ggcacggccc tagtgacggg
     3001 cgcctcgggc ggcatcggcg cggagttcgc ccgcgccctc tcgcgggaag gctggaggct
     3061 cgtcctcgcg ggccggaacg cggaaaggct cgagtcggtg cgggcgggcc tccaaggccc
     3121 gcgcgcgaag gaggcgatcg ccctccaggc cgacctctcc gagccgggcg ccggggagcg
     3181 gctccaccgg gcctgccgcg aagggggcct cgaggtggac ctcctcgtca acaacgcggg
     3241 ctcgggcctc ctgggccaga gcgcggacct cccgcccgcc gcgctggagg cgatggcggc
     3301 gctcaacgtc acggccctca ccgtcctgtc ctccctcttc ggccgcgaca tgcgggacag
     3361 gggaagcggg aggatactca acgtcggctc cttcgcgggg ctcaacgcca cgccctactt
     3421 cgcggcctac gcggcgacga agagctacgt cctctccttc tcgctcgcgc tcagggccga
     3481 gctcaagccc aggggggtcg gcgtgacctg cctcctcccc ggctacgtgc gcacgaactt
     3541 cgacgcggcg gccggcatcc gcagcgccaa gtacctggcc ttctcggagc gcaacgccat
     3601 ggacgccgcc gacgtggcga ggatcggcct ccgcgccctg gcgcggggaa gggcctggac
     3661 ggtcgcgggt gcgcggaaca ggctcgctgc caccgtcctc ggcctccttc cccgcgccgc
     3721 ggcgcccgcc ctcatgaagg cctcgctcga cggactgctc gccaaggagg gacgatgaag
     3781 accaatttct cggtggtgcc caagctcatg gccaaggacc cccgcctggc cgcggccatc
     3841 gtccgcgcct tctgggccca gaagaagtcc atgctcgtcg accggacttt ccgcgacggg
     3901 aggacgagcg agctcggcct catgtacttc cggctcacgc cgctgtgcaa cctgcgctgc
     3961 gtcatgtgcg gccagcgcgg cgacaagggc g
//
LOCUS       DAOOSJ010000240         3951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_14614_length_3951_cov_7.358060, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000240 DAOOSJ010000000
VERSION     DAOOSJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14614_length_3951_cov_7.358060"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLG14_10550"
     CDS             <1..666
                     /locus_tag="PLG14_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49614.1"
                     /translation="KTAYEMTYPGSQVLDQKDGTWILAFEMDGARIPKDPGYVRTVKV
                     GPANPNIDGHNSVRMVRRLVVKQKDFKPFSLKLEGKMGFELDRATVQSCVSCHKRTVT
                     YEAKGKTAVYTGFPAYLALGYVDDPKYAPHKQDKSLAAYDEAAAKAGYKVVFAAADGF
                     KVTLDSRELNRNEDVIIAMYKDEAILPEGEFPLALAWDRGAKLVPAGIKGLRQLASIR
                     AEF"
     gene            666..2495
                     /locus_tag="PLG14_10555"
     CDS             666..2495
                     /locus_tag="PLG14_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX49615.1"
                     /translation="MPKRRRGPPWGFLPLGALLLVAGLLPFAVALVDSFFHDGYGGRS
                     FAGLDNYRRLAGDRAFGLSLLITLAWASLSALLSTSIGFALATLLHESKRIFRLLYLA
                     LLVPWGVPAFIAVPVWRMLIHGAGGDSVLSSLFGIRVNLLTDPLPSFAAALLVAAWMN
                     APAAVFVLYGSLRRVPRRVVEAAALDGAGPAALARRIYLPQVSASLVAVAALEFAKAF
                     KEFNVPFLLTAGGPSTLGGITERSIVGATTTVEVYLYELFQSSEDFGLAAAYAAVLAA
                     AACLLVGLAQLARKAAEARGAALREAGPAAAGGDPGGSEAAPELPRRVRRRALGLPGP
                     AADAAWTGARLALAAAAAASSLLAAYAVLWASFSGLSSNYIDGILPRFPSARSFVAIF
                     AEDGIWRPFLNTLGVSGAAALLMPALALPAALRLRSFRAGTKAWAFAIVQALGSAGGM
                     HSLVPLYVLFRSLGMTDSYVPVVIVYLFHAAPFALFTTAAFLAGLPPGLEEAAELEGA
                     GPWARLRLVILPLAWPAVATCAMTAFLAAWNGFLAPLLFLSDDAKYTIGLRLHAYVGS
                     VASGAPRWNRFAAASIINLALVGLVFYRFRAPLSNADAAEREE"
     gene            2766..3713
                     /locus_tag="PLG14_10560"
     CDS             2766..3713
                     /locus_tag="PLG14_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49616.1"
                     /translation="MAGRSAAQLVDIRLVEDKRIDDSYFQDYAKIHERLERVLEKEPK
                     PMRDPQANAQADAFVAWLAGQSAKGLSNERLSMLLRCGLGHICAEMIAAPPSLSLDEI
                     AARAYAELQRRGWDLAVYDPEVAADAVKRVEEAAKAAGSRARAGTKKDPSSAPAGAGV
                     ISKVGKGTPKVAVAKASTKAAPAKKEAHMIEKKKSPAKPAAAKKAPAKKAAAKKPAAK
                     KPAKKVAEKKAPAKKAAAKKPAAKKAVAKKPVAKKAVAKKPVAKKAAPAKKLAEKKPV
                     AKKAVAKKPVAKKAAPAKKPVAKKAAAPKKAPAKKPAAK"
BASE COUNT          589 a         1537 c         1266 g          559 t
ORIGIN      
        1 aagacggcat acgagatgac ctaccccggc tcccaggtcc tcgaccagaa ggacgggacc
       61 tggatcctcg ccttcgagat ggacggcgcg cgcataccca aggatcccgg ctatgtgcgc
      121 acggtcaagg tcgggcccgc caaccccaac atcgacggcc acaattccgt ccgcatggtc
      181 aggcgcctcg tggtcaagca gaaggacttc aagcccttct ccctcaagct cgagggcaag
      241 atgggcttcg agctcgaccg cgccaccgtg cagagctgcg tcagctgcca caagcggacg
      301 gtcacctacg aggccaaggg caagaccgcg gtctacaccg gcttcccggc ctacctcgcc
      361 ctgggctacg tcgacgaccc gaagtacgcg ccccacaagc aggacaagtc cctcgcggcc
      421 tacgacgagg cggcggccaa ggccggctac aaggtcgtct tcgccgccgc cgacggcttc
      481 aaggtgaccc tggattcccg cgagctcaac cgcaacgagg acgtgatcat cgccatgtac
      541 aaggacgagg ccatcctccc cgagggcgag ttccccctgg ccctggcctg ggaccggggc
      601 gccaagctcg tgccggccgg catcaagggc ctccgccagc tcgcctcgat ccgggccgag
      661 ttctgatgcc caagaggagg cgcgggccgc cctggggctt ccttcccctc ggcgccctcc
      721 tcctcgtcgc ggggctcctg cccttcgccg tcgccctcgt cgactccttc ttccacgacg
      781 gctacggcgg gcggagcttc gcgggcctgg acaactaccg ccgcctcgcc ggggaccggg
      841 ccttcggcct ctccctcctc atcaccctgg cctgggcctc cctctcggcc ctcctctcga
      901 cctccatcgg cttcgccctc gccaccctcc tccacgagtc gaagcggata ttccgcctcc
      961 tctacctcgc cctcctcgtg ccctggggcg tgccggcctt catcgcggtg ccggtctggc
     1021 gcatgctgat ccacggggcg ggaggggatt cggtcctctc ctccctcttc ggcatccgtg
     1081 tgaacctcct cactgacccc ctgccatcct tcgccgcggc cctcctcgtc gccgcctgga
     1141 tgaacgcgcc ggccgcggtc ttcgtgctct acggctccct gcgccgggtg ccgcggcggg
     1201 tcgtggaggc ggccgccctc gacggagccg ggcctgcggc cctcgcccgg cgcatctacc
     1261 tgccccaggt ctcggcctcc ctcgtcgccg tcgcggccct cgagttcgcg aaggccttca
     1321 aggagttcaa cgtgcccttc ctcctcacgg cgggggggcc ctcgacgctc ggcggcatca
     1381 ccgagcggag catcgtcggg gccacgacga cggtcgaggt ctacctctac gagctcttcc
     1441 agagctccga ggacttcggc ctggcggccg cctacgccgc cgtcctggcc gcggcggctt
     1501 gcctcctcgt gggcctggcc cagctcgcgc ggaaggccgc cgaggcgcgc ggagcggccc
     1561 tccgcgaggc cgggccagcc gccgcgggcg gcgacccggg cggcagcgag gccgcgccgg
     1621 agctcccgcg ccgcgtgcgt cgccgcgcgc tcggccttcc cgggccggcc gccgacgcgg
     1681 cctggacggg agcccgcctc gccctggccg cggcggcggc ggccagctcc ctcctcgccg
     1741 cctacgccgt gctctgggcc tccttctcgg gactctcctc gaactacatc gacggcatcc
     1801 tgccccgctt cccgagcgcc cggagcttcg tcgcgatctt cgccgaggac gggatctggc
     1861 ggcccttcct caacaccctc ggggtctccg gggccgcggc cctcctcatg cccgccctcg
     1921 ccctgcccgc ggccctgcgc ctgcggagct tccgggcggg gaccaaggcc tgggccttcg
     1981 ccatcgtcca ggccctcggc tccgccggcg gcatgcactc cctcgtgccc ctctacgtcc
     2041 tcttccgctc cctgggcatg accgattcct acgtccccgt cgtgatcgtc tacctcttcc
     2101 acgccgcccc cttcgccctg ttcaccacgg cggccttcct cgcgggcctg ccgccggggc
     2161 tcgaggaggc cgccgagctc gagggcgcgg ggccctgggc gcggctccgc ctcgtgatcc
     2221 tccccctggc ctggcccgcg gtcgccacct gcgcgatgac ggccttcctc gcggcctgga
     2281 acggcttcct cgcgcccctg ctcttcctca gcgacgacgc gaaatacacg atcggcctgc
     2341 ggctccacgc ctacgtgggc tcggtggcct cgggcgcgcc ccgctggaac cgcttcgccg
     2401 cggcctccat catcaacctc gccctggtcg ggctggtctt ctaccgcttc cgcgccccgc
     2461 tctcgaacgc ggacgcggcc gagcgcgagg aatgatccgc gggccgcgcc gagcgcccag
     2521 gcggaacgcg gggaagctcg cgcgcgcggt cccgcgcgcg ccgcgcgggg ctcggaacgg
     2581 cgcgcgcgaa gggaattttg ccttcgcgaa gcgagcgcgc tattgttctc gcgaagaatg
     2641 ccgataaaat aatgaggctc gaagacgttt tttcgcgaga tcgagcttca tccacttgcg
     2701 ttattggatg aagtgagtaa ttgtataggg caatagatcg agcataccaa agggggacgc
     2761 cctagatggc tggaagaagc gccgcgcaac tcgtcgacat caggctcgtc gaggacaagc
     2821 gcatcgacga ttcctacttc caggactacg ccaagataca cgagcggctc gagcgggtcc
     2881 tcgagaagga gcccaagccc atgcgggacc cccaggccaa cgcccaggcc gacgccttcg
     2941 tcgcctggct cgcgggccag tccgcgaagg gcctgtccaa cgagcggctc tccatgctcc
     3001 tgcgctgcgg cctcggccat atctgcgccg agatgatcgc cgcccccccc tccctctccc
     3061 tcgacgagat cgccgcccgc gcctacgccg agctccagcg gaggggctgg gacctcgccg
     3121 tctacgaccc cgaggtggcg gcggacgccg tcaagcgcgt cgaggaggcg gccaaggccg
     3181 ccggatcccg cgcccgcgcg ggaacgaaga aggacccctc gtcggcgccc gcgggcgccg
     3241 gcgtaatctc gaaggttggg aaaggtacac ccaaggtcgc cgtagccaag gcttccacga
     3301 aggccgcgcc ggcgaagaag gaggctcaca tgatcgagaa gaagaagtcg ccggcgaagc
     3361 cggcggcggc caagaaggcc ccggccaaga aagcggcggc taagaagccc gccgccaaga
     3421 agcccgccaa gaaggtcgcc gagaagaagg ccccggccaa gaaggccgcc gccaagaagc
     3481 cggccgccaa gaaggccgtc gcgaagaagc cggtcgccaa gaaggccgtc gcgaagaagc
     3541 cggtcgccaa gaaggccgct cccgcgaaga agctcgccga gaagaagccc gtcgccaaga
     3601 aggccgtcgc gaagaagccg gtcgccaaga aggccgcccc cgcgaagaag ccggtcgcca
     3661 agaaggccgc cgcccccaag aaggcgcccg ccaagaagcc cgcggccaag taagcgccgc
     3721 gcgccagacg tagaaaaagg gggactgccc acagcgggcg gtcccccttc attatatctc
     3781 cgtgcgtccg tcgaacgcgg ctcccgcgtg gccgcgtctg gcgccgcgcc tcgatgtgcc
     3841 gcggccaccg aatgccgcgc cgcgaggtgc cgcggccgcc gcccgcggga ctcccctcta
     3901 cgcccgccgc ttcgtgaagc ccatgatgag gcccagggcg acggaccagc a
//
LOCUS       DAOOSJ010000241         3899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_14885_length_3899_cov_6.188345, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000241 DAOOSJ010000000
VERSION     DAOOSJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14885_length_3899_cov_6.188345"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLG14_10565"
     CDS             complement(<1..360)
                     /locus_tag="PLG14_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOX49617.1"
                     /translation="MARILAKRRLSEEVYRMEIEAPNVARERRAGQFVIVQADADFGE
                     RIPLTIADADPSRGSVSLIFQTVGASTHALAEKEPGDEIVLLGPLGNPTHIEKVGWAV
                     CVGGGIGLAPLYPIVKAL"
     gene            complement(360..2255)
                     /locus_tag="PLG14_10570"
     CDS             complement(360..2255)
                     /locus_tag="PLG14_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOX49618.1"
                     /translation="MKGFRARAVGSMLIVTLAVIVGLQVYDAVFINRDPGALLANLPR
                     VLAVSSLLLALASILLLRILKPLADTAAAAGRGERPSEEARAAAFDVQPKANRLVVGV
                     LVAAFAVGPVLILLAAAAKGSSFSAVLVAKILATNLACGFMAAIQIVVLMEEALRPSI
                     VRLDLVRVPASSRKASLRGWVLLAAAAGSLVMAVLLGVGGLSAIESAAAVALTPLAFI
                     LKAGLLALALAAWSASLFLSIGGVVGRQALVLEDRLGEILRGGGDLTMRVPVIRNDEI
                     GRLSGRLNHFLDVVEATLLKVRELSGKVQSGANSLAESAEHAGGSVGALESSVATMRD
                     AAERQAESLRAAEGEIERMVVSIDRVADRVADQASAVEQSSAAVSEMVANIASVSRVS
                     AQADGIAGALRTASADGGEALKASLAAIREIEEASRSVKEIIGVISKIAAQTNLLAMN
                     AAIEAAHAGEAGRGFAVVADEVRGLAESASRSAKEIVGLIQGMNQKIERGAALADRAG
                     QAFGRITEGVEETGELVRTIASSMSEQQGGAEEILKSANALTDATQGIKALTMEQKEE
                     SKEMAAAMQRVVAASGDIVEAAQEGAGSTQSLGRVVGLVGEEAARNRALVAGLEEAVG
                     KFRFGSR"
     gene            2559..3332
                     /locus_tag="PLG14_10575"
     CDS             2559..3332
                     /locus_tag="PLG14_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOX49619.1"
                     /translation="MPVPLDPDKYRSAPFFNPEDFIGYIRRLGRLGARPAPEAVILSY
                     QRSLFDYVEAEVPSSPAEGYFAPQMRYLEGTGGRVAVVGRFGVGAPAAAVMLEELIAF
                     GVRRFVSVGTAGSLVASVKPGDLVLCDGALRDEGTSYHYVPEGGSALPSPGLTERLGA
                     ALSRKGLAHARGPAWTLDAIYRETPAEVKRFREEGALVAEMEAAALFTVAAFRGAQIA
                     ACFSVSDTLAELAWRPEFHAETTEGGLRLIFEAALEALA"
     gene            3579..>3899
                     /locus_tag="PLG14_10580"
     CDS             3579..>3899
                     /locus_tag="PLG14_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49620.1"
                     /translation="MPDTKNMVMIDGNTAAAHVAHAVSEVIAIYPITPSSPMGELSDE
                     YSAQGRKNIWGTVPSVVEMQSEAGAAGAVHGALTTGALTTTFTASQGLLLMIPNMYKI
                     AGECT"
BASE COUNT          560 a         1397 c         1380 g          562 t
ORIGIN      
        1 gagcgccttc acgatggggt agaggggggc gaggccgatg ccgccgccca cgcagaccgc
       61 ccagcccacc ttctcgatgt gcgtcgggtt ccccaggggg ccgaggagga cgatctcgtc
      121 gcccggctcc ttctccgcca gggcgtgggt gctcgccccg accgtctgga agatgaggct
      181 cacggagccg cgcgagggat ccgcgtcggc tatcgtgagg gggatgcgct cgccgaagtc
      241 cgcgtccgcc tggacgatga cgaactgccc cgccctgcgc tcgcgcgcga cgttgggcgc
      301 ctcgatctcc atgcggtaga cctcttccga aaggcggcgc ttcgccagta ttctcgccat
      361 cacctgctcc cgaacctgaa tttcccgacg gcttcctcga ggcccgcgac gagggcgcgg
      421 ttcctggccg cctcctcgcc cacgaggccc acgacgcggc ccagggattg ggtcgagccc
      481 gcgccttcct gcgcggcctc cacgatgtcc ccggaggcgg cgaccacgcg ctgcatggcg
      541 gcggccatct ccttggactc ctctttctgc tccatggtca gggccttgat gccctgcgtc
      601 gcgtcggtga gggcgttggc gctcttgagg atctcctccg ccccgccctg ctgctcgctc
      661 atggaggagg cgatggtcct gacgagctcg cccgtctcct ccacgccctc cgtgatgcgg
      721 ccgaaggcct ggcccgcgcg gtcggccagg gcggcccccc gctcgatctt ctggttcatg
      781 ccctggatga ggccgacgat ctccttggcg ctccgggagg cggactccgc gaggccgcgc
      841 acctcgtcgg cgacgaccgc gaagccccgg cccgcctcgc ccgcgtgggc cgcctcgatg
      901 gcggcgttca tcgcgaggag attcgtctgg gcggcgatct tggagatgac cccgatgatc
      961 tccttcacgg agcgggaggc ctcctcgatc tcgcggatcg cggccaggga ggccttgagg
     1021 gcctctcctc cgtcggcgct cgccgtcctg agggcgccgg ctatcccgtc cgcctgggcg
     1081 gagacgcgcg acacggaggc gatgttggcg accatctcgc tcaccgccgc cgagctctgc
     1141 tccacggcgc tcgcctggtc ggcgacgcgg tccgcgacgc ggtcgatgga gaccaccatg
     1201 cgctcgatct cgccctccgc ggcgcgcagg gactcggcct ggcgctctgc ggcgtcgcgc
     1261 atcgtcgcca cggaggattc cagcgccccg accgagccgc cggcgtgctc tgcggactcc
     1321 gcgagggagt tggcgcccga ctggaccttc ccggagagct cccgcacctt gaggagggtc
     1381 gcctccacga cgtccaggaa gtggttgagc ctccctgaga ggcggccgat ctcgtcgttc
     1441 ctgatgacgg ggacgcgcat cgtgaggtcg cccccgcctc ggaggatctc gcccagcctg
     1501 tcctccagga ccagggcctg ccggccgacg acgccgccga tggaaaggaa gagggaggcg
     1561 gaccaggcgg cgagggccag ggcgaggagg ccggccttga ggatgaaggc caggggcgtg
     1621 agcgctaccg cggcggccga ctcgatggcg gagaggccgc cgacgcccag gaggacggcc
     1681 atgaccaggg agccggccgc ggcggccagg aggacccagc cgcggaggga ggccttgcgg
     1741 ctggaggcag ggacgcggac caggtccagc cggacgatgg agggccgcag ggcctcctcc
     1801 atgaggacga cgatctggat ggccgccatg aagccgcagg cgaggttcgt ggccaggatc
     1861 ttcgcgacga ggacggcgga gaaggagctt cccttcgcgg ccgcggccag gaggatcagg
     1921 acggggccga cggcgaaggc cgcgacaagg acgccgacga cgaggcggtt cgccttgggt
     1981 tggacgtcga aggcggcggc ccgggcctcc tcggagggcc tctcgccgcg cccggccgcg
     2041 gccgcggtgt cggcgagcgg cttgaggatg cggagaagga ggatcgatgc gagggcgagg
     2101 aggagggagg agacggcgag gacgcggggc aggttggcca ggagggcgcc ggggtcgcgg
     2161 ttgatgaaga cggcgtcgta gacctgaagc ccgacgatga ccgccagggt cacgatgagc
     2221 atggagccta cggccctggc gcggaaaccc ttcatggtgc tgtacctccg ggatgccggt
     2281 cggcttggcg cccgagggcg gccggagcgg cggttctcca taatagacag gaggccaagt
     2341 tttgtaaaac ccgtatcgcg agaaatcggc gtcccgggag ggaggacggc gggctggaac
     2401 aggagtgccc ggccgtcgcc gccttgccgg gcgggctggc cgcggccgtg gccttggggc
     2461 tacactgtcc cggcgccggc cttccgccga tccgcggcgc gacgcgcgcc agcccccgga
     2521 gccgccgcga gagcatataa aggcgctgga ggaaagccat gcccgtcccc ctggatcccg
     2581 acaagtaccg ctccgccccc ttcttcaatc ccgaggattt catcggctac atccgccgcc
     2641 tgggtcggct cggcgcccgc ccggccccgg aggccgtcat cctctcctac cagcgcagcc
     2701 tcttcgacta cgtcgaggcc gaggtgccct cgagccccgc ggagggctat ttcgccccgc
     2761 agatgcgcta tctcgagggg acgggcggcc gggtagccgt cgtgggccgc ttcggcgtcg
     2821 gggccccggc ggcggccgtc atgctcgaag agctcatagc cttcggcgtc cgccgcttcg
     2881 tctccgtcgg gacggccgga agcctcgtcg cgagcgtgaa gccgggcgac ctcgtcctct
     2941 gcgacggggc cctgcgcgac gagggcacct cctaccacta cgtcccggaa ggcggctcag
     3001 ccctcccctc ccccggcctc accgagcgcc tgggcgccgc cctctcgcgg aaggggctcg
     3061 cgcacgcccg gggtccggcc tggaccctgg acgcgatcta ccgggaaact ccggcagagg
     3121 tgaagcgttt ccgcgaggaa ggcgcccttg tcgcggagat ggaggcggcg gccctcttca
     3181 ccgtcgcggc cttccgcggc gcacagatag ccgcctgctt cagcgtgagc gacaccctgg
     3241 cggagcttgc ctggaggccg gaattccacg cggagaccac ggaggggggc ctcaggctca
     3301 tcttcgaggc cgccctggag gccctcgcct aggaccggag ggactgaccg gccggccccc
     3361 ttagaggggc cccggccggc ccccgatcgg gcccgcgccg gccgccagga aggccccgga
     3421 gcgcccgttg ccccggaacg gccggccccc tcggccttgt cacgcctttt ttagcgactc
     3481 cccgtccggg ttattgatca aaacatcgaa tggttctact attgaaaccg gctcggaccg
     3541 agcctcccat ccccatccta tcacttcaag gaatccgtat gcccgatacg aagaacatgg
     3601 tgatgatcga cggcaacacc gccgcggctc acgtcgccca cgcggtgagc gaggtcatcg
     3661 cgatctaccc gatcaccccc tcttcgccca tgggcgagct ctccgacgag tactccgccc
     3721 agggccgcaa gaacatctgg ggcaccgtcc cctccgtcgt cgagatgcag tccgaggccg
     3781 gcgccgcggg cgccgtgcac ggagccctga ccacgggcgc cctcacgacg accttcacgg
     3841 cctcccaggg cctcctcctc atgatcccga acatgtacaa gatcgccggc gagtgcacc
//
LOCUS       DAOOSJ010000242         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_14986_length_3879_cov_4.104603, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000242 DAOOSJ010000000
VERSION     DAOOSJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14986_length_3879_cov_4.104603"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLG14_10585"
     CDS             <1..409
                     /locus_tag="PLG14_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOX49621.1"
                     /translation="MSDGRYGLVADEGAASGRGRIGLELLVRDAHTGRTRPAGTLSGG
                     ERFLAAVSLALGLADTIRSRSGGVALDAVFIDEGFGSLDEEALDRAVTALDRARGART
                     IGIVSHVPELKSRIASRIEVTKGRGGSTVELVE"
     gene            597..3641
                     /locus_tag="PLG14_10590"
     CDS             597..3641
                     /locus_tag="PLG14_10590"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOX49622.1"
                     /translation="MGNAAFEVYAIGVFIASLGSILLGAFSWFGAKREYARPFSLMQF
                     AIFVWCFFGYSQLRVSGLGEQVAYLKLEYIGIASLPVAALAFARSLARRPLSGRTALL
                     LLALPALTVLLVAVGGEPGLVWTPVGQDAKGRLLTEAGPWFWVHSAYSYVLVTACFAN
                     LVARAREVQGSTRRWLRHSIAILLIPVFTNLVFVLFFLKTTPLDPTPMAFALSGLLLA
                     IGLRNYNIFDLVPYAKEAILAAIDNPILTVDADRLVVGANAPALALFAGLGAIEGRPL
                     SALAGEDAGSALLAAAEASLREGGAREWRLRDRYYHLVSFALPTALGTGRDFILIFYD
                     ETERTRALLALEERERALERYASMVNASHEFMSIVGRDLRYEAVNDAFCERYGKCRDD
                     ILGATVFEVWGEEAARKYILPYLESCFAGEYKVIRNRFAFVGEEKERHFEVSYNPVRD
                     ASGEVTHAVVITKDVGDYVEAQEALAVAREKADAANAAKSSFLAAMSHEIRTPLNAII
                     GLTELSLRAPLSAEQRDNLETVRMAGGTLLNIINDILDLSKIEAGRMTLERVDFDLPA
                     HLAQVLKTFKPAFARKGLYLEFEAAPGSPRFVAGDSLRLGQVLMNLVGNAAKFTEKGG
                     VRVRLEAEAAGEGKAGIRVTVADTGIGIPPDKLDLIFESFSQADSSVSRRYGGTGLGL
                     SICRNLVGLFGGRIWVESEPGKGSTFSFTAFLEPGDEAKAAARIRPGEGEGEAAGGGP
                     FDILLAEDNLINAKVALRWLEQAGHRARHAVSGAEAIRLLSERPFDLVLMDVEMPDID
                     GLEAARRVRGGAAGLDRRAVPIIAMTAHDDQAMREACARAGMDDYVTKPVDFVELSGA
                     LARVARPSTREAAAKAPALVPASGEPLAAAPLLDEAEPLRRLGGDGDLYLELISIFAA
                     EARGRREALEAALAAREPETLRRLAHSLRGGARTIGAEPLAAAAAALEKAAGEADSSE
                     AGAASAMPGLERLQAAALVLLDSTARRIGQRLPPGYEAPQPE"
     gene            3698..>3879
                     /locus_tag="PLG14_10595"
     CDS             3698..>3879
                     /locus_tag="PLG14_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49623.1"
                     /translation="MARSSRLFPGIPSRGDSAPAGRTPAAGRPAGSRWRPWAEGAVAL
                     LFLLVFAVFAQSLLDA"
BASE COUNT          516 a         1465 c         1346 g          552 t
ORIGIN      
        1 gatgtccgac ggccgctacg gcctggtcgc cgacgagggc gccgcctccg gccggggccg
       61 gatcggcctg gagctcctgg tccgcgacgc ccacacgggg aggacccggc cggcgggcac
      121 cctctccggc ggcgagcgct tcctggccgc cgtgagcctg gccctgggcc tggccgacac
      181 tatccggagc cgctcgggcg gggtcgcgct ggacgcggtc ttcatcgacg agggcttcgg
      241 cagcctggac gaggaggccc tggaccgcgc ggtcaccgcc ctggaccggg cgcgcggcgc
      301 gcggacgatc ggcatagtct cccacgtgcc ggagctcaag agccgcatcg cctccaggat
      361 cgaggtgacg aagggacgcg gcggctcgac ggtcgagctc gtcgaatgag gcggccgcgg
      421 ggcgacacgg gtccgcgccg ccgcggcgtg gcccgggcgg ccgccgcgga gcggccgcag
      481 gcgcctgccc gcgggggctg gcgccccgcc tcccatggac tatagttatg gaatgggcgg
      541 attcgggccc cgccgccgga tcgtcgggaa aatcctttca agccggagcc tcgcaggtgg
      601 gtaacgcggc cttcgaagtc tacgcgatag gcgtcttcat cgcctccctc ggctccatcc
      661 tcctcggcgc cttctcctgg ttcggggcga agcgggagta cgcgcgcccc ttctccctca
      721 tgcagttcgc catcttcgtc tggtgcttct tcggctactc ccagctccgc gtcagcggcc
      781 tgggagaaca ggtggcctac ctcaagctcg agtacatcgg catcgcctcc ctgcccgtcg
      841 cggccctcgc cttcgcgcgg agcctggcgc gccgccccct ctcggggcgg acggccctcc
      901 tcctcctcgc cctcccggcc ctcaccgtcc tcctggtcgc ggtgggcggc gaaccgggcc
      961 tggtctggac gcccgtggga caggacgcga agggcaggct cctcacggag gccggcccct
     1021 ggttctgggt ccacagcgcc tactcctacg tccttgtcac ggcctgcttc gccaatctcg
     1081 tcgcgagggc cagggaagtc cagggttcga cccggcgctg gctccgccac tccatcgcga
     1141 tcctcctcat ccccgtcttc acgaacctcg tcttcgtcct cttcttcctc aagacgacgc
     1201 ccctcgaccc gacgcccatg gccttcgccc tctcgggcct tctcctcgcg atcggcctca
     1261 ggaactacaa catcttcgac ctcgtgccct acgcgaagga agccatcctc gcggcgatcg
     1321 acaaccccat cctcaccgtg gacgcggacc gcctggtcgt cggcgccaac gcgccggccc
     1381 tggccctctt cgcggggctc ggcgccatcg agggccgccc gctctccgcg ctggccggcg
     1441 aggatgccgg ctccgccctc ctcgccgccg cggaggcctc cctgcgcgag ggaggggcca
     1501 gggagtggcg cctgcgcgac cgctactacc acctcgtctc cttcgcgctt ccgacggccc
     1561 tggggacggg ccgggacttc atcctcatct tctacgacga gaccgagagg acccgcgccc
     1621 tcctcgccct ggaggagcgg gagcgggccc tggagcgcta cgcctccatg gtcaacgcct
     1681 cccacgagtt catgagcatc gtgggccggg acctccgcta cgaggcggtc aacgacgcct
     1741 tctgcgagcg ctacgggaag tgccgggacg acatactggg cgcgaccgtc ttcgaggtct
     1801 ggggcgagga ggccgcgagg aagtacatcc tcccctacct cgagtcctgc ttcgcgggcg
     1861 agtacaaggt catccgcaac cgcttcgcct tcgtcggcga ggagaaggag cgccacttcg
     1921 aggtgtccta caacccggtg agggacgcct ccggcgaggt cacccacgcg gtcgtcatca
     1981 cgaaggacgt gggcgactac gtggaggcgc aggaagccct ggccgtggcc cgcgagaagg
     2041 cggacgcggc caacgcggcc aagagctcct ttctggccgc gatgagccac gagatccgca
     2101 cgccgctcaa cgcgatcatc ggcctcacgg agctcagcct ccgcgccccc ctctccgctg
     2161 agcagcggga caacctggag acggtgcgta tggcgggcgg caccctcctc aacatcatca
     2221 acgacatcct ggacctctcc aagatcgagg cggggcgcat gaccctggag cgcgtcgact
     2281 tcgacctgcc ggcccacctg gcccaggtgc tcaagacctt caagccggcc ttcgcgcgga
     2341 agggcctcta cctggagttc gaggcggcgc cggggagccc ccgcttcgtc gcgggcgact
     2401 cgctcaggct cggccaggtg ctcatgaacc tcgtgggcaa cgcggcgaaa ttcacggaga
     2461 agggcggcgt gcgggtgagg ctcgaggccg aggcggctgg ggaggggaag gcggggatcc
     2521 gcgtcacggt cgccgacacg ggcatcggca tcccgcccga caagctcgac ctcatcttcg
     2581 agagcttctc ccaggccgac tccagcgtga gccggcgcta cggcggcacg gggctcggcc
     2641 tctcgatctg ccgcaacctc gtgggcctct tcggcggccg catctgggtg gagtccgagc
     2701 cgggcaaggg ctcgaccttc tccttcacgg ccttcctgga gcccggcgac gaggcgaagg
     2761 ccgccgccag gatccggccc ggcgaagggg agggagaggc cgcggggggc gggcccttcg
     2821 acatcctcct cgcggaggac aacctcatca acgcgaaggt ggccctgcgc tggctggagc
     2881 aggccggcca ccgggcgcgg cacgcggtct ccggggcgga ggcgatccgc cttctctcgg
     2941 agcggccctt cgacctcgtc ctcatggacg tcgagatgcc cgacatcgac ggcctggagg
     3001 cggcgcggcg cgtcaggggc ggcgccgccg ggctggaccg ccgcgccgtg cccatcatcg
     3061 cgatgacggc ccacgacgac caggccatgc gcgaggcctg cgccagggcg gggatggacg
     3121 actacgtgac gaagcccgtc gacttcgtgg agctctcagg cgcgctcgcg agggtggcgc
     3181 gtccttcgac cagggaggcg gcggcgaagg ccccggccct ggtccccgcc tcgggcgagc
     3241 ccctcgccgc cgcgcccctc ctggacgagg ccgagcccct gcgccgcctg ggcggggacg
     3301 gcgacctcta cctggagctg atctccatct tcgccgccga ggcccggggc aggcgggagg
     3361 ccctggaggc ggcgctcgcg gcccgcgagc ccgagaccct ccgccgcctg gcccacagcc
     3421 tccgcggcgg ggcgcgcacc atcggggccg agcccctggc ggcggcggcc gccgccctgg
     3481 agaaggcggc cggggaagcc gactcctccg aggccggcgc ggcctcggcc atgcccgggc
     3541 tcgagaggct ccaggcggcg gccctcgtcc tcctcgactc cacggccagg cggatcggcc
     3601 agcgcctccc tcccggctac gaggctcccc aacccgagta ggcttccggg cgaaatgagc
     3661 ttccgcggaa ggccggacgc gggtatactt ccgccctatg gcgagaagca gtcggctctt
     3721 cccgggaatc ccctcgcggg gggactccgc gccggcgggc aggactcccg cggccgggag
     3781 accggcaggc tcccggtgga ggccctgggc cgagggggcc gtggccctcc tcttcctcct
     3841 cgtcttcgcc gtcttcgccc agtccctcct ggacgccca
//
LOCUS       DAOOSJ010000243         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_15036_length_3871_cov_5.713574, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000243 DAOOSJ010000000
VERSION     DAOOSJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15036_length_3871_cov_5.713574"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLG14_10600"
     CDS             <1..667
                     /locus_tag="PLG14_10600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX49624.1"
                     /translation="GDAVLDACCGVGRHSIELAKRGYRVTGVDLTGPYLDAARESAEA
                     EGVAPEFLKADIRTFSRPGAYDLALNLFTSFGYFATPEEDLAALRALRSSLAPGGSLV
                     IETLGKETAVRDFIEGEWFERGGWTVLTEFSVDGAWEGLRTRWVLLRGAERIDRSFVQ
                     RLYSGVEMRKALLQAGFAEVEIYGSVEGAPYDEKAGSLVALARVGPSGRAAGAAEKAG
                     ADS"
     gene            806..1795
                     /locus_tag="PLG14_10605"
     CDS             806..1795
                     /locus_tag="PLG14_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX49625.1"
                     /translation="MTKNKIGLKLANGEFYPVLEDGASARKRLVVTTVQDGQRSVQID
                     LYRGAGPTIIDAAYIGSLVIENIPLAPRGEPDIRLDLGLSADGTLEAFAENVASGERQ
                     SLSVSLESLAEEEKYEIPDFRFEEEATDQLPEEESAPLEEEPAWSAPRREAEGEGLLA
                     RASGVRPEEEAGAPRSRKPLYIALAVLSAILVALGLAYLVYSLGMSRNGGAVMAEEPA
                     AAPAVIPVEPAPAPPAPAPAPAAAATPAPAAASTPAPAPVPAPAPAAAPAKPAPAAKQ
                     PGVNYTIRWGDTLWDLAYAYYRNPWLYPKIAKANGIKNPDYIISGRKIYIPPR"
     assembly_gap    2103..2202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2203..>3871
                     /locus_tag="PLG14_10610"
     CDS             <2203..>3871
                     /locus_tag="PLG14_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49626.1"
                     /translation="ALGPLLFASCEEEYAFGLGEGELKRMLGEGDAAAFMALAEGSRE
                     KPGKMDPEAYYYLAMWLEAKSQAAARAAAAPAGSAPAPEAAPPQAPPPENGKALEPKP
                     ESELPPALEGLGAPAAILPPQASKASADPRALALDFLRLAFERSSGYARREAGWALVE
                     RLAARAAEAAAGPAAKAAHEEVLAAADAFGQEFGPDWRIRRARLEALDALGRDAELLR
                     ETLGLRSGFPLEAAADADALLYYEGSASRRLGKKAWAAPLRALLLERPASDWTAKALA
                     FVESLEPEAGAAPAAASRGFQAAELHAARLRLEVRRRDYGAAYRAAARCLDLVLSPST
                     PRHLLADAGKAFLYSGSSKEGLVRFERAFGTAYPGAAATAAPAEAPAKAAGAEAGAAR
                     PGQAADPAEATAWTAAFYRARFMKALGRDAEASALFKGLAPLAPGPEDLDAALWYGAD
                     AAMKAAKAEADAAGKTKSALKLSAAAREKAALKRAAGLRRLSLDVLAEASRSWRNPLS
                     FADLADALFREAIAARDWELVADFASRLGPRLTPTLGARAAYAAGRAL"
BASE COUNT          481 a         1516 c         1314 g          460 t
ORIGIN      
        1 cggcgacgcg gtcctggacg cctgctgcgg cgtcggccgc cactcgatcg agctcgccaa
       61 gcgcggctac cgcgtcacgg gcgtcgacct cacgggaccc tacctcgacg ccgcccgcga
      121 gagcgcggag gccgaggggg ttgccccgga gttcctcaag gccgacatca ggaccttctc
      181 ccggccgggg gcctacgatc tcgcgctcaa cctcttcacg agcttcggct acttcgcgac
      241 cccggaggag gacctggccg ccctccgcgc cctccgctcg agcctggcgc ccggcgggag
      301 cctcgtcatc gagacgctcg gcaaggagac ggcggtcagg gacttcatcg agggcgagtg
      361 gttcgagcgg ggcggctgga cggtgctcac ggagttctcc gtcgacggcg cctgggaggg
      421 gctcaggacc cgctgggtcc tcctccgggg cgccgagcgg atcgaccgct ccttcgtcca
      481 gcgcctctat tccggcgtcg agatgcggaa ggccctcctt caggcgggct tcgccgaggt
      541 cgagatctac ggcagcgtcg agggcgcgcc ctacgacgag aaggccggga gcctggtcgc
      601 cctggccaga gtcggcccga gcggccgggc cgccggagcc gcggagaagg ccggcgccga
      661 cagctaacgc acggcggctg gcgcgcggga cggttgattt tccggcgcgg gtggctacac
      721 ttaggagtac gggccgggga aacgcgccgt ttcgcgcgtc gcgcctagcg cgcggcccga
      781 caagcacgac cgatcggaag gcgccatgac gaagaataaa atcgggctca agctcgccaa
      841 cggcgaattc tacccagtcc tcgaggacgg cgcctccgcg aggaagcgcc tcgtcgtcac
      901 gaccgtccag gacggccagc gcagcgtcca gatcgacctc taccgcggcg cgggcccgac
      961 catcatcgac gcggcctaca tcggcagcct cgtcatcgag aacatccccc tggccccccg
     1021 cggcgagccc gacatccgcc tcgacctggg cctctccgcg gacggcaccc tggaggcctt
     1081 cgccgagaac gtcgcctcgg gcgaaaggca gagcctctcg gtctccctgg agtccctggc
     1141 cgaggaggag aagtacgaga tacccgattt ccgcttcgag gaggaggcga cggaccagct
     1201 ccccgaggag gagtcggcgc ctcttgagga ggagcccgcc tggtcggccc cccgccgcga
     1261 ggccgagggc gagggccttc tggcccgcgc gagcggcgtc aggcccgagg aggaggccgg
     1321 cgcgccccgg agccggaagc ccctctacat agccctggcc gtcctctccg cgatcctcgt
     1381 cgccctcgga ctggcctatc tcgtttactc cctcgggatg tcgcggaacg gcggcgccgt
     1441 gatggccgag gagcctgcgg ccgcgcccgc ggtgatcccc gtcgagccgg ctccggcgcc
     1501 cccggcgccc gctccggcgc ccgccgccgc cgcgacgccg gcccccgccg cggcgtcaac
     1561 gccggccccc gcgcccgtcc cggctcccgc tccggcggcc gcgccggcca agcccgcgcc
     1621 ggcggccaag cagcccggcg tcaactacac gatccgctgg ggcgacacgc tctgggacct
     1681 cgcctacgcc tattaccgca atccctggct ctacccgaag atcgccaagg cgaacggcat
     1741 caagaacccc gactacataa tctcgggccg caagatctac ataccgcccc gctgatacgc
     1801 cggacggcgc ctcggtccgt tccgaggcgc cgacctgaag gccccacggc gccgccgggc
     1861 cgatccgccg cgccgcccgt cgtttccgag gcgccggccg agggccccgc ggcgccagcc
     1921 ttccccgctc cccttcgggg actggaagcc gggcgccgcc cgatgctagg cttccggcca
     1981 gccatgaaca gcgcccaagc cccagcgcgc ggcccccgcc gcgctgccgc cctcctcatc
     2041 cccctcttcg ccctgggccc cctcctcttc gcgtcctgcg aggaggagta cgccttcggc
     2101 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcgccctg ggccccctcc
     2221 tcttcgcgtc ctgcgaggag gagtacgcct tcggcctggg cgagggcgaa ctcaagcgca
     2281 tgctgggcga gggcgacgcg gccgccttca tggccctggc tgagggttcc cgggagaagc
     2341 ccggcaagat ggatcccgag gcctactatt acctcgcgat gtggctagag gcgaaatccc
     2401 aggcggccgc ccgggcggcc gcggcacccg caggctcggc ccccgccccc gaggcggcgc
     2461 ctccccaggc cccgcccccc gagaacggga aggcgctgga gcccaagccc gagagcgaac
     2521 tgccccccgc cctggagggc ctcggcgcgc cggccgccat ccttccgccc caggcgtcca
     2581 aggcctcggc cgatccccgc gccctcgccc tggacttcct caggctcgcc ttcgagcgct
     2641 cctcgggcta cgcgcgccgc gaggccggct gggccctcgt ggagaggctc gcagcccggg
     2701 cggcagaggc cgccgccggc cccgcggcca aggccgcgca cgaagaggtc ctggccgcgg
     2761 ccgacgcctt cggccaggag ttcggccccg actggcgcat ccgccgcgcg cggctcgagg
     2821 ccctcgacgc cctgggccgc gacgccgagc tcctccgcga gaccctcggc ctccgctccg
     2881 gcttccccct ggaggccgcg gccgacgcgg acgccctcct ctactacgag ggctcggcct
     2941 cccgccgcct cggcaagaag gcctgggccg cgcccctccg cgccctcctc ctggagcgcc
     3001 cggcctccga ctggacggcc aaggccctcg ccttcgtcga gtcgctggag ccggaggcgg
     3061 gggccgcgcc ggccgccgcc tcgcgcggct tccaggccgc ggagctccac gcggccaggc
     3121 tgcgcctgga ggtgcggcgg cgggactacg gcgccgccta tcgcgccgca gcgcgctgcc
     3181 tggacctcgt cctctcgccc tcgacgccgc gccacctcct ggccgacgcg ggcaaggcct
     3241 tcctctactc gggctcctcg aaggagggcc tcgtccgctt cgagcgggcc ttcgggacgg
     3301 cctatcccgg ggccgcggct acggccgcgc cggcggaggc gcccgccaag gcggcgggcg
     3361 cggaggctgg cgcggcgagg ccgggccagg cggccgaccc cgcggaagcg acggcctgga
     3421 cggccgcctt ctaccgcgcg cgcttcatga aggccctggg gcgcgacgcg gaggcctcgg
     3481 ccctcttcaa gggcctcgcc ccgctggcgc cgggcccgga ggacctggac gcggccctct
     3541 ggtacggggc cgacgcggcg atgaaggccg ccaaggcgga ggccgacgcg gcgggcaaga
     3601 ccaagagcgc gctcaagctc tcagccgcgg ctagggagaa ggcggcgctg aagcgggcgg
     3661 cgggactccg ccgcctctca ctcgacgtcc tcgccgaggc ctcgcgctcc tggcggaatc
     3721 ccctctcctt cgccgacctg gccgacgccc tcttccgcga ggcgatcgcg gcccgcgact
     3781 gggagctcgt cgcggacttc gcctcgcgcc ttgggcccag gctgacgccg accctcgggg
     3841 ccagggccgc ctacgcggcg ggccgcgccc t
//
LOCUS       DAOOSJ010000244         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_15219_length_3837_cov_6.883131, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000244 DAOOSJ010000000
VERSION     DAOOSJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_3837_cov_6.883131"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLG14_10615"
     CDS             complement(<1..261)
                     /locus_tag="PLG14_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX49627.1"
                     /translation="MTPEIIAADPAWPYTKAKTSVLSAAAAVIREDGPRAATLKNVAS
                     RAGITEPAIFRHFEGIDGLFCGLFETVERIYDRVAASFQTEER"
     gene            complement(318..1796)
                     /locus_tag="PLG14_10620"
     CDS             complement(318..1796)
                     /locus_tag="PLG14_10620"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX49628.1"
                     /translation="MKFLKKLLPFAVILAAALLLLYLPDIGPERYTDLAAFFRTELKR
                     QGFSGYTVAVVKDGSVLYVDAFGTDGQGEALTVDTPMAIGSLSKSFTGLAALSLAREG
                     KLDLDAPVGAYLPWFSFAPGGEGGDGSGVLLRHLLSHTSGASDLDFDDAHPSAPDLES
                     AVRSLAGARARALPGAERHYLNTGYQALGLVLEKASGQPYSSLVASRILKPLGMEKSS
                     AEPDKVSGALPYGNGSFFGAVLPRRQVLPAFSAPSGGIVSTAEDMAKYLAFLAAPEKK
                     KRPPLSAKAVPSLFAPLAPASAYGYGWRVAGEGPDRRAEHGGSLEGFSAQAVVWPERR
                     AAFVVLAAQNSLLHSTVGMPALAEGAGRLILEGEAPRPFPLGRLFILLAVAAAVHLLA
                     LAVQTGAALRWAREVKGRAEALGAKGPIVFARVRCAFGVALRIALVAVAPALLTAAFR
                     RKVGWALAFQLEPGLAAWAVSACAFGCLRNLTRLAWLRGPRR"
     gene            2120..3466
                     /locus_tag="PLG14_10625"
     CDS             2120..3466
                     /locus_tag="PLG14_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOX49629.1"
                     /translation="MSGKKGFWKLLGFRYGDDRDLGDGLGGLLPDEAPGLGRLVLFAI
                     QQFIVMFPATVLVALLTGFHVSTTIFASGLATLSFILVTGGKIPLYYGSSFSYIAAIV
                     SLTGAREFGALAPDALIGEAQFGIILSGLVSILVGLLIERFGHDKVEKVLPAEVTGPI
                     AIVIGLSLAGTAMQGAAQNWGVALVTLVVTMAASVFLRGFLAQLPVLIGIAAGYLAAL
                     AAGLVDFGGVASAAAIQAPHFTLPVPSWAAVAAIMPIAIATIPESTAHLYQIDLYVNK
                     LAAKRGEKKYEISDKLGRNLIGDGVGDIVSSLFGGPAGTNYGENISTMAVTRVFSVPV
                     LVATAVLAILVSFLGKFAAAVNTIPGAVIGGLSVYLFGVIAVQGVGIMVEKKVDLFDN
                     RGLAVIATILVIGIGGSFAFPGGMIPVFGAKLPAIATAAIWGILLNLVLRVTRKKG"
BASE COUNT          555 a         1406 c         1319 g          557 t
ORIGIN      
        1 ccgctcctcc gtctggaagg aggcggcgac gcggtcgtag atccgctcca ccgtttcgaa
       61 aaggccgcaa aagagtccgt cgatgccctc gaagtggcgg aagatcgccg gctcggtgat
      121 gccggcccgg gaagcgacgt tcttgagggt cgcggcccgg gggccgtcct cgcggatgac
      181 cgcggcggcc gccgagagga cggaggtctt cgccttcgtg tagggccagg cgggatcggc
      241 ggcgattatt tccggcgtca tatatacatt atatccaatc gctctggaac gaggaagccg
      301 caatcggcaa aaaatctcta gcggcggggg ccgcggagcc aggctaggcg ggtcaggttc
      361 cggaggcagc cgaaggcgca ggcgctgacg gcccaggccg cgaggccggg ctccagctgg
      421 aaggcgaggg cccagcccac cttgcggcgg aaggccgcgg tgaggagggc cggcgcgacg
      481 gccacgaggg ctatcctgag ggccacgcca aaggcgcagc ggacccgcgc gaagacgatg
      541 ggtcccttgg cgcccagggc ctcggcgcgg cccttgacct ccctggccca gcggagggcc
      601 gcccccgtct ggacggcgag ggccaggagg tggacggcgg cggcgacggc gaggaggatg
      661 aagagcctgc ccagggggaa gggccgcggc gcctcaccct ccaggatgag gcgtcccgcc
      721 ccctcggcca gggccggcat gccgacggtc gaatgcagga ggctgttctg cgccgccagg
      781 acgacgaagg ccgcgcggcg ctccggccat accaccgcct gggcggagaa gccctccagc
      841 gagcctccgt gctcggcgcg cctgtccggg ccctcccccg ccacgcgcca gccgtagccg
      901 taggcggagg cgggcgcgag aggagcgaag aggctcggca cggccttcgc ggaaaggggc
      961 gggcgcttct tcttctcggg cgcggccagg aaggccaggt acttggccat gtcctccgcc
     1021 gtggagacta tgccgcccga gggggccgag aaggcgggca agacctggcg ccgcggcagg
     1081 acggccccga agaaggagcc gttcccgtaa ggcaaggcgc ccgagacctt gtcgggctcg
     1141 gccgagctct tctccatccc gaggggcttg aggatccgcg aggcgacgag gctcgagtag
     1201 ggctggcccg aggccttctc cagcaccagc cccagggcct ggtaacccgt gttgaggtag
     1261 tgccgctcgg cgccgggaag ggctcgcgcc ctggcccctg ccaagctccg caccgccgac
     1321 tcgaggtcgg gggcggaggg atgggcgtcg tcgaaatcga ggtcgctcgc cccgctcgtg
     1381 tgggagagga ggtggcggag gaggacgccg gagccgtccc cgccctcgcc gcccggcgcg
     1441 aaggagaacc agggaaggta ggcgccgacc ggggcgtcca ggtccagctt gccctccctg
     1501 gccagggaaa gggcggcgag gcccgtgaag gacttggaca gggagccgat cgccatcggc
     1561 gtgtcgacgg tcagggcctc gccctgaccg tccgtcccga aggcgtccac gtagaggacg
     1621 gagccgtcct tgacgacggc gaccgtgtag cccgagaagc cctgccgctt cagctccgtg
     1681 cggaagaagg ccgcgagatc cgtgtagcgc tcggggccga tgtccggcag gtagaggagg
     1741 agaagggccg cggccaggat gaccgcgaag gggaggagct tcttcaggaa cttcatcggc
     1801 ctacaactat agcataggac gatacttccg tctcgcggcc ccggccggcg cggtcctggc
     1861 cggcgcggtc ctggcccgcg ctgaccaggg ccggcgcggg gccgcgcgga gccggaggcc
     1921 tcgccggcgc ggcccccttg ccagcggccg ggaggggatg ctatacagag gcccaagatg
     1981 cgaaaccatg gcgcccccgc gggcactcgg cccgacttgg accgaactag tcatttttag
     2041 gaccgtccct ccggacggtc ctttttttat gccttccgtg cccatcggca cccgggcccg
     2101 tcggcccaaa ggagagtaca tgagcgggaa aaagggattc tggaagctgc tcggcttccg
     2161 ctacggcgac gacagggacc tgggagacgg cctgggcggc ctcctccccg acgaggcccc
     2221 gggcctgggc aggctcgtcc tcttcgccat ccagcagttc atcgtgatgt tcccggcgac
     2281 cgtcctcgtc gccctcctca ccggcttcca cgtctccacg acgatcttcg cctccggcct
     2341 cgcgaccctc tccttcatcc tggtcacggg cggcaagatc cccctctact acggctcctc
     2401 cttctcctac atcgcggcca tcgtctccct gaccggggcc cgcgagttcg gcgccctcgc
     2461 gccggacgcc ctcatcggcg aggcccagtt cggcatcatc ctgtcgggcc tcgtctccat
     2521 cctcgtcggc ctcctcatcg agcgcttcgg ccacgacaag gtcgagaagg tcctcccggc
     2581 ggaggtgacg ggcccgatcg cgatcgtcat cggcctctcc ctcgccggca ccgcgatgca
     2641 gggggcggcc cagaactggg gcgtcgccct ggtcaccctc gtcgtcacga tggccgcctc
     2701 cgtcttcctc aggggcttcc tggcccagct ccccgtcctc atcggcatcg cggccggcta
     2761 cctcgcggcc ctggcggccg gcctcgtcga cttcggcggc gtcgcctccg cggcggccat
     2821 ccaggccccg cacttcaccc tgcccgtgcc ctcctgggcc gcggtcgccg ccatcatgcc
     2881 catcgccatc gcgaccatcc ccgagtcgac cgcccacctc taccagatcg acctctacgt
     2941 caacaagctg gcggccaagc gcggcgagaa gaagtacgag atctccgaca agctcggccg
     3001 caacctcatc ggcgacggcg tgggcgacat cgtgagctcc ctcttcggcg gccccgccgg
     3061 cacgaactac ggcgagaaca tctccacgat ggcggtcacc cgcgtcttct ccgtgcccgt
     3121 cctcgtcgcc accgccgtcc tcgccatcct cgtgagcttc ctcgggaagt tcgccgcggc
     3181 ggtcaacacc atcccgggag ccgtcatcgg cggcctctcc gtctacctct tcggcgtcat
     3241 cgccgtccag ggcgtcggca tcatggtgga gaagaaggtc gacctcttcg acaaccgcgg
     3301 cctcgcggtg atcgcgacca tcctcgtcat cggcatcggc ggctccttcg ccttcccggg
     3361 cggcatgatc ccggtcttcg gcgccaagct ccccgcgatc gccaccgcgg ccatctgggg
     3421 catcctcctc aacctggtcc tccgcgtcac gcggaagaag ggctaagggg ggagcgcccg
     3481 ccgggcgctc ggtccgcgcg gccccctcgg gctttccatg agcccgtcgg ggcggggcct
     3541 ggcggcgcgg acgagatccc cgctcggagc tcgcggggac cttcgtccgc gccgccaccc
     3601 gccgccttgc cccgctccgg ggaagcgcgg gcgggtacaa tcgtcccggg cccgccgcca
     3661 ggcgccggcc ccttcgccct tgcccgcgcg gacctcgcga agggcgcggc cgcggaaccg
     3721 ccgctcggcc tgacgcggct ccccctcgct ccgaaagtcg cgctacgcgc gacgtttctc
     3781 cgctaccccc actcttgttt tacagcctca ttgacgccag gttgaattcc cgcggcg
//
LOCUS       DAOOSJ010000245         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16202_length_3661_cov_6.128120, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000245 DAOOSJ010000000
VERSION     DAOOSJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16202_length_3661_cov_6.128120"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..863)
                     /locus_tag="PLG14_10630"
     CDS             complement(270..863)
                     /locus_tag="PLG14_10630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOX49630.1"
                     /translation="MELLVATGNAHKLEELKPLFPGHRLLKPADVGHAGFDVLEDGTT
                     FLENAMKKALALHALTGRPSLADDSGLAVRALGGAPGIYSARYGSEDGSRKLEAAERN
                     AYLLSNMEGKTDRACAFVCCLVLVLSQDRFFAVQESLEGELLHAPRGSGGFGYDPVVF
                     LPGLGRSVAELSAEEKNRLSHRGKASARMAALLSTLD"
     gene            complement(1038..>1880)
                     /locus_tag="PLG14_10635"
     CDS             complement(1038..>1880)
                     /locus_tag="PLG14_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49631.1"
                     /translation="ELYRDPGRFGFEGEDDAAEALERHRERILGLAGRYVDRGAPFEA
                     YLAVSLRFLARSMRRERRRSKERELVCERAETWDAEARAVEAAEGTERPHAAEGEAAA
                     GTESAPRPAASRSSSAAALKSRLVFLYLKCAWEADDAETSRVAEAAGVPSDWLAAAAG
                     QSRRFLETERRRFERLRARRDRSWSRLRLLEGRLASECEPPARERLSASIARERENFR
                     RAREAIARFKPIVPNSVVAKILGVPKGTVDSGLYYLKRLERGRAGQAVPAESRPKAAA
                     CSAR"
     assembly_gap    1881..1980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2105..3082)
                     /locus_tag="PLG14_10640"
     CDS             complement(2105..3082)
                     /locus_tag="PLG14_10640"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOX49632.1"
                     /translation="MKRVPPELLDFIDRHGTFYVVGHKEPDGDCVGSQLALASFLRRN
                     GKDVFPLSAGPFTRTEILSYEHRFTDIAPAERASERAAAIILDCSSLQRIGSVAETLP
                     SVPLAFIDHHASGEASGEVVFIDDRAPAVTLMVLALIEAMGQKPTKEEAELLLFGLIT
                     DTGFFRHLDEGSAETFAAASRLVAAGASPKRGFSMMYGGKSLYSRKLMGEILTRTEPY
                     FEDRLLISYVTLDDQQRYGLASRDSDMLYQLLMSVAGCEAAMIIRQERETDCTVGLRA
                     RDRVDVGSIAAVFGGGGHRLAAGLSIRGRIEDVRDRLLEAFAPAFADLD"
     gene            complement(3181..3570)
                     /locus_tag="PLG14_10645"
     CDS             complement(3181..3570)
                     /locus_tag="PLG14_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49633.1"
                     /translation="MKRALLAFGLVALVAAAAVSADDSAFYPLRLDVVKVYSHADGYR
                     VVYRKGTSGMAELYMPIKWFVPGGKAELVRANDPSFPYLVVYYKEGKFSHARLYAQAN
                     QKDPTWGILDPAEGKGKFELDELKPEF"
BASE COUNT          516 a         1181 c         1338 g          526 t
ORIGIN      
        1 tgccggtgcc caagaccccg aacctggacc tcgcgatcct cttcgcgacc gtgccggttc
       61 gcgagtcgga cggctcgatc aggtggaagg acaacaaccc cgccgcgggc atccccgagg
      121 tgaagccttc catctccatc gagacgaggc tccacttcta gccagacacg ccagcgtatg
      181 cgaaaggggc tgcccgggag ggcagcccct tcttattggc ggcgcgtcgt gcgggcgcgg
      241 ccgaaggccg cgcggatccg gccgccggcc tagtccagcg tggagaggag ggcggccatc
      301 ctggcggagg ccttgccccg gtgggagagg cggttcttct cctccgcgga gagctcggcg
      361 acgctccttc ccaggccggg caggaagacg acgggatcgt agccgaagcc gcccgagccc
      421 ctcggcgcgt ggaggagctc gccctccagg ctctcctgga cggcgaagaa gcggtcctgc
      481 gaaaggacaa ggaccaggca gcagacgaag gcgcaggccc tgtccgtctt gccctccatg
      541 ttggacagga gataggcgtt gcgctcggcc gcctcgagct tccggctccc gtcctccgag
      601 ccgtagcggg ccgagtagat gcccggcgcc ccgcccaggg cgcgcacggc gaggcccgag
      661 tcgtcggcca gggagggacg cccggtgagg gcatggaggg ccagggcctt cttcatcgcg
      721 ttctcgagga aggtggtgcc gtcctccagg acgtcgaagc ccgcgtggcc gacgtcggcc
      781 ggcttgagca ggcggtggcc ggggaagagg ggcttaagct cctcgagctt gtgggcgttg
      841 ccggtggcga cgagcagttc catgggagca ccatagccgg aaggcgcgga ccgcggcaag
      901 cggcgcgggc cgaggcctga cgcgggcgag ctcgggccgc ggcaagcggc gggccacgcc
      961 ccgctcacct cgcctggcgg cgcgccgcgt cgaccgctcc gccgctccgc ccccgccgcg
     1021 ccccggcgcg gcctcgccta tcgcgccgag caggccgccg ctttcggcct ggactccgcc
     1081 gggacagcct ggccggccct cccgcgctcg agccgcttca ggtagtacag gcctgagtcc
     1141 accgtgccct tgggcacgcc caggatcttc gcgacgacgg aattgggcac gatgggcttg
     1201 aagcgcgcga tcgcctccct tgcccgcctg aagttctccc tctcacgcgc gatggaggcg
     1261 gagagccgct cccgcgctgg cggctcgcac tccgaggcga gcctcccttc aaggaggcgg
     1321 aggcgggacc aggagcggtc tcgcctcgcg cggaggcgct cgaagcggcg gcgctcggtc
     1381 tccaggaagc gccgggactg gccggcggcc gcggcgagcc agtccgaggg gactcccgcg
     1441 gcctcggcca cgcggctggt ctccgcgtcg tcggcctccc aggcgcactt caggtagagg
     1501 aagacgaggc ggcttttaag ggcggcggcg ctcgagctac gggaggccgc gggcctgggc
     1561 gcggactcgg tcccggccgc cgcctcgcct tccgccgcgt gaggccgttc ggtcccttcg
     1621 gccgcctcga ccgcccgcgc ctcggcgtcc caggtctcgg cccgctcgca gacgagctcc
     1681 ctctccttgg agcgccgcct ctcccggcgc atgctccggg ccaggaagcg gaggctcacc
     1741 gcgaggtagg cctcgaaggg ggctccgcgg tccacgtagc ggccggccag gcccaggatg
     1801 cgctcccggt ggcgctccag ggcttcggcc gcgtcgtcct cgccctcgaa gccgaagcgg
     1861 cccggatcgc ggtagagctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 tcccgcctcg gggtttcttt gcctttcttt gcgttggccg gcttttcgcg ggctttccgc
     2041 ggcgcggcgg gccccggaaa tacccggggc ttaaggcccg tccgcggacc cttgcgggct
     2101 gggcctagtc caggtcggcg aaggcggggg cgaaggcctc gaggaggcgg tcccgcacgt
     2161 cctctatcct gccgcggatg gagaggccgg ccgccaggcg atggccgccg ccgccgaaga
     2221 cggccgcgat gctccccacg tccacgcggt cgcgggcgcg gaggcccacg gtgcagtccg
     2281 tctcccgctc ctggcggatg atcatcgcgg cctcgcagcc ggcgacgctc atgaggagct
     2341 ggtagagcat gtcggagtcg cggctcgcga ggccgtagcg ctgctggtcg tccagggtca
     2401 cgtaggagat gaggaggcgg tcctcgaagt agggctcggt gcgggtgagg atctcgccca
     2461 tgagcttccg ggagtagagg gacttgccgc cgtacatcat ggagaagccg cgcttgggcg
     2521 aggcgccggc cgcgacgagg cggctggctg cggcgaaggt ctcggcgctg ccctcgtcca
     2581 agtggcggaa gaagcccgtg tcggtgatga ggccgaagag gaggagctcg gcctcctcct
     2641 tcgtcggttt ctggcccatg gcctcgatga gggccaggac catgagggtc acggcgggag
     2701 cccggtcgtc gatgaagacg acctcccccg aggcctcgcc cgaggcgtgg tggtcgatga
     2761 aggcgagggg cacggagggt agggtctcgg cgacggagcc gatgcgctgg aggctcgagc
     2821 agtcgaggat gatggccgcc gcccgctcgg aggcgcgctc ggcgggggct atgtccgtga
     2881 agcggtgctc gtaggagagg atctccgtgc gcgtgaaggg gccggcggag agggggaata
     2941 cgtccttgcc gttgcgcctg aggaaggagg cgagggcgag ctgggagccc acgcagtcgc
     3001 cgtcgggctc cttgtggccg acgacgtaga acgtcccgtg tcgatctatg aaatcgagga
     3061 gctcgggggg aacgcgtttc atggaaagcc gcccttctgg tcgtaggtat ggcagggccg
     3121 tccgggaatg ctccccggac ggccgagcgg atcgagatcg gtcgatcgcg cgcgagtgta
     3181 tcagaattcc ggtttcagtt catccagctc gaacttgccc ttgccctcgg cgggatcgag
     3241 gatgccccag gtgggatcct tctggttcgc ctgggcgtag agccgggcat ggctgaactt
     3301 cccttccttg tagtagacga cgaggtaggg gaagctcggg tcgttggccc gcacgagctc
     3361 ggccttgccg ccgggcacga accacttgat cggcatgtag agctccgcca tgccggaggt
     3421 tcccttgcgg tagacgacgc gatagccgtc ggcgtgggaa tagaccttga cgacatcgag
     3481 cctgagggga tagaaggcgg aatcgtcggc cgagacggcg gcggccgcca ccagggcgac
     3541 gagtccgaag gcgagcagtg cacgtttcat gcgaccctcc gtttcgaaaa tgaagtataa
     3601 ggcctcgcgc gggcggccgc aagggaaaat cccggcccgg aagggaggcg atgggcgaaa
     3661 g
//
LOCUS       DAOOSJ010000246         3649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16280_length_3649_cov_3.577629, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000246 DAOOSJ010000000
VERSION     DAOOSJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16280_length_3649_cov_3.577629"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="greA"
                     /locus_tag="PLG14_10650"
     CDS             <1..733
                     /gene="greA"
                     /locus_tag="PLG14_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOX49634.1"
                     /translation="KEIENHRDTTENRKINKQIQTILFSEGALETFILDSDSDTVERV
                     YTLVADVKDLDPAIKMRLRRNIMDKWPEFKVSGEEEKTVVSRGLMVTAAKYEEKQKLL
                     QEILETEVPANQKEIAYALSLGDLRENAEYKAAKEKQDELNAKVAKLKNEIERAQIFD
                     KSTLNASKIAFGTIATLVNEATGETETYTILGPWESEPSQNVISYLSPLGKKLLNHKP
                     GESLSFLIHERKFQYKVERIETVKL"
     gene            760..>2404
                     /locus_tag="PLG14_10655"
     CDS             760..>2404
                     /locus_tag="PLG14_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX49635.1"
                     /translation="MRKSLAAAALIAAALLAGPGAYAGSSSVATEGESEDVIVLLDVS
                     QSVLPYFQEVTDYVVGSVVRDFLRLGDTFHLLTFGDEVQVEIAQRLSDERDVKSVLGK
                     LYLLYPLARSTDLVSAFGYLYQYVADLPLSRGKVVVIITDGVHNPAAGGASAGLSPEA
                     VRSELDAVAAKIRRDGWPVYIIKIPFSAERAGAETAAVQSGAKASAPGAAGGVPASPS
                     GAAGSGSSGAAAGSAAASGAAGAAAQAPGSQAQPTDYVGDLGRSLGATVTEYSSEGKE
                     DLAKRSLSLPEIEFPGPLGKRGYAFSFPLKVTNRSDSDLGLELERVSSGSSELLEKKS
                     FLSLGPGKSGVMKVSVRLPEGTEPGPLRLPVELFFADGLRAAPSRGVLELTLAPSFLS
                     ALFRSGARALLFALLLAIGLGAALFAVILVRRAPRRAAAPIVAAVRESSEREAGPAAT
                     ARGGQAARPQAPGLPLKAASGAAPAAPSKPAQAPVRAATSPVAEVPAPAAELRPQPSR
                     AAKVPARREKAPVPSGYQPRVARPGTIQVELRVEDQNPHI"
     assembly_gap    2405..2504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2505..2894
                     /locus_tag="PLG14_10660"
     CDS             <2505..2894
                     /locus_tag="PLG14_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49636.1"
                     /translation="RPGTIQVELRVEDQNPHIGSRNVHTLHAGSAKAVGGGRSDYLVF
                     LVPVPGGSAELHFDGERCVFVPLRPELFPGLEGPLEDCLGKDISMVSRSGYPLVLRFQ
                     KYERPADRINRLLHCIEAPGLFSDLDP"
     gene            complement(3139..>3649)
                     /locus_tag="PLG14_10665"
     CDS             complement(3139..>3649)
                     /locus_tag="PLG14_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49637.1"
                     /translation="KRGLAEAIPILEYKAAKDPEKAVKTEAFKALAELGAAPGNDRCF
                     DFMKKYFEDPKSELVNRALVFGLLARKDPSSMGWLAGKLADAAKEKERSFYTALAREV
                     SAAADAKEAAPLARVLLADKDYLFRLGGLEWARKAKSAELRGDLAALAEKDPSEVIRK
                     KAAEILASY"
BASE COUNT          543 a         1360 c         1143 g          503 t
ORIGIN      
        1 caaggagatc gagaaccacc gcgacacgac cgagaaccgc aagatcaaca agcagatcca
       61 gaccatcctc ttctccgagg gcgccctgga gaccttcatc ctggactccg actctgacac
      121 ggtggagcgg gtctacaccc tcgtggccga cgtgaaggac ctggaccccg cgatcaagat
      181 gcgcctccgc cgcaacatca tggacaagtg gcccgagttc aaggtctccg gcgaggagga
      241 gaagacggtc gtctcccgcg gcctcatggt caccgccgcg aagtacgagg agaagcagaa
      301 gctcctccag gagatcctgg agaccgaggt cccggccaac cagaaggaga tcgcctacgc
      361 cctctccctg ggcgacttgc gcgagaacgc cgagtacaag gccgccaagg agaagcagga
      421 cgagctcaac gcgaaggtcg cgaagctcaa gaacgagatc gagcgcgcgc agatcttcga
      481 caagtccacc ctcaacgcct ccaagatcgc cttcggcacg atcgccaccc tggtcaacga
      541 ggccacgggc gagacggaga cctacacgat cctcggcccc tgggagtcgg agccctcgca
      601 gaacgtcatc tcctacctct cccccctggg caagaagctc ctgaaccaca agcccggaga
      661 gagcctcagc ttcctcatcc acgagcggaa gttccagtac aaggtcgagc ggatcgagac
      721 ggtcaagctc taaggagcgc gggggccatg atgcgcaaca tgagaaaatc gctggccgcc
      781 gcggccctga tcgcggcggc ccttctcgcg ggccccggcg cctacgccgg gagctcctcc
      841 gtggccacgg agggcgagtc ggaggacgtc atcgtcctcc tggacgtgtc ccagagcgtc
      901 cttccctact tccaggaggt caccgactac gtcgtcggct ccgtggtcag ggatttcctg
      961 cgcctgggcg acaccttcca cctcctcact ttcggcgacg aagtccaagt ggagatcgcc
     1021 cagcgcctct cggacgagcg ggacgtcaag tccgtcctgg ggaagctcta cctcctctat
     1081 cccctggccc ggagcaccga cctggtctcc gccttcggct acctctacca gtacgtggcg
     1141 gacctccccc tgagccgggg caaggtcgtc gtgatcatca ccgacggggt ccacaacccc
     1201 gccgccggcg gagcctcggc cggcctctcc cccgaggccg tcaggtccga gctcgacgcc
     1261 gtcgcggcca agatccggcg cgacggctgg cccgtctaca tcatcaagat acccttctcc
     1321 gccgagaggg ccggggccga gaccgccgcc gtccagtccg gcgccaaggc ttcggcccca
     1381 ggcgcggccg gcggagttcc cgcctccccg tcgggcgcgg ccggaagcgg ctcctcgggc
     1441 gccgccgcgg gctccgccgc cgcgtccgga gcagccggcg ccgcggccca ggccccgggc
     1501 tcccaggccc agccgaccga ctatgtcggc gacctgggcc gatccctggg cgcgaccgtc
     1561 accgaataca gctccgaagg caaggaggac ctggccaagc ggagcctctc cctgcccgag
     1621 atcgagttcc cgggcccctt gggcaagcgc ggctacgcct tcagcttccc gctcaaggtg
     1681 accaaccgct ccgactcgga cctcggcctc gagctcgagc gcgtctcctc cggaagctcc
     1741 gagctcctcg agaagaagag cttcctgagc ctcggccccg ggaagagcgg cgtcatgaag
     1801 gtctcggtgc gcctccccga ggggacggag cccgggccct tgcggctgcc ggtggagctc
     1861 ttcttcgccg acggcctcag ggcggcgcct tcccgcggcg tcctcgagct caccttggcg
     1921 ccgagcttcc tctccgccct cttccgctcg ggggcccgcg ccctcctctt cgcgctgctc
     1981 ctggccatcg gcctcggagc cgccctcttc gcggtgatcc tcgtgaggag ggcgccgcgc
     2041 cgcgccgccg cgcccatcgt ggctgccgtc agggaatcct ccgagcgcga ggccggcccg
     2101 gcggcgaccg cccgcggcgg ccaggccgcg aggccccagg ctccgggact gccgctcaag
     2161 gcggcctccg gcgccgcgcc cgccgccccc tcgaagcccg cccaggcgcc ggtccgcgcc
     2221 gctacatccc ccgtcgcgga ggtacccgcg cccgcggccg aactccgtcc ccagccttcc
     2281 cgcgccgcca aggtgcccgc caggagggag aaggcccccg ttccctccgg ctaccagccg
     2341 cgcgtcgcca ggcccggcac catccaggtc gagctccgcg tcgaggacca gaacccccac
     2401 atcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggccc ggcaccatcc
     2521 aggtcgagct ccgcgtcgag gaccagaacc cccacatcgg atcgcgcaac gtgcacaccc
     2581 tgcacgccgg ctcggccaag gcggtgggcg gcggccggag cgactacctc gtcttcctcg
     2641 tgcccgtgcc cggcggctcc gccgagctcc acttcgacgg cgagcgctgc gtcttcgtcc
     2701 ccctgcgccc cgagctcttc cccggcctcg agggcccgct cgaggactgc ctcggcaagg
     2761 acatcagcat ggtctcccgg agcggctatc ccctcgtgct gcgcttccag aagtacgagc
     2821 gccccgccga ccgcatcaac cgccttctcc actgcatcga ggcgcctggc ctcttttccg
     2881 acctcgatcc ctgatcgacg atccatcgaa ttgcgaagga gccgccccgc gtggggcggc
     2941 tccttttttg ccggcgtggc ccaggctcag gcgggcgcgc gcggccggga cggcgtcagg
     3001 ccggggcgcc gccctgccgt acaggcgacg cgcggccggg ccggcatcgg gtcgggcgcc
     3061 gcggacctgc gtcgcggcca agagcggcga agcacaacgg cccggggccg ctgcgccagg
     3121 gccgcggcgg gagaggtttc agtaggaggc gagaatctcg gccgccttct tgcggatgac
     3181 ctcggagggg tccttctccg ccagggcggc caggtcgccc ctgagctcgg ccgacttggc
     3241 cttcctggcc cactcgaggc cgcccagcct gaagaggtag tccttgtccg cgagcagcac
     3301 gcgcgcgagg ggagcggcct ccttggcgtc ggcggccgcg gagacctccc gcgccagggc
     3361 cgtgtagaag gacctctcct tctccttggc cgcgtcggcg agcttgcccg cgagccagcc
     3421 catggaggag gggtccttcc gcgcgaggag gccgaagacg agggcgcggt tcacgagctc
     3481 cgacttcggg tcctcgaaat acttcttcat gaaatcgaag cagcggtcgt tccccggcgc
     3541 cgcgccgagc tcggcgaggg ccttgaaggc ctccgtcttc acggccttct cgggatcctt
     3601 cgccgccttg tactcgagga tcggtatggc ctcggcaagg ccgcgcttc
//
LOCUS       DAOOSJ010000247         3641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16328_length_3641_cov_5.757111, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000247 DAOOSJ010000000
VERSION     DAOOSJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16328_length_3641_cov_5.757111"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLG14_10670"
     CDS             <1..1262
                     /locus_tag="PLG14_10670"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOX49638.1"
                     /translation="AMSDAQSAAMSLADEAYAGSASFERLEAAVREVLGKGLVLPVHQ
                     GRAAEHIICSSLVEPGSVVPMNYHFTTTRAHVELAGGSIAELIDPAALDPAAEEPFKG
                     NMDVSRLEELLSGAAGARVPFVRMEASANLIGGQPFSLANLRAVSRAAKSRGVPLVLD
                     ASLLAENVLLIKRREEGCGGKSIAELFLECCSLADLVYFSARKLSSSRGGAIATDDPA
                     LFARMRDLVPLFEGFLTYGGISLRDVESMAVGLRESLDETVIGQSPAFIEHLARRLDA
                     AGVPVVLPPGALGVHVDARAFLPGLRAADYPAGALAAAFYIASGVRGMERGAISAGRD
                     ESGREVPADMELLRLAFPRRVFTLSQLRYAEDRLEWLFANRGLVGGLRFVEEPSTLRF
                     FTGRLDAVGDWPERLAAAFRAEFGEEV"
     gene            1363..1950
                     /locus_tag="PLG14_10675"
     CDS             1363..1950
                     /locus_tag="PLG14_10675"
                     /inference="COORDINATES: protein motif:HMM:NF021295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49639.1"
                     /translation="MTVSMPPASAFERAAFYCEENVLRLLASPALAGLPAWALLVSNA
                     ARAVAMRGQAAGRGPAGLVVWDYHVLALAEAGGRLCALDLDTTLGFPLEAQAYVEAAF
                     PRGLSPEEEPRFRLVPSRDYVEGLASDRSHMRRSDGSWLAPPPPWDPPGAGKAPNLLS
                     WIDPEGEGPGQVLGLEELRSFARGRRPAAPAGSRP"
     gene            1947..>3641
                     /locus_tag="PLG14_10680"
     CDS             1947..>3641
                     /locus_tag="PLG14_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706461.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOX49640.1"
                     /translation="MRRITFLSLHVEDSPEAVPLGAASVAAALRGAGFEPVLLEGLAS
                     EPAGALGARILASNPEAVGLSVYSWNRLRAAEVAALLRSRSPGLLLFAGGPEATADPE
                     GLLAAAPLDFLVRGEGETATRLALEAIAAAGPGSSADHDAAAGAEPGCADRPGGARAL
                     SAAAEAALAAIAGIALPGRAAASRRAPPEDLSGLPSPWLAGLVDPRRRGGALWELARG
                     CPFHCAYCYEGKGESGVRRFPRGRVEAELGIFAAAARSGGETLQVFVLDPTFNADRAR
                     ARELLALFGKRGGGIHWKFEARAEFIDRDLARRFADLDCSLQIGLQSAVPEVSGRVGR
                     RLDPADFSRRIGYLNEAGAVFGLDLIYGLPGDDPAGFARSVDFALGLLPNHLDIFPLA
                     VLPGTELADRAPEYGLSHSKEPPYLVASTPGFPPEAMARASRLAAACDLFYSRGRAVS
                     WFLQALRPLKARPSAFLSRFAESLAANGPDGAAGAAGPRGIEALQLAFLEGEYRAKGL
                     ERLLPALRDVVRFNGAWGRALAEGERTELELSYDPDEVLGPAALDLAAFARAARPSP"
BASE COUNT          370 a         1472 c         1273 g          526 t
ORIGIN      
        1 acgcgatgag cgacgcccag tcggccgcga tgtccctggc ggacgaggcc tacgccgggt
       61 cggcgagctt cgagcggctg gaggccgccg tgcgcgaggt cctgggcaag gggctcgtcc
      121 tgcccgtgca ccagggccgg gcggcggagc acatcatctg ctcgagcctc gtggagccgg
      181 gctcggtcgt cccgatgaac taccacttca ccacgacccg cgcccacgtg gagctcgcgg
      241 gcggctccat cgccgagctc atcgaccccg cggccctgga tcccgccgcg gaggagccct
      301 tcaaggggaa catggacgtc tcgaggctcg aggagctcct ctccggcgcg gccggggcgc
      361 gggtgccctt cgtccgcatg gaggcctccg ccaacctcat cgggggccag cccttctccc
      421 tcgcgaacct ccgcgcggtg tcgcgcgccg ccaagagccg gggcgtgccc ctcgtcctgg
      481 acgcgagcct cctcgcggag aacgtcctcc tcatcaagcg ccgcgaggaa ggctgcgggg
      541 gcaagtcgat cgccgagctt ttcctggagt gctgctccct cgccgacctc gtgtacttct
      601 ccgcgaggaa gctgtcctcc tcccgcggcg gcgcgatcgc gacggacgac cccgcgctct
      661 tcgcgaggat gcgcgacctc gtgcccctct tcgagggctt cctcacctac ggcggcatct
      721 cgctccgcga cgtcgagtcg atggcggtcg gcctccgcga gtccctggac gagaccgtga
      781 tcggccagtc gcccgccttc atcgagcacc tggccaggcg cctcgacgcg gccggcgtgc
      841 ccgtcgtcct cccccccgga gccctcggcg tccacgtgga cgcgcgcgcc ttcctgcccg
      901 gcctcagggc ggccgactac ccggcgggcg ccctcgcggc cgccttctac atcgcctccg
      961 gcgtccgggg catggagcgc ggcgcgatat ccgcgggccg cgacgagtcc ggccgtgagg
     1021 tccccgccga catggagctc ctccgcctgg ccttcccgcg ccgcgtcttc accctctcgc
     1081 agctccgcta cgcggaggac cggctcgagt ggctcttcgc gaaccggggc ctcgtcggcg
     1141 gcctgcgttt cgtcgaggag ccctcgaccc tgcgcttctt cacgggaagg ctcgacgcgg
     1201 tcggcgactg gcccgagcgg ctcgccgccg ccttccgggc cgagttcggg gaggaggtct
     1261 aggcccgggg ccggccctcc tccggggtcg gccctccgcc ggggcgcggc aggccgcggc
     1321 ggggctcgcc gaggcgggcc ctgccgcgct atgctggcgg cggtgacagt gtccatgccc
     1381 ccggcctccg ccttcgagcg cgcggccttc tactgcgagg agaacgtcct ccgccttctc
     1441 gcctctcccg ccctcgcggg cctgccggcc tgggcccttc tcgtctccaa cgcggcccgc
     1501 gccgtggcca tgcgcggcca ggccgcgggg cggggcccgg ccggcctcgt cgtctgggac
     1561 taccacgtcc tggccctcgc ggaggccggg ggccgcctct gcgccctcga cctcgacacg
     1621 accctcggct tccccctgga ggcccaggcc tatgtggagg cggccttccc gcgcggcctc
     1681 tccccggagg aagagccccg cttccggctc gtcccctccc gcgactacgt ggagggcctg
     1741 gcctccgacc gctcgcacat gcgccgctcc gacggctcct ggctcgcgcc gcccccgccc
     1801 tgggatccgc ccggcgcggg gaaggctccg aacctcctct cctggatcga tcccgagggc
     1861 gaggggcctg gccaggtcct gggcctcgag gagctccgct ccttcgcccg cgggcgccgt
     1921 ccggccgcgc cggcggggag ccggccatga ggcgcataac cttcctctcc ctccacgtcg
     1981 aggattcccc ggaggccgtc cccctgggcg cggcctcggt cgcggccgcg ctccgcgggg
     2041 ccggcttcga gcccgtcctc ctcgagggcc tcgcctccga gcccgccggg gccctgggcg
     2101 cgcggatcct ggcctccaat cccgaggccg tcggcctctc cgtctactca tggaaccgcc
     2161 tccgcgccgc ggaagtcgcc gccctcctca ggtcccgttc tcccggcctg ctcctcttcg
     2221 ccggaggtcc cgaggcgacg gccgaccccg agggtctcct cgcggcggcc ccgctcgact
     2281 tcctcgtccg cggcgagggc gagacggcca cgcgcctggc cctggaggcg attgccgccg
     2341 cgggcccggg aagctccgcg gaccatgacg ccgcggccgg cgcggaaccg ggctgtgccg
     2401 accgtccagg cggcgcgcgc gccctgtccg ccgcggccga agcggccctt gccgcgatag
     2461 ccggcatagc cctgcccggc cgggccgcgg cctcgcgccg ggctcccccg gaggacctgt
     2521 ccggcctgcc ctcgccctgg ctcgcgggcc tcgtcgatcc gcggcgccgc gggggagcgc
     2581 tctgggagct cgcgcgcggc tgccccttcc attgcgccta ctgctacgag ggcaagggcg
     2641 agtcgggggt caggcgcttc ccgcgagggc gggtcgaggc cgagctcggg atcttcgccg
     2701 ccgcggcccg ctcaggcggc gagacgctgc aggtcttcgt cctggacccg accttcaacg
     2761 ccgacagggc ccgcgcgcgg gagctcctag ccctcttcgg gaagcgtggc ggagggatcc
     2821 actggaagtt cgaggcgcgg gccgagttca tcgaccgaga cctggcgagg cgcttcgcgg
     2881 acctcgactg ctccctgcag atcggcctgc agagcgccgt gcccgaggtg tccggacggg
     2941 tcgggcggcg cctcgacccc gcggacttct cgcgacggat cggctacctc aacgaggcgg
     3001 gcgccgtctt cggcctcgac ctcatctacg gcctaccggg cgacgacccg gcgggcttcg
     3061 cgcgcagcgt agacttcgct ctcggcctcc tgcccaacca cctcgacatc ttcccgctcg
     3121 cggtcctgcc cggcaccgag ctcgccgacc gcgccccgga gtacggtctc tcccattcga
     3181 aggagccgcc ctacctcgtc gcctcgactc ccggcttccc tcccgaggcg atggcccgcg
     3241 cctcgcgcct ggccgcggcc tgcgacctct tctacagccg cggccgcgcc gtgtcctggt
     3301 tcctccaggc cctgcggccg ctcaaggcga ggccttcggc cttcctctcg cgcttcgcgg
     3361 agagcctggc ggcgaatggc ccggacggcg ccgccggggc ggccggccct cgcggcatcg
     3421 aggcgctcca gctggccttc ctggagggcg agtaccgcgc gaaggggctg gagcgcctgc
     3481 tgccggccct gcgcgacgtc gtgcgcttca acggcgcctg gggcagggcc ctggccgagg
     3541 gggagaggac cgagctcgag ctgtcctacg atcccgacga ggtcctgggg ccggcggccc
     3601 tggacctggc cgccttcgcg cgggccgcgc gtccgagtcc g
//
LOCUS       DAOOSJ010000248         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16704_length_3583_cov_3.467687, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000248 DAOOSJ010000000
VERSION     DAOOSJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_3583_cov_3.467687"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLG14_10685"
     CDS             <1..394
                     /locus_tag="PLG14_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49641.1"
                     /translation="ALAPPRPLPEPPFPGDSALEPIRTEAGLFAEGLEMRNCLPRRLG
                     EARAGRAAFYRVLSPFRATLSVERSGADWQGPWVPGELKGPANAALPPEAAALCFERL
                     FATGKKGAWPPGGKPGLRFRQAELPWQD"
     gene            434..1276
                     /locus_tag="PLG14_10690"
     CDS             434..1276
                     /locus_tag="PLG14_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49642.1"
                     /translation="MSTLEERKQVGPMRLDDLVALVAAVAARPAKEAELVEEARARLK
                     EEQEEARRRAIKAVFGDLGGKGPLSWNENALLSPPVEDVRFALGPEGSPDFLARYLPW
                     QGRDPRSGVYFARKNILAALRRNPGVDARAFLGAIAVHELFHCLVERLVGDAAAYAGH
                     ACSPWCGLEEAGANFVARRYAEADPESLATFEALLFRRREAGGLPGYGEYHLLDERIV
                     PAIGYLKSRAAGACVAPADYQKAMAALALAPAALDSHAQAFWAATMTAANSGGVAFYY
                     DMLN"
     gene            complement(1347..3536)
                     /locus_tag="PLG14_10695"
     CDS             complement(1347..3536)
                     /locus_tag="PLG14_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX49643.1"
                     /translation="MSATTETTLSGYEECLRRLATLQGGDAAFFVLAVHSFVEGALRE
                     LVVLDDPADDRFASFVDAFRRKLLGEAKGFIPGLDVLGLLKLQHQLTNDVRHRFASAT
                     KEEARAAAQHLRRFCPLAGIPEGEGLRAVLGLLDAWEDRRSKGDLARELSNLEARLKF
                     EKQGAKEMAARVAALESFQAEAARLKEELRRRERELSELAAVKAAQSGKADALREEAK
                     RLAAELKAAQGKAAEFEDARAYLELLSRLTVLTRSRADFERTIVRPTPEQREVLSQIR
                     LDGDFLVKGSAGTGKTLVLLMAVEKAKGRGGQGELGLPGLEGSAALLTYTNTLVKYDS
                     YVSSILSPEGGADLVQTVDAFLRDRLRALEPGAEIDYDAAKALAERFAPEGVAPRDLA
                     AEAEDFLWANDVDRGRYVDDMIVRQGMKGRLSRSERERCWEACEAMGAEMEKTGRYSK
                     GRSRRRILRALAAEPEDPRLKVVDYVFVDEAQDLTMADLKALKGCARKAVVLAGDADQ
                     GIYLPGFGFKAAGVAIAGRTRVLRTNFRNTLPVHELAERYRAASPGLDAESRPEAYRD
                     GPAPELFVGADRAALLDLLAGRVDLLVRRLGYSPESIAVLVPLGDDVALVRGKLEEAG
                     YASSDIRDRSFEFAGEGAVRVSTLHSSKGLDFPVVLLFLYRAPYFGSAFDEASTELMT
                     RNLVYVAMTRAMDQLAVFALESPSSAAIKDLAASFASVGGADRPSPG"
BASE COUNT          488 a         1291 c         1310 g          494 t
ORIGIN      
        1 ggccctcgct cccccgcggc ccctacccga gccgcccttt ccgggggact cggccctgga
       61 gcccatccgt acggaagccg gcctcttcgc cgagggcctg gagatgagga actgcctgcc
      121 ccgccgccta ggcgaggccc gcgcgggccg cgcggccttt taccgggtcc tctccccctt
      181 ccgcgcgacg ctctccgtcg agcggtcggg cgccgactgg cagggcccct gggtccccgg
      241 cgagctcaag gggccggcca acgcggccct gccgccggag gccgccgccc tctgtttcga
      301 gagactcttc gcgacgggca agaagggggc ttggccgccc ggagggaagc caggcctccg
      361 cttcagacag gccgaactgc cctggcagga ttgagccgcc ctcgcgcggc atgacgcaga
      421 caaggaggtt cccatgtcca cgctggaaga acggaagcag gtcggcccca tgcggctcga
      481 cgacctcgtc gccctcgtcg ccgccgtcgc cgcgcggccc gccaaggagg cggagctcgt
      541 ggaggaggcg cgggccaggc tcaaggagga gcaggaagag gcgcgcaggc gcgccatcaa
      601 ggcggtcttc ggcgacctcg gggggaaggg gcccctctcc tggaacgaga acgcccttct
      661 ttcgccgccc gtggaggacg tccgcttcgc gctcggaccc gagggctccc ccgacttcct
      721 cgcccgctac cttccctggc agggccgcga cccgcggagc ggcgtctact tcgcgcggaa
      781 gaacatcctc gcggccctga ggcggaaccc cggcgtcgac gcgcgggcct tcctgggcgc
      841 catcgccgtc cacgagctct tccactgcct cgtggagcgg ctcgtcggcg acgcggcggc
      901 ctacgcgggc cacgcctgct cgccctggtg cggcctcgag gaggcgggcg cgaacttcgt
      961 ggcccgccgc tacgcggagg ccgatcccga gagcctcgcc accttcgagg cgctcctctt
     1021 ccgcaggcgg gaggcgggag gactcccggg ctacggcgaa taccacctcc tggacgaacg
     1081 gatcgtgccc gccatcggct acctgaagtc gcgggcggcc ggggcctgcg tcgcccccgc
     1141 ggactaccag aaggcgatgg cggccctcgc cctcgcgccg gcagccctgg actcgcacgc
     1201 gcaggccttc tgggccgcga cgatgacggc ggcgaactcg ggcggcgtcg ccttctacta
     1261 cgacatgctc aactaggccg gcggggctcg cccggccggc cgccgcgcct acgagacagg
     1321 cggccgccga ttcggccgca gggccgtcag cccggggacg gccggtccgc gccgccgacc
     1381 gaggcgaagg aggcggcgag atccttgatc gccgcgctgg agggactctc caaggcgaag
     1441 acggccagct ggtccatcgc gcgggtcatc gccacgtaga ccaggttccg ggtcatgagc
     1501 tccgtcgacg cctcgtcgaa ggcggagccg aagtagggcg cgcggtagag gaagaggagg
     1561 acgacaggga agtcgaggcc cttggaggag tgcagggtgg agacgcggac tgcgccttcg
     1621 cccgcgaact cgaagctccg gtcgcggatg tctgagctcg cgtagccggc ctcctcgagc
     1681 ttcccgcgga ccagggccac gtcgtcgccc aggggcacga gcacggcgat gctctcgggg
     1741 gagtagccca ggcgccgcac gaggaggtcg acgcggcccg cgaggaggtc gaggagcgcc
     1801 gcccggtctg ctccgacgaa gagctcggga gcggggccgt cgcgataggc ctcgggccgg
     1861 ctctccgcgt ccaggccggg agaggccgcc cggtagcgct cggcgagctc gtggacgggc
     1921 aaggtgttgc ggaagttcgt gcgcaggacg cgggtcctgc ccgctatcgc gacgccggcg
     1981 gccttgaagc cgaagccggg gaggtagatg ccctggtcgg cgtcgccggc gaggacgaca
     2041 gccttcctgg cgcagccctt gagggccttc aggtcggcca tcgtcaaatc ctgggcctcg
     2101 tcgacaaaga cgtagtcgac gaccttgagc cgcggatcct cgggctcggc cgcgagggcg
     2161 cggaggatcc ggcgccggga gcggcccttc gagtagcggc ccgtcttctc catctcggcg
     2221 cccatcgcct cgcaggcctc ccagcagcgc tcccgctcgg agcgggaaag ccggcccttc
     2281 atgccctgcc gcacgatcat gtcgtccacg tagcgccccc ggtccacgtc gttggcccaa
     2341 agaaagtcct ccgcctccgc ggcgagatcg cgcggcgcga cgccttcggg ggcgaagcgc
     2401 tccgccaggg ccttcgccgc gtcgtagtcg atctcggcgc ccggctccag ggcgcggagc
     2461 cggtcgcgga gaaaggcgtc gacggtctga accaggtccg ccccgccctc gggcgagagg
     2521 atcgaggaca cgtagctgtc atacttcacg agggtgttcg tgtaggtgag gagggccgcg
     2581 gagccctcga ggccgggaag gccgagctcg ccctgccctc cccggccctt cgccttctcc
     2641 accgccatga ggaggacgag cgtcttgccc gtgccggccg agcctttgac gaggaagtcc
     2701 ccgtccagcc tgatctggga gaggacctcg cgctgctccg gcgtggggcg gacgatggtg
     2761 cgctcgaagt cggcgcggct ccgggtgagg acggtgagcc gcgacaggag ctcgaggtag
     2821 gcgcgggcgt cctcgaactc ggcggccttt ccctgcgcgg ccttgagctc ggcggcgagc
     2881 cgcttggcct cctcccgcag ggcgtccgcc ttcccggact gggccgcctt gacggcggcg
     2941 agctcggaga gctcccgctc gcggcggcgg agctcctcct tgagccgcgc cgcctccgcc
     3001 tggaaggact ccagggccgc gacccgtgcg gccatctcct tcgcgccctg cttctcgaac
     3061 ttgagccgcg cctccaggtt ggagagctcc ctcgccaggt cgcctttgct cctcctgtcc
     3121 tcccaggcgt cgaggaggcc caggaccgcg cggagcccct cgccctccgg gatgccggcg
     3181 aggggacaga agcgccgcaa gtgctgggcc gcggcgcggg cctcctcctt ggtcgcggag
     3241 gcgaagcggt gccgcacatc gttcgtgagc tggtgctgga gcttgaggag gccgaggacg
     3301 tcgaggccgg ggatgaagcc cttcgcctct cccagcagct tccgccggaa ggcgtcgacg
     3361 aagctcgcga agcggtcgtc cgccggatcg tccaggacga cgagctcgcg gagggcgccc
     3421 tcgacgaagg agtggacggc gaggacgaag aaggccgcgt cgccgccctg gagggtggcc
     3481 aggcgcctga ggcattcctc gtagcctgaa agggtcgttt cggtcgtggc gctcatcggg
     3541 gggcttcctt gccggtcggt ttcgccgccc actatagccc cgc
//
LOCUS       DAOOSJ010000249         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16761_length_3576_cov_5.572849, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000249 DAOOSJ010000000
VERSION     DAOOSJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16761_length_3576_cov_5.572849"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLG14_10700"
     CDS             complement(<1..1640)
                     /locus_tag="PLG14_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX49644.1"
                     /translation="MRPKTVLSVAAASAIGLALAALGQHVLALGWLLCATMAILSFAD
                     DGRTSTKTAVSSSLGLVGVFVFASRYGIPAAEGGSVLGEFERLAEAVHLDIFVFLAGL
                     YLVVNTFAYSGFIGDLAWKIVKRAEGRLGRIMVAIMLLTCVLSGIFDGATITTIMGIV
                     TLTILLSSGMSARHIVEILLLLVVATNIGGVWFVLGEPTNILAAAKLGLSPFFFVRYA
                     SLFALPAAGLCAFAAWRIVRRYPKIRSDRPEMEVLLEGISLRRAHSGAGTLADTLESI
                     GTVEVRSLAEMARIVEEEGLPDFEAALKAGIPQKKVHAALSINLNSEELARGLIDFYH
                     YRAEGDPVAEIVLGDLLHHVRDEYKARTKSRRLVLASGAVLIALLVLHAFLPGMPTWA
                     STVLAGAIAIAAVQPNARRHILAQTRHNMTEAFFLVAIFVTISELNFTGAFETLGKSI
                     LGLGGAGATGAGILAGSALFSAVADNVAVIDVLTNLIRRHEDWSFFALAGIVGTALGG
                     FASPIASVQAVIMATIVRRVAKVGFGKWVGITALWFLAL"
     gene            1906..2901
                     /locus_tag="PLG14_10705"
     CDS             1906..2901
                     /locus_tag="PLG14_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOX49645.1"
                     /translation="MKAMVIREFGPPSVLRLEEPQRPRPGKGEVLVRVAATSVNPIDA
                     KIRAKGLGLAADFPATLHCDLSGVVEELGSGVSRLKPGERVFGLGGGVKGRGGALAEF
                     VAVDERLLAPLPSGLGLVEAAALPVVGLTAQEGLARAGLSHGERLLVQGGSGGVGGCV
                     MELARGLGAEVVATASARHLGLLGEAGAREAFDYKRPDLEEALLAASGGEGYDLVFDT
                     AGGPSLDLAFRLARPGGRVVAIAARSTHDLSPLHGKGLSLHVVFTLLPLLSGRGIEEL
                     HARLAGIAAAAAAGGLRPRLDPRRFPLAQAAAAHELFESGGAEGKILVVVDGSLA"
     gene            complement(3059..>3576)
                     /locus_tag="PLG14_10710"
     CDS             complement(3059..>3576)
                     /locus_tag="PLG14_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49646.1"
                     /translation="SCVLALVTGPAGFAEARTESLAPRWSAESAIGLDGGAAALFMAP
                     RDGRLSLGLRLGLGLELADFKWNDPSASLALRYRLLGAGRFEGWIGAEAGAIALDTGY
                     DRFALPFAGATAGCAFRLAGGLSLFAEAGCRYGRRDRRYESSLAFLDASYAETVFALP
                     AILRLGLSLSF"
BASE COUNT          516 a         1262 c         1335 g          463 t
ORIGIN      
        1 agggccagga accagagggc cgtgatgccc acccatttcc cgaagcccac cttcgcgacc
       61 cggcggacga tcgtggccat gatgacggcc tggacggagg cgatgggcga ggcgaagccg
      121 ccgagcgccg tcccgacgat gcccgcgagg gcgaagaagg accagtcctc gtggcggcgg
      181 atgaggttgg tgaggacgtc gatgaccgcc acgttgtccg ccaccgcgga gaagagggcg
      241 ctgccggcca ggatccccgc tcccgtggct cccgcgcccc cgaggcccag gatgctcttc
      301 ccgagcgtct cgaaggcgcc cgtgaagttg agctcggaga tcgtgacgaa gatggcgacg
      361 aggaagaagg cctcggtcat gttgtgccgg gtctgggcca ggatgtgcct tctcgcgttc
      421 ggctgcacgg ccgcgatggc gatggccccc gccaggacgg tcgacgccca ggtcggcatg
      481 ccgggcagga aggcgtggag gaccaggagg gctatgagga cggcgccgct cgccaggacg
      541 aggcgcctgc tcttggtcct cgccttgtac tcgtcgcgga cgtggtggag gaggtcgccg
      601 aggacgatct ccgccacggg atcgccctcg gcgcggtagt ggtagaagtc gatgaggccc
      661 cgggccagct cctcggagtt caggttgatg gagagggccg cgtggacctt cttctgcggg
      721 atgccagcct tgagcgccgc ctcgaagtcc ggcaggccct cctcttccac gatgcgcgcc
      781 atctccgcga gggagcggac ctccaccgtg ccgatgctct cgagcgtgtc ggccagcgtg
      841 cccgcgccgg aatgggcgcg gcggagcgag atgccctcca ggaggacctc catctccggc
      901 ctgtcgctcc ggatcttggg atagcgcctg acgatccgcc acgcggcgaa ggcgcagagg
      961 cccgcggccg gcagggcgaa gagggaggcg taccggacga agaagaaggg cgagaggccg
     1021 agcttcgcgg ccgcgaggat gttcgtcggc tccccgagga cgaaccagac gcctccgatg
     1081 ttcgtcgcta cgacgaggag gaggaggatc tcgacgatgt gccgggcgct catcccgctc
     1141 gagaggagga tggtgagcgt gacgatgccc atgatcgtgg tgatcgtcgc gccgtcgaag
     1201 atccccgaga ggacgcaggt gaggagcatg atggcgacca tgatccggcc cagccggccc
     1261 tcggcgcgct tcacgatctt ccaggccagg tcgccgatga agcccgagta ggcgaaggtg
     1321 ttgacgacga ggtagaggcc cgccaggaag acgaagatgt ccaggtggac ggcctcggcc
     1381 aggcgctcga actccccgag gacgctgccg ccctcggcgg cggggattcc gtagcggctc
     1441 gcgaagacga agacgccgac gaggcccagg gagctcgaga ccgcggtctt ggtgctcgtg
     1501 cggccgtcgt ccgcgaagga gaggatggcc atcgtcgcgc agaggagcca gcccagggcc
     1561 aggacgtgct ggcccaaggc ggcgagggct aggccgatcg cggaggccgc ggccacggac
     1621 aggacggtct tgggtctcat ggcggaatcc tccagggcgc cgggttcccg ccaaaatacg
     1681 ggctagccgc tccctcgtca agccgggctg gcggccgccc gcacgtcccg cgataatcga
     1741 aggagcgcgc tgagcgtcgc ccagcctcgc cgcgcatagg ccgccgccgc tcggcgcctc
     1801 gtcgaaccgg gcggcgcttc gagccggctc gtcgagccgg cgcgtcgaac cgtcgcgtca
     1861 agcaggcgcg tcagccgccg cccgagccag cccaaggagg catgcatgaa ggccatggtc
     1921 atccgcgaat tcggtccgcc ctcggtcttg aggctcgagg agccgcagcg gccccggccc
     1981 gggaagggcg aggtcctcgt ccgggtggcg gcgacctcgg tgaaccccat cgacgcgaag
     2041 atccgcgcga agggcctggg gctggcggcc gacttccccg cgacgctcca ctgcgacctc
     2101 tcgggcgtcg tcgaggaact cggctccggc gtctcgcgcc tcaagccggg cgagcgggtc
     2161 ttcggtctgg gcggcggtgt caagggcagg ggaggggccc tggccgagtt cgtcgcggtc
     2221 gacgagcgcc tcctcgcgcc gctgccttcc ggcctgggcc tggtcgaggc ggccgccctt
     2281 cccgtcgtcg gcctcaccgc ccaggagggc ctcgcgcggg ccggcttatc ccacggggag
     2341 cggctcctcg tccagggcgg ctcgggcggc gtgggcggct gtgtcatgga actcgcccgc
     2401 ggcctcggcg cggaagtcgt cgcgaccgcc tcggcccgcc acctcggcct cctgggcgag
     2461 gccggcgccc gcgaggcctt cgactacaag cggcccgacc tcgaggaggc cctccttgcc
     2521 gcctcgggcg gcgagggcta cgacctcgtc ttcgacacgg ccggagggcc ttccctcgac
     2581 ctggccttcc gcctggcgcg gcccggcggc cgcgtcgtcg cgatagcggc ccgctcgacc
     2641 cacgaccttt cgcctctcca cgggaagggc ctttccctcc atgtcgtctt caccctcctc
     2701 cccctcctct cggggcgggg gatcgaggag ctccacgccc gcctggccgg gatagcggcg
     2761 gccgcggcgg ccggcgggct caggccgcgc ctcgacccgc ggcgcttccc cctcgcgcag
     2821 gcggccgccg cgcacgagct tttcgagtcg ggcggggccg agggcaagat ccttgtcgtc
     2881 gtcgacggga gcctcgccta gggggggcct cgcggcgtag gggccccgcg gcggacgggc
     2941 cccagacgaa ggccggccct gcccgagggc cggcgccccc tgcgggcggc ccggccaagc
     3001 gccggctccc cctacgggcg gcccggccaa gggccggctc ctctgctgcc aggccccgct
     3061 agaagctcag cgaaaggccg agcctcagga tggcgggcag ggcgaagacc gtctccgcgt
     3121 agctcgcgtc caggaaggcg agcgagcttt cgtatcgccg gtcgcggcgc ccgtagcggc
     3181 agcccgcctc cgcgaagaga gagagcccgc ctgcgaggcg gaaggcgcag cccgccgtcg
     3241 ccccggcgaa gggcagggca aagcggtcgt agcccgtgtc gagggcgatc gcgcccgcct
     3301 cggcgccgat ccatccctcg aagcgccccg cgcccaggag ccgatagcgg agggcgaggc
     3361 tggccgaggg gtcgttccac ttgaaatccg cgagttcgag gccgagcccc agcctgaggc
     3421 ccagggacag gcggccgtcc cggggcgcca tgaagagcgc ggccgcgccg ccgtccaggc
     3481 ctatcgcgct ttccgccgac catcgcggcg ccagggactc agtccgcgcc tcggcgaagc
     3541 ccgccggccc cgtgacgagg gcaaggacgc aggaga
//
LOCUS       DAOOSJ010000250         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16771_length_3575_cov_5.792045, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000250 DAOOSJ010000000
VERSION     DAOOSJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16771_length_3575_cov_5.792045"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1346
                     /locus_tag="PLG14_10715"
     CDS             348..1346
                     /locus_tag="PLG14_10715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOX49647.1"
                     /translation="MDAKGMGREELLALLKGSELLSGLLDEDLDYVAESSELFALPSG
                     GVLFGPGERAKRFYLVRSGEIGAFRAEDPERQVARFVQGDAVGDFDFAAGAVPDAECR
                     ALCPAELVAFPRRGLGMQDLARERPDASARILLRCLSMISARLRSTQRLISENAPWVR
                     ELKRQLYTDASTGLHSRAFLDEELPRLIEAPTALVLLKPDRFKELNDAHGHGAGDEAM
                     SLIAGILVRETEALGRGWALRLRSNETALVVPRSGREEAAEIARRLAAAVAALELPCA
                     AGTGFAFTSSLALAVWPEDGANWRRLVEGAYGLLTKAWRDGGARAYRLKPREEARP"
     gene            1343..2323
                     /locus_tag="PLG14_10720"
     CDS             1343..2323
                     /locus_tag="PLG14_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOX49648.1"
                     /translation="MSHVTDFLSGLPLCEGMSGLEVAAVAAFLEARRFEAGEVVFREG
                     ETGNELFIVRSGRIASYAALPDGSRRQVYEFGPGLLFGEMAIIEGEPRTATCSALEPS
                     ELFVLDGLDFYRLVWDHPVIGVKLLAAMGRVMSAWLDEASGFLGGLVRWGEAARKRAV
                     IDDLSGLFNRRFLDDTMRTRFASGSGSKPRASLLMLDLDRFRDLNATFGPAAGDAAIA
                     SAGAAFGTLVRGEEVAARLSGDEFAFFLPGAGPERARELAEELRAAAEGLFLEFRAGP
                     GSKPARASLTASVGFASAPEDAATAEELSAAADKALYRAKEGGRNRVERA"
     gene            complement(2424..3032)
                     /locus_tag="PLG14_10725"
     CDS             complement(2424..3032)
                     /locus_tag="PLG14_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49649.1"
                     /translation="MRVKLHVALAAMALAALALASLPAAAEESSPQSPVFSARAGLGT
                     DINLGVAVGGGFGWLKEFAGLPPVDFGIDFYYYDGTEISEEQVGPSLNTYEDMTTLMV
                     FAVSADFLHGYEAGARGFYYLTGLGAAAVSVEWVGYSADDPSYNDSRDYASAGLLVKL
                     GVAYAFGKGLELRLVAPILVFEGPQDSLGFAPMLNLAAALRF"
     gene            3113..>3575
                     /locus_tag="PLG14_10730"
     CDS             3113..>3575
                     /locus_tag="PLG14_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49650.1"
                     /translation="MRKSRYAAFCLLLLLPTRFLFAAPGEGTPLPLSPRLAPLAGTDA
                     SSGAMAKAPLDPATPDAVSAATGQDQGGRFLGFLSKTAFHKFAGWTSGGLLLAAGVVG
                     GIRAYDLMNSGHEYRDTLGITEEDQIGAQCTGEISSLWGDSAGQALRWTHVG"
BASE COUNT          466 a         1326 c         1287 g          496 t
ORIGIN      
        1 gcccgggggc gccgcggccg gcgcaggagg atagcgcggc cgggatggac agcgtgaggg
       61 caagggcgag ggcgaggcgc ggccaggcta tcgcggcggc tcggtgcggc ttcatgcggg
      121 gctccttgga gacaggagta tagcccggga ctcaggcccg cgagctagtc aattcggggg
      181 attccggccg cctgaggcag ggtgtccgcg cgcccggcgt ccgcgctccc tgctccggcg
      241 ttcgcggagc ctcgcggcgg gccatggggc cggatccgcc ggcgcggccg cccccgcggc
      301 gggcccgccg ttgacccgga ggcgctcccg ggcctatagt cgggcccatg gacgcgaaag
      361 gcatgggccg ggaggagctg ctcgccctcc tcaagggctc ggagctcctc tccgggctcc
      421 tcgacgagga ccttgactac gtcgccgaga gctccgagct cttcgccctc ccctcgggcg
      481 gggtcctctt cgggcccggc gagcgggcca agcgcttcta tctcgtgcgc tccggggaga
      541 tcggcgcctt ccgggccgag gacccggagc ggcaggtcgc gcgcttcgtg cagggcgacg
      601 cggtgggcga cttcgacttc gccgccggcg ccgtccccga cgcggagtgc cgggccctct
      661 gccccgcgga gctcgtcgcc ttcccgcgcc ggggcctcgg gatgcaggac ctggcccggg
      721 agcggcccga cgcctcggcc cgcatcctcc tccgctgcct ctcgatgatc tccgcccggc
      781 tccgctcgac ccagcgcctc atctccgaga acgcgccctg ggtgcgcgag ctcaagcgcc
      841 agctctacac ggacgcctcc acgggcctcc actcgcgggc cttcctggac gaggagctgc
      901 cccgcctcat cgaggcgccg acggccctcg tcctgctcaa gcccgaccgc ttcaaggagc
      961 tcaacgacgc ccacggccac ggcgcggggg acgaggcgat gtcgctcatc gcgggaatcc
     1021 tggtgcgcga gacggaggcc ctggggcggg gctgggccct ccgcctccgc tccaacgaga
     1081 ccgccctggt cgtgccccgc tccgggcggg aggaggccgc ggagatcgcg cgccgcctcg
     1141 cggcggccgt cgccgccctc gagctcccct gcgcggccgg cacgggcttc gccttcacct
     1201 ccagcctcgc cctcgccgtc tggccggagg acggggcgaa ctggcgccgc ctcgtggagg
     1261 gcgcctacgg cctactcacg aaggcctgga gggacggcgg ggcccgcgcc taccgcctga
     1321 agccgcgcga ggaggcccgc ccgtgagcca cgtcacggac ttcctctcgg ggctgcccct
     1381 ctgcgagggc atgagcggcc tcgaggtggc cgcggtcgcc gccttcctgg aggcgcgccg
     1441 cttcgaggcg ggcgaggtgg tcttccgcga gggcgagacg gggaacgagc tcttcatcgt
     1501 ccgctcgggg cgcatcgcct cctacgcggc cctgcccgac gggagccgcc gccaggtcta
     1561 cgagttcggc cccggcctcc tcttcggcga gatggcgatc atcgagggcg agccgcggac
     1621 cgccacctgc tcggccctgg agccctccga gctcttcgtc ctcgacggcc tcgacttcta
     1681 ccgcctggtc tgggaccatc ccgtgatcgg cgtgaagctc ctcgcggcga tgggccgcgt
     1741 gatgtcggcc tggctggacg aggcctcggg cttcctgggc ggcctcgtgc gctggggcga
     1801 ggccgcgagg aagcgcgccg tcatcgacga cctctccggc ctcttcaacc ggcgctttct
     1861 ggacgacacg atgcggacgc gcttcgcgag cggctcgggc tccaagccgc gggcctccct
     1921 cctcatgctc gacctggacc gcttccggga cctgaacgcc accttcggcc ccgcggccgg
     1981 cgacgcggcg atcgcgagcg ccggcgcggc cttcggcacc ctggtgcggg gcgaggaggt
     2041 ggccgcgcgg ctctccggcg acgagttcgc cttcttcctt cccggcgccg gccccgagcg
     2101 ggcgcgcgag ctcgccgagg agctcagggc cgcggcggag gggctcttcc tcgagttccg
     2161 cgcggggccc ggctcgaagc cggcgcgggc ttcactcacg gcgagcgtcg gcttcgcctc
     2221 cgcgccggag gacgcggcga cggcggagga gctttcggcg gccgccgaca aggcccttta
     2281 ccgcgcgaag gaaggcggaa ggaaccgcgt ggagcgggcc tgaggcctgc ccgcgacgcg
     2341 gtttgcggcc ggcctgcgac tgagtccgcg ccgcgcccgc ggaacggccg ggcgcgaccg
     2401 acagcgatag tcccgcggcg cggctagaag cggagggccg cggccaggtt gagcatcggc
     2461 gcgaagccaa ggctgtcctg ggggccttcg aagacgagga tgggcgcgac gaggcgcagc
     2521 tcgaggccct tgccgaaggc gtaggcgacg ccgagcttca cgaggaggcc ggcgctcgcg
     2581 tagtcccgcg agtcgttgta gctcgggtcg tcggcgctgt agccgaccca ctcgacgctc
     2641 accgcggccg cgcccaggcc cgtaaggtag tagaagcccc tcgcgcccgc ctcgtagccg
     2701 tggaggaagt cggcgctcac cgcaaagacc atcagcgtgg tcatgtcttc gtaggtgttg
     2761 agcgaagggc ccacctgctc ctctgagatc tcggtcccgt cgtagtagta gaagtcgatc
     2821 ccgaaatcga ccgggggaag gcccgcgaat tccttgagcc agccgaagcc gccgccgaca
     2881 gccacgccga ggttgatgtc ggtgcccagg cccgccctcg cggagaagac gggactctgc
     2941 ggcgagcttt cctccgccgc ggccgggagg gaagccagcg ccaaggcggc aagcgccatg
     3001 gcagccagcg cgacatgaag cttgacacgc atgacaacct ccgcgcggga aagctgatat
     3061 aaatgctaag gcaagtccgg cgccggggca aggcttcgac tatgctataa gcatgcgcaa
     3121 atccaggtac gcagccttct gccttctcct ccttctcccg acccggttcc tcttcgccgc
     3181 ccctggcgag ggcacgccgc tccccctctc gccgcgcctg gcgccgctcg cgggaaccga
     3241 cgcctcttcc ggggccatgg ctaaggctcc cctcgatccg gccacgccag acgcggtctc
     3301 cgccgcgacg gggcaggacc agggcggccg cttcctcgga tttctcagca agacggcctt
     3361 ccacaagttc gccggctgga cctccggcgg cctcctcctc gccgcgggag tcgtcggcgg
     3421 catccgcgcc tacgacctca tgaactcggg ccacgagtac cgcgacacgc tggggatcac
     3481 cgaggaggac cagatcggcg ctcaatgcac gggggagatc tcgagcctct ggggcgattc
     3541 agcgggccag gccctgcgct ggacccacgt cggcc
//
LOCUS       DAOOSJ010000251         3565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16834_length_3565_cov_5.293732, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000251 DAOOSJ010000000
VERSION     DAOOSJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16834_length_3565_cov_5.293732"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLG14_10735"
     CDS             <1..1363
                     /locus_tag="PLG14_10735"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOX49651.1"
                     /translation="APPEIKSALAGAESVQSAFRYVAKSVLPSVVEVKVVETKKGGPQ
                     AAPSDQFPWRFFFGQPDDQQEEAPKDYQEQGLGSGIIVRRDGGAVYVLTNNHVAGEAS
                     DITVVLSDDREFKATLVGKDERKDLALVKFETSAKDIVVATLGDSSALEVGDWAIAIG
                     SPFGFVSSVTIGTVSALGRSSGPDGNISDFIQTDAAINRGNSGGALVNIRGEVVGINT
                     WIASPTGGSIGLGFAIPINNAKKAIDDFITRGKVEYGWLGVSLRGLDKASSEELGVGD
                     RKGAFVAHVFSGSPADKAGFLPGDYIVEADGAAVRGQDQVVRTVGDLPVGRKARFAAI
                     RDGKRIELVVTIEARKPSVASNDGNLFPGLDVISLKSEDIDKSKLPKDAKGILVTNVF
                     PKSPAATVGLKPGDIVTEVNEKPVAGLGDFFRLLNDPKERKIAFTVNRDGQTVSTLAY
                     VRK"
     gene            1538..2092
                     /locus_tag="PLG14_10740"
     CDS             1538..2092
                     /locus_tag="PLG14_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOX49652.1"
                     /translation="MAFRIHQEGFVPDVIYVSLRGGAYIGNVISEYFKIVRKDSRPIF
                     YAAVVARSYADINQREQVRVDGWTYSPEYLRHGDKVLLVDDIYDSGRTVNHLTEIILE
                     KGLPRRDVKVAVHDYKVCEYRDDRLPIVPDYWCRRHVITRPEDEVWIHYMSHELVGLS
                     EDELRTHYYPEDPELQEILRVLKK"
     gene            2097..>3565
                     /locus_tag="PLG14_10745"
     CDS             2097..>3565
                     /locus_tag="PLG14_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49653.1"
                     /translation="MASRLTAPALLVLLGLAAGAGRAAAEGAASSSFPLYQPEAAPAV
                     SRSDFGLLPPSEPGAAAYFRFGPEAPWTLFDRPLRLGAAPREDRAYGLEVAVEGPGGR
                     RETAYRFRIDKLPPAPPRASPSQGVLREAVAVSLSAEAGSSLRCALLGPGSAELVFEP
                     YDPSRRPVLRPPASGTETVVLLAYAVDEAGNGGEPSRFVYRLAASGLGVAEPPAQAPR
                     LPPRADPLPELAATLVAQEAGRSVVSVEAPESAAVLAAVNPRDPLADAAAYAPLQRSG
                     GKALLVLECPYGYSGEARVHLAVQDSSGLRARSEALFVPLAHRPEAARVPAPPEGPSL
                     IAGPPGSGALVAFPAADGELYVSMAGSPFRPYSQPLSLPPGEGSVELRWYGVSAGGKS
                     ETASALLKRPAAIPDIGVLGAPSGAATREAVRLRPSGPGTLRYELSSDGSVPPEPGPA
                     SPLLGQGLLVECPEGEERSYAFRFRPFAGEAPDAPAGEGG"
BASE COUNT          522 a         1380 c         1160 g          503 t
ORIGIN      
        1 cgcccctccc gagatcaagt ccgccctggc cggggccgag agcgtccaga gcgccttccg
       61 ctacgtcgcc aagagcgtcc tcccctcggt ggtcgaggtg aaggtcgtgg agacgaagaa
      121 gggcgggccc caggccgcgc cctccgacca gttcccctgg cgcttcttct tcggccagcc
      181 cgacgaccag caggaagagg ctcccaagga ctaccaggag cagggcctgg gctcggggat
      241 catcgtgcgg cgcgacggcg gcgcggtcta cgtcctcacc aacaaccatg tcgcgggcga
      301 ggcctccgac atcaccgtcg tcctctcgga cgaccgcgag ttcaaggcga ctctcgtcgg
      361 caaggacgag aggaaggacc tggccctcgt gaagttcgag acctccgcga aggacatcgt
      421 cgtggcgacc ttgggcgact cctcggccct ggaggtcggc gactgggcca tagccatcgg
      481 cagccccttc ggcttcgtct cctcggtgac catcggcacc gtgagcgccc tgggccgctc
      541 ctcggggccc gacggcaaca tctcggactt catccagacg gacgcggcca tcaaccgcgg
      601 caactccgga ggcgccctgg tcaacatccg cggcgaggtc gtcggcatca acacctggat
      661 cgcctcgccc acgggcggca gcatcggcct cggcttcgcc atcccgatca acaacgccaa
      721 gaaggccatc gacgacttca tcacccgggg caaggtcgag tacggctggc tcggcgtctc
      781 cctgcgcggc ctcgacaagg ccagctccga ggagctcggc gtcggggacc ggaagggggc
      841 cttcgtcgcc cacgtcttct ccggaagccc cgcggacaag gccggcttcc tccccggcga
      901 ctacatcgtc gaggcggacg gcgcggcggt gaggggccag gaccaggtcg tgcggaccgt
      961 cggggacctg cccgtcgggc gcaaggcccg cttcgccgcg atccgcgacg ggaagcggat
     1021 cgagctcgtg gtcaccatag aggcgaggaa gccctcggtg gcctccaacg acgggaacct
     1081 cttcccgggc ctcgacgtca tctccctcaa gtccgaggac atcgacaagt cgaagctgcc
     1141 caaggacgcg aagggcatcc tggtcacgaa cgtcttcccc aagtccccgg cggccaccgt
     1201 gggcctcaag cccggagaca tagtcacgga ggtgaacgag aagcccgtcg ccggcctggg
     1261 cgatttcttc aggctgctca acgacccgaa ggagaggaag atcgccttca ccgtcaaccg
     1321 cgacggccag acggtctcca cgcttgcata cgtgaggaag tgacgctata gtctttccat
     1381 gggcggccgc ggggaacagc ctttcccgcg gccgccttcg tcgagcgacc gcgcgccccg
     1441 agaggggccg accgcggcgc cggaaggcga ggtcaccgtg tccaccgttc gcaaggaatt
     1501 cgtgccgtac aacgtcgtcc gcaacaacgc cctcaagatg gccttcagga tccaccagga
     1561 gggcttcgtc cccgacgtca tctacgtctc cctgcggggc ggcgcgtaca tcgggaacgt
     1621 catcagcgag tacttcaaga tcgtcaggaa ggactcgcgg cccatcttct acgccgccgt
     1681 cgtcgcgcgg tcctacgcgg acatcaacca gcgcgagcag gtcagggtgg acggctggac
     1741 ctacagcccg gagtacctgc gccacggcga caaggtcctc ctcgtcgacg acatctacga
     1801 ttccggccgc accgtcaacc acctcacgga gatcatcctg gagaagggcc tgccgcgccg
     1861 cgacgtcaag gtcgcggtgc acgactacaa ggtctgcgag taccgggacg accgccttcc
     1921 catagtcccc gactactggt gcaggcgcca cgtcatcacg cggcccgagg acgaggtctg
     1981 gatccactac atgagccacg agctcgtggg cctctccgag gacgagctca ggacccacta
     2041 ctatcccgag gaccccgagc tccaggaaat tctccgcgtc ctcaagaagt gagcccatgg
     2101 cgagccgcct gaccgcgcct gccctcctcg tactcctcgg cctcgcggcc ggagcggggc
     2161 gggcggcggc cgaaggcgcg gcctcctcct ccttccccct ctaccagccc gaggccgccc
     2221 ccgcggtgtc gaggtcggac ttcggcctcc tcccgccctc cgagccgggc gcggcggcct
     2281 atttccgctt cggccccgag gcgccctgga ccctcttcga ccggccgctc aggctgggcg
     2341 ccgcgccccg cgaggacagg gcctacggcc tcgaggtggc ggtggagggg ccgggcggcc
     2401 ggcgcgagac cgcctatcgc ttccggatcg acaagctgcc gcccgctccc cccagggcct
     2461 cgccctccca gggcgtcctg cgcgaggccg tcgcggtctc cctctccgcc gaggccggct
     2521 cctccctgcg ctgcgccctc ctgggcccgg gctctgccga gctcgtcttc gagccctacg
     2581 acccctcccg ccgccccgtc ctgaggccgc cagcctcggg cacggagacc gtcgtcctcc
     2641 tcgcctacgc ggtggacgaa gcggggaacg gaggcgagcc ctccaggttc gtctatcgcc
     2701 tcgcggcctc cggcctcgga gtggccgagc cgccggccca ggccccgagg ctcccgccca
     2761 gggccgatcc cctccccgag cttgccgcga ccctcgtggc gcaggaggcc gggcgcagcg
     2821 tcgtctcagt ggaggctccc gaatcggcgg ccgtcctggc cgcggtgaat ccccgcgatc
     2881 ccctcgccga cgccgcggcc tacgcccccc tccagcgctc gggcgggaag gccctcctcg
     2941 tcctcgagtg cccctacggc tattcgggcg aggcgagggt ccacctggcg gtccaggact
     3001 cctcgggcct ccgcgcgagg agcgaggccc ttttcgtccc cctcgcgcac cggcccgagg
     3061 cggcgcgggt cccggccccg cccgagggtc cgagcctcat cgctggcccg cccggctcgg
     3121 gcgccctcgt cgcctttccc gccgcagacg gcgagctcta cgtctcgatg gccggctcgc
     3181 ccttccggcc ctacagccaa cccctctccc tgcctccggg ggagggctcc gtcgagctgc
     3241 gctggtacgg cgtctcggcc ggcggcaaga gcgagaccgc cagcgccctc ttgaagcgcc
     3301 ccgccgcgat acccgacata ggcgtcctcg gcgcgccctc gggagccgcc acccgcgagg
     3361 ccgtccgcct ccggccctcg gggcccggaa ccctgcgcta cgagctttcc tcggacggct
     3421 ccgtcccgcc cgagccgggc cccgcctcgc cgctgctcgg ccaaggcctc ctcgtggaat
     3481 gccccgaggg cgaggagcgg agctacgcct tccgcttcag gcccttcgcc ggggaggctc
     3541 cggacgcccc cgcgggagag ggcgg
//
LOCUS       DAOOSJ010000252         3557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16887_length_3557_cov_4.640777, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000252 DAOOSJ010000000
VERSION     DAOOSJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16887_length_3557_cov_4.640777"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..865)
                     /locus_tag="PLG14_10750"
     CDS             complement(209..865)
                     /locus_tag="PLG14_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOX49654.1"
                     /translation="MDDDILTIEEVARYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     SEIERWVNERLSSNKPASVFSVVQIENILSPDRILFLEHSKKRDALVALSENLATAPQ
                     IKNRQELVSEIIRREELMSTAIGRGIAIPHVRLSSVTDLVVSVGLSKCDIIDFQSLDD
                     VPVRLLFMIAAAYNQHAYYLQTLSFFSSKLKSTELRTSLLAAKTPAEVYELLIKQDDQ
                     "
     gene            complement(977..1588)
                     /locus_tag="PLG14_10755"
     CDS             complement(977..1588)
                     /locus_tag="PLG14_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOX49655.1"
                     /translation="MRIESVRKGASGSATFAAGGSSFFVAFRYLAALGLDPEALVPGL
                     ELEESGAEALSLAAEATEAERRALALLARAEQCRLGLEMKLAKRELGRRAVALALDRL
                     AAEGLLDDRRYAEAWLRTRLKKGEGPSRLLLALRARGIGEEAAKAALAEVLGPEERDS
                     ALAKAARRELGRAEGDRDRAAQALRAQGFKAAEIRACLEALGE"
     gene            complement(1599..1991)
                     /gene="rpsI"
                     /locus_tag="PLG14_10760"
     CDS             complement(1599..1991)
                     /gene="rpsI"
                     /locus_tag="PLG14_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOX49656.1"
                     /translation="MIRNLGIGTGRRKCAVARVFLREGTGKVVVNGQELDKYFKVGEL
                     VTIVRQPLMVTASENKYDILIKVLGGGPNGQAGACRHGVARALTQVDPANRTALKANG
                     FLTRDPRMVERKKYGQAGARRRFQFSKR"
     gene            complement(2005..2433)
                     /gene="rplM"
                     /locus_tag="PLG14_10765"
     CDS             complement(2005..2433)
                     /gene="rplM"
                     /locus_tag="PLG14_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOX49657.1"
                     /translation="MKTVFVTPDEATRSWYVIDAEGKPLGRVAAKVASMLRGKNKPTY
                     EPSQETGDFVVVINADKVAISGRKRQNKMYYHHTGFPGGLKDFTFKQLIERHPTEPLE
                     RAVYGMLPKGPLGRKLYKNVKFYAGSSHPHAAQQPTAIEL"
     gene            complement(2534..2782)
                     /locus_tag="PLG14_10770"
     CDS             complement(2534..2782)
                     /locus_tag="PLG14_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49658.1"
                     /translation="MVALIVGIVFIAFAVFACVPGPLGWWADVIAFLRGSLPVMALFV
                     GLIAVFIGVADIKDRVEAKKEEEEEAKEAAAKKDEKKA"
     gene            3137..>3557
                     /locus_tag="PLG14_10775"
     CDS             3137..>3557
                     /locus_tag="PLG14_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49659.1"
                     /translation="MIAAKSLALYKNRPVLVVDAGKDRLEIKLEDGSTAKVREKDLEA
                     LHPGPLSAVPAPASGGDFDTARAMAEGVLALPELAELVFGAAGPAEILACWKEGLEAA
                     RFRPAPGGLVAMSDEEVAKEAERRAKKEGEAAEKAAFV"
BASE COUNT          556 a         1170 c         1260 g          571 t
ORIGIN      
        1 ggctgaactg tagcacgcgc ctccccctac ccgcaaggct cagggcggcg ttccgagggg
       61 ttccggggcg gcgggccggg ccctcgtccc gccgcggtcg accgagggac agttgccgga
      121 gggaaagccg ggagcgccgc gcggccccgc cggcaaaaaa agagcggcgc cccggaaggc
      181 gccgcctgtc catgccccct cgccgggcct actggtcgtc ctgcttgatg aggagctcgt
      241 agacctccgc gggtgtcttc gccgcgagga gggaggtcct gagctccgtg gacttgagct
      301 tggagctgaa gaaggagagg gtctggaggt agtaggcgtg ctggttgtag gccgcggcga
      361 tcatgaagag gaggcggacg ggcacgtcgt cgaggctctg gaagtcgatg atgtcgcact
      421 tcgagaggcc gacggagacc acgaggtcgg tgaccgagga gagccgcacg tgggggatgg
      481 cgatgcccct gccgatcgcg gtcgacatga gctcctcgcg gcggatgatc tcggagacga
      541 gctcctggcg gttcttgatc tgcggggccg tcgcgaggtt ctcggaaagg gcgacgaggg
      601 cgtcgcgctt cttggagtgc tcgaggaaga ggatccggtc gggggagagg atgttctcta
      661 tctggacgac gctgaagacg gaggcgggct tgttcgagga aagccgctcg ttgacccagc
      721 gctcgatctc gctcttcttg aagcgccaga ccgtgccgat cttgccggaa gggatctctc
      781 ccttctgggc ccagtcgtag acggtgcgct cggagacgcg gagatagcgt gctacctctt
      841 caatggtgag gatatcatca tccatgcgaa ggactcctcc ggaacttcat cattccgcat
      901 tattgtgcaa cgaatgctag gcaatgtcaa gtgaagaagt ctgcaccccg ccccgccggg
      961 ccttgactac cctgccctac tcccctagag cctcgaggca ggccctgatc tccgcggcct
     1021 tgaaaccctg ggcgcgcagg gcctgggccg cccggtcccg gtcgccctcg gcccggccga
     1081 gctcgcggcg ggcagccttc gcgagggcgg agtcgcgctc ctcggggccc aggacctcgg
     1141 ccagggccgc cttggcggcc tcctcgccga tgcccctggc gcgcagggcg aggagaagcc
     1201 tggagggccc ctcgcccttc ttgaggcggg tcctgagcca ggcctcggcg tagcgccggt
     1261 cgtcgaggag gccttccgcg gccagccggt ccaaggccag ggcgacggcg cggcggccca
     1321 gctcgcgctt ggcgagcttc atctccaggc ccaggcggca ctgctcggcc ctggccagga
     1381 gggcgagggc gcggcgctcg gcctccgtcg cctccgcggc cagggagagg gcctccgccc
     1441 cggactcctc gagctcgagg ccgggaacga gggcctcggg atctaggccg agggcggcga
     1501 gatagcggaa ggcgacaaaa aaggaagagc ccccggccgc gaaggtcgcg gacccggagg
     1561 ctccctttcg tacggattcg atccgcacgg ctcgccgttt agcgcttgga gaactggaag
     1621 cgcctgcggg ctccggcctg gccgtacttc ttgcgctcga ccattcgcgg gtcgcgggta
     1681 aggaagccgt tggccttgag ggcggtccgg ttggccggat cgacctgggt gagggcgcgg
     1741 gccacgccgt ggcggcaggc gccggcctgg ccgttggggc cgccgccgag caccttgatg
     1801 aggatgtcgt acttgttttc ggaggccgtc accatgaggg gctggcggac gatggtcacg
     1861 agctcgccga ccttgaagta cttgtcgagt tcctggccgt tgacgacgac cttgccagtg
     1921 ccctcgcgaa ggaagacgcg agccacggcg cacttgcgcc gaccggttcc gataccgaga
     1981 ttcctgatca tccctatccc cttcttagag ttcgatggcg gtcggctgct gggccgcgtg
     2041 cgggtggctc gaaccggcgt agaacttgac gttcttgtag agtttccggc cgaggggacc
     2101 cttcgggagc atgccataga cggcgcgctc gagcggctcg gtcgggtggc gctcgatgag
     2161 ctgcttgaac gtgaagtcct tgagtccgcc cgggaagccc gtgtggtggt agtacatctt
     2221 gttctgccgc ttgcggccgg agatagcgac cttgtcggcg ttgatgacga ccacgaagtc
     2281 cccagtctcc tgggagggtt cgtaggtcgg cttgttctta ccgcggagca tcgaagcgac
     2341 cttggcggcg acgcggccca ggggcttgcc ctcggcgtcg atcacgtacc aggagcgcgt
     2401 ggcctcatcc ggcgtgacaa aaacggtctt catgtatgta taccttcacc ttcgcatggt
     2461 atcgttgcgg atttaatgcg cggagtatat tcccatatac ccggaatcgt gtcaagactt
     2521 ccgcccgggg gcgtcaagcc ttcttctcgt ccttcttggc ggcggcctcc ttggcctcct
     2581 cttcctcctc cttcttggct tccaccctgt ccttgatgtc ggcgacgccg atgaagactg
     2641 cgatgaggcc gacgaagagg gccatcacgg gcagggagcc cctgaggaag gcgatgacgt
     2701 cggcccacca gcccaagggg ccggggacgc aggcgaagac cgcgaaggcg atgaagacga
     2761 tgccgactat aagggcgacc attggctgtt cctccttgcg tcaagggcgc ccgactacaa
     2821 ggacgccgac ctagatcata gcatctcgcg cggcggaagg caacgggcgg ggctttccgc
     2881 ggaatagggc aaggcccgcg cccgtcgccg cgactgacat cggccgcggg gcggcgccga
     2941 agtccgcagg gcggcggcgc cgacgcccgc caggcggacc aagcccgccg ccgcgactga
     3001 aagcggccgc ggggcggcgg cgccgccccc ggccagccca aacctccccg ccgcgccccc
     3061 gtccggaaaa gcccgcccct tccgcgagcc tcgggccccg gggtatactc gcgccacccc
     3121 gctccgagga gcctccatga tagccgcgaa gtccctggcc ctctacaaga accgccccgt
     3181 cctcgtcgtc gacgcgggaa aagaccgcct cgagatcaag ctggaggacg gctcgacggc
     3241 caaggtgcgg gagaaggacc tcgaggccct ccatcccggc cccctctccg ccgttccggc
     3301 ccccgcctcc ggaggggact tcgacacggc ccgcgccatg gccgaggggg tcctcgccct
     3361 ccccgagctc gccgagctcg tcttcggcgc ggctggcccc gcggagatcc tcgcctgctg
     3421 gaaggagggc ctcgaggccg cccgcttccg gcccgccccc ggaggcctgg tcgccatgag
     3481 cgacgaggag gtcgcgaagg aggccgagag gcgggcgaaa aaggagggag aggcggcgga
     3541 aaaggcggcc ttcgtcc
//
LOCUS       DAOOSJ010000253         3548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16954_length_3548_cov_4.125966, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000253 DAOOSJ010000000
VERSION     DAOOSJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16954_length_3548_cov_4.125966"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1592)
                     /gene="murA"
                     /locus_tag="PLG14_10780"
     CDS             complement(309..1592)
                     /gene="murA"
                     /locus_tag="PLG14_10780"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOX49660.1"
                     /translation="MYKYIIEGGFPVKGRVRASGNKNAALPCIAATLLSAEPVVLRNI
                     PEIEDVQVMLEVLRKLGASVERLGPNDWRVDCGPVSRSEVPAAEAKKVRASILFAGPL
                     LARFGKVTLPPPGGDTIGRRRIDTHVLALEELGARFDIDGALKFTANKLVGADLFLDE
                     ASVTGTENAVMAAVLAEGRTVIRNAASEPHVQDLCEFLKAMGAKIEGSGSNLLRIEGV
                     KALHGADFSIGPDFMEIGSFIGLTAVTRGELVIEGVRPEELRPLKVAYGRLGIKWSVE
                     GDELLVSSGQSMKVQEDLGGQVPKIDDSPWPGFPPDLTSIMTVVATQTEGEVLIHEKM
                     FESRMFWVDKLIGMGARIVLCDPHRAVVSGPSRLKADSLTSPDVRAGMAMVIAALCAE
                     GKSTIHNVYQIERGYENISERLRALGAHIERLEDC"
     gene            1861..1995
                     /locus_tag="PLG14_10785"
     CDS             1861..1995
                     /locus_tag="PLG14_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49661.1"
                     /translation="MPYKLDGAKFSTIEELIEALYPLYADKMSEEEFRKYAEEKAEKT
                     "
     gene            1995..>3548
                     /locus_tag="PLG14_10790"
     CDS             1995..>3548
                     /locus_tag="PLG14_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOX49662.1"
                     /translation="MPNEDFPGADERRGIRHSVRTFLNHIAGYGDILRQEAEDSGDAG
                     LCETYDGIRQAALELRELALPFFLEPSEGGIAEEDRPERERAIYGALYDIIALIQGAK
                     RRGDAEWTSRFLPDTEKILEAANGIVEIFEERLEAHRDFVEEYEPEALPVGLGDVELP
                     PAPKAGRILVVDDNLFNRELLGRHLERQGHVVCQAADGASALEILRHALFDVLVLDVM
                     MPGMNGYQLLEAVKADERLKDLYVIVISALDDTKSIARCIQLGAEDYLPREFEPVILK
                     ARIESCLEKKSLKDKEELYVAAVMETERRLRAELQEGAAYVRGLLPPRLPGPGLKSDW
                     VFIPSLSLGGDVFGYHAIEGGRVALYLIDVSGHGIEAALFSVTLMNMLKTQVLPRADF
                     GEPSSVLERLNASFRMEEQNNLYFTAWYGVWDPATRELAYASAGAPPAVLLLPGGGVE
                     QLETGGMIVGVDEGAEYRSERMVLPAGSSLYLFSDGIYEIRTQDGSVLGIEAFVGLLA
                     GLAARGRGPL"
BASE COUNT          540 a         1228 c         1258 g          522 t
ORIGIN      
        1 cagccctcga gccctacccg cgccgtccgg gccgcaggcc cggacgcgtg ccgcgcacca
       61 agcgtcagcc ctcgagcccc accccgcgcc gtccaggccg caggcctgga cgcgtgccgc
      121 gcaccaagcg tcagccctcg agccccaccc cgcgccgtcc aggccgcagg cctggacgcg
      181 tgccgcgttg caggcgacac ccacgcggca ggcaggtcca gggcagcgcc ctggtcgcgc
      241 ctttgaggaa agaggggcct ggggagaaaa cccctttcgc tcgaaacgaa aggggttttc
      301 tccccagatc agcaatcctc cagccgctcg atgtgcgcgc ccagggcgcg gagccgctcg
      361 gagatgttct cgtagccgcg ctcgatctgg tagacgttgt ggatcgtgct cttgccctcg
      421 gcgcagaggg cggcgatcac catggccatg ccggcgcgga cgtcggggct cgtgagggag
      481 tcggccttga gccgggaggg gccggagacg acggcgcggt gcgggtcgca gaggacgatg
      541 cgcgccccca tgccgatgag cttgtccacc cagaacatcc gggactcgaa catcttctcg
      601 tggatcagga cctcgccctc cgtctgggtc gcgacgaccg tcatgatgga ggtgaggtcg
      661 ggcgggaagc cgggccaggg cgagtcgtcg atcttcggca cctggccgcc caggtcctcc
      721 tggaccttca tggactggcc tgaggagacg aggagctcgt ccccctcgac gctccacttg
      781 atgcccagac ggccgtaggc gaccttgagg ggcctgagct cctcgggcct gacgccctcg
      841 atgacgagct cgccgcgggt gacggcggtg aggccgatga aggagccgat ctccatgaag
      901 tcggggccga tggagaagtc ggcgccgtgg agggccttca cgccctcgat cctgaggagg
      961 ttggagcccg agccctcgat cttggcgccc atggccttga ggaattcgca gaggtcctgg
     1021 acgtggggct cgctcgcggc gttcctgatg acggtgcggc cctctgcgag gacggcggcc
     1081 atcacggcgt tctccgtgcc ggtgacggag gcctcgtcca ggaagaggtc ggcgccgacg
     1141 agcttgttcg ccgtgaactt gagggccccg tcgatgtcga agcgggcgcc cagctcctcg
     1201 agggccagga cgtgggtgtc gatgcggcgg cggccgatcg tgtcgccgcc ggggggcggg
     1261 agggtgacct tgccgaagcg cgcgaggagg ggtccggcga agaggatgga ggccctgacc
     1321 ttcttcgcct cggcggcggg gacttccgag cggctcacgg ggccgcagtc gacgcgccag
     1381 tcgttcggcc cgaggcgctc gacggaggcg ccgagcttgc gcaggacctc gagcatgacc
     1441 tggacgtcct cgatctccgg gatgttgcgg aggacgacgg gctcggccga gaggagggtc
     1501 gcggcgatgc agggcagggc cgcgttcttg ttgccgctcg ccctgacgcg gcccttgacc
     1561 gggaagccgc cctcgatgat gtacttgtac atgggcgcta ctctacggga gaaggcgcaa
     1621 agggtcaacc gacggcgagg gcgggcggcg ggcccgaggg cgcagcgtgt gccctgggcc
     1681 ggccggcgcc cggccgaggc gcgcgggcgg gcggacccgc ggaccggggc gccgccgcgg
     1741 ggccggccgc gcgcggacct ccgcccggcg cgggaatttc ttgcagtcgg gcgtggttcc
     1801 gtactactat tggaggctgg cggcctcgac ccgatccggc tcgccatccg agaggtttcc
     1861 atgccctaca agctcgacgg cgccaaattc tccacgatcg aggagctcat cgaagccctc
     1921 tatccgctct acgctgacaa gatgagcgag gaggaattcc ggaagtacgc ggaagagaag
     1981 gccgagaaaa cctgatgccg aacgaggact tccccggcgc cgacgagagg aggggcatca
     2041 ggcactccgt ccgcaccttc ctgaaccaca tcgcgggcta tggggacatc ctcaggcagg
     2101 aggcggagga ctcgggcgat gccgggctct gcgagaccta cgacggcatc cggcaggcgg
     2161 ccctggagct ccgcgagctc gccctgccct tcttcctcga gccctccgag ggcgggatag
     2221 ccgaggagga caggcccgag cgggagcggg ccatctacgg cgccctctac gacatcatcg
     2281 ccctgatcca gggcgccaag cgccggggcg acgccgagtg gacctcgcgc ttcctccccg
     2341 acacagagaa gatcctggag gccgccaacg gcatcgtcga gatcttcgag gagaggctgg
     2401 aggcccatcg ggacttcgtc gaggagtacg agcccgaggc cctgccggtc gggctgggcg
     2461 acgtcgagct cccgcccgcg cccaaggcgg ggcggatcct ggtcgtggac gacaacctct
     2521 tcaaccgcga gcttttgggg cggcacctgg agcggcaggg ccacgtcgtc tgccaggcgg
     2581 ccgacggcgc ctcggccctg gagatcctca ggcacgcgct cttcgacgtc ctggtcctgg
     2641 acgtgatgat gcccggcatg aacggctacc agctcctgga ggccgtcaag gccgacgagc
     2701 ggcttaagga cctctacgtc atcgtgatct cggccctgga cgacacgaag agtatagccc
     2761 gctgcatcca gctgggcgcg gaggactacc tgccccgcga gttcgagccg gtgatcctca
     2821 aggcccgcat cgagtcctgc ctggagaaga agagcctcaa ggacaaggag gagctctacg
     2881 tcgccgcggt gatggagacc gagcgccgcc tccgcgcgga gctccaggag ggcgcggcct
     2941 acgtgcgcgg cctcctgccc ccgcgcctgc ccggcccggg gctcaagtcg gactgggtct
     3001 tcatcccctc gctctcactg ggcggcgacg tcttcggcta ccacgccatc gagggcggcc
     3061 gcgtggccct ctacctcatc gacgtctccg gccacggcat cgaggcggcc ctcttctccg
     3121 tcaccctcat gaacatgctc aagacccagg tcctgccccg cgcggacttc ggcgagccct
     3181 cctccgtcct cgagcggctc aacgcgtcct tccgcatgga ggagcagaac aacctctact
     3241 tcaccgcctg gtacggcgtc tgggatcccg cgacgaggga gctcgcctac gcctccgccg
     3301 gagcgcctcc cgccgtcctc ctcctgcccg gcgggggcgt cgagcagctg gagaccggcg
     3361 gcatgatcgt cggcgtggac gagggggccg agtaccgctc cgagcggatg gtcctccccg
     3421 ctggctcgag cctctacctc ttctccgacg gcatctacga gatcaggacg caggacggct
     3481 ccgtcctcgg catcgaggcc ttcgtcggcc tactcgccgg cctggccgcg cggggccgcg
     3541 gccccctc
//
LOCUS       DAOOSJ010000254         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_16988_length_3544_cov_5.230152, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000254 DAOOSJ010000000
VERSION     DAOOSJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16988_length_3544_cov_5.230152"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLG14_10795"
     CDS             <1..474
                     /locus_tag="PLG14_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOX49663.1"
                     /translation="KLIEYQKYKRLSELMEKKEMEVEWAIERKKMQRPLPFAEEASLW
                     EEIDVWDLLKTFSSLVTNLTSERIIDLYEEVSINEKIALIHELLDSKESFSFTELITR
                     PRSTMDVACAFLAILEAVKFRIITIYQHRLFGDIAIRRRAETEAAATAEDHGTEA"
     gene            458..988
                     /gene="scpB"
                     /locus_tag="PLG14_10800"
                     /pseudo
     CDS             458..988
                     /gene="scpB"
                     /locus_tag="PLG14_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            1251..1985
                     /locus_tag="PLG14_10805"
     CDS             1251..1985
                     /locus_tag="PLG14_10805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX49664.1"
                     /translation="MAETESGPVRLQAWLARAGVASRRAAERLILEGRVSVNGSVVTE
                     LGTKVGPGDAVLFDGKRLSLEERKRYILLNKPAGLLSAMSDPEGRPLAVDLLKPEVEE
                     RVYNVGRLDQWSAGLLLFTNDGELASLLGHPSGGIEKEYEVETDLDLPDDFLPAFLRG
                     IVVEGIRYAALRAARTGPRSARIVLVEGKNREIRRVLAARNLRALSLRRVRIGPLAIG
                     DLAEGAHRELSPAEVAALYAAASRKD"
     assembly_gap    1996..2095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2125..>3544
                     /gene="cmk"
                     /locus_tag="PLG14_10810"
     CDS             2125..>3544
                     /gene="cmk"
                     /locus_tag="PLG14_10810"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOX49665.1"
                     /translation="MIVAIDGPAGSGKSTIARMLAEGLHFVYVNSGNLYRALTLGAIR
                     RGLDEKAPEGVLAYARGAGIEYRGGRLFLDGEDVEDLLHSAPVDALVAQLSAIPALRD
                     IVNAQVRKVAAGMDAVVEGRDMTTVVFPEAKYKFFLDASPEARARRRLGQGTSGDSLE
                     EIARNIAMRDDIDRAKSVGALKIAPDAYYLDSSDLTLQEVYVNLCGKILHQGNHMGVK
                     EVEMGAPAPSRDDIQTQLQEQYLKSLEGLEEGDLVDGHVIQVTSDFVFVDVGYKSEGK
                     IPVIEFGENVPKVGDVVSVILVKKEARNGEIVVSKKRADEKVYWRQVSNAFKDHLPLE
                     GTIDKEIKGGFEVNLGHGLRGFLPASKADVQRIEKGDKLVGLKTSFYLERLYSEKKVN
                     IVLNRRKWMEEDIEKHRSEFFANTQIGDSVKGVVKSFTSFGAFIDLGGFDGLLHINDM
                     SWGHVTRPKDFVKKGQEIELKVI"
BASE COUNT          633 a         1210 c         1100 g          501 t
ORIGIN      
        1 aagctcatcg agtaccagaa gtacaagcgc ctctccgagc tcatggagaa gaaggagatg
       61 gaggtcgagt gggccatcga gcggaagaag atgcagcgcc cgctcccctt cgcggaggag
      121 gcctcgctct gggaggagat cgacgtctgg gacctcctca agaccttctc ctccctcgtc
      181 acgaacctca cctccgagcg gatcatcgac ctctacgagg aagtctccat caacgagaag
      241 atcgccctga tccacgagct cctggactcg aaggagtcct tctccttcac cgagctcatc
      301 acgaggccgc ggtccacgat ggacgtggcc tgcgccttcc tggcgatcct cgaggcggtg
      361 aaattccgca tcatcacgat ctaccagcat cgcctcttcg gcgacatcgc catccgccgc
      421 agggcggaga cagaggccgc ggcgacggcg gaggaccatg gaactgaggc ttgagcgcga
      481 ggcggccatc ctggagaccg tcctcttcct ggaatcggag ccgatggacg aggccgccct
      541 ctcgcgggcg acgggcctgg accgcgaggt cgtcgagcgt gccctggagg tcctggccga
      601 ggagtacgcg ggcgcggccc acggcctcga gcccctgcgc tcggggggcg gctggatcct
      661 cgcgcccaag gccgagcact gggagtccct gaaggagcgc tacggcaaga aggccgactc
      721 ccgcctctcc cgggccgccc tggagaccct ctccatcgtc gcctactccc agcccatcac
      781 ccgcgccgag gtggaggcca tccgcggcgt ctccgcggac ggcatgatcc gcttcctcct
      841 cgagaaggcc ttcatcaagg aggtcggcaa gaaggaagcc cccggccggc ccgtccagta
      901 cgggacgacg aaggaattcc tcaagtactt ccgcctcggg agcatctccg acctccccaa
      961 gctcgacgag ctcgagcgcg agcgcttcgg cggcgacgag gcctccgagg ctcccgccat
     1021 aggccccttc atcgccggcg cctccctcgt ggaggccggc gcagaggccg ccgccgccag
     1081 ggccggcgcg gccatccacg ccgccgccga gcgcgccgcg gaggccgagg ccgaggccga
     1141 ggcggccgag tccgaggtcg ccacagccgc gtcagaggcc ggcgcggccg ccgccgatga
     1201 agaccacgac gcagacggcg cgagcgccgc ggccgaggaa caagcgcacg atggccgaaa
     1261 ctgagtccgg tcccgtccgc ctccaggcct ggctcgcccg ggccggcgtc gcctcgcggc
     1321 gcgcggccga gcggctcatc ctggaaggcc gcgtctcggt caacggctcc gtcgtcacgg
     1381 agctcggcac caaggttggc cccggcgacg ccgtcctctt cgacggcaag cgcctctccc
     1441 tggaggagag gaagcgctac atcctcctca acaagccggc cggcctcctc tccgccatgt
     1501 ccgaccccga gggcaggccc ctcgccgtcg acctcctcaa gcccgaggtg gaggagcggg
     1561 tctacaacgt cggccgcctg gaccagtggt ccgccggcct cctcctcttc acgaacgacg
     1621 gcgagctcgc ctccctcctc ggccatccct cgggagggat cgagaaggag tacgaggtgg
     1681 agacagacct cgacctcccg gacgacttcc tccccgcctt cctgcgcggc atcgtggtcg
     1741 aggggatccg ctacgcggcc ctgcgcgcgg ccaggaccgg gccccgctcg gcccggatcg
     1801 tcctcgtgga gggcaagaac cgcgagatcc gccgcgtcct cgccgcgagg aacctgaggg
     1861 ccctctccct gaggcgcgtc aggatcggtc ccctcgcgat aggcgatctc gccgaggggg
     1921 cccaccgcga gctcagcccc gcggaggtcg ccgccctcta cgccgccgct tcccgcaagg
     1981 attaggcgcg gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttagg
     2101 caaatctttc accaggaagg acttatgata gtcgcgatcg acggcccggc gggatccggg
     2161 aaaagcacca tcgcgcggat gctcgccgag ggtctccatt tcgtgtacgt caactcgggc
     2221 aacctctacc gggccctcac cctgggcgcg atccgccgcg gcctggacga gaaggccccc
     2281 gagggggtcc tggcctacgc ccgcggggcg ggcatcgagt acaggggcgg ccggctcttc
     2341 ctggacggcg aggacgtcga ggacctcctc cattcggccc ccgtggacgc cctcgtggcc
     2401 cagctctccg ccattcccgc cctccgcgac atcgtgaacg cccaggtccg caaggtcgcg
     2461 gccggcatgg acgccgtcgt cgagggcagg gacatgacca ccgtcgtctt ccccgaggcg
     2521 aagtacaagt tcttcctgga cgcgagcccc gaggccaggg cgagacggcg cctgggccag
     2581 gggacgagcg gcgactccct cgaggaaatc gcccggaaca tcgcgatgcg ggacgacata
     2641 gaccgggcca agtccgttgg cgccctcaaa atcgcgcccg atgcctacta tttagacagc
     2701 tcggacttga ccttacagga agtttatgtc aatctgtgcg ggaaaattct tcatcaaggg
     2761 aaccatatgg gcgtgaagga agtggaaatg ggtgctcccg ctccctcccg ggacgacatc
     2821 cagacacaat tgcaggaaca gtatctcaag agcctggaag gacttgaaga gggtgacctc
     2881 gtagatggtc acgtcatcca ggtaaccagc gatttcgtct tcgtggacgt aggctacaag
     2941 tccgagggca agatccccgt catcgagttc ggggagaacg tccccaaggt cggcgacgtc
     3001 gtcagcgtca tcctcgtcaa gaaagaggcc cgcaacgggg agatcgtggt ctccaagaag
     3061 cgcgccgacg agaaggtcta ctggcgccag gtctccaacg cgttcaagga tcaccttccc
     3121 ctggaaggca cgatcgacaa ggagatcaag ggcggcttcg aggtcaacct cggccacggc
     3181 ctccgcggct tcctcccggc ctccaaggcc gacgtacagc gcatcgagaa gggcgacaag
     3241 ctcgtcggcc tcaagacctc cttctacctc gagcgcctct actccgagaa aaaggtcaac
     3301 atcgtcctca accgccgcaa gtggatggaa gaggacatcg agaagcaccg cagcgagttc
     3361 ttcgcgaaca cgcagatcgg cgacagcgtc aagggcgtcg tgaagagctt cacgagcttc
     3421 ggcgccttca tcgacctcgg cggcttcgac ggcctcctcc atatcaacga catgagctgg
     3481 ggacacgtga cccggcccaa ggacttcgtc aagaagggcc aggagatcga gctcaaggtc
     3541 atcc
//
LOCUS       DAOOSJ010000255         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17012_length_3541_cov_5.955250, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000255 DAOOSJ010000000
VERSION     DAOOSJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_3541_cov_5.955250"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="metK"
                     /locus_tag="PLG14_10815"
     CDS             complement(<1..653)
                     /gene="metK"
                     /locus_tag="PLG14_10815"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOX49666.1"
                     /translation="MKDRRYLFTSESVGEGHPDKLCDQVSDAVLDACLAKDPQSHVAC
                     ECYATTGMILVGGEITTDTYVDIQALARDVVKEIGYTNPDYGIDYESMAVLNTIHAQS
                     QDIAQGVDGHGLKEFEGQQGAGDQGMMFGFACRETPELMPAPIMYAHRILERAAELRK
                     ARRISWLRPDAKSQVTLEYEGHKPVRVDTVVVSHQHDPDIPYEALRDTVIEEIIRPIL
                     "
     gene            893..1882
                     /locus_tag="PLG14_10820"
     CDS             893..1882
                     /locus_tag="PLG14_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOX49667.1"
                     /translation="MSTTCWKKGRASRAAPAALALGLAAALGAYAEAPILAAKPKEAR
                     ARVMDAALAELGAPYLYGGADDKGFDCSGLVYRAYADALGAALPRTARSLYSYAERIE
                     RGELQPGDLVFFDTTGRLAHVGLYLGEGRFVHAASEGPRVGVIESSLGEAYWARSYAG
                     AGRILSPAEYLGLVLRAALGPSLGASPLARGASGAAGLSFRALGLEFGLEARPEYDAA
                     LAAGRLPLVLTVGLDRSLRVFAGPALTLGSPRLEGPEGAAARAYHAEGGFLGTAGLTW
                     TPLRFSAAGLDWGLYAELVYNRYVADEGQADDAMADLRARVRAGAGLSARFSF"
     gene            complement(1910..2509)
                     /locus_tag="PLG14_10825"
     CDS             complement(1910..2509)
                     /locus_tag="PLG14_10825"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HOX49668.1"
                     /translation="MKTKHVLYGFLALMAGLILVAVFFPAAIETLLNQPALSGHALFV
                     HVVSTTLFFGNAVVGMLWEWRSLKSGRKEVFLHTYGTVSWLDARLSAPLIVLSVLSGV
                     TLSLILGDLWRIGWLSLGFALFLLSGLVWVLSDIPTQYRVKRLVAGLEPGDEALPPEL
                     VRLLRLRLRISLAGVLPLAVVFALMVYRPELPPLAQWFR"
     gene            2731..>3541
                     /locus_tag="PLG14_10830"
     CDS             2731..>3541
                     /locus_tag="PLG14_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOX49669.1"
                     /translation="MAAKERYILALDQGTTSSRAILFNKSGLIIGVKQIQFTQIFPKP
                     GWVEHDPEEIWSTQLEAARGAIEAAQIEADQIAAVGITNQRETTVLWDRATGRPVHNA
                     IVWQCRRSADICQELRALGLSDLVRGKTGLVLDPYFSGTKLMWLFAEYPELRRRAEKG
                     ELMFGTVDSWLIYNLTGKHMTDPTNASRTLLFNIREGKWDEELLRLFKIPASILPEVA
                     PSSSSFGTVRKELFGAEIPVAGCAGDQQAALFGHACYEPGMAKNTYGTGCFT"
BASE COUNT          545 a         1158 c         1318 g          520 t
ORIGIN      
        1 aggatggggc ggatgatctc ctcgatgacg gtgtcgcgga gggcctcgta ggggatgtcg
       61 gggtcgtgct ggtgggagac gacgacggtg tcgacgcgga cgggcttgtg gccttcgtac
      121 tccagggtga cctggctctt cgcgtcgggg cggagccagg agatgcggcg cgccttgcgg
      181 agctcggcgg cgcgctccag gatgcggtgt gcgtacatga tgggcgccgg catgagctcc
      241 ggcgtctccc tgcaggcgaa gccgaacatc atgccctggt cgcccgcgcc ctgctggccc
      301 tcgaactcct tgaggccgtg gccgtcgacg ccctgggcga tgtcctggga ctgggcgtgg
      361 atggtgttga ggacggccat ggactcgtag tcgatgccgt agtcggggtt ggtgtagccg
      421 atctccttga cgacgtcgcg ggcgagggcc tggatgtcga cgtaggtgtc ggtggtgatc
      481 tcgccgccga cgaggatcat ccccgtcgtc gcgtagcact cgcaggcgac gtggctttgc
      541 ggatccttgg cgaggcaggc gtcgaggacg gcgtccgaga cctggtcgca gagcttgtcc
      601 gggtggcctt cgccgacgga ctcggaggtg aagaggtagc gtctgtcttt catttcgcgc
      661 tccttgcgct catggaggcg ccggaacgga cggacggttg gacacgggcg accacgtcgg
      721 gcctcctttt tagcctgcat ttcttgaccg cccgcaatag ggaacaactc ggcggtacgg
      781 ttcccgcgcg acgcgccgga gctccgtgcc tccactatcg cggatcgggc cgggagaggc
      841 aagggggcgg gggcccagga tggcggcggc cgaggcgagg tatagttatg gcatgtcgac
      901 gacgtgttgg aagaagggaa gggcgtcgcg ggccgcgccg gcggcgctgg ccctgggcct
      961 ggccgcggcc ttgggggcct acgcggaggc gccgatcctg gcggcgaagc ccaaggaggc
     1021 gagggcgagg gtcatggacg cggccctggc cgagctcggc gctccctacc tctacggcgg
     1081 cgccgacgac aagggcttcg actgctcggg cctggtctac cgggcctacg ccgacgccct
     1141 gggcgcggcc ctgcccagga cggcgcgctc gctgtactcc tatgccgagc ggatcgagcg
     1201 cggcgagctc cagccgggcg acctcgtgtt cttcgacacg acggggcggc tcgcccacgt
     1261 cgggctctac ctgggcgagg gacgcttcgt ccacgccgcc tcggaggggc cgcgcgtcgg
     1321 cgtcatcgag tcgagcctgg gagaggccta ttgggcccgc agctacgcgg gcgcgggccg
     1381 gatcctctcg ccggcggagt acctgggcct ggtcctgcgc gcggccctgg ggccgagcct
     1441 gggcgcctcg cccctcgcgc ggggcgcctc aggcgcggcg ggcctgagct tccgcgccct
     1501 ggggctggag ttcggcctgg aggcgcggcc ggagtacgac gcggccctgg cggcggggcg
     1561 cctgcccctg gtcctgacgg tggggctgga ccgctccttg cgcgtcttcg ccgggcctgc
     1621 gctgaccctg ggaagcccgc ggctggaagg cccggaggga gccgccgcgc gcgcctacca
     1681 cgccgagggc ggcttcctgg gcacggccgg cctcacctgg acgcccctgc gcttcagcgc
     1741 cgcgggcctg gactgggggc tctacgcgga actcgtctac aaccgctacg tcgcggacga
     1801 gggccaggcg gacgacgcca tggccgacct ccgggcgcgg gtcagggcgg gggcgggcct
     1861 aagcgcgcgg ttcagcttct agggtcgggg cggaccgccc aagagcggct caacggaacc
     1921 actgggccag gggcggcagc tccggcctgt acaccatcaa ggcgaagacg accgccaggg
     1981 gaaggacccc cgccaaggag atccgcaagc gcagccgcaa gaggcggacg agttccgggg
     2041 gaagggcctc gtccccgggc tcgaggccgg ccaccagccg cttcacccgg tactgggtcg
     2101 ggatgtcgct caggacccag accaggccgg aaagcaggaa gagcgcgaag cccagggaca
     2161 gccagcctat ccgccagagg tcgcccagga tcagtgagag ggtcaccccc gacaggacgg
     2221 agaggacgat gagcggcgcc gagaggcggg cgtcgagcca ggacacggtg ccgtaggtat
     2281 ggaggaagac ctccttccgg ccggacttga ggcttctcca ttcccacagc atgccgacga
     2341 ccgcgttgcc gaagaacagc gtcgtcgaga cgacgtggac gaagagggca tggccggaca
     2401 gggccggctg gttgagcagg gtttcgatcg ccgccgggaa gaagacggcg acgaggatca
     2461 atcccgccat gagggccagg aaaccgtaga ggacgtgctt ggtcttcatg cttgccaatt
     2521 ggagcatggc gcgcgccgga aggcaacagc ggctctgcct tgcccggctc gtcctcgggt
     2581 tctcgcgccc ttgggggccg gaatttccgc gggcgtgcgc cgggcggccc gagcaacgcg
     2641 gggcggtgtt caaaatgcag ggataaaatg cctctgcaca gggcggggaa agggacgtat
     2701 actggcggca ctctatttcc ggaggaccgc atggccgcca aggaacgcta catactcgcc
     2761 ctggaccagg gcacgacctc gtcgagggcc atccttttca acaagtccgg cctcatcatc
     2821 ggcgtgaagc agatccagtt cacgcagatc ttccccaagc cgggctgggt cgagcacgac
     2881 cccgaggaga tctggtccac ccagctcgag gcggcgcgcg gcgccatcga ggccgcccag
     2941 atcgaggccg accagatcgc cgcggtcggg atcacgaacc agcgcgagac gacggtgctc
     3001 tgggacaggg cgacggggag gcccgtccac aacgccatcg tctggcagtg ccgccgctcc
     3061 gcggacatct gccaggagct ccgcgccctg ggcctctccg acctggtgcg cgggaagacg
     3121 ggcctggtcc tggatcccta cttctccggc acgaagctca tgtggctctt cgccgagtac
     3181 cccgagctca ggcggcgcgc ggagaagggc gagctcatgt tcgggacggt ggactcctgg
     3241 ctcatctaca acctgaccgg gaagcacatg accgacccga cgaacgcgag ccgcaccctc
     3301 ctcttcaaca tccgggaggg gaagtgggac gaggagctct tgcggctctt caagatcccc
     3361 gcctccatcc tccccgaggt ggcgccgagc tcctcgagct tcggtacggt caggaaggag
     3421 ctcttcggcg ccgagatccc ggtggcgggc tgcgccggcg accagcaggc cgccctcttc
     3481 ggccacgcct gctacgagcc gggcatggcg aaaaacacct acggcacggg ctgcttcacc
     3541 c
//
LOCUS       DAOOSJ010000256         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17160_length_3518_cov_5.900375, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000256 DAOOSJ010000000
VERSION     DAOOSJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17160_length_3518_cov_5.900375"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLG14_10835"
     CDS             <1..629
                     /locus_tag="PLG14_10835"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOX49670.1"
                     /translation="VAWGACIKASQLGAKVVTISGPDGYIFDPDGIGTQEKWDYLNEM
                     LVIDRNKVETYAKKFPNAKFFAGRKPWEQKVDIAMPCAIQNELNGEDAKTLLANGVKI
                     VAEVSNMGCTPEAWKLFVEKKIPFGPGKAVNAGGVATSGLEMSQNSMRLAWSAEEVDE
                     RLHAIMVNIHKAAYDTAKQYGFEGNYVVGANIAGFKKVADAMIAQGLV"
     gene            complement(841..1752)
                     /locus_tag="PLG14_10840"
     CDS             complement(841..1752)
                     /locus_tag="PLG14_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49671.1"
                     /translation="MLKKKRRDGSLVPELPAFTKIMPYLMPDKTGSIIFYQEDYDVTE
                     ALAFVKAWNRRRMREEGTILTLFEVVLCAAARAVSMRPRVNRFIANRRYYQRNQLILS
                     FVAKKELTDEGKEVNVKAVFGAGETLATTARKVKALVKKGISEDGHDNEKIVDAVMRL
                     PAFLLKFMNFAYNWLDDHNLLPYAVTESDPMWSTIFLANVGSFGLDPPFHHLFERGTC
                     PVFMAVGKVRGENRLSAAPDGSPRVEERKILRINYSFDDRIADGIYMGRTLELVRGFI
                     ENPALLDEPAELPPEILAELALRPDKD"
     gene            1911..>3518
                     /locus_tag="PLG14_10845"
     CDS             1911..>3518
                     /locus_tag="PLG14_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOX49672.1"
                     /translation="MPRKHVFGLTAWGRYFVDAMEALGDPGRLERGRAYAGKGRVSSL
                     SIGNGLAKARVEGNYRPYYSVKISFPPFSAAERRGLEKLVGADPLELARVRAGELPPG
                     LLDHVERAGISLFPRRWSEMKRSCDCPDSGDPCKHEAAVYYVIAQEIDRDPSVLFRLR
                     GFDPAASSAPGELAGRAGPAAKPAADGEEPTVLEPLELREADTWTAPDPADYPAELPS
                     LASYGALLPRILPPGPALAGLDLEVALAEFYHCLSRDWEKPLAARPPASSKPRAALDE
                     EAGCEAAARSFALAGIRVAARGQRGEFILPGGARLSPLEAARLVLAMDLESGSPSYVF
                     LRRFSLAMRSIVAAGAFHPDIRAGDGRFSALWLPARFAADAREALAWVEAAAVEPERS
                     AGPRAAPAAGTRTSGKATLDPPSLVALFASTFLGDYVAALAFRPSGSLAAASPAAKAL
                     FGGEEVDASSPLLRSLPRALAARFSVYRLAARRSGFELAAKPAASRSGAPAYRLKASV
                     LAPEGRRLSLREAGAILGADALAFPALL"
BASE COUNT          496 a         1261 c         1191 g          570 t
ORIGIN      
        1 acgtcgcctg gggcgcctgc atcaaggcga gccagctcgg cgccaaggtc gtcacgatct
       61 ccggcccgga cggctacatc ttcgatccgg acggcatcgg cacccaggag aagtgggact
      121 acctcaacga gatgctcgtc atcgaccgca acaaggtaga gacctacgcc aagaagttcc
      181 cgaacgcgaa gttcttcgcg ggccgcaagc cctgggagca gaaggtcgac atcgccatgc
      241 cctgcgccat ccagaacgag ctcaacggcg aggacgctaa gaccctccta gccaacggcg
      301 tcaagatcgt cgccgaggtc tcgaacatgg gctgcacccc cgaggcctgg aagctcttcg
      361 tcgagaagaa gatccccttc ggccccggca aggccgtgaa cgcgggcggc gtcgccacct
      421 ccggcctcga gatgagtcag aactccatgc gcctggcctg gagcgccgag gaagtcgacg
      481 agaggctcca cgccatcatg gtcaacatcc acaaggccgc ctacgacacc gcgaagcagt
      541 acggcttcga gggcaactac gtcgtgggcg cgaacatcgc cggcttcaag aaggtcgccg
      601 acgccatgat cgcccagggc ctggtgtaac caggccgggt ctcgcgtaat cggatcccgt
      661 ttggaggggc ggccctgaaa agccgccacc cccttggaac tcgaagaggc cgcccgagag
      721 ggcggcctct ttttgcgttt gggggattct cgcgggggaa ggtggagcga gggcggcgcg
      781 ggccgctccg cgcccggcgc gtcccgcaag gagccgcgcc tcgcttggcc gcggctacgc
      841 ctagtccttg tccgggcgga gcgcgagctc ggcgaggatc tcgggcggga gctcggccgg
      901 ctcgtcgagg agggcgggat tctcgatgaa gcccctgacg agctccaggg tcctccccat
      961 gtagatcccg tcggcgatgc ggtcgtcgaa ggaatagttg atccgcaaga tcttccgctc
     1021 ctcgacccgg gggctcccgt ccggcgcggc ggagaggcgg ttctcgcccc gcaccttgcc
     1081 gacggccatg aagaccgggc aggtgccgcg ctcgaagagg tggtggaagg gcggatcgag
     1141 gccgaaggag ccgacgttcg cgaggaagat cgtgctccac atggggtcgc tctccgtgac
     1201 cgcgtagggc aggaggttgt ggtcgtcgag ccagttgtag gcgaagttca tgaacttgag
     1261 gaggaaggcg ggcagccgca tcaccgcgtc gacgatcttc tcgttgtcgt ggccgtcctc
     1321 ggagatgccc ttcttgacga gggccttcac cttgcgggcc gtcgtcgcga gcgtctcgcc
     1381 ggccccgaag accgccttga cgttgacctc cttgccttcg tcggtgagct ccttcttcgc
     1441 gacgaaggag aggatgagct ggttccgctg gtagtagcgg cggttagcga tgaagcggtt
     1501 cacgcggggg cgcatgctca cggccctggc ggccgcgcag aggaccacct cgaagagggt
     1561 gaggatggtc ccctcctccc gcatgcgccg ccggttccag gccttgacga aggccagggc
     1621 ctccgtaacg tcgtagtctt cctggtagaa gatgatcgat cccgtcttgt ccggcatgag
     1681 gtagggcatg atcttcgtga aggcgggcag ctcggggacg aggctgccgt ccctcctctt
     1741 cttcttgagc acgtcgacac tccttgggcg gagcccgccg cgggccccgg ccaaggagta
     1801 tggcgagggg ctcggggagc cgtcaaccgc gcggcctgcg gggaaacaga ggcggggacg
     1861 ggacactggg cggtcttgaa gcgggccgta gccttcgcta tccttcttgc atgccgcgca
     1921 agcatgtctt cggcctcacg gcctgggggc ggtacttcgt agacgccatg gaggcgcttg
     1981 gcgatccggg acggctcgag cgaggcaggg cctacgcggg caagggccgc gtgtcgagcc
     2041 tctcgattgg gaatggcctc gcgaaggccc gggtggaggg caactaccgg ccctactatt
     2101 ccgtgaagat ctcctttcct cccttctcgg ccgccgaacg gcgcggcctg gagaagcttg
     2161 tcggggcgga tcccttggaa ctcgcccgcg tccgcgccgg cgagctccct cccgggctcc
     2221 ttgaccacgt cgagagggcg ggcatctccc tctttccgcg gcgatggtcc gagatgaagc
     2281 gctcctgcga ctgtcctgac tccggcgatc cctgcaagca cgaggcggcc gtctactatg
     2341 tcatcgcgca ggagatagac cgggatcctt ccgtgctgtt caggctccgc ggcttcgatc
     2401 ccgccgcctc ctccgcgccc ggcgagttgg cggggagggc cggcccggcg gccaagccgg
     2461 ccgctgatgg cgaggaaccg accgtcctcg agccgctcga gctccgcgag gcggatacct
     2521 ggacggcgcc cgatccggcc gactatccgg ccgagcttcc ttccctggcc tcctacgggg
     2581 ctctgctgcc ccgcatcctt ccgccaggcc ccgccctggc cggcctcgac ctggaagtcg
     2641 ccctcgccga attctaccac tgcctctccc gcgactggga gaagcccctc gccgcccggc
     2701 cgcccgcttc ctcgaagccg cgggctgcct tggacgagga ggcgggctgc gaggccgcgg
     2761 cccgctcctt cgccctggcc gggatccgcg tcgcggcgcg gggccagcgt ggcgagttca
     2821 tcctcccggg cggcgcgcgc ctcagccccc tcgaggcggc gcgccttgtc ctcgccatgg
     2881 acctcgagtc gggctcgcct tcctatgtct tcctgcgccg cttttccctg gccatgcgct
     2941 ccatcgtcgc cgcgggcgcc ttccacccgg atatccgcgc cggggacggc cgcttctccg
     3001 ccctgtggct gccggcgcgc ttcgcggccg acgcgcgcga agccctcgcc tgggtggagg
     3061 ccgcggccgt ggagcccgaa cggagcgcag gcccccgggc cgcccccgcc gccgggaccc
     3121 ggacttcggg gaaagccacc ctcgatcccc cctccctcgt cgcccttttc gcctccacct
     3181 tccttggcga ctacgtcgcc gccctggcct tccggccctc gggctccctc gcagcggcca
     3241 gtcccgcggc gaaagccctc ttcgggggcg aggaggtgga cgcgtccagc cccctcctcc
     3301 gctccctgcc gcgagccttg gccgcgcgct tctccgtcta ccgtctagcc gcccggcggt
     3361 cgggcttcga gctcgccgcg aagccggcgg cctcccgctc gggcgcgccg gcctaccgtc
     3421 tcaaggcgag cgtcctcgcg cccgagggga ggcgcctctc cctccgcgag gccggcgcca
     3481 tcctcggagc cgacgccctc gccttcccgg ccctcctc
//
LOCUS       DAOOSJ010000257         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17162_length_3518_cov_4.510540, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000257 DAOOSJ010000000
VERSION     DAOOSJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17162_length_3518_cov_4.510540"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLG14_10850"
     CDS             complement(<1..1067)
                     /locus_tag="PLG14_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOX49673.1"
                     /translation="MKFRIKYADRIVGLFVVLAAVILGASIVVLGANQRWFSKDYRFV
                     SRFGSGSGIAPGTAIYMKGFQVGKIKRIRLNADNEVDVDFTIYDTYYEKARVNSLLEL
                     VTSPIGLGSQLLFHPGRSEVRLEPGAFVPTADSAEGQGLIEQELVDIPPKDDTITRLL
                     ANVNPLLENVNKTVVTVNRTLTEVNRALAGQSSGPLGRIVVDAADTVATVDALVAEVK
                     DDAAGLVAKANELAASFGRISSNLEATTEALRDPTGLVPRLLDPKGSIKTILDDKNAL
                     YNSLIGSLAETQKTLRNIQSMTTTLNAQMPTVAATLEETKAAIIKAQDVLEGLKNNPL
                     LKGGIPERRDQTPLYQSFREG"
     gene            complement(1064..2005)
                     /locus_tag="PLG14_10855"
     CDS             complement(1064..2005)
                     /locus_tag="PLG14_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX49674.1"
                     /translation="MENAPVIELRGVTATRSGYEILKDISFSLADGETSVFMGGAGSG
                     KSSLLKTAAGVTIPDGGQVLFRGRDLAAMSRAEELEFHRLSGFVFQDAALWANQSLFD
                     NLALPCRFHDPRLSKAEVERAVRRAAEIVGYRDELSSRPAELSSGERRLIGLARALVL
                     DPELLFMDEPASYLDEESADRVYDIIAQLRARRRTIAVVSGSSDIAYRFADALGVLKE
                     GRLLAFGPYAEAVAWSDPSLRSATGRLRARKKAAEGDAALIEAWAEALASAPDPEPRG
                     SPPGPEPGGGAAGRAGDGSPGGGAGGRDGEAKDEEDA"
     gene            complement(2008..2787)
                     /locus_tag="PLG14_10860"
     CDS             complement(2008..2787)
                     /locus_tag="PLG14_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX49675.1"
                     /translation="MLERIGRALRERLRDLFYGLGFFANLVKESILFFRRRQVGFRVL
                     VMQILFTGYEALRISAVLAMAIGVAINLIGSSLLAGFGQGKLMYTLLIIVVTRELGPL
                     LTAFIVTARSGTAIATELGGMVVAHEIEAYVAVGVNPISYLAAPRFLGVVVSMLVLTV
                     YFNVFGLFGSFAVMQVLRPVGFQEYMRNLVEVLTVGDLLSGLAKSLIFGVIISTVSIH
                     RGFAVERASTEVPVAGIKAVGSSFVLIVLADAAVTAVQYLG"
     gene            complement(2863..>3518)
                     /locus_tag="PLG14_10865"
     CDS             complement(2863..>3518)
                     /locus_tag="PLG14_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49676.1"
                     /translation="GIQVQLVDTAALRGKSFDPGIVLDGLAPQPRSLVVNIDYAFGEQ
                     AEEIMRSLLLLFGRNVRSISVLGKAGALLGERGDLLVPTAFVEQANDAFLPLGPLPEG
                     RLGRLANALPGRAVREGPLLTVGGTLLQNRAMLQFYRRIWGCVGLEMEGIWYLRAILE
                     AEELGVLRRGSRLGFIYYVSDLPLAAGQRLSERLSPQEGVPPLYAATREALSGILGA"
BASE COUNT          530 a         1173 c         1287 g          528 t
ORIGIN      
        1 tccccctccc tgaagctctg gtagagcgga gtctggtccc tgcgctcggg tatgccgccc
       61 ttgaggaggg gattgttctt gagcccctcc agcacgtcct gggccttgat gatcgcggcc
      121 ttcgtctcct ccagggtcgc ggccacggtc ggcatctgcg cgttgagcgt cgtcgtcatg
      181 gactggatgt tgcgcagggt cttctgcgtc tccgccaggc tcccgatcag gctgttgtag
      241 agcgcgttct tgtcgtccag gatggtcttg atggagccct tcgggtcgag gagcctcggc
      301 acgaggcccg tcgggtcgcg cagggcctcg gtggtcgcct ccaggttgga ggagatccgc
      361 ccgaaggagg cggcgagctc gttcgccttg gcgacgaggc ccgccgcgtc gtccttcacc
      421 tcggcgacca gggcgtccac cgtcgcgacc gtgtcggcgg cgtcgacgac gatccggccg
      481 agcggccccg agctctggcc cgcaagggcg cggttcacct cggtgagggt ccggttcacc
      541 gtgacgacgg tcttgttcac gttctcgagg agggggttca cgttcgcgag gaggcgggtg
      601 atcgtgtcgt ccttgggcgg aatgtcgacg agctcctgct cgatgaggcc ctgcccctcg
      661 gccgagtcgg ccgtcgggac gaaggcgccc ggctcgagcc gcacctcgct cctgcccggg
      721 tggaagagga gctgggagcc caggcctatg ggcgaggtga cgagctcgag gagggagttg
      781 accctcgcct tctcgtagta ggtgtcgtag atcgtgaagt ccacgtcgac ctcgttgtcc
      841 gcgttgagcc tgatccgctt gatcttgccc acctggaagc ccttcatgta gatggccgtg
      901 ccgggggcga tgcccgagcc cgagccgaag cgggacacga agcggtagtc cttggagaac
      961 cagcgctggt tcgcccctag gacgacgatg ctcgcgccga ggatgacggc cgccaggacg
     1021 acgaagaggc ccacgatccg gtcggcgtac tttatcctga acttcatgcg tcctcctcgt
     1081 ccttcgcttc cccgtcccgg cccccggccc cgccgccggg gctcccgtcc ccggcccttc
     1141 ccgccgcgcc tccgcccggc tccggcccgg gaggcgatcc gcgcggctcc ggatccggcg
     1201 ccgacgcgag ggcctccgcc caggcctcga tgagggcggc gtcgccctcg gccgccttct
     1261 tcctggccct gagccgcccc gtcgcgctgc gcaaggaggg gtcgctccag gcgacggcct
     1321 ccgcgtaggg accgaaggcc agcagcctgc cctccttgag gacgcccagg gcgtcggcga
     1381 agcgataggc gatgtcggag ctgccggaga cgacggcgat ggtccgccgg cgcgcgcgca
     1441 gctgcgcgat gatgtcgtag acgcggtcgg ccgactcctc gtccaggtag gaggccggct
     1501 cgtccatgaa gaggagctcg gggtccagga ccagagcccg ggccaggccg atgaggcggc
     1561 gctcgccgga ggagagctcg gcgggccgcg aggagagctc gtcgcggtag ccgacgatct
     1621 ccgcggcgcg gcggacggcg cgctcgacct cggccttgga caggcgcggg tcgtggaagc
     1681 ggcagggcag ggccaggttg tcgaagaggc tctggttggc ccagagggcg gcgtcctgga
     1741 agacgaagcc ggagaggcgg tggaactcga gctcctcggc ccggctcatc gccgccaggt
     1801 ccctgccccg gaagaggacc tggcccccgt cggggatggt gacgccggcc gcggtcttga
     1861 ggaggctcga cttccccgag cccgcgcctc ccatgaagac gctcgtttcg ccgtcggcca
     1921 gggagaagga gatgtccttg aggatctcgt agcccgaccg cgtcgcggtg acgccccgaa
     1981 gctcgatgac cggcgcgttt tccatggcta gcccaggtac tgcacggccg tgaccgcggc
     2041 gtcggccagg acgatgagga cgaagctcga gccgacggcc ttgatgccgg ctacggggac
     2101 ctcggtgctg gcgcgctcca ccgcgaagcc gcggtggatc gacacggtcg agatgatgac
     2161 gccgaagatg aggctcttcg cgaggccgga gaggaggtcg cctaccgtga ggacctccac
     2221 caggttccgc atgtactcct ggaagccgac gggacgcagg acctgcatca ccgcgaagga
     2281 gccgaagagg ccgaagacgt tgaagtagac ggtgaggacg agcatggaga cgacgacgcc
     2341 caggaagcgg ggggcggcga ggtaggagat cgggttcacg ccgacggcga cgtaggcctc
     2401 gatctcgtgg gccacgacca tgccgccgag ctcggtcgcg atggccgtgc ccgagcgcgc
     2461 ggtgacgatg aaggcggtca ggagcgggcc gagctccctc gtgacgacga tgatgaggag
     2521 ggtgtacatg agcttgccct ggccgaagcc cgcgaggagg gaggagccga tcaggttgat
     2581 cgcgacgccg atggccatcg cgaggacggc ggagatgcgc agggcctcgt agcccgtgaa
     2641 gaggatctgc atgacgagga cgcggaagcc gacctggcgc cgccggaaga agaggatgga
     2701 ttccttcacc aggttcgcga agaacccgag accgtagaac aggtcccgga gccgctccct
     2761 gagcgcgcgg cctatccgct cgagcatgtg ccttgttccc ccgtcgcctg ccccgatcgc
     2821 ggcctgcgtc ccgttcaagt atcggacttc gccgcccggc tctcaagctc ccagtatgcc
     2881 ggaaagcgcc tcgcgcgtcg ccgcgtagag gggcgggacc ccctcctggg gggagaggcg
     2941 ctcggaaagc cgctggccgg cggccagggg caggtcgctc acgtagtaga tgaagccgag
     3001 ccggctcccc cgccgcagga ccccgagctc ctccgcctcc aggatggcgc gcaggtacca
     3061 gatcccctcc atctccaggc cgacgcagcc ccagatccgc cggtagaact ggagcatggc
     3121 gcggttctgg aggagggtgc cccccaccgt gaggaggggg ccctccctga ccgcccgccc
     3181 cggcagggcg ttggccaggc gcccgagcct cccctcgggc aggggaccca gggggaggaa
     3241 ggcgtcgttc gcctgctcga cgaaggccgt ggggacgagg aggtcgccgc gctccccgag
     3301 gagggctccc gccttcccca ggacggagat ggagcggacg ttccgcccga aaaggaggag
     3361 gaggctcctc atgatctcct ccgcctgctc gccgaaggcg tagtcgatgt tgacgaccag
     3421 gctcctcggc tgcggcgcga ggccgtccag gacgatgccc gggtcgaagg acttgcccct
     3481 gagggccgcc gtgtcgacga gctggacctg gatgcccg
//
LOCUS       DAOOSJ010000258         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17387_length_3490_cov_3.598253, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000258 DAOOSJ010000000
VERSION     DAOOSJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17387_length_3490_cov_3.598253"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PLG14_10870"
     CDS             <1..1413
                     /locus_tag="PLG14_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOX49677.1"
                     /translation="RGMVYDALLLNAQVPDLVLTLDEYLERSLGRLMEAIDARYASAR
                     RLLGAVVLAVSAAGALVVLAMLLGFIRFFAAALGGFGAAIGRWTDCDLSAAIDSSGHD
                     EISALARDLNAAMASFSRVIEGIKVISEKAGAVREEIVAAAEETSAAMTEIGANIGSI
                     RTRMDRMVEKMGESAAAAEAIGRNVGSLDKRLAEQSEALARSSSGAAAISAAVGEAGA
                     VAARRGEEAGRLEALAAEEQRRFGETNALIASTAEDVGRVREIVAIINAVASQTNLLA
                     MNAAIEAAHAGDAGRGFAVVADEIRKLAESTNRNAAIIKSTVGGMSTRIAEVQEAGAK
                     TDEAFRSIEERTRETRLSLDELGGLMRELDSSARAMAADAELGASGSREAKERSSEIL
                     ASAKDSLAAVEQVRSLGLEIRNGMSEIELGARDTGTAMLHVRDLSRESSDAVEELSRS
                     VASYKTAACAEPVPEATAEA"
     gene            1679..2557
                     /locus_tag="PLG14_10875"
     CDS             1679..2557
                     /locus_tag="PLG14_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOX49678.1"
                     /translation="MSGNDSMIFSLYGALSEDAAGGFSLKTLCAMLARLGLSAEASRL
                     ALSRMARRGFLCTKRRGKASFYFLSEAGAAVIDRAEKGSVCREGSPAWDGRFRLVSYE
                     LPESLREERAALAEALRRDGFGRAAAGLWAGPFEPGAALRERLGSPGLDGLVEVYEAV
                     HRGDDRAFARRVFRLDERRKALDAFLARFSADREAFLLRRASGEGTPDAACFTAYFEA
                     LDAYVDAMASVPPLPPELLPQDWPAEAAKELFVSYRALVREGAGAFYESVYEPFEPAL
                     GTRAKGPWPSRGGPAN"
     gene            2588..>3490
                     /locus_tag="PLG14_10880"
     CDS             2588..>3490
                     /locus_tag="PLG14_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOX49679.1"
                     /translation="MDKRIVARKTRAAALALALLSGAALAACSKAEPGAGRGRNAPPA
                     APAKWEAAKGSALLVVNMQKGLMPIVDGEKVAAAIGGLVEKAEAAGALVVWVHSRDGE
                     LREGAEGFGIAPPLAPSPSHLRLVKEAPSAFSGTELEELLDARKVGRVVVCGISSDGC
                     VNSTVQSAYCYGYRTVVASDAHSVHGPDASAAKIQAVNRSWAANPRLELLPSSKVEFS
                     PAAGAPGGAASDSATSGDPASGSAVSGSAGASSTVSGSAAAGGLGAEGWAHSPAFAAF
                     LDSSYRKLFARSPETATQLGLSAWA"
BASE COUNT          492 a         1290 c         1210 g          498 t
ORIGIN      
        1 cgcggcatgg tctacgacgc cctcctcctc aacgcgcagg tgcccgacct cgtcctcacc
       61 ctggacgagt acctcgagcg ctcgctcggc cgcctcatgg aggccatcga cgcccgctac
      121 gcctccgccc ggcgcctcct cggcgccgtc gtgctcgcgg tctcggcggc gggggccctg
      181 gtggtcctcg ccatgctcct aggcttcatc cgcttcttcg ccgcggccct gggcggcttc
      241 ggcgcggcca tcggccggtg gacggactgc gacctctccg cggccatcga ttcgagcggc
      301 catgacgaga tctccgccct ggcccgcgac ctcaacgccg cgatggcctc cttctcccgc
      361 gtcatagagg gcatcaaggt catctccgag aaggcgggag ccgtccggga ggagatcgtg
      421 gccgcggcgg aggagacgag cgccgccatg acggagatcg gcgcgaacat cggctcgatc
      481 cggacgcgga tggaccgcat ggtcgagaag atgggcgagt cggccgccgc cgccgaggcc
      541 atcgggcgga acgtcggctc cctggacaag cgcctagccg agcagagcga ggccctggcc
      601 cgctccagct cgggcgcggc ggcgatctcc gccgccgtag gcgaggccgg cgccgtcgcc
      661 gcccgcaggg gcgaggaggc ggggcgcctg gaggccctgg cggccgagga gcagcgccgc
      721 ttcggcgaga cgaacgccct catcgccagt acggcggagg acgtgggcag ggtgcgggag
      781 atcgtcgcca tcatcaacgc ggtcgcctcc cagaccaacc tcctcgcgat gaacgccgcc
      841 atcgaggcgg cccacgcggg cgacgccggc cgcggcttcg ccgtcgtggc tgacgagatc
      901 aggaagctcg cggagtccac gaacaggaac gccgccatca tcaagtcgac cgtcggcggc
      961 atgtccacgc gcatcgctga ggtccaggag gccggcgcca agaccgacga ggccttcagg
     1021 agcatcgagg agcgcacccg cgagacgcgg ctcagcctgg acgagctggg cggcctcatg
     1081 agggagctgg actcctcggc cagggccatg gccgccgacg cggagctcgg cgcctcgggc
     1141 tcccgcgagg cgaaggagcg ttcgagcgag atcctcgcga gcgccaagga ctccctggcc
     1201 gccgtcgagc aggtccggag cctgggactc gagatccgca acggcatgtc ggagatagag
     1261 ctcggggcca gggacacggg cacggcgatg ctccacgtcc gcgacctctc gcgcgagagc
     1321 tccgacgccg tggaggagct ctcccggagc gtcgcctcct acaagaccgc cgcctgcgcg
     1381 gagcccgtgc ccgaggcgac tgcggaggcc taggccggcc cggacgccct gtccggcctc
     1441 ggccgccgcg tccgccacgg ccgcctcgtc cgccacggcc gccgcggcca cgtcctaggc
     1501 tcccgccgcc gcggatagcc cgtagcgaag caagagcccg accctgaagt cctcgacacc
     1561 cttcggccgc tcaggacccg aggaaaaagt cgtgaagggg cttgcgtttt ctcttttttg
     1621 tgtcatattt ttgtgtatcg ctagtttaac gacacacata aacaggacac catagatcat
     1681 gtcaggcaac gattcgatga tcttctcgct ctacggcgcc ctctcggagg acgccgcggg
     1741 cggtttttcc ctcaagaccc tctgcgcgat gctcgcccgc ctcgggctct cggccgaggc
     1801 ttcgcggctc gccctctcgc ggatggcgag gaggggcttc ctttgcacga agcgccgggg
     1861 aaaggcgagc ttctatttcc tctccgaggc tggggctgcc gtcatcgacc gggccgagaa
     1921 ggggagcgtc tgccgggaag gctctccggc ctgggacggc cgcttccgcc tcgtctccta
     1981 cgagctaccg gagagtctcc gggaggagcg cgcggccctg gccgaggccc tgaggcggga
     2041 cggcttcggc agggcggcgg cgggtctctg ggcggggccc ttcgagccgg gcgcggccct
     2101 ccgcgagcgc ctcgggagcc ccggactcga cggcctcgtc gaagtgtacg aggcggtcca
     2161 tcgcggcgac gaccgggcct tcgcccggcg cgtcttccgg ctcgacgaaa ggcggaaggc
     2221 cctggacgcc ttcctcgcgc gcttctccgc ggaccgggaa gccttcctcc tgcgccgggc
     2281 ctccggcgag ggcacgcccg acgccgcctg cttcaccgct tatttcgagg ccctggacgc
     2341 ctacgtggac gcgatggcct cggtgccgcc ccttccgccc gagctccttc cccaggattg
     2401 gcccgcggag gccgccaagg agctcttcgt ctcctaccgg gcccttgtgc gcgagggcgc
     2461 gggcgccttc tatgaatcgg tctacgagcc cttcgagccc gccctcggga ccagggccaa
     2521 ggggccctgg ccgtcccgag gcgggcctgc gaactaggga ccttccgtcc cgccaaggag
     2581 gccttccatg gacaagagga tcgtcgcgcg caagacgagg gccgccgccc tcgccctggc
     2641 tcttctctcg ggagcggccc tggccgcctg ctcgaaggct gagccgggcg ccggcagggg
     2701 ccggaatgcg ccccccgccg ctccggccaa atgggaggcg gccaagggca gcgccctcct
     2761 cgtggtgaac atgcagaagg gcctcatgcc catcgtcgac ggggaaaaag tcgcggccgc
     2821 catcggcggc ctcgtcgaga aagccgaggc ggcgggagcc cttgtcgtat gggtccactc
     2881 gcgcgacggc gagctccgcg agggcgccga aggcttcggg atcgcccctc ccctcgcccc
     2941 ttcgccttcg catctgcggc tcgtgaagga ggcgcccagc gccttctccg gcacggagct
     3001 cgaggagctt ctcgacgcga ggaaggtcgg cagggtcgtc gtctgcggca tctcgagcga
     3061 cggctgcgtg aactcgaccg tccagtcagc ctactgctac ggctaccgga ccgtcgtggc
     3121 ctcggacgcc cactcggtcc atggcccgga cgcctcggcc gcgaagatcc aggccgtcaa
     3181 caggtcctgg gccgcgaatc cccgcctcga gctcctcccg tcctccaaag tcgagttctc
     3241 cccggcagcc ggggccccgg gcggcgcggc ctcagacagc gcgacctcag gcgaccccgc
     3301 ctcaggaagc gcagtctcgg gaagcgcagg cgcgtcaagc acggtttcgg gcagcgcggc
     3361 cgcgggcggc ctcggggccg agggctgggc ccactcgccg gccttcgctg cctttctcga
     3421 ctcctcctac cggaagctct tcgcccgctc ccccgagacg gcgacccagc tcggcctctc
     3481 cgcctgggcg
//
LOCUS       DAOOSJ010000259         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17501_length_3474_cov_4.588769, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000259 DAOOSJ010000000
VERSION     DAOOSJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17501_length_3474_cov_4.588769"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLG14_10885"
     CDS             <1..851
                     /locus_tag="PLG14_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOX49680.1"
                     /translation="AAIQGGILGGDVKDVLLLDVTPLSLGIETLGGVMTRLIPRNTTI
                     PCRKSQIFSTAADGQTAVSIHVLQGEREMAGQNRTLGRFDLMNIPPAPRGVPQIEVTF
                     DIDANGIVHVTAKDLGTGKEQKIRIEASSGLNDGDIDRMVREAEAHAADDKKEREKAE
                     IRNEADSLIYMTEKSLKDYGDKVSAADKATIEAGLADLKKAMEGQDLELMKQKTEALK
                     QASYKLAEEVYKSAGAAAQGAPGAGPEAGSGSRDSGSQGQQGPGSASGSADDVDYRVV
                     DDEEKK"
     gene            946..>2048
                     /gene="dnaJ"
                     /locus_tag="PLG14_10890"
     CDS             946..>2048
                     /gene="dnaJ"
                     /locus_tag="PLG14_10890"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOX49681.1"
                     /translation="MAKRDYYEVLGVQKDASKDDIKKAYRRLAIQNHPDKNPGDHAAE
                     ERFKEATEAYEVLADDQKRSAYDRFGFAGVEGMGGAGSAQDFSSVFKDFEDIFGFGDF
                     SNIFDSFFGGGGGRRGGGRERASRGSNLRYDLELSFEEAVFGKAVEISYSKHDACKSC
                     GGTGSADGSGRRVCPTCQGSGQVRRNSGFFSIAQPCPACRGEGSVIDKPCRDCEGSGL
                     AKKKQKIKVTIPPGVEDGKRVVIPGQGDVGPNGGQPGDLYVFIRVLPHEYFERDGNDL
                     YCALPVGMTTAALGGEVSFETIEKKTIKVTIPAGVQNGKMLRVREEGVPSASGRRGDL
                     YLKILVQIPQKLSKRGRELLEELRKAEGEDAAPR"
     assembly_gap    2049..2148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2149..3201
                     /locus_tag="PLG14_10895"
     CDS             <2149..3201
                     /locus_tag="PLG14_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX49682.1"
                     /translation="PDFASFNEALADEVHHSLYTRGRNPTVDLVEEKLAALEHGERAK
                     LVGAGVAAISHAVMAFVKSGDHVVCVEDCYSWTRTLLSGYLGRFGVSATFVEGRDPAE
                     IEAACRPNTRMIYLESPTTFTFKLQDLKAVAAIARRRGIKTVIDNTWATPIFCNPIDF
                     GIDLVVHSASKYLGGHSDLVAGVVVGSKADIDLIQKREFLQLGTVADPMMAWLLLRGM
                     RSLQVRMPAHYANALAVATALEAHPKVESVLYPMLDSYPDRELARRQMRGGSGLLSFR
                     LKTRVKAAVVRFTDELRLFKRAVSWGGYESLVFPDAVRYAGAVPEDRVGLVRIHVGLE
                     EKEAILGDLERALERA"
     gene            complement(3283..>3474)
                     /locus_tag="PLG14_10900"
     CDS             complement(3283..>3474)
                     /locus_tag="PLG14_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOX49683.1"
                     /translation="MLGTPILGPAAIIGLVTFVLCLFGCEFGRRIGAKFERWAETAGG
                     LVLIGIGIKILAEHLVKAI"
BASE COUNT          566 a         1211 c         1143 g          454 t
ORIGIN      
        1 gcgccgccat ccagggcggc atcctgggcg gcgacgtgaa ggacgtcctc ctcctcgacg
       61 tcacgcccct ctccctgggc atcgagaccc tgggcggcgt gatgacccgc ctcatcccca
      121 ggaacacgac gatcccctgc aggaagagcc agatcttctc caccgccgcc gacggccaga
      181 ccgccgtctc catccatgtc ctccagggcg agcgcgagat ggccggccag aaccgcaccc
      241 tgggccgctt cgacctcatg aacatcccgc cagcgccccg cggcgtgccc cagatcgagg
      301 tcaccttcga catcgacgcg aacggcatcg tccacgtcac cgcgaaggac ctcgggaccg
      361 gcaaggagca gaagatccgc atcgaggcct cctccggcct caacgacggc gacatcgacc
      421 ggatggtccg cgaggccgag gcccacgccg ccgatgacaa gaaggagcgc gagaaggcgg
      481 agatccgcaa cgaggcggac agcctgatct acatgaccga gaagtccctc aaggactacg
      541 gcgacaaggt ctccgccgcc gacaaggcca cgatcgaggc cggcctcgcc gacctcaaga
      601 aggcgatgga gggccaggac ctcgagctca tgaagcagaa gaccgaggcc ctgaagcagg
      661 cttcctacaa gctcgccgag gaggtctaca agagcgcggg cgccgcggcc cagggcgccc
      721 cgggcgccgg ccccgaggct ggctccggct cccgggactc cggaagccag ggccagcagg
      781 gccccggttc ggcctccggc tccgcggacg acgtggacta tcgcgtcgtc gacgacgaag
      841 agaagaagta gcattccctc cccgccgggg gagccgctcc cgggaaggcc ccgcaagggc
      901 cttcccttcg gcttttcccg gcgcgcggac gagcgaggat agaccgtggc gaagcgcgat
      961 tattacgagg tcctgggcgt ccagaaggac gcttccaagg acgacatcaa gaaggcgtac
     1021 aggcgcctcg ctatccagaa ccatccggac aagaatccgg gcgaccacgc ggccgaggag
     1081 cgcttcaagg aggcgaccga ggcctacgag gtcctggccg acgaccagaa gcggagcgcc
     1141 tacgaccgct tcggcttcgc cggcgtcgag ggcatgggcg gggccggctc ggcccaggac
     1201 ttctcctcgg tcttcaagga cttcgaggac atcttcggct tcggcgactt ctccaacatc
     1261 ttcgactcct tcttcggggg cggcggcggg cgccggggcg gcgggcgcga gcgcgcctcc
     1321 cgcggctcca acctccgcta cgacctggag ctctccttcg aggaggcggt cttcggcaag
     1381 gccgtcgaga tcagctactc gaagcacgac gcctgcaagt cctgcggcgg cacgggctcc
     1441 gccgacggct ccggccgccg cgtctgcccg acctgccagg gctcgggcca ggtgcgccgc
     1501 aactcgggct tcttctccat cgcccagccc tgcccggcct gccgcggcga gggctccgtc
     1561 atcgacaagc cctgccgcga ctgcgagggc tcgggcctcg ccaagaagaa gcagaagatc
     1621 aaggtgacga tcccccccgg ggtggaggac ggcaagcgcg tcgtcatccc cggccagggc
     1681 gacgtgggcc cgaacggcgg ccagcccggc gacctctacg tcttcatccg cgtccttccg
     1741 cacgagtact tcgagcgcga cggcaacgac ctctactgcg ccctgcccgt cggcatgacg
     1801 acggcggccc tgggcggcga ggtctccttc gagacgatcg agaagaagac gatcaaggtg
     1861 acgatccccg cgggcgtcca gaacggcaag atgctccgcg tccgcgagga gggcgtgccc
     1921 tcggcctcgg ggcggagggg cgacctctac ctcaagatcc tggtgcagat cccccagaag
     1981 ctttcgaagc gcggccgcga gctcctggag gagctcagga aggccgaggg cgaggatgcc
     2041 gccccccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cgacttcgcc
     2161 tccttcaacg aggccctggc ggacgaggtc caccactcgc tctacacgag gggccggaac
     2221 ccgaccgtgg acctcgtcga ggagaagctg gccgccctgg agcacgggga gcgggccaag
     2281 ctcgtcggcg ccggcgtcgc cgcgatctcc cacgccgtga tggccttcgt gaagtcgggc
     2341 gaccacgtgg tctgcgtcga ggactgctac tcctggaccc ggaccctcct ctcaggctac
     2401 ctggggcgct tcggcgtgtc cgccaccttc gtggagggcc gcgaccccgc ggagatcgag
     2461 gcggcctgcc gcccgaacac gcggatgatc tacctggaga gcccgaccac cttcaccttc
     2521 aagctccagg acctcaaggc ggtggcggcc atcgcgaggc ggcgggggat caagaccgtc
     2581 atcgacaata cctgggcgac gccgatcttc tgcaacccga tcgacttcgg catcgacctg
     2641 gtcgtccatt cggccagcaa gtacctgggg ggccacagcg acctggtcgc cggcgtcgtc
     2701 gtcggctcga aggccgacat cgacctcatc cagaagcggg aattcctcca gctcggcacg
     2761 gtggcggatc cgatgatggc ctggctcctc ctccgcggca tgcgcagcct gcaggtccgc
     2821 atgcccgcgc attacgccaa cgcgctcgcc gtcgccacgg ccctcgaggc gcatccgaag
     2881 gtggagagcg tcctctaccc gatgctcgac tcctacccgg accgcgagct cgcgcggcgc
     2941 cagatgcgcg gcggctcggg cctcctctcc ttccggctca agaccagggt caaggccgcc
     3001 gtggtccgct tcaccgacga gctcaggctc ttcaagcgcg cggtgagctg gggcggctac
     3061 gagagcctcg tcttccccga cgcggtgcgc tacgcgggcg ccgtgcccga ggaccgcgtg
     3121 ggcctggtgc ggatccacgt gggcctggag gagaaggagg ccatcctcgg ggacctggag
     3181 cgggcgctgg agagggcctg aggcccgcgg cgcgtaggcg gcgcctggcc gcggcgctct
     3241 tgcggcgcgc ctgaaggtct cgcggcgggc cgcggccttg ggctagatcg ccttcacgag
     3301 gtgctcggcc aggatcttga tcccgatgcc gatcagcacc aggccgcccg cggtctcggc
     3361 ccagcgctcg aacttcgcgc cgatgcgcct gccgaactcg cagccgaaga ggcagaggac
     3421 gaaggtgacg aggccgatga tcgcggcggg cccgaggatg ggcgtgccga gcat
//
LOCUS       DAOOSJ010000260         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17650_length_3455_cov_6.228529, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000260 DAOOSJ010000000
VERSION     DAOOSJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17650_length_3455_cov_6.228529"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLG14_10905"
     CDS             complement(<1..208)
                     /locus_tag="PLG14_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49684.1"
                     /translation="MLVALAQMNSVIGDFEGNRARILAFAAEAAGAPARTASGAAGKA
                     AAGPSARLAARPDLLVFPELALCGY"
     gene            336..1085
                     /locus_tag="PLG14_10910"
     CDS             336..1085
                     /locus_tag="PLG14_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49685.1"
                     /translation="MKKTILIAHPGEAPSADAPYGGPLAAALVAEARAAGWTVALALK
                     ADPRAAAAAPAAKPPKPAAPGGKATEPEAAPGGREEATVLAWNPASYVSACALALAAK
                     NALGEIEAAIVLSEPAALGADLLADGPGKAETAIGEATLGPALLCRELARRFEARKAG
                     ALLLLGEEPPEDAPLGPVAALAAGAFRGLGDGLFAASAEAPYDVYGVADRGGLPAEAA
                     RFALRLLEEGKSGKSGRWLRFGGKAGIFGVF"
     gene            1096..2196
                     /locus_tag="PLG14_10915"
     CDS             1096..2196
                     /locus_tag="PLG14_10915"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOX49686.1"
                     /translation="MAGKALSIGILTSGGDCPGLNAAIRGVAKAAMGSGGARIVGVEN
                     GYRGLIEGRARELGPAELSGILTRGGTILGASREKPFEDRGLPFRTDADEGMDKPELI
                     KENYRKLGLDCLVVLGGNGTQTTAYRLQEEGLSVVGLPKTIDNDLWGTDVSFGFHSAV
                     AVATEAIDRLHSTASSHDRVMVIELMGHKAGWLALYAGIAGGGDVVLLPELPYDPAAI
                     ASHLRRRAAEGKAFSIVVVAEGAMSVAESLMDKKERKRLRAASPFPSIGYRVAREIEE
                     ATGAEARVTVLGYLQRGGVPAPYDRLLATAFGAAAADLISARDYGRMVALRGGAVSSV
                     PLSEVAGKTRRVPEGHELLRTARSVGTCLGDE"
     gene            2199..2612
                     /locus_tag="PLG14_10920"
     CDS             2199..2612
                     /locus_tag="PLG14_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOX49687.1"
                     /translation="MGATLRLRVLPGRLSVCRLPPGAPFDPSALFSRAPATGAGPAIL
                     SVALSAEELSVVCAEGAEPAGARVESGWRTLKVQGPLDFGLTGVLAAIASPLAAAGIP
                     IFALSTFDTDYVLVKEPLLPAAAGALRGAGMDVEG"
     gene            2749..3402
                     /locus_tag="PLG14_10925"
     CDS             2749..3402
                     /locus_tag="PLG14_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOX49688.1"
                     /translation="MSLKTFILSELYGGGLAYGLELTRRFERARIVSWEGVTHGSVYR
                     ALARLREEGLVARYSRERAGGPTTRRGGGLRNRKFYAITPRGRRWFEAALSGELSEPR
                     TYRNPLNIAVHCLRALPAPRAVALLELRRRRLAGHEALAAEVARKARGRGRRDAYERL
                     AAERILMKARAELFFAGALSEYLKGATRIDERCGARREPASDARGHGGSAPIQGPQL"
BASE COUNT          439 a         1374 c         1217 g          425 t
ORIGIN      
        1 ggtagccgca gagggcgagc tcggggaaga cgaggaggtc ggggcgcgcc gctaggcggg
       61 ccgaggggcc ggccgcggcc ttgcccgccg cgccggacgc cgtccgggcc ggcgcgccgg
      121 ccgcctcggc cgcgaaggcg aggatgcggg cgcgatttcc ctcgaaatcg ccgatgacgg
      181 agttcatctg ggcgagggct acgagcatgg gaacctccgg gaggggctca ggacgcttgg
      241 ggcccggcct tccccgcctt gggggcccgg atccgggtcg ccggagacgg gggccggcct
      301 tgcgatcggc cacccgcgat agtacactcg gccccgtgaa gaagacaatc ctgatagccc
      361 atcccggcga ggcgcccagc gccgacgccc cctacggcgg ccccctcgcc gccgccctcg
      421 tggccgaggc ccgcgccgcg ggctggaccg tcgccctcgc cctcaaggcc gacccccggg
      481 cggccgccgc ggcccccgcc gccaagccgc ccaagccggc ggccccgggc gggaaggcga
      541 cggaacccga ggccgccccg ggcggccgcg aagaggcgac cgtcctcgcc tggaatcccg
      601 cctcctacgt ctccgcctgc gccctcgccc tcgcggcgaa gaacgccctg ggcgagatcg
      661 aggcggcgat cgtcctctcg gagccggcgg ccctgggcgc cgacctcctc gccgacgggc
      721 ccggcaaggc cgagacggcc ataggcgagg cgaccctggg gccagccctc ctctgccgcg
      781 agctcgcgcg ccgcttcgag gcgcggaagg ccggcgccct cctcctcctc ggagaagagc
      841 cgccggagga cgcgcccctc ggccccgtcg ccgccctcgc ggccggcgcc ttccgcggcc
      901 tgggcgatgg cctcttcgcc gcctcggcgg aggcgcccta cgacgtctac ggcgtcgcgg
      961 accgcggcgg gcttcccgcg gaggccgcgc gcttcgccct gcgcctcctg gaggagggca
     1021 agtcgggcaa gtccggacgc tggctccgct tcggcggcaa ggccggcatc ttcggcgtct
     1081 tctaggaggc tggccatggc cggtaaagcg ctttccatcg gcatcctgac gagcggaggc
     1141 gactgtcccg gcctcaacgc cgcgatccgc ggcgtcgcca aggcggcgat gggctcgggc
     1201 ggggcgagga tcgtcggcgt cgagaacggc taccgcggcc tcatcgaggg ccgggcccgg
     1261 gagctcgggc cagcggagct ctccggcatc ctcacccgcg gcggcaccat cctgggcgcc
     1321 tcgcgggaga agcccttcga ggaccgcggc ctccccttcc ggaccgacgc cgacgagggc
     1381 atggacaagc ccgagctcat caaggagaac taccggaagc tgggcctcga ctgcctcgtg
     1441 gtcctgggcg gcaacggcac ccagaccacc gcctaccgcc tccaggagga gggcctctcc
     1501 gtcgtcggcc tccccaagac catcgacaac gacctctggg gcaccgacgt ctccttcggc
     1561 ttccactccg ccgtcgccgt cgcgacggag gccatcgacc gcctgcactc gaccgcgagc
     1621 tcccacgacc gcgtcatggt gatcgagctc atgggccaca aggccggctg gctcgccctc
     1681 tacgcgggca tcgccggggg cggcgacgtc gtcctcctcc ccgagctccc ctacgacccc
     1741 gccgcgatag cctcccacct ccgccgcagg gcggccgagg gcaaggcctt ctccatcgtc
     1801 gtcgtcgcgg agggcgcgat gagcgtcgcc gagtcgctca tggacaagaa ggagaggaag
     1861 cgcctccgcg ccgcctcgcc cttcccctcg atcggctatc gcgtcgccag ggagatcgag
     1921 gaggcgacgg gcgcggaggc ccgcgtcacg gtcctcggct acctccagcg gggcggcgtc
     1981 cccgccccct acgaccgcct cctcgccacg gccttcgggg ccgccgccgc cgacctcatc
     2041 tcggcccgcg actatggccg catggtcgcc ctgcgcggcg gcgccgtctc ctccgtcccc
     2101 ctctccgagg tcgcgggcaa gacccgccgc gtccccgaag gccacgagct cctccgcacc
     2161 gcgcggagcg tcggcacctg cctcggggac gagtagggat gggcgccacc ctccgcctcc
     2221 gcgtcctccc cggccgcctc tccgtctgcc gcctcccgcc cggggctccc ttcgacccgt
     2281 ccgccctctt ctcccgcgcc ccggccaccg gggccgggcc ggccatcctc tccgtcgccc
     2341 tgagcgcgga ggagctctcc gtcgtctgcg ccgagggcgc cgagcccgcc ggggcccgcg
     2401 tcgagtccgg ctggaggacc ctcaaggtcc agggccccct cgacttcggc ctgaccggcg
     2461 tcctcgccgc gatcgcctcc cccctcgcgg cggccggaat ccccatcttc gccctctcca
     2521 ccttcgacac cgactacgtc ctcgtgaagg agcccctgct ccccgccgcg gccggggcct
     2581 tgaggggggc gggcatggac gtggaaggct aggaggcggc cggctgacga cccagttgcg
     2641 cgtatgcaca actggtcttg cggaccggtc ggacgcggac taggctttca ggcagagcga
     2701 aactgcgcgc cggcccagca gcaccgtacc cggcgccgga gatccccgat gtccttgaag
     2761 accttcatcc tctcggagct ctacgggggc ggcctcgcct acggcctgga gctgacccgg
     2821 cgcttcgagc gcgcccggat cgtctcctgg gagggcgtca cccacggcag cgtgtaccgg
     2881 gccctggccc gcctccgcga ggagggcctg gtcgcgcgct attcccgcga gcgggcaggc
     2941 ggtccgacga ccaggcgcgg aggaggcctc aggaaccgca agttctacgc catcaccccg
     3001 cgcggcaggc gctggttcga ggccgcgctc tccggcgagc tctcggagcc gcggacctac
     3061 cgcaaccccc tgaacatcgc cgtgcactgc ctccgcgccc tgccggcccc gcgcgcggtc
     3121 gccctcctcg agctccgcag gcggaggctc gctggccacg aggccctcgc ggccgaggtg
     3181 gcgcgcaagg cccgcggccg gggcaggcgg gacgcctacg aacgcctggc cgcggagcgc
     3241 atcctcatga aggccagggc cgaactcttc ttcgccggcg ccctctcgga gtacttgaag
     3301 ggcgcaacgc gaatcgatga gcgttgcggg gcgcggcgcg agccggcgag cgacgcgagg
     3361 gggcatgggg gatctgcccc catccaggga ccgcagctgt agcgtggggg tagcggagaa
     3421 gcccggagcc cgcgccacga ggcgcgggcg aggac
//
LOCUS       DAOOSJ010000261         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17654_length_3455_cov_3.005294, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000261 DAOOSJ010000000
VERSION     DAOOSJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17654_length_3455_cov_3.005294"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="dxr"
                     /locus_tag="PLG14_10930"
     CDS             <1..896
                     /gene="dxr"
                     /locus_tag="PLG14_10930"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOX49689.1"
                     /translation="RETEAELVVNGAAGAAGLAPSLAALESGKDLALANKESVVMGWG
                     LLKAAAARHGRAILPVDSEHAALFQLINRVGAAEVDELVVTASGGAFRDRPLAELGAV
                     RPDEAAAHPNWSMGRKISIDSATMANKGLEVIEASRLFGVEPGRVKVLIHPQSLVHAL
                     VRARDGSLYANASEPDMRLPIMNALAWPEALACPFGRLELAGRVLEFRAPDPERYPLL
                     GLAYAALAAGEGATVAYNAADEAAVAAYESGALGFLDIARVVERTLSASWPGSLPSLD
                     EVLEADRAARREAAAAIKELR"
     gene            893..2260
                     /locus_tag="PLG14_10935"
     CDS             893..2260
                     /locus_tag="PLG14_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOX49690.1"
                     /translation="MILTILLGLAGLGLVVLVHELGHFAAARAVGVEVEAFSIGWGPR
                     LFGWKRGATEWRVSAFPIGGYCRMKGEEDFRKAVAEKSETIPREPGTFYGASPWKRIA
                     IALSGPVANVVFAAVVFVAVSAVGYTVRTSGNRIVLASEFSLDGKPAPTGLPADAGGL
                     KSGDRVVAANGKPVRDYADLQEVIALNPEERLDFAVERDGARLDLAVTPRMDKSSGAG
                     RIGIYAWTEPLVEGVVAGSAADIAGVKEGDRVVSVGGEPVRHAIELLSRLSARPERVV
                     LGLDRRGESVEARLVLSYEGGGSNLGLSFATLPHTVKSESLGLAFSDGLRETWKTFSI
                     SVQGLGLLFRGVDVFKAISGPARITWLVGSTASEGMAESGANGLVVAFNFLGFLSIGL
                     FIMNLLPIPALDGGMILMFAIEGARRKPLRTKTVYRFQFIGMAFILALFVLATVSDLI
                     FFAGK"
     gene            2276..2899
                     /locus_tag="PLG14_10940"
     CDS             2276..2899
                     /locus_tag="PLG14_10940"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOX49691.1"
                     /translation="MRKVVCMCENAFEADLPERVDLDAEPGRLDEILAGAFFKVACPS
                     CGAVLKPELPVRLASRKRGLEVQVLPELDRLAFYLDRVQAPKEAEVLVGYAELYERAR
                     IVADGLDPLAVEIVKYYLALKAEEKAAEGAAVLISYAGREGASGAEKLLFHVSGIKEG
                     EVAVLHVGLDYYERTLAEKRKTLQSEPFDKLFAGRYKSIRALEAEAE"
     gene            3130..>3455
                     /locus_tag="PLG14_10945"
     CDS             3130..>3455
                     /locus_tag="PLG14_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49692.1"
                     /translation="MRGKNAFTLAGVAFAALAIAMPAIFVTACSDALLGAVSRDVEQA
                     LANDPTIEILRGSTPIVDGSVVDWGNNPRDSTIDVSFQIKNTGTGNLLLETPAVVPEL
                     VSDGFSL"
BASE COUNT          516 a         1290 c         1092 g          557 t
ORIGIN      
        1 tccgcgagac ggaggccgag ctcgtcgtga acggagcggc cggcgccgcg ggccttgccc
       61 cctccctggc ggccctcgag tccggcaagg acctcgcgct cgcgaacaag gagagcgtcg
      121 tgatgggctg gggcctcctc aaggccgcgg ccgcccgcca cggccgggcc atcctccccg
      181 tcgactccga gcacgccgcc ctcttccagc tcatcaaccg cgtcggcgcc gcggaggtcg
      241 acgagctcgt cgtcaccgcc tcgggcggcg ccttccgcga ccgccccctt gccgagctcg
      301 gcgccgtcag gcccgacgag gccgcggccc accccaactg gagcatgggc cggaagatca
      361 gcatcgactc cgcgacgatg gcgaacaagg gcctggaggt catcgaggcc tcgcggctct
      421 tcggcgtcga gcccggccgc gtgaaggtcc tcatccaccc ccagagcctg gtccacgccc
      481 tggtccgggc ccgggacggc tccctctacg ccaacgcctc ggagccggac atgcggctcc
      541 ccatcatgaa cgccctggcc tggcccgagg ccctggcctg ccccttcggc aggctggagc
      601 tcgcgggccg cgtcctcgag ttccgggcgc ccgatcccga gcgctatccc ctcctcggcc
      661 tcgcctacgc ggcgctggcc gcgggcgagg gcgcgacggt cgcctacaac gccgcggacg
      721 aggccgccgt cgcggcctac gagtcgggag ccctgggctt cctcgacata gcccgcgtcg
      781 tggagcggac cctctccgca tcctggcccg ggagcctccc ctcgctcgac gaggtgctgg
      841 aggccgacag ggcggcccgc cgcgaggcgg cggccgccat caaggagctt cgatgatcct
      901 cacgatcctg ctcggcctgg ccggcctcgg ccttgtcgtc ctcgtccacg agctcggcca
      961 tttcgccgcg gcccgcgccg tcggcgtcga ggtggaggct ttctccatcg gctggggccc
     1021 ccgcctcttc ggctggaagc gcggcgcgac cgagtggcgc gtctcggcct tccccatcgg
     1081 cggctactgc cgcatgaagg gcgaggagga cttcaggaag gccgtcgccg aaaagagcga
     1141 gaccatcccc cgcgagccgg ggaccttcta cggcgcctcg ccctggaagc gcatcgccat
     1201 cgccctctcc ggccccgtcg ccaacgtcgt cttcgccgcc gtcgtcttcg tcgccgtctc
     1261 ggccgtcggc tacacggtca ggacctccgg caaccggatc gtcctggcga gcgagttcag
     1321 cctggacggg aagcccgcgc cgacgggcct gcccgccgac gcaggcggcc tcaagagcgg
     1381 cgaccgcgtc gtcgccgcga acggcaagcc cgtccgcgac tacgccgacc tccaggaggt
     1441 catcgccctc aatcccgagg agcgcctgga cttcgccgtg gagcgcgacg gcgcccgtct
     1501 cgacctggcc gtgacgccca ggatggacaa gagctcgggc gccgggcgga tcggcatcta
     1561 cgcctggacc gagcccctcg tggagggcgt cgtagccggc agcgccgccg acatcgccgg
     1621 cgtcaaggag ggcgaccgcg tcgtctccgt cggcggcgag cccgtccgcc acgcgatcga
     1681 gctcctctcg cgcctctccg cccgcccgga gcgcgtcgtc ctgggcctgg accgccgcgg
     1741 cgagagcgtc gaggcgaggc tcgtcctctc ctacgagggg ggcggctcca acctcggcct
     1801 ttccttcgcc accctgcccc acaccgtgaa gtcggagagc ctgggcctcg ccttctccga
     1861 cggcctccgc gagacctgga agaccttctc catctccgtc cagggcctcg gcctcctctt
     1921 ccgcggcgtg gacgtcttca aggccatctc cggccccgcc cgcatcacct ggctcgtcgg
     1981 ctccaccgcc tccgaaggca tggccgagag cggagccaac ggcctggtcg tggccttcaa
     2041 cttcctcggc ttcctctcca tcggcctctt catcatgaac ctcctcccca tccccgcgct
     2101 ggacggaggc atgatcctca tgttcgccat cgagggggcg aggcggaagc ccctgcgcac
     2161 caagacggtc taccgcttcc agttcatcgg catggccttc atcctggcgc tcttcgtcct
     2221 ggcgacggtg agcgacctca tcttcttcgc cggcaagtaa aagaggagga caagcgtgcg
     2281 aaaagtcgtc tgcatgtgcg agaacgcgtt cgaggccgac ctgcccgagc gcgtcgacct
     2341 ggacgccgag cccggccgcc tggacgagat cctggccggc gccttcttca aggtcgcctg
     2401 cccgagctgc ggggccgtcc tcaagcccga gcttcccgtc cgcctggcct cccgcaagcg
     2461 cggcctggaa gtccaggtcc tgcccgagct cgaccgcctg gccttctacc tggatcgcgt
     2521 ccaggccccc aaggaagccg aagtcctcgt gggctacgcc gagctctacg agcgggccag
     2581 gatcgtagcc gacggcctgg accccctggc cgtggagatc gtgaagtact acctggccct
     2641 caaggccgag gagaaggccg cggagggcgc ggccgtcctc atctcctacg ccgggcgcga
     2701 gggcgcaagc ggggccgaga agctcctctt ccacgtctcc ggcatcaagg aaggcgaagt
     2761 cgccgtcctc cacgtcggcc tggactacta cgagcgcacc ctcgccgaaa agcgtaagac
     2821 cctccagtcc gagcccttcg acaagctctt cgccgggcgg tacaagtcga tcagggcctt
     2881 ggaggccgag gcggagtagg ctggttcccc ggttgcacgc ggaacgggct ccttctcagg
     2941 agcccgttct gtttatagcg aatctcccaa aagtcgatcc atcaggccct tgaatcagac
     3001 aatccgccgt tttttcaagt tcacgcttta aaaagtgacg aaagcgatat agtttgtctg
     3061 tacgttcgca atcgcctctt ttagcggacg aggctcgttt cgccatacct ttatcacagg
     3121 aagtgcagca tgcggggaaa aaacgcgttc acgctcgcag gcgtggcatt cgccgccttg
     3181 gcaatcgcga tgccggccat cttcgtcacg gcttgctccg acgctctgct cggggcggtt
     3241 tccagggacg tcgagcaggc gctggccaac gatccgacca tcgaaatact ccgtggatcg
     3301 actccgatcg tcgatggctc ggtcgtcgat tggggaaaca atccccgcga ctcgacgatc
     3361 gatgtttcct tccagatcaa gaacaccgga actggaaacc tccttcttga gacccccgcg
     3421 gtggttcccg agcttgtatc ggacggattc agcct
//
LOCUS       DAOOSJ010000262         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17685_length_3450_cov_6.622975, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000262 DAOOSJ010000000
VERSION     DAOOSJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17685_length_3450_cov_6.622975"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLG14_10950"
     CDS             complement(<1..427)
                     /locus_tag="PLG14_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX49693.1"
                     /translation="MAKPPNPELADRIVQLALAELADKPPEAVNMRSLAAKAGVSSTA
                     IYYYFASKEALFERIKFETLGELEATIGRVLEGAAGASGRDKLAALCRAFVAFSLSRP
                     HLAGLLMEELPPRLDLDEEAMERYYRIHDQARSLVVEALA"
     gene            complement(534..1718)
                     /locus_tag="PLG14_10955"
     CDS             complement(534..1718)
                     /locus_tag="PLG14_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOX49694.1"
                     /translation="MSASAFPLARVRAILPLLSGHRRALHAMPELGLELPRTAAYVEA
                     ELLRLGLKPRRSGDGMWADVGTRGPLVALRADMDALPIEEATGAAYASRTPGLMHACG
                     HDAHAAALLGAAALLAEAAPGLPYRVRLVFQTGEEGQAGALPLIEAGVLEGVAAIAAG
                     HAGDLSDELAPGQAGFLPGPMMAASDRFRGSFVGSGGHGAAPHKSPDPIVAFAEYALA
                     LQALRAREIEQTRPAVVSLCQVSSGSTYNVIPERLGFMGTARSLDTPTRAFLEQRIGE
                     LGKAVAAMRGLSFEFEWLGGYPPLLNEPEAAALAEEAARTVLGGERVRRLTRPSMGGE
                     DFAYYLEKVPGCFWFLNTQAPERGISFPNHNPRFDLDEGLLPELCAVNLAIAESLAAA
                     FG"
     gene            complement(1715..>1949)
                     /locus_tag="PLG14_10960"
     CDS             complement(1715..>1949)
                     /locus_tag="PLG14_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOX49695.1"
                     /translation="GESMAIVFASAFQDEALVVKSLLESAGIAAEVLAEGMLDATPLF
                     TTAKTGARVAVPDEDEADAAALVADYRANKGKA"
     assembly_gap    1950..2049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2050..3350)
                     /locus_tag="PLG14_10965"
     CDS             complement(<2050..3350)
                     /locus_tag="PLG14_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOX49696.1"
                     /translation="MFEAAIYRKRRAALVAGLARRGAGEGLALFLGNGESPMNYADNT
                     YPFRQDSSFLYFFGPSRPGLAGTIDLATGRSAIFADDASMDDIVWTGPQVSAAELAAL
                     AGADEALPRGALAARARAAGPAGLLYLPPYREETRRELAELLGTTAAEADAGASLPLV
                     QAALELRELKGPEEVAELEAAVATTVRMHRAALDLARPGMRESEVAATVTGIALAANG
                     ALSFPVIATTRGATLHNHDHGRVLEEGGLFLLDCGAETAGGYAGDLTTTFPIGKRFDQ
                     RQRAVYEIVLSMGRAATAILRPGLPFKEAHAAAARAAVEGLSALGLMRGDPAEAAEKG
                     AHALFFPHGLGHQIGLDVHDMESYGEDWVGYDGAGRRKDFGWKSLRLGKPLKAGMVHS
                     VEPGLYFIPELFARWKAEGKFPEHIDYRAVEAWLGLGGVRN"
BASE COUNT          452 a         1116 c         1308 g          474 t
ORIGIN      
        1 cggccagggc ctccacgacg aggctccggg cctggtcatg gatccggtag tagcgctcca
       61 tggcttcctc gtccaggtcg agcctgggag ggagctcctc catgaggagc cccgcgagat
      121 ggggccgcga gaggctgaag gcgacgaagg cccggcagag ggccgcgagc ttgtccctgc
      181 ccgaggcgcc ggccgcccct tccagcaccc ggcctatcgt ggcctcgagc tcccccaggg
      241 tctcgaactt gatccgctcg aagagggctt ccttcgaggc gaagtagtag tagatggccg
      301 tcgagcttac gccggccttc gcggcgaggc tgcgcatgtt gacggcctcc ggcggtttgt
      361 cggcgagttc ggccagcgcg agctggacga tgcggtcggc gagttcggga ttgggcggct
      421 tggccatcga agctccttgt cggtgttaag ataacaagga taacataaca atgtcaagtc
      481 attgggcagg ccgatgcgcg aaaagctccc cgggcgggga gcttttccgg tctttagccg
      541 aaggcggcgg ccagggactc ggctatcgcc aagttgacgg cgcagagctc ggggaggagg
      601 ccctcgtcca ggtcgaaacg gggattgtgg ttcgggaagg agatgccccg ctccggggcc
      661 tgggtgttga ggaaccagaa gcagcccggc accttctcaa ggtagtaggc gaagtcctcg
      721 ccccccatgg agggcctcgt gagccgcctc accctctccc cgcccaggac cgtgcgggcg
      781 gcctcctccg cgagggccgc tgcctcaggc tcgttcagga ggggagggta gccgcccagc
      841 cactcgaact cgaaggagag gccccgcatc gccgcgacgg ccttccccag ctcgccgatc
      901 cgctgctcca ggaaggcccg ggtcggggtg tcgaggctcc gcgccgtgcc catgaagccg
      961 aggcgctcgg ggattacgtt gtaggtcgag cccgaggaca cctggcagag ggagacgacg
     1021 gcgggcctcg tctgctcgat ctcgcgggcg cggagggcct ggagggccag ggcgtactcg
     1081 gcgaaggcga cgatgggatc ggggctcttg tgtggtgcgg cgccgtggcc gcccgagccg
     1141 acgaaggagc cgcggaagcg gtcggaggcc gccatcatcg ggccggggag gaagccggcc
     1201 tggccggggg cgagctcgtc ggagaggtcg cctgcgtgcc ccgccgcgat ggcggcgacg
     1261 ccctcgagga cgccggcctc gatgaggggc agggcgccgg cctggccctc ctctcccgtc
     1321 tggaagacga ggcggacgcg gtagggcagg ccgggcgccg cctcggcgag gagggccgcc
     1381 gccccgagga gggcggccgc gtgggcgtcg tggccgcagg cgtgcatgag gcccggcgtc
     1441 ctggaggcgt aggccgcgcc cgtcgcctcc tctatgggga gggcgtccat gtcggcgcgg
     1501 agcgcgacga gggggcccct cgtcccgacg tcggcccaca tcccgtcgcc cgagcggcgc
     1561 ggcttcaggc ccaggcggag gagctcggcc tcgacgtagg cggccgtccg gggcagctcg
     1621 aggccgagtt cgggcatcgc gtggagggcg cggcggtgcc cggagaggag ggggaggatg
     1681 gcgcggacgc gcgcgagggg aaaggcggac gcgctcacgc cttccccttg ttcgcccggt
     1741 agtcggcgac gagggcggcg gcgtcagcct cgtcctcgtc ggggacggcg acgcgggcgc
     1801 cggtcttggc cgtcgtgaag aggggcgtcg cgtccagcat gccctcggcc aggacttcgg
     1861 cggctatgcc cgccgactcg agaagggact tcacgacaag ggcctcgtcc tggaaggcgg
     1921 aggcgaatac gatggccatg ctctctcctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnt cgttcctcac gccgcccagg cccagccagg cctcgacggc gcggtagtcg
     2101 atgtgctcgg ggaacttgcc ctccgccttc cagcgcgcga agagttcggg gatgaagtag
     2161 aggccgggtt cgacggagtg gaccatgccc gccttgaggg gcttgcccag gcgtagggac
     2221 ttccagccga agtccttccg ccttcccgcc ccgtcgtagc cgacccagtc ctcgccgtag
     2281 ctctccatgt cgtggacgtc caggcctatc tggtggccca ggccgtgggg gaagaagagg
     2341 gcgtgggcgc ccttctccgc cgcctccgcg gggtcgccgc gcatgaggcc cagggccgag
     2401 aggccctcca cggccgcccg ggccgcggcc gcgtgggcct ccttgaaggg gaggccgggt
     2461 cggaggatgg cggtcgccgc gcggcccatg gagaggacga tctcgtagac ggcgcgctgg
     2521 cgctggtcaa agcgcttccc gatcgggaag gtcgtcgtga ggtcgcccgc gtagcccccc
     2581 gcggtctccg cgccgcagtc gaggaggaag aggccgccct cctcgaggac gcggccgtgg
     2641 tcgtggttgt ggagggtggc gccgcgggtc gtcgctatca cggggaagct cagggctccg
     2701 ttcgccgcca gggctatccc ggtgacggtc gccgcgacct cggactcccg catcccgggg
     2761 cgggccaggt ccagggcggc gcggtgcatc cgcacggtgg tcgcgacggc ggcctcgagc
     2821 tcggccactt cctctggccc cttcagctcc cggagctcga gggctgcctg gacgaggggg
     2881 agagaggcgc cggcgtcggc ctcggcggcc gtcgtaccga ggagctcggc cagctcgcgc
     2941 ctcgtctcct cgcggtaggg cgggaggtag aggaggccgg ccggcccggc ggccctcgcg
     3001 cgggccgcga gggcgcccct gggaagggcc tcgtccgcgc cggccagggc cgcgagttcc
     3061 gccgcggaga cctggggtcc cgtccagacg atgtcgtcca tggaggcgtc gtcggcgaag
     3121 atggcggagc gtcccgtcgc gaggtcgatg gtccccgcga ggcccggccg cgaggggccg
     3181 aagaagtaga ggaagctcga gtcctggcgg aagggatagg tgttgtccgc gtagttcatc
     3241 gggctttcgc cgttgccgag gaagagggcg aggccttcgc ccgcgccgcg ccgcgcgagg
     3301 cccgcgacca aggcggcccg gcgctttctg tagatggctg cttcgaacat gccccatgct
     3361 aggccgggcc tgccgcctgc gtcaacgcaa ttcgttcgcg gcccgcgcgg ctccccgagg
     3421 gccggagcgg atccccgagg gcccgcgcgg
//
LOCUS       DAOOSJ010000263         3420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_17921_length_3420_cov_5.363150, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000263 DAOOSJ010000000
VERSION     DAOOSJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17921_length_3420_cov_5.363150"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLG14_10970"
     CDS             <1..213
                     /locus_tag="PLG14_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49697.1"
                     /translation="KSAETRTVYVGVSVMTLALGLCSTIQGLFAAVILESSPPFGKCA
                     VYASFSSVYALILVAFLFAPVICRNS"
     gene            complement(264..701)
                     /locus_tag="PLG14_10975"
     CDS             complement(264..701)
                     /locus_tag="PLG14_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49698.1"
                     /translation="MDETTRLKTLQLFYAGLVVDAARQYAKQGVLERVTEDKKAEQAA
                     AAPAQLARLGIGTPEGLFATFAEIFGCADWKVEREGGLVRARASNCLACALAKRLGAP
                     SPCAISCVNPFQGLAAALPDPRELRVAETLWEGKACVFELSPA"
     gene            781..1584
                     /locus_tag="PLG14_10980"
     CDS             781..1584
                     /locus_tag="PLG14_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOX49699.1"
                     /translation="MIIRAGRRDRRPGLDVEGWRREYERVLSRARLRGEDCDEAVLAR
                     HLPFLGRLDAVEGSSIALFDFRTRSYRFLTASFRFLGGYPVLEAQAEGPDYFFRVMHG
                     PDLPFVLETTVRTFEFLESIPPAERKDWKLSFDFRIRHASGRLVRLLQQVLVLELDAR
                     GNIWLVLIANDLAPKGPGDESPSRRLENLKTGERRLFRGGEEEGKGLSRRELEILGLV
                     AEGLPSREIAERLFISVATVNNHRRRILEKLDTRSSAEAVRWAAARGLV"
     gene            1759..2652
                     /locus_tag="PLG14_10985"
     CDS             1759..2652
                     /locus_tag="PLG14_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX49700.1"
                     /translation="MIIDGHGHACGPYLSSDSIDRHLDEAGADLVVLTAGEAGSSKTY
                     AIKDDTERRPDDDYMGRNNRLIKVFVALSRAARRIRAGNESVHGLAKANPRVLQYYWA
                     SGESVEELEGLYGRMRFRGLKLQQVWAREDLGGRRFDELEEWLAAKGLPLFIHLADRK
                     DALALAARARRGLRTSVVVAHLYGFEALEEEGVLGAERLLFDVSNAYFVSRPRLERAV
                     ARVGAGRLCLGSDTPYGEDALRRTIARIDSLALPDGEKAKILGQNLAELLRVDGAAAR
                     RRASARPPAGAPAETGRGEDA"
     gene            2645..>3420
                     /locus_tag="PLG14_10990"
     CDS             2645..>3420
                     /locus_tag="PLG14_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49701.1"
                     /translation="MPRGAEASRRLEAAERLVARAEAPILSAAVVGSVSTGFADRDSD
                     IELELFVEELPTEAEREDLAARLGGLRVVHYPAPLRDGSVWTVCDSPEGWLELGWQVE
                     EKAIAALGELRRGAVLDHELLVLASAYGQAAWLRRSEAMGAAIVGLGAPSEELRAAIA
                     AEVLRQWDNELAWKVRATLVRRGDRLPLAQRHVADAQRMLRILFALNGRWEGDYKWIK
                     EQAEGLPRKPSDFAARLDLVLAAPDGVSANAALAGLLDELL"
BASE COUNT          469 a         1254 c         1213 g          484 t
ORIGIN      
        1 aagtccgcgg aaacgagaac cgtctatgtc ggcgtctcgg tgatgaccct ggcgctcggg
       61 ctctgctcca cgatccaggg cctcttcgcg gccgtcatcc tcgagtcctc gccgcccttc
      121 gggaagtgcg ccgtctacgc gtccttctcc tccgtctacg ccctcatcct cgtcgccttc
      181 ctcttcgcgc cggtgatctg ccgcaactcc tgaaaacggc cgccgggccg cggggcctcc
      241 gcgcgtcgcc cggcccgcgc ccgctaggcc ggcgaaagct cgaagacgca ggccttgcct
      301 tcccagagcg tctccgctac gcgaagctcc ctcgggtcgg ggagggccgc cgcgaggccc
      361 tggaaggggt tcacgcagga gatcgcgcag ggcgaggggg cgcctagccg cttcgcgagg
      421 gcgcaggcca ggcagttcga agcccgcgcg cggacgagcc cgccctcgcg ctcgaccttc
      481 cagtccgcgc agccgaagat ctccgcgaag gtcgcgaaca gcccctcggg cgtcccgatc
      541 ccgagccgcg cgagctgggc gggcgccgcg gcggcctgct cggccttctt gtcctcggtc
      601 acgcgctcga ggaccccctg tttcgcgtac tgtcgcgccg cgtcgacgac caggccggcg
      661 tagaaaagct gcagcgtctt gagcctcgtg gtctcgtcca tgtcccttcc tcctcgccgg
      721 ggcttgccgc cccgacgctt cgactatggc gcgtccggcc ccccgccggt atgatcagga
      781 atgatcattc gcgcgggaag gcgcgaccgg aggcccggct tggacgtgga aggctggaga
      841 cgcgagtacg aacgggtcct ctcccgggcc cgcctccgcg gggaggactg cgacgaggcc
      901 gtcctcgcgc gccacctccc cttcctgggg cggctcgacg cggtggaggg aagctcgatc
      961 gccctcttcg acttcaggac ccgctcctac cgcttcctca ccgcgagctt caggttcctg
     1021 ggcggctatc ccgtcctgga ggctcaggcg gaaggtccgg actacttctt ccgcgtcatg
     1081 cacgggcccg acctgccctt cgtcctcgag acgacggtca ggaccttcga gttcctggag
     1141 tccatccccc ccgcggagcg gaaggactgg aagctctcct tcgatttccg gatccgccac
     1201 gcgtcagggc gccttgtccg gcttctccag caggtcctcg tcctcgagct cgacgcacgg
     1261 ggcaacatct ggctcgtcct catcgcgaac gacctcgcgc ccaagggccc gggcgacgag
     1321 tccccctccc gcaggctgga gaacctaaag acgggcgagc gccgcctctt ccgcggcggg
     1381 gaggaggagg gcaagggcct gtcccggagg gagctcgaga tcctgggcct cgtggcggag
     1441 ggactgccga gccgcgagat cgcggagcgg ctgttcatca gcgtcgcgac ggtgaacaac
     1501 caccgcaggc ggatcctgga gaagctcgac acgcgctcga gcgccgaggc cgtgcgctgg
     1561 gcggccgccc gcggcctcgt gtaggagcgg gggcctgtcc tgaggccgga gcccctacga
     1621 acttcctcgt aatccccgag ccgggggcag gaggcggccc ttgccgggat agccgcgcgg
     1681 cgcgcccgag gcccatactg ggcggcgcca agaccgcgcg gcgatccgcg tcgagccgcg
     1741 agcggcaagg agggagcgat gatcatcgac ggccacggcc atgcctgcgg gccctatctt
     1801 tccagcgact ccatcgaccg ccacctggac gaggcagggg cggacctcgt cgtcctcacg
     1861 gcgggagagg cgggatcctc gaagacctac gccatcaagg acgacaccga gcggaggccc
     1921 gacgacgact acatggggcg gaacaaccgc ctcatcaagg tcttcgtcgc cttgagccgg
     1981 gccgcgcgcc gcatccgcgc cggcaacgag tccgtccacg ggctcgcgaa ggccaacccg
     2041 cgggtcctcc agtactactg ggcgagcggc gagagcgtcg aggagctcga gggcctctac
     2101 gggcgcatgc gcttccgggg actcaagctc cagcaggtct gggcccgcga ggacctgggc
     2161 gggaggcgct tcgacgagct cgaggagtgg ctcgccgcga agggcctgcc cctcttcatc
     2221 cacctcgctg accggaagga cgcgctcgcc ctggccgccc gcgccaggcg cggcctcagg
     2281 acgagcgtcg tcgtagccca cctctacggc ttcgaggccc tggaggagga gggggtcctc
     2341 ggcgcggagc ggctcctctt cgacgtctcg aacgcctact tcgtctcgcg gccgaggctc
     2401 gagcgcgccg tcgcgcgcgt cggcgcaggc cggctctgcc tgggctccga caccccctac
     2461 ggcgaggacg ccctgcgccg gaccatcgcg cggatcgact cgctcgccct gccggacggc
     2521 gagaaggcga agatcctcgg gcaaaacctc gcggagctcc tgcgcgtcga cggcgcggcc
     2581 gcgcggcgcc gcgcgtcggc gaggccgccc gccggcgcgc cggcggagac ggggagaggc
     2641 gaggatgcct agaggcgcgg aggcctcacg gaggctcgag gcggcggagc ggctcgtcgc
     2701 gcgggcggag gcgcccattc tctccgccgc cgtcgtcggc tcggtctcca cgggcttcgc
     2761 ggaccgggac tcggacatcg agctcgagct cttcgtcgag gagcttccga ccgaggccga
     2821 acgggaggac ctggccgcgc gcctcggcgg gctaagggtc gtccactatc ccgccccgct
     2881 cagggacggc tcggtctgga ccgtctgcga ctcgcctgag ggctggctgg agctcggctg
     2941 gcaggtcgag gagaaggcga tcgcggcgct cggggagctc cgccgcggcg cggtcctcga
     3001 ccacgagctc ctcgtcctcg cctcggccta cggccaggcc gcctggctca ggcgctcgga
     3061 ggcgatgggc gcggccatcg tcgggctcgg cgcgccctcc gaggagctgc gcgccgcgat
     3121 cgccgcggag gtcctgcggc agtgggacaa cgagctcgcc tggaaggtcc gggccacgct
     3181 ggtgcgccgc ggggacaggc tgcccttggc ccagcgccac gtcgccgacg cgcagcggat
     3241 gctgcggatc ctcttcgccc tgaacgggcg ctgggaaggc gactacaagt ggatcaagga
     3301 gcaggcagag ggactcccgc ggaagccttc ggacttcgcc gcgcgcctgg acctggtcct
     3361 cgcggcgccc gacggcgttt ccgccaacgc ggccctcgcg ggcctcctcg acgagctcct
//
LOCUS       DAOOSJ010000264         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18018_length_3410_cov_6.997317, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000264 DAOOSJ010000000
VERSION     DAOOSJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18018_length_3410_cov_6.997317"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..926
                     /locus_tag="PLG14_10995"
     CDS             141..926
                     /locus_tag="PLG14_10995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49702.1"
                     /translation="MPLVELPAGEGARVAPLFASFRLHRAHWGSVLAGANPGRVFADD
                     ARSPRLALVWPGDGFQYLAAEGEPGPALAELSSLLAREAKGACLELVVDDPRLEARLP
                     AIFGGLSYFSVPRLLFRLPPGWKASGPGGGDEAASGAAARGADAALSGASGPGAPARL
                     FRERGPSSIAVVLELGGRMAGRCRAWTWEGEAEADIFVEEESRGHGLGALLGREFLRY
                     CRDEGLEPVWSCWEDREESRRLALALGFELDRAFLVHIHDEVP"
     regulatory      1046..1197
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1437..3341
                     /locus_tag="PLG14_11000"
     CDS             1437..3341
                     /locus_tag="PLG14_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX49703.1"
                     /translation="MRKTLCAAALVLAVLGLGAEETKESLSGIVEVRAPAWSPLAAAP
                     GAPVTVVEAEEIEASGASTLAELLSGRSGVFVQRSGGGAGVASASVRGSTANQVLVVV
                     DGLRLNDSRQGGADLSQLPTASIERVEILSSGASAIYGPDALGGAIVVTTKRARESRL
                     SAELSNVSYPGALSEGGASALADTQRLAIDAGLVLGSADLALAATAERGLAAYPYGGA
                     ELRKNSAFSGGSARLSLSTPLAGGKLQAAFSGALRDADVAGSLAFPAEAEQGDKSLRG
                     SLSFSHDGLAGGLLSFVAQASGSWKRLEYYDPDPWYGADDRHDMATGGLDLRASWLAS
                     GNLEVGAGLTGLYEAADSTKFAASPGGQPSRFSLGGYLAPKYLLGERLVLAPVARFDW
                     AEDYPAGLSLMASLRWKASESLELRASGGTSYRAPTFNELYWPFEDFGGGYSYEGNPD
                     LRPETAMAGDLGAALSFGVLELSASGFARYVDDLIVSSAGAASTPVNLNKAFVPGASA
                     KATLAMAGFEVAADYEFLYPLDLSGGVALADAPRIESLSAHRADLSASYRQAAFEGRF
                     ALRYASEKTVSGTAIPGAAILDLGAGYKIGEGLKLGLKAENLLDTSYEVVSGYPMPGR
                     SLTTSLKVEL"
BASE COUNT          449 a         1265 c         1190 g          506 t
ORIGIN      
        1 ggctacgaga agttcctctt cgacgtggtc ttcggcgagg agacggaggt cgaggtcctc
       61 gagtccgtcc tcgcgggcga cgcgcgctgc cgcttcaaga tcaggattcc cgacgcggtc
      121 ctgcgctcca agggggcctg atgccgctcg tcgagctgcc cgcgggagag ggagcgcgcg
      181 tcgcgcccct cttcgcctcc ttccgcctcc accgggcgca ttggggctcc gtcctcgccg
      241 gagccaatcc cggcagggtc ttcgccgacg acgcccgttc gcctcgcctc gccctggtct
      301 ggcccgggga cggcttccag tacctcgccg cggaaggcga gcccggaccg gccctcgcgg
      361 agctgtcgtc cctcctcgcc cgggaggcga agggcgcctg cctcgagctc gtcgtggacg
      421 atccccgcct cgaggcgcgc cttcccgcga tcttcggcgg cctctcctat ttctcggtcc
      481 cgcgcctcct tttccgcctg cccccgggct ggaaggccag tggcccgggg ggcggcgacg
      541 aggcggcttc cggcgccgcg gcgcgcggcg ccgacgccgc gctttccggc gcctcggggc
      601 cgggcgctcc ggcgaggctc ttccgcgagc gcgggccctc ctcgatcgcc gtcgtcctgg
      661 agctcggcgg acggatggcc ggccggtgcc gggcctggac ctgggagggc gaggccgagg
      721 cggacatctt cgtcgaggag gagtcgaggg gccatgggct cggtgccctc cttggccgcg
      781 agttcctccg ctactgccgc gacgagggcc tcgagcccgt ctggagctgc tgggaggatc
      841 gggaggagtc caggcggctc gccctcgccc tcggcttcga gctggaccgc gccttcctgg
      901 tccacatcca cgacgaggtt ccctagcggc caaggccaat tggcggggcg ccgcagggtg
      961 gcgacgccgc ggtctccgcg cggccgcctt gacggcgccg ccggggccgc gctagagtgg
     1021 gcccgacacg agccgcgggg atcgtggaat gcggtgagag accgcagcgg acccgccact
     1081 gtaaccgggg atgagttcga ccttgcgccg ccgcgaaagc gacgggcgcg gccattgcgg
     1141 ccctttcagg ctgcgagaag gcgtcgacga cgtcagacga tccggaagcc aggacaccga
     1201 cccgcggctt gcctcgacgc tcccacctgg ggagcgcggg gcgaaacttc gaggacctga
     1261 ggatcgccgc gcgcgccgtt ccacaagccc gggccgccca tcggcccgcg ccgtccgccc
     1321 gggaaggcct tcccgaggcg ggaaagcgta cagcagcaga agtccatcgt cgcatgacgc
     1381 gaccctccag gtcctccgtc ggcccgcctg aacggcggag ccggaaggag catcggatgc
     1441 gcaagactct gtgcgcggcc gccctcgtcc tcgcggtact gggactgggc gcggaagaga
     1501 ccaaggaatc cctctcggga atcgtcgagg tgcgggcgcc ggcctggagc ccgctcgccg
     1561 ccgcgccggg ggcgccggtc acggtcgtgg aggccgagga gatcgaggcg agcggcgcat
     1621 ccaccctcgc ggagctgctc tcgggcaggt cgggcgtctt cgtccagcga agcggcggcg
     1681 gagcgggcgt cgcatccgcc tcggtccgcg gttccaccgc gaaccaggtc ctcgtcgtgg
     1741 tcgacggcct ccgcctcaac gattcgcggc agggcggagc cgacttgagc cagctcccga
     1801 ccgcctccat cgagcgcgtg gagatactct ccagcggcgc gagcgcgatc tacggccccg
     1861 acgccctggg cggcgccatc gtcgtcacga cgaagcgcgc gagggagagc cgtctctccg
     1921 ccgaactctc caacgtctcc tatcccggcg ccctgtcgga gggtggagcc tcggccctcg
     1981 ccgacaccca gcgactcgcc atcgacgcgg gactcgtcct cggctccgcc gacctcgcgt
     2041 tggccgccac cgccgagcgg ggcctcgcgg cctatcccta cggcggcgcc gagctcagga
     2101 agaattccgc cttctcgggc gggagcgcgc ggctatcact ctccacaccc ctggccggag
     2161 gcaagctcca ggccgctttc tccggtgcgt tgcgcgacgc cgacgtcgcg ggctccctgg
     2221 ccttccccgc cgaggcggag cagggcgaca agtcccttcg cggctccctg tccttctcgc
     2281 acgacggact ggcgggcgga ctcctctcct tcgtggccca ggcgagcggc tcgtggaagc
     2341 ggctcgagta ctacgatccc gatccctggt acggggcgga cgaccggcac gacatggcga
     2401 cgggaggcct cgacctccgc gcctcctggt tagcgtccgg gaatctcgag gtgggcgccg
     2461 ggcttacggg gctctacgag gccgcggaca gcacgaagtt cgccgcgagc ccgggcggac
     2521 agccctcccg tttctccctg ggcggctatc tcgcccccaa gtacctactc ggcgagcgcc
     2581 tcgtcctcgc tcccgtcgcg cgcttcgact gggcagaaga ctacccggcg ggcttgagtc
     2641 tcatggcgag cctcaggtgg aaggcctcgg agtcgctcga gcttcgggcc tccggcggaa
     2701 cctcctaccg ggcgccgacc ttcaacgagc tctactggcc tttcgaagac ttcgggggcg
     2761 gctacagcta tgaaggcaat cccgacctca ggccggagac cgcgatggcg ggcgacctcg
     2821 gtgcggccct ctccttcggc gtcctggagc tctccgcctc cggcttcgcg cgctacgtcg
     2881 acgatctcat cgtatccagc gccggcgcgg ccagcacgcc cgtcaacctc aacaaggcct
     2941 tcgtccccgg cgcctcggcc aaggccaccc tcgcgatggc cggattcgag gtcgccgccg
     3001 actacgaatt cctctatccc ctcgacctct cgggcggggt cgcgctggct gatgcgccgc
     3061 gcatcgagag cctctctgcc caccgggctg atctatcagc ttcatacagg caagccgcct
     3121 tcgagggtcg gttcgccctc cgctacgcga gcgagaagac tgtctcgggg acggcgatcc
     3181 ccggcgccgc catcctggac ctcggcgcgg gctacaagat cggcgagggc ctgaagctcg
     3241 gcctcaaggc ggagaatctc ctcgacacga gctacgaggt ggtgtcgggc tacccgatgc
     3301 ccggcaggag cctcacgacg agcctcaagg tcgagctgta ggcgccgcgg ggcaggcctc
     3361 ggcggcccgc ccgcgcggcg gccgtacggc cgtttgcgag tccgcgaagg
//
LOCUS       DAOOSJ010000265         3366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18356_length_3366_cov_6.415886, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000265 DAOOSJ010000000
VERSION     DAOOSJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18356_length_3366_cov_6.415886"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /gene="zapB"
                     /locus_tag="PLG14_11005"
     CDS             complement(<1..247)
                     /gene="zapB"
                     /locus_tag="PLG14_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOX49704.1"
                     /translation="MLSVEQVHALEGRVEKALAYISSLRTENADLRRRLEKAEGDLGL
                     AAARAEELEAAAESFRRDQSRIEEGIVHALEKLDAFED"
     gene            complement(254..613)
                     /gene="rplT"
                     /locus_tag="PLG14_11010"
     CDS             complement(254..613)
                     /gene="rplT"
                     /locus_tag="PLG14_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOX49705.1"
                     /translation="MPRAVDGTKRKDHRKKILKITKGFWGRRHSNYKVAKDAGAKALS
                     YAYRDRRDRKGTFRQLWIARINAAVRAEDLSYSRFIQGLNKAQIVVDRKMLADMAVRD
                     PAGFKAVVEKAKAALKA"
     gene            complement(727..927)
                     /gene="rpmI"
                     /locus_tag="PLG14_11015"
     CDS             complement(727..927)
                     /gene="rpmI"
                     /locus_tag="PLG14_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOX49706.1"
                     /translation="MPKMKTKKAAAKRFTLTASGKVKYKKMNLRHILTKKTTKRKRGL
                     RKSGYVDPVATYAIKKTLLPYG"
     gene            complement(1079..1588)
                     /gene="infC"
                     /locus_tag="PLG14_11020"
                     /pseudo
     CDS             complement(1079..1588)
                     /gene="infC"
                     /locus_tag="PLG14_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            1849..2295
                     /locus_tag="PLG14_11025"
     CDS             1849..2295
                     /locus_tag="PLG14_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49707.1"
                     /translation="MTTTTLGRALAQARARNDALVAAYKSVARRALSRAISSFAASAA
                     DQRRELGLSLAEMLAALPESLSGLELPAEAAPALEAVDAETAEARALLASFRDEEAQD
                     RKLFADLAAAAAAVDQDAAIRLGAFAEQARKRSGLVGDHLDLLSLG"
     gene            2373..3023
                     /locus_tag="PLG14_11030"
     CDS             2373..3023
                     /locus_tag="PLG14_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49708.1"
                     /translation="MYAFLLFGLPLAYVLLILRVYPREDAQATRRATVRGLLAAIPVW
                     LLARLLGNLVPAAWGSPLLAFHEWADRFLPYAVLPALAYAVFYHYGERLPSGQAARRL
                     TAFYAGALAPLGLGEMTRIWGNPSAYSVLVLPLLFTALALAMPALVLAFFEEYGARRA
                     LPVLAAVLATLAASLVRWLFLARFWPLALLLVAGLAAASWLYAAPGLLTRPPRPAA"
     gene            complement(3164..>3366)
                     /locus_tag="PLG14_11035"
     CDS             complement(3164..>3366)
                     /locus_tag="PLG14_11035"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOX49709.1"
                     /translation="KAAKSAVCKINIDSDGRLAMTAMIRKVFTEKPDEFDPRKYLGPA
                     RDELKKLYAHKNLEVLGSAGKA"
BASE COUNT          417 a         1198 c         1105 g          646 t
ORIGIN      
        1 ggtcctcgaa ggcgtcgagc ttctcgaggg cgtggacgat gccctcctcg atgcgggact
       61 ggtcgcggcg gaaggactcc gccgcggcct cgagctcctc cgcgcgggcg gcggcgaggc
      121 cgaggtcgcc ctcggccttc tccagcctgc gcctgaggtc ggcgttttcc gtcctgaggg
      181 aggagatgta ggctagggcc ttctctaccc ggccctccag ggcgtgtacc tgctcgacgc
      241 tcagcatttg cgcttaggcc ttgagggcgg ccttggcctt ctcgacgacg gccttgaagc
      301 cggcgggatc gcggacggcc atgtcggcga gcatcttgcg gtcgacgacg atctgggcct
      361 tgttgagtcc ctggatgaag cggctgtagg aaaggtcctc ggcgcggacg gccgcgttga
      421 tgcgggcgat ccagagctgg cggaaggtgc cctttctgtc gcggcggtcg cggtaggcgt
      481 aggagagggc cttcgcgccc gcgtccttgg ccaccttgta gttggagtgg cggcggcccc
      541 agaagccctt cgtgatcttg aggatcttct tgcggtggtc cttgcgcttc gttccgtcta
      601 ctgctctagg catggatatt ctcctcgttc gtggtggcgg ccgccctcag gggcgccgcg
      661 gttcgggcct cgccggacag gattcccgga cgcggccgat ggtctaaaag tgtcaggaag
      721 cccggctcag ccgtagggga ggagggtctt cttgatcgcg taggtggcga ccgggtcgac
      781 gtagccggac ttgcggagcc cgcgcttgcg cttggtcgtc ttcttggtga ggatatggcg
      841 aaggttcatc ttcttgtact tgaccttgcc ggaggccgtc agcgtgaaac gcttggcggc
      901 ggccttcttc gtcttcatct taggcatggt gatccatcct tccttgatat ttccgctttt
      961 acgcttccgg agcctgggcg ggggccgagg cggcggaagg ctgggcgggg ggcgtttcgg
     1021 ggcccgaggc cgcggcagag gccgcggcga gctcttcggc gctcttcttg ataatggaag
     1081 ccttcgggct cagcaccatg gacatgaagc gcccctccat ggaaggggtc ttctccatga
     1141 tgtagtagcc gtcgatgcgg gagaggacct gcttgaggac gtcctcgccc ctttcggtgt
     1201 gggcgagctc gcgtccgcgg aagcggatcg tcaccttgac cttgttcccc tcctccagga
     1261 agccgcgcac gtgcttggcc ttgaagtcga ggtcgtggcc ggcgatcttg ggctgcatcc
     1321 ggatctcctt gagctctccg cccttggcct tcttccggga gtccttgatc ttcttttcct
     1381 gctcgaactt gaacttgccg tagtccagca gcttgcacac gggcggaacc gactggggag
     1441 cgacctcgac tagatcgacg ccgagatcct tggcgagctt gatcgcctcg agcgtcggga
     1501 cgatgccccg ctgctcgccc ttgtcgtcga tgagacgcac ctcgcggacg cggatctgtt
     1561 cattgatccg caagtctttg tctgccaact gacctccttc ggcgaattcc cccggcttcg
     1621 ggcccaaggg cccgccaggg gaatagtccg ctcgagcgga actgtggcgc gtattgtagc
     1681 acgttccgcc gagcctcgtc cagggctttg ggccgccggg aaagagccat aatgccccgc
     1741 ccggccgtct ttcgggctcg cggaaggact cttggggcgg aaaaccggga aagaggcatt
     1801 ctatagtaca atttgacagg gatttcaagg acgggtacac tcaaagccat gacgaccacg
     1861 acgctcggaa gagccctcgc ccaggcccgc gcccggaacg acgcgctcgt cgcggcctac
     1921 aagtcggtcg cgcgccgggc tctttcccgc gcgatctcga gcttcgccgc ctccgccgcc
     1981 gaccagcgcc gcgagctcgg cctctccctc gcggagatgc tcgcggccct gcccgagtcc
     2041 ctctcgggcc tggaactccc cgcggaggcc gccccggcgc tcgaggccgt cgacgccgag
     2101 accgcggagg cccgagccct cctcgccagc ttccgggacg aggaggctca ggaccgcaag
     2161 ctcttcgccg acctggccgc ggccgccgcg gccgtcgacc aggacgccgc gatccgcctc
     2221 ggagccttcg cggagcaggc ccgcaagcgc tcgggcctcg taggcgacca cctcgacctc
     2281 ctctccctgg gctgatccgg cggcgggcgg gccgacgtcg gtcccgggcg gcggcttgaa
     2341 ggctccagtc gcgctgtgct agccttgagt ccatgtacgc cttcctcctc ttcggcctgc
     2401 ccctggccta tgtcctcctc atcctccgcg tctatcccag ggaggacgcc caggccacgc
     2461 ggcgagcgac cgtccgcggc ctcctggccg cgattcccgt ctggctcctc gcccgcctcc
     2521 tcgggaacct cgtgccggcg gcctggggct cgcccctcct cgccttccac gagtgggccg
     2581 accgcttcct cccctacgcc gtcctgcccg ccctggccta cgcggtcttc taccattacg
     2641 gcgagcgcct cccctcgggc caggccgcgc gccggctcac cgccttctac gcgggagccc
     2701 tggcccccct cgggctcggc gagatgacgc ggatctgggg caacccgagc gcctactccg
     2761 tcctcgtcct ccccctcctg ttcacggccc tggccctcgc catgcccgcc ctggtcctcg
     2821 ccttcttcga ggagtacggc gcgaggcgcg ccctccccgt cctggccgcg gtcctcgcca
     2881 ccctcgcggc ctcgctggtc cggtggctct tcctggcccg cttctggccc ctcgccctcc
     2941 tccttgtcgc cggcctcgcg gccgcctcct ggctctacgc cgcccccggc ctcctcaccc
     3001 gcccgccccg ccccgccgcc tagccaagag cggcctgctt gcgttgcggg cgcggcccgc
     3061 gcggcggcca gcctgactga atccgcctca agcgctgaag cgcaaaaaag cgccccggtc
     3121 acccggggcg cttctcatgg caagaggaga gggtccggcg cccttaggcc ttgccggcgg
     3181 agccgagcac ctcgaggttc ttgtgcgcgt agagcttctt gagctcgtcg cgcgccgggc
     3241 cgaggtactt gcggggatcg aactcgtccg gcttctcggt gaagaccttg cggatcatcg
     3301 cggtcatcgc gaggcggccg tcggagtcga tgttgatctt gcagacggcg ctcttcgcgg
     3361 ccttgc
//
LOCUS       DAOOSJ010000266         3336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18621_length_3336_cov_6.396525, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000266 DAOOSJ010000000
VERSION     DAOOSJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_3336_cov_6.396525"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLG14_11040"
     CDS             <1..454
                     /locus_tag="PLG14_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOX49710.1"
                     /translation="LLLSRLGAPSGLPAEALALLCGGAAVAGHVWTVFAGFRGGKGVA
                     TAAGALLAAAPLPAAFAALAFALVLLSTGVVSLSSLAAALAFPATLLAGLALGRPVSP
                     LLLGFSLVLAPLIFYTHRSNLARLRRGEEKRFERLRVLGRLLDRFRRR"
     gene            complement(813..1652)
                     /locus_tag="PLG14_11045"
     CDS             complement(813..1652)
                     /locus_tag="PLG14_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOX49711.1"
                     /translation="MTTILITLGFSALLAFVLGALLGFFREKFKVERDPLVDEVRGAL
                     PGANCGGCGYPGCDGYAEAVAARRAAPNLCSAGGSKTAEAVAKIVGVEAAAEDLVSVL
                     LCGGVKEKALDKGSYVGLPTCRGAKLSTGSVKACAWGCQGFGDCVEVCKFDALSMGED
                     GLPHVDYDKCTGCGKCAEECPQRLFAMVPKSRKGSVVLCSNRNVLKANVMKACKAGCI
                     KCEACVKICPEACIAMVNGIPVTDYAKCTSCGKCVEKCPTKCYRLLEVDVMGAKKKVP
                     VLA"
     gene            complement(1663..2232)
                     /locus_tag="PLG14_11050"
     CDS             complement(1663..2232)
                     /locus_tag="PLG14_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOX49712.1"
                     /translation="MTKIFIIALLINNVVLMRFLALCSYVGMTSDVGQSVGMGFAVTF
                     VTVLATAATWPLYNFVLLPLGLGFLQILVFILVIASLVQLVEFYLKKNVPGLYAAMGI
                     YLPLITTNCAILAVTFENISNGYNFIESIVYAVGVSLGYLLAMVLLAGVRIRIKNSPV
                     PKFLKGTPILFAATSLIAIAFMGFSGLVK"
     gene            complement(2229..2870)
                     /gene="rsxE"
                     /locus_tag="PLG14_11055"
     CDS             complement(2229..2870)
                     /gene="rsxE"
                     /locus_tag="PLG14_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOX49713.1"
                     /translation="MKRLWKIFKAGIIYENPLLMLMIGLCSALAVTTNVSNGVGMGLA
                     MTFVMLFSEVIISLFRKVIPNSARMPIFIIVIAAFTTIIDLVMKAYFPELSKSMGVFI
                     PLIVVNCIIMGRVEAFASKEKVLDCVADSLGMGLGYTWVLVGIAAFRELLGNGTLAGL
                     QVMPASFEPILFFILPPGGFLVFALFISLNIWLKGRMQKASAKAEAKAEEVAA"
     gene            complement(2872..>3336)
                     /locus_tag="PLG14_11060"
     CDS             complement(2872..>3336)
                     /locus_tag="PLG14_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOX49714.1"
                     /translation="EKFVDATAELKIADPAVALEAAYLAERGGAPLGVAVKAKGQSYG
                     GPTTVLVGVALDRRIAGVRILDGKDTPGLGANAMNPTFFVDKSKKTTFPGQFSGKALS
                     DPFEVKNDVVAITASTITSKAITKMIKASADAASARLEAFAAAPAEAAPGGK"
BASE COUNT          529 a         1138 c         1156 g          513 t
ORIGIN      
        1 cctcctcctc tcgcgcctgg gcgcgccctc gggcctcccc gcggaggccc tggccttgct
       61 ctgcggcggc gcggccgtcg ccggccacgt ctggaccgtc ttcgcgggct tccgcggcgg
      121 caagggcgta gccaccgcgg ccggcgccct cctcgcagcg gcgcccttgc ccgcggcctt
      181 cgcggccctc gccttcgccc tcgtcctcct ctccacgggc gtcgtctccc tctcctccct
      241 cgcggccgcc ctcgccttcc ccgccaccct cctcgcgggc ctggccctcg gccgccccgt
      301 gtcgcccctc ctcctcggct tctccctcgt cctcgcgccc ctcatcttct acacccaccg
      361 ctccaacctg gcccgcctcc gccgcggcga ggagaagcgc ttcgagcgcc tccgcgtcct
      421 cggccgcctc ctcgaccgat tccgccgccg ctagagcgga tcgcggcctc gccgccgcgg
      481 ctaggtcggg ctcgaggcgc cgcttccgcc cgccccgccc cgcccgcgcc gccgggacaa
      541 gacgcaggtc ccctcatcga gcccctctcc gcgaggcgcc gcccatccgg caggccccta
      601 gttgttgaac cccgcccgag cctcgcgagg ctcaggcagg acagcgggtg gcgggggacg
      661 cgcggggacg gagcggagcc cgcggagctc ctgtttccgc gcgccccccg ccaggccccg
      721 ccctgcaaag cttccaaaga cccgagcaag cgcaaaataa aaagggggct gcccgacagg
      781 cagccccctt gcgttcacgg agagaaacgc ggctaggcga ggacgggcac cttcttcttc
      841 gcgcccatga cgtcgacctc gaggagcctg tagcacttgg tcgggcactt ctcgacgcac
      901 ttgccgcagc tcgtgcactt cgcgtagtcg gtgacgggga tgccgttgac catcgcgatg
      961 caggcctccg ggcagatctt cacgcaggcc tcgcacttga tgcagccggc cttgcaggcc
     1021 ttcatcacgt tggccttgag gacgttgcgg ttggagcaga ggaccaccga gcccttgcgg
     1081 gacttgggca ccatcgcgaa gaggcgctgg gggcactcct ccgcgcactt gccgcagccc
     1141 gtgcacttgt cgtagtcgac gtggggcagg ccgtcctcgc ccatggagag ggcgtcgaac
     1201 ttgcagacct cgacgcagtc gccgaagccc tggcagcccc aggcgcaggc cttcacggag
     1261 cccgtggaga gcttggcgcc gcggcaggtg ggaaggccga cgtagctgcc cttgtccagg
     1321 gccttctcct tgacgccgcc gcagagcagc acggagacca ggtcctcggc cgccgcctcg
     1381 acgccgacga tcttcgcgac ggcctcggcc gtcttggagc cgccggcgga gcagaggttc
     1441 ggggcggcgc gccgggcggc caccgcctcg gcgtagccgt cgcagccggg atagccgcag
     1501 ccgccgcagt tggcgccggg aagggcgccg cgcacctcgt ccacgagggg gtcgcgctcg
     1561 accttgaact tctcgcggaa gaagcccagg agggcgccca ggacgaaggc gaggagggcg
     1621 gagaagccga gcgtgatcag tatggtcgtc atgccgtcct cctcacttca ccaggcccga
     1681 gaagcccatg aaggcgatgg cgatgaggct cgtcgccgcg aagaggatgg gcgtcccctt
     1741 caggaacttg ggcacggggc tgttcttgat gcggatgcgg acgccggcca ggaggaccat
     1801 cgcgaggagg tagcccagcg agacgccgac cgcgtagacg atcgactcga tgaagttgta
     1861 gccgttggag atgttctcga aggtcacggc caggatggcg cagttcgtcg tgatgagggg
     1921 caggtagatg cccatcgcgg cgtagaggcc cggcacattc ttcttcaggt agaactccac
     1981 gagctggacc agcgaggcaa tcaccaggat gaagaccagg atctggagga agcccaggcc
     2041 gagcggcagg aggacgaagt tgtagagcgg ccaggtcgcc gcggtcgcca ggaccgtgac
     2101 gaaggtgacc gcgaagccca tgccgaccga ctggccgacg tcgctcgtca tgccgacgta
     2161 ggagcagagc gccaggaagc gcatcaggac gacgttgttg atgaggagcg cgataatgaa
     2221 gatcttggtc atgcggccac ctcctcggcc ttcgcctcgg ccttggccga cgccttctgc
     2281 atgcggccct tgagccagat gttgagggag atgaagaggg cgaagacgag gaagccgccc
     2341 ggcgggagga tgaagaagag gatgggctcg aaggaggcgg gcatcacctg gaggccggcg
     2401 agtgtgccgt tccccaggag ctcgcggaag gccgcgatgc ccacgaggac ccaggtgtac
     2461 ccgaggccca tgcccagcga gtccgccacg cagtccagga ccttctcctt ggaggcgaag
     2521 gcctccacgc ggcccatgat gatgcagttg acgacgatga gggggatgaa gacgcccatc
     2581 gacttggaga gctcggggaa gtaggccttc atcaccaggt cgatgatcgt cgtgaaggcg
     2641 gcgatgacga tgatgaagat cggcatgcgc gccgagttcg ggatcacctt cctgaacagg
     2701 gagatgatca cctcggagaa gagcatgacg aaggtcatcg ccaggcccat gccgacgccg
     2761 ttggagacgt tggtcgtgac ggccagggcc gagcagaggc cgatcatgag catgaggagg
     2821 ggattctcgt agatgatccc cgccttgaat atcttccaga gtcgcttcat gtcacttccc
     2881 tcccggggcg gcttccgcgg gggccgcggc gaaggcctcg agcctggccg aggcggcgtc
     2941 ggcggaggcc ttgatcatct tggtgatcgc cttcgaggtg atggtcgagg cggtgatcgc
     3001 gacgacgtcg ttcttgacct cgaagggatc ggagagggcc ttgcccgaga actggccggg
     3061 gaaggtggtc ttcttcgact tgtcgacgaa gaaggtcggg ttcatggcat tggcgcccag
     3121 gcccggggtg tccttgccgt ccaggatcct gacgccggcg atccggcggt cgagcgccac
     3181 gccgaccagg acggtggtgg ggccgccgta gctctggccc ttcgccttca cggcgacgcc
     3241 caggggcgcg ccgccgcgct cggccaggta ggcggcctcc agggccacag cggggtcggc
     3301 gatcttgagc tcggcggtgg cgtcgacgaa cttctc
//
LOCUS       DAOOSJ010000267         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18739_length_3320_cov_6.263093, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000267 DAOOSJ010000000
VERSION     DAOOSJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18739_length_3320_cov_6.263093"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2976)
                     /locus_tag="PLG14_11065"
     CDS             complement(<1..2976)
                     /locus_tag="PLG14_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOX49715.1"
                     /translation="MYFNELVAAYDQARRERNVFHDLMRFRVREVLLVGSLYDSFVVE
                     SDGVLTEQIYGEYFKLNLSTVPRITCAYTDEAALELFQEGRFDLVILMAGLDFERPLL
                     LAKAMKAIWPGIPVLLLVTNNTGLESLGPERAGLEAIDRVFVWNGYSKLFVGMMKYVE
                     DMRNAEDDTRSGLVRVILLIEDSVRYYSRYLPVLYKVILRQTQGLIEEEKAVETYKLL
                     RIRARPKVLLASSYEEAQALFARYEPYLLAVISDLRFPRNGALDPEAGFEFLGMAKSR
                     IADLPVMIQSSEIGVRERAHALGAAFADKESESLEHELASFLQENLGFGPFRFRAPAA
                     GGGEGEVLAEARNMREFMALLDSVPPESLLMHAGRNHFSAWLLARGEIQFARLLKNYR
                     IDDFSSVQEIRGFILRVLEGARRDKSRGMVPNFDESMCRDENCMMRLGDGSVGGKGRG
                     LLFIKSLLDNLDLRNELPGLEVKIPLTAFIGIDEFERFLELNGLWSFAYYEASSEEVR
                     GRFLAAPLSPELTERLRRFVAVTDKPLAVRSSGLFEDMLMVPFSGIYDTYLIPNAHPV
                     PERRLAQLCDAVRLIYASLFSRKARAYFDAARYKIEEERMAVVVEELVGARRGRWFYP
                     HVSGTAQSFNYYPVSYVRPEDGLCVSALGLGSYVVDGGAAHQFCPRWPKLDVVAPERA
                     RESSQRVFRALDMERLEPELARGEDAALVDLPLSEAEADPRFALLASTYDLANDRLVP
                     GIGAKGPRFVDLSNILKHEALPFAKAVDLVLDLGARSMATPVEIEYALNLDEASGKPA
                     LYLLQLKPLIRTEDKVEVDLADADPGECLIACERSMGNGRDAAIRDVVWVDPKRFDRA
                     ATQEIALEVEELDRALGEAGRRYILIGPGRWGTRDPWLGVPVVYSQISRARVIVEADL
                     PGFRVESSLGSHFFHNVTSMNIGYLTVPANGGLSFVDWDWLASFPPERRSAHCAWTSL
                     PEPLEILMDGRRSKAIVRKK"
     gene            complement(3067..>3320)
                     /locus_tag="PLG14_11070"
     CDS             complement(3067..>3320)
                     /locus_tag="PLG14_11070"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOX49716.1"
                     /translation="TRVIHYYLWFNPAVESQATDRAFRIGQTRDVFVHRLVTKGTLEE
                     RIDAALASKRELADLAVGAGETWLSELGNAELKELVALR"
BASE COUNT          521 a         1041 c         1269 g          489 t
ORIGIN      
        1 cttcttcctc acgatggcct tgctccgcct cccgtccatg aggatctcca ggggctcggg
       61 caggctggtc caggcgcagt gggcgctccg gcgctcgggc gggaaggagg cgagccagtc
      121 ccagtcgacg aaggagaggc cgccgttcgc gggcacggtg aggtagccga tgttcatgct
      181 cgtcacgttg tggaagaagt ggctccccag ggaggactcc acgcggaagc ccgggaggtc
      241 ggcctcgacg atgacgcggg cgcgcgagat ctgcgagtag acgacgggga cgccgagcca
      301 ggggtcgcgg gtgccccagc gcccggggcc gatgaggatg tagcgcctcc cggcctcgcc
      361 cagtgcgcgg tcgagctcct ccacctccag ggctatctcc tgggtcgccg cgcggtcgaa
      421 gcgcttaggg tcgacccaga cgacgtcgcg tatcgcggcg tcgcggccgt tccccatgct
      481 ccgctcgcag gcgatgaggc actcgccggg gtcggcgtcc gccaggtcca cctcgacctt
      541 gtcctccgtc ctgatgaggg gcttgagctg gaggaggtag agggcgggct tccccgaggc
      601 ctcgtcgagg ttgagcgcgt actcgatctc cacgggagtc gccatggagc gggccccgag
      661 gtccaggacg aggtcgaccg ccttggcgaa gggcagggcc tcgtgcttca ggatgttcga
      721 gaggtcgacg aagcgcgggc ccttcgcccc gatgcccggg acgagacggt cgttcgcgag
      781 gtcgtaggtg gaggcgagga gggcgaagcg ggggtcggcc tccgcctcgg agaggggaag
      841 gtcgaccagg gcggcgtcct cgccccgggc gagctcgggc tccaggcgct ccatgtcgag
      901 ggcgcggaag acccgctggg aggactcgcg cgcccgctcc ggcgcgacca cgtcgagctt
      961 gggccagcgg gggcagaact ggtgggccgc gccgccgtcg acgacgtagg agcccaggcc
     1021 cagggccgag acgcagaggc cgtcctcggg gcggacgtag gagaccgggt agtagttgaa
     1081 ggactgggcc gtgcccgaga cgtgggggta gaaccagcgc ccgcgccgcg cgcccacgag
     1141 ctcctccacc acgacggcca tgcgctcctc ctcgatcttg tagcgggccg cgtcgaagta
     1201 ggcgcgggcc ttccgcgaga agaggctcgc gtagatgagg cggaccgcgt cgcagagctg
     1261 ggcgaggcgc cgctccggca cgggatgggc gttcgggatc aggtaggtgt cgtagatgcc
     1321 ggagaagggc accatgagca tgtcctcgaa gaggcccgag gagcggacgg ccaggggctt
     1381 gtccgtgacg gccacgaagc ggcggaggcg ctccgtgagc tcgggggaga ggggggcggc
     1441 gaggaagcgc ccgcggacct cctcgctcga ggcctcgtag taggcgaagg accagaggcc
     1501 gttcagctcc aggaagcgct cgaactcgtc gatgccgatg aaggcggtga gggggatctt
     1561 cacctccagg cctggcagct cgttccggag gtccaggttg tcgaggaggc tcttgatgaa
     1621 gaggaggccc cgccccttcc ctcccaccga gccgtcgccc aggcgcatca tgcagttctc
     1681 gtcgcggcac atcgactcgt cgaaattcgg gaccatgccg cgggacttgt cgcgcctcgc
     1741 gccttccagg acgcggagga tgaagccgcg gatctcctgg acggaggaga agtcgtcgat
     1801 gcgatagttc ttgaggaggc gggcgaactg gatctcgccg cgggcgagga gccaggcgga
     1861 gaagtggttg cggcccgcgt gcatgaggag gctctcgggc ggcaccgagt cgaggagggc
     1921 catgaactcg cgcatgttgc gcgcctccgc gaggacctcg ccctcccctc cccccgccgc
     1981 cggggctcgg aagcggaagg ggccgaagcc caggttctcc tggaggaagg aggcgagctc
     2041 gtgctccagt gactcggact ccttgtcggc gaaggccgcg cccagggcgt gggcccgctc
     2101 gcggaccccg atctcgctcg actggatcat cacggggagg tcggcgatcc gcgacttcgc
     2161 catgccgagg aattcgaagc cggcctcggg gtccagggcc ccgttccgcg ggaaacggag
     2221 gtcggagatg acggcgagga ggtagggctc gtagcgggcg aagagggcct gggcctcctc
     2281 gtagctcgag gcgaggagga ccttgggcct ggccctgatg cggaggagct tgtaggtctc
     2341 caccgccttc tcctcctcga tgaggccctg ggtctgcctc aagatgacct tgtagaggac
     2401 ggggaggtag cgcgaatagt agcgcaccga gtcctcgatg aggaggatga cgcggaccag
     2461 gccgctcctc gtgtcgtcct ccgcgttccg catgtcctcc acgtacttca tcatgccgac
     2521 gaagagcttg gagtagccgt tccagacgaa gacgcggtcg atcgcctcca ggccggcgcg
     2581 ctccgggccc agggactcga ggcccgtgtt gttcgtgacg aggaggagga cggggatgcc
     2641 cggccagatg gccttcatcg ccttggccag gaggaggggc cgctcgaagt cgaggcccgc
     2701 catgaggatg acgaggtcga agcggccctc ctggaagagc tcgagggcgg cctcgtcggt
     2761 gtaggcgcag gtgatccgcg gcaccgtgga gaggttcagc ttgaagtact cgccgtagat
     2821 ctgctcggtg aggacgccgt ccgactcgac gacgaaggag tcgtagaggc tgccgacgag
     2881 gaggacctcc cggacgcgga agcgcatgag gtcgtggaag acgttccgct cccgcctggc
     2941 ctggtcgtag gccgctacga gctcgttgaa gtacatgggg cctcctcgcc gccggacgcc
     3001 gcgtcgccgc ggccccggcc aggctagagc atagcgcgaa ggggccttgc gaggaagggc
     3061 tccggctcag cgcagggcga cgagctcctt gagctcggcg ttcccgagct ccgagagcca
     3121 ggtctccccg gccccgacgg cgaggtcggc gagctcgcgc ttcgaggcga gggccgcgtc
     3181 gatccgctcc tccagcgtcc ccttggtcac gaggcgatgg acgaagacgt cgcgcgtctg
     3241 gccgatgcgg aaggcgcggt cggtggcctg gctctccacg gcggggttga accagaggta
     3301 gtagtggatg acgcgggtcg
//
LOCUS       DAOOSJ010000268         3320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18741_length_3320_cov_4.272894, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000268 DAOOSJ010000000
VERSION     DAOOSJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18741_length_3320_cov_4.272894"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1530
                     /locus_tag="PLG14_11075"
     CDS             190..1530
                     /locus_tag="PLG14_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX49717.1"
                     /translation="MKPPFSRRVPWPAEPNALSSLLAERRRAGLPILNFAESNPTRVG
                     LARDGGALLGALAVEASLRYAPEPRGLPGPREALAAFYAGRGTLPLARGPAASETAAW
                     GEAASGAAASRTANGAAASGTAGPPAGDAEAAGPLLGPDRFFLCASTSEAYGWLFKLL
                     CEPGEAVLVPKPGYPLFDYLAGLEGVEARPYRLEYFHPKGWRIDLESLEAALADGLAR
                     AVVLINPNNPTGSYVGAEERRAVVELAARHGAALVVDEVFFGFPLEAESASSFAGEEG
                     VLTFVLDGLSKLLGLPQMKLGWIAASGPAPELAEALGRLEIVADTYLSAGTPIQNALP
                     AYLEAAPAFQADLGARLKANLRALRLALEGPESPHRVLRCDGGWSALLEAPRLLPEEE
                     MAAGLLREEGLQTHPGYFFDMEREAHFMASLILPPGDLAEAARRYKAYFERLLG"
     gene            1777..2670
                     /gene="folD"
                     /locus_tag="PLG14_11080"
     CDS             1777..2670
                     /gene="folD"
                     /locus_tag="PLG14_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOX49718.1"
                     /translation="MGATVIDGKKVAEEIRGEIKAKAALLAKRGVVPGLGVILVGEDP
                     ASLSYVTAKEKACAENGLKSFERRLPASASEAELLAVVGEYNRDPAIHGILVQLPLPR
                     HIDERKVIAAIAPEKDVDGFTPVNVGRMVLDEPCYLPCTPAGVIELIKRSGVRTDGAR
                     AVVVGRSNIVGKPLMNLLVRRSVNATVTVCHTGTRNLAEICREADILVACAGKAGLVT
                     ADMVKPGACVIDVGVNRVEDASAKNGYRLKGDVDYEGASAVAGSITPVPGGVGPMTIT
                     MLLANTVEAAARRLELSEAIA"
     gene            2672..>3320
                     /gene="folE2"
                     /locus_tag="PLG14_11085"
     CDS             2672..>3320
                     /gene="folE2"
                     /locus_tag="PLG14_11085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOX49719.1"
                     /translation="MVDIQSMRDERRIPIQKVGVKGLRYPIKLLDKANAVQHTTALVN
                     LYADLPHDFKGTHMSRFIEVFDAHREDLSMPKFLAMLEKVRESLDAETAFADLHFPYF
                     IEKKAPVSGQAAMMSYDCAYFASVGPKGREFDVAVSVPVQTVCPCSKAISAYGAHNQR
                     GVVRVKLELGPFFWIEDLVALVEGEASSGVYTLLKREDEKSVTERAYENPRFVEDM"
BASE COUNT          474 a         1279 c         1096 g          471 t
ORIGIN      
        1 acttggctgc catggatcgc tccttgcccg tggattggcc gcgcgtcgcg cgacggtagt
       61 agtatacgca caatccgggc cgggggaaag tccgctcgag cgcccccgcg ccgctcgcgc
      121 gctcgcgcgc ccccgtgccg ccccgcgcct ctcgaagcgg ccccgtcccc cgtgctatac
      181 tcgcttcccg tgaagcctcc cttctcgcgg cgggtcccct ggccagccga gccgaacgcc
      241 ctctcctccc tgctcgcgga gcggaggagg gcaggcctgc ccatcctcaa cttcgccgag
      301 tccaatccca cgagggtcgg cctggcccgc gacggcggcg ccctcctagg cgccctcgcc
      361 gtcgaggcct cgctccgcta cgcacccgag ccgcgggggc tcccggggcc ccgcgaggcc
      421 ctcgcggcct tctatgccgg gcgggggact cttcccctcg cccgcgggcc cgccgcctcg
      481 gagaccgccg cctggggaga agctgcctca ggcgcggccg cttcgcggac tgccaatgga
      541 gccgccgcct cagggacagc cggcccgccg gccggcgacg cggaagccgc tggccccctc
      601 ctcggccccg accgcttctt cctctgcgcc tccacctccg aggcctacgg ctggctcttc
      661 aagctcctct gcgagccggg cgaggccgtc ctcgtcccca agccgggcta ccccctcttc
      721 gactacctcg cggggctcga gggcgtggag gcgaggccct accgcctcga gtacttccat
      781 cccaagggct ggaggatcga cctcgagtcc ctggaggccg ccctcgcgga cgggctggcc
      841 agggccgtcg tcctcatcaa cccgaacaac ccgacgggct cctacgtcgg agcggaggag
      901 cgccgcgccg tcgtcgagct cgccgccagg cacggggcgg ccctcgtggt cgacgaagtc
      961 ttcttcggct tccccctgga ggcggagtcc gcctcctcct tcgcgggcga ggaaggcgtc
     1021 ctcaccttcg tcctggacgg cctctccaag ctcctcggcc tcccgcagat gaagctcggc
     1081 tggatagccg cctcgggacc cgctcccgag ctcgcggagg cccttggccg cctcgagatc
     1141 gtcgccgaca cctacctctc ggccgggacg ccgatccaga acgccctgcc cgcctacctc
     1201 gaggccgcgc cggccttcca ggccgacctg ggcgcccgcc tgaaggccaa cttgagggcg
     1261 ctccgcctgg ccctggaggg gcccgaatca ccccaccgcg tcctccgctg cgacggcggc
     1321 tggtcggccc tccttgaggc gccccgcctc ctcccggagg aggagatggc cgccggcctc
     1381 ctccgcgagg agggcctcca gactcacccg ggctacttct tcgacatgga gcgcgaggcc
     1441 catttcatgg cgagcctcat cctgccgccc ggggacctgg ccgaggcggc ccggcgctat
     1501 aaggcctact tcgagcgcct tctaggctag ttgggctcga ggggcgccga caggggccgc
     1561 gcccgccgcg ggctccgcgc cccgggtgcc gcgccgccca gacgcggcgg taccgggccg
     1621 atcgcggccg gtcggcgcgg aggcgcgtcg cttcagacag ccgccggagc gcggcggtcc
     1681 cctccgtttc tccccgccgc gacgcgcggg cggacgttga taaaagcccg cggcctcgct
     1741 atagttggcc atcgtccttc gcgacaaggg ggcaacatgg gcgctacggt catcgacggc
     1801 aagaaagtgg ccgaggagat tcgcggcgag atcaaggcca aggcggccct gctcgcgaag
     1861 aggggcgtcg tcccgggcct gggcgtcatc ctcgtgggag aggatcccgc gagcctctcc
     1921 tacgtgaccg cgaaggagaa ggcctgcgcg gagaacggcc tcaagagctt cgagcggcga
     1981 ctacccgcct ccgcgtcgga ggcggagctc ctggccgtcg tgggcgagta caaccgcgac
     2041 cccgcgatcc acggcatcct cgtccagctg ccccttcccc ggcacatcga cgagcggaag
     2101 gtcatcgccg ccatcgcgcc cgagaaggac gtcgacggct tcacccccgt caacgtgggc
     2161 cgcatggtcc tcgacgagcc ctgctacctg ccctgcacgc cggcgggcgt catcgagctc
     2221 atcaagcgct ccggcgtgag gacggacggc gcccgcgccg tcgtcgtggg ccgctccaac
     2281 atcgtcggga agcccctcat gaacctcctc gtccggcgct ccgtcaacgc gaccgtgacg
     2341 gtctgccaca cgggcacgag gaacctcgcc gagatctgcc gggaggccga catcctggtc
     2401 gcctgcgccg gcaaggccgg cctcgtcacg gccgacatgg tgaagccggg agcctgcgtc
     2461 atcgacgtcg gcgttaaccg cgtggaggac gcgtccgcga agaacggcta ccgcctcaag
     2521 ggcgacgtgg actacgaggg cgcctccgcc gtcgcgggct ccatcacgcc cgtgccgggc
     2581 ggcgtcgggc cgatgacgat cacgatgctc ctcgcgaaca cggtggaggc ggccgccagg
     2641 cgcctcgagc tctccgaggc catcgcctga catggtcgac atccagagca tgcgcgacga
     2701 gcgccgcatc cccatccaga aggtgggggt gaagggcctc cgctacccga tcaagctcct
     2761 ggacaaggcc aacgccgtcc agcacacgac ggccctcgtg aacctctacg ccgacctgcc
     2821 ccacgacttc aagggcaccc acatgagccg cttcatcgag gtcttcgacg cgcaccgcga
     2881 ggacctctcg atgccgaagt tcctcgcgat gctggagaag gtcagggaga gcctggacgc
     2941 ggagaccgcc ttcgccgacc tgcacttccc ctacttcatc gagaagaagg cccccgtgtc
     3001 gggccaggcg gcgatgatgt cctacgactg cgcctacttc gcgagcgtgg gcccgaaggg
     3061 gcgcgagttc gacgtcgccg tctccgtgcc cgtccagacg gtctgcccct gctccaaggc
     3121 gatcagcgcc tacggggccc acaaccagcg gggcgtcgtc cgcgtcaagc tcgagctcgg
     3181 cccgtttttc tggatcgagg acctcgtggc cctcgtcgag ggcgaggcct cgagcggagt
     3241 ctacacgctc ctcaagcgcg aggacgagaa gagcgtcacc gaacgggcct acgagaaccc
     3301 gcgcttcgtc gaggacatgg
//
LOCUS       DAOOSJ010000269         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18758_length_3318_cov_4.490653, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000269 DAOOSJ010000000
VERSION     DAOOSJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18758_length_3318_cov_4.490653"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..922)
                     /locus_tag="PLG14_11090"
     CDS             complement(2..922)
                     /locus_tag="PLG14_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX49720.1"
                     /translation="MMRYGELAAFGTALCWTASAIFFERASKRIGALAVNFYKVLGAF
                     VLLALSGLLLRGMPLPLDAPREAWIYLPISGLIGFVVADYFLFNAYILIGSRLTILFQ
                     PLTPLFAALLGFLILGERMRTAGLAGMALVVAGILVVVVSRNSGGEPRAKGERRPRPT
                     GAAAKGLVFAFASAFFQAVGLIFSKLGLGGYDAISGTQIRVGTAILGFALQALIFGRA
                     RELWVEAPRDRGAMKSTAVGSVFGPFVGVTLSLVALQGTSAGAASTLMGLTPVLIIPP
                     AILVLKQKVKPLEVVGACVAVAGAALFFLL"
     gene            1050..1871
                     /locus_tag="PLG14_11095"
     CDS             1050..1871
                     /locus_tag="PLG14_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOX49721.1"
                     /translation="MAIIAIARELAALGEELAGDLVKLTGYRLVDREFVEKRLGDYGL
                     GPEKRQKYDEKKPGLWASLSQERDDYLHYLKTILYEEAAKGGCIVMGRGGGVILKGLP
                     NLVSVRVVSPIALRVDRIRRLHGCDERRALQILEQSDHDRMGFQKYFFAADWTDAREY
                     GLVVNTGELSPVEAAGLIEAYRKLAATPDREEAGKRRIEELLLGQRVVTEIVYGKRVP
                     IHFLEAEARGRRVVLHGVANTQAAIDSAVSAARGVSGVDEVESAIQVVQEFTVMP"
     gene            2137..2862
                     /gene="rlmB"
                     /locus_tag="PLG14_11100"
     CDS             2137..2862
                     /gene="rlmB"
                     /locus_tag="PLG14_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOX49722.1"
                     /translation="MRKITGFHAIEELLKTGKAEGRLLVSGSGPRIKTILELARKAGL
                     PVVNATNEELSRLAPEHRGVVLETSREEAAGEADLGEFLERAEGAKTSLVLVLDHIED
                     PQNFGAILRSADVFSCDLVIAPNRRAAPLTEAAARASAGALAHVPLALVPNLAEALRR
                     LADSGFWRYAADMGGDSLPKADLPAKLALVMGNEGSGVSRLLRDECDGSLSIPQSGHV
                     DSLNVSAAAAVLLYEYRRRFPAK"
BASE COUNT          538 a         1226 c         1125 g          429 t
ORIGIN      
        1 gctagagcag gaagaagagc gcggcgccgg ccacggcgac gcaggcgccc acgacctcga
       61 ggggcttcac cttctgcttg aggaccagga tggcgggagg gatgatgagg acgggcgtga
      121 ggcccatgag cgtgctcgcg gcgcccgcgg aggtgccctg gagcgcgacg agggagaggg
      181 tgacgccgac gaagggcccg aagaccgagc cgaccgccgt gctcttcatc gcgccgcggt
      241 cgcgcggcgc ctcgacccag agctcccggg cccgcccgaa gatgagcgcc tggagggcga
      301 agccgaggat ggccgtcccc acccggatct gcgtgccgga gatcgcgtcg tagcccccca
      361 ggcccagctt ggagaagatg aggcccacgg cctggaagaa ggcggaggcg aaggcgaaga
      421 ccaggccctt ggcggcggcg cccgtcggcc tgggccgccg ctcgcccttg gcccgcggct
      481 ccccgcccga gttcctggag acgacgacga ccaggatgcc cgcgacgacc agggccatgc
      541 cggccaagcc cgccgtcctc atccgctcgc ccaggatgag gaagcccagg agggcggcga
      601 agaggggcgt gaggggctgg aagaggatcg tgagccggga gccgatcagg atgtaggcgt
      661 tgaagaggaa gtagtccgcg accacgaagc cgatcaggcc ggagatcggg aggtagatcc
      721 aggcctcgcg cggcgcgtcc agggggaggg gcatcccgcg gaggaggagg cccgagaggg
      781 ccaggaggac gaaggcgcct aagaccttgt agaagttcac ggccagggcg ccgatccgct
      841 tcgaggcgcg ctcgaagaag atggcggagg cggtccagca aagggcggtg ccgaaggcgg
      901 cgagctcacc gtagcgcatc atggtgggcc gactatatac gaaaccgccc ggcgtcgtat
      961 aggactcggc ccccgcgcca ggcccccgcg gtttgacagc cccggccggg cggcctaaat
     1021 tcaagatagg aaggaagaag gaggcgatca tggccatcat cgctatcgcc cgcgagctcg
     1081 cggcgctcgg cgaggagctc gccggcgacc tcgtgaagct gaccggatac cggctcgtcg
     1141 accgggagtt cgtggagaag cgcctcggag actacggcct cgggcccgag aagcgccaga
     1201 aatacgacga gaagaagccc ggcctctggg cctcgctctc ccaggagcgg gacgactacc
     1261 tccactacct caagaccatc ctctacgagg aggcggccaa gggcggctgc atcgtcatgg
     1321 gccggggcgg cggcgtcatc ctcaagggcc tgcccaacct cgtctcggtc cgcgtcgtct
     1381 cgcccatcgc cctgcgcgtg gaccggatcc ggcgcctcca cggctgcgac gagcgccgcg
     1441 ccctccagat cctcgagcag agcgaccacg accgcatggg cttccagaag tacttcttcg
     1501 cggccgactg gacggacgcc cgcgagtacg gcctggtcgt caacacgggc gaactctccc
     1561 ccgtggaggc cgccggcctc atcgaggcct accgcaagct ggccgccacg cccgaccgcg
     1621 aggaggcggg caagcgccgc atcgaggagc tcctcctcgg ccagcgcgtc gtcacggaga
     1681 tcgtctacgg caagcgcgtg cccatccact tcctggaggc ggaggcccgc ggcaggcgcg
     1741 tcgtcctcca cggcgtcgcg aacacccagg ccgccatcga ctcggccgtc tccgcggccc
     1801 gcggcgtctc cggcgtggac gaggtggaga gcgccatcca ggtcgtccag gaattcacgg
     1861 tcatgccctg aagcgggggg cgggaggggg aggaaaggcg ggcgcgccgg cgcggccggc
     1921 cccctcccct tccgccgccc aaggctcctg aaagccgccc gcgccgtcgg gctttccgcg
     1981 ggtagcctcc gcgccttccc cgtccggcaa aggcaggacg gcgaaggcgc gcgtcgggct
     2041 acccgctcca atccctcgcg cgacccctcg cgggaagagg cgcctcgccc cgcggggcgc
     2101 ttgcggccct cggccccttc cgcctagtat cggggcatgc gcaagataac gggcttccac
     2161 gccatagaag agctcctcaa gaccggcaag gccgagggcc gcctcctcgt ctccggctcg
     2221 ggcccgcgga tcaagaccat cctcgagctc gcgcggaagg ccggcctgcc cgtcgtcaat
     2281 gccacgaacg aggagctctc ccgcctcgcc cccgagcacc ggggcgtcgt cctcgagacc
     2341 tcgcgcgagg aggcagccgg cgaggccgac ctgggcgagt tcctggagcg ggcggagggc
     2401 gcgaagacct ccctcgtcct cgtcctcgac cacatcgagg acccccagaa cttcggcgcc
     2461 atcctgcgat cggccgacgt cttctcctgc gacctcgtga tcgcccccaa ccgccgcgcc
     2521 gcccccctca ccgaggccgc cgcccgcgcc tcagccggcg ccctcgccca cgtccccctg
     2581 gccctcgtcc ccaacctggc ggaggccctg cgccgcctcg ccgactcggg cttctggcgc
     2641 tacgccgccg acatgggcgg cgacagcctc cccaaggcgg acctccccgc caagctcgcc
     2701 ctcgtgatgg gcaacgaggg ctcgggcgtc tcccgcctcc tccgcgacga gtgcgacggc
     2761 tccctctcca tcccccagtc cggccacgtc gactccctca acgtctccgc cgccgccgcc
     2821 gtcctcctct acgagtaccg ccggcgcttc cccgccaaat agccccgccc gcggcgggtt
     2881 ttcccgggga tcgacgaagc ccgtccgggc ttcgtcgccg ccggagcgca aagcgcgacg
     2941 gcgcggggcc tggctggcgg aaacggactc cacctctccg ctgcgctccg aggggagtcc
     3001 gtttccgcca gccacccgtc gtcctgccta gccgccaata accgcggact ggttgatcgc
     3061 ctgggcgccg cggggccttc gtagcggctt gccccaaaaa actcctgtcg acccggtcga
     3121 gacctttgga ctgccaagcc tcgaaaagcg ctcatgcctg gatcctcccg acccccataa
     3181 agggtttggc ctccaggaag gaaatccagt ttcgcatatg cgaaactgcc gctcttgcta
     3241 tctttcgagc tggaccttag cttttaagta agcggaactc gcgccggcca tgctccaccg
     3301 tacccggcgc cggagctg
//
LOCUS       DAOOSJ010000270         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_18942_length_3297_cov_5.744294, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000270 DAOOSJ010000000
VERSION     DAOOSJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_3297_cov_5.744294"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLG14_11105"
     CDS             complement(<1..787)
                     /locus_tag="PLG14_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX49723.1"
                     /translation="MPFAPDTVPSPNPARVRPGGRARLRGPAAALAALAALSPLCACP
                     LAGPRPGEDSLLVMSYNVKSLFDAVDSGSEFPEFSVAEGRWDEARYKRRLSLLAEVIL
                     AAAPGKRGPDLLCLAEIENRGVLRDLAAGPLKGSGYRETALVEAPGQAFQCGLVSRLP
                     LLSLKAHGLSAGGSEGRYLLEAEVAWEDSGRGSPGPGKPAARPLVVFVCHWKSKVGGA
                     EATEAERREAAALLAGRAAARLAADPACELLACGDFNESPDEYL"
     gene            complement(836..1822)
                     /locus_tag="PLG14_11110"
     CDS             complement(836..1822)
                     /locus_tag="PLG14_11110"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOX49724.1"
                     /translation="MTKESVAERLGSAFESLPDEAARGLPLYRGKVRDVVLGESLVLL
                     VASDRISAFDRILSTVPCKGEVLSRIAAFWFEATSDIVPNHVVAGPSQGPGGLSGTGR
                     ATLVRRAEMLPLEVVVRGYLSGSAWRDYEAGRPVSGIRLPKDLSFNEKFPVPVLTPSS
                     KEAEGHDRPLSREEILSSGLVPPETWLEVERISLALFRRGQELAAKGGLILADTKYEF
                     GLVDGRLTLCDEIHTPDSSRYWWADSYAARFAAADPQRELDKEPFRRWLAERGFMGEG
                     EPPAIPDEVRVETAWRYVQAYEAITGSSFEPLGSDPQAEARALGRIVEERLL"
     gene            complement(1819..2436)
                     /locus_tag="PLG14_11115"
     CDS             complement(1819..2436)
                     /locus_tag="PLG14_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOX49725.1"
                     /translation="MKIDRPVRRFQEEDEEEKKPERRPEDALAERFLKTRTILLTGEI
                     DKDQGERVIRQLLLLESLSDEPITLFIDSPGGDVDAGYAIFDMIRFVKAPVRIVGMGL
                     VASAAALVLLAVPKERRLALPNSHYLIHQPLSGIRGVATEIEIHARELEKTRERINKV
                     IAEETGQPLERVEKDTDRDYWMSAEEALAYGLVSKIGARREELGV"
     gene            2676..>3297
                     /locus_tag="PLG14_11120"
     CDS             2676..>3297
                     /locus_tag="PLG14_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX49726.1"
                     /translation="MMKIAAAKPKSRTAAAAAFLGALALLLLSSCAPEKPAEPPLGAA
                     REELPTATAEGAPVVEAKTATPQAERPWYAARLESLGFYVFPEPVGLDAVELEPLSSA
                     ADAARRLSDLKGKVVLLNFWATWCPPCRLEIPSIAALGKALKGEAFEIAAVSVGEERK
                     TVADFVAAEKLSFPVYLDRSGKLGAEFGARSIPTTYILDREGRIVAG"
BASE COUNT          468 a         1140 c         1224 g          465 t
ORIGIN      
        1 ggaggtattc gtcggggctc tcgttgaagt ccccgcaggc caggagctcg caggccgggt
       61 cggcggccag ccgcgccgcg gcgcggccgg cgagcagggc cgccgcctcg cgccgctcgg
      121 cctccgtggc ctccgcgccg ccgaccttcg atttccagtg gcagacgaag acgacgaggg
      181 gccgcgcggc gggcttgccc ggtcccgggg agccccggcc ggagtcctcc caggcgacct
      241 cggcctcgag gaggtagcgc ccctcgctcc ccccggccga gagcccgtgg gccttgagcg
      301 agagtagggg cagccgcgag acgaggccgc actggaaggc ctggcctggc gcctcgacca
      361 gggccgtctc ccggtagcca gagcccttga gggggccggc cgcgaggtcg cggagcacgc
      421 cccggttctc gatctcggcc aggcagagga ggtcgggccc ccgcttcccg ggcgcggccg
      481 ctagaatcac ctcggcgagg agggataggc gcctcttgta gcgagcctcg tcccatcgcc
      541 cctccgccac ggagaattcc gggaactccg agcccgagtc cacggcgtcg aagagactct
      601 tcacgttgta gctcatcacg aggaggctgt cctcgcccgg ccggggcccg gccagggggc
      661 aggcgcagag cggcgaaagg gccgccaggg ccgcgagggc cgcggccggg ccgcggagcc
      721 gcgcccggcc gcctgggcgg acgcgggcag gattcgggga cggaacggtg tcgggggcaa
      781 agggcatgtc acctcccgcg cctcggccct tgggccgggc gcgggaggtc cggcgctaga
      841 ggaggcgctc ctccacgata cgccccaggg cccgggcctc cgcttgagga tcggagccca
      901 ggggctcgaa ggaggagccg gtgatggcct cgtaggcctg gacgtagcgc caggccgtct
      961 ccacccggac ctcgtcgggg atcgcgggcg gctcgccctc gcccatgaag ccgcgctcgg
     1021 cgagccagcg gcggaagggc tccttgtcga gctcgcgctg gggatcggcc gctgcgaagc
     1081 gggcggcgta ggagtcggcc caccagtagc ggctcgagtc gggcgtgtgg atctcgtcgc
     1141 aaagcgtgag ccgcccgtcc accaggccga actcgtactt cgtgtcggcc aggatgaggc
     1201 cgcccttagc ggccagctcc tggccccgcc ggaagagggc cagggagatc cgctcgacct
     1261 ccagccaggt ctccggaggg acgaggcccg aggagaggat ctcctcgcgg gagaggggcc
     1321 tgtcgtggcc ctcggcctcc ttggaggagg gggtgaggac gggcacgggg aatttctcgt
     1381 tgaaggagag gtccttgggg agccggatgc cggagaccgg cctgcccgcc tcgtagtcac
     1441 gccaggccga gcccgagagg tagccgcgca cgacgacctc cagggggagc atctccgcgc
     1501 ggcggaccag ggtggcgcgg cccgtgccgg aaaggcctcc cggtccctgc gaggggccgg
     1561 cgacgacgtg gttcgggacg atgtcggagg tggcctcgaa ccagaaggcc gctatcctgg
     1621 agaggacctc gcccttgcag ggcacggtgg aaaggatccg gtcgaaggcc gagatgcggt
     1681 cgctcgcgac gaggaggacg aggctttcgc ccaggaccac gtcgcggacc ttgccgcggt
     1741 agagggggag gccgcgggcg gcctcgtcgg gcagggactc gaaggccgag cccaggcgct
     1801 ccgcgacgct ctccttggtc ataccccgag ctcttcccgg cgggcgccga tcttggagac
     1861 gaggccgtag gcgagggcct cctcggcgct catccagtag tcccggtccg tgtccttctc
     1921 gacgcgctcc aggggctggc ccgtctcctc ggctatcacc ttgttgatgc gctcgcgggt
     1981 cttctcgagc tcgcgggcgt ggatctcgat ctccgtcgcc acgcccctga tgccggagag
     2041 gggctggtgg atgaggtagt ggctgttggg aagggcgagg cggcgctcct tgggcacggc
     2101 gaggaggacc agggcggcgg cgctggccac gaggcccatg ccgacgatcc ggaccggcgc
     2161 cttcacgaag cggatcatgt cgaagatcgc gtagcccgcg tcgacgtcgc cgccggggga
     2221 gtcgatgaag agggttatcg gctcgtcgga gaggctctcg aggaggagga gctggcggat
     2281 gacccgctcg ccctggtcct tgtcgatctc cccggtgagg aggatggtgc gggtcttgag
     2341 gaagcgctcg gcgagggcgt cctcgggacg gcgctcgggc ttcttctcct cctcatcctc
     2401 ttcctggaag cgtcggacgg ggcgatctat cttcatggct gctcctttgc ctgttgttcc
     2461 ggataatata ctccgcatcg ccgcctcgcg gccagagtgg ccgtatacta tacgccgcgc
     2521 cgagcctcgc gcaaggccgc gcgggtccgc ccggggcgcc gcctaaagcg ccgcgatagg
     2581 aggcagcgcg cggtaaccgc gggcggaggc cgcgatccgc ggcgcttgtc cccaggacgg
     2641 caatatagta ttttcaagat atacgcttgg aggtcatgat gaagatagcc gccgcgaagc
     2701 ccaaaagccg caccgccgcc gcggcggcct tcctcggggc cctcgccctc ctcctcctct
     2761 cctcctgcgc cccggagaaa cccgcggagc cgcccctggg ggccgcccgc gaggagctgc
     2821 cgacggccac ggccgaaggc gctcccgtcg tggaggccaa gacggccacg ccacaggcgg
     2881 aacgcccctg gtacgcggcc cgcctggaga gcctcggctt ctatgtcttc cccgaacccg
     2941 tgggcctgga cgcggtggag ctcgagcccc tctcctcggc cgcggacgcc gcgcggaggc
     3001 tctccgacct caagggcaag gtggtcctcc tcaacttctg ggccacctgg tgccctccct
     3061 gccgcctgga gatcccctcc atcgccgccc tgggcaaggc cctgaagggc gaggccttcg
     3121 agatcgccgc ggtgagcgtg ggcgaagaga ggaagaccgt cgccgacttc gtcgccgcgg
     3181 agaagctctc cttccccgtc tacctcgacc ggagcggcaa gctcggcgcc gaattcggcg
     3241 cgcggagcat cccgaccacc tacatcctgg accgggaagg ccggatcgtc gccggct
//
LOCUS       DAOOSJ010000271         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19027_length_3286_cov_5.651192, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000271 DAOOSJ010000000
VERSION     DAOOSJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19027_length_3286_cov_5.651192"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLG14_11125"
     CDS             <1..564
                     /locus_tag="PLG14_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOX49727.1"
                     /translation="TFYIELVRGLPMLVILYYIAFVGAPALAAGLNALLAPLIEAGVL
                     AKVEVRGFDFATRAVLALTLGYAAFIAEIFRAGIESVDKGQIEAAEALGLSHGQITRR
                     VILPQALRNVLPALANEFVAVVKDSALVSALGVQDITQLGKVYAASTFKFFETYNAVA
                     FFYLCLTVSLSLLVRALEKRLKRSAAS"
     gene            561..1301
                     /locus_tag="PLG14_11130"
     CDS             561..1301
                     /locus_tag="PLG14_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOX49728.1"
                     /translation="MSAFIEIRGARKRFGAAGEIVALDGVDLDVAKGEVVLVIGPSGS
                     GKSTLLRAVNRLERLDSGSVLVDGVPVTSPRADLRLVREEVGMVFQNFNLFAHLSILE
                     NVCLAPRLVRRLPKAEALARAEALLRRVGLGDKLARFPRQLSGGQQQRAAIARALAMS
                     PKALLFDEPTSALDPEMISEVLDVMKGLAAEGMTMLVVSHEMGFAREAADRVVFMDEG
                     RILETGSPAELFGSPREERTREFLRHVL"
     gene            1374..2324
                     /locus_tag="PLG14_11135"
     CDS             1374..2324
                     /locus_tag="PLG14_11135"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOX49729.1"
                     /translation="MRETDFRLKDQEGVELFVRRWEPGSGKPKAVVQLEHGVAEHSGR
                     YAATAEALCAAGYAVYADDHRGHGYSAEATGGLGRLGPGGWEAVVRDLGLLSDRVAEE
                     MPGLPLFLVGHSWGSYLAQDYAQRWGHRLAGLVLVGTSGGQPFLVKGVGPWVGRLFVL
                     FLGRDRPSRIADAFAFKPYNRPYLPSPTDSDSDWISRDIEEVRSFVDDPACAFPLTTG
                     LSLEMSLAFRRLWRPGMEARIPKSLPILLMSGTDDASNGRLKYLLPFAERLRKRGHED
                     LTLKLYEGARHCILHETNRGEVKADLVAWLDSRFGKRKAG"
     gene            2438..3283
                     /locus_tag="PLG14_11140"
     CDS             2438..3283
                     /locus_tag="PLG14_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HOX49730.1"
                     /translation="MVFQSDFGVADGAVSAMYGVAYSVDPCLRVFDLTHDIPQYDVWE
                     ASYRLVQTIQYWPAGTVFVSVVDPGVGSSRRSVAVRTRGGQIVVTPDNGSLTHVKLML
                     GVAEARELSERGNRLSGSELSYTFHGRDVFAYTGARLAAGRVPFDGLGPALDPASLVE
                     LPHVPAVREGDAVTGTIDVLDVRFGSLWSNIPHGLFEGLGVPYGGRVEVSIRNDTREL
                     YRNTMAYAKSFAEVNVGEPLVYINSILNLAVAINQGSFARAYNIGTGLSWKLAVRKAP
                     RVVYE"
BASE COUNT          458 a         1236 c         1085 g          507 t
ORIGIN      
        1 accttctaca tcgagctcgt gcgcggcctc ccgatgctcg tcatcctcta ctacatcgcc
       61 ttcgtcgggg cgccggccct ggcggcaggc ctcaacgccc tccttgcgcc cctcatcgag
      121 gccggcgtcc tcgccaaggt cgaggtgcgg ggcttcgact tcgcgacgcg ggccgtcctg
      181 gccctcaccc tgggctacgc cgccttcatc gcggagatct tccgcgcggg catcgagagc
      241 gtcgacaagg gccagatcga ggccgcggag gccctgggcc tgtcgcacgg ccagataacg
      301 cgccgcgtga tcctgcccca ggcgctgcgc aacgtcctcc cggccctggc gaacgagttc
      361 gtcgcggtgg tgaaggactc cgccctggtc tccgccctgg gagtccagga catcacccag
      421 ctcggcaagg tctacgcggc ctccaccttc aagttcttcg agacctacaa cgcggtggcc
      481 ttcttctacc tctgcctcac ggtctcgctc tccctcctcg tccgggcgct cgagaagcgc
      541 ctgaagcgga gcgcggcctc gtgagcgcct tcatcgagat ccgcggcgcg aggaagcgct
      601 tcggcgccgc gggcgagata gtcgccctgg acggcgtcga cctggacgtg gccaagggcg
      661 aggtcgtcct cgtcatcgga ccctcgggct cggggaagag caccctcctg cgcgcggtga
      721 accgcctgga gcgcctcgac tcgggctcgg tcctcgtgga cggcgtcccg gtcacctcgc
      781 cccgcgccga cctccgcctc gtccgcgagg aggtcggcat ggtcttccag aacttcaacc
      841 tcttcgccca cctctccatc ctcgagaacg tctgcctggc gccccgcctc gtcaggcggc
      901 tcccgaaggc ggaggccctc gcgcgagccg aggccctctt gaggcgcgtc ggcctcgggg
      961 acaagctcgc gcgcttcccg cgccagctct ccggtggcca gcagcagcgc gcggcgatcg
     1021 cccgggccct cgcgatgagc ccgaaggccc tcctcttcga cgagccgacg agcgccctcg
     1081 atcccgagat gatcagcgag gtcctggacg tcatgaaggg cctggccgcg gagggcatga
     1141 cgatgctcgt cgtctcccac gagatgggct tcgcccgcga ggcggccgac cgcgtcgtct
     1201 tcatggacga ggggaggatc ctcgagacgg gcagcccggc cgagctcttc ggaagcccca
     1261 gggaggagcg gacccgcgag ttcctccgcc acgtccttta ggccccttcc ctggccgagg
     1321 gcggccgcct ccactacact tttactcggg atagacgagc aagggggcgc gccgtgcggg
     1381 agaccgattt caggctgaag gaccaagagg gcgtcgagct cttcgtcagg cgctgggagc
     1441 ccggctccgg gaagcccaag gcggtagtcc agctcgagca cggcgtcgcc gagcactccg
     1501 gacgctacgc cgcgacggcg gaggccctgt gcgccgccgg ctacgcggtc tacgccgacg
     1561 accatcgggg ccacggctac agcgccgagg cgacgggcgg actgggacgg ctcggccccg
     1621 gcggctggga agccgtcgtc cgcgacctcg gcctcctctc cgaccgcgtc gcggaggaga
     1681 tgccgggcct gcccctcttc ctcgtcgggc actcctgggg aagctacctg gcccaggact
     1741 acgcccaacg ctggggccat cgcctcgcgg gcctcgtcct cgtcggcacg tcgggaggcc
     1801 agcccttcct cgtcaagggc gtcggcccct gggtggggcg gctcttcgtc ctcttcctgg
     1861 ggcgggaccg cccgagccgg atcgcggacg ccttcgcctt caagccctac aacaggccct
     1921 acctcccctc cccgacggat tcggactcgg actggatctc ccgcgacatc gaggaggtca
     1981 ggtccttcgt ggacgacccc gcctgcgcct tccccctcac gacgggactc tccctggaga
     2041 tgagcctggc cttccggcgg ctctggcggc ccgggatgga ggcgcgcatc ccgaagagcc
     2101 tccccatcct cctcatgtcg ggcacggacg acgcgtcgaa cgggcggctc aagtacctcc
     2161 tgcccttcgc cgagcgcctg cggaagcgcg gccacgagga cctgacgctc aagctctacg
     2221 agggcgcccg gcactgcatc ctccacgaga ccaaccgcgg ggaggtcaag gccgacctcg
     2281 tcgcctggct ggactcccgc ttcgggaagc gcaaggcggg atagcgccgc gctgcgtcga
     2341 tccgggtcgc cccgccctcg ttgacgcgcg gccgcggctc tgctaccaag gaggggcgga
     2401 gccagcctcc gcgaggaggc cgtccctgtc cgacgcgttg gtgttccaga gcgacttcgg
     2461 cgtcgcggac ggcgccgtct ccgccatgta cggcgtcgcc tattcggtcg atccctgcct
     2521 cagggtcttc gacctcaccc acgacatccc gcagtacgat gtctgggagg cctcctaccg
     2581 cctggtccag accatccagt actggcccgc gggcacggtc ttcgtctcgg tggtggaccc
     2641 cggcgtcggg agctcgaggc ggagcgtcgc ggtcaggacc aggggcggcc agatcgtcgt
     2701 cacgcccgac aacggcagcc tcacccacgt gaagctcatg ctcggcgtcg cggaggcgcg
     2761 ggagctctcg gagcggggca acaggctctc gggctccgag ctctcctaca ccttccacgg
     2821 ccgcgacgtc ttcgcctaca cgggagcgcg cctggccgcg ggccgcgtcc ccttcgacgg
     2881 gctcgggccg gccctggacc ccgcgtccct cgtcgagctg ccacacgtcc cggccgtccg
     2941 ggagggcgac gcggtgaccg gcaccatcga cgtcctggac gtgcgcttcg ggagcctctg
     3001 gtcgaacata ccccacggcc tcttcgaggg cctgggcgtg ccctacggcg gccgcgtgga
     3061 ggtctccatc aggaacgaca cgcgggagct ctaccggaac acgatggcct acgcgaagtc
     3121 cttcgccgag gtcaacgtgg gcgagcccct cgtctacatc aactcgatcc tcaacctggc
     3181 cgtcgccatc aaccaaggct ccttcgcgcg cgcctacaac atcggcaccg gcctctcctg
     3241 gaagctggcc gtgcggaagg cgccgcgcgt cgtctacgaa tgaaga
//
LOCUS       DAOOSJ010000272         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19123_length_3275_cov_5.643168, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000272 DAOOSJ010000000
VERSION     DAOOSJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19123_length_3275_cov_5.643168"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..788)
                     /locus_tag="PLG14_11145"
     CDS             complement(15..788)
                     /locus_tag="PLG14_11145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX49731.1"
                     /translation="MSEYRYDEVSRIYDLVRDADRRPLELMGRKAALGPASRVLDLGC
                     GTGSHALLLAAMYGSRITGVEPSEGMRERARAKAAKLAPEAEWLPGSASSIPAADGSF
                     DLVYMTDVVHHVPDLEAMFEEAARVLAPGGLFAVASQSHAQIAARPVARFFPETVAAD
                     RARYPDSGRLAAAAAPAGLAFLGDEAYGEGEPVVLDESFLELAERKGYSVLAAIGEEA
                     YRKGLASLRAALAESAREGRPLRLPAAGGTVHWFAKAAR"
     gene            complement(785..1543)
                     /locus_tag="PLG14_11150"
     CDS             complement(785..1543)
                     /locus_tag="PLG14_11150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX49732.1"
                     /translation="MIVELEELSANAWPGLRSALVGGWVARSAGGYTRRANCVLPLYG
                     AGGPLSPRIGRCEALAAAWGIRPVFKIADPKGLAELDVELEGRGYAVEAPTTVMALAS
                     LPHAGAPPAARPGLEACLAKEAGEEWYEAFLGFSGIEGQKAMLARAIMSSIAPDRRFV
                     LLKEGGEAVACALLVLEDGWAGVFDVVVRRDRRGRGLGRDLMGLVLSEAARSGARRSY
                     LQVMDDNAPAIGLYESLGYAPAYGYYYRAKGEAL"
     gene            complement(1540..2421)
                     /locus_tag="PLG14_11155"
     CDS             complement(1540..2421)
                     /locus_tag="PLG14_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX49733.1"
                     /translation="MDLRLGYYPVLDAALGLRQLHEGLRFAPFGPAMETLRERLASEA
                     AAIEAVGAASDGWLGVLESLLAPESAGLAGAEEGLVRLESDADAAGALVAKALRAVVA
                     PEAARRSRALVASVEAIGRGLAAAGPWDYLAGLSDRLRRAGPGELLYKIKPAFRFREE
                     EVERLVVTPSLLATRRLTFWRREGTFLFFVGGEGEPEADEAPDGVLLGALALADRTRL
                     RMLRRLSASPCGNAEMADFLGVNPSTASRHFKLFKDAGLVELRGAEGARLEYELAPEG
                     LDAALRAIAEYIKGGRT"
     gene            2571..2987
                     /locus_tag="PLG14_11160"
     CDS             2571..2987
                     /locus_tag="PLG14_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX49734.1"
                     /translation="MKHEIHCSWKGGMAFEADVLGHAVRMDADKESGGADSGARPKPL
                     LLAALSGCTGMDVVSILMKMRQPLSYFDMQVEGELAEEHPKRYNSMTLVYRFRDSDGL
                     DRQKVLDAVTLSLEKYCGVIATLRDAVEFSWRIDYL"
     gene            2966..>3275
                     /locus_tag="PLG14_11165"
     CDS             2966..>3275
                     /locus_tag="PLG14_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49735.1"
                     /translation="MADRLPLSWPLRAAPRASSRKGRLAAALLLLLAAPAFGAAPASA
                     NAPVVRLAPLAAPAPANLSANAPAAQLVSAAAPADAARTGAPLVLVLQSYQRDYAWTV
                     G"
BASE COUNT          445 a         1228 c         1172 g          430 t
ORIGIN      
        1 ggccgcggcc cgccctagcg ggcggccttt gcgaaccagt ggaccgtgcc gccggcggcg
       61 gggagcctga ggggccgccc ctcgcgggcg gactccgcca aggcggcgcg gagcgaggcg
      121 agtcccttgc ggtaggcctc ctcgccgatg gccgcgagca ccgagtagcc cttccgctcc
      181 gcgagctcga ggaagctttc gtccaggacg acgggctccc cctcgccgta ggcctcgtcg
      241 cccaggaagg cgagcccggc gggagccgcc gcggccgcga gccgtcccga gtccgggtaa
      301 cgcgcccggt cggccgcgac cgtctccggg aagaagcgcg cgaccgggcg ggcggctatc
      361 tgggcgtggc tctggctcgc gacggcgaag aggccgccgg gagcgaggac ccgcgccgcc
      421 tcctcgaaca tcgcctccag gtccgggacg tggtggacga cgtccgtcat gtagacgagg
      481 tcgaaggagc cgtccgccgc ggggatggag gaggccgagc ccgggagcca ctcggcctcc
      541 ggcgcgagct ttgcggcctt tgcgcgggcc ctctcgcgca tgccctcgga gggctcgacc
      601 ccggtgatcc gcgagccgta catcgcggca aggaggagtg cgtggctccc cgtgccgcag
      661 cccaggtcga ggacccgcga ggcggggccg agggcggcct ttctccccat gagctcgagg
      721 gggcgccggt cggcgtcccg cacgaggtcg tagatcctcg agacttcgtc gtagcggtat
      781 tcgctcatag cgcctctccc ttcgcgcggt agtagtagcc gtaggccggg gcgtagccga
      841 ggctttcgta gagcccgatc gcgggcgcgt tgtcgtccat cacctggagg taggagcgcc
      901 gggcgcctga gcgggccgcc tccgagagga cgaggcccat gaggtcccgg ccgaggcccc
      961 ggccccgccg gtcccgccgc acgacgacat cgaagacgcc cgcccagccg tcctccagga
     1021 cgaggagggc gcaggcgacg gcctcgccgc cctccttgag gaggacgaag cggcggtcgg
     1081 gcgcgatgga cgacatgatg gcgcgggcga gcatggcctt ttgcccctcg atgccggaga
     1141 agccgaggaa ggcttcgtac cactcctcgc cggcctcctt tgccaggcag gcctcgaggc
     1201 cggggcgggc cgcgggcggc gcgccggcgt gcgggaggga ggccagggcc atcaccgtcg
     1261 tcggcgcctc caccgcgtag ccccttccct cgagctcaac gtccagctcg gcgagcccct
     1321 tcgggtcggc gatcttgaag acgggcctga tgccccaggc cgccgccagg gcctcgcagc
     1381 gcccgatccg cggcgaaagg ggcccgcccg ccccgtagag cggcagcacg cagttggctc
     1441 ggcgcgtgta gccgccggcc gagcgcgcga cccagccgcc gaccagggcc gagcgcaggc
     1501 ccggccaggc gttggccgaa agctcctcca gttcgacgat catgtccttc ctccttttat
     1561 gtattcggct atcgcgcgga gggccgcgtc gagcccctcg ggcgcgagct cgtactcgag
     1621 ccgcgccccc tccgccccgc ggagctccac gagccccgcg tccttgaaga gcttgaagtg
     1681 cctggacgcg gtcgagggat tgacccccag gaagtccgcc atctccgcgt tgccgcaggg
     1741 cgaggcggag agccgcctga gcatcctgag cctcgtgcgg tcggccaggg ccagggcgcc
     1801 gagaaggacg ccgtcgggcg cctcgtcggc ctccggctcc ccctcgccgc cgacgaagaa
     1861 gaggaaggtc ccctcccgcc gccagaaggt caggcgcctc gtcgccagga gggagggcgt
     1921 gaccacgagt cgctcgacct cctcctccct gaagcggaag gcgggcttga tcttgtagag
     1981 gagttcgccc ggccccgcgc gcctcagcct gtcggagagc ccggcgaggt agtcccaggg
     2041 gccggccgcc gcgaggccgc ggccgatggc ctccaccgac gcgacgaggg cccgggaacg
     2101 cctcgccgcc tcgggggcga cgacggcgcg tagggccttc gccacgaggg ccccggccgc
     2161 gtccgcatcg gactcgagcc gcaccaggcc ctcctccgct cccgcgagcc ccgccgactc
     2221 gggagcgagg aggctctcga ggacgccgag ccagccgtcg ctggcggccc cgacggcctc
     2281 tatggccgcg gcctccgagg ccaggcgctc gcggagcgtc tccatcgcgg gaccgaaggg
     2341 cgcgaagcgc aggccctcgt ggagctggcg caggcccagg gccgcgtcca gcacggggta
     2401 gtagccgagg cgcaggtcca tgcgtccctc cttgtctggc caatgttgat gcaccgttgc
     2461 gcaagcatgc aacgaatcgc gattcctgtc aagaagcccc cgccgagcct cccccgattg
     2521 gcatggcgcc ggagcgcccc gtcgcttata gtcctagcta agggggaagc atgaaacacg
     2581 agatccattg ctcctggaag ggcggcatgg ccttcgaggc cgacgtcctc ggccacgcgg
     2641 tgcgcatgga cgccgacaag gagtcgggcg gggcggactc cggcgcgagg ccgaagcccc
     2701 tcctcctcgc ggccctctcc ggctgcaccg gcatggacgt cgtctccatc ctcatgaaga
     2761 tgcgccagcc cctgagctac ttcgacatgc aggtcgaggg cgagctcgcg gaggagcatc
     2821 cgaagcgcta caactccatg accctggtct accgcttcag ggactccgac ggcttggacc
     2881 gccagaaagt gctggacgcc gtgaccctct ccctggagaa gtactgcggc gtgatcgcca
     2941 ccctccgcga cgccgtcgaa ttctcgtggc ggatcgacta cctctgagct ggccgctccg
     3001 cgcggcgccg cgggcctcct cccggaaggg gcggctcgcg gcggccctcc tccttctcct
     3061 cgcggcgccg gccttcggcg ccgctcccgc gtcggccaac gcgcccgtgg tccggcttgc
     3121 gcccctcgcc gcgcccgcgc cggccaacct gtcggccaac gcgccggcgg cccagcttgt
     3181 gtcagccgcc gcgccggcgg acgccgcccg gaccggggcg cccctcgtcc tcgtcctcca
     3241 gtcctaccag cgcgactacg cgtggacggt cggca
//
LOCUS       DAOOSJ010000273         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19369_length_3248_cov_4.984654, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000273 DAOOSJ010000000
VERSION     DAOOSJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19369_length_3248_cov_4.984654"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..886
                     /locus_tag="PLG14_11170"
     CDS             191..886
                     /locus_tag="PLG14_11170"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOX49736.1"
                     /translation="MPREYCYRCRRPKELCLCPAKPPMETRTRIVLLMHPMEWKREKC
                     TTGRLTCLNLANSEILQGIAFDGHPRVRELVDDPGNYPVLLYPGKGAMDITEGGFPKA
                     ELGGRRLVVFLIDSTWSCARSVVRASPGLMRLPRLAFTPREKSRWVIKRQPADYCLST
                     LEATHELLLALEAAGLDEYPDKGRLLAAFAAMQAVQVERAAANGSPRYLERGDRSRDQ
                     SRGGSCDGTADGD"
     gene            complement(938..1738)
                     /locus_tag="PLG14_11175"
     CDS             complement(938..1738)
                     /locus_tag="PLG14_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX49737.1"
                     /translation="MSHAIQGDGYETPEGIPRRASELLSLGTRWSPYFQFFGVMFRER
                     ALALRGLYDDEAWAESSLEILERLEGCGARFSISGLSRLSSFEGPAVIVSNHMSTLET
                     LILPGLVAPRKPCTFVVKVELLHGPIWGPIMRSRDPIAVTRKDARRDLETVLGEGRRL
                     LSSGRSVVVFPQATRRDVFSRAEFNSIGAKLASRAGVPLLPVAVKTDYWGNSPLFRGF
                     GPVRRDRRVHIEFGEPMPVSGRGKAEHEASVDFIESRLRAWGAPIAET"
     gene            complement(1735..>3248)
                     /locus_tag="PLG14_11180"
     CDS             complement(1735..>3248)
                     /locus_tag="PLG14_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49738.1"
                     /translation="AGAAEKPGADEPAGRPGVAGPAGDPGAWTRGALRRRIRNLAALR
                     DPATYLSFDRYTRAVLDLNRALKAVSAPYGAEAGLADYRELSRSPLRRSDLVAAAREP
                     ESSPFYPLFRRRLQAELSGSPGEEVGISVCFLSQALPAFAIAGLVRALAPKARIILGG
                     GLVGSWLAQGSLEPRDRFEGFVDALVAGKGEEELGRLLGLGGDSTESGGVAEGGGAAE
                     SGGAAEGGTSWSLPDFSGFEDLRYLSPAKVIPYNFSSGCPWRRCSFCPEKAEGWPYAG
                     IAAEAARRELASLAELHRPGLLHFTDNEISPLHLRALAESPPGAPWYGFARFTPLLAD
                     PGFCARLAAAGCRMLQLGLESGDQAVLDALGKGTRLEEIDRALEALAGAGVGVYLYVL
                     FGTPAEDRDAALRTRDFVAARAERIDFLNVAVFNMPASSPEARAYPSRSFYEGELSLY
                     SEFSHPRGWNRGEVRAFLAEDFEASPGIKSILRRNPPVFTSSHAPFFLRGGKE"
BASE COUNT          508 a         1127 c         1195 g          418 t
ORIGIN      
        1 ggccttcgcc cgggagaagg gcttcgcggg cgtgacctgc acctgcggct tcgacggcgc
       61 ctcgcggcga aaggaagtca ggcgcaagat agccgagctc accgggggct cgagcgagca
      121 gaagaggaac ctcttcgcct ccatgtcgcg ggtgaacgag gagtacctcg cctagcgcgg
      181 ggccgtggcc atgccgaggg aatactgcta ccgctgccgc cgccccaagg agctctgcct
      241 ctgccccgcg aagccgccca tggagacgag gacgaggatc gtcctcctca tgcaccccat
      301 ggagtggaag cgcgagaagt gcacgacggg caggctcacc tgcctcaacc tcgcgaacag
      361 cgagatcctc cagggcatcg ccttcgacgg ccacccgcgc gtccgcgagc tcgtcgacga
      421 tcccgggaac taccccgtcc tcctctaccc gggcaagggc gcgatggaca tcacggaggg
      481 cggctttccg aaggccgagc tcggcggaag gcggctcgtg gtcttcctca tcgactccac
      541 ctggtcctgc gcgagaagcg tcgtccgcgc cagccccggc ctcatgcggc tgccccgcct
      601 cgcgttcacg ccccgggaga agagccgctg ggtcatcaag cgccagccag ccgactactg
      661 cctctcgacc ctggaggcga cgcacgagct cctcctggcc ctggaggccg cgggcctcga
      721 cgagtacccc gacaagggca ggctccttgc ggccttcgcg gcgatgcagg cagtccaggt
      781 ggagagggcg gcggcgaacg gctccccgcg ctacctggag cgcggcgacc ggtcccgcga
      841 ccagagccga ggcgggtcct gcgacggtac ggcggacggc gactgatgga gcggcgcgcc
      901 gcgcctcgcc gagcctagtc ctccgcgaca gcgcggctca agtttccgct atcggagctc
      961 cccaggcgcg cagccgcgac tcgatgaagt ccaccgaggc ctcgtgctcg gccttgcccc
     1021 gtccggagac gggcatcggc tcgccgaact cgatgtggac gcggcggtcc cggcgcacgg
     1081 ggccgaagcc gcggaagagc ggggagttgc cccagtagtc ggtcttcacg gccacgggga
     1141 ggaggggcac gcccgcccgc gaggcgagct tggccccgat gctgttgaac tccgcgcggg
     1201 agaagacgtc cctcctggtc gcctgcggga agacgacgac gctgcggccg gaggagagga
     1261 ggcgccggcc ctctccgagg acggtctcca ggtcgcgcct cgcgtccttc ctcgtcacgg
     1321 ctatgggatc gcgggagcgc atgataggcc cccagatcgg cccatggagg agctcgacct
     1381 tcacgacgaa ggtgcagggc ttgcgcggcg cgacgaggcc ggggaggatc agggtctcca
     1441 gggtgctcat gtggttggac acgatcaccg ccggcccctc gaaggaggag agccgggaga
     1501 gccccgagat cgagaagcgc gccccgcagc cctcgagccg ctccaggatc tcgagcgagc
     1561 tctccgccca ggcctcgtcg tcgtagaggc cgcggagcgc gagggcccgc tcccggaaca
     1621 tgaccccgaa gaactggaag taggggctcc atctcgtgcc gagggagagg agctccgaag
     1681 cccgcctggg aatcccctcg ggagtctcgt agccgtcgcc ctggatcgca tgactcattc
     1741 cttgcctcct cggagaaaaa agggagcgtg gctggacgtg aacacgggag ggttccgcct
     1801 caggatggac ttgatcccgg gcgacgcctc gaagtcctcg gccaggaagg cgcggacctc
     1861 gccgcggttc cagccgcgcg gatggctgaa ctcgctgtag agcgagagct ctccctcgta
     1921 gaaagaccgc gagggatagg ccctggcctc ggggctcgag gcgggcatgt tgaagacggc
     1981 gacgttgagg aagtctatcc tctccgcgcg cgcggccacg aaatcccgcg tgcgcaaggc
     2041 cgcgtcgcgg tcctccgccg gcgtgccgaa gaggacgtag aggtagacgc cgacgccggc
     2101 cccggccaag gcctcgaggg ccctgtcgat ctcctcgagc ctcgtgccct tgcccagggc
     2161 gtcgaggacc gcctggtccc ccgactcgag tcccagctgg agcatgcggc agcccgcggc
     2221 cgcgaggcgg gcgcagaatc ccggatcggc gagaaggggc gtgaagcggg cgaagccgta
     2281 ccagggcgcc cccggcgggc tctccgcgag ggcgcggagg tggagggggg agatctcgtt
     2341 gtcggtgaag tggaggagcc cgggccggtg gagctccgcg agggaggcga gctcccggcg
     2401 ggcggcctca gccgcgatgc cggcgtaggg ccagccctcc gccttctcag ggcagaagct
     2461 gcacctccgc caggggcagc ccgaggaaaa gttgtacgga atcaccttgg ccggggagag
     2521 gtagcgcaag tcctcgaatc cggagaagtc gggcagggac caggaggtgc cgccttccgc
     2581 cgcgccgccg ctttccgccg cgccgccgcc ctccgctacg ccgccgcttt ccgtcgaatc
     2641 gccccccagg ccgaggaggc ggccgagctc ctcctcgccc ttgcccgcga ccagggcgtc
     2701 gacgaagccc tcgaagcggt cccggggctc aaggctcccc tgggccagcc aggagccgac
     2761 gaggccgccg cccaggatga tcctggcctt gggcgcgagg gcgcggacta ggccggctat
     2821 cgcgaaggcc ggcagggcct gggagaggaa gcagacggag atccccacct cctcgccggg
     2881 gctgcccgag agctcggcct ggagccgccg gcggaagagg gggtagaagg gactcgactc
     2941 cggctcgcgg gccgcggcga cgaggtcgga tctccgcaag ggggagcgcg agagctcgcg
     3001 gtagtcggcg agtccggcct cggcgccata gggggccgag acggccttga gcgcgcggtt
     3061 caggtcgagg acggcccggg tgtagcggtc gaagctcaag taggtggcgg gatcccgcag
     3121 ggccgcgagg ttgcggatcc gccggcgaag cgcgcctctc gtccaggctc cggggtcgcc
     3181 cgccggcccc gcgactccgg gccttccggc cggctcatcg gctccgggtt tctcggccgc
     3241 gccggccg
//
LOCUS       DAOOSJ010000274         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19430_length_3242_cov_4.921243, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000274 DAOOSJ010000000
VERSION     DAOOSJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19430_length_3242_cov_4.921243"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLG14_11185"
     CDS             <1..774
                     /locus_tag="PLG14_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOX49739.1"
                     /translation="RLLAGMESIAGGDYSGRLELKEVEEFGLVAGAFNAMMEGVERRD
                     ARIKADLGEKVILLKEVHHRVKNNLQIMASLMNLASRSIKDPEDSLVFKASQDRVHSM
                     ALVHELLYQSEDLSSIDMADYVKSLVFYLRDAYPAPRLGIKVEVEPLELPLEKALPCG
                     LLLNELLTNSLKYATGGPGEALIEVRLRSGDATGRVVLEVGDRGPGLPSGAPTGKEES
                     LGLSLVESLSKQLEGELSFSPAALGENPPGLLARLEFPR"
     gene            complement(834..1841)
                     /locus_tag="PLG14_11190"
     CDS             complement(834..1841)
                     /locus_tag="PLG14_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX49740.1"
                     /translation="MRLLFIGGTGNLSYETSLRALAKGYELFHLNRGRRVDRSLEEVT
                     TLYADIRDPEAARAALGELDFDAVVDFIAYTPEQVRLDLELFEGRTSQYVFISSASAY
                     RKPPASHVISESTPLSNPFWSYSRDKIACELLLKEEWARTGFPVTVVRPSHTYGLGWI
                     PTAWSSSDFTVPARMLQGKEIVVHGDGQSLWTLTHARDFAVGLVGLLGNPAAIGETVQ
                     ITGDEALTWEAIHGAIAAALGVTPRIVHVPSDFIAEVDPEMGEHFLGDKSWSALFDCS
                     KLKRLVPEFRTTVPFAEGVRESVAWCLADPDRQRINPDIDASIERVLAAWRRAMAAAQ
                     K"
     gene            1951..>3242
                     /locus_tag="PLG14_11195"
     CDS             1951..>3242
                     /locus_tag="PLG14_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49741.1"
                     /translation="MPSSRAHMARALAATILSLAALAAAEAWEVHPLISYPMLESLPE
                     AAAAGEVRAEALEDFVLAAEDELAALLAAEEAWARKNVPEYPPLPEAMAFRATGDGTD
                     ARLRLLRSLRMTPIAKIPLYVQKKPGEEVREGRRLEPVDLTYLKDIAWMEGLPYESIL
                     AGENVSALAVCATASDEPDLGLDIGLYEDNGSAWGKDYGMGAQPFGNPNLEYGSQAPI
                     HMGLYQEAAVLYKFGPFLRKTLPEWRIRQCKALAELAFRTGHDYWGWRFAGWGLHYLA
                     DLCQPYHARILPGVSTARALWINALSMLGNGKPYAKAVQLVSNRHMALERYGRELLEK
                     AYREGGADAPLFAALGRNEAVPAWEDSFPRTRISEAASKAAAKTDRLVAKSFPKRLVS
                     DPKVELASAPELYDLMGAVASEKGAKSAEALEAAMAGLL"
BASE COUNT          530 a         1127 c         1096 g          489 t
ORIGIN      
        1 aggctcctcg cgggcatgga gtcgatagcg ggcggggact actcggggcg gctcgagctc
       61 aaggaggtcg aggagttcgg cctggtcgcg ggggccttca acgcgatgat ggagggcgtg
      121 gagcggcgcg acgcgaggat caaggccgac cttggcgaga aggtcatcct cctcaaggag
      181 gtccaccacc gcgtcaagaa caacctgcag atcatggcga gcctcatgaa cctggcctcg
      241 cgctcgatca aggaccccga ggacagcctg gtcttcaagg cgagccagga ccgcgtccac
      301 tccatggccc tggtccacga gctcctctac cagtcggagg acctctcctc catcgacatg
      361 gccgactacg tgaagagcct cgtcttctat ctccgggacg cctaccccgc gccgcggctc
      421 ggcatcaagg tcgaggtcga gcccctggag ctgccgctgg agaaggccct gccctgcgga
      481 ctcctcctca acgagctgtt gacgaacagc ctcaagtacg cgaccggcgg gccgggcgag
      541 gccctcatcg aggtccgcct gcgctccggg gacgcgacgg gccgagtcgt cctggaggtg
      601 ggcgacaggg ggccgggcct gccctcggga gcgccgacgg gcaaggagga gagccttggc
      661 ctctcgctcg tggaaagcct ctcgaagcag ctcgaaggcg agctctcctt ctcgccggcg
      721 gccctgggcg agaatccgcc cggactcctc gctcggcttg agttcccgcg ctgaccgagc
      781 ctcgcgttcg cgcggcggcc gtcgcgcgcc cgccttttac tgcggcgacg gcctcacttc
      841 tgagcggcgg ccatcgcgcg gcgccaggcc gccaggaccc gctcgatcga cgcgtcgatg
      901 tccgggttga tccgctggcg gtccggatca gctaggcacc aggccaccga ctccctcacg
      961 ccttcagcga aaggcacggt cgtcctgaac tcgggcacga ggcgcttgag cttggagcag
     1021 tcgaagagcg ccgaccagct cttgtcgccc aggaagtgct cgcccatctc ggggtcgacc
     1081 tccgcgatga agtccgaggg cacgtggact atccgcggcg tgacgcccag ggcggccgcg
     1141 atggccccgt ggatagcctc ccaggtgagg gcctcgtccc cggtgatctg cacggtttcg
     1201 cctatcgcgg cgggattgcc gaggagcccc acgaggccga cggcgaagtc ccgggcgtgg
     1261 gtcagggtcc agagcgactg gccgtcgccg tggacgacga tctccttgcc ctggagcatg
     1321 cgcgcgggca cggtgaagtc cgaggatgac caggccgtcg ggatccagcc caggccgtag
     1381 gtgtgggagg gccgcacgac ggtgacgggg aagcccgtcc tggcccattc ctccttgagg
     1441 aggagctcgc aggctatctt gtcccgcgag taggaccaga agggattgga gaggggcgtc
     1501 gattcggaga tcacgtggct cgccggcggc ttgcggtagg ccgaggcgct cgagatgaag
     1561 acgtactggg aggtcctgcc ctcgaaaagc tcgaggtcga gcctgacctg ctcgggcgtg
     1621 taggcgatga agtcgacgac ggcgtcgaag tcaagctcgc ccagggcggc cctcgccgcc
     1681 tcggggtcgc ggatgtccgc gtagagggtg gtcacctcct ccaggctccg gtcgacccgc
     1741 cggccccggt tcaggtggaa gagctcgtag cccttggcta gggcgcggag cgaggtttcg
     1801 taggagaggt tcccggtacc gccaatgaag agcaagcgca tcttccggcc tcctatgccc
     1861 ctaagtatag cgcctcaggc ggtttcttgt tcgcggttcc ggcgaatcgt gctaggcttc
     1921 gatggatcgc actcgcccca aggagtcgcc atgccaagct cccgagccca tatggcccgc
     1981 gccctcgcgg caaccatcct ctcgctcgcc gccctcgccg ccgcggaggc ctgggaggtc
     2041 caccctctca tctcctaccc catgctcgag tccctgcccg aagccgctgc ggccggcgag
     2101 gtccgggcgg aggcgctgga ggacttcgtc ctcgccgcgg aggacgagct cgcggccctc
     2161 ctcgccgcgg aggaggcctg ggcccggaag aacgtccccg agtatccgcc cctccccgag
     2221 gccatggctt tcagggcgac gggcgacggg accgacgcca ggctccgcct tcttcggtcg
     2281 ctccggatga cgccgatcgc gaaaatcccc ctctacgtcc agaagaagcc cggagaggag
     2341 gtccgcgagg gaaggagact cgagcccgtc gacctcacct acctgaagga cattgcctgg
     2401 atggagggcc tcccctacga gtccatcctg gccggagaaa acgtctccgc gctggccgtc
     2461 tgcgccaccg cgagcgacga gcccgacctg gggctggaca tcggcctcta cgaggacaac
     2521 gggagcgcct ggggcaagga ctacggcatg ggcgcccagc ccttcggcaa tcccaacctg
     2581 gagtacgggt cgcaggcgcc gatccacatg ggcctctacc aagaggcggc cgtgctctac
     2641 aaattcggac ccttcctcag gaagaccctg cccgagtggc ggatccgcca gtgcaaggcc
     2701 ctggccgagc tcgccttcag gaccggccac gactactggg gctggcgctt cgcgggctgg
     2761 ggcctccact acctggccga cctatgccag ccctaccacg cgaggatcct ccccggcgtc
     2821 tccacggcca gggcgctctg gatcaacgcc ctctccatgc tcgggaacgg aaagccctac
     2881 gccaaggccg tccagctcgt ctccaaccgc cacatggccc tggagcgcta cggcagggag
     2941 ctcctggaaa aagcctaccg cgagggcgga gccgatgccc ctctcttcgc ggccctcgga
     3001 cggaacgagg cagtccccgc ctgggaggac tcctttcccc gaacgaggat atccgaggcg
     3061 gcctccaagg ccgcggcgaa gaccgaccgc ctggtcgcga agagcttccc taagcgcctc
     3121 gtatccgacc ccaaggtcga gctcgcgtcc gcgcccgagc tgtacgacct catgggcgcg
     3181 gtagcctcgg agaaaggcgc caagtcggcc gaagccctgg aggcggcgat ggccggactc
     3241 ct
//
LOCUS       DAOOSJ010000275         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19495_length_3233_cov_4.475456, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000275 DAOOSJ010000000
VERSION     DAOOSJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_3233_cov_4.475456"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..1996)
                     /gene="pyrB"
                     /locus_tag="PLG14_11200"
     CDS             complement(404..1996)
                     /gene="pyrB"
                     /locus_tag="PLG14_11200"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOX49742.1"
                     /translation="MARKGPFKGRTVAVVNDLGADEQLYLYRKARELKEAALAGRDLS
                     AFRLDERDYQVYLIFMEDSTRTRESFRNAAEFLGSRVNVFDAATSSFNKNESIADTVK
                     MLSGYASDSAFVIRSKLEGVCRALEGVIGSYADQSGRPRPAFVNAGDGKHEHPTQEFL
                     DEFSFLEQLEWKRDRIHLALTGDLYHGRTVHSKVDGLSVFKEVEVDLVAPELLGMPAH
                     YVERMRRKGYEVRVFESLDEYLAQKKVAPIWYFTRLQLERMGDAVLERADSLRGDLTF
                     RRDMLGKLPEGARFYHPLPRDRAKPTNPTFLDELPLNGWDAQSANGYWTRIVLLGMLS
                     GLLGSDFDGASRPIEGFDEDFVVEAPVSEHPAREYKVGIKPVEEGIVIDHVASGRPID
                     EIWSNIDAARRILGLNVRSSHGVFHNNRGSFKGIISLPDVTSFGERDLKKLAAIAPGC
                     TLNLVRGGRVVKKYRLAMPPRIYAFDEISCKNPNCVSAPAHQEGLSPEFLRKTPAAGE
                     ASTTFVCKYCEREHAFREIWDV"
     gene            complement(2053..>3233)
                     /locus_tag="PLG14_11205"
     CDS             complement(2053..>3233)
                     /locus_tag="PLG14_11205"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOX49743.1"
                     /translation="IGSRYKAVVAGNVSSENAGLALRYDQWPGKSRILIPLSAGARPG
                     SLGAVDTGAVSDKQVTESLRAEPGKGVEDRQALTDLKEREVEEKKAAAEEKKGEAAKA
                     EADLAAEKARLAEADAKLEAAKKEEAAAKAEADKAAAAGDAQEAAVARSEAEKAAAER
                     EAAAAEKAAAEKAVAAKEETAAAKTEEAAKAEAAVAAKEAEVAADRKEVSADQKAAIA
                     EEVAAKQKGEAAGVYLFEVVDSGYPFARIVFVDADSGKLIRASALNSIRARSVVDAGD
                     SYVAVAGREGGTGAVRLVRVEKASLMAVAEGKTDLFPESLVWKVGDSYYAVAKAEGGK
                     WRLARFDASLAEVARCAVDVNPYTFLAEAAGGLAVQAAGGGFAVLSKDKLEKVKELKP
                     "
BASE COUNT          414 a         1095 c         1213 g          511 t
ORIGIN      
        1 ggtatggggg gtatcccccc atccagaggg caacacggaa tgagtggggg tagcgtagaa
       61 gcccggagcc cgcgcctctt ggcgcgggcg aggacttcgg agcgaggggg agccgcgtca
      121 tccgcggcgc cgcgcccgcg aggaagcccg cgaagcgcgg cgcgcctccc cccgaagagg
      181 cgacgctcct ggaagcgggc cctcgtgggt caaacccgcc cgcgctttcc actcgggcgg
      241 gcgggacggc gggtggcgcg gggaagcggc cggccttcgg agcggagcgg agaagggccg
      301 gccgcttccc cgcgccagga cccgccccga cgggccttgg agagcccgac cggggagcgc
      361 gccgcgcgat ggactctagg ccgggcgctc cccccttcgc ctcctacacg tcccagatct
      421 cgcggaaggc gtgctccctc tcgcagtact tgcagacgaa ggtcgtggag gcctcgccgg
      481 cggcgggggt cttgcggagg aactccggcg agaggccctc ctggtgggcc ggggcggaga
      541 cgcagttcgg gttcttgcag gagatctcgt cgaaggcgta gatgcgcggg ggcatcgcga
      601 ggcggtactt cttgacgacg cggccgccgc ggacgaggtt cagggtgcag ccgggggcga
      661 tggcggccaa cttcttgagg tcgcgctcgc cgaagctcgt gacgtcgggc agggagatga
      721 tgcccttgaa gctgccgcgg ttgttgtgga agacgccgtg gctggagcgg acgttgaggc
      781 cgaggatgcg ccgggccgcg tcgatgttgg accagatctc gtcgatgggc cggccggagg
      841 cgacgtggtc gatgacgatg ccctcctcca cgggcttgat gccgaccttg tactcgcggg
      901 ccgggtgctc ggagacgggg gcctccacga cgaagtcctc gtcgaagccc tctatggggc
      961 gcgacgcgcc gtcgaagtcg gagcccagga ggccggagag catgccgagg aggacgatgc
     1021 gggtccagta gccgttggcc gactgggcgt cccagccgtt gagggggagc tcgtccagga
     1081 aggtcgggtt cgtgggcttg gcgcggtcgc gcgggagggg gtggtagaag cgcgcgccct
     1141 cggggagctt cccgagcatg tcgcgcctga aggtgaggtc gccgcgcagg gagtccgcgc
     1201 gctcgaggac ggcgtcgccc atcctctcga gctggaggcg ggtgaagtac cagatcggcg
     1261 cgaccttctt ctgggcgagg tactcgtcca gggactcgaa cacgcggacc tcgtagccct
     1321 tccgcctcat gcgctcgacg tagtgggcgg gcatgcccag gagctccggc gcgacgaggt
     1381 cgacctccac ctccttgaag acggagaggc cgtccacctt ggagtggacg gtgcggccgt
     1441 ggtagaggtc gcccgtgagg gcgaggtgga tgcggtcgcg cttccactcg agctgctcga
     1501 ggaaggagaa ctcgtcgagg aactcctggg tggggtgctc gtgcttgccg tcgcccgcgt
     1561 tgacgaaggc ggggcggggc cgccccgact ggtcggcgta ggagccgatg acgccctcca
     1621 gggcgcggca gacgccctcc agcttggagc ggatgacgaa ggcggagtcg gaggcgtagc
     1681 ctgagagcat cttcacggtg tcggcgatgg actcgttctt gttgaaggag gaggtcgccg
     1741 cgtcgaagac gttgacgcgg gagccgagga actccgcggc gttgcggaag gactcgcggg
     1801 tccgggtcga gtcctccatg aagatgaggt agacctggta gtcgcgctcg tccaggcgga
     1861 aggcggagag gtcgcggccc gccagggccg cctccttgag ctcgcgcgcc ttccggtaga
     1921 gatacagctg ctcgtccgcg cccaggtcgt tcacgaccgc gacggtccgc cccttgaagg
     1981 gccctttcct cgccatggct tcctcctcct cttatgaaaa aggcgggcac gaggcccgcc
     2041 ttggtcaggc tgtcagggct tgagctcctt gaccttctcc agcttgtcct tggacaggac
     2101 ggcgaagccg ccgcccgcgg cctggaccgc caggcctccc gccgcctcgg cgaggaaggt
     2161 gtaggggttc acgtcgaccg cgcatcgcgc gacctccgcg agggaggcgt cgaagcgcgc
     2221 gaggcgccac ttcccgccct ccgccttggc gaccgcgtag taggagtcgc cgaccttcca
     2281 gacgaggctc tccgggaaga ggtcggtctt cccctcggcg accgccatga ggctcgcctt
     2341 ctccacgcgg acgaggcgca cggcgcccgt gccgccctcg cgtccggcga cggcgacgta
     2401 ggagtccccg gcgtccacga cggagcgggc gcggatggag ttgagggcgg aggcgcggat
     2461 gagcttgcct gagtccgcgt cgacgaagac gatccgcgcg aaggggtagc ccgagtcgac
     2521 gacctcgaag aggtagacgc ccgcggcctc gcccttctgc ttggcggcga cctcctcggc
     2581 gatcgcggcc ttctggtcgg cgctgacctc cttgcggtcg gcggcgacct ccgcctcctt
     2641 ggcggcgacg gcggcctcgg ccttcgctgc ctcctccgtc ttcgcggcgg ccgtctcctc
     2701 cttcgcggcg acggccttct ccgcggcggc cttctccgcg gcggcggcct cgcgctcggc
     2761 ggcggccttc tccgcctcgc tccgcgcgac ggcggcctcc tgggcgtcgc ccgcggcggc
     2821 ggccttgtcg gcctcagcct tggcggccgc ctcttccttc ttcgcggcct cgagtttcgc
     2881 gtccgcctcc gcgaggcggg ccttctccgc ggccaggtcg gcctcggcct tcgcggcctc
     2941 gcccttcttc tcctcggccg cggccttctt ctcctcgacc tcgcgctcct tgaggtccgt
     3001 gagggcctgc cggtcctcca cgcccttccc gggctcggcg cggaggctct ccgtcacctg
     3061 cttgtcggag acggcgcccg tgtccacggc cccgagggag ccgggcctgg ccccggcgga
     3121 gagggggatg aggatgcgcg acttcccggg ccactggtcg tagcgcaggg cgaggccggc
     3181 gttctccgaa ctcacgttgc ccgccaccac ggccttgtag cggctcccga tgt
//
LOCUS       DAOOSJ010000276         3223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19584_length_3223_cov_4.754735, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000276 DAOOSJ010000000
VERSION     DAOOSJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19584_length_3223_cov_4.754735"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLG14_11210"
     CDS             complement(<1..454)
                     /locus_tag="PLG14_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOX49744.1"
                     /translation="MTSDIHVGTVFKSGNDVVVVQRLLGQKSGRGGMVRKMRVKNIVT
                     GQTSDLALDMGEKFEDVHLDVLAMKLSYVDGETYVFMEQTTFEQYEISKEDLGDNAGY
                     LTPEDEFEVEITFHEGKPVGVVLPIAVERTITYCEPGVKGDTSGKTLKP"
     gene            complement(552..1742)
                     /gene="earP"
                     /locus_tag="PLG14_11215"
     CDS             complement(552..1742)
                     /gene="earP"
                     /locus_tag="PLG14_11215"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOX49745.1"
                     /translation="MTIDLLCKVVDNYGDIGVVYRLAKALSGLDPGLSLRLVVDNLAS
                     FRALAPEVDPARPLQRLRGWTVAAWDQPAAAPGAAAESARELYRRERPRFVIECFACG
                     RPEWLEEILFDPEDPGKRLVVNLEYLTAEDYAREFHLMPSATRSGLVRKSFFMPGFAP
                     GTGGLILDPGFREARARFLDPATRGAARLKALKAAGFEPEPGEGERFWVSVFSYERDY
                     GRIVADLAAYGRERPLLALVASGRSEACFRKAWEKAGRPFPARFLPFLPQEAWDEILL
                     ASDFSIVRGEESLARAALAGRPFLWHAYVQEGAHQLVKVRALLSRLKACFAEPDYTLL
                     EELFLAFNDRLADGPDRAGRESLLPLLERESALERGFEDFSRRLFELGDLAGHLLTFL
                     RDFV"
     gene            complement(1885..2754)
                     /locus_tag="PLG14_11220"
     CDS             complement(1885..2754)
                     /locus_tag="PLG14_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX49746.1"
                     /translation="MKAPFDGIELMNSGHSGCPGCGATLAMKLALRALGEDTALVIPA
                     CCWAIIAGGFPQTALKVPVLHTGFATTAAAASGLRAALDARDMRSTTVVGWAGDGGTF
                     DIGFQALSAAAERGEDFIYVCYDNEAYMNTGVQRSSSTPFGTRTTTTPGAGWKKTEKK
                     NIMEIMAAHRIPYAATASIAYPEDLEAKLRKAAAVRTGLRFIHLYASCPTGWGFPAQY
                     AVKTARMAVDCNVFPLYEVEDGLRYTVNSKGEERVADYLNFQGRFRHLSEAEIGHIQG
                     RVDEEWARLLSRA"
     gene            complement(2751..>3223)
                     /locus_tag="PLG14_11225"
     CDS             complement(2751..>3223)
                     /locus_tag="PLG14_11225"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49747.1"
                     /translation="LLKLKMFRPFPAEALARALAPKAGLAGTGGAAGATGAGGASGGA
                     SGGASARGASGATGPEAPSTEAGRAAAAAPRKLAVVDRNNSAGVGGIFAQEIRAALSG
                     REGAPEVHSYVAGLGGRDITTATLEKIYRETLAAGRPGAGSVWVDCDEELAKSS"
BASE COUNT          452 a         1064 c         1222 g          485 t
ORIGIN      
        1 cgggcttcag ggtcttgccg gaggtgtcgc ccttgacgcc gggctcacag taggtgatcg
       61 tgcgctcgac ggcgatgggg aggacgacgc cgacgggctt gccctcgtgg aaggtgatct
      121 ccacctcgaa ctcgtcctcg ggggtgaggt agccggcgtt gtcgcccagg tcttccttgg
      181 agatctcgta ctgctcgaag gtggtctgct ccatgaagac gtaggtctcg ccgtcgacgt
      241 aggagagctt catcgccagg acgtcgaggt ggacgtcctc gaacttctcg cccatgtcca
      301 gggcgaggtc gctcgtctgg ccggtgacga tgttcttgac gcgcatcttg cgcaccatgc
      361 cgccgcggcc gctcttctgg cccaggaggc gctggacgac gacgacgtcg ttgccggact
      421 tgaagacggt gcccacgtga atatcggagg tcatcagcat aaagaaattc ctcgcatagg
      481 gatggattgg ggccctgttt tccgcggttt ggcgccaggc caagggacgg gcccgctcgg
      541 tcggggccat tctacacgaa atcgcgcaag aaagtcagta ggtgtccggc caggtcgccg
      601 agttcgaaaa gcctgcggga aaagtcctcg aagccccgct cgagcgcgct ttcgcgctcg
      661 aggaggggga ggaggctctc ccgcccggcc ctgtccgggc cgtcagcgag gcggtcgttg
      721 aaggcgagga agagctcctc gaggagggtg tagtcgggct cggcgaagca ggccttaagg
      781 cgcgagagga gggcgcggac cttcacgagc tggtgggctc cctcctggac gtaggcgtgc
      841 cagaggaagg gcctgcctgc gagggcggcg cgggccaggg attcctcgcc gcggacgatg
      901 gagaaatcgc ttgcgaggag gatctcgtcc caggcctcct gggggaggaa ggggaggaag
      961 cgggcgggga agggccggcc ggccttttcc caggccttcc gaaagcaggc ctcgctccgc
     1021 ccgctcgcca cgagggcgag gaggggccgc tccctgccgt aggcggcgag gtcggccacg
     1081 atccgcccgt agtcgcgctc gtagctgaag acggagaccc agaaacgctc cccctcgccg
     1141 ggctcgggct cgaagcccgc ggccttcagg gccttgaggc gggcggctcc cctggtcgcg
     1201 gggtcgagga agcgcgcccg ggcttccctg aagcccgggt cgaggatgag gccgcccgtg
     1261 cccggggcga agccgggcat gaagaagctc ttcctgacga ggcccgacct ggtcgcggag
     1321 ggcatcaggt ggaactcccg ggcgtagtcc tccgccgtga ggtactcgag gttgacgacg
     1381 agccgcttcc cggggtcctc cgggtcgaag aggatctcct ccaaccactc gggacggccg
     1441 caggcgaagc actcgatgac gaagcgcggc cgctcgcggc ggtagagctc gcgcgcggac
     1501 tccgccgcgg cgccgggcgc cgccgcgggc tggtcccagg cggcgacggt ccagcccctg
     1561 aggcgctgga ggggccgcgc gggatcgacc tccggggcga gggcgcggaa tgaggcgagg
     1621 ttgtcgacga cgaggcggag ggagaggccg ggatcgaggc cggagagggc cttcgcgagc
     1681 ctgtagacga cgccgatgtc gccgtagttg tcgacgacct tgcagaggag gtcgatcgtc
     1741 acgacgccac ccggcgggcg ggccgccgcg gcgccgggcc gaacgaaggc gcggcgcgcg
     1801 gcgcgaggaa cgcgccggcc gccgcggcgg cgcgggcctc gttgcctagg cgcgccctcg
     1861 cagcggcggc cggcggccgc atgcctaggc gcgcgacagg agccttgccc attcctcgtc
     1921 cacgcggccc tggatgtggc cgatctccgc ctccgagagg tggcggaagc ggccctgaaa
     1981 gttgaggtag tcggcgaccc gctcctcgcc cttggagttc acggtgtagc ggaggccgtc
     2041 ctccacctcg tagaggggga agacgttgca gtccacggcc atgcgggcgg tcttcaccgc
     2101 gtactgggcg ggaaagcccc agcccgtggg gcagcttgcg tagaggtgga tgaagcggag
     2161 acccgtcctg acggccgcag ccttcctgag cttcgcctcc aggtcctcgg gataggcgat
     2221 gctcgcggtc gcggcgtagg ggatgcggtg ggccgccatg atctccatga tgttcttctt
     2281 ctcggtcttt ttccagcccg ccccgggggt cgtcgtcgtc ctcgtcccga agggggtcga
     2341 ggaggagcgc tggacgcccg tgttcatgta ggcctcgttg tcgtagcaga cgtagatgaa
     2401 gtcctcgccc cgctcggcgg cggcggagag ggcctggaag ccgatgtcga aggtgccgcc
     2461 gtccccggcc cagccgacga ccgtcgtgga gcgcatgtcc cgggcgtcca gggccgcgcg
     2521 gaggccgctc gcggccgccg ccgtcgtggc gaagcccgtg tggaggacgg ggaccttgag
     2581 ggcggtctgg gggaagccgc ccgcgatgat ggcccagcag caggccggga tcacgagggc
     2641 cgtgtcctcg cccagggcgc gcagggcgag cttcatcgcc agggtcgccc cgcagccggg
     2701 acagccggag tggccggagt tcatgagttc gatgccgtcg aatggtgcct tcatgatgat
     2761 ttcgcgagct cctcgtcgca gtctacccag acgcttccgg ccccgggcct gccggccgcc
     2821 agggtctccc ggtatatctt ctcgagggtg gcggtcgtga tgtcgcggcc gcccaggccc
     2881 gcgacgtagc tgtggacctc cggcgcccct tcgcggcccg agagggcggc cctgatctcc
     2941 tgggcgaaga tgcccccgac gccggccgag ttgttgcggt ccacgacggc gagcttcctc
     3001 ggcgccgccg cggccgccct gccggcctcg gtgctcggcg cctccgggcc cgtggcgcca
     3061 gatgcgccgc gcgcactcgc gccgccactc gcgccgccac tcgcgccgcc cgcgcccgtg
     3121 gcgccggccg cgccgcccgt gcccgcaagg ccggccttgg gcgcgagagc cctggccagg
     3181 gcctccgcgg ggaagggcct gaacatcttg agcttgagga ggc
//
LOCUS       DAOOSJ010000277         3185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_19942_length_3185_cov_4.693930, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000277 DAOOSJ010000000
VERSION     DAOOSJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_3185_cov_4.693930"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLG14_11230"
     CDS             complement(<1..1182)
                     /locus_tag="PLG14_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX49748.1"
                     /translation="MDLERAYSRIPDRRYADFRAFLEAAGGRGVGEGGRLKPALRWRP
                     ECSEEERVVSFPELRAKAEGLGSFLLGRGLGRGGRVAILSENRPEWCVSYLAVVAAGL
                     VAVPLDASLDEAGLLRNLAAARCQALFVSGRLAKRFPALCAAAGLVLDFDLEPGKGED
                     GRVSFAEAAAAPLSAPLPKAGELGPKAPAVIFFTSGTTGVAKGITLSHGAVIANVNAS
                     RMSLYVDEDDVFVALLPLHHTYATTCSFLSGIEAGCTVVVVDRIAPSAVLRAIREGGV
                     TFLIGVPLLFDKVRGGIEAEFSKLPSFPRALLRGALSLSRFSALRLGLPLGRLLFRPV
                     REKAGLGTVRLAVSGGGPLAPATADFFDALGINLVQGYGMSENGPLISVNLPKRKDNR
                     SV"
     gene            complement(1192..2967)
                     /gene="pheT"
                     /locus_tag="PLG14_11235"
     CDS             complement(1192..2967)
                     /gene="pheT"
                     /locus_tag="PLG14_11235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOX49749.1"
                     /translation="MPKIEVNESLFFNLLGRACGPEELERILPSAKAELDGWDLASGA
                     GAAGGAGGGAAKPGDAGAVDRSIKIELNDTNRPDLWSTAGLARQLRLAEGGKRPEYPF
                     FSWEGAPRPASKKVVVEESVKGVRPYLAGFVVKGKAITDAVLKDLIQTQEKLAWNFGR
                     KRRTVSMGLYRSGSIQWPVTYKAVDPKTTRFVPLQETRSMSLREILAEHPKGKEYAFI
                     LEKEPLHPLLVDAKGGVLSYPPIINSADLGAVQVGDTELFVELTGTDLPSVVLSASIA
                     ACDLSDMGYSVEPVEVDYAYDTAFGRKLVTPFYFQKPVSVEAARVARLLGKPFSVQAV
                     ADAAVRMGSGVDIHGQYVAVRPAEYRNDFLHAVDVVEDVMIGLGMDAFAPERPRDFTI
                     GRLSPIEAFSRKAKNAMTGLGYQEMIYNYLGSRRDYIDKMGLDPEALVRISNPMSENF
                     EYVRNSPLPSLFNSESVSSQAAYPHRLFEVGKVAVKDKAENYGVATRQYIGVLSCHAA
                     ADFNEIAAQVATLLYYLGRDFTVADPAHAGGEDPRFIPGRQAAVLYKGKRAGIYGETH
                     PGLLEAWGIAMPCAAAELDLDAFLA"
     gene            complement(2991..>3185)
                     /gene="pheS"
                     /locus_tag="PLG14_11240"
     CDS             complement(2991..>3185)
                     /gene="pheS"
                     /locus_tag="PLG14_11240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967638.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOX49750.1"
                     /translation="LGWFELGGAGIFRPEVTEPFGVKVPVLAWGIGIDRMALMALGLN
                     DLRELFSSDVESVRLRRAKI"
BASE COUNT          484 a          982 c         1259 g          460 t
ORIGIN      
        1 gaccgagcgg ttgtccttgc gcttgggcag gttgacggag atgagggggc cgttctcgct
       61 catcccgtag ccctggacca ggtttatccc gagggcgtcg aagaagtcgg ccgtcgcggg
      121 cgcgaggggg ccgccgccgg agaccgcgag gcggaccgtg cccaggccgg ccttctccct
      181 gacgggcctg aagaggagac ggccgagggg caggccgagc ctgagggcgg agaaccggga
      241 gagggacagg gcgccgcgga ggagggcgcg ggggaaggag gggagcttcg agaactcggc
      301 ctcgatgccg ccgcgcacct tgtcgaagag gaggggcacg ccgatgagga aggtgacgcc
      361 gccctcgcgg atcgcgcgga gcaccgcgga gggggctatc ctgtcgacga ccacgacggt
      421 gcagcccgcc tcgatccccg agaggaagga gcaggtcgtc gcgtaggtgt ggtggagggg
      481 gaggagggcg acgaagacgt cgtcctcgtc cacgtagagg gacatgcgcg aggcgttcac
      541 gttggcgatg acggcgccgt gggagagggt gatgcccttc gccacgcccg tcgtgcccga
      601 ggtgaagaag atgacggccg gcgccttggg cccgagctcc cccgccttag ggaggggggc
      661 cgagagggga gcggcggcgg cctccgcgaa ggagacgcgg ccgtcctcgc ccttcccggg
      721 ctccaggtcg aagtccagga cgaggccggc cgccgcgcag agggcgggga agcgtttcgc
      781 gagccggccg gagacgaaga gggcctggca gcgcgccgcg gccaggttcc gcaggaggcc
      841 cgcctcgtcg agcgaggcgt ccagggggac ggcgacgagg ccggccgcga cgacggcgag
      901 gtaggagacg caccactcgg ggcggttctc cgagaggatg gcgactcggc cgccccgtcc
      961 cagtccccgt ccgaggagga aggagcccag gccctcggcc ttggccctga gctcgggaaa
     1021 gctcacgacc cgctcttcct ccgagcactc gggccgccag cgcagggcgg gcttcaggcg
     1081 cccgccctct ccgacgcccc gtccgcctgc cgcctccagg aaggcgcgga agtccgcgta
     1141 gcggcggtcg gggatgcggg aataggcgcg ttccaaatcc acggtcgctc cttaggcgag
     1201 gaatgcgtcg aggtcgagct cggcggcggc gcagggcatc gcgatgcccc aggcctcgag
     1261 aaggccgggg tgggtctcgc cgtagatccc cgcccgcttc cccttgtaga ggacggcggc
     1321 ctgccttccc gggatgaagc ggggatcctc cccgcctgcg tgcgcggggt cggcgaccgt
     1381 gaagtcgcgg ccgaggtaat agaggagggt cgccacctgg gcggcgatct cgttgaagtc
     1441 ggcggcggcg tggcaggaga ggacgccgat gtactgcctc gtcgcgaccc cgtagttctc
     1501 cgccttgtcc ttgaccgcca ccttgccgac ctcgaagagg cggtgcggat aggcggcctg
     1561 gctggagacg gattcggagt tgaagaggga gggaaggggc gagttgcgca cgtactcgaa
     1621 gttctcggac atggggttcg agatgcggac gagggcctcg gggtcgaggc ccatcttgtc
     1681 gatgtagtcg cggcggctcc cgaggtagtt gtagatcatc tcctggtagc cgaggcccgt
     1741 catcgcgttc ttggccttgc gggagaaggc ctctatcggg gagaggcggc cgatcgtgaa
     1801 gtcgcggggc cgctcgggcg cgaaggcgtc catgccgagg ccgatcatca cgtcctccac
     1861 gacgtcgacc gcgtggagga agtcgttccg gtactccgcg gggcggacgg cgacgtactg
     1921 gccgtggatg tcgacgcccg agcccatgcg gactgcggcg tcggcgacgg cctggacgga
     1981 gaagggcttc ccgaggaggc gggcgacgcg cgcggcctcc acggagacgg gcttctggaa
     2041 gtagaagggc gtcacgagct tcctgccgaa ggccgtgtca taggcgtagt cgacctcgac
     2101 gggctcgacc gagtagccca tgtccgagag gtcgcaggcc gcgatggagg cggagaggac
     2161 gacggagggg aggtcggtgc ccgtgagctc gacgaagagc tcggtgtcgc cgacctggac
     2221 ggcgcccagg tcggcggagt tgatgatcgg cggataggag aggacgccgc ccttcgcgtc
     2281 gacgaggagg ggatggaggg gctccttctc gaggatgaag gcgtactcct tgcccttcgg
     2341 gtgctcggcc aggatctcgc ggaggctcat ggagcgcgtc tcctggaggg ggacgaagcg
     2401 cgtggtcttg gggtcgacgg ccttgtaggt cacgggccac tggatggagc cgctccggta
     2461 gagacccatg gagacggtgc ggcgcttgcg gccgaagttc caggcgagct tctcctgggt
     2521 ctggatcagg tccttgagga cggcgtccgt gatcgccttg cccttgacca cgaagccggc
     2581 caagtagggg cggacgccct ttaccgactc ctcgacgacg accttcttgg aggcgggccg
     2641 gggggcgccc tcccaggaga agaaggggta ctcggggcgc ttcccgccct ccgcgagcct
     2701 gagctggcgg gcgaggccgg ccgtggacca gaggtcgggg cggttcgtgt cgttgagctc
     2761 gatcttgatg ctccggtcca cggcgcccgc gtcgccgggc ttggccgcgc cgccgccggc
     2821 cccgccggcg gcgccagctc cgctggcgag gtcccagccg tcgagctcgg ccttggccga
     2881 ggggaggatg cgctcgagct cctcggggcc gcaggcgcgg ccgagaaggt tgaagaagag
     2941 gctctcgttg acttctatct tgggcatgtc gctatcctta gggtctttcg ctagatcttg
     3001 gcgcggcgga gccgcacgct ctctacgtcg gagctgaaga gctcgcggag gtcgttgagg
     3061 ccgagggcca tgagggccat ccggtcgatg ccgatgcccc aggccaggac gggcaccttg
     3121 accccgaagg gctcggtgac ctcgggcctg aagatgccgg cgccgccgag ctcgaaccag
     3181 ccgag
//
LOCUS       DAOOSJ010000278         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_20007_length_3177_cov_6.610186, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000278 DAOOSJ010000000
VERSION     DAOOSJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20007_length_3177_cov_6.610186"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLG14_11245"
     CDS             complement(<1..538)
                     /locus_tag="PLG14_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOX49751.1"
                     /translation="MVNAYIKQVLDDLKARYPWETEFLQAAEEVLESIAPLIEAEPKY
                     KQQRILERITEPERTIMFRVPWIDDKGEYHVNRGYRCQFNSAIGPYKGGLRFHPKVQL
                     STLKFLGFEQIFKNSLTGLPMGGGKGGSDFDPNGKSDNEIMRFCQSFMTELVKHVGAD
                     TDVPAGDIGVGGREVGYMF"
     gene            complement(843..2915)
                     /locus_tag="PLG14_11250"
     CDS             complement(843..2915)
                     /locus_tag="PLG14_11250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49752.1"
                     /translation="MQPKPSSPSCRRARALALAAVLALLAAAAPGAQPASEARALAAA
                     AEAKLFAEDVYGAVEDYLAAVARNPSYAEALLGLAECYYELEEYDQALSYAKKAAPLM
                     RGEPVLMDLEGFIRLGLGDLAGARSLFSAVLAGRPNDLDARFGLALLDLAAGKKTDAK
                     LRLEDSLRLSPENARALLSLALVASDQGRAEEARVLVEKALRFHGEEPRVQYTAARLA
                     AAEGDRERAVFHARNALDASPAYAEARRLLASLMYEAASYEEAAALMREAVARDRKDA
                     AAWFTLGLAQAGAGRGAEALYSLRQAAALKPDDEVARIALENLVVDSGPFEDPSREPY
                     AEWHFARGAEFEDRSLFDQALFEYRRGLRIYPYSKKGRVLYAELLRKRGLPGKQLAEL
                     KFLAGIGKADTAVSDAIEIYSSLLEDSVSRAWSLDQYSLPKRPYRLAVFTLPDRGEGV
                     HTAGSAVLLRYLKDLLAGSSRLSVLPLAAEARNPAEAFRRAREAEADYYLVFRARETE
                     RDLELSGELRVARTGSPATVCRAYRSGNDRVKNTASRLASLVEGALEPKGSVLRRSQD
                     KALADLGSSDGIKAGDRLVVLKRGSLDVKPEGLGAAYAPSAVVGELLVTRVDEELCEG
                     SLKSAGFFDTINVGDELVLAPPVPPAAAGVTDGAAAPAKAGQAPAKPAVPLEPEWPGL
                     FTAVRKLR"
     gene            complement(2917..>3177)
                     /locus_tag="PLG14_11255"
     CDS             complement(2917..>3177)
                     /locus_tag="PLG14_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOX49753.1"
                     /translation="EDLSSLGEGVWLCDARADLDDLNEELGLELPADDFETLGGYIFD
                     LFGRIPARGERIASGGLEFVVQEMDGNRITLIKVQRRPPEED"
BASE COUNT          456 a         1009 c         1221 g          491 t
ORIGIN      
        1 cgaacatgta gccgacctcg cggccgccca cgccgatgtc gcccgcgggc acgtccgtgt
       61 cggcgcccac gtgcttcacg agctcggtca tgaagctctg gcagaagcgc atgatctcgt
      121 tgtccgactt gccgttcggg tcgaagtcgg agccgccctt gccgccgccc atggggaggc
      181 cggtgaggga gttcttgaag atctgctcga agccgaggaa cttgagggtg gagagctgga
      241 ctttcgggtg gaagcggagg ccgcccttgt aggggccgat ggcggagttg aactggcagc
      301 ggtagccgcg gttgacgtgg tactcgccct tgtcgtcgat ccagggaacg cggaacatga
      361 tggtgcgctc gggctcggtg atgcgctcga ggatcctctg ctgcttgtac ttgggctccg
      421 cctcgatgag gggggcgatg gactcgagga cttcctcggc cgcctggagg aactcggtct
      481 cccagggata gcgggccttg aggtcgtcga ggacctgctt gatataggcg ttcacaattt
      541 cctccttcta tggatcggga aatataacgg atattttcgg gcgatctcag gatcgctcgt
      601 ccatgagtgt atacctagtt atcgattcag gcaagggtga aaatggctgg ttccgaaaca
      661 ttgttttgca tattaaaaca taaaaggcgg taaaacctgc atatgggccg gattcacaaa
      721 tattgaaacg agttgaaggc gaatggggct gaggacggct ggacgcttgg acggaggagg
      781 gcttccaagg ggggcgggag ggaggagcga cggaacgtct ccgtcgccct tccccttagg
      841 gtttagcgaa gcttccgcac cgcggtgaag agccccggcc actcgggctc gagggggacg
      901 gccggcttcg cgggagcctg gccggccttg gcgggcgcgg ccgcgccgtc cgtcacgccg
      961 gccgcggcgg gagggacggg cggcgcgagg acgagctcgt cccccacgtt gatcgtgtcg
     1021 aagaagcccg cgctcttgag gctgccctcg cagagctcct cgtcgaccct ggtcacgagg
     1081 agctcgccca cgactgcgct cggggcgtag gcggccccga gcccctcggg cttcacgtcg
     1141 aggctgccgc gcttcaggac gacaaggcgg tctcccgcct tgatcccgtc ggaggagccc
     1201 aggtccgcga gcgccttgtc ctggctgcgc cgcaggaccg agcccttggg ctccagggcg
     1261 ccttcgacca gggaggcgag gcgggaggcc gtgttcttca cccggtcgtt gccgctcctg
     1321 taggcgcggc agacggtggc ggggctcccc gtcctcgcga cgcgcagctc gcctgagagc
     1381 tcgaggtccc gctccgtctc gcgggcgcgg aagacgaggt agtagtcggc ctccgcctcg
     1441 cgggcgcgcc tgaaggcctc cgccgggttc cgggcctcgg cggcgagggg gaggaccgag
     1501 aggcgggagg agcccgcgag caggtccttg aggtagcgga ggaggacggc cgagcccgcg
     1561 gtgtggacgc cctcgccccg gtcgggcagg gtgaagaccg ccaggcggta gggccgcttc
     1621 ggcagggagt actggtccag gctccaggcc cgcgagacgg agtcctcgag gaggctcgag
     1681 tagatctcga tggcgtcgga gaccgccgtg tccgccttgc cgatgcccgc gaggaacttg
     1741 agctccgcga gctgcttccc ggggaggccg cgcttgcgga ggagctccgc gtagaggacg
     1801 cggcccttct tggagtaggg gtagatcctg aggccgcgcc tgtactcgaa gagggcctgg
     1861 tcgaagagac tgcggtcctc gaactcggcg ccgcgggcga agtgccattc ggcgtagggc
     1921 tcgcgggatg ggtcctcgaa gggccccgag tccacgacga ggttctccag ggctatgcgc
     1981 gccacctcgt cgtcgggctt gagggccgcg gcctggcgga ggctgtacag ggcctccgcg
     2041 ccgcgcccgg cgccggcctg ggccaggccg agggtgaacc aggccgcggc gtccttgcgg
     2101 tcgcgggcca cggcctcgcg catcagggcc gcggcctcct cgtaggaggc ggcctcgtac
     2161 atgagggagg cgaggaggcg ccgggcctcc gcgtaggctg ggctcgcgtc cagggcgttc
     2221 cgcgcgtgga agacggcgcg ctcgcggtcg ccctccgcgg cggccaggcg ggcggccgtg
     2281 tactgcaccc gcggctcctc gccgtggaag cgcagggcct tctccacgag gacgcgggcc
     2341 tcctcggccc ggccctggtc tgaggcgacg agggccagcg agaggagggc cctcgcgttc
     2401 tccggcgaga gcctgaggct gtcctccagc ctgagcttcg cgtccgtctt cttgcccgcg
     2461 gccaggtcga ggagggcgag gccgaagcgg gcgtccaggt cgttcggccg cccggccagg
     2521 acggcggaga agagggagcg ggcgcccgcg aggtccccga ggcccaggcg gatgaagccc
     2581 tccaggtcca tgaggacggg ttcgccccgc atgaggggcg cggccttctt ggcgtaggac
     2641 agggcctggt cgtactcctc gagctcgtag tagcactcgg cgaggccgag gagggcctcc
     2701 gcgtaggagg ggttgcgcgc gacggccgcg aggtagtcct ccacggcgcc gtagacgtcc
     2761 tcggcgaaga gcttggcctc cgccgcggcg gcgagggcgc gcgcctccga ggccggctgc
     2821 gcgcccggcg cggccgccgc gaggagggcg aggacggccg cgagggccag ggcgcgggcg
     2881 cggcggcacg acggggagct gggcttgggc tgcacgctag tcctcctcgg gcggccggcg
     2941 ctggaccttg atgagcgtta tgcggttccc gtccatctcc tggacgacga actcgaggcc
     3001 gcccgaggcg atgcgctcgc cgcgggccgg aatcctgccg aagaggtcga agatgtagcc
     3061 gcccagggtc tcgaagtcgt ccgcgggcag ctccaggccg agctcctcgt tgaggtcgtc
     3121 gaggtcggcc cgcgcgtcgc agagccagac gccctcgccc agggaggaga ggtcctc
//
LOCUS       DAOOSJ010000279         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_20441_length_3130_cov_4.995772, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000279 DAOOSJ010000000
VERSION     DAOOSJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20441_length_3130_cov_4.995772"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..559
                     /locus_tag="PLG14_11260"
     CDS             125..559
                     /locus_tag="PLG14_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX49754.1"
                     /translation="MDAIARMGVIFLGRRRLHAENVRPYGLTLKQLFLLQWARAEGAL
                     SPSEAARLLFCDRPTATVIVSNCVKKGWLARRRSTEDGRAVVLELSEEGRALLARVEA
                     EDRLDSGIGDPLDVLGAEEREAFEASLKKVYRRANELFGKGA"
     gene            561..1163
                     /locus_tag="PLG14_11265"
     CDS             561..1163
                     /locus_tag="PLG14_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX49755.1"
                     /translation="MPKELGALEAIAVRRSIRRYSPEPVPAADLQAILKAGLAAPSSG
                     NQRAWRFLVVEREEDRRRLLEESAKALVASLSEERLARNGGAEALLRDTLQAAEGYAT
                     APVIVAVLGDAKGPWPGYLKHDCPIAAATMMIAARSLGYGSVYLSDTVTEAGLRAAFG
                     LSERWVSPCSIALGLPAEEPEAREPADPATYVWRGAPRED"
     gene            complement(1211..2452)
                     /locus_tag="PLG14_11270"
     CDS             complement(1211..2452)
                     /locus_tag="PLG14_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOX49756.1"
                     /translation="MMDTATMRGYAALLLDACLGIGPGARLRVAAEPCHRRLMHAVAE
                     AAYARGARQVRLDYFDPRLSRIRTDSVREEWVEDVSALVRREAETYVEEGWVSLNLVG
                     EEDPKAMEGADPSRVQRALRARSGHVKPFRDAMMSNRLAWCVAPAPTEAWGRSVFLEA
                     GRDPGPDPEAALWAELVPILRLDSPDPAKAAREHGEALQARARALDALALGSLRFQAP
                     GTDLFVRLSPRSRWIGGGSRMPDGRFFLPNHPTEEVFTSPDAGGTEGRAACTRPVDVL
                     GAKVEGAWFEFEGGRVVHFGAARNEAMLGRYLDSDEGARRLGEAALVDATGPIFKSGL
                     VFGNTLIDENAACHIALGASYDEAFEGAAGMDEATKKREGFNDSIVHTDFMIGSERME
                     VTGTARDGREVPILRGGRFAI"
     gene            complement(2482..>3130)
                     /locus_tag="PLG14_11275"
     CDS             complement(2482..>3130)
                     /locus_tag="PLG14_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOX49757.1"
                     /translation="AASTNASAAAATASWSGTSRVEPGPGRLELGLRRGDLVLVDTAP
                     LVYLVEGGPSRGGAGTRGAVLPGGSPPGGSPPGASSGGRAGDAAPDRRARAAAAFFEA
                     AEAGGLRLAASALAWTELLARPLREGDEALSDRFRSFLADSRRFYLAPVDAAIADAAA
                     ALRGRRRLELADAVQIATALVLGAAAVLTNDEAWRRVPDCPRVILLDELAFELES"
BASE COUNT          425 a         1159 c         1136 g          410 t
ORIGIN      
        1 cgtcctggcc tcctccccgg cgcggggctg tgcccggcgt cgcttggccc cgtcgtccgc
       61 gctccctccc gctcaccgat tatgcttgcg tttttccggc gctatagtta gagttctaac
      121 tacaatggac gccatcgccc gcatgggcgt catcttcctc ggaagaaggc gcctccacgc
      181 agagaacgta cggccctacg gcctcacgct caagcagctc ttcctcctcc aatgggccag
      241 ggccgaaggg gccctctcgc cctcggaggc ggctaggctc ctcttctgcg accggcccac
      301 ggcgaccgtc atcgtaagca actgcgtcaa gaagggctgg ctggccaggc gacgctcgac
      361 ggaggacggc cgcgccgtgg tcctcgagct ctcggaggag gggagggccc tcctcgcgcg
      421 ggtcgaggcc gaggaccggc tcgactcggg gatcggcgat cccctcgacg tcctgggcgc
      481 cgaagaaagg gaggccttcg aggcctcgct gaagaaggta taccgaaggg cgaacgagct
      541 cttcggaaaa ggagcatgac atgccgaagg aactcggggc cctggaggcc atcgccgtcc
      601 gtcgctctat ccgccgctac tcgcccgagc ccgtcccggc cgccgacctc caggcgatac
      661 tgaaggccgg cctcgcggcg ccgagctcgg ggaaccagcg cgcctggcgc ttcctcgtcg
      721 tcgagcggga agaggacagg cgccgcctcc tggaggagtc ggcgaaggcc ctcgtggcct
      781 ccctctccga ggagcggctc gcccgaaacg gcggggccga ggccctcctc agggacacgc
      841 tccaggcggc cgagggctac gcgaccgctc ccgtgatcgt cgccgtcctg ggcgacgcga
      901 aggggccctg gcccggctac ctgaagcacg actgccccat agccgccgcc accatgatga
      961 tcgcggcgcg gagcctgggc tacggcagcg tctacctgag cgacacggtg accgaagcgg
     1021 ggctccgcgc cgccttcggc ctctcagagc gctgggtctc gccctgctcg atagccctgg
     1081 gcctgccggc ggaggagccc gaggcgcgcg agcccgccga tcccgcgacc tatgtctggc
     1141 gcggcgcccc gcgcgaggac tgaaaggcgg ccgcggccgc gctcgagcct gcgaggccgc
     1201 cgcccctgcc ctagatcgcg aagcgcccgc ccctcaggat ggggacctct cggccgtccc
     1261 gcgccgtccc cgtcacctcc atcctctcgg agccgatcat gaagtcggtg tgcacgatgg
     1321 agtcgttgaa gccttcgcgc ttcttcgtcg cctcgtccat gcccgccgcg ccctcgaagg
     1381 cctcgtcgta ggaggctccg agcgcgatgt ggcaggccgc gttctcgtcg atgagggtgt
     1441 tgccgaagac caggccgctc ttgaagatcg ggccggtcgc gtcgaccagg gccgcctcgc
     1501 cgagccgcct cgccccctcg tccgagtcga ggtagcggcc gagcatggcc tcgtttctcg
     1561 cggcgccgaa gtggacgacc ctgccgccct cgaactcgaa ccaggcgccc tccaccttcg
     1621 cgcccaggac gtccacgggg cgcgtgcagg cggcccttcc ctcggtgccg cccgcgtcgg
     1681 ggctcgtgaa gacttcctcg gtcggatggt tgggcaggaa gaacctgccg tcgggcatgc
     1741 gcgagccgcc gccgatccag cgcgagcgcg gcgagagccg cacgaagagg tcggtccccg
     1801 gcgcctggaa gcggaggctc ccgagggcga gggcgtcgag cgcccgggcg cgcgcctgca
     1861 gggcctcgcc gtgctctcgg gccgccttcg cgggatcggg cgagtcgagc ctcagtatcg
     1921 ggacgagctc cgcccagagc gccgcctcgg ggtcggggcc cgggtcgcgg cccgcctcca
     1981 ggaagaccga gcggccccag gcctccgtcg gcgccggggc gacgcaccag gccaggcggt
     2041 tggacatcat cgcgtcgcgg aagggcttca cgtgccccga acgggccctg agcgcgcgct
     2101 ggaccctcga agggtcggcg ccctccatcg ccttggggtc ctcctcgccg accaggttga
     2161 gcgaaaccca gccctcctcg acgtaggtct ccgcctcgcg gcggacgagg gcggagacgt
     2221 cctccaccca ctcctcgcgg accgagtccg tccggatgcg cgaaaggcgc ggatcgaagt
     2281 agtccaggcg gacctggcgc gccccgcggg cataggccgc ttcggccacc gcgtgcatga
     2341 ggcggcggtg gcagggctcg gccgccaccc ggagccgcgc tccgggtccg atgccgaggc
     2401 aggcgtcgag gagaagggcg gcgtagccgc gcatcgtggc agtgtccatc atggcagtct
     2461 ctccttgggt cgtccggcgg atcaggattc cagctcgaag gcgagctcgt cgaggaggat
     2521 gacgcggggg cagtccggca ccctccgcca ggcctcgtcg ttcgtgagga cggccgccgc
     2581 gcccaggacc agggccgtcg cgatctggac ggcgtccgca agctcgagcc gcctccggcc
     2641 ccgcaaggcc gcggcggcgt cggctatcgc ggcgtcgacg ggcgcgaggt agaagcgcct
     2701 cgagtcggcc aggaagctcc ggaagcgatc cgacagggcc tcgtcgccct cgcggagggg
     2761 cctcgccagg agctcggtcc aggcgagggc cgaggcggcg aggcggaggc cccccgcctc
     2821 cgccgcctcg aagaaggccg cggccgcgcg ggcccggcgg tccggcgcag cgtcgcccgc
     2881 cctgccgccg gatgacgcgc cgggcgggga gccgcctggc ggcgagccgc cgggcaagac
     2941 ggcgccgcgc gtgccggcgc ctccgcggga cggaccgccc tcgacgaggt agacgagggg
     3001 cgccgtgtcg acgaggacga ggtcgccgcg ccggaggccg agctccaggc gcccggggcc
     3061 gggctccacg cggctagtcc ccgaccagga cgcggttgcg gccgccgcgc ttgcattcgt
     3121 agaggcggcc
//
LOCUS       DAOOSJ010000280         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_20828_length_3094_cov_4.853241, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000280 DAOOSJ010000000
VERSION     DAOOSJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20828_length_3094_cov_4.853241"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1192)
                     /locus_tag="PLG14_11280"
     CDS             complement(236..1192)
                     /locus_tag="PLG14_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOX49758.1"
                     /translation="MRKTRIGLVQNAPKLGRLEENLAEARALVEGAAREGASVVFLPE
                     LSLCGFRPALGQAAAAEPAGGRCDRWLSSTARELGLVLGAGYLEARGGDLFNVYVLAG
                     PDGRVLGRVGKESPSMGETYVFASEPGGHVVDTPLGRVGVGICNDSHMAYLPRKLLEA
                     GAELVVMPHAWPLPDAPSRLVDAADIGRQARNMEELAGIYARSLGVPALLTNLAGRLD
                     MGGERSPGFLGALLPPPESYRFPAFADVRAAGGARIASREGRGEPGFLVAEVELVGHA
                     RKAGGLPAYGRRVYEANADSVILRLYEALGRRSYRRGRRTAE"
     gene            1368..1952
                     /locus_tag="PLG14_11285"
     CDS             1368..1952
                     /locus_tag="PLG14_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49759.1"
                     /translation="MPESGLDPRIKRTRGLIVTAYLEARAKGEARASSVAGVCALAGI
                     SRSVFYRHFEGLADLEARGLGLFLSGLLEESGLEAASGGQGRRLGVGDRVARFFAALE
                     RHRPTILPLLKRSPPLGEALRGVAEEWLGEHRLGRIPDEALRLPRRYAAPLLASSLLA
                     FCAAWLEDPRGEGPEEAASAFLEFATKGLFDFGS"
     gene            complement(2012..2308)
                     /locus_tag="PLG14_11290"
     CDS             complement(2012..2308)
                     /locus_tag="PLG14_11290"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOX49760.1"
                     /translation="MSVLFIANIRMRDEAAYARYLAACDEVFTRFSGRYLAVAEPLRV
                     LEGSYPYSKTVVIEFPDQAALEAWYFSPAYQEILAHRLAGADCDTIELQVRDRA"
     gene            complement(2321..2833)
                     /locus_tag="PLG14_11295"
     CDS             complement(2321..2833)
                     /locus_tag="PLG14_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49761.1"
                     /translation="MDQASIRVTALDYPDESARLLGLLSRKRSVTLATAAGGRVGARS
                     MCLAMRGETCYFQTDAGMAKTGQVRASSAAALCADTLSAEGEARLLEPGPEFEEALEL
                     MRRAHAGKAEGFRPFRDLALIAVRLTRIREWRYDPEGPHEVVVDLAARAAVRRELGPC
                     FGPAEGPRGT"
BASE COUNT          377 a         1128 c         1165 g          424 t
ORIGIN      
        1 cgcccgacgg cgtttccgcc aacgcggccc tcgcgggcct cctcgacgag ctcctggccc
       61 tgctccccgt ccggctcgcc gagcccggcc tcgtcgcgag gatcaggggc cacagcggag
      121 ccctggaggg ctgatcagag gcgtggcgcg gcgcgggctc ggcggccgcc cggcaacccc
      181 agcgcccgcc gcggcgtccg cgccgccaga gggcctcctc gacgagcctt cgcccctact
      241 ccgccgtcct cctcccgcgc cgatagctcc ggcgacccag ggcctcgtag agcctgagga
      301 tcaccgagtc ggcgttcgcc tcgtagacgc gccgtccgta ggcgggcagc ccgccggcct
      361 tccgcgcgtg gccgacgagc tcgacctcgg cgacgaggaa gccgggctcg ccgcgcccct
      421 cgcgggacgc gatccgcgct ccgccggccg cgcggacgtc ggcgaaggcg gggaagcggt
      481 agctctcggg aggcgggagc aaggcgccga ggaagccggg ggagcgctcg ccgcccatgt
      541 cgagcctgcc cgcgaggttc gtgaggagag ccgggacgcc gaggctccgc gcgtagatac
      601 cggcgagctc ctccatgttc cgcgcctgcc gtccgatgtc ggccgcgtcg acgagcctgg
      661 agggcgcgtc cgggaggggc caggcgtggg gcatcacgac gagctccgcg ccggcctcga
      721 gcagcttcct cgggaggtag gccatgtggc tgtcgttgca gatgccgacg ccgacgcggc
      781 caagcggcgt gtcgacgacg tggccgcccg gctccgaggc gaagacgtag gtctctccca
      841 tggaggggct ttccttgccg acccgcccaa ggacgcggcc gtccggcccc gcgaggacgt
      901 agacattgaa gagatccccg ccccgcgcct cgagatagcc cgcgcccagg accaggccga
      961 gctcccgcgc cgtcgaggag agccaccggt cgcagcggcc tccggcgggt tcggcggccg
     1021 ccgcctggcc gagcgccggg cggaagccgc agagcgagag ctccggaagg aatacgacgc
     1081 tcgccccttc ccgggccgcg ccttcgacga gagcccgggc ctcggccagg ttctcctcga
     1141 gcctgccgag cttcggggcg ttctggacca ggcctatccg cgtcttccgc atggctccat
     1201 cctcctttgc cggaagcccg cgaggcggcg ctggctccgg cgcggggcca tgctacgccg
     1261 cggctcccgc ggcggtcgag tccttcttgc tcccgggggg gcgcggcgag gcggccgcga
     1321 gtcccctatg ctacacttga ggccacgact gtcgctcggg agggaccatg ccggagtcgg
     1381 gactcgatcc ccggatcaag cggacgaggg gactcatcgt gacggcctat ctggaagcgc
     1441 gggcgaaggg ggaggcgcgg gcgtcgagcg tcgccggcgt atgcgccctg gcggggatca
     1501 gtcgctcggt cttctaccgg catttcgagg gcttggccga cctggaggcg cggggcctgg
     1561 gccttttcct ctccggactg ctcgaggagt ccggccttga ggcggccagc ggggggcagg
     1621 gacggcggct cggcgtcggg gaccgcgtcg cccgcttctt cgcggccctg gagcggcatc
     1681 ggccgaccat cctgcctctc ctcaagcgga gtccgccgct cggcgaggcc ctgcgagggg
     1741 tggccgagga atggctgggc gagcatcgcc ttgggcgcat tcccgacgag gcgcttcgcc
     1801 tgccccggcg ctacgcggcg cccctcctcg cgtcctccct cctcgcgttc tgcgcggcct
     1861 ggctcgagga tccccgcggc gagggcccgg aagaggccgc gtcggccttc ctcgagttcg
     1921 cgacgaaggg gctcttcgac ttcggatcct gacccgcggc gcggcgcgcc agggggcgga
     1981 ggcggcccgg ggttcctgct tggccgcctt ctcaggcgcg gtcccggacc tggagctcga
     2041 tcgtgtcgca gtccgccccc gcgagcctgt gggccaggat ctcctggtag gcgggcgaga
     2101 agtaccaggc ctccagggcc gcctggtcgg ggaactcgat gacgacggtc ttggagtagg
     2161 ggtaggagcc ctccaggacg cggagcggct cggccacggc gaggtagcgg cccgagaagc
     2221 gggtgaagac ctcgtcgcag gcggcgaggt agcgggcata ggcggcctcg tcccgcatcc
     2281 ggatgttggc gatgaaaagc acgctcatga cgcttcctcc ctaggtcccc cgcggacctt
     2341 cggctggccc gaaacagggg cccagctccc ggcggactgc ggcgcgggcg gcaaggtcca
     2401 cgaccacctc atgggggcct tcgggatcgt agcgccactc ccggatccgc gtcaggcgga
     2461 cggcgatcag ggcgaggtcc cggaaggggc ggaaaccctc ggccttgccg gcgtgggcgc
     2521 ggcgcatcag ctcgagggcc tcctcgaatt ccggtccggg ctccaggagc cgcgcctcgc
     2581 cctcggccga gagcgtgtcc gcgcagagag ccgccgcgga ggaggcgcgg acctggccgg
     2641 tcttggccat gcccgcgtcc gtctggaagt agcaggtctc gccccgcatg gccaggcaca
     2701 tggaccgggc gccgaccctg ccgcccgcgg cggtcgcgag ggtcacggag cgcttgcgcg
     2761 agaggaggcc gaggagcctc gcgctctcgt cagggtagtc cagggctgtg acgcggatcg
     2821 aggcttggtc catggcggag tcctcccgcc ggtcccgccg gcgccctcca tcatgcctcg
     2881 cgcccggctc ggcgtacagc gccggttccg cttttccagg ccgggccggt cggccggccc
     2941 ggcgccgggc gtcgcgaggc gcctgcgcgc caggggcggc ccgcgggctc gcgtggcttc
     3001 tagcccctct accggtccgc gagctccgtc tttcccgcga ggacgtcctt tccgaggatc
     3061 tcgcggagcc gggcagggtc ctcgaagagg gggc
//
LOCUS       DAOOSJ010000281         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_21013_length_3077_cov_4.950364, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000281 DAOOSJ010000000
VERSION     DAOOSJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21013_length_3077_cov_4.950364"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLG14_11300"
     CDS             complement(<1..1160)
                     /locus_tag="PLG14_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOX49762.1"
                     /translation="MQSETGAGPAADEALALLSDIVPFRYLKAAELEALAADAALRSY
                     APGEVLIEQGDSGGDEVFVLLSGSVESVDLSRNPPFRMNVVEAGSYFGERSCLFGSPR
                     LYEMRALAPSACLAIPGGRFLRLLAESRTFAQAFGVKLREGLGIFDAFDRFTAEVLRG
                     ADLGHLEVRRLTELYKALEPALHPLASDEARIDWGALSYAVRRLPGNVTRTFLFLLTD
                     NLPTVYASPSLLFPFVETEARHRFVYEMMPGKDMVLVRNGMSDLMDFVTCLCLLAVEG
                     RKLRYRLNHPDLILALSRCGGPAGDASAAEGDAASAAPAGAPGGDASAAEGEAAGVVG
                     NAVGVEGEAACLPEASGPDEGELLSKLPFSEAERRELEELWPGRAAERVRELV"
     gene            1410..2348
                     /locus_tag="PLG14_11305"
     CDS             1410..2348
                     /locus_tag="PLG14_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX49763.1"
                     /translation="MDRRNGALGSELLLLLTAAIWGFAFVAQRVGMEHVGPFAFNGLR
                     YALGALAVLPLCLASRRRAGAAALSATADGGRGAAPEGARGALSGPRALAACALAGSV
                     LFLGASLQQLGLVTTTAGNAGFITSLYVVLVPLLGLLAGRRASARVWLGAFLALGGLY
                     LISIRSGYRMERGDALELAGAFFWAVHILVISRLAPRMDSFVLAAGQFAVCSALSLAV
                     SALAETTSLAAVRSAALPILYGGILSSGVAFTLQIVAQRKAHPASASIILSMESLFAG
                     IGGILLLGEPFGLRFASGAALMLAGLVLSQLPGRKS"
     gene            2404..>3077
                     /locus_tag="PLG14_11310"
     CDS             2404..>3077
                     /locus_tag="PLG14_11310"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOX49764.1"
                     /translation="MSSYPEEYRERRPDGDAARSPRELEERRQRALDRLTEAFASDLV
                     TMEAYEGRVAAIQAARDREALDGLVAYLPEAAPKRPERQAVRPAGGRTGREPAAARRA
                     SALANRIEPGLSGSESVACVMGDRRLQGDWLQGDKVDSFTLMGSVKYDFRDTALPPGR
                     IKIDAFCLMGDVKVIVPRGLPVKMSAFPFMADARVGREVERRIVPGEPYLDISGFVMM
                     GDIQVSA"
BASE COUNT          407 a         1175 c         1079 g          416 t
ORIGIN      
        1 acgagttccc tcacgcgctc ggccgccctc cccggccaga gctcctcgag ctccctccgc
       61 tccgcctcgg agaagggcaa cttggacagg agctcgccct cgtccgggcc ggaggcctcg
      121 ggaagacagg ccgcttcgcc ctcgactccg acggcgttgc ccacgacgcc tgccgcttcg
      181 ccctcggcgg cggaggcgtc gccgcccgga gcgccggccg gagccgcgga agccgcgtcg
      241 ccctcggcgg cggaggcgtc gccggccggt ccgccgcagc gagagagggc caggatgagg
      301 tcgggatggt tcaggcggta gcgcagcttc cgcccctcga ccgcgaggag gcagaggcag
      361 gtgacgaagt ccatgaggtc ggacatcccg ttccgcacca ggaccatgtc cttgcccggc
      421 atcatctcgt agacgaagcg gtgcctcgcc tccgtctcca cgaaggggaa gaggagggag
      481 gggctcgcgt agacggtcgg caggttgtcc gtgaggagga aaaggaaggt ccgggtgacg
      541 ttcccgggca ggcggcggac ggcgtaggag agggcgcccc agtcgatcct cgcctcgtcg
      601 gaggccaggg gatggagggc gggctccagg gccttgtaga gctccgtgag ccgccgcacc
      661 tccaggtgtc ccaggtcggc gccgcgcagg acctcggcgg tgaagcggtc aaaggcgtcg
      721 aagatgccca ggccctcgcg gagcttcacg ccgaaggcct gggcgaaggt ccgcgactcg
      781 gcgaggaggc ggaggaagcg cccgccgggg atggcgaggc aggcggaggg ggcgagggcg
      841 cgcatctcgt agaggcgggg cgagccgaag aggcaggacc gctcgccgaa gtacgagccc
      901 gcctccacga cgttcatcct gaaaggcgga ttccgcgaga gatcgaccga ctccacggag
      961 cccgaaagga ggacgaagac ctcgtcgccg cccgagtccc cctgctcgat gaggacctcg
     1021 ccgggggcgt agctccgcaa ggcggcgtcg gccgcgaggg cctcgagctc ggccgccttg
     1081 aggtagcgga agggcacgat gtcggagagg agggccaggg cctcgtcggc cgccgggccg
     1141 gctccggttt cgctctgcat gcgatgagga tagacccgcc ccccgcccgg cgcaagcgcg
     1201 gatccgggcc gcgtccgccg ccgcgagacg gcggcgcgac ggacagccgc tcagtgaacg
     1261 gcggcccagc atcgcggcgg cctccacttg cggtcccgcc cccgccgcgg ctaggctgtc
     1321 ccgcggggct ccggccggcg cgggcggctc ccggagccgc cggcccgccc ggggagcgcg
     1381 gcggccttcg gccccgaggg agaaaagcga tggatcgacg gaacggcgcg ctaggctcgg
     1441 aactgctact tctcctcacc gcggcaatct ggggcttcgc cttcgtcgcc caacgcgtcg
     1501 gcatggagca cgtcgggccc ttcgccttca acggcctccg ctacgccctg ggggccctgg
     1561 ccgtcctccc cctctgcctg gcctcgaggc ggcgggcggg agcggccgcc ctatcggcca
     1621 cggcggacgg cggccgcggc gccgcccccg aaggcgcgcg cggcgccctc agcggccccc
     1681 gggccctggc cgcctgcgcc ctcgcgggct ccgtcctctt cctgggcgcg agcctccagc
     1741 agctcggcct cgtgacgacg acggcgggca acgcgggctt catcacgagc ctctacgtcg
     1801 tcctcgtgcc cctcctcggc ctcctcgcgg gccgccgcgc ctcggcccgc gtctggctcg
     1861 gcgccttcct ggccctcggc ggcctctacc tcatctcgat ccgctcaggc taccggatgg
     1921 agcgcggcga cgcgctggag ctcgccggcg ccttcttctg ggccgtccac atcctcgtga
     1981 tctcgcgcct cgcgccccgg atggactcct tcgtcctggc ggccggccaa ttcgccgtct
     2041 gctcggccct gagcctcgcg gtctcggccc tggcggagac gacgagcttg gccgcggtcc
     2101 gctccgccgc cctccccatc ctctacggcg gcatcctctc gagcggagtt gccttcaccc
     2161 tgcagatcgt cgcccagcgc aaggcccatc ccgcgagcgc ctcgatcatc ctctccatgg
     2221 agtccctttt cgcggggatc ggaggcatcc tcctcctcgg cgagcccttc ggcctccgct
     2281 tcgcctcggg cgccgccctc atgctcgcgg gcctcgtcct ctcccagctc cccggacgga
     2341 aaagctgacg aagacgccca ttcggcggta ttattgacgc atccgaacct taggagggga
     2401 cctatgtctt cctatcccga agagtaccgc gagcgccgcc cggacgggga cgccgcgcgc
     2461 tcgccccgcg agctcgagga gcgccgccag cgggccctcg accgcctcac cgaagccttc
     2521 gcctccgacc tcgtcacgat ggaggcctac gagggccggg tcgccgccat ccaggccgcc
     2581 cgcgaccgcg aggccctcga cggcctcgtc gcctacctcc ccgaggcggc gcccaagcgg
     2641 cccgagcgac aggccgtccg cccggcagga ggccgcacgg ggcgcgagcc ggccgcggcc
     2701 cgccgggcct cggccctcgc gaaccgcatc gagcccggcc tctcgggctc ggagagcgtc
     2761 gcctgcgtga tgggcgaccg ccgcctccag ggcgactggc tccaggggga caaggtcgac
     2821 tccttcaccc tcatgggcag cgtgaagtac gacttccgcg acaccgccct gccgcccggc
     2881 aggatcaaga tcgacgcctt ctgcctcatg ggcgacgtga aggtcatcgt gccccgcggc
     2941 cttcccgtca agatgagcgc cttccccttc atggccgacg cccgcgtcgg gcgcgaggtc
     3001 gagcgccgca tcgtccccgg cgagccctac ctggacatct cgggcttcgt gatgatgggc
     3061 gacatccagg tctcggc
//
LOCUS       DAOOSJ010000282         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_21064_length_3073_cov_5.731279, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000282 DAOOSJ010000000
VERSION     DAOOSJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21064_length_3073_cov_5.731279"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLG14_11315"
     CDS             <1..541
                     /locus_tag="PLG14_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49765.1"
                     /translation="LTLGSVLAALKEGLLPGLQSGGLGLGIYAALRVGVPFYLSSGGF
                     LGALAAGLLLWVGVVLLLALQWYLPLRARLGGGFAKNLRKSLVLFFDNGLFSLFLFLY
                     NAFGLALSFFLAFLVPGPAGVALALDDALRLRLYKYDWLEANPGAKRGDVPWESLLSE
                     DKELVGKRSLKGMLFPWKE"
     gene            618..1601
                     /locus_tag="PLG14_11320"
     CDS             618..1601
                     /locus_tag="PLG14_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOX49766.1"
                     /translation="MSITTSQQIAKWYDVYKSIDVTLTKEILKTTGLDPRHVFMKCVG
                     EQWPCVIYSTSFSGAKIIVSAKPILLEKIKRANNLVSLRFSFRFEGKADPLSFFVTGR
                     ATGFAPYQAGGELQFMTLEYTQRPPDDLIEIMGRLLEANINSARRREERILLNPDSMR
                     RLCLMSKDTILTVQGVPRKCIVRDLSFSGAKLIIVGLAKFLVGKECLLRLEMDEPRET
                     LDLKGSIVRYEDVEGRKDLTAVAVRFEDQTVPMTYKIHINDFLGQVRQVRPENAPGAE
                     GASTAQPAAARPAPAQPAPAQAASAQPAESPAPAGPAPAKAGGGVPHGQSR"
     gene            1585..2724
                     /locus_tag="PLG14_11325"
     CDS             1585..2724
                     /locus_tag="PLG14_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX49767.1"
                     /translation="MANPARDEEAGEAALRRLVFIDLPPALAREIGSFRPDPAIPLPV
                     EPGVEPERFDPRDLSWEAIVAGMLRLLAWRPEGEHADYYRSFVKAVKPRILAELSEAG
                     IAKARNKEWEVAEEIFRALAGLYPDAPEPLIDLAVLYEDRADAYAAVDKEALAEEMDS
                     RAFEAYKRLLSMEPPFPEAYFNAGFFFLRRRNYERAMSLFETYQKIGEDEERKARAAD
                     IVKTLSSQGLLDQLFKEAYDFIRLGKEEEGLEKARAFLEKNPRVWNGWFLLGWGARRL
                     GRWEDGRLAFEKALELGAEGTDVLNELSICLLELGRLEEAKRALEKALRAEPENVKII
                     TNLGAVALKRGRRDEAAGFFRTALELEPEDAAARRWLAGIEGREA"
     gene            2793..>3073
                     /locus_tag="PLG14_11330"
     CDS             2793..>3073
                     /locus_tag="PLG14_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49768.1"
                     /translation="MASRLEGLKAARLLAAAALLKNRVRPPGVADLSRLVSRARAVLD
                     SEDERLRPRDSRGLPGGLLDLPDLPTVLVPDLHARPGFLAAVLAWRPPLG"
BASE COUNT          456 a         1203 c          959 g          455 t
ORIGIN      
        1 actcaccctc ggatcggtcc tggccgccct caaggagggg cttctccccg gcctccagtc
       61 cggcggcctg ggcctgggca tctacgccgc cctgcgggtc ggcgtgccct tctacctctc
      121 aagcggcggc ttcctgggcg ccctggccgc cggcctcctc ctctgggtgg gcgtcgtcct
      181 cctcctcgcc ctccaatggt atctcccgct gcgggccagg ctcggcggcg gattcgcgaa
      241 gaacctgcgg aagagcctcg tcctcttctt cgacaacgga ctcttctccc tcttcctctt
      301 cctctacaac gccttcggcc tcgccctctc cttcttcctc gccttcctcg tccccggccc
      361 cgccggcgtc gccctcgccc tggacgacgc cctgcgcctg cgcctctaca agtacgactg
      421 gctggaggcc aacccgggag cgaagcgcgg ggacgtcccc tgggagtccc tcctctcgga
      481 ggacaaggag ctcgtcggca agcgcagcct caagggcatg ctctttccct ggaaagaata
      541 agccgggaag gcgcggcccg ggcggccctc gtcgttgacc aaggcccctc cgatgcccta
      601 ttattccgcc aaggcctatg tccatcacca ccagccagca gatcgccaaa tggtatgacg
      661 tctacaagag catcgacgtc acgctgacga aggaaatcct caagacgacg ggcctcgacc
      721 ccaggcacgt cttcatgaag tgcgtcgggg agcagtggcc ctgcgtcatc tactcgacct
      781 ccttctccgg ggccaagatc atcgtcagcg ccaagcccat cctcctcgag aagatcaagc
      841 gcgccaacaa cctcgtctcc ctgcgcttca gcttccgctt cgagggcaag gccgatcccc
      901 tgagcttctt cgtcacgggc cgggcgacgg gcttcgcccc ctaccaggcg ggcggcgagc
      961 tccagttcat gaccctcgag tacacgcagc gccccccgga cgacctcatc gagatcatgg
     1021 gccgcctcct ggaggccaac atcaactcgg cccggcgccg cgaggagcgc atcctcctca
     1081 atccggactc gatgcgcagg ctctgcctca tgtccaagga cacgatcctc accgtccagg
     1141 gcgtgccgcg gaagtgcatc gtccgagacc tgtccttctc cggcgcgaag ctcatcatcg
     1201 tcggcctcgc caagttcctg gtcggcaagg agtgcctcct ccgcctggag atggacgagc
     1261 cgcgggagac cctggatctc aagggctcga tagtccgcta cgaggacgtc gagggccgga
     1321 aggacctcac cgccgtcgcc gtgcgcttcg aggaccagac cgtcccgatg acctacaaga
     1381 tccacatcaa cgacttcctg ggccaggtca ggcaggtcag gcccgagaac gcccccggcg
     1441 ccgagggcgc ctctacggcc cagcccgcgg ccgcgaggcc ggcccccgct cagcctgccc
     1501 ccgcccaggc ggcctcagca caaccagccg aatcccccgc cccggcggga ccagctcccg
     1561 ccaaggcggg tggaggagta ccccatggcc aatcccgcta gggacgagga ggccggcgag
     1621 gccgccctcc gccgtctcgt cttcatcgac cttccccccg ccctggcgcg ggagataggc
     1681 tccttcaggc ccgaccccgc catccccctc cccgtggagc ccggcgtcga gcccgagcgc
     1741 ttcgacccgc gggacctgag ctgggaggcc atcgtcgccg gcatgctcag gctcctggcc
     1801 tggcggcccg agggcgagca cgccgactac taccgctcct tcgtgaaggc ggtgaagccg
     1861 cggatcctcg ccgagctctc ggaggcgggc atagccaagg cccgcaacaa ggagtgggag
     1921 gtggcggagg agatcttccg cgccctcgcg ggcctctatc ccgacgcgcc cgagcccctc
     1981 atcgacctgg ccgtcctcta cgaggacagg gcggacgcct acgcggccgt cgacaaggag
     2041 gccctggccg aggagatgga ctcccgcgcc ttcgaggcct acaagcgcct cctctccatg
     2101 gagccgccct ttcccgaggc ctacttcaac gccggcttct tcttcctccg ccgcaggaac
     2161 tacgagcggg ccatgagcct cttcgagacc taccagaaga tcggcgagga cgaggagcgg
     2221 aaggcccgcg ccgcggacat cgtgaagacc ctctcctccc agggcctcct ggaccagctc
     2281 ttcaaggaag cctacgactt catccgcctg ggcaaggagg aggagggcct ggagaaggcc
     2341 cgcgccttcc tggagaagaa cccccgcgtc tggaacggct ggttcctcct cggctggggc
     2401 gcccgccgcc tgggccgctg ggaggacgga cgcctcgcct tcgagaaggc cctggagctc
     2461 ggcgccgagg gcacggacgt cctcaacgag ctctccatct gcctcctgga gctcggccgc
     2521 ctggaggagg ccaagcgggc gctggaaaag gccctccgcg ccgagcccga gaacgtgaag
     2581 atcatcacga acctgggcgc tgtcgccctg aagcggggcc gccgcgacga ggcggcgggc
     2641 ttcttcagga cggccctgga gctcgagccc gaggacgcgg cggccaggcg ctggctggcc
     2701 gggatcgaag gccgggaggc ctaggtcttg tccggccgcg gccgcccggc ggcccgcgcc
     2761 gcggggcgcg agccgagcgc gggggcctga gcgtggcctc ccggctcgag gggctcaagg
     2821 ccgcccgcct cctcgcggcg gccgccctcc tcaagaaccg cgtccgcccg cccggggtcg
     2881 ccgacctctc tcgcctcgtc tcccgcgccc gcgccgtcct cgactccgag gacgagcggc
     2941 tccggccccg cgactcccgg ggcctgcccg gcggcctcct cgacctcccc gacctgccca
     3001 ccgtcctcgt gcccgacctc cacgcccgcc ccggcttcct cgcggccgtc ctggcctggc
     3061 gcccgcccct ggg
//
LOCUS       DAOOSJ010000283         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_21483_length_3034_cov_5.798590, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000283 DAOOSJ010000000
VERSION     DAOOSJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21483_length_3034_cov_5.798590"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1390)
                     /locus_tag="PLG14_11335"
     CDS             complement(122..1390)
                     /locus_tag="PLG14_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49769.1"
                     /translation="MKQLRSLYRDYAEAFRHFSPNAKRFLAGSFLLGVGANQISLLFS
                     LYLKRLDYTEAAIGGVLATRALGSTLVALPASILAARFDPRRLLPVAAVLTAAAYAAQ
                     SLAVSGSAIGAAVFLAGAISTVFQVSSGPFFMRNSGEKERMHLFSLNGALSMGTGLLG
                     SLLGGGLKDGLFALGLSEITAYRIALLVGAAFVFAAFLPFARIEASSAPLPERRTAKP
                     RRFEGIDPLLWAKLILPGFLVGMGAGLTIPYLNLYFKNVFGLGDAAIGAAVAAGQVAT
                     FVGMAAGPAIAKRLGKPRAVFWTQILSVPLILVLGWVRALPLALLAYLARQVLMNMST
                     PIQDNFALELVPPERQSLINALKMLSWTGSWTIAARISGDLIYESGFSTSFLLTACLY
                     AASTLCYRIFFIRGAGPGKGSDGRPAGRTA"
     gene            1585..2184
                     /locus_tag="PLG14_11340"
     CDS             1585..2184
                     /locus_tag="PLG14_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49770.1"
                     /translation="MQEAGTGRGEAGDRLLKAARSLVARKGYKGATTKEIAEAAGVNE
                     VTLFRNFGTKERLLYEAIAGSFPEVEEAPLARLLDRPARTRREAVELVADFAAFFAER
                     ALGHNRELILIAMRESGDRPELRELFASRSVLVFRLLERRLKELEAEGLIREGSGGPA
                     ARLLVSALLGNFALSEGAQGGLGLDPREAAELVLSGSLA"
     gene            complement(2181..2870)
                     /gene="trmB"
                     /locus_tag="PLG14_11345"
     CDS             complement(2181..2870)
                     /gene="trmB"
                     /locus_tag="PLG14_11345"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOX49771.1"
                     /translation="MEDQDEMSDLSIRTYVLRAGRMTDGQRKALAELSPRYCLPFDPS
                     RPLDLAAAFGNCRPVVAEIGFGMGKATWRIAEARPETNYLGMEVHTPGVGKLVAELHE
                     RGIGNVRILHHDAVEALEKVIPPASLAGFHIFYPDPWPKKRHHKRRLLRPGIVELLVS
                     RLAPGGYLYFVTDIEDYGQSTLELLSGTAGLKNRYEGFAPRQEWRPETKFEARASYAG
                     RGVFEVLFERI"
     gene            complement(2849..>3034)
                     /locus_tag="PLG14_11350"
     CDS             complement(2849..>3034)
                     /locus_tag="PLG14_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49772.1"
                     /translation="TLLLAYDGAGALVKAAAARHGRNDLAAVLGAAEAGKAAALASYG
                     YDPARRIGAYSWKTRTR"
BASE COUNT          496 a          944 c         1141 g          453 t
ORIGIN      
        1 aactcggcgc ctggggcgag ctccgagggc gcgaggacgg cgttcgccag ggcgtaggcc
       61 gccgaggccg ccagggtcgc gaagacgacg atcgccgcga tcttcgtcca gtcgggcttt
      121 ttcacgctgt cctccccgcg ggccttccgt cactgccctt gccgggcccc gctccccgta
      181 tgaagaagat cctatagcag agcgtcgagg ccgcatagag acaggccgtg agcaggaagg
      241 aggtcgagaa gccgctttcg tagatgaggt cgccggagat cctcgcggcg atggtccagc
      301 tgcccgtcca ggagagcatc ttgagggcgt tgatgaggct ctgccgttcg ggcgggacca
      361 gctccagggc gaagttgtcc tggatcggcg tagacatgtt catgaggact tgcctggcca
      421 ggtaggccag gagggcgagg gggagggcgc gcacccagcc caggacgagg atgagcggca
      481 ccgagaggat ctgcgtccag aacacggcgc ggggctttcc gagccgcttg gctatggccg
      541 gtcccgccgc catgccgacg aaggtggcca cctggcccgc ggcgaccgcc gcgccgatgg
      601 ccgcgtcccc caggccgaag acgttcttga agtagagatt taggtagggg atggtgaggc
      661 cggctcccat gcccacgagg aagccgggca ggatgagctt ggcccagagg aggggatcga
      721 tgccctcgaa gcgccggggc ttcgccgtcc tccgttcggg aaggggagcg gaggaagcct
      781 cgatgcgggc gaaggggagg aaggcggcga agacgaaggc cgcgccgacc aagagggcga
      841 tccggtaggc cgttatctcc gaaaggccca gggcgaagag gccgtccttg agccccccgc
      901 ccaggaggga gcccaagagg cccgtgccca tggagagggc gccgttcagg gagaagaggt
      961 gcatcctctc cttctcgccc gagttgcgca tgaagaaggg cccggagctc acctggaaga
     1021 ccgtggagat ggcgccggcc aggaagacgg ccgcgccaat cgcggagcct gagaccgcga
     1081 gggactgcgc ggcgtaggca gcggccgtga ggacggcggc gacgggcagg agacgccgcg
     1141 gatcgaagcg ggccgcgagg atagaggcgg gcagggcgac gagggtcgag cccagggccc
     1201 gggtcgcaag gacgccgcct atggccgctt cggtgtagtc gagccgtttc aggtagaggc
     1261 tgaagaggag gctgatctgg ttggccccga cacccaggag gaaggagccc gccaggaagc
     1321 gtttcgcgtt cgggctgaag tggcggaagg cctccgcata gtcgcggtac agactgcgga
     1381 gttgtttcac aaagtggagc ttagccgcgc cagagctcca aggtcaaccg cggctggcgc
     1441 aagtccgcgc tggcggaaag cggattccat tcctaggtaa ctacttattg tgagcaagca
     1501 aatggttgca ttatggtttt ttcctggcgc gaggattgca agcctctgct tgcatctctg
     1561 tgagcccgca actacacttg aggtatgcaa gaagcaggca ctggtcgggg agaggcgggc
     1621 gacaggctcc tcaaggcggc gcggagcctc gtcgcgagga agggctacaa gggagcgacg
     1681 accaaggaga tcgcggaggc cgccggcgtc aacgaggtta ccctcttcag gaacttcggc
     1741 acgaaggaga ggctcctcta cgaggcgata gcagggagct tcccggaggt cgaggaggcc
     1801 cccctcgcgc ggctcctcga ccggccggcc aggacgcggc gggaggcggt cgagctggtc
     1861 gcggacttcg ctgccttctt cgccgagcgg gccctcggcc acaaccgcga gctcatcctc
     1921 atcgcgatgc gcgagtcggg ggacaggccc gagctgcgcg agctcttcgc ctcccgctcc
     1981 gtcctcgtct tccgcctcct cgagcggcgc ctcaaggaac tcgaggccga gggcctcatc
     2041 cgcgagggct ctggcggacc ggcggcgcgg cttctcgtct ccgccctcct cgggaacttc
     2101 gccctctccg agggcgccca gggcgggctc ggcctcgacc cgcgggaggc cgccgagctc
     2161 gtcctctcgg gctcgctcgc ctagatcctc tcgaagagga cctcgaagac gccgcgcccg
     2221 gcgtagctcg cccgcgcctc gaacttcgtc tcgggccgcc attcctggcg gggggcgaag
     2281 ccctcgtagc ggttcttgag gccggcggtg ccggagagga gctcgagggt ggactggccg
     2341 tagtcctcga tgtcggtgac gaagtacagg tagcctccgg gggcgagccg ggagacgagg
     2401 agctcgacga tgccgggcct gaggaggcgg cgcttgtggt ggcgcttctt gggccagggg
     2461 tcggggtaga agatgtggaa gcccgcgagg gaggccggcg ggatgacctt ctccagggcc
     2521 tcgaccgcgt cgtggtggag gatgcggacg tttcctatgc cccgctcatg gagctcggcg
     2581 acgagcttgc cgacgccggg ggtgtggacc tccatcccga ggtagttggt ctccggcctc
     2641 gcctcggcga tgcgccaggt agccttgccc atcccgaagc cgatctccgc gacgacgggg
     2701 cggcagttgc cgaaggcggc cgccaggtcc aggggccgcg aggggtcgaa ggggaggcag
     2761 tagcgggggc tgagctcggc cagggccttc ctctggccgt ccgtcatgcg gccggctcgg
     2821 aggacatagg tcctgatgct caagtcgctc atctcgtcct ggtcttccat gaatacgctc
     2881 cgatgcgccg cgcggggtcg tagccgtagg aggcgagggc cgcggccttt cccgcctccg
     2941 ccgccccgag gacggccgcc aagtcgttcc tgccgtggcg ggcggccgcg gccttcacaa
     3001 gggcccccgc gccgtcgtag gcgaggagga gggt
//
LOCUS       DAOOSJ010000284         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_21786_length_3004_cov_6.575449, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000284 DAOOSJ010000000
VERSION     DAOOSJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21786_length_3004_cov_6.575449"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PLG14_11355"
     CDS             complement(<1..2033)
                     /locus_tag="PLG14_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX49773.1"
                     /translation="MIKHRIAGAAAALALAAGALLALSACQTGRDGNAAAQAYTPGRY
                     AAKAMGLGGPVEVTVTVDGSKITDVAIVADKETEAIGGAAVKALREQIIATQGAKVDV
                     ISGATRTSEAVKEALAEALAAAKGQERAKALVDGKYVTRAMGHEGYVNVATVFRGGAI
                     ESCKVISHEETMGIGNYAAARIPGRIVKAQSLAVDSVSGATVTSSAVKAAVAQAVKDA
                     GGDLAAFQKAPPAAPVAKESVARKVDVVIAGAGTAGLMAGARLVEQGKTVLIFEKQDI
                     PGGAMPMTYGGIQSSDTAVGAAYGQGREKKDAAWNKDLLAGIYKNMIRPQYDRFNKAQ
                     PYKTAALDASGPMVDWMREIGVGFAPMGKYEGALQLGLQPYMAPGCYQGGAGYAAMLL
                     ADRIVAKGGTIVYATPVTSLKVEGGRVVGLKAEGKDGKSWDVEAGAVMLATGGFASNR
                     EMIAQYYPAYKDFAFNGVAGDTGEGIVMGQAAGAAIECMDRALGAFPSAYGSNFELAF
                     INMVVPGILVAGEGHVVADASHVGIGNARLDPANKDRFYYVFDDEGVEAMKKSMNYGF
                     SYGSVFERGEAVRYASVEEAAAKLGLPKLAAVIQENNANALAKKGKASYVETRDGIWL
                     IRIVPCFYLTTGGLAIDTEARVLDKAGKAIPGLYAAGDVVGSIEEKDGLVYGYG"
     gene            2463..>3004
                     /locus_tag="PLG14_11360"
     CDS             2463..>3004
                     /locus_tag="PLG14_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOX49774.1"
                     /translation="MAGQTDRILVLASAFGDLDEARVEAEVESLLQGGMRPREIQKIL
                     ELGMRIVAENYRIGEYFLADRLFAGRIYRQIMELPRMSSALPGAAPLGTVLVGTLPGE
                     LHDEGNDALAAALRSLGFRAIDLGLGAEADELEAALVELEPCAAVLSCWRAGAAAGMA
                     PLVRRLRGAFPSMGGLALRGG"
BASE COUNT          433 a          989 c         1041 g          541 t
ORIGIN      
        1 ccgtagccgt agaccaggcc gtccttctcc tcgatggagc ccacgacgtc gccggcggcg
       61 tagaggccgg ggatggcctt gccggccttg tccaggacgc gggcctcggt gtcgatcgcc
      121 aggccgcccg tggtcaggta gaagcagggc acgatgcgga tgagccagat cccgtcgcgg
      181 gtctccacgt acgaggcctt gcccttcttc gcgagggcgt tggcgttgtt ctcctggatc
      241 accgcggcca gcttgggcag tccgagcttg gcggcggcct cctcgaccga ggcgtacctg
      301 acggcctcgc cgcgctcgaa gaccgagccg taggagaatc cgtagttcat ggatttcttc
      361 atggcctcca cgccctcgtc gtcgaagacg tagtagaagc ggtccttgtt cgcgggatcg
      421 aggcgggcgt tgccgatgcc gacatggctc gcgtcggcca ccacatgccc ctcgccggcc
      481 accaggatgc cgggcacgac catgttgatg aaggccagct cgaagttcga gccgtaggcc
      541 gaggggaagg cgcccagagc ccggtccatg cactcgatcg cggcgccggc cgcctggccc
      601 atgacgatgc cctcgcccgt gtccccggcg acgccgttga aggcgaagtc cttgtaggcg
      661 gggtagtact gggcgatcat ctcgcggttg gacgcgaagc cgccggtcgc gagcatgacc
      721 gcgccggcct cgacgtccca ggacttgccg tccttgccct cggccttgag gccgacgacg
      781 cggccgccct cgaccttgag ggaggtgacg ggggtggcgt agacgatggt cccgcccttg
      841 gcgacgatgc ggtcggcgag gagcatggcc gcgtagcccg ccccgccctg gtagcagccg
      901 ggggccatgt agggctggag gccgagctgg agggcgccct cgtacttgcc catgggggcg
      961 aagccgacgc cgatctcgcg catccagtcc accatggggc ctgaggcgtc gagggcggcg
     1021 gtcttgtagg gctgggcctt gttgaagcgg tcgtactggg gccggatcat gttcttgtag
     1081 atgccggcga gcaggtcctt gttccaggcg gcgtccttct tctcccggcc ctgcccgtag
     1141 gcggcgccga ccgccgtgtc ggagctctgg atgccgccgt aggtcatggg catcgcgccg
     1201 ccggggatgt cctgcttctc gaagatcagg acggtcttgc cctgctcgac caggcgggcg
     1261 ccggccatca ggcccgcggt gcccgcgccg gcgatgacga cgtcgacctt ccgggcgacg
     1321 gactctttcg cgacgggcgc ggcgggcggg gctttctgga aggccgccag gtcgccgccg
     1381 gcgtccttga cggcctgggc cacggcggcc ttgacggcgc tcgaggtgac cgtcgcgccg
     1441 gagaccgagt cgacggccag gctctgggcc ttcacgatgc gccccgggat cctggcggcg
     1501 gcgtagttgc cgatgcccat ggtctcctcg tgggagatca ccttgcagct ctcgatggcg
     1561 ccgccgcgga agacggtggc cacgttgacg tagccctcgt ggcccatggc cctggtgacg
     1621 tatttgccgt ccaccaaggc cttggcccgc tcctggccct tggccgcggc gagggcctcg
     1681 gcgagggcct ccttgacggc ctcgctggtc cttgtggcgc cgcttatcac gtctaccttg
     1741 gcgccctggg tggcgatgat ctgctcgcgc agggccttga cggcggcgcc gccgatggcc
     1801 tcggtctcct tgtcggcgac gatggccacg tcggtgatct tcgatccgtc gacggtgacc
     1861 gtgacctcga ccgggccgcc taggcccatg gctttcgcgg cgtatcggcc gggcgtgtag
     1921 gcctgcgccg cggcgttccc gtccctgccg gtctggcagg ccgagagggc gagaagcgcc
     1981 ccggccgcca gcgcgagggc cgcggcggcc ccggcgatgc gatgctttat catgaagaac
     2041 ctccttgggt ggctatgctg gatgcgcgag cctttggcgt tccgcccctg gcgcggagcc
     2101 ttccgaccct atccccgagg cctacgacca ggccttcggg gtggacgagc ggcatgacct
     2161 tcgatgcaag gcgttgtcca tgcttgcctg tccaggaact caaaagatgg cctagcatag
     2221 cctataacga gctacttgta aatacatgta tgaaaaccgg attttgctca tctatatatg
     2281 cgaattatat ataacaaaca aatataagta cgcggcttcc ctggtttcgc cgaaatgcca
     2341 gacttattga agccttcttg tatttacata tagacaatac ttctgctccg ggaatatcgt
     2401 ttccctgatc ccggtgctat actcgttcgc ggcgtgagcg acaaggaaga aggacgagca
     2461 gcgtggcagg acaaacagac aggattctcg ttctggcgag cgccttcggc gatctcgacg
     2521 aagcccgcgt ggaggcggag gtcgagagcc tccttcaggg gggcatgcgc ccgcgcgaga
     2581 tccagaagat cctcgagctg ggcatgcgga tcgtcgcgga aaactaccgc atcggcgagt
     2641 atttcctggc cgatcggctg ttcgcaggca ggatctaccg ccagatcatg gagctgccgc
     2701 ggatgagttc ggccttgccc ggggcggcgc ccctcggaac cgtgctcgtc gggaccctgc
     2761 cgggagaact ccacgacgag ggcaacgacg ccctcgcggc tgccctgcgc tccctgggct
     2821 tccgcgccat cgacctgggc ctgggcgcgg aggccgatga gctcgaggcg gccctcgtcg
     2881 agcttgagcc ctgcgccgcg gtgctcagtt gctggcgcgc cggcgcggcg gccggcatgg
     2941 cccccctcgt ccgaaggctt cgaggcgcct tccccagcat gggagggctc gcgctccgcg
     3001 gcgg
//
LOCUS       DAOOSJ010000285         2998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_21843_length_2998_cov_5.306490, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000285 DAOOSJ010000000
VERSION     DAOOSJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21843_length_2998_cov_5.306490"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..798)
                     /gene="tpiA"
                     /locus_tag="PLG14_11365"
     CDS             complement(49..798)
                     /gene="tpiA"
                     /locus_tag="PLG14_11365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOX49775.1"
                     /translation="MRNYFIAGNWKMHKTVGESVALAKELAAQLSGSNEKVMIAPAFT
                     ALAAVSEALKGSKILLGAQNMGPEEQGAHTGEVSVLMLKDLGVKVVILGHSERRHSYL
                     ESDELVNKKVRLALQHGLEVILCVGETLEEREGGRLETVCRTQVEKGLAGVSAADLAR
                     VTVAYEPVWAIGTGKTATPEDADAVHAYVRKVLAGLYGEAAAKAVCIQYGGSVKADNA
                     AQLMAKANIDGALVGGASLKPETFVPIVKFR"
     gene            complement(1100..1693)
                     /locus_tag="PLG14_11370"
     CDS             complement(1100..1693)
                     /locus_tag="PLG14_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX49776.1"
                     /translation="MAKRQEATKRKAKAEESKNRIYETALAVIGEKGFEATTILDITA
                     RAGVAIGTFYHYFHSKSGILEENFRRADEAFASFAATPGLKEGPAGERILRYLDRYAL
                     LIEETGIDLIKQLYTSKNKLFIRRGRAMQEGLIALLAEAQARGELKPEPGPEGICDYL
                     FIAARGVVFHWCLLEGEKSIRELMREHFAPLVPIFLA"
     gene            1864..>2998
                     /locus_tag="PLG14_11375"
     CDS             1864..>2998
                     /locus_tag="PLG14_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX49777.1"
                     /translation="MSKRTARPLAVLALLAAASLALVAAGPYAGAAGKPSYLNGVYSG
                     SAPSHGGVLTVSVTVKKGKIASVAVKATDSAGISDAAIDKIPAAIVKAQSAEVDAVAG
                     ASETSEAIKAAVAKALAGATLYPVASTMKTDVVVLGGGNAGLAAAVEAANAGAKVVLV
                     EKMAFLGGNSIRAGGAYNAVDPERQEKQGIVDSIDKHYEQTLSGGDGKGDPALVRTLV
                     EGSPKGLKWLEGMGMKFNDKVFTVLGALWPRSHSPVEPVGTGFIKTLKAAAEGKGVKF
                     LLETKADQIIREDGRVKGVVVVDAAGKRIRINASRGVVLATGGFAANKEMRQAYDPRI
                     TPNLGTTNHPGATGDGVVMAEKIGAALVGMEYIQMLPLGDPKTG"
BASE COUNT          465 a         1026 c         1041 g          466 t
ORIGIN      
        1 gggctcgccg cggccggggc cgactggtcc gcgaacgacc gcgggggcct agcggaactt
       61 gacgatgggg acgaaggtct cgggcttgag gctggcgccg cccacgaggg cgccgtcgat
      121 gttcgccttg gccatgagct gggcggcgtt gtcggccttc accgagccgc cgtactggat
      181 gcagacggcc ttggcggcgg cctcgccgta gaggcccgcg aggaccttgc ggacgtaggc
      241 gtggacggcg tcggcgtcct cgggggtggc ggtcttcccg gtgccgatgg cccagacggg
      301 ctcgtaggcg accgtcacgc gggcgaggtc ggcggctgag acgcccgcga ggcccttctc
      361 cacctgggtc ctgcagacgg tttcgaggcg gccgccctcg cgctcctcca gggtctcgcc
      421 cacgcagagg atgacctcca ggccgtgctg gagggccagg cggaccttct tgttgacgag
      481 ctcgtcggac tcgaggtagc tgtggcggcg ctcggagtgc ccgaggatca cgaccttgac
      541 gcccaggtcc ttgagcatga ggacggagac ctcgcccgtg tgggcgccct gctcctccgg
      601 gcccatgttc tgggcgccga ggaggatctt cgagcccttg agggcctcgg atacggcggc
      661 gagggcggtg aaggcggggg cgatcatgac cttctcgttc gagcccgaga gctgggccgc
      721 gagctccttc gcgagggcga cggactcgcc gaccgtcttg tgcatcttcc agttgccggc
      781 gatgaaatag ttgcgcatag gccgtccaga tgggggggat gggcgccgcg cctggccccg
      841 gggatgacgg gcatcgaaag gcagccgggg gcgtcgtccg gcgcgtaaag cccctcaggc
      901 gggggccgcg ccgcttctca tagtaacaag ccgcctcttc ttggtaaagc cgtcaggcgc
      961 ggtcggcgct cgcggcgccg ccgcgaggac ggcgccgccg gcgcggccgg catgcgagtc
     1021 gggcgcgtca tgcgcgacgc cggagcctcg ggcggcgccg cctgcgcgga gggcggaggc
     1081 cgcgcggccc ggtccgatcc taggcgagga agatcgggac caggggggcg aagtgctcgc
     1141 gcatgagctc gcgtatggac ttctcgccct cgaggaggca ccaatggaag acgacgccgc
     1201 gggcggcgat gaagaggtag tcgcagatcc cctcggggcc cggctcgggc ttgagctcgc
     1261 ccctcgcctg ggcctcggcc aggagggcga tgaggccctc ctgcatcgcc cggccccggc
     1321 ggatgaagag cttgttcttg ctggtgtaga gctgcttgat gaggtcgatg ccggtctcct
     1381 cgatgaggag ggcgtagcgg tccaggtagc gcaggatgcg ttctcccgcc ggcccttcct
     1441 tgagccccgg ggtggccgcg aaggaggcga aagcctcgtc cgccctgcgg aagttctcct
     1501 cgaggatgcc gctcttggaa tggaaatagt ggtagaaggt gccgatcgcg acccccgccc
     1561 tggcggtgat gtcgaggatc gtcgtcgcct cgaagccctt ctcgccgatg acggcgaggg
     1621 ccgtctcgta gatcctgttc ttgctttcct cggccttagc cttgcgtttc gtcgcttcct
     1681 gccgtttagc catggaaacc gccttcgggc ccaaggtacc agaatgaagg gattccggca
     1741 atgacgcatt ttatgaaatt cgttcggcga atgcatttca tgtcttgact agaactcatt
     1801 ctagatatat tacgataaat aaagcgtgtt tacgcccacc acagtcgttt cctggaggaa
     1861 atcatgtcca agcgaacggc caggcccctt gcggtcctgg cgctcctcgc cgcggcctcg
     1921 ctcgccctcg tcgcggcggg cccctacgcc ggcgccgccg gcaagccgag ctacctgaac
     1981 ggcgtctact ccggctccgc cccgagccac ggaggcgtcc tcacggtctc cgtcaccgtc
     2041 aagaagggca agatcgcgag cgtcgcggtg aaggccacgg actccgccgg catctccgac
     2101 gccgccatcg acaagatccc cgccgccatc gtcaaggccc agagcgccga ggtcgacgcc
     2161 gtcgccggcg cctccgagac cagcgaggcc atcaaggccg ccgtcgccaa ggcactcgcc
     2221 ggcgcgaccc tctaccccgt cgcctccacg atgaagaccg acgtggtcgt cttgggcggc
     2281 ggcaacgcgg gcctcgccgc ggccgtcgag gccgccaacg cgggcgccaa ggtcgtcctc
     2341 gtggagaaga tggccttcct gggcggcaac tcgatccgcg ccggcggcgc ctacaacgcc
     2401 gtcgaccccg agcgccagga gaagcagggc atcgtcgact ccatcgacaa gcactacgag
     2461 cagaccctct cgggcggcga cggcaagggc gaccccgccc tggtgcgcac cctcgtcgaa
     2521 ggctctccca agggcctcaa gtggctcgag ggcatgggca tgaagttcaa cgacaaggtc
     2581 ttcaccgtcc tcggcgccct ctggccccgc agccactccc ccgtcgagcc cgtcggcacc
     2641 ggcttcatca agaccctcaa ggccgccgcc gagggcaagg gcgtgaagtt cctcctcgag
     2701 accaaggccg accagatcat ccgcgaggac ggcagggtca agggcgtcgt cgtcgtggac
     2761 gcggccggca agcggatccg gatcaacgcc tcccgcggcg tcgtcctggc cacgggcggc
     2821 ttcgcggcca acaaggagat gcgccaggcc tacgatcccc gcatcacccc gaacctcggc
     2881 acgacgaacc accccggcgc cactggcgac ggcgtcgtca tggccgagaa gatcggcgcc
     2941 gccctcgtgg gcatggagta catccagatg ctccccctcg gcgatcccaa gaccggcg
//
LOCUS       DAOOSJ010000286         2970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_22166_length_2970_cov_4.282333, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000286 DAOOSJ010000000
VERSION     DAOOSJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22166_length_2970_cov_4.282333"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLG14_11380"
     CDS             <1..377
                     /locus_tag="PLG14_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49778.1"
                     /translation="HRWLESDFRRQVSMAFTEDGRYLAYEIPSGLGVYDREKRAESKI
                     AAAALSGVGLSSPERGLLLALEGGGASRGLLVASPSGRRLFTAPFAAAESFLARRGDA
                     LFLGMADGSGSASILRMDFKEE"
     gene            377..1282
                     /locus_tag="PLG14_11385"
     CDS             377..1282
                     /locus_tag="PLG14_11385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX49779.1"
                     /translation="MERRSRPQGKAPALLLACAALAALASGASALDWPLADPKPAATF
                     GTAAKGRFVSGVLLASEGGLVRAAGDGELVYAFDGSSPTAVSALPSTLGSFAVVEHER
                     GMAGLYAGLAPGSVSSYLTKTKAGSILGTAGSSGWAEGQGLLFAVYDRGAERWVNPLL
                     LLPALKDKTQPLIRSAALARGGKSYALGETRSVPQGEYAIAVDVADPLDALWSAGSPA
                     PYYLRLLVDGAKIAELEYDVMEGRSGRLVLFPQAPKSRADYLLPDGRALVAERFFRRG
                     RSVIEVLVRDYAGNERGASWSIAVE"
     gene            1279..2142
                     /locus_tag="PLG14_11390"
     CDS             1279..2142
                     /locus_tag="PLG14_11390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX49780.1"
                     /translation="MKTFATRPRPEPLSPRPCPLCGGSRLAPAWDCGAFRFVRCLSCG
                     LVQQNPQPEASAVLARYDESYLAYEEERQFDYRDLELLALADLRFEEAAAPLRREAED
                     GGRKPRVLDVGCATGALLAKLREDGWDCVGVEACAPAAAYGRRLFGLDIRATTLEEAR
                     LPAASFEAVHASHLIEHLNEPGAFLDEAARLLVPGGLLVLATPNADGLQARLLGPEWR
                     SAIYDHLCLFSRRNLPAFLAAKGFAVERLVTWGGWAAGLEPAFVKRPLDRAAKRLGFG
                     DVMAIMARSAT"
     gene            2229..>2970
                     /locus_tag="PLG14_11395"
     CDS             2229..>2970
                     /locus_tag="PLG14_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOX49781.1"
                     /translation="MEAAQKVLFVDASTGFYRVKRYKLGEYFGPVDLGLHISGRTMSL
                     NFGTGLFAGSILPGSNRMVFTGFSPCWRGFYISSMGGAGLVFDNLGINMVSLVGKAAM
                     PSILYLNRLHGEEIEVELEPVDLTAVWAGRDGCADGGPGGGSDHSPGSARGSSGTRES
                     GEARSPGLARAGAYALLDHALARYGRRYEADYRAFAVGPAAQASDFGAILSAPVKDGK
                     ATDVDTWAGRGGLGSKMLQEHGIAAVVYG"
BASE COUNT          380 a         1167 c          985 g          438 t
ORIGIN      
        1 accatcgctg gctcgagtcg gacttccggc gccaggtctc catggccttc accgaagacg
       61 gccgctacct cgcctacgag atcccttccg gcctcggcgt ctacgaccgc gaaaaacgag
      121 ccgagtcgaa gatcgcggcc gcggccctct ccggcgtcgg cctctcctcc cccgagcgcg
      181 gcctcctcct cgccctggag ggcggcggcg cgtcgagggg gctcctcgtc gcctcgccct
      241 ccggccgccg cctcttcacc gcccccttcg ccgccgccga gagcttcctc gcgcgccgcg
      301 gcgacgccct tttcctcgga atggccgacg gctccggctc ggcctcgatc ctcaggatgg
      361 atttcaagga ggagtgatgg agcgacgatc aaggccccag ggaaaggctc cggcccttct
      421 cctcgcctgc gcggccctcg cggccctcgc ctccggcgcc tccgccctcg actggcccct
      481 ggccgacccc aagccggccg ccaccttcgg cacggcggcc aaggggcgct tcgtgagcgg
      541 cgtcctcctc gcctccgagg gaggcctcgt gcgcgccgcg ggggacggcg agctcgtcta
      601 cgccttcgac ggctcctcgc cgacggcggt ctccgcgctt ccctcgacgc tgggatcctt
      661 cgccgtggtc gagcacgagc ggggcatggc cggcctctac gcggggctcg cccccggctc
      721 cgtctcctcc tacctcacga agacgaaggc ggggagcatc ctggggacgg ccggctcttc
      781 gggctgggcg gagggccaag gcctcctctt cgccgtctac gaccgcggcg ccgagcgctg
      841 ggtgaatccc ctcctactcc tccccgcgct gaaagacaag acccagcccc tcatccgctc
      901 cgcggccctc gcgcggggag ggaagagcta cgccctgggc gagacccggt cggtccccca
      961 gggcgagtac gccatcgcgg tggacgtcgc cgatcccctg gacgccctct ggtcggccgg
     1021 aagcccggct ccctactacc tccgcctcct cgtcgacgga gcgaagatcg ccgagctcga
     1081 gtacgacgtc atggagggcc gctcggggag gctcgtcctc ttccctcagg cgcccaagag
     1141 ccgcgccgac tacctcctcc ccgacggccg cgccctcgtc gccgagcgct ttttcaggag
     1201 gggccgctcc gtgatcgagg tcttggtccg ggactacgcg ggcaacgagc gcggcgcctc
     1261 ctggtcgatc gcggtggaat gaagaccttc gcgaccaggc cccggcccga gcccctctcg
     1321 ccccgcccct gtcccctctg cggcggctcg cgcctcgccc cggcctggga ctgcggcgcc
     1381 ttccgcttcg tccgctgcct ctcctgcggc ctcgtccagc agaatcccca gcccgaggcc
     1441 tcggccgtcc tggcccgcta cgacgagagc tacctcgcct acgaggagga gcggcagttc
     1501 gactaccgcg acctcgagct cctcgccctg gccgacctcc gcttcgagga ggccgcggcc
     1561 cccttgcggc gggaggccga agacggcggg aggaagcccc gcgtcctcga cgtgggctgc
     1621 gcgacggggg ccctcctcgc gaagctgcgg gaagacggct gggactgcgt cggcgtcgag
     1681 gcctgcgccc cggccgccgc ctacggccgg aggctcttcg gtctcgacat ccgcgccacg
     1741 accctggagg aggcccgcct gcccgcggcc tccttcgagg ccgtccacgc ctcccacctc
     1801 atcgagcacc tcaacgagcc cggggccttc ctggacgagg ccgcgcggct cctcgtcccc
     1861 ggcggcctcc tcgtcctcgc gacgcccaac gcggacggcc tccaggcccg cctcctcggc
     1921 cccgagtgga ggagcgcgat ctacgaccat ctctgcctct tctcgcggcg gaacctgccc
     1981 gccttcctgg ccgcgaaggg cttcgccgtg gagcggctcg tcacctgggg aggctgggcc
     2041 gcgggccttg agcccgcctt cgtgaagcgc cccctggacc gggccgcgaa gcgcctgggc
     2101 ttcggcgacg tcatggccat catggcgcgg agcgccacgt gaacgatcgc cgcgacgggg
     2161 cagggcgatc atggcgcggc ttgatcggcg ggccgggaag tcctagagtc taggtcgagg
     2221 agcaacagat ggaagcagcc cagaaagtcc tcttcgtcga cgcgtccacg gggttctatc
     2281 gggtcaagcg ctacaagctc ggcgagtact tcggtcccgt cgacctaggc ctccacatct
     2341 cggggcggac catgagcctc aacttcggca cgggcctctt cgcgggctcc atcctgccgg
     2401 gctccaaccg catggtcttc acgggcttct cgccctgctg gcggggcttc tacatctcca
     2461 gcatgggcgg cgccggcctc gtcttcgaca acctcggcat caacatggtg agcctggtcg
     2521 gaaaggccgc catgccctcc atcctctacc tgaaccgcct ccacggcgag gagatcgagg
     2581 tcgagctcga gcccgtcgac cttacggccg tctgggccgg ccgggacggc tgcgcggacg
     2641 gcggcccggg cggcggctcg gaccatagcc cgggttccgc gagaggatcg agcggaaccc
     2701 gagagtctgg cgaagccaga agcccgggcc ttgcccgggc gggggcctac gctctcctcg
     2761 accacgccct ggcgcgctac ggcaggcgct acgaggccga ctaccgcgcc ttcgcggtcg
     2821 gcccggccgc ccaggccagc gacttcggcg ccatcctctc cgcgccggtg aaggacggga
     2881 aagcgacgga cgtcgacacc tgggcgggcc gcgggggcct cggctccaag atgctccagg
     2941 agcacggcat cgccgccgtc gtctacggcg
//
LOCUS       DAOOSJ010000287         2902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23029_length_2902_cov_6.372673, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000287 DAOOSJ010000000
VERSION     DAOOSJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23029_length_2902_cov_6.372673"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLG14_11400"
     CDS             <1..474
                     /locus_tag="PLG14_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HOX49782.1"
                     /translation="AGGFACGVLLALVPPGNLLWILLFAVTYFTKINYGMQILVTLLF
                     MALRPLVAPALDAVGWAFLNLEPLQGAFTTLYNLPLAPLTRFNNSIVAGGLLAGLALW
                     LPLFFAMRAFVAFYRARLAPKVAESRLYKAFRKLPLVQAVSKAASLASKAAGALE"
     gene            489..2552
                     /locus_tag="PLG14_11405"
     CDS             489..2552
                     /locus_tag="PLG14_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49783.1"
                     /translation="MNEQKKATRAKKPPKPAPIFRKPIPAKAFEKKYLRLVEQAKDRE
                     FLESAFRQEGGSFRLKEGLDREALVRLNRLGKAIKGNRGLVKTGPLLLAAILGTGLAV
                     FGLFFMNPSLERATERALEAVFGARAEVSGLRLDLGRLRIAMASVAVADRDEPMTNLF
                     ETGRLELRLDLASLVRGRVYVEEATAASIALGTARSVSGALPDSPARPKAAKPAKAAA
                     PPLLDFGNFDAKALLEREKAKLKSGAAYDEAGKAYAESAARWKGRAESSKKAVASLSS
                     SSKAVLAIDPKAIKTAADAASALAAVKALAGDAESVAKESGAVAAGLKADSETAAGLE
                     KAARKALAEDTAYLKSLVDPKSGAARGALEPSVREILSDKAERWLYYGARGLEVAGRL
                     KAGSGSGKADKAGKKQAAPAPRGRDVVYPSAALPRFRLGLLSSSFVSGGSEWKLELRE
                     LSTEPDLVASPASLDFSVSDGSSLVAAKAVADLRGEKEDYSLDAKGEGLPLDLGSALE
                     SLGLGGFTGKASGRLELSGSKDGALRAKGGLDVAEARAAKPEGTLGEALAGAIASVDA
                     VRLALAYERPAGGEGKLSLRTNLDELVAKAVEAVAARYAKQASASLDAALRDYAGKEL
                     EGKLASKGELDAALASAKGDEAAAASTRKSLDAKRASLEARAKEIGTAALGGLGGVTV
                     PKLKP"
BASE COUNT          410 a         1109 c          996 g          387 t
ORIGIN      
        1 gcgggcggct tcgcctgcgg cgtcctcctc gccctggtgc cgcccgggaa cctcctctgg
       61 atcctccttt tcgcggtcac ctatttcacc aagatcaact acgggatgca gatcctcgtg
      121 accctcctct tcatggccct gcgccccctt gtcgcgccgg ccctggacgc ggtgggctgg
      181 gccttcctca acctcgagcc cctccagggg gccttcacga ccctctacaa tctgcccctg
      241 gccccgctca cgcgcttcaa caacagcatc gtcgccggcg gcctcctcgc gggactggcc
      301 ctctggctgc cgctcttttt cgcgatgcgc gccttcgtcg ccttctaccg cgcgaggctc
      361 gcgcccaagg tggcggagtc gcggctctac aaggccttcc ggaagctgcc cctggtccag
      421 gcggtctcca aggcggcctc cctggcgtcc aaggccgccg gagccctgga atgaggaagg
      481 ggacgaccat gaacgagcag aagaaagcga cgagggccaa gaagccgccc aagccggcgc
      541 cgatcttcag gaagcccatc cccgcgaagg ccttcgagaa aaagtacctc cgccttgtcg
      601 agcaagcgaa ggaccgcgag ttcctggagt ccgccttcag gcaggagggc ggctcgttcc
      661 ggctcaagga gggcctggac cgggaggccc tggtccggct caaccggctc gggaaggcga
      721 tcaaggggaa ccgcggcctc gtgaagaccg gccccctcct cctcgcagcc atcctcggga
      781 cgggactcgc cgtcttcggg ctctttttca tgaatccgag cctggaacgg gccacggagc
      841 gggccctgga ggcggtcttc ggcgcgcggg ccgaggtctc cggcctccgc ctcgacctgg
      901 gacggctcag gatcgcgatg gcctccgtgg ccgtcgcgga ccgggacgag ccgatgacga
      961 acctcttcga gaccggccgc ctcgagctcc gcctggacct cgcctccctg gtccggggcc
     1021 gcgtctacgt cgaggaggct accgccgcct ccatcgccct cgggacggcc cgttccgtct
     1081 cgggcgccct tcccgactcc cccgcgaggc ccaaggccgc gaagccggcc aaggccgccg
     1141 cgccgcccct cctcgacttc ggtaacttcg acgccaaagc cctcctcgag cgggagaagg
     1201 cgaagctcaa gagcggagcc gcctacgacg aggcgggcaa ggcctacgcc gagtcggcgg
     1261 cgcgctggaa gggcagggcc gagtcctcca agaaggcggt cgcctcccta agctcctcct
     1321 ccaaggccgt cctcgccatc gatcccaagg ccatcaagac ggccgccgac gcggcgagcg
     1381 ccctcgccgc cgtcaaggcc ctcgcgggcg acgcggagag cgtcgcgaag gagtccggcg
     1441 cggtcgcggc cggcctcaag gccgactcgg agacggccgc gggcctcgaa aaggccgccc
     1501 gcaaggccct ggccgaggac acggcctacc tcaagtccct ggtcgacccg aagagcggcg
     1561 cggccagggg ggccctggag ccctccgtcc gcgagatcct ctcggacaag gccgagcgct
     1621 ggctctacta cggcgcccgc ggcctggaag tcgccgggcg cctcaaggcc ggctcaggct
     1681 ccggcaaggc cgacaaggcc ggcaagaagc aggcagcgcc ggccccccgc gggcgggacg
     1741 tggtctatcc ctcggcggcc ctgccgcgct tccgcctcgg cctcctctcc tccagcttcg
     1801 tctccggcgg gagcgagtgg aagctcgagc tccgcgagct ctccaccgag cccgacctcg
     1861 tggcctcgcc cgcgagcctc gacttctccg tctcggacgg ctcctccctg gtcgcggcca
     1921 aggccgtcgc cgacctgcgg ggggagaagg aagactactc gctggacgcg aagggcgagg
     1981 gcctccccct cgacctgggc tccgccctcg agtccctggg cctgggcgga ttcacgggca
     2041 aggcctcggg acggctcgag ctctcgggct ctaaggacgg ggccctgagg gccaagggcg
     2101 gcctggacgt cgcggaagcc cgggcggcga agcctgaagg caccctgggc gaggccctgg
     2161 ccggcgcgat cgcctccgtg gacgccgtgc gcctcgccct ggcctacgag cggccggcgg
     2221 gaggggaggg gaagctctcc ctgcggacga acctcgacga gctcgtcgcg aaggccgtgg
     2281 aggccgtcgc cgcccgctac gccaagcagg cctccgcctc cctggatgcc gccctgcgcg
     2341 actacgcggg gaaggagctc gagggcaagc tcgcctccaa gggagagctg gacgcagccc
     2401 tggcctcggc caagggcgac gaggccgccg ccgcctccac caggaagtcc ctggacgcga
     2461 agcgggccag cctggaggcc agggccaagg agatcgggac ggccgccttg ggagggctcg
     2521 gcggcgtcac ggtgccgaag ctcaagccct agcggagagg ccacgcttca ggcggcaggg
     2581 gcgaccgcgc ctcccgccta agcaggccag gccgccccgc cgtcggcgcc ctggccgccc
     2641 atgcgtgggc catgccggtt gtgccgccgc gcccgctccg gcgcagggca tgccggcgcg
     2701 gccgccgtct ttccggccgc cgcgcccgct ccggcgcagg cgccgattag gagagcgtca
     2761 cgcggccgcg cctttgccca gaccgcccag gcgcgcgccc gttggcgcgg ccgccgcatt
     2821 tccgggcggg tggcctcatt atccttccgc cgccgcggaa ggccgtgcta tgcttccccc
     2881 atgccgcgct tcacgttgaa cg
//
LOCUS       DAOOSJ010000288         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23146_length_2892_cov_5.633768, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000288 DAOOSJ010000000
VERSION     DAOOSJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23146_length_2892_cov_5.633768"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1045
                     /locus_tag="PLG14_11410"
     CDS             95..1045
                     /locus_tag="PLG14_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49784.1"
                     /translation="MRAFSFLPLLLCAAIAAAAPGAGAEPLPRVAVLPFEPGKLTASD
                     AAALTLFAETALQNTGRCVIVEQSQVDKILKAHQYALADFNDPAKAVEIGKLVPADHI
                     VLGTAGILAGKYFLNLKLVDLRTGTNEAARAATAASLEALAEAVGGLAAALVGGSPPR
                     SAPPAAGASPGFVPGPAPGVAPSAPGLSPPQAAMPVILGAGMGFFESGAGIPPLGSRT
                     YSYAFPRGSARFINWEITIDVAPPSGPLSFPLSIVYRRPDGGVHASWEKEARLDRGST
                     WVKLWDGWGSAAPGTWAAGAYRVEAYSGGTLLAWAVFELY"
     gene            1098..1427
                     /locus_tag="PLG14_11415"
     CDS             1098..1427
                     /locus_tag="PLG14_11415"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOX49785.1"
                     /translation="MQRSLVIWLFFAVVVALFAVVNSAPVAVNLLFGQFEVSLALLVL
                     VPLLVGAALGYTLDLPRRLKARSRSKELERRLAEAEAGKAEAAAKESEAEKAPSAARE
                     AAPGEAK"
     gene            1595..>2892
                     /locus_tag="PLG14_11420"
     CDS             1595..>2892
                     /locus_tag="PLG14_11420"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOX49786.1"
                     /translation="MIRPEEVRHEALKAWVAGIAELCKPDRVHWCDGSEEENERVCEG
                     LVESGTFIRLDPKKRPNSFLARSDPGDVARVEDRTFICSRRKVEAGPTNNWKDPKEMK
                     AILNGLFDGCMRGRTMYVIPFCMGPLGSPLSHLGVEITDSGYVVANMRIMTRMGRPAL
                     EALGTEGEFVPCVHSVGAPLEPGEKDVPWPCDRENKYIVHFPEERAIWSYGSGYGGNA
                     LLGKKCFALRIASVIAREQGWLAEHMLILGVQSPEGEKTYVAAAFPSACGKTNFAMLI
                     PPEGFEGWKVTTVGDDIAWIKPDKTGQTRAINPEAGYFGVAPGTSEKSNPNAMATIRA
                     NTIFTNVALTPDGDVWWEGMTKVPPEGLVDWQGKPWSPEGGKPAAHPNARFTAPAKQC
                     PSIDPDWENPSGVPIKAFIFGGRRSALFPLVYQSFNWIYGV"
BASE COUNT          445 a         1078 c          951 g          418 t
ORIGIN      
        1 tttccgatga ggcaacgacc ccgctcgggt ttgaatttgg atgaggcaat tcgggcgatt
       61 gcgcccggcc gccggcggtc ctatagttga aggcatgagg gccttctcct tccttcccct
      121 cctcctttgc gccgccatcg cggcggccgc gccgggagcg ggcgccgagc ccctgccgcg
      181 cgtcgccgtc ctccccttcg agccgggcaa gctgaccgcc tccgacgcgg cggccctgac
      241 cctcttcgcg gagacggccc tccagaacac gggccgctgc gtcatcgtcg agcagtccca
      301 ggtggacaag atactcaagg cccaccagta cgccctggcc gacttcaacg acccggccaa
      361 ggccgtcgag atcggaaagc tcgtccccgc cgaccacatc gtcctcggaa ccgcgggcat
      421 cctcgcgggc aagtacttcc tgaacctcaa gctggtcgac ttgcggacgg gcacgaacga
      481 ggcggcccgc gccgcgacgg ccgcgagcct cgaggcactg gccgaagcgg tcggcggcct
      541 ggccgcggcc ctggtcggcg gttcgccgcc gcgaagcgcg ccgccggccg cgggagcctc
      601 gccgggcttc gtcccgggtc ccgcgccggg cgtcgctccc tcggccccgg gcctgtcgcc
      661 gccccaggcg gccatgcccg tcatcctggg cgccggcatg ggcttcttcg agagcggggc
      721 gggcatcccg cctctcgggt cgcggaccta ctcctacgcc ttcccccgcg gctcggcacg
      781 cttcatcaac tgggagatca ccatcgacgt ggcgcctccc tccgggcccc tctccttccc
      841 cctctcgatc gtctatcgcc ggcccgacgg cggcgtccac gcctcctggg agaaggaggc
      901 gaggctggac cgcggctcca cctgggtgaa gctctgggac ggctggggca gcgccgcccc
      961 cggcacctgg gcggcgggcg cctaccgcgt cgaggcctac tcaggcggga ccctcctcgc
     1021 ctgggccgtc ttcgagctat actgaatcgt ttccggacgg cggacattcc cgccgcctgg
     1081 cggcaggagg attttccatg cagcgcagtc tcgtcatctg gctcttcttc gcagtcgtcg
     1141 tcgccctctt cgccgtggtg aattcggctc ccgtggcggt caacctcctc ttcggccagt
     1201 tcgaggtgtc cctcgccctc ctggtcctcg tgcccctcct cgtcggagcc gccctgggct
     1261 acaccctcga cctgccgcgc cggctcaagg cccggagccg gagcaaggag ctcgagcgga
     1321 ggctcgccga ggccgaggcc gggaaggccg aggccgccgc caaggaatcc gaagccgaga
     1381 aggcgccgtc cgccgcgcgg gaagccgcgc ccggcgaggc gaagtagagc cgctcctcct
     1441 ccggaatcgc ccatcgctcc cgcccgacgc cgctcctcgg ttgccaaggc ccctagcgcc
     1501 cctcccctcc caaggttttc caccgcgaaa gggaaaaccg atatatgatg gacgcgacgc
     1561 gcacgcacag cgcgaaccga aaaaggaggg aacgatgata cgaccggagg aggtccggca
     1621 cgaggcgctc aaggcctggg tggccggcat cgccgaactc tgcaagcccg accgcgtcca
     1681 ttggtgcgac ggctcggagg aggagaacga gagagtctgc gaggggctcg tcgagtcggg
     1741 gaccttcatc aggctcgacc cgaagaagcg ccccaacagc ttcctggcca ggtccgaccc
     1801 gggcgacgtc gcgcgggtgg aggaccggac cttcatctgc agccgccgca aggtggaggc
     1861 gggccccacc aacaactgga aggacccgaa ggagatgaag gccatcctca acggcctctt
     1921 cgacggctgc atgcgcggcc ggaccatgta cgtgatcccc ttctgcatgg ggccgctcgg
     1981 ctcccccctc tcccacctgg gcgtggagat caccgactcg ggctacgtcg tggcgaacat
     2041 gcggatcatg acgcgcatgg gccgccccgc cctggaggcc ctcgggacgg agggcgagtt
     2101 cgtcccctgc gtccactccg tcggcgcgcc cctcgagccc ggcgagaagg acgtcccctg
     2161 gccctgcgac cgggagaaca agtacatcgt gcacttcccc gaggagcgcg cgatctggtc
     2221 ctacggctcg ggctacggcg gcaacgccct gctcggcaag aagtgcttcg cgctccgcat
     2281 cgcctcggtg atcgcgcgcg agcagggctg gctggccgag cacatgctca tcctgggagt
     2341 ccagtcgccg gagggcgaga agacctacgt cgcggcggcc ttcccgagcg cctgcggcaa
     2401 gaccaacttc gcgatgctca tcccgcccga gggcttcgag ggctggaagg tcacgacggt
     2461 gggcgacgac atcgcctgga tcaagcccga caagacgggg cagacccgcg cgatcaatcc
     2521 cgaggccggc tacttcggcg tcgcgccggg cacgagcgag aagtccaacc cgaacgcgat
     2581 ggcgacgatc cgcgccaaca cgatcttcac gaacgtggcc ctgacgcccg acggcgacgt
     2641 ctggtgggag ggcatgacca aggtcccgcc cgagggactc gtcgactggc agggcaagcc
     2701 ctggtccccg gaaggcggca agcccgcggc ccatccgaac gcgcgcttca cggcgcccgc
     2761 gaagcagtgc ccctcgatcg accccgactg ggagaacccg agcggcgtcc cgatcaaggc
     2821 cttcatcttc ggcggcaggc gcagcgccct cttccccctc gtctaccagt ccttcaactg
     2881 gatctacggc gt
//
LOCUS       DAOOSJ010000289         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23293_length_2880_cov_3.806372, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000289 DAOOSJ010000000
VERSION     DAOOSJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23293_length_2880_cov_3.806372"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLG14_11425"
     CDS             <1..293
                     /locus_tag="PLG14_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49787.1"
                     /translation="LWFYSGPEGQRLRFERGGGQGAAIDPRRLGLYVDPLWAKAYELL
                     SAAPLVDIIDFRMETPFVNAGLKELLAGRKRPEALAQEIEAWVARNDPNRRR"
     gene            complement(392..826)
                     /locus_tag="PLG14_11430"
     CDS             complement(392..826)
                     /locus_tag="PLG14_11430"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HOX49788.1"
                     /translation="MKWITRSHVHVDRVACPWLIRRFVDNEAEFLFVPASSLAEAAER
                     EGAIPFDAPGVELGHRDGKCSFETIVEVYGLKDPALLLLARIVGCADTGRLAEHPVAA
                     GLEALAVGYSLRFPDDEENLARQFEVYDSLYAWCRLEAARAR"
     gene            1036..>1310
                     /locus_tag="PLG14_11435"
     CDS             1036..>1310
                     /locus_tag="PLG14_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49789.1"
                     /translation="MAQQNEIAVKIPEEDLAEIRGAIKVLQKKLAPHLASLSPQDRLE
                     LPKMGEKTYTFVQKAYEYGMKNKELAPPYLDFGAMETDVKAVGLLRDL"
     assembly_gap    1311..1410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1411..1658
                     /locus_tag="PLG14_11440"
     CDS             <1411..1658
                     /locus_tag="PLG14_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49790.1"
                     /translation="DFGAMETDVKAVGLLRDLTQSLNPVLDALHDSLYLSGSEAYQGA
                     LLFYGSVKAAAKAKAPNAGSIYEDLSSRFPGGPRKKA"
     gene            complement(2012..>2880)
                     /locus_tag="PLG14_11445"
     CDS             complement(2012..>2880)
                     /locus_tag="PLG14_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49791.1"
                     /translation="GSPAKEAHPDVYDLDEAPDPEEYEDAYIRHSRPGLPLPVMGRPA
                     FRIPGRLLPKGDYRVTVARYPLAAALAAAKARGVTLTELATAVYVEALQEIWLESGPK
                     ERRRDEIAVEVPVDMRRFFPTKTNRNFTLFTIVGEDLRLGRRSFEDILERTRCRFRLE
                     CDEASISRQITRNVHGLKHPLVRALPLRLKDIGAKILFRTLGEDYVSGIVTNLGAVDF
                     PKGVAERVLRFDLAQPPSDVTRANVAIHSYSGELYVTVCSLAEGRRMEGLVYGRLASL
                     GLEAPLECNLED"
BASE COUNT          462 a          925 c          969 g          424 t
ORIGIN      
        1 ggctctggtt ctactcgggc cccgagggcc agcggctgcg cttcgagcgc ggcggcggcc
       61 aaggcgccgc catcgacccc cgccgcctcg ggctctacgt ggaccctctc tgggccaagg
      121 cctacgaact cctctccgcc gcccccctcg tcgacatcat cgacttccgg atggagacgc
      181 ccttcgtcaa cgcgggcctg aaggagctcc tggcggggcg gaagcgtccg gaggccctgg
      241 cccaggagat cgaggcctgg gtcgcgcgca acgaccccaa ccgcaggcgc taggcggccg
      301 gacgcctaca aaagaatgac cgaagcccgc cgggtcccgg cgggcttctt ttattccagg
      361 ttgagcgcag gccgcctccc cgcggaaggc ctcaacgcgc gcgcgccgcc tcgagcctgc
      421 accaggcgta gagggagtcg tagacctcga actgccgcgc taggttctcc tcgtcgtcgg
      481 ggaagcggag cgaatagccg acggcgagcg cctcgaggcc ggcggcgacg gggtgttcgg
      541 cgagccggcc cgtgtcggcg cagccgacta tcctcgcgag gaggaggagg gcgggatcct
      601 tgagcccgta gacctcgacg atggtctcga aggagcactt cccgtcccga tggcctagct
      661 cgacgcccgg cgcgtcgaag gggatcgcgc cctccctctc cgcggcctcc gccaggctcg
      721 aggcggggac gaagaggaat tccgcctcgt tgtcgacgaa gcgcctgatg agccagggac
      781 aggctacccg atcgacgtgg acatgcgatc tcgtgatcca tttcatgggg actccttcgc
      841 ggactcaagc gggatggccg ctggtctcga tactagcccg ccttcgggac ggccggaaga
      901 gtcggagccc gccaagtgga gctttttgcc cctccctacg tcccctttgg gcgattgtct
      961 acaggcgatt cccttgcatt ccgctacctg aggcgttttc cttgacgtat cgcgtatttt
     1021 ttgaaggagg gcgacatggc ccaacagaac gaaatcgcgg tcaagattcc cgaggaggac
     1081 ctggcggaga tccgcggcgc catcaaggtc ctccagaaga agctcgcgcc ccacctcgcg
     1141 agcctctcgc cccaggacag gctggagctc cccaagatgg gcgagaagac ctacaccttt
     1201 gtccagaagg cctacgagta cgggatgaaa aacaaggagc tggccccgcc ctacctggac
     1261 ttcggcgcca tggagaccga cgtgaaggcc gtgggcctcc tccgggacct nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggacttcgg cgccatggag accgacgtga
     1441 aggccgtggg cctcctccgg gaccttacgc agagcctcaa tcccgtcctc gacgctctcc
     1501 acgacagcct ctacctctcg gggagcgagg cctaccaggg cgccctcctc ttctacggca
     1561 gcgtgaaggc ggccgccaag gccaaggccc ccaacgccgg aagcatctac gaggatctct
     1621 cgagccgctt cccgggcggt ccgaggaaga aggcctagac caaggccgcg gaccccgcct
     1681 ggcgtagcgc ctggcaccgc cgcggccttg tcgcgcctac aagcaagccg cccgcgggaa
     1741 gggacatcct tccacgcggg cggctgttct atcttggagg caggaacttg aggctcgcag
     1801 gcaggatgag ccgccacgga catggaacat cccacccagc ggatggcgaa gtccatcttc
     1861 cggatggaaa ttcctatgct ggggatagga cgagccatgg caaacatggg aattccctgc
     1921 gctcaggcag gcggtgccat ctcccaggag ggaattcctg cctcgccgac gggacaagcc
     1981 gctcaagcga cgggaattcc cgcttcgggc ctcagtcctc caggttgcac tccaggggcg
     2041 cctcgaggcc cagggaggcg aggcggccgt agacgaggcc ctccatgcgc cggccctcag
     2101 ccaggctgca gacggtgacg tagagctcgc cagagtagct gtggatcgcg acgttggcgc
     2161 gggtcacgtc ggagggcggc tgggccaggt cgaagcggag gacgcgctcg gcgacgccct
     2221 tggggaagtc cacggcgccc aggttcgtga cgatgccgga gacgtagtcc tcgcccaggg
     2281 tcctgaagag gatcttcgcc ccgatatcct tgagcctgag gggcagggcc cggaccaggg
     2341 ggtgcttgag gccgtggacg ttgcgggtga tctgccgcga gatcgaggcc tcgtcgcact
     2401 ccaggcggaa gcggcagcgc gtgcgctcca ggatgtcctc gaagctccgc cggcccaggc
     2461 gcaggtcctc gcccacgatc gtgaagaggg tgaagttccg gttcgtcttc gtggggaaga
     2521 agcggcgcat gtccacgggg acctcgacgg cgatctcgtc gcggcggcgc tccttcggtc
     2581 ccgactcgag ccagatctcc tgcagggcct ccacgtagac ggccgtggcg agctccgtga
     2641 gggtgacgcc ccgggccttc gcggcggcga gggccgcggc gaggggatag cgggcgacgg
     2701 tgacgcgata gtcgcccttg gggaggagcc gcccggggat gcggaaggcg ggccggccca
     2761 tcacgggcag ggggaggccg ggccgggagt ggcggatgta ggcgtcctcg tactcctcgg
     2821 gatcgggggc ctcgtccagg tcgtagacgt cggggtgggc ctccttggcc gggctcccgg
//
LOCUS       DAOOSJ010000290         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23340_length_2876_cov_6.753633, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000290 DAOOSJ010000000
VERSION     DAOOSJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23340_length_2876_cov_6.753633"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="groEL"
                     /locus_tag="PLG14_11450"
     CDS             complement(<1..1021)
                     /gene="groEL"
                     /locus_tag="PLG14_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOX49792.1"
                     /translation="MAKQLLFSEDARRKLLVGVETISRAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAKEVELEDPYENMGAQLLKEVATKTNDIAGDGTTTATVLAYSIVKEG
                     LKSVAAGMDPMGLKRGIDKAVEIAVEEIKKNSKEIKEKEEIADVASVSANNDEEIGKQ
                     IADAMEKVGKDGVITVEESKTMDTTIDFVEGMQFDRGYSSPYFVTNRDTMTAVLENPY
                     ILIHDKKISNMKDLLPMLEKIAQAGKPLLIIAEDIEGEALATLVVNHLRGTLTVCAVK
                     APGFGDRRKAMLEDIAILTGGEVISEEIGLKLENTAVGQLGTAKTVKVDKDNTTIING
                     AGKQKD"
     gene            1225..1659
                     /locus_tag="PLG14_11455"
     CDS             1225..1659
                     /locus_tag="PLG14_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOX49793.1"
                     /translation="MDEIALMESIASSWRRLSAKRIRPFEISLLQYRLILLARRKGSV
                     SLSQAAEGLGCDRPGLTLLARKCVRSGWLRRRRSAADRRSHRLELTGEGEELLDAIEA
                     AQASPSAAAADPLDVLAPAEREALAGMLRRVRDRAEELRAGR"
     gene            1820..2686
                     /locus_tag="PLG14_11460"
     CDS             1820..2686
                     /locus_tag="PLG14_11460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX49794.1"
                     /translation="MRETFKIIYSDGDILVVEKPAGLLSVPDRYEPEVPALVTELRKR
                     LAEGLLLPLAKAEGAAEGAGPAAAEASGQKAAETAQPVGQADPARGAELLVVHRLDKD
                     TSGLLIFARNAEAHRRLNGAFGTGAVKKRYRAIVKGSPNWEETTCELPLHVDADRSHR
                     TIIDGHHGKESRTVFAVLDRYRGAGGFSAALVEARPETGRTHQIRVHLASLGHACLCD
                     PLYGDGEPLYLSKVKGKWKGDEWSDRPLLSRTALHAFSLDFDHPMTGEPLHFEAAEPK
                     DFRAARIQLEKL"
BASE COUNT          394 a          995 c          977 g          510 t
ORIGIN      
        1 tgtccttctg cttgccggcg ccgttgatga tggtggtgtt gtccttgtcc accttcacgg
       61 tcttggcggt gccgagctgg ccgacggcgg tgttctcgag cttgaggccg atctcctcgg
      121 agatcacctc gccgcccgtg aggatcgcga tgtcctcgag catggccttg cggcggtcgc
      181 cgaagccggg ggccttcacg gcgcagacgg tgagggtgcc gcggaggtgg ttcacgacga
      241 gggtggcgag ggcctcgccc tcgatgtcct cggcgatgat gaggaggggc ttgccggcct
      301 gggcgatctt ctcgagcatg gggaggaggt ccttcatgtt cgagatcttc ttgtcgtgga
      361 tgaggatgta ggggttctcg agcacggccg tcatcgtgtc gcggttggtc acgaagtagg
      421 gggaggagta gccgcggtcg aactgcatgc cctcgacgaa gtcgatcgtg gtgtccatgg
      481 tcttggactc ctcgaccgtg atgacgccgt ccttgccgac cttctccatg gcgtcggcga
      541 tctgcttgcc gatctcctcg tcgttgttgg cggagacgga cgcgacgtcg gcgatctcct
      601 ccttctcctt gatctccttc gagttcttct tgatctcctc gacggcgatc tccacggcct
      661 tgtcgatgcc gcgcttgagg cccatggggt ccatgccggc ggcgacggac ttgaggccct
      721 ccttgacgat ggagtaggcg aggacggtgg cggtcgtggt gccgtcgccg gcgatgtcgt
      781 tggtcttggt cgcgacttcc ttgaggagct gggcgcccat gttctcgtag gggtcctcga
      841 gctcgacctc cttggcgacg gagacgccgt ccttggtgac ggtgggagcg ccgaacttct
      901 tgtcgaggag gacgttgcgg cccttggggc cgagggtgac cttgacggcc ctggaaatcg
      961 tctcgacgcc gacgaggagc ttgcgcctcg cgtcttcgct gaagaggagc tgtttcgcca
     1021 tagcttggtt ctccctttac ggtaaggttt attccgatgc gccgggcgcc tgggcggggc
     1081 ttggccccgc cggcgtggcg cattccggtt gccggtgact ggtcccggcc attcgcggcc
     1141 ggggtcgtcg tagaagatac aaaaagcgtg gacgagcgac aagggcttcc ggaaaaaatc
     1201 gaaaatggtt actatagtaa ccatatggac gagatcgccc tcatggagtc gatcgcctcg
     1261 agctggcggc ggctctccgc gaagcggatc aggcccttcg agatcagcct cctccagtac
     1321 cgcctcatcc tcctcgccag gcggaagggc tcggtgagcc tctcccaggc ggccgagggc
     1381 ctgggttgcg accgccccgg cttgaccctc ctcgccagga agtgcgtgcg ctccggctgg
     1441 ctccgccgcc ggcgctcggc cgccgaccgg cgctcccacc gcctggagct gaccggcgag
     1501 ggggaggagc tcctcgacgc gatcgaagcc gcccaggctt cgccctcggc ggcagcggcc
     1561 gatcccctcg acgtccttgc gcccgccgag cgggaggcct tggccgggat gctccgccgc
     1621 gtgcgcgacc gcgccgagga gctccgggcc ggtcgctgag ggatgcctag gcagctgccg
     1681 tcggcgtgcc cgtatgcagg atcttcgccc gtccgcaagg ccgattccag cgcgccccta
     1741 aggccgctcg cggcgcggcg cgcgaggctc cgcgtcgcgg cgttgcgaag aaaggctttt
     1801 tactgtatgc tttcgcaaca tgcgcgagac gttcaagatc atctacagcg acggcgacat
     1861 cctcgtcgtc gagaagccgg ccggcctcct ctccgtgccc gaccgttacg agcccgaggt
     1921 gcccgccctc gtcacggagc tccgcaagcg cctcgccgag ggcctcctgc tgcccttggc
     1981 caaggccgag ggcgccgcgg aaggcgcagg cccggcggcc gccgaagcct ccgggcagaa
     2041 agccgccgag acggctcagc cagtcggcca ggccgatccc gcccgcggag ccgagctcct
     2101 cgtcgtgcac cggctcgaca aggatacctc cggcctgctt atattcgcgc ggaacgcgga
     2161 ggcccaccgc cgcctcaacg gcgccttcgg cacgggcgcc gtcaagaagc gctaccgcgc
     2221 catcgtgaag ggctccccga actgggagga gacgacctgc gagctccccc tgcacgtgga
     2281 cgcggacagg agccaccgga cgatcatcga cggccaccac ggcaaggaat cccggacggt
     2341 cttcgccgtc ctggaccgct atcgcggcgc gggcggcttc tcggcggccc tcgtcgaggc
     2401 gaggccggag accggccgca cccaccagat ccgcgtccac ctggcctccc tgggccatgc
     2461 ctgcctctgc gatcccctct acggcgacgg ggaacctctc tacctctcga aggtgaaggg
     2521 caagtggaag ggcgacgagt ggtccgaccg ccccctcctc tcgcggaccg ccctccacgc
     2581 cttctccctc gatttcgacc acccgatgac gggcgagccc ctccatttcg aggcggccga
     2641 gcccaaggac ttccgcgccg cgcgaatcca gctggaaaag ctctagaaaa agccggggcg
     2701 gaggccttgg acgcccccgc ggcgtccccg gcctccgcgc gtccgaggcg cgtccgggcg
     2761 cgcccgcggc gccggcctgc gcgcgtcctg gcctcctccc cggcgcgggg ctgtgcccgg
     2821 cgtcgcttgg ccccgtcctc cgcgctcgtc ctcgcttccg gccgctcacc gattat
//
LOCUS       DAOOSJ010000291         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23526_length_2862_cov_6.602423, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000291 DAOOSJ010000000
VERSION     DAOOSJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23526_length_2862_cov_6.602423"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLG14_11465"
     CDS             complement(<1..184)
                     /locus_tag="PLG14_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOX49795.1"
                     /translation="MKRIASLFVLLVAIVGLAAAQAKSVGAYTTLEEPLAKELFDQFE
                     KETGIKVNWQRLAGGEV"
     gene            731..1177
                     /locus_tag="PLG14_11470"
     CDS             731..1177
                     /locus_tag="PLG14_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOX49796.1"
                     /translation="MKSMTVYDSMFPAFRVDPFEMLEQVFESEPFFAGAVRTPAVDVR
                     EEEGRYLIEAELPGLSEKDLKLELKDGVLELSTAKKEEAKDQSEEKGRRWIRRERRDF
                     AFSRSFALPEDADGERIEARFKDGLLTIELPKKPETAPRLVPVKVA"
     gene            complement(1389..2171)
                     /locus_tag="PLG14_11475"
     CDS             complement(1389..2171)
                     /locus_tag="PLG14_11475"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOX49797.1"
                     /translation="MAERTPILFFGHGSPMNAVEDNGYTRALQALGGRLERPKALLCV
                     SAHWTAPGLRLGAAVAPRLVYDFYGFPPELYRVAWPAPGDPGSARRAAALLAGFEPGL
                     DERRGLDHGVWSSLARVFPEAEVPVFQLSLDPERPASEHLAIAAALAPLRAEGVLVAG
                     SGNVVHNLGEVSFGGEAPPPDWALSFDEAMARALAGRDLGAVVRAVDASREGRLRAHP
                     TVEHLLPLLYALALAAPDEGPETVYEGFQNGSISMRTVGWGL"
     gene            2444..>2862
                     /locus_tag="PLG14_11480"
     CDS             2444..>2862
                     /locus_tag="PLG14_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX49798.1"
                     /translation="MAMREIIEIDEAKCDGCGACETGCPEGALRIIDGKARLVGESLC
                     DGLGACIGNCPRGAIKVTRREAEPYSEAKVIEGIAKLGPKVVEAHLEHLLRHGQDGYA
                     REALAWLEEQGIAAPDSAAAALGKASALGKASALGKAS"
BASE COUNT          492 a          962 c          989 g          419 t
ORIGIN      
        1 caacctcgcc gccggccagg cgctgccagt tgaccttgat cccggtctcc ttctcgaact
       61 ggtcgaagag ctccttggcg agcggctcct ccagggtggt gtaggcgccg acggacttgg
      121 cctgggcggc cgcgaggccg acgatggcga cgagcaggac gaataggctg gctattctct
      181 tcacataagc tcctttcatg cacggcttgg ccgtgtgtta tagcagtgtt agggaattgt
      241 aaaccctttt ggagtttttg caagcctggg cgctaaatcg gtcaagtgac gaggttttta
      301 catatggacg acccggggcg agaacttact ctctaggaag cgccaatcga caaaacaggg
      361 tgtttctttt ggatacacat tactgtttcc ttattgcctg actcgtatcg ataccactca
      421 gggcttcgca cgaggcggcc gctccagacg gcaggaaacg attgaccccg tttcaggtcc
      481 acccggcccg gagacggcca tctcgcgatc ctttgcgcca tgtctttgtg gcgaaacata
      541 ttgcctacaa tggcaagaag gcggcggaag ctcgaatatt ttttctgccc gatgtgccga
      601 ttttggcacg gagcttgcta tataactagc gagcgccgaa aagggctccg tccaagcgcg
      661 aaagccaaga ccgcgtcgcg gtccaaagcg cctggacgaa cgagactacc gccttatgga
      721 gggtaccaat atgaagagca tgaccgtcta tgattcgatg ttccccgctt tccgcgtcga
      781 ccccttcgag atgctcgagc aggtcttcga gagcgagccc tttttcgcgg gcgccgtgag
      841 gacccccgcc gtcgacgtgc gcgaggagga gggccgctac ctcatcgagg ccgagctccc
      901 cggcctctcc gagaaggacc tgaagctgga gctcaaggac ggcgtcctcg agctctcgac
      961 cgcgaagaag gaggaggcca aggaccagtc cgaggagaag ggcaggcgct ggatccgccg
     1021 cgagcgccgc gacttcgcct tcagccgctc cttcgccctt cccgaggacg ccgacggcga
     1081 gaggatcgag gcccgcttca aggacggcct cctcacgatc gagctgccca agaagcccga
     1141 gaccgcgccg cggctcgtcc cggtgaaggt cgcctgacgc ggcccggcgg ccggaatggc
     1201 cgccaagcgc cggcggccgt cctggacggc cccgcgccgc ccgcggtccc gcgcggccgc
     1261 cggcagccgc gcgccgcccg cggccgcgcg cggagcacga gccgccccgt caaagcgaag
     1321 cgccccccgc cggggcgctt ctttttttcc agcgagagac tcgtgggccg cacaagcagg
     1381 gtcggaggct acagccccca cccgaccgtc ctcatggaga tcgatccgtt ctggaaaccc
     1441 tcgtagaccg tctccggccc ctcgtccggc gcggcgaggg caagcgcgta gaggaggggg
     1501 aggagatgct ccacggtcgg atgggcccgg agacggccct cccgggaggc gtccaccgcc
     1561 cggaccaccg ctcccaggtc gcggccggcc aaggcccggg ccatcgcctc gtcgaaggag
     1621 agggcccaat cgggaggcgg cgcctcgccg ccaaaggaga cctcgcccag gttgtggacg
     1681 acgttgcccg agccagccac gaggacgccc tccgcgcgaa ggggggcgag ggcggcggct
     1741 atcgcgaggt gctcggaggc gggccgctcc gggtccaggg agagctggaa gaccggcacc
     1801 tcggcctcgg ggaagacccg ggccagggac gaccagacgc catggtccag gccgcggcgc
     1861 tcgtccaggc cgggctcgaa gccggccagg agcgcggccg ccctccgggc cgagccggga
     1921 tcgcccgggg ctggccaggc cacgcggtag agctcgggag ggaagccgta gaaatcgtag
     1981 acgaggcgcg gagcgacggc ggctccaagc ctgaggccgg gcgccgtcca atgcgccgag
     2041 acgcagagca gggccttcgg cctctcgagc cgcccgccga gggcctggag ggcccgggtg
     2101 tagccgttgt cctccacggc gttcatgggc gagccgtgcc cgaagaagag gatcggcgtc
     2161 cgttccgcca tgcttagcct ccttgcgtcg cgccttgagt atcgtcgagc cgcgccagga
     2221 atgcgagacc ggaccgccgc cccggccgcg gcgcatagcc ttggcctcgg cacgaagcct
     2281 tggcctcgct ccgtaggggc gggctgagtc cgcgtcgccg cctccgcggg cgggccggtc
     2341 gaaaaagcgc tccttcttcc ggattccgta accgatgtta ccgcggcccg cggcgctctt
     2401 ccctatactc gagccagggc acgcggccca ggaggagcga ccaatggcta tgcgcgagat
     2461 catcgagata gacgaggcga agtgcgacgg ctgcggagcc tgcgagacgg gctgcccgga
     2521 aggggccctg cggatcatcg acgggaaggc gcggctggtc ggcgagagcc tctgcgacgg
     2581 cctgggcgcc tgcatcggca actgccccag gggcgcgatc aaggtcacga ggcgcgaggc
     2641 cgagccctac agcgaggcca aggtcatcga gggcatcgcg aagctggggc ccaaggtcgt
     2701 cgaggcccat ctcgagcatc tcctccgcca cggccaggac ggctacgcga gggaggccct
     2761 cgcctggctc gaggagcaag gcatcgcggc tccggactcc gcggcggccg cgctcggaaa
     2821 ggcctccgcg ctcggaaagg cctccgcgct cggaaaggcc tc
//
LOCUS       DAOOSJ010000292         2836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_23864_length_2836_cov_5.725638, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000292 DAOOSJ010000000
VERSION     DAOOSJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23864_length_2836_cov_5.725638"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLG14_11485"
     CDS             <1..629
                     /locus_tag="PLG14_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOX49799.1"
                     /translation="ALTSIVLFAAFVEAVGISAADVAVSSPAVIIGLFIGSMIPFLFS
                     SLAMDAIGVSAFAMIEEVRRQFRERPGILSDDETPDYAACVDISTKSALRRMVLPGVI
                     AVAAPVSVGLAGGPGMLAGLLAGSTATGVVLAIFMANSGGAWDNAKKLIEGRDVEGKG
                     SASHKAAVVGDTVGDPFKDTAGPAINILIKLMAIIALVMAPLLAGRWR"
     gene            633..1418
                     /locus_tag="PLG14_11490"
     CDS             633..1418
                     /locus_tag="PLG14_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49800.1"
                     /translation="MQSDRHLYLILHPNHSLIASQLPPAEFIKHYVQGSTRYFEGRLI
                     FAELDPGFRDPYFDIDGAYADLVPHEDGRPKATKFIQSYRVLEHVDFDALGKLYVCNS
                     VGDFVALDPADYDPKARGDEMRIILEINPVKFIVLTKYNFIEFSKFITDPHNSKGAPK
                     MFYAQLEFSVEDFMREFEENPFIRCYVPGIHPARLRNGILEVRNTPGKSVKGISLDCP
                     IDKISYKLLRHGFMFASQGKNKFYPLLPLEDVERNFYKFWKSM"
     gene            1434..1811
                     /locus_tag="PLG14_11495"
     CDS             1434..1811
                     /locus_tag="PLG14_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOX49801.1"
                     /translation="MAITRISTKAAPAALGPYAQGVRSGGLVFCSGQLGIDPATGALE
                     AGVEAQARRAMENLKAVLAAEGLGFADVAKTTIFLADMADFAKVNEVYGSYFEGAYPA
                     RSTIQVAALPKGGLVEVEAIAVA"
     gene            complement(1836..>2836)
                     /locus_tag="PLG14_11500"
     CDS             complement(1836..>2836)
                     /locus_tag="PLG14_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49802.1"
                     /translation="AAAPGAAALGGATAGGAAAVGGTVAAGVPAAAGGATAAGPLGGA
                     AFRAAGARAASAGGAAAASPFLWAGLAGLFLGLALGEAFHWRRRARRGEEKAAARLSA
                     LAAFCLAVALAAGACVLILPEKASLGDPLLPYWAGLALACGLAAGLAPRAAGLPLLGL
                     VLAAALLLVESLAGWLPLAGPTPVARLLPFSVGPDGFSGELEVLERDSVPTVQRLELK
                     AASAALVVERLELEGPAALVAGPAFYRVVGLSGGPGGDSVSFPARRSLLERVLPLPEG
                     PEAEARGLLVRRSREASAALPLAALEPVLYSLETGGGGEGLALVAAPESLPFGAKD"
BASE COUNT          448 a         1054 c          956 g          378 t
ORIGIN      
        1 ccgccctcac ctccatagtc ctcttcgcgg ccttcgtgga ggccgtgggc atctccgcgg
       61 ccgacgtcgc ggtgagctcg ccggccgtca tcatcggcct cttcatcggc tcgatgatcc
      121 ccttcctctt ctcctccctc gcgatggacg ccatcggcgt ctccgccttc gcgatgatcg
      181 aggaggtgcg gcgccagttc cgcgagcggc ccggcatcct ttccgacgac gagacgcccg
      241 actacgcggc ctgcgtcgac atctcgacga agtcggccct gcgccgcatg gtcctgcccg
      301 gggtcatagc cgtggcggcg cccgtgtccg tcggcctcgc gggcgggccg ggcatgctcg
      361 ccggcctcct cgcgggctcc accgccacgg gcgtggtcct cgcgatcttc atggcgaact
      421 cgggcggcgc ctgggacaac gcgaagaagc tcatcgaggg cagggacgtc gagggcaagg
      481 gctccgcctc gcacaaggcc gcggtcgtcg gcgacacggt cggcgacccc ttcaaggaca
      541 ccgctggacc agcgatcaac attctcatta agctgatggc catcatagcc ctcgtcatgg
      601 cgcccctgct cgcggggcgg tggaggtaac caatgcagtc cgacaggcat ctctatctca
      661 tcctgcaccc gaaccactcc ctcatcgcgt cccagctccc gcctgcggag ttcatcaagc
      721 actacgtcca gggctcgacg cgctacttcg aggggcggct catcttcgcc gagctcgacc
      781 ccggcttccg ggacccctac ttcgacatcg acggcgccta cgccgacctc gtcccccacg
      841 aggacggcag gcccaaggca accaagttca tccagagcta ccgcgtcctg gagcacgtcg
      901 acttcgacgc cctcgggaag ctctacgtct gcaactcggt cggcgacttc gtcgccctcg
      961 accccgccga ctacgacccg aaggcccgcg gcgacgagat gcgcatcatc ctggagatca
     1021 accccgtcaa gttcatcgtc ctcacgaagt acaacttcat cgagttctcc aagttcatca
     1081 cggatccgca caactccaag ggcgcgccca agatgttcta cgcccagctg gagttctccg
     1141 tggaggactt catgcgcgag ttcgaggaga accccttcat ccgctgctac gtgccgggca
     1201 tccaccccgc ccgcctccgg aacggcatcc tcgaggtgcg gaacaccccc gggaagagcg
     1261 tgaagggcat ctccctcgac tgccccatcg acaagatctc ctacaagctc ctccgccacg
     1321 gcttcatgtt cgcgagccag gggaagaaca agttctaccc cctcctcccg ctcgaggacg
     1381 tcgagaggaa tttctacaag ttctggaagt cgatgtagag gaggacgaag gacatggcca
     1441 tcacgagaat cagcaccaag gcggcgccgg cggcgctcgg gccctacgcg cagggggtcc
     1501 gctcgggcgg cctcgtcttc tgctcggggc agctcggcat cgaccccgcc accggcgccc
     1561 tggaggcggg ggtcgaggcc caggccaggc gcgcgatgga gaacctgaag gccgtcctcg
     1621 cggccgaggg cctcggcttc gcggacgtcg cgaagacgac gatcttcctg gccgacatgg
     1681 cggacttcgc caaggtcaac gaggtctacg gctcctactt cgagggcgcc tatcccgccc
     1741 gctcgacgat ccaggtggcc gccctgccca agggcggact cgtggaggtg gaggcgatag
     1801 ccgtcgcctg actcaagggc ccttcaaggg cccggctagt ccttcgctcc gaagggcagg
     1861 ctttcggggg cggcgaccag ggcaaggccc tcgccgcccc cgcccgtttc cagggaatag
     1921 aggacgggct cgagggcggc caggggcagg gcggcgctcg cctcgcggct ccgcctgaca
     1981 aggaggcccc gcgcctccgc ctcgggccct tcgggcaggg gcaggacccg ctcgaggagg
     2041 ctccgcctcg cggggaagga gaccgagtcc ccgcccgggc cgccggagag gccgacgacg
     2101 cggtagaagg ccggccccgc gacgagggcc gccggacctt cgagctcgag gcgctcgacg
     2161 acgagggccg cggaggcggc cttgagctcg agccgctgca ccgtcggcac gctgtccctc
     2221 tccaggacct cgagctcgcc cgagaagccg tccgggccca ccgagaaggg gaggagcctc
     2281 gcgacgggcg tcggccccgc gaggggaagc cagcccgcca gggactccac gaggaggaga
     2341 gccgcggcga gaacgaggcc gaggagggga aggcccgccg cccggggcgc gaggcccgcg
     2401 gcgaggccgc aggccagggc caggccggcc cagtagggaa gtagcgggtc gcccagggaa
     2461 gccttctcgg gaaggatgag tacgcaggcc cccgccgcga gggcgacggc caggcagaag
     2521 gccgccaggg ccgagagcct cgccgccgcc ttctcctctc cgcgccgcgc gcgacgccgc
     2581 caatgaaagg cctcgcccag ggccaagccg aggaagaggc cggcgaggcc ggcccagagg
     2641 aaggggctcg ccgcggcggc tccgcctgcc gaagccgcgc gggcgcctgc ggcccggaac
     2701 gcggctccgc ccagggggcc cgcggcggtt gccccgccgg cggcggccgg cactccggcg
     2761 gcgactgtcc cgccgaccgc ggccgctccc cccgccgtgg cgccgcctaa ggcggcggcc
     2821 ccgggagccg ccgcct
//
LOCUS       DAOOSJ010000293         2800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_24339_length_2800_cov_4.600000, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000293 DAOOSJ010000000
VERSION     DAOOSJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24339_length_2800_cov_4.600000"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLG14_11505"
     CDS             <1..546
                     /locus_tag="PLG14_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX49803.1"
                     /translation="LDLALVAALEKANEAGKRLAAGSGLFRPDFAFRAELGYSGPRLP
                     FVEEGWEDKDDWSLNLSVATSLELFDGGEARAARGKAAAELEKARARSEEARSGIANL
                     LRSGFLDLELARVRLEYDILKLEGHGEKVKGLRAERDAGSGSEAAYLRALLDTLGTVA
                     DGWARLAEYRGALWKLEAALP"
     gene            complement(600..1883)
                     /locus_tag="PLG14_11510"
     CDS             complement(600..1883)
                     /locus_tag="PLG14_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49804.1"
                     /translation="MNKKTAAGFAALSLLVVLAATASVAFRVLQADSAGRKAAKADFE
                     RLSAILRPLSDSRELADPALRASLGAYYKARSTLLLATVYERGAGLRWRIPARSPYLP
                     AAENEKPLPETSYPPRSSLLLASPLPGDPSGRLVLEALYVTLPQSAVFLAFRDAALGL
                     AAYLVLSALVLLPGAAAASRRRRAGAARSAYRDDASGDAAEDPSGAAGAPEEAGLGAS
                     TEEPAEPFSPLEDEDFQIPDLALDEAPPSATAGHEPGVATGLYSPISGLGWESYLADR
                     LDAELARSASFEQDLCLLLVRGEGLAPGTPSYASVAKAVAEFFSFKDLAFERGPEGFA
                     VILPNIDVDHGLRMAEEFHRKLSAILGGGRAPSGRLSFFMGLSSRAGRLVDSGRVIEE
                     SESALAKAVADRDSRIVAFRPDPDKYRLYLASKGC"
     gene            1982..2797
                     /gene="amrB"
                     /locus_tag="PLG14_11515"
     CDS             1982..2797
                     /gene="amrB"
                     /locus_tag="PLG14_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOX49805.1"
                     /translation="MNTRRRALPPGWYPSDERSVGELIEEWNAEPPPLGALAAVAPHA
                     GWAFSGRLAVKAASALRGAETVVVVGGHLPDGYPVLIAPEKSFETPLGALPADLELAE
                     ALSAEMGLEEDRAADNTVEVQLPIIKARFPDAKILWLRAPAGLKAIALGRALKKAADG
                     LKRRVSCLGSTDLTHYGPDYGFSPAGRGAAAEAWVREKSDKGFIDALLAMDPEAVLAR
                     GEAGAACSSGAAAAALSFALASGAGRASLLGYATSLETRSSESFVGYCAVAFY"
BASE COUNT          402 a          932 c         1106 g          360 t
ORIGIN      
        1 ctggacctgg ccctcgtcgc ggccctggag aaggcgaacg aggcgggaaa aaggctcgcg
       61 gcgggctcgg ggcttttccg ccccgacttc gctttccgcg cggagctcgg ctactcgggc
      121 ccgcgccttc ccttcgtcga ggagggctgg gaggacaagg acgactggag cctgaacctg
      181 agcgtcgcga cgagcctcga gctcttcgac gggggcgagg cccgcgcggc ccgcggaaag
      241 gcggccgccg agcttgagaa ggcccgggcc aggtcggagg aggcgcgctc ggggatagcg
      301 aacctcctgc ggtcgggttt cctcgacctg gagctcgccc gcgtccgcct ggagtacgac
      361 atcctgaagc tcgaggggca cggggagaag gtgaaggggc tcagggcgga acgggacgcg
      421 ggctcgggct cggaggccgc ctacctgcga gccctcctcg acactctcgg gacggtcgcg
      481 gacggctggg cccgcctggc ggagtaccgc ggggccctct ggaagctcga ggccgccctg
      541 ccctaggggg ccactcggcg atccccgccg cggccatcct cgtcgcggcg gccggcggct
      601 cagcagccct tggaggcgag gtagaggcgg tacttgtccg ggtcggggcg gaaggcgacg
      661 atgcgcgaat cgcggtcggc tacagccttc gcgagggcgc tctcggactc ctcgatgacg
      721 cggcccgagt ccacgaggcg gcctgcccgc gaggagaggc ccatgaagaa ggagaggcgg
      781 cccgagggcg cgcggccgcc gcccaggatg gcggacagct tcctgtggaa ttcctcggcc
      841 atgcgcaggc cgtggtcgac gtcgatgttg ggcaggatga cggcgaagcc ctcgggcccg
      901 cgctcgaagg ccaggtcctt gaagctgaag aactcggcga cggccttggc cacggaggcg
      961 taggagggcg tgcccggggc caggccctcg ccgcggacta ggaggaggca gaggtcctgc
     1021 tcgaaggagg ccgacctcgc gagctcggcg tccaggcggt cggcgaggta ggactcccag
     1081 cccaggcccg agatgggcga atagagaccc gtcgccacgc ccggctcgtg gccggcggtg
     1141 gcggagggcg gagcctcgtc cagggcgagg tcggggatct ggaagtcctc gtcctcgagg
     1201 ggagagaagg gctcggcggg ctcttccgtc gacgcgccca agccggcttc ctccggcgct
     1261 ccggccgcgc ctgaagggtc ttccgcggca tcgcccgacg catcgtccct gtacgcgctc
     1321 cgggccgcgc ccgcgcgcct ccggcgcgag gccgcggccg ctccgggaag gaggacgagg
     1381 gccgagagga cgagataggc ggcgaggccc aaggccgcgt cgcggaaggc caggaagacg
     1441 gcggactggg gcagggtgac gtagagggcc tcgaggacga ggcgccccga gggatcgccc
     1501 ggcaggggag aggcgaggag gagggacgag cggggagggt agctcgtctc ggggaggggc
     1561 ttctcgttct ccgccgcggg caggtagggt gagcgggcgg gaatgcgcca gcggagcccc
     1621 gcgccccgct cgtagacggt cgcgaggagg agggtcgacc gggccttgta gtaggcgccc
     1681 aggctcgccc tcagggcggg atcggcgagc tccctcgagt cggagagggg gcggaggatc
     1741 gcggagaggc gctcgaaatc cgccttggcg gccttgcggc cggccgagtc cgcctggagg
     1801 acgcggaagg cgacggaggc cgtcgcggcg aggacgacga ggaggctcaa ggcggcgaag
     1861 ccggctgcgg ttttcttgtt catggcgcgc tcacagtata tactgaagta tcggcctccg
     1921 cgccagctag tttcgcatta ttcgtcgcct gcccggggcg gagatccgag ggggaggagc
     1981 cgtgaacacg agacgacggg ccctgccgcc cggctggtat ccttccgacg agcggagcgt
     2041 cggcgagctc atcgaggaat ggaacgccga gccgccgccc ttgggagccc tcgccgcggt
     2101 cgcgccccac gcgggctggg ccttctcggg ccgcctggcc gtgaaggccg cctccgccct
     2161 ccgcggcgcg gagaccgtcg tcgtggtcgg cggccatctc cccgacggct atcccgtcct
     2221 catcgccccc gagaagagct tcgagacgcc cctgggagcc ctccccgccg acctcgagct
     2281 cgcggaggcc ctctcggccg agatggggct cgaggaggac agggccgccg acaacaccgt
     2341 ggaggtccag cttcccatca tcaaggcgcg ctttcccgac gcgaagatcc tctggctcag
     2401 ggcccccgcg ggcctgaagg cgatagccct ggggcgggcc ctcaaaaagg cggcggacgg
     2461 gctcaagcgg cgggtttcct gcctgggctc gaccgacctc acccactacg gcccggacta
     2521 cggcttctct ccggcgggaa gaggcgcggc tgcggaggcc tgggtgcggg agaagagcga
     2581 caagggcttc atcgacgccc tcctggcgat ggatcccgag gccgtcctgg cgcggggcga
     2641 ggcgggcgcg gcctgctcct cgggcgcggc ggcggcggcg ctgtccttcg ccctggcctc
     2701 cggcgcgggg cgcgcgagcc tcttgggcta cgcgacgagc ctggagaccc gctcgagcga
     2761 gagcttcgtc ggctactgcg ccgtggcctt ctactaggcc
//
LOCUS       DAOOSJ010000294         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_24862_length_2763_cov_6.683900, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000294 DAOOSJ010000000
VERSION     DAOOSJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24862_length_2763_cov_6.683900"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /gene="xdh"
                     /locus_tag="PLG14_11520"
     CDS             <1..2229
                     /gene="xdh"
                     /locus_tag="PLG14_11520"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOX49806.1"
                     /translation="RAAAAGGEAAEAAAAGSPLVGASLVRIDAVEKAEGRALFVDDLS
                     APRMLFGAVLRAPHPRARLLSLDAEAARRAPGVAALALAADIPGRRFIGHIEEDWPTL
                     MEVGEESRCVGDAVALVAAESPEEARAALGLIRAEWEVLPPLRSPAEALARGAPAIQP
                     GGNILSRLDFSRGDADSALAAAAAGGGFLAKGRFSTPFTDHAFMEPESALAFPPDDEG
                     VVLVRTGEQNVFDGRRYVALTLGLPEERVRVVSAYVGGGFGGKEDQTVQHHAALLACL
                     AGRPVKLTFSRQESILVHPKRHAMEIEAEAACDAEGRLTAIRGRVVADTGAYASLGGP
                     VLHRAVTHFGGPYACPNVDVEGLAVYTNNPPAGAFRGFGVPQVNFALESLLDELAGAA
                     GLDPWEFRFRNAVRPGGELPNGQLAGPDTAVEECLLAVKEAYYGARREGKAAGLACGL
                     KNTGIGVGHRDVGRCSVRFTEGGLELLSGAACIGQGLATVLVQVAAGTLGVEPGLVSV
                     ALPDTAATPDSGTTTASRQTLLAGEACRQACLQAVAALAAEPGPGAAAAPGQAAQASR
                     AAGGFPYAALAGRIFRGEYEAKTDPFDSAKANPVRHVAYSYACHLAVLGADGRLERFV
                     AAHDSGLVVNPLAFEGQVEGGVAMGLGYALTEDFPLEDCAPTARFGTLGLLRAADMPP
                     VETLVVRKAALSGAGYGAKGIGEISSIPTASAVANAYRALDGKARARLPLEGTPYGRK
                     RR"
     gene            2331..>2763
                     /locus_tag="PLG14_11525"
     CDS             2331..>2763
                     /locus_tag="PLG14_11525"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOX49807.1"
                     /translation="MSRKTIVVALGGNALIAEGTEGTVAEQFENTRKSMAAIVGMIQE
                     GHKVVLTHGNGPQAGVHLIRNEAGSAQVPPATLNVIVADTQGSIGYMLQQCLQNALLK
                     AGVKKDVVTVVTQVEVDPKDPQMVNPNKYVGPFFKAEQLDAC"
BASE COUNT          372 a         1100 c          932 g          359 t
ORIGIN      
        1 cgggcggccg ccgcgggcgg ggaagcggcc gaagcggcgg ccgcgggctc ccccctcgtc
       61 ggagcgagcc tggtccggat cgacgccgtg gagaaggccg aggggcgggc cctcttcgtg
      121 gacgacctca gtgctccccg catgctcttc ggcgccgtcc tccgcgcgcc ccatccgcgg
      181 gcccgcctcc tctccctgga cgccgaggcc gcgcggcggg cgcccggcgt cgcggccctg
      241 gccctggccg cggacattcc cggaaggcgc ttcatcggcc acatcgagga ggactggccg
      301 acactcatgg aggtcggcga ggagtcccgt tgcgtcggcg acgccgtcgc cctcgtcgcg
      361 gcggagagtc ccgaggaagc ccgcgccgca cttggcctca tccgggccga gtgggaggtc
      421 cttccgcccc tccgctcccc cgcggaggcc ctggcccgcg gcgctcccgc catccagccg
      481 ggcggcaaca tcctctcccg gctcgacttc tcgcggggcg acgccgactc ggccctggcc
      541 gccgcggccg ccggcggcgg cttcctcgcg aagggccgct tctccacgcc cttcaccgac
      601 cacgccttca tggagcccga gagcgcgctc gcctttcccc ccgacgacga gggcgtcgtc
      661 ctcgtgcgca ccggggagca gaacgtcttc gacgggaggc gctacgtcgc cctcaccctg
      721 ggccttcccg aggagcgcgt tcgcgtcgtc tccgcctacg tcggcggcgg cttcggcggc
      781 aaggaggacc agacggtcca gcaccacgcc gccctcctcg cctgcctcgc gggcaggcca
      841 gtgaagctca ccttctcgcg ccaggagagc atcctggtcc acccgaagcg ccacgcgatg
      901 gagatcgagg ccgaggccgc ctgcgacgcg gagggccgcc tcaccgcgat ccgcggccgc
      961 gtcgtcgccg acacgggggc ctacgcctcc ctgggcggcc ccgtcctgca ccgggccgtc
     1021 acccacttcg gcgggcccta cgcctgtccc aacgtcgacg tggagggcct ggccgtctac
     1081 acgaacaacc cgcccgccgg cgccttccgc ggcttcggcg tgccccaggt gaatttcgcc
     1141 ctcgaatccc ttctggacga gctcgcgggg gccgctggcc tcgatccctg ggagttccgc
     1201 ttccgcaatg ccgtcaggcc cggcggcgag ctgccgaacg gccagctcgc ggggcccgac
     1261 acggccgtgg aggaatgcct cctggccgtg aaggaggcct actacggagc ccgccgggag
     1321 ggcaaggccg cgggcctcgc ctgcgggctc aagaacacgg gcatcggcgt cgggcatcgc
     1381 gacgtcgggc gctgctccgt ccgcttcacg gagggcggcc tcgagctcct ctccggggcc
     1441 gcctgcatcg gccagggcct cgcgaccgtc ctggtccagg tcgcggccgg gaccctgggc
     1501 gtcgagcccg gcctcgtctc cgtcgccctc cccgacacgg ccgcgacccc ggactcaggc
     1561 accacgacgg cctcgcgcca gaccctcctc gcgggcgagg cctgcaggca ggcctgcctc
     1621 caggcggtgg ccgccctggc cgcggagccg gggccgggcg cggcggccgc cccgggccaa
     1681 gccgcgcagg cttcccgcgc ggcgggcggc tttccctacg ccgccctggc cggcaggatc
     1741 ttccggggcg aatacgaggc caagaccgac cccttcgact ccgccaaggc gaatcccgtc
     1801 cgccacgtcg cctacagcta cgcctgccac ctcgccgtcc tcggggccga cgggaggctc
     1861 gagcgcttcg tcgccgccca tgactccggc ctcgtcgtga atcccctcgc cttcgagggc
     1921 caggtcgagg gcggggtcgc gatgggcctg ggctacgccc tcacggagga cttccccctc
     1981 gaggactgcg cgccgacggc gcgcttcggg accctcggcc tcctccgcgc ggccgacatg
     2041 ccccccgtgg agaccctcgt cgtccgcaag gcggcgctct ccggagccgg ctacggcgcc
     2101 aagggcatcg gcgagatctc ctccataccg acggcctccg cggtcgcgaa cgcttatcgc
     2161 gccctggacg gaaaggcgag ggcgaggctt cccctggagg gcacgccata cgggcggaag
     2221 cgccggtaga gggcgaccgc gcttgcgcga tgggcgatag tcggtctata atcgctcctc
     2281 ccggccgcgt tccacgcacg gcgccgccgg gcctaaaagg agcgtagatc atgagcagga
     2341 aaaccatcgt cgtcgccctc ggcggcaacg ccctcatcgc cgagggcacc gaaggcacgg
     2401 tcgccgaaca gttcgagaac acccgcaaga gcatggccgc catcgtcggg atgatccagg
     2461 agggccacaa ggtcgtcctc acccacggca acggccccca ggccggcgtc cacctcatcc
     2521 gcaacgaggc cggctcggcc caggtcccgc ccgcgaccct caatgtcatc gtcgccgaca
     2581 cccagggctc catcggctac atgctccagc agtgcctcca gaacgccctc ctcaaggccg
     2641 gcgtgaagaa ggacgtcgtc accgtcgtca cccaggtgga ggtggacccc aaggaccccc
     2701 agatggtcaa cccgaacaag tacgtcggcc ccttcttcaa ggccgagcag ctcgacgcct
     2761 gca
//
LOCUS       DAOOSJ010000295         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_25209_length_2740_cov_5.650652, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000295 DAOOSJ010000000
VERSION     DAOOSJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25209_length_2740_cov_5.650652"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLG14_11530"
     CDS             <1..685
                     /locus_tag="PLG14_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49808.1"
                     /translation="RYLATKIFLEAKRPLPEASAAPGAEAASGDASDQDAAGERPAAL
                     AVVGAGHLAGIEAWLLRLEAGEASADLSGLETLPPPSPWAKAAGWAIPALIVGLIVLG
                     FFRSGVQASLALLLRWILLNGSLAALGSALCLAHPLTIALSFVAAPLATLNPFVGVGL
                     FAGLAEAFLRKPRVADFQGLAEDATSLKGFYRNRVTHVLLVFFLSSFGGMAGNFIALP
                     FLASGAFGG"
     gene            820..1083
                     /locus_tag="PLG14_11535"
     CDS             820..1083
                     /locus_tag="PLG14_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49809.1"
                     /translation="MSLGIAIVLGVVGISALGIVSEIVTTAIKSKAKSRGGPPPAELE
                     ELRARVRALEGRLDERDGAVQRLEEELRFVSRMLEDKSGGGRS"
     gene            1114..>1404
                     /locus_tag="PLG14_11540"
     CDS             1114..>1404
                     /locus_tag="PLG14_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49810.1"
                     /translation="MPRRLGLSPRRPAALPPFLWPIRGRVTSPWFFRHRPDSANPLAL
                     EFHDGLDIAAPAGTPGRVGSTGRSTGPHLHFELKAGGTSLPPRVLLAPHDLRR"
     assembly_gap    1405..1504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1505..2068)
                     /locus_tag="PLG14_11545"
     CDS             complement(<1505..2068)
                     /locus_tag="PLG14_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOX49811.1"
                     /translation="MNERAADAAKAADSTANPAPLGLLGFGLTTILLNLHNAGLYPLD
                     SMIMGMGIFVGGLAQVIAGVEEWKKNNTFGATAFTGYGFFWIALVAAWTLPAMGVARK
                     SEPAEMGFFLLLWGVFTLGMFVGTLRLNRALQVVFGTLAALFFLLAARDFTGNETVGL
                     VAGWIGIFCGFSALYACLAQILNEVYGK"
     gene            2245..2736
                     /locus_tag="PLG14_11550"
     CDS             2245..2736
                     /locus_tag="PLG14_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HOX49812.1"
                     /translation="MERCPCGSGREFAACCGPYLSGAAKAPTAEALMRSRYSAYVKGK
                     VDYIVATCAKDESGGIDVEATRRWSEKSSWLGLQVHRSEKGGAADAEGLVEFTATYVL
                     DGLREEHREIARFAKQDGEWIYVEGEVVPRTVVREGPKVGRNDPCPCGSGKKYKKCCG
                     SAS"
BASE COUNT          370 a          992 c          902 g          376 t
ORIGIN      
        1 ccgctacctc gcgaccaaga tcttcctgga ggccaagcgc ccgctcccgg aggcgtccgc
       61 ggcgcccggc gcggaagcgg cctccggaga cgcctcggac caggatgcgg ccggcgagcg
      121 gcccgccgcc ctcgccgtcg tcggcgccgg ccacctcgcg ggcatcgagg cctggctcct
      181 ccgcctggag gcgggcgagg cctccgccga cctgtccggg ctcgagaccc tcccgccccc
      241 ctcgccctgg gccaaggccg cgggctgggc catccccgcc ctcatcgtcg gcctcatcgt
      301 cctcggcttc ttcaggtccg gcgtccaggc gagcctcgcc ctcctcctcc gctggatcct
      361 cctcaacggc tcgctcgcgg ccctcggctc ggccctctgc ctggcccacc ccctcacgat
      421 cgccctttcc ttcgttgcgg cgcccctcgc gaccctgaac cccttcgtcg gcgtcggcct
      481 cttcgccggc ctcgcggagg ccttcctccg gaagccccgc gtcgccgact tccagggcct
      541 cgcggaggac gccaccagcc tcaagggctt ctaccggaac cgcgtcaccc acgtccttct
      601 cgtcttcttc ctctcgagct tcggaggcat ggcgggcaac ttcatcgccc tgcccttcct
      661 cgcctcggga gccttcggcg gctgagccgc gcatcggggc cgcctgcggc ggccgccagc
      721 gccgacgccg cgggcgcgga cgcgatatcg agggcgctcg gcgtaacgcg ccgggcgcct
      781 gacggcggga gcggcgcggc cgcgtcctag gaggttttca tgtcgcttgg aatcgccatc
      841 gtccttggcg tcgtcggcat ctcggccctc ggcatcgtga gcgagatcgt caccacggcg
      901 atcaagagca aggccaagtc gcggggcggc ccgcctccgg ccgagctcga ggagctccgc
      961 gcgagggtcc gcgccctgga gggccgcctc gacgagcgcg acggagcggt ccagaggctg
     1021 gaggaggagc tccgcttcgt ctcccgcatg cttgaggaca agtcgggcgg aggcaggtcc
     1081 taggggaccg gacggcgcgc ggaggctcgg ccattgcctc gccgcctcgg cctttctcct
     1141 cgtcgccctg ccgccctgcc gcccttcctc tggcccattc gcggccgcgt cacctcccct
     1201 tggttcttcc gccaccggcc cgacagcgcc aatcccctcg ccctcgagtt ccacgacggc
     1261 ctcgacatcg ccgctccggc gggcacgccc ggccgcgtcg gctcgaccgg ccgctccacg
     1321 ggaccccacc tccatttcga gctaaaagcc ggcggaacct cccttccgcc gcgcgtcctc
     1381 ctcgcgcccc acgacctccg ccgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnncttgcc gtagacctcg ttgaggatct gggccaggca ggcgtagagg gcggagaagc
     1561 cgcagaagat gccgatccag ccggcgacga ggccgacggt ctcgttgccc gtgaagtcgc
     1621 gggcggcgag gaggaagaag agggccgcga gggtgccgaa gacgacctgg agggcgcggt
     1681 tcaggcggag ggtgccgacg aacatgccga gggtgaagac gccccagagg aggaggaaga
     1741 agcccatctc ggcgggctcg gacttgcggg ccacgcccat cgcggggagg gtccaggcgg
     1801 cgacgagggc gatccagaag aagccgtagc cggtgaaggc ggtggcgccg aaggtgttgt
     1861 tcttcttcca ctcctccacg ccggctatca cctgggcgag gccgcccacg aaaatgccca
     1921 tgcccatgat catcgagtcg agggggtaga gcccggcgtt gtggagattg aggaggatcg
     1981 tcgtgaggcc gaatccgagg agcccgaggg gcgcgggatt ggcggtgctg tcggcggcct
     2041 tcgcggcgtc ggcggcgcgt tcgttcatga ggttctccgg aagtcgaagg gtgtatccgc
     2101 gcggccatcg gcgcggagcc gcaaccctag cacgccggaa gccgccgcgg taaggggagc
     2161 gccccccatg actcgcccgc cccggcgagg gtagaatgcg ggcgaccctc gcgccatcga
     2221 aaccaagcct tgaggagaaa tagcatggaa cgctgtccct gcggctccgg ccgcgaattc
     2281 gccgcctgct gcggccccta cctctccggc gccgccaagg ccccgaccgc cgaagccctg
     2341 atgcggagcc gctactccgc ctacgtcaag ggaaaggtcg actacatcgt cgccacctgc
     2401 gccaaggacg agtccggcgg catcgacgtg gaggccacgc gccgctggag cgagaagtcc
     2461 tcctggctcg gcctccaggt ccaccgctcg gagaagggcg gcgccgccga cgccgagggc
     2521 ctcgtggagt tcaccgcgac ctacgtcctg gacggcctcc gcgaggagca ccgcgagatc
     2581 gcccgcttcg cgaagcagga cggcgagtgg atctacgtcg agggcgaggt cgtcccccgg
     2641 accgtcgtcc gcgagggccc gaaggtcggc cgcaacgatc cctgcccctg cggctccggg
     2701 aagaaataca agaagtgctg cggctcggcc tcctgacggc
//
LOCUS       DAOOSJ010000296         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_25991_length_2685_cov_5.704943, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000296 DAOOSJ010000000
VERSION     DAOOSJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25991_length_2685_cov_5.704943"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1064
                     /locus_tag="PLG14_11555"
     CDS             141..1064
                     /locus_tag="PLG14_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49813.1"
                     /translation="MKVKTPLAIAAKKCLHESLPPEMISRLALEVVPGYDLSSKTGFP
                     ATMPVPAQLAVSHLVDDVIAKGRFLVLAERLAALDREGFMGRAYRITGLREVFRGIFA
                     EGYLWDEETQLFMEDPKTRRTPDWGRLLEGEEHRFSFLRIDIVKNSRLVHIHGETAAR
                     EAYDDLRNIFARVVELRGGRVWRWEGDGALAAFLFGHSTTSAVLAGMALLHELFLYDR
                     LHNSLGEPLRVRAAVHTGPLRYSPEIGDVLKQETVQEVLDAEGHWTPPGALTITPAVA
                     PTLDRVILGRFKPSLDPSSRLLAYEVRIGTP"
     gene            1061..2026
                     /locus_tag="PLG14_11560"
     CDS             1061..2026
                     /locus_tag="PLG14_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX49814.1"
                     /translation="MRIVFFAADPDDRRLGSVLSRARPREVEFEILPLASFSRSYKKK
                     APGALVYLDASGMDEVRLLECGARLGELPGCAWGAFDRGGELPDPAAVFRAGGKDYLG
                     PALMKGGFEPSRVAAALAFAGLGPAPAGAGTESAFPGWTSLNEGDTIDVRFCYAALGA
                     QRELLEQIGEKRLYALRESFNDFLSPWAAEGGGIVWIREAAGSLVLFPPKDEGMNPVL
                     AAFRLILDRAIVGYEVFNLEIPLTFRFAFHAGRTPWRRIGATGTIVAEDVNFVFHLGG
                     KAAQDGSVLVSKAAADSVPACLKDLFVEAGDYEGRSLLASRRFKD"
     gene            2071..2568
                     /locus_tag="PLG14_11565"
     CDS             2071..2568
                     /locus_tag="PLG14_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOX49815.1"
                     /translation="MLRRAFLAPADSGRVQLLRSMVASFVSFALDFLLCAFLVELAGL
                     SYLPAASLSFAAGTLLNYLLAVAWIFRTSRLKDRRLEFLAYFAFAALGLGLNGAFMYL
                     FTGVAGLHYLVSRVLSGALVFAFNFALRKFVLFGQARPAPGVAPEAEPAAAAATELAP
                     GMDAV"
BASE COUNT          376 a         1030 c          825 g          454 t
ORIGIN      
        1 tctctcccga agtgcccctt taaggcgcct tttcccgtaa aaaccccgta aatcaatgcg
       61 agtccgcgct ttacgccgcg gctttcttgc tctactattt tattcatgca aaaagtaagt
      121 gtcggcgggg gaaggcgggc atgaaggtca agacgcccct cgccatagcg gccaagaagt
      181 gcctccacga gtccctcccg cccgagatga tctcgcggct ggccctggag gtcgtgcccg
      241 gctacgacct ctcctccaag acgggcttcc ccgcgaccat gcccgtgccg gcccagctcg
      301 ccgtctccca cctcgtggac gacgtcatcg cgaagggccg cttcctcgtc ctggccgagc
      361 ggctcgccgc cctggaccgc gagggcttca tgggccgcgc ctaccgcatc acggggctcc
      421 gcgaggtctt caggggcatc ttcgccgagg gttacctctg ggacgaggag acccagctct
      481 tcatggagga cccgaagacc cggcggacgc cggactgggg ccgcctcctc gagggcgagg
      541 agcaccgctt ctccttcctc aggatcgaca tcgtcaagaa ctcgcgcctc gtccacatcc
      601 acggcgagac ggcggcgcgg gaggcctacg acgacctcag gaacatcttc gccagggtcg
      661 tcgagctccg cgggggcagg gtctggcgct gggagggcga cggggccctg gccgccttcc
      721 tcttcggcca ttcgacgacg agcgccgtcc tcgcgggcat ggccctcctc cacgagctct
      781 tcctctacga ccggctccac aacagcctgg gcgagcccct cagggtccgc gccgcggttc
      841 acaccgggcc cctgcgctac agcccggaga taggcgacgt cctcaagcag gagaccgtcc
      901 aggaggtgct ggacgccgag gggcactgga cgccgccggg cgccctcacg atcacgccgg
      961 ccgtggcgcc gaccctggac cgcgtcatcc tgggccgctt caagccctcc ctcgacccct
     1021 cctcccgcct cctcgcctat gaagtcagga tagggacgcc atgagaatcg tattcttcgc
     1081 cgccgacccc gacgaccgcc gcctcggctc cgtcctctcc cgggccaggc cccgggaggt
     1141 cgagttcgag atccttcccc tcgcctcctt ctcccggagc tacaagaaga aagcccccgg
     1201 cgccctggtc tacctcgacg cctcaggcat ggacgaggtg cgcctcctcg agtgcggcgc
     1261 ccgcctgggc gagcttccgg gctgcgcctg gggcgccttc gaccgcgggg gcgagctccc
     1321 cgatcccgcc gccgtcttcc gcgcgggcgg gaaggactac ctggggccgg ccctgatgaa
     1381 gggcggcttc gagccctccc gcgtcgccgc cgccctggcc ttcgcgggcc tcgggcccgc
     1441 gccagccggc gccgggaccg agtcggcctt ccccggctgg acctcgctca acgaaggcga
     1501 cacgatcgac gtccgcttct gctacgcggc cctgggcgcc cagcgcgagc tcctcgagca
     1561 gatcggggag aagcgcctct acgccctccg cgagagcttc aacgacttcc tctccccctg
     1621 ggcggcggag ggcggcggca tcgtctggat cagggaggcg gccggcagcc tggtcctctt
     1681 ccctcccaag gacgagggca tgaaccccgt cctggccgcc ttcaggctca tcctcgaccg
     1741 ggccatcgtc ggctacgagg tcttcaacct cgagataccc ctcaccttcc gcttcgcctt
     1801 ccacgcgggc aggacgccct ggcgccgcat cggcgcgacc ggcaccatcg tcgccgagga
     1861 cgtgaacttc gtcttccacc tcggcggcaa ggcggcccag gacggctcgg tcctcgtctc
     1921 caaggccgcc gcggactccg tgccggcctg cctcaaggac ctcttcgtcg aggcggggga
     1981 ctacgagggc cggtccctcc tggcctcgag gcgcttcaag gactagcgcc gaggtaggcg
     2041 aaggccgggg aacgggctat gctagggccc atgcttaggc gcgccttcct cgcccccgcc
     2101 gactcggggc gggtccagct cctccggtcg atggtcgcga gcttcgtctc cttcgccctc
     2161 gatttcctcc tctgcgcctt cctcgtcgag ctcgccggcc tctcctatct gccggcggcc
     2221 tccctgagct tcgcggccgg caccttactc aattacctcc tcgccgtcgc ctggatcttc
     2281 aggacgagca ggctcaagga caggcgcctc gagttcctgg cctatttcgc cttcgccgcc
     2341 ctcggactcg gcctgaacgg cgccttcatg tacctcttca cgggcgtcgc gggcctccac
     2401 tacctcgtct cccgcgtcct ctcaggcgcc cttgtcttcg ccttcaactt cgccctgcgc
     2461 aagttcgtcc tcttcgggca ggcccggccc gcgccaggcg tcgcccccga ggcggagccc
     2521 gcggccgcgg ccgcgacgga gctcgcgccc gggatggacg cagtctagct accggcccga
     2581 accctcattt tgtgcgttga caaatcccga gagtggcaca atgcttgttt gtggtgagcg
     2641 atcactcact cgccaacctg agcccaaggg agcaagcatg aacta
//
LOCUS       DAOOSJ010000297         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_27027_length_2620_cov_4.160234, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000297 DAOOSJ010000000
VERSION     DAOOSJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27027_length_2620_cov_4.160234"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..1754)
                     /locus_tag="PLG14_11570"
     CDS             complement(399..1754)
                     /locus_tag="PLG14_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX49816.1"
                     /translation="MPLRLTDLGKSVLDTEYAVRGPIVARAQELERSGRKIIYCNIGN
                     PQALEQKPLTYLRQVLSLAAWPELMEKSPNAFPADAVATARAFIAGSKFGIGAYSESK
                     GVRFVRDAVAAFISARDGVPADPDAIYLTDGASKGVQTALKLLIAGPRDGIMIPIPQY
                     PLYSATITLYEGRQVHYYLDEANDWKLSRKMLEDSYDGALSAGTRVKAICVINPGNPT
                     GSVIDRANVAMILDFAREKGIAVLADEVYQENVYRPGDRFVSFARVLEEEKIRDVSLF
                     SFHSCSKGFLGECGLRGGYFEIRNVPADVAAQITKLQSVGLCANLPGQLATYVLVSPP
                     KAGDPSRALYETERDAILGALKERAKLLADGLNRIPGVECNAVAGAMYAFPKIAIPSH
                     RTDEEYCMALLEATGICVVPGSGFGQIPGTAHFRTTILPPTERIKDVVRMFAEFHASY
                     R"
     gene            2183..>2620
                     /locus_tag="PLG14_11575"
     CDS             2183..>2620
                     /locus_tag="PLG14_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOX49817.1"
                     /translation="MPNIAIQSGRNRGSFRGLLLAFAAYAAFAVAAVALLGGCSKSAA
                     SESKLSYEFATVKRSSVSSFVSSSGTLSPLSEVSVMAEMSGRVEKVFVDYNDKVAKGQ
                     VLALLNTDLLELQRKEALAEVRKARAAYELQALDERNKAKLFEK"
BASE COUNT          392 a          789 c         1027 g          412 t
ORIGIN      
        1 ggtatggggg gtatcccccc atccagaggg caacacggaa tgagtggggg tagcggaggc
       61 caccgcaggg cgcgcggcgc gcgcccgagg aggccggagc gagggggagc cgcgtggccc
      121 gcggcgccgc gcccgcgagg aagaccgcga cgcgcgacgc gccttgccct ggaagaggcg
      181 acgccggcgg agacggcctc gggggaatga acccgctcgc gctttccgcg cggctgtcgg
      241 gacggcgggt ggcgcgggag gggcggccgg ccttcggaac gaagcggaga agggccggcc
      301 gcctccccgc gccaggaccc gccccgacgg gctttggaga atctgaccgg ggagcgcgcc
      361 gcgcgacggc ctgggccggg cgctcccccc ttcccctcct agcggtagct cgcgtggaac
      421 tccgcgaaca tccggacgac gtccttgatc cgctcggtgg gcggcaggat ggtcgtgcgg
      481 aagtgggcgg tgccggggat ctggccgaag cccgagccgg ggacgacgca gatgccggtc
      541 gcctccagga gggccatgca gtactcctcg tccgtgcggt gcgaggggat cgcgatcttg
      601 gggaaggcgt acatcgcgcc cgcgacggcg ttgcactcga cgccggggat cctgttgagg
      661 ccgtcggcca ggagcttcgc gcgctccttg agggcgccga ggatggcgtc gcgctctgtt
      721 tcgtacaacg cgcgtgaggg gtcgccggcc ttgggggggc tcacgaggac gtaggtggcg
      781 agctggccgg ggaggttggc gcagaggccg acggactgga gcttggtgat ctgggcggcg
      841 acgtcggcgg gcacgttgcg gatctcgaaa tagccgccgc ggaggccgca ctcgcccagg
      901 aatcccttgg agcaggagtg gaagctgaag agggagacgt cgcggatctt ctcctcctcg
      961 aggacgcggg cgaaggagac gaagcggtcg cccgggcggt agacgttctc ctggtagacc
     1021 tcgtcggcca ggacggcgat gcccttctcc cgcgcgaagt cgaggatcat cgcgacgttg
     1081 gcgcggtcga tcacggagcc ggtggggttg cccgggttga tgacgcatat cgccttcacg
     1141 cgggtgccgg cggagagggc gccgtcgtag gagtcctcca gcatcttgcg cgagagcttc
     1201 cagtcgttcg cctcgtccag gtagtagtgg acctgcctgc cctcgtagag ggtgatggtc
     1261 gcggagtaga gggggtactg ggggatgggg atcatgatcc cgtcccgggg cccggcgatg
     1321 aggagcttga gggcggtctg gacgcccttt gaggcgccgt ccgtgaggta gatggcgtcg
     1381 gggtcggcgg ggacgccgtc gcgggcggag atgaaggcgg ccaccgcgtc gcggacgaag
     1441 cggacgccct tggactcgga gtaggcgccg atgccgaact tggacccggc gatgaaggcg
     1501 cgggcggtcg cgacggcgtc ggccgggaag gcgttgggcg acttctccat gagctcgggc
     1561 caggcggcca gggagaggac ctggcggagg taggtgaggg gcttctgctc gagggcctgg
     1621 gggttgccga tgttgcagta gatgatcttg cggccggagc gctcgagttc ctgggcccgg
     1681 gcgacgatgg gcccgcggac ggcgtactcg gtatcgagga ccgacttccc gaggtcggta
     1741 aggcgtaggg gcatggtttt ctccttggcg tgatcccgat gcggaacgga ggggattgta
     1801 caccgggttc cgggtaatcg gcaaggaatg ggcgagtcgc cggttcggcc ggcgaaacgg
     1861 caccgcggcc ggcgatgggg cgccccgcgt cggcgccgag gccttagccg cggttccggg
     1921 cgctgcgcag tttttgcgcg gcgccgcttg ttgcgcaaaa tctgcgcagt ggtcggaggg
     1981 cggccgaggg gccgggcgaa ggcccgtgcc tggatttagg cccgacctgg cggcttcgcg
     2041 gaggcagggc ggctcttgcg gcttttgcct aagttccatt ggcggaagat attgtcattg
     2101 cggccgctcg accgctcaaa taaatcgcag aaaaatcatt gattggcacg aatcctgcca
     2161 tgaggagggt gtggaggaac ttgtgccaaa catcgccatt cagtcaggcc gcaaccgcgg
     2221 ctccttccgg ggcctgcttc tcgccttcgc cgcctacgcc gccttcgccg tcgcggccgt
     2281 cgccctgctc ggcggctgct ccaagtccgc ggcttccgag tccaagctga gctacgaatt
     2341 cgcgacggtg aagcgctcct cggtctcgag cttcgtctcg agctcgggca ccctctcccc
     2401 gctctcggag gtctcggtca tggccgagat gtcggggcgg gtcgagaagg tcttcgtcga
     2461 ctacaacgac aaggtggcga agggccaggt cctggccctt ctcaacaccg acctcctgga
     2521 gctgcagcgc aaggaggccc tggccgaggt caggaaggcc cgcgcggcct acgagctcca
     2581 ggccctggac gagcggaaca aggcgaagct cttcgagaag
//
LOCUS       DAOOSJ010000298         2521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_28690_length_2521_cov_4.481346, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000298 DAOOSJ010000000
VERSION     DAOOSJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28690_length_2521_cov_4.481346"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="PLG14_11580"
     CDS             complement(<1..1618)
                     /locus_tag="PLG14_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOX49818.1"
                     /translation="MFTKKKIIAFLAACAVFLALDLWMFSSLKSSFAKTLGEGQRQLA
                     TAVARSAPADPEAVEAWLETSRASYPDVGLLYVQGIPGVDESTSEFASDEALLGLYRD
                     NLENPVFLQGIEAASYLETELSEGSFRLGQGRVSLLFAPVLNPEGDTATGLVLVALDD
                     AQLRSFAGLANALAAIALAVFALGFGIATFSRDPLTGYAILVLFGIAAVFVAFPLFEA
                     AKLTFLKDGRFSFAVWGEVLGNRQYLGALWGSIKLGCLTATYSTLIGFIFAFAIARTT
                     IRGKKLLTALATMPVISPPFSLTLSIILLFGNNGLITKRLLHLEGFSIYGLGGLSLVQ
                     TISMFPIAFLTLSGVLQAIDSTLEEASLDLNASRWATFKRVTLPLAAPGLLSAWLLVF
                     TNSLADFANPLLLAGSYRVLSVESYIEVTGMNRLANGAALSILLLLPTLTAFFVQRSW
                     VSKKSFVVVTGKPSGRLSDLASPGLRRVLSAAIVLVSAFIVALYGTIVAGCFVKNWGV
                     DYSFTLANIGEALTRGKDALVSTLLLAGIAT"
     gene            complement(1706..>2521)
                     /locus_tag="PLG14_11585"
     CDS             complement(1706..>2521)
                     /locus_tag="PLG14_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX49819.1"
                     /translation="ETRLEAEKANPQASIWVGGVGLNHMSAKKKGLTAAYKSKLAANT
                     PAQYRDPENFWIGLYVGPLGFITNTKVAAEQGLKAPTSWADLLKPEYKGKIRMGNPTV
                     TGTAYNILTTVRYVFGGDEDKTFDYLAKLDQNVDQYTKSGAAGGKSVAIGEIPIAIGY
                     AHDMVKLKVEGAPVAINIPSEGTGYEVASMSLVKGGKDSVNAKKLYDWVLGKTAQDII
                     GKWYVIPLSKLATKNPVAFSLEDVKTVKQDLQWDADNKVRLLERWTKDIGSKR"
BASE COUNT          426 a          702 c          992 g          401 t
ORIGIN      
        1 gcgtcgctat gcccgcgagg agcagcgtgg agacgagggc gtccttcccc cgggtcaggg
       61 cctcgccgat gttcgccagg gtgaagctgt agtcgacgcc ccagttcttg acgaagcagc
      121 cggcgactat cgtgccgtag agggccacga tgaaggccga gacgaggacg atcgcggcgg
      181 aaaggacgcg gcggagtccc ggggaggcga ggtcggagag ccgccccgag ggcttgccgg
      241 tgacgacgac gaaggacttc ttggagaccc aggagcgctg gacgaagaag gccgtcaggg
      301 tcggcaggag gaggaggatg gagagggcgg cgccgttcgc gagcctgttc atgcccgtga
      361 cctcgatgta ggactccacg gagaggaccc tgtaggagcc ggcgaggagg agggggttcg
      421 cgaagtcggc gagggagttc gtgaatacga ggagccaggc cgagaggagg ccgggcgccg
      481 cgaggggcag ggtcacccgc ttgaaggtcg cccaacgcga ggcgttgagg tcgagggagg
      541 cctcctccag ggtcgagtcg atcgcctgga gcacgccgga gagggtgagg aaggctatcg
      601 ggaacatgga tatcgtctgg acgagcgaga gcccgccgag gccgtagatc gagaagccct
      661 ccaggtggag gaggcgcttc gtgatgaggc cgttgttgcc gaagaggagg atgatcgaga
      721 gggtcagcga gaagggcggc gagatgaccg gcatcgtcgc gagggcggtg aggagcttct
      781 tgcccctgat cgtcgtccgc gcgatcgcga aggcgaagat gaagccgatc agcgtggagt
      841 aggtcgcggt gaggcagccg agcttgatcg agccccagag ggcgcccagg tactgcctgt
      901 tgccgaggac ctctccccag acggcgaagg agaagcggcc gtccttgagg aaggtgagct
      961 tcgcggcctc gaagaggggg aaggccacga agacggcggc tatcccgaag aggaccagga
     1021 tcgcgtagcc cgtgaggggg tcgcgggaga aggtggcgat gccgaagccg agggcgaaga
     1081 cggccagggc tatagccgcc agggcgttcg cgaggcccgc gaaggagcgc agctgcgcgt
     1141 cgtcgagggc gacgaggacg aggccggtcg ccgtgtcgcc ctcgggattg aggacggggg
     1201 cgaagaggag ggagaccctg ccctgcccca ggcggaagct cccctcggag agctcggtct
     1261 ccaggtagga ggcggcctcg atgccctgga ggaagacggg gttctccagg ttgtcgcggt
     1321 agaggccgag gagggcctcg tcgctcgcga actcgctcgt cgactcgtcg acgcccggga
     1381 tgccctggac gtagaggagg ccgacgtcgg gatagctcgc cctggaagtc tcgagccagg
     1441 cctcgacggc ctcgggatca gcgggagcgg agcgcgccac cgcggtcgcg agctggcgct
     1501 ggccctcccc cagggtcttg gcgaagctgg acttgaggga ggagaacatc cacaggtcca
     1561 gggccaggaa cacggcgcag gccgcgagga aggcgatgat cttcttcttc gtgaacacgg
     1621 ggacctactt tgggaagaat ggccgcggcg gcgcgggttc ccccgcgccg ccgcggcgtt
     1681 tcaaggcatc agggcccggg agcggctagc gcttggagcc gatgtccttc gtccagcgct
     1741 cgaggaggcg caccttgttg tcggcgtccc actggagatc ctgcttgacg gtcttgacgt
     1801 cctcgaggct gaaggcgacg gggttcttgg tcgcgagctt cgagaggggg atgacgtacc
     1861 acttgccgat gatgtcctgg gcggtcttgc cgagcaccca gtcgtagagc ttcttggcgt
     1921 tgaccgagtc cttgccgccc ttcacgaggg acatggaggc gacctcgtag cccgtgccct
     1981 cggaggggat gttgatggcg acgggagcgc cttcgacctt gagcttcacc atgtcgtggg
     2041 cgtagccgat ggcgatggga atctcgccga tcgcgacgct cttgccgccg gcggcgccgg
     2101 acttcgtgta ctggtcgacg ttctggtcga gcttcgcgag gtagtcgaag gtcttgtcct
     2161 cgtcgccgcc gaagacgtag cggacggtcg tgaggatgtt gtaggcggtg cccgtcacgg
     2221 tcggattgcc catgcggatc ttgcccttgt attcgggctt gaggaggtcg gcccaggagg
     2281 tcggggcctt gaggccctgc tccgcggcga ccttggtgtt cgtgatgaag ccgagggggc
     2341 cgacgtagag gccgatccag aaattctcgg ggtcgcggta ctgggcgggc gtgttcgccg
     2401 cgagcttgga cttgtaggcg gcggtgaggc ctttcttctt ggcgctcatg tggttgaggc
     2461 cgacgccgcc gacccagatg gaggcctggg gattggcctt ctcggcctcg aggcgggtct
     2521 c
//
LOCUS       DAOOSJ010000299         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_32657_length_2327_cov_5.716989, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000299 DAOOSJ010000000
VERSION     DAOOSJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32657_length_2327_cov_5.716989"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..609)
                     /locus_tag="PLG14_11590"
     CDS             complement(55..609)
                     /locus_tag="PLG14_11590"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOX49820.1"
                     /translation="MRGLAPIAPEYARVLVLGSFPSAASLELGEYYGFARNHFWALLA
                     LAFGEGEPGSWAGKLDLLKRRGVALWDVIAACDREGSLDGAIRNEEANDVLGLLADKP
                     RVVRVALNGGKAAASFRKLLAPELPAGLAIGETVVWRPAALAPRAFSVCRLPSTSPIP
                     TRNFRGPGDKAGLWMAFLSPEVPL"
     gene            874..>2327
                     /locus_tag="PLG14_11595"
     CDS             874..>2327
                     /locus_tag="PLG14_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOX49821.1"
                     /translation="MKSLGINVGSSSLKLVLLDGDKAEWSRVIPHEGDFPGAVAKALS
                     GGAVPPGTKTLVTGNEGRELFAAARTIEPLCVEAALRASALAVDAVVSMGGEDLVVYA
                     VDAGSAIKGSFSGNKCASGTGEFFKQQLARMDMGLEDVAGVSQDAKALPLSMRCSVFM
                     KSDCTHRLNKREATKDDIVLSLSKVMAMKVVDFLKRARIEEGRVALTGGTTRNPHILR
                     YIRELAPGVEFLVPPLADCFEAYGAAVLARESGSPLPAPERLLSGDAIRFGSLPPLEE
                     SRGLVTYFVGRTGPVVAGREYVLGVDGGSTTTKACLVDLETDEVVASHYGRTHGDPVR
                     ALKLCLAELRKKVEADTGGGAISIPYAAATGSSREILGVFLETRGVYNEIIAHSVGTT
                     HFSKDVDTIFEIGGQDAKYVLLKNGVPIDYAMNEACSAGTGSFLEESASGDLSIRNAA
                     EIGPVAVRARRPLKFGEHCSAFINSDIRKAVQQGA"
BASE COUNT          350 a          822 c          814 g          341 t
ORIGIN      
        1 ggcgtaaagc gcggactcgc atcgatttac ggggtttttg cgggaaaagg cgccttaaag
       61 gggcacttcg ggagagagga aggccatcca gagaccggcc ttgtccccgg gccccctgaa
      121 attcctggtc gggatggggc tcgtcgaggg aaggcggcag acggagaagg cgcggggcgc
      181 gagggcggcc ggccgccaga ccacggtttc gccgatggcg aggcccgccg ggagctcggg
      241 cgcgaggagc ttgcggaagg aggcggcggc cttgccgccg ttgagggcca cgcggactac
      301 gcggggcttg tccgccagga ggccgaggac gtcgttggcc tcctcgttcc tgatggctcc
      361 gtccaggctg ccttcgcggt cgcaggcggc gatgacgtcc cagagggcca cgccccgccg
      421 ctttaggagg tctagcttcc cggcccagga tccaggctct ccctcgccga aggccagggc
      481 gaggagggcc cagaagtggt tcctggcgaa gccgtagtat tcgcccagct caagggaggc
      541 ggcggagggg aaggagccga ggacgaggac gcgggcgtat tccggcgcga tgggggcgag
      601 gcctcgcatg ggcaagggga tagcacgggg cggggagggg gcgcaaggcg gggcttcgga
      661 cgcggcggct ctggagcggc ctccgccgcg gcgtcgcttg cagcgtcgct tgcagcgtcc
      721 ccgacggcgc ctcgaggccg ccctttacag cggcgtccag ggagggtagg gttcactttc
      781 cgcgttttcg ccgcgtcgcg gcgcgccggg atgggattcc ttacgcggcc ccttggccca
      841 cttccccggg actccgggta tccaggacgg ttcatgaaaa gcctcggcat caacgtcggc
      901 tcctccagcc tgaagctcgt cctcctggac ggggacaagg ccgaatggag ccgggtcata
      961 ccccacgagg gcgattttcc cggcgcggtc gcgaaggccc tctccggcgg cgccgtgccg
     1021 cccggcacga agaccctcgt cacgggcaac gaagggcggg agctcttcgc cgcggcccgc
     1081 acgatcgagc ccctctgcgt cgaggccgcc ctgcgggcct ccgccctcgc ggtggacgcc
     1141 gtcgtctcca tgggcggcga ggacctcgtc gtctacgcgg tggacgccgg aagcgccatc
     1201 aagggcagct tctctggcaa caagtgcgcc tcgggcacgg gcgaattctt caagcagcag
     1261 ctcgcccgca tggacatggg cctcgaggac gtggccggcg tctctcagga cgccaaggcc
     1321 ctgcccctct cgatgcgctg ctcggtcttc atgaagagcg actgcacgca ccggctcaac
     1381 aagcgcgagg cgacgaagga cgacatcgtc ctctccctct ccaaggtgat ggcgatgaag
     1441 gtcgtcgact tcctgaagcg cgccaggatc gaggagggcc gcgtcgccct caccggcggg
     1501 acgacgagga atccgcacat cctccgctac atccgcgagc tcgcccccgg cgtcgagttc
     1561 ctggtgccgc ccctggccga ctgcttcgag gcctacggcg cggccgtcct cgcgcgggag
     1621 tcgggctcgc ccctcccggc gcccgagcgc ctcctttccg gcgacgcgat ccgcttcggc
     1681 tccctgccgc ccctcgagga gtcgaggggc ctggtcacct atttcgtggg caggaccggc
     1741 ccagtcgtcg cgggccggga gtacgtcctg ggcgtcgacg gcggctcgac cacgaccaag
     1801 gcctgcctcg tcgacctgga gaccgacgag gtcgtcgcct cccactacgg ccgcacccac
     1861 ggcgaccccg tgcgcgccct caagctctgc ctcgccgagc tccgcaagaa ggtggaggcc
     1921 gacacgggcg gcggcgcgat ctccatcccc tacgccgccg cgacgggctc ctcccgcgag
     1981 atcctcggcg tcttcctgga gacccgcggc gtctacaacg agatcatcgc ccattccgtg
     2041 ggcacgaccc acttctccaa ggacgtcgac accatcttcg agatcggcgg ccaggacgcg
     2101 aagtacgtcc tcctcaagaa cggcgtcccc atcgactacg cgatgaacga ggcctgctcc
     2161 gcgggcacgg gctccttcct cgaggagtcc gcctcgggcg acctgagcat ccggaacgcc
     2221 gcggagatcg gccccgtcgc cgtccgcgcc aggcgccccc tcaagttcgg tgagcactgc
     2281 tcggccttca tcaactcgga catccgcaag gccgtccagc agggcgc
//
LOCUS       DAOOSJ010000300         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_32794_length_2321_cov_4.639894, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000300 DAOOSJ010000000
VERSION     DAOOSJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32794_length_2321_cov_4.639894"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1180
                     /locus_tag="PLG14_11600"
     CDS             617..1180
                     /locus_tag="PLG14_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOX49822.1"
                     /translation="MPDPRCPACGKALVSERGLCMRCRKRSWSFDEASSLFAYEGKAA
                     ALILAYKIGGRRSLAAFLAGLLAREIEARHPGRILVPVPPRPEKLRERGWDQVEELVR
                     RLERLGFRAARLLARLPSAQQKRLGLEDRFENARAAYRLLPGAKAAGRLLLVDDVLTT
                     GATAEACSLALREGGAESVAFVSLASD"
     gene            1300..1749
                     /locus_tag="PLG14_11605"
     CDS             1300..1749
                     /locus_tag="PLG14_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOX49823.1"
                     /translation="MQEQETIIADVEPLLKAAGISLVELSLSRGRGEFRARAVVYAPA
                     GTGTAECAKAHRIIYPRLQVLLKTEDPFLEVASPGIDRALKSARECALFVGKGVKLLL
                     EHESEWIRGRLDAVDGRKLSLTTKEGPVTIEFDAVAKARLDSTQEGD"
     gene            1767..>2321
                     /locus_tag="PLG14_11610"
     CDS             1767..>2321
                     /locus_tag="PLG14_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HOX49824.1"
                     /translation="MAEAIRQLINEKGISEDLVVKTVEEALLAAYKRKYGTNENAVVR
                     FKDDYEGVDIYAKKTIVEEVDDPVLEISLEEAQEMADGAEVGDILELPVDPHEFDLQS
                     VMLAKQTTRQNLREISRDTLYAEYKSKVGEIIIGYYQRERNGNIFVDLGKMEGIFPKK
                     YQSPRENYHMGDRIKAMIVEVEKGR"
BASE COUNT          372 a          843 c          778 g          328 t
ORIGIN      
        1 tcttctcgcc gggcgacctc gtcctcgcgg agctcgcctc cctcgacacc gactccctca
       61 ccccccttga ggccctcacc cgcctcgcca tcctcaagaa gcggcttcaa gcgggctgag
      121 cagccgcggg cgtggggttc tcatgggaac ccccgcgctc gcgcgccgcg gggccgcgcc
      181 gccgcgccgc aaggccgcgc cgccgcgcct cgggccgccg actccccgcc tcgggtcgcc
      241 aggcctcggg ccgctgccgc caggcctcgt gccgccaggc ctcgggccgt cgcctaagga
      301 ggcggccggc cggggccggt ttatcctccg cggcctcggc gacctgtcct gcctcttgtt
      361 gcctgtccgc tgcgtcctct gcggagccgc cctgggcttc ggcgggtccg cgtccgaagg
      421 cgggccagaa acgggcgggc ccgcaacgga cggacctgca acgggcggac cggccggagc
      481 gggcgggccg gcgggggagg gcgggccagg cagcgagccc ggcacggccc gcgagcgcgt
      541 ccgagcgggg gagggcaggg ccgcgtgccc gctcttgccc ccgatctgcc cggactgcgc
      601 cgcctccttg cggcccgtgc ccgatccgcg ctgtcccgcc tgcggcaagg ccctcgtgtc
      661 ggagcggggg ctctgcatgc ggtgccggaa gcggagctgg tccttcgacg aggcgagctc
      721 gctcttcgcc tacgagggga aggccgcggc cctcatcctc gcctacaaga tcggcggcag
      781 gcgctccctc gcggccttcc tggccggcct ccttgcccgc gagatcgagg cgcgccatcc
      841 cggacggatc ctcgtgccgg tccctccgcg gccggagaag ctccgcgagc ggggctggga
      901 ccaggtcgag gagctcgtcc gccgtctgga gcggctgggc ttccgcgcgg cgaggctcct
      961 cgcccgcctc ccctcggccc agcagaagcg gctcggcctc gaggaccgct tcgaaaacgc
     1021 gagggccgcc taccgcctcc tccccggggc caaggcggcc gggcggctcc tcctcgtcga
     1081 cgacgtcctc acgacggggg cgacggcgga ggcctgctcg ctggccctca gggaaggcgg
     1141 agccgagtcc gtcgccttcg tctccctggc ctccgactag gggccgcccc tcggcttgac
     1201 gagggggacg aaaacgagta gtatccgcag tatgtaaaaa agaaaagatt ctccttagga
     1261 ataagaagag tcgtcgaggc gactcttttt tttataggta tgcaagaaca agaaacgatc
     1321 atcgccgacg tcgagccgct tctcaaggcc gccggaatct cgctggtgga actgtcccta
     1381 agccgcggcc gcggcgagtt ccgcgcgagg gccgtcgtct acgccccggc cggcaccggc
     1441 accgccgagt gcgcgaaggc ccaccgcatc atctaccccc ggctgcaggt cctcctcaag
     1501 acggaggatc ccttcctcga ggtcgcgagc cccggcatcg accgggccct gaagtcggcc
     1561 cgcgagtgcg cgctcttcgt cgggaagggc gtgaagctcc tcctcgagca cgagagcgag
     1621 tggatccgcg gccgcctgga cgcggtcgac ggccggaagc tctccctcac gacgaaggag
     1681 gggccggtca ccatcgaatt cgacgccgtc gccaaggctc ggcttgactc tactcaggag
     1741 ggagactaag tcctatggct tccgaaatgg ccgaagcgat acgccagctc atcaacgaga
     1801 agggcatctc cgaagatctc gtcgtcaaga ccgtcgagga ggccctcctc gccgcctaca
     1861 agcggaagta cggcaccaac gagaacgccg tcgtccgctt caaggacgac tacgagggcg
     1921 tggacatcta cgccaagaag accatcgtcg aggaagtcga cgatcccgtc ctggagatct
     1981 ccctcgagga ggcccaggag atggcggacg gcgccgaggt cggcgacatc ctcgagctcc
     2041 ccgtcgaccc gcacgagttc gacctccagt cggtcatgct cgcgaagcag acgaccaggc
     2101 agaacctccg cgagatctcc cgcgacaccc tctacgccga gtacaagtcc aaggtcggcg
     2161 agatcatcat cggctactac cagcgcgagc ggaacggcaa catcttcgtg gacctgggca
     2221 agatggaggg catcttcccg aagaagtacc agtccccccg cgagaactac cacatgggcg
     2281 accgcatcaa ggcgatgatc gtcgaggtgg agaagggccg c
//
LOCUS       DAOOSJ010000301         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_33288_length_2301_cov_3.159840, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000301 DAOOSJ010000000
VERSION     DAOOSJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33288_length_2301_cov_3.159840"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1192
                     /locus_tag="PLG14_11615"
     CDS             152..1192
                     /locus_tag="PLG14_11615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR00034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOX49825.1"
                     /translation="MISPDGLARELPLPPRAAETVQAGRKAVASVLRGEDGRLLLLVG
                     PCSLHDRGAALDYGQRLASLGARVEGKILLVMRAYVEKSRTGLGWRGLAEEPSIVGGR
                     DPEAGAAAARSLLVELASLGLPLGTEIVSPYLWRYWEDCLSWAALGARGVEAQALRET
                     AAALPFPCAFKNSLSGGADSAVQAALVASRPCSFLGLGRDGQAAAIEAEGNPLPHLVL
                     RGGRPRRELRGWGPGREPRPNYLASRRALRGMAEAGLKPALLIDASHDNSRPFGQAFA
                     ARAAFRLALRGRKRGGGLAGIRGLMLESNLESGCQAPGAPAALRYGVSITDPCLGWAE
                     TEALILEMAGRL"
     gene            complement(1324..>2301)
                     /gene="gnd"
                     /locus_tag="PLG14_11620"
     CDS             complement(1324..>2301)
                     /gene="gnd"
                     /locus_tag="PLG14_11620"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR00873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOX49826.1"
                     /translation="DPEAWPLLKPLLEAIAAKTPAGEACATWMGPGGSGHFVKMTHNA
                     IEYADMELIAETYHLLRGYLKTSHDEMRTVFAEWNKTELSSYLVGITADVLGVMDADG
                     EPLVEKILDAAGQKGTGAWAAAAALDLGAPVGLLAEAVFARDLSAFKDERVGASAVLS
                     GPKAAPTGERHSMIEDLRKALLAAKIVAYAQGFLLLRLASEEHGWKLDLAAVALVWRE
                     GCIIRSAFLDRIAEAYRRDPGQASLLLDTYFKALLDQSMPSLRKVTAKAIESGTPVPA
                     FAAAVSFYDGYRSTWLPANLLQALRDRFGAHGYERIDRPRGERFHSDWK"
BASE COUNT          293 a          855 c          811 g          342 t
ORIGIN      
        1 acgttcaaat cggtaagttg ctcgtactcg atgcgagatc gtaccacgga tgcccaaaaa
       61 aaagcaagag ggaaaacgcg tttttttcgc ggctctccaa tggccgccca aggtgactcg
      121 aaagcggcct tgaggctata ctcggtccgc catgatctca cccgacggac tcgcccgaga
      181 gcttcccctc cccccccgcg ccgcagagac ggtccaggcg ggccggaagg ccgtagcctc
      241 ggtgctccgg ggcgaggacg ggcgcctcct cctcctcgtc ggcccctgct ccctccatga
      301 ccgcggggcc gccctggact acggacagag gctcgcgtcc ctgggagcca gggtcgaggg
      361 caagatcctc ctcgtcatgc gcgcctacgt cgagaagtcc aggacgggcc tgggctggag
      421 gggcctcgcc gaggagccct ccatcgtcgg gggcagggat cccgaggccg gggcggccgc
      481 cgcgaggagc ctcctcgtcg agctcgcctc cctcggcctt cccctgggca cggagatcgt
      541 ctccccctat ctctggcgct actgggagga ctgcctctcc tgggccgccc tgggcgcccg
      601 cggcgtggag gcccaggccc tccgcgagac cgccgccgcc ctccccttcc cctgcgcctt
      661 caagaactcc ctctcgggcg gcgccgactc ggcggtccag gccgccctgg tcgcctcccg
      721 gccctgctcc ttcctggggc tcggccgcga cggccaggcc gccgcgatcg aggcggaggg
      781 caatcccctg ccccacctcg tcctccgcgg cggccgtcct cgccgcgagc tcaggggctg
      841 gggcccgggc cgggagccga ggcccaacta cctggcctcc cgccgcgccc tccgcggcat
      901 ggcggaggcc ggccttaagc ccgccctcct catcgacgcc tcccacgaca acagccggcc
      961 cttcggccag gccttcgcgg cgcgggccgc cttccgcctg gccctgcggg gcaggaagcg
     1021 cggcggcggg cttgcgggca tccggggcct catgctcgag tcgaacctgg aaagcggctg
     1081 ccaggctccc ggcgcgcctg cggccctccg ctacggcgtc tccatcaccg atccctgcct
     1141 gggctgggcc gagaccgagg cgctcatcct cgagatggcc gggcgcctct agccgggcgc
     1201 tacgggccgg gtccggccgc caaggcccgg cggccggcca aggcccggcg cggcctgtct
     1261 ccgcgcccgc ccgggcggcg cggcgccgcc ggggctggat agggccggtc cagccgatcc
     1321 tcgctacttc cagtcgctgt ggaagcgctc tccccgcggg cggtctatcc tctcgtagcc
     1381 gtgggcgccg aagcggtcgc gcagggcctg gaggaggttc gcgggcagcc aggtgctccg
     1441 gtagccgtcg tagaaggaga cggccgccgc gaaggccggt accggcgtcc cggactcgat
     1501 ggccttggcg gtcaccttcc tcaaggaggg catggattgg tccaggaggg ccttgaagta
     1561 ggtgtcgagg aggaggctcg cctggcccgg gtcccgcctg taggcctcgg cgatgcggtc
     1621 caggaaggcc gagcggatga tgcagccttc ccgccagacc agcgcgaccg cggccaggtc
     1681 gagcttccag ccgtgctcct cggaggcgag cctgaggagg aggaagccct gggcgtaggc
     1741 gacgatcttc gccgcgagga gggccttgcg caggtcctcg atcatcgagt gccgctcgcc
     1801 cgtcggcgcc gccttcgggc cgcttaagac cgcgctcgcg ccgaccctct cgtccttgaa
     1861 ggccgaaagg tcgcgggcga agacggcctc ggcgaggagg ccgacgggcg ccccgaggtc
     1921 gagggccgcc gccgccgccc aggcgcccgt gcccttctgg ccggccgcgt cgaggatctt
     1981 ctccacgagg ggttcgccgt ccgcgtccat caccccgagg acgtccgccg tgatgccgac
     2041 gaggtagctc gagagctccg tcttgttcca ttccgcgaag accgtccgca tctcgtcgtg
     2101 gctcgtcttg aggtagccgc ggaggaggtg gtaggtctcc gcgatgagct ccatgtcggc
     2161 gtactcgatg gcgttgtgcg tcatcttgac gaagtggcct gagccgcccg ggcccatcca
     2221 ggtcgcgcag gcctctcccg cgggcgtctt ggccgcgatg gcctcgagga ggggcttgag
     2281 gaggggccag gcctccggat c
//
LOCUS       DAOOSJ010000302         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_33442_length_2294_cov_4.215275, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000302 DAOOSJ010000000
VERSION     DAOOSJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33442_length_2294_cov_4.215275"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="lepB"
                     /locus_tag="PLG14_11625"
     CDS             <1..688
                     /gene="lepB"
                     /locus_tag="PLG14_11625"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOX49827.1"
                     /translation="RLLSRLKAFVLILLAFEIVTGLFAAAFALSSSAMSPTFLPGDRV
                     LATPAAFGPRTAFGKLPGPAKPERGDLVLVDPPFAERPSFWASLADSALRFVTFQRVS
                     LLGRGAAAAETGPFLERVIALPGDAIRMDSFVFQVRTAGSEHFLTEFELSKSRYDIEK
                     PDLPPGWLPAYPLSGTMAERTLGKDEYFLACDDRGAEADSRLWGPLGRDRFRAKVFLR
                     YWPLARFGTP"
     gene            772..2181
                     /locus_tag="PLG14_11630"
     CDS             772..2181
                     /locus_tag="PLG14_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX49828.1"
                     /translation="MPFCRSKCAYCDFHSGPLSAAPEGLAARIVEASLERIDALAARF
                     GSAQGPAAGAASAPASVPPSVPPSVQAPGLSPGAGPSRAAGPARGPASPQSAASFRGA
                     GRGSRFDTVYVGGGTPTVLPRPLLARLLEGLAERAGSPREWTVEANPESLDAEALAIF
                     RGAGATRISLGVQSLDDGLLRLLGRPADAAACESALSLAASSGLAVSADLMAGLPRRT
                     RLRDEAARLLDLGAGHLSVYDLVLEEGTPLEARYRGGELELPGEDEAADERGELEDFL
                     ASRGFRRYEVSNYAPPGAESLHNLAYWRMDSYLGVGPGAVSTLQAAGPPGASLRIEEE
                     PSLEAYAMGDPAARGIGRSGTAAPGGRPAMGLETAIPPREAAFEAVMMGFRTVFGVDA
                     RAFEARFGLALEAFVGRSLEAWAGRLAPAAPWPEGSPVASPRPSGELALDGRGLDLLN
                     RFLSDCLGEIEGPSPSSAF"
BASE COUNT          246 a          930 c          766 g          352 t
ORIGIN      
        1 ccgcctcctc tcccggctca aggccttcgt cctcatcctc ctcgccttcg agatcgtcac
       61 gggcctcttc gcggccgcct tcgccctctc ctcctccgcc atgtcgccga ccttcctccc
      121 cggggaccgc gtcctcgcga ccccggccgc cttcgggccc cgcacggcct tcggcaagct
      181 gcccggcccg gcgaagcccg agcgggggga cctggtcctc gtcgacccac ccttcgctga
      241 gcgcccctcc ttttgggcct ccctcgccga ctcggccctc cgcttcgtca ccttccagcg
      301 ggtctcgctc ctgggccgcg gcgccgcggc ggcggagacg gggcccttcc tcgagcgggt
      361 catcgccctg ccgggcgacg cgatccgcat ggactccttc gtgttccagg tcaggacggc
      421 gggcagcgag cacttcctca ccgaattcga gctctcgaag agccgctacg acatcgaaaa
      481 gcccgacctg cccccgggct ggctccccgc ctatcccctc tcggggacga tggcggagcg
      541 gaccctcggc aaggatgagt acttcctggc ctgcgacgac cgcggcgccg aggcggactc
      601 ccggctctgg gggcccttgg gccgcgaccg cttccgtgcc aaggtcttcc tccgctactg
      661 gcccttggcc cgcttcggca cgccttgagc ccccgaggcg cggattccgg aggcccagcg
      721 gcctccggga cggctcccgc ctgttcgccc ccccgttccc tctacgtcca cgtgcccttc
      781 tgccgctcca agtgcgccta ttgcgacttc cattcgggtc ccctttcggc cgctcccgaa
      841 gggctcgccg cgcggatagt cgaggcgagc cttgagcgga tcgacgcctt ggccgcgcgc
      901 ttcggctcgg cccagggacc cgccgccggg gcggcttccg cgccggcctc cgtgccgccc
      961 tcagtgccgc cctcagtgca ggcccccgga ctgtcgcccg gcgcgggccc gagccgggcg
     1021 gcggggccgg cacgaggacc ggcttccccg cagtcggcgg cctcgttccg aggcgcgggc
     1081 cgcggttccc gcttcgacac ggtctacgtg ggcggcggca cgccgacagt cctgccccgg
     1141 cccctcctcg cgcgcctcct cgagggcctg gcggagcgcg cgggctctcc ccgggaatgg
     1201 acggtggagg ccaaccccga gagcctcgac gccgaggcgc tggccatctt ccgcggcgcg
     1261 ggcgcgacgc ggatctctct cggggtacag agcctcgacg acggattgct gcgcctcctc
     1321 gggaggcccg cggacgcggc cgcctgcgaa tcggccctca gcctcgcggc ctcctcgggc
     1381 ctcgccgtct ccgccgacct catggccggc cttccacgga ggacgaggct ccgcgacgag
     1441 gcggcgaggc tcctcgacct cggggcgggg cacctctcgg tctacgacct cgtcctcgag
     1501 gagggcaccc ctctggaagc ccgctaccgc ggcggggagc tcgagctgcc gggagaggac
     1561 gaggccgccg acgagcgcgg ggagctcgag gacttcctcg cctccagggg cttccggcgc
     1621 tacgaggtct ccaactacgc cccgccgggg gccgagtccc tgcacaacct cgcctactgg
     1681 aggatggact cctacctggg cgtaggcccg ggcgcggtct ccaccctgca ggcggcgggg
     1741 ccgcccggcg cctcgctcag gatcgaggag gagccctccc tggaggccta cgcgatgggc
     1801 gatcccgccg cgcgggggat cggccgctcc gggacggccg ccccgggagg ccggcccgcg
     1861 atggggctgg agacggcgat cccgccccga gaggccgcct tcgaggccgt catgatgggc
     1921 ttcaggactg tcttcggcgt cgacgcgagg gccttcgagg cccgcttcgg cctcgcccta
     1981 gaggccttcg tgggaaggag cctcgaggcc tgggcgggac gcctcgcgcc ggccgccccc
     2041 tggcccgagg gcagccccgt cgcctccccg cgcccgagcg gggaactcgc cctggacggc
     2101 cgcggcctcg acctcctcaa ccgcttcctc tccgactgcc tcggggaaat cgaggggcct
     2161 tctccttcct cggcctttta ggctacgatt gcggcgcgtc ccggaggttt tcgcttggcc
     2221 atcctctcca tacagtccca tgtcgcctac ggccacgtcg gcaaccgctc ggcggtcttc
     2281 cccctcgagc tcct
//
LOCUS       DAOOSJ010000303         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_38967_length_2088_cov_6.023610, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000303 DAOOSJ010000000
VERSION     DAOOSJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38967_length_2088_cov_6.023610"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1960
                     /locus_tag="PLG14_11635"
     CDS             215..1960
                     /locus_tag="PLG14_11635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOX49829.1"
                     /translation="MPLDLPKDPASVLDWDWTRFKPFYDELAARPLAAAGLDAWMSDW
                     TAVSSLVSEAGTRLKIALDLHADDPALEARYTAFLEGVGEPAQAAEQALKEKLLSSGL
                     EPLGFAIPLRNMRAEASLFRAENLPLLTEHEKTALEYDKVVGSRSVEWEGRELTVTRL
                     VGELSGAPRDVRERGWRLAMERVLSDSGEIDAVWTRLMGIRKGIAANAGLSDYREYAW
                     RQRLRFDYSPADSAAFRDAIEKSVVPAARRVYERRAKRLGVSSLRPWDVSVDVMRQTD
                     INVDCFGKPALRPFKDAAELERKIEGVFDRVDPRVGGYFRTMKEERLYDLDNYKGKTP
                     GAYCTGLNASRRPFVLMNAAGAAGDVDTCLHEMGHAFHTFQCYRNPALRYCQLWDYPA
                     EFAEVASMGMELLGSPYLTKDQGAFYTEEEAARAAIMHLEQFLLFWPFMAVVDGFQHW
                     AYEAGDEGAEPQACDAAWAGLWDRFIQGVDWSGLEKAKAKGWQRKLHPFHYPFYYVEY
                     GFAQLGATQIYMRYLKDRAQAVTDYLRGLSLGYSVSLPELFRASGGRFAFDAGTLKEA
                     ADFLEERIAVEEKKL"
BASE COUNT          332 a          765 c          687 g          304 t
ORIGIN      
        1 ctcttcgtcc tcgcctccac ggccaggatc gacgcgatct cggaggcgct gaacgcgccg
       61 cgccggatcc tttccgagcg cgaggccgtc gaggccgaga tagagagcag gcgctagggc
      121 ctgttttcgc ttgacccggc cgcggctggg gggcagactc atccgggccg ggaaggggca
      181 tgacggcctc ctccccgagc caaaaggagt gacgatgccc ctcgacctgc ccaaggatcc
      241 cgcctccgtc ctcgactggg actggacgcg cttcaagcct ttctacgacg aactcgccgc
      301 ccggcccttg gccgccgcgg gcctcgacgc ctggatgtcc gactggaccg ccgtctcctc
      361 cctcgtctcc gaggcgggaa cccgcctcaa gatagccctg gacctccacg ccgacgatcc
      421 cgccctggag gcccgctaca cggccttcct cgagggcgtc ggcgagcccg cccaggcggc
      481 ggagcaggcc ctcaaggaga agctcctctc aagcggcctc gagcccctcg gcttcgccat
      541 acccttgcgc aacatgcgcg ccgaggcctc cctcttccgg gcggagaacc tccccctcct
      601 caccgagcac gagaagacgg ccctcgagta cgacaaggtc gtcggctccc gctccgtgga
      661 gtgggagggc cgcgagctca ccgtgacgcg gctcgtcggc gagctctccg gcgcgccgcg
      721 ggacgtccgc gagaggggct ggcgcctcgc gatggaaagg gtcctctccg actcgggcga
      781 gatcgacgcc gtctggaccc gcctcatggg gatcaggaag gggatcgcgg ccaacgcggg
      841 cctgtccgac taccgggagt acgcctggag gcagcggctc cgcttcgact acagccccgc
      901 cgactcggcc gccttccgcg acgcgataga gaagtcggtc gtgccggccg cgaggcgggt
      961 ctacgaacga cgcgcgaagc gcctgggcgt ctcctccctg aggccctggg acgtctccgt
     1021 cgacgtgatg cgccagaccg acatcaacgt cgactgcttc ggcaagcccg ccctgcggcc
     1081 cttcaaggac gcggccgagc tcgagcgcaa gatcgagggg gtcttcgacc gcgtggatcc
     1141 ccgcgtgggc ggctacttcc ggacgatgaa ggaggagcgg ctctacgacc tggacaacta
     1201 caaggggaag acgcccggcg cctactgcac gggcctcaac gcctccaggc ggcccttcgt
     1261 cctcatgaac gccgcggggg cggccggcga cgtcgacacc tgcctccacg agatgggcca
     1321 cgccttccac accttccagt gctaccggaa ccccgccctg cgctactgcc agctctggga
     1381 ctaccccgcg gagttcgccg aggtggcctc catgggcatg gagctcctcg gctcgcccta
     1441 cctgacgaag gaccagggcg ccttctatac cgaggaggag gccgcccgcg ccgccatcat
     1501 gcacctggag cagttcctcc tcttctggcc cttcatggcc gtcgtcgacg gcttccagca
     1561 ctgggcctac gaggcggggg acgagggcgc cgagcctcag gcctgcgacg ccgcctgggc
     1621 ggggctctgg gaccgcttca tccagggcgt ggactggagc ggcctggaga aggccaaggc
     1681 gaagggctgg cagcggaagc tccacccctt ccactatccc ttctactacg tcgagtacgg
     1741 cttcgcccag ctcggcgcga cgcagatcta catgcgctac ctcaaggacc gagcccaggc
     1801 ggtgaccgac tacctcaggg gcctctcgct cggctactca gtctccctgc ctgagctctt
     1861 ccgggcctcg ggcggccgct tcgccttcga cgcaggcacc ctcaaggagg cggcggactt
     1921 cctggaggag aggatcgccg tcgaggagaa gaagctctag cagggaggag tccccacgca
     1981 gccgtcgcca aggcccggcc gcgcgggggc ttcttcaatc gaggtccgcg aacagctccc
     2041 cgggatcgac ggagaagccc tcgacgacca gggaggctat cggcgaat
//
LOCUS       DAOOSJ010000304         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_40665_length_2034_cov_6.759980, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000304 DAOOSJ010000000
VERSION     DAOOSJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40665_length_2034_cov_6.759980"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLG14_11640"
     CDS             <1..384
                     /locus_tag="PLG14_11640"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOX49830.1"
                     /translation="VNWFRKGKDGEFLWPGFGQNIRVLKWIVDRANNHAKGVESPLGW
                     MPRYEDICWKGLEGFSEEKFGELMAVDRDQWVGELLSHEELFTKLYDRLPKELIFIRE
                     LTLSSIWRSPEHWEFNQVSLEPTED"
     gene            complement(504..1709)
                     /locus_tag="PLG14_11645"
     CDS             complement(504..1709)
                     /locus_tag="PLG14_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX49831.1"
                     /translation="MARDFDLKDFLKRNGSMATMLLVVIFVGMGEKMAERFLPLYLIA
                     LGGSTWSVGFLNSMDNLLSALYSFPGGYLSDRLGFKKSLQLFTGIAMLGYLLVIVVPS
                     WQAVLVGAVFFIAWTAVSLPAIMSMVSKVVPKDRRALGVSMHSFTKRIPMALGPIVGG
                     ALIGAYGRVEGVRIAFGAAFAMGLAALFLMQRFLADDKAGKDSGKRKEIKAKDLFSPA
                     LRSLLASDILIRFAEQIPYAFVVIWAVDSNRLTALQFGLLTTIEMVTAMLVYVPVAWM
                     SDKYGKKKFVLITFGFFTIFPLVLLFSRSFPMFVVAFVIRGLKEFGEPTRKALIMDLA
                     PEDAKARTFGAYYLVRDVIVSVAALSSALLWNISPAANFLTAFGFGVLGTVLFAIFGR
                     DIQAGPRES"
     gene            complement(1713..>2034)
                     /locus_tag="PLG14_11650"
     CDS             complement(1713..>2034)
                     /locus_tag="PLG14_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX49832.1"
                     /translation="IEEAGEIPTLELGKRSEVPPATLIGILDDLERRGLVRRLRSTED
                     KRVVLVSATASGRELVALREEEERRFSEKVDGGLSPAERGALAALLSKLLGGIGDEAG
                     LFKE"
BASE COUNT          364 a          666 c          714 g          290 t
ORIGIN      
        1 gtcaactggt tccgcaaggg caaggacggc gagttcctct ggccgggctt cggccagaac
       61 atccgcgtcc tcaagtggat cgtcgaccgc gcgaacaacc acgcgaaggg cgtggagagc
      121 cccctcggct ggatgccccg ctacgaggac atctgctgga agggcctgga aggcttctcc
      181 gaggagaagt tcggcgagct catggcggtc gaccgcgacc agtgggtcgg cgagctcctc
      241 tcccacgagg agctcttcac gaagctctac gaccgcctcc cgaaggagct catcttcatc
      301 cgcgagctca ccctctccag catctggcgc tcgccggagc actgggagtt caaccaggtg
      361 tcgctggagc cgacggagga ctgaccactc cccgggcggc cgggaggggg gcggccgccc
      421 tcctcccggc cgccgctttg cgcgggaccg cgccgcccgc gggccgcagc cggccgcggg
      481 cgccagcccc cggccctcgc gcctcagctc tcccgcggcc cggcctggat gtccctgccg
      541 aagatcgcga agaggacggt gcccaggact ccgaagccga aggcggtcag gaaattcgcc
      601 gcgggggaga tgttccacag gagggcgctg gagagcgccg cgacggagac gatcacgtcg
      661 cggacgaggt agtaggcgcc gaaggtgcgg gccttcgcgt cctccggcgc gaggtccatg
      721 atgagggcct tgcgcgtcgg ctccccgaat tccttgaggc ccctgatgac gaaggccacg
      781 acgaacatcg ggaaggagcg cgagaagagg aggacgaggg ggaagatggt gaagaagccg
      841 aaggtgatga ggacgaattt cttcttcccg tacttgtcgg acatccaggc gaccggcacg
      901 tagacgagca tcgcggtgac catctcgatc gtcgtgagga ggccgaactg aagggccgtg
      961 aggcggttcg agtcgacggc ccagatgacg acgaaggcgt aggggatctg ctcggcgaaa
     1021 cggatgagga tgtcgctcgc caggaggctg cgcagggccg ggctgaagag gtccttcgcc
     1081 ttgatctcct tccgcttccc ggagtccttc ccggccttgt cgtcggccaa gaacctctgc
     1141 atgaggaaga gggcggcaag gcccatcgcg aaggcggcgc cgaaggcgat gcgcacgccc
     1201 tccacccgcc cgtaggcccc gatgagggct ccgccgacga tgggccccag ggccatcggg
     1261 atgcgcttcg tgaaggagtg catcgacacg cccagggccc gcctgtcctt gggcaccacc
     1321 ttggagacca tgctcatgat cgcgggcagg gagacggccg tccaggcgat gaagaagacc
     1381 gcgccgacga ggaccgcctg ccaggagggg acgacgatca cgaggagata gccgagcatc
     1441 gcgatccccg tgaagagctg cagcgacttc ttgaagccca ggcggtcgga gaggtatccg
     1501 ccggggaagg agtagagggc ggagaggagg ttgtccatcg agttgaggaa gccgacggac
     1561 caggtcgagc cgcccagggc tatgaggtag agcggcagga agcgctccgc catcttctcg
     1621 cccatgccga cgaagatcac gacgaggagc atcgtggcca tgctgccgtt gcgcttcagg
     1681 aaatccttca ggtcgaaatc gcgcgccatg tcctactcct tgaaaagccc ggcctcatcg
     1741 ccgatgccgc ccagcagctt ggagagcagc gccgcgaggg cgccgcgctc ggcgggggag
     1801 aggccgccgt ccaccttctc ggagaagcgc cgctcctcct cctcccgcag ggcgaccagc
     1861 tcccgccccg acgccgtcgc gctcacgagg acgacccgct tgtcctccgt cgagcggagc
     1921 ctccgcacga gtccgcggcg ctccaggtcg tccaggatgc cgatgagggt ggcgggaggg
     1981 acctcgctcc gctttccgag ctcgagggtc ggtatctcgc ccgcctcctc gatg
//
LOCUS       DAOOSJ010000305         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_41882_length_1999_cov_4.683642, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000305 DAOOSJ010000000
VERSION     DAOOSJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41882_length_1999_cov_4.683642"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLG14_11655"
     CDS             complement(<1..1073)
                     /locus_tag="PLG14_11655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX49833.1"
                     /translation="MVKILDVEADPDEAATLAALLADEFRLIHCPDPARAAQAVRREA
                     PAAILFDPARYAEAGHRALEALARSREGPPVIVMSARAEPSRVLCALRRGAYDFLARP
                     CGLHELKSALRSAVEARTYACAPFTGSSPALRKAAALLADYARHEYPVLILGESGTGK
                     DVAARALHEASPRRGGPFVARNCAALPDELIESELFGSDKGAFTGAVERPGAFELAEG
                     GTLFLDEIGEAGPAVQAKLLRALESGEVWRLGGRSSRKADIRLVTATSRDIRGAAEAG
                     RFRPDLLYRIDTLVLELPPLRDRPEDIVFLAESFAREATRGRKSLRPAALERLKAAPW
                     PGNIRQLKNVVHRAVVLSGDLEE"
     gene            complement(1399..>1999)
                     /locus_tag="PLG14_11660"
     CDS             complement(1399..>1999)
                     /locus_tag="PLG14_11660"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOX49834.1"
                     /translation="RARRNGEYRTMIAGASLVVPVSLSIVRGARFLRRAEPVRYMPFE
                     FVVKLLGALERKGKSAYLLGSSRTKLQKAETNIKSTFPGLRVVGRYAGRYPKNVEPAI
                     IEAVRKATPTLLLVGAGVPGRERWIPRNLPRFNSGLYLWCSDLFEVFAESRGKPSREL
                     FRRGLEWLPYTLRRPWKAYRFFVFLWYQVLLLFARIRGR"
BASE COUNT          269 a          673 c          789 g          268 t
ORIGIN      
        1 atctcctcga ggtcgcccga gaggacgacc gcccgatgga cgacgttctt gagttgcctg
       61 atgttgccgg gccagggggc ggccttcagt ctctccaggg cggcgggcct taggctcttc
      121 cggccccgcg tcgcctcgcg ggcgaaggac tccgcgagga aaactatgtc ctccggccgg
      181 tcgcggaggg gcggaagctc caggacgagg gtgtcgatcc ggtagaggag gtcgggccgg
      241 aagcggcccg cctcggccgc gccgcggatg tcccgcgagg tggccgtgac gaggcggatg
      301 tcggccttcc ggctcgagcg ccctcccagt cgccagacct cgcccgactc cagggcgcgg
      361 aggagcttgg cctggacggc cggtccggcc tcgcctatct cgtcgagaaa gagggtccct
      421 ccctccgcga gctcgaaggc gcccggccgc tcgaccgcgc ccgtgaaggc tcccttgtcg
      481 gagccgaaaa gctcgctctc gatgagctcg tcggggaggg cggcgcagtt cctggccacg
      541 aaggggccgc cgcggcgggg cgaggcctcg tggagggccc tcgccgccac gtccttgccc
      601 gtgccgctct cgcccaggat gaggacgggg tactcgtggc gggcgtagtc cgcgaggaga
      661 gcggcggcct tgcggagggc ggggctcgag ccggtgaagg gcgcgcaggc gtaggtccgg
      721 gcctcgacgg ccgagcgcag ggccgacttg agctcgtgga ggccgcaggg cctggccagg
      781 aagtcgtagg cgccccgcct gagggcgcag aggacccggc tgggctcggc cctggcgctc
      841 atcacgatga cgggcggccc ctccctggaa cgcgccaggg cttcgagggc gcggtgcccg
      901 gcctccgcgt agcgcgcggg gtcgaagagg attgccgcgg gggcctcgcg cctcacggcc
      961 tgggccgccc tggcgggatc ggggcagtgg atgagccgga actcgtcggc gagcagggct
     1021 gcgagggtag ccgcctcgtc cgggtcggcc tccacgtcga gtattttgac catcgtggga
     1081 tagagtctcg cgccaggcca gccgccgcac aacgcgggga gggacgcgcc ggcgtttcgg
     1141 ccttgtagac aatcgtctca aaggcttaat gggcgttggg ctcatcgctt ccgcccggtc
     1201 cgtcccatgg ctctcaggca ggggccgctt tcccggggag ggcgggccgc cgggacggcc
     1261 gccctccggg acggcgaggg cggcccggtc gccgcaacac ggccccgccg cgacgcggcg
     1321 ctccggccgc ggcgccgcgg cgccatgtcg ccccggccgc ggccgccctg ggcgccaggc
     1381 cgcccccggc ccagccgcct agcggccgcg gatgcgggcg aagaggagga ggacctggta
     1441 ccagaggaag acgaagaagc ggtaggcctt ccaggggcgg cgcagggtgt agggcagcca
     1501 ctcgagtccc cggcggaaga gctcgcggga gggcttgccc cggctctcgg cgaagacctc
     1561 gaagaggtcg gagcaccaga ggtagaggcc cgagttgaag cggggcaggt tcctggggat
     1621 ccagcgctcg cgtccgggga cgccggctcc gacgaggagg agggtcggcg tcgccttcct
     1681 gaccgcctcg atgatcgcgg gctcgacgtt cttcgggtag cggccggcgt agcggccgac
     1741 gaccctgagg cccgggaagg tggacttgat gttggtctcc gccttctgga gcttggtccg
     1801 cgaggagccg aggaggtagg cggacttgcc cttccgctcc agggccccga ggagcttcac
     1861 gacgaactcg aagggcatgt agcgcacggg ctcggcccgg cggaggaagc gggctccccg
     1921 cacgatggag agggagacgg gcacgaccag gctcgccccg gcgatcatgg ttctgtactc
     1981 gccgttccgc ctggcgcgc
//
LOCUS       DAOOSJ010000306         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_2522
            NODE_42037_length_1995_cov_4.529897, whole genome shotgun sequence.
ACCESSION   DAOOSJ010000306 DAOOSJ010000000
VERSION     DAOOSJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864597
            Sequence Read Archive: SRR4244739
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,335
            CDSs (total)                      :: 2,311
            Genes (coding)                    :: 2,302
            CDSs (with protein)               :: 2,302
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42037_length_1995_cov_4.529897"
                     /isolate="AMR_MDS_2522"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLG14_11665"
     CDS             <1..577
                     /locus_tag="PLG14_11665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX49835.1"
                     /translation="IVNLSDYLDTGLFLDHRPARAMVRAESEGKRVLNLFAYTGSFTV
                     YAASGGAAEATSVDLSNTYLDWAERNLLANGFSGPAYPLVRAEVRAFLAAAARKGERW
                     DLIVADPPTFSNSKASPEDFDVNRDWLALVESCAALLSPGGVLYFSSNSRRLPFDASR
                     VPLPCEDITEKSLPPDFRDRRAHRAWRIAAP"
     gene            711..1127
                     /locus_tag="PLG14_11670"
     CDS             711..1127
                     /locus_tag="PLG14_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOX49836.1"
                     /translation="MRVTTKGRYALRAVLALARTSGEGNPVSIKSIATQEEISAEFLE
                     QIFFRLRRAGVISSVRGPGGGFYFSKPLAEITLLDILEASGEGLGISPCACGKKDDCD
                     KGDSCAAGAIWKEMDLHLREFVEGKTLEDMLRDERN"
     gene            complement(1250..>1995)
                     /gene="pyrE"
                     /locus_tag="PLG14_11675"
     CDS             complement(1250..>1995)
                     /gene="pyrE"
                     /locus_tag="PLG14_11675"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOX49837.1"
                     /translation="EAADPAAAARELRDAMEAARLAPPAASPSAPGFGPGVAPKASAG
                     PAQATAALKEAFIEALVRTECFKLGDFVLKSGKRSPFYIDLRRLVSDPKALALAGQAY
                     ASLAEGLAFDRIAGIPAAALPLATAACLAVGKPMVWPRMPVKEHGTGNRVEGAFAAGE
                     RLLLLDDLITTGASKLEAIAILRGEGLVVEELAVLIERGKQGRRDMEAAGVALRAFIH
                     VRELFAVCERLGLVDPARRRELEAFVDAE"
BASE COUNT          293 a          730 c          668 g          304 t
ORIGIN      
        1 catagtcaac ctctccgact acctcgacac gggcctcttc ctggaccatc gcccagcgag
       61 ggccatggtc agggcggagt cggaggggaa gcgcgtcctc aacctcttcg cctacacggg
      121 aagcttcacc gtctacgccg cctcaggcgg cgcggctgag gcgacctccg tggacctctc
      181 gaacacctac ctggactggg ccgagcggaa cctcctcgcg aacggcttct ccgggcccgc
      241 ctaccccctg gtcagggccg aggtcagggc cttcctggcg gccgcggcgc ggaagggcga
      301 gcgctgggac ctcatcgtcg ccgatccccc caccttctcg aactcgaagg cctcgcccga
      361 ggacttcgac gtgaaccggg actggctcgc cctcgtggag tcctgcgccg ccctcctctc
      421 gccgggcggc gtcctctact tctcctccaa ttcccgccgc ctccccttcg acgcctcccg
      481 cgtccccctg ccgtgcgagg acatcaccga gaagagcctt ccgcccgact tccgcgacag
      541 gcgcgcccac cgggcctggc gcatcgccgc tccctagccc ccggcgatcc gccgcgggcc
      601 gccggcctcc gcgtgttatc aattattagc atcggttttc ccgatcccgc ttgcgccttt
      661 cgcgccggcg cggtactatg tgcctacgat aacaatttaa ggagagcatc ttgcgcgtca
      721 ccacgaaggg tcggtatgcg ctgcgcgcgg tccttgcgct cgcgaggacc tcgggagaag
      781 gcaaccccgt ttccatcaag tccatagcca cccaggagga gatctccgcc gagttcctgg
      841 agcagatttt cttcaggctg aggagggccg gcgtcatcag ctcggtgcgc gggcccgggg
      901 gcggcttcta cttctcgaaa cccctcgccg agatcaccct cctcgacatc ctggaggcct
      961 cgggcgaagg cctgggcatc tcgccctgcg cctgcggcaa gaaggacgac tgcgacaagg
     1021 gcgacagttg cgcggccggc gcgatctgga aggagatgga cctccacctc cgcgagttcg
     1081 tcgagggcaa gaccctggag gacatgctcc gggacgagcg caactagcgg ctaaggaaaa
     1141 gcggggccgc gcctccatgc gtcgccggtc ccaataacgg aaaagggccg cccgggaagc
     1201 tattcccggg cggccctcct cgtatccgcg gctattcctc gtacagcggc tactccgcgt
     1261 cgacgaaggc ctcgagctcg cgccgccggg ccggatcgac gaggccgagc ctctcgcaga
     1321 ccgcgaagag ctcgcggacg tggatgaagg cccggagcgc gacgcccgcc gcctccatgt
     1381 cccgcctgcc ctgcttcccc cgctcgatga ggacggccag ctcctcaacg accaggccct
     1441 ctccgcggag gatggcgatg gcctcgagct tgctcgcccc cgtcgtgatg aggtcgtcca
     1501 ggaggaggag gcgctcgccc gcggcgaagg ccccttcgac gcggttgccc gtgccgtgct
     1561 ccttcaccgg catccggggc cagaccatgg gcttcccgac cgcgaggcag gccgccgtcg
     1621 cgagggggag ggccgcggcc gggatgcccg cgatgcggtc gaaggcgagg ccctcggcga
     1681 ggctcgcgta ggcctgcccg gccagggcca gggccttcgg gtccgagacg aggcgccgca
     1741 agtcgatgta gaaggggctc cgcttcccgc ttttcaggac gaagtccccg agcttgaagc
     1801 actcggtcct gaccaaggcc tcgatgaagg cttctttgag ggcggcggtc gcttgggccg
     1861 gccccgcgga cgccttgggc gctacgccgg ggccgaagcc gggggccgag ggggaggcgg
     1921 ccggcggggc caggcgggcg gcctccatcg cgtcgcggag ctcccgggcc gcggcggctg
     1981 gatcggcggc ctccg
//
